LOCUS       DARBFO010000001       180795 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000001 DARBFO010000000
VERSION     DARBFO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 180795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 180795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..180795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.001"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(12..1535)
                     /locus_tag="SCK51_000001"
     CDS             complement(12..1535)
                     /locus_tag="SCK51_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEG1250662.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="SCK51_000002"
     CDS             1787..2968
                     /locus_tag="SCK51_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1250663.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="SCK51_000003"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="SCK51_000003"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEG1250664.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="SCK51_000004"
     CDS             complement(5053..7764)
                     /locus_tag="SCK51_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1250665.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="SCK51_000005"
     CDS             complement(7774..8475)
                     /locus_tag="SCK51_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1250666.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="SCK51_000006"
     CDS             8661..9260
                     /locus_tag="SCK51_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1250667.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="SCK51_000007"
     CDS             9341..10354
                     /locus_tag="SCK51_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG1250668.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="SCK51_000008"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="SCK51_000008"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEG1250669.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="SCK51_000009"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="SCK51_000009"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEG1250670.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="SCK51_000010"
     CDS             11802..13175
                     /locus_tag="SCK51_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1250671.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="SCK51_000011"
     CDS             13532..15676
                     /locus_tag="SCK51_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEG1250672.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="SCK51_000012"
     CDS             complement(15728..16768)
                     /locus_tag="SCK51_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG1250673.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="SCK51_000013"
     CDS             complement(17045..18847)
                     /locus_tag="SCK51_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1250674.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="SCK51_000014"
     CDS             complement(18861..19613)
                     /locus_tag="SCK51_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1250675.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="SCK51_000015"
     CDS             complement(19622..19984)
                     /locus_tag="SCK51_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1250676.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="SCK51_000016"
     CDS             complement(20160..21032)
                     /locus_tag="SCK51_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEG1250677.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="SCK51_000017"
     CDS             complement(21058..21570)
                     /locus_tag="SCK51_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEG1250678.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22499)
                     /locus_tag="SCK51_000018"
     CDS             complement(21708..22499)
                     /locus_tag="SCK51_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEG1250679.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22554..23162)
                     /locus_tag="SCK51_000019"
     CDS             complement(22554..23162)
                     /locus_tag="SCK51_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEG1250680.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="SCK51_000020"
     CDS             complement(23155..23472)
                     /locus_tag="SCK51_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1250681.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="SCK51_000021"
     CDS             complement(23628..24356)
                     /locus_tag="SCK51_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEG1250682.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="SCK51_000022"
     CDS             24515..25396
                     /locus_tag="SCK51_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1250683.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="SCK51_000023"
     CDS             complement(25633..26676)
                     /locus_tag="SCK51_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1250684.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="SCK51_000024"
     CDS             complement(26679..27347)
                     /locus_tag="SCK51_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1250685.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="SCK51_000025"
     CDS             27489..29825
                     /locus_tag="SCK51_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1250686.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="SCK51_000026"
     CDS             complement(29940..30251)
                     /locus_tag="SCK51_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250687.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="SCK51_000027"
     CDS             30432..31907
                     /locus_tag="SCK51_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEG1250688.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="SCK51_000028"
     CDS             31954..32538
                     /locus_tag="SCK51_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEG1250689.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="SCK51_000029"
     CDS             32540..32959
                     /locus_tag="SCK51_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEG1250690.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="SCK51_000030"
     CDS             complement(33240..33524)
                     /locus_tag="SCK51_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEG1250691.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="SCK51_000031"
     CDS             complement(33876..34586)
                     /locus_tag="SCK51_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEG1250692.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="SCK51_000032"
     CDS             complement(34583..36055)
                     /locus_tag="SCK51_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG1250693.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="SCK51_000033"
     CDS             36193..36666
                     /locus_tag="SCK51_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1250694.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="SCK51_000034"
     CDS             complement(36819..37370)
                     /locus_tag="SCK51_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1250695.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="SCK51_000035"
     CDS             37475..38005
                     /locus_tag="SCK51_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEG1250696.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="SCK51_000036"
     CDS             complement(38488..38931)
                     /locus_tag="SCK51_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1250697.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="SCK51_000037"
     CDS             complement(38947..40107)
                     /locus_tag="SCK51_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1250698.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="SCK51_000038"
     CDS             40614..42314
                     /locus_tag="SCK51_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250699.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="SCK51_000039"
     CDS             complement(42416..43249)
                     /locus_tag="SCK51_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEG1250700.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="SCK51_000040"
     CDS             complement(43553..45718)
                     /locus_tag="SCK51_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1250701.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="SCK51_000041"
     CDS             45978..48272
                     /locus_tag="SCK51_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEG1250702.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="SCK51_000042"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="SCK51_000042"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEG1250703.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="SCK51_000043"
     CDS             48923..49930
                     /locus_tag="SCK51_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG1250704.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="SCK51_000044"
     CDS             49952..50971
                     /locus_tag="SCK51_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1250705.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="SCK51_000045"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="SCK51_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEG1250706.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /locus_tag="SCK51_000046"
     CDS             complement(52729..54150)
                     /locus_tag="SCK51_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1250707.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="SCK51_000047"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="SCK51_000047"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEG1250708.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /locus_tag="SCK51_000048"
     CDS             complement(56561..57817)
                     /locus_tag="SCK51_000048"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEG1250709.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57929..58134)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58397..59698
                     /locus_tag="SCK51_000049"
     CDS             58397..59698
                     /locus_tag="SCK51_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEG1250710.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="SCK51_000050"
     CDS             complement(59767..60366)
                     /locus_tag="SCK51_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1250711.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60620..61501
                     /locus_tag="SCK51_000051"
     CDS             60620..61501
                     /locus_tag="SCK51_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1250712.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61514..63124
                     /locus_tag="SCK51_000052"
     CDS             61514..63124
                     /locus_tag="SCK51_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEG1250713.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63201..63860)
                     /locus_tag="SCK51_000053"
     CDS             complement(63201..63860)
                     /locus_tag="SCK51_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG1250714.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64143..64808)
                     /locus_tag="SCK51_000054"
     CDS             complement(64143..64808)
                     /locus_tag="SCK51_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEG1250715.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="SCK51_000055"
     CDS             complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="SCK51_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEG1250716.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="SCK51_000056"
     CDS             complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="SCK51_000056"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEG1250717.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(66573..67019)
                     /gene="dhaM"
                     /locus_tag="SCK51_000057"
     CDS             complement(66573..67019)
                     /gene="dhaM"
                     /locus_tag="SCK51_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEG1250718.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            67122..67442
                     /locus_tag="SCK51_000058"
     CDS             67122..67442
                     /locus_tag="SCK51_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEG1250719.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(67554..68423)
                     /locus_tag="SCK51_000059"
     CDS             complement(67554..68423)
                     /locus_tag="SCK51_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1250720.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(68519..69822)
                     /locus_tag="SCK51_000060"
                     /pseudo
     CDS             complement(68519..69822)
                     /locus_tag="SCK51_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(69819..70328)
                     /locus_tag="SCK51_000061"
     CDS             complement(69819..70328)
                     /locus_tag="SCK51_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250721.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(70503..72638)
                     /locus_tag="SCK51_000062"
     CDS             complement(70503..72638)
                     /locus_tag="SCK51_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1250722.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(72721..74376)
                     /locus_tag="SCK51_000063"
     CDS             complement(72721..74376)
                     /locus_tag="SCK51_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1250723.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(74378..74692)
                     /locus_tag="SCK51_000064"
     CDS             complement(74378..74692)
                     /locus_tag="SCK51_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG1250724.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            74862..75122
                     /locus_tag="SCK51_000065"
     CDS             74862..75122
                     /locus_tag="SCK51_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEG1250725.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            75136..75450
                     /locus_tag="SCK51_000066"
     CDS             75136..75450
                     /locus_tag="SCK51_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEG1250726.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(76180..76734)
                     /locus_tag="SCK51_000067"
     CDS             complement(76180..76734)
                     /locus_tag="SCK51_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEG1250727.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(76860..78788)
                     /locus_tag="SCK51_000068"
     CDS             complement(76860..78788)
                     /locus_tag="SCK51_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEG1250728.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(79094..79810)
                     /locus_tag="SCK51_000069"
     CDS             complement(79094..79810)
                     /locus_tag="SCK51_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEG1250729.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="SCK51_000070"
     CDS             complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="SCK51_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEG1250730.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="SCK51_000071"
     CDS             complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="SCK51_000071"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEG1250731.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(83280..84740)
                     /locus_tag="SCK51_000072"
     CDS             complement(83280..84740)
                     /locus_tag="SCK51_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1250732.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(84730..86025)
                     /locus_tag="SCK51_000073"
     CDS             complement(84730..86025)
                     /locus_tag="SCK51_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1250733.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(86357..87034)
                     /locus_tag="SCK51_000074"
     CDS             complement(86357..87034)
                     /locus_tag="SCK51_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEG1250734.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(87064..88155)
                     /locus_tag="SCK51_000075"
     CDS             complement(87064..88155)
                     /locus_tag="SCK51_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEG1250735.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(88256..88690)
                     /locus_tag="SCK51_000076"
     CDS             complement(88256..88690)
                     /locus_tag="SCK51_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1250736.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="SCK51_000077"
     CDS             complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="SCK51_000077"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG1250737.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(90728..92623)
                     /locus_tag="SCK51_000078"
     CDS             complement(90728..92623)
                     /locus_tag="SCK51_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1250738.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(92681..94456)
                     /locus_tag="SCK51_000079"
     CDS             complement(92681..94456)
                     /locus_tag="SCK51_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEG1250739.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(94506..96002)
                     /locus_tag="SCK51_000080"
     CDS             complement(94506..96002)
                     /locus_tag="SCK51_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1250740.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(96196..97818)
                     /locus_tag="SCK51_000081"
     CDS             complement(96196..97818)
                     /locus_tag="SCK51_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEG1250741.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(98267..99580)
                     /locus_tag="SCK51_000082"
     CDS             complement(98267..99580)
                     /locus_tag="SCK51_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEG1250742.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(99825..100049)
                     /locus_tag="SCK51_000083"
     CDS             complement(99825..100049)
                     /locus_tag="SCK51_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250743.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(100069..100491)
                     /locus_tag="SCK51_000084"
     CDS             complement(100069..100491)
                     /locus_tag="SCK51_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEG1250744.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(100635..101345)
                     /locus_tag="SCK51_000085"
     CDS             complement(100635..101345)
                     /locus_tag="SCK51_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250745.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(101363..101860)
                     /locus_tag="SCK51_000086"
     CDS             complement(101363..101860)
                     /locus_tag="SCK51_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1250746.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(101897..102349)
                     /locus_tag="SCK51_000087"
     CDS             complement(101897..102349)
                     /locus_tag="SCK51_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1250747.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(102484..103089)
                     /locus_tag="SCK51_000088"
     CDS             complement(102484..103089)
                     /locus_tag="SCK51_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG1250748.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(103539..104159)
                     /locus_tag="SCK51_000089"
     CDS             complement(103539..104159)
                     /locus_tag="SCK51_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1250749.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(104161..105030)
                     /locus_tag="SCK51_000090"
     CDS             complement(104161..105030)
                     /locus_tag="SCK51_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEG1250750.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(105053..105460)
                     /locus_tag="SCK51_000091"
     CDS             complement(105053..105460)
                     /locus_tag="SCK51_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEG1250751.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(105479..106768)
                     /locus_tag="SCK51_000092"
     CDS             complement(105479..106768)
                     /locus_tag="SCK51_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1250752.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(106795..107097)
                     /locus_tag="SCK51_000093"
     CDS             complement(106795..107097)
                     /locus_tag="SCK51_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1250753.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(107122..109215)
                     /locus_tag="SCK51_000094"
     CDS             complement(107122..109215)
                     /locus_tag="SCK51_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1250754.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(109704..110468)
                     /locus_tag="SCK51_000095"
     CDS             complement(109704..110468)
                     /locus_tag="SCK51_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEG1250755.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="SCK51_000096"
     CDS             complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="SCK51_000096"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEG1250756.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(111473..112549)
                     /locus_tag="SCK51_000097"
     CDS             complement(111473..112549)
                     /locus_tag="SCK51_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEG1250757.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(112573..113166)
                     /locus_tag="SCK51_000098"
     CDS             complement(112573..113166)
                     /locus_tag="SCK51_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HEG1250758.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="SCK51_000099"
     CDS             complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="SCK51_000099"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEG1250759.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(114836..115135)
                     /locus_tag="SCK51_000100"
     CDS             complement(114836..115135)
                     /locus_tag="SCK51_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HEG1250760.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="SCK51_000101"
     CDS             complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="SCK51_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEG1250761.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(116890..117801)
                     /locus_tag="SCK51_000102"
     CDS             complement(116890..117801)
                     /locus_tag="SCK51_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEG1250762.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            117921..118778
                     /locus_tag="SCK51_000103"
     CDS             117921..118778
                     /locus_tag="SCK51_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1250763.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(118965..119327)
                     /locus_tag="SCK51_000104"
     CDS             complement(118965..119327)
                     /locus_tag="SCK51_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEG1250764.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(119516..119698)
                     /locus_tag="SCK51_000105"
     CDS             complement(119516..119698)
                     /locus_tag="SCK51_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250765.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            119673..121112
                     /locus_tag="SCK51_000106"
     CDS             119673..121112
                     /locus_tag="SCK51_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1250766.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(121119..123671)
                     /locus_tag="SCK51_000107"
     CDS             complement(121119..123671)
                     /locus_tag="SCK51_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEG1250767.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(123785..125116)
                     /locus_tag="SCK51_000108"
     CDS             complement(123785..125116)
                     /locus_tag="SCK51_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1250768.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(125082..126560)
                     /locus_tag="SCK51_000109"
     CDS             complement(125082..126560)
                     /locus_tag="SCK51_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1250769.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="SCK51_000110"
     CDS             complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="SCK51_000110"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEG1250770.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            129170..130597
                     /locus_tag="SCK51_000111"
     CDS             129170..130597
                     /locus_tag="SCK51_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1250771.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(130689..131054)
                     /locus_tag="SCK51_000112"
     CDS             complement(130689..131054)
                     /locus_tag="SCK51_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEG1250772.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(131051..131416)
                     /locus_tag="SCK51_000113"
     CDS             complement(131051..131416)
                     /locus_tag="SCK51_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEG1250773.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(131608..131856)
                     /locus_tag="SCK51_000114"
     CDS             complement(131608..131856)
                     /locus_tag="SCK51_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250774.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            131855..131983
                     /locus_tag="SCK51_000115"
     CDS             131855..131983
                     /locus_tag="SCK51_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEG1250775.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            132040..133734
                     /locus_tag="SCK51_000116"
     CDS             132040..133734
                     /locus_tag="SCK51_000116"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEG1250776.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            133738..133857
                     /locus_tag="SCK51_000117"
                     /pseudo
     CDS             133738..133857
                     /locus_tag="SCK51_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            134385..135518
                     /locus_tag="SCK51_000118"
     CDS             134385..135518
                     /locus_tag="SCK51_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEG1250777.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            135549..135869
                     /locus_tag="SCK51_000119"
     CDS             135549..135869
                     /locus_tag="SCK51_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HEG1250778.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(135898..136362)
                     /locus_tag="SCK51_000120"
     CDS             complement(135898..136362)
                     /locus_tag="SCK51_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1250779.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(136494..137747)
                     /locus_tag="SCK51_000121"
     CDS             complement(136494..137747)
                     /locus_tag="SCK51_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1250780.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(137776..138093)
                     /locus_tag="SCK51_000122"
     CDS             complement(137776..138093)
                     /locus_tag="SCK51_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1250781.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(138119..138439)
                     /locus_tag="SCK51_000123"
     CDS             complement(138119..138439)
                     /locus_tag="SCK51_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1250782.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(138903..140318)
                     /locus_tag="SCK51_000124"
     CDS             complement(138903..140318)
                     /locus_tag="SCK51_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1250783.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(140335..141651)
                     /locus_tag="SCK51_000125"
     CDS             complement(140335..141651)
                     /locus_tag="SCK51_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1250784.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(141801..142499)
                     /locus_tag="SCK51_000126"
     CDS             complement(141801..142499)
                     /locus_tag="SCK51_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEG1250785.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(142788..143744)
                     /locus_tag="SCK51_000127"
     CDS             complement(142788..143744)
                     /locus_tag="SCK51_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1250786.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(143857..144690)
                     /gene="msrA"
                     /locus_tag="SCK51_000128"
     CDS             complement(143857..144690)
                     /gene="msrA"
                     /locus_tag="SCK51_000128"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG1250787.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(144865..146499)
                     /locus_tag="SCK51_000129"
     CDS             complement(144865..146499)
                     /locus_tag="SCK51_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250788.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(146486..148354)
                     /locus_tag="SCK51_000130"
     CDS             complement(146486..148354)
                     /locus_tag="SCK51_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250789.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVL
                     YYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIK
                     FVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNN
                     DITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPIS
                     LLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAK
                     VIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKY
                     KAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENK
                     IEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            148320..149957
                     /locus_tag="SCK51_000131"
     CDS             148320..149957
                     /locus_tag="SCK51_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250790.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(150102..150842)
                     /gene="truA"
                     /locus_tag="SCK51_000132"
     CDS             complement(150102..150842)
                     /gene="truA"
                     /locus_tag="SCK51_000132"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEG1250791.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(151033..151668)
                     /locus_tag="SCK51_000133"
     CDS             complement(151033..151668)
                     /locus_tag="SCK51_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEG1250792.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(151815..153056)
                     /gene="sstT"
                     /locus_tag="SCK51_000134"
     CDS             complement(151815..153056)
                     /gene="sstT"
                     /locus_tag="SCK51_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEG1250793.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            153254..154252
                     /locus_tag="SCK51_000135"
     CDS             153254..154252
                     /locus_tag="SCK51_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250794.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            154265..156283
                     /locus_tag="SCK51_000136"
     CDS             154265..156283
                     /locus_tag="SCK51_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEG1250795.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(156343..157059)
                     /locus_tag="SCK51_000137"
     CDS             complement(156343..157059)
                     /locus_tag="SCK51_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEG1250796.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(157286..157828)
                     /locus_tag="SCK51_000138"
     CDS             complement(157286..157828)
                     /locus_tag="SCK51_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEG1250797.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(157833..158060)
                     /locus_tag="SCK51_000139"
     CDS             complement(157833..158060)
                     /locus_tag="SCK51_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG1250798.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(158139..160019)
                     /locus_tag="SCK51_000140"
     CDS             complement(158139..160019)
                     /locus_tag="SCK51_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEG1250799.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(160208..160822)
                     /locus_tag="SCK51_000141"
     CDS             complement(160208..160822)
                     /locus_tag="SCK51_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1250800.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            160964..161524
                     /locus_tag="SCK51_000142"
     CDS             160964..161524
                     /locus_tag="SCK51_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1250801.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(161829..162143)
                     /locus_tag="SCK51_000143"
                     /pseudo
     CDS             complement(161829..162143)
                     /locus_tag="SCK51_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(162397..164032)
                     /locus_tag="SCK51_000144"
                     /pseudo
     CDS             complement(162397..164032)
                     /locus_tag="SCK51_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(164587..165150)
                     /locus_tag="SCK51_000145"
     CDS             complement(164587..165150)
                     /locus_tag="SCK51_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG1250802.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(165165..166208)
                     /locus_tag="SCK51_000146"
     CDS             complement(165165..166208)
                     /locus_tag="SCK51_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250803.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(166341..166745)
                     /locus_tag="SCK51_000147"
     CDS             complement(166341..166745)
                     /locus_tag="SCK51_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG1250804.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(166978..167613)
                     /locus_tag="SCK51_000148"
     CDS             complement(166978..167613)
                     /locus_tag="SCK51_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEG1250805.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(167879..168931)
                     /locus_tag="SCK51_000149"
     CDS             complement(167879..168931)
                     /locus_tag="SCK51_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1250806.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(168944..169882)
                     /locus_tag="SCK51_000150"
     CDS             complement(168944..169882)
                     /locus_tag="SCK51_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG1250807.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(170333..170983)
                     /locus_tag="SCK51_000151"
     CDS             complement(170333..170983)
                     /locus_tag="SCK51_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250808.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(171083..171592)
                     /locus_tag="SCK51_000152"
     CDS             complement(171083..171592)
                     /locus_tag="SCK51_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HEG1250809.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(171793..172662)
                     /locus_tag="SCK51_000153"
     CDS             complement(171793..172662)
                     /locus_tag="SCK51_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1250810.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(172682..173803)
                     /locus_tag="SCK51_000154"
     CDS             complement(172682..173803)
                     /locus_tag="SCK51_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEG1250811.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(173805..174200)
                     /locus_tag="SCK51_000155"
     CDS             complement(173805..174200)
                     /locus_tag="SCK51_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG1250812.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(174219..175025)
                     /locus_tag="SCK51_000156"
     CDS             complement(174219..175025)
                     /locus_tag="SCK51_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1250813.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(175018..175806)
                     /locus_tag="SCK51_000157"
     CDS             complement(175018..175806)
                     /locus_tag="SCK51_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1250814.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(175820..176296)
                     /locus_tag="SCK51_000158"
     CDS             complement(175820..176296)
                     /locus_tag="SCK51_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1250815.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(176368..179025)
                     /locus_tag="SCK51_000159"
     CDS             complement(176368..179025)
                     /locus_tag="SCK51_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1250816.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(179044..179448)
                     /locus_tag="SCK51_000160"
     CDS             complement(179044..179448)
                     /locus_tag="SCK51_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HEG1250817.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(179680..179937)
                     /locus_tag="SCK51_000161"
     CDS             complement(179680..179937)
                     /locus_tag="SCK51_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HEG1250818.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(180062..180394)
                     /locus_tag="SCK51_000162"
     CDS             complement(180062..180394)
                     /locus_tag="SCK51_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250819.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT        54128 a        37177 c        30662 g        58828 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60421 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60481 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt
    60541 ataaaaaggt ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag
    60601 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
    60661 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
    60721 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
    60781 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
    60841 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
    60901 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
    60961 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
    61021 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
    61081 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
    61141 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
    61201 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca gtcgtagaag aaggaacact
    61261 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
    61321 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
    61381 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
    61441 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
    61501 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
    61561 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
    61621 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
    61681 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
    61741 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
    61801 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
    61861 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
    61921 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
    61981 agcagattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg
    62041 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
    62101 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
    62161 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
    62221 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
    62281 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
    62341 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
    62401 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
    62461 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
    62521 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtcg tcttgatttg
    62581 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
    62641 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
    62701 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
    62761 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
    62821 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
    62881 atggcttcct cgaatgggta aacttgctta tatctactta gcttactcct ttgcactcaa
    62941 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
    63001 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
    63061 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
    63121 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
    63181 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
    63241 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
    63301 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
    63361 tgatccttcc ctgtcgcttt agctaagtca agcagataga cagcgactcg ttcttctact
    63421 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
    63481 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
    63541 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
    63601 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
    63661 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
    63721 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
    63781 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
    63841 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
    63901 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
    63961 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
    64021 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
    64081 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
    64141 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
    64201 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
    64261 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
    64321 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
    64381 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
    64441 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
    64501 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
    64561 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
    64621 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
    64681 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
    64741 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
    64801 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
    64861 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
    64921 aactgcgatg agcatcaccg cagttttatt gaacttaagg ttgattgatc acatctaaaa
    64981 gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc gatatgtccg attgcacgct
    65041 ctcctagata ggaagctcgt ccttttttag ctaccaagac tttggtgtgt tcttttgcct
    65101 ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc ctctgtgact ggcagccaaa
    65161 catctaacat cgttttatcg tcagcttctg cctttcctcg gctttgaata cccgctgttc
    65221 cctgctcgat cacttcgcct aatttttctt gagaatcaat agaatcgacc tcttttgtcg
    65281 ctttcatcat gttcatgaat gcagatccgt ataacggacc agaagcccca ccgactttcg
    65341 atatcatcgc catagagaat actttgaatg tatcgctgat tgtttcagga tgatcggctt
    65401 gaaaagcttc ttcataagct gaaacccctc ttcccatgtt atttccatgg tctccatcgc
    65461 caatcggggt atctagatca ctgagatatt gtttgttctg attgattgcc tctgcaaatt
    65521 gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat attatcaccc ttttatcttg
    65581 agatttttta ccatgaaatc gtctgtacat tgctttctaa agaatctttc cattgtgtct
    65641 ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc tagtgaggtc ataaaattac
    65701 cgacttttcg gaaaactaca ttgacgcctc tttcttccaa cagagctaaa acatcattca
    65761 tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc gttgactagt acacctgctt
    65821 cttttggtaa attcgagtaa tcatctaaaa tcttagtaac aagttccttt gccagtactt
    65881 tagaaggctg catcttttct cgacgatagc ctggctcacc gtggatacca acaccatact
    65941 cgatctcatc ttctcctagt tcgaatcctg gtttccctac ttctggtaca gttgctgctc
    66001 gaagcgctag accgatcgtc ttagttgcct gtacgacctt ttctcccagt tctttcaact
    66061 cagctaggga agcaccgttt cttgctgcat cgcccacgat tttgtgtaca aggacagtac
    66121 ctgcaactcc gcgttttcca gcagtatacg tactattttc aacggcgata tcatcatcta
    66181 cgatcacact ttctacttca atatcgtcca tagcagctaa ttcctttgcc atatcgaagt
    66241 tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac tccttttcca ctatccgctt
    66301 ccttaatagc cgtctgaatc tgatctggag ttggagaagt aaacacatca ccaagcaccg
    66361 ctgcacttaa catcccttcc cctacaaacc cggcatggga tggttcatgt ccgcttcctc
    66421 caccagaaac caaaccaact tgttccgctt tctgattctt ggctacaaca cgggaagatt
    66481 ctacacgatg aacaagttct ggatagcttt ttactaatcc ttccagcatt tcttcgacaa
    66541 tattgcttgg atcattcatg atttttttca atttattccc atcctttctt tccggcattt
    66601 tgtgcttctg ttaagatttg ctgagtatca tctgttatac cggcagtgat ggctgctgca
    66661 aaagctcctt ccactaaagg cgcatccacc agatgataac gttgctgctg ctcttcttcc
    66721 aacatgtctt ttgccagttc cgagttcaat accgcgctac cgatatccgc aaaaacaaaa
    66781 taactagctg catcgcttga ttcattgact gcttctacga ttttcatcgg gtctgatccg
    66841 atctcgccat ttattccgcc gagagaaaaa atttgcacct cttcggattt ttgcatctgc
    66901 tcaatcattt ctttaatacc atctgttact ttttgactat gagaaataag caaaatactt
    66961 tttttcacaa gaatcttcct ttcttttaca tagttgctaa cgattggttt cgctttcatt
    67021 aatgaacaat tcaagtatat aataaatttc taattttggg aagatatgtg tagtgcttct
    67081 tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg aatgattaca acaacaacga
    67141 acagtgtaga gggaaaagcc gtagtagctt ataaagggat cgtttttggt gaggtcatta
    67201 ctgggatcca tgcatttaaa gacttggagg cggggttgcg aaatatcttt ggcggccgct
    67261 caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga agcacttgcc gaaatggccg
    67321 aacgtgcaaa agcattagga gctgatgcga tcattggcgt aaaaatggac tatgaagtat
    67381 taggcagcga caatggcatg ctgatggtca catgtagtgg aacagctgtc tccacgagat
    67441 aagtagggga catctgtttt aataactaaa gagagatggg acatgtagtg gcactcaaga
    67501 attagatttt tcagtctaac ttcgaggtac tctaccttcc catcctctct tttttatttc
    67561 tttttgcgga tgattccgta agctgctgcg actaacacac ccgcccctgc taaggaataa
    67621 ctgacagaag ctgttttttc atttgttttt ggcaaagtct gacttgttga tttggatggc
    67681 tctgtcgttt gctgaactgt ttctgtcgtg cttccagagg tcactgattt ttctcctgcc
    67741 gaatgcaaaa tatcttttat tcgttggaaa ctagctgcta agtcagcttt ttttgcttca
    67801 aaatcattag caactgtttg tgcttcgctg atctttccgt ggattgcttg actggccgct
    67861 gtgatatccg cttgagtttg acttagcttt ccagaagctg tttgagcaac ctgatttaat
    67921 tgctgattga attcttttgc ttgttggatc ttcccgtcaa gatctgtttg gacagcttcc
    67981 gcttttgctt tcgcttcggc aagctttttc tcaagttgtg cacggtcatt tcccacttta
    68041 tttttaagat cagctaaatc ttgatcgatt gctgcttggt cttctttcaa tttgtttttt
    68101 gcagcttcta atgcttcttc gagttctttt cttttatttt ccaagtcttg caaatgatcg
    68161 atttggtcag aagaggtaga actatttatt gatgtatctt ctgaagatga aatcgtttct
    68221 cctgaattat ctgtcggtac attggaagtc atttctgttt ccgaggcacc gttttcagca
    68281 ccagaagagc ttatagttgt tgaaggttct gtttgagcag gttcagaagt ttccgcaaaa
    68341 gctgtctgtc ccaataagaa aatactggaa acagcaattg gccagattaa cggcttcatt
    68401 ctttttttca tcacttttcc catccttact actttttaac taatttctta ttataagcaa
    68461 aaatgtttgg aagaaccata aacacaacag gctgtttgtc taaaaaacgg tgcatctttt
    68521 atttttttgt acgttccggg cttttccatg tcccgcttcc ataacctaaa atcgttttga
    68581 ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac tatccagaac aaaagcatat
    68641 atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg atcatctaca atcagcgaag
    68701 ctagcaactg gaaaaatcct gcgaccattt caaagcagat gaacacaaaa ctgaatgtaa
    68761 aagtaatata aatctgttcg tagtttcctt gatatacata atgcccgatt aatccagcaa
    68821 aaagaactac ggaaatacaa aagaatattg accagacaat actcaatgtt tgatcaatga
    68881 agatgatcgt tcttccaata tgtttaaaag gatggagcat cacttttttg aaattcgtca
    68941 gccagacttc tgttccacct tttgcccaac gttttctttg tcgatatagc atcttcaacg
    69001 tcacaggtac ttccatgaaa aagataatct caggagcaaa aacagataac cagtcattta
    69061 actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg atcttgtctg aatccgccca
    69121 catcgatcaa ggcttctttt cgatacatcg tatttgctcc actgtacgca taaagaccac
    69181 cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat acttgagaat tcaaccgttt
    69241 gagattttgc aatcaatttg gtacgatttt ggacatccat atttgcagtg accgcagcaa
    69301 tatgtcttgc accagggcga atgaaataat tcacatacct aatcagtgca tctggctccg
    69361 gtaccgtgtc tgcatcattg cttaaaatca atttcccttt tgcaaaggca agtccgatat
    69421 taaacgcgtg ggcttttcct ttattttttt cgatccgaac cacacgaaga ttagcgtatt
    69481 ttttcatcag tctagctaat atttctggtg tttggtccgt tgaaccatcg tctgtcacta
    69541 atacctcata gttgtgataa ttcaatttcg tcatcaaata ctcgattgta tcttcgatca
    69601 caatttcttc attatgggct ggcaccatga tcgtgatgaa cggttcgaca ctcaagggaa
    69661 catctaccca ttctttttgc ttatgtttgt ataaaaacac ataacaccag acaccaataa
    69721 accatacaaa tccgcctaaa atcggataag aaacgagaaa aaatacataa tagacaaaca
    69781 agtatctaag aataaattac ctgtatgggt gattgagatc atttcagttc caccccattt
    69841 tctttataca aatctgcaat gaatgtcgta tctagatttt tctcttcgga gacagaataa
    69901 aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt ccgtatacac ttcttcaagt
    69961 aattcttgtc tttttttcaa tttttcatct tcatattcaa aagatttatt tacttttttg
    70021 tatagatttc gattgttcca cctagtcaat acaaatgagg taatcaggaa actgaaaaaa
    70081 atcaatacaa aaaagccgat taagaaatac agcattttct tttctgataa aaaagcgtac
    70141 aaggaaaaat ccttggctct ttctggtctc gttaatggga aaaagaccca tataaaagga
    70201 agaacaacga tcatccatcc taaaagagcc actaacgttt gccaaatctt taaccagtaa
    70261 ttaccttttg aaaaatactt atctttcacc aaaattgatg caacttcttc tcttgttact
    70321 tttttcatat cctcacctca cgtttttttg ttacgacgta ggagtttacc atacatttca
    70381 tttcttttca cataaagtgt tcctaaattt ttcctaagaa aaagaacaaa acatttctca
    70441 tcagcaaatg aaaaatattt tgttcttttt atcagttgtt ctttttattt tttatctctt
    70501 aattatgttg ctctttccaa tacgacaatg cttttcattc ctctagatat cgccgtgtac
    70561 atttgtctgc gtcttctttg ctcatctcca tcgttttcaa atggatataa gattacatga
    70621 tcaaattcca gtccttttgc tagactcaaa ggaatgattt ggcagttttc taatccatat
    70681 gaattttttt taatcgattc ttgtagcgtt tgggtttcct cttctgtttg ggtgataact
    70741 gccgcttttt ctccttttag agaaaccaaa atctcagcta gtattttcaa caattcttca
    70801 gaggatcgaa aagagcggta ttcgggttct tttccttgcg gtcggatcga tacgatgtcg
    70861 atcttctctt gatccacagc ataggttttg aaaagctctg tgattgctcc gctagaacgg
    70921 taactatttc taagatcata ccgatggatc ggcagatggt agtcgtcgaa acaacgcata
    70981 atatcagcaa aagtagtgga agaattaaag attgcctgat tctcgtcacc tactaaagta
    71041 aagtgagttt ttggaaataa ttcgatcaat agacctaatt gtgccagggt ataatcttgt
    71101 acttcatcaa tcaatagata gcgaaatgct ggtacattta ccttttcaac aagtaaatga
    71161 cgaatagcta atataatgac tgcttcatca acagtacgtg tgggttgcca agcataagct
    71221 gagccatatc gtttttcata aattctttca aacaactgat gctcatccac ccatgccatt
    71281 tcttcgattt gtctggttat ttttcgatat tttttttgta aaagtttcct tgcataagct
    71341 acaatacttt gttccgaatc atcagaaatc aatttaccga ataattccaa ctgcctatct
    71401 tctgtcaacg agagtacctg attctgcaca gcaggacttt ttgcttgttt caaaagatga
    71461 ttcttccatt cgctcatcag tgcctttttc gttgcttgaa gcctatcgat caatttagga
    71521 tgagcaggaa ctttctcata tattttttcg atcgtttttt tagaaaggac tgtttttcca
    71581 tttttagtaa gtccacggat aaaattcgga tgatctaaaa acaaaggatc tgattgtttt
    71641 agattatcga taaacttttt gctacgcaaa cgttctgtct gctccgatac attttctccc
    71701 gtgatacgtt tgaaataagc ctcttcacct tctatttcct ctgctgaaag ctgtgaaacc
    71761 agctgtatga tggttagatt cagtggattt ctttcaccta atgatggtag gacattcgaa
    71821 atatagtcga taaaccgatg attaggtgat agaatcagca agtcatcgga tgtcatcgta
    71881 tctcgatata aatagagcag atacgcaata cgctgcatga tagcagaggt tttaccactt
    71941 cctgctacgc catttaccaa gatattttga cttttcacat cccgaatgat tgtgttttgt
    72001 tccgattgga tcgtagcagt gatagccttc atttcattgg tctcatcttg gcctaagact
    72061 tctaataata cgtcatcttg aatagctaca gacgtatcaa aaaacttgac caaacgatct
    72121 ctttccaaaa gaaattgtcg tctttcttta ataaccgtat cgattttatt ttgacgaacc
    72181 gtataagaag atggaccaat catgttattg taaaaaagtt cggcaattgg tgaacgccaa
    72241 tcataaacaa gattttcatc tgcatcatct gtgaaatttg ctgtaccgat ataaaaggat
    72301 tcttttggct cattatccag aaagtctaca acgacttttc caaagtaggg acttttcaat
    72361 aaacgttcga tctttgctag ctgattttct gcgtttttct gtttgatatt catctgatcg
    72421 atttctctat ttttcatttc aatcatcgaa aaagtatcca ggttatctaa gtaactgtca
    72481 aaattcaatc gaagctcgcc agccatttct ttcaacgact ggtttccttc tatttttgcc
    72541 gtatccatcg attcccgcag ttcgtttttt ttatcaatta gcttttgata cacgttcttc
    72601 agatatgctt cttctaactg tttttgtgtg ttgaccatac acattctcct ctttttttaa
    72661 agtggtggaa ccgattagtt tacctctttt aagagtcgaa tgcaagcttt ttaacgagta
    72721 tcatttgtta ggatatatca gttcttcagg tcggacagtt tgatataaag aaaatgcacc
    72781 atcaaggttt tttacgtggt agccagactg cttcaagatt ctttccgcta gatagctacg
    72841 taatccgcta tgacagctga cgatatattc ttgttggctg tctaattcat ttaatcggtc
    72901 ccgaagttca ttcaaaggga tatgtttggc ttccttgaat ttgccctttt ccaattcttc
    72961 tgctgttcgc acatctaata aaatttttcc ttttgctagt tctgtcggca attcatgcca
    73021 ttgtacggtt tcactaagcc cttctgcaat gttcattgct gcatatccta gcatgtttac
    73081 tggatctttg gctgagccaa atggcggtgc atacgtcaat tctaattccg gtaaatcgaa
    73141 gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg atccgtttat ccacaccttt
    73201 tgccccaaca ccttgtgcac cataaatctc gccagtctct ggatggaaga tcaacttcag
    73261 taaaatatct gttgcttctg gataatagct tgcatgatct tttcctgata catgaatgac
    73321 ttcaaaagac agttcgtttt ctttggctgt tctttcactt aaaccggtgg atgcggcagt
    73381 cattccaaac gttcgtataa ttgctgttcc aataccgcct tgatttcttc gagaaattcc
    73441 ggaaatcgtg tccgcaactt gtctaccttg acgattggct ggtgaagcaa gagaaataag
    73501 tgcatctttt ccagtgattt gctgttttac aacgatagca tcccccaccg cataaatatc
    73561 aggaatattc gtttgataac gttcgtctac taggataccc ccgcgtatgc ctaattcgat
    73621 tccagcggtc actgctagct ggttatcagg acgaacacca atggataaaa taactaaatc
    73681 agcagggatg gtacgcccat catccagtcg caatcgtttc ccgtggtctt caaatccagc
    73741 gacagcatga gaagtaatca cttctactcc ttgatgagac aattcttttt ctataaaagc
    73801 tgccatttct tcatccagtg ttggaagaat atgtggtgct tgttcgatca ccatgacttc
    73861 taaacctctt ctttttaggt tttctgccat ttccaatccg atgaatcctg caccaacgat
    73921 cacggcacgc tttggctgtt tttctaatgc atgcatcact ttatcgatat ctggtacatt
    73981 tctaatagaa aaaacattgt cggcttctgt tatccctgga atcgatggaa caactggttt
    74041 tgcaccagga gataaaacca atgcatcgta ctgttcaatg tgtttttgac cctcatgtac
    74101 gacttctacc gttttgtttt cgggaaagat ggctgtcact tcatgatttg gacgcacatc
    74161 taaacaaaaa cgagcggata atgattctgg cgtttggaca agaagatttt cccgttcact
    74221 gatttctccc gaaagataat aaggcaagcc gcaatttgca aaagaaacat acggtccttt
    74281 ttcaaaaaca acgatttctg catcttccat taatcggcga agccgtgtag cagcagacat
    74341 tccacctgct acacctccga caataacgat tttcatctta tttccctcct actgttcgat
    74401 caaaccattg attcatgcca ccacgtacat tcactacatc ataaccagat ttctttaatt
    74461 ctttagctgc ttgtttactc ctcatccctg attgacaaat cacatagacg gtcttttctt
    74521 tgtctccatc atatctgttt atactttgta atggaacatt tttagctcct tttatatgcc
    74581 cgccccggta ttctgcaggt gttctaacat caagtaacgc tggattttcc aaaagctttt
    74641 cctttaattc tgggactgtt atcgatttta ttgattgaaa tagcaaattc attttctgca
    74701 cctcatttac atttttataa atacctcatg gggtatatta aacaggagct ttattaatat
    74761 gtcaaccaga atgactcttc atttttttca ctacttttta tatttgcata aaataccgat
    74821 aaggggtatt atctaattat aaaaatagag gagaatgtgt tatggagatt gataataaaa
    74881 atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg tggtatccaa aaaatgatcg
    74941 aagacgaaaa agaatgtatg gatgtcatca ctcaattaag cgctgttcgt tctagtattg
    75001 atcgcgtaat gggcatgatc gttgcagata atctgaaaaa ttgctttgag aaacctgata
    75061 gtaatccaga agaacaagcg gctaaactag aacaagcgat caagatgatt attaaaaagt
    75121 aaaggaatga tttcaatgaa agtcgttttc catattgatg aattagaaaa atggtcagaa
    75181 acagggaaaa acgtaaaaaa cctgataaaa gcttctccag aaacgacgat tgtcgtatct
    75241 gtaaatggca tcgcgattac cggttatcta gactctgcca atgcagaatt cttagatttg
    75301 caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg ctaatcatat ttcagaaagt
    75361 tctttacctg aacaagtaat cgttgtacca gcaggtgtcc ttgatttagt tgaactgcaa
    75421 tcccaaggtt atgcttatat caagccttag tcagtagagg ttgcgacaga agtgatcagc
    75481 ttcaagaaat ttcggcttat ttttcgaaga agctacttct ggaaaaccgt gaatcaaagg
    75541 ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat
    75601 atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa
    75661 gaggttgtga aatcagcggt ttgacctaag tattagagaa aaaaacggga aaatagctcc
    75721 tcatattttg ccgttttttg agctaatacc gaaggtcagc tgattgaaca ccgtttaagg
    75781 aagagattgt gaaaaagtcg cttagtttcg agtattaaga aaaattttgt gaaaatggat
    75841 ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga ataccgttta
    75901 aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagaaacaaa cggaaaaata
    75961 gctgctcata ttttgccgtt ttttgagcta ataccgaagg tcagctgatt gaacaccgtt
    76021 taccaagagg ttgtgagcct accatttaac tccgaatcac aagaaataat ttcaaaaaca
    76081 acttctcctg tttttggttt ggttggctta ttatcgagaa gtagctactg ggagtctatt
    76141 tatcaaaaaa aggaatgcga cataggtcac accccttttt tattttcttt gaaacatctt
    76201 tcggtactcg acagcataca agacaaaaga ttgtaaaaga ccgtaagtga tgacggcaaa
    76261 aatgactctc acccacagcg ttggtaattc tgggaaaagc agcaaaagga tcatgccaac
    76321 atagatgaca gcagtcgcta ctttgccata ccacttcgct ccacttaatt tgatattttt
    76381 agatagcccg attaatccag ctacaaacat aaagccttct tttatcaaga atagaccaaa
    76441 caacagccaa agccacggtc gataccatgc catagacaaa atcagaaaaa gttgtgtcaa
    76501 tttatctgca aacggatcaa gcaccttccc taagtctgtt acctgattat atctccgagc
    76561 aatgaccccg tctagataat cagacaaacc tgagacaaca atgaccgctc ctgctatata
    76621 aaatgactgt ctcagcacaa aataaagata aatcggcagc aaaaaaatac ggaatatcga
    76681 taaaagattc ggaatcgttt gtatttcttt acgccagtct gtgcttaatt tcattttatc
    76741 gccctacctc ctcagcactc ttttcttttt attttagctt acacggggaa taaaaccaat
    76801 gatttcactg tttttatttt tctttacagc cactgatatt atgagctaat ttgagataat
    76861 cacttgctgt tttcatcgtt tactcttctt tcttcctgtc gtttgcgcat ttcttttact
    76921 agtactttat gagctttttt tgatcctcgc tctaggtcac gctgccgttt acgtgtttta
    76981 taatcgataa acagactgtc taaatcgtaa cctgtcgggt acagatcttc tgctgaacgc
    77041 accagacgaa cacgctgacg aggcacgtgg attatttttt tgtctaaata tacatcaatc
    77101 gtatcttcac ctatatcttc ataaactaac gcttcttttt ggtattcatt gacaaatatc
    77161 cgatcacctt ttgcaaagag ctggcgttct tttttattct ccttcctatt tgtaactgct
    77221 gtcttaggaa gaaattcttt tttctgtgtc cgataggctt tttccgccaa gtaacgctct
    77281 gcttgatgga tcaatttcat cgacatatcc attttatgag caatccacaa agcttggctt
    77341 tctcctgttt ctccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    77401 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    77461 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    77521 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    77581 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    77641 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    77701 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    77761 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    77821 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    77881 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    77941 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    78001 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    78061 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    78121 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    78181 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    78241 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    78301 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    78361 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    78421 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    78481 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    78541 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    78601 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    78661 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    78721 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    78781 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    78841 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    78901 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    78961 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    79021 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    79081 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    79141 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    79201 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    79261 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    79321 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    79381 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    79441 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    79501 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    79561 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    79621 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    79681 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    79741 catctccaag caaaactagg atcagtgtcc caataaactc tcccatgatt tgagtcatat
    79801 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    79861 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    79921 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    79981 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    80041 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    80101 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    80161 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    80221 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    80281 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    80341 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    80401 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    80461 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    80521 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    80581 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    80641 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    80701 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    80761 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    80821 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    80881 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    80941 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    81001 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    81061 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    81121 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    81181 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    81241 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    81301 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    81361 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    81421 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    81481 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    81541 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    81601 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    81661 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    81721 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    81781 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    81841 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    81901 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    81961 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    82021 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    82081 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    82141 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    82201 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    82261 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    82321 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    82381 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    82441 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    82501 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    82561 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    82621 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    82681 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    82741 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    82801 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    82861 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    82921 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    82981 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    83041 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    83101 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    83161 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    83221 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    83281 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    83341 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    83401 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    83461 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    83521 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    83581 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    83641 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    83701 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    83761 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    83821 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    83881 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    83941 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    84001 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    84061 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    84121 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    84181 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    84241 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    84301 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    84361 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    84421 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    84481 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    84541 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    84601 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    84661 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    84721 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    84781 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    84841 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    84901 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    84961 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    85021 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    85081 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    85141 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    85201 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    85261 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    85321 gcgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    85381 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    85441 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    85501 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    85561 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    85621 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    85681 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    85741 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    85801 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    85861 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    85921 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    85981 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    86041 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    86101 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    86161 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    86221 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    86281 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    86341 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    86401 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    86461 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    86521 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    86581 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    86641 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    86701 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    86761 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    86821 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    86881 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    86941 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    87001 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    87061 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    87121 atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    87181 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    87241 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    87301 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    87361 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    87421 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    87481 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    87541 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    87601 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    87661 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    87721 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    87781 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    87841 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    87901 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    87961 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    88021 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    88081 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    88141 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    88201 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    88261 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    88321 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    88381 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    88441 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    88501 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    88561 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    88621 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    88681 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    88741 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    88801 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    88861 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    88921 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    88981 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    89041 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    89101 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    89161 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    89221 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    89281 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    89341 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    89401 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    89461 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgtttcaa atagtcaatg
    89521 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    89581 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    89641 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    89701 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    89761 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    89821 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    89881 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    89941 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    90001 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    90061 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    90121 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    90181 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    90241 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    90301 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    90361 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    90421 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    90481 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    90541 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    90601 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    90661 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    90721 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    90781 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    90841 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    90901 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    90961 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    91021 ttggaaaaac tgttgaaact tccccgttta caggactgta gatttttccg tccgttggaa
    91081 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    91141 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    91201 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    91261 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    91321 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    91381 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    91441 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    91501 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    91561 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    91621 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    91681 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    91741 ttgcataatc tgcaattgga cctacaacta ataaagtaat tgggaccatc acgatgatac
    91801 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    91861 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    91921 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    91981 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    92041 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    92101 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    92161 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    92221 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    92281 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    92341 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    92401 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    92461 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    92521 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    92581 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtacccctta
    92641 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    92701 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    92761 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    92821 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    92881 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    92941 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    93001 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    93061 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    93121 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    93181 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    93241 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    93301 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    93361 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    93421 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    93481 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    93541 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    93601 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    93661 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    93721 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    93781 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    93841 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggca
    93901 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    93961 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    94021 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    94081 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    94141 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    94201 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    94261 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    94321 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    94381 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    94441 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    94501 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    94561 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    94621 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    94681 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    94741 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    94801 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    94861 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    94921 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    94981 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    95041 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    95101 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    95161 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    95221 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    95281 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    95341 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    95401 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    95461 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    95521 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    95581 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    95641 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    95701 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    95761 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    95821 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    95881 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    95941 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    96001 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    96061 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    96121 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    96181 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    96241 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    96301 tggaacagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    96361 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    96421 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    96481 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    96541 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    96601 cgtcctagca ttgtctcttc ctttcgcatt gattgattcc aagatttcct cttttgtaaa
    96661 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    96721 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    96781 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    96841 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    96901 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    96961 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    97021 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    97081 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    97141 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    97201 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    97261 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    97321 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
    97381 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    97441 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    97501 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    97561 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    97621 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    97681 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    97741 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    97801 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    97861 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    97921 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    97981 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    98041 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat atgaggaact attttttcgt
    98101 ttttttctct aatactcagg ccagaacact gatttcacaa cctctttata aacggtgttc
    98161 actcagctgt ccctcggtat tagctcaaaa aacggcaaaa tataagaaac tattttttcg
    98221 tttttttctc taatactcag gccagaacgc tgatttcaca acctctttat ttcacattca
    98281 atatccacat gaatcccaca aatacaaaga acagaccata catgattgga gatacttctt
    98341 ttcctctttt agctgctaac atggtaattg gatagaagat aaaacctaat gcaatcccat
    98401 ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct
    98461 ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat caatactcct accataatca
    98521 atgctggtgc agttacttgt gaagtaacaa ctgatagtaa tggagaaaag aacaagccta
    98581 acaagaagaa aatccctgta gtaatagctg tgatacctga acgtcctcca acggcgatac
    98641 cagctgatga ttctacaaat gcaccaacag gagaagtacc aaataaggaa cccgcaagca
    98701 tagctgttga atcagaagct aaagctttcc ctacacgagg catcttgttg tctttcatga
    98761 aacctgcttg gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca
    98821 agaatgtcag cactacgacc cacatttgaa gagaattgat gtcccctaca tgttttaatg
    98881 caactaaaaa tgttggtttc aaacttggtg ctgcggcaac aatttcagaa ggcattggaa
    98941 tcaaacctgt gaccagacct aaaatcgttg tagctgctat cccgatgaag atgccacctg
    99001 gtacacgccg aacaagcaag atagcagtta cgactaatcc aaaaatcgtc aaccaagtag
    99061 aaccaacgtt gagtgatcct aatcctacta atgtagattc attcgaaacg atgatcccac
    99121 cttcgctcag accaaggaaa gcgataaata gaccgatacc gccagaaatc gcatatttca
    99181 aatctgctgg gatcgcatca atgatcattt cccgcagctt gaagattgtg atcaaaataa
    99241 aaatcaaaga agcaacgaat acgccggcta atgctgtttc ccatggaatc cccattccta
    99301 aacaaacaga ataagcaaaa aaagcattga tcccaagagc aggtgccgtt ccaattggat
    99361 aacgggctaa tacccccatt aaaacacagc cgatcgcact ggctaatgca gtcgcagtaa
    99421 acactgctcc ttcatccatt ccagaagcac ctaatacagt tggatttaca aataagatat
    99481 aagccattga gataaatgtt gtaaatcctg ccagtacctc acgttttacc gtggtgttca
    99541 atttttctat ttcgaagtag gaaagtattt tttccttcat cttccatccc cctaaatccg
    99601 aacattataa ataaagtccg ttaaaatttc gatttattaa taaaacaacg tgttttatca
    99661 tatactaaaa caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt
    99721 ccatctgatt aaatcccttt atctcaaagt tcatgaacat tcactttgga ttacaagaat
    99781 ttctttattg tgttaataaa gtttcttcat tctagtctca aaaatcaata gtgtgatttg
    99841 attttaccat ttcgtttctg ttcttctttg atgaattctt ttgtatcgac aggattcaga
    99901 ttatttagtc cgccaaaacc tgtatcgacc atttttcccc agccttttgt cttctttttt
    99961 cctgatttga gattctttgc tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa
   100021 taaccttcta atcttcccat tagatccact gtttttcctc ctttttattc aatcagacga
   100081 ctcagctttt cctataacaa aataattgcc ttcagggtca gaaaaattga aagtcaagat
   100141 gccattgtta tctgtgattt ctcctggttc ttccatgttc tcgtagatag tttcaaattc
   100201 atccgtaaaa aatactaaag aaggcggtgg tcctaatacc tctggtgaaa acttctgcac
   100261 aaatactttt gggaatacgc ctaattctaa ttcttttgag atgctgagta caatactctt
   100321 aaattcttct ggcaattcga ttgtttcaga aatttctgca ccaatttttt ctacaaagaa
   100381 tcggcaaatc atctctacgt cattgacata aagcatcaca cgtgtttttt gtatcatcat
   100441 ttgacccttc tttctatttg attttctttc ctgtctataa actgattcca ttatatcacg
   100501 aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa taaagcaata gtttctagga
   100561 ttgctttcct ccacaaactt tttttacaaa aaaaaaaaac ggcaatgaat gccgctcttc
   100621 ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt gtgtctaaca
   100681 ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc ctgtgcgcca
   100741 cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg tgtttcttcc
   100801 gtcagcattg ttctttgggc ttcacgccag ttgagacagc gacagatcgc tccttgttca
   100861 tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg
   100921 aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct
   100981 tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc tacaagcgga
   101041 ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc aatagaagaa
   101101 cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc aatgacttcg
   101161 ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat
   101221 aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc atttacggat
   101281 aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac tattactttt
   101341 ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag tctgtttggg
   101401 gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt ttacgataaa
   101461 aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt ttttgctttt
   101521 cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga tgtttaaatt
   101581 tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc tcagattgac
   101641 tcgttcctgt atttagtttc aggtaacccg caggctcttg atcgatactc actaggaaaa
   101701 aatcggtttc tttatctttt agctcttctg taagtttttc tagtgcataa gcttgtgtca
   101761 agtaatcagc taaatgctcc ggtgaattat cttttccaaa tgtatctgta aatgtttcga
   101821 tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt tcgtatttat
   101881 tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa ttccaatctt
   101941 cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct tcttcgtcag
   102001 aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg aaaggataga
   102061 tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct ttatcttcca
   102121 aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc gttgtacgat
   102181 ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg caaatacgta
   102241 ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata ttgctaatag
   102301 aatctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt tcatcccacc
   102361 tttattttgt ttgtaagact agtttacctc tttttgttgc aaatgcaact attttcaata
   102421 aaaaaatcaa gttctttttt aataactagt aaagtatatt cttttcttct tttacgaaaa
   102481 agctcaatca caatagatct ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt
   102541 tcgcttttct gatagctttg gcaactcttc tatactaaaa aatcccatgt tggaagtttc
   102601 attattctca ataaacttgg cctcttcacc gtggatcgca caagcaaaaa caagtttata
   102661 atactgaaaa agctgtggaa tatctttttg tttattcgta tcaaaaactg ctcgcaactg
   102721 agtagtttct actgttaatc ctgtttcttc atagacttct ttacgcacat tttcttcagg
   102781 agacaatcct atttcagcaa atccaccagg aagtgaccat tctttcgtcc gtttatcttc
   102841 gattagtagg accttgccat cttttttgat ataggcacga acatctactt ttggtgttgg
   102901 atgtccttct ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt ttcctatttc
   102961 actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat caaatacatc
   103021 ttttccataa tatagaccag cttgagaaag tgcgagtagt cttttgtatt gagccaactt
   103081 cttgttcatt ctcttccctt cttttttagc aaatgtatga ctatgtaatc aagtatagaa
   103141 gactggttta attacggcaa ataaatataa tgaaatctgc aataaataaa agaatgttaa
   103201 aaaactcgag aacaaaagac attcgacttc tctggggctg tgaataaatg gcgttcaaaa
   103261 gcttgcatct gcggtaataa gccccaccaa acgaaaaata tgacgagcta ttcttgtctg
   103321 gctgttctta ttaattgagg caggacagct ttttcacaac ctccccacta aacggtgttc
   103381 tcctaccaac tcctcggtca caagtcacaa tattcatcaa acatgagaag ctgttttttt
   103441 gaaattgctc cttgtgactc ggagctgaac gacaggctca cgacctctcg gtaaacagcg
   103501 ttcaaaagcc aatttttcga tattaagcca tttctttgtt acttattgtc attgatttct
   103561 ttttctaatt tatccaatag tggagtgtaa tccacttgag tatagtctgt tacatcaaat
   103621 tcatatagtg ttcctaactg gaatttgtca tattttcggt cggcgatgat ctttttgaat
   103681 gcttctttcg taatatgatt ggtccctttc gttcgatctt ccaatacgtc accatagtga
   103741 taatgatcca tgatatagct caaatgacga tctgattcac gatcacgttt gtaccgacgt
   103801 tgacataaca cttcaaagtc tgctgttagg cgaatcgtga tcacctgata tccgtatcta
   103861 tctgctaatt cttggagtcg tgctttttgc ttatcactaa aaggatactc ggacacaatc
   103921 actttctttc ctgcttccat atacaaatca agtgctttgt aatagaaagg ataaactttc
   103981 gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc cgatggattc tgcatagtat
   104041 tccttgattt catcgagcgt gatggcatac atatctggaa atcgttcccg tagtttattc
   104101 ataagatatg tttttcctgt accaggacta ccagccagaa gaatgaaata ctttttcatc
   104161 tcatttattt cctaagttaa ttggactttc caagttcata aacagtccaa tcttatgctt
   104221 gacaatttct tttactgctt catttgctgg aatcacatta tggcgaagag acttgttgat
   104281 ttctgtttca ctaaaggttt ctttagctac tttcgtccag ttgactaaaa gctccgttcc
   104341 aacattgaat ttgcggatcc cgttattgat caattcagga taatcttctt ctttcacacc
   104401 tgttcctcca tggataacta gggggacatc aactactgca tcgatttctt tcagcaaggg
   104461 aatgtttact tctgtttttg atttgaattg tccatgattc gtaccgatag caatagctaa
   104521 tgcatccacg cctgtagctt ctacaaattt cactgcgtct tccggtcttg tatatacctt
   104581 gtcattttca gcgacatgga ttccttcttc tgtaccgccg attgttccaa gttctccttc
   104641 tacggaaaca ccgtgcgcat gggcatattc tactactgct tttgtcttca ggatattttc
   104701 tttgaaaggc agatgggacc catcatacat gacagaagta tatccgctgt cgattgcttc
   104761 tttgatatca tcaaagtctc gggcgtggtc taagtgcaag actgcatcaa ccatttcttc
   104821 ttctgcaata gctgtcatta cattgactaa cacatgcatg ccgatatatt tagcggtatc
   104881 gacacttgtt tgaataatga tcggcgaacc catttctttt gctgcgccta gcatagcagg
   104941 aagcatttct aggttatgcg tattaaaagc tccaactgta aaattcaact cttctgctgt
   105001 ttttgttact tctttcaatg ttgtatacat cttttattgc caccttatct ttttatttgt
   105061 ttcctttgat cgttacttgt tttgcaattt gtcgcatctc atccattttg cccatgtagt
   105121 aatcgatccc ttgatcatca ccagcacgag cagcatctgc acgcttctgg ttatagaggc
   105181 tcatcagttt tttataccca tctccgatcg tctgtttatt ttccaaactc ttttctaaat
   105241 attggatggc ttgatcgttc acatacaatt cagaaaattt aagtccaact tgttcataaa
   105301 gtttcgctaa tttatgctta tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct
   105361 ctgattgaat cttttcggtc gcttctggcg atagggcttt ttcttctttt gccagaactt
   105421 ttttcggttc tttttctttt ttcttaaaaa atccaaacat cattttccct actttcaatt
   105481 aaaatgcttt caaaactggg cttaagatcc aggcatataa gagtgctgcg gctactgtat
   105541 ctacatcggt tgctgtagca ttgatgaatc ccatcgaatt aaagatcgtt actagcaaag
   105601 cgggcaataa tgtaataaag aaaccatgag caataccgcc aatgatcgct cctcggcgtc
   105661 cgccaactgc gttgccaaat atccctgctg tccctccggc aaagaaattg gttagcatac
   105721 caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa
   105781 tcgttgtcgt aatgaaacct aaaatgacag catttgggct ataaggaaag aaaactggac
   105841 aatctaaggc agggatcgca tcaggaacta atttcatagc tatccctcga aatgccggca
   105901 cgatttcacc taacagtaga cgtacaccag ctagcaatac atacacaccc actacgaatt
   105961 ggatggcttg taaaaaggca aacatgatat agttttgatc tcctgataat tcagaagcga
   106021 atggttcgcc tgcaaataaa acagtgatga tatatagcgg gaccatcacg accatgacag
   106081 acaaataagt atcttgcaga aattcgaaat gtgtcggcaa cttgatatct tcgatcgact
   106141 ttttattttc gcctttctca ccaaaaagct tagcaacacc ggcttcaaat aagtacccga
   106201 tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt gattttacga atgatcggtt
   106261 gcgctactgc cggcatagca atggcgaaac aagctccgat aaaaccaccg accaagatca
   106321 atacgatccc tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt gccatccata
   106381 aaattgcttg accagttaaa aaaatgtact tccattttgt gaaacgagcc aaaataatat
   106441 tgaatacaaa aatggctaag aacgtcagtg caatatctct acctaaaccc aaatcattca
   106501 ttgcttgacc attgatagat tcaatagaag ggatgatccc ttgcatatga aatccttcag
   106561 taaagatttt cccgaaataa gttaaacttc caacgatgat gctcgaaccg gcactcaaca
   106621 cttgaaatcc taggagtgtc tttaacgtgc cagagatgac ttgtcctgct gatttcttct
   106681 gcagtagtag acccaacata gcaatcaatg caatggtgat cgatgcctga gtcaaaatat
   106741 tttcgatgat aaaattgata acgcccatgt ttttaccctc ttttcttttt gtatttagat
   106801 aactccctta tcttgcagaa ctggggtgat tttttcttca atttcatttt ttgaaacaat
   106861 atttttcaaa tagatcatcg ccgttttagg atcaactgcg aacttttcga actgcgaacg
   106921 aaaattttct gctgtgatga tcacatctgg tttcatggaa actgcggaag aaatatcaca
   106981 atggtccagt ttggcttcta ctcctaattt gttcaaaaca tcctcagcag acatctgtgc
   107041 tgcaaaacta cttcctaaac ctgcaccgca tacgaataaa atgttcaatt tgttcatctt
   107101 tgttctcccc cgtatcattt attatttctc aactactgaa aaaagcagtt gtttgaaatc
   107161 ttcgatattt gtgcattcaa tcaatcgatc caacttctct tcgttgttga tcaattcaat
   107221 tagttctttc atgatgttca gatgcgagta ggagtctact gctgccaaac aaaaaataat
   107281 ctttactgga tccatttcag gattaccgaa atcgattggt tgctcaatcg tagctacgct
   107341 gactcctaat ttattgaccc catcttctgg tcgggcatgg gctagtgcca tgtgtttgcc
   107401 aatcacgata tacggtccgt aatcattcac tgcacgaatc atcgcttcga catagcgctc
   107461 ttcgacaatg ttttctttaa ccaatggtct agcagctagt tcaatcgatt cctcccaagt
   107521 agtggctttt tctttgatca tgatattgct gtccgtcaaa atatctttta acataggctg
   107581 gatctccctt ttgttgattt ctaaattgtt tgtttcgaat aatttttcta acttgccata
   107641 gatgattcga tctactcggc caccactttc tttcatgaca tctattattt gagtgaataa
   107701 ctccgtcgat tcatgactgt taggctctaa ccgctgtagt tcttcatttc ttttcaaaaa
   107761 gtcttctata accaagatat tcgtctcagt taaaatcgga tcgacaacta aaagcggctt
   107821 atttcgataa ttcaattgaa aggtgctgaa aaccagattg acatccaatt tatctactaa
   107881 ttcgatttct tttgaactta aaactgcagt aatctcgatc tgaggaaact tttctttcag
   107941 attctctgct aacagatttc cagtagccat cccatggtta cagacaacga ctgatttata
   108001 aacataattc aagtctcgct tgattacact ggcgatcgtc gaaaagtgga tgacaagaaa
   108061 agctatctcg tcctccagta tcttgccacc aattacttct tcgatcgtag gaacaaaatg
   108121 agtgactgcg cggtagattg ggaaataatt tgttcggatg ttttctttca atggattcat
   108181 gacttggata tgattcttga ttcttacaat cagtgcagcc atgtgcttat agagattttc
   108241 gaataaagct tcttccttta gatggaaagg tacgtgtgtc tcgttctcta caaattggat
   108301 caattggatc gttagtaatt gagcttgtac ccactcgatt gaattggaga tatctctagc
   108361 attgaacgtc tcaatagtga aacgaagata atttctttct acttgcggga cattgtccaa
   108421 ttgaaattcc tcgatcatgt ggtcaataaa tgcagaaaag ctactgtctt ttatttctgt
   108481 atttttccaa ttaatggcag agataaattc atgtttcctg attcgctgga tccagatcaa
   108541 tgtaaaaagc aggatttgtt ttttataaat atcatcttct tgtttcaaca acttattgga
   108601 atagatctcc tgtatttttt tgatttccac caaaggaaaa taatggagaa agattttttg
   108661 agccggtgtt aattgtgcat cttgattcat aaagaagtcg gtcattccca gattctgatt
   108721 gataaaatca tagatcatcg tacggataga tcgttctgaa cctttataaa ccagtccttg
   108781 ctttccataa ctgacgatct ctatcccata actgaccagt gttgctcgta accgtctcat
   108841 atcttcatct aatgtacttt tagagatttg aaattcttct tcttttttgt ttagatggat
   108901 cgggattcgg ctgtaagcaa cggctaaaac tagatcaaaa atacgttcgc gccgattcaa
   108961 aaagccgtct ttcacatctt ttatttcagt ttctaatcga gcttgctttt ccttttccag
   109021 cttcaacaaa aaaactttgt tgcgaattgt acggatctcc ggaagctttt tactgctcaa
   109081 ccagatattc gcttcaagaa cttcctgacg gatcgtccgc tgactgacat tgaactcact
   109141 tgctagatgt tttgctttta ctggatattg ggctgtcatg attctgtgaa gtatttcaat
   109201 tgttcttgtt ttcatagaaa tgatctcctt ctgatttcaa gtatatggtt ccgcttacat
   109261 aataaaaagt agatatttct gccacttgaa gtggaaagaa agtaagcaaa gcaaacaaaa
   109321 aataagccat atgctttttc atctcctcaa cgaaaaagac atacgcctat tattacaaaa
   109381 attttgccca tctatacaaa aaagaggttg tgaaaaagct gtcctgcatc aagtaataag
   109441 aacaactaaa ctaaaatagt gcttcctatt tttcgtttgg ttgttcttat tatcgcagat
   109501 gcaagctttt aaacaccatt tagtggagag gttgtgacag agatgttcag ccttgagcga
   109561 taagaagcga ttttgaaaaa tcgcttccca tattttacaa aatcgtgcct tattgccgat
   109621 aggctatctc tggcacactg tttattcaga aaaaggagct gcgacaaggc ttttgtcaca
   109681 actccgttca taaatatttc ctgtcaatcc agcataatcg gctgaataaa atcggcttgc
   109741 catctttttt ctttctcaga aaaatcaaaa taccatatct gagaaaaata ctgtcgttgt
   109801 gtcttacttc ctgagatcaa tcgcccatct tttcgatcga ctgaataatc acagcatctg
   109861 aaatcaattc ggatggcaaa actattttcg tggatctgtc gataatttcc tacttcctga
   109921 atagttattt ttttagtatg atttcttaca ccgtctgctt tgtttttttg cagaactttc
   109981 tgatgttcgg caaacagctt gtctgtataa cagcttctgg ctttttcagg agtttcttct
   110041 tcccaagcgg attggatctt catgaatatc ttttggattt ccagaaactg tgctttcttt
   110101 tctttagccg ttcctgacat gcgggacaat atctccgctt tcagcctttt cttttgtctt
   110161 ttattcttga cgatagaaaa agcagaaacc cctcctactg acaaaacagc taatgcaaga
   110221 aatcccgttt gctcataact agaaggacct gtacctggac cataataccc ataagaatct
   110281 ctgccgtaag aacgactgcc aaaataactg cccccatatg aacgaccacc aaatgaacct
   110341 ctgcccgaac ttcgaccgct agatgtacct ccaccatgac cacccgcgac agcgaatcct
   110401 tgggtcggca gagcaaacaa acataggccc actaacaaaa tgattgctaa ataattgatt
   110461 tttttcattg tcttcctcgt tatcttttat tacaaaaaac tatttttatg atagccttgc
   110521 taaaaggaag agtcaataac cagatgcata aactgttttt ttagcaattt tgtcttattt
   110581 agtcattgag taacatcgga caaatgtatg tgtcggcagt ccataattcc tctatcttca
   110641 gcatgaacaa gcttgcagac aagagatctg cgcttcctcc aggagagatt tggttttcct
   110701 gacaaaagtg caacagtgct gtaaatggac gatttggtaa agcactttga ctagcagcat
   110761 gagcaagttt ttgcatttcc actaacgttt tctgatcact gcgatgcaat acgcatgtgt
   110821 catctagcga actcatcaaa actaacaaca tatgcatttg tttctgtaca tatgaatcat
   110881 tatctagctc cttagctgct tggattgcaa gcctgatatg cggataccct aatatcgctt
   110941 cttcatatgc accatttacg ccatattttt gcttcaccct atgaccatga gtggtttttt
   111001 tatgacgata ttttgtatca ggaaaagcag caagcttgct gacatcatca agtattttcg
   111061 ataaagaaaa aggctgttcc atactgattt tttgtgcaat gacactgatc aatagcccca
   111121 ttgcccaaat cgctccttta tgggtattga tgccatttgt agcaatcagc attgcctttt
   111181 ccgcttttcg cccaatctca gcaattcttt ctcttaactc ctgatcaata gcatgatgcc
   111241 atgctatttg agccatttct tcaaaaaaag gagttagcgt cttagcagac ttaagaaata
   111301 aagcatagtc catatcggaa tgtgcacccg catccagtgc atccactaat ccaggttttg
   111361 gacttaatgt tacttctgcg atcaatgctt cctctaccaa cgcagcgagt tgctgagtca
   111421 ctgctttttt tcgttcgtca tttatcatga cactcactcc ttcttcctac tattacttgc
   111481 tgaaaccttt tttgatagct acccaaatga tatgaagtaa ccagcttaaa gcaaatgcac
   111541 aacttgccgt cattagataa gctacaagaa tcatgactcc tgaaggtaac tgcagccaac
   111601 tatctttaaa caataataac agtacttgga aaacaaatac attggctaaa tacgtacgat
   111661 aagcataaac agacaaccat ttgaacaatg gcaattttgg cgaatcgttt acaatcattt
   111721 tggaagcgcc tatgaagaca gcaaagatga tgactaaact gtataaactt tggatcgtat
   111781 tcaagtaagg tgcattcgca aagctcaagt ctcctggatg tgcgagcaat gaacggacag
   111841 aaaagaacgc gataactaag gcaacaggta agaaaagata acgtactctg tccaaatatt
   111901 gatagacttt ttcgtgataa acaaatgcgg ctaccccata aatcatgaaa ctagcaacaa
   111961 accgatctaa taagtaccag ctgtcatgat aaggcccagt caatacttgt gtttgataga
   112021 acagatacca tgcgacatat aaaatcgttg aaccaatcaa tactggccat acatattttt
   112081 tattagcaaa tactttgtaa ccgagccaaa caaaaaacgg catgaacaat tggatctgca
   112141 gcatcataac ggtataccat aaatgaggcg ctccatcacc tgtgacatat ttcagtaaaa
   112201 agcttgcagt atccgtatac ggagtttgtt gttggactgc tggaaatagc caaagataag
   112261 ccgtcgtcca tagaagatac ggaacgacta gttcaaaaaa cttttctttc aaaaattccc
   112321 aataagacaa atgctgactt gtttttacca tattataaac aattgcaaaa ataaacattg
   112381 gtgctgtgta tttaacgatg acgtaaaagg taccaagaaa agctgtattt gttgcgctgt
   112441 ggctgtttgc caaagcaaag ctcaatatcg tttgcatcat aacagccgtt acagctgata
   112501 ctttctggaa gtcacttacg ttgattttga tttttttgtt ttcaaacatc tctttctctc
   112561 tctttttctt tattaccaaa gttctttggt taatttaggt ccttcgactg ttttcaatag
   112621 aatttttttc tctggctctc tcaaatactc ttttagtgaa aaaccgcctt gccgagtcac
   112681 tacttgggta tcgaattgct caaaaaaagc aggatgcgat tgaattacct gaattggcaa
   112741 ttcaatcggt gaatttgcgt acagcagcaa atcaaaatca ctggtttctt ttactgccgg
   112801 aatcccagtc gataattcaa atcctaagct gccgccgatt ccccatttac atcctgataa
   112861 tatgtttcta gcagcatgaa attgctgata aacaggataa tccatgattt tctcttgtct
   112921 atacgattct tgttcgatga tgtcccacgg atgaatgact tgtttgacgg catgttttga
   112981 gatttctgtt gaccatctct gattgcgggc aggtccacgt aaaccaatcg gtagtatttc
   113041 tgcgtgatcc gtcgacgcac gacgaactgt tccgaacatc ttttcagtta gaacgaccca
   113101 ttcaggcaag gtgaaccctt ctatttcatg tttatcaaaa aggacaatat catgtgcttg
   113161 aatcattttt tatgcatccc attcttcgtc cattttggca cggactttat ttgtttcgac
   113221 acgtccgaat gttttggctg tttcgtttgt gtatcgattt gtcagcatcg tgtcatttgt
   113281 attgcggaca tcttgaatgg cttcattgat tgcttcgatg actttgctga cattttgttc
   113341 tgaagcagct tggtcggtga tatcttctag gaaatgatag atcgctccta gttttgtata
   113401 attattgata tcgtaagcga tagctggaac tttttcagca gctgcttcga tttcagcaac
   113461 cgaacgtttt gtcacacgtg cagcactttc tttagacatc gcttggacgg taatttcttt
   113521 attgtttaaa gcaatcaagc ggcttgattg taatccatgt gctaaaaatg cacctgaaat
   113581 agcatttcct gcaatcaatc caataactgg atgtccagct tgacggagtt ttgcataagc
   113641 agcagcacta ttagccaaag ccacatggat accgatcaat tcttcgttat agccgtatgc
   113701 ttggcttggt acatcgatca caacgatgat tggccgtttc acttttttgt ctttatcttc
   113761 ttcgtaaacg tgatccaaaa tttgtcccat gcggaaacct tcaactaaac caacttcacc
   113821 gtttcgtaca cggtacatcg ggtttttagg atcaggtacg attgatgtca cgatggcttc
   113881 ttctatgaat ttacttgatt taccataata cacagtacta atagaagaag ttggattttt
   113941 catgccagtt agtgccgcaa accaatcgta accaacactt gctgcagctg gttcatttcc
   114001 ttctgctact tctggtaagt caattgattt tgcttgatgg ttagcaattg cttcattgta
   114061 atcgtcaatg gccattggtt ttgaaagatc taaagaatca agtaatgata aataaaatgc
   114121 tgagctttca ctgcgatgcg tgtcttttcg ttcgtccatt gctgcgatga cagcagcttt
   114181 gattgcttct acactatcag tcactaattc atcaatgatt tttgattcta cccgttgacg
   114241 agatcctaat gtattccaaa tcaagtcttt gtcactagaa tcaaattctc gaacgcctgc
   114301 ttcttgttca ataacttctg gtccattcaa accgactctc gctttttttg ttgcgattaa
   114361 ataactcatt aatgctgaag taatcgacat accgccaaat gatcctacac gtcctggaat
   114421 caaaccgatg actggtacat attgttttag agcaatgatt acatttccga tttctgaaat
   114481 agataacagt ccgtagtttg cttcttgcaa acgaacacca cctgtatcta gtacaatgac
   114541 tggaaagatc tcatttcctt ttttattttc ttctaacaca tgttcaagtg cagcaacgat
   114601 ttttgcaccg gaaacttcac cgataccgcc accttggaat tttccttcca tagcgattac
   114661 tactgcttgt ttgccattga tcgtgccacg gctgacaacg atcccgtcat cactttcagg
   114721 gacgattcct tgtgctggta aacttggcgc gatgatttga tcgaatggat caatcaattc
   114781 acgtccattg tcaagtagag cgttcactcg ttctctggcg tttaattcta caaaactatt
   114841 tttcatctga aagttcctcc attgcttgcg tcaatcgtag gtacacaact cctggtgtag
   114901 caccgaagtc attgatcacg atatctgctg aaattggata acgatcaaaa aatctcgtta
   114961 atacattgtt ccatacttcg ccgaagccat cgcttcctgt acaaattttt acagtagatt
   115021 ctgttttttc tgttggaaac atcaagattt ccaaatctcc tgaaccgacc actcctacat
   115081 ggattttctt attgatcggc tgtttcgttg gatatttata agctaattgt tccatggatc
   115141 tctccccttt ttgtaaaaca gagcatacac gttctgctta ttcgtctttc ttcttccatc
   115201 tgggtaaatg gcgacaaaag gtttttctcg ccaattttat gaccaagttc tgaatttcat
   115261 tggtggttca tacaggccat ctgaccattc gatcaaatca tcgatggttt gtgcagctaa
   115321 taatgaacgt ttcgcttcac tacgtttgat gcctagatct tctggtaatt tcacgatgcc
   115381 ggctgcacgc aagctatcta gtgattcttt cgtgctagtc attccaagag gtgtaacacc
   115441 gccaattgct gcaattgctg cttgtcgttc ttcttttgtt tttgctttgt acaaataagc
   115501 aattccttct tctgtcacga tatgtgtagt atcttcgcta taaatcatca ctggtgcatt
   115561 ggctaagcct gcttgttttt gaacagctag tgcatctaat tcttcgacaa agactggttt
   115621 tttatttgaa ccatatgttt caaccatttg aacaactaat ttttggcctt tccctaatgg
   115681 atcatcattg tcgcgaagtg attgccatgc tggtgtagaa tgacgacgac cgccaggatt
   115741 atgtcccatg ttaggcgcac cgccaaaacc agataaacgt ccttttgtta cggtagaaga
   115801 gtttccttcg taatctaatt gcaaggttga accgataaac atatcgatgg catattgtcc
   115861 tgcagcttgt gataatgttc ggttactgcg catcgtgccg tctttaccga caaaaaagac
   115921 atctgggcga gcagcaatat atttttccat accaacttcg ccaccaaaac tatggatgct
   115981 ttcaacccaa cctgattcga tggctggaat catcgttgga tgtgggttca atacccagtt
   116041 cttcgcgatt ttccctttta accctagtga ttcaccataa gttggcagta acaattcgat
   116101 agctgctgta ttgaatccaa taccgtgatt caaagattgt acgccgtgtt ctgcgtagat
   116161 tcctttgatt gccatcattg ccattagaat ttgcaattct gtgatgtttt gaggatctcg
   116221 tgtgaataaa gcttctaatt gataaggttt atctgctttc acgacaacgt ctacccagtc
   116281 accagggata tctacacgtg gtaaagaatc gacaacttcg ttgacttgaa cgatcacgat
   116341 accatctttg aaagctgcag cttcgatgat tgttggtgtt tcttccgtat tgaaacctgt
   116401 gtacaagttg ccgtctttat ctgctttatc tgcagctact aaaacaacat ttggaatcaa
   116461 atcgatgaat aagcgtccat aaagttccaa ataagtatgg atatcaccta atttcattgt
   116521 gccgtcttca atcatttggg cgacacgcaa gctttgtgca ccagcataag agaaatcgat
   116581 cttgcttgcg attccttttt caaaaatatt caaatgttct ggtcgtgaga tactagacat
   116641 gatcaaatgc agatcattca ctttttctgc atctactttt tcaagacttt ctgaaagaaa
   116701 acttgcttgt ttttgattgt cgccttctaa tacaactttg tcgccgggag caatcagttt
   116761 ttccattact tctacgattt tttcagtttc tacatacttg ccattgagca agttgctgat
   116821 tttttccaat cgctgcgctt ttttttcgcg tttagaagcc cagttgcggt ttggtttttc
   116881 cataattagt catctccatt tttgatatag aaacgtggcg tcatcaatac catgttggtc
   116941 tagattgatc gtacggatcc ctttatctac accaaatttt gtatgaatca gctttgtcaa
   117001 agtattgccg gggggaaatt caatcgtcag atccattcct aattcttttg ccaagtccat
   117061 catttggttc cactgcacag ggaaagctag gttttccaac aaatctttac gaatctcttc
   117121 tgcgtcctga gtggctcttc catccgtatt ttttatatac aaacatctag catcagatag
   117181 atggacagac tgcatatacg gcttgaattt cttgaccgta tcattcatca agagacagtg
   117241 agatggcaca gggactttca gcagcttcgc tgtttgcgct tggtgccgtt tggcttctgc
   117301 caacgtaacg gataaacctt cgatactccc agataacgta tgttgcatcg gacaattttc
   117361 atttgacaga taaactggat ggtcaggatc aaatgttttt gctacaattt cctcagcttc
   117421 gcttctcgtc acacctacta ctacgcccat tgcataaccg cttggataag cttctttcat
   117481 taagactgcg cggtgataga ctagttccgc tgcattctca aatctcaaag atcgacaagc
   117541 aacagctgca caaaaagcac caatcgaatg tcctgctgca atatcaggct gaataccgtt
   117601 tttttccatt tcatctaaat aaaatactgc ttttgtcagt aatgctagct ggataaaaac
   117661 cgtatcttga tagttttctt ctttatcaac taaccgatac cccgtcacat tttctaaacg
   117721 ttccatgtac atttcaggga tatccgctaa catccctaac ttctgactgc cttgtccagg
   117781 aaataagaaa cctgtactca tgagcctcgc ctcctcagca cttacttttt ccactgctat
   117841 cttagtcaat ttctgctata ataaaaaatg ccaaaaaaag aaaaaaccat aacaaaaagg
   117901 tatggatggg atggataatt atgaaattgc ttcatctgca gtattttaaa aaagtattcg
   117961 aaacaaaaaa tgttacccaa gcagccaaag aattatttat ttctcaacct gccttgagcc
   118021 gtgcgatcaa acatttggaa aacgaattag gtgtcccttt gttttaccat agcggcagaa
   118081 acattgagac gaccgtttat gcagaagaat tttatccata tgcagtcaag acattagaca
   118141 cattagaaga aggaatcaaa ttattgtctt cggtcaatga gaagatcgtt acttccgtca
   118201 tattgtattt agaggtagcc tctgtttcta ttccgaactt ggtccgaatt tttatggaaa
   118261 agcatcctga tatccaatta acgatcatgc aacatcattt accagatgat attaccgatc
   118321 ctgtactgta catcacttcg gagatgaagc ctggcatgac caatattcct atcatgaaag
   118381 aacctgttta tgtcgccatt cctaagaaac atcctcttgc taaaaaagaa catctgcaat
   118441 tagaagatat ccaacgcacc cccatcttga tgctgtcaaa gagcaacgcc ttccgtcata
   118501 cactggatgc agcacttgaa gcgaaaagta tcgaattgac gatcagttcg acgacagatg
   118561 atcctgcgac attacgttct attgtcaagc aaggattagc tgttagcttt ttcccaaaag
   118621 tttcttggtc ttacgataaa aatgatcctt tcgtcttgcg ggaaatcaaa gatttgccct
   118681 tgacacgaac aatttattta tcttctagct tcacagaaga tcatcctttg atcaaaatga
   118741 ttgcaaaaac gatgcgggaa ttttatttag gaaaataaaa aaaaaagagc tgggacaaaa
   118801 ttcttgtcgc agctcttttt tcgaacaaac ggtgttccag aagtaatttc ttagaaaata
   118861 agccgaaatt tcccaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
   118921 gctaaacatt tctgtcacaa cctcttctct attgtcctag acattcattg cgttgattta
   118981 aaacttccat caatcaaatc tgctagttca tctagctttt ctattttacg tagatcaata
   119041 gagatccaat gttctttatt catgtgatag gctggaaaat acccctcttt tcctcgcaaa
   119101 attccgccaa gttcaggatc gaccttcaaa tcaagaatat tgatgatcct atggtcactt
   119161 gcatcatcat agattttttc ttctggaaca gacatcagga ccccaaacca ttttttgctt
   119221 ttttgatgtc tgaatacgca ataatcagga aattttttaa aaatatattc tggagacaaa
   119281 ttgtatgttt cattgatgtg atcgattagt tcttttttcg ttagcatctg cttccatctc
   119341 tttcttattg attcgcttta ttcaaagata ccacgtaaga atagaaaaat gaatacaagt
   119401 agttgctaat caaatacttc gatatttttc ttttttatca actcttaaat gttcaatccg
   119461 cctgtgttgg tcttgctacc atctactatt aaaatgttta ttattgtttg cccacctatt
   119521 ttttgatttt agtggtatca tttttcaagt tcatgatgaa attcttatca tcagtaaatg
   119581 ctccagaaaa ataaacaatg attgaaaaat cagttgaaac ttgaccaccg tttattttct
   119641 ccgtattctt agaatagaag ggagtctgat tgatgtattc actaatgaaa aaaatcatta
   119701 ccgaaaaaga tattcgacgt catgtttctt tagtagaaca attgctgaat catacaaaga
   119761 taaccgtaaa tgaattagct gaaatcatag ggacaaccga acgaacgatt ttttctgacc
   119821 ttcaatctat tcgctctcag ctgcccgaag gctgggacat catcagcgat caagcaggaa
   119881 tcagtttgca aaaccagcaa aatttattaa caaatgacct ttgggagatt tttttcaaac
   119941 aatcagtcag tgtcgaactg ctgaaaaatc tgctttttac aaaaaaagtt gctgtccctg
   120001 attttctagc cgaccatggg ctttcttatg ggacattgaa aagacatgtg actaaaatca
   120061 atcagcgttt agcaagttac gacttgcaga tcgatttgac gaaatataca gcttgtattc
   120121 ttggaaaaga gcgtgcgatt cgtacttttt atcatcgatt gctgattcct ttcactcata
   120181 acaactattt tttcgaggat tattcgatcc atgaatccca ttattttcaa tttttaagaa
   120241 acctcagtca aactgaacta gcggttgaaa cagaagagat tttcggaaca tgttggttct
   120301 ttatcaatac gatccgtata aaagcgaatt gccgattaga ttcttctatc cataccaaca
   120361 gcacactctc ctccctgtat gactctgcat tgaaaaaatt gtatttaacg gaaggtattt
   120421 atcttaaaga cactgagctt tcttttgctt ctttctgctt tctggaaagt tggaattaca
   120481 acaataacta tggacaagaa accgctcgct gcctgcatca ttctccattt ttagaggtcc
   120541 tcgaaacgtt tgtagaggaa ctagcttcag agctgtctct tgatcaacta aaaaaaacat
   120601 ctctgactga taacttggct ctcctcatat taaaatatca tgaatcgccg atattgattg
   120661 aagcattgga ccgtcaatac catcgttttt tagaaacata tgatcagcaa tatgcacact
   120721 tgtatcaaca gaaagagaat ttactgaacg atctaaaaaa agaaatagaa atcaatgaac
   120781 caaattactt tctccaactc ttgtctctac tgatccaaca aacgattttt tctatcaaac
   120841 cgaatttgtt caatgtctat tttattttcc aaggagaacc ttcatggaag gcgttcttgc
   120901 agcaggaatt aaaagattac ttaggaaaac gtgtctgctt cttacctatc gaactaaccg
   120961 atctttccac tatttctttt gaaaaaacag atatcttgat ttccaatttc cctctagatt
   121021 ctctagatat ccctattgta tacatttctt ctattccaac gaaaaatgag ctcaatcgtt
   121081 tgactgaact cactttcaaa acttttttat agtaacggct agcgactgta ttctctagct
   121141 gttttttctt ttttgcgaaa cagtttgttc atgatccctt tttctatttc tacaactaag
   121201 aatagaagaa tcacatttgc tccaatcaat aactgttgtg tcatcgttaa acttactgta
   121261 ccgatcagct gctggccgac tggtagaaaa ataaccatcc cttgcaataa agcaaggata
   121321 cctaatgaaa gaaaaagcgc tttgttttgc aaaagacctt ggttcaatga cggcttggac
   121381 aactctctgc aattgatcat gtaaacagct tgagctaaga cgatattttg cagaagaatg
   121441 gtttgctgta agctttgccc ttcaaactgc atagccaata aataggcagg aaccatgatc
   121501 agtagggaga catagatgat ccgaaacaaa tgataaccag ataggattcc ttgctttgga
   121561 tctcttggtg ggcgcttcat gatttctgga tctggcgact cgaaccctag tgcataagat
   121621 aaagtgattg tcgttaccat attgacccat aaaatctgta ctggggacaa aggtagcggg
   121681 cgattcagca taagtgccca aatcaagatc aatccttgag ctaatgcagt tggcaagaag
   121741 aaattgatgg ttttttgcaa attatccaaa atccgccggc cctcttttac ggcttttgca
   121801 atcgtatgga aattatcatc tcctaaaacc atatcagctg cctgtttgct tacttcacta
   121861 cctttgattc ccatagcaat tcctacatcg gatcttttca aagcaggtgc atcatttacg
   121921 ccgtctccgg tcattccgac gatttctcca tttttctgca aagctttaac aattcgcagt
   121981 ttatgttctg gtgtcgttcg agcaaagaca tcgactattg gcacttgctg aatcaactct
   122041 tcatcagaca taagatccaa atcgatccct tctaaaactt tggcagtatg ttttaagcca
   122101 atttgttccg caatcgcttt tgctgtgtct ttgtgatccc ctgtaatcat tttcactgag
   122161 atccctgctt gttggcattc ctttacagct ttgactgcac tttcttttgg cggatcgatc
   122221 aatccggcga tccctgcaaa agttaattgg gttaaagtct catgcgtgag ttcctgtttc
   122281 gaatctactt ttttataagc aaatcctaaa acacgctgac cttttctcgc aaattctgca
   122341 gctgtctgac tccatgcttc ttgttctgca gaagaaagag ttgatttagc aaataatact
   122401 tctggtgcac ctttgacgta aatcaccgct cctttttcct ccgcatgcat cgttgccata
   122461 tatttgtatt cagaactgaa cggaatcttt gcttctattt ttttcaaagg tagattggct
   122521 gtttctgtgt gtcgaatcag cgcaacttct gtcggattgc cacttaaatt ttctgttttc
   122581 tgttcttctt ttgtttttaa ttcctgacag tttttcataa tggacaacac gcaggcttct
   122641 tcttttgcag ctacatccac aactgtcatt tcattttttg tcaatgttcc tgtcttatct
   122701 gaacagatca ctgtcataga acctaatgtt tctaccgaag gaagtccttt gatgatcgcc
   122761 ttttcttttg ccatttcatg tactccaacg gatagaatca tggtcaaaac agctggcaat
   122821 ccttcgggta ccattgacac caccaaagca atcgtagccg aaagaaggat atgccaatcc
   122881 ataccatatc gaaatgtcgt aaaaataact aagaataggc tcaaacaaag aatgccttga
   122941 aaaatttgtt tgtttaatcg gttgattttt ttaatcagag gcgtcgtttg ttgttcgacc
   123001 gattgcaatg cttgattgat tttaccgatt tctgtcgtgt ttcctgtttc tacaacgaca
   123061 ccgactgcag aaccagattg tacaagcgtt cctgaaaaag ccatattttt ctggtcgccg
   123121 atcgattctt cttctgtcaa aacggcatga cttttttcag atgcttctga ttctcccgtc
   123181 aaaatcgatt cttccactaa taaatcatgt gcttcgatta atcggatatc tgctggaacg
   123241 acatctccgg cacgtaagac aatgacatct ccttttacca gttctttcgc agggattttt
   123301 tcttgcatcc cgtttgatag tactatcgct tcttgtccca tcagctgctt caaaccattc
   123361 aatgattctt ccgcttttct ttcttgccag taactaacga gtccattgac gatcactacg
   123421 aataaaataa tacttccttc aatatattca ctagaagcaa atttcaaaaa agaggcaatg
   123481 atcaacacga tcatcagcag atcagtaaaa tgctttgcta tttttttcca tcgttttgtc
   123541 tgctgccttg cttttatttc attatatcct tgatcagtca ggcgtttctg tcgttcttct
   123601 tgcgacaagc cttctgttga tgtttgatag tttttcaata catcttctac cgtcaatctg
   123661 taccagttca tcctatcccc tcctgtttaa ttgatactgt tattatacgc agggaagttt
   123721 tttcaggcgt atcgattatt tcaggggggt ctgaaataat tcgttttgca aaatgtatct
   123781 gggcctaact gatatttcta ttgcttattc ttttatatac aaaatttaag aatacaaacc
   123841 agataaccaa taaaaaagga ataaagcgtg tcgtgctttc gaataacaag ctgaaagcaa
   123901 ttgaagtaaa aaacaccaac aaagcaagat ctttatatct ttgtgtagtg ttccgtttct
   123961 tttctttaat aaattgaaca tgtgcacaca agatcgaaga ccacacaaat agaaaacaac
   124021 tcgttgtcat gcctgccaaa agctgaaaaa acaatcgact atcagctaca aaaaaatgta
   124081 gacaaagacc ggcaaaaaat aacattcctg taattaaaat accgtgtaaa ggtacgcctt
   124141 tcttagaaag cgcacaaaac caatggggca aacttttttc ttttgctgtt tgatataaaa
   124201 tcctgctagt actgtagact gcactatttg ctgaggacaa agaagcaacc aaaatcatta
   124261 gattgacaat tgctgctccg ccttggcaat ggatcacttg cagcatagtc actaaaggac
   124321 tttcttttaa atctaaacaa tgccatggaa caacaaataa aatcgacaaa atcgttccta
   124381 tatagaaaaa gagaatacgc caaggcagac agttgattgc ttttttcaaa ttcttttgcg
   124441 gatcacttgt ctcgccaacg gttaagccga tcatttcaat tccagcaaaa ctgaataaaa
   124501 ccataggaaa tgagactacc attccagcaa agccaaacgg tagtaattca tcgatctgtc
   124561 caaaattttc tttgattccg atagactggg tatgaaaatc tgagacactc aatcctatac
   124621 ccaataagat aaaaaggaaa atccccaaga tcttgatgat tgagaaaaaa gattccaatt
   124681 caccaaacca cttgactgca acaaggttga ctgccattaa gatcattagt gcgctacctc
   124741 cgcttatcaa agctggtaca tgtacaaacc agaaatggat atacaatcct attgctgtta
   124801 attccgcaag tccagctcca agccagcaaa gccaataagt ccaattgaca aaaaatgccc
   124861 actttttgcc taaataatag tcaacgaaat cactgataga gttaaatgta ctatttgcta
   124921 aaaataaatc acccatggcc cgcatcaaac cataaatagc caaaccgcat atcaaataaa
   124981 cgagaataac agctggccct gctgatgcaa ttgcctttgc tgaacctaaa aataatcctg
   125041 ttccaattgc gccacctaat gcgattaact ggatatttcg attagttaga gtccgtttca
   125101 ttgccggttt ttccatcaag ttcacctttt tctttttcaa gcagcatggc gacttcctgt
   125161 tcccaagaag gtttctttat tttttcaata cagagtggta ctacaaacaa aatgacactg
   125221 atggctgtga ctgcaattag attgatccat gttccagctt tcatcgtcag tccgacagaa
   125281 aatccgattg ccgcaaataa aacaataacg acagtccaaa ctccaagatt gctctttttc
   125341 tctttttttc caattctaaa tggtcgattc aactttgcat gtgtataacg caatctcaaa
   125401 actgcgatcc ccatgatgac atagacgata cagtagataa cagtagttga atttacgagc
   125461 atcaaaaatg cttgattgac gccaggaatc agcaaataaa aaagtgcaaa gattgagata
   125521 atgacagact gagtcaaaat aacggatttg gatacgtcaa attgattcgt cttccaaaaa
   125581 ttgaattttg gtggatacgc accttttcta gcactttctg tcactgtctt tgctggtcct
   125641 gaggcccaag cagataattg gactgctaca ccaatgaaga caagtaaact aaacagattc
   125701 acaataagat gtggcaaacc aagaatttgg caataaatcg aaatagattg cgcgatatta
   125761 ttcaattcca tttgtccttt tggtacgaca tttgctacaa ctaaagcatt caaaatattg
   125821 acgaaaaata taaaaattaa cgcggcaaat actccgcgaa tatacgtttt aactggattt
   125881 tccaatcgag tgatatacac agaggacatt tcgataccag taaaaataaa catgatcggc
   125941 gcaaaataaa caaatgagga tgcagtcgta gtatctggta ttaatttgtc ccatgaaaaa
   126001 ctgcctaatg ttccggttgg tacgatccca actttgatcc acgcagcaaa ccctaataag
   126061 agcatcatcg tcaaaggcac gtacaatcct aaccaaacac cgacttttcc gccgattttt
   126121 gccatatcaa atttcaaatt caaaagagtg atgatccaat aaacgactaa aatgacaact
   126181 aatgtaaatt tgctgttcaa accaagcggc acattatcga taatattacc aaacaacgga
   126241 gcgagtgctg aagcgaccat caccatccca ggaaacattt ggacccataa cagccatgag
   126301 acaacgaacc cccatttttt tcctagagca ttcgataccc aaagttctgg accacctttt
   126361 tggggaaaca ttccagcaaa ttcaccagag atcaatgaga ttggaaaagc gtacaataat
   126421 actgcaacca gcatgtaaaa aatcatcgtc cagctcgttg ctgctacatc tggaatattt
   126481 cgtacacttg ctacaagggc gcaggtcatt ccgataaagg tcatggtcgt gatttgcttt
   126541 gatgaaccag tatctttcat ctttattcct tctttcaata gcgacatatg tttttgtttt
   126601 cttttatttt tgttcaagaa cagaaatgac aaggtttcat cgattcagta caaaacccag
   126661 tatcaccgaa acatccattg aacgatctta gtgaactggg tttgatacat gaaacgaacg
   126721 atccaactta tagaacgttt tataaaaaag aatatcttgg cgaggaatat atgaaaaaca
   126781 gggggaaata caattgtttt gatgaagcaa ataattgtct gttctccata tgtctcttgt
   126841 tattctttca cacaaaaatc agttctattg gatgatttcg tttagtttct tttccattgc
   126901 tttggcgatt ttttttgtat aaaaattgaa aattcgatca tcattcaaat aaggagtcat
   126961 gatagccgca cgtagcagag tgattttctg ttcttttttc cattcttctt tcgttagacc
   127021 cattctttca acaaaacgaa tcggagaatc accgtaatct tcttgtgtaa atgtagtatg
   127081 agaagtaata aaccgttcac cataaacatc gccatccata taagaagacc gatcgaacat
   127141 tttttcattt aattcattga tagcgttcaa atctgtacag ccttgttctt tgaataccca
   127201 attgaccata ttaaaatcag gatgattcaa tggatagact tcgattgttt ttcctttaac
   127261 agtaaaagtc agatgatcga gaaattctct gaatctttga gctgcctcga ttgaagcccc
   127321 aatcaattga ccatatccgg tcacattcaa tggcaataca cgatgtgctg tccagactgc
   127381 ggcggcggtt gctccagctt ttgatccttc caaaatataa gctcctaaca tatctggagc
   127441 ctttactgat ttttcaaaca catatggcgc gaaataagaa atgatatttc tcatattttt
   127501 atgtttgatt acgattcctc cagcagcata agggacatag cccatcttat gaggatctat
   127561 cgtgacagaa tccgcttctg aaatagctct gaacccttca tatacctctt tgtcaatcgt
   127621 tacgcagtgg tggaaaacat gatgttcttc aaagaattca gctaaggaag catacggcac
   127681 aaattctcct gcttcgttta gaaacagtga acgcgcatag ccgccatatg cggcatcaac
   127741 atgtaaatag aaatagatcc cttcatcttt taacctttct cttaattgga cgattttatc
   127801 tacactgtct acttgacctt cttctgtcgt tcctacgaca gcaaccacac ctagaattgg
   127861 gattttttgt ccagctaatt cacgaattgt tttttccaaa gcattaatat ccattcgata
   127921 atcctcttga actgggatag caaccatctg atctaagccg actccgcaaa tatctaacgc
   127981 cttcatccaa gaatagtgtt ttgtttgagg gaccagccat ttccctaatc tttggatatt
   128041 ttttccgcta cgggaagaag ctgctttgac ctcatccatt tcttcgtccg taaagctttc
   128101 ggtcatttcc aaaatttctt caacagataa attcaaaagt tcccactcag acattttttt
   128161 cactttttca ggcagcactt ccttgacagc taaaggaatc gatttgatgc aacgggcgta
   128221 ccataatcct tcaagattag caatagaacc atctgctgca atgtgtcccc atccgtctgt
   128281 catattgaat aatgaagcaa aatcctgtcc tacttctgct tccatttgcg aagttgccat
   128341 agaagattcc aatgcaacat tatttgggtt ccacagcatc gcatagttgt aagctaataa
   128401 agcaggcatc aatgtttcag cattcatttg tccccagtaa cggcctgctg aatgccatgg
   128461 gatcgatcct gcgcgtaatc gctgactgac ttcatccaag acctctatca tatggtcgcg
   128521 ggtggctaga taatctggag tgagcttatc tccttcgcta attgcaggca tatctgaagg
   128581 aatataattt tctctccatc caagatgttc atctaccatc ttgttcaaca acattttata
   128641 aactggtcca ttttcagctt tgtcgcctat aaagacggcc ttgatatcca tatctttcat
   128701 ttttcttccc tccatatttt gttggtcaat gtcttgcaga tacatgtgtt tatctgtaac
   128761 acctcttcac tatagcgcca aaaatgagaa attttttttt ataacatcaa atccacgtag
   128821 atttttacaa acaaaaataa ataaagtcgg atttttctga taattcaacg caaaaaagcg
   128881 aacaattttt caataaatca gtcttctaaa atagataacc taaaaaaagc gaacaaacaa
   128941 ataaaatgac tgtccttcat tcactaaaca tatttttatt tttttatatt gttaaaaatt
   129001 cacaatttat tttttaatag aattatctgt tttccaaatt ttataacata aaaaaccttt
   129061 tttaacggca tttatatgct ttttcagaaa ttaactgatg atttcaaaaa gaaaacatgt
   129121 cataatattt cccatattgt gcaaaaaaat acaaggcgga ggctctacta tgattgaaga
   129181 atatattgag aaaaatattt taagacagct gtttttatgt gggcaattct atgtaaataa
   129241 agaagtcaat ctagaaaagc tctccaatct cctgcatgtt tgcaaaacca ctttactcaa
   129301 tgatatcaat aacataaaaa aagaatttga agaacagatc gcttatacac atcgggaaaa
   129361 ggaccgttac actttatatt tttcagaaca tatacctcga tgcaagatca tgcagcaatt
   129421 gtctcaaaat tcgttgtttt taaaaacttg tctgctttat ttggaagaag atgaacctga
   129481 ttatcttcaa ttaactgaat gtgaattcat ctctgtcagc aaagcttatt cattaaaaaa
   129541 acaagtatta gcttacttta atgactgcgg gatcgaaatc gaccgctact cccctcgatt
   129601 cactgaaatg gaaagacgtc tgctgcttct taatgtgtcg tatcgtttag gcggttttaa
   129661 ttcttgggag ctacctgaat cgttttttga acgtgccgat cgtttcattg aatccgtaac
   129721 ggaaaatagt ggcagatttt atgataaaga aaataaagaa atcttatcga ttggttttgc
   129781 tatcagtttc ctacgccagc aagtgtgtgc tgtgacaata gattcaaaat ttatcgaaga
   129841 aatcaaaaaa cgtcctattt acaattatgt cgaatcagca tgggaaaaca ctgatttcca
   129901 aacctattat aaaaaagaag aattcgcctt tattttgaca ctattcaatc tttgtaatta
   129961 tggtttccat tcttatcagt tgattgcgga agactttcaa cagctacacc aagtgtttat
   130021 tgacaatact cccgaaataa aagaactagt tgctaccttt gaatctcact tcaatcaaga
   130081 attattcgga aaccaaccgt tcgaacgtgc gctgattcat ctgatgcgtt cggcttggga
   130141 taactatcaa ttattcatgc cagaaaaatt ttatctttta aatgaggaac agacaaattt
   130201 atataaagaa gtccagacaa ttttttccag ctggtccagc cagttacctt atgatctacg
   130261 gttgaatcct aattgcatgc gggcttttgt gatcgaattg agcggaattc tccgtctgac
   130321 aaaagaacat ctgactatct atatcgtcac aaattcggat gttcattatc tgatttatcg
   130381 ggaagccttg gaagctgtaa ctactttcga ttttcaagtt gcaccaacaa tttattcaag
   130441 tatatctgat ataaagaagt atgcgcagca gtcctcaaac cgggtattat gtgaacgtac
   130501 attatataca ccagatgcgg tccaatatga aaatattatt cctatttcga tcaatacgat
   130561 cgatcgagct atcatttcag ctgtgcaaaa caaataaggg gctgtgacaa aaatcatgtc
   130621 acagcctctt atttgttgaa gatgcccatt tctgtaacaa tctcttgata cacgtcaggc
   130681 tcacaacctc acttattttg gacatctagg ggtccgatct ctttatttaa aaaataagaa
   130741 atgatggttc ggataactac caacgccgct agttggaaaa tatcctgcaa ggtaggtttg
   130801 ataatcgatt cgatgatccc agctgaaaca agaatttcca aacttagaag tatatagcct
   130861 cccaacatct ttttaatagc attgttctcg cttactcggt cgctttgcac ttttgtatgg
   130921 acaaataaat atttcaaaaa atccttgatt gatagacaaa caccccaaac caataaacag
   130981 atagcaaaga tatcaagtcc cagtacaagt ttttcgaaat atggaagaag aaactgcatc
   131041 aactctgtcg tcatcctacc tctcctcttc tttgtttttc tttgtcttct atttcctgat
   131101 tcaagaaata agaaatgact gttcgaatga taataataag tgcaagcttc aaaatatctg
   131161 tccaagtcgg cttgataact gactcgatga tatcagcaac gactagtact tccaagctta
   131221 acaaaatata acttgctagc agttttcttg tatctgcatt acgagtatta cgttctgcat
   131281 agtttttctt gacatgagac caatcaaaaa gtttgaatag aacaagaacg atcccgacaa
   131341 ttaagatacc gatagaacaa atatttaaaa tagcaatgat ccattctaga aattcaatta
   131401 aatttgagcg aaacatatac gctacctact ttcttttaat ttgttcctat tcttttattg
   131461 taaaagaaag ttaccttgtt cgtctattta aaagttgttc taatgataac ggttcgctta
   131521 aatttttctt tttcactctc ccatttttct ccctccatcc ataaatcgtg gaagtatcca
   131581 aaacaacgta gcaagttaaa ataaggctta gtccgtcatc agcttttcaa agaagcactg
   131641 tctcggtgct ttttttgtac ccaaatttct aaaaatagaa ccatacacct cctcaaatct
   131701 tttcttctta cctaccctat ttttatttac aggaaaatca gacacttctt cttttttgtc
   131761 aaaatttcct ttttcttctc gtcctatcat ttggcattct aataaaaacg aactatcctt
   131821 ggatatagaa agagaaaaca aggaggcgtt tttcatgagt aaaaaattaa tcaaagtcgg
   131881 catcggttta ggtttgcttg cccttggcgc tgcatatcta ggaaaaaaga caggactatt
   131941 cgaagatgac agtcatttat atgatgaatt cgaatcaatc tagcatccta tttagttgaa
   132001 tcactacgga catacttgga agacattgga ggaatcacaa tgagaaaaag taaagcaata
   132061 atgattggtg ccggtattgc caatatggca gcggctgttt atttgattca agaaggcaaa
   132121 tggaaagggg atcagatcac cttttattca ttggatgatc acggttcaaa tgatggtgca
   132181 ccgaccgaca ctgtagtgga tgaatattgg aataaaaacc atccattaga aaaccaaaaa
   132241 ggctatattg ctcgaggcgg tcgcatgctt aattaccgta cctatgtaga tttgatggat
   132301 ctgttaagtc gaataccttc tgttactgag ccagggatga ctgctgaaga agatacgaga
   132361 gattttgatg caaaacaccg aacctttgac aaagcacgtt tgatggaagg tggcaaagga
   132421 atcattgatg gcggaaaact gggattcaat aataaagacc gtattcttct gactaaactg
   132481 attgctatgc ctgattcaga agaagaaaaa ctagacaata tcactatcga agaatatttc
   132541 aaagatgatc cacacttttt tgaaacaaac ttctggttta tgtgggaaac aacatttgct
   132601 ttcagaacac gtagttctgc tcaagaattg cgtcgctata tgcatcaaat gatttatgaa
   132661 ttcactcaaa tcgaacatct agtgggcgtc aatcgaacac gttacaatca atacgaaagt
   132721 attatgcttc ctttgatcga gtacttgaaa gaacaaggat gcaaaatcat tttgaaccgc
   132781 cgtgtcactg actgggaatt caaagaaaca ccgatgcaag atgaaatcac tgtgactggt
   132841 ttgaaaatta ccaatacact gactcaagaa gaagaatacg ttgtggtcga tgaagataca
   132901 gcagttattt tcactaatgg gtccattaca gattcagcta ctttaggaga ttacgaaaca
   132961 ccagctgtag aaaatatgga ttatggtgca gcttctagtc tctggaaaaa agccagtgaa
   133021 cgtttctaca acttgggaaa tcctgacaaa ttctttgctg atcgagatgc aagcgaatgg
   133081 gtcagcttta ccctgacgac gaaaaaccat ttgttgttga atgaaatcac ccgtatcacg
   133141 actcaagaac caggaaatgc cttgaattca tttatttcaa ccacaccgat cacgccgtta
   133201 ggccaaaaag atgtcaatat gtctattgtc gtgcatcatc aaccgcattt tacgtctcag
   133261 aaaccaaatg aaacagttat ttggggttat ttcctttatc caagacgacg tggcgaattt
   133321 gtggataaac agtatattaa aatgaatgga aaagaaatgc tagaagaact gattggacaa
   133381 ctttcaaaag tagatcctgg accagtgaat attcgtgaaa aagaaacaga aattttcgac
   133441 agcgtaataa ataatatccc tgtctatatg ccttatgctt ccgcgttgtt caataatcgt
   133501 gccaaaagcg atcgaccaaa agtcattcct aaacattcga ccaatctggc ctttacagga
   133561 gaatttgtcg agcagcctta tcagatgatc tttactgaac aaagtgctgt acgttctgga
   133621 gaaatcgctg ctttccattt tgcgggtatt cctatgtccc gtcttgtcaa aacaccgcgt
   133681 tacgacaaag atattcctac tctagctcgt gccgcaaaaa aaatgtttga ataatcaaga
   133741 ggttgtgaaa aagctgccct gcatcaagta ataagaacaa ccaggctaaa acagcttctt
   133801 atgtttttga tttggttgga cttattaccg cagatgcaag cttttgaaca ccgtttacca
   133861 agaggttatg aaaaagctgc cctgcatcaa gtaataagag cagccaagct aaaacagctt
   133921 cttatgtttt tgacttggtt agacttatta ccgcagatgc aagcttttga acaccgttta
   133981 ccaagaggtt atgaaaaagg cgtttagttt cgagtatgaa gaaaaatttt gtgaaaatgg
   134041 ttcctcacgt ttttgcataa atttgactta ataccgagaa actgatccct tctgtcataa
   134101 cctcttttta tttatgtctg tttctttttg aaaacaacta ttttttcaac gtttactctc
   134161 ttgccaatcc aacatcatct aacggataca actatttcaa tgattaccat tacactaaaa
   134221 aatcggaaac acacctctcc gaacacaaat acataagact atagaatgtt tcatttaaga
   134281 aaaaaagcgt ttcattatca aaaaggtaag atctccttta aaatgaaatt atctgtagta
   134341 ctaataatgc agacaatttt atgaatctga aaggagcatc tccaatgaaa gcagtcactt
   134401 ggcaaggaaa acagaaaatg gaaatcagaa acgtagatga tccaaaaata ttagaaccaa
   134461 cagatgcaat catccgtatc acagcaacag ccatttgcgg ctccgatctg catctttacc
   134521 atcatggtga agcagttttg gaaaaagggt atgttgtagg acacgaacca atgggaatcg
   134581 ttgaagaagt gggatctcaa gttcaaacat tgaaaaaagg gcaacgtgtg gttatccctt
   134641 ttaatattag ttgtggtcat tgtcatttct gcctgaatca catggaaagc caatgtgatg
   134701 aatcaaatcc agatcagtta tatggcgggc ttttcggatt tggcaagttg aacggtaatc
   134761 attggggtgg tcaagcggaa tatctaagag ttccttatgc agactctact tcatttgttg
   134821 taccagataa tgatcttcct gatgaaaaag ttttatttct atcagatatt ttgcccacag
   134881 cttattggag cgttgaaaac gcaggagtaa aaaaaggcga tacagttgtg attctaggtt
   134941 ctggtccagt cggtctattc gctcaaaaat tcgctgttat ggctggagca gagcgcgtaa
   135001 tcgcagtaga tccagtacag caccgtctag ataaagcggc taattacaat ggggttgaaa
   135061 cttatttctt agaagacacg gcacaagctg gaacagaatt gtatgaactg gctaaaggtg
   135121 gcgcagatgt cattatcgat tgtgtaggaa tggacggatt agagcctgtc aaagaaaaag
   135181 cgaaaaattt agtaagctta caaagcggca caatctctcc attacagatg gcatcgcaag
   135241 ctgtcaaaaa atttggaaca gtccaagtaa ctggtgttta tatgactcca gcttcttctt
   135301 acccgctgca agaatttttt atgcgggata tcgctgtcaa gcatggacag gcaccaatta
   135361 tccatctaat gccaaaaatt tacgcaatga ttgcagaagg actattcgat cctacacaaa
   135421 tcattactca ttcgctgcca ctatcagaag cagcacaagc atatgagatc tttgacaaaa
   135481 aagaagataa aaatataaaa gttgttttga aaccttgaat tcagtaaaaa atacaggtga
   135541 ttttctttat gcccaaaatc aaacgaaata cccatgagat cgaaatagga aaagaagaag
   135601 atatcgaaat acgaggacgt cgcttccgct tctatttcca aaatcgttac acattgatct
   135661 cactggctgt cgatttatta accgggattt tctatatttt tggaagcctt gcttccttaa
   135721 ctaaaatccc cgatcgatat ggcacctatt tttatttggc tggtgctatt ttcttgacga
   135781 ttcgtcctat tctcagaatc gtaaggaaca tttttattta cgacgaaaaa aaagcaatga
   135841 agagaacaag caaaaaaaaa gaggcatgag aatttttcaa atctcatgcc tcttttttca
   135901 ccctatcgta tcactttgat acagacgagc ggtcgtccca ctttcttcct tgaccattgt
   135961 caaaaatgtc cactcgtatc gttcataaaa cgtggtatga tctgtaatta agtaaagttt
   136021 ttcgtaccca agttgatgca tttcttttct gatttccgct aatatccgct tggctatccc
   136081 ctgcttacgc ttttccggtt caacaaacag ggcacaaaga tttggtgtca gatcagggcg
   136141 gttatggaag tcgttttcaa tgacgcccgc tcctccgacg atggtttctt tttcatctaa
   136201 gaccacatac cattgaggaa tactttcctt attagcgatt ccagtacgaa tactttcctc
   136261 atacacaaca gcaggaactc cccattttga tgaaaaccaa ttcgcgcttt gttttactaa
   136321 ttctgggtgt tctcttaatt tgatccagtt atatgtaacc aatgtttaaa cctccgacta
   136381 aaataaaacg tttacaaaaa ttatacaagt ctttatgaaa attagcaatg acaacaatag
   136441 tgatgtaaaa aaaaaaacgg aaaacaatca cttgttctcc gttttttgat taatcaagca
   136501 tctaaagctt cttttcttct ttcttggatc gtagctacct tgacgaacgg tagatagatc
   136561 aaaatagaaa gaaccaaatt gacagcggct agtacaccac ctgcaatact ttttgttgcc
   136621 aatacaccgc caataattgg tggagttacc catggaggca taacggtagc tgctggcact
   136681 aaacctgtag aagtcgcaaa gtaagcaaca ctaactaaga ccattggtgt caaaataaat
   136741 ggaatgaaca tgatcgggtt caacacgata ggtaacccaa acatcgttgg ttcattaata
   136801 ttgaagacac ctggcgcaat cgacaagttt gttacaacca tatatggttt gtttttacgt
   136861 cctactaaat aaatagctaa cagtaaacct aaagttgcac ctgttcctcc taagttgaca
   136921 aagctgtcaa agaaaggttt gttcacgata aacggtgcgc tatgtccagc tgcgattgca
   136981 ttaacgtttg cttcaatggc tggcgcattg attgtttgca tcaaaggatc gaccatattc
   137041 gctccgtgta aaccaaagaa ccataagaat ggtgtgataa atgcaagcag taaggcagat
   137101 ggataagagt ttgcaagtcc cataaatggt tcttggacca ctgtatagaa agaacctacg
   137161 atatcatcaa caccgaatgc agcaaaaatt gcagcaacta aagcaaacgc agagatagtg
   137221 atcattgctg gtagtaatga agcaaatgct tttgcaactg ccggtggcac accatcaggc
   137281 attttgatga tcaatttttc atttccgctt aaacgtacaa agatttctgt tgaaataatc
   137341 ccaacaataa tagcaataaa caaaccgttt gagctcatac ctaatgctcc gcctaaagcg
   137401 aagaaagagc ctaaagaaat cacacctgcg gaaacaccgt cttttcggta gccgttagct
   137461 aaatgataag caagcaagaa agcaatcaag acagaaagaa tggcaaatgt accgttccaa
   137521 gcagcagccc cgaatccttt ccatgattca ccaccaaaaa ttgagttcat caattcttgg
   137581 aacccgggaa tcggtaagtt attgatcatt actgctaatg ctcctaagat cattaacggc
   137641 atactcacca taaatgagtc ccgaatcgca actaaatgcc gttgtgcacc aatttttgat
   137701 gctacaggta taaaatgttt ctccataaat gcaacaaatt tatccataaa tcttccctct
   137761 tttctttact gtagattacg cgttcaatcg acgatacaga ttaatgatct cttttgctaa
   137821 atctgtaaaa gctatagatg tcatcagatg atcttgactg tggaccgtca aaagcgtgac
   137881 ttcaacgtga tttccttgtg cttcttgtgt cagcatctct gtttgagagt gatgtgcttg
   137941 gactaaagat tcctctgcat ctttgatttt ttcatctgct aaagtgaaat cacctttttt
   138001 cgcagcagca atcgcttcca ttgcatcgct ttttgcattt ccgccaaaca tgatcagacc
   138061 catgatggct tctagatttt tttgatcttc catttattgt atcctccgat aatttttatt
   138121 atttgtttat tagtttttct gcttggtcga gtactttttc accattcatc atgccatagt
   138181 cagccatatt gattacatct aaaggaatgc ctttaggtgc tagcttttca gcaaattgat
   138241 tttgcataaa gcgtacttgc ggtcctaaaa gtaaaacgtc tacattttta gattctagtt
   138301 gattatccgc atctgaagca gatacagcaa agatgtctgc ttccattcct tttgcttctg
   138361 ctgccttttg catttttgtg actaggagac ttgtactcat tccagctgaa catacaagca
   138421 tgattgtttt cttttccatt ttaaacacgt tcctttctat acttacactt gtatataagc
   138481 aagtagcgtg ccaactttaa aagcgcttta aaaagttgat atcatcacat ttaaggtcta
   138541 taccaagttg catacacact aaaaaagaaa gcgcacacta aacacacaaa ttaattttta
   138601 ttaccttttt tcttttaacg tgcacaaaaa aagttgtgac acaaaacttg tcacaacttt
   138661 tttcaaataa acggtattca aaagcttgca tctgcggtaa taagtccaac caagttaaaa
   138721 acataagatg ttgtttttac tttgctattc ttattacttg atgcaggaca gctttttcac
   138781 aacctctcaa taaacggtgt tcaatcagct gaccctgggt attagctcaa aacgctggat
   138841 tcacaacctc ttttatttca aataaccagt aaatacaatg aacatctaca aaaaacttct
   138901 atttatttat agatttcgtc aggcaaatcc aattctctct tatcaatcaa agtctgatac
   138961 cacgaagcag attttttcaa tgttctgctt cgttcatcgt tcaattcgat tcgaactaat
   139021 ccatatcgat tcttaaatgc attcattggt gacacacagt cagtgaatgc ccataacata
   139081 tagccttcgc aatttgcgcc ttcttctgtt gctttgatca agtaatgcaa atgctgtccg
   139141 ataaattcga tacggtaatc atcttgcacg attcctttct catccatata ctgttcttca
   139201 tattctcttc ccataccgtt ttctgttatc agccaaggga tattatgata attttctttt
   139261 agatacatgg ctatatcata catgattttc ggatagattt cccatcccct ggaattattc
   139321 attttcttcc caggtaaatc aaagacttca taatatcgtt caggatggaa aggtgtctct
   139381 tcattccatt gataacgagg ggcgcgaacg cgtttaggat aatattgatt gattccaagg
   139441 aaatctactg tatgatgctg aatcgtttct aaatcctctg ttgcaggatc gaaataaatc
   139501 gattgttttt gacacaactc caggaactct tctgtgtagt atccttttac catcggatca
   139561 aaaaatactc gattataaaa caaatcatac atataagcag ctttttggtc ttctggagaa
   139621 ttggatcgag catagaccat ttcaggattt aaaatacagc caatccttcc accataattt
   139681 ccgctgcggt aaatctctac tgctttagcc gttgctaaaa ctttatgata attccacatc
   139741 atccactttt ttgtgttttg ttcatgcggc cagcgaacag catccaaata gcaacgcgtt
   139801 tggggaacga ttggttcgtt gaatgtaaac cagtttttta ccctatcacc gtaacgatca
   139861 aaggcaattt gagcatactt agcataaaaa tcaactactt ttcttgaaga ccagccatca
   139921 tatttttcca aaagaaaagc tgggacttca taatgttcca aacaaatcat cggttctacg
   139981 tctgcttcga tcaaagagtc gatcatttca cttatatgct gagcatattc ttcatctact
   140041 atacagttct cataatcagt aaaaaaacga gcccaattga tagaagtccg ataatgtgtc
   140101 agatggatgg atttcatcag atccacatcc tgctggtatt gttccataaa attagttgct
   140161 acggctgggc catacccatt atgccaaaca aaatgttcat ttttatacca gctatctata
   140221 aacgaatcct gtccttcttt ttttcctttc caaccttccg tttgccaagc agaagatgca
   140281 gcaccaagaa taaaattttt tggaatagta attttcatga tttcctcctg ttttttagat
   140341 cccatcattt tccacacgat ttgctacttt tacaaatggc agatagatca aaatagaaac
   140401 aataatacaa acaagttgcg taataactgc accgatattt cctgctgttg ccagataagc
   140461 attgacgatc ggcggcattg gccaagggac cattacaact gctttcgccg caaatcctaa
   140521 agcggttgca aaataaccaa tcgttccagt gataagaggc gttaagataa acggaatagc
   140581 taaaattgga ttcagcatga tcggcatacc aaaaataact ggttcattga tgttgaagat
   140641 cccaggagca agggataact tggtgatttg tttcatatct tcccgttttc caactaacaa
   140701 tactgcaatc agtaaaccga tcgtgactcc agacccgccc atactcatat acatatccca
   140761 aaatggcata gtaataatgt tcggtacttc ttttccagct tcaaaagctt ccgtatttac
   140821 agcaattgcc gctaaaagca acggttcccg tatcggcttc accatttgat ttccatggat
   140881 accaatcacc caaaagactt gcgcaataaa catcaatagc aaaatacccg gtaacccttg
   140941 tacgacacct tctaatgggc gttgtacgac attgtaaatg atatcataaa ggtacattcc
   141001 tgtaagttct ttgatgataa atcccgcagc cgcgataccc gatacagtca gaatcgtcgg
   141061 gaataaagca gaaaaactag cagaaacatt cgttggcacg gtatcaggca ttctcagctg
   141121 tagtttcttc tgtttcctca accagcaata aagttcaata gaaaggatgg agaccaacat
   141181 tcccaaaaat aaccctttcg tatctgtata ttgtttagcc aatacgtttt ctactataac
   141241 agtctgattt tcattcacta attcataaac agttggatta acagataaat aagacatcac
   141301 tgcgatgata ccagggaata caccatccac tttattcagc ttggctaatt cgatccctat
   141361 cagaaaaaca gcatagatgg tcaagaaact tagtgtgaca tagctgatgg cagatgcgat
   141421 aggctttaat tcttctaaaa aacgcaagaa acttatctgt gctaagccat tttccgagtc
   141481 aaagaccatt gctgaaaaca atgtgccaaa agcacccgta ataattaccg gaatcaatgc
   141541 agcaaatgcg ttttttatca ccatgatata ccttaaatta ttgattctcg ttgccacgtt
   141601 ccctaaaaca ttggctaatt tatccataaa actcattcca gatcccctca ttcctgcttc
   141661 ttctccttga cgtcatctct ttttgtctat tagtcagcgc ttacatttcg atgaacagaa
   141721 actatttact cctctttttt accaaatata aaacaaaaat tcatctgatg tatttatcat
   141781 aaaatacatc agatgaactg tcaattagtt tctttttctc ctattatttt ctgtaattct
   141841 ctccgattta ttgccatatg gacagtcatt gcatcaaacg cagcaagtgc attccgcctt
   141901 tcgatggcct gaacgatctc tgtatgaata tcgatcgtct cgattttcag atgctggata
   141961 tcataatttt tattaaacaa agcaattgat tcattaatga ttgggataat atgcagcaag
   142021 gctacgtttc cgctagcttc tgcaatcatc gtatgaaaat cgatgtctaa ttgaacatgt
   142081 tcttgtgtgc cttctcgaaa agaagtttca atcttttgcg ttaatttttt catttggacg
   142141 atttgattct tcgttgcata ttccgctgcc aaacctgcca tcttaggctc caataaatat
   142201 ctcagttcaa ataagtctcg tatcatccga tcctgatctt caatcaaaga aaaaccgagt
   142261 gggtcattgg caatccctcg cttatcactg acaaatgtcc ccgctccttg ccgaacttca
   142321 aggatattcc tggaagctag agcccgcaca gcttctcgca ccgtgcttcg ccccacctct
   142381 aaataatcgg acaattcata ttcattcggc agtttatctc ctacttgcaa ctgcatttcc
   142441 tttaaatatc taaggatctt gtcagtagtt acttctacta gggttttatt ttcttccaaa
   142501 ccacatacct ccttttcatc tgatgtattt tttcgtaatt attataacac gtttgaatca
   142561 tctgatgatt ttttgtatac aaaattgatt aatccgttca tagacataca aacgaagaag
   142621 atatttacca taaaaaagac taggataaaa ttcactccta gccttcgatc atttttattt
   142681 tgaacgacaa aaaatatgat gttcaataac agacgatctt cattcgattc ttgatgtcat
   142741 cttttgtttt ttttaatata tggataaaaa gtttgattac atcgtttcta ttctagaatt
   142801 tggtcgattc tcaattcttc ttctggtgag aaatctaaat tatccatcat tcgaacactt
   142861 tcttgtaact gacttaaccg acttgctccg accagtacac ttgtaacaga tttttgtcgc
   142921 aaattccatg caagggccat ttgcgctaat gattgtcctc gtgatacggc gattgtttgc
   142981 agtgctttga ttttttccag agttgatcca acttgttctt ctgaaaggaa tggtatttcc
   143041 ttacgatgtg ctctagaatc ttcaggaata ccatgtagat aacgatttgt cagcaaccct
   143101 tgagcaagcg gactgaacgt gattgcccct agcccttctt cttctaatac tggagataga
   143161 tcgtcttcta gtagtgcacg agaaaacata ttgtagcgca tctgatgaat aatgaacggt
   143221 gcttcctttc tttttaaaat ttcagtcatt ttttttgtat cttctccatt atagttggat
   143281 ataccaatat ataatgcttt tccttgtctt acaagttgca tcaaagcttc tgctgtttct
   143341 tccaaaggtg tatcagggtc tgggcgatga tgatagaaga tgtcaacata atctagcccc
   143401 attctttgga gactttggtc acaacttgaa atgatggatt tacgagagcc ccattcccca
   143461 tatggccctt cccacatatg ataacctgct ttgctagaaa taatcagttc atcacggtag
   143521 gcttgaaaat cttctctaaa aatacgccca aagtttttct ctgcagctcc agcagggcct
   143581 ccataattat tcgcaaggtc gaaatgggta attcccatgt cgaatgcgcc tcttaaaatc
   143641 tctctttgat tatggatagg atcataatca ccaaaatttt gccataatcc gagagataaa
   143701 atcggtaatt tcaaacctga atgaccggat ttacgatatt ccattttttc ataacgtgca
   143761 ctatctgcaa tatacaattt ttctcctcct catcaaatct ttttaaaacg ctttcttttt
   143821 tctattttta gtttactaca ttttaaagaa aaggagttaa tcaaaatgat tttttcgtat
   143881 atttttttgc catttgtttt ttactcgttg atagatcaga aattgattac gtgcttgctg
   143941 gattttttta taacgtttcg gttgtttttt gtaaaattgc tgatgatagt tttctgctgg
   144001 ccaaaacgta ccggctggtt cgatttttgt gacgattggt tggtggaaag ttcctgaact
   144061 gtctagtttc tgttttgatt gttccgctaa ttctttttgt cgttcgactt gataaaaaat
   144121 cactggacga tattgcttcc ctctatcttg aaattgtcca aatgtatctg tgggatcagt
   144181 aatttgccaa tagattgtca gcaactcgct ataacttatt tctcttgtgt cataaatgat
   144241 ctccacagct tccacatgac ctgtgtaacc accacttact tgatcataat ttggtttttc
   144301 aacatgtccg cccgtgtatc ctgacaaaac agataggatc ccttttttcg attcaaacgg
   144361 ctcgaccata caccagaaac atccccctgc aaatactgca cgttcttgat agtcagcatc
   144421 ttgcatggca tgcttggaaa agtcatattt ttgctcctta tcaaatcctt ccgtaatcaa
   144481 taaataaaag tccattacat caggcgtcag attcattcgg atagctaatg gacgtagtgt
   144541 tgcttctagt tttgataact gttctttaac ggatatattt tctcttatgt cattctttgt
   144601 ctgaatcaat acttttcgtt cccaatctcg tgtattcgga ttcaaaatca gattataaag
   144661 tgtttgaatg aatatttgtt cctcatccat agattctcct cctttccgaa gaagacaagc
   144721 tagtgacttt agctgtaatt tatcataaag tgaggaaata agcgattact tcatccccct
   144781 acagattttt cgtataaaaa agagagaatc cttatcgaca gattctcctt tttaatcaat
   144841 ttattttata aatgaaaagt taatttacct tttatggaaa actattttaa aaaataaaca
   144901 taaatatgca gttaatgtaa caagataaga aataatagtc gttactgtat aacttgtgct
   144961 tactttaata tcagcaactc ctttgactcc gcttatttgt accaatccat tagcattcac
   145021 ctgctccttt acaggttgat gatttatttc tacattcgtt cctaaataag catatacagg
   145081 aagtgtgatt acttggctct tctcattagt tactctatat atgatagaac taccagttgt
   145141 tttataagga aggtttaccc attgactgcc aatctttgct tcgtgtttta ttatatgctc
   145201 cttattctcc aatgaagcga taggagaata atcatataaa aaataatcca ttcctttagt
   145261 ggtatacgcc tctactgttt ctttgtttac aacaatatgt cccggctgag aaattatttt
   145321 ttgattgaaa ttttttttac tagaaacagt taaagtgatt aataacaaaa cagtcataaa
   145381 taaataaaat agttctcttg tcttattgta tcttctattt ttgaaaaata tcatcgttaa
   145441 tatcagagaa gagaatatta tttgcaagct taagatccgc caaggaaatt gtaacaattt
   145501 ggctggcgta ttttgcagaa gataccatgg caccaaacta gttgtagcta gccaagtcaa
   145561 acacgtacca taataaatct ttttttctat gtttgttaat tttttaaata aaatcagggg
   145621 aatacataaa gataacagac caactagtcc cattgagtaa gtagttaagt cgttgttcat
   145681 agcttcagtc agactatcga acacattaga tgcatgagca tataaatttg tcacgctagg
   145741 tttgttgatt ttttggtaga gcatttgttc aaacatcgga taccaaaaag cactcgtcag
   145801 aaccaatgtc attcctgccg cactaaataa ttttttaaat atagcaataa aatctgcatt
   145861 cttgaaacct ttagaaacca aacgaataaa aataaaaatg ataattaatg ctgtactcat
   145921 taatgttgat agtacatggc tgtacactag caaactcata ctcaatgcta gctttacaga
   145981 cggatacttt tcttcttttc cagagatgat ttgataagca taaaataata cagggataac
   146041 aagtgttatt gcgatagctt cggcaattgc ggaacgatag tagatgtcta ctgtccgata
   146101 agcagagaaa atatagagac aactaaataa aaatgccgct tttttttgtt ttaaaaaccg
   146161 ctcgccataa tgataagcta cgagtgttgt acacaagttc aataaccaaa cgtaaataat
   146221 atagcttaca atcaaatttt ttgtcatcca atataacatt acagctggaa aaaaagtcag
   146281 aaagggataa aaataattta caccatatcc agtataattg aatgtctcat aattaatcgg
   146341 actggtaaaa atagtcgata accccttgat tcgagataag tgaaaagaaa gatctccagt
   146401 ggtataaata ctctgtgtat ttttgaaaaa gatgaaagaa tatatactac taactataat
   146461 taatccactc aatattacta cttttttatt tattttcatt cttcttgttt atctcctttt
   146521 tagtcacata tcgagaatct ttcaacttat taggtgcaaa tcgtttaaaa cttgaataca
   146581 ccactaaaca ataagtcaga atactgatcc ataaagccat gagagcagtt ggagtatatt
   146641 ttgttgtaat ttctatctta ttttctccag catttacatc cacattcact aagccatgtt
   146701 ttttataaga gtcatttacg actttaccgt ttacttttac ctgtgtacct aaataagcat
   146761 atactggtac tgtcactttt tgctttttgt tgcttgccac tgtgtaagta atgttattat
   146821 ctgcagcttt atacttccct ttttcccacg attctcctac ttttacttca tgtttttcta
   146881 agtgaggcct ttcttttatc gcttctgttg gggcgtaatc gtataatcct cccatatctg
   146941 atgttgtata acgtgggagt gtttcttcat tgataacaat atgaccgtgt tttgattgga
   147001 taacttttgc gtaattttct tttgctgcta tagacgtgac gagtagaaac accacagtag
   147061 cgccaatgat taaccatgaa tattttttat tcatagtctt tttcataaaa acaagcgtta
   147121 tgatcattga agaaaaaaag atttggatac ctaagatacg ccaaggaaac tggagtaagc
   147181 ttatcggtgt tttttgaaac aaaaaccatg gaaataaatt agttacgcat acccatgtag
   147241 aaactacgcc aacatagatg attttttctt ttttctccat ttctttcagt atgaataaag
   147301 gaaaaatcaa cgcaaccaat cctaataacc ccatagtgta tgttgtaata tcgttattta
   147361 acgcttgaat gatactatct gaaatagaaa gtgcttctag ctgcaaatct gttttgaagg
   147421 gactgtgtat ttcttgataa ttcatctgct gcaacatagg ataccaaaaa tagctagtta
   147481 atgcacaagt caaaaaaaca gcctttacta aattcacgac cacaaatttt atatcgtttt
   147541 ttatttgctt gcttaaaaac aaacgaacta taatcataca agcaatcaat gcactacaca
   147601 atagtgtagt taagacatga gtatacacaa gtagacttat actgacagat aaagcgatga
   147661 cttttttttc tctattgaat atcaatccat aagcataata taaaacagct gggataatcg
   147721 taagagcgat tgcttctgca attgcggatc gataatagat atcaactgtt cgataagttg
   147781 caaaagtata taaacaacta aataaaaaag cagcatctat ctttttaaaa aacttttctc
   147841 cataataaaa actaatcaga atcgtacaaa tatttaataa ccaaatatat agaacataac
   147901 ttaatattaa gttatttgaa attccataaa aaacgactgc aggaattatt gtaagaaaag
   147961 gataaaaata gttcagtcca tctccataat tgttaaatgt tgtatagttg attggccctt
   148021 caaaaataga agataaccct ttgattcgtg aaagatgaaa agataaatca cctgttgtat
   148081 aaatttcttg agcatttctc ataaaattta ctgaatataa agtactaagt aagataagaa
   148141 ctactaaaag aaatgctttt ttacttattt tcatagattt gttgttctcc ttttttaatt
   148201 ctattttcag tatactagaa ttgtcttttt ttaataagtt ttgcgttaaa tttttcatag
   148261 cactagtatg atatactaga aaagaatcaa tttttattac ttttaaatag gagaaaaaaa
   148321 tgaaaaaaaa caaaaaccaa ataatagata tcatttttat gttatttcta agttttgctt
   148381 acgtcatccc tctattcatg tctaagggaa tctatcatag tgtcaatcag gatacttact
   148441 ttcatttatc tcgaatcata ggactagata atgtatggag ttctccagta aacttcaata
   148501 acttcgatca tcatggaaca atgatgaata ttttttatcc atggcttact ctttatccag
   148561 cttttttatt ttataaaatg atgggaaatc ttgttttaag ttataatatc tactattttt
   148621 ttatcacttt tttaacaatg gtcgtttctt atttttctat gaaacaaata aaaaacaatc
   148681 gttatatttc gctgttattt tcgattattt atacattttc cgcctatcgt gccattgaca
   148741 tattccatcg tgcttcattg ggagaagctg tcgctttaac atttttgcct ttgatactaa
   148801 tggggtgtta cgaaatttat attagagatt accaaaaatg gtactggttg tctattggca
   148861 tgacgctagt agtgtatact catttacttt ctgtcgctat ggtttctgta tttattggag
   148921 gaacgttgtt tctttctttt tatttttggg accaaaaaat cgctagactc ttaagtcttt
   148981 taaaagctac cgtcctcacc ttttttttaa gtgcgggatt cttaattcct tttattcaac
   149041 aatctagagc acaagaattg aaagtcccac tgggaaaaga attaagtggt atggctccta
   149101 gtgatatgct gacacatatt ttaaataata actataacaa ctatactatt ggattattct
   149161 tatttctcgg attgattggt gcttttattt ttataaaaaa attaactgct gatgatttat
   149221 ttattttctt tcttggtgta tttgttcttt tttgttcaac caatttattt ccttggcaac
   149281 tttttaacca tacaccagtt aagagtctac aatttgtctg gcggttaaat ggtttttctt
   149341 ctttatttat agcctatacg atgagtatag tgatctatta tattttttct actaaaaata
   149401 attcatggaa aataatggtc tttacgtttc ttgcactaat attgcatctc tcaggagtaa
   149461 gcaattctat ccatgctaac aaagattcat taacacttat agcacctgaa gacgctgttg
   149521 ctatagctga aaattacaat catactgatt atgcaaacaa agagtcaatc tatcatcctg
   149581 atatgattaa taatgatcaa tatttactag gtaaccagga aataaatcca acaactcatt
   149641 ttatgagtaa caaacttaca attgaattag ataattcgaa taataaaaat actgtcttga
   149701 cgacacctat ttatcgttat aaagggcaag tggcttctat taatggcaaa ctagtacaaa
   149761 caaaattatc taagtttggc acaactgaat taactattcc tccaggtata aataaagtcg
   149821 ttattactta tcagtacaca aaactagcaa ttgcttcaag atatttatct atcgttactt
   149881 tgattttatt tttactttat cgatttactt tttctaaaca aaaacaaccg agaagagaag
   149941 tacaacattc tcactagatt tttatttaag aaagactttt tagctgtaca cataaacagg
   150001 ctaaaaagtc tttttttata tttctttaat ttgcttcata cattatttaa catcgatatt
   150061 tatactgtga aacatgcgtt tcatccatta tcaaagtatt tttatcgata ataaacttca
   150121 tccaaaaaca gaccttgtgc agggacggtt tctcctgcat gttgccgcac tttgctttca
   150181 aatatttcat cgattgtttc tatcggcatg gcacctgtgc cgatttctag taatgtcccc
   150241 atgataatcc gcaccatttt atataagaat ccgtctccga caaaagtaaa atgaagcatt
   150301 tctccctttc gttcgataga taattcatca atcgtacgca ctgtggattt ttttgatttt
   150361 ttcaatgcag aaaaaccaat gaaatcatgt gtacccacta atttttcgca agctgcattc
   150421 agcttgtcca tatccaactc ttgagggaaa taaaagctgt ggtttcgctc aaatgctgaa
   150481 ggaatcacgt tgttccaaac ataatagctg tattttttgc ccaccgcatt atatcttgaa
   150541 tggaagcgct caggcatttc ttcgattctt ttgatgacaa tatcccgcgg aaggtaacga
   150601 ttcataaagt cgatcatttc tgccagtcca atttctgtgt ttgttttaaa atttgctact
   150661 tgtcctcgcg catgggtgcc agcatctgtt cttcctgatc caataatttc tatctcttca
   150721 tttgtcatct gatgcaatac tttttcgatt tttccttgaa tggttttatc gtcatctcca
   150781 agtctttgcc atcctgaata tctttttccg tcatattcga ttgtcaactt gatgtttctc
   150841 atattttagt ggctcctctg tcttccattt ggtcttctct ctaccttttt aagattgtag
   150901 cacaaaatat agctccttgc ttctctaaga cagaagctat acaagaaaac tgtggaaata
   150961 tatatttcag aaaaacaaaa aacagccacc ggatgaagta tccgctgact gttcaatttg
   151021 acggctcaag ttttactgtt tgagatgcaa gatactcaac ctggcaatgg gcacataatt
   151081 aaagaaaaaa ctgattcttt ggcaaaaacc tattttattc agtaatggcc aagaatttat
   151141 agaaggaatt cttgccagtc cataaatcgc cgcaaaaagc aaactgatca cagacagtcc
   151201 aaggtataag tggcttacgt ccatttgccc attttttcga tagatcaaga aaagaagaac
   151261 tggaaacaat aaaaaaccac tgtaacctaa acctgatccg atattatgga cccatgtcga
   151321 gacattccac gaagattcat gggtattgat actgaacaaa cctgtaaaga tacaatcacc
   151381 tataccatag aacgcgatgg ctgctgtaac tagggtagct aacacacgag atgtactaac
   151441 aaatgcagca tacaatgcag gcaatgacag gatgaaaaag atccctgaaa ccacagacca
   151501 aataagaaac gcttttctta caggactgga aacctctcca aatacactga tcacgctctt
   151561 catttgatct aattcaggat aatacaatcc taatacgtac ggagtcaaaa aatcactcag
   151621 cacgcctaat aataaaaaga aaaaaccaaa tttacgtaaa aaatccatga tacgcctcca
   151681 ataacaatta cctatgcttt taattgttat tataaacgac tttatcttga tttaacagaa
   151741 acctctattt acacgaacat ctatataact cattaaaaac tcgaatcatt ttaaaaatac
   151801 taaatatcta acgttcaacg ttcacttaat ccatctaaac aatctttcaa tttgattttt
   151861 tccccacttc ttttacgcgc gcctagctct cctattgcag taaacaacag atcacttgac
   151921 gaatttaagg cagtttccat tgaatcctgg ataacaccga taataaatcc aataccgacg
   151981 atttgcatcg cgacatcatt agagatacca aacaaactac atgctaatgg aatcaataac
   152041 agtgaaccgc ccgcaacacc tgaagctcca caagcagcta aagcagacaa aatacataaa
   152101 aggactttca aaacaaaagg tgcttcaatc cctaaagaat gtacagcagt caacgtcatc
   152161 aaggaaatgg taatcgcggc accgcccata ttgatcgtag cacctaacgg gatcgatatg
   152221 gaataagact cttttgtcaa tttcaatctt tcagccaaca tcatgtttat cgggatattt
   152281 gcggcagaac ttcgggtaaa gaaagcggga agtgcgcttt ctttcaaaca aaagaaaatc
   152341 aatggatagg gattttcttt aataaacaaa aacgtgagga atgggtacac aactaatgcg
   152401 acaaacagca tcgtaccgat caaaacaagt aaaagctgca gatactctgc caatccagcg
   152461 attccagtcg tagcaattga ttgataaacc aatcctaaaa tccctatcgg agcaaatgca
   152521 ataatcactt gtacgacttg tgacaacgct atagatatct gatccaccaa ctcttttgtc
   152581 gattctgaac gtgctcgaaa agctagtccc aacaatacag cccagaataa gacgccaagg
   152641 taattcgcat caataagaga ttgaacagga ttttgtgtga cattcgtcaa catagtagat
   152701 aaaacacttt ccaaactttg cggtgctttt tcagatactg tttcttgtaa agtaatagga
   152761 acagtaaaaa ttgaacttac aataactgct acaagtgcag aaagcaatgt tcccactaaa
   152821 tataagatca aaataggctt cacgaatgtc ttattcccaa ttttatgctt ggcaattgac
   152881 gccatcgtca agaaaaaaac cagaataggt gcgattgcct tcaagcaact gacaaacaac
   152941 gttcccaata ttgaaataaa tgtccatgtg gggaaaaaga taccaaacaa tattccgact
   153001 aaaataccaa caacaatacg ttgaactaac gacatatcca aaaacttctc taacaaatga
   153061 ccaccctttc acaattttaa caatcattgt atcattttat gcaaagaaac taaaggtata
   153121 attttctaaa aattctaaca atacaaacaa ccatttcttg ctaattttgt actttttctg
   153181 tctacacgtt gttttttgag atattctaag ttaggatgtt attaatgggc aacttatcag
   153241 gaaacgtttt cttatgtttt tgtccaattt ttttgtgagg tgtttattct tgaaaaaaat
   153301 aaagaaggag agttatggct tcctgacaat ctttgtcgct tgtataatga tggttttaca
   153361 gcgtatattg atttcagcag tcactattcc tacatatgca acaaaccctg gtcgattcta
   153421 catctataca tttttacaaa cttccgttgt tattggtgca aatttgatcc cactttacat
   153481 gggttaccaa tatcaaaaaa tcgcccgttt aaaagtttta cattatttaa gtcgttttgc
   153541 ctttatattc ctaatagcca cactagtttg caatatcttt ttttacgtac aagctggcag
   153601 cttgaatatt cgtgattatt ggcttatcct aacacctatc agtcagaatt actttactta
   153661 tgctgtttct tgtactttgc ttttttgtag tattccttat acagttggat ttttaaataa
   153721 attatcgaaa acaacaatta aatatctact attattttta acaattggat tagttggctg
   153781 ctctactcta tttaataaag acccgtttgc attacagaat ggacagagta ttttatggat
   153841 tttttattta tttttaatgg gttatggttt gaaagaatgg aactggaaag aaaaggtccg
   153901 ctataagctt cctcaagtat ttatcagctt gattgtttta ttcggtttga ttattttgat
   153961 gacacaaatt tcactcatca tcagaggaaa tacagataca gcgctgaggt tttctaaacc
   154021 atattctctc ttcgcaatgt attatacttt ctcttcgttt ttatttttag aaatgctatc
   154081 tgataaatta ggtataaaag gaagaggaaa ttttcttgct gtatttttga ttgtcactca
   154141 agttatatgt aatttccctt tagtttctta tcgcgtatct acatttatga aacgtgaatt
   154201 tccaaactca gggaaggcat ggctgataaa tatcgggcag ttttttattt gatatttatt
   154261 agcagtgtcc ctcctagtta tctttttatt ttatatacaa aaaatccgtt tcattcatcg
   154321 tttcatcgaa tacttcgctt ttgattcgat tgaacaatta actcagcgtt ggaaaaaaag
   154381 aattcattgg ctttttgttc ataaaagtta ttttttagtt gcagcatttt tttactgctt
   154441 taccttcgtg cagattttcg ttttagaacc aaaagaaggt tggaaagaaa ctatccaggt
   154501 tgccttaaaa atatttactc agcgacaagc acccatgatc ctgacaatca tcattattat
   154561 gggatttttc ttcttactat tgttgatttc caatcgtttt tggtacgcct tgactgctac
   154621 cttgatcatt aacttactgc tcactatttc gacagtaatc aaaatggaaa tgcgggaaga
   154681 gcctgtcttt ccttcagatt taaaaatgct gaccggattg tcagaactat tatcgatggt
   154741 tagcccagtc ttattaatcg tgggcggatt gatccttctc ctattattga ttactagtat
   154801 tattgtacaa cgtagactac aaaaacagta ttccttgaag attcattgga aacgtcgatt
   154861 tatcagtatc gctgtattat taggatgttt ttcgggtgtc tttttcatta atcacaaaaa
   154921 ttcgccttct ttcttgctgt ttaatttatt taaagtaaac aagactttct ttaaccagca
   154981 aggcgccgta aaagataacg gaccagtcat tcaattccta aacaatatcg acattaaaat
   155041 catggaagaa cctgctggct attcgaaaga aaaaattaat ggaataatga aaaagtacaa
   155101 cacaaaggca gatgaaatca accaaacaag aagtgactgg ttaaacaatc agacgatcgt
   155161 tctcaattta agtgaaagct tcagtgatcc tgcacgtgtc cctaatctga cagtacctac
   155221 agaccctatt ccttacatca aaagcattat aacagaagga acaggaggat tgatgttatc
   155281 cgtcggttat ggtggcggaa cagctaacat ggaatgggaa ggactgacag gcttgagtat
   155341 cagtaacctg tctgcttcgt tagttacacc ttatacccag ctagtggagc gtcagaaaat
   155401 aagtccaaat attaccgatc tattcgatga aaaaatagcc atccatcctt ttacagctgc
   155461 tctttatcgc agaaaacaag tgtttgataa atttggtttt gacaggttct attatttaga
   155521 cagtccggat aaattaactt atacggataa aatagaacaa agcccacgca tatcggatga
   155581 ttcagcatac aaagaaacct tgaaagcttt acaaagcaat acggaaacgt cacaattcat
   155641 ccaattatcc actatgcaaa accatatgcc ttatgatgat tactatagtg aattgaacta
   155701 cactgctgaa ggaactgctg tcattcctaa tagaagaaaa gaattacaaa cttatatgca
   155761 aggccttcac tataccgata cagcagtaaa aaaattcatc caagaaatcg acaaaatcga
   155821 taaaccgatt acctttgttt tttatggtga ccatttaccc gcaatctata gtgggaatga
   155881 ttcgaaaaaa tatggattag aacagcacca gacagattat ttcatttata acaacaagta
   155941 tagtcgtgaa caagcagcta cgttaaataa agaagttgtt gctcctaata actttggtgc
   156001 tatggctctt gagcatgcga atatcaaagt aactccttac tatgcgttgt tgacagaagt
   156061 atctaatcat ctacctgcat taacaattga tcctacagaa agcttgtcta atcgttttaa
   156121 tggaaaacag gtctttgtta cagaggatag tcaaattatc caagaaaaaa atttaactga
   156181 gaaacaaaaa gaaattctag aagattatcg tctgatacaa tatgatcttg ttgcaggtaa
   156241 ccagtacagt gcaacatggg cgacacaaaa aataaaagac taacaaataa actgagcaag
   156301 gggttgtgat aaaagacatg tcacaacccc ttgtttatct cattaatttg cttgtccata
   156361 aacgatttga ttaaagccat ttttcaattc attattcgct gtctcgttga agtaaagata
   156421 attatcggga ttcccctgat tttgttgtgt caccaatact tttggctgat tttgctgaaa
   156481 cccgaataga tagacgtgct ttttcagata tggttctgtc tctgcatcgg aatagacggc
   156541 tgtgatttga tagtctgctt gacctgtccc actttctgac cattctgcag aaacaggcac
   156601 tccaccaata gccatcttcc actccccgtt caaaatagct tgagggactt taagaccata
   156661 taagtccaca ctcatttgat tcgtataact tttgtattgt tgtcccattg tttggcccca
   156721 tttggtcata aatgcctcta attgtgcgga tttgtctgca ttccacagtg tttgttcaac
   156781 ttgttcgctg ctttctgaag aggaagatga agcctctaca gaagaacttg aagtagatgt
   156841 ttgagttgtt tcagtcgttt tggaagactc tgacgaagta gaggtatctg ctgtttctgt
   156901 acttgctgca gttgacttgc tctctgtgct agttgttgta gtagttgcaa ctgttgcttt
   156961 cgctgtttct tttgttgatt cttttttcga ttcattcccg ttggtacaac cagctaacaa
   157021 aaaagctaga ccaattgtta ctaaaatcct ttttttcaat taaaaaactc ctttcgatta
   157081 ctcccacttc tttattctat caaataagca atgtgatttt attgattttt cgtattttct
   157141 catcaatctt tcaaatcttc taaaaactga gaagcacccg ctgatgttgt ctgtaataca
   157201 tcagcgggtg cttcttttat tttaatcttc gtgattttcc aatttacctc acgctttata
   157261 tttgtatttg aagaaacata gacttttagt catcatcgtc ttcctcatcc aaatcttgtc
   157321 ggacagtttt tccttggagt ctttgtactt ggtagatgat ttctttattg aaacttaaaa
   157381 gttcaacaag caattgttgc aagccatatt tttcttcttt ttcagctaat ttgattgccc
   157441 gtgtgataaa gacattttgg aaatcaaaat cttttgcaaa gttttccaac attgttgagg
   157501 ctggtaaata tttgttttga ccatgttcgt aaatttttcc ataacgcatg acttcttcta
   157561 aagtagacgg aacgatttca ttttcaatca ctaactcatg agagatttta ttgaaccatc
   157621 tcgcttcttg atcagcatat tctgaaaaaa gggtacttaa ttcttgcgaa gatgtaccca
   157681 aaacatacaa ttgtgcttgg atcaatttca tttcttctaa tttaaaattt gctaatacat
   157741 gccccaccat ggcgccagca gttggtttat gatggtcgat atctgcttgt tttgtttctt
   157801 gaacaaattt ttcttccact atattcattg atttacgcct cttttacttt tgctgattgg
   157861 acttcataac ctaatttttc cacaactgca gctaattgtt cagggtggac ttcatctgga
   157921 tcaaagtttg ctttgatttt gctcgcatta aacaaaactt tcgtattgct taccccaggc
   157981 tgttgagaaa ccgcatgttc gattttttgc atacagcttg gacaagctag tgtatctaat
   158041 tgcataataa tttttttcat cgtagttctt ccttcctttt tcttgatagt cttattctag
   158101 attacagctt ccataaaaaa attgattctt atcaagtttt acgatttttc ttctttgctt
   158161 tcgtgtccaa aatacaataa acgcatcgcg ttaaagataa cgatcaagat actagcttcg
   158221 tgaacgaaca tcccactagc catgtaaata aacccggcaa ataagccgtt gagtaaaaat
   158281 aagacgacac caatggcaat cactacattt tcacgggtat ttaaaacagt tttcttagcc
   158341 aagcggtagg catgaaccaa cgattcaaaa ttagattgca tcaagacaac gtcactcgtt
   158401 tcgatagcaa catccgttcc tgatcccata gcaatcccga tatcagccgc agccaaagag
   158461 ggagaatcat tgattccatc tccaataaaa gcaacgcgca atccttgttc ttggtatttt
   158521 ttcacaaatt gaaccttttc gtctggtaat aattcagcat ggacttcatc aatgcctaac
   158581 atatcagcta cataatcagc agtcatctga ttatcacctg tcagcatcac taactgtttt
   158641 gctcctgctt gtttcaactt agctaattgt tcagcgactt ctggtcgaat cacatctgac
   158701 acacctatga tttgagtgat ttttttgtcg attgctacaa taatgaccga gctgcctgtt
   158761 tgttgtaatt gatgtaaatc ttgtacttgc tttggtgtga gagaaagttt gtgagcggca
   158821 atcaatttgg tgttgcccgc acaaactgta tgaccatcaa tctcagctgt gattccttgt
   158881 cccttgattg tctgattatc tttgactgct aattgttgaa aatcaatgtc tttctcctga
   158941 gcataatgga tcacggcgcg ccccagagga tgatcggata acgtttctaa tcgagccact
   159001 aaagctaagc tgtccccttc atcctctgta tagttcttga tagtggatac agcagtagtt
   159061 cctttggtca atgtaccagt tttatcaaag acaaaagtat cgattttgga aaaagtatcc
   159121 attgcttcac cgcctttgac taagatgccc cgtttcgctc cattaccgat tcctgctaca
   159181 ttcgaaacag gcgcaccaat gactaaagcg ccaggacaac caagaaccaa aacagtgatt
   159241 gctaaacgaa aatcacgact aatcagacca acaaccaacg caataagtaa aacggttgga
   159301 gtataatact gagaaaatcg atcaatgaat ttttccgcat gggatttggt atcttgtgct
   159361 tcttctacta attcgataat tttggcaaag gtcgtatcat ctccgacctt agttgcttca
   159421 atcgtcacgt agccatctgc taaaatcgtt ccagagaaga cttcatctcc actgattttg
   159481 acgacttgtc gtgattcacc agtcactgcg gcttcatccg catacccttt cccgtcaatg
   159541 actcgtccgt ccacaggaat agctccacca gtttttacta agacgatatc gccttcttcc
   159601 acgtcatcga tttccacttc ttcagtcgtt ccatcttctt ggacaacact agcagtagat
   159661 ggggccatct cggtcaaagc cttgattgac tcccgcgttt tggtcaatgt tttttgttcc
   159721 agatagctac caaacaaaaa caagaatgta acgatagctg actccttgta ttctccgatc
   159781 ataaaggcac caagcacagc aatagtaacg agtaaatcaa ttgagataac tttgactttc
   159841 actgcctgat acgcatggat cataataggt atgaccgcaa taatagaagc tatggctaga
   159901 gcaatgttat agaacaagac aaaaccagta aattttccga taaaaccaaa aataatcaac
   159961 acagctgata aggcggtgat atgatttcga tgctcttgga tccacttttg taatttcatc
   160021 tttctccctc ttttcctttc attcttacga gactaagtat agatacttat ccatcataaa
   160081 aaattgacca ctatcaagtt tagtcaaaaa ggcggttttc ttttggtaga atacactaca
   160141 aaaaatagtt agcaagaaga ctcactaaaa gtgatcttgc caactattgt cgtttctatc
   160201 gaaattttca ttgaatgatt ttcctgtaca gcatacatcg gagatctatt tctttatcta
   160261 agatttttat cttgctaatt ttttccttta gtctttcaaa tccacgtcgt tcatagaatt
   160321 gatacgtaca gccactatct gtaaaaagga atatttcttt tcctttctct cttttttcaa
   160381 attctgttag caacttggta ccgatgcctt ttgttttcaa atcaggattt gcagctaaaa
   160441 aaatgatctc tccatctggc gaatgcttct ttaaatatgc tgcaagcatt tgttcatttg
   160501 ctgtctcgta agctccttta ctttctttag caaatatatt ttgtaaaaaa tcgaatatct
   160561 taacatatat ttgctgtcca aaagaacgat gtttcttttt ttctcctttt atctccgcca
   160621 gcaaaacacc tgccaactcg tcctctgcat acaaagcaag aatttgagtt gctcgattca
   160681 actccatata caaaaaatat cttccgtaaa gattcagcag aagttcattt tccatatatc
   160741 tctcaaagtt cattccttca acagcaaaat agatagcttt tttgtaatct ttcttttcta
   160801 agggtttaaa agttagtttc atcatttcgc ctcctcaact tcaatgattg tgttatactt
   160861 acattataat agacacagtg tctactatct ataatcaatc atctataagt ttgtcgatta
   160921 agcgacagct ttgctttgat atgtcggata aggagatagg taaatgaaaa agacagatag
   160981 acgcgtcaaa aaaacagaaa aagctttggc tgaaacacta tccacattat tagtaaataa
   161041 aaagattcaa gcaatcacta tacgcgaact aacagaaacg gcagatgtac atcgttcgac
   161101 tttttataca cattataagg atatctatga tctatatgat caattagaaa gcaacttttt
   161161 tcgggatcta aatgaaattc tctcttatga tccagcccat agctatgagg aactatacac
   161221 tcgcttgatt gactatgttt acgcaaatac ttttttatat cgcttattta tcctcaacca
   161281 tggtgatatt tcgtttcaga agaaagttaa acaagttatt gaagaaaact atctgaacat
   161341 atggctatat gaagacaaga aaacgattat cacagaagaa atgagattct ttactacgta
   161401 ccacgtccaa ggttgtctat ccgttctcaa tcggtgggta gaaagcaatt acaagtactc
   161461 caaaaaagaa ttattagaac tcttacagaa actgaatgat cacatagaga aaatcatgcc
   161521 ttgaataaaa acatactaag cttagttaag aagaaccgta accttctcgt ataacaactg
   161581 aatcttattt agccaaattg tttactgaag attttttgag taaaatttct ataattccta
   161641 cttattgaaa agtagtttgt agtgctgctt ctccctctct aagtgatata tcttgttgta
   161701 ccaatcaaat tcacctcatc attaataaat tacttttagg ctaatttatt acaacttata
   161761 ggttgaagct gtaaccaatt tatagtcata ttatattttt atcatccagt ttattgaatt
   161821 cttctcgttt acctcaattc attttcaagg ggaatcagct catcatctaa cattttacgc
   161881 catgctcgct tcatttcttg tgggtattta aatggtttcg cttctggatg tttggcttta
   161941 tatccttcgt aatccttact gttgataatt gctttcatat taggaattgg atcttcacct
   162001 ggcatatatt gcagattaga agcatgtaat tttttctcag aaacaaacca ctcattggaa
   162061 aagacatgaa tcaaatcatc acttacttgt gtaaaatagc gacgtttaat gacaaagata
   162121 tcttcattgc tatctagtgt accttcattt ctttgtgctt aaattcaaag ataattttct
   162181 caataaagat ttttgattcc agtaagattc ctttactcct tttacaacat agcgttgtcc
   162241 tttgttttga aacatcttct tcccctcttt gccattaaaa atagcccaat tctctataaa
   162301 gaagattaag cttcattatt ataatataaa gtttaaattt ggtttattac ttactttttc
   162361 ttattctcta gtaagctaat attgaaaaaa atgtaatcag atcattattt ttatatgttg
   162421 taacttcctg ataagaaaac taattaaaag aaagaggtaa ctacataaac taattaaaaa
   162481 agctaacttc atagttggag gataaatgga agaaacttca atgattgttt ctccttgtgt
   162541 tccttcaatc gacactagtc ctattatcaa tctctctttt ttgtccattt acttttgcga
   162601 ctgttcccca ataatcaaat acaggaagcg ttattttttg tcctttagat tcatgtactg
   162661 tatagactat tttcgaatcc atcacttgat aaggcatagt atgccattcc ttgccaattc
   162721 ttacttcatg attctgcaaa tgttctttgt attgtaatgc ttccgtgggt gcatagtcat
   162781 aaagtcctcc gggaaggagt gtcgtgtaac gaataagatt ttcttgattc attaccaagt
   162841 gatctctctg agtgacaact ttatgttcat agtttgcttt tgttgcagca gatacagcca
   162901 acagtaataa aacaattgcc gttatattaa ctacttttct ttgcttagct gatgtgctct
   162961 tcatgaaaac gaacgataaa atcaacgagc caaacagtac ttgaaagcct aagattcgcc
   163021 aaggaaattg aatcatttga atgggtgtat tttgtaataa agaccatgga aaaaatttgg
   163081 tggataagag ccaagagact accgcactaa ggtaaatcac tttttctttg cgattaaatc
   163141 ctttaaaaaa gaagagtgga cagatcaaag aaataatacc aatgattcct accgtaaagg
   163201 ttgtcaggtc attattcatc gcaccaatca aactatccga gacatttaat gcttggtgtt
   163261 gtaaggtcgt tttatctggc tcattaatcg attgaaccat gatttgtcgg aacatcggat
   163321 accaaaagta tgctgttaaa agaatcgtca ttcctactgc ctttgacaac tccacaatca
   163381 accggatgct ttcttttttt cttgtttcac ttactacaaa gccgcctaag actagtaagc
   163441 caacggctat tacactcata aaagtggaaa gaacatgtgt ataaatgagt aagctcatac
   163501 tgactgctaa tggaattgca catggctttt tctcataaat cagtgcgtaa gcataataca
   163561 taactatcgg tataaccgta agtgcaatcg cttcagcaat agcagaacga tgataaatat
   163621 caatcgtacg gtacccataa aatgtatata aacagctaaa tataaaagcc gcatcgattc
   163681 tttttaaaaa ttttagtcca taatgaaaca tcagcatgat agtacaaaca ttcaatagcc
   163741 aaacatataa gatataacta acgattaaat tattacttat ccaataaaaa atgactgccg
   163801 gaaaaaaggt caagtaggga taaaaataat tcactccgct cccataatga ttaaacgtcg
   163861 taaaattgat tggaccagaa aaaattgaag ataacccttt gactctagca aaatggaagg
   163921 caatatcccc cgatgtagaa atagtatgaa catttctaaa ataattgatt gaataaagtc
   163981 cagcaataat tgctaggaga atggacaata ttattttttt ctttacttgc attacttaat
   164041 tatatactcc tttcatggat tgctttatct agtataactt aaattttaaa taaatcgtta
   164101 tttattttta ctctcactta aaaaagcact aaaattataa gagagggagg taatggtctg
   164161 aacaccaaga attgttccaa catcgaattg cttaactaat gagttcaaag cttaattatg
   164221 tggctcttat atttaccgag cgtggattgc gatctcttta gcgaaacaaa catctttcat
   164281 aaaaaaaaca gccatatccg ctttcttgga tatgactatt tactactact ttactatctt
   164341 tcattcttct tgtctcactt atgataaggc gctgcgtatc atgagtgaat gatattagga
   164401 aaacttcata aaataaatct tttatacatt tccacttcta gatggtcatt catgttagaa
   164461 gaatcaaact atttttttac tgtcgtaata atattaaaac actagaacaa atcaattaaa
   164521 ttccataaaa tataaataat agacctagca tcatctggta taaaatggat gctaggtccg
   164581 ttatatttac tcaaaattga ttctacgaat agatcttctt atcatttcta ttttagtagt
   164641 catgcgttgt tgttcttcta aaggtggtaa tggtataagt agtttagata aatttgtacc
   164701 atttacattt ggttgcccag ttccgtaaga aattttttct aataacttcc aatataatgg
   164761 cgagtctaag aagcagtcta cgtattctga taataatttc tcaactaacc taattcgaat
   164821 caagtaagaa gcaaaaacag attcctcact tatttcctta accaagaaac tttttcccat
   164881 agttcctcct gtccttgcta tcaaaatgtc attttcttcc aatcttaaat caactagttt
   164941 agaattacta atatcacagt aaggcacact ggaccaattt acacgtccct cttgtatatc
   165001 tgtgattctc aaaaacttaa cattgccttg ttttttcgcc gaatctgtat agccatattg
   165061 aatcttagta gaaatacttc ctaaataaac ccatagtcga acatcgctag atgatttttc
   165121 ttgtcgttct ttatgaacaa cagtgatcat cttgagttgt ccgactattt tttagttgca
   165181 gcaatctttt tgggacgctt atttgcatga agcaacgttg ctaagtctag taataaaaca
   165241 ttggctaaca caggattggc tttctcgcga tacgctgcgg cgccactttc aaacaatact
   165301 gctagacgat cttctacaac aacgatttgt tccaaatacg gtggaaattt gattcgtttt
   165361 aggatccggc taggagagtc ccgctcggac actaagagtt ccgaggtata aggaccataa
   165421 gaacgactgg ctaagagata atggccataa aaagccacac cttgaatctt atcagatgtt
   165481 tttctgctca ttgtcacggt tggttggatt ttgtctttgc ggcgaattgc tgttttcatt
   165541 tcagctatcg tcggcaatag atagaaagtc aactgtcctt caccttttag tgtgaagttg
   165601 ccagatacca gttgatcatt gttttgagtc aaatacgatg cttgtggaat ctctgagaga
   165661 tcagttgtca gccaataagc gattggctgt cccgtctctt cagatgtatc tgctaagata
   165721 tcgatcaaac gtaaagcggc tactcgtcca gtagcagatc ctgtgattgt caaccataaa
   165781 agttcccgtt ttgtatcata tactaagccc cctacatgtg gccgtttagg taaaatcagc
   165841 agtttgatcg gctgtcctgt ttctctatcc agtacatgaa taatagaatg atgctgatgg
   165901 tcatagcagt aggcagaaat aaacacaaaa tcttgagaaa aagccaaccc ttgcggcgtc
   165961 atccattgac aaacctcatt ttcgccggtt tccggcacca ccattcttgt ttctgttaat
   166021 ccaggaatgt tatattgttt tgcttcagcc ttttcccaag ctgcttgaac aataggatac
   166081 cgttttaact ttttttcaat atcctgatgt atccgttgct gcttccaatg cttaaacttt
   166141 aggacatagt aaacaagaag gccaatgacc agcaacacta aaatcattat tgctacccgc
   166201 tgtgtcattt ttttgcctcc ttgttctatt cgatcaatcg tccatatacc gctattaatt
   166261 accgtaacta catgtttctt ttactagcgt ttctttatag gatctttcat aatctcattt
   166321 atagtaacat taagaatccc ttattctact atagtcactt ccacatcaat attgcctctt
   166381 gttgctttag agtatgggca aaatgcgtgg gcttcttctg cgattttctc tgcttggtcc
   166441 ttctcaagtc ctggcaaaga aacttctaaa acgaccccga tttggtagcc cccatcttct
   166501 aattgaaata aagatacccg tgctttgact tcagaatcag actcaagatt attttcttcc
   166561 agcatgtgtt gaaccgcgcc gttgaagcaa gatgcgtatc cagccgcaaa aagttgttcc
   166621 ggatttgtcc cttcactatg gattcctggg cggtcgatct tcatgtgcat acttccgttt
   166681 ggtgcctcaa cttcgccatc acggcctcca cgattgatca ttgctgtttc gtacattttt
   166741 ttcattgaga tcatcctttc tttcgtcgtt ctatattaag tgtaacgagt attttttaat
   166801 ctgacaaagg taatgcttgc attcggcgac atcagttaga caacatcgat gatcagtgat
   166861 agaagcagtt tatctataca aaaaaagaag ggttctatga tatttggtgt ccttactcgc
   166921 cttgagtaac aacaccaaat atcatagaac cacttaatca gcttcacact gctttttcta
   166981 ctttcgtagt tgaaaaaaac ttaaacaaat catcggcaaa tagttaaaga aaaaacttaa
   167041 tcgttgaaag aagcccaatt gcttaaagaa ggcaaactga ctaatcgacg gaatccgtgc
   167101 taatccgtaa agacctgcaa ttagtaaact aataactgtt aaaacaagat aaaaatggct
   167161 aagcgtacct tgaccacttt gacgataaag cagaacaagt aacaatggga aaagtagaaa
   167221 gcctgcatag cctagcccag aacctatatt atggatccaa gtcgatagat tacaagagct
   167281 ttcattcgta ttgatactga ataagccagt aaaaatacaa tcacctatcc catataaacc
   167341 aactgttgca acaacaagta ttgcaagtgt ttttgatgtt ccaacaaata ggtgatacaa
   167401 cgcaggcaac gaaagaacaa aaaaaagtcc agatacaact gaccagacca agaaagcacg
   167461 tctgactgga ctatctacgt caccaaacac actaattacg gtagtcattt gattcagttt
   167521 gggataaaaa aggccaagga tatacggtgt cgataaatca ctcatcacac ctaataataa
   167581 aaaataaaaa ccatattttt gcaaaaaaat cattttcttc ccctactttt tagtgattat
   167641 tcgatacatc caacaatcaa attttttctc attttaacac atatttttat tctagttgtc
   167701 ttttgaacaa tcaataacat aaaggtgagt ttcttctaca ttttaaaaaa tcgaatggca
   167761 ctgaagagtt cagttgtcag catcaatctc ctactcaaaa aggcaaaaaa acgtaagtca
   167821 gatgttgttg acatctcact tacgcatcca ttgattgaaa atttcttagt aaaatctact
   167881 agcctaaaaa tttgaagact tcttctcgat catacagttt aaatactttc tcgcataaaa
   167941 attgagtaat tagtgccgca aaaacaggga tgactaacca acaaagaaca acaagaaagc
   168001 catttagccc tgcatctaaa gaagccaacg gaccaacaag tccgaccacg ccgaaaccag
   168061 cagaagcagg cgtacctgaa atagaaaata gagcgacagg aatagctgta atcactgcac
   168121 taactaaaca aggaataaga atcacaggtt ttctaaatag attaggcatc atcatcttca
   168181 tagcgcctaa agcaatcgca attgtcacac ctgacttatt aaccttccaa gagttcacta
   168241 ctaaaacaaa agtggttgca gctacgccca tcgcagccgc accagcagaa acaccatcta
   168301 gttggattgc cattccgatt gctactgttg aaataggaga gataatcaaa aaggcaaagg
   168361 aacaaccaat caagatagac attaacagcg gttgtaaggt agtgaagtta ttaatcaatg
   168421 atccaattgc agaagtaatt gctgttacaa aagggaataa ataaaaacca agtaaacctg
   168481 cacctacacc tacaacaatc ggtgttaaca caatcgacac tgacccaaaa ctatttccaa
   168541 tcagtaaaat cagcccgaca gcaatcgatg ctgtcagcat cgtattaata atatcgcctg
   168601 taccagcacc aacgtaaaca cccgtttcac ccaaatcagg tgtgaatttg actacaccag
   168661 aaccaacaaa agaagctccg gctacaacca tcattctcat cggatcaaat ccaaattgta
   168721 aggcaatcaa accaccaatg atcaaaggtg ttgctagttg aaaaataact gctaactgtg
   168781 cgatcgttac cgcaaaggga tacgcactaa aatattttaa aatggcacct aatactgcat
   168841 taggaatcaa tccaataatg attcctgttg ctgtaccagc gaggacttta ttcagaaaaa
   168901 ttttcggtga tattttttgt gaagtttcca ttcaatctct cctttatttt acgttcaaac
   168961 tatcttcttt tgcatggaca agctcagtca agaaatcaca ataaggcgtc gcaacaccat
   169021 atttcttccc ttttctagaa attgcgccat tgatataatc gatttctgtt aatcgatggt
   169081 tcttaatcaa gtcttgatac attgaaggat aatgtaaccc aattgtttcc gggtcaaaac
   169141 aagattcaca atgtgcaatg acttctggga catctagttc aatcttctct actgctgcta
   169201 ctttggcaaa ctcattgaca atcgtcgcca ccattttatg agcagtcgat gtttttccta
   169261 gctcagccat attaacgtct aagattgtgc atagtccgtt cattgttccg ttcacacatg
   169321 ctttgcgata aatagaatag tgaatattgt cagaaaaatg agcatttaat cctgattcag
   169381 acaatttgtc tgctaatttt tttgcagctg cttcttttcc atcacctaaa ttttgtaatt
   169441 ctactgaacc acttccaaat aatttgacct gaccaggacc ttcaagacct gccgtccaca
   169501 tggtatttcc aatatagata ttttccattg gtacaaattt ctcaataata tcttcatgcc
   169561 cgataccatt taatagacat aagacctctg tatctttttt gattaatgat tggatatcct
   169621 gcagcatttt ttccagctgc atcgctttgg taaatagaat aatcagatca acttgatctt
   169681 ctttttctac ttcggattga agaacgattg gtagttttgc ttctacctct ttaccattaa
   169741 aatttgcttg taatccatgt tctttgattt gttggacatg ttctgcccag ccatcgatta
   169801 ataatacttc gttcccactt tggtgaagca tcaacccaaa gcgacttccc attgcgccag
   169861 cgcctgcaat tgctattttc attgattctt cctccttttt ctttctatta tactcatttg
   169921 agaatctttt cacaacctcg ttttgttatt cttctcttat ttatcatgtt tatatccacc
   169981 attcaagtta ttttatcagc aaatcaataa aaactggaat agaatcccaa tttcatcgta
   170041 agagaaacta gactagcaat aagaaaatag aaacattcat agaaaaaatc tcactcctct
   170101 gattataaaa gagggggcat taacctcgtg tatactaaaa ccttccattc attcgatgga
   170161 tgattttctt tttgtgaaaa aatattcatt gtagtacgat gttgcgcaac acagacggat
   170221 ggtattgctt attatttatt aaaatcgaac aatacactta tttaaaaact tctttcccag
   170281 attttcttcc atttttacag ttataactag tattatcatg aaaaatagcc ttttattgtc
   170341 tatgggaata ataatttaaa attggtactt ttttattaat tagttcatcg cccaataatc
   170401 tctccagttt cttagcatct cctgactcaa ccggaattat ccaagcccag tctaaataat
   170461 tttttaaaat ctcatcatat tttgcaaata tttcattgcc atattccgtc cttaaaatta
   170521 tattttcagt gccagacaag tgatttatat tgagttgata aaaaagatct ccatctaaaa
   170581 aaatatcatt agaagaagat ttccccacag taatgaccat tccatcagct ctatgtgtta
   170641 catacaaata attgtatgac ctcatttcac ctaatgaatc aattatttct tgccctttag
   170701 ctctaatata ttgatctcta ttacctatct cactcttatt taacaaaagt tttttcatgt
   170761 caaatttaat cggctgtttt atttttgtaa attcgcttct actactaatc caatccgaga
   170821 ttaatttttc aacatgtccc agagtaaact ctgatttatt cgctaaatac tttttttgtt
   170881 gtattaaaga catctttccc cgaagctcaa aattgagcct acatcgattc tctatctctt
   170941 caaaaagcat cttatcttca tcattcttat attcatcatt cataaaacca atccagctcc
   171001 tcgattaaga taatatttta atctcataca aaaactagct ctcatcaaaa caaccattgt
   171061 tttgatttct catgaaattt ctttatactt ttctccgatt ataaattcct aaccccttga
   171121 gaaaattacg catatattta tctccgcaag gcttaaaatt tcgttggcct tcttttctca
   171181 aaaatgcccc gatttcgcct tttgatggat agatttctgc ctcagcaaaa acgtctaaaa
   171241 tatcatcagt ggtcatagac aaggcgattt ttaatttttt tagcaacaca ttgttgatat
   171301 atttgatttc cttgcttttt tgcggcgtgt ctactatcgg cttatctttt tgcggaccac
   171361 gcgccaagag aatcaggcca tttaggaatg cgtcaaaaac ttggttgttg atggtttttt
   171421 catacacatt attttctgca ttatctttgt tcttatcctg tactttggtc agtaccgcct
   171481 gagcttcttc tttagtcagt ggtacaccgc ctagctcaaa agctttaatc aaatccgtat
   171541 ctttcaaatc aatcgcataa cgcaatcgca ataacctatc attgttattc atttgttcct
   171601 ccgaagtatt tagtctttac tttcttacgt actttttgtc ttgctaagat ttttaggatc
   171661 aatgataaaa accgcatcac ttatgataag gcgctgggta tcatgagtga atgatattgc
   171721 tgaatacaaa tagtccgacc acattaagtt gatcggactt cataagcatt ttattgacta
   171781 atactttttt gcttaagaat tgattctaac atcttttgct aaagttaagc atcctatagt
   171841 tcctgcatta tctgctaact taggtgtgac aatgtattct tcgatatttg gcgtatcgac
   171901 atagccgtta attagttcta aaaaacgtct ttgtactttc tttttcagat gttcttgttg
   171961 catgacacca ccaccaaaaa ttataatatc tggtgaaagc atcagggtcg tattacaggc
   172021 acattgtgca atataatccg cctcgatttc ccagaacgga tcttctatag aaagatcttg
   172081 tcccttggtt cccgtacgca tctcgatagc gggtccagat gccaaccctt cgagacaatc
   172141 atgatgaaaa ggacaagtac cagagaaagt atctcctgaa tgtctcttaa ctaacgtatg
   172201 ccccatttct ggatggctaa acccttcaac aaaaatccca tcttgtatag ctcctccacc
   172261 aattccagtt ccaacagtgt aataaacaac actggataaa ccttttccat gacctgaaac
   172321 atactccccg taacaagcag cattcacatc ggttgtccag gcaattggaa tttcgaaatg
   172381 cttattcaac gttccaacaa aatcaaaatt tttccaggca gtttttggag tagaggtgat
   172441 atgtccatag gtctttgatt cacgatggat atcaatcggt ccaaatgacc ctactccaat
   172501 gctttctaat tgttctttat agtgattaaa gaaatcaatt accaaactca ttgtttcatt
   172561 cggtgtagtt gtaggaaaac tcactctttc taaaaccttt aaatcatcat tgccaatggc
   172621 acaaacaaat ttagtcccac ctgcttctat tgagccatac atttcttact ccaccgtcct
   172681 tttatctagg aattcgttct tccctgtcta atagtttttc gaaaaactca tgtggttcct
   172741 tttggtacca ataggcaaca gaagaaatat cgtcttgacg ctcataaagc ttgatgtcat
   172801 catttcctag ggtttggaaa gtaacacgta aattttcttt gaaactaatg gggtcgggta
   172861 aatgccagcg gtacaaccca tgcatcggta aagcatcatc gccaaaacca tgaactggat
   172921 ttggattagg ttttccagca ctaaaataat cccgggtgtg atctctttta gattgaaaag
   172981 gatagcccat aaatagcgta ttaaaatttt gagcatatgc cctttctcca aagttctttt
   173041 tatggaaagc ccaagcgccg ccaaaataat cttctgctcc tgtagatgtt attgtaggga
   173101 aatcttcatc tccatctata taaaacttaa attctccttc cccccaccag tatctttcca
   173161 atgcgcacaa agcaaagtag gttcctacat aatacccagt gcctttgatt ccatcaataa
   173221 gggtgtaatc ttttcctttt gtcgtgatgg gttcacggtt ccatttagca tgaaaataca
   173281 tcgcatcatc cggtacttca gtcaatgcat aattaaatgt ataaaaaatg tgcttaataa
   173341 actcaggatg ttcattcgtt aaagtaatct tagctttttt tcgaaatggc atctcaaaat
   173401 agcaattcat ccctgatgtt ggattcacta cgattggtaa ggaattcact tcgcatcttg
   173461 tcccaaaccc attacaaaaa aaatctccta atggactttc tacagaagga gtggattcat
   173521 catcccaata aatacgcaaa attaaatcac gtaagacgaa actcccagcg attgttttgt
   173581 ctgtcactgt catccaaaaa tgacgtaaaa ttccagggcc ttccatttct gctaaagtaa
   173641 ttgtctctcc agcatttatc ggaacagaag gtgacccttt tctcccaatt cctagatgac
   173701 tcgctgtcat tgccgctttc cctttttcac catttttatt ttctggtgaa atagttctac
   173761 tttcctctcc cttgtggagc catatattat ttaaacaatt cattttattc gtcctctatc
   173821 ctttcttcac ttttttgatt attcatatag atgatttgtt gttgtgcctc cgcaataatt
   173881 gctccaatat ctaaattatc atttcctaac tgcaaagcga ttgacattgc caagggaaga
   173941 ttcattcctg caattacgtg cactctgtca gaaatatagg taaaaaactt ttgatttaca
   174001 cttcctccag ccatgtcagt taaaacaatt atttcgtctg tagtatccac taactcttta
   174061 atttgttcat ctatatcatc aatttcttcg acatatgcac ataagctact aatttgatta
   174121 tgcatacctg ttaaaaactc tagtgtctct ttaaaaccct cagctagtat tgaatggcta
   174181 gcaatgatca atcttctcat aattcaaatc cttttccttt acgctaatat tccaaatgca
   174241 gccataacca tcgcaaaaac aataacgagt agaatcaaaa tggtcatact gatttttttc
   174301 ttactgagta gataatatgc tgcagcagta ataattactg gaattaatgt tggtaaaatt
   174361 ttgtccaaca ttggttggat tttcatacta acttcaccaa aagtgaattt aagtggcgtt
   174421 gaaacactga taactgaagc aattaatgat ccaactactg ttagccccaa tactgaagca
   174481 gcttcagtta aataattaat cttttcaccg tattcagtaa caatcttcat tccctgctta
   174541 taaccaaatt caaagcctct cattcggaaa aagaagatta ccatgattaa aatgaaccat
   174601 aaaaagattc ctacaggatt attttctaaa gccatataag ccgcaattga tcccatgatg
   174661 gttgggatca tcgtcatcaa aagagagtcc ccaataccag caattggacc catcgtacca
   174721 actttaaagt cattaatcgc atcttttgct tctaatcctt gcttttcctc cattgcaaca
   174781 caagcaccta aaataatggc tgccagccaa ggctgtgtat tgtaatactg aaagtgatta
   174841 tttaaagctt cgatgtattc atcatcatct ttgtataatt ttcttaaaat aggtgacaat
   174901 gcgtaagtca cagatgcacc ttgctgtgtt tgataattaa aggtacaaac tgacatcaac
   174961 caacgatagt tcgctttcct aagatccttt ttcgttactt gatatttttc gctcatttta
   175021 ttcatcctcc ccaataaacc caacatcaga agctcccgca gaagcagtga tttgtgcatc
   175081 tttttcagct ctatgataca cattaaatgc taaagcgaat cctatgatcg ccgctccaat
   175141 aataggtacc ttcaaaaaag cggctaagaa aaatccaaca agtaaataac taatgtatcg
   175201 acctgtaggc ataaagcgta acaacatcgc aataccaaca gcaggcaaca tttttccggc
   175261 taatgttagc ccatccataa accaattagg taaaacatcc aaaacgtaat taacagcaga
   175321 ctgtcctaaa gtaacggcaa taaatactgg aattgctgca gataaaccaa agaaaattgg
   175381 ccccaaccac aaaacactat tcattttctt aaattctcgt ttattcgcat attcctgtga
   175441 tttgcgaaca acaaaaccat ttaagatttt gacaattaca tctagttgca cccctaacat
   175501 acctacaggt agtcctattg ctaatccggc ttctactcca agctggctac caaatgccgt
   175561 tgcaataatt gcgcacgttc catagtccgg tacagatgag ccaccaaaac cggcaactcc
   175621 taatgacatc aattgaactg taccgccaac tatcaaacct gtttttatat cacccatcaa
   175681 aattcctgtg aataatcccc aaaaaactgc gtaatttaca aagatcatcg gaatcccata
   175741 ataatcaaca tgtttaataa atgctaatat agtcagcata ataatttgaa aaatagtaaa
   175801 atgcattatc cttactcctt tatcctaatt ttgattgaat ttcctgtgcc ttatccgccg
   175861 gaacaaattg tgtaacaatt tctacaccag aatccaacaa ttttttatac gtgctgatat
   175921 tttcatctga tgcataggct ctagaagata gctgtaatgc attatcttta acaacagtac
   175981 cgccaacaat tagtcggtca attctaatac cgttatctac taaatgaagg attgtttccg
   176041 gctctttcac gatgacaaat actttttcct gatcgtattt ccccttttta aaattcgcaa
   176101 tcgctgtttc ttcagtgata attgatacag ccattccagc aggtctagct aaacgcatac
   176161 ttgatttttg gatgtcgtta tttgcgacag aatcatcgat caccatcact ctgtttgctt
   176221 tagacattgg tgcccattgc gtgacaataa tcccatgcaa taaacggtta tcaactctcg
   176281 acataacaat actcataaat acttcctcct ttatctttca ttttcttaca agcaattacc
   176341 atgccaaaac aaaaagaact tgataaatca agctcttttt acataatcat aaacgtattt
   176401 aacctcatga atcgggatgt cgacactata ataatgttca acgacactga aagattgctt
   176461 aaaatgtgtc tgaaattttt gaaattcctc actcgtttca ttgaaataaa tttcatcttc
   176521 caaagtattt tgttttacta atcgttcaat taagcaacta atgtgaacat ataatccgaa
   176581 acacgtatta ttatcaaatg aaattcctag atcattttgt aagatgtata tagcatctga
   176641 aacccgttct aataatttag tcggatttaa tatggtcaat tgctccatta aattcgttag
   176701 actaaaattc ttcagaatat tattgttcaa aactttcagt tcattatcat taaagtgtct
   176761 attcaattga ttggagaatt tacttatatt cattcctaaa atcatatctt caattgaaaa
   176821 gaattctaca tcttctagat gtggatttag tgttccaaca ataaaaagaa tattgttctc
   176881 tttacgaagc tccgataaat agttttcatt cactaaagag taatagtcac acgtttctat
   176941 gacaagatca gtatcccttg gaaagctatc ttcaagtatc ttttttaatt taaacgaagt
   177001 tcctaatccg cttgcacaag aacagacaat catatccttc ttaatatttg atgattcata
   177061 aatataatcc acattttgat tttcttttac ttgttcaaga atctgtttaa gcggtaaatc
   177121 taataatatc tgatttgcaa catcaatagc cattcttgtt gaaacattgt ttagtagtcc
   177181 gaattttttt tgagcgtcta gttccaaatt tagataaatt ttctccagtg aacccatatc
   177241 aacaagtaaa ataatttctt catatccggt acactcttgt aagtatctat taattttgtc
   177301 tgcaatctca ctacttgaag tcattagtgg catatcaatg gcgtcgtata tatatctctc
   177361 aacgatccta ttagcagtac ttgctataca ggttgccgtt agatctccat gagccaaaat
   177421 cagtgctaaa cgtttttgct gaaattcttt actacacatt aacttaaata aaataaccaa
   177481 aacatttttt tgaatttttg ttaaataaat atcccctatt tgaataaaac gattgactaa
   177541 ttgaacttct cgactacacc aactattaat atcattgatg aaggaatata atttatcgtc
   177601 cgatgttaat tggatttgat aaatccaaga attcaaaaag atagacgtta tttgaagata
   177661 ataatagtca aattctattt gaaaatctct ctctatttcc ttaacatact gtctaaatcg
   177721 aatctcttga taggtgggat atgttaaagc tgaattattt tggatatcaa cattcttttg
   177781 tctaaaatat ctatcatatt tctcaaaaag ctgctgctta tcagtgtctt tcttccacgt
   177841 ttgataaaga tactctataa gttcgttgat attaaaaact tcgtctactt tttccaaatc
   177901 atccatttga atatatttta attcattctg tcttttcata accttcatgc cgttgaaatt
   177961 cacccattgt ggtaaatgat acatctgaat ataaagttct tccaaatcat ctaagaaata
   178021 aaaggcattg gcacaggtca attgaatgat atttttcaaa ttgccaacat tttcactaaa
   178081 gcgatagttt aataaaaaat ctaataattg ttgagatatc agtattttct ttgctagtct
   178141 ttcttcttct gatttaaaaa atatcttgat taactgtttt ctttcataaa gagaacgact
   178201 gtctaatgtt ggaagtgtta gctttacagg tattcttctc aacaaagttg ataatagttt
   178261 atcctcaggt ggttctgatg ttgcaaatat aatccagcaa cttgatgtgt accagttttc
   178321 attatcaccc atccgatgaa atttcctcgt atccataaac tgaaaaagtt tctcctgaca
   178381 ttcattgctc aatgcatgaa tttcatctaa aaataagact ccattattag ctaattctgc
   178441 taaacccttt cttccatctt cggctcctgt atatgcacct tttacatgcc caaaaaaatt
   178501 ggacaagaaa agttctggat tattggcata ttcactacaa ttaactgaaa tgaaagatgc
   178561 cttctcttct atataattat tggccaacaa atccttatac acataatttg ctaaatagct
   178621 ttttccagtt ccagtctcgc catgaagtaa aatgggtaag ccatttggcg gatagtagat
   178681 tgctgcttta atttgagcaa ttacatttcg cagacttcct tgacttccga taaccctttc
   178741 tattccaacc ctaaaagagc tatcatacca tttttccgct tcctctgaag agctaaaaat
   178801 ttgttgattt ggaactttct ccggtaaaga aaagaagatt actggacgag tattgatttt
   178861 tccaaattct ccatctttta cgaattcatt caaatattga gagacaatat tacgactaac
   178921 atttaatttt tcagatattt tctgtgctgt attgttcccc catttatttt taatctcaga
   178981 gttctctatg acataattga caatgcgttc ttttaattct tccatcacag cacctcctcg
   179041 acactattcg aattttgccg acagtatttt tccataaata tgacttgcta aaaaggcttt
   179101 gtttagatca ttcaattgga acgttgcttg ttttgcttga tccgctaaga cgttgaaaag
   179161 atactctggc gaaatcgcat accccaaaat atcaacgatt gttgcaatca gatcttcttt
   179221 tgattcttca tctgaaagta attcacgata caacatcgtt tgtttgctta ctgtgtcata
   179281 ttcctctaag gattcatctg ctaaaagaac cacttgttcc aacaaacggt cggataaata
   179341 tttatagaaa atcagcccca ataaataatt tttatattct gacgcatcca ttttactacg
   179401 gagattatct gccgcactaa ataacttctg atttaattct gcacccaaaa agatacactc
   179461 ctatctctta tttaaagttg ctatataccc tcttatttta gcatactcag ttttagaaaa
   179521 agcaaaaaaa taaagaaact tattcttgat aacaagctac gtgacgtata tccctaacac
   179581 aaaaatagcc atatcctttg gtttggatac ggctatttga tattactcat tcaattatca
   179641 aatcacagta ggtgtgaatc tcatcgggat cggcaatatc tacttctgat taggatcaaa
   179701 ggtgattttc tcaataaagg tgtgatacaa agctttaact tgcttcttct cactctggat
   179761 aagaaagcaa tccaaactag tcaatatctg ttggacatct ttgacttgaa tcggttcccc
   179821 ttgttgttga aagatcccta agatttcatt ctctcgaatt tgtaactgtc ttcgtttttc
   179881 agtcaattca tttaaacgat cttttaacat cggaaacaat tcgggactat cttccaaagc
   179941 agcttcctgt ttttgctcta atagttagat tttgcgtacc attttcttct tttattttcc
   180001 ttcgattgct gattttaata tagtctaaag gaaatttata tttattgtat tctttataac
   180061 ttcaagagaa cttcgtttga gtattactgt atttttttaa ttccataaga aaattagtta
   180121 ttacctgcca cctaatcagc aaatctgaac ctgagtcaat ataattaata ttattttcca
   180181 tctccaacat tgtaagtact gttggtgtta atatagggac tttgtacaat tgtatttttt
   180241 tgattttatc atctgcacaa aggagatttt ttaaatctaa tccttctttt atttcaaaag
   180301 ttgaattttt taaatctata agagtaaaac caattccttc atactgaatt tctgaaaatc
   180361 gaatttttct cccagttgat agatcaaggg gcagaaaacc ttttattaca ttgtgagtgt
   180421 caccatcatt tacaatgtca ttaggaattt tgattttaga attctgtgga gcacctattg
   180481 gaagataata attcattata attttgtagt cttcatgagg aaataaagta cttttaggat
   180541 tgctaagata ttctcgtacc gaatctaaga atggcttatc cattgaatct tcatccccat
   180601 ttaaactaaa aaacattcct acaattgctt ttgcaactct aagtgaattc attttcttta
   180661 ttgaatgtgt taaatcagac ttaccattct ttaaacttgt attgttataa aggaaatttt
   180721 tattgtcttc tgcaaatcta atatattcct caacatactc actaccaaaa aaactattac
   180781 atttactaca taacg
//
LOCUS       DARBFO010000002        93571 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000002 DARBFO010000000
VERSION     DARBFO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 93571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.002"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..562
                     /locus_tag="SCK51_000163"
     CDS             <1..562
                     /locus_tag="SCK51_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEG1250820.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            762..1076
                     /locus_tag="SCK51_000164"
     CDS             762..1076
                     /locus_tag="SCK51_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1250821.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1097..1474
                     /locus_tag="SCK51_000165"
     CDS             1097..1474
                     /locus_tag="SCK51_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1250822.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1484..2257
                     /locus_tag="SCK51_000166"
     CDS             1484..2257
                     /locus_tag="SCK51_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250823.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2279..3682
                     /locus_tag="SCK51_000167"
     CDS             2279..3682
                     /locus_tag="SCK51_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG1250824.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3864..5048
                     /locus_tag="SCK51_000168"
     CDS             3864..5048
                     /locus_tag="SCK51_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG1250825.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5045..5335
                     /locus_tag="SCK51_000169"
     CDS             5045..5335
                     /locus_tag="SCK51_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250826.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5332..5553
                     /locus_tag="SCK51_000170"
     CDS             5332..5553
                     /locus_tag="SCK51_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250827.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5595..6374
                     /locus_tag="SCK51_000171"
     CDS             5595..6374
                     /locus_tag="SCK51_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HEG1250828.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6441..6935
                     /locus_tag="SCK51_000172"
     CDS             6441..6935
                     /locus_tag="SCK51_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG1250829.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6991..7629
                     /locus_tag="SCK51_000173"
     CDS             6991..7629
                     /locus_tag="SCK51_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250830.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7701..8096
                     /locus_tag="SCK51_000174"
     CDS             7701..8096
                     /locus_tag="SCK51_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1250831.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8080..10533
                     /locus_tag="SCK51_000175"
     CDS             8080..10533
                     /locus_tag="SCK51_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1250832.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10530..12557
                     /locus_tag="SCK51_000176"
     CDS             10530..12557
                     /locus_tag="SCK51_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250833.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12554..13576
                     /locus_tag="SCK51_000177"
     CDS             12554..13576
                     /locus_tag="SCK51_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEG1250834.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13593..14522
                     /locus_tag="SCK51_000178"
     CDS             13593..14522
                     /locus_tag="SCK51_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1250835.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14767..14883
                     /locus_tag="SCK51_000179"
     CDS             14767..14883
                     /locus_tag="SCK51_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEG1250836.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14899..15702
                     /locus_tag="SCK51_000180"
     CDS             14899..15702
                     /locus_tag="SCK51_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG1250837.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            15937..16173
                     /locus_tag="SCK51_000181"
     CDS             15937..16173
                     /locus_tag="SCK51_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250838.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            16180..18132
                     /locus_tag="SCK51_000182"
     CDS             16180..18132
                     /locus_tag="SCK51_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEG1250839.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="SCK51_000183"
     CDS             complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="SCK51_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEG1250840.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            19063..20103
                     /locus_tag="SCK51_000184"
     CDS             19063..20103
                     /locus_tag="SCK51_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG1250841.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            20201..20389
                     /locus_tag="SCK51_000185"
     CDS             20201..20389
                     /locus_tag="SCK51_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEG1250842.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(20447..20800)
                     /locus_tag="SCK51_000186"
     CDS             complement(20447..20800)
                     /locus_tag="SCK51_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1250843.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            21331..21753
                     /locus_tag="SCK51_000187"
     CDS             21331..21753
                     /locus_tag="SCK51_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1250844.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            21750..21980
                     /locus_tag="SCK51_000188"
     CDS             21750..21980
                     /locus_tag="SCK51_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1250845.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            22478..22678
                     /locus_tag="SCK51_000189"
     CDS             22478..22678
                     /locus_tag="SCK51_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG1250846.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            22705..23898
                     /locus_tag="SCK51_000190"
     CDS             22705..23898
                     /locus_tag="SCK51_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1250847.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(23966..25531)
                     /gene="guaA"
                     /locus_tag="SCK51_000191"
     CDS             complement(23966..25531)
                     /gene="guaA"
                     /locus_tag="SCK51_000191"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEG1250848.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25590..26513)
                     /locus_tag="SCK51_000192"
     CDS             complement(25590..26513)
                     /locus_tag="SCK51_000192"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEG1250849.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26709..27314
                     /locus_tag="SCK51_000193"
     CDS             26709..27314
                     /locus_tag="SCK51_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1250850.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27622..28638
                     /locus_tag="SCK51_000194"
     CDS             27622..28638
                     /locus_tag="SCK51_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG1250851.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            28779..30080
                     /locus_tag="SCK51_000195"
     CDS             28779..30080
                     /locus_tag="SCK51_000195"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEG1250852.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            30126..30788
                     /gene="deoC"
                     /locus_tag="SCK51_000196"
     CDS             30126..30788
                     /gene="deoC"
                     /locus_tag="SCK51_000196"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEG1250853.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            30809..31207
                     /locus_tag="SCK51_000197"
     CDS             30809..31207
                     /locus_tag="SCK51_000197"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEG1250854.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            31273..32355
                     /locus_tag="SCK51_000198"
     CDS             31273..32355
                     /locus_tag="SCK51_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1250855.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            32567..34129
                     /locus_tag="SCK51_000199"
     CDS             32567..34129
                     /locus_tag="SCK51_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1250856.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            34122..35255
                     /locus_tag="SCK51_000200"
     CDS             34122..35255
                     /locus_tag="SCK51_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1250857.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            35255..36211
                     /locus_tag="SCK51_000201"
     CDS             35255..36211
                     /locus_tag="SCK51_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1250858.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            36310..37476
                     /gene="deoB"
                     /locus_tag="SCK51_000202"
     CDS             36310..37476
                     /gene="deoB"
                     /locus_tag="SCK51_000202"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEG1250859.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            37500..38318
                     /locus_tag="SCK51_000203"
     CDS             37500..38318
                     /locus_tag="SCK51_000203"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEG1250860.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            38335..39042
                     /gene="deoD"
                     /locus_tag="SCK51_000204"
     CDS             38335..39042
                     /gene="deoD"
                     /locus_tag="SCK51_000204"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEG1250861.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(39340..40026)
                     /gene="gpmA"
                     /locus_tag="SCK51_000205"
     CDS             complement(39340..40026)
                     /gene="gpmA"
                     /locus_tag="SCK51_000205"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEG1250862.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(40198..40779)
                     /locus_tag="SCK51_000206"
     CDS             complement(40198..40779)
                     /locus_tag="SCK51_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1250863.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(40912..41595)
                     /gene="rpiA"
                     /locus_tag="SCK51_000207"
     CDS             complement(40912..41595)
                     /gene="rpiA"
                     /locus_tag="SCK51_000207"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEG1250864.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            41914..42327
                     /gene="rpsL"
                     /locus_tag="SCK51_000208"
     CDS             41914..42327
                     /gene="rpsL"
                     /locus_tag="SCK51_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEG1250865.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            42411..42881
                     /gene="rpsG"
                     /locus_tag="SCK51_000209"
     CDS             42411..42881
                     /gene="rpsG"
                     /locus_tag="SCK51_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEG1250866.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            42975..45062
                     /gene="fusA"
                     /locus_tag="SCK51_000210"
     CDS             42975..45062
                     /gene="fusA"
                     /locus_tag="SCK51_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG1250867.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            45234..46421
                     /gene="tuf"
                     /locus_tag="SCK51_000211"
     CDS             45234..46421
                     /gene="tuf"
                     /locus_tag="SCK51_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEG1250868.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            46828..47136
                     /gene="rpsJ"
                     /locus_tag="SCK51_000212"
     CDS             46828..47136
                     /gene="rpsJ"
                     /locus_tag="SCK51_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEG1250869.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            47167..47796
                     /gene="rplC"
                     /locus_tag="SCK51_000213"
     CDS             47167..47796
                     /gene="rplC"
                     /locus_tag="SCK51_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEG1250870.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            47824..48447
                     /gene="rplD"
                     /locus_tag="SCK51_000214"
     CDS             47824..48447
                     /gene="rplD"
                     /locus_tag="SCK51_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEG1250871.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            48447..48737
                     /gene="rplW"
                     /locus_tag="SCK51_000215"
     CDS             48447..48737
                     /gene="rplW"
                     /locus_tag="SCK51_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEG1250872.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            48776..49609
                     /gene="rplB"
                     /locus_tag="SCK51_000216"
     CDS             48776..49609
                     /gene="rplB"
                     /locus_tag="SCK51_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEG1250873.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            49652..49930
                     /gene="rpsS"
                     /locus_tag="SCK51_000217"
     CDS             49652..49930
                     /gene="rpsS"
                     /locus_tag="SCK51_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEG1250874.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            49953..50300
                     /gene="rplV"
                     /locus_tag="SCK51_000218"
     CDS             49953..50300
                     /gene="rplV"
                     /locus_tag="SCK51_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEG1250875.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            50314..50970
                     /gene="rpsC"
                     /locus_tag="SCK51_000219"
     CDS             50314..50970
                     /gene="rpsC"
                     /locus_tag="SCK51_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEG1250876.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            50973..51407
                     /gene="rplP"
                     /locus_tag="SCK51_000220"
     CDS             50973..51407
                     /gene="rplP"
                     /locus_tag="SCK51_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEG1250877.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            51397..51585
                     /gene="rpmC"
                     /locus_tag="SCK51_000221"
     CDS             51397..51585
                     /gene="rpmC"
                     /locus_tag="SCK51_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEG1250878.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            51610..51876
                     /gene="rpsQ"
                     /locus_tag="SCK51_000222"
     CDS             51610..51876
                     /gene="rpsQ"
                     /locus_tag="SCK51_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEG1250879.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            51934..52302
                     /gene="rplN"
                     /locus_tag="SCK51_000223"
     CDS             51934..52302
                     /gene="rplN"
                     /locus_tag="SCK51_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEG1250880.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            52339..52647
                     /gene="rplX"
                     /locus_tag="SCK51_000224"
     CDS             52339..52647
                     /gene="rplX"
                     /locus_tag="SCK51_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEG1250881.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            52675..53214
                     /gene="rplE"
                     /locus_tag="SCK51_000225"
     CDS             52675..53214
                     /gene="rplE"
                     /locus_tag="SCK51_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEG1250882.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            53233..53418
                     /locus_tag="SCK51_000226"
     CDS             53233..53418
                     /locus_tag="SCK51_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEG1250883.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            53454..53852
                     /gene="rpsH"
                     /locus_tag="SCK51_000227"
     CDS             53454..53852
                     /gene="rpsH"
                     /locus_tag="SCK51_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEG1250884.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            53884..54420
                     /gene="rplF"
                     /locus_tag="SCK51_000228"
     CDS             53884..54420
                     /gene="rplF"
                     /locus_tag="SCK51_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEG1250885.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            54581..54937
                     /gene="rplR"
                     /locus_tag="SCK51_000229"
     CDS             54581..54937
                     /gene="rplR"
                     /locus_tag="SCK51_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEG1250886.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            54958..55458
                     /gene="rpsE"
                     /locus_tag="SCK51_000230"
     CDS             54958..55458
                     /gene="rpsE"
                     /locus_tag="SCK51_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEG1250887.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            55473..55652
                     /gene="rpmD"
                     /locus_tag="SCK51_000231"
     CDS             55473..55652
                     /gene="rpmD"
                     /locus_tag="SCK51_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEG1250888.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            55696..56136
                     /gene="rplO"
                     /locus_tag="SCK51_000232"
     CDS             55696..56136
                     /gene="rplO"
                     /locus_tag="SCK51_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEG1250889.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            56136..57431
                     /gene="secY"
                     /locus_tag="SCK51_000233"
     CDS             56136..57431
                     /gene="secY"
                     /locus_tag="SCK51_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEG1250890.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            57493..58140
                     /locus_tag="SCK51_000234"
     CDS             57493..58140
                     /locus_tag="SCK51_000234"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEG1250891.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            58331..58549
                     /gene="infA"
                     /locus_tag="SCK51_000235"
     CDS             58331..58549
                     /gene="infA"
                     /locus_tag="SCK51_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEG1250892.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            58582..58698
                     /gene="rpmJ"
                     /locus_tag="SCK51_000236"
     CDS             58582..58698
                     /gene="rpmJ"
                     /locus_tag="SCK51_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEG1250893.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            58716..59081
                     /gene="rpsM"
                     /locus_tag="SCK51_000237"
     CDS             58716..59081
                     /gene="rpsM"
                     /locus_tag="SCK51_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEG1250894.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            59109..59498
                     /gene="rpsK"
                     /locus_tag="SCK51_000238"
     CDS             59109..59498
                     /gene="rpsK"
                     /locus_tag="SCK51_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEG1250895.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            59578..60516
                     /locus_tag="SCK51_000239"
     CDS             59578..60516
                     /locus_tag="SCK51_000239"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEG1250896.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            60544..60924
                     /gene="rplQ"
                     /locus_tag="SCK51_000240"
     CDS             60544..60924
                     /gene="rplQ"
                     /locus_tag="SCK51_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEG1250897.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            61196..62035
                     /locus_tag="SCK51_000241"
     CDS             61196..62035
                     /locus_tag="SCK51_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1250898.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            62011..62880
                     /locus_tag="SCK51_000242"
     CDS             62011..62880
                     /locus_tag="SCK51_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1250899.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            62870..63667
                     /locus_tag="SCK51_000243"
     CDS             62870..63667
                     /locus_tag="SCK51_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEG1250900.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            63870..64616
                     /gene="truA"
                     /locus_tag="SCK51_000244"
     CDS             63870..64616
                     /gene="truA"
                     /locus_tag="SCK51_000244"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEG1250901.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(64949..67618)
                     /locus_tag="SCK51_000245"
     CDS             complement(64949..67618)
                     /locus_tag="SCK51_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEG1250902.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(67856..68044)
                     /locus_tag="SCK51_000246"
     CDS             complement(67856..68044)
                     /locus_tag="SCK51_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEG1250903.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            68314..68676
                     /locus_tag="SCK51_000247"
     CDS             68314..68676
                     /locus_tag="SCK51_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1250904.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            68728..70410
                     /locus_tag="SCK51_000248"
     CDS             68728..70410
                     /locus_tag="SCK51_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEG1250905.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            70527..72563
                     /gene="recG"
                     /locus_tag="SCK51_000249"
     CDS             70527..72563
                     /gene="recG"
                     /locus_tag="SCK51_000249"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEG1250906.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            72612..73613
                     /gene="plsX"
                     /locus_tag="SCK51_000250"
     CDS             72612..73613
                     /gene="plsX"
                     /locus_tag="SCK51_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEG1250907.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            73735..73977
                     /gene="acpP"
                     /locus_tag="SCK51_000251"
     CDS             73735..73977
                     /gene="acpP"
                     /locus_tag="SCK51_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEG1250908.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            74245..75249
                     /locus_tag="SCK51_000252"
     CDS             74245..75249
                     /locus_tag="SCK51_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1250909.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            75250..76194
                     /locus_tag="SCK51_000253"
     CDS             75250..76194
                     /locus_tag="SCK51_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1250910.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            76194..77156
                     /locus_tag="SCK51_000254"
     CDS             76194..77156
                     /locus_tag="SCK51_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1250911.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            77183..78097
                     /locus_tag="SCK51_000255"
     CDS             77183..78097
                     /locus_tag="SCK51_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1250912.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            78123..79904
                     /locus_tag="SCK51_000256"
     CDS             78123..79904
                     /locus_tag="SCK51_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1250913.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            80252..80938
                     /locus_tag="SCK51_000257"
     CDS             80252..80938
                     /locus_tag="SCK51_000257"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEG1250914.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            80958..84539
                     /gene="smc"
                     /locus_tag="SCK51_000258"
     CDS             80958..84539
                     /gene="smc"
                     /locus_tag="SCK51_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEG1250915.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            84548..85357
                     /locus_tag="SCK51_000259"
     CDS             84548..85357
                     /locus_tag="SCK51_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1250916.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            85368..86366
                     /gene="ftsY"
                     /locus_tag="SCK51_000260"
     CDS             85368..86366
                     /gene="ftsY"
                     /locus_tag="SCK51_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEG1250917.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            86553..88820
                     /gene="gshAB"
                     /locus_tag="SCK51_000261"
     CDS             86553..88820
                     /gene="gshAB"
                     /locus_tag="SCK51_000261"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEG1250918.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(88909..89361)
                     /locus_tag="SCK51_000262"
     CDS             complement(88909..89361)
                     /locus_tag="SCK51_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEG1250919.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            89511..90461
                     /locus_tag="SCK51_000263"
     CDS             89511..90461
                     /locus_tag="SCK51_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEG1250920.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            90454..91413
                     /locus_tag="SCK51_000264"
     CDS             90454..91413
                     /locus_tag="SCK51_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEG1250921.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            91410..92165
                     /locus_tag="SCK51_000265"
     CDS             91410..92165
                     /locus_tag="SCK51_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1250922.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            92188..93141
                     /locus_tag="SCK51_000266"
     CDS             92188..93141
                     /locus_tag="SCK51_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1250923.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
BASE COUNT        30825 a        16622 c        19722 g        26402 t
ORIGIN      
        1 attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag atttattaca
       61 gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa
      121 atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat
      181 ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt
      241 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
      301 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      361 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      421 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      481 ggtgggttat tttacgagta aaggagactt gctttatccc actcaattat tagataaaat
      541 agaatttgat tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat
      601 cggacgctca gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg
      661 aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata
      721 agattcattc aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca
      781 ttcccaatat ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac
      841 aacgtcgaat aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga
      901 ttcaacgagc agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg
      961 aatccgacga aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg
     1021 gcaatcgagg atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa
     1081 ggaaaggtga aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt
     1141 tggcacattg aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac
     1201 ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat
     1261 gatacaagtc agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt
     1321 ggaattaatc aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt
     1381 tgattggtac gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa
     1441 atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt
     1501 gattttgaaa gaaattccga aggatgaagc catttctttt attcggcaat accattattc
     1561 aaagatactc cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt
     1621 aggcgttgtg gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc
     1681 cgacagttcc cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga
     1741 aatgaatcaa accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa
     1801 agaacatacg gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg
     1861 ctatgtctat caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg
     1921 tgataagcag tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa
     1981 tgctcgcttt gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa
     2041 agggattgag aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc
     2101 gaaaaaactg ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt
     2161 acgctttgaa aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga
     2221 taaacaggct cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat
     2281 gccattgctt catgacagag gagagcgaat caaagccaac gataagcact tggtctatca
     2341 ttttgtacta atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag
     2401 agcgttacaa tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca
     2461 tacgccatac ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg
     2521 gctttattat cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc
     2581 tcgtatgatt ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt
     2641 taaagattta cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta
     2701 tcgaatgaaa gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga
     2761 tcagcttttg cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa
     2821 aacgttgaaa gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat
     2881 tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg
     2941 ggagttcgat tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta
     3001 ttttattctc acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga
     3061 ccctaaaaat gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa
     3121 agaagatatg ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac
     3181 catgaaacaa caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc
     3241 caattttctt atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac
     3301 caccgttatt cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt
     3361 aattcttgcc tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt
     3421 taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag
     3481 cgatacacaa aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg
     3541 aaaaagtgtg attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg
     3601 agaaatagga aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg
     3661 tacaggttca gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct
     3721 ctatctaaca ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg
     3781 atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc
     3841 ttttttagat tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag
     3901 aaaagcgact ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca
     3961 gaccttacct atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag
     4021 atttactgaa cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact
     4081 atgtacggat tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt
     4141 tgaatatgga ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc
     4201 gtttcggtga tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac
     4261 tgaaaggaaa aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt
     4321 atgatttctt tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta
     4381 tcaacgataa aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag
     4441 aatgcatttc cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg
     4501 atgaaaaacc atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt
     4561 tctgtatata tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg
     4621 cagaagtaaa gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga
     4681 aagatttatt ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata
     4741 tccgctttgc agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt
     4801 gggaatggtt tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt
     4861 attcttttga acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg
     4921 caagcatttt agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca
     4981 aactgacaga aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga
     5041 ttacatgagc cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt
     5101 tcaagcgtat atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca
     5161 agctggtgat accatctata cctatgattt ttacaaggtg agattttata ataattatac
     5221 caatcgagta accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa
     5281 aagctatatc tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt
     5341 gggcagaatt tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt
     5401 gttgtcattg gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg
     5461 attattgctc taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta
     5521 ttaaacttat ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa
     5581 gtatatattt tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc
     5641 ttttaattaa aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga
     5701 ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt
     5761 ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt
     5821 ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca
     5881 tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga
     5941 attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg
     6001 aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca
     6061 ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag
     6121 acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac
     6181 ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct
     6241 ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa
     6301 agagagaagc aaaactaata ataggtattt caagtacagt tacagattat attagcggat
     6361 accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa
     6421 ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag
     6481 gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa
     6541 tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg
     6601 tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg
     6661 aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg
     6721 aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag
     6781 atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac
     6841 aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag
     6901 ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa
     6961 attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta
     7021 ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa
     7081 cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt tccagaatat
     7141 gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct agctgtctat
     7201 gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt tggtatcgaa
     7261 tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc tcgatttatt
     7321 ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta
     7381 agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag aagaaatcct
     7441 ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa aacgagattg
     7501 tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt cagatattca
     7561 atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac aaaaaataaa
     7621 attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt tttctatttt
     7681 tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt tggtcagtag
     7741 aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc tttacgcaaa
     7801 tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta ccaccacttt
     7861 ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc actgggttca
     7921 tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct atcattacct
     7981 atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa aaagaaacaa
     8041 tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc gattaaatat
     8101 atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta tgaattaatt
     8161 ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga taatttctgt
     8221 caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc aaccgaatca
     8281 agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt aaaagagata
     8341 gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg tgacaatcaa
     8401 gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga agtatccgta
     8461 aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac
     8521 aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt ttctaagatg
     8581 gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt ggagaaagat
     8641 gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt tgatgactac
     8701 gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa atacgatatt
     8761 ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat tgaacaggaa
     8821 gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga attggatttc
     8881 cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt tgataccagt
     8941 atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa
     9001 gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg
     9061 gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac caaagaatct
     9121 atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga gctgaaacaa
     9181 cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt acgacctttt
     9241 ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata tatcaatgac
     9301 tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc tacacaaatg
     9361 attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag aaatatttat
     9421 ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc attagcgtcc
     9481 gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct ggtctattat
     9541 tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg
     9601 aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac gagtgagaac
     9661 gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga ttcagaaagc
     9721 cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag cgagaagttt
     9781 cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg gttgttattg
     9841 gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc tgaccatatc
     9901 gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag tgtcacccaa
     9961 tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt cttacccgat
    10021 tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc aatgctgatt
    10081 gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt taaaattgtt
    10141 gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct atctatgcga
    10201 ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca aaacgcggac
    10261 gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt tcgttccact
    10321 gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga agatgaaaac
    10381 aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga catctatggt
    10441 cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc ctttgatacc
    10501 agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact
    10561 atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc acgctgggca
    10621 atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat gaatactcca
    10681 agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg ggctggcttc
    10741 catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat gccattacta
    10801 attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg gtgaaagaag
    10861 cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac atgcagacga
    10921 ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt ttcctccttc
    10981 tttttattct cattgttgga atctatgttg cctatacagg attggttaag cgagaaacga
    11041 cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg gcttccttta
    11101 ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg
    11161 ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa gggaaagaca
    11221 gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca tggctattgc
    11281 ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa agtttattgt
    11341 ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt gaagaaattg
    11401 aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta ggaacagtct
    11461 tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta acaggaatca
    11521 tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc agtttcctat
    11581 taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag ttatttaatg
    11641 tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt atttccacta
    11701 tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta caaattgtga
    11761 cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact ttacaaagca
    11821 acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac
    11881 aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca gggtcaacga
    11941 agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat actcgtcaaa
    12001 atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag caacaaggcg
    12061 gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa aatcgtctag
    12121 tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat gtcaaatatg
    12181 cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa caatctatgg
    12241 cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga
    12301 acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga aaagaaaacc
    12361 catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc
    12421 ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa
    12481 aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga acggataagc
    12541 gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct tctctctctt
    12601 cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca gtggcgaaaa
    12661 caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca
    12721 ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg tttcttatat
    12781 cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc aaagttccga
    12841 atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac aaggggttaa
    12901 atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag attctgttat
    12961 ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg ggaaaaaata
    13021 tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa aagcagacta
    13081 tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg gaaatcagtt
    13141 ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat ttgatgatga
    13201 aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct atgtctttgg
    13261 aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg tgtatgacaa
    13321 agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc aagaaatctc
    13381 tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata atgcaggcac
    13441 atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg caggcgaccc
    13501 aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg gggctagacg
    13561 tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa
    13621 agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta
    13681 aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg
    13741 gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa
    13801 tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga
    13861 aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga
    13921 cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc
    13981 ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca
    14041 cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta
    14101 tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca
    14161 aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa
    14221 gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct
    14281 tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt
    14341 tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa
    14401 aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa
    14461 cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat
    14521 aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa
    14581 ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg
    14641 agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa
    14701 ataattagat gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt
    14761 tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg
    14821 cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt
    14881 tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgca
    14941 ggaaaaacta ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga
    15001 agcgtggaca aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt
    15061 acaattcaga caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac
    15121 acgccaggac atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg
    15181 gcaattctac tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat
    15241 gcacttagga aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga
    15301 attgatttat caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc
    15361 aaacagaagg tagaactgta tcctaatatg tgtgtgacga actttaccga atctgaacaa
    15421 tgggatacgg taatagaggg aaacgatgac cttttagaga aatatatgtc cggtaaatca
    15481 ttagaagcat tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc
    15541 cctctttatc atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt
    15601 actaataaat tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc
    15661 aaaattgaat atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt
    15721 acaaaaagtg aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt
    15781 tttttattgt taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt
    15841 atataaaaat gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg
    15901 ttacaattta ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat
    15961 aagaacgcta agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa
    16021 tacgctgaat atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt
    16081 ttagacgata agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt
    16141 gaaaaaatgg gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta
    16201 gccacgaagg aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa
    16261 gatagggcta aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa
    16321 atgttgttct tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat
    16381 tactgcatca atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta
    16441 aaagggaaac caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt
    16501 atgtactgca atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct
    16561 ttatctaatt ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa
    16621 gatgttgagc cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca
    16681 ttcaatgagc cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat
    16741 caatgtaagg cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact
    16801 tatcaccaac aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt
    16861 tcagtcagtc gtacttgtga tattttagga attggagttg gaacctacta tcataagctg
    16921 gagtggctgt ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa
    16981 acaaagaaat ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac
    17041 gtccgtaaga aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa
    17101 acgtatatag ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc
    17161 tatgattgga atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac
    17221 catctcaata cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa
    17281 ccatccgcta acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact
    17341 aataggagta gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac
    17401 tattggttaa ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac
    17461 ttctctatta gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca
    17521 catcatttta tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc
    17581 ggacaagcaa aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt
    17641 ctacggactt tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa
    17701 gaggctatta gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat
    17761 ccattagcca ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct
    17821 ctggaaccaa acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc
    17881 tttattcaac agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt
    17941 ggtgataaga agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt
    18001 acaattctaa gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt
    18061 ttgacacctg ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt
    18121 tatttcaaat gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg
    18181 ttcttcttca attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa
    18241 tagtagttat acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat
    18301 tcctcataat tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg
    18361 atgtacattt tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata
    18421 aaaatttctt cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca
    18481 caaccattaa aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag
    18541 gctcttccga ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta
    18601 tttttaacat attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc
    18661 actctattct tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta
    18721 actaatttac catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg
    18781 ttagttgaat aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa
    18841 ttttcattca attatcattt tcaattgcat tttataaatc attttgtatg taatttggta
    18901 tgtaagttaa aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca
    18961 aaactgaact acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat
    19021 tcggttagag tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat
    19081 ggtgaattat gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag
    19141 tttttgaagt taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt
    19201 gaaaatccgc accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa
    19261 atgttgcttg atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg
    19321 gattctacga cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt
    19381 agtgcactgt tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt
    19441 tatatggaga gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat
    19501 cctttctggg cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag
    19561 tatgagagct cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa
    19621 gggatacgct atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc
    19681 tgttttaagt acggtttata ctatagccct gttagtactc cagcagattt tcggatgctt
    19741 actcctattg tactggagca agcctttaga aaagctggaa cagaattgtt agagccatat
    19801 cttagtttta aagtttatgc accacaggaa tatctttcac gggcatataa cgatgctccc
    19861 aaatattgtg caaatatcgt aaatactcaa ctgaaaaata atgaggtcat tattattgga
    19921 gaaattcctg ctcgatgtat tcaagattat cgcaatgatt taactttttt tacaaatggg
    19981 cttagtgttt gtttagcaga gctaaaagga tatcaggtta ccactggcga acctgtttgc
    20041 cagacccgtc gtctaaatag tcggatagat aaagtaagat atatgttcaa taaaataact
    20101 tagtgcgttt tatgttgtta tataaatatg gtttcttatt aaataagatg aaatattctt
    20161 taatatagat ttgaattaaa gtggaaagga ggagattgtt attataaact acaagtggat
    20221 attgtgtcct atttgtggaa ataaaacaag actacgaata cgagtggata ctatacttaa
    20281 aaatttccct ttatactgcc ccaaatgtaa gaacgaaact ttaattaatg ttcaaaaaat
    20341 gaatataata acaatcaaag agccagacgc caagacgcag agccgataat ttgagaaatg
    20401 aaactctcat cttatcggct ctttttgttt atctgaattt tactgactag ccttcaatat
    20461 ttcttgcttc attgatacct tttgctagtg cttccattaa ggatagttct ttgtctgtaa
    20521 agctatccat gtatttttct acctgtaacc ttcgagtgct tttaatcaaa ttatctgtag
    20581 gtaagaaaaa ttcatcaata gatacatgga gtaatgatac aagatcatac agcacttgtg
    20641 tacttgggtg ttgtcctttg ttttctatat ttgttaaata acgtgggtca atttcaatca
    20701 ttgttcccac ttgttcacga gtgagacccc gtttcattcg agcctcttta atcgctaaac
    20761 caacggctct aaaatcgtat ttatcttctt ttttacgcat actaaatcac ctctatacat
    20821 tttactgttc ctatataacc ataaacaggt atagaaaaac atgttatata gtttatgagt
    20881 tcctatagaa cactgtggaa tttattaatg cgatgaatcc aatttcaaat agaaaggtcg
    20941 ctgattttat ttacctatac aactttataa aatttagtca tactcctaga aagtagataa
    21001 aattggcgac ttaatggagg gaaaagtcgc catgagaaag ataatctgtc atctagttct
    21061 cttaattaag ttcttatcaa aaaaacttat ccacctgtac tggatagata tgaccatcaa
    21121 tgtggttttg cagattacat agatagaaaa atcaaagcat atgaacagag aaaacaatct
    21181 gtttatgtgc ttttttggtc acttaataaa tatcttttag gatttctctt catttatgca
    21241 acaaatcccc cgttcgtgtc ggggtttagt atgaagggag ataaagaagc acatgccact
    21301 ccctttcacg acagaaaggg ggtgaaaagc atgaaaccat cttcttttca agaaaccata
    21361 gaaaatcagt ttgactatat ctgcaaacaa gttattgaag atgaacgaaa agattacttc
    21421 aaacatctag acagactatc agcaaaagaa gtctcgtttt ctgatataga taactacctt
    21481 gtcaactctt tttctactgt tgatacgtac gtcactgatt tccaactatt taaattattt
    21541 ggattaagaa tcaatgttga aagtgattta ttaagtgaag ccctgcgtca tctttcagag
    21601 aaaaaacgga acattatttt acttcattac tttatggata tgagtgatgt ggagattgcg
    21661 gagttactaa atttgaatcg ttcgactgtt tatcgtcatc gaataagtgg actagccatg
    21721 attaaagaat tcatgaagga gtgtaaagaa tgaaaacagc ttatccaagg gttcccttcc
    21781 ccctcattgt aaaggcgaca gatggcgacg tggaagcaat taatcagatt gttaaacatt
    21841 atcgagggta tacctcaaaa cgttcattac gtcgcatgac agacgaatat ggaaattctc
    21901 atatggttat tgatgaaacc ctacgtggca gaatggaaac gaaactcatc tctaagattt
    21961 tatcgttcga aattaaatag tcccattttt tcgtggaagc gtgtcgaatc gtatccacgc
    22021 ttcccgattt gggaggtttg ttattccatc aaagcatatt gtgtgttcag taggttttga
    22081 taggctaaca agccaaacgt tctttgacaa ccaaataaag gcaatcgaat acgtttcggt
    22141 aagaaaagag ctgatgaact aacacgccat gacctacaaa attagtgagc gattcatgtt
    22201 agtaattcga aagtaatctt tagcaggatt gctgccatga ctttcttatc gtgataatga
    22261 tactcccgta tagccatagt ccgagcgtta gaagcgtcac aggcaatggg tacggctaca
    22321 tgagaaccat gcagaagtga aattcctgtg agctttgcta gagctgttcg attgctaaga
    22381 tattcttctc ttgtcagtgt attattataa aactatttga tataattata ggtgaatatt
    22441 ggagttttcc tagcctataa ttgggaaagg agtaataatg ataacggtca caaaaaagga
    22501 tttagttgag ctaggatatg gaacttcttt ttcagcagac atcataaaaa aagcaaaaga
    22561 attgatgatt acaaaagggc atatctacta tcaatctcga aagctagata gagtccctcg
    22621 tgaagctgtt gaagaaattc taggaattcg gttttcaaat gaaaacaggt ctgagtagtt
    22681 tttgcacagt aaggagtaaa aactatggtt aaagacccaa taaaaaaagc aaagaatgga
    22741 acctattatt ttcgtgcaaa tctaggttat gattctaaag ggaaaaaaat tcaaaaatat
    22801 cgtagtggat tcactactaa aaaagaagca agagaagaat attcgaaact gctattgatg
    22861 aaaccagaag aattaagtga gaataaggat aaaatgacgt ttaaacatta tatctttgaa
    22921 attttccttc catggtataa gacacaggta aagctcagaa catatgaaaa tcgcttacca
    22981 actattaaga agcatttctc ttattttgat aaaatggctg tttccgatat tgagcctatt
    23041 gatgtgcaaa attggcaact aaaattatca aagaagtgta agtcttctta tgtaagagct
    23101 gttcaaggac tattttcagt tgctatggat cgagcaattg tccttgggat aaccaccaca
    23161 aatccatcta agattattgg caatgtgaaa aagcaaaagt ctaaaattga attttggaca
    23221 aaagaagaat ttgaaaaagt aatctctcta atttacaaag aagattacta tcaacacttt
    23281 ttatttatct ctctgtggtt tttatttatg actggaatgc gaattggtga agctactgcc
    23341 attcaatggg aagatattga ttttgactca ggagtactaa ccatcaacaa aacactctat
    23401 tacaagaatc aatcgaatta tagatttgtt gaaccaaaaa caaaggcgag cgttcgtcat
    23461 attgtattag ataaatgcac attaacttat ttatccgaat ggaaggaagt acaacaaaat
    23521 ctaattcaga cagactttgt aatgagctat aacggtattc ctacccaaaa acatacacta
    23581 tcatatgcta ttgaacgcta tgctaagctg gctggtatcc atcgaatcag aatccatggg
    23641 ttacggcatt ctcatgcttc cttgttaatc agtatgggag aaaatccact tatcattaaa
    23701 gatcgtctag gacacgaaga tattgagacg actttaggaa cgtatggaca tctttatcct
    23761 aacagtaatt tcgaggtagc taacaaatta aatggagcca tttcctttaa agaagcaaaa
    23821 caaaattatg atacttcacc aaagaatcaa tttactgtag actacttgcg aaacaaagaa
    23881 atgaaaaaag tgcaataaaa gtgcagtaaa aagataagaa gagccagaaa cccttggtat
    23941 aagaggattt ctagctctcc tacaactatt cccactccac tgttgctggt ggtttagaag
    24001 tgatgtcata cacgatacgg ttgacgtggg ctacttcgtt tacgatacgt acagagattt
    24061 tttgcaagac ttcccatggg atacgggcaa agtcagcggt cattccgtcg attgaagtta
    24121 cagcacggat accgactgtg tagtcgtaag tacgtccgtc acccataaca ccgacagagc
    24181 ggatccctgg aagaacagtg aaatattgcc agatatcacg gtcaagtcca gcagctgcga
    24241 tttcttcacg caagatagca tcagattcgc gtacgatttc tagcttgtct tcagtgattt
    24301 cgccaagaac acgaatacca agaccaggac cagggaatgg ctgacgccaa acgattgaat
    24361 caggcatgcc taactgagtc cctaattcac ggacttcgtc tttgaataat gtgtttaatg
    24421 gttcgatcaa ttcaaattgc atatcttctg gcagtccacc tacattatgg tgagatttga
    24481 tcgtctgtgc tgtttctgta ccagattcga taacgtcagt atacaacgtt ccttgtgcta
    24541 ggaaagagac gccttcttca ccagcaagtt ttgttgcttc gtcatcaaaa acatatacga
    24601 attcattccc aatgatttta cgtttttgtt ctgggtcaga tacacctttc aacttgtcta
    24661 agaagcgatc tcttgcatcg actttgatga tgttcaagcc gaattttccg cctagacttt
    24721 ccattacttg ttccgcttcg cctttgcgta gcagaccgtg gtcaacaaag atagatgtca
    24781 attggtcacc gatcgctttt tgtaaaagaa ccccaacgac actagaatca acgccacctg
    24841 aaagaccaag caagactttt ttgtcaccaa cttgttcacg gattttttct acttgcatat
    24901 cgataaagtt atccattgtc cagttacctt cacagtgaca aacatcaaag gcaaagtggc
    24961 gcagcaattc gattccgtat tcggaatgac gtacttctgc atggaattgg ataccataaa
    25021 aattacgtgt tgtgtcttgg atagaagcaa tcggacaatc tttgcttgtt ccgactgttt
    25081 caaagccttc aggaacttta gttaccaagt caccatggct catccatact gtttgttgtt
    25141 ttggtgtttc tttaaacaag acagcttgat cgcttgttac ttctaacaag gctttcccat
    25201 attcgcggtt tttagcaggc tctacggaac cgccaagttt gtacgtgatc aattgcatac
    25261 catagcaaat ccctaataca ggaataccta aattgaagat ctcaggatcg atcccaaatg
    25321 cattctcatc atatacactg tttggtccgc cagaaagaat gattcccttt ggattcatcg
    25381 cttttacttc ttctgcagta atgcggtggc tcaataattc ggaaaagact ccaagatcac
    25441 gaatacgtct tgtgatcaat tggttatact gactaccgta gtctaatacg ataatctttt
    25501 caacgttcgt catatcggca acgtttgtca aatttattac ccctatccct ttaaatttta
    25561 tctcaaaata gtcaaactat tttaaaatgc taatattttc gcatgaaaat cgaatcgatg
    25621 atatgatttt cagttttatg gagaattata tcggctcttc ctcgtgtagg caagatatat
    25681 tcctgtaaat ttttcaaatt cacatttttc catacatctt ttgccatttt aaaggcttct
    25741 tcacgatctc cgatagcaaa acgataataa taattctctg gattttggaa agccgtatcc
    25801 agtaacgcac caaaacgatc caaataccat ttttcgatca gcacgggatc ggcatcgaca
    25861 tagacagaaa aatcaaaaaa gtcgctgacg tagatttgct gattcgcagg taattgaagc
    25921 gtattgatcc cctcaacgat caagatatca ggctgctgga tcaattcgta cttgtctgga
    25981 atgatatcat acacatcatg tgagtaaaca ggtactttta tctcttcctg accagatttt
    26041 acacggttca agaaatcgat cagcatctcc atatcataac tttcaggaaa tcctttacgg
    26101 tccatgattc cacgctcttt taatatacga tttggataaa gaaatccgtc agtcgtgatc
    26161 agctgaacgt ttcttcgttt gaaggtacga gacaaaatcg tctgcagcag tctggctgtt
    26221 gtactttttc caacggctac acttccggcg atcccgataa taaaaggcgg tacttctacg
    26281 tatttatgaa gaaataaacc tttactcaag aacaaagatt caaattcttt catataaaga
    26341 taaatcagat gagcaagagg tacatagata tctcgaacat cctgcatgga aatgcggtca
    26401 ttcaaacttt tgatattatc tagttcatct tgtgtcaaag gtgcttgccc attacgataa
    26461 aattctcgcc attcttctct tgccacacgg tagtagttca ttggatcatt catattagcc
    26521 tcctgcgttc tcccatcaat ggaactattt taacacaaaa taaacgcaaa ccccaccact
    26581 taagagagat tgccgatctt tttgtataat ctttataata gaaagaaacg gagtcttatt
    26641 cttgacttgc gcacggatac ataaaaggat agactgttgt catgaaagaa atgagaagga
    26701 tggaagacat gacaaatcat tactattctg aacgcccaga tacggcgcac gattttgaac
    26761 aatgggcttt tgagttaaaa ggcagaacct ttcaatttgt aacagattct ggtgtatttt
    26821 cacgtgaaac agtggattat ggttcacgtg tcctgattga tgctttcgat tgggaagagt
    26881 tacctgatga aggaaagatc ttagatgtgg gatgcgggta tggaccgatt gggttagctt
    26941 tggcatttgc gacacagcgg tttgttgaaa tggtcgacat taatgaacga gcggtaagtc
    27001 tagcgcaagg aaacgcaaag cgtaaccaaa tcgaacatgt tgatatccat caatcgaata
    27061 tctatgaagc agttcatgaa gaaacctatg cagctatcgt aagcaatccg ccgatccgtg
    27121 ctggaaaaaa agttgtacat gaaattttga caggcgcata tcctcggttg aagaagggcg
    27181 gtacactgac aattgtgatc cagaagaaac aaggagcgcc gagtgcgcaa aagaaaatgg
    27241 aagaaacatt tggtaatgca gaaatcgtca caaaagataa aggatattat atcatcaaaa
    27301 gtgtgaaaga ataactagct gaaaaatagt ggagcgacga aagagtggaa ctgggcgtgc
    27361 atgtacgggt acgtatcagt tctttttttg ttgagaagtt tttaaaaaca aagaactatt
    27421 ttttcttcaa aaagaaatag taggaacaga gtcgttgttt tttacgataa aataaataag
    27481 atacagaact tgtatagaag gctgccaagt cggtagataa aggaggtttt tatcaaaaaa
    27541 catactttcc ttttttctct attcttttct tgggggtttt ggtacactga tagggaattt
    27601 agatagaagg gagccaatag gatgaagctg accatcagag atatcgcaga aatggcaggc
    27661 gtatcagtaa caactgtctc acagattttg aataacaaag gaagcagatt tagtgataag
    27721 acaagaaaaa aagtgttgtc gatcgtgaat gaacatcatt ataagcccga ttatttcgct
    27781 tcaaatgtga tcaatcgaca ttcaaagacc atcggaatga tcgtccctga tgtcactgat
    27841 ttcttcttct ccaaactgat cgaaggtgta gagagctatt tgaatccttt gggctatgtg
    27901 atcatgttat gcaattctcg tcatagccaa gaaaatgaga tcaagtattt acaagagctt
    27961 tcccaccgct cagtggacgg aatcttgctg gccacaccaa atattttacc tgagaaatat
    28021 gcattagaca gcggttttta tcaaaaaatg ccaatcattt taatcgatcg cggattgaat
    28081 cgtcgggata acggacggct tatcgtaaag gaatatgagg gcgcctatca agctgtttcg
    28141 ctgtttattg aaaatggcca tacgaagatc ggcatgctga aggaaaccac tggatactat
    28201 caattagaag aacgtttcaa cggatatcgt catgcgctga aagatcatgg cctgcctttt
    28261 aatgggaaat ttgttgaaca tggagagttg actgtccaag gtggatatac tgcatcaaaa
    28321 aaattactca aacataaaga tatcacggct attttttgcg ggaatgatgc catggcgatc
    28381 ggatgctatc aggcgatcga tgaattgggc aaaaaaatcc cagaagatat ctcagtcata
    28441 ggattcgatg gattaaagct gtcagaatac atgattccgc aattgacgac agtgcagcag
    28501 ccaagttttg atattggatt ttatgctgca cgtttcctga tcgatacgat cgaatttcct
    28561 caaagaaaag ttccgaacaa agtgttcgaa acaaaattaa ttattcgtga gagcgtaaaa
    28621 gtattgacaa agggctaact cggcggtata ttgcttatgt aagcatttac aaaggctagt
    28681 tagtgctttt tttattttta gtaaaccagt ttactaaagt ggtttacctt tcctaaaaat
    28741 aatgtataat accgaatgaa gacaaggagg atcaggcaat gcgaatggtg gatctgattg
    28801 aaaaaaagag ggatggaaac gagctgtcga aagaggaaat cgagtacatc gtcacgaact
    28861 atacgaatgg aaagattcca gattatcaag tcagtgcatt attgatggca attttttatc
    28921 aagacatgac aaatgaagaa atcacgaact taacattggc gattgctaat tctggtgatg
    28981 tcatcgattt gagttctctt gaaggaataa aggtagataa gcattcgaca ggtggcgttg
    29041 gtgacacgac cacattgatt ttagcccccc tagtagctag tgtcggagtg acagtggcta
    29101 aaatgtctgg acgcggtcta ggttacacag gtggtacact agacaagcta gaagcaattc
    29161 ccggatttca gatcgagctt tcggatgaag aatttgtacg cattgttaat gaaagtaaag
    29221 tagcggtaat tggtcaatca ggaaacttgg caccggcgga caaaaaattg tatgccttgc
    29281 gtgacgtcac agcgaccgtt gattctcttc cgctgattgc cagttcgatt atgagtaaaa
    29341 aaatcgcggc aggagcagat gcaatcgttt tggatgtgac cactggagac ggtgctttca
    29401 tgaagaacat cgaagatgca agacgtctgg caaagaccat gacgagcatt gggaaactgg
    29461 ctaaccgaga aacagtggcg gttatctcag atatgtcaga acctcttggc gaagctatcg
    29521 gaaatagtct agaagttgtc gaggcaatcg aaacattaca aggaaatggt ccagaagatc
    29581 tagtagaaat gtgctacgcc ttaggaagcc aaatggttgt tcttgctggc aaagctaaga
    29641 caaccgatga agcaagaacg ttgcttcaag aagctctgga atcaggtaaa gcgttagcaa
    29701 aattcaaaga aatgatccaa aatcaagggg gagacccagc gatcgtggaa catccagaac
    29761 gaattttgac tgcacgctac acaatggagc ttcctgctaa acaaagcgga gttgtgtcaa
    29821 aaatcgtggc aaatgaatta ggaattgctg caatgatgct aggtgctggg cgtaaaacca
    29881 aagaagaaga tattgatcac gctgttggat tgaaactgca taaaaaaatt ggtgacactg
    29941 tgacaaaagg tgagtcacta ttgacgatct atagtaacga cgaagaaatc acttctgtca
    30001 ttgaattatt atataaaaat attgaaattg gtgaatcggc aatgaagcca acgttgattc
    30061 atgacatcat cacagaataa aggaaaaaag cggactttac cgttttaaag aaaaggggaa
    30121 aagaaatgga actaaaccga atgatcgacc atactatttt gaaagcagac gcatctaaag
    30181 aagctgtgat gcaaattatc gaagaagcaa aaaaatacca tttttattct gtctgcatca
    30241 atccagcatg ggttgcgcta gcgaaagaac aattacaagg tgagccagta gccgtatgta
    30301 cagtaatcgg gtttcctcta ggagcaaaca cacccgagac aaaagcattt gaaacaacag
    30361 atgccatcaa taacggtgca gatgaagtcg atatggtcat caatatcggt gctttgaaat
    30421 caaaggatga tcaacaagtc caaaaagata tcgaagcagt tgttgaagca gcaaaagatc
    30481 gtgcgcttgt caaagttatt attgaaacat cgttattaga tagagaagaa atcatccgag
    30541 cttgcgaaat agcaaaagct gcaggcgctg attttgtcaa aacatcaact ggtttctcaa
    30601 caggcggagc aaaggtagag gatgtccgtt tgatgcgtga aactgtagga ccagaaatgg
    30661 gtgtcaaagc ttccggcggt atccataacg aagaagaagc aatggcaatg atcgaagctg
    30721 gtgctacaag aatcggaaca agcgcaggag tagcaatcgt atctggttca actggtgaag
    30781 gctattaatt ttttcaaagg agaagctcat ggcagaactc aaacaagaat ggatcaacac
    30841 agctattgaa gcgttagata aagcctatgt tccttattcc cattttccgg taggcgcttg
    30901 tctcgtaacg gaatcaggca agatttatca aggcatcaac attgagaatg catcatttgg
    30961 attaacgaat tgtgccgagc gcacagcttt tttcaaagca gtatctgaag gagaaaaaac
    31021 atttacgcat ctagtcgtag cggggcacac ccccgatcct atctctcctt gtggtgcatg
    31081 tcgacaagta atggcagaat tctgttcccc tgatatgccg gttacgctag taggagacaa
    31141 tggtgtgacg aaggcaacga ccgttcgtgc actgctgcct tatgcattta ctgaaaaaga
    31201 tctataactc atgttcattc agtcagctga ctgttgaaaa ataaaacaat tattttagga
    31261 ggctttacag aaatgaaaaa agcaaaatta tttggtttag gtgctgtcgc actagcagca
    31321 gggttatttt taggtgcatg cggcaacaac ggctctactg attcatccgg tggcaaaagc
    31381 tcatcagaca caacaactgc agcattgatc acagacacag gtggtgtaga cgaccgttca
    31441 ttcaaccagt cagcttggga aggactagaa aaatggggga aagatcaagg actttcacga
    31501 ggaaatgatg gcttccaata tttccaatct tcaaatgaat cagattacat cccaaatatt
    31561 gaccaagcat tgaatgctgg attcaaaacg atttttggta ttggttataa actaaaacca
    31621 gcgatccaag aacaagcaac aaacaataca ggtacgaact ttgttattat cgatgatgtc
    31681 atcgatggat tagataacgt tgtatctgca actttcaaag acaacgaagc ttcttatctt
    31741 gcaggtgttg cagcagctta tacgactgaa acaaacgtag tcggctttat cggcggtgta
    31801 aaaggtgaag taatcgatcg ttttgatgct ggattcaaag ccggtgttga tgcaggtgcg
    31861 aaagaattag gaaaagaaat caaagtattg aatcaatatg ctggtgactt ctctgctcca
    31921 gataaaggac gttcaattgc tcaaggtatg tatgcacaaa acgcagatat catcttccat
    31981 gcttcaggcg gtacaggtaa cggtgtattc caagaagcaa aatcattgaa cgaatcaggc
    32041 gacaaaaaag tttgggttat cggtgttgac cgcgaccaat cagacgaagg cgaatacaca
    32101 ttaaacggcg aaaagaaaaa cttcacattg acatctacac tgaaagcagt tggaacagta
    32161 gtagaagatt tggcacaaaa atcagcggac ggtaaattcc ctggcggcga acacactgtt
    32221 tatggattga aagaagacgg tgttggtcta acagaaggac aactttctga cgaagcgaaa
    32281 aaagcagttg atgaagcaaa agaaaaaatc atttcaggtg acgtaaaagt tccagaaact
    32341 cctgaagaaa actaaaacca tttctatagc cttgttagac acatgtttga caaggctatg
    32401 gtttgattag aacaagaatt gttcatttat ccaagctgcc ttgtgacagt ttggataagg
    32461 gaacaactcc ctgaagtaat gtttgtttca aaagaagaac gctcactaag cgtgaatctt
    32521 agtcaatcgt tgccctagat agaaatcgtt aggatgtgaa aaaaaggtgg ttcaagaaaa
    32581 tattgtcata gaaatgcaga atattaccaa agaatttgga acattcaagg caaatgacaa
    32641 catcaattta caagtaaagg caggagaaat tcatgcgttg cttggagaaa acggtgctgg
    32701 caaatctaca ttgatgaacg tgctttccgg attattagag ccgacatcag ggaaaatttt
    32761 gatgcgtggg aaagaagtaa agatcacaag tccgaccaaa gccaaccaat tagggattgg
    32821 gatggtccat cagcacttta tgcttgttga tgcctttact gtaacagaaa acatcgtgtt
    32881 gggaagcgag cctagtcgcg cagggatgct tgaccataaa aaagcacgaa aagagatcca
    32941 aaaagtttct gaacagtatg gattgtcagt caacccagat gcttacgttc gtgatatttc
    33001 agttggaatg gaacaacggg tagaaatttt aaaaacactt taccgaggag cagatgtact
    33061 gatttttgat gagccgacag ctgtattgac ccctcaagaa attgatgaat taatcgtgat
    33121 catgaaggaa ttagtcaaag aaggcaagtc gatcattttg attacgcata agttagatga
    33181 aatcaaagca gtagctgacc gctgtacagt tatccgccgt ggaaaaggaa tcggtacagt
    33241 caacgttaaa gacgtaacct cacaacaatt agccgatatg atggttggaa gaacggtttc
    33301 attcaaaacg atgaaaaaag aagcgaagcc tcaagaagtc gttttgtcta ttgaaaatct
    33361 agtggtgaaa gaaaatcgtg gattagaagc cgtgaaaaac ttgaacttag aggttcgtgc
    33421 tggcgaagta cttggtatcg ctggaatcga tggaaacggg cagtcggagt tgatccaagc
    33481 tttgactggt ttgcgaaagg cagaaagcgg acatatcaag ctaaaagggg aagacatcac
    33541 taataaaaaa ccccgaaaaa tcactgaaca tggtgtagga cacgtgccag aagaccgtca
    33601 taaatacggg ttagtcctag atatgacatt atctgaaaat atcgcactac aaacctatca
    33661 tcaaaaacct tacagtaaaa acggcatgct gaattattca gtaatgaatg aacatgccag
    33721 agaattgatc gaagaatatg atgttcgaac aacgaatgaa cttgttcccg caaaagcttt
    33781 atcaggcgga aatcagcaaa aagcaattat cgctcgtgaa gtcgaccgag atcctgatct
    33841 gttgattgtt gcaaatccaa ctcgtggact ggatgtaggt gcgatcgaat ttattcataa
    33901 acgtctgatc gaacaaaggg acaaatacaa agcagtgtta ctgatcagtt tcgaattaga
    33961 agaaatttta aatgtttcgg atcgtattgc tgttatccat gaaggagaaa ttgtcggtat
    34021 cgttgatccg aaagaaacat ctgaaaatga attaggtcta ttgatggcgg gctatacctt
    34081 agaagaagca cgaaaagagt tagaaaaggg ggctggcgca catgaatgat cgcagcgaaa
    34141 aattccgtaa tttattagtg cctgtacttt cagttgtttt aggattgatc ttaggcgcga
    34201 tattgatggc tgcttttggt tttcagccca tccaaggtta ttcttctatg ctagatgcag
    34261 cccttggttc gcaaagaggg ataggtgaaa cgttaaggca agcaacgccg ttgattttta
    34321 cagcattagg tttttctgta gctaactcag ctgggttttt caatattggg ctttctggac
    34381 aagcactttg cgggtggatc gtcagtgtct gggtggcact tgcgtttcca gatctgccaa
    34441 aagttgtttt gcttccgtta tgcgtgatct taggtgcctt agctggtgcg atggcagcgg
    34501 ctgttccagg tttacttcga gcattttttg gaacaagtga agtgatcgta acaatcatga
    34561 tgaactatat cttattatac gtaagcacat ttgctttaca tgaagtgatg cctgaaagct
    34621 atcgctcaag tttggactct tccaatatga tcagtcaaaa tgcctcactg cggttaccat
    34681 ggttaacaca agcttttggc ggctctcgcg taaactcagg attattttta gcaatcatcg
    34741 cgcttatttt cgtttggctg ctgatgaaga aaacgacttt aggatttgaa atccgttctg
    34801 ttggattgaa tccttttgct tctgagtatg caggaatgag tagtaaacgt acgatcgttt
    34861 tgtcgatggt catttctggc gctttggcag ggcttggcgg tgtggttgaa ggattaggaa
    34921 cgtatcaaaa cttctttgtt caaacgactt cgctatctat tggatttgac ggaatggctg
    34981 tttccttgct tggttctgga tcagcaatcg gtattctttt atcagcgatc ttgttcagta
    35041 ttttgaagat cggtggactt ggcatgcaga caggagcagg tgttccgttt gagatcgtca
    35101 atgtatcgat tgctttgatt atcttctttg tcgggatcaa cttcctgatt cgtttcgtca
    35161 tggctaaagt cttccaaggg aaaaaacaag aagagatcgt tgcgacaata gaatcgaaac
    35221 caacgaatca aaaccatgaa gggggcgaac tataatgtcg actatcgaat taattgcctt
    35281 gatccttact tctacactgg tttattcaac accgctaatt ctaacgtcac taggcggaac
    35341 tttttctgaa cgaagcggga tcgtaaacgt agggctagag ggaatcatgg tcatgggtgc
    35401 ttttagttcc atcgttttca acttgacgat gagtccatca cttggttcta tgacaccatg
    35461 gatcgctatt ttagtgggtg gcgtcgtagg tatcctattt tctctgattc acgcagtagc
    35521 aacagtcagt ttgcgtgctg accatattat cagcgggaca gtaattaatc tgatggcacc
    35581 tgctttaggt gttttcttga tcaaagcatg gtatggtaaa ggtcaaactg ataacatcac
    35641 gcagaatctg gggtattttt ctttcccagg attagcagat attccggtca ttggaccaat
    35701 tttctttaaa aatacattct tacctgctta ttgtgctatt ttgattgctg tcctagcttg
    35761 ggtgatcatc ttcaagacga aattcggctt gcgcctccgt tcagttggtg aaaatcctca
    35821 agctgccgac acattaggta tcaatgtata tgctatgcgt tattcaggcg tactgatttc
    35881 tggcttgtta ggcggtatgg gcggtgctgt ttttgctcag tcgatttccg gtaacttttc
    35941 agctggtacg atcgttggac aagggttcat ctccatggct gctatgatct ttggtaagtg
    36001 gaatccttta ggggcaatgg gtgcctcatt gttctttggt tttgctcaaa gcttgagtat
    36061 cattggcgca caattaccag ttatttcttc gattccgcca gtattgctgc aaattgctcc
    36121 atatttgctg acaatcattg tgctggttgt tttcttaggg aaagcttctg gtccaaaagc
    36181 aaacgggaag aactatatca aatcaaaata atcttttagc gaataaaaag ggtgggttac
    36241 gggaagtgtt tttcgtctcg ccctttttgt tttaaaggca ataaaaataa aaagaaagaa
    36301 ggaataatca tgtttaaacg cgtacatctg atcgtcatgg attcagtagg aattggagaa
    36361 gcaccagatg ccgataagtt cggtgatgtt ggttctgata cattaggaca cattgcgaaa
    36421 gaagcaggat tgaacgttcc acatttagag cagcttggtt taggaacgat ccgtcctctt
    36481 gaaggtgtgg aatcagtgaa agatcatcaa ggctatgcga cgaaattaga agaagtttct
    36541 gtagggaaag atacgatgac aggccactgg gaaatcatgg gattgaatat caaaactcca
    36601 tttcgtgtgt ttcctaatgg attccctgat gatctgatca aacaaatcga agaattttct
    36661 ggccgtaaag tcgtatgtaa caaaccttat agcggaacag ctgttattga cgattacggt
    36721 gaacaccaaa tgaaaacagg cgatttaatt gtatatactt ctgccgatcc tgtccttcag
    36781 atcgcggcac atgaagaaat cattccttta gaagaattat atcggatttg ccaattcgtt
    36841 cgtgatataa caaaagatga tccgtatatg attggccgaa tcattgcacg tccgtacgtg
    36901 ggtgaaccag gagacttcac acgaacaagc aatcgccatg attatgcgtt gaatccattc
    36961 ggacacacag tccttgattc tttgaaagaa gcaggaaagg acgtgattgc tgttggaaag
    37021 atcaacgata ttttcaacgg acaaggaatc actgaatctg tccgcacgaa aagcaatatg
    37081 gatggcgttg accagctgct gaaagttatg aaaaacgatt tttcaggaat cagctttacc
    37141 aatttagtag attttgatgc attatacggt catcgtcggg atgtagtcgg ctatgcgcat
    37201 gcgattgaag aatttgactt acgaattcca gaattgcttg aagcgatgga agaagacgac
    37261 ttgctaatga ttacagccga tcacggaaac gatccgacgt ttactggtac cgatcatacg
    37321 cgagaatatg taccgttatt ggtttacagt aaaaaaatga aagaacaagg ggcattgcct
    37381 caaggatatt attcagatat ttcagcaacg attgctgaga actttagtgt ttcttcgact
    37441 gaaaatggcg aaagcttctt gaaattatta aaataatctt tttaaatagg agaaagcaaa
    37501 tgaccaagtt aagcgagatg ttacaagaaa cgacccagtt tatccttgat aaaggagtaa
    37561 aagaaattga ttttggtttg attctaggtt caggattagg tgaattagca gatgaaatcg
    37621 aagatgctgt aatgatttct tatgatgaga tcccgttctt tcctgtttcg acagtagttg
    37681 ggcatgcagg aaagctagtt tatgggtctc tggcaggcaa gaaagtgtta gccatgcaag
    37741 ggagattcca ctactatgaa ggtcattcca tgcaaacagt gacctatcca gtacgtgtga
    37801 tggccggact aggggcccat tcagtcattg tgactaatgc atgtggtggt gtcaacgaat
    37861 catttgtacc aggagatttg atgctgatta ccgatcatat caattttact ggtgaaaatc
    37921 ctttgatcgg tccgaatgaa gatgcaatgg gccctagatt tccagatatg agtcaagctt
    37981 atacgcctga ctatcaagca acagcaaaag cagcagcaga aaaactaggt ttggatctta
    38041 aagaaggcgt atatatgggc tattctggac caacttatga aacgcctgcg gagatccgaa
    38101 tgtctcggac aataggagcc gatgcagtag ggatgtcgac tgttccagaa gtcatcgtag
    38161 cagctcacag cggattaaaa gtgttaggaa tctcttgtat taccaactta gcagcaggta
    38221 tgcaagcgaa tctgagccat gaagaagttg tagaaacaac tcagagagtc aaacaatctt
    38281 ttaaagcatt ggttaaagaa acattgacat tattgtaata ggaggaacaa taaaatgagt
    38341 gtgcatattg aagcgaaaga aggcgagatc gccgataaga ttttacttcc gggagatcct
    38401 ttgcgagcta aatatattgc ggagactttt ttggaagatc cagtttgtta caatcaggta
    38461 cgcggtatgt taggctttac cggcaaatat aaaggagaac gtatttctgt tcaaggaaca
    38521 ggaatgggaa tgccatcagc caccatctat gcccatgaat tgatccagtc gtatggtgta
    38581 aagaaattga ttcgagtagg tacatgcggt gcattgtcaa aagacgtcca tgttcgtgat
    38641 ttagtactag cacaaggtgc agcgaccagt tcttcgatga tcgaaaaaaa tttccaagcc
    38701 tttcattttc ctccaatcag tgatttcaat ctattactga aagcatatga gatagctaaa
    38761 gaaaagggct atacggttca tgtaggaaat gttctttcgg aagattcttt ctacaaagat
    38821 gatttgacag agacgttcca attagctgaa cttggcgttt taggagtgga aatggaagca
    38881 gcggctcttt attacttagg cgcgaaatac catgtgcaga cattaagttt gatgaccgtt
    38941 agcgaccatt tgatcaccgg tgaagaaaca acagctgcag aacgtcaatc tacgttcaat
    39001 gaaatgatcg aagtaggatt ggaaacagcg atagcagaat aagaaggaaa tgaaaaagct
    39061 gccctgcatt aagtaataag aacatccaag caaaaatagc tcctcatgtt tttgacttgg
    39121 ctggacttat tattgcaaat gtaagctttt gaacaccgtt taccaagagg ttgtgaatct
    39181 gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa
    39241 tattgtgact tgtgaccgag aagttggcag gagaaaaccg tttatacgaa aaaagggact
    39301 gtgacaaatc gtgtcacagt ccctttttta tagtgttttt tataggtagt attttttagc
    39361 aactgttaag tcatcattta attcataaac aagtggttga cctgttggaa tttcaagatc
    39421 catgatgtct tcgtcagaga taccttcgat atgtttagct agggcgcgta atgagttacc
    39481 atgtgcagct actaaaacag ttttgttgtc tagtaatgct ggagcaattt catcttgcca
    39541 gaatggcaaa gcgcgttcta atgtcacttt caagttttct ccaccaggaa tatcgcgttt
    39601 gtctaacatt gcgtaacgac ggtcatttgc tgcagaacct tcgtcagttt cttccattaa
    39661 tggaggaaga gtatcgtaag aacgacgcca gatgtgcact tgttcgtcgc cgtatttttc
    39721 agctgtttct tttttgttca aaccttgtaa tttaccatag tgacgttcgt ttaagcgcca
    39781 tgatttgatt tgtggtaccc aaagttgatc tgaatattct aagatcaagt tacatgtttt
    39841 gattgcacga gttaaaacag aagtgtaagc aacatcaaat tcgattccag cttctttgat
    39901 tttgcgtccg ccttcttttg cttcttcgat tccttctggt gctagattta catcagccca
    39961 accagtaaat tggttcaatg cattccattc actaagaccg tgacgagaaa aaactaattt
    40021 tggcatgttt agctctcctt ttaagatagg tttatatttc tgaaattttc agttctgttt
    40081 tattgtacaa ttttttggaa gagatttcca tcgaaaaatt agataagatt ctatgagaaa
    40141 acaagtagag aatataaaaa aacgaacagt gcccacgcaa ctgttcgttt ttaggtatta
    40201 gatgcataat tgctcgattt ctttttcagg taaattgata tatttataac atgttccaac
    40261 tgaaacaccg cattttgaag aaataaattg gatcgtttct tttttttcat aatagagacg
    40321 tcggattttt ttgatcgttt ttgcatcgat ttttggtctt ccgccgattt ttcctttttt
    40381 acgagcttca tctaatccaa ctaatgtacg ttctttgatc aatgcgcact ccatgtttgc
    40441 taggttttcc attaattgga aatagatttt tcccatttcg cgcgtatcga ttccttcatc
    40501 aagactaatc aagtgcagcc cgcgatcttt gaataattgc gtgagttctg ttaattgccg
    40561 cgtagatttg cctaatcgat gaagttggca aatgactagt gcatcgcctt gttgcatgtc
    40621 agcgatcact gactccaaat gaacagcagt agaatcattt tgttccactt catatgtctc
    40681 atggaaaatt tcttgacaac caaagtcaga taattgatca acctgagttg ttaaatcaac
    40741 gtcatttatg gttgtgcgtg cataaccaat aattttcatg gtgattcctt tctagcagtg
    40801 attttctaac tggatataac agtaaaaatg tagcataaag tacgtcaaaa agatagtttt
    40861 ttcgcaaatt ttccgctgtc tttttttatt ggatgatcaa gcagtttttc tttatttacg
    40921 acggattgcg tcaagaatct caacgccttt ttctgagccg acaactactt tagaaaccat
    40981 gtttaggaaa agcccatgtt ctactacccc aacaagttca tctaaatagg tagctaagct
    41041 ttctggctgt tcgatgactt ctagatgcag atcaatgatg taatgaccac catctgtggt
    41101 taagacttct ccgttttcgt ctgtacgcaa gacgggttgg aatctttttt cttcaaaaat
    41161 atgaagtagc tgttgggagc cataagggat cacttcaact gggagtggga acttcccaag
    41221 tttatcgact aatttagaag aatcaacgat ccaaatcgtt tcttttgagt acgtagcgac
    41281 tattttctcg aaaagcaaag cagcgccgcc gcctttgatc ccttgaaaat cagcactgat
    41341 ttcatcagca ccgtctattg tcaaatcgac tacggggact tcatcaatac tctttaacgg
    41401 tattcctaat ttttccgctt gttcttttgt cgcatttgaa gtagtaactc ctgtgatacg
    41461 taaaccttct tcacgcatac ggcgaccaat ctcttctacc atataatagg cggttgaacc
    41521 ggtcccgaga cctacgatca tcccgtcctt tacgaatttc gcggcttcaa tgccagctaa
    41581 ttgttttaaa ttcatctttg cgtccacctt tttttagttt gagcatcgtg ctttcctcac
    41641 tattttagcg tgatttacgg taaaaagata ccattctttt ttattccaat aaagcttttt
    41701 caaaaaaaat tgcatctctt gttgacatgt tttttcattc gtgatattct aacaaaggtg
    41761 ctttatctac aggtttctgt gaagaaacgt gctgactgtt tatcaaagca tatttgataa
    41821 ggatgcagac attgcattta ttttttatca caaaaaaaga accacctgga tgtgtggaac
    41881 tagacgcaaa tcgtgggaag gaggaaacaa ggaatgccta caattaacca attagtacgt
    41941 aaacctcgta aatcaaaggt ggaaaaatct aattcacctg ctttgaacaa aggatataac
    42001 agctttaaga aaactcaaac taacgtaaac tcacctcaaa aacgtggggt agcaactcgt
    42061 gtgggtacaa tgacacctaa aaaaccgaac tcagctttac gtaaatatgc acgtgttcgt
    42121 ttgtcaaact taattgaagt tactgcttat atcccaggga ttggtcataa cttacaagaa
    42181 cacagtgttg tattattacg cggtggtcgt gtaaaagact taccaggggt acgttaccat
    42241 atcgttcgcg gtgctttaga tactgctggt gttaacgacc gtaaacaaag ccgttctaaa
    42301 tacggaacta aaaaacctaa agcttaattt taattattaa acaaatgaaa cttgcttcta
    42361 ctatatagaa gtaattacta tgaacaatat ttcggaagga ggagttacgg atgccacgta
    42421 aaggtcctgt tgcaaaacgt gatgttttac cagatcctat ttataactca aaattagtaa
    42481 ctcgcttgat caaccgtgta atggttgacg gaaaacgcgg gattgcagct aacattatct
    42541 ataatgcctt tgacttgatc aaagaatcta caggtaacga tccattagaa gtgtttgaac
    42601 aagcaatgaa aaacgttcaa cctgttcttg aagttaaagc acgtcgtgtt gggggttcaa
    42661 actaccaagt accagttgaa gttcgccctg aacgccgcgt tactctaggt cttcgttggg
    42721 tagtaaacta cgctcgcttg cgcggtgaac acacaatgga agaacgctta gcaaaagaaa
    42781 tcatggatgc tgcaaacaac acaggtgctt ctgttaaaaa acgtgaagac actcataaaa
    42841 tggctgatgc caaccgtgcg tttgctcact atcgctggta agattctctc tgtttggtcg
    42901 gcttttataa gtcgacctca gatagtgtcc ttatttttag caactagata gtaaagagag
    42961 gagaaacaag gagaatgacc agagaatttt ctttacaaaa cactcgtaat atcggtatca
    43021 tggcccacat cgatgctggt aaaacgactg ctactgaacg tatcttgtac tatactggta
    43081 aaatccataa aattggtgaa actcatgaag gagcttcaca aatggactgg atggaacaag
    43141 aacaagaacg tggtattacg atcacttctg ctgctactac agcacaatgg aagggtcacc
    43201 gaatcaacat catcgacaca ccaggacacg tagacttcac agttgaagtt gaacgttcat
    43261 tgcgtgtact tgatggtgct gtaactgttc ttgatgcaca atcaggtgtt gaacctcaaa
    43321 ctgaaacagt ttggcgtcaa gcaacaactt atggtgtacc tcgtatcgta tttgcaaaca
    43381 aaatggacaa gatcggtgct gacttcttat attcagtatc aactttacat gaccgtttgc
    43441 aagcaaacgc tcacccaatc caattaccaa ttggtgcgga agacgacttc acaggaatta
    43501 tcgaccttgt aacaatgaag gctgaaatgt atacaaacga tcttggaaca gagatcgaag
    43561 aaacagaaat tcctgaagaa taccgcgaat tagctgaaga atggcgcgaa aaattggttg
    43621 aagctgtagc tgaaactgat gaagaactta ctttgaaata cttagaaggt gaagaaatca
    43681 ctgaagctga gttaaaagaa ggtatccgcc gcgcaacagt taacgtagaa ttctacccag
    43741 tactatgtgg ttcagcattt aagaacaaag gggttcaatt actattggac gctgttcttg
    43801 attacttgcc atcaccgtta gatatcccag ctatcaaagg gattgaccct aaaactgatg
    43861 aagaagtaga acgtcctgca gacgacagcg ctcctttctc agctttagca tttaaagtta
    43921 tgactgaccc attcgtaggt cgtttaactt tcttccgtgt gtactctggt gttttacaat
    43981 caggttcata cgtacaaaac gcaacaaaag gcaaacgtga acgtgttggt cgtatcctac
    44041 agatgcatgc caactctcgt tcagaaatct ctgaagttta cgctggagat atcgctgctg
    44101 ctgttggttt gaaagacaca actactgggg acacactatg tgatgaaaag agtctagtta
    44161 ttcttgaatc catggaattc ccagaaccag ttatccaagt cgctattgaa cctaaatcaa
    44221 aagctgacca agataaaatg ggtgtggctt tacaaaaact atctgaagaa gatcctactt
    44281 tccgtgctga aacaaacgtg gaaacaggcg aaacaatcat cgctggtatg ggtgaacttc
    44341 acttagatat catcgttgac cgtatgcgtc gtgaattcaa agttgaagct aacgtaggtg
    44401 cgcctcaagt atcttaccgt gaaactttcc gtgctggcac tcaagccgaa ggtaagtttg
    44461 tacgtcagtc tggtggtaaa ggtcaatacg gtcacgtatg gattgaattt acaccaaatg
    44521 aagaaggtgc tggtttcgaa tttgaaaatg cgatcgtcgg tggggttgtt cctcgtgaat
    44581 acattccagc tgttgaaact ggtttgaaag atgctatgga aaatggtgtc ttagctggct
    44641 atccattagt agatatcaaa gctaaacttt acgatggttc ataccatgat gtcgattcaa
    44701 atgaaactgc cttccgtgtt gctgcttcta tggcattacg tgcagcagct aagaaagcta
    44761 atccagtaat ccttgaacca atcatggctg tagaagttgt tatccctgaa gattatttag
    44821 gtgatgttat gggtcacgta actgctcgtc gtggacgtgt tgaaggtatg gaagctcgcg
    44881 ctggcggcca acaagttgtt cgtgcgatgg ttccacttgc tgaaatgttt ggttatgcta
    44941 caactcttcg ttctgctact caaggtcgtg gtacatttac aatgacattt gaccactacg
    45001 aagatgtacc aaaatcagta caagaagaaa tcattaagaa aaacggcgga aaagctgaat
    45061 aatttcttaa aaccggatag tcctgcaaag agattatcca aagagactat atttttaata
    45121 tagtttcgtg taaaataatt atcgatgata tgggcatgtg gtgtaccttg tgtacatcat
    45181 atacctatca gacaaataaa aaatatattg tatttttagg aggacatttt aaaatggcaa
    45241 aagaaaaatt tgaccgttct aaaccacacg ttaacattgg tactatcgga cacgttgacc
    45301 atggtaaaac tacattaaca gctgcaatca caactgtact atctaagaaa aacggcggcc
    45361 aagctatggc ttacgatcaa atcgatggtg ctccagaaga acgcgaacgc ggaatcacaa
    45421 tctcaacagc tcacgttgag tatgaaacag atacacgcca ctacgctcac gttgactgcc
    45481 caggacacgc ggattacgtt aaaaacatga tcactggtgc tgctcaaatg gacggagcta
    45541 tcttggtagt ttctgctgct gacggcccaa tgcctcaaac tcgtgaacac atcctattgt
    45601 ctcgtcaagt tggtgttcct tacatcgttg tattcttgaa caaagtagac atggttgatg
    45661 acgaagaatt actagaatta gttgaaatgg aagttcgtga cctattaaca gaatacgaat
    45721 tccctggtga cgatgttcct gtagttgctg gatcagcttt gaaagctcta gaaggcgacg
    45781 cttcatacga agaaaaaatt cttgaattaa tggctgcagt tgacgaatac atcccaactc
    45841 cagaacgtga caacgacaaa ccattcatga tgccagttga agacgtgttc tcaattactg
    45901 gacgtggtac tgttgctaca ggtcgtgttg aacgtggaca agttcgcgtt ggtgacgaag
    45961 ttgaagttgt tggtattgct gaagaaactt caaaaacaac agttactggt gttgaaatgt
    46021 tccgtaaatt gttagactac gctgaagctg gagacaacat tggtgcttta ctacgtggtg
    46081 ttgcacgtga agacatccaa cgtggacaag ttttagctaa accaggtaca atcacacctc
    46141 atacaaaatt ctctgcagaa gtatacgtgt tgacaaaaga agaaggtgga cgtcatactc
    46201 cattcttcac taactaccgt ccacaattct acttccgtac aactgacgta acaggtgttg
    46261 ttgaattacc agaaggaact gaaatggtta tgcctggcga caacgtaaca atggaagttg
    46321 aattaatcca cccaatcgct atcgaaaacg gtactaaatt ctcaatccgt gaaggcggac
    46381 gtacagttgg tgccggtgtc gttacagaaa tcaaagctta attcacttaa gttttaaagt
    46441 aaagcaacat ttattcggac gtaatcgctc ggaacgaagg tcttaggatc tttgttccga
    46501 gcttttttat ttattgcagg atcaggaaaa aagacaggag tattataaat agaagaaaca
    46561 cgaaataaaa gagtcagtat tttctaaatg aaactttatg aaaacaaaat gttttttaca
    46621 agaaaaagcc ttgctttgtg agtttatata tattataatg acaaaggtgc gtttacttta
    46681 gtgaattgca cagacgtaat cagaatgatt tcgtcggcta agaaacgaga ggttgcgaca
    46741 cgcccggacg ctttgccacg aacgagcgtg acggaaattt tcgtggagct atgtctactt
    46801 ttcaaaatag gcgaaggagg gaacaagatg gcaaaacaaa aaattcgtat ccgtttaaaa
    46861 gcgtatgaac accgtatttt agatcaatca gcggataaaa tcgtggaaac tgcaaaaaga
    46921 actggagcta gcgtgtctgg tccaattcca ttaccaactg aacgcagtct ttacacaatc
    46981 atccgtgcga ctcataaata caaagattca cgcgaacaat tcgaaatgcg tacacacaaa
    47041 cgtcttatcg acattgtgaa cccaacacca aaaactgttg atgctttgat gaagctagac
    47101 ttaccatctg gtgtaaacat cgaaatcaaa ttataaaact aaaacaaaaa taatggaggt
    47161 gtactcatga ccaaaggaat cttagggaaa aaagtgggaa tgacacaaat cttcactgaa
    47221 tctggtgaat taattccagt aactgttgta gaagctactc caaacgtagt actacaagtg
    47281 aaaacaatgg aaacggatgg ctacgaagct attcaagttg gttaccaaga tatgcgtgaa
    47341 gttttatcaa acaaacctgc gaaaggtcat gttgcaaaag caaacacggc tcctaagcgc
    47401 ttcattcgtg aattcaaaaa tgttgagcta ggagaatatg aagtaggaaa agaaattaca
    47461 gtagatgttt tccaagcagg agacattatt gatgtaacag gtactacgaa aggtaaagga
    47521 ttccaagggg ttatcaaacg ccacggacaa agccgcggac caatggctca cggttctcgt
    47581 taccatcgtc gtcctgggtc aatgggtcca gttgcaccta accgtgtatt taaaaacaaa
    47641 cgtcttgctg gccgtatggg tggcaaccgc gtaactatcc aaaacttgga agttgtacgt
    47701 gtcgatgcag aaagaaatgt tatcttgatc aaaggtaatg ttcctggagc taaaaaatct
    47761 ttaattacta tcaaatcagc tgtgaaagct aaataattgt aggaagaaag gaggaactaa
    47821 ggaatgccga atgtagcatt attcaaacaa gatggaagcc aaaacggtga aatcacactt
    47881 aacgaagaaa tcttcggaat tgaacctaat gaaagtgttg tttacgatgc aatcgtaatg
    47941 caacgtgcgt cattaagaca aggaacacac gcggttaaaa accgtagcgc tgttcgcggc
    48001 ggcggacgta aaccatggcg tcaaaaagga acaggtcgtg cacgtcaagg atctatccgc
    48061 tcaccacaat ggcgtggagg tggcgtagtc tttggaccaa caccacgttc ttacagctac
    48121 aaacttccta aaaaagttcg tcgtttagca atgaaatctg tattgtcaga aaaagttgct
    48181 gaaaacaact tggtagcaat cgaaggcttg aacttcgacg caccaaaaac aaaagaattc
    48241 aaacaagttc ttgctaactt atctatcgac tctaaagtat tggttgttct agaaaaaggt
    48301 aacgactttg ctgctttatc agcacgtaac ttaccaaacg tttctgtagt aacttctgat
    48361 aatgtgagtg tgctagacgt tgtatcaaat acaaaagtgt tagcaacaca aactgctctt
    48421 actcaaattg aggaggtgct tgcataatga acttactaga cgtaatcaaa cgcccagtga
    48481 tcactgaaaa atcaatgctt gccatggatg acaagaaata cacttttgaa gtggacactc
    48541 gcgcaaacaa aactttagta aaacaagcgg tagaagctgc ttttgacgtt aaagtcaaaa
    48601 acgtgaacat cgtgaacgta cgtgcgaaat tcaaacgtat gggcaaatat gcaggatata
    48661 caaaaaaacg tcgcaaagcg atcgttacat tgactgaaga ttcaaaagaa atccaattat
    48721 tcgaagctgc tgaataagca ctaatagata actcattaaa ttaggaggga aaaacgtggc
    48781 gattaaaaag tacaaaccta cctcaaatgg tcgtcgtaac atgacaggtt ctgattttgc
    48841 tgaaatcaca acatcaacac ctgaaaaatc attgttacaa ccattgaaaa accatgctgg
    48901 acgtaacaac aacggccgca tcactgtacg tcatcaaggt ggcggacaca aacgccaata
    48961 tcgcgtgatc gacttcaaac gtaacaaaga taacgttgtt gcaactgttc aaacgatcga
    49021 atatgatcca aaccgttcag ctaacatcgc gttagtacac tatgaagatg gagtgaaagc
    49081 ttacatctta gcacctaaag gactagaagt gggtatgaaa cttgtttcag gtccagaagc
    49141 agatatcaaa gtcggaaatg ctcttccatt agaaaacatt ccagttggta ctgttatcca
    49201 caacattgaa atgaaacctg gtaaaggtgg acaattgatc cgttctgctg gtacaagtgc
    49261 tcaagtactt ggtaaagaag gcaaatacgt attgatccgc ttgaactctg gtgaagttcg
    49321 tatgatctta gctgcatgcc gtgctacaat cggttctgta ggtaacgaac aacacgaatt
    49381 aatcaacatc ggtaaagctg gacgctctcg ttggatgcgt aaacgcccaa ctgtacgtgg
    49441 tagcgtaatg aaccctaatg atcacccaca cggtggtggt gaaggtaaag ctcctatcgg
    49501 acgtaaagca ccagtttctc cttggggtca accagctctt ggttacaaaa ctcgtaacaa
    49561 aaaagctaaa tcagacaaac ttattgttcg tcgtcgcaaa aataaataat tactttgacc
    49621 atgtgtcgca cattttgaga ggaggttcac catgggtcgt agtttaaaga aaggaccttt
    49681 tgtcgatgag catttaatga aaaaagttga agctcaagca ggggttgaaa agaaaaaagt
    49741 tatcaaaact tggtcacgcc gttcaacagt tttcccaagt tttgttggat ataccatcgc
    49801 agtttatgat ggacgtaaac atgtaccagt ttacatccaa gaagacatgg taggacacaa
    49861 attaggtgaa tttgcaccta caagaactta tcgtggacat gctgctgacg ataagaaaac
    49921 gaaacgctaa tttgagggga ggataaatcc aaatggcaga acaaatcaca tcagctaaag
    49981 caactgcaaa aacagttcgc acttcacctc gaaaagcccg tttagtaatt gaccttatca
    50041 gaggtaaaag tgtcgcagat gcaatttcaa tcttgaaatt cacaccaaac aaatctgctg
    50101 gaatcattga aaaagtattg atgtcagcag ttgcgaatgc agaaaataac tttgacttag
    50161 atgttgaaaa cttggtagta agcgaagctt ttgttaacga aggacctaca atgaaacgtt
    50221 tccgtcctcg tgcaaaagga tcagcttctc caatcaacaa acgtacaagt catatcacgg
    50281 tagtcgtatc agaaaaataa ggagggataa tcagtgggtc aaaaagtaca tccaattgga
    50341 atgcgtgtag gcatcatccg cgactgggat gctaaatggt atgctgaaaa agagtatgct
    50401 gagttcttac acgaagattt aagaatccgt aaatttattt caactaaact tgctgatgct
    50461 gctgtatcaa cagttgaaat cgaacgcgca acaggtcgcg taaacatttc aattcacaca
    50521 gctaaaccag gtatggttat tggtaaaggc ggttctgaag tcgaaagcct aagaaaagaa
    50581 ttaaacaaat taactggtaa aagaatccat atcaatatcg tggaaatcaa aaaaccagat
    50641 ctagatgcaa aattagtagg tgaaggaatc gcacgtcaat tagaaaaccg tgttgctttc
    50701 cgtcgcgctc aaaaacaagc catccaacgc tcaatgcgtg ctggcgctca agggatcaaa
    50761 actcaagtat ctggtcgtct aaacggagca gatatcgctc gttcagaagg atactcagaa
    50821 ggaactgttc ctcttcatac tttacgtgcg gacattgatt acgcatggga agaagcagat
    50881 acaacatacg gaaaactagg agttaaagtg tggatctatc gtggagaaat tcttccaact
    50941 aaaaaaaaca ctgagaaagg agggaaataa tcatgttagt acctaaacgt gtaaaacacc
    51001 gtcgtgaatt ccgcggaaaa atgcgcgggg aagctaaagg cggaaaagaa gtagcattcg
    51061 gtgaatacgg tttgcaagct gttgattcac attggatcac aaaccgccaa atcgaagctg
    51121 ctcgtatcgc aatgactcgt tacatgaaac gtggtgggaa agtatggatt aaaattttcc
    51181 ctcacaaatc ttatactgcc aaagcaattg gggtacgtat gggttctggt aaaggggcac
    51241 ctgaaggatg ggttgcacca gtaaaacgtg gtaaaatcat gtttgaaatc gcaggcgttc
    51301 ctgaagaagt agctcgtgaa gcgttacgtc tagcttctca caaattacca atgaaaacta
    51361 agatcgtaaa acgtgaggaa atgggtggtg aatcgaatga aggttaaaga aatcagagaa
    51421 ttaaccactg ccgaaatgct tgatcaagaa aaacaattaa aagaagaatt gtttaatctt
    51481 agattccaat tagcaacagg tcaattagaa aacactgcac gtattaaaga agtacgtaaa
    51541 tcgattgcac gcatcaaaac tgtgttgcgt gaacaagcta agtaattatg gaaggaggcc
    51601 attaagcgta tgactgaagg aagaaatcaa cgcaaagttt atcaaggtcg cgtggtttca
    51661 gacaaaatgg ataaaacaat taccgttgct gtcgaaacaa agaaaaacca ccctatctat
    51721 ggcaaacgca tgaactattc taagaaatac aaagtgcatg atgaaaacaa cacagcaaaa
    51781 gttggcgaca tcgtgagaat tatggaaact cgtccattat cagctacaaa acgtttccgt
    51841 ttgttggaag tagtcgaaga agcagttatt atctaataac acgagatttt cctatataga
    51901 ttgaatgcaa aatctgaaag gaggatacac aacgtgatcc aagcagaaag tcgtttaaaa
    51961 gtcgctgata actcaggcgc acgtgaaatt ttgacgatca aagtccttgg tggatctggt
    52021 cgtaaaactg ctaacatcgg tgatgtgatc gttgctacgg ttaaacaagc aacgccaggt
    52081 ggggttgtca aaaaaggtga cgtcgttaaa gccgttatcg ttcgaacaaa atcaggagct
    52141 cgtcgtacag acggttcata tattaaattt gatgaaaatg ctgcagtgat tattcgtgat
    52201 gataaaagcc ctcgtggaac acgtatcttt gggccagttg cacgtgaact acgtgaaaac
    52261 aacttcatga agatcgtttc actagcacca gaagttttat aatcttgaga cacttatcaa
    52321 aggaggtgcg aaacagttat gtttgttaaa aaaggcgata aagttaaagt tatcactgga
    52381 aaagacaaaa acaaagaagg cgttgtacta gcagcgttcc cgaaaaaaga taaagttatt
    52441 gtcgaaggtg taaatgtcgt taaaaaacac caaaaacctt ctcaagctgc tcctcaaggt
    52501 ggaattgttg agatggaagc gccaattcat gtttctaacg tgatggtggt tgactctact
    52561 ggtgtagctg gccgagttgg ctacaaagaa gtagacggca aaaaagtccg tgtttctaaa
    52621 aaaaccggtg aagttttaga taaataaaag tttaaggaag gagggtctta ctgaatgaac
    52681 cgcctaaaag aaaaatatct taaagaagta actccatcat tgatggaaaa atttaactat
    52741 agctctgtta tgcaaacacc taaagttgaa aaaatcgtca tcaacatggg tgttggtgat
    52801 gcggtatcaa acgctaaaaa cttagataaa gcagttgaag aattaacttt gatcactggt
    52861 caaaaaccaa tgattacaaa agctaaaaaa tcaatcgctg gattccgttt acgtgaagga
    52921 atgccaattg gtgcaaaagt tacattgcgc ggagaaagaa tgtacgaatt cttagacaaa
    52981 ttggtttctg tttcactacc acgtgtacgt gacttccatg gtgtaagtaa aaaagccttt
    53041 gacggtcgtg gaaactatac tttaggtatt aaagaacaat tgatcttccc agaagtagat
    53101 tatgatttag tagataaagt acgtggtatg gacatcgtta ttgtaacaac agcaaactct
    53161 gatgaagaat ctcgtgagtt gttgacacaa ttaggcatgc cattccaaaa ataattaaag
    53221 gaggcgaact acgtggctaa aaaatcaatg attgctaaaa acaaacgtcc tgcaaaattt
    53281 tcaacacaag cttatactcg ttgcgaacgt tgcggacgtc cacattcagt ttatcgtaag
    53341 ttccatcttt gccgtatttg cttccgcgaa cttgcctata aaggtcaaat tcccggcgtg
    53401 aagaaagcta gctggtaaaa agtaaattcg cataagaagg aggtaaaaga tcaatggtca
    53461 tgacagatcc aattgcagat tttctaactc gtatccgtaa cgcaaacatg gttaaacatg
    53521 agtctttaga agtacctgca tcaaaaatta aacgtgacat cgctgaaatc ttgaaacgtg
    53581 aaggtttcgt tcgtgatgtt gaatacatcg aagatgacaa acaaggcgtg atccgtgttt
    53641 tcctaaaatt tggtaaaaac ggcgaacgtg ttatcacaaa cttaaaacgt atctctaaac
    53701 caggtttgcg tgcttatgtc aaagctgacg aagtaccaaa agtattgaac ggtttaggta
    53761 tcgcaatcat ttcgacttct gaaggtgttg tcactgataa agaagctcgt gaaaaaaata
    53821 tcggcggcga agttatcgct tacgtatggt aatcaagtag aatctacaag gaggtgtctt
    53881 taagtgagcc gtatcggaaa taaagtcgtt gttatccctg aaggagtaac agttactcaa
    53941 gacggaaaca acgttacagt taaaggacca aaaggtgaat tgactcgtac tttttcagca
    54001 gatatcaaaa tgaatatcga aggaaacgaa gtaactttca ctcgtccaaa cgacagtaaa
    54061 gaaatgaaaa caattcatgg aacaactcgt gcgaacttca acaacatggt tgtaggtgtc
    54121 agcgaaggtt tccaaaaagc gttagaacta atcggggttg ggtaccgtgc acaattacaa
    54181 ggatcaaaac ttgtattgaa cgttggttac tcacatccag tagaaatcac accaccagct
    54241 ggagtaactg ttgaagtacc agctaacaca caagtaattg ttaaaggtgc gaacaaagaa
    54301 gaagttggcg aattagctgc aaacattcgt ggaactcgtc ctccagaacc ttataaaggt
    54361 aaaggtattc gttatgttgg cgaatatgta cgccgtaaag aaggtaaaac tggtaaataa
    54421 tcgtttagaa aaattgcata gaagccagca gcaggaaaaa ggcatttggc tgaagtttac
    54481 cttcaaaaaa atacattttt acttgtgccg cagcttctat ttttcgaaac gtatttactg
    54541 cttgaaatag tagtacaata aaaataaaga ggtgacaatt gtgattacaa aaccagataa
    54601 gaacaaaaca cgtcaaaaga gacatcgtcg tgtacgtaac aatatctctg gtactgctga
    54661 gcgcccacgc ttgaacgttt ttcgttctaa caaaaacatc tacgctcaaa ttattgatga
    54721 cgtagcgggt gtaacgctag caagtgcctc taccttggat aaagaaattt caggtggaac
    54781 aaaaacagaa gcagcacaag ctgtcggtaa actagttgct gaacgtgcag ctgaaaaagg
    54841 cattaaagta gtagtctttg accgtggtgg atacctttac catggccgtg tgcaagcttt
    54901 agctgaagct gctcgcgaaa atggactaga attttagaag aaggaggaat accattcatg
    54961 gtttatatcg atccaaaaca tttggaatta gaagaccgcg ttgttgcaat taaccgtgta
    55021 acaaaagttg ttaaaggtgg acgtcgtcta cgttttgctg ctttagttgt tgtcggtgac
    55081 aaaaacggac acgtagggtt tggtacaggt aaagctcaag aagttcctga agcaatccgt
    55141 aaagcaattg aagatgcgaa gaaaaactta gttgaagtac ctatggttgg ttcaacaatc
    55201 ccacacgaag taatcggtgc ttttagtggt ggtagaatcc taatgaaacc tgctgtagaa
    55261 ggttctgggg ttgctgctgg tggaccagtt cgtgccgtat tagaattagc tggggtagca
    55321 gatatcactt ctaaatcttt aggctcaaat acaccaatca acgttgttcg cgcaacagtt
    55381 gaaggtttga aacaattgaa acgtgcagaa gaagttgcag aacttcgcgg caaatcagtt
    55441 gaagaattaa tcggataagg aggacaacaa taatggctga attaaaagtt actttaaaac
    55501 gcagtattat cggacgtcct caaaaccaac gcgatacagt taaagcgtta ggtcttacaa
    55561 agattaacag ttcagtggtt aagccttcta atgaagcaat taaaggcatg atcaatactg
    55621 tttctcacct agtggacgtt gaagaaattt aatttagaaa aatcataact agaacattaa
    55681 ggaggtgcca aacctatgaa acttcatgaa ttaaaacctg ctgaaggttc acgccaagta
    55741 cgtaaccgtg ttggtcgtgg tacttcatct ggtaacggaa aaactgccgg acgcggacaa
    55801 aaaggacaaa aagctcgttc aggcggcggt gtgcgtttag gatttgaagg gggacaaaca
    55861 ccattgttcc gtcgtttacc aaaacgagga ttcacaaacg tgaaccgtaa agactatgca
    55921 gtcattaatc ttgatgtatt gaaccgcttt gaagacggag ctgaagtaac tcctgttgct
    55981 ttagttgaag ctggaatcgt gaaaaacgaa aaagctggaa tcaaagtatt agctaatggg
    56041 gaactaaaca agaaattaac tgtgaaagca gcaaaattct ctaaagcagc tcaagaagca
    56101 attgaagcgg ctggtggatc aatcgaggtg atctaatgtt tagactatta aaagatgctt
    56161 ttaaagtaaa agacattaga tcgaaaatcc tatttactgt atttgtgttg tttgtatttc
    56221 gcctaggtgc acatattact gtaccaggcg taaatgctgc ttcattgcaa aacctttctt
    56281 ctttgccatt tttcaacatg ttgaacttgg taagtggtag tgcgatgcaa aacttttcca
    56341 ttttctcaat gggggtttca ccatacatta ctgcttctat cgtcattcag cttcttcaaa
    56401 tggacatcgt gcctcgattt gttgaatggt cgaaacaagg ggaagttggt cgtaaaaaac
    56461 tgaatcaagc gactcgatat ctgacattag tattggcatt tatccaatct gtcggtatca
    56521 ctgctggatt caatcaatac gctcaattag gtttcgtgaa taatcctaac gtagcaactt
    56581 acgtaacgat cggtttgatt ctaacagccg gtacgatgtt tgttacttgg ctaggagaac
    56641 agatcacaga aaaaggtatc ggaaatggcg tatcaatgat catttttgca ggtatcattt
    56701 ctagattgcc tgaaagtatc agagaaattt atgaagatta ctttataaat attgatcctt
    56761 cagacatatg gaaatctgct atttttgtgg ccattttact agtagcggtc ttgttgatcg
    56821 tgacattcgt aacatttttc caacaagctg aacgcaagat cccgatccaa tatacgaaac
    56881 gcgtagctgg cgcgcctaca agcagctatc tgccgttgaa agtaaatgca gctggagtta
    56941 ttccagttat ctttgctagt tcgtttattg ctacgccaaa tgctgtttta caagcattga
    57001 gcggtagatt cggttctgaa aactggtaca aagtgatgat ggagatcttt aactataata
    57061 cagcacctgg tgctacgatt tatacgattc tgatcgttgc cttcactttc ttctacgcct
    57121 ttgttcaagt aaaccctgag aaattagcgg aaaacttgca aaagcaagga agttatatac
    57181 caagcgtacg ccctggtaag gggactgaag agtatgtctc aagattactg atgcgtttaa
    57241 gtacagttgg tgcagtcttc cttggtttag tggcattgct tccaatcatt gctcaaatgg
    57301 tttgggatct tccacaatca atcggattag gcggtacaag cttactgatc gttatcggtg
    57361 tagctttaga aactgcgaaa cagttagaag gcttaatgct gaaacgtaaa tatgttggat
    57421 tcattgagta agaaagcgtg ttgaggtttc ccctcaacac acgttctcac acatttatta
    57481 ggagggaaaa caatgaacct cattttaatg gggctgccag gcgcaggtaa aggaacacaa
    57541 gctgaaaaaa tcattgatgc ttatggtatt ccgcacatct caacaggaga tatgttccgc
    57601 gcagcgatgc aaaacgaaac agctcttggc ctggaagcaa agtcttatat ggataaaggt
    57661 gcattagttc ctgatgaagt aacaaatgga atcgtgaaag agcgactagc tgaaccagat
    57721 acagagaaag gcttcttatt agatggcttt ccacgtacgc tagatcaagc aaaagctctg
    57781 gacgcaatgc taaaagattt gaataaaaaa attgatgctg tcatcgatat ccatgttggt
    57841 gaagaaatct tggtcgaacg tctagccggc cgatttatct gcagcaattg tggagcaact
    57901 taccataaaa ttttcaaccc tacaaaagtt gaagacacat gtgatcgttg cggtgggcat
    57961 gaattctatc aaagagaaga tgataaacct gagacggtta aaaatcgtct ggctatcaac
    58021 atcaaaaata gtgaaccaat cttggcttat tataaagaac aaggtttgct aaacactatt
    58081 gatggtgctc gtgaaatcga tgctgtattt gcagatgtac aaaaaatcat tgaaaaatag
    58141 cacgattgcc cgtagccgac ttgcattatg cagttactct tgtcaagttc aataaattat
    58201 gatagaatat gtcagtccga cttctagaga atcctctaga tgatccattt tgagaatttt
    58261 gaggtgggaa ctgcagtttc cgccatttta tcgtgtgaaa atgcgaaaca agtacaagga
    58321 ggtactgtgc gtggcaaaag aagatatgat tgaagtcgaa ggtacagtcg tcgaaacttt
    58381 gccgaatgca atgtttaaag ttgaactaga aaacggacac caagttcttg ctactgtttc
    58441 aggtaaaatt cgtatgcatt acatccgtat cttacctggt gacaaagtaa cggttgaatt
    58501 gtctccatac gatcttaacc gtggccgtat tacttatcgc tttaaataat tgtactccgt
    58561 aaaactacag gaggtattat catgaaagta agaccatcag taaaaccaat gtgcgaacat
    58621 tgtaaagtaa ttcgtcgtaa aggacgcgtt atggtgattt gcccagcaaa tccaaaacat
    58681 aaacaacgtc aaggataatt ggaggtgcta taaatatggc tcgtattgca ggagtagata
    58741 ttcctcgtga taaacgtgta gtagtttcac ttacttatat ctatggtatt ggtaacacta
    58801 ctgctaaaaa gatcttagct gatgctggtg tatctgaaga tgttcgcgtt cgtgaattaa
    58861 cgaatgaaca aacagatgct atccgtgcag aagtagataa attaaaagtt gaaggggatc
    58921 ttcgtcgtga agtgaacttg aacatcaaac gtttgatgga aatcggttct taccgtggaa
    58981 tccgtcatcg ccgtggattg cctgttcgtg gacaaaacac gaaaaacaat gcacgtactc
    59041 gtaaaggccc agctcgcgct atcgctggca agaaaaaata attcttaagt gaaggaggtt
    59101 aaaacttcat ggcagcaaaa aaagtgaatc gtaaacgccg tgtgaagaag aatatagaag
    59161 caggtattgc acatatccat tctacattca ataacactat catcatgatc actgatactc
    59221 acggaaatgc attagcgtgg tcatcagctg gttcattagg tttcaaagga agcaaaaaat
    59281 caactccttt tgccgctcaa atggcagcag aaactgctac aaaagcagca atggaacacg
    59341 gattaaagac tgttgaagta acagttaaag gacctggttc tggacgtgaa gcagcaattc
    59401 gttcattaca agctacaggt ttagaagtga ctgcaattcg tgacgtgact ccagttcctc
    59461 ataatggtgc ccgccctcca aaacgccgtc gtgtttaatg agtgccgctc attttgagtt
    59521 ttatcagtaa cgtcattgaa caagacactt ttcgttttga aaggggtaaa gataagaatg
    59581 attgagttcg aaaaaccaag aatcgcaaaa attgatgaag aaaaagatta tggcaagttc
    59641 atcgttgaac ctcttgaaag agggtatggg actactttag ggaattccct acgtcgtatt
    59701 ttattatctt ctttgccagg tgctgccatc actaatattc aaatcgatgg tgtactgcat
    59761 gaattctcta ccatcaaagg tgttagagaa gacgtcactc aaatcatctt gaacattaaa
    59821 ggcttagcgc tgaaaatgta tggccaagaa gaaaaaaccc ttgaaatcga tattaccgga
    59881 cctgctacag taactgctgg cgatattatc gttgatagtg atgtagaaat tcttaacaaa
    59941 gatatgtaca tctgtagtgt ctctgaagga gctacgttcc atgctcgctt aacagtgaaa
    60001 cctggacgtg gttatgttca agcagatgaa aataaaaaag aagatatgcc aattggcgtt
    60061 cttccagtcg attcaatcta cacacctgtt cgtcgtgtga actatcaagt tgagaacaca
    60121 cgtgttggac accgtgaaga ttttgataaa ttgacgatgg agatttggac agatggttca
    60181 attgaaccgt tggaagcaat gagtttgtct gcgaaaatca tgactgaaca tttggatatt
    60241 tttgtgaact taactgatga agcaaaaaat gctgaaatca tggtcgaaaa agaagaaaca
    60301 caaaaagaaa aaatgttgga aatgacgatc gaagagttag acttgtctgt tcgctcttac
    60361 aattgtttga aacgtgcagg aatcaatact gtacaagaac ttacaaataa atctgagcct
    60421 gaaatgatca aagtccgtaa cttaggacgt aaatcattag aagaagttaa agcaaaattg
    60481 cacgacttag gtttaggttt acgtaaagaa gattaaaacc ttttacgaag gagggaaacc
    60541 aacgtgagtt atcgtaaact aggacgcaca tctagccaac gtaaagcgat gttgcgtgat
    60601 ttaacaactg atttattaat caacgaacgt attgtgacta ctgaagctcg tgcgaaagaa
    60661 gttcgctcaa ctgcagaaaa aatgatcact ttaggtaaac gcggagattt gcatgcacgt
    60721 cgtcaagctg cggctttcgt acgtaatgaa gttgccagcg tgcgtgaaga aaatgaggac
    60781 atcgtcattg aatcagcttt acaaaaactg ttcaacgata ttgcacctcg ttacgcagaa
    60841 cgtcaaggcg gatacacacg cattttgaag acagaaccaa gacgcggtga tggtgcacca
    60901 atggttgtct tagaacttgt ctaaaatcac actaataaca tcactttata gatgattctt
    60961 ttagagtgtt atgatgttgg aagatatttt tccgagtcta gctcagcgct gctcctcaag
    61021 cgaatatcgc ttggggagta agcactcatc ataaagtgtt tttttatacc ttcaattacc
    61081 tgtcatgaca gatatgtttg aaggcataaa aaaacatttt aagcgagaaa taattgaaaa
    61141 ttttgtcttt ttttgatagt gttagtagga actttaggat gaagaaggag taatcatgga
    61201 gccaattatt gaattagaaa agataaatta taaataccag ccagatgatc ttcgccccgc
    61261 attgaaagat gtctccttta caattgataa aggtgagtgg atcgctatta ttggacacaa
    61321 tggttcagga aaatcgacac ttgccaaaac gatcaatgga ttgcttctgc cagaatcagg
    61381 gatcgtgaaa gtgggaaatc aaatattgga tgaagaaaat atttggacga tccgacagat
    61441 ggttggaatg atttttcaaa atccagacaa tcagtttgtt ggttcgacag tagaagatga
    61501 cgttgctttt ggtcttgaaa atcaagggat tcccagagaa gaaatgctag tgcgtgtgaa
    61561 agatgcactt gagaaagtta gaatggctga gtttgcttca cgcgagcctg ctcgtttatc
    61621 aggagggcaa aaacaacgtg ttgccattgc cggcgtggtt gccttacgac ctgatatcat
    61681 tattttagat gaagcaacca gtatgctaga tccagaaggt cgtgaagaag tgatctctac
    61741 gatcaagaag atcaaagaag aaagtcaatt gaccgttata tctatcactc atgatattga
    61801 tgaagcagct aatgccaatc ggatactagt gatgagacaa ggcgaacttg tccgtgaagg
    61861 tacaccaaaa gaaatttttt ctgctggtcc tgaactgatc gacttaggtc tagatctacc
    61921 atttcctgaa aaactgaaga gcgcactgaa agaacgtggg gttgatgtgc ccagtgaata
    61981 tatgacagaa gaaaggatgg tggattggtt atggacatcc gttttgaaca agtagacttc
    62041 acttatcagc cgaatacacc atttgaacaa agagccttat ttgacatcaa tatgacgatc
    62101 aaagaaaaca gttatactgc attagtaggg cataccggaa gcgggaaatc aaccttactt
    62161 cagcatttga atgcgcttgt taagccaaca agcggtacag tccatattgg agaacgagat
    62221 attcagccgg atacggataa taagaattta aagcccatcc gaaaaaaagt tggcatcgtc
    62281 tttcagtttc cagaagcaca gctattcgaa gaaacggtag caaaagacat tgctttcggt
    62341 cctaaaaact ttggtgtcag cgaagaagaa gcactagtcc tagcaaaaga aacattagaa
    62401 caagttgggc tggatgaaag ctatttggaa cgttcgccat ttgaactttc aggagggcaa
    62461 atgcgtagag tagcaatcgc tggtgtgctt gccatgaggc cggaagtact tgtattggat
    62521 gaaccaacgg caggacttga tccccagggc agaaaagaaa tgatggagat gttttggcgt
    62581 cttcataagg aacagcagat cactattgta ttagttacgc atttaatgga tgatgtagcc
    62641 aattttgcag attatgtcta tgtgttggaa aagggacgaa tcgttaatag cggggagccg
    62701 caagaggtct ttcaaaacat tgaatggcta aaagaaaaac aactaggcgt acctactgcc
    62761 accgagtttg cagaagaatt aatggctaaa gggatgaact ttgctacctt gcctttgaca
    62821 gctgaagaat tagcagacgc tatcgtccat tatgctggag gaaatgccca tgatgaataa
    62881 attgatattt ggtcgttata tgcctggaga ttccttgatc catcgattgg atccccgagc
    62941 taaattatta gccagttttt attttatcgg aatcattttt ttagcaaata attgggaaac
    63001 gtaccttttg ttggcatttt tcacattact ttctgtcttc ttgtcaaaag tagatttgaa
    63061 attttttatc cgaggcattc gcccattgat ctggctaatc ctcttcactg tagctttgca
    63121 ggtcttgttt acaagtggcg gagaaactta ttggagttgg ggtgttttcc atatcactga
    63181 atttggtgtg atgaacggcc tctttatttt ctgtcgattc gtgttgatca tttttatgtc
    63241 gactctactc acgcttacta caccgccttt ggaattatcg gatgcgattg agtacttgct
    63301 tcgtccattg aaagccgtac ggttcccagt ccacgaaatt tcattgatgc tgtcgattgc
    63361 tcttcgtttt gtgcctacat tgatggacga aacagaaaag atcatgaacg ctcagcgcgc
    63421 tcgcggtgtt gattttggtg aaggaaacct agttcagaaa atgaaagcag tcattccttt
    63481 gctgattcct ttatttgtca gcagcttcaa ccgtgcggaa gatcttgcga ctgctatgga
    63541 agcaagaggc tacaaaggtg gggaaggaag aacgaaatat cgtgtattgc attggcataa
    63601 tagagacacg atggttatca ttgcttttgt attattaact gtgctactga tctttttaag
    63661 aggataaacg ttcagtgggc cgctatctat tcttttcttg ctgatctgtt tgcaagaaag
    63721 gatgcaagca gtgtatgaca agaaatatta gggtctgtgg cagactaagg tcacaggcct
    63781 ctttctttta tctgaaaaga aagaacgaaa aaattttata gaaaaatcat tattccagat
    63841 aaccttttag gaacaaagga gagaaaaaca tggtacgtta caaagcaatc attgcgtacg
    63901 acggaacaaa ttttaatgga ttccaaaaac agccaaatgg gcgtactgtt caagaagaag
    63961 tagaaaaaac attacaaaaa atggcaaatg gaaaagaaat cacggtcttt ggttcgggaa
    64021 ggacagatgc cggagtacat gcgatcgggc aagtaatcca ttttgattat ccagaggagc
    64081 gtccgttaga acgaatgcgt ttcgcgctag atacccagtc accagaggat attgctgtaa
    64141 gacaagtaga aattgtttca gaagacttcc acgcaagata tttggtcaaa gaaaagacgt
    64201 atcaatttcg agtggatata gggaaaccac gcagtccttt tcgacgccat tatgcaagtt
    64261 attttcctta cccacttgat ttggaaaaaa ttcagcgggc gcttcctgat ttattaggga
    64321 ctcacgattt cacctctttt tgtgcatcag gaagctcgat agaggataaa atacgtacga
    64381 tctacgaggc taagatggag gtaaatgaaa caaaagacga attgttgttt acatttaggg
    64441 gaaatggctt cttgtataag atgatacgga tcttagtagg aacgcttttg aaaattggca
    64501 atggccgatt gcctgaagat agtatcccag atattattgc gaaaaaagat cgtaatgcag
    64561 caggacctac agcacaccct gaaggacttt atttgtatga agtgaaatat gaataagagg
    64621 ttatgataga agtggtcagc ttcaagaaat aagaaggtat ttacgaaaat tacttctcaa
    64681 aattttgcaa aatttcggct tattttcgaa gaagctactt ctggaacacc gtttaagaaa
    64741 gaggtcgtga gcctgtcatt taactctgag tcacaaagag caatttcaaa aaacagcttc
    64801 tcatctttga tgaatatcgt gacttgtgga cgaggcgttg ataagtgaat catctttttg
    64861 ttacctattt tttctgtaac caaaaaacga tggaacgttg ttttgattgc ccttggtgcg
    64921 gacagtctga acaggttcca tcgtttcttt attttctacg tcttcttctg tgtgtgtttc
    64981 gtggttgttc ttctattgct ggatcaatcg tgccataaaa ggggcgattg cggtcaaggt
    65041 accagacata aagaataaac aatgcaatac ataggacaaa aaggattgca cctatcgtaa
    65101 atccttcagg caagtaactg aaagtgacag tatgtttacc tgctggaact tttatcatca
    65161 agaatgcgtc tttgaaattg ctgaccttga ctggtttccc atcaatttta gctttccatc
    65221 ctttgtcata aggaatagtt gtcaccaaca tggaatcttc agcggtattt acagtcccag
    65281 aagcttttct gccagtggct tccatttcga cacctttttc tttgatctga ttcattgcgg
    65341 cttgataggc tacaacatct agaccaacga ctttaggctc catgaaactt atttctttcg
    65401 tcccataaaa acttgcagta aatgtcactg ttgtctcttg aggataatag ccaagatcat
    65461 agtattgacc agaaatgttg atttgcgttt ttcgcgatgt accattgaca gtgatcgtcg
    65521 cactagaact ttctaattgc gcaaaattgg ttgggaacaa acttaagtaa gcttgtgtat
    65581 ttgcaggcac atggacttcc caagtgagct cttttggctg gttatctgct gtttctgagt
    65641 aagtgacgcc agtcgttgtc tttgtgatcg tcgtattcaa ctgattcttc aaagtaggtg
    65701 tatagaatgt aaaatactgt tcatttagac cagacaatgt attgatcaag tttgtctgac
    65761 tggtcaaatt atctgtcact ggctgctcaa catcattaac ggacaacggt gcagtaaatg
    65821 ctagtggcaa tgcattgaca ttctgataca gtgtatagtc gccgctttga ccagcttggc
    65881 ggaagccata tttactgaac gtgctttcgt ttttagaaat gttatatttg attcctgtaa
    65941 acccatccat cagcaaagta ttattgttat aacggatatt caaatttgtc cctcttgaac
    66001 ggaaacctaa tttatccagg taagaggaag aattacgatt tcttatagaa gaaaacaaac
    66061 tgataccact gtaaccatag ttgatcgaat cgtttgcaga tactggatcc agattttcca
    66121 agcggtaaaa cgagtcggaa tctttttttg tttcatttac aagagctttc aagtcaggat
    66181 aaggatcagt atataaactg cgggaagcat aattccaatc atcaagaatg ccatggatca
    66241 tactgttcgt attgaccagt gcttcagcag acatgatcaa taataacaat atactcagat
    66301 gattgaatga aagtttttta tgctgataaa atcccacagc aaaagcataa atccctaaga
    66361 aaaccactgt caatacaaag gaagtcatcg tcacataggt gtagcttcct tcaggtttga
    66421 tgccccatgc caccgcaaag agagcagcta cgaccaaaat gattcctaac aacagcccac
    66481 catcgtcttg ttctatcttt tcccatcctt ttcctgcaag caggagcact aagaaagaga
    66541 acagataggc ataacggaat aaaaacatat tcggtgcatg cataccatgc cagaacaaat
    66601 tcaatggaac aagataaaaa cttgcgataa gtatggcgaa aagactgccg tacaaaaatt
    66661 tatttttcaa cggtactttc cgactggcaa aataaaacag acagaggact aatggtagca
    66721 gacctgcgta aacgaacgga atcgatccgt attttgtcgt gtcataaaca ccaatcatat
    66781 ttttcatgat tagatccaga taagaagtcg cttctgtttt caacgtagtg atctgactca
    66841 gtgcctcgcc atttgaccga agatccagca ctgctggcaa tacaatgatc attgaagcgc
    66901 cgcctgccag taaagaagtg ataccataag gaacaatcgt ttttttatat tctgaccagt
    66961 ttgtcaatag acgggcgata aaatataaga aagaaaatac gccaatcata aaacccatat
    67021 aaaaactgga taaaaacaag agtaaataac tgataaataa aagtttcggc cgtttaagat
    67081 ccataacacg atgaatcccc aaaatcacta atggaagata aacaaatgca tccagccaca
    67141 tgatgatctc cgaatgtgca gtgataaatg agatcaatgc gtaactgacg ctaagtgcaa
    67201 catgccccca gcgaggaatc ttgaacgtac gtgcaagaaa ccaaaaagcc aatccggcac
    67261 agccgacctt aagcaaagtc agaacataca gtgcatctgg catcatctgg tttggaaaaa
    67321 agaacaccag tggcgtgaag atcccgccta gatagtaaga aattaaagat aaataattca
    67381 atcctagaga agcattccat gtataaaaaa tactttgctt tccaagaaga acatttcgga
    67441 agctggcatg aaaattagaa aactgtgaaa aggcatcact tgccaaaata cttctgctgc
    67501 ttccaggata gattccgata cttagataaa caagtgctaa aatcaaaaac ggaatcagga
    67561 aactagctgc tgcgtagcgt ccgttttctc taaagaatga ctttaaattt tttttcatat
    67621 ttattttcca ccaaatttct atgaagttgt attcagttta tcatatcctt gtttttctct
    67681 gcttaaaaag agtctagttt tcacaaaaaa ataaaattta gaataaataa cttcataatt
    67741 tgttcgtata acaaaaataa acgcaaaaaa agggtaggaa tcaaaagtcc cacccttaaa
    67801 aatttttatt aaataaaaaa ccgttcacta tctgaacggt ccaagtatca ttcaattaaa
    67861 cgcgttcaac ttttcctgat ttcaaagcac gagttgacac ccaaactttt ttaggttttc
    67921 cgtcgaccat aacgcgaact ttttgtaagt taggtttaac agtacgtttt gttgagttca
    67981 tagcgtgtga acggttgttt ccactttttg tcttgcgacc tgtaaagtaa caaacttttg
    68041 ccatggtaat ttcctccttt gccttgattt ggagcaatag aataattcag ctcataccag
    68101 actaacatat catatagaat gggaatttgc aagtacattt tcatagatac aattgacaat
    68161 tgagaagaat aattgtaaat taaacaaggt tatacctgaa attttgcaag gtttttgagt
    68221 actttgggta actgcctttt atttcaagtt gcgctatgat aaaatatgat agaagaaaaa
    68281 tgggagtttt ttacccgtta aggaggctca ttcatggctg taaaaattca aacgtcagct
    68341 ggaaccattg aaattaccaa tgatgtaatt gcaacagttg ttggtggcgc cgcgacagat
    68401 gtgttcggtg ttgtaggaat ggccagcaaa aatcaaatta aagataatat tactgagatt
    68461 ttgcgtaaag aaaattactc aagaggagta gttgtccgcc aagaagataa tggtattgct
    68521 gtagatgttt atacgattat cagctatggt acgaaaatct ctgaagtttc ccgcaacgtc
    68581 caagaaaaag tgaaatacaa tctagaggca ttattaggcg taactgccaa ttcagttaat
    68641 gtctttgtac aaggtgttcg tgtactgcca gactaggcag gagactggct ggtaactcag
    68701 cagtcgatct gaaggaggat tatctaggtg agtgtaacag aaataagtgc aagccagttc
    68761 caggaaatgg tccaggcagg tgcgaatcgt ctgcaagtaa atgcagaata cgtcaattca
    68821 ttaaatgtat tccctgtgcc agatggcgat acgggaacaa atatgaatct atctatgact
    68881 agtggagcta aggcagtagt agattcaacc tcagaaaaag taggagaatt agcagcaatg
    68941 ctttcaaaag gattgctgat gggtgcgcgc ggaaattccg gcgttatttt gtctcaattg
    69001 tttcgtggat tttctaagca aattccagat gttgtctcat taaatgcttc tgatcttgct
    69061 gcagccttca ctcatggagt tgaaactgcg tataaagcag taatgaaacc agtagaagga
    69121 acgattttga cagtagctag agaagcagcg aaagctggag aaaagaaagc aaaaagtact
    69181 gatgatgtcg ttgaagtaat gacagctgtt gtaaaaggag caaagcgtgc tttagctaaa
    69241 actccagact tgcttccagt cctaaaagaa gtcggtgtcg tagacagtgg agggcaagga
    69301 ctgctgtttg tttatgaggg ctttttaagt gcattgaatg gagaattcca agcggatgaa
    69361 acatatgaac cttctccagc agaaatggat gatatggtca atgcagaaca tcatcgcagt
    69421 gtccaaggtc aaatggcgac agaagatatc cattttggtt actgtacaga aatcatggta
    69481 aaaatcggag aaggacctac agtcgatagt acattcgatt acgaagaatt ccgtaactat
    69541 ttagatggta taggagattc tttacttgtg gtcaatgatg acgagatcat caaagtccac
    69601 gtgcatacag aaaacccagg agaagtcttg aattatggac aaaaattcgg ttcattagtc
    69661 aaaatcaaag tcgacaacat gcgcttgcag catgagacga ttttggaaca cgatgaagaa
    69721 gtgaacgagt ttgcgaaaaa agaaatacca cgtatcccgt atggtatcgt agcgatcgct
    69781 gctggtaaag gcgtccaaga gctgtttgaa agtttaggcg ctaattatgt tatcagtggt
    69841 ggacaaacaa tgaatccaag tacggaagac attgttaaag cgattcaaga agtaaacgca
    69901 gataaagtga ttgtcttgcc aaacaataag aacatcttca tggcagctga tcaagcagca
    69961 gaagtagcag atatcccagt agcagttgtt ccttcaagaa cgatttccca aggattgaca
    70021 gctatgcttt cattcaatga tcaagcttct ttagaagaaa acaaagaaac gatgacagaa
    70081 atgttgtcta gcgttgtgag cggtcaagtc actcacgcga tcagagatac ctcaattgac
    70141 ggtgtagaga tcaaagaagg agactttttg ggcatgatcg atggaaaaat cctgatctct
    70201 aacccagata tcacagcaac agcgttaagt acactagaac aaatgatcaa tgaggacaca
    70261 gagatcgtaa cgatcttggc aggtgatgaa ggaacagttg aacaagcaaa tgaatttgct
    70321 gagaaactaa cagaaaaaca tcctgatttg gaaatcgaga tccaccaagg agatcagcct
    70381 gtctatcctt atctattttc tgctgaataa tttctgtgtc agaatatctt tctaacacag
    70441 tattcattca taaaaggcct tcggttagtg atcaggtatg atcgctgacc aagtcctttt
    70501 tttgttaaag gagggaggaa aaatttatgc gttctttaga tgatccaatc actatgctgg
    70561 caggagtcgg agaaaaaaga gcagctagtc ttgccagctt gggcatccaa acgattgaag
    70621 acttactgac ttattatcct tttcgctacg aagatattca ggaaagaaat ctaaacgaga
    70681 tccaggatca agaaaaggtt actttgaaag gaatcgtagt ctcgcctcct gtgatgaatc
    70741 gatttggtta taaaaaaagc cgattgcaat ttcgtatgat gcaagaacat gatgtgttca
    70801 acgtttcttt ttttaatcaa ccttatctaa aagaaaaagt cattttatcg gaggaaatcg
    70861 ccgtatacgg taaatgggat gcaaagcgta aagccttgaa cgggatgaag attttaggtt
    70921 ctcaaacaat ggatgacttt tcgccgatat atcacgtcaa caaatcaatt cgtcaaacga
    70981 cattagttga tctaatcaaa aaagggtttc aagaatacgg cacattgatt gaagaaaatc
    71041 tgccaaacaa ccttgtggag aaatatcgac tgcttgaccg agcttctgct gtaagaagta
    71101 tgcattttcc aaaagaccac gaagaaaacc atcaagccaa acgacgtgtc gtttttgaag
    71161 agttcttttt atttcaaatg cgtcttcaag gattgaaaaa agcagaaaaa gcagaaacga
    71221 atggtttgga gatcctttat gatgtccaaa aattgaaaca atttactcaa aagctacctt
    71281 ttgaactgac aaatgcacaa aaacgtgtaa cgaatgaaat ctgtcgggat cttcgcagtc
    71341 cgcagcatat gcagcgactt ttgcagggag atgtaggtag cggaaaaaca gtggttgccg
    71401 cgattgcgtt atacgctacc gttacagcag gtttccaagg tgcgttgatg gtgcctacag
    71461 aaatcttggc acaacaacat atggaaagct tgcaacagct gtttgatccc aatgaagtaa
    71521 ccatcgcatt gattacgggg tcaacgaaag caaaagaacg acgtgagctc cttgaaaaac
    71581 tagagcaagg ggaaattaac gtcgtgatcg gcacacatgc attgatccaa gatggagtcg
    71641 aatttcagaa tttaggtcta gttattacgg atgagcagca ccgttttggc gtgaaccagc
    71701 ggaaagtact tcgtgaaaaa ggctggcgac cagatgtctt atttatgaca gcgactccga
    71761 ttcctagaac attagctatt acagcatatg gtgagatgga tgtatctatc attgatgaac
    71821 ttccagctgg acgtatcccg atcgaaacac gctgggtaag aacacctcaa ttggacagtg
    71881 tactggattg gacgaaaaaa gaactggcaa gaggacatca aatgtatgtc atctgtccgc
    71941 tgattgaaga atcagaagca ttagatgtaa aaaatgcagt ggaaatctat gaaaaacttc
    72001 gtgaactatt tgcaccaaaa tatgaagttg gcttgttaca tggtaaaatg aagaatcagg
    72061 aaaaagaagt aatcatggaa gccttcaaag acaaccagat gcagatatta gtttcgacta
    72121 ctgtaattga agtaggggta aacgtgccga acgcgaccgt catgctgatc atggatgcgg
    72181 atcgatttgg attggcacag ctgcaccaac ttcgtggacg tgttggacgc ggaagtgatg
    72241 cttcctactg tatcttagtt gctaatccta aaaatgaact aggtgtggaa cggatgaaaa
    72301 tcatgacaga gaccaccaat ggttttgtac taagcgaaaa agatctggaa ttacgtggtc
    72361 ctggtgaagt atttggcttc cgtcaatctg gtctgcctca atttgcgatt gctgacttgg
    72421 tgacggatgg aaatgttttg gaggtagcca gagaagaggc tgcagccctt tggcaaataa
    72481 aggattggca gatgcttcct gagtatcagc ctttagcgca aagcctccaa atagaaaatc
    72541 aggataatcg ttatttcgat tagcagactg tgctatactt tatactgatg acggaaaaag
    72601 gagtggaaac aatgaaaatt gccgtagatg caatgggcgg agatcacgca cctcaagcaa
    72661 ttgtagaagg cgtggcttta gcgcagcaag attttccaga agtagaattc ttactttacg
    72721 gaaaagaagc agaaatcaag aaatacttaa caaatgaaaa aaacatcacg attatccata
    72781 cagacgaaaa aatcaatagt gatgatgagc cagtaaaagc aattcgacga aaaaagactg
    72841 catcaatggt ccttgctgca caagcagtga aaaacggtga agcagatgct gtattttcag
    72901 cagggaatac aggcgcgcta ctagcagcag ggttgttcat cgtaggacgt atcaaaaata
    72961 tcgaacgccc tggcttgatg tctaccttgc cagtagtagg gaaagaaggt gccggtttcg
    73021 atatgctgga tcttggcgct aacgcagaaa acaaaccaga acatttgttg caatacggta
    73081 ttttaggttc tttttatgca cgtaaagtcc gaggtatcgg acgtcctcgt gtcgcactct
    73141 taaacaacgg aacagaagca acaaaaggta gtgaagtgac gaaaaaagcc tatgaactcc
    73201 ttcagaatga aacaagcttg aattttattg gaaacgtaga agctcgtgag ctactcaatg
    73261 gtgtggctga tgtagtggtc acagatggat ttacaggcaa cgctgttttg aaatctattg
    73321 aaggaacagc aatgaatatg atgaatctat taaaatctgc tattctaaat gaagggatca
    73381 aaggaaaaat gggcgctatg cttttaaaag acggcttacg ttctttgaaa gcagaaatgg
    73441 attattccaa acatggcggt gccgttttgt ttggcttgaa agcaccagtc attaaaactc
    73501 atggagcaac aggccccgat gctgttcgct atacgatccg ccaaatccac acaatgcttg
    73561 agacagatgt agttggacaa ttagtcgaac aatttgaaaa aagcgaagaa tagtatttgg
    73621 atatctgaag cgatccgttc cattgaaaaa aaggtggaca ttcctgaaaa atgccagtaa
    73681 aattagccat ggtgtaggta attgacaagt gtatgattct tggaggtgaa aaagttgact
    73741 cgcgaagaag tatttaacaa agtagcgaaa attatttcaa atcattttga gatagatacc
    73801 gataaagtga cggatgaatt gaacatcaaa gatgatttaa aagctgattc aattagtatt
    73861 atggaattcg ttttagagct tgaagatgaa ttcggtacgg aaatttcaga tgaagacgct
    73921 gaacaaattg aaacagtcgg tggcgcagta gattatatca gcagccatct taaataattt
    73981 caaataaaag caaagaagga taaaaagctg catgcagctt tttatccttc tttttttaat
    74041 ctttttgaca cgtaaaagtc atttttctga taattctggc gataaacaga aattatgttg
    74101 caaagtaaag cggttttcta tataattatt aataattcat tacacaaaga taagaataac
    74161 ctttgttata aaacgattta ttctgtgtaa tgtccttttt tattaagaaa aacttccatt
    74221 taggaagcag agaggggtat tcgattgtcc gataaccaat tattagaagt tcagaaccta
    74281 catacaggat tccgactcaa agatgactat tacgatgcag tagatgacgt ttcatttact
    74341 ttagacaaaa acgagatttt agcaatcgtt ggggaatctg ggtgcgggaa aagtaccttg
    74401 gctactacga ttatgggatt acttgatcct aataacacaa aaattacagg tgagatttta
    74461 tataacgatc tgaatttaat cgacctgaac gaaacgctct ataataaaat ccgcggaaat
    74521 gatatcggaa tgattttcca agatccgttg tctgcattaa atcctctcat gagaatcgaa
    74581 gaccagatca aagaaggact ttcctatcac acaaagatga atgcagaaca gcgtcaagct
    74641 cgtgcactag aattgctaga acaagtaggg attcctaatc ctgcgagagt tggacgccaa
    74701 tatccccatg aactatcagg cggtatgaga caacgtgtca tcatcgcaat cgcaatcgcc
    74761 tgcaaacctc caatcgtcat tgcagatgaa ccgacgacag ccctagatgt aaccatccaa
    74821 gcacagattc ttgatttact gaaagacctt caggaagaaa caaaaacagg gatcatcttg
    74881 atcactcatg acttaggagt cgtagcagaa atggctgatc gagtggcagt gatgtatggc
    74941 ggacagttcg ttgaagtagc aggtgtaaaa gaactgtttg atcatcctaa gcatccttat
    75001 acacgctctt tactgaactc tataccacaa gaaggaaatc atgatgcaga actccatgtg
    75061 atcgaaggtg tcgttccttc tctgaaaaac atgcctcgta aaggttgtcg gtttgcacca
    75121 cgtattccat ggataccagc aagcgatcat gaagaagagc ctatccttca tgaagtcgca
    75181 ccgaaccact tggtacgttg ctcctgctat aagcatttcc actttagaga cgaaaaagga
    75241 gaggtgtaga tgacagaatt aatcacaatc aaagacctga aagttcatta ccctattcgc
    75301 agtggattct tcaatcgagt aacggatcat gtatatgctg tagatggtgt ggattttatt
    75361 atcgaaaaag gaaagacata tggtttagtg ggagagtctg gctctggaaa atctactacg
    75421 ggaaaagccg ttgtgggatt agaaaaggta acagccggac aaatcatgta tgaaggaaaa
    75481 gatgtaacga agcgaagcaa ccgcaaaaaa atgggttata acaaggatgt gcagatgatc
    75541 ttccaagatt caatgtccag tttgaatcct aaaaagcgag tgttggatat cattgctgaa
    75601 cccatccgaa attttgaacg gttaagcgat caagaagaaa agaaaaaggt aaaaggactg
    75661 ctagatatcg ttgggatgcc ggaagatgcg ctatataaat atcctcatga attttcaggc
    75721 ggacaacgtc aacgtctagg agtagcccgt gctgttgcta ccaatccaaa actgatcgta
    75781 gccgatgaac ctgtatctgc tctggatcta tctgttcaag cgcaagtatt gaatttcatg
    75841 aaacgaatcc agcaagaata cggcctcagc tatttattca tttcacatga tttaggtgta
    75901 gtaaaacaca tgtgcgacaa tatcgcaatt atgtacaaag gtcgatttgt agaaatcggt
    75961 acaagagaag acatttataa tgacccgcga catatctata cgaaacgtct gctttcagct
    76021 attccgcaaa tcgatgtaga aaatagggaa agccacaaag aaaatcgacg aagagtagaa
    76081 caagagtata tccaaaacca aaaagaatac tatgatgcga ctggtcgagt ctacgatttg
    76141 cgtacgatca caactacaca caaagtggcg ttgaaagatg gaggtgcaag ctaatgtgga
    76201 aaacgattct gcgacgtgtt ttattgatga tcccgcaagt tattatttta agtgttctga
    76261 tcttcttatt agcccaagcg atgccggggg atccatttac cggtttgatc aatccaaatc
    76321 aggatccggc agtgatcgaa cagatgcgac aagcagctgg cttgaatgac ccatggtatg
    76381 tccagtactt ccgttggatc ggaaatgctt tgcaaggcga ttttggtcaa agtttcttat
    76441 acaaacgctc tgttgctgac ttgatcggcg aacggatcat caacacttta tatctatcga
    76501 tcttgacagt tgtccttact tatttgattg ctgtgcctct ggggatgctt gctggccggt
    76561 atcaaaattc aatcttggat aagattgtcg taatctacaa cttctttagt tttgctgttc
    76621 ctttatttat cttcggcttg atcatgctat ttgtttttgg ttatcgttta ggctggttcc
    76681 caacaagtgg atcacaaaca ctaggtttgc aaggtggatt tatcactcaa tggatagacc
    76741 gtttgaaata tattctgtta ccatcaatta cccaagcatt tttggcgact gccgtcacaa
    76801 tccaatatct gcgaagtgaa gtaatcgatt cgaaatcgct cgattttgtc agaacagccc
    76861 gttccaaagg ggtaccgacg aataaagtat tcagtcgaca tattttccga aatgcagcgt
    76921 tgccgatggc ttctcaaatg gggtatgaaa tcacttcgtt gattgctggt tctgtagtta
    76981 tcgaaaagat ttttggttat ccgggagtcg gaaaactatt tattgattcc atcggccaac
    77041 gggactatac cgtgatcaca tccttagtat tgattttagg aatcgcgacg ctagtgggga
    77101 cattactttc agatatcatc atgagtatcg ttgacccgcg tattcggatt caataaaacc
    77161 aatcagaaag gagaatggat cgatgagtga aaaaaacaat acagcagttc aagaaagtat
    77221 tcctcctatg gggatacgaa tgattgctcg agaatttaaa aaagataaaa ttgctatttt
    77281 ttcattaggt ttgctagtgg ttttattatt agtgatcttt atcggtgcga tgctgaccga
    77341 tcaagacaaa gtgatgtatg ttagtatctt cgacaaatac gctgcaccag gaacagtttc
    77401 gacacaagga caaaagttca ttctgggagc agatgaaggc ggacgtgatg tattaggaca
    77461 attgatcata ggtgctagaa actctgttac gatcggattt gcgatcacga tccttacttc
    77521 gatcatcggg gttggattag ggatcatctc tggctactac ggagggatca ttgacaacat
    77581 cttgatgcgg atcgttgatt ttatcatgat tctaccgatc atgctgatca ttatcgtctt
    77641 cgtatcgatt atttccagat acaatgtctg gtcctttata ttcatcatga gtgcatttta
    77701 ttgggtagct aaagcccgat tattccgaag caagacatta tctgaagcaa gaagagatta
    77761 tgtcagtgcc tcaaaaacaa tgggaaccaa tgattttaaa atcatgttcc gcgaaatcat
    77821 gccgaatcta agttcattaa tcattactaa tttgacgatg aactttgcgg caaacatcgg
    77881 tatcgagaca actttgactt tcttaggttt cggcttgccg gcaaatgtac ctagtttagg
    77941 tacattgatc ggttatgcaa gtaacggaga agtcttagtg aatcggcaat ggatctggtt
    78001 acccgcatca atccttattt tagttttgat gttgagtatc aattatgttg gacaagcatt
    78061 caagcgttct gcagatgcgc gccaacggtt aggctagtat agaagcgaag ggggaaatag
    78121 gaatgaagaa aacgattttt ggatttgcag cgctgatgtc tgctgtcgtt cttgcagcat
    78181 gcgggggcgg aggtacagat acctctggaa acaactctgg aggcaacggc aataacaaag
    78241 aggaattaac ttttccaatt gcaacaaaaa ataatgatga tgcaatcgaa gacggcgaat
    78301 tagaagctgc ggtcgcaacg gacagtcagt ttaaaggatt gttccagtgg gagttttacc
    78361 aagattcata tgatgcagct tttatggctc cttctcatga accgctattt gcaaacgatg
    78421 acgatttcag aatcacagac gacggagctg caacgcttga tctagatgaa gatacgaaaa
    78481 aagcgacaat caaagtaaaa gacaacgtca aatggtcaga tggtgaagat gtaacggcgg
    78541 acgatgtgat tttcccttac gaaatcattg ggaataaaga ttacactggt atccgttatg
    78601 acgacacttt ccgtaacatc gtaggtatgg aagaatatca tgatggaagt gccgacacaa
    78661 tctccggaat caaaaaaata gacgacaaaa cagttgaaat cgaatataaa gaagtaaacc
    78721 caagcatgct tcaatcgggt ggtggtatct ggtcatatgc agcaccaaaa catacgttag
    78781 aaggtgtagc aatcaaagat atggaatcaa gcgatcaagt acgtaaaaat ccagtgactt
    78841 ttggccctta ttacatgagt aatatcgttg caggagaatc agtagaatac ttgccaaacg
    78901 agcactactg gaatggtacg cctaaattga aaaagatcac gatgaaatct gtaccgatgg
    78961 caagtgcaac tgaagcattg aactctaaat tatatgatat gattttccaa atgccaacag
    79021 atacttacga tacctataaa gaaattgctg gctatacaaa cttaggccgt gaacaaactt
    79081 cttatactta tctaggattc aagttaggta aatggaatgc agatacaaag agtgtggaat
    79141 acaatccaga ttcaaaaatg gctgataaat cattacgtca agcaatgggc tatgcattag
    79201 ataataatgc agtaggtgaa cgtttctatg caggtttgag aagcaatgcg acttcattga
    79261 ttcctccagt attccagtca taccatgatg atgagatgaa aggattcaca caagatctag
    79321 acaaagcgaa aaaactatta gatgatgctg gctacaaaga tgtggatgac gatggtttaa
    79381 gagaagacaa agatggaaat aaactaacaa tcaactttgc ttcgatgtcc ggtggggaaa
    79441 cagcgcaacc attggcagat tactatatcc aacaatggaa agaaattggt ttggatgtcc
    79501 aatacacaac aggtcgtctg atcgaattca attctttcta tgataaatta gaaaacgatg
    79561 atcctgaaat tgatgtttac caagctgctt ggaatacagg aactgatcca aatccaagtg
    79621 gactatgggg accaaatgct gcgttcaact acactcgtta tgaatcagaa gaaaatacaa
    79681 aattgatcaa cgatatcagt tcaagcaaag catttgatga agagtaccaa aaagaagcat
    79741 atcaaaaatg gcaacaatat gcatttgaag aagcatttgc gattccaacg ttgttccgta
    79801 atgaagtatt gccagtcaac gaccgtgtca aagactggga ttggtcttat gatgtaccaa
    79861 acccatgggc aaatgtttct gttacagcag attcacgttc ataatcacta aaaatgaccg
    79921 aaaaagggcg atgtcctttt tcggtttttt gatatttttc tgtttatttc tctaatattc
    79981 aggtcaaggc gctgatttca tgacttctcc aataaacggt gttcaaagtc cagtttctcg
    80041 gtattaagtc aaatttatgc aaaaatatgg aaagccattt tcacaaaatt ttttcttcat
    80101 actcgaaact aaacgaattt ttcacaactt ctcgtcaatc actaggatta ttaaagcttt
    80161 acttaccttt tgaccgaacc atttcaaatt tgacaaaaaa ccagtataat aaggaagtat
    80221 cgaaaaaata tccataaaag gaatgtggaa aatggacaac cagctgacaa aagaactaaa
    80281 agaaaactat aacatcgttt ttcacgatct aaatttgctt gaacaagctt ttacccattc
    80341 atcctatgtg aatgagcatc gcaatctgca attatcggat aatgagcgac ttgaattttt
    80401 aggagatgcc gttttagaac tgatggtttc tcagtattta ttccgtttat atcctcattt
    80461 gccagaagga aaattgacaa aaacacgagc tgcgatcgtt cgcgaagaca gcttgtcgaa
    80521 atttgccaaa gaatgtcact ttgatcaata tgttttgttg ggcaagggag aagagaattc
    80581 aggaggacgt acgcgaccag cattgctttg cgacctattc gaagcttttt taggcgcgtt
    80641 atatcttgac cagggattcg acacgaccca tgcattcatc gaaaaagtga tttttcctaa
    80701 agtcaaagca ggtgcttttt cacatgagat ggatcataaa acaaaactac aagaagtctt
    80761 gcaaaaatca ggtgatgtat caatcgaata ccgattgatc aatgaagaag gtccagctca
    80821 tgaacgagta ttttggatcg aagtttatgt agatgatcaa ttgattggca ctggacaagg
    80881 gaaatcgaaa aaattagcag aacaagctgc ggcggaaaat gcattggcag cactgtaaaa
    80941 gtaagtgagg gaaagctttg tatttgaaac gaattgaaat agccggtttt aaatcatttg
    81001 cagacaagac agtcattgat tttgaaaata gcgtcacagc agtggttgga ccaaatggca
    81061 gcggaaaaag caatattaca gaggctatcc gctgggtatt aggagaacaa tctgctaaaa
    81121 gtctgcgagg cggaaaaatg ccggatatca tctttgccgg ttccgatacc cgtaagcagc
    81181 tgaatattgc agaagtcaca gtgattttag acaatagtga tcattatctg ccattggagt
    81241 acaatgagat cagtgtaacc cgccgttatc gacggactgg ggaaagcgag tttttcatca
    81301 ataaacaaag ctgcagatta aaagatatcc aagaattgtt cttagattct ggattaggaa
    81361 aagaatcgtt ctcaatcatt tctcaaggaa aagtagaagc gattttcagt agtaaaccgg
    81421 aagaccgtcg tgggatcttt gaagaagcag ctggtgtctt gaaatacaag cagcggaaaa
    81481 agaaagcaga acagaagctt tttgaaacag aagacaacct cagccgcgtc caagacatca
    81541 tccacgaatt ggaagaacag ttaactcctt tggccgccca gagtgaggca gcaaaggagt
    81601 ttttacggct aaaagaaaca ctgactcaaa ccgatgtctc tttgatggtt gctgaaatca
    81661 agacagcaaa aaaagattgg gacaacaaac aagcacagct tgcgaaattc aatctagaat
    81721 taggcaaact atctgagtcg atccaagaac aagaatccat tttggcgaag cagcgtaaag
    81781 aaaatgcgca agccgatcgt ctgatcgaaa aaaatcagca agtattatta gatctttcgg
    81841 aaaaactgaa gcagacagaa ggacaaaaag atgttttgca ggagcggaca aagcatacac
    81901 aaaaaagctc gcaagaatat caaacttcat tagcagaagc acaaaaaaag gttaaacatt
    81961 ttgaaaagct tcaagaaagc ttgatgaaag cagcagcaga aaaagaaaca gagatccaaa
    82021 aagctgaagc aaatctcata aaaacacagc aagaattaga aaaataccaa aaatcaacga
    82081 aagaattatt ggcagaatta agagatcaat atgttgattt gatgcaagaa caagcagctg
    82141 taggaaacga actgaagtat ctagaacgtc aatatataca agaaacagca aaaagcaagc
    82201 agacgttagc caagcaatcg gaagtagaag catcagtaga ccgattgatc ttacaaaaag
    82261 aagaattgac acaaaagcag gcgcagctaa aatcatcttt aactgaaaca aaagagaagt
    82321 tagaaatgat ccaacaaaac gggaaaaagt tccaagaagc attagcaaaa gaacagccta
    82381 aaatgtatca attaatgaat caagtccagc agctacgtgc tcgtaagaaa agccttcaag
    82441 aaatccaaga aaactatttt ggtttttacc aaggggttcg attggtcttg cagcataaac
    82501 agcagctttc gggtattgtc ggagcagtgg ctgaactgat cgatgttcct gcagatttca
    82561 cgttagctat cgaaacagct ttaggtgggg cagcccagca tgtcatcgtt gaaaatgaaa
    82621 aagatgctcg gcaagccatc acgtatttga aacagcaaag aggaggaaga gcaacattcc
    82681 ttccactgac gacaatcaaa ccaagacaat taccggctca tatattgaca caagcagcag
    82741 ctgtagaagg gtttataggg attgccagtg aacaagtcag ttatcccgat caaatccaaa
    82801 ctgtggttca aaacctgcta ggaacgatcc ttttagccaa agacttaact agtgccaatg
    82861 caatcgcgca aacgatccgc tatcaatatc gagtggtttc cttagaaggt gatgtgatga
    82921 atgccggagg atccatgaca ggtggagcga ataaacgagg aaaccaaggc agtttgtttg
    82981 tacaaaatca agaattgaaa cagttgacta gtgaatttga agaagcagat aaacaactcc
    83041 aagcacaaga aaaaaaagtt caggagctgc aacaagaaac agctcgttta gccgaagaac
    83101 aagaagtttt gcgtacacga ggcgaacaat tacgttttga agaacaagaa gcgacaaatc
    83161 agctacaaaa catcataaat gaacttgaac gttttgaaaa agaaaagcaa atctcaactt
    83221 ttgagacacg tgaactgcag cagtttatcg aagattacga aaagcaaacg aacgaattga
    83281 aagacaaaca aacagatctt gaatcgcaac gacaaaaaat cgacgaagaa atcaaatcgt
    83341 taagtcaaga aagcgaccag atggaagcca gaagagcaca agtacaaagt caaaaagcac
    83401 aagaacaagc agatcttgct gtcttgaaag agcagtttaa tcatctgcag atccagctcc
    83461 gcggtgcccg agtccaaaaa gcagaggcaa ccgaacgtca agaagcgata gaaaagcagt
    83521 tggcgacatt gacagcagac ttttctgatc atgaagtcac agaagaaagt ttggaaaaac
    83581 aaattaacga attatctgct cagcgagaaa cattaaaagc cgagttggca aaagccaaag
    83641 agcagcgaga tagaaggcaa aaggagatcg ataagcttga agcagtcttg gcagagcgta
    83701 atcgtgagca aaaagcacga ctgtcggaac aatccaaatt agaagtccaa aaagaccgag
    83761 ctgaaatgat tttagataat catttgacct atctgcagtc agaatatcag ctgacatttg
    83821 agaaagcttc ccaagactat caagaaacaa ccgatatcga agacagtaga acaaaggttt
    83881 catccttaaa agaacagatt gaaaaactgg gaccagtaaa cttaaattca atcgaacaat
    83941 acgaacaagt cagtgaaaga catacattct tagcaacaca gcgagatgat ctgttagcag
    84001 cgaaaaacca attgtttgaa acgatggatg agatggatga tgaagtgcgg acaagattca
    84061 aagaagtatt tgaagctatc cgtcaagaat tcaaaattgt ctttccaaat atgtttggcg
    84121 gcggtaaagc tgaattagta ctgacggaac caactgattt gctgaaaaca ggtattgaga
    84181 ttgaagtaca acctcctggc aaaaaactgc aaagtctaag tttgctttct ggaggagaac
    84241 gagcacttac agccatcgcg ttgctctttt ccattatccg tgtctgtcct gttccgtttt
    84301 gtatcttaga tgaggtagaa gcagcgttag atgaagccaa cgtgaaacgt tttggtcgtt
    84361 acttgagcga tttccaagat gacacacaat ttatcgtagt cacacaccgc aaaggaacga
    84421 tggtcgcagc agatgtactg tacggagtga cgatgcagga atccggtgta tcaaaaatcg
    84481 tttctgtacg gatggaagat atcaatgaag aggcgaaaat caacgaaacg aaagtgtagg
    84541 aacgaaaatg atcaaattag tagcaatcga tttggacggt acattattaa atgaacaaaa
    84601 gctgatttca gatgagaata aacaagcatt agcacaagca aaacagcaag gggtcaagat
    84661 cgttctatgt acaggacgcc ccttagctgc gatggcgcat tatctgcaag aattaggact
    84721 ggtagatgaa ggtgactaca gcatcacttt caacggcggt ttggtacaaa aaaatgatac
    84781 tggtgaaatc atggaaaaga aagtgatgga agtatcagat atccaacgct tatacaaact
    84841 ggctcagcaa ttggatctgc cactagatgt tttgtctgac aatgtggttt tacagctgcc
    84901 tagtgcccca aggaagcaat ccttgtacaa tgtgttgaat aagctgctgc aattccaacc
    84961 agcggatata agcgatatta cagatgaatt tgtgcttaat aaggcagtaa tcgcttatga
    85021 ccaagaagaa ttagatccta aaatcaaaga aataccagca gaattccatg aaatgtatga
    85081 gatcatcaaa acaagaagta tgcttttgga atttatgcca aaaggtgtta caaaagctta
    85141 tggtatttct ttattggcaa aagatcttgg tttgaaacaa gaagaaatca tggcgatcgg
    85201 tgatgaggaa aatgatttac cgatgatcca atatgctgga ctaggcgtag cgatggagaa
    85261 tgctgtgcct ttcgtcaaag aagcagcaga tttcgtgaca gccagcaatg aagaaaatgg
    85321 cgtagccaaa gtgatccaaa aatttgtatt agagtagggg gagtcaaatg ggattttttg
    85381 ataaaatcaa gcgtgctttt actggcgaag agaaaacaga agaagttgaa gtacaggaga
    85441 agtacgacaa gggtttagaa aagacgcgta aaacatttgg tgaacggatg aatgagttgt
    85501 ttgccaattt tcgaacagtc gatgaagact tttttgaaga attagaagaa acacttatcg
    85561 gtgcagatgt aggttttgaa acagcgatac gaattaccga agcattacgt caagaagtga
    85621 aactcagaaa tgcgaaaaaa tcggcagaag tccaaaatgc gatcatcgag aaacttgtgg
    85681 atctctatga agcggaagga atcaatgaag taaatgaact aaatatccaa gaagacggac
    85741 taacagttat tttgtttgtc ggtgttaatg gtgtagggaa aacaacaagt attggtaaac
    85801 ttgcccatca gttcaaaaac gaaggtaaaa aagtattgat ggcagcagca gataccttcc
    85861 gcgctggagc aatcgaccag ttagttgtct ggggcgaacg cgctggtgtt gaagttgttc
    85921 gtggcaatgc aggaggcgat ccagcagctg tcgtattcga tgctttggaa agagcaaaga
    85981 cagagcaggc agatgtttta ctagttgata ctgcaggccg actgcaaaat aaagtgaact
    86041 tgatgaacga attagaaaaa ataaaacgag taatccagcg ggaattacca gaagcacccc
    86101 aagaaacact tcttgtatta gatgcaacca ctggtcaaaa cgcgatggtt caagcaaaac
    86161 aattcaaaga aacaacagat gtgaccggtc ttgttttgac aaaattagat ggtacagcaa
    86221 agggcggtat cgtacttgct atccgtaatg aattgcattt accagtgaaa ttggtcggtc
    86281 ttggtgaagg aatcgatgac ttggagccgt ttgatccaaa tgattttgtc gttggattgt
    86341 tcaaaggatt actaaaagaa gaataaccca tgtctaaaaa gcaggagctg tgacaaaagt
    86401 tgcagctcct tgtttgaatg attgttttta acatagctgt gtttttgatg ccttcatttt
    86461 tatacttatt tgtaagcggt tctcaaacaa tttcgttctt tttcttagac tttttgatta
    86521 taattagatt catatgaatt ggaggtcact taatgatgaa ttttaagcaa ttattattgc
    86581 atgtcaatgc gcgccctttt atcgaccaag ctcgatttgg tatagaacgg gaaggacagc
    86641 gggttgatct tgcaggaaat ctagcaaaaa ccgatcatcc agcaatcttt ggcgatcgat
    86701 cctatcaccc ctatatccaa acagatttta gcgaaacaca aacagagatg atcacccctg
    86761 ttaccgattc tattcccgaa ttatttcagt atctcgctgc tgtttatgat gtgactgctc
    86821 gttctatacc gaaagaagag atgatctggc cattaagtat gccacctgcc ttaccggaaa
    86881 aagacgaaga gatcattatt gcaaaattaa aaaatttcga agatgtcttg tatcgacgtt
    86941 atttagcaaa agaatacggg aagcgaaaac agatggtaag cggtattcat ttcaattttg
    87001 aatttggcga tgagttgcta agaacattgt tcagccatca ggaagaattt caagattttt
    87061 ccgaattcaa aacagaactt tatttgaaaa cagcgagaaa ctttatgcgc tatcgctgga
    87121 tgatcactta tttattcggt gcttcaccaa tgagtgagaa gaactacttt ttagatgaat
    87181 cacatccgca agaacctgtt cgcagtattc gcaacagtgc gctgggctat acgaatcatc
    87241 caaatgtgaa agtttcctat gcttctatga aacagtattt agcagatatc gagcgaatga
    87301 tcgaagaagg gaaactttcg gaggagaaag aattttatac accgcttcgt ttccgaggcg
    87361 gaaagaaagt cgcagattta gcaacaacag gtgttcgtta tattgaattg cggaatatcg
    87421 atttgaatcc ttatgcaaga ttgggtatca atccagagca agttcggttc ttacaattat
    87481 tcctgatgta catgttatgg acagaagaaa aagaagactg tgaccaatgg gtagcagaag
    87541 gtacgactcg aaataacaaa gtagcactag aacaaccatc agatcaaaca gaatttcatc
    87601 aagaaggtag agaaattctt gaaggaatga agcaaatgct ggttgaattg gattggttgg
    87661 attctcttta tttagtagag gaagcattga ctcaaatgga tcatcctgaa caaacgttag
    87721 cagcgaaact ctatcaagaa gcacagctat cgagtcagca agaagttgct gtggcattgg
    87781 gacatcaata ttataaagaa agccatgaac gtccctatca gttggccggt tttcgcgaaa
    87841 tggaactttc tactcagata tttatgttcg atgccatcca aaaaggtgtc caagtcaaag
    87901 tattagatga gtcggatcaa tttttgcgtc tgcaattcca agaccatgta gaatatgtga
    87961 aaaatgcaaa catgacaagt aaggacagct atatcgtccc acttatcatg gaaaacaaaa
    88021 cagtaacgaa aaaagtctta aaagaagctg ggttccgggt accaggaggc gcagaatttt
    88081 catccatgga agaagcggta aaagcttatc cgagatttgc cgagcaggca tttgtgatca
    88141 aacctaaatc aaccaattac gggttaggta tcacgatttt caaggaagga gctagtctgg
    88201 aagattatca agcaggttta gccattgctt ttcgtgaaga cagttcggtt ttagtagaag
    88261 aatttatgcc gggaacggaa tatcgtttct ttgtgatcga cggagaagtc caagccatca
    88321 tgttgcgagt ccctgctaat gttattggtg acagtatccg aacagtgaaa gaacttgttg
    88381 aagaaaaaaa tagtgaccct ttgcgtggaa cgaatcatcg tgcaccttta gaattgatcc
    88441 aattaggtga attagaacaa ttgatgttga aagaacaagg gttgacgatc gaatcagttc
    88501 cccaagccaa tcaaattgtt tatctaaggg agaactcaaa tatcagtaca ggtggtgatt
    88561 cgatcgatat gactgatgaa ttttctgaag cttacaaaaa aatcgccgtt tctgcggtag
    88621 aagcactagg agctaagatt agtggtatcg atttgattat accagataaa gaaatcgatc
    88681 ctacaactga taaaaaagca tatgggatca tcgaagcaaa tttcaatcca gccatgcata
    88741 tgcatgttta tccttttgca ggaaaaggca gaagattgac gatgaatgta ttgaaattgt
    88801 tatatcctga agtattttaa aagaaaaagc ttttgacaga ttgtcctatt tgatgtcaat
    88861 ctgatcaaaa gctttttttg catgtaaaaa tttatgtgtt ttttcgtttt aaaataaacg
    88921 atcccagaag ctttttttct tttttggttt ttctttttca gctggcgaag agttttcttc
    88981 tgggtatttt ttttcgatat caatcacagg ttcatttact gcttcttttg ctgttaagag
    89041 caatccgaat tcctcaggat ttgcagtgac gtaatcgttg actacagtga aacggctatt
    89101 gtttttcgtt gctagctgga tatattggtt ttggacagaa aggggtagag ccccattcaa
    89161 gagtatggaa gcatctggat gttttttgag ttcttttaaa aagttttctt tgttttttgg
    89221 ttggatcatt tcagcaacag tcatactaag atagcagcgt tccctgaatg tccctaaata
    89281 tttatgctgt tcttcagggt tcactaaagg tgtaccataa agtcccttat ccaaatggct
    89341 ttttacatca tcttgtgcca taaaaaatac cctccttctt ctctttgtct ttcttacgat
    89401 tatagcatat tcccaataat acctaaaaca tatgaaaaag cggttgcttt tgttcaacga
    89461 ttcacttaaa ataagaatga aaatcattct caattagaaa ggtggattgg atgcgctacg
    89521 gacgactagt agcattatta gttattttaa gtagtatttc attgttcatc ggagtatcga
    89581 atgtacaatt gtcagaactg ctccagttag atgggcaaag cttacaaatc ttgctagtga
    89641 ctcgtttgcc acgaacgatt tgtttgatcc ttgtgggagc gaccagtagt atttgtgggt
    89701 tgatcatgca acatttgacc caaaataaat ttgtttcacc aacgactgcg ggcacgatgg
    89761 atagtgcgcg tttaggtatt ttagtggcta tgattttcct acctggtgca tccttgattg
    89821 tccgatcctt gacagcattt tgctttgctt ttgcaggaac attgcttttt ttatccctta
    89881 cgcgattgtt cctccagaaa aatcaattga tgcttccgtt gattggagtc atgttaggga
    89941 atatcattgg ttcagttgct actttttttg cttatcaatt ccagttggta caaaatatga
    90001 cttcatggct tcaagggaat tttgcgactg tgatacgagg aaattatgag ttattgtatg
    90061 ctgttgttcc attgttgctc ttggtttggc tatttgctta ccgatttacg atcattggtc
    90121 tgggcgaatc gtttgcagta aatctgggaa tagattacca gaagatgcag tttttaggca
    90181 ttggcttagt atcattggct agtgcagtca tgttgatcat ggtaggaagc attcctttta
    90241 tcggagtgat cattccgaat cttgtctcta gaatatatgg agatcaagtg cacaaaacaa
    90301 ttggcgtaac tgctttagca ggtagtttgt tcttagtatt ttgcgatctt gtcgctcgcg
    90361 tgttgatttt tccttatgaa ataccagtca gtgtgatcgt tggaatcatc ggtggggcta
    90421 tcttccttta tcttctgata aggggacgac gatatgctta aaaaaaagcc atctattttt
    90481 attggtatgc ttgttcttgt tttatttgtt acaggaatgt acttgacgat ccatacatat
    90541 ggaaactggc attttgcatg tgcattgagg agtaagaaaa tttttgcatt tatccttgtc
    90601 tcgctagctg tcagcagtgc cacaatcagc tttcagacaa ttacgcataa ccagtttttg
    90661 actccttcag tattgggact tgagtcgtta tatgtccttg tccaaacggt ccttttcttc
    90721 tttatcggag gagtgacgat gcttcagcag acaggtacag tttttttctt attgaacgtt
    90781 gggctgatgg tcgggctgag tctattgtta tttttctttc ttttgaaaaa aatgagagcg
    90841 aatctttttt tattactgat gtcagggatg attttaggta cgctttttgg tagtgtcagt
    90901 acctttttac aagtcatcat ggatccgaat gaatatgatt tgcttcaagg cagattattt
    90961 gcaagctttg gcaatatcca gagtcagtat cttttgcctg cttctttgat cattatcggc
    91021 tgctggtacc tgttatttcg atatcgacat gtgctagacg ttttccatct cggaaatgaa
    91081 caagcagtaa acctcgggat agacacagat cgttttgaat ttttgatttt atgttgtgtc
    91141 agtcttttga cagggacatc aacggctctt gtgggtccag ttactttttt agggtttatt
    91201 gtcgcaaata tcagttatcg tgtcttttct acttatcgtc atggtcagct atttatcgga
    91261 agctttctgg tagctatgct gtttctgaca ggaggacagc tcgcagtaga gcagattttc
    91321 ggctggaata caacagtgag tgtcgtcgtt gaatttgtcg gaggaattta ttttgtcaca
    91381 aaattgattc gcgaaaggaa gagtatccta tgattgattt acaaaaggtc aataaactgt
    91441 accatggaaa atcagtggtt aaagatgtac agttaacgat tcctgaaaag aaattgactg
    91501 cttttatcgg tccaaatggt gctggaaagt caacggtgtt gtcgatgatc agtcgtttga
    91561 taccaaaaga tacaggtgaa atctatttag accataacga agtaaaggct tggaaatcca
    91621 atgaattagc caaaaaatta tcgatcctac gacaatcgaa tgaaataaat gtccaaatca
    91681 ccgtgaaaga attagtagaa tttggacgat ttccttatac gaaaggaaaa ttgacgacaa
    91741 aagacgagga aatcgttcag gaatcactgg aacaccttgg gttagaagaa atagcggatc
    91801 aatcgattga cactctttca ggcggtcaac tccaacgtgc gtatattgca atggtgctag
    91861 cgcaagacac ggattatatt ttactggatg aacctttgaa caatctagat atgaattttg
    91921 cggttcaaat catgcagatt ttaaaaaatt tagtacaaga aatcgggaaa acaattgtta
    91981 ttgttcttca tgatatcaac tttgctgcaa gttatgcaga cgaaattgtt gcaatgaaag
    92041 atggacagtt atttacgcaa ggaacgaccc aagaaatgat aaaaaaagat gtattggatg
    92101 aattatacga aatggacata cggatttgtg agattgaagg ccgcagattc tgtctttatt
    92161 tctaaaacta acattaggag agaagagatg aaaaaattag tagtaggtat cttgattcta
    92221 gcatcatttg gtttagcagc atgtggaaat agcaacaaca ccagtcaggc agacacaaaa
    92281 aaaagcagca cacaaactga aactacattg acgataacag atagtaatgg agaccaaatc
    92341 gaggtgccaa ataatccaga aaaagtcgtc gtattcgaca atggttcttt agatacgatg
    92401 gatgctcttg gtgttggaga taaggtagtt ggagcagcta caagcagcct tcctgagtat
    92461 ctttcatcct ataaaaaagt agaatcagca ggtggaataa aagaaccaga cttagaaaaa
    92521 attaatcagc ttcagccaga tctaatcatc atttctggcc gacaaagaga ttttcaaaaa
    92581 gatctaagtc aaattgctcc gaccattttc ctttctttag atgcgaaaaa tccatgggaa
    92641 tctttccagc aaaatgtcac tgctttaggg gagatctttg gtaagcaaga agaagctaaa
    92701 actcaattag aggaattatc tagtgcaatc gatcagacga aaaagaaagc agaagctact
    92761 gacaaaaaag cactagtcac acttgtgaat gaagggcaat tatcagccta cggatctggt
    92821 tctagattcg gtttcatcca tgatttattt ggctttgaac aagcagatga tcaaatcgaa
    92881 gcatccactc acggtcaaag tgtttcttat gaatatgtgc tggaaaaaaa tccggatatc
    92941 ttatttgtag tagacagaac aaaagctatt ggtggagacg acagcaaaga tgatatttct
    93001 gccaatgaac tggtagcaca aacgaatgct ggtaaaaatc agcagatcat ctcccttgaa
    93061 ccagatgtct ggtatttaag cgggggcggt ttagaatcaa tgaaactgat gatcgaagat
    93121 gtgaatcaag catttaaata gcataaataa cagaggatta aactctcata ctatgtaaca
    93181 ataatgtttc ttacattctt aaatagcaag agcctatcct caaagaagtt atcaaaaacc
    93241 atccgtaaaa tcgtattgat gattttacgg atggttttta tttatttgag aaatcttttt
    93301 tcaacgtgaa ttcattatct acatcatacg ggttgattct agaatgggga gtggcaaaaa
    93361 atagtgtgat ccattttttc agcatcaaga tcgcctcctt tgatttgaat caaaggtaca
    93421 aagcgaaact tagataaaac ttaaatttag atatttttat tttgtaccga tctcttcttt
    93481 aaacgatgtt ccatctgctg cttctcggca ataagtccac tagactagaa aatatgagaa
    93541 gctattttct gtccagcgtt cttattactc a
//
LOCUS       DARBFO010000003        89653 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000003 DARBFO010000000
VERSION     DARBFO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 89653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.003"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(552..2165)
                     /locus_tag="SCK51_000267"
     CDS             complement(552..2165)
                     /locus_tag="SCK51_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1250924.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..3214)
                     /locus_tag="SCK51_000268"
     CDS             complement(2306..3214)
                     /locus_tag="SCK51_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1250925.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3397..4521
                     /locus_tag="SCK51_000269"
     CDS             3397..4521
                     /locus_tag="SCK51_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1250926.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4546..4725
                     /locus_tag="SCK51_000270"
     CDS             4546..4725
                     /locus_tag="SCK51_000270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250927.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            4748..5665
                     /locus_tag="SCK51_000271"
     CDS             4748..5665
                     /locus_tag="SCK51_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1250928.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5658..6491
                     /locus_tag="SCK51_000272"
     CDS             5658..6491
                     /locus_tag="SCK51_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1250929.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6574..7692
                     /locus_tag="SCK51_000273"
     CDS             6574..7692
                     /locus_tag="SCK51_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG1250930.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7686..9584
                     /locus_tag="SCK51_000274"
     CDS             7686..9584
                     /locus_tag="SCK51_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEG1250931.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9578..10801
                     /locus_tag="SCK51_000275"
     CDS             9578..10801
                     /locus_tag="SCK51_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG1250932.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10887..12821)
                     /locus_tag="SCK51_000276"
     CDS             complement(10887..12821)
                     /locus_tag="SCK51_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1250933.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13047..13703
                     /locus_tag="SCK51_000277"
     CDS             13047..13703
                     /locus_tag="SCK51_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEG1250934.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13777..14130)
                     /locus_tag="SCK51_000278"
     CDS             complement(13777..14130)
                     /locus_tag="SCK51_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1250935.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14322..15251
                     /locus_tag="SCK51_000279"
     CDS             14322..15251
                     /locus_tag="SCK51_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEG1250936.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15262..16098
                     /locus_tag="SCK51_000280"
     CDS             15262..16098
                     /locus_tag="SCK51_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEG1250937.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16346..17122
                     /locus_tag="SCK51_000281"
     CDS             16346..17122
                     /locus_tag="SCK51_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1250938.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17298..18041
                     /locus_tag="SCK51_000282"
     CDS             17298..18041
                     /locus_tag="SCK51_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1250939.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18034..19614
                     /locus_tag="SCK51_000283"
     CDS             18034..19614
                     /locus_tag="SCK51_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEG1250940.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19865..20344)
                     /locus_tag="SCK51_000284"
     CDS             complement(19865..20344)
                     /locus_tag="SCK51_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEG1250941.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20360..21112)
                     /locus_tag="SCK51_000285"
     CDS             complement(20360..21112)
                     /locus_tag="SCK51_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEG1250942.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21229..21299
                     /locus_tag="SCK51_000286"
     tRNA            21229..21299
                     /locus_tag="SCK51_000286"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21261..21263,aa:Gly,seq:ccc)
     gene            21660..22385
                     /locus_tag="SCK51_000287"
     CDS             21660..22385
                     /locus_tag="SCK51_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEG1250943.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22640..22906
                     /locus_tag="SCK51_000288"
     CDS             22640..22906
                     /locus_tag="SCK51_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEG1250944.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22903..23334
                     /gene="ruvX"
                     /locus_tag="SCK51_000289"
     CDS             22903..23334
                     /gene="ruvX"
                     /locus_tag="SCK51_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEG1250945.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23359..23664
                     /locus_tag="SCK51_000290"
     CDS             23359..23664
                     /locus_tag="SCK51_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEG1250946.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(23745..24890)
                     /locus_tag="SCK51_000291"
     CDS             complement(23745..24890)
                     /locus_tag="SCK51_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1250947.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(24951..25598)
                     /locus_tag="SCK51_000292"
     CDS             complement(24951..25598)
                     /locus_tag="SCK51_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1250948.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            25792..26085
                     /locus_tag="SCK51_000293"
     CDS             25792..26085
                     /locus_tag="SCK51_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250949.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            26129..26440
                     /locus_tag="SCK51_000294"
     CDS             26129..26440
                     /locus_tag="SCK51_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250950.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            26437..27061
                     /locus_tag="SCK51_000295"
                     /pseudo
     CDS             26437..27061
                     /locus_tag="SCK51_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            27202..27360
                     /locus_tag="SCK51_000296"
     CDS             27202..27360
                     /locus_tag="SCK51_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250951.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            27361..27723
                     /locus_tag="SCK51_000297"
     CDS             27361..27723
                     /locus_tag="SCK51_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250952.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            27760..28608
                     /locus_tag="SCK51_000298"
     CDS             27760..28608
                     /locus_tag="SCK51_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEG1250953.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            28598..30073
                     /locus_tag="SCK51_000299"
     CDS             28598..30073
                     /locus_tag="SCK51_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEG1250954.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            30339..30746
                     /locus_tag="SCK51_000300"
     CDS             30339..30746
                     /locus_tag="SCK51_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HEG1250955.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            30749..30964
                     /locus_tag="SCK51_000301"
     CDS             30749..30964
                     /locus_tag="SCK51_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250956.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            30968..31348
                     /locus_tag="SCK51_000302"
     CDS             30968..31348
                     /locus_tag="SCK51_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG1250957.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            31482..31637
                     /locus_tag="SCK51_000303"
     CDS             31482..31637
                     /locus_tag="SCK51_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HEG1250958.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            31706..32179
                     /locus_tag="SCK51_000304"
     CDS             31706..32179
                     /locus_tag="SCK51_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEG1250959.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            32176..33870
                     /locus_tag="SCK51_000305"
     CDS             32176..33870
                     /locus_tag="SCK51_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG1250960.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            33836..34021
                     /locus_tag="SCK51_000306"
     CDS             33836..34021
                     /locus_tag="SCK51_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250961.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            34025..35200
                     /locus_tag="SCK51_000307"
     CDS             34025..35200
                     /locus_tag="SCK51_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG1250962.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            35193..36716
                     /locus_tag="SCK51_000308"
     CDS             35193..36716
                     /locus_tag="SCK51_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG1250963.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            36772..37056
                     /locus_tag="SCK51_000309"
     CDS             36772..37056
                     /locus_tag="SCK51_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEG1250964.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            37043..37378
                     /locus_tag="SCK51_000310"
     CDS             37043..37378
                     /locus_tag="SCK51_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEG1250965.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            37673..38299
                     /locus_tag="SCK51_000311"
     CDS             37673..38299
                     /locus_tag="SCK51_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250966.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            39041..39878
                     /locus_tag="SCK51_000312"
                     /pseudo
     CDS             39041..39878
                     /locus_tag="SCK51_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            39943..40377
                     /locus_tag="SCK51_000313"
     CDS             39943..40377
                     /locus_tag="SCK51_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1250967.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(40445..41767)
                     /locus_tag="SCK51_000314"
     CDS             complement(40445..41767)
                     /locus_tag="SCK51_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEG1250968.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(41921..43561)
                     /locus_tag="SCK51_000315"
     CDS             complement(41921..43561)
                     /locus_tag="SCK51_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEG1250969.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            43891..44361
                     /locus_tag="SCK51_000316"
     CDS             43891..44361
                     /locus_tag="SCK51_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEG1250970.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            44990..46093
                     /locus_tag="SCK51_000317"
     CDS             44990..46093
                     /locus_tag="SCK51_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEG1250971.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            46377..47057
                     /locus_tag="SCK51_000318"
     CDS             46377..47057
                     /locus_tag="SCK51_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG1250972.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            47147..48181
                     /locus_tag="SCK51_000319"
     CDS             47147..48181
                     /locus_tag="SCK51_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEG1250973.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            48195..48530
                     /locus_tag="SCK51_000320"
     CDS             48195..48530
                     /locus_tag="SCK51_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250974.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            48514..50994
                     /locus_tag="SCK51_000321"
     CDS             48514..50994
                     /locus_tag="SCK51_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250975.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            51050..52609
                     /locus_tag="SCK51_000322"
     CDS             51050..52609
                     /locus_tag="SCK51_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1250976.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            52606..53970
                     /locus_tag="SCK51_000323"
     CDS             52606..53970
                     /locus_tag="SCK51_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1250977.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            54141..55211
                     /locus_tag="SCK51_000324"
     CDS             54141..55211
                     /locus_tag="SCK51_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1250978.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            55226..55894
                     /locus_tag="SCK51_000325"
     CDS             55226..55894
                     /locus_tag="SCK51_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1250979.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            55914..56492
                     /locus_tag="SCK51_000326"
     CDS             55914..56492
                     /locus_tag="SCK51_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1250980.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            56895..57806
                     /locus_tag="SCK51_000327"
     CDS             56895..57806
                     /locus_tag="SCK51_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG1250981.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(58062..58979)
                     /locus_tag="SCK51_000328"
     CDS             complement(58062..58979)
                     /locus_tag="SCK51_000328"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEG1250982.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            59181..59618
                     /gene="zapA"
                     /locus_tag="SCK51_000329"
     CDS             59181..59618
                     /gene="zapA"
                     /locus_tag="SCK51_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEG1250983.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            59676..60223
                     /locus_tag="SCK51_000330"
                     /pseudo
     CDS             59676..60223
                     /locus_tag="SCK51_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            60338..62698
                     /locus_tag="SCK51_000331"
     CDS             60338..62698
                     /locus_tag="SCK51_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEG1250984.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            62785..63099
                     /gene="trxA"
                     /locus_tag="SCK51_000332"
     CDS             62785..63099
                     /gene="trxA"
                     /locus_tag="SCK51_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEG1250985.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            63281..65059
                     /gene="uvrC"
                     /locus_tag="SCK51_000333"
     CDS             63281..65059
                     /gene="uvrC"
                     /locus_tag="SCK51_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEG1250986.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            65204..66103
                     /locus_tag="SCK51_000334"
     CDS             65204..66103
                     /locus_tag="SCK51_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1250987.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            66096..67358
                     /locus_tag="SCK51_000335"
     CDS             66096..67358
                     /locus_tag="SCK51_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1250988.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            67521..69419
                     /locus_tag="SCK51_000336"
     CDS             67521..69419
                     /locus_tag="SCK51_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEG1250989.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(69718..70437)
                     /locus_tag="SCK51_000337"
     CDS             complement(69718..70437)
                     /locus_tag="SCK51_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEG1250990.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            70774..71106
                     /locus_tag="SCK51_000338"
     CDS             70774..71106
                     /locus_tag="SCK51_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1250991.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            71093..73096
                     /locus_tag="SCK51_000339"
     CDS             71093..73096
                     /locus_tag="SCK51_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEG1250992.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            73115..73585
                     /locus_tag="SCK51_000340"
     CDS             73115..73585
                     /locus_tag="SCK51_000340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEG1250993.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            73606..74190
                     /locus_tag="SCK51_000341"
     CDS             73606..74190
                     /locus_tag="SCK51_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEG1250994.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            74205..75191
                     /locus_tag="SCK51_000342"
     CDS             74205..75191
                     /locus_tag="SCK51_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEG1250995.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            75181..75492
                     /locus_tag="SCK51_000343"
     CDS             75181..75492
                     /locus_tag="SCK51_000343"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEG1250996.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            75514..77295
                     /locus_tag="SCK51_000344"
     CDS             75514..77295
                     /locus_tag="SCK51_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEG1250997.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            77288..78664
                     /locus_tag="SCK51_000345"
     CDS             77288..78664
                     /locus_tag="SCK51_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEG1250998.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            78671..79306
                     /locus_tag="SCK51_000346"
     CDS             78671..79306
                     /locus_tag="SCK51_000346"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEG1250999.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            79336..80691
                     /locus_tag="SCK51_000347"
     CDS             79336..80691
                     /locus_tag="SCK51_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HEG1251000.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(80883..82277)
                     /locus_tag="SCK51_000348"
     CDS             complement(80883..82277)
                     /locus_tag="SCK51_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEG1251001.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            82369..83340
                     /locus_tag="SCK51_000349"
     CDS             82369..83340
                     /locus_tag="SCK51_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEG1251002.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            83337..84119
                     /locus_tag="SCK51_000350"
     CDS             83337..84119
                     /locus_tag="SCK51_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEG1251003.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(84288..84659)
                     /locus_tag="SCK51_000351"
     CDS             complement(84288..84659)
                     /locus_tag="SCK51_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1251004.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            84793..85236
                     /locus_tag="SCK51_000352"
     CDS             84793..85236
                     /locus_tag="SCK51_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEG1251005.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(85279..86088)
                     /gene="yidA"
                     /locus_tag="SCK51_000353"
     CDS             complement(85279..86088)
                     /gene="yidA"
                     /locus_tag="SCK51_000353"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEG1251006.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(86107..87474)
                     /locus_tag="SCK51_000354"
     CDS             complement(86107..87474)
                     /locus_tag="SCK51_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1251007.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(87458..88288)
                     /locus_tag="SCK51_000355"
     CDS             complement(87458..88288)
                     /locus_tag="SCK51_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEG1251008.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            88451..88888
                     /locus_tag="SCK51_000356"
     CDS             88451..88888
                     /locus_tag="SCK51_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEG1251009.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            88964..89584
                     /locus_tag="SCK51_000357"
     CDS             88964..89584
                     /locus_tag="SCK51_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEG1251010.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        29552 a        15170 c        18471 g        26460 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
     3181 tggaggattg gttgtttgtt aacaaaagaa ccactttcct ttctcttttt atataaaaag
     3241 ctttctattt ataattaatg actattataa caaaaatgaa aagccaaaac gacaagtaaa
     3301 atattttttt atataaatat ttgtgttact aattccgtta atatacataa gtgaaagcgt
     3361 tttatataag aacttgaaaa gaaagaaggg atagacatga aaaaaagata caaattggtg
     3421 atagctttgc taaacattac cctattattc agcttaagtg catgtggaaa aactaacgaa
     3481 gaagtaacag caaacatcgc agatccaatg ataaaaaaag ccactacatt agaagtatat
     3541 gtcgcaaacg gcaaactgac aaactatgtt gaaaatgctg caaaaatcta taaccgcaaa
     3601 actggagcaa atattgactt aaaaattgtg aatgtagctt cgggaacagc aacagtacaa
     3661 atgatcaccc caaaattggt aacacacgaa gatatgccgg atattatctc catggcagac
     3721 acttcagcag caggtgtgct ggaaaaattt caagatgctt tttatagtgc tgatgaatat
     3781 ggattttatg acaaatatgg cgatcagttt tatgatcaaa aattgaatat tttgcgtcaa
     3841 caaacgagta atgaaacgat tattccttgg gctagtgatt ttaccccagc actttgttat
     3901 tatcaaccaa aaaaatttgc tgaaataggt gtagattttg aagaaatcag ttcttgggaa
     3961 gaatatatcc aggtggcaaa agcattaaaa gaaaaaactg gtagttttgg aattgccttg
     4021 ccagaatctg gggaccaaga attgtttatc aacatgctag cccagcagaa tcaatctttg
     4081 cttgatgaag aaggaaatat ccgtctgaat actaaagaag cgaaacatgc tgcaaagtta
     4141 atcaaagaaa tgatcgactc agatattgta catttctacg gtgcacaaga tgctgagaaa
     4201 gcatttcaag aatctgcaat gtttgttgct ggcggctggt atgctacgaa tatgtctttg
     4261 aattttcctg atgcatctga caaatggcgc atggctccac tgattccttt ttcaaaagaa
     4321 aatccaggaa aaggtgcagt atcaggagga tcaagttttt atgtccctaa agatgccaaa
     4381 aatccacaag tggcacaaca atttcttaca tttatgctga cagatgaaga atgcttagcc
     4441 aacgcactgg agctgggcgt ggctacatct aactcattgg cctatcaaac agaagcagct
     4501 gagaaaaagt ttgactacta aggaggacaa aaatactatg aactattgaa taaaatcaat
     4561 gaaaatacat caaatactgt tttaccagcc acttattcag atgctacgtc ttatttgact
     4621 accgctgcat ataactattg gcaaacaggt gattttgaaa attcatatct aaaagaagca
     4681 gaaaattttg ctcataagta cggcgtaaaa ttaactggtg attaaaaaat attaggagga
     4741 cttaacgatg atgcaatctt cgttaccaag caagacaata aagaacagca agaaaaaagc
     4801 aacagatacg tctcataaaa aaagtgctta tggattggtc acaccttttg ttattttctt
     4861 tattgtgacg attttgattc ccatcattat ctcgtttgct tttagtttta tgaaaattgg
     4921 ctactcattt gaatttgttg gatttgacaa ttatctagct atcttccgag atccattgtt
     4981 tattaaatct tacacgaatg ttttgattat catggcagga tcaattccaa taacgatgct
     5041 attagctatt tactttgctg ttttattgaa ttcagcacag ttaaaaggaa aaggattttt
     5101 tagaactgtc tattatattc caaccattac atcaacggtg gctgtagctt ccgtattcat
     5161 gacttttttc aatccgactg ggttattcaa tggatttttg aatgcgctag gaattaacgg
     5221 tgtacagtgg ttaacaaatc ctttttggat acgaatatca atgatcatca cgatgatttg
     5281 gcttagtgta ggatataaca ctattttatt tttagctggt cttcaaggga tttctaaaga
     5341 gatatacgaa agtgctgaaa tagatggagc atcgaaattt cggcaattct ttttattaac
     5401 gatccccatg ctaaagccaa tcattttaat ggccacagtt ttagccacta tcaatggctt
     5461 gggtaatttc aatatcccaa atatattctt tgggacttcc aatggaccag aaaattcagc
     5521 tttggtcgta ggagtcaatt tatataaaac aagttttgaa atggttgatt ttggaaaagc
     5581 ctctgctatt gcctggacga tggtgtttgt atctgttata ttatcggcta ttcaatttaa
     5641 attcggaggt gataaaaatg actaaacaaa agatacaaag cagaattttt acgtacgtca
     5701 ttattgtgtt tggtgcctta atatttttga tgccatattt atatatgatt gtagcttcaa
     5761 cgcaaagtaa tcaagaaatt ttaagtggcg gaatcaattt caagtttggg gatatgctat
     5821 taaaaaactg gcaagccatc atggcgagat ataattatcc cttgattatt tggaattcga
     5881 ttgttattgc tgtaatcagt acagttttag ctacagccag ttctacaatg gcgggttatg
     5941 cattagcaaa gtatcgtttt atggggaata aactgttgtt caatacagtt atgctatcta
     6001 ggatggtgcc tttttttacc acattgattc ctttatttta cattatgagt cgtttaggat
     6061 tatcgaatac ttatgcaggt gtgatcatcc cttcgattgc cagtacaact tcagtgttta
     6121 tgatgcgaca atatagcagt caatttccta cagcactgat ggaggctgcc agaatcgatg
     6181 gtgccaatga gtgggtgatt tttacgaaaa tcgctgtacc gattttatca cctacgatta
     6241 ttactagtgg tttacttatc tttatgggaa gttggaatca atatctattt ccattagtca
     6301 tgctttcaga taaagataaa tatacggttc ccttaattat caaaaatatg acggtttcta
     6361 gtacaggtga gccaattaat tatggcgcaa ttatgctaat tttagcaact tcagtgatcc
     6421 caatggtctt gatttatgtg tgggcgcagt ctaaatttaa ggagaatgac attggagctg
     6481 caaataagta aaaaaaataa cgaatgttaa ccatctagtt cgttctagga gcaactccac
     6541 attcataaaa attaaaagaa tggcgggata tccatgaatg aacagacgaa ggaatggtca
     6601 agaaaaattt tgaatgtagc ttctgaaaag atagatgcgg aattagacag aatggggaat
     6661 aaaattccat atatttctta caacggaaaa tatacagacg taataaagga cagaggaatc
     6721 gactggtgga ccaatggttt ttggagtggt attttatggc agctctttca caagacaaaa
     6781 cgagaaaagt tcaaagaggc agcaatacaa caagaacaat tattagaatc tgccctgtac
     6841 catttttcag acgttcatca tgatgtaggt tttttattct taccatcggc tattgcacat
     6901 tatcaacagt ctcaaaacaa acatagtcga caactagggt tacatgcagc tacggtactt
     6961 gctggacgct ataatatttc agggaactat ttagtagcat ggaatgacca taaacctggt
     7021 tggatgatta ttgatagtat gatgaattta ccattactgt attgggcttc gagggaaact
     7081 ggtgatccga ggttctctaa aattgctcgg cagcatgcag atacaacagc taagtatcta
     7141 gtgaggaaag atggatcaac cgggcatatc gcagaatttg atgcagaatc agggaaatat
     7201 attcggttac ttggcgggca aggatacaat gagaattctt gttggagcag aggaaatgca
     7261 tgggctttat atggattcgc gttaagttat cgttacacaa aaaatccgta ctatctagct
     7321 atagcagaaa agatagcagt aaattttatt cagaaaatcg aaaaaactgg ttatattcct
     7381 ctcattgatt ttgctgtaaa agattcgaat attgatacca gtgctggagt atgcgctgca
     7441 tgcggtcttc tggaattagc aggatatgtt tcctctgtac gatcaaaaat gtatcatgaa
     7501 gctgctatca atatactcca aaagacagaa aagcactatg caaattggaa tatggatgaa
     7561 gatgggatca ttggaggcgg agcagaagca taccatagag aactgactta taatgtgcca
     7621 ctgatctatt ctgattattt ttttattgaa ggcttacttc gattagaaca gtcggaatta
     7681 gctatttggt agtaaaaaga ggagaaaata caatgaaaca tccagtacca ctattaagga
     7741 ccagacagga ttactttgac ttatttaaaa agataaatca accactccag ccgttttatc
     7801 gagcaagtgg cgcacaaatt gattatatac atcaaggggt aggatatgga aataaaattg
     7861 caggaatgga aggattctct cgggttttat ggggagcggg gcccgcaatt gaccaattag
     7921 atgaacgatg gttgaaatta attctatcag gaataaaagc tggaacagat ccttcccatc
     7981 ccgattattg gggagaaata catgaaagag accagcgtat ggtagaaatg cccgcaatgt
     8041 tattagcgtt atatcatcat gaacaattat tatggaaaaa attagataca ggagaaaaaa
     8101 gccagattat tcattggttc tcgcaaattt ttgaatatga atgtgcagat ggtaattggc
     8161 aattttttaa gataattgtc ggaacaatta tccaaaaatt tggttatcca gttaaagaat
     8221 cagcactgaa agaagcattt gataatatag aaaattgtta tcttcaaaat ggctggtatc
     8281 gagacagttc aagaggtcgc caagattatt ataatccttt tgcatttcat tattatggtt
     8341 tgatttatag cgtactagaa ccagaaaagc ccattagcca gatatataag aaccgagcta
     8401 ttgatttttc tagagactat attcattttt ttgcggaaga tggtgcaaat gtgccttttg
     8461 gtcgaagcat gatttatcgt tttgcagttt caagcttttg gtcagctatg ttgtgggccg
     8521 atcttttgcc ttttaaatta ggtgaaatca aggggttagt caatcggaat ataagatggt
     8581 ggatgcaaaa agaaatattt gatgaaaaag gtatcctgaa tattggttat acctaccctc
     8641 aattgacatt gactgaaccc tataatagta cgttatctcc attatggctg aataagattt
     8701 ttttattgtt ggcgctacct gaagaccatg agtattggac ggctaaagaa atgcctatgc
     8761 ctattcgtga aggaactaaa cttctttcag aagccaattt gctcatacaa catgataatg
     8821 gacatacatt ttttttgaat gcgggacaat tagggccaaa tttccataca ttgaccaatg
     8881 aaaaatattt aaaatttgct tattctagtc aatttggttt ttcgataccg cgagcgaatc
     8941 aattgaaagc agaaatggca atggattcaa tgctagggat tcagcgagcg gacacatata
     9001 tcacgaccag ttttaagaat gagagcatca aaacctatgg acagttcatc gttcgaaata
     9061 atgtacgaga cattgtatgt aagaaagagg tacttgcctc tacatgggaa cctactttac
     9121 aggcaaaaat cagaacttgg cttattcctt ttgaaggctg gcagattcgc gtacacaaag
     9181 tagaattaga tacagaatat gttctatatg agacagggtt tgctattgaa tgttctcctg
     9241 agaaagaagg aataatcata gaagaagaca aggaaaacta tagggtgagt gaagctggct
     9301 tttcaggtat tatctgttta agtgacgata ctattaaacg aaaatccaca gctattatgg
     9361 gtttgcccaa tacaaacttg atgacatggg aaaacacttt tattcctgga ttagagggta
     9421 catttggaaa aggaacccat tggctcggaa caggagtggt tgctcatcaa gatcgggatt
     9481 atagtttaga agtatggaac aatcgtccca aaattgattt tgatgggaca accgggctaa
     9541 ctatccaaaa taaaaacaat aaaaaaagac taaaattatg ctaaaggctg gtggggaaat
     9601 gcttgataaa gtgatgtttg aagagataat acatccagaa aaatggataa atgaatgtta
     9661 taaagaatta atggaaaaag aatggaataa tgagatcgag cgttgtcgca gacacacgga
     9721 gaggttaatc aaacgtatca gtaacagtca gtatcaattc ccaagcgagt gtcctataaa
     9781 aggacgttat ccattaagag acaatattgg ttggacagaa ggattttgga caggaattaa
     9841 ttggttgtta tttgagaaga cgggtgatcc tttatttcaa caaacagctg aaaaagcaga
     9901 tatagatttt atccacagga ttgactgtca aattatcact aatcatcacg atcttggttt
     9961 tttgtattct ttatcaaccg tagcagatta taaaataacg ggaaatcgaa aaatgaagga
    10021 agcttcaatt gatgctgcag aaacattact tcagcgtttt tgggagaagg caggagttat
    10081 acaagcttgg ggagatccta gtgatccttc acaacaaggt agaatgatca tggattgttg
    10141 catgaatctt ccattattgt attgggcgag tgaagaaact ggaaatcaaa aatataaaaa
    10201 agctgctcag gctcatattc aaaatgcagc agattattta gtaagagaag atggttcaac
    10261 ttatcataca ttttatatgg atgtcaaaga tggaaaacca agatatggaa gtacgcatca
    10321 aggttatagt gataattcct gttgggctag aggccaagca tgggggattt acggttttgc
    10381 attaagttat cgttatttga aagaacaaaa atattttgat ttaagcaaaa tattagccaa
    10441 ttattttatg aatcggttgc cagaagatac catttgttat tgggatttga tctttgatga
    10501 ttctagcaga gaagaaaaag atacatctag tatggtgatc atggtttgtg gattattaga
    10561 attaatagaa tcaggtctat tagacaaaac agaaaagaac ttataccagt ctgctgtttg
    10621 gaaaattatg cgacaattga aagaaagata tgctacaacg gaagaagaag atggcttttt
    10681 aacacagtct gtttataata agcccaaaaa taatggggta aatacacctt gtatttgggg
    10741 tgattactat tatttagaag ctttagtgag aatgactagt aattggtctt cttattggtg
    10801 aaaaaggtaa atatggaatt tttgtttgat aaaataaaaa aagaaaaacc aaaacagcat
    10861 gaatggtttc tgttttggtt ttttcgttaa ttttcatatt cttctaattc caagcttagt
    10921 tcttcccatt ccgtcaattt tgtttcttga tcttgtctag cagcttctaa atcttggttc
    10981 agctcattga gttttacgtg atcttccagg ttttctggcg cgaccatttc ttgttccaac
    11041 tgatcgatcc attcatcgat ccgcgccatt tcttcttcaa cctgagtaat ttttcgttga
    11101 aggctacgga taagcttttg ctgttctttg ctttggtaat aatcagattt gttcgtggga
    11161 gcaggagcag attctggctc atcttgtgcc aacaattcgg cgatttcttc ttcttcctgc
    11221 tttttctcta aataataatc atagtctcct agatagactt tgctgccatt ttcagagagt
    11281 tcgacgactt ttgtcgcgat ccgattgatg aaatatcggt catgggaaac aaaaagcagt
    11341 gtgccgtcat aatcgatcaa tgcattttct aatacttctt tgttgtcaat atctaaatgg
    11401 tttgttggtt catccaaaat caaaaaattt tctttgttca tcgctaactt ggctaaagca
    11461 acccgtgctt tttcaccgcc acttagcaga gggattggtt tttctacatc ttcgccagaa
    11521 aataagaaag cacctaaagc acttcggatc tctttttctg gagtggtagg atgctcatcc
    11581 cacaattctg ctaggatcgt cttgtttgta tgcagatcag cttgaccttg atcgtaatag
    11641 ccgatggtga catttgtccc tagcgtttct tttccacgga taaaagggat ctggtgaatg
    11701 attgatttca gcaacgtcga tttcccgata ccattcggac ctactagagc aatcgcttct
    11761 tgacgacgga tatctagatt gattggttca gataaggtga cattatcata tccgatagcg
    11821 gcatcttcga tttgcaagac tacatttcca gaaggctttt ctatactgaa tagaaagttt
    11881 gcttgttttt catccccttg aggacgatcc agccggtcca ttttttctaa tgtctttctg
    11941 cggctttgcg ctcgttttgt ggtagaggcg cgaacaaggt tccgagcaac gaagtcttct
    12001 aatttatgga tttctgtttg ctgcttttca taagccttcc attcacttgc taactgttct
    12061 gctttcaaat ctagatattt cgaatagtta cctttgtaat ggcgcatttt tcttctgctt
    12121 aattcataga cttcattgac tactttatct aaaaaataac ggtcatggga aacaatcaat
    12181 aatgcaccag tatagctttg cagataattc tcaagccagg aaagtgtctc gatatctaaa
    12241 tggttcgttg gttcatctag aatcaaaatg tcagggtgtt gcaaaagcat gcgagctaaa
    12301 gctaaacgtg tgcgctgtcc gcctgataaa gtatcgattt tctgatcata aaatgaagaa
    12361 tcgaatttaa acccatgcaa tacggaacga atttcatttt cataaccata gccgtttcga
    12421 tctgaaaaat cgtgttgcag ccggtcgtat tcttttaaat aagaactata agcaggtgtg
    12481 tctggcgtac cttcgctaat gaaaatctct agctcgcgca tccgctcttc catttgtcta
    12541 acttgttcaa aagctttcag catctcgttc caaactgttt cattggaatc aagtcctgtg
    12601 tcctgagcga ggtatccgag agtcgctgtt ttatttttag cgatctttcc ttcatcaggt
    12661 gcttcaattc cagcaatgat ctttaataaa gtcgattttc cagcgccatt tctgccaact
    12721 aaagcgacac gagcgcctgt actgatttct aaatgtatgt tttcaaataa gacttctgca
    12781 ccaaaatggc gagcaatctg attggcttgt aataatatca tggaatcctc ttttctactt
    12841 tgtgaattct cctcaaataa aaaactattt ttcaattagt agtctaccat aaattccatt
    12901 atactgcgag atttgcagaa atgataaata aatgaataaa gaataaaatt tcacaatatc
    12961 tgagcaatgg aattgatttt ttcctataaa tactgctatg atggagaaat caaagatgta
    13021 attagacaat acgtggaggt catttcgtga aagatcatat tattccaaag gcgacagcta
    13081 aaagactgcc tttgtattat cgctatttaa ggattttaaa taatgctgga aagaaaaaag
    13141 tgtcttcaac tgaattaagt gaagccgttc aagtagatag tgcaacgatc cgtcgagatt
    13201 tttcttattt tggcgaatta ggaaaacgtg ggtatggtta tgatgtagaa gatcttatgc
    13261 acttttttgc aaagacatta aacgacgatc aattgacaaa tgttgcgctg atcggtgtag
    13321 ggaacttggg aagtgcttta ctaaaataca aattccatca aagtaatagc atacgtatca
    13381 gttgtgcttt tgatgtgaat gaggaaatcg ttgggcgcat cgtagacggt atccccgttt
    13441 atccaatgga agatatgtca gaacaaatca agatccaaca aatcgaagta gcaattttaa
    13501 cgatacccgc tgaaagagca caagaagtag tcaatcagtt aacggaagca ggaatcaaag
    13561 gaatcttgaa ctttaccgct gctcgattga atgctccgtc agatgtattg atccaaaatg
    13621 ttgatttgac gaatgaattg caaaccttga tctatttctt gcattctgac gcaaatgcgc
    13681 attccgaaaa tacagcagaa taaaaataaa aagcgattga acaaatgaag gcaaaacttt
    13741 cacttgttca atcgcttttt tctgtttgga caacgattat tttgttcgga agatttcttc
    13801 ataagaaagg gaatcatctt ctgctgtcag ttcatctagt agataacgga tttttttaaa
    13861 gtcgatgaac cgtgcttgcc gaaagacttc ttttccttga tgaaaaatca ggacaactgg
    13921 cgcagtcagc acttcaaatt tactagctac ttctggtgtt tcaaatgcat ccaaatgaag
    13981 tgccggaata tcataatgtg tgagaagttc ctctagtcgt ggacgaacag cgtgacaaac
    14041 actgcaatta ggcatcgaaa gataaatgac ggcaacggat gactgttgca gtatgtcttg
    14101 aacttcagtc aagttagtgg tttgtttcat ttttattctc ccttttatga tagaagtgat
    14161 atgtatgaga ccttaattta atgggaataa cagaaaaagt caattgaagt gctttggtaa
    14221 aaaataagag ttgtgtaaaa atttacagag caacttgttt tttttgaata aaagcgtata
    14281 tttgcaaagc gaatgaacta gaaataaaaa ggaggaaact catgttttct tttattccaa
    14341 attcggtttt gcagatgagt acgattttct tatcgatcgt tattgaagca ttacctttcg
    14401 tattattagg ctgtcttatc tcaggcgcct tacaagtatt tttgacccca gaacgtgtga
    14461 agagatggct tcctgaaaat cgtatcttct ctatttttac tggttcgatc ttaggatttt
    14521 tctttccttc atgtgaatgc ggcatcgtac cgatcgttca tcaatttgtt aaaaaaggcg
    14581 taccagttca cacagcgttt gcttttatgc tgactgctcc gatcatcaat ccaatcgtga
    14641 ttttttccac ttttatcgca tttggcaatt cttggcagat ggcaagctgg cgggtgatag
    14701 gcagcttttt tgttgccctt gtgattggtg tctggcttgc ctattttcaa aaagacgctg
    14761 tattgaaaga aaaaatcacg tctaaagtag cagaaaatca ccatcatcat gtccatgaag
    14821 aagaacaaag tgagaagaat gaaccaaaaa atcagctgat ccatcaaact gggcatgtgt
    14881 tgacacattc aatcgacgaa ttttttgata caggtcgtta tttgatcatt ggcggattaa
    14941 tcgcagctgc gatgcagact tatttaccaa ctagaattat gctaacgctc ggctcaacaa
    15001 agattttagc cattatcatc atgctgttat tggcctttac gatgtctctt tgctcagaag
    15061 cagatgcgtt tataggatct tctttgttaa gtctgttcgg cacagcacct gtagctgcct
    15121 ttttggtctt tggcccaatg gtcgatatca aaaatctact gatgatgaaa cgatatttta
    15181 caggacgctt tattttatcg ctgatcggac tgattgccct tagcgttatt ggattcacat
    15241 tatttattta ggagggaaaa tatgctgcgg tttttgattt tatctgggta tttcgaattg
    15301 atgatgtact tgcaagtttc tggcaaactc gatcaatata tcaatgtcca ttatcgctac
    15361 ttagctattc tttcaatgat tttatctgct cttttggctt tagttcagct gtatttgtgg
    15421 gtcaaaaacg gaaaagcaga agaaaaggca cacgagcacg atcatgacca tgggttgtca
    15481 aaaccttatc aacgtggaat cgcttatgta ctgctagctt tgccagtaat cgtggggacg
    15541 ttgtttccaa cagttagtct cgacactacg atcgtagaag ccaaaggatt taatttccca
    15601 gtaagtaaag aatcagtagg ggatccggag atgcagacac aatacttaaa gccggatacc
    15661 agtatttatt tcaacaaatc agactactta gaccaaatga atgagttgaa agaaaagtat
    15721 ggggataagc aaactatcaa gatcacagat gaaaactatt tagaagttat ggagctaatt
    15781 tataattacc ctagcgaatt cattggcaaa aaaatcagct atgaaggatt tgtttaccaa
    15841 acaccgaacg atagcaatac agatatcttc ttgttccgat tcggcattat ccattgtgtg
    15901 gcagattcag gagtctttgg tttgttgacg catatgccgg aaggagtaac ggttaaaaat
    15961 gatgaatggt ataaaataga aggaaccatc caatcagact attatcaacc attcaagcgt
    16021 gaaatcccat ctgtagttgt tacaaacgct gagaaagtct ctgctccgaa aaatcagtat
    16081 gtgtatcgtt ctttttaaaa gaacaaaata aataaacata gctcagacag ctgacacgca
    16141 aggacgtcgg ttgtctggct ttttttgcat gatgtattca tttgtttgat atttatttta
    16201 gaagcagcat cctgaaagta tttgatatcg gtttcaaagg tgttggaaaa gattgtgaag
    16261 agaattacaa tttattccta acatgtatgt tgaaaagttg tatgaaatga ttttgatagt
    16321 aaaatatcga gtggaggtac tttttatggt aacgcgtata tttattttgg atgatagttt
    16381 ggtttttgaa aaaatgctag tagaaatttt ggaagaatcc agaattttat taagtccttg
    16441 gaaatttgag atcgttcagg gattgaacgt tatacaattt gttgaattca ttaaaaataa
    16501 tgaaatctat tcctcagata tttttttcct agacatcgat ttgaacgcct attatagcgg
    16561 tatcgaacta ggcgaaatgc ttagagaaaa gaatgaaaag tgtaaaattg tttatctaac
    16621 gtctcatccg aataaagcaa taactgttat taatcgaaag attacgccta gtggttattt
    16681 gataaaagaa ttcacttatg aaaaaaataa aaaaagcatc caaagttact tattgaaaga
    16741 aaaagaaaac tatcctgttc aacatgctac gaaaaaaagg tggacaactg tccgttatgg
    16801 taatagcgat cactatatcc attacgatga aatcctttat ctaatgagca tgccaggtta
    16861 taaaaataaa ataggcctag tattgaaaga tgaagagatt ctcgtaaacg gcacaattaa
    16921 aaaatataaa aaatcactcg aatatgagta tttatgtaaa gatttaaaag cttatatcat
    16981 taatactgaa aacattacat ctattagcag agtaaacgaa atagtaacat ttgcaaatgg
    17041 agaacaacta gagatgggaa agagaagtat tgataaacta aagaatttta tgcaagaaaa
    17101 gatagaaaat gaatcatttt aaagaaaaca aagactcata tcttacctgt tagaagagtg
    17161 ttttcctgta gaagagaaca acccaagtag cagcctagtc gtttctatcc ttttttgtta
    17221 tgcactcttt ttgacattta ctccaccaat aacgaagggt acaataaaaa gtaataacaa
    17281 acaaaaggag tacagaaatg attgaattca tcaatgtaag taaaagttac gggaaaaaac
    17341 aagcattaaa agacttgtct ctctcgataa gacaagggga aatttttgga tttttaggac
    17401 ataatggtgc cggaaaatca actacgatta aaagtttagt tagtattatt gaaccatcat
    17461 cgggaacgat tttagtagat ggaatgaaat tgacggaaaa tcgattgagt atcaaacaaa
    17521 aaatcggtta tgtgcctgat tccccagata tttttttaca attgacggct ggagaatatt
    17581 gggacttgat ttcagctgcc tatgaattag acacacaaaa aaaagaaaag cgtttagcag
    17641 aattgacagc gctttttgat atgtatagtc atcaaaatga aacaatcgca agtttttcac
    17701 atggaatgcg tcaaaaaacg attttgatcg gcacgttgct gccagatcca gatatttggg
    17761 tattggatga acctttgcaa ggacttgatc ctcaagcagc atttgatttg aaagaaatga
    17821 tgaaggctca tgccgcaaaa ggaaaaacag tcattttttc tacacatgta ttggataccg
    17881 cacaacagtt atgcgataaa ctggctattt tgaagaaagg tgaattgatt tatcaggggg
    17941 cagttagtga attattgaat ggctcacctg atgaaacgct agaaaagatt tatttgaaaa
    18001 tggctggaag gcaagcgaca ggagatggac cgtatgtata agcgacaatt attagaatta
    18061 attcgagtta atcttttata tgttaatcca cagctcacga ataaagcaag agagaaagga
    18121 aaaaagggaa agcgattaac tatctatctt ttgtctcaaa atcttttttc agggctgatt
    18181 tttttactga tttacggatt tgctatgttt gctatgaact ttgttaaatt gccaggtttc
    18241 tttacttatt acgtggcact ttttggtatg ttaggtttaa ctcaaagtat atcagtcatt
    18301 tataatatct tttttgaagg aaatgacctg caagtatttt tgcctctgcc ttttggacaa
    18361 gaacaggttt tttcagcaaa gattttggta gttgctttga ccatcattcc ttttgttttt
    18421 ccgcttctag tcatgtttat tttaaccgga tggcgagctg atctagggat tttgcttact
    18481 atcattatct caatcctttt gttttctcta tttttgatta ttcttttttg tctggctagt
    18541 ttgattgttt ttggtttggc tcgaacagca gtgtttaaaa aacatagaaa aacagtttct
    18601 agtatcttgc ttggcagctc gatgctgatc gcatttgctg gaatcatgtg gatgaatcat
    18661 caaacgggga ctattgaaaa tgagttaaca gatcggcatc cgatttcgat attattgcca
    18721 ttctatgatg ctgccagtca gccactttct caacaaggtt tattgggttg gggaatacta
    18781 gctatcacgg ctctgggatt tttccttgct gtaaaacagc tgatagtacc aaagcttaca
    18841 gagcaactga ctgattcaaa cagtgagttc aaaataaaaa gaacccataa aaagaaccaa
    18901 aatgtgcatc agcttttatt tagttacaat gctcaattag taaaagaacc taatttgatt
    18961 atgcaagttc tttcttcttc cttgttaact ccaatcattt ttattcttgc ttttgcccta
    19021 ggaggcgaaa taaaattaac tgatttagat aataagttaa tcggtgttgt tttcctggta
    19081 gggatcgcat tggctttctt aacagttaat cagacttctt ttatcagcaa tcttatttct
    19141 ttggatcaag aaaattttct gtttatccgt tctttgccaa tctctatgaa ccaatattta
    19201 aaagaaaagt tcagattcgg atggatattg cagagttttt taactggtgg gatcgcatta
    19261 ttaagcggac tgtcttttca gctgcctttg ttttttatcc ctagcttgct atttggttct
    19321 ttattcggct gctacttatt gtcactacgt tatttcgcta gagattatcg gttgctgctg
    19381 ctgaactgga ccaatattaa ccaattattt aacagaggtt caggaagttt gggtttagtc
    19441 gcaactatga tgggttcatt gatcatcagt attattctta tcgtcttata cggaatgttg
    19501 gctttgtttt atccattttg gttagtgaat attcctgtga tcggtttagt tctaatactt
    19561 tccgctctat ggatcgttta ttatcaacga acgttttgga aaaaatttga ataaagaaga
    19621 ggggccgtga caaaaatcat gtcgcagccc cttttttaca gatagagggt gttcaaaagt
    19681 cagtttctca gtattaagtc aattttatgt aaaaatgtga gaagctattt ttacaaaact
    19741 ttttttaata ctcataatta cttcttattg ctcctgcggt tacttgtcgc aatgtttgtg
    19801 agatattttg tcatcgctca aggctgaacg tctctttcac aaccttttct ttgttacact
    19861 tcgtttattt aaaaagccgt ttttttcgtt tcatcatgat tgcgtcacgc tttactttga
    19921 aattacggat gttatggtta aagacttctg ataatacaag acctaaagcg attgctccag
    19981 caatcataat tgcttggacc gcataatgag ccgcttctcg ataggattca gtaacgagat
    20041 ttcgaaccgc ttgataagct aaaccaccag ggactagagg aaccatcccg ggaatattga
    20101 aaatcgttac gggcattttc aaacgtttag agaaaagatc gctgatgaat gctactccta
    20161 aagaaccaag caatgaacca aaagcagcag atccgcctat ttgaacacag atccagtaga
    20221 tcatccagcc aaaagcacca gaaagaccac aacaaatcaa tgaacgccta ggtacatttg
    20281 taataacggc aaatgccgca gtcgctaaaa aactaaaaga aaactgaata aatatcgtcc
    20341 ccatacttgt gttcctttcc taataaaaaa gctggaaaat aaaagcaatc gcaaaaccga
    20401 tcatcgtagc agtcatcatc gcttctgttc cgcgagaaac tcctgagaca taatgtccag
    20461 ctagcaaatc acggatagcg ttcgtaattt gaactccagg aactaaaggc atcacacatc
    20521 cgataatgat tagatcctga ttgcttccta agccaaaacg tacgctgtag atagcggcta
    20581 tgccaatcaa taaagaagat aaaaattccg ataaaaattt gattctaaaa aatttcaaac
    20641 tatatagata agctgcgtat cccaatcctc caatgactaa agtcacaggt aaatcggtca
    20701 tctttccgcc aaataacacc ataattgttc cactgatcac tgccgcgcta acgatttgca
    20761 accagacagg aaagaaagaa cggtctcggt cgattgcttg cagttcgcag taaagttctt
    20821 ctaatgttag ctctgaagca acatatttac gtgaaagatc atttactttt actacttttt
    20881 ctaaattaat cgaacggttg gtaatttgtt ccattcggat ggtcgaggta cgatcaagac
    20941 caataaagag accggtttga gtgacatagc tgaccaaacg gaaatttcca gaagctaaag
    21001 caattcgact catcgtatct tctacccgat acatctccgc attgctttcc atcatgattt
    21061 tcccagcgag taaacaagtg tccagcaaca gatctaattc ttttcccgtc atagcatctt
    21121 ctccaataat tgattgatta gttttatttt actacaaaac ataccagaag cgtaaagaat
    21181 aaaaaaactt gaggaaggaa taaaagaggc gtatactgtt tttgtattgc gggtgtagtt
    21241 tagtggtaaa atcccagctt cccaagctga tgtcgcgggt tcgattcccg tcacccgctt
    21301 aaaataggtt gtgaaaaagc tgctctgcaa caagtaataa gaacagccaa gcaaaaacaa
    21361 cttcttatgt ttttaacttg gatggactta ttaccgcaga tgcaagcttt tgcgcaccgt
    21421 ttaccaagcg gttgtgaaat cagtgtattg tcttgagtat gaagaaaatt tttgaataat
    21481 aataaatacc ttacagatag agtggtgact gattagagac gaggaatccg tctctttttt
    21541 gtatttttaa gccatcttaa aacaggtttt attttttgct gtttctattt ctttctagta
    21601 tttatttata ggggaacata tgttaagatt tagggagctg ttagtaaagg agtaaagaaa
    21661 tgagcaaaaa aacaaccaag gcgatcgtgg ggatcgtcgc tgctgtgatt gcaggggcat
    21721 tcggtgtttc caccacacaa aaagattcag atatctttca gcagattaca actatttttg
    21781 attccacacc acaagcttct caagtagcgg atgtgccaga atatgatgga aaacatcaag
    21841 aaatagaaat caatgggaat cagccggaat ttacaaaaga ggatcttagc ctttccaaag
    21901 gaacatggga aagctattca aatattgata aattgaacag agtaggacaa gccaatgcta
    21961 tgcttggaaa agatttgttc cctactgaaa agagagagcc tttgtatatt gacccgacag
    22021 gttggaaaca aaagaaatta agtgacgggc aatggttata taatcgcagc catttaattg
    22081 gtttccagct gactggtcaa aataacaaca ttaaaaactt gatgacaggg acacgttcgt
    22141 tgaatgctcc atacatgctt gctcacgaaa atgatattgc ggcatatatc aaagaaacaa
    22201 atcatcatgt acgctaccgt gtcactcctc attttgaggg gaatgaactg gtagccaggg
    22261 gagtgcaatt ggaagcagaa agcattgaag acaaaaaaat tgaattcaat gtctttatct
    22321 acaatgttca agatggctac acgatcaatt atgagacagg acaagcaaca aaaacaaaaa
    22381 attagtagca gctattcgaa ttttttatga atattatgaa aaaattgtgt aaaaacatac
    22441 attcaaagaa atatagcgta tactttatga aaagagtact tttgataaag ctacagaaaa
    22501 aaggaaaaaa acatacaaat agtgtttctt aatttattcg gaagtttgga atatttgttt
    22561 gtagttttcc ctttcttcga gtaaaataag gttgtagaag gtaagctgga attaccttgc
    22621 gggaaagagg tgtagaatca tgggttttac agatgaaacc gtacgttttg gttttgacga
    22681 tagtcgcaaa aaagaagtga gcgaaacatt agcgattgtt tatcgggctt tggaagaaaa
    22741 aggctacaat ccaatcaatc agattgtcgg ctacttgctt tctggagacc cggcctacat
    22801 tcctcgttat cgtgatgctc gaaatttgat tcgccgtcac gaacgcgatg agatcatgga
    22861 agtaattgtc aaggactacc tctcaaatca tggagtgaat ttatgagagt aatgggcttg
    22921 gatgtcggtt ctaagacagt aggtgttgct atcagtgacc cctttggctg gacagctcaa
    22981 ggagtagaga tcattcggat caatgaagca gaagaagagt ttggctttga tcggttgaag
    23041 gaattagtag cacaatatga agtagaccgg tttgtagttg gattacctaa aaatatgaac
    23101 aattccatcg gaccaagggc agaagcttcc atgtcatacg gagaaaagat caaagaacta
    23161 ttctccttgc cagttgatta tcaagatgaa cgattaacca ctgttcaggc agaacgtatg
    23221 ctagtagagc aggcaaatac ttcaagagcc aaacgaaaaa aagtcatcga taaattagca
    23281 gctgtgatga tcttacaaaa ttatttagac ggctcaggta agcaattatt ttaataataa
    23341 aagaaaagag gtaatcttat gacagaacat aaccattccc acgaccatga aggacacgaa
    23401 cacatcacat tggtggatga ccaaggaaat gaaacattat atgaaatcct actaacagtt
    23461 gacggacaag aagaatttgg acgtaactat gttcttttat atcctgctgg tgtttcagaa
    23521 gatgaagatg tagaattaca agcttatgca tacatcgaaa acgaagatgg aacagaaggc
    23581 gagttagaac aaatcgaaac agacgcagaa tgggacatga ttgaagaagt attcaataca
    23641 ttcatggcgg aagaagaaga atagactata aagctaggag aaaccttgat atgatagggt
    23701 ttctcctttt tttatttttt actgtatgcc atattgtatg ccatctagtc aatatttaag
    23761 tgttgtacta atttttcaac agtcttttct ttcgtttctt ttgccaagtg accgtaacaa
    23821 tcgatcgtct gactaatttt tgtatgccgt aatctagttt gaacttctac catagtagct
    23881 ccagcgtcca agagcattga agcatgagtg tgtcttgcta catgcacacc gagaaatgga
    23941 accatagctt tttcacaaat agtaatcaat cttgcataaa tagcgtggtg tgtgattagt
    24001 tctttataga taccacaaaa aatcatagtc ggatcagtta caccaagtgt cagcatatat
    24061 ttccgctgat taatctgcca tttttttaat atagaaattg ttctttcatc tagtggaaga
    24121 accccagcac tacaatcagt tttaggatca ctgattttat agccattttt ggattggctt
    24181 agtgttttat tgatggtgac cgtattttct tgaaaatcaa tgtctgacca attcaatgca
    24241 agggcttcac tgatcctaac cccgctataa aagagaaagc gtagtaacgt tttatcaaag
    24301 tcgtttcgat aagtttcagg tttgctgttt aggtaatcaa acaaggtgat cagttgttct
    24361 ttactgtaaa ctttgacctt ttctttatgc ttagtggtgg ttcttttagg cattaatacg
    24421 ttgtctagtg gattagtctc aataatccca agattgatag cgtagctaaa aatacgctta
    24481 gaagttgaat gaagtacctt gtattggtca gtagtttcag cccagctatt tacaatttgt
    24541 tgggcttttt ttaccgtgac ttcattcaca cgaagatttc catattttgg aagaatccac
    24601 tttctaaact tgatttcagt tttcatgaag gtggcttctc ggacagtggt tttatatgtt
    24661 tctagccaca agtcataaac ttctttaaac gtttcgttgt tcgctttttt aataccgttt
    24721 ttatcgtagt ccacttgcaa gcgtgacaag gcaagctggg cttctttttt tgttttgtaa
    24781 cctcgtctac gaaccttatc aggtttgcca gtcattttgt tcattccaag ataggcttca
    24841 tagagccaaa atttaccttt tttagtttca tattgcttga atgttgccat aatagttttc
    24901 cctccatagt gtagcaagcc gtgggggcgg ttggaggagg cagtttatta ttattttgaa
    24961 tagtgtttat ttagctcagt aacaagagct gtgaactctt ttagaagatc attttcataa
    25021 aattcttgat actctttttt ctcatctgta tttttttctg aatcattaat taatgagtca
    25081 tatcgtaaaa gcgtatttag aagagctgcg agataagcaa ttggggtact cccgtcagga
    25141 aattcaattc gagatcctgt tttaagatat tttattgcgt ttgttaaata aatcttctgt
    25201 gagacagtca ggttatcttc ttcgatgtta ttgatagcat tagaaagatt agaatcaatt
    25261 atagagttat ttttttcaat agtttttttc aattgctttt caagcacatt tcgcttaatt
    25321 tcgggcatca tatgcatatc tcttaatgta taagatccgt ttaatagata ggtagtggat
    25381 acatctaata cctttgaaag ttgtttaagc ctggtgttat tgggaagatt atagccattt
    25441 tcccaattac taacggcacc tttactggcc ggtggggata atttacctcc aagttgttcc
    25501 attgagagtc ccttttcaat tctagccagt ctaattcttt gtcctgtctc cactgcttcg
    25561 gggtattttt ttctagccac attttcacca ccattcatat attttctcta ctatatcacg
    25621 atttaaatat gtgtgaaaca taaaagttca aaaaagtatg ttaaaattct tgcaaatgaa
    25681 aaggtatgat ggtatattga gtttataaga ggtatagaaa tgtattcaag agcctcttgc
    25741 atttttttaa aatctttgta tagaaatgta tgctaacgag gggggataaa tatggaatta
    25801 aaaattgaca aactcacgct tgatgatcca gcttttaaaa ttattgacga tcgagttgtt
    25861 caacaagtag agcaaactca aggaacgtta ctttcattat tagaaaaaaa gtaccagttt
    25921 ccaaaatata tgaacaaggc acaagctgct aagtatatga acgtttcata caatactatg
    25981 atgcagaaat atgttcctaa cggtttaaag ttgattatag ttgatggagt cgtaagaatt
    26041 tcacaagatg aatgtgatcg ctttatggaa gaataccaaa aataaacgca agccgtgggg
    26101 gcaaagggat taataggagg aaataataat ggaatcgaca caaaataaag caatcgaaaa
    26161 agtattatca acaattctta cagaagacac agcggcagaa ttagcaaact tggaaggcaa
    26221 agcgctagaa gaaacgtttg aatgtttgta cgaacaaatg gactatcaaa aattgctacc
    26281 acaagaacca acggtaagcg gagtattgcg tggcttgaac gatttagtac aagcagagtt
    26341 taaagaacga ctttctattg aagagtatca agaaattttg tatcagcaag ttgagttgtt
    26401 agcgagcttg ctagggcttg aactggagga cgttgaatga aaactgaaat ttggaacgga
    26461 cacattataa gatttgtcga tattaatgat gaatggtggg cagtggcgaa agatgttgcg
    26521 gaagcgttag gtcttaaaca ggttactaga gccattcact ctttgcctaa agatggggtt
    26581 actactagta aggtcatcga ttcattgggc agaacacaag atgtaaacat catcaacgaa
    26641 aaaaaatatt taccgcatgg cattcaaaag ccgtaaaaaa gaagccgagg catttcaaga
    26701 ctggattttc gacatcatca aagaactacg ccagtccaca ggacttgaag gctttcaagt
    26761 attccgcatg ctagacaaag aacaccaaaa agaagcaatg actaaattaa gccacgctat
    26821 tactgaacca aaaccagtag actacattaa agcgaatgtg atagccaata aagctgtatc
    26881 aactatttat ggtcattcca aaatggtaaa aaagaaagat atgactcctg aaatgttggt
    26941 tgatcgtgaa cctattttag atgagacagt agaattaatg actgttaaag aaaaatatgg
    27001 gttgcagttt tcagtgagtg agaaaattta taatcgttct gctgaattgc agacaactta
    27061 ggagcattaa catgatcgag aacaaagaaa aagccttatc cataacagtc ttggcggaca
    27121 ggataaaggc aacttaattg ggataaaacc cgatattctt tgttccgatt ataccatatt
    27181 aaaaaatcgg agggaaccat tatgaaacga aacttaatta taggaagcat tttaatcggc
    27241 ggaattttcg gcttattacc attaattgca aaaatagcta tctttgcagg attagcgtta
    27301 tatctcttca ctgcttatga cgagtacgac tatcaattaa gaactggagg gcataagtag
    27361 atgtacaaaa caaaactatt aaaccaactc gatagccttg aattagagga aattaatcaa
    27421 gggatcgctg aactagaaaa taatatcggg aaaacctact ttggaaattc attcaatgaa
    27481 aaactaacag ttttgtatgt actcaaaaaa catgcagaac acaaaataat ttgtcgagaa
    27541 atcaacgagc taaaaaatca gattttaacc gcatggttaa acattactga tatgcaagaa
    27601 gcaagggtga aaacatttaa tacatgggta aagtatcaga atcaattaaa aggtgctgaa
    27661 tttgttcggg atgggttgaa atacgaactt gagcagttga agctcatgga ggtgtctgaa
    27721 tgatttatac cagtattttg aaaaaggagg agaggtttat tggcaaataa ttatcaagca
    27781 gcaacggaat tacaaaagtc gggatggtca atttatccct tatctcctgg aactaaaatt
    27841 ccaataaaag gctctagtgg ttggaatgat gcaactaacg attctaatca aattaatgaa
    27901 tggtggacgg aagatgcaac tagaaatatt gggttaatgc ttgaagatca tcaaatggtg
    27961 gtagttgacc tagatcaaca ttctgaaaca cgaaacggcg ttgagaatta taaaaaactt
    28021 gcttcaatgt atgatccatt tccttctact tatacggaaa tcacaccagg taaaggaatc
    28081 catttcttct ttaaaattcc tgatggtgta attatccaac aagaaacagg tgcatttgct
    28141 gatatcttcg ggcgtgatga taaaggtaaa agtttaagcg ggattgatat tatcactaaa
    28201 ggagttccaa tagcgccaac tatgattatt tcaagaagtg ttgggagagc atatgaacct
    28261 attgaaaacg tatctctaaa taatattgca ccagctcccg attggctagt gaacctactg
    28321 ctaaaaaaac cgcaagaaat aaagactcaa taccgacccg tagcaaaaac agggacagca
    28381 aaaaaattag atatgattgt tcaaggcgct ggcgaaggta gtagaaacga tcatttaact
    28441 aagttatgcg gttggttact atggcacggt gtagataatc aaactttagc agaattgatt
    28501 tatacagcaa atgcgtacaa tgaaccaccg ctaaaagata aagaagtgaa tcaaattatt
    28561 cgatcgatga ttaaaaaaga tttgaggggg aaagctcatg gctcataagc ataaattaga
    28621 tagtatttta gactttcccg aagccagcga gcgtgaagac aatatcattg aattgaaaac
    28681 ctggatgtca cgattaagat gcaataaaga cgatcagatt aaaagtaaca gtgttgtgaa
    28741 tgcagaattg attttaacca atgatagcaa tttagctgga acaattgctt ataacgaatt
    28801 tagcggatac attcacctat taaaagattc tccttggatc aatcgtaccg ctggagaatg
    28861 ggaagacagt tttgaggatg ccttaacggc atatattgaa gaaaactaca atgtagtttt
    28921 tgatgacaac aaaatacaca aagcagtagt aaatgtggct agaaagaatg ttttcaaccc
    28981 agtaaaagaa cgtatcgaaa aagttaaatg ggatcaacag cctagactgg aaacactgtt
    29041 tattgacctt ttaggggtag acgacaacct ttacacacga gaagtaacaa aacgttggat
    29101 agtcggatca gtcgcacgta tctataaacc aggaataaag tttgagatcg ttccagtttt
    29161 ggatggaccg caagggattg gtaagagtac cgttccagca ttactttaca cggatgattt
    29221 tttcaccgat tcattagatt cgctaggtga aaagaaagat gattatatgc agcttcaagg
    29281 caatgtaatt attgaacttg gagaattatc ttcaatgaac aaaactaaaa ttgaacagat
    29341 aaaaaacttc atcagtgcca agatcgataa aattagacct ccttatggtc ggaatgtcat
    29401 agcttgggca aggcaatgtg tatttattgg aacgtcgaat gatgggcagt atttgaaaga
    29461 tgatacagga aaccgccgtt tttatccttt gccttgtaag aataagccaa agatgaatcc
    29521 gtttaaaaca aaggatgaat attttcttca agtgttagct gaagcaaagg tgctttttga
    29581 taaaggacaa cgaatctatt ttagtcccga tgaagataaa gaagtcttag aaattgcaaa
    29641 agactatcaa gaggatgcca agatggagaa tccaattaaa gaagcaatca ctaaatattt
    29701 ggaaatggaa atcccgtata aatgggaaga agctccagcg tgggcgagac gttcttatta
    29761 tcagaactat cctgacagta aatgtaatga gaaaaattta cagcatttct ataatggagc
    29821 tactatgcaa caagagtttt atttagttga tagcgtactc accgctgata ttttggaagc
    29881 tgtttttgat aaacaagcaa aggatttatt gaatggtcga tcagatgcag aaactaaaaa
    29941 gattgccttg attattagca gtattcctgg ttgggaacgg aagcaactgc caaaaagaaa
    30001 taaacgacga ggattttaca ataagtcaaa tgcctatgaa aataaaaatc gagctggaaa
    30061 gtacaaaaag taacgggtag taacgggtca gtaacaccta gcccgttact agtttaccct
    30121 tactgcccca agggatacag caatttggta acggggtaac accttatttc cttaaaactt
    30181 ttgaaatagt tagctaatat gctggggaat aatgttactg gattttaccc gttacctgtt
    30241 accaaaacgc tagaaccctt gtccccgttg ggattgagtg gtaacgggta cctgttacgt
    30301 aggtgttacc acgtgttacg aatcaaggag gaaagtaaat gctcaatatt atttcaacaa
    30361 acaaagcacc gaactttcaa tatacagatg aaatggatcg gtttctaatg aacacgctgg
    30421 catttagtgt gggattggta acagaagatt attccacatt tgatccagaa gtattaaaga
    30481 taatggaaga agaacctgat tggctgcagg agtcagtagc gtggtgtcaa tcgctagtag
    30541 tgggatcatt ggtagatagt ggcaactatg atgatacagg tgagctaatg gatgaattta
    30601 attgtctgct taacctatat gatcgagcga gacaacgaga gcttacatcg aatgaggata
    30661 atttgttttt aaacatccat gataagttct tggctttgct actaacagat gatgaattga
    30721 taactaattt attggaggta gaataattat gcatatggaa agtattagat acttcgatgg
    30781 tgagaaacac attgagtatt caaacactca agaagatgta gactttatta gttttgagga
    30841 agataaaatc actaaagtta attttaaaga cggaacgtgc ttgaagatag tttcgcctta
    30901 tattgaatac aaatcaaaat gggaacaaga tgattctgac gaacgagatc gctttggttg
    30961 gtaatctatg gcagttaaaa aacaatgtaa tcatgctgga tgtaaaacat tgattgatta
    31021 taggcagaag tattgtggga agcataaagc aaagcaaacg gcaattaagc gtgaagaaag
    31081 aaagcaaagc gaaggtaaat attttcaatt ctatcaaagt agaacatggc gaaaggcttc
    31141 atacttgtat cggttaaatc atccagtatg tgaggattgc ctagaagaag gcttgataag
    31201 gaaagctgat gttgtagatc acaaaattga actaaaagat gattggaata gacggttaga
    31261 tgaaactaat tttcgttctt tatgccatgc acaccataat tccaagacag ccaatgaaag
    31321 acaaagacga gaaaagagta ccctttgagt taagggggca tagtgtgaaa cttactgaca
    31381 atcgatgcct actcattttg gtacaaataa ccgttgtgaa aaggcataag ggtaattaca
    31441 aataacgatt acacttgtaa tcacaagtga aaaagtatat aatgaaagta ggagatttat
    31501 tgaaaaatac aattgttatt actgataaaa cagggaaaca gcgcaccatt gagttacctc
    31561 attttggtca agtgaccatt caaatgcaga acggaaaaat catttatata gataaattag
    31621 ataaagaaaa attctgatcg aaaaacgaag gaatgtgagc ttaattgctt acgttccttt
    31681 ttcttttgtc tgaaaggagg acaacatggg acaccctaaa atattacacg atacaaaagg
    31741 caatatatca agtgaagaaa aagcaatccg tgtggacgct agagaagaat tgttcaagca
    31801 gcaaccatta ataaatatca cgccccctga ctggatggca gcgagcgcta gaagtgaatg
    31861 gaatcgtatc gtaccaacat taaaaaaaga ttatccattg agtgaagcag attatggttc
    31921 attggtagcc tattgtttag cctttgctcg aatgaaaaca gccgaagccg agataagaaa
    31981 atcaggtacg tttatcacat gcgaaaacgg agtaaagaaa gccaatccag cggttcgggt
    32041 tcaatcacaa gctatgagtg atttgaaaaa acaagccacc tcactaggta tgaccttaga
    32101 atcacgatca aaactagctt tgaacaaggt taaaaatgat gaacccgaag acccgttcaa
    32161 agagttgatg aattcatgaa tgattacatt gagaaagtcc tatcaggtaa gctgattgca
    32221 cccaagaaaa ttatccaagc gtgtgagcgt catataagcg atttggagcg ttctaagtca
    32281 gatagcttcc cttatgtgtt cgatgaagaa caagccacca aagcgattaa attcattgag
    32341 ttgctaccat ctacggacgg taaagcaatc aagatgttag gatttcaaaa attcattcta
    32401 ggcagtcttt atggctggcg tactaaagaa gggaattaca ggcgattcaa tcgagcgttt
    32461 accagtatga gccgtaagaa tgggaaaacg tatatcgcaa gtggcatggc tgccaatgcg
    32521 ttgattatgg aacaagaacc agcagaagca aggcaagtat tgtttgtaag taacgctttg
    32581 aaacaagcta aattgggcta tgatatgctg tctaattcac ttagaaacgt ggtcaagtct
    32641 agtaagtttt taagaccaca actgaaaatt atgaactcta agattcagca cttgccctct
    32701 aattcgttcg ctatggcact ggctagtgaa accagcacgc tagatgggtt tgcaccaaca
    32761 accgcaattc ttgacgagtg gcacgaagca aaaactcgta aaacgtacaa cgtcattaag
    32821 tcaggaatga cccaacaaaa gaacggctta ttgtgtgtta ttagtaccgc tgggcttgat
    32881 ttaaacgttc ctatgtacga ggaatactta ttgttagatc gtgtgctaaa aggcgaagaa
    32941 caagccgaca ggtactttat agcgatatgg gaattggacg atcccgaaga aattcacgat
    33001 caagagaaat ggatcaaagc caatccgatt tttgaaagtg aagaaatcaa aaaagtaatg
    33061 attccaacca ttcaagatga tgtgaacctt gctttgaaac aaaataacct taattctgta
    33121 ttggtgaaaa acttcaattt atggagacaa gcgagtgagg acagctatat gattgctgaa
    33181 gactggcagg caaccgaagt agaaccacaa gatattacag gcaagcccgt ttatatcgga
    33241 gtggatttat ctaaaacaga tgatttaact agcgtttcat ggatcgtacc gctagataac
    33301 ggcgaacttt attgtgattc acatagcttt gtagccacca aatatgggct tcaagacaaa
    33361 gaaaagcgcg acggtttgcc ttatcgagaa ctagaaaaag ctggtgagtg ttccattact
    33421 caattagaaa gtggaatcgt ggactatgac caagtatttc agtttattca agatttgatt
    33481 caagaaaatg atttggaatg tatggggatt tgttatgacc cgtataacgc taattcgctt
    33541 atcagtaaag ctgaaaaagc caactaccca atgttagaag taagacaagg aacgattact
    33601 ctaaacgttc cgacccgaac ttttagagaa caagtttatg aaggcaacgt tattcacaat
    33661 aaaaatacga ttctcaccca tgcagtgaac aacgctattt taaaaacgga taacaacggc
    33721 attcagatta ataaatcaaa gaacagtaac aaaattgatc caatagctgc attaatcaat
    33781 gcctatgtgt ttgcaatgga ttacttcacc acaacggaag gagcgaaagc agacaatgaa
    33841 ttttatacaa gtgaagaatt ttctttctaa ttacattcat accgttcttt tacttcttgg
    33901 attggtgtgt gtgttggttg ctatcacctt actaacaaat gtctactatg gcttgttagc
    33961 gctgggcata gtgcttattg gggtagcggt catgctaaat acagaacaga agggaggtta
    34021 aaagatggca ttttttaaag cgagacaaaa tacaacggga gatcctttcc tacaacatgt
    34081 ggtatcaatc caatcggatg attacaccac cagctttaca agcgttcgtg cattaagaaa
    34141 tagtgatgtg tttgcagccg ttcgaattat tgctagtgat attgcttcaa gtccgattca
    34201 attggtcaaa aacaatatgc cacaagctga taatgaactg gtgaagttac taaacgagaa
    34261 acctaattca gaaatggatg gttggcattt aaagtttgct ttggcggtca atatgttgct
    34321 aaacggtaat agctttgcag agattaaacg caacggtgaa aaggtagaag aaattcactt
    34381 attacctaac tcaagtgtaa cggttactca attagataat ggtacgttgt cttatcagat
    34441 tggtgataaa aaaagacgtg tgaagtctag cgatattttg cacttcaaat atttcaccca
    34501 agatggttta acaggattac caccacttta tgcgttacgt gatgaactga aaattcaaca
    34561 agctggcaac cgcacattgc acaatttctt tactcgtggt gtcagtgggt caggtatttt
    34621 gaaggttcat aagtcagatt tagacggatc agcaaagaac gcaattcgtg aaaagtttga
    34681 agaagccaac ggttcaagta gtggagataa tgctcttaga acgatcattc ttgatgaaac
    34741 aatggactac aaaacattag aaataaacac cgatgttttg aagcttatca attccaatga
    34801 ttggaacaca aaacaaattg ccaaggcgtt tggtgtaccg attgagcgtt taggcgttga
    34861 aaatgaacat tctagcaccg ttcaaagtaa tctccagtat atccaaagta cgctgattca
    34921 ttactttaat gtgtttgtga gtgaattaga tacgaaactt gaaaccaata tccgctttaa
    34981 ttccgatcag ttactagaga ctgatcctga aacaaaagta aaaaatgtat tggatcaggt
    35041 caaagggtca cttctcacga ttaatgaggg gcggtcgaaa atggggctac cccccatgga
    35101 tggtggcgat cgtttactag caagtttgaa ctttacgtat ttagatacgt tggagaaata
    35161 tcaattaaaa gaacaggaag gagttacacc agttgaataa tgaagaagaa aaggaaaaac
    35221 ggctgacaga agaagctgag ctaacagccg attctcccaa agtagagaaa gaaaatgaaa
    35281 aacaaccaac agacggcaaa actatttcag gctatgcgtt gaaattcggg caaccgtcaa
    35341 aagatttagg cggctttgta gaagtgatta cacccgaagc attaaaagag gtggatttat
    35401 caaatgtgtt cttattatat ggacacgatt attcaaagcc actagctagt gtcaaagcag
    35461 gtacgttgaa attgaatgtc gatgatacag gattacattt tgaagcagag ctaaccgaca
    35521 cgacctattc aaatgatgtg tatgagaata tctcaaaagg ggttgttgat tctatgtcct
    35581 ttgggttcgt gcttggatta gattcattcg acaaaaaaga agatggcaca attgaacgat
    35641 caatagataa aatcaaagca cttaatgaaa ttagtgtcgt gaccgttccc gcttatgatt
    35701 catcaaatgt ccaagtaaat aagcgttctt acgaatcgtt tatgagtaac aaccaagcaa
    35761 aacaaacaaa caatagctta gaatccactt ctaaagcaca aaaggagagt aataacatgg
    35821 aaaaaacttt aatcgataac gaaaaaacag aattgcgtgg gtatgaagaa tatatccgtt
    35881 cacaaggcga agtgcgtgat ggagtcacta ctgtaaatgc agcggcagtt gttcccgaag
    35941 aagtaatcgg tgaagtcttt gatttgaaac gttcaaatta taacttagct caatatgcaa
    36001 cagtaaaaac agtatcaaat ggacaaggta aatatccagt agcaactaac caacaagcag
    36061 tgttagcaac aaaagctgaa cttgctgaaa ttggtgatat tgacgctgaa atgtttactt
    36121 cagttgatta taaagtagaa actcgtgctg gtaagattgc cttatcaaat gaggttgtgg
    36181 aagattcagc agtgaatatt gtacaagagg tcaaagatca gttagcgaaa ttggtagaaa
    36241 acactgacaa taagcatatc atggatttat taaaaacatt cactaagaaa acggctgcta
    36301 cgttggacga tttgaaacaa ctatacaatg tggcattaga cccagcatta aataaaatgg
    36361 taattctaaa ccaaagcgga tattaccacc tagatacatt gaaagattca gatggacgtt
    36421 acattttaca acccgatgtg acagcaccta gtggtaaatc attattcggt atgccagtag
    36481 tattgattgc agatacattg tttgccaatc ctaaagctgg aacattccct atgattatgg
    36541 gggatattgc acaatctatc tttgttgctc gtagaaatca agtaacgact caatgggaaa
    36601 aattcgatta ctactcacaa ggacttgcag tgatcgttcg caacgattat aagaaaattg
    36661 atcaaaatgc ttcagtgtat attgagttta cgccagttgt aacaccaaaa gtatagaaaa
    36721 atattgggcg gcggtttatc ccactgcctt tttttcatta ggagagataa aatggtaatt
    36781 ttggatagca taaaaaaaag tatgcggatc gatcacacta ttgatgatga ctttattcaa
    36841 caattgattg atacagcagg cgaatatata aaaagtgcta ttgatagtag cgcaacggat
    36901 aaagatatgg ataattatca gcaatttgat ttggcggtgt cattacttac tcaacattgg
    36961 tatttgaacc gtcaagaagc cagcagcgaa cggataccag taacggtaca agcattagta
    37021 caacaaatga gaggtgctta ttatgccaat cattaagaat gtaaatgaat tgactgagag
    37081 agtcaagttt aaaaagacaa aacgggtaaa agatgaagat gggcagatgg tggatagtga
    37141 agaaactgta tttgaatgtt gggctaatgt gcgttcacaa atgctaaaag acgtgcttgc
    37201 tagtgtaggt accattcttg aaggaacgtt gacgtttatt attcgatacg atcaagatta
    37261 tgaactaaca aacgatatga aagtagcttg gaaaaacaag agttataaaa ttatttcaat
    37321 caatgaagga acggcgttta aagattatac aacgatcata gctaaacaga tttcttagaa
    37381 tgattacact tgtaaacgtt gtgggtattt agtataatta aggtagtaaa tgaagggtta
    37441 gctgcctgaa ttttgcaaga cttagctagt cgaaatctat tgttggactg aaaattgtag
    37501 gtgtggttgc aaacatgctg gactaagaaa gatgatagat gatgaacgtt caattttaat
    37561 acaggcatgc taggactgat tggatggttt tggcaagctg attgtgtaga aatcaaagaa
    37621 cacgtctgtt gtggacgtgt tttttttgtt ataattcaaa agaggtgatt taatggacaa
    37681 agcagagttt acaaattttt attggaagta ttatctacat ttagaaaatg agtttattaa
    37741 taccacagat tttgtgatgc tcgatgagaa taatttcaaa acattctcta ttgaatatca
    37801 aaaactacta ctagcgattg gttcggagtg tgagattatt ttcaaagaac tttgcggatt
    37861 tgatagcagt agcaataaaa gcataactga ttttaaagct gttattaaat ctagcgaatt
    37921 aatgtgtttg gataatggtg ttagggttat gaattcttta acattgactt cactaaaacc
    37981 ttttggagat gggtggcctg aagatacacc agattggtgg aaaatatata acagagtaaa
    38041 acatggtcgt tcttcaaatt ataaggaagc aaatttggaa aatgtgttgt atgctttagc
    38101 aagtctatat ttattagagg aatatttgca taagaaagta atactagaag gtgaagtaga
    38161 ctttataagt ccagagtcaa atctatttac gttatcttgg gaaagaaaac attcgtcgat
    38221 gcaaaaagtg acgtttgaaa aagtggattc aaattatgta gctccactca ttgattttaa
    38281 agaacaagaa gaaatttaaa aacccccacg gcttattgaa gtttttgtat gccatattgt
    38341 atgccatttg gtttaaatcg cttgaaaaat agtgatagat aactctaatt catttagtca
    38401 taaacgctgt tttgataacc tttatagttc tttgatatgg gtttctcatt catggcggaa
    38461 gaagaagaat agaacaaaag aatgaattaa taagatataa aaaaagggat tgtgacaaac
    38521 gtcttgtcac aatccctttt tccaaataaa cgatgttcaa tcagctgccc ctcggcattc
    38581 gctcgaaaaa cggcgaaata tgaagaacta ttttgccatt tgtttctcta ataatcaggt
    38641 caaaacgttg acttcacagt ctcttcctta aacggtgttc tcctgccaac tcctcggtca
    38701 caagtcacaa tattcaacaa acatgagaag ctgttttttg aaattgttcc ttgtgactcg
    38761 gagctgaacg gcaggctcac gacctctttc ttaaacggtg ttcaaaagct tgcatctgcg
    38821 gtaataagtt caaccaaacg aaaattatga ggagctattt ttgtttggct gttcttatta
    38881 cttgatgcag gacagctttt tcacaacctc ttttcgttta gcactaaatt tccttaaaaa
    38941 gtagaaaagc tttcgaagcc tcgtttgttt cttttactgt ataataaaag aaaagagtaa
    39001 caatcgctaa aagatataaa caagagaaag gaaatacaag atgattggtc tgatttattt
    39061 tgtggtgatc gttctggcga acacagttgg cgctatttcc gggatgggtg gaggcgtgtt
    39121 gatcaaaccg attcttgatc ttatcggcgc ccattctgtt gcagggattt ctttttactc
    39181 aacagttgcg gtattcacga tgtccattgt ttcgactgtg cgacaagtat caagcgggaa
    39241 atcactgaat tggcagattg tcggttgggt atctagtgga gctgttgttg gaggagtagt
    39301 cggcaacatt gtattcgaag tttttttaca gttatttgaa aacgagaagc atgtgcagct
    39361 gatccaaatt tttttgacgg tattgacctt ggtttttgcg ttcttctaca ccaagcatca
    39421 tcagccaaaa tttcatttga cgtcatggac ttggtacctt atatgtgggg ggggttctgg
    39481 gctttttagc tagtttttta ggtattggtg gtggaccagt caatgtttct ttgttgatgc
    39541 tgatgtttgc attgccaata aaagaagcaa cactatattc tctatctacg attttctttt
    39601 ctcaactggc aaaattggta acgattgctt taacaagtag ctttatgcgc tttgatttat
    39661 ctatgttgtt ttatgttatc cccgctgctg ttattggagg tttgtgggga gcaagattta
    39721 gcagggtttt gtctccaaaa aaagtgacct ttatttttca agtaattgtc ctcgtggttc
    39781 ttttggtcaa ttgttacaat gcctttgtca tcttacaaac attaggagaa tctagactaa
    39841 cgcaagtcaa aatcactgaa acatattgtt ctaaatgaaa aaaagagtat actatgataa
    39901 aaaaattcat tttaaaaaat cagaaattgg ctggtgattt ggatgttacg ggaaatgcgt
    39961 ttagaagatg cagaagcact acgtgaaatc aatgcggatc aattagggta tgatatacca
    40021 ttaaccgtta cggtgagaca aatgaagaaa ctgctgaagg agccagagaa aaattttttc
    40081 ttggtttatg aagatcctac ttctaaaaaa gcagctggct atgtacaagc ggagtcctat
    40141 gaatcgattt ttagcgatcc aatgttcaat attatggcac ttgctgtgtc taaatatgct
    40201 gaaaaaagag gcatcggaaa ggctttgatg actggcgtag aagaagaagc gaaaaaacga
    40261 ggaataacga caataaggct taattctgca gaatacagag tagaagcaca ccaattttat
    40321 gaacacatcg gttatcattc cgataaaatg caaaaaagat ttttgaaaat cctgtaaatc
    40381 tttatcaaaa aaatacccga tcaccaagcc gatacttgat gatcaggtat ttttgttttt
    40441 ttgactaaga aagtaattgc atcaagaaac cagagatgat caaaacgatg gcaccaccaa
    40501 gtcgattgcc catttgagca aaagcaataa gttccatccg attagctgca gataagacgg
    40561 ctacatttcc tgttccgccc atcgagttgt tgcataagcc agcagtaata gttgattcaa
    40621 ttggatataa accaaacaaa cgaccgacaa atcctgaaac aactgaaatc gcgataacac
    40681 ttgtcagaca taagacaaca aattgccatg tcaaagcaga agctaataca ttcagattca
    40741 gcaacgcaat accgataccg gcaagaacag ctgctgtcaa gtttttgaca atcaagttat
    40801 tgaacatgat agctgcatct tcataatact ctggtaatag gttggcgatt ttacagatga
    40861 caacgataat aatcataaat gcgtaagcat ggactttagg aacaaaatag ttaccaattt
    40921 gtcctaagat gaagaaagtc aatgatacca tcaaaccaac acctaactgt ggaatggaag
    40981 gtttcacagt tcgaggtttt ccatcgccaa gatcgccttt ttccattaat ttaccattgc
    41041 cgttgaattt tggcattgat tgtccagcac gagcaactaa tgctgcaccg atgatagcta
    41101 agacatttgt cattgctgtc gcaggaatca ggcgggaaat gatagaagaa gcatccgtac
    41161 ctaaaacatt tgcataaatc gttgataaag gaacggctcc ggctccaaca ccaccagcca
    41221 tcgcaggtaa tgagatgtat aaaacggaat ttgcaaaacc atttccgata agcatgccca
    41281 cgagtcctac ggcaaaaaag gagacgacca ttgaaataag tgcaacggga ataaaacgaa
    41341 cagaagcttt catcaataga ttacggttca taccaaggat gctaccagta atcaaggcag
    41401 caatataaaa gtcccaaaag cccatattgc tgacaaagtt ttctgtggat ttcactacat
    41461 attcaggtaa gatcccaaat gtagcaatcg cagcagaagc gaagatcgca aaaacggaac
    41521 caccacccaa ataacttttg acgataggga tttttgtccc gataaaatga aataaattac
    41581 cgaaaataac caaaactgcg attggtccaa tcatattcgt cggtaatttc cctaaagcca
    41641 ttgcaatgat caggacaagg accatgatca aataaagtgg caaacttacg ccggtgattt
    41701 ttgtggacca aaaaccttct tttttctttt cctcaactgc agttgcagtt gttgtttttt
    41761 gtgccatttt tacagatcct ttcttattct gtatcatatg aaaaaagcca aagaaaagcg
    41821 acaaaagagc aggagttggc tggccgctgt tttgccgcag tcgctttttt ctttttagac
    41881 gtcatacaaa gtttctgtat gttcttttac tataacgatc ttaatcttct aaagatttat
    41941 attcaggaac ccatttagca gattcaactg cttctttgat atccgtgatt tcttcacgat
    42001 tcaagttttg tgcgacaact gattttgcga cagtttcagc aatcgtttga gaaaattcag
    42061 tgatttttgc tactggaggc aagatagctg ctcctggttt tgttacatca acgattccgc
    42121 ctaatgctcg actagcttga gaaatgattt cagcatttac tcgtgtagct gttgaagcaa
    42181 tcaaaccaag tcctaaacct gggtacatca aagcattatt tgcttgaccg atttggtaag
    42241 ttacaccatt atattcaaca tcagcagcag ggatacctgt tccaactaat gcttttccgt
    42301 ccgtccattc tattaaatct tttgcttttg cttctgccaa ttttgttgga ttagacaaag
    42361 gcatgatgat cggacgtggt gtatgagcag ccatttcttt cacgatttct tcagtgaatg
    42421 ctccaggttg ggtagaagta ccaatcatga ttgtcggatg gatcgctttt acaactgctt
    42481 ctaagtttgt caattcatca gcattagtga aatctgagcg tttgcgagcg aatgggattt
    42541 gaccaggagt caatccttct gtatcttcaa acaataaacc ttgtttatct acttgataga
    42601 aatgttggcg tgcttcttct tcaggtacac cttgacggat catctcatct aaaaggatgt
    42661 ttgcgatacc cacaccagca gtaccagcac caaatgtcaa aattatttga tctttcaatt
    42721 gttcaccgga tatattcaat ccgcctaata cacctgctag gacaacaatt cctgtacctt
    42781 ggatatcgtc gttgaaagta gtgatttgat cttcgtattt ttctaagatc gctgcagcat
    42841 ttcctcgtcc aaaatcttcc caatgcaata acaattctgg gaataattcg gtagctgatt
    42901 gaacgaattg atcgatgaat tcatagtaag tttcgccttc tacacgagca tgacgattgc
    42961 ctaagtataa gggattattc aataattctt cattattagt accagcgtcg atcgatacag
    43021 gcaaaacttg tgctggatta ataccagcag cagcagtata gaccatcaat ttgccgatag
    43081 cgatgtcaac accgttcaca ccccagtcgc ccatacctaa gattccttct gcatcagtca
    43141 caacaatcaa acgaatgtct cgtccatctg ccgcgttctt caaacttgct ttgatgtttt
    43201 ctggttcgtc gattgaaaga aatgcagcat cttgcggttt caagaaaatt tcgttgtatt
    43261 gttcgattga atccgcaacg acaggatcat aaacgacagg catgaattca actaaatgtt
    43321 cgcccatcaa tttataaaac aatgtccgat tcgtattgaa tagattcatt aagaagatac
    43381 gtttttccaa atctgtttgt ttgcttaagt attgaccata agcttgaaca gattgttgtt
    43441 ccagtgtttg gacagtactt ggaagcatac cagtgattcc atattttgcc cgttcttctt
    43501 ttgtaaaagc tgttccttta tttagaaaag gattatttaa taagtttaac cctgtaagca
    43561 tgaatgattc ctcctaagtt ttaatacaaa cgaagtatag aagtcagatg atgatatagt
    43621 caaaaagagc aggtatgata aattttattt atatgtaata tagccaaaag aaatcaaaat
    43681 agcagtattt gttcttttta tatcgacaat ccttgcttga atactatgcg tatctcttat
    43741 atgctagaga aagaaaagta gaagaataag ggatagatgg aatggataaa taggatttct
    43801 gaggaaaaaa gcacatgata gcgttgatag gagtcaccta tgtcaggtga taaaaataca
    43861 taagtagaaa attaagagga ggaagagaaa atgaaagaag ttcaattgat tagaaaaagc
    43921 gaactatcag aaggcggttg caatgcatgt ggcgtagtag aagcgacaag ctatacatta
    43981 aaattagatt ccaatcaagc aataatctct gaactgacag taggcggtct tgtggacagt
    44041 ctggcgcttg ctgaaggttt tactggagaa gatatttatg agatgttttc agaagtccgt
    44101 cagttaaaaa aaggagaaaa atgcatagag gttcatcatg agagcccgaa tgtgaggttc
    44161 aaacgtggtg ataatgagat gatcttcaag aatcatgtgt ctgatcacac tgaactatat
    44221 gaaatcgtga atcaaatact gtctgggatt tttgaattag gaccttatga gttcaaggaa
    44281 gaaaacggta atccaaaatt aaatgaagaa tggcaagaaa cgattgaaac acaaagaaac
    44341 aatccacact tatttcaata gtcttccaca gtatttcgta aaaatgttga tgaaaaagaa
    44401 acaccgtgaa tcagaggtcg tgaaaaacct gccctgcatc aagtaataag aacagccaaa
    44461 caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagttttt
    44521 gaacaccgtt taaggaagag actgtgaagt caacgttctg acctgagtat tagagagaca
    44581 aatggcaaaa tagttcctca tattttgcca ttttttgagc taataccgag ggtcagctga
    44641 ttgaacaccg ttttatcgag aggttgtgaa aaagtcgctt agtttcgagt agtaagaaaa
    44701 attttgtgaa aatggctttc cacatttttg cataaatttg acttaatacc gagaaaatgg
    44761 cttttgaaca ccgtttattc aaaaaaaggg actttgacac tacttttgtc aaagtcccat
    44821 cggtttttta tctattttta ttacaatcat ttcttccaga gtagtgacat ctggaatggc
    44881 tgaaatgtaa acagattatt tgcctgtatc aatgtcgaag agtaatataa aaagtgtaaa
    44941 cgctatcata ttgttcatca ttcaaatttt atagagagaa ggcattgaga tggaaaaaac
    45001 atatcaagca ggtacaaatg aaggaatagt agattttatc aatatggaag atttagaaat
    45061 cgcggcttca caagtgattc cagctggtgg ttatggttac atcagcagtg gtgctggcga
    45121 cttatttact tatcaagaaa atgaacgggc attcaatcat cgtttgatta ttcctcatgt
    45181 attaagagat gtagaattac ccgatacaac tacacatttt gacgaagaaa tgctgactgc
    45241 accaatcatc atggcaccag ttgctgctca tggattagct catgtgaagg cggagaaagc
    45301 ttctgcaaaa ggtgtagcag attttgggac gatctataca gcaagttctt acgcttcttg
    45361 tactttagaa gagataagag aagcaggcgg ggagaaagca ccgcaatggt ttcaatttta
    45421 tatgagcaaa gataatggaa tcaatttgga tattcttgaa gtggccaagc gtaatggagc
    45481 aaaagcaatc gtattgacag cggatgcaac agttggcgga aaccgtgaaa cagaccgcag
    45541 aaacgggttt accttcccgc tgccaatgcc gatcgttcag gcttatcaat caggagtagg
    45601 ccaaacgatg gatgctgtat ataaatcttc caaacaaaaa cttagtccaa aagatgtaga
    45661 atttatcgct gctcattctg atttgccagt ctacgtgaaa ggtgttcaat cagaagaaga
    45721 tgtctatcga tcattagaat ctggtgctgg tggaatttgg gtatctaacc atggtggccg
    45781 tcaattagat ggggggcctg ctgcgtttga ttcgttgcaa tatgtggcag aagcagtaga
    45841 taaacgtgta ccaatcgtgt ttgacagcgg tgtacgacgt ggacaacatg ttttcaaagc
    45901 aatcgcatca ggtgccgatc ttgttgctat tggcagaccc gttatttatg gattgtcttt
    45961 aggaggaagt acaggagtcc atcaagtgtt tgatttcttt aaaacagaat tggaaatggt
    46021 catgcaatta gctggaacac aaacagtaga agatatcaaa aaaataaaat tgcgtgagaa
    46081 tcgttttatt taagaaataa atgacgaaaa aaataggagt gcggcagatg atgccgtact
    46141 cctattttta tagttcaagg atgtcactaa tagaataatt tgaaacgaaa aaataaaatt
    46201 gaaatatttt ttcatactta ataaaaaagg aagaatattc atgtaaacga ttttattgtt
    46261 tttttctctt ttatacaaaa agggtcataa tttcaagtaa gcggatgaat tcatgaggat
    46321 tttttgcata ctgtctctac tatacttagg tcactaagaa aaaggagtga tttattatga
    46381 aaaagaacat gattgggaca ttattacttt caacacttct tctaggcggc ttggcaacac
    46441 cagcttttgc tgagggtcaa gcgacttcaa aaggagatat tacctttact gaaccaacaa
    46501 ataccgttga gcctcttaac cctactgatc cttcaaaacc agtagagcca gcagatccgg
    46561 aaaatccagc aactggccag acaggttctt taacattgga tgtagttcct gaattgccat
    46621 ttggtacaca cgagattgaa agcggaacaa aaacgtatca agtagacgct tcaaaaaacg
    46681 atacacctta tctccaagta tcagatcgtc gaggtgtagg tgcagatggc caagcacaag
    46741 ggtggaacgt gacagtttcg gtttcagatt ttgtaaacgg ttcacaagtt ttgcaaggcg
    46801 ctgaattaga ttttggaaca tccactgtta aaagtacgtc agataacgaa tccactggac
    46861 caactagtca aactgtaact gggttgagta aagcaagtgc agcgacacca atatttacag
    46921 cagcaaaaga tcaaggtctg ggaacatggc tttctgttta tgatccagca aatatcacac
    46981 tgaaagtgcc gaaagcagca gctggaacat tcacggctga tttgacttgg aacctcgtgg
    47041 ctggcccagt agcatagagt atgaaataaa ttcaacaaac tgcggtgaaa atactatagg
    47101 tgaaggattt tttaccgcag ttgttgttga aaggatgtgg tggacaatga atcaaagtaa
    47161 acgactgttt gtttcgtttt tttctatttt aagtctattt ttcatttttc cgtcaatcag
    47221 ccaagcaaag gattcagcag gagactttgg aatcaaaccg gtatttccag agaaccaaat
    47281 agataaagca atcggttatt ttgatttatt agtggcaccg gaacaaaatc agacactaga
    47341 agtaattatc agcaattctt cagatgaaga acgaacattt gaggtctctg tcaatccggc
    47401 ggtaacgagt gatggtggaa cgatcgatta tagtcaaaaa aacccaacgt tagatgagac
    47461 attgccgttt gatgtccgtg acgttttgct cattgcgaaa aaggaaatca atgtatcagc
    47521 acatgcagaa acaactatac ctatagaagt gaaaatccca gcaaaaagct ttaagggacg
    47581 tgtattagcg ggtattcatg tttcacctaa agaagaagct gaaacagaaa atgctacaga
    47641 aggagcacaa atcaaaaatc gtattgctta taatctcgca gtcgttttac aagaaagtca
    47701 agagacgata gaaccggatc tgaaactttt atcaggagat ttggacgagg tcaacgcaaa
    47761 gccaactgtt cagcttcgtt tccaaaatcc tcagcctagg attatcagca atttgatatt
    47821 tacgagcaag atcttttatg aaaatcagct ttatattgaa aatacgtcta atgaattttt
    47881 agttgcacca aattcgaatt ttcacttgaa tcttgattta gctggtgata aagcaaaagc
    47941 cggcgattac cgcgcagaaa tcatagcaaa atcaggcgac agtaatgaat ggcgatttac
    48001 acaaaacttt acgatcaaaa aagaaaaagc acaaaaagta aatgaaaatt ccgtttttgc
    48061 agtacaagaa caatctttcc catggcttta tgcaggattg gttgctgcag ttctagtgtt
    48121 atggttgatt ttgctgtttt tgtgggtcca caagaaaaag aaaaaaggag aaacaacatg
    48181 atgaggtgat ttcgttgaaa acatcgatct tattgggagg gattattgca agtttgctgt
    48241 tcttcagctg gcaagagcca gtccaagcag ataccaaaag ttcaggtacg attacatttt
    48301 atgatctaag tccagttccg catccaagtg gttcagtcaa tggcgcatca aaacagcaga
    48361 cacagctacc gatttataaa gaatctaatc aacatggcgt attcccggag acgaatgaag
    48421 aaaagatccg ttttctttat ttgatcattg gtatagaatt atggcttctt gcgtggttgt
    48481 tgttatggag aagaaaggaa aaggaggatg aacatgttgc tacaaaataa attttcattt
    48541 tttcgctcct tcttttttac ttcgcttttt gttttaagta tatttatctt ttcaccttcg
    48601 gcagaagctg ttggagcaac gcctcctgcc atttcttatg atccatacac gtatgaaaat
    48661 atcgtacctc caaatggtgg acgtagtgca gtcaatggcg ggaagcttgt ccaactaact
    48721 ggggctaacc aattgcctaa attgactgct acttatttga acgatggttc aaatgatcct
    48781 tggaaaggtg gcacggtccg ttttcattgg gaaagccatg tgaaactaac aaatgtactt
    48841 gatttggtga atatcactgc acaaggattt gcatttgtgg cgatcatcaa tttgccggaa
    48901 catgtatcaa gcagtgatgt attaaaagca attgactgga acaacgccta cttgtatatt
    48961 gccggtgccc ccatcaaggt tactagcggt agtttattat cagcaggaag ccataccttg
    49021 cgttttggga tggggtcgtg ggatacaaat actctattgg cagatattat tgctattatt
    49081 acgcgaggcg aattcaacgt ggacgatatc ccgcttcaat ttgatgttac agctaatgtc
    49141 gcgaagatga cagaaaacgg agataaatat gatacgagtc caaacaaaat tttgaccaag
    49201 ggtaaattac aaccttctat gtctaaaaag gccgtttttt ctgttgattt ttatgattcg
    49261 aataatatta tccaagatcg tatgttcaat tcgattttag ctccagctgc aggtagattt
    49321 tatcctaaat taactggcgg aaacttgaat ccaggagcat atgcattttc tgcaagtgca
    49381 gagataaata cgtggaatag ttacatctca acaagtgatt caacaggaaa atataataca
    49441 tttgctgata ccacagaata cgtggatgga aataaccaga accttccagg ctctaccaat
    49501 agtcggacat tgacgatggg gctgactgaa ggacgttttg atcagttgcc atttagtcgc
    49561 tttgatcgcg tagtcaacta ttttacagga aaaaacgtta cacaaggagc aatactgact
    49621 catactccag caagcacaag tggattgaat cagacagcca atatcgtgta cagtgggaaa
    49681 gatgcgcaag gcggattgct ttctcctgtt gctttaaagg tattggagca gtacaagcta
    49741 gacggaacgg ttttaccaac aaacttaaca gccgatagtc agtggggaaa gctcctttct
    49801 tctgcatcgt accaagtcac ggccaattgg tcagcaactg aactaaaaaa gggttcgatc
    49861 cattacaggc tttattcgaa aggaacccaa aaattagcga caggatggac agatcaactt
    49921 ttccaaacga tccaatcaaa taacgggggt gtaaatacag ctacttctac actacctgca
    49981 ttggcttcag ggcaatatta ttttgattat cgcttggtgg acgatgtact tactaacatg
    50041 tatccgtctt ttgcgtacaa atggcagtct gaacaagctg gaatcaccac tcttcctcaa
    50101 ataacagtgg ctaattttcc atctgtgagt gcagatagtc agctaaaaaa ccttagcaga
    50161 caacctgaga caggagatcc cctgtctgct ctatcaggag atacgattca agaaaatgtg
    50221 acattcacgt taacaaaatc tggggatgcg ataaccgaca aaaaaatcac gatctcactt
    50281 cctgaaaata ctacttatat caatggttcc ttaaaattaa atggtacaac tatcgcagat
    50341 accggcattc aacaaggagt ttctgtccca gcagatctat tgagcaaaat aggagatacc
    50401 attcatctta cttacaacta tcaattaaat actgtagata cttctgtaca atccgtcagc
    50461 atactgacaa aggcagctgt actttccagc aatattacat tagcagatgg tgctaagctt
    50521 cctaacccag ttgtccaaac atctgctaaa acaattcttg tccccaaaca agaattaaca
    50581 ttagttaatg taccagatga ttttacattt ggtaacgatc tccccaaacc gttgaagaca
    50641 agttattatg aagcaaaagg cgatttttct tttgatgtgc gggatactcg gcttccaagc
    50701 acgtctcctt ggcaactgac agggacattg acttctttat ttaagaatga tcaaggtcaa
    50761 gaattaagcg gaacaaaatt atacttcaat cattctggat caaaacaatt gattcagcaa
    50821 ggtcaaaaca cgttgattta tgagagtgat ggaacagcta aaggagaggt tctcgttgac
    50881 tttcccgata ccgatggttt actcttagag gtaaatagca gtacgaatgc tcaacctggc
    50941 gcaacgtatc aaggcatggt cacatgggaa ctgacagctg ggccaaccag ctaagtgatt
    51001 agaatttgta tggatctgtg ataactgtca cagatcctct ttttcaagga tgagaaggaa
    51061 acttctgctg ttaaaatcag aaatgcttgg aggagtggac ttggaacttt tcagtttgct
    51121 tgaaaaaaat gaacaacggc aaatcaaact tttccaactt ttattagagg aaaaaggaga
    51181 gatttccgtt aataacgtac ttacccgctt atcagttgat cgggctacct tgaaagaaga
    51241 tctgttttat ttacaagcta acttggaaaa tatcaaacaa ttggaaattg ttatccaaga
    51301 agggaatatt ttattaatca gacatggaaa tatagcagca gtcgatgttt attatcattg
    51361 ctacttgcgc tattctacaa aatatcagat acttctttat ctgttggaaa atggctcatt
    51421 tgatcggcag aaacttgtca tggaacttaa tatcagttcg gctactttgt ctcgcagagt
    51481 gaaggaatta aatgattctt taaatgagtt tcatctgaaa ataaaaagcg ggcggctgac
    51541 cggtccagaa agtcaaattt gttattttta tttccaatta ttttggtttg gacgtcctta
    51601 tcttgaaaac aaaaaagaat acagtgattt atcaggaaac acttttttag atctgctgca
    51661 aaaagagttt agttttacta ttacagaaga aggacgattg aaatttgaaa tatggctttc
    51721 cattatgaag aagcgaatgc gctgtacgaa aaaatccaaa ccagattata gcaatcttcc
    51781 tgttggattg tttcaaacga cacaattttt attggacctc catacatttt tgacccgtta
    51841 cttctttcaa tatgcctttc cttggaatca aaatgaaacg ttggtttttt atcttttcat
    51901 gatcagtaac tttacattag atacacataa ctcgcatgtc caggagtttt tgataagcga
    51961 aaaatacaaa gacgacgtag tagctcaatt gaatcgaacg tttaaacaat cgatagaaga
    52021 atatttctct ttttatgttt ttccagaaga gatttcgcaa aagattgcgt tgacagtgat
    52081 gcaaaatcac gctcattttg cctattttag aggatggatg aatatttttg gccaagaagg
    52141 attaaaacag cggttaactg aagtagaaga taaacaattc ctggttttat gtgaaagatt
    52201 ggtcagacaa acatttgcta ttgttgattt tactgaagta gacgaggaaa atgcccgctt
    52261 tgaattattt gctcgctatg catctatttt gcggacgctg atcaagcagt tagaattacg
    52321 tctgcaaatt gcttatgatc ttccttatga aaggctaatg gcggatatcg tgaaagaaat
    52381 catccaggaa caattgctga atacgatcaa atatgattta gtcgaatacg agaaagaaaa
    52441 gcagtatgat atcattttga ctagccaatt gaaagagtac ccgtcacaga agtccgctaa
    52501 agtttttgta ttcactagca acgaatataa ttatgatttc ccctatttga accaattttt
    52561 aaaagaatgt tatttagaaa aattgaattt aaggatgaat aaaacatgaa aatcgtgatc
    52621 attggtgctt cccatgcagg catcacagct gccctaaatc taagaaaact tcaaccgaaa
    52681 gcagaagttt tactgatcga tgaagatcat aaagatggac taggctatgt ttcaaatgga
    52741 atcaaccttt atttaaaagg aaagatacgt agtttagctg aagttgccca taacatgcgg
    52801 actcttcaag aaagtggggc aaaactgatc accgaatgga gagtaactga acttgatcca
    52861 gacaaacatc agctcatcct cgcatccaaa gaaggaaaaa acgagaccat aacctatgat
    52921 aaattaatcg tagcaacagg ttcctctcct gtcaccttgt ataaacaaat tgaagctgaa
    52981 aatgtctaca cctataaaaa tctagtgcaa tctaagcaag tacttgccgc tttaaaagaa
    53041 gcaaaagaag tagtgatctt cggtgctggt tatattggat tggaattagc agatgctttg
    53101 agaaataaag gatacattat tcatctagtt gattatatgc cgaatgtttt atcaagatat
    53161 ttcgataagg atatgatcaa cagttttcaa aatcagctgc aaactaagca aatcaatttt
    53221 tatccaaatg aattcttgat cgattggaaa aaatcagaag agaaagtagt atccgtacaa
    53281 cttctatctc aagcaataaa ggcagatatg gtcatttttt ctgcccaaac tcgtcctaat
    53341 acgacattgt taaaagaaaa agtggctttg tatgaggatg agactgtgat agtcaatgaa
    53401 tatctgcaga caagtgatcc ggacatctat gcgattggag atatcgtccc agtcagtttt
    53461 gacaaaaata aacggcatct ctttttacct ttagttactc gagctgtaca tatggcaaga
    53521 gcagtagcgc taactttgag tggtcagcca accgcttacg acctcagaca aaagataaca
    53581 gcagcagtca ttatagacta ctttttagga actgtagggc tgacagaaga tgaagcgcca
    53641 ttcttggaac aaaataccgg ttcgtgttca ggagaatttg atctatttcc acaatacgat
    53701 gaagaaaaca aaacagtaaa tgcaaagctg atctaccatc cggatacatt agaaataatt
    53761 ggcggacagc tgataagcca agaattctta ttatcagatt tgaacctgct tgcggatatc
    53821 gtgaaacaca aaactacgat tccacagcta gcagttgagg attttggatt tctgtctgaa
    53881 cacacctctc gttttcacta cctcaatgag ttagctttta aagtactagc taagagagca
    53941 aatagcaata gagtagataa aagactgtga gaaatatcac ggtcttttat tgtgtgggga
    54001 tataataaat attaatcaat cgagaataaa aaattcatat aatgaaatag cggaagaatt
    54061 ttttgagcat aatattcgcc aaaaatttat caagtgataa aattgttgta taaaatgatt
    54121 atttcaggag gaggagttaa atgtttttag cattaaatga aattatgcat tcaaagttac
    54181 gttatgcgtt agttgcaggg gtgatgtttt tgatcgccta cctggtattt tttttgactg
    54241 ggttagctta tggcttagct caagataatc gaacagcagt cgacaagtgg gaggcagatt
    54301 cgattgtgct ttcgaaagat gcaaattcaa atctagggat gtccatgatt actaagaaga
    54361 tagcagaaga agtcgaaggt gggaaagtgg cgtatcttgc ccagacacct ggagtcgtga
    54421 ctagtaaaga cagtacagaa gaaggaaaaa tcaatgttag cttcttcggt atcgataaaa
    54481 atcaattcat catgcccaat cttgttgaag gaaaagcttt tgataacgat gatgaggcag
    54541 taggcgatat tagtttgaaa gaagaatatg gtctagccgt aggagataca gttaagttat
    54601 ctggtagtga caagacgttt aaactaactg gctttacgga tcatgccaaa ttcaatgtct
    54661 caccagttct ctatacaacc atcaatgctt accaagagat ccgcttcgaa aaagaagata
    54721 caagtgaaaa tgcacggatt aacgcaatag ttgtcagagg taaaatcagt aatttacctg
    54781 aagatttaga acagatcaaa atttctaaat tcatcaatga attacctgga tataatgcgc
    54841 aagtattgac ttttggattt atgatcggat ttttgatcgt tattgcggct attgtgatcg
    54901 ggattttcat ttatgtactg acgatgcaaa agattaatat ctttggtgta atgaaagcac
    54961 aagggatcac aggaggattt attgcgcgtt ctgtagtggc acaaacattc attttatcat
    55021 ttgttggtat tcttcttggt ctattaggaa cagtgggaac atcccttgtt ctacctgatg
    55081 cggttccatt ccaaagcaat tggctgtttt ttggcgtcat tagcttgttg atgctggtag
    55141 tagctgtgtt aggcgcacta ttttctgttc ggacaatcgt aaaaatcgat ccgttgaagg
    55201 caattggata agaaggagga agagcatgaa agcaatagaa tttaaagaag tagaaaaaag
    55261 tttccaagat ggcgaccaag tagttaaagc attgaaagaa acaaccatga gtatcgataa
    55321 aggggaattt gttgcaatca tcggtccttc tggttcaggt aaaagtactt ttttgacttt
    55381 ggcaggcgga ttgcagacgc ctagcagtgg ctctgtattt atcaatgatc aagaattcag
    55441 taatcaaaaa gaaagtgaac gcgttaaact acgttttaaa gaaattggct ttattttgca
    55501 agcttccaat ctggttcctt ttctgacagt aaaaaagcag ttattattag tagataagat
    55561 caaacatgaa catcgtcaaa aagaagcaaa agaattattt gagcagcttg gcgtggataa
    55621 gttgatcaac aagtatccag aagaactatc tggtggagaa cggcaaaggg tcgcaatcgc
    55681 tcgtgcattg tacaatgatc cgtccattat tctagcagat gaaccaactg ctagtttgga
    55741 ttcagaaaag gcacgagaag tagtcgatat cttggcaaaa gaatcaaaag acaaaaataa
    55801 agcaacgatc atggttactc acgatacacg gctgattgac aaatgcgata gaatatttat
    55861 gatggaagat ggtgtattga aagaaaaaga ataaccaaaa agcaggtgga aaaatggtcc
    55921 atcgatacaa caaagataca cagcaaaaaa ttttgaaaga agcgactgac ttgtttatgt
    55981 ccaagggata tctcggcact tcaacaagag agatcgcaca aaaagttggg atcacccaac
    56041 ccaatctcta tcattatttc ggtgataaag aaaaactata tacggcagtt ttagaaaacc
    56101 acttaaaaga tgttggaaaa gctttgagag aaatcgttca aacaagcgaa acgggatttc
    56161 aagtaacctt gacaaaaatg gcgcagtttt tgattgagac acatctggtc gatttgtttc
    56221 tgatgcttca cgatcttgaa agcaatcttt caaaggaaac aagagatcat cttttctttc
    56281 tatggaagaa aaattatcgt gaaccttttg aagaaatctt ttcagcaaac caatctgtgc
    56341 taagaaatgg gatttcaaaa gaaatcgcag caaggcattt tttcttagtg ttagcacctt
    56401 acatcactcg gtcgaaaaga cctgtggaag aaccgctaaa tgtggcgcaa ttgatcgatt
    56461 tatatttgaa tggtgtatta ggagaagcgt aaagctgtta tatagggaga aaaatagtta
    56521 ggtgaagaag caagatatga cgaagatcgt tatatcttgt tttttttata taggtagttt
    56581 ttcataaata caaatttgtt attctctcat gattatcagg gtatattttt atttattatg
    56641 tcggtgttcc aggaagtgtt ttaatttaat aacgaagaaa ttaattgttt gtagaatgaa
    56701 ggaaactatt tattgggtag atgaatcacc acgaatagtc aaaaggagat ttcaggatat
    56761 gccaaaaatg aaaaaaatca ttttagcagt aatgttattt tctacggtta tatcttttac
    56821 agcccctttt ttgcgtactt aagctgattt taagttttga cccttagtct ggatctacga
    56881 ggtgagagtc aagaatgaaa aaaataattg gagcttttgt atttgcaatc attatcatta
    56941 ttatctgttt tcaaatagat gtttttggtc aaaaaaagat agttggacaa acggtcagca
    57001 gtacgaacag tacggttaca aagacaaagg aatcatttga tgatacagaa caagagacat
    57061 taatcaaaat cggaaaaaat gaccgtacac tatatacaga tgactcgcta actaccccac
    57121 ttgcaactat aaacggcgga gagctggcgg agtttctttc tgaaacaaaa aatgcttatc
    57181 agatcaagac aaatgatggc tatactggat atttagccct cgaagatgga accaaagtca
    57241 caaagaatat acaaacaaaa ccggaagagc tatctgatgc agtgattgta ttagatcctg
    57301 ggcatggcgg aaatgatact ggggcattaa gcaatgatga acaaacagaa gaaaaagata
    57361 taacattaag tacagccata aaagtaaaaa aggcgctaga ggatgctgga gcaacggttt
    57421 atttgaccca tactacagat gaattagtac agcttggaga tatatgcgac tacagtgaag
    57481 aaaaaaaggc agatgtcttt atcagtttac acgcagactc tacggaatat gcgaatgaag
    57541 caactggaat cacgacatat tattactatg gacaagagga aacattagca cagacaattg
    57601 ctgactcctt cactgatctg cctctggatt caagaggaat ctccactggg aactatcaag
    57661 ttcttcgaga aaatcttcaa ccttctattt taatcgagat gggttatatg aataatgata
    57721 gtgatttaaa ggaattagta acagacagtt atcagcagca gattgctgca agtttgacac
    57781 aagcattgac cacttatttt cagtaataaa taagcccagc caaacaaaaa tagctcctca
    57841 tatttttcgt ttggccgggc ttattatcgc agatgcaagc ttttgaacac cgtttaagga
    57901 agaggttgtg agcctgccgt ttaactccgc gtcacaagga gcaatttcaa aaaacagctt
    57961 ctcatgtttg atgactattg tgacttgtga ccgaggagtt ggcatgagaa caccgtttat
    58021 tcggaacaag ggactgtgac atgacttttg tcacagttcc attatttttt tagcagcttc
    58081 aaagcttttt ctgtattggc aaaatgaagt ttggcatatt tttcaagcat aggcatgccg
    58141 cctttctcca atagttttgc cgctacttgg tcagatttcg ttccagctcc tgatggaagg
    58201 gtgattccag cttcagctga tgctttgtgt aactcttcca aaaagctggc acgactgatg
    58261 atagaagcag cagcaacagc taaatgatat tgttctcctt tagtaacgaa gtacagtttt
    58321 tcttgtaatt gatttttttc taaacgcgca tattttttaa aatttgcttc cggcgtaaac
    58381 tgatcgatca aaatcgcttc tggtttttct ggctcgattt tttttagcag caaatggatc
    58441 gcttgattgt gcaaagcgac tttcatccga acggcattgt attttggttg gatctcattg
    58501 tatttttctg gagataagac aagcagttta taggggatgg ttttttttaa gatgtcagca
    58561 agctggatga tttggctatc ttttaactct tttgaatctc taacaccaag tttacgtaat
    58621 tctgcaagct gatcacgttt cacataagca gcacatactg ttagcggacc aaaatagctg
    58681 ccatttccga cctcatcact acctaaaacg gataattgac tgatattttt tggcaaagaa
    58741 gaggctgttt ttgctggctt ggcttgttta tcctgtgcct ccccccattt atcagcttct
    58801 attgctgcat tcgtaccttg aaacatcact tttccggatg tataagcggt aatggtcgtc
    58861 ccgtcttttt ttgcagcaaa gctagtataa ggaatctgtt tatttaacaa ataagagcga
    58921 tagtatgctt tcatttgact aagttggtcg tttgttgctt tgataatgac gttgctcata
    58981 aaattctcct cgtttttata cataaaacta taccgcaact cacgactttt cgttttctaa
    59041 gagattgcga gacatagcat ttttttaaag tttatcatat aatagttaag tatgtgaaaa
    59101 atatttgtgg agaacggtga aagaaccaac ttcatggtac actaaataaa actttcatca
    59161 aataggagga aatgattcgt atggcgcacg aaaaaacacg ctacaaagca gtgattgcca
    59221 accaaacata tactattatc ggacgggaaa ccaagcatca tatggatatc gtaacgaagc
    59281 tgatcaatga acagttagca gaattgaaac aattatcacc acaaatggat aatgaacaag
    59341 cagcaatctt gatggctgtc aatgcgcttt cggatcaatt gaagaaacaa gaaagaatct
    59401 tggagctgga agaagaaaca gcagaattaa agaaaaaaat gatcaaattt acagagcttg
    59461 aaaatcgtgt gaaacgcatt gaagcaatcg agaacgaagc tcgggaagta ttgaaagaaa
    59521 atggtcaagc agatcatacc attaaaaatc atgtggaagc acaacagatt ttaaacgaga
    59581 aacgaaaagg acaaattaaa caaaaatctt ctaattaaga tccttttatc aaaataagaa
    59641 aaatttaaaa aagttagaag agaaaggaac gtagcatgct gagtttgctg attttatttt
    59701 tattgctaat tgcgtttttt tctggagcac gtcgcgggtt tgctttgcaa atcatattcg
    59761 cgattggata tgtattgtca tttattgctg cccagcattt ttacaaacca ttagcaagtc
    59821 atttggaatt atacattcca tacccagctg tcaccccaac atcaaagtta gcattttttg
    59881 atcaggtatt tgcttttcac ctagatgaag ctttttatgc aggaactgct tttctgatta
    59941 ttttgctaat tggctggctg cttacccgat ttgtgggggt ttttgtccat ggattgacgt
    60001 atgtgccgat cttgagacag gtagactgga ttgcaggagg catcctgtca ctaatcatgg
    60061 cttacgttac tatatttttg attttgcagt tacttgcctt tgttccatta gtgtggtcca
    60121 aaatcaattc ggcgagcata gtttcgctcg ttttatcgtt gagagaacgc catttcttac
    60181 caacaagatg tatgagcttt gggtaacgaa tattttgaat taaggaaaat agtgctggaa
    60241 aatatttcgt tttttgtaca gagtagttta tttaagaagg ctgcgtagcg tatgtccggc
    60301 acagctcttt tttcatagtg aaagataagg tgataaaatg aataaccgaa tccttgaaac
    60361 attggaattt gaaaaagtca aacagatggt cagacaattt gtagtgactg cacaaggaaa
    60421 agaagaactg gcagaattgg taccagtgag tgagaaacaa acgatcacaa actggcttca
    60481 agaaactgag gatggattga aagtgcaacg tctgcgaggc gggatcccta tacctaaact
    60541 tgaaaatatc cgtccccata tgaaacggat cgagatcgga gcagacttga atggagtgga
    60601 gcttgcacaa gtttccagag tattgtctac gacaagcgaa ttgaatcgtt tcattgatga
    60661 tctttctgac agtgaaatcg aatttgcacg tttgtatatg tgggcagatc agttagtcac
    60721 gatccctgtt ttgagccgcc gtttaaagga agcaatcgat gaagacggac gcgtgactga
    60781 tgacgcttct cctgagttaa agagcataag acagaacatt cggcgaagtg aacaagctgt
    60841 tcgtgaacag ttagatggga tcgtccgtgg gaaaaatgca aagtatttaa gtgatgcgat
    60901 catcaccatg cgaaatgacc gctacgttat cccagtcaaa caagaatacc gcggtgtatt
    60961 tggcggtgta gtccacgatc aaagtgcttc aggacagact ttatttattg aaccaaaaca
    61021 agtggtggat ttgaataatc gtttgcgtca ataccaaatc gctgaacgaa atgagattca
    61081 acgtatttta agtgaacttt ctgctgaact tgttccccat cgtcaagaaa tcatccacaa
    61141 tgcttatgtg attggtaaaa tggatctgat gaatgcaaaa gcacgatttg gtaaagaagt
    61201 aaaagccatc gtgccgggaa tcagtgaaga caatcatgtc gtcttgaaac aagcaagaca
    61261 tccactgatc gatcaggaaa aagtagtttc taatgacatc acaatcggaa aagactatca
    61321 agccatcgtc attacaggac ctaacacagg tgggaaaacg atcacgttga agactttagg
    61381 tctgttacaa ctgatggggc aagcaggatt accgattcct gcaggtgaag aaagtcaaat
    61441 cggtattttt gaggaagtat ttgcggatat aggagacgaa cagtcgattg agcaaagtct
    61501 atcgaccttt tcttctcata tgacgaacac tgtagatatt ttatcaaaag tcaatgaaaa
    61561 aagtctggta ttatttgacg agctcggtgc aggaacagat ccgcaagaag gtgctgctct
    61621 agcaatcgca atcttagatg atcttggtaa aaagtctgct tatgtgatgg caacaacgca
    61681 ttatcctgaa ttgaaagttt atggatacaa tcgggcaaat acgattaatg ccagcatgga
    61741 gttcgatgtg gatacactaa gtccaacgta tcgtttgctg attggcgttc ctggacgaag
    61801 caatgcgttt gaaatctcta gccgattagg tcttgataca gaagttattg atgaagcgaa
    61861 gcagttgatg aatgatgaga gccaagattt aaacgaaatg atcacagatt tagagaatcg
    61921 ccggaaaatg gcagagaccg aatatctgga aatgcgtcat tttgtttctg aggcacaaga
    61981 acttcacgat gatttgaaag aagcttacag ctatttcttt gaagaacgtg aaaaagaaat
    62041 ggaaaaagca aagaaaaaag caaacgaagt cgtgtcagag gcggaagaaa aagcagaaaa
    62101 aatcattgct gatattcgta aaatgcaaca gcagattggg caaggaaatg tgaaagagca
    62161 tcagttgatc gatgcaaaga cccaattagc aaatctacat caagaagaaa ctttgaagaa
    62221 aaataaagta ttgaagaagg cgaaagaaca aaaaacgttg aaaccgggag acgaagtgtt
    62281 ggttacgact tacggccaaa gagggacgct tttacgtaaa aacgggaatc agtggcaagt
    62341 agaaatcggt atattaaaaa tgaatgtgtc cgaagacgag ctgacgcctg ttgctcctca
    62401 aaaagagccg acacaacgag ttatccatgc tgtacgatct gaatctagta gtcacgtgcc
    62461 gaatcaattg gaccttcgag gaaaacgata cgaagaagcg ctaagtgagg tagatcaata
    62521 tttggattct gccattctag ctggttatcc tcaagtgact attgtccacg gaaaaggaac
    62581 aggcgcactt cgcaagggaa ttactgatta tcttaaaaac catcggagcg tcaaaagctt
    62641 tgagtttgca ccggcaaacc aaggcggaaa tggtgcgaca attgttaaat ttaagtaaag
    62701 cttactaaag gtaagcagat ggttcgctat ccaagtatag aaatgctata ctaatcatag
    62761 attttaaaaa tggaggtctt tgatatgtca caagtaatta cagataagac atttaatgaa
    62821 gaaacagata aaggacttgt attgattgat ttttgggcaa cttggtgtgg tccatgtcgt
    62881 atgcaagcac cgattttgga tcagttggaa caagaatatg atgaagaaga attcagaatt
    62941 gctaaaatgg atgtggatga aaatccagaa actcctcaac aattcggtat catgagcatt
    63001 ccaacattaa tgttgaaaaa agatggtcaa gtagtagaaa aagctgttgg tgttcattca
    63061 aaagaacaac tacgtcaaat gatcgatcaa tatttataaa aacttacagc aaaaataagc
    63121 tgagggtggg actgaatcca gttccgtcct cggttttttt gcggttgagt taaggttatt
    63181 caaattaata ttttttgagt ataattacgt tgtcaggctg gattgttttt ttgacagaca
    63241 tgctataata actttaattg aatatgaagg ggagtaccag atgaacgaac gaatcaaaaa
    63301 caaattagct ttacttcctg atcagcccgg ctgttacttg atgaaagata aaaatggaac
    63361 gatcatttat gtcggcaaag ccaaaatatt aaaaaatcgt gtccgctcat attttactgg
    63421 cagccataat acgaaaacgg aacggcttgt tagtgaaatc gaagactttg aatacatcgt
    63481 tacagaatcg aatatcgaag cattgctctt agagatcaat ctgataaaga aaaatgatcc
    63541 taaatacaac attatgttaa aggatgataa aacctatcct ttccttaaga taacgaatga
    63601 aaaatatccc cgtttggtga ttactcgtaa agtactaaaa gacaaagcac tgtattttgg
    63661 accatatccc gatgtcggtg cggcgaatga gacaaagaag atcctcgatc gattatttcc
    63721 tctgagaaaa tgcaaaccta gtcagacgaa ggagccttgt ctttactatc atttgggaca
    63781 atgtctgtgt ccttattatt tcgacgtaga tccgaaagtc tatacgaaaa tcgttgatga
    63841 agtgaaaaag tttttaaacg gaggacacga aaaaatcgag tcagagatcc atcagaaaat
    63901 ggaaaaagct gctgaaaaca tggagtttga aaaagctgca gaataccgag accagatccg
    63961 tgccattgag acaatcatga ctcgccaaaa aatgacaaat actgatttac tggatcgaga
    64021 tgtatttggt tatgcgattg acaaaggatg gatgtgtgtc caagtgtttt tcgtaagaca
    64081 aggaaagcta attgaacgag atgtttcaat gtttcctttc tataacgaag cagaagaaga
    64141 tttcttgact tacctcggtc agttttatca ggaaaatgag catttcattc ctaaagaagt
    64201 cttgattcct gatacgatcg acaagcccag tgtagaagct ttgttagcta cgaaagtgct
    64261 gcaaccaaaa cgaggagaaa agaaaaaatt agttgaattg gcgaataaga atgcctcagt
    64321 tgctttgaat gaacggtttg atctgattgc cagaaaacag gaacggacga tcggagcagt
    64381 tgaacgttta ggaaacgcga tgaatatccc gaccccaata cgtatcgagt cttttgataa
    64441 ctctaatatc atggggacga atcctgtggc tgcgatggtc gtattcattg atggaaaacc
    64501 ggcaagaaaa gaatatcgga aatataaaat aaaaacagtc aacggaccgg atgattatgc
    64561 atcaatgaga gaagtcatct atcgcagata ttcacgagtg attcgcgaag ggttgccttt
    64621 acctgatttg atcttgatcg atggaggaaa aggacaagta gatgtggcaa aagatgtctt
    64681 agaaaatcaa ttaggaatcg atattcctat tgctggcatg gcaaaaaacg ataaacataa
    64741 aacaaatgag ttgctattcg gtatagattt ggctgtagta ccgcttgagc gaaattcacc
    64801 agagtttttc ttactccagc gaatccaaga tgaagtccat cgattcgcaa tcactttcca
    64861 tcggcagcta cgcagtaaaa acagttttgc ttcccgatta gatgaaattg aaaggttagg
    64921 accaaaacga aaaaaaatgc tgcttaaaga gttcaaatcg ttgaaaaata tcacagcagc
    64981 cacgacagaa gaattacaag aaatcggact gccgaaaaat gtcgcacaaa acgtatttga
    65041 taaattacat caggaatgag gaaagggcac tttacaaaga aagtaaagtg tcctatcatt
    65101 ttatgtacaa caaaaagagc gtttacgttt tgttggtaaa taataaatcg cttacgtctg
    65161 gtgtctaagg atatatgtga tttcttgaag aaggagtgaa gaaatgttag aagtcaagga
    65221 tcttgttaaa acttttggga actacacagc tgtcgatcat gtctctttcc aaatccccga
    65281 tggaaaaatc atggggctaa ttggccaaaa cggggctggg aagacaacga cttttcgttt
    65341 gatcttggat tttttgacac aagaccaagg agaagtgtta tggaatggcc atcatcttgg
    65401 ggagaaagat tatgatgtca tcggttatct tccggaggaa cggggacttt atccgaaagt
    65461 aacgattcaa gaccaactgc tttattttgc agaacttaga gggaaatcaa gaaaagagat
    65521 tgaaccaaaa atcgattttt ggatggaaaa atttcaggta aaagggaaaa agacggataa
    65581 agtaaaatct ttgtctaaag gaaatcagca aaaagtccag ctgatcgcca cgttaatcca
    65641 tgaacctaaa ttgatcattt tggatgagcc attcagcggg ttagatccgg taaatgctga
    65701 attgctaaaa gatggaatat tggaattgaa aaaacatggt tcatgtgtca ttttttctag
    65761 ccacaatatg gataatgtag aaaaaatctg cgatcatttg atcatgttgc gaaacggaaa
    65821 aatggttttg aatggaaaag tacatgagat tcgtgaatca tttggccgta cgaagttatt
    65881 cttggaatct ggtttaagcc aacaggaagt agaagaaatc gaaggcgtga aaaaagtgat
    65941 ccttcatgaa gacaatacgt tagagattac cttagaagat cctgaagctg ggaaagaaat
    66001 ctttactcgt gcgactcagt tcggctacat tcctatgttt aaccagcaac cgccaacttt
    66061 ggaagagatt ttcaaattga aagcaggtga aatcgatgcg taagttttgg attattacca
    66121 aagatgtcta tttaaaaaat gttaagtcga tttctttctt gatcatgata ttagtccctt
    66181 ttatcatgct gggtatcatt tatgtagcag gaaactttgc tcaacaaaat agtgatgtca
    66241 ataaaatcgg cgtattggct gataatgagc aaatcagtca gcaaatgagc caagtcaaaa
    66301 cagatgattt tgagttcaaa gcagttgatt ctgaagaaaa agcacaaaaa gagctttcgg
    66361 aagaaaaaat cgatgcgtat ttagtagtct caactgagaa tgataagata aatgggatgc
    66421 tttatagcga aaattcatta ggacaaagta cacaactact gatacaacaa caactgactg
    66481 gcttgcaatc gatgcttcga gcaagtcaat taggcatttc accagaagaa gtagctagct
    66541 tgagccaaca agcaagtttg actcgacaaa aagtcaattt tgattcaaaa ggaaaaatga
    66601 cagttggaga agataactcg acagtccaat atgtcgtaag ttatgtagca acgattgtct
    66661 tgttcattat tattttgacg tatgcgcaga tcattgcaca agagatcgct tctgaaaaag
    66721 gcacgcggat catggaagtg atcctgtcca gtacgactgc acaaaagcat ttttacggaa
    66781 aactaacagg tgttttactt gttgcattaa cgcaaatggc attatacggt attatttttg
    66841 gtataggata cagtcaattc aaagatatgg aaatggtcaa aaatatatta gacggtatct
    66901 cattggataa cttgattgga ccattcttgt ggtattctct tctattcatg atctttggca
    66961 ttttgatttt ttctgtttta gcagctttat gtggctcttt ggtcaacaaa gcagaagata
    67021 cagcaaaagc aattttgcca gtgacttatc tatctctagg cggatatatg cttggattga
    67081 tactaggagc ttcagatcca aacaatatcg ttattcggat cacttcgtat attccattct
    67141 tatcttcata tattatgccg gttcgcttag caaatgagac agtcggtatg ggtggagcat
    67201 tgatttcctt gttggtactt gtagccgtaa cattcttatt gatgatcttg tctgcaaaca
    67261 tgtacaaatc gaatgtactc gtgtatagcg aaggcggatt atggagttca ttgaaacaat
    67321 cgatttcaat tatgagaaat gaacgaaaaa aagcataaaa tatagaaaaa tatagaagga
    67381 agctggacat ttattgttcc cagcttcttt tttgtataga cctatttcta tttttttatt
    67441 atattggata tatatattca ttgaaaatag agcataacaa ctttataatg ttgagtatac
    67501 caatttttgg aggcgtttta atggaccgct atttagattt actttcagag aagttcccta
    67561 caacggaaga tgtcatcacg gaaatcatca atctcgaagc aattttgcag cttccaaaag
    67621 gaacagagtt atttttgagc gatatacatg gcgaatttcc tgcttttgat catattctgc
    67681 gtatcggttc aggaaatctt aaagaaaaga tcaaagacct tttctctgaa caaatgacgg
    67741 aaacggagat cagccaattc actattttta cggcttatcc agaatatgtg ttgacgactg
    67801 attggtacaa aaaacaagat aaatcccagc tgatccatcg tttgatcgat cttttacggt
    67861 ttacgacagt caaatacact cgatcaaaag ttcgaaaagg attgccgaaa gaatatagct
    67921 atatcattga ggaactcttg tatatcgatg atcgtatcaa cgggaaaaaa gactatgtaa
    67981 aaaaaatcat tgaacaactt gtcttgatga aggaagaaga aaaattttta acaaaacttg
    68041 cgcagacgat ccaaaaatct gtcatcgatc atttgcatat cgtgggtgat atttttgatc
    68101 gaggaactca agcagccaaa gtaatggatc gaatcatgga tttttcctct gtagatatcc
    68161 agtgggggaa tcatgatatt ttgtggattg gtgcgtattg cggctctgag gcatgtttat
    68221 tgacgctttt acggattgcc gcacgctaca actatttatt tgaattagag aaagaatatg
    68281 gattaaattt acgtccatta ttttcttttg cccatcaaac ctatcagaag aatccagtat
    68341 ttacccctaa aaacaggaaa gatctatctg aaagagagca agaagaatta gaaaagatcc
    68401 atcaagcact ttctatcctc caattcaaat cagaacatca gttgttgaat cgacgacctg
    68461 aatttaaaat ggacgatcgt aaattgttgg aaaaaatcaa ctatgaggaa tcaacgatcg
    68521 ttttagaagg tcagctctat gagttgaaag ggacttgttt tcaaacgatc cagccagatg
    68581 atcctaaaaa attcttggaa gaggaacaaa aagtgatcga tagtttgatg tattcattcc
    68641 agcactcgtt aagaatgcaa aaacacatga cgtttttgat cgaaaaaggg ggaatgtatc
    68701 tcacgaacaa tcagcatgta ctgttccatg gctgtatacc tgtagatgaa aaggggcagc
    68761 tcttagcatt tgaattagac caatcttatc gcggaaaaga actgctggga ttttttgaaa
    68821 aacagataac tgaaagcgca aaagatttga ctgcaaatga agatttggcg accgatttga
    68881 tttggtatgc gtggagcggg ccattttcgc ctttattcgg caagagtaaa atggcaactt
    68941 ttgagcgcta ttttctcaca gagcctcata cgcacgtcga aaaaagcaat ccctattatc
    69001 acttgcgaga tgaagagtgg ttcagtgaaa aaattttggc agaattcgat gcaaaagaag
    69061 aaggttcagc aatcatcaat ggacacacgc cagtaaaagt aaaaaaagga gagtcaccga
    69121 tcaaagcaaa cggaaaagca tttgttattg acggaggtct atccaaagct tatcaaaaaa
    69181 caaccgggat tgctggctac tcattactat gcaattctta cggtttccaa atcgtgacac
    69241 attatccgtt cttgcctatt gaacaacaat ttgcaaaaaa atctgatcaa acgaatgtaa
    69301 aaaaagtgat cgagcagcag ctcccacgca aacttaatcg gaatacgaca aaaggaatgg
    69361 aattacagca acagattact caactccaaa ggctgttgga ttatcgaaag gacgattgag
    69421 aaaaatgcta gccaacattg ctcaatagac taaaaagcga ctgcaacaaa aggcgtgtcg
    69481 cagtctcctt cggaattagc tcaaaaaacg gtaaaatatt agaagctatt tttctgttta
    69541 tttctctaat actcaggtca gggcgctgat ttcacgacct cttccttaaa cggtgttctc
    69601 ctgccaactc ctcggtcaca agtcacaata tccaacaaac atgagaagct gttttttgaa
    69661 attgctcctt gtgactcaga gttgaacggc aggctcacga cctaatcaga aaaacgatta
    69721 aaattcattc acaaagaaat cgaataaacg ctgttcagat gaatctacac gatgagaaag
    69781 ttccggatgc cattggacac caaggatttt gtgcatatta tctttacttt ggactgcttc
    69841 aatgagtcca tcgttggaaa aggcgatggc ttttaacaaa ggagaaagat ctttgactgc
    69901 ctgatgatga taggaattca caaagtatga atcagggaga aggtcactta ataaactgtc
    69961 cgaaacgatt ttgacttcgt gagttgcaaa ctgcggggca gtgggctgtt gttcgtgttt
    70021 tacggaccat tttggatata atgaaagatc ttgataaagt gttccctcca acacgacatt
    70081 caatagttga agtcctcggc agactgcaaa aatcggtttt ctttgtttca atgcttccaa
    70141 gatcaatgcc tgttcgaatt ggtctcgttg aatatttgtt tccgtcagtt ttggatgagg
    70201 gtcttccatg taaagttgcg gactaacatc ctgaccgcct gcaagaatga gtttgtcgat
    70261 tttctcaatt agttgaggcg ctgaatctgg agacatgact ggcagaataa gaggtaatcc
    70321 ttgagcatct aaaactgcat ccacaaatcc ctgcggtgta tagcttactt gatttccttg
    70381 gaaagtatct gtcgctcgaa tcagttgatt cccggcaatg ccgatgattg gtttcatttt
    70441 tattccccct aaagtagact aattgtaaca aggatttaaa aaaatttaaa ctatgtaaga
    70501 cttgccccta tacttatctg ttcttgctgt gttactatct gtaaagagaa ttttcgtgtg
    70561 aaattcatga cgatagttga aaatattgct taatttgtta taataaaaag taagcagaaa
    70621 taaaaaggtt gggcgtaagc ttcaaggatc tagttcttca aggggtgaga aggacatttt
    70681 attgaagggg cctgcctata ctaagttatc tttagaggta catactttca atgaaaaaag
    70741 aaagcatgta cctctttttt aaatcgaaag gaagtgaagc aagtgttaga agctttggaa
    70801 caaatccgaa aagcagaaga agaaaatgag aagcgtgctc gaaccctacg gcagcagttg
    70861 aagacctatg cagaagaaaa agagcaagaa ttggagataa tccaaacgaa cagtcggcaa
    70921 cagctgaaac agttggtaga agaaaaagaa caattcgaaa agcaagaatt agaaaaagaa
    70981 aaaatgaaat tgaaagcaga agcagacaag gtgcaagcaa ctttgaaaga ccaatatcaa
    71041 aagcatcaga gtcaagcaat cggtgcaatc attgaaaggg tgaaagaaac ttatggccgt
    71101 cactaagatg gaaaaagtga ccctcatttc agataaaaga aatcaggaga ggattttaca
    71161 agcagtacaa ggaatccaaa atgttgaaat ccgggatctg tatcaaaata gaacaaacaa
    71221 ccagtgggta gaaaaatatt ttccagatac cacgattatt gataaagaag caaaattaag
    71281 cgctttaggc aatcggctga aagacatcca agaagctatc caattcatac aacaccacgg
    71341 cgatagccgt caaaagaaac tccatttgaa acgtcaagaa ctttccttac aagacttgga
    71401 agcaaattat tccgaagaaa atttcatgaa taaattagaa gaagtccttg atttaaaaga
    71461 aaagtgggaa cagctaactg aaaaacgtga tcgtttagaa gaagaggaag aatggcttct
    71521 tcattggcaa cagctggata ttgctccaag tatgtatgca agtaagacga ccatttttga
    71581 gatggggact gtgagtgcag caaattttga aagtttcaag gaagaactaa gtaagcttga
    71641 tgacgtttat atggaagaaa tagattatac gccaaaacaa gtttatcttt cgtatgttgc
    71701 tttaaaaaaa tcagttgctg ctatagaaga aattgctggg aaatacgggt tcaataaaga
    71761 aacgtatcct tatgaacaaa atcctaaaga aaaattgcaa gaaaccaaac agcaattatc
    71821 cattgttcac gagcagcaaa aaaagataac gacagcgtta ggtcagtgca gcggctatat
    71881 ccaagaattt gaatgggtag aagaagtcac acttgccgtt gcagaacggg aaaaagtaaa
    71941 agaacggttt atccatgcca cttatctgat cgttttgcaa ggttgggtag atgcagaaga
    72001 aaaacagaat ttgatgcatg tgttaaacga aactgtatca gaagaagata tatacgttag
    72061 tttcgaaaca ccaaactcag aagagattca gcaggaagtc cctactaaat tgaaaaacca
    72121 tccgcttatc gaaccttttg aattgctgac tgaaatgtac agtctgccaa aatatgaaga
    72181 agtagatcca acgccttgga tgacaccatt ttatcttgtc ttcttcggga tgatggtcgc
    72241 agatgttggt tacggattgc tgatgctcat tgggacaata ctagctcaaa aactatttgt
    72301 tttaccccgc gggatgaaac gtttcgtaaa attctttgag attctctcgg taccatcgat
    72361 catctgggga ttgatctaca gttcattttt cggacaggca ttaccaaaag aactatttgg
    72421 tatccgcttg cctttcccaa tcctatctac gacggatgac gtcaatacga ttttgatttt
    72481 atccgtgatt ttcggtctga tacagatctt ggttggatta ttcgtggcag caaaggaaaa
    72541 tatcaggagg aaagattact tgagtgctgt cagtgatggt tttgcctggc aagggatcct
    72601 gattgggatc gttattgcat tgattggtgt catgttgata aaaaatacag catttgttta
    72661 tcttggcgcg gggctatcga tcattgctgc tttatgtatc ttaatcgtgc cgattatcca
    72721 atcgccgtct aaagtcaagg gtgctgccaa aggagcgtat aatctttacg gattgactgg
    72781 ttatatcggt gatttagtca gttatacacg tttgatggca ctaggtattt caggcggaag
    72841 tattggcgcg gcattcaata tgctagttgc tttcatgccg ccagccgctc gattcagtgt
    72901 agggatcctt ttgatcatcg ctttacacgc actgaatatg tttttgacac tgttaagtgc
    72961 ctatgtccat ggtgcacgat tgcagtatgt cgaatttttt ggaaaatttt atacaggagg
    73021 cggacgtgcc tttgacccgc tgaaaacggc ggaaaaatac gtcaacatca atcataagaa
    73081 aaaaaacaat aaataattgg aggattttta tacaatgatg gattacttaa ttagtcaaaa
    73141 tggtggaatg gtatttgcag tattagcgat ggcaacagcg acgatttttt cagggattgg
    73201 ttcagctaaa ggtgttggaa tgacaggtga ggcagcagca gcgttaacaa caagtcaacc
    73261 cgaaaaattc ggacaggctt tgattctgca attgcttcca ggtacgcaag ggttgtatgg
    73321 attcgttatc gcctttttga tttttatcaa cttaggaaat gatatgtcag ttgtacaagg
    73381 attgaacttt ctaggcgctt ctctgccgat tgcttttaca ggtttgtttt caggtatcgc
    73441 ccaagggaaa gttgcagcag caggtatcca aattttagcg aaaaaacctg aacatgctac
    73501 aaaaggaatc atttttgctg cgatggttga aacatatgcg attttaggtt tcgttatttc
    73561 cttcctatta gtattgaatg cctaagcata aaggaggaaa agcagatgaa cgccattgaa
    73621 aaaatcatct ctcagatgaa tgaagcagca gagcaagaaa gagcggcgtt agagcaagaa
    73681 gagcgaatga agatcgatca aaactttgaa caaaaacgta cgcaaatcga aacagaacat
    73741 caaaaacaaa aagaaaaaca aatcgaatta cttgaaaaaa aataccgtca gcttaggaat
    73801 cgccaacaag tagaagtacg acaagaaaat ttgaatgcga agcaggaatt tttacgacga
    73861 ttgtttgcag atgcaatagc tgaaatggag aattgggatg aatctgaaca aatccagttt
    73921 atcaaaaatt cattgtattc attacctttg acaggcaaag taacattcat tgctggagaa
    73981 aaatcagcag cttatctttc tcagacattg ctggatgaat ggaacaatga acttcccttt
    74041 atgatggttt taagtgatga aactgttgct gatcaagcgg gttttctgat aaacgatcaa
    74101 ggagttcagt ataattttct tttcagcagc cttgtacaag atatccaagg aacgatgagt
    74161 tttgagattg ccaatcaatt attcgaataa ggagggatca gtgtatggaa taccacgaac
    74221 taaatccctt gattcgcgga agagagcttg aacttttgtc caatgagaca tttgaacgga
    74281 tgattcaaac aaattctgtc gaagcacttg gtgaaatatt gaaatccacg atttacagtc
    74341 catatattta tgacggatat gaatatgact tcgaacagaa tcttttaaag gagcagagcc
    74401 gtctatttcg ctggttaaag gaatcagcac cggagcctga aatcgtttgg atctatacga
    74461 tgcgctatac tttccataat ctgaaggttt tgacaaaagc cgagatgaca aatcaaaatc
    74521 tggaccattt gtatatcgac gatggatttt attcattaga aaccttgaag gatgccattc
    74581 atacgcaagt ttcaaatgaa ctccctgaaa gcattatgga cagtatacga gaagtacgtg
    74641 attattttga agagtctacc attcttcagg gaattgacgt gatttatgat cgacagtttt
    74701 tgacagagca gcgacggctt gccgataagc taggatatca agaattgctg acagaagtca
    74761 ttgcttttat tgacttgacc aatatcacaa cgatggctcg aggaatattg cagaaaagaa
    74821 catatgggtt catgacgacg gtactttcca gctcgggaag tatcgaaaaa gaagtatttt
    74881 tgaactttgt agataaagat atggaagatt acacgaattt tctattagag acagattatg
    74941 ctgattttct gcgccctgtc atccgtgagg gaaatcttga tctcgtaaag cttgaacagt
    75001 tgaaagatga ttatctaagt tcattgtatc aagaagcaca aacccaggct ttcggaccac
    75061 tgccactact agcctttttg aacgcaaaag aggtagaaag caaaaacctt cgtttactgg
    75121 ttatcggcaa aagaagccat ttcgaaccag aaacaatcag aaagcgggtg agacaagtct
    75181 atgacgcata agatcggtgt agttggcgac aaagattctg ttttgccatt caaattgttt
    75241 ggctttgatg ttcgttatgg tacaaccaag caaaccgttc gtcaggcaat tgaagaaatg
    75301 gcaaagaatg agtatggcgt gatttatgtc acagagcaat gtgcagcatt gagcccagaa
    75361 acgatcgaac gttacaagga acaaatgata cctgctatcg tattgattcc caatcatcaa
    75421 ggatcattag ggatagggct ggaagaaatt caaaaaaacg tagaaaaagc agtaggtcaa
    75481 aatatcttat agtatgaaaa ggagatagaa attttgcaaa ttggaaaaat cataaaagtc
    75541 tccggacctc ttgtaatggc tgagaacatg tctgatgcca gtatccaaga catgtgtcta
    75601 gtgggagatc taggagttat cggagaaatt atcgagatgc gtggcgatgt ggcgtctatt
    75661 caagtatatg aagagacttc tggaattggt ccaggcgaac cggttcgctc tactggtgaa
    75721 gctttatctg ttgaattagg accagggatc atttcacaaa tgtttgacgg aatccaacgg
    75781 ccattagata cctttatgga aatcacacaa agtaatttct tagggcgcgg agttcaattg
    75841 cccgcattgg accatgagaa aaaatggtgg ttcgaaccaa cagtagaagc cggagaaacg
    75901 gtgagtgctg gagatgttat cgggatcgta gaagaaacaa aagttataaa acataaaatc
    75961 atggtgccaa atgggataaa aggaacgatc aaaacaatcg aaaaaggttc attcactatt
    76021 gatgaaacag tatgtgtcat tgaaaccgaa aatggtgaca aaaatttgac gatgcttcaa
    76081 aaatggccag taagacgtag tcggccaatc aaagaaaaat taaacccgga tgcacccatg
    76141 atcactggac agcgagtgat tgatacattc ttcccagtga caaaaggagg agcagcagct
    76201 gtacctggtc cttttggtgc agggaaaaca gtcgttcagc atcaaattgc aaaatgggcc
    76261 gatgtggaca tggttgtcta tgtgggatgt ggcgagcgtg gaaatgagat gacggatgtt
    76321 ttaaatgaat tccctgaatt gattgaccct aatacaggag aatcgttgat ggaacgtacg
    76381 gtattgattg ctaatacatc aaacatgcca gtagcagctc gagaagcttc tatctatacg
    76441 ggaatcacaa ttgcggaata tttccgtgac atgggttatg acgttgctat catggcagac
    76501 tccacttcac gatgggcaga agcacttcgt gagatgagcg gacgtttgga agaaatgcct
    76561 ggtgatgaag gttatccagc ttatcttggt tcacggcttg cggaatacta tgaacgatct
    76621 ggaagagtca ttgcattagg atcagaaggt cgcgaaggaa gtataaccgc aatcagtgct
    76681 gtatccccgt ctggagggga tatttcggaa ccagtcacac aaaatacatt gcgggtcgta
    76741 aaagtattct ggggtcttga ttccagttta gcccaaaaaa gacattttcc ttcgatcgat
    76801 tggatacaaa gctattctct ttattcaaca gaagtaggga agtatatgga tcatatcctt
    76861 caagctgact gggcatccat ggtcaccgag ggcatgcgta ttttgcagga agaacagcag
    76921 ctgaatgaaa tcgtgcgttt ggtagggatt gattcccttt cagataatga ccgtctgaca
    76981 ttagaagtgg caaaatccat tcgtgaagat tatttgcagc aaaatgcttt tgatgatgtc
    77041 gatacattca cttcaagaga gaaacaattc aaaatgttga agatgatctt gacctttggt
    77101 gaagaaggac gccgtgcgtt gtcgttaggt gcttatttaa gtgagatcat ggacggtacc
    77161 gtatcgattc gtgaaaggat cggacgaagc aaatatatcc cggaaacaga attagaaaaa
    77221 ctagatcaaa ttattggtga aatcaagcaa acgatgcaaa cgatcgtttc agaaggaggc
    77281 atgagcaatg attaaagaat atcgtacgat cggtgaagtc gtcggtccat tgatggcggt
    77341 tgaaaaagtt gctggtgtca aatatgaaga attaatcgaa gtccgtatgc aaaatggcga
    77401 gattcgtcgt ggacaagtat tggaagtcca agaagataaa gcaatggtcc aaatattcga
    77461 aggaacaagt ggtatcaatt tacgtgactc ttctgtacga tttttaggcc atcctttaga
    77521 actaggtgta tcggaagaca tgatcggtcg agtattcgat ggtttgggac gaccgaaaga
    77581 taacggacca gagatcctgc ctgaaaagca tttggatatt aacggagaag taatcaatcc
    77641 agtagctcgg gattatccag atgagtttat ccaaacaggt atctctgcaa tcgatcattt
    77701 gaatacatta gttcgtgggc aaaaattacc agtattttct ggctctggtc ttcctcataa
    77761 agaattagct gctcagatcg cgcgacaagc aacggtactc gattcatcag atgatttcgc
    77821 cgtagtcttt gctgctattg gaatcacttt tgaagaagct gaatttttca tggaagattt
    77881 tcgtcaaacg ggcgcaatcg accgttctgt catgtttatg aatttagcaa acgaccctgc
    77941 aatcgaacgt atcgctactc ctcgtatggc tttgactgct gcagaatatc ttgcttatga
    78001 aaagggaatg catgttttgg ttatcatgac agacatgacc aactatgcag aggcattgcg
    78061 agagatttct gcagcacggc gagaagttcc aggaagaaga ggatatcctg ggtatcttta
    78121 cacgaactta gctactttat tcgaacgagc aggaagaatc cgcggactaa aaggatcggt
    78181 tactcaaatt ccgattctaa caatgccaga agacgataaa actcatccaa tccctgactt
    78241 gactggttat attacagaag gacagatcat cttgactaga gagctttaca agagtgggat
    78301 tcagccacca attgatgtac tcccgtctct ttcgcggtta aaagataagg gaactggacc
    78361 cggaaagaca agagaagacc atgcagctac gatgaatcag ctttttgcag cctatgcaca
    78421 aggaaaacag gcaaaagaat tagccgtagt tttaggtgaa tctgctttat ctgatgtaga
    78481 caagatctat gcgaagtttg ctgagcggtt tgaaaaagaa tatgtcaatc aaggattcta
    78541 cacgaaccgt tcgattactg aaacacttga tctagggtgg gaactgctat cgatgttgcc
    78601 aagaaccgaa ttaaaacgaa tcaaagacga catgctggac aaatatctgc cagaagggaa
    78661 gtgagttctg atggcacgat tgaatgtgaa tccgacccgc atggaattga ctcgattgaa
    78721 aaaacagttg acaactgcca caagagggca taaattatta aaagataagc aagatgaact
    78781 gatgcgtcaa ttcatcctgt tgattcgaaa gaacaaccag ctgcgtcaag aaatggaaga
    78841 gtctatgaca gaagcaatgt ctgattttgt attggcaaat gcttcgatca cggaaccttt
    78901 catcgaagaa ttatttgttt tgccggcgga aaacgttgat ttgtctatcg tagacaaaaa
    78961 tatcatgagt gtaaaagtac ctgtgatgaa ttttgaatat gatgaacagc tgacagacac
    79021 acctttggaa tacgggtatt tgaattccag tgccgaactg gatcggtcaa tcgatcggtt
    79081 tactaccctg cttccaaaac ttttggaact gacagaaatc gaaaaaactt gtcaactgat
    79141 ggcgagtgaa atcgaaaaga cacgtcggcg agtcaatgca ttagaatata tgacgatccc
    79201 tcaattagaa gaaacgatct attatattcg tatgaagctg gaagaaaacg agcgcgcaga
    79261 agtaacaagg ttgatcaaag taaaaaatat gggtaccact gactaagtgg tatctttttt
    79321 taaaagagga aaacgatgat taaacaaagc aaacaaataa agaaacgttt gccggctgtt
    79381 cagcttctcg cattaggatt ttttctcctg attttagttg gcggcggcct acttacccta
    79441 ccatttttta gtaatagtgg gcatgggaca aattttatcg atgcgctttt cactgcaact
    79501 tctgctgtct gtgtaacggg attgacaaca ttgaatacag cagagcattg gaacagtgct
    79561 ggtcaatttt tgatcatgct tttgattgag atcggcggtc ttggttttat gatggttccg
    79621 attctatgct ttgcattgat aaagaagaaa gtcagtctca gcacacgtat tatattaaaa
    79681 gaagcgctga atctagaaga agtatcaggt gtgatgaagc tcatgcttta catcctaaaa
    79741 ttggcctttg ttatccaatt gattggtacg gttgctttat cttttgtatt tgtgcctgaa
    79801 tttggctggt tgaaaggtat ctggtacagt ttgttccatg caatctctag tttttgtaat
    79861 gcagggtttg atttgttagg agacagttta gccaatcacc agcaaaatgt ttatttgatc
    79921 atggtggtat ctgctttgat catttctggt gggttaggct tcatcgtatg gcgtgattta
    79981 ctggaatatc atcagaaaaa gaagatgacg attcattcaa aaatcgcatt aaccgttaca
    80041 ggtacccttt tattaggcgg attcgttgtg ttctttttca cagagcgcaa tggggcaacg
    80101 ctagtggaag ggaatatgtt tcaacgattg gcaaacacct tttttatgag tgtgacacct
    80161 agaacggctg gatattattc agttgattac ctccatatga gccatgcagg tctgatttta
    80221 acgatgtttt tgatgtacat cggcggaaca tctgggtcga cggccggcgg attgaaaaca
    80281 actacgttag gtgtgttgct gatccagatg cgttcgatgt tgaaaggacg tacgagagcg
    80341 gaagcatttg gccgtaccat ccgacaaggc gcagtgttgc gggcacttac tttatttttt
    80401 gttacgttga cgttgtgtgt agtcgccatc atgattttat cggtgactga aacgatacct
    80461 aaaacttctg gcatcgaata tattgctttt gaggtcttct ctgcgtttgg tacagtaggt
    80521 cttacgatgg gattgacgcc agacttgact gcaataggga aattgatcat tatagcgctt
    80581 atgtacattg gacgagttgg tgtattgact gtggtcttct ctctgatgac aaaagcacaa
    80641 agaacacaat caaaatacaa atatccagaa gaaagtgtca tgattggata atttcatctt
    80701 ctgacagaaa cctagtgatt tagtaaaata agaaaataaa ataatttttc ttgcttcttg
    80761 gatactttaa gtgcaaaatg ttcgtttatt ttttgaggca ctacccgttg ttgaacctcg
    80821 ggtagtgcct cttttttatt gaaaggaatc aataagattt gattccttta tttatcaata
    80881 ggttataatt catatttttt tattttttct tttacgatac ttagtccaga agctaaaatt
    80941 tcaaggttat tagaggatag ggaaattcga acgaatgcat ctggttgcgc acgtgtagta
    81001 aagcgtctgg aatgataaag tcggataccg ttagcaagaa agtcattctc aattatctta
    81061 gggtcaatct caggatcaac tggaagaatt cgatagtaag ggacacgatt cgttggtttt
    81121 tctactttga aaagagaatc aaaaagctga ttggctcttt ttgtaagact taatttagtt
    81181 tctaagatcc tatttgcttc atttgaatgg aggatttgag tgatgacttc cacatctaat
    81241 ccagaagttt tcacatttat attaaaaatt gcttgttcta tcttgctttg gacatgtact
    81301 ggaactacaa gataagcaac tcgcaatcca gaacaaataa attttgtcat tccagctaag
    81361 tagattgttt ggttaggtaa taaattttgg aaaggaggaa tgatcgtatg ttgatagtaa
    81421 gttgttaaaa atgaatggat atcatcttca ataacccata gaccttcttt ttcaatcact
    81481 tcggctaatt gctctcgtct tttgtgagag atttgaaacc ctataggatt attacaagaa
    81541 ggcataagaa agacaccatg aatcttcttt tttctacatt cctgaatcaa tatatcaggt
    81601 cgcattcctt gctcgtcaaa atcaattgga acgatctcta aatgatataa ctgagccaat
    81661 tcaatgaaat tagcgtatgt atatcggtct gttgctattc tatcacctgg agagaaaagt
    81721 gcagaaagtg tgatagccaa tccattttga acaccggaaa caatagatag agattctaag
    81781 gacggaatga ctccttgagt ttttaaccaa tttgctgcag tagtcaattg aaaatttgtg
    81841 cctttgggat cttggtaatt aagcaaacca acaagatttg ggttattact agttgattga
    81901 ataaacggga caacgaattt attacattct tcaaaagaac taaccagacc aaaatcaatg
    81961 atatgttcgg ttacttcatt ggtagagatg gtgatggact caaagacatt gggagaaata
    82021 aatgtgccac ttccaatatt cgtatacagc aaacctttct caattccata tttataagcc
    82081 ttgccaacgg tagtaaaatt taaatctaaa taatcagcta gctctctttg ggagggtaac
    82141 tgagtgtttt tctgtaattt attctttaaa atatcttgtt ctaattgttg aatcaacgat
    82201 cgataataag gacgcttcaa tttttgtttg tctggtcgcc aagttaacgg gtaattgtca
    82261 taagaattaa taggcatttt ctccactcct taaaattgta taccatacaa ttttaccatt
    82321 gattgtattg attttagaag ttaatctttt gtgaggaggg aattgtctat gaaaattatt
    82381 aattttgagg aagtagtagc ttcttttagt tttgaagaag cgattcaagt aatgaaaaaa
    82441 tgtttttttg attatgaaag tggtaaaatc agccaaaatc ctcgattggt ggaaatttta
    82501 cctgacggaa aaggcaaaaa tatgtttgcg atgatgcctg cttatttggg ggagaacaga
    82561 tattttggca caaaagtcat taccgctttt ccggataatc atcagtatca tttaccttct
    82621 catttaggag agattttctt gtttgatgct caaaacggac tacccaaagc attgataaac
    82681 gcaaatgcag tcacctggat tcgaacagct gctgtatctg cgctggcaac agattactta
    82741 gcaaatccgc atgcagaaac acttgcttta attggagctg gtcaacaagc attgtcgcat
    82801 ttgactgcga tttctaccat tcgtcccatc actacagtgt ttgtttacga tattttagaa
    82861 gaaagacgaa atacttttat cgctaaagct aaaaaacagt atcctaaaat aacgtttata
    82921 aatgcaatat cagtagaaga tgcagtacgt ccagcggaaa tcatttgtac gttgacctct
    82981 agtaaagagg catttcttca aaaagagtgg gtaagggaag acgcacatat caatgctgtg
    83041 ggcacattta ctcctgatac aagagagata agtacatcgt tgatggaaac ttctcaaatc
    83101 tatgtagatg attactcagc tgccttaaca gaaagcgggg acctgcttat agccatggca
    83161 gaaggagatt tagaggaatc ttgtattgtt ggtacattag gcgaattagt ttctgagaaa
    83221 aaaacatttt ataaaggaga atcgaggccg acgattttcg acgcagttgg tctagctatt
    83281 gaagatcttt gttgtgcaga atatatttat aacaaattag agaaaggggc tttaaaatga
    83341 acttagatta ttacgtagtt gatgcatttg cagatgaggt gttcaaagga aatccagcag
    83401 cggtttatgt gctagaagaa tggctgccag aaggtacgat gcaaaaaatt gccattgaaa
    83461 acaatttatc tgaaacagca tttacagtga agaaaaacca agaatttgag cttagatggt
    83521 ttacgccaga tcgagaaatc gatctttgtg gtcacgcgac attagcaaca gcatttgttt
    83581 tatttaacta ttacaaaata cctgatgaaa cgattaaatt ttctactcaa agcggtaatc
    83641 tatttgtgac aagaattaaa gattattatt acatggattt tcctagtatt atgccgaaaa
    83701 aagtgccgat attgtctgaa tatgaagaag cgattggggc taagattaaa gaagcctact
    83761 tagcacgtga tttatttttc gtattagaag atgaaaaaac ggtggctaaa ttgcagccag
    83821 attttacagc tatcaaaaat tttgaattag gaataggagt catcgtgaca gcagaaagtg
    83881 tgcaaaaaga ctttgtttct agaaccttct ttccaaaatt aacaatcaat gaagatccag
    83941 tatgtggatc agcacattct aatcttattc cttattgggc agaaagaatg aataaaacaa
    84001 aattgtcagc ttatcaaatg tcatcaaggg gcggggatct agaatgtgaa ttaaaagaag
    84061 atcgtgtgat aataggagga aaagcagttt tgttttctaa aggtacctcg tttatttgat
    84121 ttctttcaaa tgaattaaaa ataaattggt ggagagtggc aaccttatag ctacaatggt
    84181 tgttaaaaaa agacgcaaac tttttcaatt taatgaaaag tttgcgtctc attttaatta
    84241 ttggtgtttg ttttttattc aatcaagagt aaaatgcccc atactaacta tcctcggatg
    84301 acttcgattt tataaccatc agggtcaatg acaaagtagt aagaaggcgc agtgccaggt
    84361 aatcctttta agtcagtaat ctcaagaccg gcagctttat gttgctcgtg taatttttct
    84421 agatcatcag ctgctatagc gatatgacca tagccgtctc ctagatcata cgcaccatga
    84481 tcatagttgt aagtcaactc taattcatag tcatccccag gaagtgtcaa ataaacaagt
    84541 gtaaatttgt tttctggaaa atcacggcgg cggctttctt caaaaccaaa tgccttttga
    84601 taaaattcga ttgatgcttc aagatctttc actcgaacac atgtgtgagc cattttcata
    84661 gattcatctc tccttttcct tttactcaat cataacaaaa agaagtaagg aaatagttga
    84721 tttgtgcttg ttcatgagaa atttctcatg taaaatggat agaaaataaa actgttaagg
    84781 tggaaactat tcatggataa accaattttt ggtgaaaaaa agcaatttgc tgattatcaa
    84841 acccgttatg cagcatatat tgtcatagaa agagatcagc agattgcagt aatcgaggct
    84901 ccaaatggtg ctttttttct tcctggcggg gaaattgaag gagaagagac gaaagaagaa
    84961 gcgattgtcc gtgagctgat agaagaaatg ggaattgtgg cagaaatttc ttcttatctg
    85021 ggagaagctg atgagtattt ttactcgaat tatcgtgcaa cttattatta taatccagga
    85081 tatttctttg ttgctgattc ttggaagcgt ataggagaac caactgaaaa gaccaataaa
    85141 atttggtggg tgagccctca agaagcgctt gtgaaattga aacgtggtag ccaccgatgg
    85201 gcagtcgaga gatggatcga aaaaaataaa aactgaaaga aagcgagtag atcagctttc
    85261 tctcagtttt tttatttatt acagtacata ttttttgatt gcttcagcta caccgtgctc
    85321 gtcgtttgat gaagtgatga catttgctgc ttctttgaca agagaaacgg catttcccat
    85381 tgctactcct agaccagcat attcaatcat ggataagtcg ttctcgttat ctccgatagc
    85441 catgatttca tcttgatcaa taccaaggtg ctgtgcgaga ttcgcaactg ccgcaccttt
    85501 acttgcttct ttatttagta cttcgaagta atagggcgca cttttgaccg ttgtgtattt
    85561 atcacggaaa gtttgcggta aacgcgcaat tgctgcatct aaaatttctg gttcgtcgat
    85621 catcatcatt ttgacgatgt tcatgtctgg tgtcatttct tcaggtgttc gatattttag
    85681 cggcatcttg actaaacttg cttcgtggat cgtataagcg ctgatatctc gattaggcgt
    85741 gtagatcgct ttgtcatcga tcgaatggag atgactgccg acacggcgtg ccatcacttc
    85801 gatctctagg aaatcttcat gtgtcagtcc gtgacgggcg atgattttac ctgatttcgt
    85861 ttgttgtacg agtgccccgt tataagtaat aacgtaatca ttatcttgaa aaaggttcaa
    85921 ctcgtttaac tgttcctgga cacctggtaa gggacggcca gtgcataaaa cgatcgaaac
    85981 accttttgca acagcagcgg caatcgcttc ttttacttct tttgttactt ctcttttctc
    86041 atttaataaa gtaccatcga tgtcgatagc gactagtttg atagacataa tttcctccaa
    86101 tatattttaa tttatttcaa tcaatgcgcc attacggata tggctggcaa attcttgata
    86161 agtttcgtca aataaatcat aatgatcacg caaggacgga tcaaccattt cttttggaaa
    86221 gaaaaatcgc tcatctcctt gttcttgtcc cgctaatgca gcaacaagct gactaacctg
    86281 tgataattct atcaaagttc cgtcatttcg caaaatctcg atttgtgtgc gatgtctgcc
    86341 ttgttctggg cgataaaaat cataaggtag atcgtagcta gaattcacag cagtgtaata
    86401 aacaggatta tatccgacct tttctactaa caaagtgagt tcttggacta attttgaatc
    86461 ccggttgcca gaaaaagtag cagatttcag tggtttacga ttcaaaaaac gtttagctaa
    86521 atcatttaaa ataggatcag cagattcaga ccattgagta aagtaagtgc ccaagacccc
    86581 atcatcaagt ttgaggtatt cttctaaagt aaagcttccg tcaagaaatg gcagaagtaa
    86641 ttgagaatgg agttcaaaac tgctggcatc ttcttgatac aactcgtgtg cgcgatgaag
    86701 cagatgctcc aaaattactt ccataccacg ggaaacagga tggaagtaca cttgcacata
    86761 catttgatat cggctaacga tataatcttc cactgcatgc atgccactca tggaaaaagc
    86821 gatcccgcct tcataaggac gaatcacacg taatattcgg gtaagatcaa atgttccata
    86881 ttctgttcca gtgaaataag catcccgaag caaatagtcc attcgatctg catcgatctg
    86941 actagaaatc atttggacaa cttgtgggtt ggggtaagtt ttttggatca cactagctac
    87001 cttttcagga aaaccttctt ctactcggtt aagaatctga tacacttctg tttctggaga
    87061 agtgatgatc tctacagtga tggcttcatg attcgtatgg aagatatgtt caaatgtatg
    87121 agagtaagga ccatgaccga catcatgcaa cagtgccgca cataaagtca ccagccgttc
    87181 ctgatcatcc cagcctccgt tgcctatttt ttcttttgaa aaatttcgag aaaagatatc
    87241 gcagattcgt cttgaaatct cataaacgcc taatgaatga gtaaaacggc tatgttctgc
    87301 tccgtgaaaa gtgaaagaag aagtgcctaa ttgtttaatg cgtcgtagac gttggacttc
    87361 tttcgaattg atcaaatcta agattacttg atgctggaca tggatatagt tatggactgg
    87421 atcacgaaaa actttttcca taggcagcat ttgatattta taaggggttg tcataatgta
    87481 gctcctttat gttattacgt tgttccattc tctggatttg gttgttccat tgctccagct
    87541 ggatttcttt ttttagccag tcggtagaat cctgtaaatc acttgcagaa tacagctttt
    87601 caaaagcttt tgcaaatgct tctttgactt cttttacaaa aaggggttgg tcaagcagtt
    87661 cttcaagtgt tgccattgta gcaggatcca ctgcaggata gccatttgta ccaaagtctt
    87721 cttttaaagc agattgataa aatcgcttca tgacttgccc gcgtttttcc tgattaccgt
    87781 tgacacttaa gtacatcata acagaaatcc catttttgat ccgacgctga gcgataccgg
    87841 caaatttttt gccatggatg cttaaatcat aagttccagg acaataagaa tgggtgattt
    87901 cgaatgcttg tatcggtgcc tttggaaaag cgtatttagt caataggagc atttgctcgt
    87961 aagcgtcatc tatggttatt ttttttgtgt cactttgggg gaaaatcaaa gaaatattca
    88021 aaattccttc atctgatacg atccctaatc cacctgaatt tctgatcact ggagcgtaac
    88081 ctgcatcttt cagtgtttgg atcccgtcac ttagaaaagg agttcgaaca tccttcatac
    88141 ctaagatgac tgtgcagtca tattgccaga aatgtaaaaa gatttgctgg tgttctttag
    88201 aatattccgt taatatatct gttaatgcaa aaggggaaaa gtcgtttttt tgataaatgt
    88261 gctgatctag tcgaatcgca gggttcattc aaccggctcc tttgtataaa ttcatatttt
    88321 ctacaatatt atactctaaa atcaaagagt tgttttggaa aaaaggtttt ctgtaaaaat
    88381 ggacaagttg gttgacaaaa caaaagaaac taggaacaat gtagctatga gaagtaaagg
    88441 agaatgaata atggaattat cacaaggaac accgatttca atcaaattgc gtacaaaagt
    88501 aaaacagcaa ggggaagtgc aagattttta ttttgatttg aaagggcaaa tggtgacaat
    88561 tggcgatacg ctgtacatac gttataaaga gattcaggag gagacgggcg atgagattcc
    88621 tgtaacgatc aaattagttc cagatggtca tgtacagctg atccgtgcag gagaaatgcg
    88681 gatgcgtttg aaatttggct ataaagaacg gttagaaaca tcctatcgaa caccatacgg
    88741 tatgatccag attgctactt ttacgaaaga actgcatgtt agtttgaaag accgtcccac
    88801 aggagggaag gttcgaatcg attatgatct gttcatggga ccggaaaaaa tcggcgaata
    88861 ttatttgacc ttggatttta cagcgtaaat ataaaagaaa agtttgattt tttgtgtctg
    88921 attttgtatg ataaggagta gactgtgaaa ggacgtgtcc tgattggaaa ttagtgtatt
    88981 tgaaggtgca aacaaaagcg aattatcaat gatcgaagta gcacacgcga tcttagaaca
    89041 acgtggcgat gtcatggatt tttctgattt agctaaccag atccaaaact atcttgggaa
    89101 atcagacagt gaaattcgtg attcactcgc acaattttat actgacttga atattgacgg
    89161 cagctttatc tcattagggg ataatcgttg gggcttgcgt tcatggtatg ctatcgattc
    89221 gatcgatgaa gaagtaaacc atggaatgga cgaagaggat gaagatactc cacgtcgccg
    89281 taaacgcaag aaagtcaacg cctttatcaa tgatgatgaa gacgcgatcg attacaatga
    89341 cgatgaccca gaagatacag atctgacaga agaagatgac gatgatctgt ttgatgacga
    89401 tgatgatgaa gatgaagaaa tcgctgctta taattctgac ttgcaagaaa tcggagcaga
    89461 tgacccgaca gatgatgaag aagacttgcc aggaaatatc gaagaagatt taagtatcat
    89521 agacgaagac gatgatgatg aagattatga agaggaagaa ttttctgact ctgaaaaaga
    89581 ataaacatta ttttcttacg agaggttgtg acagagacga tcagcctatc agaataagga
    89641 ggtgtgacac gat
//
LOCUS       DARBFO010000004        82862 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000004 DARBFO010000000
VERSION     DARBFO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.004"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            52..570
                     /locus_tag="SCK51_000358"
     CDS             52..570
                     /locus_tag="SCK51_000358"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG1251011.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            698..1381
                     /locus_tag="SCK51_000359"
     CDS             698..1381
                     /locus_tag="SCK51_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEG1251012.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1690..1763
                     /locus_tag="SCK51_000360"
     tRNA            1690..1763
                     /locus_tag="SCK51_000360"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1724..1726,aa:Arg,seq:tct)
     gene            1922..2281
                     /locus_tag="SCK51_000361"
     CDS             1922..2281
                     /locus_tag="SCK51_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEG1251013.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2404..4035
                     /locus_tag="SCK51_000362"
     CDS             2404..4035
                     /locus_tag="SCK51_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEG1251014.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4097..4738)
                     /locus_tag="SCK51_000363"
     CDS             complement(4097..4738)
                     /locus_tag="SCK51_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEG1251015.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4924..5000
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5020..5328
                     /gene="rplU"
                     /locus_tag="SCK51_000364"
     CDS             5020..5328
                     /gene="rplU"
                     /locus_tag="SCK51_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEG1251016.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5355..5699
                     /locus_tag="SCK51_000365"
     CDS             5355..5699
                     /locus_tag="SCK51_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEG1251017.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5712..6005
                     /gene="rpmA"
                     /locus_tag="SCK51_000366"
     CDS             5712..6005
                     /gene="rpmA"
                     /locus_tag="SCK51_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEG1251018.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6439..9246
                     /locus_tag="SCK51_000367"
     CDS             6439..9246
                     /locus_tag="SCK51_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1251019.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9437..9928
                     /locus_tag="SCK51_000368"
     CDS             9437..9928
                     /locus_tag="SCK51_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1251020.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10031..11020
                     /gene="manX"
                     /locus_tag="SCK51_000369"
     CDS             10031..11020
                     /gene="manX"
                     /locus_tag="SCK51_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEG1251021.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11053..11856
                     /locus_tag="SCK51_000370"
     CDS             11053..11856
                     /locus_tag="SCK51_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEG1251022.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11877..12791
                     /locus_tag="SCK51_000371"
     CDS             11877..12791
                     /locus_tag="SCK51_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEG1251023.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12874..13248
                     /locus_tag="SCK51_000372"
     CDS             12874..13248
                     /locus_tag="SCK51_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEG1251024.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13387..13632
                     /locus_tag="SCK51_000373"
     CDS             13387..13632
                     /locus_tag="SCK51_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1251025.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13804..14868
                     /locus_tag="SCK51_000374"
     CDS             13804..14868
                     /locus_tag="SCK51_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEG1251026.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15114..15539
                     /locus_tag="SCK51_000375"
     CDS             15114..15539
                     /locus_tag="SCK51_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1251027.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15532..15981
                     /gene="nusB"
                     /locus_tag="SCK51_000376"
     CDS             15532..15981
                     /gene="nusB"
                     /locus_tag="SCK51_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEG1251028.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16078..16932
                     /locus_tag="SCK51_000377"
     CDS             16078..16932
                     /locus_tag="SCK51_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEG1251029.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16933..18270
                     /locus_tag="SCK51_000378"
     CDS             16933..18270
                     /locus_tag="SCK51_000378"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEG1251030.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18275..18499
                     /locus_tag="SCK51_000379"
     CDS             18275..18499
                     /locus_tag="SCK51_000379"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEG1251031.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18501..19391
                     /locus_tag="SCK51_000380"
     CDS             18501..19391
                     /locus_tag="SCK51_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEG1251032.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19400..20218
                     /locus_tag="SCK51_000381"
     CDS             19400..20218
                     /locus_tag="SCK51_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEG1251033.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20325..20774
                     /locus_tag="SCK51_000382"
     CDS             20325..20774
                     /locus_tag="SCK51_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEG1251034.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20792..22474
                     /gene="recN"
                     /locus_tag="SCK51_000383"
     CDS             20792..22474
                     /gene="recN"
                     /locus_tag="SCK51_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEG1251035.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22529..22645)
                     /locus_tag="SCK51_000384"
     CDS             complement(22529..22645)
                     /locus_tag="SCK51_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEG1251036.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22718..23674)
                     /locus_tag="SCK51_000385"
     CDS             complement(22718..23674)
                     /locus_tag="SCK51_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1251037.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23845..24213
                     /locus_tag="SCK51_000386"
     CDS             23845..24213
                     /locus_tag="SCK51_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEG1251038.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24383..24814
                     /gene="mraZ"
                     /locus_tag="SCK51_000387"
     CDS             24383..24814
                     /gene="mraZ"
                     /locus_tag="SCK51_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEG1251039.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24835..25794
                     /gene="rsmH"
                     /locus_tag="SCK51_000388"
     CDS             24835..25794
                     /gene="rsmH"
                     /locus_tag="SCK51_000388"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEG1251040.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25799..26200
                     /gene="ftsL"
                     /locus_tag="SCK51_000389"
     CDS             25799..26200
                     /gene="ftsL"
                     /locus_tag="SCK51_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEG1251041.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26201..28393
                     /locus_tag="SCK51_000390"
     CDS             26201..28393
                     /locus_tag="SCK51_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEG1251042.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28419..29381
                     /locus_tag="SCK51_000391"
     CDS             28419..29381
                     /locus_tag="SCK51_000391"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEG1251043.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29395..30756
                     /locus_tag="SCK51_000392"
     CDS             29395..30756
                     /locus_tag="SCK51_000392"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEG1251044.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30782..31870
                     /gene="murG"
                     /locus_tag="SCK51_000393"
     CDS             30782..31870
                     /gene="murG"
                     /locus_tag="SCK51_000393"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEG1251045.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31885..33105
                     /locus_tag="SCK51_000394"
     CDS             31885..33105
                     /locus_tag="SCK51_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEG1251046.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33261..34586
                     /gene="ftsA"
                     /locus_tag="SCK51_000395"
     CDS             33261..34586
                     /gene="ftsA"
                     /locus_tag="SCK51_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEG1251047.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34609..35850
                     /gene="ftsZ"
                     /locus_tag="SCK51_000396"
     CDS             34609..35850
                     /gene="ftsZ"
                     /locus_tag="SCK51_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEG1251048.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35867..36544
                     /locus_tag="SCK51_000397"
     CDS             35867..36544
                     /locus_tag="SCK51_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEG1251049.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36558..37157
                     /locus_tag="SCK51_000398"
     CDS             36558..37157
                     /locus_tag="SCK51_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEG1251050.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37172..37459
                     /locus_tag="SCK51_000399"
     CDS             37172..37459
                     /locus_tag="SCK51_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEG1251051.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37508..38290
                     /locus_tag="SCK51_000400"
     CDS             37508..38290
                     /locus_tag="SCK51_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEG1251052.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38311..39018
                     /locus_tag="SCK51_000401"
     CDS             38311..39018
                     /locus_tag="SCK51_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEG1251053.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39280..42078
                     /gene="ileS"
                     /locus_tag="SCK51_000402"
     CDS             39280..42078
                     /gene="ileS"
                     /locus_tag="SCK51_000402"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEG1251054.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42127..43653)
                     /locus_tag="SCK51_000403"
     CDS             complement(42127..43653)
                     /locus_tag="SCK51_000403"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEG1251055.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43668..44315)
                     /locus_tag="SCK51_000404"
     CDS             complement(43668..44315)
                     /locus_tag="SCK51_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEG1251056.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44499..44828)
                     /locus_tag="SCK51_000405"
     CDS             complement(44499..44828)
                     /locus_tag="SCK51_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEG1251057.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45005..45733
                     /gene="sfsA"
                     /locus_tag="SCK51_000406"
     CDS             45005..45733
                     /gene="sfsA"
                     /locus_tag="SCK51_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEG1251058.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45749..46762
                     /locus_tag="SCK51_000407"
     CDS             45749..46762
                     /locus_tag="SCK51_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1251059.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46762..48039
                     /locus_tag="SCK51_000408"
     CDS             46762..48039
                     /locus_tag="SCK51_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG1251060.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48102..50804
                     /locus_tag="SCK51_000409"
     CDS             48102..50804
                     /locus_tag="SCK51_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1251061.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50956..51273
                     /locus_tag="SCK51_000410"
     CDS             50956..51273
                     /locus_tag="SCK51_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1251062.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51303..51623
                     /locus_tag="SCK51_000411"
     CDS             51303..51623
                     /locus_tag="SCK51_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1251063.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51731..53191
                     /locus_tag="SCK51_000412"
     CDS             51731..53191
                     /locus_tag="SCK51_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1251064.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53259..53480
                     /locus_tag="SCK51_000413"
     CDS             53259..53480
                     /locus_tag="SCK51_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251065.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53511..53693
                     /locus_tag="SCK51_000414"
     CDS             53511..53693
                     /locus_tag="SCK51_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEG1251066.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53693..54106
                     /locus_tag="SCK51_000415"
     CDS             53693..54106
                     /locus_tag="SCK51_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEG1251067.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54229..55410
                     /locus_tag="SCK51_000416"
     CDS             54229..55410
                     /locus_tag="SCK51_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1251068.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55481..55645)
                     /locus_tag="SCK51_000417"
     CDS             complement(55481..55645)
                     /locus_tag="SCK51_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251069.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55799..55872
                     /locus_tag="SCK51_000418"
     tRNA            55799..55872
                     /locus_tag="SCK51_000418"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55833..55835,aa:Arg,seq:cct)
     gene            complement(55909..56109)
                     /locus_tag="SCK51_000419"
     CDS             complement(55909..56109)
                     /locus_tag="SCK51_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1251070.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(56414..57646)
                     /locus_tag="SCK51_000420"
     CDS             complement(56414..57646)
                     /locus_tag="SCK51_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEG1251071.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            57900..58469
                     /locus_tag="SCK51_000421"
     CDS             57900..58469
                     /locus_tag="SCK51_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1251072.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(58647..59087)
                     /locus_tag="SCK51_000422"
     CDS             complement(58647..59087)
                     /locus_tag="SCK51_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEG1251073.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            59245..60009
                     /gene="map"
                     /locus_tag="SCK51_000423"
     CDS             59245..60009
                     /gene="map"
                     /locus_tag="SCK51_000423"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEG1251074.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            60041..60964
                     /locus_tag="SCK51_000424"
     CDS             60041..60964
                     /locus_tag="SCK51_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEG1251075.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            61040..62179
                     /locus_tag="SCK51_000425"
     CDS             61040..62179
                     /locus_tag="SCK51_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEG1251076.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            62172..62972
                     /locus_tag="SCK51_000426"
     CDS             62172..62972
                     /locus_tag="SCK51_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG1251077.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            62972..63799
                     /locus_tag="SCK51_000427"
     CDS             62972..63799
                     /locus_tag="SCK51_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG1251078.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            63777..64511
                     /locus_tag="SCK51_000428"
     CDS             63777..64511
                     /locus_tag="SCK51_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1251079.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            64611..65477
                     /gene="rfbA"
                     /locus_tag="SCK51_000429"
     CDS             64611..65477
                     /gene="rfbA"
                     /locus_tag="SCK51_000429"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEG1251080.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            65491..66063
                     /gene="rfbC"
                     /locus_tag="SCK51_000430"
     CDS             65491..66063
                     /gene="rfbC"
                     /locus_tag="SCK51_000430"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEG1251081.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            66085..67113
                     /gene="rfbB"
                     /locus_tag="SCK51_000431"
     CDS             66085..67113
                     /gene="rfbB"
                     /locus_tag="SCK51_000431"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEG1251082.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            67211..68062
                     /gene="rfbD"
                     /locus_tag="SCK51_000432"
     CDS             67211..68062
                     /gene="rfbD"
                     /locus_tag="SCK51_000432"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEG1251083.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            68097..70130
                     /locus_tag="SCK51_000433"
     CDS             68097..70130
                     /locus_tag="SCK51_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251084.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            70174..71454
                     /locus_tag="SCK51_000434"
     CDS             70174..71454
                     /locus_tag="SCK51_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEG1251085.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            71664..72470
                     /locus_tag="SCK51_000435"
     CDS             71664..72470
                     /locus_tag="SCK51_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1251086.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            72482..73702
                     /locus_tag="SCK51_000436"
     CDS             72482..73702
                     /locus_tag="SCK51_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1251087.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            73692..75278
                     /locus_tag="SCK51_000437"
     CDS             73692..75278
                     /locus_tag="SCK51_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1251088.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            75317..77455
                     /locus_tag="SCK51_000438"
     CDS             75317..77455
                     /locus_tag="SCK51_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1251089.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(77823..78806)
                     /locus_tag="SCK51_000439"
     CDS             complement(77823..78806)
                     /locus_tag="SCK51_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEG1251090.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            79184..80575
                     /locus_tag="SCK51_000440"
     CDS             79184..80575
                     /locus_tag="SCK51_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEG1251091.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            80591..81631
                     /locus_tag="SCK51_000441"
     CDS             80591..81631
                     /locus_tag="SCK51_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1251092.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            81650..82735
                     /locus_tag="SCK51_000442"
     CDS             81650..82735
                     /locus_tag="SCK51_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEG1251093.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
BASE COUNT        27099 a        13944 c        17541 g        24278 t
ORIGIN      
        1 atcgttatta tatgattaag aaggattgaa taaagaaaga agttgaaaac gatacctgat
       61 tacaaaaaaa taacttttat aagttctata acgttgatta tatttttaat tcttattctt
      121 ctcgtcgtct tggttccccg aatttttggt ttgactcctt atattattga ggacaaagaa
      181 atgagtccat attatgcaca aggaaatctg atatatgtac gaccgaaaaa tacaaatgag
      241 attttagtcg gagatattat tacatattat gaaaattcag gaaaaaaaat ctcgacgcgt
      301 cgcgtaatag ccgttgaaaa caaccacgaa gatttttata caaaagctga tacaaagact
      361 tatattgagc ctggagtagt aaaaaagaga aatattatcg gtagtcccat ctttcagatt
      421 ccatatattg gactagtcct ttcaaaaacg gcatttgcat ggattcaggg gttattcttt
      481 acgattgctt tttgcctaac aggaatcaca gcatggaata cctttgtaga gttgaaaaag
      541 aagcggagac gaacacagaa atttttgtaa gaatgttaaa aacataaatg tattccagag
      601 atccaacgac tcttctgaac atgaaacacg agaaaacact catgcagctt gtagatttag
      661 tcagttgttc gtataatgtg tgaagaggag gagtagtatg cgcaatgaaa tgatgcatcg
      721 tcctgtagtc aaagaagaac tattggattt tatgcgtacc cagcaaaaaa aattaccggg
      781 tgagctaggc aaactggaag aagaagctca tgtagcagaa gtaccagtta ttccccatga
      841 aacagttgta tttttgaaat ttttacttgg acagcttcag cctgaacgaa tcttggagat
      901 cggagcagct atcggttttt catcaagtgt catggccacg acaatcggag aaaacgctca
      961 tgttacgaca attgatcgtt tcgatgtcat gatcgagaaa gctaaaaaaa actatgaacg
     1021 tctaggactg acagataaag tgactttgct tgaaggtcaa gcagcagata tcttgccgga
     1081 gttgtctggt ccgtatgact ttatttttat ggatagtgca aaatcaaaat atattgaatt
     1141 tttacctgaa tgcttacgtt tgttaagaaa aggtggcgtt ttgatggtag atgacatctt
     1201 ccaaggagga acgatcttgc tcccagatga agagattccc cgtggcaaac gagctatcca
     1261 tcgtaaatta aatgaatttc ttcgtgttgt gatggatcat cccgatttga catcgacaat
     1321 cttgccatta ggtgatggtg tgatcttaat gacaaaggaa tcagaaacaa tcgagttgtg
     1381 atacatgatt atggcagcct gtagataatc gattgaatcg agggctcaat tcgaacttga
     1441 tgtaaaagtt tctatatttg taagtcaatt aaaggataca ggaaatctaa ataaacagga
     1501 gtagcatgaa gtcaatgtag ctcctgtttt caattgataa ccttagaaca gctgaaggag
     1561 aatgtctcgt tttaaaagat tgatttagta gttgggccaa aataattcat tatttgctaa
     1621 ttaaataact atttcgtatt gacagaagca atttaattat ctataattat tgatgttccg
     1681 ctgattcttg tcatagtagc tcagctggat agagcatccg ccttctaagc ggacggtcgg
     1741 gggttcgaat ccctcctgtg acgctaaaaa accgttagaa actgaggttt ctaacggttt
     1801 ttctttgttt tttgtgccaa tattgtttgt tttcaaaggc aaataaaata aaaaaacatc
     1861 cttatcgagt acaattataa ggagcaaaaa aataaaactc atcagaaaag aggaagaaga
     1921 tatgacgata ctatctagaa ttttagtgac tctagttgca attgaatttt tttatatcat
     1981 gtatatcgaa acggtgagaa ctgattctga cacgacaagt agggttttta aaatgagtaa
     2041 agaagagtta agcagaaaat ctgttcaaac attgtttaag aaccaaggag tgtacaatgg
     2101 actattagga gtgggactac tttatggagc ttatctttct tccgcatcta aagaaatcac
     2161 aagtatgcta ttaatatcta ttttttttgt tgcattatat ggcagtttga ctagcgatcg
     2221 ttggatttta ctcaaacaag gtgggctagc tctgttggca ttgctttcta ttttcttata
     2281 aatatagaaa aaataagaat ttatctaaca ggaaactttg tgaaaaatat tactttatct
     2341 catgaaatat gatattctaa tgaatggtat tttagaaaat ttcatgaaaa aggagttaga
     2401 taggtgttca aaaattatgt ttcctcacta aaaaaagaat tttcaggtta tgatctttca
     2461 agatttcaga aagatctttt ggctgggatc acagtagctg ctgtagcatt gcctttagcc
     2521 ttggcttttg gggtatcaag cggagctgat gcggccgcag gtttgattac ggctatcatt
     2581 gccggtatcg ttattggcgg tttatcaggc ggtttttatc aaatatcagg tccgacaggt
     2641 gcgatggcag ctatcttgat gtctattgcg gcaacaaagg gaatgcaagg tgtattgctt
     2701 gctacttttt tagcgggtct atttttactt gcagcaggac ttttccgatt aggaacactg
     2761 acttctttta tcccagcacc cgtaattacg gggtttactt caggtattgc tatcattatt
     2821 gcattaggtc agatcgataa tttatttggt gttcactcgg aaggtacaaa cgttattgaa
     2881 aaactaagta gttatcaaac gataggattt ccgataaaca cagcctcttt gatcatggga
     2941 gggctagtca ttttagggat gtttatctat ccaaaaaaat gggcgaaacg aatgcctagt
     3001 tcgctgctgg ctatcatttt ggcaacagca gttatgctat tgttccattt accagtagca
     3061 actgtgggta agattccaca aacattgatc agtagtaatc gtttgaatat gggagatttt
     3121 agcttttctg ccttgcaaca agtggcagtt ccagctatta gcattgcttt attaggaatg
     3181 attgaaagct tgctatgtgg tgcttcggct ggccgaatgg caaataagcc cttagatagc
     3241 aatcaagaat taatggcgca aggaattgga aatctgttgc ttcctttctt tggcgggatt
     3301 cctgctactg cggccatcgc ccgtacaagc gtggccatca aatctggtgc acaaactaga
     3361 ttagctggta tgatacatgc agttgtctta ctgctctcca tgcttgtttt tgcgccagtg
     3421 atgtcacaga ttccaatgcc agcacttgca ggtgttttga ttgtgacggc ttggcgtatg
     3481 aatgaatggg aaacgatcaa ggagctattt actaataaat attggtcagc agttctattg
     3541 tttatcctta cgatgatttg tacagttatc tttgatttaa gtattgctat cgtgatcggg
     3601 gtgatcggcg gctgtgtatt ttttgttgtc aaaagcgctg ctatcacgat ttcattagaa
     3661 acgatcgatt ggcaaagaat ggggctgcca gaaacggaaa agctgaataa ctggacagta
     3721 gtgtatatta gtggtccact gttttttatg tcttcagaaa aactcaaaaa taaattggaa
     3781 gaactaaatc atcacgatgg tgtcattttc tccatgcgag gcgtaccaag tattgatttg
     3841 acggcacgtg cagtttttga tgaatttcaa gaaaaagcag agcaaagagg acaaacgatt
     3901 atctatactt ccttgcagcc agaagtagaa aaacaacttt ctcacttgtg ggaacataca
     3961 ttcacgcatc aacatcaaac agttgctcat gcgttgacag ctttacatgc gaaatatact
     4021 gcaattagcc attaaaacaa tgaaaaagaa cagacaagac tgtaaactga agattttatg
     4081 gtcttgtctg ttctttttaa tattgctgta aaagtgaagc tagtacttgc cagtcttgat
     4141 ggataacagg tacaagggag cgattgtaga aaatagctgc tggatgaaaa agtggaagaa
     4201 ttcgatagtt tttttctgac cattcgtagg catccttttg ttctgttaat tgtaagacag
     4261 gccctgtata gagttgacca tgatttttgg taaccgtata ttctttgcca agtaaacgtt
     4321 gaagaccaat gtttccaagt gtcgcaatta attttggctg gaccttcatc agttcgtaat
     4381 ccaatattgg tgcatgcgca aatacttcgg aacgagtagg tgtccgattt ggataaacag
     4441 tttctgactg ttgtgtccgt ttattgatcc gatgagtcac gcgatacggg cggctcctta
     4501 cagcactggt gatatacacc tcttctcgtg ttaagccagt tgaagaaagt gcctgcatca
     4561 attcttttcc agcttgaccg ctaaaaggaa catgcgtatc aatctctgtt ttccctgggg
     4621 cttctcccac gagcatcagt ttaggatgaa gaggtccttg cccagcgata aatccttcta
     4681 caggacggcc gttcattctt aaagaagctt cttgtatcag ttcttttggg tattccattt
     4741 atgctcctcc tttttctttt attataagaa taaaagaaac acaatgcgac tattttattt
     4801 ggtagaactg tgaggataga aaagcagaag tattgcttga atttttgttt tactttttga
     4861 ttttaaattt agttgactaa cggttgctgt tctggtaagc tagtactgtt gtaattgtgg
     4921 actccacagc tacaaccgct cagaacaagt aataagcaca gcatatgctg tcacttcgga
     4981 tggcgagtct aagtatatca gaaggaggtg ctttatagca tgtacgcaat tattaaaact
     5041 ggtggtaaac aagtaaaagt tgaagtggga caagcaatct atgttgaaaa acttgacgtt
     5101 gaagcaggag aaaaagttgt ttttgacgaa gttatcttag taggcggaga atctacaaaa
     5161 gtaggcgctc caactgttgc aggtgcaact gttgaaggta ctgttgaaaa acacggcaaa
     5221 caaaagaaag tcgttacttt caaatacaaa cctaaaaaac attcacaccg taaacaaggt
     5281 catcgtcaac catatacaaa agtggttatc gacgcgatca acgcttaata gcgtttgaga
     5341 aagaaggcct atatatgatc aaaggaacat ttaagcgaaa tgacgcaggt cagatcgttt
     5401 cattcacttt gaccgggcat gctgatgctg gtccttatgg aagtgatatc gtttgtgcgg
     5461 gtgtttctgc gttagcgatc agcactgtca acgggattgc ctcattagcc ggttttgagc
     5521 cgattgttga aatgaatgaa gaagaaggcg gttatcttta taccgaagtc acttcaggaa
     5581 tgacacaaga acaaaacaat attgctgaga ttctattaga aaatctttta ttaggattac
     5641 aatcgataga agctgaaaac tcagaatata tccaaattaa aacaattaat gataaatagg
     5701 aggtgcagac catgttgtta tctatgaatc tacaattatt tgcccacaaa aaaggtggcg
     5761 gttctacatc taacggccgt gactcagaat caaaacgtct aggtgctaaa cgtgctgatg
     5821 gacaaacagt tacaggtgga tcaattcttt accgtcaacg cggtacaaaa atctatccag
     5881 gtgtaaacgt gggtatcggt ggagacgaca ctttatttgc aaaagttgat ggcgtagtac
     5941 gtttcgaacg taaaggccgt aacaaaaaac aagtgtctgt ttatccagta gctcaagaag
     6001 cttaattgaa tcttatgatt aaagaggttg tgacaagact tttgtcacag ccttttttgt
     6061 ttataaaaat aaaagttgta gtggaggcta cgaagtttac tttgtctttt tacacttaat
     6121 atttttttgc caatttggaa tcaggatgtt ttgataacta tcgttttcaa ttgatggaag
     6181 ctatattgtt tgctatcagt gaaataatta tatggtaaac agtgttcttt ttcttgtttc
     6241 cattataaaa atcgtatcgg gatttttcgt tcttttaaat tgaacatttc tatctatccg
     6301 cgtgttatca acaaaacaaa atcaggtaaa tagttgacga aacagaaata aatacatagt
     6361 attatgtttt gaagcctaag tacaatagtc tatagaaaag gagcgatacc attggcaaaa
     6421 cgcattgacc gaatttatat gtatgtcaaa gaaaaaaccg ctcatttaac aaaggcagaa
     6481 tatgatcaag gagtaacaac acaggaaatt gctgaagtgt tagggattca gcgaacaaat
     6541 agcagtaaag atctgaatca gctagtgcgc gaaggaaaac tgataaagac agacggacga
     6601 ccagtacgtt acatatatca aacactcgtt acgcacgtaa agccagttac aaaacatgta
     6661 gtcagttaca aagaaacgcc ggtctctgaa gaaaaaaata cactccaaat agatactaag
     6721 gatatctttg caaaaatcat tggagctaac ggcagcatga aaaattcagt ggaacaggca
     6781 aaagctgcga ttttgtatcc gccaagagga ttaaattgtt tgattacagg acctaccgga
     6841 tcaggaaaga cttattttgc acacgcgatg tatcattttg caaaagcgca tcatgtaata
     6901 gatgaggaaa cggaactgat tgttttcaat tgtgctgatt atgccaataa ccctgagctt
     6961 ttgatgagtc atttatttgg gtacgcaaaa ggtgctttta caggcgcaga tgaagaaaaa
     7021 atggggatta tcgatcaagc ggatggcggt atgctgtttt tggatgagat tcaccggttg
     7081 ccgcctgaag gtcaagaaat gatcttttac tttatggatc atggaactta cagtcgtctt
     7141 ggggaaacaa cgaaatccca tgaagcaaat gtacggatcg taggtgcgac cacagaagac
     7201 ccaggttctt ctctattaga aacttttgtt cggcgtatcc caatcaatat caaacttccg
     7261 gcttttgaaa aacgtcctgc aaatgagaaa gtcgatttag taaaaatcat gattgcccac
     7321 gaagcaaacc gcacgcagcg aaagatttct ttgacagaag atgtagcaaa agcattgatc
     7381 ggcagtgtga cctatggaaa tataggacaa ttgaagtcaa atatccagtt agtatgtgca
     7441 cgaggatttc taaatcatat gcaatcaccc gaaatctcga tcacaatcga tgatttgaca
     7501 gaagggattc gtagcggatt gattcaatta gcaagcaacc gcagtgcaat ggcagagctt
     7561 tctaaaatat tggagccaaa gataactgta gcaccaaatg atacgatcat gaaaatccaa
     7621 tcggactcct atgagttacc ttataatcta tatgatatca tcggagataa agcagcttta
     7681 ctgaagtcgg atggtttgga tcaagaagca atcaatcatt tcatttctac agatatcaac
     7741 atccatctga agtctttcta taaagaccat ggtttttctt ttaatgcaga cagcaaatta
     7801 gcagaatttg tagatccgaa aatcatagaa gtgaccaatc agatttatgt aatggtcaaa
     7861 agatctttac catatgagtt tcagcaaaac tttatttatg ctatgagctt gcatatcagt
     7921 tcttttttga aaaaaatcaa cttaggagaa gaacggcata cgaatgataa catccgtgag
     7981 atggcttttg attttccaga tgaatatgct gttgcgaaag aagtgcgtag ttatatcgaa
     8041 acgtattttc aagtggagat tccagaaagt gaagactatt acttgaccgt tctactcgtc
     8101 tcgttacggg caaatcaagc aagcggcaga atcggtgtag tcgttgcagc tcacggaaac
     8161 agcacagcaa gcagtatggt ccaagttgtc caacagttgt tagatgcaga gaacgtacga
     8221 gcagtagaca tgcctttgga tatggatccg aaaacagcat tggaccgaat tgaacgcaat
     8281 gtacaagaag tagatgaagg aagcggggtc atactcttag tagatatggg gtcccttgcg
     8341 tcgtttaatt cgcaaattca aagagacact ggaattccag tacgtactgt tgatatggta
     8401 acaacttctc tagttttgga aactgtacga aaggtctcag tattagggac agatctagat
     8461 atgttgcatg aatcattgaa aaattttcga ggttacgcag aaatatcttc tgtagaatct
     8521 gttcaggaag caaaacctat tcataagaaa aaagctgttt tggctatctg cgcatctggc
     8581 gaaggtacag cacaaagaat caaagaattg atcgaacgag cggtaaacaa gcgtcaagaa
     8641 acagaattag aagttctagc tttgtcgatt gtggaagtga aagaagaact gccagttatc
     8701 caaaaagaat atcagatcat agctactact gggattactg atttgaatat ccaagcccca
     8761 tttattcctt tagaacgttt tattgatcaa aatatcgaag tcattttgga tcagttgctg
     8821 atggaatcag aattggagga aactgaattc atctccctag atgaagagtc agctaaaaat
     8881 acgtgtgttg agtttatctc tgataacttc atctttatta atggtagtaa gttaatcgat
     8941 ccaatgtggc aattttctac acaaatctct cagacgacgg gaatcggcga tgaagaatat
     9001 ggattcaaaa tcaacctagt gatgcacaca gcaggaatga tcgaacgtat tattcggaat
     9061 gaaccattga ctgtggaaga aaacgaattg acaaatacaa cgaatgatcc tctttatagc
     9121 caattggcag cctcagttgt tctgttggaa gatcagataa aagtaaaagt gccgatagaa
     9181 gaaatgtatt atctgctacg tctagtccat aatcagttag ataaaaaaga atacactgtg
     9241 ccatgaatac acaaaaacga tacactttta tagtgtatcg tttttgtgta ttggttaaga
     9301 atcctttaaa ttcaattagt gtatcgtaaa aaagttggca cgctttttgc ttatagtagt
     9361 gtgtgctagg aggtggttgt tcacacagaa atgatagggg tttctgtgaa gtagtgagga
     9421 agaaggagtt ttgcagatgg atattcgatt ggcacgtata gatgatcgtt taatccacgg
     9481 gcaagtcgca acagtttggt cgaagcaaac aggtgttgaa cgtatcattg tgatcagcga
     9541 tgtagtcgct caagataaat tgagaaagtt tctactaaaa gaagctgcac cgccggggat
     9601 cacagcaaat gtaataaccg tgcagaaaat gattcaaatc tatcatagtg gattattatc
     9661 acgagcaaaa gtgatgcttt tattcaccaa tccccaagat gttgaagcgg ttgtccgctg
     9721 cggtgtacag ttgaagtcgt tgaatatcgg cggtatgagt tttactgaag gcaaatcaat
     9781 gattaccaat tttgtttcag taaatcaaca agatattgat tcctttcgtt acttgaacag
     9841 tcaagaagtg gagttagaga ttcgaaaagt acctgctgac aggaaaatcc ttttgatgga
     9901 actactagcg aaaatcaaaa aggtgtagca agaatggttg ttggaaaagg tcaaataatt
     9961 ctgttgtaat ggttagcaat tattaatatt tttgaatttg ttacaacaaa agtaaatagg
    10021 aggtataaat atggtaggga ttatcctagc aagtcatgga caatttgctg aaggaatctt
    10081 acaatccggt tcaatgatct ttggcgaaca agaaaacgtc aaagccgtga ttttgaaacc
    10141 aagtgaaggt ccagatgact tgagagccaa attggaagaa gcggttgctt cttttgataa
    10201 tcaagacgaa gtgttattct tagttgattt atggggcgga acaccgttca atcaatcaaa
    10261 cacattattc gaagagcaca aagataaatg ggcaatcgtc agcggtttga atttaccaat
    10321 gttgattgaa gcctacgcat cacgcttttc aatggaatct gctcatgaaa ttgcagcaca
    10381 catcattgaa acagcaaaag acggtgtaaa agtaaaaccg gaagaattag aaccggcaga
    10441 agcaccgaaa gcagcagttg aagacgcaca acctaaagga gcacttccag aaggaactgt
    10501 ggtcggagac ggaaaaatca aatatgtatt agcacgtgtt gactcacgtc tgttacatgg
    10561 acaagttgcg acagcttgga caaaagcagt gcaaccaaac cgtattatcg ttgtttcaga
    10621 tgcagtatca aaggatgatc tgcgtaagag actaatcgaa caagctgctc ctccaggtgt
    10681 aaaagcaaat gttattccta ttagcaaaat gatcgaagta gcaaaagacc ctcgttttgg
    10741 aaataccaaa gcattgcttt tatttgaaaa tcctgaagac gtattgacag ctgttgaagg
    10801 tggcgtagac atcaaagaat taaatgttgg gtcaatggcg cattcggtcg gtaaagtcgt
    10861 tgtaagtaaa gtattgtcta tgggacaaga agacgttgaa gcgtttgaga agcttgaaca
    10921 aaaaggtgtg aaattcgatg tacgtaaagt acctaatgat tcacgcgata acatggatga
    10981 tattttgaaa aaagcaaaag ctgaattagc aaaagcgtaa taaataatat taaaatttat
    11041 aggaggctta tcatgtctat tatttcaata attttagtcg tattagttgc ctttctagct
    11101 ggtatggaag gtattcttga tgaatttcaa tttcatcaac cactagtggc atgtacattg
    11161 atcggtttag ttaccggtaa cctcgaagct ggtatcgtac ttggcggtac gcttcagatg
    11221 atcgctttag gttgggcaaa tatcggtgcc gctgtagcac cagatgctgc attagcatca
    11281 gtagcatcag caattatttt agttttaggt ggacaaggtg ttaaaggtgt tccttctgcg
    11341 atcgcgattg ccgttccttt ggctgttgca gggttgttct taacaatgat cgtacgtaca
    11401 atcgctgttc caatcgttca tttgatggat gcggctgctg aagaaggaaa catccgtaag
    11461 gttgaaatgt ggcacatcat tgctgtatgt ttacaagggg ttcgtatcgc ggtcccagca
    11521 gcagcgttgc tatttatccc agcacaaact gtgcaatcat tccttgaatc aatgccagca
    11581 tggttaacag atggtatggc tatcggtggg ggaatggtcg tagctgtagg ttacgctttg
    11641 gtaatcaaca tgatggctac aaaagaagta tggccattct tcgttattgg tttcgttgta
    11701 gctgcaattt ctcaattaac attgattgct ctaggtgctt taggtgttgc attagctctt
    11761 atttacttga acctttctaa aatgggtggt tcatcaaata atggaggcgg aagcaactct
    11821 ggtgaccctt taggcgacat tttgaatgac tattaatctt gaaggaggaa aataaaatgg
    11881 cagaagaaaa aatcaaatta tcaaaaaaag atcgtttagc tgttgcgtgg cgctctacct
    11941 tcattcaagg ttcatggaac tacgaacgta tgcaaaacgg tggctgggca ttttctatga
    12001 ttccagcaat caaaaaatta tacaaaacaa aagaagaacg ttctgcagca ttaaaacgtc
    12061 acttggaatt ctttaataca cacccataca ttgcttctcc aattttaggg gttacattgg
    12121 ctctggaaga agaacgtgcg aatggcgcgc ctgttgatga tgtagcgatt caaggggtta
    12181 aagtaggtat gatgggtcct ttagctggtg ttggtgaccc agtgttctgg tttacagtac
    12241 gtccaatgct aggtgcacta ggtgcttcat tagctatggg aggaaatatc cttggaccaa
    12301 tcatcttctt cttaggttgg aacattattc gttgggcatt catgtggtat acacaagaat
    12361 ttggctacaa agctggttct aaaattactg atgacctttc tggcggattg ttacaagacg
    12421 taacaaaagg tgcttcaatt ttagggatgt tcgtattagc tgcccttgta caaagatggg
    12481 tatcaatcaa attcttacca gttgtatcaa ctgtaaaatt agataaaggt gcttacatcg
    12541 aatgggataa attgccttca ggtggtgaag gaatcaagag tgcatttgaa caagttaata
    12601 atggcttagc cctttctcct gaaaaggtaa caacattaca agacaactta gaccaattga
    12661 ttcctggttt agctgcatta ggattaacat tgttatgtat gtggttgctt aagaaaaagg
    12721 tttctccaat cgttatcatc cttggtttat tcgtagtcgg tgttgttgga cacgtcatcg
    12781 gacttttata atccataaaa atatagctcg ggcgttgtcc cgagcttttt ttatatagta
    12841 tactgttcat aaacaaatat ctggagggag aaaatggttc aatcaatcaa tacaaaagta
    12901 gacttggtaa ttgatgctac ttcgtttacg ggtctgacag attacgggca aatcatgata
    12961 ggtgataatg gttttgagtt ttaccatgcg cgagatagac gcaaatatat ccaaattcca
    13021 tgggaagaag tggattatgt actagcatca gtcatgctaa agggaaaatg gattcctcga
    13081 tatgccattc aaacgaaaaa aaatggtaca tacacatttt cttccaaaga acctaaaaaa
    13141 gtgcttcgtg cgatcagaga atatgtagat gaagatagaa tggtacaatc attgagtttc
    13201 tttgatgtag tgaaacgtgg tattcgtagt atgtttaaac gcaagtaaaa aataaagaaa
    13261 acttctaaga ggaggttttc tttatttttt tactttgcag aagaaaatat gaaatagcaa
    13321 aaccaattat ttgtcccctt taaatcccat atgctaaaat tgattagcaa agaaggaggt
    13381 tatattatga gttggttatg gacattgatt gttggagccg tgatcggagc aattgcaggt
    13441 gcgatcacca gtaaaggaaa ttcgatgggc tgtgtaacga atatcattgc tggactagtc
    13501 ggttcaagta ttggacaagc gattttcggc gaatggggac caaaagctgc aggtatggca
    13561 cttattccat ctattttagg cgcagtgatt ttggtagcag ttgtctcatt tttctttgga
    13621 aaacgatcat aagtttttac aaaatagtaa actaaaagga gttgcagcga gactgtgact
    13681 cctttttggc tttatttatt taaaataaaa agaagttttc cggaaatttt tatttaactg
    13741 tcgtgtacga agaaaagttt gctataatgg aaaaaagcgt gcaaagaaag agggagtttc
    13801 attatgatgc tacgtgtaga aaaattgcgc aaaaaaatgc aagaagaaaa tttggattca
    13861 tttttggtta ccagtccgta caatcttcgt tacttaacga actttacagg tacgacagga
    13921 ttagctgtta ttactttgga aaaagcattt tttattactg attttcgtta taccgagcag
    13981 gcggcagcgc aggctcaagg gtttgaaatt attaaaaatg tcggacctat ttttgatgag
    14041 gtagcaaact tagtacgcaa agaagaactg cactcattag cttttgagga aacaacagtc
    14101 tcatttttag aatattcggt tttagaagaa atcatcgatg cggaattagt acctgtttca
    14161 cagatgatcg aagaactcag agaagtgaaa gatgaagaag aaatcgctat tatcgaaaaa
    14221 gcatgtcata ttgcggatat ggcatatgat cacatcctga aaatgatcca accgggcatg
    14281 actgagattg aagtcgcaaa tcaactagac ttttatatgc gttctcttgg cgcttcaggt
    14341 gtttcttttg atacgatcgt tgctagcgga ttgcgttcgg ctatgccgca tggtgtagca
    14401 agcaaaaaaa tcatcgaaca aggtgacttg atcacattgg actttggttg ctattacgaa
    14461 gggtacgttt ccgatatgac acgtacattc gctattggtg atcctgggga aaaattaaaa
    14521 gaaatttatc aaatagtctt agatgctcaa ttagcagtta ttgatgctgc aaaaccagga
    14581 atgactggtg tccaattaga tgcagttgct agagattata tttcaaaaca tggttacggg
    14641 gaagcatttg gacattctac aggacatggg atcggtttag agatccacga aggaccaaac
    14701 gtttcttctc gtgcagaaaa gcaatttgta ccaggaaatg tgatcactga tgaaccaggg
    14761 atctacttac caggaatcgg tggagtacgt attgaggatg acttgttgat cacagaagat
    14821 ggaaatcgtg tgttgactcg ttcaccaaaa gaattgatca tcttatagtt tcatcaatag
    14881 aagaagtaag cgtaaaaatc agtgttcaca gactttgtta tagaaaatca ctcgatttaa
    14941 tgatacacta tagaagaact tttaagctgt aagagataat aaagttgggt gttttctcca
    15001 attcatagca gtttgcagct gatcggcggc agttttctta ccacgtgatc actatgccgc
    15061 atcatagaga attcggatgt atctcgtgct taggcaaagg gaggaataaa taaatggcag
    15121 atgaaaaaaa tttgatttta aacgcaaacc aagatttagg agagatcgtg atcgctcctg
    15181 aagtcatcga agtaattatc gggattgcag cttctaaagt agaaggcgta tacggaatgc
    15241 gcggaacttt tgccagcaat gtcactgaat tattaggacg tgcagctcac ggaaaaggtg
    15301 tttacttagc aaacgaggaa gatggcttga aagtagatct ttactgttat ctagaatatg
    15361 gtgtttcagt tcctaaagta gcaatggaaa tgcaggaacg ggtcagccag caagtccttt
    15421 tcatgacgga tatcgattta tctgaagtca atatccatgt agtagctgtt gttcctgaaa
    15481 aattagaaca accaacaatc gaagaacttt ttgaagacga agaggaagag aatgaataaa
    15541 gaattatcaa gacacgagat tcgggaaatg gcattgcagg ctttatttcc attagacttc
    15601 aatgcagacc taacaaaaga agatgcgatt ttcaacgcaa tcgaattaga ccaccgcgac
    15661 atgatcaacg aagatgaatc agagtttgtt cctgtttatc ttgatacgct agttggcggt
    15721 gtctgtgcaa aaaaagatga attggataaa gtgattgaaa aacatttaaa aaacaattgg
    15781 cacatcaatc ggatctcaaa aatggatttg gtgatcttgc gtatcgcgat ttttgaaatg
    15841 acttatgtga cagatgtacc agcggctgtt gctttaaatg aagcaatcga gttggcaaaa
    15901 acatttagtg atgaccgttc acgtaaattc gtcaacggtg ttttatccaa tgtattgaaa
    15961 gaattagaag ctggggcgta agtcctagct tttttctatg aatcatcata aacctgttat
    16021 aaatcgggaa aattctatgg tagaatgaaa acacaaaaaa tatctgggag tggttagatg
    16081 gcagaattga ttaacggaaa agaattagct gaaaaaatgc aggcagagat tgctgaaaaa
    16141 atccaaaaat tgaaagataa tggtatccat cccggattag tcgttttatt ggtaggggaa
    16201 aatccagcca gccaaatcta tgtaagaaat aaagaacgtg tagcaaaaga aatcggaatc
    16261 cattcattgg tagagcgtta tcctgagacg atttctgaag acgaactact tgcagaaatt
    16321 gaaaaataca atcaagatcc tagttttcat ggtatattgg tccaattacc attgccaaag
    16381 catatagatg aagaaaagat cctgttagct atccgccctg aaaaagacgt agacggtttt
    16441 catccgatga atttaggcag actccttgcg ggtaaaccgg acaagattcc ttgtactcca
    16501 tacggcatca tgaaaatgtt tgaagcttat tctatcgatc cggcgggtaa acgagcagta
    16561 gttatcggac gaagcaatat cgtcggtaaa ccgatggctc agctactctt gatggcagat
    16621 gcaactgtta ctgttgctca ttctaaaacc gaaaatctgg ctgaactggc acaagaagca
    16681 gatattttag tcgtagcaat aggacgtgga cattttgtca caaaagaatt cgtaaaacca
    16741 ggtgctgtag tgatagatgt agggatgaat cgtgatgcta acggtaagct gatcggtgat
    16801 gtgaaatttg atgaagtgga gcctattgca ggctggatca ctcctgtgcc aaagggtgta
    16861 gggccaatga ctatcacgat gctgatgtat caaaccgtaa aatctgccga agcttttgga
    16921 gcaggtgaat agatggcaca agaatattta actgtcacta cattaacgaa gtatttgaaa
    16981 agaaaatttg atgccgatcc ttatttagaa cgcgtatatt taacaggcga aatatcgaat
    17041 ttccgtttac gtgccaatgc ccatcaatat ttcagcttaa aagatgacca tgcaaaaata
    17101 tctgctatta tgttcaaagg agcattccaa aaattacgat tccagccaaa agaaggaatg
    17161 aaagtccttg tcgttgggcg tatttcatta tatgaagcgg gaggttctta tcaaatctat
    17221 gtagagcaca tggagcctga tggaataggt gctttatatc aagaattgga agaacgaaaa
    17281 gaaaagctga tgaaggaagg cctttttcaa gggcctaaaa aaatgctgcc acgtttccca
    17341 aagcgaatcg cggtccttac cagtccaagc ggagcggtta tccgcgatat catcacgacg
    17401 gtgaaaagaa gatatccaat tgtccaattg gttttgttcc caacagttgt acaaggaaac
    17461 caagcagcgg acaatgttgt caggaatatc cagagagtag aagagatggg caatttcgat
    17521 acaatgatca tcggtcgtgg gggtggttcg attgaggatt tatggccgtt taatgaagag
    17581 cgggttgctc gggcgattta tcaagctaat acgccggtga tttcatctgt aggacatgaa
    17641 acagatacca cgatcgctga tttagtagca gatgttcggg ctgccactcc aacagcagct
    17701 gcagaattag ctgttcctgt tttgtcagaa gagttgatgc gcattcaaga acggcaagct
    17761 agattgcagc aggcgtttgt acgccagatc cagcgcaaac aagaacggtt cgagcgtgct
    17821 cgacgttcct atgtatttcg gcagcctgaa agattgtatg aaggtcagac catcaaatta
    17881 gaccagttga aacagcgatt atatcaagct acacagcaaa tttatcatgc gaaagaaaaa
    17941 caaacgatcg cattaagcca ccaattggaa caagtggctc cgctttatcg agttaaagca
    18001 gctcgccaag aaactgctta tctagaaaaa cgcttagaag agaaaatcca acaatatatg
    18061 aaacaacagc agcagcgttt tgctcaagct gttcaatctc ttgatttgtt gagtccattg
    18121 aaaatcatgg gaagaggcta cagctatacg acgttaaatg atcaagtagt aaaaagtgtc
    18181 accgagattc atcaagggga tttgctgacg atccattatc aagatggaat cgttaaagga
    18241 caagtacaag agatagacaa aggagaataa aaatatggaa aatccaactt ttgaagagtc
    18301 actgcaagaa ttagaaaaaa ttgtcatgca gttagaacaa ggagacgttc cattagaatc
    18361 agctctagat tcgtttaaac gaggaatgga attaagcaaa cattgccaag atacattagc
    18421 taaagcagaa gagaccttga cgaaaatgat gacagaatca aatgaagaag ttgattttga
    18481 cggaaacgag gaaagctgag atgagattaa ctgacttttc agctattcat ctacctgctg
    18541 tagaaaaaga aatcttgtct tttttagatg agcatacaac ggatagagga ctctatgatg
    18601 cgatgagtta ttcagtgaaa gcaggcggga aaagaatacg tcccttatta cttttaacgg
    18661 ccgttgcttc ctttgatgag cctattgatg ttcccgtcta tcaagtagca gctgctttag
    18721 aaatggtgca tacgtattcc ttgatccacg atgatttgcc tgcgatggac aatgatgatc
    18781 ttcgtagagg aaaacctacc aatcataaag tattcggcga agcattagct atattggccg
    18841 gagatggttt gcttacagga gcattccagc taatcagtat ggcacactta ggtaattctc
    18901 ctaagcttct gctgcttcag caattagctg tttgtgcggg atcacaaggt atggttgctg
    18961 ggcaagctgc agatatcgag ggcgaatcga aaaagctttc tttagaagaa ttagcattta
    19021 ttcatgaaag aaagacaggg catttgattc gttatgcctt gctggctggt ggcatattag
    19081 cgaagcaacc tgaagaaatc ttattgctac ttcaacgatt agccgaacat ttaggtttag
    19141 cttttcagat aagagatgat ctactcgatg tgattggtac aacgaaaaca ttagggaaaa
    19201 cagctggtaa agatgaacgt atggaaaaaa atacttaccc gcgtttatta ggcctagaaa
    19261 agacacgtga ggcgttggag attgagctgt tatccgccaa caagatcata gacaaattag
    19321 aagaggacgt tttttctttt gatggcgggc tgatgcgcca gatgatcaag caatttgatg
    19381 tggagaaatg agggaaagaa tggaaaaaga acgcgtagat gtattagcgg tcaaacaagg
    19441 attatttgaa acaagagaac aagccaaacg ttcagtgatg gctggtttga tttacaacga
    19501 aaaaaacgag cggtttgata aaccgggaga aaaaattcca gtgagtagtg aattaagagt
    19561 caaaggtaaa aaacttcctt atgtttctcg agggggatta aaattagaaa aagcactcaa
    19621 agcatttgat ctatctgttg aaggaaaaac attgttagat attggtgcat ccactggcgg
    19681 atttactgat gcagctttgc aaaatggcgc aaaaatgagt tatgctttag acgttggcta
    19741 caatcagcta gcttggaaaa taagacaaga tcctagagtc gtcgtaatgg aaagagtgaa
    19801 ttttcgttat gcaaaacccg aagactttac cttaggaatt ccagaagttg ctgttatcga
    19861 tgtttctttt atttcactaa aactgatgct gcctccatta catgctattt taaaagaaga
    19921 gggagaagtg attgctttaa tcaaacccca attcgaagca ggcagagaag ctgtagggaa
    19981 aaacggaatt gttcgtgacc ctcagaccca tcaaaaagtt ttagaagaca ttttgtcatt
    20041 tgcggcacaa ggtggatatg atgtgctgga attaagttat tctccgatca cggggggaga
    20101 aggaaatatc gagttcttgg ctcacttgag gaaagtaccc gaaagcggaa caatcaattc
    20161 agctattaat atggcagaag tggtttcaaa tgcacacgaa cagtttgacc ataaataaat
    20221 tgtaactgtg aaaatcaagt gagaagtatg cggattcttc tttattatac caaggaaata
    20281 agctatcatt gattaagaag aatatgctaa ggagcgaaga cgtaatgaga aaaaaagatc
    20341 ggcatcgttt gattactcga ttgttgagtg agcaagatat ccgaaagcag gaagaatttg
    20401 ttgaaatcct gaaaagcaaa ggaatatctg ttactcaagc tacgatttcc agagatataa
    20461 aggaactgaa actgatcaag gtaccggctt cagatggcgg ctaccgttat agcataccag
    20521 ctgaaacaaa cgaagatgta aatactaaat tggaaaaact attaaaagac gctttcgtgg
    20581 cagttgacca aatggaaaaa tttgttattt tgaaaacatt gccgggaaat gcttcggcag
    20641 cagcaaattt gattgataaa cgttacaaga aagaattatt ttcgattatc aatgatgacg
    20701 ataatgtttt gatgattaca cgaactgaag accaggcagt tttattgaag aaagacttcc
    20761 tgcattacct ttaagttgag gagcgaatgt gatgctacaa gaaatcagta ttactaactt
    20821 tgcgattatt ccggaattgc ggctttcttt tcacgaagga atgaccgcgc tgaccggaga
    20881 gactggtgcg ggtaaatcca ttattatcga tgctttaggt ctgttagcag gaggaagagg
    20941 ttcaagcgat tatatacgtc aaggagcaga aaaatgtgtg cttgaagggt tgtttgaact
    21001 accaaaacaa gaaggattct ctgaactgat ggttgaatta gggatcgaaa cagatgagga
    21061 caatctgatt gttcgacggg atatgtcctt aactggaaaa aatgtttgtc gagtgaatgg
    21121 tcatattatc acattagcta atctgagaag aatcggcagt tatttagtag atatccaagg
    21181 acaaaacgaa catcaggaac tgctccagcc tgaatcacat cttgctttat tggatcgttt
    21241 tggagatgta gctttccaac agaagaaaaa gagttatcag caagaatata tctcttatcg
    21301 tgagctggaa aaaagggtac gaaaagtgca gcaaaatgaa aaatcctatg ttcagagaat
    21361 agatatgctc catttccagc aagaagaaat agctgctgct gatttgcaag tcggagaaga
    21421 agagaaatta aaagaagaaa gagaaaaact tagcaattat caaaaaattg ctgatggatt
    21481 agctgcaggt tacggagcac tgacgaataa tgaacaaaac agtgtagatg gtgttggctt
    21541 ggccgtttca gaactgcaag ggatcgccca tctagatgtg gagtatgaag caatttatga
    21601 aaatatccaa agtgcttact atttgctgca agatgcaatc ggcgatatga gtcgtcagat
    21661 tgatttattg gagttagatg agagtcgttt agaagaagtt acgcaacgtt tagaattaat
    21721 ccgacagttg aaacgtaaat acggcgaatc cattgagtca atattggctt actatgacga
    21781 aatcaccgaa gagctagctt cttctgattt ttcagaaggt caattggaca aaatgaaaga
    21841 ggaactagag caaaaagagc ttctacttca gcaacaagcc gcagatcttc atgaagcgcg
    21901 taaaaaaatc gcaaaagaat tggaaaaatc cattctgcat gaattgaaaa gtctgtatat
    21961 ggaaaacact gagtttgagg tacgtttctt aaaagaagaa aatagacaat tgaatcatga
    22021 tggatttgac caaatcgaat tttacattac gaccaatcca ggagagccac tgaagccttt
    22081 ggtgaaagtc gcttctggtg gcgaactgtc tcggatgttg cttgctttga aaactatttt
    22141 ttcttctgag caaggtgtga caagtattat ttttgatgaa gtggatacag gtgtgagcgg
    22201 aagagttgca caggccatcg cagataaaat ttctcaaata tcgaaatact ctcaagtttt
    22261 gtgcatcact catctgcctc aagttgctgc agtcgccgac tatcaatatt atattgtgaa
    22321 agcagtgatt gatggacgta cgcaaacttc tgtttcagag ttgaaaacaa aagaaagaga
    22381 agaagaaatt gcacgtatgc tggctggaag tgagattaca aaactcacac tcgaacatgc
    22441 aaaagaacta ttgaaactcg caaaaaggac ctgagccacc tcgcagtggt tcaggtcctt
    22501 ttcagcagct gatagctata ttttattttt acatgacact cattacagca gtcaatacaa
    22561 cagatccaat cgttaaaatc agcattgtcc aaatgacaaa ttttgttact ttagcaaaag
    22621 tacttgtttg ttttttttca cgcattgttt tcaacctcgt cattaattag ttgattcaag
    22681 tgtacaatct tttttccatt tccacaagag ggctttttta taaaaacttc ccttttttta
    22741 aataataggt agttacgata caaataaata atgttaatag aactagccat ataaaggcat
    22801 catccctttc agcaataggt aacttaatgt tcattccata gatactgccg acgattgtcg
    22861 gtatagttaa aataatcgtt aaagaagtta aaattttcat aatattattc agattattcg
    22921 atacgattgc tgaaaaagtc tgattcattt gttctactag ttttaattga atgcgagaag
    22981 tagtcattgc ctgctctgtt tccaccaata catcacgtaa atgatttttc aaagcatgat
    23041 tttcttggaa aagtttattt tgcgctagtt ctttcaatgc atctaaatta gcagaagtcg
    23101 cttctctgaa ataaacgagg cttttttgga tatccatgat ttgataaagt tgtttgtttt
    23161 cagtggatac ttgaagtttg gcttccaaag tatcgcattg agattgaagc gtagctaaat
    23221 aactgttgta agagagtgtg acctgccaaa tgaattgaaa gaagatattg agattaggcg
    23281 aaatatcact ttttggaaag gtctggctaa agactttctt taaaaacagt ggttcatgat
    23341 tcgtaattgt gataagcttc tttttgggcg tcaaaataat agagagcggg tatgtatcca
    23401 cttgaaaata accgctagga cttgttttag caaaaggata ttgtaacaat aataacgctg
    23461 gctttgtcaa ctttgattga ttgaattctt ctgtacggga attttctgca tcgtccaata
    23521 tggaagtgat gtaatctgac ggaatttcaa attgatgtgt cagctgatga atctcttctt
    23581 gattaggacg ttctacacaa taccagatcg tttggtcagt ttcctttccg gtattacgca
    23641 gtttctcatt atctaatgta aaatatttga tcatgatttt cctcctgttg cctgtttctt
    23701 ttattataac ggatagggaa ttggaataca tggaatgtgt ttgtaagata tagaatataa
    23761 ctgcataaat ttaaataaaa tgaaacagtt gtgtaaagta tatacagtat acttgaagca
    23821 acgataaaaa aggagtttac gttcatgggc acattcacac cacttatctt gttttggtat
    23881 atttacccgg taatcgtttt gtttggttgc caatttcttg taaaattatt ttcgttaaat
    23941 cgtcgtttta aaataaaagc ccctgatttg gcgattcctt ttttgtgggg cggtatgcat
    24001 gcattgtcta gaaatacagg gacaatttct atcttaccgt ttttctttat ttctgttttg
    24061 ctaatgggaa tttgtattgc tgttttccaa gcatattatt atgaagaaat tatttatccg
    24121 cgttatttca agatgacttg gcgtttggtt tttctgttga caatcgtctt gtacgctgtc
    24181 ttgatcattg tgaacatact ttcttaccta tgaaaaaaat agaaaagctg taaaaagtct
    24241 tttaaaaggc tttttgcagc ttgattttta aaaaaatgtt tatgaataaa aaaagttgtt
    24301 cccggatttc ccagaaaagg tggtagaaag tgggggattg tggtagacta attttagtta
    24361 gtggaggaat ggggggcttt caatgttgat gggtgaattc caacataata ttgatgcaaa
    24421 aggacgttta atcgtgccct caaaacttcg tgaagaactt ggcgaaaagt ttgttctgac
    24481 acgcggattg gatggctgtc ttttcggcta tccgatgagc gaatgggaaa atctggaagc
    24541 taaactgaac gaaatgccgt tggctaaaaa agacgcacgt acttttgtgc gattcttcta
    24601 ttcggctgcc actgaatgcg aacttgataa acaaggacga atcaatatac ccagcacgtt
    24661 aagaaattat gcagctttga caaaaggttg cgtagtcatc ggtgtatcta accgaattga
    24721 aatttgggat gagacacgat ggcaagattt ttctgcagca gcagaagaga actttgacga
    24781 aattgctgaa agtatgattg atttcggact ataagaatag gaagaggctg actaatgact
    24841 gaaacatttc aacactatac agtcatgttg aaagaaacgg tagatggatt gaacatcaaa
    24901 ccggatggcg tttatgtaga ctgtacactc ggcggtgccg gacatagtga atatttatta
    24961 agccaactaa gttctaaagg gcatctgtat gcgtttgacc aagatcaaaa agcaatcgat
    25021 cacgccaagc tgcgtttagc ttcttatgtc gaacaaggta tggtcacatt tatcaaagcg
    25081 aacttcagag aattagaaga acgcttgagt gagcatgtag ataaggtaga cggcattttg
    25141 tatgatctag gggtttcctc tcctcagcta gatgaagcag aacgtggatt tagttatcat
    25201 caagatgcac cgttggatat gcgcatggat cagtcagctc ctttatccgc ttatcacgta
    25261 attaatgaat attcctacca tgaattagta aagattttct tccgctatgg cgaggagaaa
    25321 ttttccaaac aaattgctcg tgaaatcgaa cgagtacgaa aaacaaaacc aatccaaaca
    25381 actggtgagc ttgtcgaaat cattaaacaa gtcatcccag ctcccgcaag aagaaaaggc
    25441 gggcatcctg caaaaagaat ttttcaagct gtgcggattg cagtaaatga cgaactcggt
    25501 gccgaagagg cctctttaga acaggcaatc cggttattga aagtcaatgg aagaatcagc
    25561 gtaatcacat tccattcatt ggaagacaga attgtcaaaa gtatgtttaa agaatatagt
    25621 acggtacaag atctaccacc gggacttcct gtcatacctg acgaattcca accagaactg
    25681 aaagtcatta ctcgtaaacc aatccttcct tcagaggaag agctaacaga aaacaatcga
    25741 tcacgcagtg caaagttacg tattgcagaa aaaataagaa tgaaagagga gtagaatgat
    25801 ggcagaatta aagaaaatgc aagattacga atatgattta cctgaattgg tggatcagcc
    25861 ggaacaacaa atgcctgatt cttcttcgaa tcaagaaatc attcttccac aatcaccggc
    25921 aaaacgatta aagcagattt ctgctttgga aaaaattgct gtggcaagcg tggcgattgc
    25981 tattgttgct ttatgtgttt taacagttat gctgcgcaca aatatcagcg gagctgaaaa
    26041 agaaatcacg tcaatacaaa cagaaatcaa tgataaaaag caagaaaaaa cgagtttaga
    26101 acaagaaaaa aacgaacttt ctcgtactga acggattaaa caaatcgcag aagaaaaagg
    26161 attgtctatt aatgacgaca atttaaggaa agtgaagtaa atgagtctta aaaataaaat
    26221 tcggcggttt atggaaaaga aaaatctaaa tccgatgaac aatcgcaaaa aagtaggtat
    26281 cattttattt gctacgagta ttggattgtt ctttttattt gccgtccgtt tctcgtatat
    26341 cgttattgga ggtcatgttg ctggtacctc attagctgaa aaaacaaaac agttgtacca
    26401 aggaagtgaa gtagtaaaag caaaaagagg aacgatttat gatcgtaacg gtgtggcgct
    26461 cgcagaagat gcctcctctt attcaattaa agcgatactc tcaaaaacat atacctctgg
    26521 ggataaaaaa ctgtatgttg aagaaaagaa ttttgataaa atagcagaaa tcctgcataa
    26581 gaatctttct atagacaaaa aagatgcact gaatatttta gaggacggcg caaaaaaaga
    26641 gctgtatcaa gttgaatttg gtagctatgg gaaaaatatc tcccaagaaa ccaagcaaaa
    26701 catcgaagcg gacatgaaaa aagaaggtgt cgctggtctt tattttgtcg accatcaggc
    26761 tcggatgtat ccaaatggcg tcttttcttc tcattttatt ggatacgcag ttccagacaa
    26821 agatgagaat ggcttagttg gtaaactagg tttggagtct gcttacaatg atatcttgag
    26881 cggtaaagat ggtaagatta tttatcaaaa agataatttt caaaatcctc tgccaggtac
    26941 agtggcagaa gaagaaaaag ctgtcgacgg acaagatatc tacacgactt tagacagcag
    27001 actacaaagt tatttagaaa cactgatgga tcaagtaaat gaagaatatc agccagaaga
    27061 attgacagca gtcctgatga aagcaaaaac cggagaaatc ttggcaatgg gacaacgacc
    27121 tactttcaat cctgaaacaa tggaaggatt gactggaaaa gatgctatct ggcgtaattt
    27181 cttggtacaa gatagttatg aaccaggatc aaccatgaaa gttttcacta ctgcagctgc
    27241 tatcgaagaa ggagaattca acgagaatga aaccttccaa tctggaaaaa tccaagtaga
    27301 agatgctacg atcaatgacc atgactttgg tgaaaaaggt gtgctgacca tgcgtcaagc
    27361 tctttcatgg tcaagtaacg tcgggatggt catactggaa cagcgattag gcggacgatg
    27421 gtataactat ttacaaaaac tgggatttgg acaaagtact cattctggat tagatgatga
    27481 agtaaacggt gctttaccta cattaaatat tgtcgaccga gcaatgagtg cttatggaca
    27541 agcggtaggt gtgacgaatt tccaaatgat gaaagctttc acttctattg cgaataacgg
    27601 tacaatgatt caacctcgat atatcagcaa agtggtggat ccccaaactg gagaagagcg
    27661 aacaactcaa acagaagttc tagggcaacc tttttcaaaa gaaacaacag aaaaagtccg
    27721 tgaatatatg agagatgttg tcgaaagcga aaactacggt agtgcttatg gtgtatatag
    27781 tgtacctgga tacaatgtat cggcaaaaaa cggaacagcc caaatcgctt ctgatacagg
    27841 gggatatcaa acaggagata cagcctacct gtattcgatt gtggagatgg ttccttctga
    27901 agatccagac tatgtcttgt atctaacaat gaaacaccct aagacatatg atcgaatggc
    27961 attggctaaa atcgcaaatc cattgatgaa acgggcaatg gattttaaag aaactgaaga
    28021 agattcagat acagaaacaa aaacagaaaa agtatccgta gccgattatc gtaatcttga
    28081 agcggacgtt gcagcagctg atgctcaaaa aagtggactg caacctgtag tgatcggtaa
    28141 tggtaaaaaa gtacaaaaac aatctactgc caacggtgat cagttgatct ctggagaaaa
    28201 actgatccta tatactggtg gtgataagct gatgcctgac gtaacaggat ggtcaaaagc
    28261 agatatcatg aagctgggga aaatcctcgg tattgaagtt agttttgatg gagatggtta
    28321 ttgtgtcaaa caagaacttg ctccatatga aaaaatcact gaagacaaac ttagttttac
    28381 tttagaagaa taagaagaga aataggagag aaaagagcat ggaatggaca caagcattga
    28441 ttcccattgt cagcagttgt gcaatgacaa ttgccgcaat gccgctattt attggttatt
    28501 ttcaaatgaa aaagcaaggt caggcgattc gtgaggaggg accaaagtgg cacaatagca
    28561 aagccggaac tcctacaatg ggcggcttag tgtttctgat cggctctatt ttgacaggaa
    28621 tttgggttgg agcttggcaa aaacagctaa ctcctactct tttcatcttg ctttttgtat
    28681 tagcgttata cggagtaatc ggctttcttg atgattttat aaaaatattc aagaaaagaa
    28741 acatgggact gaactcgaag cagaaattga ttggacagat tctaggggga atcatctttt
    28801 atcttgttta ccgttctgaa ggttatccag gtgtattgaa tttcttcgga ctagatctac
    28861 ctttaggaat catttatggt attttcgctc tcttctggct tgttggattt tcaaatgcag
    28921 taaatctgac agatggcatc gatggattag ttgctggctt aggaagtatc tcttttgcag
    28981 cttacggtct aattgcttgg catcagcatc agtatgatgt attaatcatt tgcttaagtg
    29041 ttttaggagg tttactgggt ttcttcgtct acaacagaaa acctgccaag atttttatgg
    29101 gagatgtcgg ttcattagcg ttgggcggac ttttagcggc aatttctata atgctaaacc
    29161 aagaatggac attgcttttg attggtttga tctatgttat ggaaacagca agtgtgatgc
    29221 ttcaagttac ttcctttaag cttacaggaa aacgaatttt taaaatgtct ccaattcatc
    29281 atcactttga aatgtgcgga tggtcggagt ggaagattga tacagttttt tggttgatca
    29341 gcattgtgac ttcgttgatc acattatgga tcgtttggta ggagatgacg ggaaatgaaa
    29401 aaaatatcaa catatgaaaa taagaaagtg cttgtcttag gactcgcaaa aagcggagta
    29461 agtgcggcca aattattaca tgaattgggc gcacttgtca ccgttaatga tggaaaacct
    29521 tttgacgaaa atccggaagc acaagagctg ctttctttag ggataaaagt aataacaggt
    29581 agtcatccaa tcgaattgtt ggatgaagaa ttttcattga tggtcaaaaa tccagggatt
    29641 ccctacagtc atcctttagt agcaaaagcc caagaaatgg gtattccggt aattacggaa
    29701 gttgagctag cttacgaagt agcggaatgc ccaattattg ggattacagg aacgaacgga
    29761 aaaacaacga caacaacgat gacaggacta ctactgaatg caggagcaga tcaaggaatt
    29821 gcacgtttag cgggtaatat tgggtatcca gcaagtggtg tagcgcaaga agcaaaatca
    29881 gaagataaaa ttgtgatgga attgtctagc ttccaactca tgggaatcac agatttccgc
    29941 cctcatatag cggtgattac caatatctat gaagcacata ttgattatca tggtacaaga
    30001 aaagaatatg tcaaagctaa atggaacctt caaaaaaaca tgacagaaaa agattatctg
    30061 atccttaact ggaatcagtc agaactacaa gaattagcgc aaagaacaaa agcacgcgta
    30121 ctgcctttct cgacaaaaga agtgttggaa gacggtgtgt atgctgacga ttactcaata
    30181 tactataaaa aagaaaaaat aatggaaata tctgaacttg gcgtaccagg taagcataat
    30241 gtagaaaatg cactagctgc tatttctgta gcaaaattgt atggtatatc aaatgaagct
    30301 atacgagaaa cgttgcattt tttccatggc gtaccacatc gtacgcaata tgtaggagaa
    30361 atccaaggca gaaaatttta taatgattca aaagcaacca atattttagc gactaaaatg
    30421 gctctaagtg gatttgaaac gtctaaggtc gtgttattgg ctggcggatt agacagagga
    30481 aatacatttg atgaattaat tccttcatta aaaggaatca aagcaatggt cgtatttggc
    30541 caaacaaaag aaaagttgat ggatgctgga aaaaaagcag gcatcgagac aatagtaaca
    30601 gccgattctg tagaacaagc agttccttta gcattagaaa acagtacaga tggtgatgta
    30661 gttttattat caccagcgaa tgccagctgg gatcaatacc caaattttga aactagagga
    30721 aacagattca tggaagctgt gaaccgatta aaatagaaaa agtagaagcg agcgataaaa
    30781 aatgaaaatt ttggttacag gcggaggaac agggggacat atttatccag ctcttgcctt
    30841 tgtcaattat gtaaagactc aagaaccgaa tgctgaattc atgtatgtgg gagcaaaacg
    30901 aggtctagaa aacaaaatcg tccctgatac aggaatgcct ttccatacct tggagattca
    30961 aggatttaaa cgaaaaattt ccatgcataa catcaagacg gtccgattgt ttcttaagag
    31021 tatccgtcaa gcaaaaaaaa tcctcagtga ttttcaacca gatatcgtta tcggtactgg
    31081 aggatatgtt tccggagcgg tagtgtacgc tgcagccaaa atgggtattc caacagttat
    31141 ccatgaacaa aacagtgtac caggtgttac caataaattt ttaagccgat atgtggatcg
    31201 gatcgctctt tcatttaaag atgcggcttc cttttttcct gaaaacaaag cagttcttgt
    31261 aggtaatcct cgagcacaag aagtagcaga tactaaaaaa tcagatattt tgaaaacatt
    31321 tgatttggat ccagaaaaga aaacagtctt gatttttggc ggtagtcaag gagcacttaa
    31381 aatcaatcaa gcagtatcag tttttttaag tgaatatatt ccttcttctt accaagtttt
    31441 gtatgcttca ggagatagat attatgaaga gatcctaaat aaaataaatc ctgaaataca
    31501 aaatattagt gtccagcctt atatcaaaaa tatggcagag gtcatggctt ccagtgatct
    31561 gctggtgggg cgtgctggtg caacttcgat cgcagaattc acagctctgg ggttaccggc
    31621 agtgttggtt cccagcccat atgttacgaa tgatcatcag actaaaaatg caatgagtct
    31681 tgttcacgca ggagcagcaa aaatgattgc agataatgaa ctaacaggtg aatcactaag
    31741 tcagacagtc aacgaaatca tgggtgatga agaattgcaa aagcaaatgt gccgagcttc
    31801 taaagaacaa ggaatccctg atgcttcaaa acgtctttat gacttagtaa aacaaataat
    31861 caaaaaataa aggcggtggt cttagtgagt aagaaaaaag aaccaaaaaa attgtctcat
    31921 ccgtcttctg atgaaaacaa tttgactcct tggcaaaagg ctaaccgaaa atatctggca
    31981 gaacaagaga tgaaaaacca agagccaaaa aaagaagaca taacattaga tacacccctt
    32041 tcagattctg aagaagaaaa aacggttcaa gaggaacagc aagaagaaag ccagtccgag
    32101 aaaaaaatcg aattttttga agagatcaag caaaagaaaa acggtggacc atataacggt
    32161 tcttttttga accggctgcc caatttaaaa agtcaacggc acaaagtact atttcgtcgg
    32221 ttagctttga tcatcactgt attgggtata ccgctgattt ttttgatcta ttatgtttct
    32281 ccttatagca aattacaagc agttgcagta tcaggcaata agacagtcaa cagccaagaa
    32341 atcatctctg atacaaaatt gtcattagga gaaaatgttt ggggacagta ttttcatcga
    32401 tctacttata tcgatcgcct taaaaaagca cagccaagaa tcgaaacagc aaatatacat
    32461 ttcaaaggga tgaatgaatt cgatttggat gtaacagaat ataaagagat tgctttgatc
    32521 gcaaaaaaca atcaatacta cccagtgatt gaaaatggaa cagtattaga tgaaaaagta
    32581 gcaaatccta caaaaaatct ccctatcttg gaagagttca aagactctgc aaaaatcaaa
    32641 gaattaacaa agcaatacaa tcagttatca tcagaattac aaaaagcaat ttctgaaatc
    32701 aagtacacgc caagagcaag caataaaaat ctgatccaat tgaatatgaa tgacggcaat
    32761 caagttattg taaatatcaa taatcttgcg aaccaaatga aatattattc gcaagtagcc
    32821 aaagatatgg atgaaaaagg tgtgatcgac atggaagttg gtatcttctc gcatccttac
    32881 ggtagcactc aagaaacgga agaatcgggc caatcggaag aaacaactga aagtgaagaa
    32941 aatgcggatc aaacaaacga atcaagtgaa gaggcagaaa cgcaaaaagc acaaacacct
    33001 ggatcgtcta gcacaaacgg atcatctgat tcaaatgatt caagtgaaac aagtcaaata
    33061 gaagataatc caagtacaac tccgtcaagt ttaaatgaaa attgataaaa caactttaaa
    33121 aagtaagaat gtactttcta aaaaccagta atttatggta gaattggaag cagtgaatat
    33181 atctattaat aagtatattt taaaacggac gctagttgtt cgcttgtata tggaatagta
    33241 agtaggaggg aatcccattc atggcaaaaa caggaatgta tgtaggcctt gatattggaa
    33301 cgacatctgt taaagttgtc gtggctgaat atatcgacag ccaaatgaat attattggag
    33361 taggaaacgc aaaatcagaa gggattaacc gaggcattat cgttgatatc gataaaacgg
    33421 tccaagcgat acaacgagca gtacgacaag cagaagaaaa agcaggtatc cagatcaaag
    33481 gcgtaagtgt cggtttgcca gcaaacatgc ttgaagtaga aaattgccag ggaatgattg
    33541 cagttaacgg agattcaaag gaaatcaccg atgaagatgt cagaaatgtg gcttcagctg
    33601 cccttgttcg ttcaatacct cctgaacgtc aaatcgtgtc aatattgcca caagatttta
    33661 ctgtcgatgg gttcgaggga atcaaagatc ctcgtggaat gatcggcgta agattagaaa
    33721 tgtacggtct gttgtttaca ggacctaaaa cgatcatcca taacatacgt aaatgcgtgg
    33781 aaaatgccgg attaatcgtc aacgaaatgg tgattactcc tttagcgctg actgaatcaa
    33841 ttctttctga cggagaaaaa gattttggta cgatcgtcat tgatatgggt ggcggccaaa
    33901 caacaacggc tgttatgcat gataagcaat tgaagtttac caatcttgac caagaaggtg
    33961 gcgaatttgt cactaaagat atctctattg tattaaatac gtcatttaac aatgcagaag
    34021 cgttgaaaat aaactacggg gatgcatacc cagaacggac atcaccagat gaagaatttc
    34081 cagtagatgt gattggtcag tcagaaccag tgaaagtcga tgaacgctac ttgtcagaaa
    34141 ttatttcagc tcgaatggaa cagattttca acaaagcaaa agatgcattg gaccaaatcg
    34201 aagcattaga gttgccaggt ggcgtcgtat tgacaggcgg agctgctagt cttcctggtg
    34261 ttgtggactt ggcacaagaa atcttcggcg taaatgtaaa attgtatgtt ccaaatcaca
    34321 tggggctgag aaacccggta tttacaaatg taataagtat cgtggattat tcagcaaact
    34381 tgagtgaagt gtatcaattg gcaaaaattg ctgttacggg tgaaacctca gcagctcgtc
    34441 aaatggtagt tgaacaagaa acaacaaaca catatgagag ctatgaagca cctgaagaaa
    34501 caatctacga tgagccggaa ccaaaagagt ccggagaaaa tgtaaaaaat aaaatcaagg
    34561 gtttctttac aaatattttt gattaattca tcctgggagg aataagatat ggaattctca
    34621 attgataata atatcaatga cggcgcagta atcaaagtca tcggagtcgg tggtggcggt
    34681 ggaaatgctg taaaccgaat gattgaagaa aacgttaaag gtgttgaatt cattacagcc
    34741 aacacagacg tgcaagcatt aaaaaattca aaagctgaaa cagttatcca attaggcccc
    34801 aaatatactc gtggtttagg tgctggttct cagccagaag ttggacaaaa agcggcagaa
    34861 gaaagtgaac aatccttacg cgaagcttta gacggtgcag atatgatttt catcactgca
    34921 ggaatgggtg gaggaaccgg tacaggtgct gcaccaatcg ttgcaggtat cgcgaaagaa
    34981 ttgggcgcat tgactgttgg tgtcgtgaca cgtccattca cttttgaagg accgaaacgt
    35041 ggccgttttg ctgcagaagg tatcgctcgt ttgaaagaaa atgtggatac attattaatt
    35101 atctcaaata accgtttatt agaagttgta gataaaaaga caccgatgct tgaagctttc
    35161 cgtgaagcag ataatgtttt acgacaaggt gtacaaggta tctctgattt gatcacagcg
    35221 ccaggttacg ttaaccttga ctttgctgac gtgaaaacag tgatggaaaa ccaaggtaca
    35281 gcattgatgg ggatcggtgt ggctagtgga gaagatcgcg tagtagaagc aacgaaaaaa
    35341 gcaatctcct ctccattgct tgaaacatct attgatggag ctgaacaagt acttcttaac
    35401 atcacaggcg gtttggatat gacattgttc gaagcacaag atgcttcaga tatcgtagca
    35461 aatgcagcaa ctggcgatgt aaatatcatt ctaggtacat ctatcaatga agaaatgggc
    35521 gatgaaatcc gtgtgacggt catcgcaaca ggtatcgatg aatcaaaaaa ggaacgcaag
    35581 tctagccgac cagctagaca agcacaaatg caatcacctg ctcaaaagac tgtattagat
    35641 atggatcaag ctaaaccaac ttcttcagaa gaagaaaaca gttttggcga ttgggatatc
    35701 agacgtgaac aaaatgttcg tcctagagtt gatgattcca attttgacaa tatcgaaaaa
    35761 aaagaatttg atacattcaa ccgcgaagaa acaaaatctc atggcgatga tgaattaaat
    35821 acgccaccgt ttttccgtcg taaaaaataa aggagaggaa gagcacatga ttgctgacaa
    35881 cttgcagaaa atcaagcaag aaattcagga ttcgtgtgct cttgttcatc gtgatccgaa
    35941 agaagttaca ttagtagctg taacaaaatc tgttgaatcc gctcaagctg aagaattggt
    36001 tagagctggc gttatgaatt gtgcagaaaa ccgagtagat aaattattag aaaagaaaca
    36061 agatttattt gattttcctg aaataaagtg gcatttaatt ggtaatttac agcgcagaaa
    36121 ggtaaaattg attgtaaatg aaattgatta ctttcatgcg ttggacagtt taagattagc
    36181 agaagaaatc cagaagcggt tagagaaaga attatcctgt tttattgaag taaatgtttc
    36241 tggtgaggaa tcaaaacatg gaatttcacc agatgagctt ttaccgtttg tggaatcttt
    36301 agctgcctgc acaaaaatca aggttgttgg gttgatgact atggcgccta aagaagcttc
    36361 tgatgagcaa atccataaag tgtttggtac gctgaaagaa ttgcgtgatc ttgttcaagc
    36421 aaaaggttat gcccatgcac catgtagtga attaagtatg gggatgagtc aagactttca
    36481 aattgcgatt gaagaaggtg caacatttat ccgtgtagga acagcgctat tcaaagacga
    36541 ataaaggagg aacaaaaatg ccgcttttaa acagaggagc aattgctaat tttttcggat
    36601 tagctgatga ggaagagtac gaataccaag attatcctga aacaacaaaa aaggctgtac
    36661 agccaactaa aaaaaataca acacagaatc aatcaactca ttctgtatct gctgcgaata
    36721 ctcatgaaaa acaaaaaacg gcgacttcac gtcctgtaac gcatgaaagt attcagccgg
    36781 ctgaaaagaa aaaagtaatt gaactacatc aatcacctac aaaaactgta gcgaaagaaa
    36841 gaacagcttc aagaacctct acacaaccta aagcaaatct tgctggaaaa atcactgtaa
    36901 tggaaccaag agcttattct gaagctatgg cgattgctaa acgaatcatt gctggagaag
    36961 caaccttagt gaattttcat ttagtagaag aaaaacaagc gagaagaatc gttgattttc
    37021 taacaggaac agtttatgca ctagatggag atatcaaacg tgttggagat gagatttttc
    37081 tttgtacacc gactgggatg gagattgaca gttcaaccgc tcaatctttt gcagatgcga
    37141 atttgtttga tctgtaagag gaggaactac gatttatact attgcactat tacttcattt
    37201 acttcaacgg gcagcctatt tctatagcat tatcttgatg gtatatgcat tgctctcttg
    37261 gtttccaggc gggtatcaat cagggctagg acgattatta gctaagatct gcgaaccgta
    37321 tatcagtttg ttcgatcgtc tgcctttgag gattggaccc ttggatttca caattttagg
    37381 ggcaatcctg atcttgaact tagccgtaca aggtttagca cagatcgtta tatggataac
    37441 tgtccattta gtttattgat tgaagcaaca aaatctttat tctcagaata gataggaggg
    37501 gaaaattttg gatgcaaatg tataccagca ctttcgggca gatgaacgta catttatcga
    37561 ttcggtaggc gattggattg aacaggttca aggacaatat gcaccctatc tgaccgaatt
    37621 tcttgaccca agacaatcct atattttaga aacactgatt cgacaaagca gtgatttgcg
    37681 tttcatgttt tatggcggat atgaacaggc agaaagaaaa agatgtttga ttttccccga
    37741 ttattacgaa cctgtagagg aagattttga aacagcattg tatgaaatcc attatcctag
    37801 caaatttgcc agcctaagtc atgggaaaat ccttggaact ttagtgggaa cggggatcaa
    37861 acgctcttac tttggcgata tcatctcgga cggtgatcgc tggcaagtat ttattgcaaa
    37921 tgaggtggat cattttgttg ctgcacaagt aacaaagatt ggaaaggttg ctgtacgctt
    37981 ggaaccaatc aaatacacag aaatgattat gccgaaagat agctggacac aagaaagagg
    38041 aacagttagt tccatgcgac tagacagtgt gatctctgag atttataata tttccagaca
    38101 gcggtccaag cagctgatcg aagctggtaa agtaaaagtg aactggacag aaaatacaag
    38161 acctgatttt gtacttgaac tattagatat tatttcagtc agaggttatg gcagagtcca
    38221 gatccaagaa cttgagggga agacgaaaaa agaaaagtac agggtactgt tcggtgtatt
    38281 acgtaaataa aataaaaagg tggtttactc atggctttaa ctccattaga tatccaaaat
    38341 aagacattcc cgacaaaaat gcggggatat aatcaagacg aagtagatga tttcttagat
    38401 ttagttgttc gtgactatga ggaattgact caaagaaatc gagaattaga aaaagcagtc
    38461 aaacattctg aagaaaaact tgagtacttc aatgaattaa aagatgcttt gaaccaatca
    38521 atcatcgtag ctcaagatac ggctgataaa gtaaaaacaa gtgcaagcaa ggaatccgaa
    38581 gtgatcgtta cttctgcaca aaacaaagca gatgagttag ttgcgaacgc tgaaaaacgt
    38641 gcacatcaat taacaactga tgcagaagaa aaagcaagaa aaatcttgac agatgcaaca
    38701 gaaaaagcaa gacagcttgc gacagaaact gaagatctta agaaaaaaac acgagtattc
    38761 catcaacgta tcagcttgat gcttgagtct caattagaac aagtgaaaag tccggaatgg
    38821 gatgaaattt tacaaccatt ctccagctac gtgactgaca gccatgaagt gatcaaagaa
    38881 gttttagcaa aacagcttga caatgaaaat gatactgatg taaactcaga cacaggagtc
    38941 atagaagcac ctatgacggc atttgataca caggctattg atatctcgat cgtgcctggt
    39001 gaaaatgaaa acgagtagaa cagaaaagca ctttgttatt cttaaagcga gccgatgaga
    39061 gtggaagttc ggtaagacac tgcatggtga aagatcctct acttgagacc tgcttttccg
    39121 aaattagagt aagaagcagc ggctgacctc gttattggtc acagagagtt tgcactagat
    39181 agatgcaact aaatttgggt ggtaccacgg catgttcgtc ccttgcggct gaacatgctt
    39241 ttttgtgtcc ggaaaacaaa taaaggagtg tatggttgaa tgaaaatgaa agaaaccttg
    39301 caattaggaa aaacaaaatt ccctatgcgt ggtaatttgc caaaccgaga aggcgagtgg
    39361 caaaaagaat gggaagaaaa tcgaatctac caaaaaagac aagagttgaa cgaaggaaaa
    39421 cctagctttg ttcttcatga tggacctcca tatgccaatg gtaatatcca tttaggacat
    39481 tcattgaata aaatcagtaa agacattatc attcgttcaa aatcaatgtc tggctatcgt
    39541 gcaccatatg ttccaggatg ggatacacat ggattgccga ttgaacaagt attggctaat
    39601 aaaggcgtga aacgtaaaga aatgtccatg gcagaatact tggaaaaatg tcgtgaatat
    39661 gcactttccc aagtagataa gcaacgagca gatttcaaac gcctaggagt tgcaggagat
    39721 tgggaacatc cttatgttac attagatcct tcttatgaag ctgcacaagt acgcgtattt
    39781 ggtaaaatgg ctgaaaaagg ctatatttac aaaggattga aaccaattta ctggtctccg
    39841 tcaagtgagt cttctttagc ggaagctgaa attgaatata aagatgtaaa atctccatct
    39901 atttttgtag ctttcaatgt caaagacggc aaagatattc ttgatgaaga tacagctttt
    39961 gtcatttgga cgacaacacc atggacattg ccagcaaacc aaggtattgc tgtaaatcca
    40021 aaatttacgt atgtattggt ggaagccgat ggccgtaagt ttgtcgtagc gaaagatttg
    40081 ctagaaacag tccaatcaga aatcggttgg gaaaatgtaa cagttctaaa agaatttgct
    40141 ggacaagaaa tggaatatat gactgcaagt catccattct atgaacgtga atcacttgtg
    40201 attttaggcg atcatgttac tttagacgct ggtactggat tagtccatac tgctccagga
    40261 catggggaag atgactatat cgctggtagc aaatataaac tatctgtagt gtcacctgta
    40321 gactcaaaag gattgtttac agaagaagca cctggatttg aaggtatctt ttatgacaaa
    40381 gcaaatccta tgatcactga tctattaaaa gaaaaaggag ctttgctgaa gctagatttc
    40441 tttactcata gttatcctca cgattggcgg acgaaaaagc ctgtgatcta tcgggcaact
    40501 ccacaatggt ttgcatctat cgataaattc cgtcagaata ttttggatga agtagaaaaa
    40561 gtagagtggg ttatcccatg gggtaaaaca cgtttgtata atatgatccg tgaccgcggc
    40621 gattgggtaa tttcccgtca gcgtgcatgg ggagtaccac ttccgatttt ctatgctgaa
    40681 aatggagaag caatcatcac accagaaaca atcgaacatg tggcaaaatt atttgctgaa
    40741 catggatcaa ttatctggtt tgaaaaagaa gcgaaagaac ttcttcctga aggatttacc
    40801 cacccaggtt ctcctaatgg cgaatttaca aaagaaaaag atatcatgga tgtctggttt
    40861 gattctggtt cttctcatga agctgtttta cgtcagcgac cagaattatc attcccagca
    40921 gatatgtatt tagaaggatc agaccaatat cgtggctggt tcaactcaag tatcacgact
    40981 agtgtggcta tcaatggtgt agcaccatac aaatcagtta tttctcaagg atttgcttta
    41041 gatggtgaag gccgtaagat gagtaaatct ttaggaaaca tcattgcacc agaaaaagtg
    41101 atcaaacaat ttggtgcaga tatccttcgc ttatgggtat ctagtgtaga ttatgaagca
    41161 gatgttcgtg tttctatgga tatcttaagt caagtggcag aagtatatcg taaaatcaga
    41221 aatacgatgc gtttcttatt agcgaataca gaagatttcg atcctaaaaa agatactgtt
    41281 tcgtacaatg atttacgttc tgtagacaaa tatatgaccg ttcgattgaa ccaagtaatc
    41341 aaagaaatcc gagaagaagg gtacgacaaa tataacttca tgcatattta ccgtacagtt
    41401 atgaacttct tgacggttga tctatcgtca ttctacctgg attttgctaa agatgtagtt
    41461 tatattgaag cagaagatga ttatcaacgt cgttgcatgc aaactgtttt ctatcaaaca
    41521 gctgtagctt tgacaaaatt attgactcct atcattccgc atacagcaga agaaatctgg
    41581 tcattcctga aagaagaaga ggagtacgtt cagttaagcg agtttcctgg ttatgaagag
    41641 tttgcaaacc aagaagaatt gatggatacc tggactgctt tcatggactt ccgtgataat
    41701 gtcttaaaag cattggaaga agcaagaaac agcaaattga ttggtaaatc tttggaagca
    41761 aaactaactg tttatccaaa acaacaagtt cgtgaaatgt taaaagcttt ggatgcagat
    41821 attgcacaat tattgatcgt ttcaccagac tactttgaag ttatggcagc agatgaacaa
    41881 gtgccagata acgctatggt atttgatgat gttgctatta cagtggaaaa agcagacggg
    41941 gaaacttgcg atcgctgtcg ccaagttcgt aaagatgtag gagtagatga aaaacttcct
    42001 catctatgcg gtcgttgtgc aaaaatcgtt gaagacttct atcctgaagc tgtagctgaa
    42061 ggattcgaag aaaagtaaaa gggaaaaggg ggtgtgacat gttctttgtc acaccccctt
    42121 ttgttatcaa tcgtttaatt ttccacgttc acgataccag ttatctggtt gccagatcca
    42181 ttcgaatccg tctttttcaa gtagttcgaa agctgcttta ggtcccattg atccagcttt
    42241 gtagttggga aaatccggtt gtgtttggtc ccaagctttt cgaatgacat ccacgatatg
    42301 ccaagattga gcgacttctt cccaatgaga gaagttcgtt ccatcaccat tcaacgcatc
    42361 taatagtaat ttttcgtaag cttctgggct attttcaacc atttctgcac tatttctaaa
    42421 ttccaatttg attggttccg tttcaaatcc ttgccctact tctttcccat tcaaggataa
    42481 agaaaatcct tcagttggct gaatatagat cgttagaaca ttaggtggaa gtgtcgtatc
    42541 gtcacatggt tcatcgacag atgttttgaa tacattaaca ggaacttgtt tgaatacgat
    42601 gttgatccga gtcccttttt cagtcaaacg tttgcctgta cgtacataga aaggaacacc
    42661 tgaccaacgg aagttatcga tagtgaattt gcctgcgacg aatgtttcag tctctgattg
    42721 agcatccact ttgtcttctt cgcgatagcc cttgaattgt acaccatcca attcaccttg
    42781 agcatattgc ccgcgaacga agttttctaa cacttcttct tcgttataaa tgcggatagc
    42841 tttcaatgct ttgatctttt ctgtacggat ttctttttcg gaaaatgcta ctggcggttc
    42901 cattgcaagt aatgctacaa cctgtaaaat atgattttgc accatgtctt taagtgcgcc
    42961 actatgatcg tagtagcccc cgcgatcttc tacaccgagg ttttcgccaa aagtaatttg
    43021 gatattgtcg atatagcgat tgttccattg agcctcaaaa atattattgg caaaacggat
    43081 agctgagatg ttttggatca tttcttttcc taaataatga tcaatcctga aaatatcttg
    43141 ttctgggaat acttctctga tttgctggtt caattcaaaa gcagactggt aatcagaacc
    43201 gaacggtttt tctatgatca aacgattgaa gccatttttt gtcataatat tttgtgattt
    43261 caaatgagaa acgatcgttc caaaaaattg aggtgccata gccaaataat agacacggtt
    43321 accgtttaat tgatagtgtc catctaatcg gtcagacaat tcttttaatg tctgataatg
    43381 cgttgaatca tttacatcat gggattgata gtaaaaatgg cttgcaaagg tagttgcttc
    43441 ttcatctgaa ggattcaatg cacggattgt ttctttgaca acttcacggt agtgctcatc
    43501 gctccaggga cggcgagcgg ttccgattac agcaaaatgc tcgcctaaat ttccttttct
    43561 gtaaaggcgg aacaaagacg ggtacaattt acgctgggct aggtcacctg taccgccgaa
    43621 tatcgtaaac aatacatttt tttcattaga catgtggata cactccttta tattttatcg
    43681 acaaaaatcg tgctggccgc cttaaagctg acggggattt tctttctatc attttggata
    43741 gtgatcggtc cttcataagc tgcgatgtca acgatctcat attcttcgtt gatattcagg
    43801 tcaatagtga ctaagtaatc caacaattct ttttcatcaa gcactcgtgc gatacgcact
    43861 tttgtaccga caggatagga ctccaatgtt tgtcggcgtt cttcatggac aacttcctct
    43921 tttttaggaa tcgatcctcc atgaggacaa tgttcaggat gattcaaata gttttctaaa
    43981 tgatctgcaa gatgttccga agtcacatgt tccaatactt cagcatcatc atggacttca
    44041 ttccagctat agtctaaatg ttcaactaaa aaaacttccc ataagcgatg tttgcgaatc
    44101 agactgctag ctttttgcag tccgcttgca gtcaattgta caccttgata aggggaatgt
    44161 tctacaagct cttctttcac aagtttagaa atcatttcac tgacagatgc agctgagaca
    44221 tctagactag atacgatttg tttattgttg actttcgttg tatctccacc tagttctaaa
    44281 atgattttca aataatcttc tcgatttggt gtcatattcg atcatccttt catggtaaaa
    44341 aaccatcatt agtaagtgta acaaaaaaaa cgttaattga ctatttttct aaataaaaaa
    44401 caaaggattt ttagacgttt ttacaacaaa aaacgaaaga tgatagagaa gactcattct
    44461 ttcgtttata aaggatatat atatacattg ggagggatct atttttcaga taacaactta
    44521 tatgaagtgt ctgcagattc gtcataaaga gtttgttcgg gataagaaaa gataagataa
    44581 aaaccatact gatcacgtac gactaaacga tattcatcta tttctaccac agatgtgcca
    44641 agagactgac cgttgatcaa aaacgtgtaa tcagatgtaa tctgtaattg ttgtttttgc
    44701 tggctatcac taaagaacca tgtaccttga tactttgaga gtccttctga cttttccgct
    44761 tgttttttct ttttcaagta aataaaactt gagagaacgg taatgagtga accaagaaaa
    44821 tagatcattt tttttatcac caaatcactc ctttatgaga aaataatcgc atagacatat
    44881 tctttcttct ttatttcatt gtaccgaata aaagctaaaa tttctctaag actttcgact
    44941 ggttttttta ctgtttttct acaaattatg ataggataca tagtaggaag aaaggtgaca
    45001 taatttgaag tatcagcaag tggaaatagc tttttttatt gaccgcccga atcggtttat
    45061 agcacattgt atgaaaaaag acggagaaat aattattgcg catgtcaaaa atacaggtag
    45121 aggaaaagag gtcctgatac caggcgctga agtggctatt gtatttgctc ctggacctaa
    45181 acggaaaacc gcctatgatc tgattgctgt aaaaaagaag ggtgactggt ttaatatcga
    45241 tagtcaattg cctaatcggc tggcaattga tggtatttta gacggcacga tccttttacc
    45301 aggattaact ggtccattta cggtatacaa gcgtgaagta acttatcttc actccaaatt
    45361 tgatatttat ttagagacgg tggtcggaca aaaagcattt gttgaagtca aaggtatgac
    45421 acttgaaaat aaagcgattg gtgcttttcc tgatgcgccc acactcagag gattgaaaca
    45481 tataggagaa ttaacgtatg ccgcacaaga tggttatgcc gcttatgtct tgtttattgc
    45541 tcagttcgaa catcttcatc tggcaacgat ccatgaagaa atgcaaccag cactagctga
    45601 tatggttcga catgcgcagc aaagcggagt tcaaatcctc gcctataatt gtcaagtatc
    45661 ggcagatgag gtaactgtca agcaggccat tccatttgat gtagatatcc cttttgaaga
    45721 tcctatacaa tagaaagaag gtaaaataat gatcaactta ggtattatcg gaacaaattg
    45781 gattactcat caatttgtcc aagcagcttt aagcacaaaa aaatatgaat tgacagcggt
    45841 atattctaga catttggaaa aagcacagga atttggttct cattatgaag gggatatcga
    45901 attcgcaaca gatttgaaga cttttttcaa tattactcat atggacacca tttatattgc
    45961 ttctccgaat agtcttcact ttagtcaagc taagcaagca attttggcag gaaaaaatgt
    46021 gatagttgaa aagccagctt tttcgacgcc aaaagaaatg gatgaaatca tccatttggc
    46081 aaatgaacac aatgtgtatt tttttgaagc agcaagaaat atccatgaat caggttttaa
    46141 aaagattgcc gattacttac cattaaaaga cgagataatc ggtgcaaatt tcagctatat
    46201 gaaatattct tcgcgctatg accaagtatt agatggagaa gaaccgaata tcttctcgac
    46261 tcatttctca ggtggtgcat tgatggattt aggtgtttac cttgtatatg cagctgttgc
    46321 ttggtttgga atgccaaaag aagtccatta tttcccaaga aagatttcta ctggtgtaga
    46381 tggtcttgga tgggggatct tacgttatga gaattttgat gtatcgatcc agccaggtaa
    46441 aatcggtgat tcttatttac cgtcagagat ttattttgac tcaggaacgc tagtgatgaa
    46501 tggagtcaat gcaattgaac aagcacaatt tcatgatcgc gcgcataaag aaatcattga
    46561 tttggcggta acagctgcag aaaatccaat ggcagatgaa gcagaggatt ttgccaatgt
    46621 catggctcat ccgaaagatc cagcttgggg attgttgtat gaagagtgga tcgagctagc
    46681 tagaaatgtg aaccaagtga tttatgattt aagaaaaaat ggcggcatca aattcgatgc
    46741 agataatgag gaagattttt aatgaacgaa tttgtacaag tatttcccga aaattggcag
    46801 aaaaaatatg aggataaagg gtttatctca ccttctctta ttcaacgagc agtttttcag
    46861 ccactaagta ataaacaaag tatagtagcg gtttctccta caggttcagg taaaacactg
    46921 gcttatcttt ggcctctact actaaacgtg gaaccaggtg aagcaagtgc gttggtcatt
    46981 tttgcttctt ctcaagaact agctattcaa gtagcagatg ttgcacgtga atggggaaaa
    47041 gacaaagagt tgaaagtcca atctttggtg ggcggagcca acgtcaaacg tcagattgag
    47101 ggtttgaagg aaaaaccaga aatcttgatt ggtacaccag gaagaatatt ggaattgatg
    47161 aaagcaaaaa aaataaaagc acatcaagtg aaaacaatgg tatttgatga agcagaccag
    47221 ctatttgacg ctgggaacag ccaaatcatt gatcaaattc ttcatcaagc tccaacagag
    47281 tatcagctag cttttttttc agcgacagca gatcgttccc ttgaaagcat tgaaaaaatc
    47341 actgggaaaa cgtttgagac aattgacgtg acagcagagg acgattctcg aaaaggactg
    47401 agacattact ttttacgtgt accgatacga aagaaagatg actacttgag acgtctcacg
    47461 catatagaag attttcatgg tctgattttc tttaatcaac tgactgaatt aggcgtaatg
    47521 gaagaaaaat tgctgtatag aggtgtaccg gtaggaagtc tggcctctga tcagaacaag
    47581 ctactccgaa aagcagcgct agaacaattc aaaaagaaaa tggtcagagc acttttgacc
    47641 acagatgtgg cggctagagg tttagatatt acaggtcttc cttatgtagt gaatgcagaa
    47701 gttccgttaa gtgaagaagc ttatttgcat cgttcgggaa gaactggtcg aatgggtaat
    47761 gaaggaatag taattacact cgtacaagat aataatctaa gagatttaaa acggctttta
    47821 cgtccatcaa atataacact tgaagagatt tttttatacg gaggtaagct gcaaactgag
    47881 cagccaacca aagaagatgc agctcctgca gtaaataaaa agtatgcgaa aactttttct
    47941 ggaaaggagc agaacccaga atataacaaa gaaaaaataa gaaaaaaagt taaaaataaa
    48001 tcaaaaaaag agagaaataa aggagcaagg cgcaaataag cgccttttta tgctatgatt
    48061 atgaaaacgg gaacaaaatt ggttgccaga ggaggggtta tgtgaaagaa cgtcaagaag
    48121 agatcttgca gctattgaca gaaaaccaac aaggattgac agcaagtgat gtagctgagc
    48181 gcttaactat cgatcgaagc aatgcaagcc gatatttaag tgaactatac aaggcccatc
    48241 atattgtaaa aaccgctggc cgaccagtcg tttactcttt gccaacggaa aagtcaaaat
    48301 ctgatgaagt gcatgtcgac tcttctacgc aagtgacttt tgaaacactc gttggagaaa
    48361 atgattcttt aaaagtcagc atacagcagg caaaagctgc gattctttat ccaccgcggg
    48421 ggttgcatac gattattttt ggagaaactg gtacagggaa gtcgatgttt gctgaatgta
    48481 tgtatcattt tgcaatcgat tcagagatgc tttctgcaga tgcgccattt gtttctttca
    48541 attgtgcgga ttatgctcaa aatccgcaac tgctctttgg acacatcttt gggatcaaaa
    48601 aaggggctta cacaggagca gctcaagata gtcccggtct aatagccaaa gcagacggcg
    48661 gtatattgtt tttagatgag atccaccgtt taccgccgga agggcaggaa atgctgttta
    48721 ctttcattga taaaggtatt taccgtccgc ttggagaaag tgcacaagta catgaagcat
    48781 ctgttcagat cattggggcg acgactgaat cttcagaaag tttcttatct acgttcaatc
    48841 gacggattcc tatggcaatc accttgccga atcttgcttc acgttcactg gatgagcgtt
    48901 atgaaatcat ctctcttttt atcaaacaag aagcgaatcg tttgaatcaa cgtatcgata
    48961 tcgaaaaaga agcgattctt gccttcatgc tttatgatgc ggaaggaaat atcggtcaag
    49021 tgaaacgaga cttgaaacta gtctgtgcaa aatctttttt acattaccgt actcatcatg
    49081 agaaaaagct aatcattcga aaagaagaac tttcccttca agtacaaaaa ggattactga
    49141 aaatcaagga aataccggaa cgtctcgacc gattcatgga cagcaaaagt cagtatttga
    49201 catttgaacc aggatttgct gatgttgtct ggtcacaaga tccagaacga aacatgcagg
    49261 tctataacga tattgaggaa aaagtcactt ctctttccga aaccggtgtg gagaacattg
    49321 atctagagac cctcatttca aaagatgtgg atgcttattt ccagacatat gtcaaagaat
    49381 tgactaaaag ttcgattccc aaagatcttt tgccagatga aatctggcgg ttaaccaatc
    49441 aattgtatga tgtggctgaa aaacggttag accgtattta taatgaaaaa gctcgttttg
    49501 cttttgcttt gcatctgcaa agtacattgg atcgtgtgag agaaggtcat atgatcgtcc
    49561 acccagattt gaataacgta cggaaaaatt ttaagaggga attccaggta gcaatcgatc
    49621 tttcaacgat cattgaagaa gaagaacaag ttgagattcc ttttgatgaa atcggcttta
    49681 ttagtatgtt tctttctatc aatctaggag aaagtgagcc tgttaaagaa aacaaagtag
    49741 atgttctcat attgatgcac ggcagacaga ctgcttcaag catgttggag atggcacaag
    49801 aactattagg aacaaaagta gggaaagctt ttaatatgcc actggttatg gaagttcaaa
    49861 caatgtatga agaagtgtta caatatgtca aaaactatca agagcatttg acgaatggcc
    49921 tactcttgtt aacagatatg ggatcattaa acaccttcag tgatttgatt tatgaagaga
    49981 ctggcattcg gacgaaagct atcacgatga caagtaccat gattgtgata gaagcgatac
    50041 ggatggcaag tgttggaaga agcttagaag atatttatca aaatatccag ctttcctttg
    50101 aatcgatcgt acgtgatcag ttcaaagcgg aaaagtctga aactttacag cgtaaaaaag
    50161 ccgtaatcgt gacatgcttt actggtgaag gagtagctgc caaattatat cagcgtatta
    50221 tgccagtgat taatcaaaat aaggtagaac tgatccagat gcagttttta gaacgtgaat
    50281 cattcaaaaa acacatcgat gccttattgg aagaatatga gatcaaagca attgcgggaa
    50341 cagttgatgt tgattatcaa aatatcccat ttttttcagc ctatgacgtt tttgatgatg
    50401 aaaaactaaa tattttaaag cgtatagctg atgatgagat cccgacagct aaaattgtcg
    50461 attcacttgc tggatcatta agcgatgtct tttctattga tgaactgatc cgttcattgc
    50521 aaaagacagt tcagcagatc cagaccgatt tgtcgattat cgtggaacct ggagtagata
    50581 ctggaatcgt tatccattta gccttcatgt tagacggatt gatcaaacaa gaaagccatc
    50641 ggcaatttga gaaactgtct attttctcaa aaatttatcg tttggaaatg gatatcgttc
    50701 gggcaaactt gatggcacta gaacgaaaat atcaggtaaa aattccagaa gatgatatcg
    50761 catttcttac acaaatgttt cttgaaaata aaataaaaaa atgaacacac taatcatact
    50821 gtttacacac taaacatctg tgtgcgcact ataaaaaaga gctaaaaagc atgaaaacgc
    50881 tatttcagct ctttttttta ttggcatgct tcttgcgtta tataaaagtg taaaaaaata
    50941 aaaggagtgt taaaaatggc aaaaaaaaca atcatgttag tttgttcagc agggatgagt
    51001 acgagtttat tggtcacaaa aatgcaaaaa gcagcagaag aaaaagggat ggaagccgat
    51061 atttttgcgg tctctgcctc agatgcagat aataatttag aatctaagaa tgtcgatgta
    51121 ttattattag gtcctcaagt acgtttcatg aaagctcaat ttgaacaaaa acttgcgcct
    51181 aaaggtattc cattagatgt catcaatatg caagactatg gcatgatgaa cggagaaaaa
    51241 gttcttgcac aagcagaaaa tctaatgaaa taaatcaagt atgcagttgg gggtataaca
    51301 acgtggaaga tcaacaaaat ctagaagcca tcatggggtt aatcatgttt ggcggtaatg
    51361 caaagagcga tgcaatggaa gcgattgcgg ctgctaaaat cggtgatttt gagttagcgg
    51421 ataggaaaat cgctgatgca gaagaatcac ttatacaagc gcatcatgca caaacagaca
    51481 tgttgacaca agaagcacaa ggaaatcata tgcaagtgac attattgact gttcacagtc
    51541 aagatcactt aatgacatct attgcgttta ctgatttagc aaaagaaatc atcgaacttt
    51601 atcgtcgcgt atacagcgaa taaatacaac ttcttttttg aacggaagaa gaaaaatttt
    51661 tgaacggatt ttttattttg ttataaggag aactctcttt atataaaaaa cttataggag
    51721 gaagtaaaaa atgaacggac tatcaagttg gatggagaaa tacatcctgc cggttgccgc
    51781 aaaaatcgga gcgcaaaaac atttagttgc tttgcgtgat gcttttatcg gcacactgcc
    51841 cgcaacaatg gcgggatcag tcgctgtaat gatcaatgca attattcgtg atttaccgca
    51901 acagtttaac agtacctatg ctgacagttt agcagcagac aaaggaatct ttgccgtatt
    51961 gaatgtcatc atcggaatca atggttttgt ctggaacggt acactagcaa tcgccggatt
    52021 gatctttgcc ttttcttggg ggtataatct tgcccgagct tatggagtaa atgatctagc
    52081 tggggggatc gttggtttag ctaccttgat tcaaggtatt tcctttggtc cagcattatc
    52141 agcagcatta gatgtgaatg ttccttcaaa tgtcgcagat gcaatcaata gttctgagat
    52201 tggtgcaacg attgcagatg gcacactgtc agttggatta tggggttggc taaaactaga
    52261 tcatttgaat ggaaacgctt attttacagt tatgatcatg ggagctattt cagtcattat
    52321 tttttgtaaa ttgatgcttg ccaacattac gatcaaaatg cctgattcag taccaccagc
    52381 agtatctaaa gcatttgcag ccattttacc agcgacgatc gctttatacg tcattgctat
    52441 tatcaattat atcgtaggtc gtgtagctga tgggcaactg atcattgatt tggtgcaaaa
    52501 atatattgca gagccattcc taggattatc tcaagggttg ggagcagtat tgatcgtcac
    52561 agtatttgtt caagtattct ggttctttgg tattcatgga ccaaatgtat tggcaccagt
    52621 gctagaggga atctggggac aagcacaact aatcaatatt gatattttcc aaaaaggtta
    52681 taacggaaaa actggtacac cagccgtgtt agatgcaatc gaagatggaa aagcttatat
    52741 gtgggtccgc ggatcgttcg atgcttttgc atggtttggc ggatcaggcg gaacgatcgt
    52801 cttactgata gctatcttga ttttctctaa acgtgcagat tatctaacag tagctaaatt
    52861 atccattggg cctggtatct tcaatatcaa cgaaccagtg atgtttggtc tgccaatcgt
    52921 attgaatgca atcatgttta tcccattctt gattgctcca gtagtagcaa cagcaattgg
    52981 atggacagct acttatcttg gcctagtcgc tccggtatcg cagcaagtca catgggtagt
    53041 accgccgttc ttattgtcat tcttagcaac aggagcggat tggcgggcac cgatcgtaac
    53101 attagtatgt atggtcgtga ctttcttgat ttgggcacca tttgtcatct ctgcaaacaa
    53161 aatggaccct gccgcaggcg aacaacaata agatgaacgt gaggagcggg acgctttaag
    53221 tttcgttcct cataatgaat tttcgaggag gaactgaaat gattacaatt gccagtgcaa
    53281 tgcaacaaga tgaaatcaaa gaattgttag aaggatacaa tgaagaagga atgactttta
    53341 catttaaaga aaaacaagga atcaagctgt tctttgaaac aagtgcagaa gatccggaag
    53401 ttgcggctaa aaaagcaaag gaactaatca aagcacagcc ttggggaaca gtcctttatt
    53461 ttcaatcaac agctgtttag aaaatggttt aaaaagatag gatggttgag atgataaaaa
    53521 acggtttatt tttatgtagt attggttctt tgatcgtgtt gtatagtttg aatccacatt
    53581 cgcaaaggta tgatttgtat agtatggtca caggtctttt tctaacagga ttaggaatct
    53641 atttcttcat gaaggggaaa aaaagagaag aagcaaagga gaaaaataac taatggaaat
    53701 cacgaaaaaa ttaaatattc cagcatcgtt tttttacgat aaagttatgg actctgtcct
    53761 tttcgatgta cgtaaagcga ccggcaaatc agtaactaga aaacagctga aaaatttgga
    53821 gtacgtcaaa caattctcgc aaaataaccg ggctcgcata aaaatcgaag aagtcattga
    53881 aaaccagtct tataagtttc gtacatcgac aacgaagaat gaattcgtcg ttcattatca
    53941 aatcaaacca ttggacgatt cgacctgtga ggttcattac acagaaaaaa tggagtctta
    54001 tggcatgctt caaaagttga atgatatgct tttaggaaca atcttgatgt attttaaacg
    54061 gagacgtttc aaaaaaatgc tagatatgat cgaagcaacg tattaaatca atgaaacatg
    54121 gtactattta gttattgaaa aatgaagtaa gctagaagga atttttcata ggaagataga
    54181 tctttcctgc gaatcctcac tagctttttt gtaggaaggg gcagcaaaat gacaaacaaa
    54241 gtaagagagc taggtgtatc ttcgattgag gaaatgtttg atttagccac ttatgcattt
    54301 aatggggcag atacacctga aaggcgagaa cgttttcgta cacttgcgga gaattcttgg
    54361 aattacggat tttttgatga ggaggatcgg ctaacaagtc aagtcatggc gacccctttt
    54421 ccagtgaatt tttacggaca agagtaccta atggccggca ttggctatgt tgcttcttat
    54481 ccagaagcaa ggggacaagg cgggatcaat cagatcatgg aaaagatcct tgaagattgc
    54541 cgtgatcgta aagtaagctt atcttatctg gcgccattct cctatccctt ctatcggcgc
    54601 tatggctatg aacaatcatt tgataaaatc agttatcaac taaacagtcg tgatgtgccg
    54661 tttatcaaaa aaaaatctgg aaaaataaag cgtatggatt ttgaagatgc aaaagctgca
    54721 atcagaagaa tttatgaaca gatgccggaa aaccaacgag ctggtctgaa gcgcgaagac
    54781 tggtggtata cgtataaatt caagcaaaaa ggcaattacc agtttgcttt gcattttgat
    54841 gataatgagg aagcaacggg atatatcgtt tatcagcttg caacgtcttc ctttattatt
    54901 gcggagtggg gatttttgga tcatgatgct ttttgcagcc ttgttcgctt cgtaagttct
    54961 cacaatggtg cttttgaaac atttgaatat tcatgcggct acggcggtaa tgatcaaaat
    55021 tatctattag aaaatccatt tgcttctgta atgatcacac cttatatgat ggcgaggatc
    55081 gtagatattt ctttgttttt ggaaaattat ccatttaaaa agagacaagc tgcatttgcc
    55141 ctacaaatag aagaagatca ttatgcgaaa tggaatgaag gaatcgtgga agtaacaatc
    55201 gaaaatggaa aaaatcatct ctcaaaagta gaacaaacaa aactcccaat cgttcgagga
    55261 agtatccaac atatcacgca gctattatta gggtatcgta aagtggaaga gttggtattc
    55321 caagaaagaa tacaggtgac aaaagaatat gaagaaacgt taagctcaat cttgccacag
    55381 caacagccaa ttcttaatga ctatttttga taaaaaacgg gtgatgacaa tatttttgtc
    55441 accacccttt tgtttttcat ttggcatttg aaggaaaatg ttaaagtaaa tcctcttttt
    55501 tcgtaaattt gaattttttg ttttcttttg ttttagggtt ttcttttttg tggtcttgga
    55561 ttgcttcttt cgtaatcttt tcccgatttt ttttagatga atctaagcgt tctgcgtata
    55621 ctttttcttt tggcttatca ttcattggaa aggacctccc tacattattt cattctacct
    55681 ctagtttatc atctgttaag gactagtgga aacgttttgc ctaacttata agctgatttt
    55741 tttagcagat aagcttgcaa gtaaaaaaca aacaagctac aattgattta ttgaaattgt
    55801 tcctgtagct cagtaggata gagcaatcgc ctcctaagcg ataggtcgct ggttcaattc
    55861 cagtcaggaa cgtacaaata acccctgtat cctttgcggt acagggggtt attttgtata
    55921 agtagacatt gtagtttcta aactgctatg tcctaaacgt ttcgatacgc taagtatatt
    55981 tactccttga taaagtaaaa tagatgcatg cgtatgtctt agtccatcaa taaacagtcg
    56041 aagagaaaat agggaaaaat tgattactaa agaaaaactt actaggtcga cggtgtagtt
    56101 gattttcata cttcaataga ttcgaattaa acgatttcga ttggcttata ccagtcctat
    56161 ccaacgaaag atatatcgtt cttaactcta gcggtataac ttgaagcttt acgtaaagcc
    56221 aatgggaatg aactggagat gactttttat tatttttagt aatagctgat gggttaatac
    56281 ttacaacttt ccttttcaaa gtaccatata aaaaaacggt ggggttgttt agcgaaataa
    56341 aggatctaag ccaatagctt agatccttta ttctatgctt tatattattt tttgcatgtg
    56401 tgcgaaagta tactcaagcc caatcgccat ttcggaaaat cggtactgta gagccatctt
    56461 cacggattcc gtcgatatcc atcttgtcag atcctaccat gaaatctacg tgggtttgac
    56521 ttctattcaa tccagcttct gctaattctt catcggacat ttctgtaccg cctttcacac
    56581 tgaaggcata agcagaacct agtgctaagt gattcgatgc gttttcatca aataatgtat
    56641 tgaaaaagat gatgccagat tgagaaatag gtgaagggtc aggtacaagt gcgacttctc
    56701 ctaaacgacg cgcaccttca tctgtatcaa gcagttttgc taagacatct tctccttgtt
    56761 cggcagaaaa atcaacaact tttccatctt taaaagtaaa tttcataccc gagatgatgg
    56821 tgccagcata actcagtggc tttgtacttg aaatgtaacc atctactcga tgactgtcag
    56881 gtgctgtgaa cacttcttcc gtcggcatat ttgccatgaa tttttctcca cgagcattat
    56941 agcttccagc gccttcccaa agatggtttt taggcaatcc gataatgata tctgtaccgg
    57001 gagcagtata gtgaagggca gaaaattgtt ctcgattcaa ttcttctgct ttttttgcta
    57061 atttttcatc atgcttcttc catgctaaga cagggtcttc ttcatagaca cgagtcgttt
    57121 tgaaaatctg atcccataat gcctcaactt gtttttcttc tggcagttct gggaagactt
    57181 ttgctgccca ttgtttacca gccgcagcca cgacagtcca actgacttta tttgcttgag
    57241 ttgccttgcg taaattcatt agtgctttgc cattcgctga ttgataagac gctactcgat
    57301 ggctatctac accagcaaat gcatcgggat cggcagaaac aacgctgatc ctgcttgctc
    57361 ctttttcaag ccattcatcc gcttgatcga ttttagattg tggtacattc tcaatacgat
    57421 ccgttgcagc atgtaaaaga aattctcttt ggatctggtc gtcagtccat tgaacgataa
    57481 cctcagcggc tcctagttta taagcttctt gtgtgatcaa tcgagcaagg ggtgcctgtt
    57541 ccacgctgat ctgtaaaaca actgtgtgtc ctttttctgt ggctacgcca gtttctgcaa
    57601 ttaagcgtgc atattttttt agaagttcat taaaatcaga taacataata aaaattcctc
    57661 ctcaccattt tttctggtaa ctattagcat gataccattt aattatgatt caaacaatga
    57721 ggctttaaat aaaaaaagga ggttattttt ttaaggttaa gtttcatgat atatgttact
    57781 acttgctatt tcagcttgtg aagaaaatca aaaactaatt ttgcatatca aaaaaacatg
    57841 ttacaatgaa attaatcaat ggtggaagcg ttataaaaaa attaaggaga ttaaaaaata
    57901 tggcaagaaa aaagacgatt actagagaac agattttggc agctgcttat gaagtagctg
    57961 ccaccgatgg atttacacga tttactgcga gaaacatcgc gaacaaaatg aattgttcca
    58021 cgcaaccgat ttatcttgag tttaaaaata tggatgactt gagacaagct ctttttgagc
    58081 agatctatga tcatttacaa tatgaagtgt tcactgttga gcatacaggg aatacgatta
    58141 tcgatcttgc ttttaattat atccattttg caagaagaga gaagaagttg tatcgttcac
    58201 tgtacttaga agaatatggt ggaggaaaag agatgcagaa tttctcttat acttactttg
    58261 tgaatacagt gaaacgcgat ccagcttatg tgaatctttc agaagagcag atcgaatctt
    58321 tgtataatgg gacatggatc atagctactg ggatcgcagc tttgatgtct tcaagcatca
    58381 tccatccaac tgatgaacag attgaaaaga tgatccaaga cagtatagat gctatcttga
    58441 aattcaaaga accaatcgaa atcgattgaa tatactcaaa agttccacaa atcagtaggt
    58501 aacaagagat gtgtcaaaat aaggaaaagt tcgtttttct tacagcgtat gcatttttaa
    58561 ctcccgaaga actcttccat gaatgcaatg aataaaagag ccatacagat tgaataaatc
    58621 tggatggctc ttttattgca agtatattaa atttttgcga ctagttcttt agcgaaagct
    58681 tctaaatttt cgatatcttc ttcttcagca ttcagatcca ctttaacatt ttcggctcct
    58741 tttgatgcgc ctgtttttgc aaaagcagca tcgaaatcat ccaccgattt gcagaattca
    58801 tcgtaaaaag tatcaccaga tccgcagaca ccgtaaattt ttcccaatag atccaattct
    58861 tgcaagtctt cataaaagtc tacgatctca tcaggcagat cgccatcatc gtatgtataa
    58921 gtagctacta cgcaaatatc tgcctcctcg aattcatctg catctacttg ggtacattcg
    58981 ttgatttcga cttcgatatc cagattttca aatgcttctg caacaatatc ggcgatttct
    59041 tcagtgtttc ctgtcatgct agcatatacg atttttgcta aagccatgat gtgcctcctc
    59101 gtttataatt atttaacaat tatgaattat accaaaacat cattgaaaag aaaataaaac
    59161 ctaaagacac ctggggaatg tcatttggct aaatgtatgc cgctatgtta aaatgagagg
    59221 gatttgtaaa aaggagacga aaatatgatt actttaaaat cagcacgtga aattgaaatg
    59281 atggcagaat ctggagcttt attagcagat gtacataaaa atctgcgtga ttttataaaa
    59341 ccaggaatca caagttggga tattgaggta tttgtcagaa actatatcga gagtcatggc
    59401 ggaattgctg cgcaaattgg ctttgaaggg tatgaatatg cgacttgtat cagtataaat
    59461 gatgaaattt gtcatggatt ccctcgtaaa aagcctttga aaaacggtga tttggtaaaa
    59521 gtcgatatgt gtatcgattt gaaaggcggc atttctgatt cttgttgggc ctatgtagtt
    59581 ggagaatcta cacctgagat cgaccgattg atggaagtaa cgaagaaagc tttgtacatc
    59641 ggtattgagc aagcacaagt agggaatcgg attggtgaca tcggtcatgc tatccaaact
    59701 tatgtagaag gcgaaaatct agcgatcgtt cgtgatttta ttggtcatgg cgttggtcct
    59761 acgattcatg aagaaccagc agttcctcat tacggagaag ctggaaaagg gttgcggttg
    59821 aaagaaggca tggttatcac gatcgaacct atggttaata ccggaacttg gaaaatgaaa
    59881 atggatccta acggctggac tgcgtataca agagacggcg gactttcttg ccaatatgaa
    59941 catacattgg ctattactaa agatggtcca agaattttga catcacaagg agaagaaggc
    60001 acttattgat ctatccagtc aataggtacg ggagaggtac atgaaagttt ggaacaaaat
    60061 aaaacaaaat gaaaatctga tgcgctttat tgagacgact caaaagcgca tcactgattc
    60121 agaaatgagt acgacctctg tcgtcgttgc ttattattta ctcttgtctt tatttccatt
    60181 gattattgct tttggtaata ttttaccgtt tctccacatc gatcaagaga ctgtactgac
    60241 ctatttacga gaagtgattc ctgagaccgt ttaccagttt atcggtcctg caatcaaaga
    60301 ccttttgaca caaagttcag gaggcttgtt atccgtttct gcattagcgg ctttatggtc
    60361 tgcaagtcaa agtatcaatg ctttgcaaat tgcgatgaat aaagcgtatg gcgtagaaaa
    60421 tagaaaaaat tttttgatcg tccgtttctt ctcactaatt gtcattgtct tgttcatgat
    60481 tgctattagt ggggtaacta tcgttcttgg gttgggacag ctgattttgg atgctattca
    60541 gccaatcgta caaatacccg tgaactttat cgatcagttc caaactttga agtggccaat
    60601 cacattggta gctttatttg tcatcatgtt cttgatttat ttaattgtcc ctaatgctca
    60661 gttgaagttg aagacgatca ttccgggcac gatcttcgct actgtcggct ggatgctgtt
    60721 atcacaagtg tttagtatct atgctaggta ttttgcaacg aggatcagcg gttatcagat
    60781 cataggaagt tttattgtac tcatgctttg gcttaatttt gctgctacga tcatcatttt
    60841 gggcggaatc atcaatgcag ttgttcaaga atacgtgacg ggtcatcaaa tcaaaaaacg
    60901 tcgagatcca agccgaaaat ggttcaataa attgaaaact aaattttctc ataaaaaaaa
    60961 gtagaaacaa gagttcttat tgtaaattta tatcagtaat ttataataat tatgactctt
    61021 aattaaagta ggtgcccata tgcttgtttc ttttagtatg gttataagat tttttctgac
    61081 aattttatta tcactgatat taacacctgt attcaaaatc atttcagtac aaacaggtat
    61141 ggtcgacaaa ccaaatgaaa gaagaataaa taaagttcca atgccttcag cgggcggact
    61201 gcctatcttt atttcatttg ttatatcgac tttattctta ttccgtaaaa ttatccctca
    61261 atcgtatatt gtaccgatct tgattgcttc tttagtaatc atagtcacag gagtgctcga
    61321 tgataaatat gagctgacac ctaaacaaaa aagtataggg atcttgattg cttctcttat
    61381 tatttatttt gtagcagata tacgtattga ttccttcacg cttcctttta ttggatatat
    61441 ccaactaggc tggcttagtt ttcccgtaac gattttttgg atcttcggta tcacgaatgc
    61501 ggtcaatctg attgacggtt tggatgggct ggcggcaggg atttcattga tcggtttaat
    61561 tacgatagga atcattggat attttttcct ccatgcttct acagtgtata ttccagttgt
    61621 tattttctgt cttgtagcaa gtattatcgg tttttttcct tacaattttt atccggcaaa
    61681 gatttattta ggcgatacag gagctctatt cctagggttt atgatggccg tcctttcact
    61741 acaagggctg aagaacgtga cttttgtttc atcgatttct ttgctgatcg tcatgggagt
    61801 gccggtaaca gacacgtttt ttgctatcat taggagaaaa gcaaaccgtg tttctttttc
    61861 tacagcagat aaaaaacatc ttcatcaccg actaatcagt ttaggcttta ctcataaagg
    61921 tgctgtcctg acgatttatg cgatggcttt gatgttttct ttcacagcta tggtcatgaa
    61981 ttatactgga agaatcggaa cgatcgtatt gattattgcc atgttgtttg ctgcaatttt
    62041 attatttgaa ttgattggtc tgatcaatga gaaacatcag tttattttaa aaacattgcg
    62101 atttttaggg aataaagagt accgtacgca agtaaaacag aaatatttga agaaatggaa
    62161 ccgtcatata gatgaataac aaattaaacc agccgatcct tattagtatt gtgacggcaa
    62221 acagtgataa aatttttcgg acactagata attttatttc aactgtaaaa gatgaaacag
    62281 cagtaaaaat ccgaattttt gacaacaatt cacttcccga gtacaaaaaa agactaacag
    62341 agtataggaa atatccctac atcgatatct attttcatga tgaaaattcc ggatttggtt
    62401 atggacataa tcacaatctg atagctagcg attctcgcta tggcattatt tgtaacccag
    62461 atattttagt ggatgaagag acagttacag cattggtctt gttattgaaa gaacatccgg
    62521 aatgtgcgat gttagcacct aagattctta atgaagatgg aacaactcag cacctcatcc
    62581 gtgagcagct ggctgttttt gattacgttt tgcgctttgt gcctttcaaa tgggtcaaaa
    62641 aaaagtttga caaacgactg gctgtgtttg aatgccgagg attgcctact gatgctaata
    62701 gttatgtacg gatgatctca ggtagtttta tggtagctga tattgataaa tttaaagaga
    62761 taaaggggtt tgatgagcgt ttcttcatgt actttgaaga caacgacttg tgtttgagtt
    62821 ttgaaaaagc aggttacaag ttattgtaca caccctttta tgcagttatc catttttacg
    62881 ggaagggtgc gcatcgaaat gcaaaactgt ttcgcgtgtt tttgacttct atgatgaaat
    62941 ttttcaataa gtgggggtgg cgttttttct gatgaaacca gaaatattag tagtcgttgt
    63001 gctttatcag caactattca gtcaatctcc agcttatgga ccattaagca aagcacttag
    63061 cgaaaagcaa attcagctta ttatttacga taatagtcct caaaggcagc aaaatgagct
    63121 gtttgaaaaa gaaaatatcg tttatcatca tgatccgcaa aatcctggtt tagcggtggc
    63181 ttataatttt gcgttgtctc aagcagatga aggaacacgt tgtctcgtca ctctggatca
    63241 ggatacgagg ctagtcgacg attattttga actattgcgt aaagtaacct ttacagatga
    63301 atgtgttgct gcagttccga tgattttttc tgggagccga caagtttctc ccctatattc
    63361 agatcgttat atcaatcgta cagctcaagc agtagaagtt gaaggcccaa cagctgaacg
    63421 aatcatggca atcaattcag gtgttgcctg gtctgtcaaa tttctaaaaa agattggcgg
    63481 gtttaatcct gctttttctt tagatttttt ggatcattgg ctgttttgga aagtggatca
    63541 gttggaaaaa actgtggaga tccttcctgt acgaatggaa catgatcttt cggttttaga
    63601 ctatgaaaaa gtttcaagta atcgataccg ttctatttta caagcagaaa atcgatttta
    63661 tcatgaatat gatcaaaggc atttatccag tcaccgacga caattattgt taagaacagc
    63721 taaacagttc ttgaccgtca aaaaccggca tatctggcgg atgactttgc gctcatatgt
    63781 taagaattgg aaggtgtgaa ttcgtgcgtt cggtttgtat agctaccttt aatggaggca
    63841 gatatttaag agagcagtta gatagtattt tatctcagct ttcagcagaa gatgaagtag
    63901 tcatttctga tgatgggtca gtagatgata catgggatat attaactgaa tacgcagaaa
    63961 aagataagcg aatccagctt ttttcggggc ctcgtcaagg attgatcgct aattttggtt
    64021 atgcgatcca gcagacgaaa ggagatctaa tctttttggc agaccaagat gatgtttggt
    64081 tggagacaaa agtgaaaaag atcactgact attttgagca gaatccggat cagctagtag
    64141 tagtttctga tttaatcatt gtggacagcg agctgcaaga gatccatgat tcgtattttt
    64201 cgtttcgaaa ttccaaagaa ggatggctta ataatgtgat aagaagtcac tatattggtg
    64261 ctggtatggc tttccggtct tctttaaaac ccgatatttt gccgatcccg tcagaagtgc
    64321 cgatgcatga tatgtggatt ggtttgctgg gaggaaagaa gactgggttc ctaagagaac
    64381 cgttaactct gtaccgaaga catgatttaa acgcgagtga gattgaaaca cattctaaat
    64441 tacggcaaaa aatttcttgg cgtgttcatc tgattacggc gttgatcaaa cgaaaatggt
    64501 ttaaacgttg ataaaagatg tttaaatcct taatatttcc atgacaaata taacaattga
    64561 tgagataata acaaagtgaa atttgaaatc aaaaaggaga gcagatatcc atgaagggaa
    64621 tcattcttgc tgggggcagc ggaacacgtc tgtacccttt aacaaaggca acatcgaaac
    64681 aattgatgcc gatttatgac aaaccaatga tttattatcc aatgtctact ctgatgttgg
    64741 cgggaatcaa agaaattctg attatttcaa cgccacaaga tacaccacgt ttcaaggaat
    64801 tatttggaaa cggaaatgat ttaggactgc agatcgaata tgcagtacaa gaaagtccag
    64861 atggattggc acaagcgttt atcattggag aagaatttat tggagatgat agtgtctgct
    64921 tggtattagg ggacaatatt tactatggtg gcggtctgtc taaaatgttg caacgagcag
    64981 cttcaaaaga tacaggcgcg acagtatttg gctatcatgt caatgatcca gaacgctttg
    65041 gtgtagtaga atttgatgag aatatgcaag ctttgtcgat tgaagaaaag cctgctcagc
    65101 caaaatcaaa ttacgctgta actggccttt atttttatga caatgaggta atttctatcg
    65161 ctaaaggtat caaaccttca gaacgtggag aactagagat tacggatgtc aataaagcat
    65221 atttggataa aggaaaacta tccgtagaac ttatgggacg cggatttgct tggcttgata
    65281 caggaacaca tgaatcattg cttgaagcat ctacctttat cgaaacaatt gaaaaacgtc
    65341 agaacttaaa agtggcttgt ttggaagaaa ttgcttaccg tatgggctac atcgataagg
    65401 aacaattgct ggcccttgca cagccattga aaaaaaatca atacggtcaa tatttattga
    65461 atttggccgc tgaataagga gtccttacag atgaaagtaa cagatacaaa attaacagat
    65521 gtcaaaatcg tagaaatgga cgtctttggc gatcatcgcg gtttcttcac agaaagttat
    65581 tcaaaagcaa aatttgcaga acatggattg aaatatgact ttgttcaaga caaccattca
    65641 ctatcagctg aagctggtgt ccttcggggg cttcatttcc aaaaaggaga agctgcacag
    65701 acaaaattga ttcgggtagt cactggagca gttttagatg tagtagtgga tatccgcaaa
    65761 ggtagcccga cttatggtca atgggacgga tttatcttat ctgaacataa tcatcgacag
    65821 ttgcttgtac ctaaaggata tgcccatgga ttcgttactt tgacaccaaa tgtgaacttc
    65881 ctttataagt gtgataatta ctataatgca gaagcagatg ggggcatcgc atttgatgat
    65941 ccagatttag cgattgattg gccaattgat ccatcaagag cgattatgtc ggaaaaagat
    66001 caccgtcatc cgacattaaa agaatttgaa gcagaaaatc catttgttta cggagaaatc
    66061 taagaattaa ggagaaattt tcttatgaaa aacatcattg ttaccggagg tgccggattc
    66121 atcggctcta atttcgttca ttacgtggta aacaatcatc cagaagtgca tgtcactgtt
    66181 ttggataaac tgacttatgc aggaaacaaa gagaacttgg ccggacttcc tagcgatcgc
    66241 gtagaattgg tcgtaggaga tgttgcagat gcagaattag tggaccgtct agtaaaagaa
    66301 gcggacgcag ttgttcatta cgcggcagaa tcccataacg ataattcatt aaaagatcct
    66361 ttcccatttg tacaaacgaa tatcattggt acgtatacac ttttagaagc ttgccgcaag
    66421 tatgatgtac gctatcatca cgtatctact gacgaggttt acggcgatct tccattacgt
    66481 gaagacctgc caggacatgg ggaaggcgaa ggagaaaaat tcactgcgga aacaccatat
    66541 aatccttcaa gtccatattc ttctacaaaa gctggttcag atctgttagt aaaagcatgg
    66601 gtgcgttctt ttggcttacg tgcaaccatt tcaaactgtt caaacaacta tggtccttat
    66661 cagcatattg aaaaatttat tcctcgccaa gtaacgagta tcttaagcgg tattcgtcca
    66721 aaattatatg gcgaaggaaa gaatgttcgt gactggatcc atacgaatga tcattcttca
    66781 gcagtgtggc tgattttgac gaaaggcaga attggcgaaa cctatctgat cggtgcagac
    66841 ggtgaagaag ataataagac cgtgatggaa ttgatcttag aaatgatggg ccaaccaaaa
    66901 gacgcatacg accatgtaaa cgaccgcgca gggcacgatt tgcgttatgc aatcgattcg
    66961 acaaaattgc gagaagagct aggttggcag cctgagttta cgaacttccg tgatgggttg
    67021 gctgacacga tcaaatggta cgaagaacat gaagactggt ggaaaaaaga aaaagaggcc
    67081 gttgaagcag catatgccaa aaatggacaa taatacagag gaaatttcac tgtattaatg
    67141 gaaaaacaca tgtgaggcag gcgctcgcct gtctcttttt taaaataaaa atcaaggagc
    67201 ggataaccat atgtttttat taactggagg aaatggacaa ttaggtacag aattgcgcca
    67261 tttattagat gaaaaaaatc tgccatatgt ttcaacagat gcaaaagact tggatatcac
    67321 agatgctgag aaaacaatgg cttatattac agaattaaaa cccgatgtta ttttccattg
    67381 cgcagcttac acagcagtag ataaagcaga agaagaagca aaagaattag atgagaaaat
    67441 caatgtcgat ggcacaagaa atgtagcact tgcagcaaaa gctgtgaatg cacaattcgt
    67501 ctatatcagt acagactatg ttttcgatgg aaaaaacaaa aatgtagaat acgaagtaga
    67561 cgaccaaacg aatccactaa atgaatatgg tcgtacgaaa ttattaggcg aaaaagcagt
    67621 agaagagatt ttagaagact attatatcat acgaacttct tgggttttcg ggatctatgg
    67681 acacaatttc gtcttcacga tgcaacgcct agcggaaacc cgcgacaaac tgactgtagt
    67741 aaatgaccag tttgggcgac caacttggac acgtactttg gcagaattta tggtatatgt
    67801 catcgaagaa aaagcaccat ttggtattta tcatttgtcg aacgaaaata gctgttcttg
    67861 gtatgagttc gcaaaagaaa tattgaaaga tacagatgta gaagttgcac ctgttacgtc
    67921 agaagaattt ccgcaaaaag caacacgtcc gcagtattca gtcatgagcc tgaaaaagac
    67981 agaagcgtta ggttttgtca taccaacttg gcaagaagca cttgcgcaaa tgctggaagc
    68041 tgccaaaaaa atgaataagt aatttccttt ttatcaaata gaatttggag catgatatgg
    68101 aaaaacaaac aagaagtgca cgacgaaaag aaaagccaaa agcgaatcag gcagattcga
    68161 atttggtgct tgacctgttt ctaaaactcc gctatcttat tggcgtttta gtcatcgtgg
    68221 tcatcgttac ttttaattta aacggaagtt cacttggtgt ttgggataaa tatgtgtcgc
    68281 aaagagacga tgggaaaaaa actgatgtga tttttggaca gaatcgggaa gtgcggtcag
    68341 acgaatggct ggtgcagaca cctttttacc tctcacaagc tgaagaggac ttttcgttgg
    68401 tgaacgagga ttattcatta aacggacaaa acatgatcat tgcgtataat tctcctgtaa
    68461 aggatattac ggttatcgga aaaccattca actggggatt ctttttcctc ggaagagatc
    68521 gaggactttc cttttattgg gcgatgaaat tagtcgtcat ggtcttgctt ggattcgaag
    68581 tactgatgat attaacgaag aggaaaaaag ctctatcgct aatcggtgca tttgcgctga
    68641 cattttctcc agcagtccag tggtggttca tgcaacatgt gggcgatttg attttcttta
    68701 ctttgggact gatgattgct ttttatcatt atttctacaa tcatgataaa aaatggctac
    68761 gaacgttgat gatgcttttg gtggttattt tcggtattgg gtttatttta gtgatttacc
    68821 cagcccatca agtgatgctg gcgtacttgc tgatttttta ttttgttggc ctgcttattt
    68881 actatagcaa gaaagtttca tgggatctgt tggatgtagt gttgattgga ggggcagttt
    68941 tatttattgg cggagtcatg gttcatttct ggctaacgtc aaaagacgca ttgttagctt
    69001 ctttgaatac cttatatcca ggaaaacggg taagtactgg aggaaagtgg acgattggcg
    69061 aatttttttc tttccttaca aattggaaaa ttccttttga agatatcaac tattcgaata
    69121 actctgaagt ggccctgttc tatcactttt ttccaactgt attcctagca agtccatttg
    69181 tgttatttgg caaaaaagat agcgaacaaa agttactggg acgtattttg atgatttttt
    69241 gtctgttttc tatcttttgg attaccgttg ggttgccaaa atggctagca gaaatcactc
    69301 tgctgtctta tgttcctcca ttaagagcca tccttacttt tagttttgcg gcttgtttat
    69361 tatcgatctg gtttatcgct tatatttgga gagaagatgt tattcctatt tggtataaat
    69421 tcattttgtt ggcagtaaat gcatggtctt atttctatgc gattacgcat tcgaaaatgg
    69481 ataattattt ttcggatttt gaaaccatcg cagttgctgt gttgggaaca gtgattttag
    69541 cttttgcatt atttggctgg agacgcttgt tttacctcgc ttttgtcgcc ttagttatcg
    69601 tatctggctt tttcgtcaat ccagtcgtag aaggtacagg agcgattttt gaaaaaacac
    69661 tggcaaaaga aatccaaaaa atcgatcaac aagatcctgg taaagtttgg ttgtctgaag
    69721 atgaattata taacttcacc ccaacattgg gagtgaaatc cttcaataca gtacgctttt
    69781 atccagatat agctgcatgg aagaaaatcg atccgaaaga agaatacgag aagtattata
    69841 atcggtatgc tcatacaaga gcttttatcg ctaaggaaga tacaaagttc attttagatc
    69901 gtccagatat gttttcggtc acgttgaatt ttgaagacgc agaaaaatta gggatcgact
    69961 atgtgatcag taaacgacca ttaactgatt acaaccaatt atatgaagcg cagttcacgc
    70021 agctatatgg accggataaa gacggttata tgatttttaa agtagaatat ccggattatc
    70081 cagatgtacc acaaacacaa actcaggaaa atcagctagt cgtacagtaa taggctagct
    70141 gagaataatc ctgaaatatt agtagaaagg agagtgaaga taattcaagc aatcgtacaa
    70201 aaatttgtca acaaaatgag tgtcggtttc ttgcttttgt tgctgacgct agtctttttt
    70261 gtttggacag caggggtagg ttttccagca actttgttta tatataacaa tagttcatta
    70321 cttatcgctt ttagtgtggc tattttgctc attttaaatg tttataaagc aaaaggcgtc
    70381 gattttcttt atctaggtgt tgcgcttgcc cttttcattt tcttttctta ctttcgggaa
    70441 ttaagaaatg agtctttatt tggagactta ctgtttcctt tggttatttt attttgttta
    70501 gtaattaaat ggatcgagtg gacaaaatgg gatcgcggta tttatctgat tgtctttacg
    70561 gtcttcctcg gaatcacgtt atatcgtgta tttacagaga tccaagtacc agaaggacaa
    70621 agcatctggg ctccaggcaa ttcgctttct gatatttgga tcaatacgaa tacaatcgga
    70681 agttctttga tgactcttgc tttattgatc agtggatttg ccagttcttt tgaacgatgg
    70741 tatgttcgaa tcatcggtgg gatagcagtc gttgcagcct ttttaggtat ttgggtctgc
    70801 caatctagag gtgcattagt cgcgttgatt gtttttgcga tattggatct tttacctaaa
    70861 tcgttgatgc gtgtcattcg tgcgccattt attgcctaca cggttacgat tcttttagct
    70921 ttgccgattt cttatctggc ggctgtctct gaaaaagtca atttgtttac cgatcgagaa
    70981 gatatttggc ataaattcta tcagacgcta ggagaaaaat ctgaacaagt tttactagga
    71041 atgaaaacat ttatcttcca gcgaggaaac caatttttag gaaaccacaa tagttataat
    71101 tctattttga atatctatgg attgatagga tttgggattg ctgctttgct gcttatcctt
    71161 tttattggtc gcttaacgtt gaaagcagat ttatccaacg ggcagatgac ttttatctgg
    71221 gccttttttg cggtgatgat gcagtcattt atggaggata cattgacttc atacccatgg
    71281 gttccgatta tttatatcct gttggctatg gccgttcatc gatatgatga accagaagtt
    71341 gcggaggaat tctatgttat ggaagaggca tatgacgata tgacttatga tgatacatct
    71401 tatgaggaac aaccagatag cgctcattct tccaggatcc aacgctatca ttgataggca
    71461 caaaatacga gagaggctgt gacaaccgtc gcagcctttt ttggtttatt gcaattgtct
    71521 agcatcttct gtaaaataga aacgttagag aaaatcttgt atcatagaaa attaagaaag
    71581 aaataagaac gcagctcggt accaaaaata tggtaaaatg gcacttgact gtaattcact
    71641 gaaaatcgct tggaggaaca caaatgttta aagatttgta tttatttatg aaggacattt
    71701 atcagaacag aaaattattg ctacagtttt cgatcaatga cttcaaatca agatatgcag
    71761 gatcgttttt aggaatctta tgggcgttta tcaatccgct atttactgta ttgatttatt
    71821 ggctggtatt tggtgttgga ctgaaagcac aaatgatcga tgcaaaatac ccatttatcg
    71881 tatatttagt taccgggatg attccctggt tcttcttctc agatgcattt gcatcaacta
    71941 cggttgtttt tcgggaatat acttatttag taaagaaagt cgtattcaat attcgtatct
    72001 tgcccacgac gaaaatccta tccaatctgt acacccattt attcttcatt ctgattggca
    72061 tagctgtcgc gcttataaat ggtatatatc cttcagcaat gtctttacag ctgatttatt
    72121 atttattttg catgtgtgct ttcttgacag gattgacttg gctgacggca tcgatccaac
    72181 catttttacc tgatatcatg caattaatta cgatcgtgct gcagttgatc atgtggacat
    72241 taccgatatt atggagccct aatcagtttg attcaggtct tgtaagcata ttgaaattaa
    72301 atccgctgta ctatgttgtt caaggttatc gtgagtcatt tttaagtgaa gcttggttct
    72361 gggagcatgg aacatatact ttgtatttct gggttttcac gttagtaatg ttcgtaatcg
    72421 gctctgcagt gttccgtaga ttgaaaccac atttttcaga tgtgctgtaa ggagaatagc
    72481 tatgacagaa tatgcaatta gactaaaaga tatcacaaaa tcttataata tgtacgcaaa
    72541 accttcggat cgatttcgtg aagccctcaa tccttttaag aaatcctatc atgatgtatt
    72601 ttatgcgttg aaaaacgtga atgtagaaat aaaaaaaggt gagatgatcg gtttcgtggg
    72661 agaaaacgga tcgggaaaat ctacaatgtt gaaaatcatt acgggagtac tgacaccaac
    72721 atccggcacg atggaaatcg aaggaaaaat ctcagctttg ctagaactag gatcaggatt
    72781 caatcctgag tattctggct atgaaaatat ctatttaaat ggaatggttc taggattttc
    72841 aagagaagaa gtcgatgaaa tggtcgatga tatcattgaa tttgctgaca tcggagacca
    72901 tatttatcaa cctgtaaaaa cttattcaag cgggatgttc gttcgtctag cttttgctgt
    72961 agcaatcaat gtgaatcccg atattttgat tgtggatgaa gcattggcag taggcgatct
    73021 tgaatttcaa ctaaaatgta tggaaaaatt tacagaaatc aagaactcgg gaaagacgat
    73081 tttattcgtt tctcatgata tcagttcgat tcgtcgtttt tgtgatcgtt cctattggtt
    73141 gaaaaacgga gaagtcgtcg aatatggcga tacgatggaa gtaacgacga attatgaaaa
    73201 ctttttaaag aaaaaatcag ttaaaacagt ggatcggaat aaaaaccaag tagaacggtt
    73261 cgccgctcca gatatcgtcg acgtgacgaa agcggaattg ttggataaag acaaaaatcc
    73321 ggtggatatg ctggaacaag gagaaaaact atacgttaaa gtaacttatg aagtaaaaga
    73381 tgatacaatc aaaaatccgg ttttaggaat tgcgctacga acagtcgaca atttttatgt
    73441 atgcggactt aatactctac tagatggtat caagattcct tggaaaaaag gagagaatat
    73501 cttttatctg gagtatgaaa agttaggttt gttagctggt gaatattatt ttgacgttgc
    73561 ggtctttgaa gaaaatgcaa cggttccttt ggtatataag acaaagtaca tgaacttgtt
    73621 tgtcagcggt tcttatatcg gagaaggcat cgtggtctta gaccataaat gggaagaagg
    73681 gactcatagc aatgaaatat gattttgaaa tggacctaga tgaacaaagc agtgtgggaa
    73741 aaattgccgc acagatcaaa ccaggaagta aagttttaga gtttggtccc ggaaacggcc
    73801 gattaacgaa acatttaatc ggagcaaaac aatgtgaagt gagcattgtt gagttagata
    73861 aggaattgtt cgattttgtc agcgaatttg ctcaagatgg attttatggt gatatcgaaa
    73921 gttttgagtg ggcaaactat tatgctggac agacatttga ttacgtcttg tttgcggatg
    73981 ttttagaaca tttggtagat cctggaaaaa cattgaagaa agtcagagaa tttttgaatg
    74041 aagaaggaga gatcctgatt acattcccta acttggcgca taattctgtc atgatcgatt
    74101 tgttcaataa ccaattgccg tgggcttctt atggtctcct agatgaaacc cataattctt
    74161 tttatactca tgatggcttc caaaaagttt ttgaaaaagc gggattattc attaatattg
    74221 aagattattt gtacttagct gtaggtgata cagaattaaa atcaacttat gaagaattgc
    74281 cggaagctgt acgctatgat ttcaaaatgc gaccatttgg tgaagtctac caatactttt
    74341 tctcattgat gaaacatcca gtagcacaaa gttccattgc agagccacaa aattctaatt
    74401 atgtaaaagt gttagaagta acccagcaaa cgaaacaaga tgaaacaatc caacaaatac
    74461 cattcaataa tttcacaggg gaaaatgaga cactaagttt ccccgtatca gaaacaacag
    74521 aaagaatggt gtttcgcttc gcgcaacaac ctagctttat cgaattcagc gcagaagctg
    74581 ccggagaaaa actaacattt atcgattcga atgctgtagt caaaacagta aatgattgtt
    74641 atttgtttga tgggaaagaa ttaccagagt tcgttctaac ggatatttct gggaaagaag
    74701 tgactatcca ttgtcactat cgctttatcg gggaattgac accaacaatg aaagaattgt
    74761 tggaaacgat tcgtcccatg gctgaagtca caaagcagct ttctgagaaa aatgacgaat
    74821 tagaaagaga aaatcatcat cttcttgagg aaaatacaag actagaccat acgttaaaaa
    74881 caaccacaaa tcgctattgc acattgttgg aaagtgatga atggacaata aaacatagac
    74941 taggacgacg taagaaagaa acttcgaaaa aaatccaaga aaaagaactt tctatatgta
    75001 ttgacgagaa aatctgggat gcagaaacaa aaatattaaa aatcatcgga tggggaatcg
    75061 ctaattcgga tcgccagccc ttgtcctata agttatcagc tgaccaatcg ccatttttcg
    75121 aagcaattcc tgtttcacga gaagaagtca atcaagcaga acagctcgca aaaggaacag
    75181 aagcgggatt tgagttacga atcctttgcg agcaagaacg ttcattctta gtggaagcag
    75241 tagctcaaaa tggccaatca tggatcattg aaatgtaaat aatgattaac ttataaagat
    75301 aaggagtaaa cgaatcgtgc aaaaaaaaga aattgccgta tttatcgatc aaatcactcg
    75361 tgaacgggcc actggcgatt tgcttattgt gggctgggca attgatgaag tgaccaaaga
    75421 aatccctacg ataaaggtag aaaaagaaaa tgtgattgct gaagcaacac atgtcgtaag
    75481 attggatatc aatcatttat ataatcttga tgttaagacc caaagcggtt tcaaggtacg
    75541 tttatcaggg aaaatgagag gaaaagccat cttagacttc caaacagcga gacatcaaaa
    75601 tggcatagct gtgaaattaa acggtaaata cccttatgat gacggcattg aatcttcatg
    75661 ggaaagaaaa aaaagattac tgaaaaaagg gataaattat gcaaggactc atggcgtaaa
    75721 aaaagcgatc cgtcgggtca aattggaatt gaagccagga tcgatcgact atgcagagtg
    75781 gatcagtcgt catgagaaac cagatttgaa agagcaaagg aaagaagcac aaggatttgc
    75841 gtaccgtcca ttgatttcca tacttatgcc tgtctataac gttgaaatca aatggctcga
    75901 aaaatgcatt gattcggtac tggatcaaac gtatgatcat tgggaactat gtatcagtga
    75961 tgatgcttct actgatccag ctatccgaaa gtgtctggaa tcctatcaag caaaagatga
    76021 tagaatcaaa gtcgttttcc gaaaagaaaa tggacacatc agtctggcaa ctaattctgc
    76081 attggaaata gcagaaggag aatttatagc actcttagat aatgatgatg aattaccgcc
    76141 atttgcatta tatgaagtgg caaaagtgct aaacgtacat ccagaactgg atgtgatcta
    76201 cagtgacgaa gataaaatcg atgcagatgg caatcgcttt gatccgcatt tcaaagcaga
    76261 ctggtcgcct gatacattga tgggaaataa ttatatctct cacttaggcg tatatcgaac
    76321 aagcattgtt aaagaacttg gcggtttccg taaaggctat gaaggttctc aagattatga
    76381 tttggtcctt cgtgtgacag aacaaatccc tgcagatcat atttaccaca ttgaccgagt
    76441 cttgtatcat tggcggacga tcccaggatc aactgcaagt aatggcgaag ccaaaagtta
    76501 tatctatgat tccggagtga aagcattgac agatgccctg tctagaagaa acatcaaagg
    76561 aagcgtacgt cctggcagaa tttctggatt ttatgagatt gcttatgacg tcttgcaaga
    76621 ggatttggtc agtgtgatca ttccaacgaa gaatggctat gaagatctaa aaacttgtgt
    76681 ggattcgatc atcgaaaaaa cgtcttattc aaattatgaa atcattattg cggataacgg
    76741 aagtacagat ccaaaaatgc aagaattatt tgcagaatac aagcatcagc tgaaagaccg
    76801 ctttatcgta gaattgattg acattccatt caattattct cgaattaaca atttagcagc
    76861 agaaaaagca aacggcaaat actttttatt cttgaataac gatacagaag tgattgagcc
    76921 agattggatg acggtgatgg tttcttatgc acagtttgat cgaatcggct gtgtaggagc
    76981 aaaactgttt tatccagatg atacaacgca gcatgcagga gtattattag gaatcggtgg
    77041 tgttgcaggg catgcattga ataactatga tcgaacacat tgtggttatt tcggccgatt
    77101 ggtcattgac gtcaattact tagcagtcac tgcagcttgt atgatggtca aggcagctga
    77161 cttcaatgct gttaacgggt ttgatgaaac attagaagta gcattcaacg atgttgactt
    77221 gtgcttgaaa gtttatgaac tgggtcgtta caatgtctat gcacatcaag cagaattgta
    77281 ccatttcgaa tctaaatcaa gaggttatga agatactcca gaaaaacaaa gacgttttgc
    77341 tggtgaaatc aaaaaaatgc aagataaatg gccagcatat attgcgcatg atccatttta
    77401 caatgataat ttaacaaaag acggaatcgg cgacttttcg ttgagaccag attaaactat
    77461 aataaaaagc tgttatgttg tgattcgctc gcacataacg gctttttata gttttttgtc
    77521 aaaaaaaggc agaaggtgcc gactcaaaaa aataagtcga ggttgggaca gaagtgttta
    77581 actccgagaa ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcggc
    77641 ttatttaaga agaagttgct tctgctccca ccgtttattc gtttttaaag aatgagacaa
    77701 aagtgtttct tacttttgtc ccacgctctt attcatgctc agagcgccaa tagatacaaa
    77761 atcatagcca aagtttcacg aaccctagct gtcaaagaat cgatatttta ttttaatata
    77821 ggctatttta aaataaatgg tttaaggtat tcctctacaa aagccactct atcaattatc
    77881 atgggtttaa aataagaagt gatggagata accagattgt tcttagcgtc aatataaaga
    77941 acatttccac tattaccaat tgctgaatat atttcgttct cagaatctaa tacccaccat
    78001 aaatagccat atctcatatt ttgtaagtct tctaaagcac tggttctagg tttgaacatc
    78061 tcgtttatcc acatgggtgg tacgatttgt tttcctttat acatcccttt atttaggcat
    78121 aatacaccaa tttttgccag atcacttgcc gataaacaca aaccatatcc tgccgtcccg
    78181 atatttagtg gatcacaaaa ccagacattg gttttaggag acttatccat tgtaaactgc
    78241 ttatgttctt cagctgtttc agcataataa ttgacatggt ttggtatccc tataggatca
    78301 aatagatact tattggcaaa atcaacagtt gttagcttac ttgtctgata caacaagcca
    78361 gttaatatgt gtaaacagac agtttgatag ttgaattcgc cggttatacc ttttcttcct
    78421 cccaataaat ctagtgatgt ttttgtccaa tcttcacttg aacagacttt tgtccatgga
    78481 tcgcctttac atttgtaggg tgctttcatt gttagcaagt gcttgattgt aatagcgtaa
    78541 atagttttct ccccgcgttt cactttatac tctggaaaat aatctaatac tttatcatcg
    78601 atacttttga ttaatccttg ctctaaagca atgccaatca gcaaagatac aatacttttc
    78661 gttgctgaca tgatatgaca agtatcgtct tttttataac cattccattc atctgtataa
    78721 acttctaccc catccttata tgcgacaatt tgacatatat ttggttgtcg aaaaccaata
    78781 aaatcatgca actcttgttt attcataaga tattacctca ctttcttcta taagcataat
    78841 gtcattaaaa atcgatttca attttttcga ttcttcacgt aatacaggta ttcttatgga
    78901 ctagacagcg ctttgggaag atggattctc tgaaaaatat aaaggttcac tttatgagtg
    78961 gataaaaaaa gcagcaaata cttcgacttc tttaatttac caacattagt gaaaatcgat
    79021 tcatctggag atttgtaatt tagagtcctt gtcttgatta gaggtgtata aagcttttta
    79081 ttagacattt ttaaattcca ttacaatttg cttataaact aggtttttaa aggtgactat
    79141 ggtaagataa tagagatatt attttgtgat taggagtgta tatatgaata aaaatggaga
    79201 atggagtgct gcacgacgga tattcattat attaatggat gttattgtat acaatgcagc
    79261 aatatatagt agtttcttac tgaagtttcg tggagagatc ccattacgaa atttagaaac
    79321 tttccaacac tcagcaatta ttatttcagt gatttttatt gcgttgaaca ttttgttggg
    79381 tgcatatgtt ttttataatc gaatgatcag tgacatcgtg tttgtcacag tcatcggtca
    79441 ggtattgatg acactaggga tcatggtagt gacttttgcg ggccgctggt ttgcttttcc
    79501 acgtcttgtt atcttacttt catttgttct cggtacgctt ttcttaagta tctatcgtat
    79561 tctcgtatat aaactttatt tacgcgtaag tcgagataaa aaggtcgtca ttattgggtt
    79621 agaaaaggat gtcgcaccag caatccaaaa ttttggttca aagaaaaaca acaaacataa
    79681 agtcgaagca gtcattcttg ataactatta tgcaaacatc aaaaatatga ttaaagacat
    79741 cgatattgtc tatcttgctt ctcatatcga agaaactgaa aaaatcaaaa tctatcaatt
    79801 agttactaaa aatgaaaaaa aattgttttt gaatacaaca tttgaaaact tgacattgat
    79861 caatccaaat attatgaatt tcgaagatga aagtattatt gaagcatctg gatttagaat
    79921 ccaacctgaa tatgaactat tcaaacgttt gtttgatatc atcgtttcac ttgttttatt
    79981 agttattgct tcaccattta tgttattcac agcaatttta gtcaaagtga catcaccggg
    80041 accaatcatc tataaacaag tgcggatcac aaaaaatcaa aaggaatttt caatctataa
    80101 attccgctcg atgaccgcca cagccgaagc aaaatctgga ccagttttag ctaagagcaa
    80161 tgatgcacga gtaacgcctg taggtaaatt tattcgagct gtccgctttg atgaattacc
    80221 acaaatcttc aatgtgctga aaggcgatat gtcgatcgta ggaccacgtc ctgaacgttc
    80281 tttcttcgtc gatcagttca atgcagaaaa cccttattac tacttacggc ataatgttcg
    80341 tgctggtatc actgggtatg cacaagtcta tggaaaatat gtctctgatt acaacagtaa
    80401 attacgtttt gatttacttt atatcaaaaa atattcattg atgcttgatt taaagatctt
    80461 gctgcaaacc atcaagatcc tatttgataa aatgtcctct caaggacttg aagaagacga
    80521 aaatgtgatc tcattggcag aaattgcttt tccgaatgaa aaaatctttc gctagttggg
    80581 aggcgtaaaa atgaacaatc cgaaattcag cataatcgta ccagtataca atgcagaagc
    80641 aactattggt cgaacgattg atattctaaa agaattggat tatgaagagt acgaagtcgt
    80701 tttagtaaat gatggatcaa cagatcaaac acaggaaatc attcaagaac tgattgaaaa
    80761 tgatgaacgt tttgaattga ttactacgac caatcaaggg cccggattag caagaaacga
    80821 aggaataaga cagtcaaaag gagaatatct cttattcttc gatgcagatg acacgccagt
    80881 aaaaacgatc ttgtcagatt atgatcattt gctagaaaag agtccaaaag cggatcttgt
    80941 tgttagttcg tttgtttttc gtacaatgga cgagggaaga atcgtttccg aaaaagaaaa
    81001 cttagttaca gaatgtcact atctaactca cgaagagttt ataaaagaca tgtatcagtt
    81061 aatgaatgaa cagttgatgt atgttgtatg gaacaaatgc tatcgtagcg atattctgaa
    81121 aaataaccaa attctattta aaacttataa tagttgtgag gatcgaatat ttaacttaca
    81181 ttattatgaa tactgtcatc aagtgatcat gaacccaaaa gtagaatata tttatgaatt
    81241 tgaaggtggg aaggggatca ctaatcaata ccgacctaac aagttcgaaa catttaaaga
    81301 attctattca ttagcaaata ctgtaacaga cgatattaat aaagacggta tggcggcgct
    81361 tcttttaaaa gggacaactt cagttatttt ttctatttat agtacctctg caagaacttc
    81421 aaaagagaag aaagacgaag caaagcagat attgagtgat ccagtgatcc gagaagccaa
    81481 gcaaattgct aagacggatt cttcagcaaa aaaaataaca aagttgcttt acaatatgcc
    81541 agctccacta tttttatctg ctgtaaaagt tggaagcttc gttgaagtga aaatgccagg
    81601 gttaatggct gctttgaagc gagtatatta gttaatggag gaaaatataa tgaaaagaat
    81661 tttgcatttt cagggacgaa tgggtttagg tggagcagag tctttcatga tgaatttata
    81721 tcgtaaaatt gatcgtacaa actatcaatt tgattttttg atttatgaag attatgcaga
    81781 tgttcaagac tatcattcag agattgaaag attaggaggc cgtatttttg ttgtacctaa
    81841 tcctaagaaa aatattttta aatatttaat agaagtgaat aagttactaa aaaaagaatc
    81901 tttttctata gttcacaatc aggtttattt tggcggggga attaatttat ggttagccaa
    81961 aaagaatgga atcagacaga gaattgctca tagccatgca actgaagatg gaaaatctca
    82021 aaatattgta atgaatgtat tgcgtaaatt tttaaccaaa ttgttgttac aaaatgcaac
    82081 agactacctt gccgttagtc aagaagctgg tgaaagtctg tttcaaaacc atccctttga
    82141 gatagttcat aatggtattg atttagagct ttatagtaaa aattcagaag ctaaggttaa
    82201 taagagaaaa gaattggata tctcaatgag tacttttgtt gtgggaaaca ttggacgatt
    82261 agagaaacaa aaaaatcaat cttatttgtt agagattttt ttagaaatac taaaaatgaa
    82321 ttctgattct tacttgattt tagtaggtga agggtcactt agaaataaac tgaaagccaa
    82381 agcagtagaa ttggatatag agaaaagagt aaaattttta ggagaaagaa acgatattcc
    82441 cgagttatta tctgtgatgg atgtttttgt aatgccttct ctttatgaag gtctgccaat
    82501 ggttggatta gaagcacaag cagcaaaatt aaaattagtc ttagcagata ctatttccaa
    82561 agatacaaag ttaacaaata atgtggattt tgtatcatta gatgaaacac cacaagaatg
    82621 ggcaaaaaaa gtaataaaag agccgtatgc taataaaata acacaagaac taaagagtta
    82681 tgatgtttcg tacactactg aacaaatgga gagtatatat agtagagatc attaaaaatg
    82741 ttcaagatat tttaggagtg attatttttg aaaatatgtt atttgatatt ggcatacaat
    82801 gatattggca acttaaagag attggtcaat cgattgaacg aaaatgcagt ttttattatt
    82861 ca
//
LOCUS       DARBFO010000005        76787 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000005 DARBFO010000000
VERSION     DARBFO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.005"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(146..670)
                     /locus_tag="SCK51_000443"
     CDS             complement(146..670)
                     /locus_tag="SCK51_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1251094.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(663..1136)
                     /gene="tsaE"
                     /locus_tag="SCK51_000444"
     CDS             complement(663..1136)
                     /gene="tsaE"
                     /locus_tag="SCK51_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEG1251095.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1259..2239)
                     /gene="pta"
                     /locus_tag="SCK51_000445"
     CDS             complement(1259..2239)
                     /gene="pta"
                     /locus_tag="SCK51_000445"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEG1251096.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2392..3072)
                     /locus_tag="SCK51_000446"
     CDS             complement(2392..3072)
                     /locus_tag="SCK51_000446"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEG1251097.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3198..4067
                     /locus_tag="SCK51_000447"
     CDS             3198..4067
                     /locus_tag="SCK51_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1251098.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4093..4608
                     /locus_tag="SCK51_000448"
     CDS             4093..4608
                     /locus_tag="SCK51_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251099.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4655..5248)
                     /locus_tag="SCK51_000449"
     CDS             complement(4655..5248)
                     /locus_tag="SCK51_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1251100.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5273..5452)
                     /locus_tag="SCK51_000450"
     CDS             complement(5273..5452)
                     /locus_tag="SCK51_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251101.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5529..6038)
                     /locus_tag="SCK51_000451"
     CDS             complement(5529..6038)
                     /locus_tag="SCK51_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251102.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6134..6514)
                     /locus_tag="SCK51_000452"
     CDS             complement(6134..6514)
                     /locus_tag="SCK51_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1251103.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6844..7593
                     /locus_tag="SCK51_000453"
     CDS             6844..7593
                     /locus_tag="SCK51_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEG1251104.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7777..9054)
                     /locus_tag="SCK51_000454"
     CDS             complement(7777..9054)
                     /locus_tag="SCK51_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251105.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9133..9777)
                     /gene="pcp"
                     /locus_tag="SCK51_000455"
     CDS             complement(9133..9777)
                     /gene="pcp"
                     /locus_tag="SCK51_000455"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEG1251106.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9976..10920)
                     /locus_tag="SCK51_000456"
     CDS             complement(9976..10920)
                     /locus_tag="SCK51_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEG1251107.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10922..11608)
                     /locus_tag="SCK51_000457"
     CDS             complement(10922..11608)
                     /locus_tag="SCK51_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEG1251108.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12082..12402
                     /locus_tag="SCK51_000458"
     CDS             12082..12402
                     /locus_tag="SCK51_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEG1251109.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12556..13461
                     /locus_tag="SCK51_000459"
     CDS             12556..13461
                     /locus_tag="SCK51_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1251110.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13458..14231
                     /locus_tag="SCK51_000460"
     CDS             13458..14231
                     /locus_tag="SCK51_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEG1251111.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14412..15836)
                     /locus_tag="SCK51_000461"
     CDS             complement(14412..15836)
                     /locus_tag="SCK51_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1251112.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16047..16493
                     /gene="nrdI"
                     /locus_tag="SCK51_000462"
     CDS             16047..16493
                     /gene="nrdI"
                     /locus_tag="SCK51_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEG1251113.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16558..18540)
                     /locus_tag="SCK51_000463"
     CDS             complement(16558..18540)
                     /locus_tag="SCK51_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1251114.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18554..19450)
                     /gene="murQ"
                     /locus_tag="SCK51_000464"
     CDS             complement(18554..19450)
                     /gene="murQ"
                     /locus_tag="SCK51_000464"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEG1251115.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19453..20514)
                     /locus_tag="SCK51_000465"
     CDS             complement(19453..20514)
                     /locus_tag="SCK51_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEG1251116.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20725..21291)
                     /locus_tag="SCK51_000466"
     CDS             complement(20725..21291)
                     /locus_tag="SCK51_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1251117.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(21319..22821)
                     /gene="eat(A)"
                     /locus_tag="SCK51_000467"
     CDS             complement(21319..22821)
                     /gene="eat(A)"
                     /locus_tag="SCK51_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEG1251118.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23079..24200)
                     /locus_tag="SCK51_000468"
     CDS             complement(23079..24200)
                     /locus_tag="SCK51_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG1251119.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24326..25267
                     /locus_tag="SCK51_000469"
     CDS             24326..25267
                     /locus_tag="SCK51_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG1251120.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25417..26313)
                     /locus_tag="SCK51_000470"
     CDS             complement(25417..26313)
                     /locus_tag="SCK51_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEG1251121.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26582..27922
                     /locus_tag="SCK51_000471"
     CDS             26582..27922
                     /locus_tag="SCK51_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1251122.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28140..28583
                     /locus_tag="SCK51_000472"
     CDS             28140..28583
                     /locus_tag="SCK51_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEG1251123.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29087..30505)
                     /gene="nhaC"
                     /locus_tag="SCK51_000473"
     CDS             complement(29087..30505)
                     /gene="nhaC"
                     /locus_tag="SCK51_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEG1251124.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30908..32131)
                     /locus_tag="SCK51_000474"
     CDS             complement(30908..32131)
                     /locus_tag="SCK51_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEG1251125.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32162..33001)
                     /locus_tag="SCK51_000475"
     CDS             complement(32162..33001)
                     /locus_tag="SCK51_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEG1251126.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(33018..33698)
                     /locus_tag="SCK51_000476"
     CDS             complement(33018..33698)
                     /locus_tag="SCK51_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1251127.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33700..34773)
                     /locus_tag="SCK51_000477"
     CDS             complement(33700..34773)
                     /locus_tag="SCK51_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1251128.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(34829..35067)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(35110..35343)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(35420..36091)
                     /locus_tag="SCK51_000478"
     CDS             complement(35420..36091)
                     /locus_tag="SCK51_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1251129.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(36178..38856)
                     /gene="mgtA"
                     /locus_tag="SCK51_000479"
     CDS             complement(36178..38856)
                     /gene="mgtA"
                     /locus_tag="SCK51_000479"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEG1251130.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            39515..39952
                     /gene="ndk"
                     /locus_tag="SCK51_000480"
     CDS             39515..39952
                     /gene="ndk"
                     /locus_tag="SCK51_000480"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEG1251131.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39974..40840)
                     /locus_tag="SCK51_000481"
     CDS             complement(39974..40840)
                     /locus_tag="SCK51_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1251132.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(41000..43093)
                     /locus_tag="SCK51_000482"
     CDS             complement(41000..43093)
                     /locus_tag="SCK51_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG1251133.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(43842..44465)
                     /locus_tag="SCK51_000483"
     CDS             complement(43842..44465)
                     /locus_tag="SCK51_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251134.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(44624..46021)
                     /locus_tag="SCK51_000484"
     CDS             complement(44624..46021)
                     /locus_tag="SCK51_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1251135.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(46053..47024)
                     /locus_tag="SCK51_000485"
     CDS             complement(46053..47024)
                     /locus_tag="SCK51_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1251136.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            47398..47823
                     /locus_tag="SCK51_000486"
     CDS             47398..47823
                     /locus_tag="SCK51_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251137.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(48311..49012)
                     /locus_tag="SCK51_000487"
     CDS             complement(48311..49012)
                     /locus_tag="SCK51_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG1251138.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(49028..49468)
                     /locus_tag="SCK51_000488"
     CDS             complement(49028..49468)
                     /locus_tag="SCK51_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEG1251139.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(49468..50043)
                     /locus_tag="SCK51_000489"
     CDS             complement(49468..50043)
                     /locus_tag="SCK51_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEG1251140.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(50891..51370)
                     /locus_tag="SCK51_000490"
                     /pseudo
     CDS             complement(50891..51370)
                     /locus_tag="SCK51_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(51529..52230)
                     /locus_tag="SCK51_000491"
     CDS             complement(51529..52230)
                     /locus_tag="SCK51_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1251141.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(52338..53747)
                     /locus_tag="SCK51_000492"
     CDS             complement(52338..53747)
                     /locus_tag="SCK51_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEG1251142.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(53795..55660)
                     /locus_tag="SCK51_000493"
     CDS             complement(53795..55660)
                     /locus_tag="SCK51_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1251143.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(55781..56632)
                     /locus_tag="SCK51_000494"
     CDS             complement(55781..56632)
                     /locus_tag="SCK51_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1251144.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(56942..57655)
                     /locus_tag="SCK51_000495"
     CDS             complement(56942..57655)
                     /locus_tag="SCK51_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEG1251145.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(57723..58049)
                     /locus_tag="SCK51_000496"
     CDS             complement(57723..58049)
                     /locus_tag="SCK51_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEG1251146.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(58172..58399)
                     /locus_tag="SCK51_000497"
     CDS             complement(58172..58399)
                     /locus_tag="SCK51_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEG1251147.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(58721..59974)
                     /locus_tag="SCK51_000498"
     CDS             complement(58721..59974)
                     /locus_tag="SCK51_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG1251148.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            60035..60775
                     /locus_tag="SCK51_000499"
     CDS             60035..60775
                     /locus_tag="SCK51_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1251149.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(60861..61619)
                     /locus_tag="SCK51_000500"
     CDS             complement(60861..61619)
                     /locus_tag="SCK51_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG1251150.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(61616..62644)
                     /locus_tag="SCK51_000501"
                     /pseudo
     CDS             complement(61616..62644)
                     /locus_tag="SCK51_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(62664..65831)
                     /locus_tag="SCK51_000502"
     CDS             complement(62664..65831)
                     /locus_tag="SCK51_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251151.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(66055..66939)
                     /locus_tag="SCK51_000503"
     CDS             complement(66055..66939)
                     /locus_tag="SCK51_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEG1251152.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(67034..67471)
                     /locus_tag="SCK51_000504"
     CDS             complement(67034..67471)
                     /locus_tag="SCK51_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251153.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(67688..68599)
                     /locus_tag="SCK51_000505"
     CDS             complement(67688..68599)
                     /locus_tag="SCK51_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEG1251154.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(69056..69484)
                     /locus_tag="SCK51_000506"
     CDS             complement(69056..69484)
                     /locus_tag="SCK51_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251155.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(69512..70150)
                     /locus_tag="SCK51_000507"
     CDS             complement(69512..70150)
                     /locus_tag="SCK51_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1251156.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(70154..72136)
                     /locus_tag="SCK51_000508"
     CDS             complement(70154..72136)
                     /locus_tag="SCK51_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEG1251157.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            72316..72981
                     /locus_tag="SCK51_000509"
     CDS             72316..72981
                     /locus_tag="SCK51_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1251158.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(74916..>76787)
                     /locus_tag="SCK51_000510"
     CDS             complement(74916..>76787)
                     /locus_tag="SCK51_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG1251159.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT        22275 a        15952 c        13079 g        25481 t
ORIGIN      
        1 tttttgagct aataccgaag gtcagctgat tgaacaccgt ttattcaaaa aaaatggact
       61 gcgacacgac ttttgtcaca gtcccttcgt gtgtttttct gccttctcaa aaggatgaca
      121 gtttctcact cttttcctat ttcttctatt caatcaaata actcatttta tatacatcta
      181 aaaattcacc tagatctgtt cttgcccctc taggcatgac cgcttcgatt tggaagccca
      241 tttttcggta aaggtgtacg gctctttcat tacgaacttg aacgtcaagc tccaatcgaa
      301 aaagtacacc catttccttc gcccaactga tgatttcttc tagcataaga gtgcctagtc
      361 ccattcccca atattcctgc agaatactga taccgacttc tcccacgtga gacaaacgaa
      421 attgactgtc tgccttgaca gatgctgttc caataatcgt cccttcatag attgcaagca
      481 gcaataaatt attattgctt tcgtatatat aatccaactc ttcttctaag gtttctggtg
      541 gtaacaggag ttctgcttca tccaaaacta aaaattctgt ctccttatta acttgtccca
      601 tcatcaaaag aatgtctttt gcatctcctg gcactgcttc ccgcaagatg atcccaactt
      661 cttcactcat gattttcagc ccatttttct agaattcgct gtttgaaggt ttcactttgc
      721 tccccgtatg agattagttt cacataccgc ttttctaaat ctgtatcact tttttctaaa
      781 atcagttcca aataatcctc cggcagtgct tcttctaata gatttcccca ttcaacaacg
      841 gaaagcccat caccttcaaa atattcatct aaccctaaat ctgctccgct agctgctacc
      901 cgataaatat ccatatgata caacggaatc cgaccttgac tatattctct aataatcgtg
      961 tatgtcggac ttttgatcat ttgcgttatc cctaatcctt gggcaattcc tttcgtcaac
     1021 gttgttttac ccgcacctag atcaccagtc aatatcagat tgtcacctgg cattgcggaa
     1081 tgaccaattg cctctgcgaa ttgttctgtc attgttaatc ctgatagttc gatcattttt
     1141 catcttctcc cgctcttctt tctttttgcc agtatatcgc gtaatgaagt ttttttaaag
     1201 aaaaaatgaa ctagtcaaga acagcagctg ctgtttttgg ctagttcgcg tgtatttgtt
     1261 aatcaatcaa tgtttgtgct gctgtgataa tcgataattt atatacatct tcttcatttg
     1321 ctccacgaga caaatcagaa accggtttgt tcaagccttg tagaattggt ccgattgctt
     1381 caaagttacc gaaacgctga gcaattttat agccgatatt tcctgactgc aattctggaa
     1441 atacaaagac tgttgcttga ccagctactt ctgaatctgg cgctttcagt tgtgcaactg
     1501 aaggaacata agaagcatca aattgcaatt cgccgtcgat catgtattgt ggcgctagtt
     1561 ttttagcgat tttagttgct tcaactactt tgtctacttc tggcgatttc gctgagcctt
     1621 ttgtcgagaa gctcatcatt gctacttttg gttcgatatc gaacaattct gctgtttttg
     1681 cagaatctac ggcgatttca gccaattctt gcgcagttgg attcacgttg atcgcacaat
     1741 ccgaaaagac atatttttct tgatctcttc cacgtaccat gatcatcgct ccgcttgtcc
     1801 gactgatgcc aggctttgtt ttgatgatct gaagtgctgg gcgaacagtg tcgccagtcg
     1861 aatggatcgc tccagagacc attccgtctg caatccccat atacgtaagc atcgtaccaa
     1921 agtaattcac atcttttaaa atcttttctg cttgttcttt ggatgccttt ccatttctac
     1981 gttcaacaaa agtatccacc atttcatccc acttatcgta attatcaggg ctgatgatcg
     2041 taaagctaga tgttttgatt cctctggcat gcgctgcatt tcttatttct tcttcgttcc
     2101 ctactaaaat gggttccaca agttcttctg ctttcaaccg ggcagctgcg cctaaaatac
     2161 gagcatcagt agcttccggg aaaacgattt tgatcccacg gcgaacaatt ttaaatctta
     2221 aactatcaaa caattccaca agatgacctc cagacgtttt tatatgtatt atcatacact
     2281 attccagaaa aaaagaacag tttattttat ctgtatcagt caaaaatgct ggaaaaatta
     2341 agtttttcac atacaaagcg aactgcacta taacaggaaa gcgctgtttt attacactgt
     2401 ttcaggcagt tgccaatcaa tcggttcttc tcctaacgca aggagcgcat cattcgtttt
     2461 cgagaagggt ttggaaccaa aaaagccacg acttgcagat aacggactgg gatgtggaga
     2521 tgttatgatg atatggcgac tcttatcgat cattttgatt ttttcttgtg ccggttttcc
     2581 ccacaagata aacacgattg gtttttcccg ttcatttaat ttttcaatga tcgtgtctgt
     2641 taatctttcc caacctttcc cgcgatgaga atacgcttgc ccttcccgaa cggtaagaac
     2701 ggtgttgagt aaaaggactc cctgtttagc ccatgacact agatttccat gttgaacagg
     2761 tgcgattcca agatcatcat aaagctcttt atagatattt cttaacgatg gcgggatctt
     2821 tacgccaggt tgcacagaaa aagataagcc atgagcttga ttttctccat gataaggatc
     2881 ttgtcctaaa atcaccactt taacttcttc ataaggagtc aattccaatg cttcatagat
     2941 atgatacata tctggatgga tcttttggga cgcatattct ttttttaaaa attctcttag
     3001 atgcagataa taatcttttg aaaattctga ggacaaaact tcttgccaac tgttatgaat
     3061 gatttctttc aaaaactcca cctcctaata aaaagaataa ctcacatttt cttcaatcac
     3121 aagggacaat cttcaccctc agcacttgat taatatggta aactaggaac aagaagcaga
     3181 aaggaacgat aatcattatg atcaaattaa ttgcatcaga tatggacggc acattattag
     3241 atgcccatat gagtgtttct cctgaaaatg ccgaggcgat acgttttgca aatgatgccg
     3301 gtgttgagtt catggtcgct actggtcgaa actatcaaga agctcgcgca gctttagatg
     3361 aggtaggaat cgattgtgcg atgatcactt tgaacggggc acaggtattt gataaagaag
     3421 gaaattcatt gtttaccgtg tcgattccaa ataatcaagc aatctctgtt ttagatattc
     3481 tagatgctaa cggtatttac tatgaagtag caaccaacga tggtctttat tctgaaaatc
     3541 aagcgaaacg aattgaaagt tttgcttcca tggtggctac ccatttacct caccttacgt
     3601 ataaaatggc gattgccatg gcctcagcta agctggagtt attgcacatc acatacgtgg
     3661 acagtattcg tgctattttg gaagaaaaga atctggaagt tttaaaaatt atttgttttc
     3721 atacagaagg gccagctgta ttaggtccag ttggaaagca gatttctctt ttaggcgatc
     3781 tagccgttac ttcctctggt caaaacaata tcgaagtgaa ccataaaaac gctcaaaaag
     3841 gaattgctgt tgcccatgtc gctcacgagc gtgggatttc tttagaagaa gtcatgacga
     3901 ttggcgataa cttcaatgat gtcagcatgc tgcaagtagc aggagtcagt ttcgcaatgg
     3961 gaaatgcaga aatcgaagta aaagattatg ccaaatatac aacggacaca aaccttgaat
     4021 ctggcgtagg aaaagccata ttgcgcgcga ttgcagaaga cttgtagttt tatctagaaa
     4081 agagggatat agatgtcaga atttttcatt caggaaaagc agcttggacg tgcaactcgg
     4141 acaattgtca aaaacgaaaa aggacattcc ctgtttttga tggttggtcg ctggggaaca
     4201 aagggcgatg cattgtctct ttatgccatg aatggcgatc tcgttgctca catcaagcag
     4261 ataagcctga cattcggtac gcgttttgag ctgtataaag agttcgaaaa ggtgggaacc
     4321 atgcagaaaa tattcaattg gcccggtgac ttttattata ttcgtagact tcactggaca
     4381 gtccaaggtg atatctataa tcatcaatat caaatccatc atttcaatca gcaaatcatg
     4441 aaaatggata aagcaacact tttttcagga gattactatg tattggatgt ctcaaatgat
     4501 gaagatgctc cagtttgtat ttgtattgct gcggttttag attactggct ctaccgtaaa
     4561 gacaaaagtc gcgataagcc tgtttcttac cgacttaact taagttaaaa agatgtacct
     4621 gaaatgcgga aatacgcatt tcaggtacat ctttttagat attcttttgc atatgatcat
     4681 aaagatgacc actgatcatc cgctgaccga caactttgaa gtcatggcgt tcgtataatt
     4741 ttttagcctt tggatttttt tcatccacac ttagaccgat gacggagcga ttagctttct
     4801 tcgccaattt aggtaatgct tctaataatc gcgaaccgat gccttgtcct ctaaaatctt
     4861 ctgatacact gatagagtcc aaataccatt cgttgggaaa cgtttcttta tcaatgaaca
     4921 aacgtgtaga cgcatctagc tgatatttct ctaagatagg taccagtggc tgatcgatga
     4981 ttgattcttc ttcatctgta taaccgtaag caactcctgc aactttaccg tctatttctt
     5041 ctacgatacc tcgtgaataa ctgtaacgat aatttggatc agtgattgct tcttctaaaa
     5101 tctctaaagt cttttgttcc cctgtctgct tcaaaaatgg cagttccata tcttttagaa
     5161 tcactaagat caatggggcg atttctggag catcttcttt tctcgcaaaa cgaatcatag
     5221 tttcctctcc ttttctccat tctaacatgc agaaaaagaa atcgcaaaat cgttatttta
     5281 aaaaatcttc aatcttttca gtaatgaaat aaccatgagt aacgattctt gccgcttcag
     5341 ttcggcaata agggcagacg actggcattt ctttatcatt atgttcgact acattattga
     5401 cttcttcgta tgcaaatttt tttccgcaat tagggcattt cattttttcc atgttatctc
     5461 aacttccttt catctttact ctcttagctt actcttatat tgtaaaaaag agaagcaata
     5521 tccttcttct atgcagatat ccatacctga cggtcctgtt ctccatctat taatcggact
     5581 ttataataag gacgttcatc tttgactaga agtgtccact ctttcacttt tgcttttttc
     5641 ccgtagtagc tgactgcttc cttcatcgct gcttgcggtt gtttgaactc ttccgttttt
     5701 tctatatcaa aaagatgttc tgcggccttt tccgtgtcat ttcgtacggt tgttttccca
     5761 gtggatggat tgactgctat gacttgtcct tcacaaaaaa actggtattc atatgttgca
     5821 ttcgtaggat tctccaaatc tttttcaact gcaaaatgaa tagcagatat gttatgttct
     5881 tttgtttcgt tttttacgat tgctgctgct tttttcaatg agatccgata ttcctggtca
     5941 ctaatatcat ccatgtgaaa agcggcattg agcagtcccg tcataccggt aatccctatt
     6001 acgatgaggg cagcaacacc aaacatcatt tttttcattc ttcattctcc tgtttctttt
     6061 ttttaaagaa tatcaagaaa tgaacgaatg aaaaatcgaa tgaaacgaaa aaaaagtgac
     6121 aattttgtta tctttattcg tgcagttcta atggcaatcc gtctggatca aagaaaaacg
     6181 tcattttttt tcctgtaaag gtatcttctc taatcggttc tgattcgatc cctaaggact
     6241 gcaattctgc aattgtttct tcaatatcgt ctactttgaa agcaagatga cgcaagccac
     6301 aagcttctgg atagtttggt cgttgtggcg cattagcaat tccaaaaatc tccaattcag
     6361 cacttcctag tttcagatct aatttatagt catctcgttc tttgcggtaa ttttctctga
     6421 ttacttcaaa tcctaattta ttaacataaa aatcacgtga tttctgataa tctgaaacaa
     6481 tgatggctat atgatggatc gttgtaaggt tcatatgagc actcctattc ttcgtttaag
     6541 ctcggatcta tattaatctc cagttccatt gtaaatgtgt cagattgaaa aaaatagctg
     6601 tttttccttt ttataagaaa aaaggcaaaa aaaacagaaa gtaggtcctc caagacactc
     6661 ttcctgttcc caatataata aatgaaagta agaaattata ttttcaagaa aacaaacaga
     6721 aaattatcat aacacaaatt atttataatt gaaagactag ttttttttca agaaaagcat
     6781 acaatagatt gtgttagtaa atacagaaca cactacattt agtgtcttaa ggagtcctca
     6841 tacatgaagt catattttca gtttttagct catcagctaa aaggctggcc gcaacaaaat
     6901 tattatcttt tctttttcag tcttggctgc caggtcatga ctcttgtcaa tagtcccatc
     6961 acttggctgt ccgttattac atttatcggt accacgcttg gtgtattgtg tattttatcc
     7021 atcaatgcgg caaaatcagt caacggtatt ttaggtattt tgtctgcgat ttgttttatt
     7081 atcgttgggt tttcagcaaa gaactattta agtatcggtg aacagattgc ttatatgatc
     7141 acattggata tccccgtatt gttaagtaaa gaatggaatc ataatatggc ttcaaaaatc
     7201 cgaaaattca ctggaaaaag ctggtttatc gcaatcatca gtactttcct tgtctatctt
     7261 gtttcaggat tcctgattgg tcgttataca gatgatcctc ggccgtgggt agatgccatt
     7321 gctttttcta ttagcttgac tgctggtatc atcagtttct tgcgttacaa caaccaatat
     7381 tactggtggt tagcttcagg gcttgctcaa atggtccttt ggtatatttc atttacgcat
     7441 ggatctgcaa gtttggcgat gttcgtgaac agctctgttt acttgctgaa tgatgtcctt
     7501 gcatttaccg tttcaccttg gtataaccaa aaagaacgtg aacgtatgca aaaaatggaa
     7561 gaagaatact acaacgcaca acaacacgca taaaaaaact gagcgtggga caaaagtaaa
     7621 gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaatcagaag caactccttc
     7681 ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt
     7741 cttggagtta aacgcttctg tcccagcctc tattttttat agtaattcct tcagatactt
     7801 tcttgcctca tcattgaaac gcagtgattc ttctaacttc tgataatatt ccaacgcttg
     7861 tttctgagct tcagtaaaac gataatgttt tcctttccat ttgtcgattg tctcttgcac
     7921 atttccagat tggatatctt tcactactag ttgccatttt tcttttgaga ctgtacggtc
     7981 tttcttatca gcagattgta tagcttctcg ttctttacgc aatactttta aaaaaccatt
     8041 tctgactatc cgaggaatgc tgtcgaagaa cccttctgta aaaccatagc gacaatattg
     8101 cttccaaata aatagataga tttttggttg gatgaatcgt tggtttttcg caaaatattc
     8161 catctgagca gtgacttctg gtgttcgttg acgttttgtc tttctcaaca cggaaataaa
     8221 tgtttcaaaa taagcattag gatgtactgg cacttcttca tatacgatgg attgccaatc
     8281 tttacgtaaa aatgcgcgat agttgatcaa gaattcttct acatccttta ctaattcttc
     8341 tgcaaagtcg tcaaatagtt ctttttttat ctgtcgcttt tttgactgcc cattgatgtg
     8401 ttcacttatt ttcttgattt cattttccaa tgcttgataa tccgctatgt aaatcggcat
     8461 ttctacttgg cgaaattctg cgagccgctg agctaatttt atatccggta aatatggctg
     8521 tttcatccga cgattttggt acaagacaaa acagtatttt tgccatagtc cttcaggaaa
     8581 atcgatattc ttctgcttaa gccataataa ttcgtctaag gccagatcaa cttttgtcat
     8641 tccctgatgg atcgaagctg ccactgtcgg agagacatgc aggtgatcgg caaagtggtt
     8701 gatgcctagt tttaggatct ttccggtttt tggcgattta acgatgtatt gcacttttaa
     8761 ttctctacca cactcacaat acaatttttg ctggcttcgt tttgttcgaa aaaacggatt
     8821 gatcttgaag tcagaaaaca cccaatctgt tgcagctaaa taatgttctt ccataaaacg
     8881 agagctgatt agatatcggc ggtgcttatc gactagttcc cgctgttttt tcgttaattt
     8941 tgcatagatc tcccaacgtt gactgcttgt gtattgttta gaaattttga tgggatagct
     9001 tttgctgatt tcttttggaa accattcttc aaagacggat tgccattctt ccacggctgt
     9061 ttctttcctt tctaattaca aaaagctggc tactaacata acgttagcaa ccagccagtg
     9121 tttcttgcta aattagtgag ttgttccggc atttccggaa atatcttctt tattttctac
     9181 tgctgcttca actgcataag tcaaagaaga agcaatgtct tctaaagaca tagaaggcgt
     9241 acccactgga cgatcgatca cttgatcggg tgagaatggc acgtggatga acccacctcg
     9301 aattgccggg aattgtgttt cgatcaaatg aagcaaacga tacatgatat cattgcagac
     9361 aaatgttcct gctgtataag agatgtacgc tggtagacca tgatcttgta cattttttgt
     9421 cattgctttg attggcaatg aggtaaagta agcggctgga ccgttttctt ctaaagccgt
     9481 atcgaatggc tgatttcctt cattatcagg gattcttgct tctgctagat tgattgcgac
     9541 acgttcgaac gagactgctg accgcccacc tgcttgtccg acacagatca cgacatctgg
     9601 ttggtgctgt tggatggctt ctttgactac ttgactgctt ttttcgaata cggtcggtac
     9661 ttccactttg atgatttcag caccgctgat ttcatctggc atttttttta ctgcttcata
     9721 ggctggattc actttgtccc caccaaatgg atcaaaacct gttactagta ctttcatctg
     9781 ttgttcctcc gtccgattga tttaacatat ggttatcttt tatcatacca ttttccttga
     9841 acagcgtaaa taaaggaaac aatagtttaa ataacataac agatcaaact taatatattc
     9901 ttataaaaaa atagtgttcc actacaatgt tagaagaggt tgcgacaatt attctgtcac
     9961 aacccctctc ttctattatt taaatagaat catataagca atttggaaaa ctagcataaa
    10021 taacgcaata aacaactggt ttttgatcac tccgtatttg tctttcatct ccagcatcgc
    10081 tacaggcacg atattgaagt tagcagccat cggtgtcagc agggtcccac aaaaaccgca
    10141 agtcaatgca accattccta ttaacgctgg attcgctcca tgactaaata cgaatggtgc
    10201 accgatccca accgtcatca cggtgatggc cgcaaatgca ttccccatga ttaccgtaaa
    10261 tatcaccatg ccaagagcgt aaatgatgat gccgatgttc acatttcctt ctggaacgat
    10321 cgtacctact gcattggaaa tcactgtccc aactccagcg cttgtaaata ccgcacctag
    10381 agaagctaaa agcattggca acatgcttaa cggccctact gtgcccagca tatctgctgc
    10441 atcatctaaa aatgtacttg gtcgattatc tcgagaaaag aacatcaaga ttagcatggc
    10501 tgcaaaaaca ccgacgccca cgccgactaa agcacctaaa ttggtaaata gagcaaacaa
    10561 gatcgcaaag acaccgatac tcaaagctgg aataaaaatt ttcattccta gtttgtctga
    10621 cattttttcc atatattctt ttgatggaag ccggctttct cctttgctga ctttcttagc
    10681 aattgctggt atggtcatcg caaagatcaa gccaccgttc acgattgccg gaagccatct
    10741 tcctcctgca ataactaccc ctaagaccgt ccagaaaaag gctgacccat aacgatgagg
    10801 attggtttta tctgttaaat ttcttactcc agtgtaaatc aatacgagtc ccatcacgat
    10861 ataaaggatc tccagtaatt tatcgcctaa taaaatatca ggatttgtaa aaaagtccat
    10921 cttattgccc ctttcctgga taatatttct tcgctaattt cttttcttta agatagaagt
    10981 agacacttgc cacaactaaa gccgtgatag ccactggaac ttccactaaa gcaagctgtc
    11041 ccaatgttac gtcatatcct agatctttca gtgttgattg gactagcaac gcccctgctc
    11101 ctccgatgaa tagtacttgg ccaaagaacc agcagacatt ttccatagct gaagccatac
    11161 cttttagttc ttcttcatgc ttaggatgga cttctaaccc ttttgattcg atggtcccca
    11221 atgccatcgg taaaatgatt ggacgaacaa atccagcaac tccaccaaaa ctcacgttaa
    11281 aagccgcaaa gatcattcga aagatcccat aaatatctac aatcatacca gcagatactt
    11341 tcttgaatcg cttgatcaag gaagccgctg attctttcag accatttctt tcaagcgttc
    11401 ctgttgccaa cataatgatg ataaaaatcg ccatcccgcg attgtttaca aaacttgtac
    11461 ctaaaagttc taacattcct tgaatgccaa gaccgccagt caaagctgta acaactaaag
    11521 caaccattac gatcaatatc gagtctaatt taaatgcaaa cccgagaata acaatcaaga
    11581 cgcctaaaag gctaagataa ttttccatat attttcctcc ataaaatgac aatatttttt
    11641 taattataaa gcaaaacaca taaaaaatat agttgttttc tcacgaaata gacactattt
    11701 ttaaatattt ttcgttattt ttttatcaca aaaattatct tacttattgt aaggtaaagc
    11761 tttttttaat ttattgcccg ataatatata gaaataaggt ctagttactg ttttttttaa
    11821 atgattaata aataacaact tctttctatg aaattgcaaa gcattcatct tcttttccgc
    11881 taacgatata tccgtcagtg cttggtacac tttactaaaa agtatgagat tgccgatatc
    11941 tggataggtg tgctatacta cagaaaaaga tatacaatct gccaccgggt aggataaaga
    12001 ggatgatcat tccattaggt ctttgaaggg atgattttcc tctttttgtg tattttttta
    12061 tagttaagga ggaatgtaag tatggcatgg atggaattaa tcatagcagg aattttggaa
    12121 gtattctggt caacgatgat gaaatggagt gatggttttt ctaaaatcaa ttattctttt
    12181 tacacagtga ttggcatgat tgctagtttt tactttttgt caaaggcaat caaaagtttg
    12241 ccgatgagtc tctcttatcc tatctggaca gggattggcg cagttggttc ggtcataatt
    12301 ggtgtggtct tgtttcatga taagctgaat ctttccactt ggttctttgt ggggatgcta
    12361 ctcatcagta tcattggcat caagattact agcggccact agacacttgg aataagtact
    12421 gatcagaaaa aataagcctt tttatttaat attcaatttt tttatgaaaa aataagaaat
    12481 aagtccaatg acctagcgac ttctccactc atcaggtgta tgcttgtact atctatagaa
    12541 aaggagtgaa tatttttgaa aagtctttcg gtccaagaat tatccaagaa ctttggtcac
    12601 aaacaagccc tcagcgatgt cagttttagc ttatcccctg gcaaaatcgt tggattagta
    12661 ggtcctaatg gcgctggaaa aacaacgatc atgaaagcta ttttaggact gatccattac
    12721 tcagatggaa caatctgtat tggtgaaaat gagatttctc cctctagtca taaagggttg
    12781 gaacaagtag gagcattgat cgaatacccg ggtatctatc cattcttaac aggctatgat
    12841 catctgaagt tattctctga aaccaacgat gtatctgcta tcgacactat cgtgaatcag
    12901 ctcaagatgg aaaagtacat tcacaaaaaa gcgaaatcct actcgttggg aatgaaacaa
    12961 aaattgggaa ttgctcttgc cttactgaac catccgtcta tcgttatttt agatgagccg
    13021 atgaacggtt tggatccaca agcaacaaga gatgtgcgaa aaatcattac cgacttggct
    13081 actcagggca cctctttctt gatctccagt cacttactca gcgagcttga aaaaatggtc
    13141 gatgagctga tcctgatcaa tcaaggaaag atcatcaaac aatgtacaat ggctgaattg
    13201 aaccaaacag cgcaagattt catcgttatc tctacaacaa atgatctagc tgctcgaacg
    13261 atcttgacag aagctggata tactttggaa gatcctgata aaatcaagct tttaaaagaa
    13321 aacgaagaat tattaggaac cgtgatccag ctattgtctg cacaaaatat ccatgtgaca
    13381 gacgtccagc atctgacgaa tgatttggaa tcttctctat tggaaattct ggattcagta
    13441 aagggggaag aagaaaaatg acattgcttg taaaacaaga attatttaaa ttgatcaaga
    13501 aaaagtcaac tgctgtttta tctgttctat tagttgtttt gctcatagga acagcacttc
    13561 tagccaaaaa atacacgacg atcattgatc cagttgaaat gactgctcag ctttttagtg
    13621 ccacatcttg gattgtcttt atcatgattg cagctgcaag cacgatcatt tcgatggaag
    13681 cacaatatgg tacattgaaa aatctccttt atcgaaagta ttctcgagga gaaatattag
    13741 taagcaaatg gatcacatta gttatttatt ctgtttattt atacctttta gctattattg
    13801 tcactgtgtt gatgaaactg attcttttcc cttcgatttc cttcacggag aaggtttcta
    13861 ctggtcaaac actgatccaa agcttgttca cttatacttt gggtagttat atcggtttat
    13921 ggctgatttt aagtttagtg ttgatgattg cttgctttat caatagttcc ggcgcatcca
    13981 tctcagcagg aatcgtcttt tattttgctt cgtcaatcat atctggtatc cttattgcac
    14041 tcatccaaaa atgggagtgg atcaaatgga atccgatcag tatgttgaat ctgcaaaacc
    14101 aaattggaaa cgaagagatc atgaagcaat tgactcatct ttctactaat caaatgctat
    14161 tcggtaattt agcttacatc gttttgttct tagcattagg atatctcgta tttaagcgaa
    14221 aaaatgtatg atgactcatc aaaaaagagg ctgtgaaaga gacgttcagc cttattgccg
    14281 attggctgtc tcttaaacac cgtttattct gaaaaaggag ctgtgacaac atttgtgtca
    14341 cagctccttt cctagtatgt ttttgaataa acgacaggct cacaacctct ctttatactt
    14401 cttttatact tctatacgtg aagcatccca gtggcaaata aaaccattgc aatcactccg
    14461 accatcgtca cacctataag taaaatctta tctaagtgga tatgttgtac aggctcgttt
    14521 tgctccttac gtgctttgag gtaaaaataa atgcctggca agaaaagaac aagaacaagc
    14581 attacttcat taaaggctac gactagcata gcccatactt gaaaggcact tgctaaaagc
    14641 cctactaaaa attggaccca ttctttccgc tgataagaaa gtttcatttg gtacaatcct
    14701 aagaaaagcc acgtgaagaa aatagctgct gtaccaagtg tgtaagcaaa gttataagca
    14761 ctgtcagtga ataataatga aagtaaaaag acattcgtac ataacgtcgt caatacgata
    14821 gcataagttg gtgccccatt tttgttgagt tttccccatg ctttaggcaa aagtccgtct
    14881 tcagccatgt ttcccattgt ttcaatcggt aagatcgtcc atgagagcca aacaccaact
    14941 aaagaaatga tcaaaccgcc gttgatcacg actgctcccc atttaccaac catcatttca
    15001 aacacatagc ccatagcagg ctgcgccttg atcgtttgca gctgattcat cgtcattgtc
    15061 ccatatggaa gcatggaaac caagacgtaa ataataatca agccgaatat ccctagaatc
    15121 gttgctttcc caacatctga ttgttttttt gctcgtgaac ctacgacaga ggctccttca
    15181 ataccaacaa atacccaaag catcaccatg atgcaactca atacttgact ggaaaacggg
    15241 atcggctgtc cattcgcatt caatataatg tcgctagaaa agttatcaat gaattgttgg
    15301 atcgaaaacg tgctgatagc tactacaata aataaaaata atggcactaa tttacagaat
    15361 gtgacgatag cattgacgaa actagcattt tcgactcccc gattcactag ccatgcatat
    15421 ccccataaaa agactgtact catcacgatc gatgccagat tttgcccacc ttcaaatact
    15481 gggaagaaat atcctaatgc gctcatcaac aatgttgcaa aagctacgtt gcctaaccag
    15541 catgtgagcc agtaacccca accgctgata aaaccagcga aacggccaaa accttcttct
    15601 gcgtaactaa agatcccgcc ttttaaatct ggacgttttt tattgatatt attgatcgac
    15661 aaggagagca tcaacattgc tgttcctacg agtacccaac tgaaaatagc tgggccgcca
    15721 gccgatccgg agatgtccgt cataatacca aatacgccac ctccaatcgc tgaaccaatt
    15781 actaaggcag caagtggaat taaaccaatt ccttttttct gttgctctgt atccatgtaa
    15841 gcatcctcca aaataaaacc gcttttatca aaaagtataa caaacctttt ttatttttac
    15901 ttaattaata aaaactttaa tagaaatata attctaccgg cttttttctc attatacaag
    15961 caaagcgcct agtccctttc caaattacac caccaaaaaa cttaaaaaag atgctatact
    16021 aaaaggaaag aattggagga tgatccatga acattttata tatatcgatt tcaggaaata
    16081 cgcgtgcctt tgccaaacat ctagcagaat atgctgaaaa aatgcatgca gaagatcctg
    16141 tcaatcctga agttacttta aaagaaatcc atgaaaatag tgattttacg aaagaaacag
    16201 aaccattttt tactttcgtt cctacttatt tagacggcgg aaatggttta gataacggtg
    16261 atacagaaat cttgaccgaa acgatgcgtg aatatctgga acatgaagac aatcataaac
    16321 tttgtcttgg tgtagtcggt agcggaaata aaaacttcaa taatcagtat tgtctaacag
    16381 ccaaacaata tgcacaaaca tttggctttc ctttcttggc agattatgaa ttacggggaa
    16441 caccatctga tgtgacacga atttatcaga ttttagcaga aaacaataaa taataaaaaa
    16501 gaagaagctg aaaaatagat ttcagtttct tctttcgttt ctattgtttc tatcatatta
    16561 agctttgaca tccatcacag gtgcttttgc ttttgcttta cctgtttttt ccaagacaaa
    16621 gttttttaca cgatccatat tggttaccaa gacgatcatg gatgtttctt tgcctgctgc
    16681 tttgatcgct tctaagtcag cttctgctac aagcgtatca ggtgtcactt gctgcccttc
    16741 tgttactttc acatcaaatg gcgtaccgcc taagtcaact gtgttgattc ccatatgaag
    16801 caagatttct aaaccatttg gcgttgtgat cccaatagcg tgtttcgttg ggaagacaga
    16861 gctgactacc ccagtaactg gagaatagat atgtccgttt tctggttcta caaagtagcc
    16921 ttcacccatc atcttttgtg caaaaactgg atcacttgct ttatctaaag aaacaactgt
    16981 tccgtcagca actgatgaga ggatagtcac actttcagct gaagtttgag gtactacttt
    17041 ttcaggcact tcatttactg tggctgtttc agtcgctaat tcttcttttt ctaaacggtc
    17101 tttaggaata ccgaagaaga atgtagcaac gaagccacca gcataagctg ccaataatcc
    17161 aaggatgtag cctaaccatt taccgtcaga gatcaatggg atcaaagcgg ctcctgaagg
    17221 tccaatagcg atagccccga catttccgat cattccgatc actgccccgc cgatacctcc
    17281 accgatacaa gcagtaataa atgggcgtcc tagaggtaat gttactccgt aaatcaatgg
    17341 ttcgccgatt cctaggatcc cgactggtaa agctcctttg atcatttctg ttaattcttt
    17401 atcttttttg cagcgaatcc ataatgccaa tgctgcaccc acttgtcctg caccagccat
    17461 cgcaagaatt ggaagcaata gcgtacgacc agtttcatca atcatttgca catggattgg
    17521 agtcaaaatt tgatgcagtc cgaacataac catcggcaag aataatgtgc ctagaacgaa
    17581 gccggcaaat gctccaccaa cgccaagtac ccagttgatc ccaccgacca tgccatcaga
    17641 aatgaaacct gctaaaggca tgatgaagaa aatttcaatc agaccgatgg caatcaatga
    17701 aagagttggt gtaacgatga tatctaccgc atcaggtact actttatgca gttttttctc
    17761 aacgattgac aatagccaaa cagcgaagat gacacctagg atcccacctt gtccagctgc
    17821 aagagcttca ccagtaaata agtttttcag tggtgcttcc ggattcatcc ctgtcagcat
    17881 cacaacggcc ccgataactc caccaagagt tggtgttgca ccaaaaactt gtgcagcatt
    17941 gatacctgta tagataacta ggtagctaaa catcccgttt ttcaagatgt tcatcacatc
    18001 gatatattgt tgccatgttg ctgcatcaag tgttccggct gtgactaagt ttgctaaaac
    18061 tgcggcaatc cctgccacga tcccagtacc gacaaatgca ggaatcattg gcacaaagat
    18121 attagaaatg tcttttaata ccgatttgat tttagatggt ttttgtttcg atttttgtgc
    18181 cgctttcatt tcttgtgccc gttcgtttac acggtctttt ccagatgctg atgaatttgc
    18241 tgtagctgta cttacagaag aaagtttttc tcctaatttc acgcctgcca aatcaaccat
    18301 ttgctgtgct actttgttca ctttcccagg tccgatgatg acttgaagct gttcatcttc
    18361 tactacacca agcactcctg gaatcgcttt taattcagag acatttacaa gatcatcatt
    18421 gatgatcttc atacgcacac gagtcatgca gtggacaatg ctttctacat tgcccatgcc
    18481 gcctgcccct ttataaattt gttttgccag ttcttccaaa gtcagttcgt tttttgccat
    18541 ggtcttttcc ccctcataag gtccctttta caaatccgtt tgcttcagct aattttgctt
    18601 gagcttcctc tttatcacta tttgtcaaaa tcatgacgat agccagctta acattttgat
    18661 ctgcttcttc aaagtacttt tcagctgttt cgtaatcagc ttctgtcgct tgcatgatga
    18721 ttcgttttga gcgttcgatt agtttttcat ttgttggttt gacatcgacc atcaagttat
    18781 tgaatacttt acctatacca atcatactga tagtagaaat catattcaag ataagttttt
    18841 gtgctgttcc tgacttcaat cgagtagagc ctgtcaaaaa ttctggacct gcatcgactt
    18901 cgattgggaa atccgcgtgt ttactgattt cggcttgttt attgcaagaa atcgttcctg
    18961 tgacagcacc gatcgatttt gcgtaatcta atccaccgat cacatatggt gtacgaccgc
    19021 tagctgcgat accaataact atatcatttt ttgtcaaatt cagcttgatt agatcttctt
    19081 gccctagctc ataagaatct tcggcccctt cgacagctac agtcattgct tcttctccac
    19141 cagcaattag acctatgacc atttccggtt ctacaccgaa tgtcggtacg cattcagcag
    19201 catctaacac acctagtctt ccacttgttc cggctcccat ataaatcaga cgtccacctt
    19261 ttttaaagga ctcaatcgtg ttagcaatca ctggttcgat acttgtcagt tctttttcaa
    19321 ccgcagcggc cactttctga tcttctttgt tcattagttg aacggcttga gaaatactca
    19381 tctcatctaa tccaaaagtt tcttggtttc tgcgttccgt tgttaggttc tctaaattga
    19441 ttttgctcat ttttatcttc cttcttttct tataaattga tagttcatac cagctttgat
    19501 ttggttgatc aatggtagct cttctttgat aactttagct actacattca ctttttcatc
    19561 cgctggcaaa tcatatttgg ttaattggat ctcacccata tagcgcaaat acttttcgtt
    19621 atctaaagta atagaccctt tcatacgcgt agctgtattt cttgctggga taggcggaat
    19681 ctctcgaaaa cgtgcatcta cgcttcgtat gacatctctt gcatcgtctt gccgattcgt
    19741 atgcttccct aatatatatt cgtaaaattc ttcatctaca ggttccgtat ggagcaagac
    19801 tgtctgttct ttttggaaag aagcaaattg ctcctgaacg ttttctgaca gacctgcatc
    19861 tccgatgtaa acaatatccg tgttacttgc taatagatct agagcgcctg caagaggatg
    19921 gatccctcga tgttcttcca aagttggcaa tccttgatat agtgggcctc tcaactctgt
    19981 atctcctggt acaaaacctt ggacaaggag accttgaatt ttgagccatt gatttttttt
    20041 ctgataccat tctttgtcca gccctgtttc gggacgtgga taataattat gccaagcttc
    20101 aatctgtgag aaatcagctt ctgcttcttt caattcatcg atatctttag gcgtgatcgt
    20161 gctagcattc aaactgattt tcaattgatg tgaccagtcg gcaatctgct gattggaaat
    20221 atgataatcc attcttaaac ctgttacacc aattgctttc aaaggcgtta gatcttctaa
    20281 tgaaaaaccc gctcgttcca atgcctctcc tgaaatatcg accatcaatt ctagtttatt
    20341 ctttttcgct tcgcttccta gttcgatcaa tcgcttttga tatgccgaaa catcatcttc
    20401 tggtatatgc atggaagtga aaataccgac aaagccattg gatgccattt ggcggatata
    20461 ctgtttcttc tcttctgaca aagaatcgtt cataaatatt gaaaagccaa acatattatc
    20521 cctctttttc tgtatccgtc ttcatttcta tgactagagt atatatctaa agaaacaaaa
    20581 tttcaatact tttattaaaa taattaaatt tcatttctaa aggttgagtt tatattatca
    20641 aaagaattgt cataacctaa aaaaggcact gctaagcaaa agcagtgcct tgaaatattt
    20701 tatttaaata acacaaaaac ataattactc ataagaaacc aattctgtat tatctaccat
    20761 gaatgtaaag aacccctcat taggcatcgg atgaaaataa gcattgatca ctcttatcaa
    20821 ggcattatgg caaactaaca gatagacatt ttcttcgtct tcgatgcatt ctttcaataa
    20881 aggaacgatc cgtgcataca catctaagac tgactcccca tttgggaatc gtacagcgaa
    20941 cgctaaacgt gctttttgaa agttctcatc tttgcttggc agtccatcat aatccccaaa
    21001 atccatttct accaatcgtt catcaacggt cagcggcaag ctgagacgat cagcaactgc
    21061 ttgtgctgta tctcttgctc gttgaagagg ggaatggatg attttcgtga ttggaacagg
    21121 caaatctact accttttctg ctagtttctg tgcctgcatc tgtccttttt cagtcagcgg
    21181 cacatctgcc cgtccgcaaa ctaatccttg tgcgttccaa gttgtttctc cgtgtctcgt
    21241 tacataaagc atgtgattct ctccttcgtt ggcaaagttc cccttaacca taccttgttg
    21301 ttcttgagat tacaagggtt agattctctc aagagaaata atctcagtag atactttatc
    21361 caggaaggtt tgatcatgtt ccactagtaa catggcaggt ttcacgttca agatcagttg
    21421 ttctaattgt tcttgattga agacatccaa ataattcaat ggttcatccc atgtatatag
    21481 ttcagctggc tgtgataaag atttagccaa ttccactttt ttccgttggc ccatactcat
    21541 ctgctcgatc ttgttatgaa aaacatctct ttccatccca agctttcgga ggttgttcaa
    21601 aaatgcttgg tagtctactt ggtttttctc tgcaaattcc gccaacgttc ctcgattgtc
    21661 ttcataattt tggcttgcat aactaatgct cagatgtttt ggctggtatt tttctcctat
    21721 aaccttgccg ttgaatgccc ccagaagata atggataatg gatgactttc ctgcaccgtt
    21781 aggacctgaa atcgccaccc gctgatgagg ctcgattgta aaatgaattg gctcgaataa
    21841 cagattttca taccctaatt gaagatcttc tacgcttaaa agtcgcttat ggtgagacgc
    21901 ttggctattc atcgtcaaag aatcgatata ttcgatatct tttaatagtt tttctttctc
    21961 gctgatctgg gtctccatcc gattcacgat tgctttggat cgtttcatcg tccgtgcagc
    22021 atcagcaccc actgctcctt tattcactcg tctagattca gtatcgatga atccgacttg
    22081 tttctttgtt ttatctcctt ctcgggaacg agaccattcg gctttttcag ctgctgtttt
    22141 ctttagccta ctgacttctt ttttcaattt ttcattttga gccatttcaa attcatcacg
    22201 aagttttttc tgttcttcat agatagagaa attcccttga taaagttcca gttgactttt
    22261 ttcgattgct aaaacatggt ccactacttc atcgataaat ccccggtcat ggctgaccac
    22321 gatgaagcct tgttttttct ttttcaaata agccgctact tgttttctac cagagatatc
    22381 caaatgattc gttggttcat cgattaacgg gaaatgagtg tcatccacaa ataaaagtgc
    22441 cagtaggact tttgtcttct ctccccccga tagtgtgctg aattctctcc ataagatttc
    22501 aggatctgtt tgcatcaatt ggagctctct ttcgatttcc catatctcaa aatccgtaat
    22561 atcatttaac acgtaatagg ttaaacgttc tttatctttt gtctgttgcg ggaaataggc
    22621 aaattcttgc tgatggatta cttgcccttg ataaggtagt ttgttttgta gaatattcag
    22681 taaagttgtc tttcctcgac cgtttcgtcc gataagtcct agtttccatt gtgtgtcaaa
    22741 atttagattt gcttgttcaa ataataatgt gccttggctg tcgtagccga atgtcagatt
    22801 ttttatttcg attttagaca ttttccatca cctcgtaaaa aaagaccagt cctattttca
    22861 gaactggcta aagaggatgc aagggttgga tacacacaaa aagacccata catcgaaaaa
    22921 accaagccaa tcccgaaaat ctgcacactg cttccttgtc tccacaaggt acagtctgaa
    22981 tgcagctttc atcaatcgac ttaatttttc aatgcacgga tccctcactt tgtcctatat
    23041 ttgaaattac gatagcataa agaaaatgat tttgtcaact acttcgggag gatggtgaaa
    23101 aatctgctaa gtgtctgtcc taagattttt gttttttcta atgtttgcct gtgttccatt
    23161 gcttcttcta aacctattgg acccaaatga atacttaaga caattgggag aaaatcagaa
    23221 agtccttcta gctcttggtc tctacttccg caaatagcga ttattggttt tcgatatttt
    23281 tttgcaagca ctgctacacc gtatggaact tttccgtgga tcgtttgctg atcgatccgc
    23341 ccttctcctg taatgatgac atctgcttgt tcgatctttt tttccaatcc agtgatttgg
    23401 ctgactaaat cgaaaccaga aaccatcttc gctcccaaca aaaaaagggc tccaccaata
    23461 ccgcctgctg ctccacttcc tggaatatca tctaaatcca cctcccagaa atcttttatt
    23521 tgctgagcaa tctttcctgc ttgtttatct aagaaagcta gcgtcgtatc tattcctcct
    23581 ttttgtcttc caaattgcca agcagctcct tgttgacctg tatatggatt ggtaacatct
    23641 gcagcgataa tcaattttac tttagaaaca agaacgtttc ttcgatcaaa atgaagtttt
    23701 ttagtagata ataaaggatt tccttctgtg tacccttcta aagaagcacc caaggattgg
    23761 agtaatccta gaccaccatc gctacacccg cttcctccta aagtgacaat aatttgttct
    23821 acttcttgaa gcagtgcatc tttaataagt tctcctaagg cataactgga agcaagtcgg
    23881 atagtttgat ccgttggttt atcaagtaga tctaacccca atgcttgtgc agattcgatg
    23941 actgcggtgt tttttccttg ccaagttgtc aataaatagc tgatttttcg ctgacggaat
    24001 aagagatcca tcccatcaat ggtacgccat gttcccctga gcgcgtaata gattgcttct
    24061 gcagtatttt ctccgccatc tgcaatcggc acattgattt ttcttccttt acgatcttcc
    24121 cagccattta gaactgcctg atttagttca aatgaagaag cacttccttt aaatgaatca
    24181 attgccgcca aaatatccat ctgtatccct cactttcttc ttaagtaaga cacttttttt
    24241 catttttttc aaacaaaaca atgctatttt cttccatttt tctatatact aaaagggaac
    24301 tcattgaata aaggagagaa aaaacatgaa aatcgcaata gctggggccg gagcaatggg
    24361 cagccgaatc ggattgatgc tacatcaaag cggaaatgag gtcctcttga tcgatcgttg
    24421 gcctgctcac attgaagcca ttcgtacaaa tgggttgatt gctgacttta acggtaaaga
    24481 agtggtcgca aaactgccaa tctattcacc agaagaaatc atcgaaagca acgaacacgt
    24541 ggacttgatc gtagcattaa caaaagccaa tcaattagat gatatgtttt gttcgatcca
    24601 atccattatt actgacaata cttatgtttt gtgtttacta aatggattag gtcatgaaga
    24661 tgtactggaa aaatatgtgc caaagaaaaa tatcttattt gggatcacga tgtggacagc
    24721 tggtctagct ggtcccggaa aagtcacatt gttaggtgat ggagaaatcg aactagagaa
    24781 cttggaacca gaaggcgaag catttacaaa aaaagttgta gaagtctttc aagaagcaaa
    24841 tttaaatccg atctatagcc ataacgttcg ttattccatt tggcgaaaag cttgtgtaaa
    24901 tggaacattg aatggtttat gcacgatttt ggattgtaat attgctgaac taggcgcgca
    24961 aaaagcggca gagagcatgg ttcgaacaat cgtatctgaa tttgcttcta tcgcagcaaa
    25021 agaaggaatc atactagatc aagaagaagt gtaccaacat attgcttcta cttatgatcc
    25081 tgacaacatc gggttgcatt acccatctat gtaccaagac ctgatcaaaa accatcgttt
    25141 gactgaaatc gactacatca atggtgccat ttggcgaaaa ggacaaaaat atgatattgc
    25201 tacaccatat tgtgcctttt tgacccaatt agtccatgca aaagaaggaa tccttggcgc
    25261 agaataaaag gctctctgat gagaatcagc tgaatcagca gctggttctc tttttttaag
    25321 cccaataaaa gatcaaaaag aaaacacaac tctcatgatg ccataagaat tgtgcttgtt
    25381 tgtttcagta cataatctct tcaaaatgta ggttttttat ttgttttcaa cagaggagag
    25441 gtcatattcg tattgctcat aaatagtgga atcgttagtg ataaatcgtt ttgcccaatt
    25501 ttctgcatca taaaattcca atagctctga taggattggc ttaaatggtg tatgcagaaa
    25561 ttccggtaat tcatctaaat cacgttctcg aagcaaaaaa atattttcag gacgataaaa
    25621 atgataatga gcaatcgctt gattatccgt cactagtttc ttttgaaaat acatggattc
    25681 aaatgtccgc aaagtcactc cttgctgacc ctcccgcaac agttccagaa tcccgcgcga
    25741 ttcccctgtt tttttcaggt actcttcata agtaagatat ccaggttgtt cattccctcg
    25801 atgtggaaga atgaaatagt tggtcgtaac atctcttttt ataaattcct gcttatagct
    25861 ttctgctcgt tcttttctcc cattattcgt tgcaccaaaa aatagatccg tctttggttc
    25921 gctagaagaa aaattcatat gtttcgaata aaatgaacta ttatgtttca gcccaagttc
    25981 ttttgcttcc aaaaaatcaa aatgataaat ctcatcgatt gccggttcat cttgtgcctg
    26041 cttcagcatc gcctcgtgtt ctgctttgaa gtgattccaa aaaaataaca caatttttgc
    26101 attggtaaat gggcgcacgc gtcgagccat ctccatatca aatccataat ctaaaaaaac
    26161 gatcagctga taatttgcca actgttgtct agctgattta atgaattccg actgtagtaa
    26221 atcttttccg gaccaaacat ctaaagagcg gatggaaggc aaaaaataaa gtgatttatt
    26281 ttttgcaact agcagtacgt catttttgtt catcttttcc gcctcacttt ctctgtcact
    26341 ttttcctatg atagcctgtt taaatagtaa aaagccagta aaacgtttcc tatatttgta
    26401 tgaaaattta acatttgtat gaaaatatga tgaaataaat cattcttcta ttaaaattcg
    26461 cctaaaagtt ttctgaatag ttgtaattgt ctgaaaattt gttatcatta gtcattgtta
    26521 aatattgcta tttattctta atataaattt tgattgtgaa tttaaaagaa agaggtcatt
    26581 tatggaagat aaaaaattga aaaaacaact tagctccaga catatcacca tgttagcttt
    26641 aggtggagca atcggtgcag gtctatttaa aggaagtggc gaagcgattg ggatcgccgg
    26701 cccttctgta ttgattgctt ttttgattgg tgggctgatt ttatatatcg tgatgaaagg
    26761 tctgggaaaa atcgttttaa gcggtggcga cacgcatcac ggattatctg gtttgatacg
    26821 cccttatcta ggtgctcatt ctgctgattt tactgattgg gtctactggt ctatgtggat
    26881 tatcaatatc atcgctgaag ctgttgcagc tgcttccttt ttgcagttgt ggttcccgca
    26941 cattccagcc tgggtatttg tttttatgtt ggctgtttta actacgatca tcaatcttta
    27001 ttcggtacgg ttattcgcag aaacagaata ttggctggct tttgctaaaa tcagtgtcat
    27061 tatcttactg atcatttttg ctacttatct tgtcggtcag caaatgttgg gcacaggcgt
    27121 tttccctacc cttcagcaat tgactgacca tggtgggttt actcctcatg gtatgaaggg
    27181 aatcatcagt tctttgcttg tagttatcta ttcttacggc ggttctgaac tgattgctat
    27241 cacggtaagc gaagcagatg atcctaaacg tgcgattcct aaagcaatca aaggtgtcat
    27301 gggaagaatc atttcctttt acatcattcc tttgtttttg cttttgattc tttttccttg
    27361 gaatacttta gcaggcacga atgtcagtcc ttttgtgatg gtatttgaaa aaatgaatat
    27421 cccatttgca gcggatatcg tcaattttgt gattgtcctt gctttgttct catctatcaa
    27481 ttcgggtgtg tatgcatctt caagaatttt gttcttccgt ttgaaggatc gaaaaggttc
    27541 ctctagaaaa ctagctgttt taaacaagca tcaagtaccg caacgtgctg ttttattttg
    27601 taccagtgtt ctttatcttg gtgtagctct ttcttatttt gtcggtgaca aattatttgg
    27661 ctatctggca ggttcccttt cgtatacagt attattgatc tggatcatta tcagtgcggc
    27721 atcctttgta ctggcactaa gaaaaggaac attatgggat aaaggaatca gcttttttgc
    27781 attagctgtc ttaggattga ttttccttgg tatcctgttc acgaactcca ttggggtaac
    27841 tgtattgact ggcctgcttt acctgttcat ttatttcagt tatcaaaaaa agaatgatgc
    27901 gttcgtattg acgaatgaat gaaatcgata gaaaatttta ttttatttat gtggaataga
    27961 tggagctatt gaagaaagga gttgtgacaa aagtcttgtt gcaactcctt tttccgaata
    28021 aacagtctca tgacctcttg gtaaacggtg ttcaaaaacc ctctgtgcaa aataattgat
    28081 tgttctccat gctcatgaca tgatagaaga aaggaatgaa aaaaaggagt ttgttttaaa
    28141 tgaaaaagta ttggaagttt ttgttgatga tcggtgtttc aacggtagtt atgtttggta
    28201 tgatgtactt gaatgtctat gcgctcgatc atatcttctt cagtcgtaca cgagtattca
    28261 tggcgttgat gatgggtgca gtcatggcag tcatcatgct gttgttcatg tggaaaatgt
    28321 atgaaaacaa aaagataaat gttatgatct tagccgctag tcttcttgtt tttgccggtt
    28381 ccttgtttat ggtacgaagc caaactactg tcaacgacac agcttggatg aaggccatga
    28441 ttcctcatca ttctatcgct atcctggcaa gtaaacgagc tgatttaaaa gatcctgaag
    28501 taaaaaaatt agcggaagaa atcattcagg cacaagaaga agagatcgac aaaatgaaaa
    28561 aaatgatcaa agaaatggaa tgaccattat ccgtccaaat aaaaaaagag gatgttccag
    28621 aagttatcag cttcaagaaa taagaagaag ctacttctgg aacgccgtga ataaggggtt
    28681 gtgaaaaagc tgccctgcat caagtaataa gaacagccaa acaaaaatag cttctcctat
    28741 ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt tgaacaccgt ttattaagag
    28801 gttgtaaaat cagcgttctg acctgagtat tagagagaca aatggaaaaa tagttcctca
    28861 tattttgcca ttttttgagc taataccgag ggtcagctga ttgaacaccg tttatcgaga
    28921 ggttgtgaaa aagtcgctta gtttcgcgta ttaagaaaaa ttttgtgaaa tggctttcca
    28981 catttttgta taaatttggc ttaataccga gaaactggct tttgaatacc gtttattcgt
    29041 aaaagggatt gtgacaaaag tcttgtcaca atcccttttt atgtttttat tgttctaatt
    29101 gcgctgtctt gcttactgct tcttgcggtt tcacaaattt ttttgagatt ccccaacctg
    29161 tgaaggcact gaccatcgaa aacacaggag aaagcaaact gaaaaatacg aatggcaagt
    29221 aagtgagtgt aggaatacca aatgtacttg ctacgaaact tccggcaatt ccccaaggca
    29281 ctaagtagtt gattactgtt ccaccatctt ctagcgttct acctaataca gttggatcaa
    29341 atcctgcttt ttcataagtt tctttaaatg cattaccagg cagaataaca gataggaatt
    29401 gttcaccaat aaaaatattt actccgatac ttgtcatcaa tgttgtaaaa atcaaatttg
    29461 gtgtgttcgt catctttctt gatactcgtt ccatcacggt tgagatcaag ccaaattcga
    29521 tcaacaatcc acctaatgac aaagttagaa taatcaatga cacagtaggc atcatacttt
    29581 cgattcctcc gcggctaagc agtgaatcga tttgttggtt tccagtattt gaaacaaacc
    29641 ctgattcgat gaccccagcc aacttcgtta aggaagtttg tgggttttga ataaaaatca
    29701 ttcctactgc tacagcgata ttcaacaaaa tcgttaaaat tgccggcacc tttttccatg
    29761 cacacagtaa cattaaaaat aaaggaatca gtgaccagat tgaaatagta aagtttgctt
    29821 ctagaacatc agtcacttca cgaatagatg aaagacttgc ggctttactt gtgtgtccta
    29881 aaatcatgaa taaaaagaaa gaaacaacaa atgccgggac agttgaccac atcatatgct
    29941 tgatgtgttt gaacaaatcg gcatcaacaa tagctgctgc tagatttgtt gattcggata
    30001 atggagacat cttatctcca aaaacggcac ctgaaacaat agctccaaca accagcgctg
    30061 gatttaagcc tagtgttgtc ccaataccga aaaatgcaat cccaatcgtt gacataacag
    30121 taaaggcact ccctacagcg ctacctacga ttgcacacac aacaaataca gaagggacaa
    30181 accactggac actgatcaat ttgaacccaa tgaccattag agttggaatg atccctgctt
    30241 gtatccatac agcaatcaat gaaccgacta aaataaaaat aaagattggg atgattcctg
    30301 gtttgatgcc attgatgatt ccttcgtgca gccaatctgt cgggtatctt tttatcactg
    30361 catacaacat gaccattccg atcgctgcta agatcgtgat attaggtgag acctttaatc
    30421 cgacaacgcc aatcgcaatg cctaaaacaa taaccattaa tacaagtgtt gcttctaaaa
    30481 aacttggtgt tccttttttt cccatgataa tctctccttt tttagttgtt cttatgttct
    30541 aaaaaagtta agatatcgga ttactccgcc gcaattcatc cgcgattcct cctttataaa
    30601 aataaaaaga cctgttttaa actaaggacg aatgactccg cggtaccacc ttaattaaaa
    30661 acagatctgt ttttcacttt caaactttta ttcaaatgta tggactttcc atgtaattcg
    30721 tcaattaggc ttgatcggtt cacagcgacc accgactttc tgaacaaaac acctaaccgc
    30781 tactgcgtga aaagttgact tgtacgaagt ttaccttcca taagatcatt tgtcaatgaa
    30841 aaagaaaata ttttttcatt tttctgaaaa ttatttttat aactttcaca cacaagttta
    30901 ttttttacta gaaagtagcg ccctcttgtt tttttcttgc tactatttct tgatgttctt
    30961 gcttgattct ttctaatagc tttggctctt tcaacagatc aagagctgta ttggcgagta
    31021 gttctgctgc aatcgcaata gaagcaaggc ctttgtcact tttagcagca gctttgaatt
    31081 cttctgtgtg tccggcaata tatgtctcag agattgctac cgtaggctgg attgttggta
    31141 tgacttggct gacatttcct acatcagaag aaccgagact tgttctttgt tccgtttcta
    31201 tttcttcatc tggaacacct gcttcttttg catgctggaa gaataagtca tcaaaagaag
    31261 gagtaacgat gacatcatca actgcatttt ggaacaatcc aaactcaaaa gtcgtgcccg
    31321 tacttatcgc agcacctcgt acgatatttt ccactttttc atatacttta tctaatgttt
    31381 gccgatttcc tgcacgtaaa tagaaacgtc cagcagcgta atctggaatg acgttggcag
    31441 ctactcctcc gtctgtaatg atcccatgga tattgacatc ttttggcaat tgcaagcgta
    31501 gagcgttgat tccattaaac acttggatca atgcttccaa agcatttact cctttttcag
    31561 gtgctgctgc tgcatgggaa gcgacaccgt aaaatttgat gtcgactgga tcattcgcta
    31621 aagattctgt cgttttccca taacgaaaag ctggatggac gcataacgct acatcgacat
    31681 cctcaaaaaa accttctcga acaaagctgc ctttagctga tccgttctcg cccccttctt
    31741 cccctggggt gccatataca cggatttctc caccagtttc atcaatgaat tgcttcacta
    31801 tgctggcggc taatacggag taagtaccaa acaaattatg cccgcatgca tgaccaatac
    31861 ctggtaaagc atcgaattct gccaaaaagg caattgtggg accgggttta ttggaaatat
    31921 aacgtgcatc aaaggctgtt cggtgtccag ctacttcttt ttctacccaa aacctttctt
    31981 tttctaattg tttaatcaac gctgctgagg aataaaactc atagttactt acttcaggat
    32041 gttcgtggat atctagagca agtgcttgat attctggtaa acgatattgg attagatcct
    32101 tcaattggtt tgttgagatt gttgttgtca taaaaaattc ctcctatgct agtcattttt
    32161 cttattttct cccgaatgtt tcccatgcag gcacgcttga tccttttgat gttttctcga
    32221 ttactttgac tgtgtcttct gtttgataag cgtctactat tttttgatac gttttattgt
    32281 tttcatcttc ttttcttgca acgatgatat tcacataagg acgtgcatgg tcatccactg
    32341 gttctaagaa gatggcatca gaagcaggat taaatccagc atctacagct acaccgctat
    32401 tgatcactga aatgtctaca tctgacaaag cacgtgctgt ttgggaagca tctaattcag
    32461 aaatgtctag attttttcta ttctcggtaa tatcagaaac agtgggcgct tgtccttttg
    32521 ccttatccac tttgatcaat ccagcagttt gcaataacag aagagctctg ccgccatttg
    32581 taacatcatt agggattgct acttttgctc cgtctttgat atcagctaca tccttcaaag
    32641 tgttagaata aattcccagc ggtgcattga cagtatttcc gatcgatact aaatcagttc
    32701 cgtgttcttt attgtaattg tctaaaaaga attgatgttg gaaagaattc aaatcaatcg
    32761 atccatctgc taaagccaca tttggttgat tgtaatctgt aaatttcacg tattccaaat
    32821 taatgttctc ctttttcaaa cggtctttca cgttgtccca tacatctgta tcttctccaa
    32881 taatccctag ttttacgata gtttcttttt tgtcagttgt atctgctttt ccttggctac
    32941 accctgccaa ccaaataatt gctacaaatg taatgactac aaaacgaata atttttttca
    33001 ttttttctcc tccactttca atgtgtaatt tttttaatca gttgattact gataaattga
    33061 cttaaaaaaa ctaagattag aatcaataat gtagccacaa tcgttacatc tgtttggaat
    33121 cgattatacc ctctcgatat agcaagattc cctaatccgc ctgcacctac tgctccagcc
    33181 atggtagtca gtccgatcac gttgatgata gtcaacgccg agacccgaat gataccaggc
    33241 aaaccttcta acaaatacac acgtaaaatg ataccgattg gactggttcc catcgcttct
    33301 gccgcttcga tcaccccagg atcaacttct agcagtgcgt tctcgatttg acgagcaaaa
    33361 aacggaacaa ctccaaaaat caatggcact aaagctgctt tcgcaccaat cgtcgttcca
    33421 actaagaaac gagtagtcaa tgctaaaaga gaaagcaaga taatgaaagg gatggaacga
    33481 atgatattga tcagcttatc aagtatttca aatagcagac ggttagctag taatccatta
    33541 gggcgtgtca ctactaacgt cacacctaag atgattccaa tcatcccgcc aatgaatgct
    33601 gtccaaaaag acatatatag tgtttgcttc gtcgcttcga caaattcatc cggaatcaaa
    33661 taaacattgg gcaaatatgt tttaaagaaa tcagtcattt tatcttcctc cttcgatcac
    33721 tttgatacga ctatctaaat cgtttttttg taaaatattt atttttacac cagatttgat
    33781 caaataatcc aatgcttttt tccgctgttt ggtttctcca gctaaagtaa cgatcaaact
    33841 ccctaacggc gtttcttgta ataactctac attgccatat aaaatattcg ctgttacttg
    33901 atatttgctg tacagatgag cgattagagg ttcgttcgtc tgatttccaa tataacttaa
    33961 ttccaccagc catttattgg cgatctgatc ggcaaaagca gaatgggcaa tgatcgtttc
    34021 taatgcttgg tcaagatgag tagctgttcg aataaaatct ttggtcaatt cttcttcagg
    34081 attgctgaag atctggatgc tgctaccttt ttcaacgatt cttccatctt ccataaccgc
    34141 taccttgttg cagatttctt tgacaacttg catctcatga gtgatcagga cgattgttaa
    34201 acctaactgg cgattcaatt tttttaacaa tgcaaggatc tgaagggtcg ttttcggatc
    34261 aagtgcactg gttgcttcat cacataataa taccttaggt tcattcgcca aagctcgtgc
    34321 gattgctact cgttgttttt gtccccccga cagttgttta ggataagcag atgctttttc
    34381 ttccaaaccg acaagttcca gtagttcatt cactttttga cgacgttcct gtttcgattt
    34441 tccagaatac tttaaagaaa aatcaacgtt gtcaaaaata gttcggcttt ccattagatt
    34501 gaaatgctga aagatcatac cgatggcctt tctttctttt cgcaattctt tgggagagag
    34561 ctgactcaga ttcgtctgat tgacaatgac ttctcctgct gttggtttct gcagcaaatt
    34621 catcacacga actaacgtac ttttccctgc acctgagtaa ccgacgattc cataaacatc
    34681 gcctttttca atcgttaaat ctacagaatc tactgcctgt atctcttttt ctttttgttt
    34741 gaaacataca gaaacatctc gcaattcaat cattaaaatt cctccttatc taaaaaaagc
    34801 gtcctcaaga aaatgaaaat gtcattttct tgaggacgct tttttgcgtg ttaccacctc
    34861 atgttcaagc tgtgttcaca cacaactcct cttcaagtac gatcatactc tagcaagata
    34921 tcggtcgcag ccgtcatcgc cttacatcta tgctcgggat gaattgctcg atggccattt
    34981 tccaatagtc gttccttgct tcttctcagc taccgaagct ctctgtcaaa ggttcttcta
    35041 tcgtacttac catttcaatg caaataatat tcaaaaaaaa agtcctttta ctaacataaa
    35101 ctatgtcagt aaaaggacga cgtatcgcgg taccaccttt tttcggaagt ttccttccct
    35161 caagcccaac atctcaaaaa gagagattgg cgttcgtttt aacggtcgaa cagttccgaa
    35221 tttgcctact tatcgacaaa ttattctcca aggccatttt cagcttttct tacttgactc
    35281 ttctcaccag ccagagcctc tctgcaaagt ttccaaactt actttccttt tcaacgaaat
    35341 cgatattttg ttgttgggat taatctacat gaggaaaact gaaaagtcaa gtacgaaatt
    35401 caaattattt tttctatatt tatttttctg tttttttatt taacatttca ataacagttc
    35461 gaagatcttt tccttccgca agaagtttca atttttcctt ttcttcgacg gtataatttc
    35521 ggacaaaact ggaatcaggt atcaaaacaa cgcctaatcc tgctcgtgaa gcagctgttg
    35581 ctcctactaa agaatcttcc actgcaaagg ctgaactagc ttctaatcct gttcgttcca
    35641 aagctgtcag ataaggaatc ggtgaaggct tgttctccga cacttcgtct ccgaatgtaa
    35701 acgtgtccaa gtaatcagct aatttaaagt gatccaagat atctgttgct cttttcgtaa
    35761 cagtggtcgt agcaatgcct agtttcatat gacgttcttt taagtctgcc aatatttctt
    35821 ttgcatatgg ttttaagtgg atggctccgc ttgtcagctg ttgataaaaa tattcttcac
    35881 gcttcgctcg gacttcctgt actttttcag ggctacctgc tgctttcact aaaatatcaa
    35941 aactttgttt ccagtttttt ccgccccaag cgtcaacgat ttcttttggg atatggatat
    36001 caaattgctg aaatccccaa agccagccat cacgatacgt tttttctgtg tcaactaaca
    36061 aaccatctaa atcaaatatt gcacctttca tctatacatt aatcctttcc aaaacaatac
    36121 acatagttta gcacgactgg tccaaaaaga acagaagcaa agaaccagcc ttctcgttta
    36181 taagagttct tggaatcgac ggatatagat tttcttcaca gctgtcacaa gcgccagata
    36241 agcgatgact gtagcaaata accataacca gtaattgcca ggaagtggca tcaagcctaa
    36301 ttcagcacca aaacttgtaa aaggaaggac agaaccgaca ataattccta atgtagtgat
    36361 aacaaacatg gcaaatgttg cgttgctttg gataaatggt actttcggtg tacgcagtgc
    36421 atggagcact aaggtttgtg tccatagtga ttcgacaaac catcctgcat ggaataatgc
    36481 gatgaaaatc actcgctgtg aactatccaa tgtatggaaa cttccgccca ctacagcagg
    36541 acaaataaca aaatacatca acaagtaagt agtaatatca aatatcgaac ttgttggacc
    36601 aaagtaaacc atgaattccc caatactaga tgcattccat ttttttggct cgtccaaata
    36661 ttctttatcc atccgatccc atggcaagga aatgcaggaa acatcataga tcagattcaa
    36721 aaacaaaagt tgtaaaggca gcattggtaa aaatggcagg aacgtacttg caatcagcac
    36781 ggaaaacata ttgccaaaat tagaacttgc tgtagccttg atatatttca tgatattccc
    36841 aaaagtttct cgtccagaaa gaaggcctct ttccaaaatc gtcaaatctt tttctaaaag
    36901 gatcacgtct gcagattctt tcgcaatatc cacagctgta tctacagaaa taccgatatc
    36961 agccactttc attgcaggag cgtcattgat accatctcct aaaaatccga cagtatgacc
    37021 atttttacgt aaagctgtcg tcaaacgtgc tttttgctga ggattcaatt ttacgaatac
    37081 ttcatggtct tcagctactt gtgctaattc atgatcactc atttccgaga ttttctcacc
    37141 ggtaaccaat tcattgatct ctagacctac ttgacggcac actgaacgtg taactaattc
    37201 gttgtcccca gtcagaactt ttacagctac accatgttct ttcagagctt tcaacgcttg
    37261 tttcgttgtt tcttttggtg gatcaaggaa agcaagataa ccaatcaaaa ccatgtctga
    37321 ctcatctttg actgaaaatt ctccaacgac gcttggattg gtcttttgtg cgacaccaat
    37381 cactcgtaag ccatcttcat taagtgactg tacttttgca agtacggctt cttttcgttc
    37441 ttttgttaat ggtgtaacag taccgccaag atcaataaat ttagaaatgt tcagcatttc
    37501 ttcaatcgcg ccttttgtga tcatttgtgt tttaccagct ggatcagcaa ccacaacact
    37561 catacgacga cgttcaaaat caaaaggaat ttcatccact ttttgataat tgatcttatc
    37621 tgtatctaaa gtttctgttg cttcatcaat gatagccaca tctaacaaat ttttcagccc
    37681 tgtttgatag tagctattta gatacgcatg gcgcaaaaca cgatcatctt catttccttc
    37741 acaatccata tgatattcaa gaatgatttt atcttgtgtc aatgtccccg ttttatccgt
    37801 acataacaca tcgattgcac caaagttctg gatggcattc aaatttttga tcactgttcc
    37861 ttttttggcc atcacacttg ctcctttgac caaattcgtc gtaacgatca ttggaagcat
    37921 ttcaggtgtt agaccaactg ctacagatag tccaaaaaga aaagcttcta gccaatcccc
    37981 ttttgtaagc ccattgatga cgataactgc tggagccatc aatgacataa atttgatcag
    38041 tagcatggat gttttatgga tgcctaattc aaagcttgat tgaattggtt tttcagatag
    38101 cgtttctgct acatgaccaa atagtgtttc atttccaaca gaaacaatga ttccttccgc
    38161 acttccactg atgacattac ttcccataaa gacaagattt tcgtaacttg tttcgctttg
    38221 ctgatcgtct tcacatactt tcgcatgctt ttctactgga tagctttccc ctgtcaatgc
    38281 tgcttgggaa atgaataaat cctttgcgga caataaacgc atatccgctg gtatcatatc
    38341 ccctgctgaa agccgaacaa gatctccaca gacgatttct tccataggaa tctctgtgta
    38401 ctttccatta cgttttacag ctgccgttac tttcacaagt gatttcagtt tttcagccgc
    38461 ttgattggaa cgtacggact gaatcaacgt cattgtcccg ctgacgataa ccatagtgaa
    38521 gataatggct acaccgaaaa gatctttatc tccaggagct gctaatacat attccgtgat
    38581 gaatgaaata attcctaatg cgatcaacac taatgtaaat ggcgtgatgt atgctttcaa
    38641 tatttctaca atgaatggtg tttttttgcc atatgaaatc gcattttcac cgtaattttc
    38701 tcttgaacgt tcgatttgtt cttctgtcaa tccatttgct gatgcatgga atagttcaaa
    38761 taattcttgt ttcgtttttc cagcaaaagc tgcatagatt ttttgtgtat ctttcttttt
    38821 ttccatctca atctctcctt tgtgtccaaa agacagctgt tctcgctgct tttggtcttt
    38881 agctattcat cggatcgata gagaaaaaac tgaaaggggc tgagaacaga tagataatag
    38941 ccctggcctt tcctctagat ccttgttttt cgaatgactg tcatccattt gttctcatct
    39001 cctttactaa gtaattttaa ttcatgactt ccaatcattt ttcgaatagt tatttttcca
    39061 attagaccac ctccttttta tttttcaccg atggggattt gcggcaacaa aaaaacctct
    39121 cgccaaatac tcagcgaaag gtttgaaaaa gcatattaaa aaaagcttct cagatctcat
    39181 cgttgagttt tgacactgta caacgtaaaa agcagatacc tgcttttagc taccttatga
    39241 aaagccttat ctcgacaatt cctgttctac ccattggcgt ctctcgacgt ttttgggcag
    39301 tagcctatgt ttgtatagga gcctcaccta acaaatctta ttcggttttc cttcgttaat
    39361 ataacaacta aaaaagagga tgtcaaatca attttccatg tttttcatac tttttttaaa
    39421 atacacacgt tatattcctg tctcacgcgg ttttatttgt ctcattttcc acctcccgct
    39481 ataatagaaa tatagtagga aacgaggtgc tgttatggaa agaacacttg ttattatcaa
    39541 gccagacggc gtgagacgcc atttagtcgg aagcatcatc caacgttttg aagcaaaggg
    39601 actggccatt gctgaaatga agtttgaaac tatgactcct gaattagcaa aagaacatta
    39661 tcagcacttg actgagcgca gtttttttga tgaactgatc gattacatga cttccggtcc
    39721 tgtcgtttat cttgtgctgg tcggcgagga agtgatcgat atcgtccgta agatggttgg
    39781 agcaacaaaa gctgccgatg ctgttccagg tacgatccga ggagactacg cgctgccggg
    39841 gactgaaaac atcattcacg cttctgattc ccgtgatgct gcggtaaaag agatcgcccg
    39901 ctttttcccg ccattcgata taaacaaacc gtctgaaaaa gcggcgaagt gatacaaatt
    39961 ctaaactgct ggtctagttg ctgggttcag caatggccag cagttttttc tctaaggacc
    40021 tccgttgatt ctttcctaac atatattcga gcactgtgaa gaattcttgg tagtctttcg
    40081 tcttttcttc ccctaaagca gcagaaatca atctttgata ataatctgcc atcaatgttt
    40141 catagatcac tgtacttctt tctttatgtg ctaagctccg tttcatatct tcattcacac
    40201 tacgtgcctt tttcaaccga ccattttcta tataaaacga agtcaggttc attaaaaaag
    40261 taaagagata ctgctcatga ttgttcaact gtgctaatcg tcgcattctt tttttgattt
    40321 tcaaataatt gttctcaatg aaccgatcac tgaatatcca aggaaaatca gctagaatag
    40381 atagttcaaa ttgaccatag tctggtatct gcatcaagta ttcttcaatg attcggcatt
    40441 cataacgtaa aaattcaaat tctgtctcag atacttctgt tttttcgacc atgtgaagat
    40501 tcaaaaccaa ataatacaga tcttctgttt tcttaaaatg cctacggtta ttgctgatct
    40561 ctttataagg atcaaagctg tcttcttgta cccgatctag cattcgtctt tttttcttta
    40621 actttattgg ttcatccagc tggtttcgca tccattcata ttctgcaagt gtcatgttca
    40681 ttcgctctaa aaatgatctc aatgttacaa atgacatttt tttcactgaa tttgtctcaa
    40741 tcccgactag agaagtctta aaacagattc cttccgctaa ttgggactgt gtcaacttct
    40801 tgttgactcg aagtttcttg atcaattcac catcatacat aatagttctc ctttttaatt
    40861 taaatttttc cacattcaat aaatagatac gttaaaaagg aaaaatacac gaaaaaaata
    40921 aaaaaatgtt tttttcataa aaaataccaa ccttcaagca ttagaatgtt gtctaactac
    40981 tgaaggttgg tttgattgtt tattgattat ttttttgctg ttcaacgtct gttcttgtta
    41041 ctggatataa gttggttgtt gttttattac cattttttga aaagacactt gtcgaatgtt
    41101 cgcctttttg cttttcgatt gcttcattac gttccgcttc atttttatag tcatattttt
    41161 tcggatcaat cgtctcaaat ccgtttggca cataaaaccg caagagattt tcctgattca
    41221 gcttatctga aagacttaac gcagtcttga ctttttcctg atctttggag atttcctcat
    41281 cgattcctgc tgctttcgta tcgatgattt ctcctgtctg attattgtaa gcttttcctc
    41341 ctaggacggt gtatttgggt gtcacaaagt tcccatttcg aaaagcaact acttgatcat
    41401 gctgagaaga taataagtct gtaccaaaat gaagataatc tttatcatcg attcccaata
    41461 gatgtagtaa tgtcggcaaa acatcgattt ctccaccgta ttcatgattg actgtccctt
    41521 tagtataacc aggcatatgg atcatcaatg gcactcgttg catctgtgcg ttgtcaaatt
    41581 catcccatgt atctggatct ttaccaagag cgcttgctaa atctttatta tctgtattcg
    41641 agatcccaaa atgatccccg taaatcacaa agattgaatt ttggtaaata ccagattttt
    41701 tcaaatacgt aaaaaattgt tccaatgatt gatccagata atgtgccgtt cgaacataat
    41761 tatctactgt gctattgcca gtattcaaag atggaaagtc gaaattcttg tcatccgtat
    41821 aataaggcgt gtggttggtt acggaaagaa atttcgtgta aaacggctgt tgtagatgct
    41881 caagatattg ggcagattct ctgaataaat ctttatctaa tatcccataa cctaacgttt
    41941 cgtcggattc atcaaaataa gatcgatcaa agaaattgtc ataacctaag tttttatata
    42001 catgatctct attccaaaaa ctagctacat tcccatggaa aacagcgctt gtatagcctt
    42061 gcttttgtcc aaggatcgct ggtgcggctt gaaagacatt gtctgaccca agttctgtga
    42121 ataatgagcc ttgaggtaaa ccaaatgtgc cagtttccaa catattttca gcatcacttg
    42181 tctttccctg tcctacttca tgaaagaaat tgtcaaagct gatcgttgct tgattctgga
    42241 aaatactatt taaaaacggt gtgacttctt gtccgtctac cttcatatta atcaaaaact
    42301 gctggaaact ttctaaatgg agaacgatca catttttccc tttagcgatc ccaaatgttt
    42361 cgggatttgg ctctgcataa tgtttctttg tataatctaa aacttcatca atcccattac
    42421 tgcttgcatg cgcgcgaaca ctgctagtca ttcctgtttt gattccgtca taaatagtat
    42481 acgcatcgat tcctagatat ttgacaatat atgaacggtc aaatgttcgc tgcagcaatt
    42541 gtggacggtt ggcttcgctc agcgctagat ttcctgaaaa aataagccct gaaagacagg
    42601 taacagcgat agccatcggt ttgccgacag ggttgctttt gataggtatt ttcttgacct
    42661 tcaaccaaac cagtagccca atgaacacag cgatatccag ccagtagatg acatcatggg
    42721 gcttcatcaa agaaaaggag cttcctgaca atccttgcga cactttcgaa aagcctaata
    42781 cagatttgac tgtaatgaaa tctgtgaatt cccgataaaa aataatattc aagtacaaca
    42841 aaagcgtatt taaaatatca atgatcaaaa gaatagctaa cgctggcttt ggctttttaa
    42901 tatacaaaga aaggccaaaa aacaataagg tagtcgcaat tgggttgatc cacaagataa
    42961 agtactggat cgtaccttcc acacctaatg aaaaatctgc atagtaggca atgatcgtct
    43021 tcagccagaa aaagaaaaca gctgtcaaaa cgattgctaa acgattttgg cacaattttt
    43081 taagttgtgt cacgtatatc acttctctct attcacattt tttcttattt tgctcctgaa
    43141 aaatgaaaat ttcctaaaaa agttgttatc ttaagatttt tttattcttt ctaaagatta
    43201 ttccgtccac ttttcacagc ataaaaccgc tgcgatgtaa gcgattaaac taatgaagag
    43261 ataaaagtct tgtggtgacc gcttcactcc acggtattca aaagccagtt tctcggtatt
    43321 cgtacaaaaa aagaggctgt gacaaaattc ttgtcgcagc tccttatttc ttgatgcagg
    43381 acagcttttt cacaacttct tccttaaacg gtgttctcct gccaactcct cggccacaag
    43441 tcacaatatt tataaaacat gagaagctgt tttttgaaat tgttccttgt ggctcagagt
    43501 taaacggcag gctcaagacc tcttgataca cggtgttcaa tcagctgacc ctcggtatta
    43561 gattatgtca ggacgctaat tttacaacct cttggtaaac ggtgttcaaa agcttgcatc
    43621 tgcggtaata agcccaacca aacgaaaaat ataaggagct attttttgaa attgctcctt
    43681 gtggctcaga gttaaacggc aggctcaaga cctcttgata cacggtgttc aatcagctga
    43741 ccttcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttgccg tttgtttctc
    43801 taatactcag gtcaaaacgc tgatttcaca acctctgttt ttcattgtcc ataggattga
    43861 tattgtcctt gtgatgactc attcgtaata attgaggctt gattttcttc acttcccgtt
    43921 atcgtttgtc cactgccaga atctgaagga cttgttcctg tcgactcgtt tttttgagaa
    43981 gagacagttg ttttcttgac ttgttcattt gtctttttcc attccgctaa acgatcggcg
    44041 accacttttt tgtctacttt ttcagaataa gcggtgatca cttttccatt tttcaaacgg
    44101 gctactttgt cgccatcatg agtcaaaagc aaaacggtca aatgcttgtc tttaaattga
    44161 tattgtctga gagaaatcgc actaagtacg tcatcgatat taataaattg attttcttct
    44221 tttccttttg gcaaataaat gtccaattcg cctttttcgt aattggcagt tattttatac
    44281 tccgcccacc aataagttga gaattctttt ttgaaattat ctactgattc tttgcttgct
    44341 acgatttttt ctgtttcttt ctgagcaaga tctgctgtca tttttcctga aaatgctgcg
    44401 gataaaataa gcagtaagaa aaaaccgaat aaccaaaaga gaagaaacgg tctttttttc
    44461 ctcatgcttt cctccactcc tatttaattt tgactattct attatataca aaaaatgaaa
    44521 tagaaagaca aaaaagttga aaaagatcgt aaataattga acttcttttt cacaaaatgg
    44581 taagaatgga gaatttcacg catttgctct caactattct ttcttaagcc tcaaatccgt
    44641 ttttatctga tacgtctttg aaccaatgac ccgatttttt gattgttctt tttccatctt
    44701 gtgctaagtc tactgaaata aagccatatc gattcttata tgcattggtc cacgaccagt
    44761 tatccataca tgtccacata tgatagccta aacaattggc accttcttga atggcttgat
    44821 ggacatattt cagatgagat tggacaaatt cgatccgata atcatcgtgg atcatgccat
    44881 cttctttgat aaatcgtgct tcgttttcta cacccatacc gttttcagaa ataaagcagt
    44941 cgatatttcc ataatgatct cgtacattga tcaaaatatc atagattcct tgttcataga
    45001 tctcccatcc gcgataagga ttcattttac gaccaggcat ttcataattg tcgaaataat
    45061 cctcaggcat tggaccgttt ttagtatcga ttggtgtttc ttttgctttt acccggcgtg
    45121 gctggtaata attgatacct aaaagatcta ctgtattttc tttgataacc gtcaaatcct
    45181 ctgcatggat cacaggcatg agatcaagtt cttttactat ctcaatcaat tcacttggaa
    45241 actcgccttt tacagctgga tcaaggaatg aacgattgaa gaacgcatct gctatttgcg
    45301 ctgctttaac atcttcaggg tttgattcgt ctcttggata gcttggtgtc agattcaaga
    45361 tgataccaat aacaccacct tgctccattt catggtaaga tttgatggct aacgctgaag
    45421 ccaacgcttc atgatagcca acttggatcg cttttttcat atctgactct tctggataat
    45481 gccatcgata taaataacct gcttcgactg gaacgattgg ttcattatga gtaaaccatt
    45541 tttttacttt cgaaccaaac aattcaaaac aacttttggc atatttttca taggcttcaa
    45601 ccgtttctcg gttcaaccat ccgccttttt cttgcagata catcggcata tcgaaatgat
    45661 acagattcac aaagggttcg ataccgtttt caatcaactc atcaaaataa cgatgataga
    45721 aacgaacggc ttcttcgttg attgttccag attcatcagg tatcaaacgg ctccattgga
    45781 tagatgttcg aaatgaatta tgcccagtct gtttcatcaa ctgtacatcc tcttgatact
    45841 tcgtataaac ttgcgaggtt ttttcaggtc ctacttgatc aaagaacttt tgcggttctt
    45901 ttttgtacca ataatcaaaa atgttctctc cttttccatc gcctgaaatc cttccttctg
    45961 tttgtgggcc acttgctgca gaaccccacc aaaaatttgc tggaaattga tattttttca
    46021 tcaaagcacc tctttttctt ttttagttcg ttttagttga ttccatgtat gatattttcc
    46081 atcacaatca ctttttcatt ttcttcctct tcttttttga ttcgttttaa cagctgttct
    46141 gctgctcgtt ccccgatttt atctgttggt ggcgtgatcg tgattttcgg aaggatactc
    46201 agatcgtttt tatctaaata atcatagtga ctgaacgcaa tatccttccc gattctgaca
    46261 ccttcttgat tgaaacattc gatggcacca atcgacatat tgtaattgct gacaaaaata
    46321 gcagttgccc ctttttctaa taatgctttt gccccgtaat agccgctttt tctagaatag
    46381 tctcctgtaa agacctctaa ttggatcgat ggatcatttt ggaccgcttc tttggctcct
    46441 gctaaacgtt ctcttgctac gtagtctgtt tgagtggcag caataaatcc gattttttta
    46501 tgtccttgtt caccatgatg agcgatcact tttgctacac tttctcgatc attcactaat
    46561 attgtatcaa tacctgctgg tccttctgga caattcaatg cgataactgg aatactcatt
    46621 tttgtgagca tttcaaatcc agaccactct tcttcggata agaagacaat cagcccatcg
    46681 atcacatgct tcatcaggta ttcaattttt tgttcaattt tttctggctg atcggcaaac
    46741 ccgcttaata ataagctata cccattttct tcagctactc tttcgatccc tgagacaact
    46801 tccgacccaa aacggctgga aatcccgttt atcaaaagtc caatagagaa gcttcgatta
    46861 ttcttcaacc ctttagcaat gatgttttct ttatagtcca acgctgaaat cgcttctgct
    46921 atttttttca tgttcttttc cttcagttgc tgaccattca agtaacggga aactgttccc
    46981 actgaaacac cagccatctt agccacatca cggatcgatg ccatacattc tcactcctgc
    47041 cagttacatt tgatttctct ttcttgttgt tcgattatga tgaccgcatt ctttgcatag
    47101 tgttgcttgt catcaatatc catataacta aagtcttcag tagagcaact tcctctcact
    47161 attaattata cttaaacaag tatttgaacc ggttcatttt taatatagca tttttttaaa
    47221 agattgcaac acttttttaa aacgctttct gaaagtggcg gattttttgc ctttttaaaa
    47281 ttttattcta aaatttactc tatttgtggg tgttttgtgc gtttttctct tccatccgtt
    47341 tgaagtttta cattccaatg gtataatttc tttatcctat caaatggggg aagtattttg
    47401 aagaaattaa tcgtactatt tattttgtct gcgggacttt tgacattgac tggctgtgat
    47461 ttgtttggcg gctctgacca atcctcggac gaaacaaccg attcttctgt cttaaaagcg
    47521 gaaaaagcag ataccaccaa cagtgtatat acgctaaaag tacgtaaaga tatggattta
    47581 tccgcgggac agttgacatt tacagataaa aaaatggaat ggacacgcac gtatcagaca
    47641 caaggaactg attcttctga atcatctgaa gaaaaaacag tgctgacaga tatcaagatc
    47701 actaccaaag atgacgaata tattatttca ggaaaagaaa ataaaaaaga aacaacagtc
    47761 atatttacaa aaatcggtaa ttaccgtttg aaggatgagg atggcaacat ctacagtcta
    47821 tagtgccgtc tgttggatct tcttttgttt ctccccttct tttctgctat agtaaagaag
    47881 caagatgatt gtaaaggaga cgaaatggat gatcgatagt cctgattatt gtacatgtaa
    47941 taacaacaaa aatatccata cagaatttaa tgaccaagga agcttgagca tatgcgacaa
    48001 ctgtcacaaa ctagtcaaag attcatttaa aacatttgaa tcttatcctg ttcaaaatta
    48061 tgtaaaaagt agaagaagat aaacaaatcc aagcaaaact cactatggct gatttttatg
    48121 aacttcaaac tcagcttctt cctttgatca agcttcatca gtaaaacaaa cagtggatca
    48181 tttcagttta caaatcaaag agtaaagatt tatttatttc aaatatttga atataaaata
    48241 accaaacgat agaaaggctt attctacatc acgtagtaat ttttctttcc cgtcgtttgg
    48301 ttttttattt ttagcctaat tcttgacctt ttgcatgctg gttatccgta aaatattttc
    48361 cagcaggctt tccttccaat tcagctaatt tttcttggat ctgacggatc accttttttg
    48421 catcttctgg attttctaca aaatcgaatt tatccccatc cacttgtatt ttaggacata
    48481 catcaaaatc ttcataccat tgattatagc gggtgttcaa cgtttcataa taggtatata
    48541 acgtctcatc gctttctagc tgttcatagt cacgcccacg ttttttgata cgttctagca
    48601 tcgtgtccaa agaaactttg atatggacca tcaaatcagg ccgttttttt ggagcaacat
    48661 catctaactc gtttagcatc gtatctaata agctatcgta ctgctgtact tcgatatccg
    48721 ttacccgacc taaatcagcg tttagatgga acaacaggct atcttcatag atggatcggt
    48781 ctagcacatt gtcatcgttc actaaagcat ttttcattgc taaaaatcgc ttgttcaaaa
    48841 agaagatttg caataaaaaa gcgtactgct caggatttga ataaaagagc ggcaatactt
    48901 cgttatcttc gacattttca taaaatggtt tagaatcaat ctcttttgca agcatatccg
    48961 tcaaactgct cttacctgca ccaatcgttc ctgaaatcaa aatcactgac atacaaccct
    49021 tcctcttcta taagtactcg cctacgtaag tttcaacagg caatgtttca aaatcatatt
    49081 cacgtacagc ttggtctgat tttaaatacg tatggagact ttgtgtaatc atcaagttcg
    49141 taggtaccgt ctcttcttgt acaatgatca tcggtttttt caacatagtt gccacaccta
    49201 attcgtaagc cgttcctgga tcgattgttt gatgctcaaa gtcaaggata gcaacaacaa
    49261 attctgcatt tgtcagttct tccatatctt tttcgtatac ttcctgcgcc catccagcgc
    49321 tgaatagttc ataattgctt tcttggtgat gacgtggaga gtagaaactt gtgactgttt
    49381 tgttttcctc caacgctttt tcgatacgac ttactcgatc gatttgctcc tctgaaaaaa
    49441 atggtcctgc taaatatatt tgactcatta ttttgcctcc ttcaataata actgattttt
    49501 aatagatact gctcgtaatt cattgaccag ttccaatgga ttgttggaaa agattacttg
    49561 atccaaccat tttttatcca taaaatcctc ttttgcaaaa gtttctaata attgttgcaa
    49621 gccgctgtaa taaccgccaa tattcaatat tccgattggc ttgtcgggcg tcagaccgat
    49681 tgctatccaa gaaagaagtt gtgagaattc ctccaatgta cctagacctc cagggaaaac
    49741 gaggacagca tctccctttt caaataataa tacctttctt tcatcgatcg tttctgtagg
    49801 aataaatgta gtaacatctt ttccaggcaa ttcagtttca aaaagcccat tcggatagat
    49861 acctgtgact tttccgttat tttccagtac tgttttacta atcactccca taatcccgga
    49921 gtatgaacca ccgtaaacca gttcgatttc ttcttgtgcc atgtattttc ctaacgtttg
    49981 cgcaataaat ttgtaacttt ctttattacc aaaacgggaa ccgcaaaaaa cagtaagttt
    50041 cataaaaaaa cactccctaa ctcaaaagtc tatcttcatt ctacacgtgt ttttttaaaa
    50101 taaaagacta gattttgtag ttttttagtg gtatatagga tagacgccac tatatatagt
    50161 atttagaaaa aaatcatttt tcagccattt tatagataaa acaaaaataa ataacgtgtt
    50221 ttcatcgata tgaaatgact tacttataga agatactggg atttcgtcct atatgccaat
    50281 aacgtgtatt attttaaaca acaaatactt gtttctttta ttaacgaaaa aatcattttg
    50341 ttctcgtgtt atgtttcact gataaatgat gtttctctaa ataaaaaagc cgacgaacta
    50401 aacgatcaaa atttgatcat ttttgttcat cagcttctcc taaaaatctt tttgttaacg
    50461 ataaatagct ggatttgtaa aaatatcact tgaatcgtca ctgctggaag aaaattcagt
    50521 gataaccgca cctgttgcgc ctgcctggaa ctagtgtttg atattgggct cgaacgtgta
    50581 ctgatctcta gcatgaagaa taatttcttt ttttactgta taataacttt ctttcctttt
    50641 tggaatagta caggaaagtt tttttgtttc ctttccttcg acataaggta taccagaccc
    50701 tttcgacaac gaaaggttct catttttcct tcatcgtcat ttcaaggagg gtgccgatgc
    50761 tctggacagt ttcgatcgct cctaaaccaa aatctgtata ttcgattctt tctaactctg
    50821 attctttcat accattcctg tctcaaggta catctattgt acttttttct tgtaatccat
    50881 tctgcctctc ctaactaatt gtcaaatcat cccaagatat gtcttcttcc tctttttctt
    50941 cttggatgat ttctgcattc ggatctactg gttttttcaa taataaaatg gttattcctg
    51001 tgacaaaaga accagctaac aatgcaccaa tgaatgccca tggtctggtc atcgtagcga
    51061 ctgcaaacat tcccgatgcc ggaatacggc tatctgcgcc ccaaaacatg cttaatccgc
    51121 cacctactgt cccaccgact cccgttgcta tgacattgcg tgccaagtcg ttcaatacga
    51181 tagggaaaca tccctctgtg atctgacaga tccccatagg aaaagcggtc ttcaatgtat
    51241 cgatttcttg acgattgagt atatttttac gaatgatttt ccctaagaaa taagcgatcg
    51301 tcaagccgaa tggggttacc atcgaagcta aaatcaatac agtgattggt tcatagattc
    51361 cttctgaaaa tcgtgccaca cccccttatc cagaataaat ggtaatccag aaacagccgt
    51421 gtatctgcgg taataagcct aaccaaacga aaaatatgag aagctatttt tgtttggctg
    51481 ttcttattac ttgatgcagg gcagcttttt cacaacctct ttttttattt atgtttcata
    51541 atcaatatgg taaggatgca gctccgtatg gtagaccgcc tccgaatatt caatcatttc
    51601 cccaatttgc gtagacgatt tccgtatccg tttgatgccg atctgtgcgt ctgttttcag
    51661 aattccttgt tcttcttctg ttaaaaatac tgccgagaaa tcatcagaaa atgagtcaat
    51721 agtatatcct tttttcgtca gcaaacgata caaagattct ttgccaaatt cttctctgct
    51781 gaattgcatc atttcttgtg gaagataatg aacaaaataa atataaggct gattgttcaa
    51841 ataatataag cgcttaaaac aaacagcttg ctcaccgaaa aaggaaaaca aaggatcttc
    51901 cttctttata tggaccaatt gcaaatccag cgtttctttc cggacttcat atcgggagtt
    51961 ctccaagatt tgcgtaaagc ttgccgcttt ggataatcga ttgtaaggac gattgcttag
    52021 aaccgtcgtc ccacgaccac ttttctttgc gacatattca tctgatgcca gcagttcgat
    52081 tgcctttcgg acagtgattt tgctgacttg aaacagttct tctaattcac tttctgtcgg
    52141 caacatcgaa ccgactggat attttcctga gaaaatatct tctttaattt tatctgcgac
    52201 ttccaagtat agtacacgtg tccttttcat aacgtcaccc tttgctttac tattactaaa
    52261 agaatagcac ataaaaaata aaaaaagaac acaaatcaca tgtgagaagt gtgttctttt
    52321 tcattcagct gctttaatta caagtttacg ccatttgagg caattacttc tttataccaa
    52381 tcaaaagatt ttttctttga tcgattcagt gtcccatttc cttcatcgtc tagatccaca
    52441 tagataaagc cgtaacgttt actcatttct cctgttgatg cactgacaag atcgatacat
    52501 ccccaaggcg tatagcccat caaatctaca ccgtctgcta ctgcattagc catcgcttcg
    52561 atatgacgac gtaaataatc gatccgataa tcatcaataa tcgtgccatc ctcttccata
    52621 tgatcaatgg ctccaagacc gttttcaaca atgaataatg gtttctggta acgattgtat
    52681 aattcattca atgcgattct cagcccttct ggatcgatct gccagcccca ttcacttgcc
    52741 tcaagaaacg gattttttac tcctcccagc aagttccctt ctgaagttgc tgcttgtgga
    52801 tcagtctcat tgattgccgt agacatataa taactaaatc cgatgtagtc cactggatat
    52861 tcttttagca actggaagtc tttttccgtt tgctctaatt tttccggatc gactccatat
    52921 tttttatggg tacgcgctgt gtatgcaggg tattctccac gtacttggac atccgtgcag
    52981 aaaaaattga attcttggtt ttggatcatt gttgctgcct gattgactgg attggcatcg
    53041 atgctgtaag ttgttgcata gataatcatg cagccgattt ggatatccga acgcaattcg
    53101 tggccgattt ttacggcttt gctacttgca acgaattgat tatgccatgc ttggaaaata
    53161 ttttggtact ctcctgctcc attgcttttt actaatcctt gacttaaagc tggaaagtga
    53221 aaggccgaat tgatttcatt gaaagtcatc caatatttga ctttactgct gtatctctct
    53281 aatactgtct gagcaaatcg ttcatagaag tcgatcagtt ttcgattttt ccagccaccg
    53341 tattcttttg ccaaatgaag aggcatttcg taatgagaaa tcgtgatcac aggttcaata
    53401 tcatatttta agcactcatc aatcagctga tcataaaatt ctaatcccgc ttcgttagga
    53461 gtaccttcgt ctccatttgg aaaaattcgt gtccaggcaa tcgaaaaacg ataacatttg
    53521 aatcccattt ccgcaaacaa ggcaagatct tctttgaaac gatcgtaatg atcgattcct
    53581 cgatgattcg ggtaaatata tttttcttga tcaatcgtcc agtcaaattc ttcactgccg
    53641 ataatcgcaa agcgctgctt acctccaggc atagcatctg caacggatag tccttttccg
    53701 tctcgatcat aagccccttc taattgattt ggcagcagtt gctccacccc ataaaaattt
    53761 ttctgaaaaa cgtgattgca tcctgatttc ctccttattt tacaattgcc agaacatctt
    53821 cgtttgcaat aatctctttt tggtctaatt caagtatatc ggcaaatgca tcagtattcg
    53881 taatgacaac tggagtgatt acgctatagc cagctgctct aattgcttcg atgtcaaaac
    53941 gaatcaataa atctcctgct ttgacgagat ccccttgttt gactaagatc tcaaagccat
    54001 tcccatttaa ttctactgta tccatcccga tatgaatcaa aatctccgct ccgtcagtcg
    54061 ttgtgatccc tacagcatgt ccagttggaa aaatagtcgt gatctctccg tctgcaggtg
    54121 caactagttc tcctacttct ggatcaatag cgataccttt tcccatcacg ccagaagaaa
    54181 aaacttggtc tggtacttca ctcaatggca gtactttacc cgttaaagga ctttttaagg
    54241 tatgccgtgc tgaagtaatc ggttttcctg cattcgcatg tttgttttct aattgattgt
    54301 cttgtgtttg ttcgtcaaat cctaaaatca atgtacctac gaatgcaaga acaaaagcaa
    54361 ttcctgtagc tattactgct actgtcacat ttgactctac accatccacc gtattaatga
    54421 aagtcggaat cgtcaataag ctgaccaaac tgctggagaa agcttttact ccggaaaatc
    54481 cgataatagc tccaccaata cctccagaaa tacaagcggc aatgaatggt ttcttcaaag
    54541 gtaatgtcac accatacaca gttggttcag tgattccaaa taacgaagtc actgtagaag
    54601 aaatccctaa tgctcgaagt ttcgtatctt ttgttcgcaa tgctacagct aaagcagccc
    54661 caccttgtgc caagatggct ggaagaagca ttggcatcaa tacatcaaac ccatattgtt
    54721 cgatattcaa aaacatgatc ggtacgaatc cccaatgcat gccgaacatc acgaatactt
    54781 gccataaact tcccatgatt agacctgcta cgattggact aaatccataa atcgagttga
    54841 agagaccgcc taaagcatta ccggcaaccg tcccgattgg tcctaaagca agaaatgtta
    54901 atggaaccat cactactaaa acgacaagcg gaactaaaat catttgcaga aattgcggaa
    54961 tcactttttt tacgaatggc tctaatttac tttgaaccca tacggctaga atgattggta
    55021 tgacagaaga agtataacca gatggtgaaa gaatcactgg gatcccaaag aaactgatag
    55081 cttctcccgc accagcaact gctgtgatag ctggatacac aagtgccata gcaattgcta
    55141 ctgctaaaaa ctggttggct ttgaactttt tagctgctgt aaaagcaagc atgacaggta
    55201 agaaggtaaa gacaccatct gctgcagcaa acaatatttg ataagtaccg ctatcagctg
    55261 tcagccagcc catcaccgtt gccaatgata agaatccttt caataccccg gcggctgcca
    55321 tcgcccctag aaaaggtgta aatatactag agataatatc gatcagacga ttcaaaatat
    55381 ttttggactc ttcttttcca tcctccgaaa ctgcaagacc ttcctcatcc acgatcgatt
    55441 gataaacatc tgctacatta ctgccaatca ctacttgata ctgaccgcca ctttgtacga
    55501 cttggatcac gtccgggtct tcttttaact tttgcgtatc tgctaaagat tcatctttta
    55561 atctgaatcg caaccgcgta gcacaatgta ctaaactttt gacattcttt tgtcctccta
    55621 ccgcttccca tacacgacga ccaactgctt gattatccat tgttttttcc ttctttcttt
    55681 tttatttaaa aaataaaaac ccagattgaa aaaaacagtc acaaacattc gtgaacctat
    55741 tttttcaatc caggttttgc ctgccataca gtaacaatcc ttatgattgg atgctttttt
    55801 tctcacttac tactctttga atatgaatcg ttaaataaat ctgctctgcg atccccattt
    55861 cctgtttttg cgtttgtcga agatactcat tgatccgttc gacacaatga aacgcttttg
    55921 gatactttcc ttgaactaat gcgtagagaa attcatcttg ctcgtcagat ccttcccctt
    55981 tgagataacg ctgggcaaaa aactgcagat gagtaatgat acgctgataa ttcaaagatt
    56041 cttcatcgaa cacgatgccg aagaatttac tgatgatact cagtatatct cttaccattg
    56101 tggtagcggt catcgtcact tgcatgtctt cgtttccttg ttggctattc acaaaatgaa
    56161 aggcaataaa accggcttca tcctcagaaa gttccacacc cgtcttttgt ttcacgaaca
    56221 aaagagcctc tttcgctgct tgatattctt tcggataaaa tttttttgtt tcaaataata
    56281 atggattagg cagttggata ttttcttttg ctcgtgtgat cgcataatga agatgatcgg
    56341 tcaatgtcag ataaatatta ggcgaaaatt caacttttag tttatcttca gccagttcga
    56401 ctacttgttt gacccattcg atctcctctg ccggaatatc agccatcagc tgctggaatt
    56461 ggtttttagc aaaagaatct cttaaaacaa attctttttg gaccgcttct ggcgaaatct
    56521 cgtccccaat ctttttttga aaagctagtc cttttcccat acaaacgact tcttccccga
    56581 atccattttt cgaaatgacg acattgttat tcaaaatttt atcaattttc attttgcttc
    56641 gccctctcaa gaaaaataaa aaaacctaga attcgcctac tgtttcttag ctgaattctg
    56701 gttttgcctg ccgtacagta acaatccatt tcttattcaa aatcatcata ccatcgaatt
    56761 tttatttatg caagcctttt catataaaaa ataaaaaaac tggaacacaa gtctatctag
    56821 aaaacaacca aatcagagat gcacctcttt gcgtttctct ttttttcagc aaattggttt
    56881 ttccttatcg attcatatcc tttggctgtt tttaatctgt aacgacttat gtcccagtat
    56941 cctaaaatgt ttttgtctct agattgaaag cagatctcat atcatctgtc gcatctttta
    57001 tcgatgtaac actttccagt tctccttgta caactagtcg cgtgacggcg tccatgtcgc
    57061 cacaagtaat tagtgtgaca atttcttttc cctcgacttc attcagtaat tgggtatctg
    57121 taggagcaac tttttcttta aaaaatgttt tgtatgtata cacattttct aaatctgtca
    57181 aatagatctt ggcacccatc tctaaatttt ctagcggtgt aaacagcaat tcagggttcg
    57241 ttgcacgatg gcttgctaag gcataatttc cttttcccat ttcttgatct ggagataatg
    57301 ttcctgctcc gtaaagtagt gacgtattat ccaagccttt gaaaaccggt aaattgattg
    57361 aaactgaagg gactgcgata ctagcaatca ctggtagatc tgttcctctc atttgcgacc
    57421 tcatgacccc ttctgtcgtc attggttcta ccgcatcaaa gtcaaaactg acatctttat
    57481 cgttattctt tttcagatct tcttttgtta cattcgctat agcatattta tctcctgtct
    57541 ctctgacaaa ataatccttg atctgttcat tgaaaatcaa agctagtcct actaataaca
    57601 agaggaacaa gaggatattg actagccatc tttttgcttt tccttttttt ttcattttct
    57661 ctccctccag actcatttta ctgtttttct tttgctcttt ccaacagccg aatgacacat
    57721 ttctacaact ctgtgtattt ttttcgtaca cctgcgcttt tttctacagg atatttcaaa
    57781 ttatttttga tcaatttttc ttcaatgatc tcatctagat ccaggtgaag gttgtctgcc
    57841 atcatgtatg aatagatcaa tacatctgcc agttcttctt tgattcgttc taattgtgtt
    57901 tcttttactt cttccggcgt tttccattga aaaagttcta acaattcaga tgcttccaaa
    57961 gaaatagaga tagccaaatc tttttcgtta tgaaattgtc gccaattacg ttcatctcta
    58021 aactggttga ctttttccat actattcatt tgcttgcctt cttccaatca cttcttactt
    58081 gattaatcat accatttttt tacataaaaa atagatgatg cttttaaaaa aactcctgtt
    58141 ttcttttatt taaaataaat gtcatagaaa atcaataatt taggtagcca ccatgtgtat
    58201 gcagtagcgt gtcttcttcc cttgaggctt tttgtttttc gatacgttct tcttcccaaa
    58261 cagacaacgc tactagaatc tctcttaatg attttccttc ttcggtgagc gagtactcta
    58321 ctttaggcgg gacttggtta taggcttttc gatggatact ttcatcttgt tctaactctc
    58381 ttaactgctg ggtcagcatt ttttttgtga tcgtagggat ttgcttgctt ctccacgcct
    58441 aaaataagcg atattgctct tccctttatc tggtgtaagc aaagcagtta gcattttgac
    58501 gatcgtgggt tccgcttttc acttcttatc tacttagtat catgaagtat actttcgtac
    58561 taatactaag tagcggaagt gtcaactact ttgtttaact acatagcaaa agaaacagaa
    58621 gggtgacaac ggtcatcacg cttctgtttc ttttgaatat gctgtgttgt tgtctctatc
    58681 tccgtgtaga ctttcctcaa acttatcctt tgacagtaag tcagtaggct gatttgctct
    58741 ttctagatgc cagatacatt tctacaaaaa aatagtgatt ctgttttgaa aattcgattt
    58801 ttagttctgc attttcgtaa acatccaaca tttttttcgc actgtataga cctaacccgc
    58861 catttttttt agaggttttt cctcttttga ccatctccga tagcgtcaaa ttcgttggat
    58921 ttgccttgtt cgctatacta aaacgtgatc cctctttttc ttccagcaga ttgatcgtaa
    58981 tcggactctg atcggctaca cgatgatcga atgcgttaga aataaaaata gataaacagc
    59041 gaataaaaac aaccagatca atcgggattg ttttcacttc gacaggaata actacttgga
    59101 tcgggatttt ctccgattct gctttttcta gaaattttaa aaagacaccc ctgacagctg
    59161 gggatacaat atttgttagc tgttgtattt gcggtttttc aaaaatagct gcagaatgat
    59221 cttctaatat ttctaaaaat tccaacgttt cttttttatt ttcctgaaga gtaattttta
    59281 atgaaaacag tagactttta taatcatgtt tgaattcctc tagatgatgg taatattctt
    59341 catttatttg atacagttga agcgattcct gatgttgtct ttcttttact tggaaactga
    59401 tcaacataat ggaaaaaaac aaagaaaaaa aactgatgac tatcgacaaa agagatagaa
    59461 gcagataatt agatgagcgg aagtgaacac ttcccaatac caagtaaatc acgccccagc
    59521 ttaataaaat acaaatacct ccaaaatagt gttcagttgg ataagtaaaa agcaagttga
    59581 gaaatctcgt ctgtttgtct atacttttta ctaatagata gaaaataaat gcgataagta
    59641 gtgcaaaaaa acttgctgtt ccaagatgtc tctttcctaa ataatccatc agatcaaaaa
    59701 ccagaaaaaa gctgagtcca gtgatagcaa aaaaaagatt actgatcaaa cagctgataa
    59761 acaggttttt cgtagtaaaa taaaaaagaa acatcaataa taagccaact aggtaaggaa
    59821 agctcacttt agggatagga agtaaaagtg tcaccaccat caaagctata caaccaaagc
    59881 tgttttttac tagcagttgt ttcgaaaaga ataaatagat acctaaatct aaaatcaaag
    59941 taaaggtcaa ataaagtgtc actgttgcga ccatcttatt cctccattgt tttgtttcaa
    60001 aatgtacaca tattttttta ataggagcct gcatatgaaa aagtatttta tcatagaaga
    60061 taaccagttt cacagcgaat ggatcatcag agaattggtc aatcatcata tcgagaatat
    60121 cgtgcagctc acttctatct ctcaaatcaa tcagttgatc cagttgggca ctattgataa
    60181 tcacgatgtt ttctttatcg atatcgactt gaagcaatca ctcacaggta ttgatgtcgc
    60241 cgaacaaata cgtaaaaaaa atgcacatag taatattgtt tttttcaccg cttatacgaa
    60301 ttttgcagta gagatcatca atcggaaaat ccttccgatt ggatatatca gaaaagacag
    60361 gaatttatct gaacaaattc gtcaagtgct agaacagatc aattggaaag aacagcagat
    60421 cgtaaatgtc gataaaatct ttattgatca aaaaggtgga agtctcgtcc tgtatcctcg
    60481 ggaaatttta tatattgaaa gtatcaaagg tttgaaaaac caagttatgg tcaaaacaac
    60541 tacagaagat aaattagtga atacttcttt aaaacaattg aagacagagt tagaaaagta
    60601 tgcttacttt ttacttttaa aatcttactg tatcaactta tcgcaattgc agaaaattga
    60661 ttctgctcat tatgtgttag aatttttcaa cggtgactcc ttactcgttg gtcgaaagat
    60721 ctttgaaaag acaaaggaac gtttccatga cttccaaaaa actgcttcga gttgatgaat
    60781 aagagctgag acgtaagcat agcagatttc agggagtggg ccttgaatcg gctccactcc
    60841 ctgaaatcgt tgatatgatt ttacgcttcc tcttctgtag gagcatctgt taacgaccat
    60901 gtgaatgttg aaacgtagtt gtctacagca taattggata caggaacaaa taattccact
    60961 gattgtctcc cttgttccac attctctcct aatgaataaa tccatgtgcc aatcccttca
    61021 tttgctcctg cagttactag atctactgcc tgatctgctg ggatttcgat tccttcagcg
    61081 ccagttccag aaacggtaga tggcattcca ctgctattag ttgcgctatt tcgaatcacg
    61141 gcgtttttga atttcacctt tgaaccagtt agtttattcg tatcagatgt tgtaccaata
    61201 aattctgttc gtttgactga taatttccaa ccggcataat ttccgctttt atccgttact
    61261 tgtacgaaat tctgtacttg tgtttgtgct ccagtactgt ccgttccgct ttgtaatgca
    61321 gcatatactg tttttgctgt acctaacttg attgttccaa aatcaagatc tggagcttgg
    61381 tcaatcgtca gtaatccgga tgttccctga tttggttcgt caggtgtaaa aggagtatca
    61441 ggatcatttg gatcaacagg aggaactgga acaacgtctt caggatttgc agcatctggt
    61501 ttggtcacag aaatcgttcc ttctgtagtg gctgagctct tattctcagc aaaaacactt
    61561 tgtgtacata ataatagact tccagctaga acggtactta atgcaattgt ttttttcatt
    61621 tctttttcct tctttctttt aaaataaata atccagcaaa taagccaata agaatgactc
    61681 caacaagtat cagccatcgc cagtcaaatg tttctggctg attttttaaa tcaatatcct
    61741 tttcattcaa ttggtccgct gcatcatcag ttatttcaaa atcggtatgg agattccagc
    61801 gtttttcttt ccaagcaccc ttcacaaccg cttgataatg tcccggagct aatttttttc
    61861 cttctaatgg gacaagtaaa tcaaaatttg tattgggtgc tacatctatt ttttcttttt
    61921 ttattgaaag gtaaggctct tttgtttctt ttttgtagat ttcaaccgat gtagtcaagc
    61981 cgaacatata agaagcattt ggattttgaa gattcgcctt aatcacattt cttccatcgc
    62041 tttgatccgc ataagctttt tttaatttca aatctgggga agactctttt tcgctatttc
    62101 aaatcaagat tgctttcact tgagaaaact gattgacgac accaactgta cttttgttag
    62161 aaacgttctc ttttttttct tgaaacgtga tccctcctgc gaccacacca gccagctctt
    62221 tttctggcgc tttcagtgaa agtgtctcag tgaccgcact ttgaggagga acagttattt
    62281 ctcccactgt ttttgctaag tttttcatat cataacttaa tgtttcatca aatttacttt
    62341 ttgattctcc atattcgatc acaccgtttg aattcgtcgt agcggagtgg atcaatgtct
    62401 caactgttac tgatttatct gttcggttaa agaccgtgta ggggatttcc tgtgttttcc
    62461 caggctccat ccttacattt atatatgttt tgttcttatc aatctgatta gccggtaatt
    62521 caaaatctac cgcaaaattg aattcggagg catggacagg tgtctgccaa aggatgaatc
    62581 caaggagaag gatcataagt aaagagcagt tgtttcgatg acttttattc catattcgat
    62641 tcatcaatca tcctccttga tctttattct gtcggcgcat ctgctaatgt ccatgttatc
    62701 tctgctgagt aattaccagc tatctgattt tctttggtta agacaagttt aaacgcccga
    62761 ccgttattta aatattctga ataatctgct tgcacattcg ttgaattttg tcctctgaca
    62821 atttccagtt cttcttcatt cactaattga tactcctctc ctttcttgaa atataggaca
    62881 tcctgcatag aaaatcctgc atcatttgtg aaaggctgag ataattttat cgttaagatc
    62941 cacggatttt tcgtttctcg ctgatcataa atccataatt tttcctgtat atccatttgg
    63001 ccaaatgctt cttgtgtcaa accagttgct ttgatctgac caaaatcgag tttggaaggg
    63061 gcaagaaatg agagaccacc ttctactacg atcttcgtag caggtgcaaa aggcggggca
    63121 tcatgataaa caactggcgt atcacttgga ttgtgattcc caatttcatc gttggtactt
    63181 ggcttgttct gcacaccctt agcagtagct tctcctgctt ggtcgtttaa tacaatttct
    63241 aatacatctc ctgcaaaaag ttgattttct gggatagaaa gaacaaattc ccctgtatca
    63301 tcaatagttg tttctgacaa aacagcttga ttttttttca atgaaaccaa ggctcctgct
    63361 tcacctgtac ctgtgatcgt gcgagaagct gcattgatca atgtggtact taaattagca
    63421 ggttcaggag gaatcacatc gactgtcgtt ttggcagagt ccagttctgt cacttcttgg
    63481 ccacctctta cttttttagc agcaacaact tgcacttgat cctccgtttt taatctctca
    63541 tcattgggta cttgaacttc gaacaagccg ccttgttcgc cttctcccca tgggttatat
    63601 cctgcccctt cattttcatc tgtttgtcct tgcgtgagag cagtaatttc tacagatgat
    63661 ttcgtcgtat ctgcgtaagt aatacgtaaa gtcacttcta cttcgcctgt ccaagcatct
    63721 cttggctttg cctcgacacc ttcgggaaca acgacatgcc catagatttt tttatctgca
    63781 ttcgttggca ctcttaagga ttcaatcatc gggagctgat tattggcact gattcgatta
    63841 taatttttca ttccggcacc tgtctgagca aattcatttg ttaaacctgc actagggctt
    63901 gatacgaaat tgcctaagtc ggtcccattg atcgataagt ctgtcatatc agaaaaataa
    63961 tccgggtctt tttcaaagtt atcgaatgtt gcgcctgttt ttcgccataa agcgatttct
    64021 gaatgggcga tctttaaaga agaatctcca tttccttgga atacccagcc gcctatgcca
    64081 ttggtctcgc ttccaatccg tttgttttgg aaatcaaaat aggtaggaga ttctaagtcc
    64141 acattcaaca catccccact gatgatgccg gtcttatctt cccctgtttt cccgaccatg
    64201 actaaataag ttttttcacc tgccgtaaaa tttaaatcaa tatctccagt tgtatctaca
    64261 gttgctccgg aatctgcgcg tatatcgata tttgattttt ctccagtgac tgtaaaatca
    64321 gctgaacctg gactttctag aaaaccagaa gcgtcataaa attggatcgc ttgatttcct
    64381 tgattgagtc tgttcacgta agatttgcca tcacctgtat ttcttaccct caatgagcct
    64441 ccattggtta ctgtcagctt ttgcgtacca ttctcaaaac gaactgcgct ggaacgccca
    64501 ctctgcttat cgatgtcgat aactgatccg gctccttcta cgatcaaaga aaggcctttt
    64561 cctacaaaac gcatcccatt gttcccgggt tgatcgtcat ctccagccat tttgaccgag
    64621 atttgtccac cacttgatac atggatttga ctagaatctc cttgcaagcg aaatccgccc
    64681 gtagttgctg tattgatggt gattttccca ccattcgata cagtgacttt cgatcctgaa
    64741 cctctcattt ggatgattcc gcgattgttg tcattggtaa tattgatctt tgtatttacc
    64801 gaaaattcgc ttcccgaacc actgacttca aacatgacat taccaggtag cgtatcgact
    64861 gcctctcctt catcgctaac gccatctgcc cgatcataat caagagagac ctttgaaccg
    64921 cttaagatat ggattcctgg cgctgttttt ccactaccag tctcatctcc tgaatctaga
    64981 aaaccgtaga atttccctag atacggtcca taaagttgac taccattgat cacattcaat
    65041 tgatagcttc tacaaaaata ggctttaccc ggagcggcat tcagtccttc cttcactggt
    65101 tggacatcca acacaccgga aacaccatct aatgtgacag ttgcacgagg agcgtaaatc
    65161 agtcctaatt tggacccatc atacaaatca agtgtcccgg taaattttat ttctcccgta
    65221 aaatgatatc tgcttcccca aaaatcggtc atataagcaa tgataatggc atttccatcg
    65281 atatttggtg tcaatccacc tggatgtgca gctgttcctt taaaattctg gaactcgatc
    65341 gactgaggta tctcattgat ataaatatgg tgtgtgccga aattgtgagc gaatccacca
    65401 aaatcaataa cgatatctcg tttgatctct aattccctgt ctaaagtcaa aggctgaatc
    65461 agtcggatct tagttactga ttcgtctgcg agaagctgcg ccagttcttc tgttgtggca
    65521 gcttcgctgt acccatccgc tgaaacagct cttgccagtg ccgcttcact gctatcagac
    65581 gtttgttcag ctacttctga ggtctcactt gtcttatttt cttcttgcct tttattagta
    65641 gaagcctcac tttgagattc tgtagtttca gaagtaatcg attcttctga aaaagtagtc
    65701 ttgtcaaaac tttgattctc tgattgcgtt gattcgtttg ttttctctac atcggcaaaa
    65761 acggatgtca ccatcaagag agggaagaca aacaaaatcg acgcaatcag cttttttaca
    65821 cattgattca ttctccacca tcctctctga cgttttgaca attttatttt atcgttttaa
    65881 tctatttcca attgtacttt catatttaaa atatgtattt ttatggaaaa atttttctat
    65941 taggtgtaaa tttctttttt gtacaaaaaa aaggctgtga caaaagtctt gtcacagtcc
    66001 cttatcccga acaagcggtg ttctcccgcc aactcctcgg tcacaacctc cattttaaca
    66061 ataaacctgt aaatgtgagg gcaatacttt gattgtcaca ggtaatttat ccccttcatc
    66121 tccatcaaca ttcgtcgtca attcttcctc atcagctaat gacacaacta attgctcaca
    66181 agtaaggtag gctagatttt tcgtagattc atctactcct tttaataggt ctggcaatgt
    66241 tttaagcgta tcgatcaaag aagaatcttt taaataaacc aaatggagct taccatcatc
    66301 cacttttgca tttggaagta atgtttcaaa accgccgact gagttagtta gtcctatcag
    66361 caaggtactg ctctcaattt cttccttctt tccatcaact tctacgtgaa acgagtagga
    66421 ttgtgtggaa gctaactgtt tgattcctga catgaaataa gctagttttc caaatttcgt
    66481 cttcttctcc gcatctacat cattgattga ttcagggata gagccaattg cgacgacatt
    66541 cataaaatag tcgtcattta tttttccaat atccaatggc ttgactgatt caatcgaaaa
    66601 atgattgatc gcttcctcag gttctagcgg tatccctaat gctcgagcca agtcattcac
    66661 cgtacccaat gggaaaaatc cgaaatttgg ccgatgttct tgttcagcga ttccactgat
    66721 tccttcgttg actgttccat ctccgcccat gacaaacaca ctatgatacc cttccgtagc
    66781 cgcttcgcga gtaaagtttt ttgcatcccc tgcttttttt gtatgcaaga cgaccacttc
    66841 atcaaacacc gattccaatt ttgctatcgc taattgctca aattcctttg cttgttcacc
    66901 gcctgaactg gggtttacca ctaataaaac ttttttcatc actttctttc ccttctgatt
    66961 gatttcttta atataacgat agcataaaga aaaaaattag gctgtgatga agtactgttc
    67021 atgaaacatt catttatttt ttgacaagat aaaacatcca tactatgaaa ctgaggaaaa
    67081 ttaaaacaat gatataggaa ataaaagaaa atgataggca ttgtatacct aataaagtaa
    67141 aaatcttagg tggcagataa aaacacaaaa atagcaagag aatgtaatag cgtttcttcc
    67201 actttttgtt atcagacctt atagcgatca gaggaatacc aaatgcataa ctgaaaaact
    67261 cttgatgtac tgttaatatg tcacggcctt gaataataaa tgcgacaatc cccgctataa
    67321 ttagtgtgcc taacaccaaa acggaaaatt gatgaatata ttttatatac aaatgatcca
    67381 ttgtacttcc tgccttttta tttgtgagaa aaacaaatcc gtatacgtga ataacttcta
    67441 gaaataaaag aaatttgcaa aaccactcca tttatctact tctacgtgca ttttttgata
    67501 ttgttttctt tatttaagat tatacagaaa aatatctagg gtcgctggat tctaagtaaa
    67561 aaaaattttt aaaataaaaa gaactaaatc aagtagtaat taaactactg ttgatttagt
    67621 tctaataaaa agatttagat gaacagtttt attgatttaa atagttcata ttttttattt
    67681 ataccgctta tttactactt gctgtactag aatctgaagt gaatgtactc aaaacatttt
    67741 caaagtcttt gtctttgatt tttacattag cgtctttcaa tacttcgcca attactttat
    67801 tctggaaggt actgtcactt aattttgtgt ctgttgcaat ttctttcaat tcttttttgt
    67861 atttatccat gtcgttgcct ttgttttggt ttttcaccat tttaaccaca taatattccg
    67921 tagtataagt tgaagcattg gtagaagtga tcacatcaga aacttgacca tctttcaact
    67981 taaaggctgc ttctttgact tctgccggaa ccgttgtagt agtggaatca aatttcactt
    68041 ttccgccatc ttcttttgtc gtagtatcag ttgacttgtc tttggctaat ttagagaaat
    68101 cgtctccctt atcagcagct ttcttcacat cttttgcatc atcttcactt gctacttgga
    68161 taatttgagc ttccacttct ggatggaaag attcccaagc cgtttttaaa tcttcatcgg
    68221 tcaatttgat atgtttcttt agaccttctt gaaaggctag actttgtttg atactgtctt
    68281 tgaatgtttg ttctgtgtaa cctgcagatt tcaattgtga atcaaatgaa tcacccaatt
    68341 gtttctttgt ttgatcatat tgagaatcga tttctttggt tgatacgtca tctccatatt
    68401 tatcttcaaa tacttgataa acgatcaact gttgcacagc ggaatctgct ccttgcattt
    68461 ttttcaagct atccattact tcttgttcgc tgattgtacc gcctttataa ctaacggctg
    68521 ttttttcacc ggcattcgta cagcctgaca ataagccagt gcctagtacg atggcaagtg
    68581 ctaaaaatct ttttttcatt tatcaattct cctacttttc gttttttgcg aattgctaaa
    68641 aacaaaggtg ttcaagatga aaatcatcaa gaacaccttt actaatctgc tcgatacgtc
    68701 ctgaatagat ataaggctac acaatccaga aagttcgctc agcagaataa atgtcaagta
    68761 gcacatcaca ccttaatata ctacttcttt tgttaccgtt tttcaagtct atcttgcgtt
    68821 ccgtatttaa cgtaactaat tcttagaaca aatgttatcg attttgtttt tcttcttccg
    68881 ctgctgggat attcaaatca gacactttta tgttcgattt tccagacatt tttttgttgt
    68941 ttttattatg atatattttt atacaactaa aaatgaatat attaaaacta ccaaaaataa
    69001 cttttaacgc tgtttcatta tatgtttttt ctattttata taaacttttg gatacttaat
    69061 accaaccggt tggattgtac caacatgaga atgtgtcagt tctatttcct gtcgcacttg
    69121 cataggccca tactcctttt gttgcatttg ctgatcgaat agagtttcca actttcgctt
    69181 ttgcaccgtg acgattatag acacgactat agtggttaga atgacctgtt tttctcgtac
    69241 aagttgtatc actataataa acccatcttc cagagctatg attaattgct gttctagaaa
    69301 tttcccggtc tactggtaaa tttgacttaa taatattacc tttagcatca aaatgaatct
    69361 ctcctggctt taaagatttg taatagattt caccgtgaac tgaatttgct tccacttcag
    69421 atgacatagc gtttcctcca acaaaaccta tccctagtgc acaaataata actgcttttt
    69481 tcataaaacg acatcctttc ctttttgatt tttaaagcat gatcttcttg tttcctattt
    69541 tggtcagata tggatcatgc gtgactacga taactaacgt accttgggaa gcttgttgcg
    69601 ataagatttc aactacttta tccttatttc ttttatccaa tgacccagtc ggttcatcgg
    69661 ctaaaatgat tttgctattc ttcaataaga ctctcgcaca ggcgattctt tgcttctcac
    69721 caccagataa tgtgtagata ggcgtactta gcttcttgtt tatatttact tttttcaatg
    69781 cctcatactt tgctgttcgc ttatctgaaa catttaaatt cgaaaacttc aatccaatat
    69841 ctaaattaaa atcgattgtt tcatattcaa tcaacccaaa atcttgaaaa agaaaagata
    69901 tggtatttct ttttatcaag cgcttctctc tcccagttaa ttgtttcgtc tcttgatcga
    69961 acaaatagaa ttgtcctgaa ctgaactgat cattcaatcc taatatattc aataacgtac
    70021 ttttacctgc tccactttct ccaacaacag tcacaagatc cccagcatcc gcagtgaaac
    70081 gatccaaatg aagaatcgtt ttgtttcgaa tgattttttt cgcacctttt atcactatca
    70141 acggctccaa ttgttaacct ccattcatca gcaataactg acttttcttt tctgtagaca
    70201 aaataaaaac aactgaaaga ataaaatcaa tgaagctaaa gaaaaaaata ttaaaaagat
    70261 atttaggctc tctttctata agaaaaagga agattacact tgtcgttccc catttgatga
    70321 ggttcatcat gaagaaatct ttatacgttt caaagagcga ataaccactt gaacgaaaga
    70381 tagcgatttt ccgacaattg tagacaaaat atatgcataa catgacatag ctcaacaaga
    70441 agaaagaaaa gaaagtgagt aaaaatatct gtatcaagta atgaaatgat ctgcttaaca
    70501 ctttgacagc tatctcttca gtctgagtat acggaaatga taactgcaag ttatcacgta
    70561 actgactttc ttcaatcagc tcaaataacc tttctttcga caaagagcct atcttgatct
    70621 ttaacggtgg atagatttct ccgctgagaa tatttctttc caaatacata ctgtttttca
    70681 atgtcagtat ttcaacaacc ggataatgtt cgatccaggg gatagaagga ataaatgtat
    70741 aaatgggttg attgtttttt ataaatagaa atttcggttc tttttcactg atttcttgat
    70801 aatattctgt tatttgcgcc aggtcatttt ttaaatctgt atgtctatat cgttcaggga
    70861 tcaatataac agggtgcgtt tctttttcgg ttatttggat caaccttcca gagacatcaa
    70921 tcaaaggaaa tttcttaaga taattcagat ttatttttat ccctctgcca aactgttcat
    70981 tttcttcatt caagtaatct gatacattga ctaatatact tccgttttta ttcaaagttt
    71041 catataattc ctgatctgct gctgatgcaa aacgattgga ataaataaaa tccacgtggt
    71101 ttttcccgat ggttactgga taaaatacat gatagtcctc accagcgatt tcttttggtg
    71161 gttcatggga aacagctaac tgtgaagaag aaaaaacgat agttgctaat tcgacattgg
    71221 caacggtcaa aagaagagaa aacaacaatc caaatgtaaa aagaatgtat tggatatagt
    71281 tgaacgcttc tcttaacgaa gcgatttttt caactaataa aagagaaatc aaagagatac
    71341 catatattcc gctgaacaag aaaacgtaca tctggaacaa ataaagaaat tgctccttat
    71401 aatacataaa aataaaacca ccaatgattc ctgcaattat gaatgtaata atgattttct
    71461 ttttccccaa taaacaccac aatacttgat gtaatgaaaa gtcattattc aacaataaac
    71521 tgatttcttt attcatcaat aagaaaaagg atgataacaa aaggaataaa agaatcaatc
    71581 caatagttaa atacactcga taattatata cgtctgtatt tgaaataacc gaatacgaat
    71641 agctattttc aaagtcttca aaaatattag tggcataatc agtgtgaaat gcttgattga
    71701 atcgatcagt gaatagtgaa atgaaccgtt cataggcttc tttttctctc gtcatcaaga
    71761 aaaattcacc ttcaaaatct tttattttga tcgattcttc aatggacgtt acgcgaaagg
    71821 aggtattaga atagggtacc ttctcgtgag aagatttgac atccccttga taatacaatg
    71881 tcatttcaag ctttggctgt atcttgaagt tccaagatgt atctacttca ttggattcat
    71941 ttactgtttt tttcatataa gaaagctcag tttctgatga gacattttca aataccatga
    72001 ttagctgctc atattcttct tgcccaataa aatgttgtgg caatcgaatc ggagtataag
    72061 tatctgtttg atggaaaaat tcatttgtcc gatagagtac ctgtttattt atactaacca
    72121 aggcaaaacc actcatgata ccggtcacta tgattgtgag taaaactgtg aacttttttt
    72181 tgattatcac cactctccct tacttaaatg attctttgat aaaatcatac cgtttttcat
    72241 caaaaaaacg tatctatcaa gtgtataggt catgtagaat aaatctatat actgtagaat
    72301 cgaaaggaga agaagatgaa caccggagaa aaaatacgaa cttaccgcag aaattgtaag
    72361 cttacgcaaa aagaattagg cgaaaaaatt ggtgtttccg ataaaacgat ttctagttgg
    72421 gaaaattctc gtacaatgcc agaccttgaa atgctgtcac ttttacatca tactttaggt
    72481 ctgccgattg attttccttc tattgaatca gctaccgaat caatagaacc aacattgcca
    72541 gatgaacctc ttgttaacaa gaaatcttat tcatcaaaac aacccttctc actcaaaaag
    72601 acactatctt tctttctgct cgtcttttgt ttggtctatc tttttcttat caattatcaa
    72661 tacaaaaatg aaacgatcga tcgattcaat ccttttttga aaccacaaat cggctatacc
    72721 acactccctg taggatacgt ggacaattcc atcgaacgtt gggtatgtaa tgatatcttt
    72781 ggccatggta catggctgtc ttttcacgct ggagaggtag aaaaagagaa aaaccatgct
    72841 gtaatcaaac acaaaggaaa acacgtcatg aacgttcgcc tgctcacttg ggaagccatt
    72901 ccctcagatt accaagaaat cctcagccaa gactatgacg acgccttaaa aagaagccct
    72961 caggagccag ataggcatta agtaggagat gaacgtagag atagaaaaaa tcaatgcaca
    73021 tgatcttatc caaaaaacag gggtacaaat taattgaaaa agccttgaga tgaaagttcc
    73081 taaataaaaa agttcagaat gataggtatt tctggcttat agtataatat ttacgccaca
    73141 gcctcccaat gattcttttt ggaatcataa tcaaggatgg acattcacaa aaatattttg
    73201 ctcattcgat ccgaatagat taacgaccaa tctagtaagt aatatagatt ggtacgtagt
    73261 caagctgcac ataatttaga tcaattttag ttctttttta tatttctatg atctgctcat
    73321 ttacttttat atgatggctt acagtaacat aaataaatat gataatcgct ggtagtttat
    73381 ccaagagtac tcaataaaat acaaaatgga ttgttcaagc gcctatcata atgataaaat
    73441 gaaacaaata aacgatacaa ggaggaagag actttgagta atggcagaac aattttttca
    73501 ttgtaaaaat tgttcagttg ttaatctatg ggatttcggt agcatgggca tttttttata
    73561 gaggaatcac accaacctgg ccgttttatc tcttgttagt gggaatatta gtaggaaatt
    73621 ttgcccctct tcactatcgt acgaactttt ttccaactga ttctggcctt tttcttaaaa
    73681 caaagacgcg aaaattagaa aacattattg aaatgacaat tacctgtata atcattttct
    73741 tagttatatt cctaaacgtc ttctaataag aatctcttaa aattagcttg ttacagttag
    73801 ttacctagtt tatataacac agttatctta tcttccttat caacatcttt tgccaactct
    73861 ttgtcttaaa ttcgaaacat ataagcagag ctacgaacac caagactgtt tcggctctgt
    73921 tttttgcatc aaattcactc ttatatagct gataatctcg gctctttaaa agaaattatt
    73981 ttcgcttcat tttattataa aaaggctagg aaccttgatg ttatcaggtt cctagccttt
    74041 atttaagtat taccccacag acccttccat atagattttt aaattacaca ctagtttaaa
    74101 ctaaaacaaa caaggaaaaa cttaccattt aatcaaaaaa ttagctcata tgaatatatt
    74161 agaatgtaaa ttagactggt caaaatctgg tcaaaaaaga ggacgaatac aggtagtcct
    74221 cttttttgtt attttgatat tctaaatcat tttttattgg attattccct tgttcgatat
    74281 agacccaaat tgatctatca ataattatca aactgagtca tatagagaca aaaatttcaa
    74341 aaatgacctg ttaaatggtc atcgaatatc atttaaaatc aatacgacgg agtcagacac
    74401 ggagtcaaaa caagcccgct gagagtaaaa aatatacagt cccataggta aagtgccaca
    74461 ggactgtata tttttttatt tgagattacc tgaaatgatt ccatttaatt tttatctcat
    74521 tttttcctac tagttagtat caaatactct cctattttct caataaacta ttttgggtta
    74581 catggtatct gaaacagcta aaaccttgtc attatagcaa aaaatcactt tatctccgtt
    74641 tcatttcaga tacatttcac ttaagcggat tccaaaaaaa caaaagaaaa aggaacccct
    74701 agttagccta tgctttttat tggttcctct caaaacacgg tcatataaca acggggtggg
    74761 gtaaatgaat gaaaaaggtg gaggaaacat tattcaatag ctgtcttaaa tacttcgtta
    74821 taaaatgtga acaaataaaa gacatctatc cctattttct acacttccac ttgactacca
    74881 tctttgaatc gtacaatttt cttaactctt ctcgtttacc tcaattcatt ctcaagtgga
    74941 atcaactcat catctaacat tttacgccat gctcgcttca tttcttgtgg gtatttaaat
    75001 ggcttcgctt ctggatgttt ggctttatat ccttcgtaat ccttactgtt gataattgct
    75061 ttcatattag gaattggatc ttcacctggt agatattgca gattagaagc atgtaattct
    75121 ttctcagaaa caaaccactc attggaaaag acatgaatca aatcatcact cgcttgtgta
    75181 aaatagcgac gtttaatgac aaagatatct tcattactat ctaaagtccc cacatcaaat
    75241 gcatttaaaa tttgtcggta aacttctttg actccttctg ttttattcaa tttagccaaa
    75301 gctttttcaa tttcttctcg tgcatctgaa ctattcgcag cataagttcc tagtaattga
    75361 tcaatataag ttgaatcaat atcatacaac ttagtggtat tttcactata aaattcgatc
    75421 gttgaaaaat cttctccttc tttttctggt tcttcctcta ttaatgaacc cttcacggtc
    75481 tcgtacacgc caatttgttc ttccaaaagt tgaatttgat tgttcaaagt tggagatttc
    75541 accatgtcat cattgtaatc gtcataagtc actaacgatt cataagcacg attcaattct
    75601 tgaaatgctt tgacgtactc gatacggatt tcaagtggcg tatgttcatc aatggattcc
    75661 atggatgaaa cctcttttaa cttaccatat gcttgtttga atcttttttt ggattcctga
    75721 taacttggat aaatcaaacc tgattcttct tttgcttttg aatacagttt tgtcgcatcc
    75781 tcgacattct tctccattgt cgctggctta cgaaacgtca caatcaatcc ttttgtctta
    75841 tcaggatacg tacgattcgt acgtgaaaaa gcttggatca atcctgcata ctctagatta
    75901 cgatccacaa atagggtttg aatcgtaggg gcgtcaaaac ctgttaatag acgatccact
    75961 acaatcgcta aatcgatttg tttcccaaat tgtttgaatt ccgctctttt tcgtgccaaa
    76021 cgattattga tgtctccgtt ataacgctca atgtctgcta atgaccagga agtatcataa
    76081 tactgattat attcctcaat aattttttgc atttcttctt gttgggcact tgaatctcgg
    76141 ctattttctt ctaatgaata ggtaatagcg attcgtggaa aatcaggatc ctccatcgtt
    76201 cgtccttcac gaatgagctg attgggaaac tcttttgtta accaatcagg ctctttcgtc
    76261 atctctttga tggcccgata atactttttg gccatgtcaa tggaactcgt ggttaagatt
    76321 gcagatttcg ttggcctgcc atttcgaaaa tcaaattttg tataggcatt atctggacgg
    76381 aacattttat gaatgacttt ttggatatgt tcatctgctt caaaaacagc tgggtccaaa
    76441 taactttctt ttttgacagg ttccatttga tcaatcatac gattgatctg ttctgatgaa
    76501 tactctgcat atgtttctac ttcttttaat ttccgataga ttttattctc tagtgaagtc
    76561 ggctccacgg tgttttcatg ctctacttga aatcctaaga cagagccatc ttctagcgca
    76621 ttcttaatgg tataggtatg caagacctct ccatactgat catgggttgt acgtgccaat
    76681 tgccctttgg cttgtttccg attctcagga aaaatcggtg tacctgtaaa gccaaaccaa
    76741 gtggatttcg gaaacatttt tttgatttct tccatatttt cagcact
//
LOCUS       DARBFO010000006        73120 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000006 DARBFO010000000
VERSION     DARBFO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 73120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.006"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(176..1750)
                     /locus_tag="SCK51_000511"
     CDS             complement(176..1750)
                     /locus_tag="SCK51_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEG1251160.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(2005..2514)
                     /gene="mreD"
                     /locus_tag="SCK51_000512"
     CDS             complement(2005..2514)
                     /gene="mreD"
                     /locus_tag="SCK51_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEG1251161.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(2518..3372)
                     /gene="mreC"
                     /locus_tag="SCK51_000513"
     CDS             complement(2518..3372)
                     /gene="mreC"
                     /locus_tag="SCK51_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEG1251162.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(3527..4165)
                     /locus_tag="SCK51_000514"
     CDS             complement(3527..4165)
                     /locus_tag="SCK51_000514"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEG1251163.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(4166..4816)
                     /locus_tag="SCK51_000515"
     CDS             complement(4166..4816)
                     /locus_tag="SCK51_000515"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEG1251164.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(4829..5728)
                     /gene="rsgA"
                     /locus_tag="SCK51_000516"
     CDS             complement(4829..5728)
                     /gene="rsgA"
                     /locus_tag="SCK51_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEG1251165.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(5893..7962)
                     /gene="pknB"
                     /locus_tag="SCK51_000517"
     CDS             complement(5893..7962)
                     /gene="pknB"
                     /locus_tag="SCK51_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEG1251166.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(7959..8699)
                     /locus_tag="SCK51_000518"
     CDS             complement(7959..8699)
                     /locus_tag="SCK51_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEG1251167.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(8712..10070)
                     /gene="rsmB"
                     /locus_tag="SCK51_000519"
     CDS             complement(8712..10070)
                     /gene="rsmB"
                     /locus_tag="SCK51_000519"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEG1251168.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(10070..11008)
                     /locus_tag="SCK51_000520"
     CDS             complement(10070..11008)
                     /locus_tag="SCK51_000520"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEG1251169.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(11001..11492)
                     /gene="def"
                     /locus_tag="SCK51_000521"
     CDS             complement(11001..11492)
                     /gene="def"
                     /locus_tag="SCK51_000521"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEG1251170.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(11507..13915)
                     /gene="priA"
                     /locus_tag="SCK51_000522"
     CDS             complement(11507..13915)
                     /gene="priA"
                     /locus_tag="SCK51_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEG1251171.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(14168..14473)
                     /locus_tag="SCK51_000523"
     CDS             complement(14168..14473)
                     /locus_tag="SCK51_000523"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG1251172.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(14478..15092)
                     /gene="gmk"
                     /locus_tag="SCK51_000524"
     CDS             complement(14478..15092)
                     /gene="gmk"
                     /locus_tag="SCK51_000524"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEG1251173.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(15579..15863)
                     /locus_tag="SCK51_000525"
     CDS             complement(15579..15863)
                     /locus_tag="SCK51_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251174.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(15881..16771)
                     /locus_tag="SCK51_000526"
     CDS             complement(15881..16771)
                     /locus_tag="SCK51_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEG1251175.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(16791..17009)
                     /locus_tag="SCK51_000527"
     CDS             complement(16791..17009)
                     /locus_tag="SCK51_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEG1251176.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(16979..18679)
                     /locus_tag="SCK51_000528"
     CDS             complement(16979..18679)
                     /locus_tag="SCK51_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEG1251177.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            18946..19719
                     /locus_tag="SCK51_000529"
     CDS             18946..19719
                     /locus_tag="SCK51_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251178.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(19800..20369)
                     /gene="thiT"
                     /locus_tag="SCK51_000530"
     CDS             complement(19800..20369)
                     /gene="thiT"
                     /locus_tag="SCK51_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEG1251179.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(20435..20537)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(20543..21514)
                     /locus_tag="SCK51_000531"
     CDS             complement(20543..21514)
                     /locus_tag="SCK51_000531"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEG1251180.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(21608..22981)
                     /gene="glmU"
                     /locus_tag="SCK51_000532"
     CDS             complement(21608..22981)
                     /gene="glmU"
                     /locus_tag="SCK51_000532"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEG1251181.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(23484..24302)
                     /gene="purR"
                     /locus_tag="SCK51_000533"
     CDS             complement(23484..24302)
                     /gene="purR"
                     /locus_tag="SCK51_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEG1251182.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(24469..25293)
                     /locus_tag="SCK51_000534"
     CDS             complement(24469..25293)
                     /locus_tag="SCK51_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG1251183.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(25266..25946)
                     /locus_tag="SCK51_000535"
     CDS             complement(25266..25946)
                     /locus_tag="SCK51_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG1251184.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(25969..26907)
                     /locus_tag="SCK51_000536"
     CDS             complement(25969..26907)
                     /locus_tag="SCK51_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEG1251185.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(27152..28015)
                     /locus_tag="SCK51_000537"
     CDS             complement(27152..28015)
                     /locus_tag="SCK51_000537"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEG1251186.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(28157..30730)
                     /gene="mprF"
                     /locus_tag="SCK51_000538"
     CDS             complement(28157..30730)
                     /gene="mprF"
                     /locus_tag="SCK51_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEG1251187.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            30871..31677
                     /gene="proC"
                     /locus_tag="SCK51_000539"
     CDS             30871..31677
                     /gene="proC"
                     /locus_tag="SCK51_000539"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEG1251188.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(31756..33312)
                     /gene="rny"
                     /locus_tag="SCK51_000540"
     CDS             complement(31756..33312)
                     /gene="rny"
                     /locus_tag="SCK51_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEG1251189.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(33723..34772)
                     /gene="recA"
                     /locus_tag="SCK51_000541"
     CDS             complement(33723..34772)
                     /gene="recA"
                     /locus_tag="SCK51_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEG1251190.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(34881..36122)
                     /locus_tag="SCK51_000542"
     CDS             complement(34881..36122)
                     /locus_tag="SCK51_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEG1251191.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(36362..36940)
                     /gene="pgsA"
                     /locus_tag="SCK51_000543"
     CDS             complement(36362..36940)
                     /gene="pgsA"
                     /locus_tag="SCK51_000543"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEG1251192.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(37149..38072)
                     /locus_tag="SCK51_000544"
     CDS             complement(37149..38072)
                     /locus_tag="SCK51_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1251193.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(38811..40097)
                     /locus_tag="SCK51_000545"
     CDS             complement(38811..40097)
                     /locus_tag="SCK51_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEG1251194.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(40090..41358)
                     /locus_tag="SCK51_000546"
     CDS             complement(40090..41358)
                     /locus_tag="SCK51_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEG1251195.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            41610..42071
                     /gene="mscL"
                     /locus_tag="SCK51_000547"
     CDS             41610..42071
                     /gene="mscL"
                     /locus_tag="SCK51_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEG1251196.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(42131..42577)
                     /locus_tag="SCK51_000548"
     CDS             complement(42131..42577)
                     /locus_tag="SCK51_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1251197.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(42670..42915)
                     /locus_tag="SCK51_000549"
     CDS             complement(42670..42915)
                     /locus_tag="SCK51_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEG1251198.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(43030..43608)
                     /locus_tag="SCK51_000550"
     CDS             complement(43030..43608)
                     /locus_tag="SCK51_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1251199.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(43685..44215)
                     /locus_tag="SCK51_000551"
     CDS             complement(43685..44215)
                     /locus_tag="SCK51_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEG1251200.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(44219..45079)
                     /gene="rlmB"
                     /locus_tag="SCK51_000552"
     CDS             complement(44219..45079)
                     /gene="rlmB"
                     /locus_tag="SCK51_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEG1251201.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(45072..45467)
                     /locus_tag="SCK51_000553"
     CDS             complement(45072..45467)
                     /locus_tag="SCK51_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEG1251202.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(45464..46876)
                     /locus_tag="SCK51_000554"
     CDS             complement(45464..46876)
                     /locus_tag="SCK51_000554"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEG1251203.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(46873..47409)
                     /gene="cysE"
                     /locus_tag="SCK51_000555"
     CDS             complement(46873..47409)
                     /gene="cysE"
                     /locus_tag="SCK51_000555"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEG1251204.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(47495..47734)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(47731..49191)
                     /locus_tag="SCK51_000556"
     CDS             complement(47731..49191)
                     /locus_tag="SCK51_000556"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEG1251205.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(49361..50518)
                     /locus_tag="SCK51_000557"
     CDS             complement(49361..50518)
                     /locus_tag="SCK51_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEG1251206.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(50552..51922)
                     /gene="radA"
                     /locus_tag="SCK51_000558"
     CDS             complement(50552..51922)
                     /gene="radA"
                     /locus_tag="SCK51_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEG1251207.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(51985..52512)
                     /locus_tag="SCK51_000559"
     CDS             complement(51985..52512)
                     /locus_tag="SCK51_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEG1251208.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            52742..53548
                     /gene="proB"
                     /locus_tag="SCK51_000560"
     CDS             52742..53548
                     /gene="proB"
                     /locus_tag="SCK51_000560"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEG1251209.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            53545..54792
                     /locus_tag="SCK51_000561"
     CDS             53545..54792
                     /locus_tag="SCK51_000561"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEG1251210.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            54868..55212
                     /locus_tag="SCK51_000562"
     CDS             54868..55212
                     /locus_tag="SCK51_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251211.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(55329..55655)
                     /locus_tag="SCK51_000563"
     CDS             complement(55329..55655)
                     /locus_tag="SCK51_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251212.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(56270..57472)
                     /locus_tag="SCK51_000564"
     CDS             complement(56270..57472)
                     /locus_tag="SCK51_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1251213.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            57589..58104
                     /locus_tag="SCK51_000565"
     CDS             57589..58104
                     /locus_tag="SCK51_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1251214.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(58303..58749)
                     /locus_tag="SCK51_000566"
     CDS             complement(58303..58749)
                     /locus_tag="SCK51_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEG1251215.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(58796..60274)
                     /gene="msr(C)"
                     /locus_tag="SCK51_000567"
     CDS             complement(58796..60274)
                     /gene="msr(C)"
                     /locus_tag="SCK51_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEG1251216.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(60628..61257)
                     /locus_tag="SCK51_000568"
     CDS             complement(60628..61257)
                     /locus_tag="SCK51_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG1251217.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(61241..61705)
                     /locus_tag="SCK51_000569"
     CDS             complement(61241..61705)
                     /locus_tag="SCK51_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1251218.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(62192..63460)
                     /locus_tag="SCK51_000570"
     CDS             complement(62192..63460)
                     /locus_tag="SCK51_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG1251219.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(63691..64428)
                     /locus_tag="SCK51_000571"
     CDS             complement(63691..64428)
                     /locus_tag="SCK51_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1251220.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(64495..65064)
                     /locus_tag="SCK51_000572"
     CDS             complement(64495..65064)
                     /locus_tag="SCK51_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEG1251221.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            65192..66064
                     /locus_tag="SCK51_000573"
     CDS             65192..66064
                     /locus_tag="SCK51_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1251222.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            66406..67278
                     /locus_tag="SCK51_000574"
     CDS             66406..67278
                     /locus_tag="SCK51_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1251223.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            67275..69233
                     /locus_tag="SCK51_000575"
     CDS             67275..69233
                     /locus_tag="SCK51_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1251224.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(69565..70416)
                     /locus_tag="SCK51_000576"
     CDS             complement(69565..70416)
                     /locus_tag="SCK51_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1251225.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            70539..71006
                     /locus_tag="SCK51_000577"
     CDS             70539..71006
                     /locus_tag="SCK51_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEG1251226.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(71269..71751)
                     /locus_tag="SCK51_000578"
     CDS             complement(71269..71751)
                     /locus_tag="SCK51_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEG1251227.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(71851..72714)
                     /locus_tag="SCK51_000579"
     CDS             complement(71851..72714)
                     /locus_tag="SCK51_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEG1251228.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        20468 a        15660 c        13123 g        23869 t
ORIGIN      
        1 aaacatttga atgtcgatct tacccaacca gatttttggc aagcaggtat cgacatggtg
       61 actgaagata tccaaacgtt catggaattg acagaagaat ttatctgatt tctattttat
      121 cacaataaaa aaagagttag tccagaatgg gctaactctt tttgattaat cattcttaca
      181 tgctgacagc aaagtcaggg gcaaaccatt gaactgaacc aacttttacg ctttcgcctg
      241 gttgaggagc atggatatat tgtcctccgc ctaaagcaat cgctacgtgg taagttccgc
      301 cttgtgaccc ccagaataac aagtctccag cttttgcttg agaaactgaa atttttgttc
      361 cagctgattc ttgaggaact gtccaaccac cgatgtcacg accagttact tgcatgtaaa
      421 catagcgtgt gaaccctgag cagtcaaagc cacttggatc tttaccgccc caaacatatg
      481 gagtaccaat atatttgtaa gcttctgcta cgattgctgc accattcaca cttccagaag
      541 gagctggagc aggtgttaca ggttttggtt gttcaggtgt tggtgttggc gttggagccg
      601 gagtactact tcctgtgccg tttcctgtat caacactttg atctgttgat ggtgttgtag
      661 gcgcaggtgt tgttgattct tctgttgttg aagattcagg cactgttgtt gattcttctg
      721 ttgctgaaga ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcgctgttg
      781 ttgattcttc tgttgctgaa gattcaggcg ctgttgattc ttctgttgaa gattcaggtg
      841 ttgttgattc ttctgttgct gaagattgtg tagctgatga tgattcttca gtcattgaag
      901 attgtgtagc tgatgattct tctgttgctg agctttgtgc agtagatgaa gcttgagtag
      961 cttgtgcttc agcctctgct tgttcgcgtg cttctttttc agctttttct tgtgcagctt
     1021 gttgacgagc ttgttctgct agacgttgtt gttcacgaat acgagcttgc tctgcttcag
     1081 cttctgcttt ttgacggttc aagtcagctt tcttgtcttc agcagttgct tgttcagcag
     1141 ctaatgaagt tttcagtacg ttcaaatcag cttgtttaga taaaagatcg cctttttgag
     1201 attccaaagc agcttggttt tcagctaatt cttttagttt agcatcgttt tcagcttttt
     1261 tgtcttctac agctttttta tcttgttttt gttgttccat caagtcgttg tttgctttta
     1321 ccatcgttgt cattgcttga acgcgtccaa tagcatctgc taatgaatca gcattcaata
     1381 cagcatcgat gtagtttgag cttgtgttgc ttacttgtgc ttcgcgtgct tgtttttgga
     1441 ttgtatcttc acgtttttca atacgttctt gtaaatctgc aatgtctttt actaattgtg
     1501 cagattcttg acgcaatgta tcttgttttg ctaataggtc ttgtgcttgt gtgttgattt
     1561 cagaaacttg gctttctaag gcatcgattt gtgattgtgc atctgcttgt tgatttttca
     1621 agtctgcaat cttttgatct tgctgttgta tctgagaatc aaaatcgtcc gccgcggcag
     1681 cgatcggtga tgctacggca gtcaaggtca ttgaacatac cattactgct gatattaaac
     1741 tctttttcac tcttcattcc tccgactggc ttaatttaat aaatatctaa atatatttaa
     1801 gtctttctta acgatataaa aattgtacca tagcagtgtg tcaacgatat aatagttatg
     1861 ttacaaaaat gtttcaaaac aacttttaaa aatgacaaaa ctgttcgttt cagaaaatga
     1921 tcatgaaaaa gccgcgttaa gtctagcatt tcttaagata acaacgattc tttttcgctt
     1981 tcgacctaaa aataaaaaaa gaaattattc atcactgaat aatttcttaa agggaaaaat
     2041 gaataataca aatataatca tgttcaacag cagtgtaggt cctaaaaacc ttgtaataaa
     2101 atacgtgtta tttactactg ctaatttaaa aatcaactgg accaccatcg taaacaattc
     2161 ataacccgtg acaaagatga tcatactgaa aaattcagta aagatgttga catggataac
     2221 gtctttcatt acatacatca accatacaat taaagggaga gctactgcat agataccgat
     2281 aacgccaata tagtaagcgt catagatagc cccgagaaca atcgtggtaa tcagtaaata
     2341 acgtttttca aacgccatac tgcaacataa taatgccaat atcagaaagt gggcatttga
     2401 catataggtg cctttcgacc actcccctag cattcttgtt aaatgaccat ctatcaacat
     2461 caaaaagaac aaaacgattg gcagatagta cttcattgtt tctttcttca acatggacta
     2521 atcctccgct agccgttgga caatcgtcac aactgacacg tcatacattt ctgcataagg
     2581 tttcacataa acttcccgat ccagcccata actatctggt ttcgttttaa taaccgtacc
     2641 gatcggcaag ttagctggag aattccctcc aagaccggat gtctggacaa catccccttc
     2701 tttgatatcc gtatcaccag ttaattgggt caccactaag gcatggagct tttcatcata
     2761 gtttttaagc aaaccaaacg cttcgccatt agccgaagat acccgtactg gaaaatgatt
     2821 ggagctttca ttagaggatg acagtaattc gattttagac gaagccgtat tgacctcgat
     2881 cactcggcca atcaatcctt tttgcgacat gactgccata tttgcttcga taccgtcttt
     2941 agaacccttg tctacgatca gcatgtcctg ccaactgtca ggcgaacgag taatgacatt
     3001 tgccgttact ttttcgtaac tagtcagcgt ctgattcaac tcaagttctt ttttcaacgc
     3061 ttcgatttct ctctcgtaat tcttgttttg ctgtgaaacg tcatcatagc tatcgatttt
     3121 ttcttttagc cgctcatttt cggcataagt tgaaaaaaga ttatggacag aatctactcc
     3181 atttcgtacc caccgtactg gggcagagat tgttttatca accattgcaa cactgtcatt
     3241 aacgatggat tggaagaaat tgggctttcc ttcattcgct cgctgcgcag cggtcacact
     3301 tagtatcgtc accacgatga taacaataac caaggtaata ataatatttt tattaggatt
     3361 gaacttcttc acaaagacac ctcgaattaa ttagactctt gtgttcaatt ttatcactaa
     3421 atgccgtgaa acaaaaggta agttgctcct cttaagcttt tttttagcaa ataatagatt
     3481 ctgtcatttt tagcctgaaa gacaaatttt tttatttgtt tatggatcaa tctttgcttt
     3541 gaataactgc aatcatacca tctgaaaaag aaaattctgc ggtatctgaa agaaattcgt
     3601 tacttgcata agatgtcgga tattctactt ctttattttc caatacatac ttgctgcctt
     3661 ttaatgtaag attcgagacg ggagtcaagc agcaataagc taaatacttc atatctggta
     3721 tctgtcttac tatatgctct ccaggcaaat aataagacaa ataattttga caatctctga
     3781 taataagctg atgggcaaag ggtttgaagc gaggttccat acctaaccag agattggcta
     3841 aaagatgatc caatcttccg cccgtagctc caatcaaagt gacttccgct tgcgggtaac
     3901 gttcaaatgt ttttaagata cctaactgtg tatctgtatc gtctttttct gcaggggaac
     3961 gggtatactc tttggctttt gcaaatactt ccttctgctc ttcgttcgac aatgaatcaa
     4021 aatcacccac tgccagatct actgggtagc ctgcttgaag caggtacagc gcaccacggt
     4081 ctatcccgac ataggcatcg tatttggaca gcagagactg cggccagttt tgcggattac
     4141 ctccagcagc taataaaatt ttcatttagt ctaatgcctc ttttaatcgt tcgatttgtc
     4201 cgactggatt ttcggctcca tagatatatg atcctgcaac gaagacatcc gcccctgcat
     4261 ctttacagat ttttgctgtt tctggaacga ttccgccatc tacttcgatt gcataagtcc
     4321 aattgttctc tctacgtaga tcagcgagtt ccgtaatttt ttctactgtt tcttcaataa
     4381 agctttgtcc gccgaaccca ggattgacag tcatgactag tacttgatca gccaaaggca
     4441 aaacatgttt gatcatggag actggtgttc ctggattgat aactactcca gctttaactc
     4501 ctaagctttt tgtcatttga agtgcccgat gaatatgagg agttgcttcg acgtggacag
     4561 taatgatatc tgctccagct tttgcaaatg ctggaatgta gttttctgga tcaacgatca
     4621 tcagatggac gtctaacggt aatttagtaa caggacggat tgcctgaaca atatttggcc
     4681 ctaatgtgat atttgggaca aatcggccat ccattacatc tacgtggatg taatctgcgc
     4741 ctcctttttc cactaactca atgtctcttt gtaaattggc aaaatctgca ctcaaaatgg
     4801 atggtgctat cttcattctt gtttcctcct attttcgttt accttctttt tttgcataca
     4861 ttggttttct tttttcgatt tcttgtaaga attgcaaata attgtcgtaa cgtgtctgtg
     4921 cgataccacc tgtttctact ctatgtttta cttcgcagcc aggttcttta gcatgcatgc
     4981 actctctaaa tttgcaatgg acagaagctt ctacaaattc aggaaattgt ctcggcagtt
     5041 ctcctgcttc aattgtcaaa aagtcaatcg agctaaatcc tggtgtatct gctactaacc
     5101 cgccatagat aggaatcagt tccacatgac gtgttgtatg ccgtcctctt ccaagtgcat
     5161 cagaaatctc tcctgttgct aaattcagtt ccggtgatat ttgattcagt agcgtcgatt
     5221 ttcctgctcc agactgaccc ataaagaccg atagccgttc tgggaagtaa cggatcagtt
     5281 ctgtcgtatc acccgtatga ttgggtacaa tcaccggata gcctatttta gtataggttt
     5341 gcttgatctc ttcgattttt tctgggtcgt ctgacaagtc ggtctttgtc aaaaaaataa
     5401 tcggttcgat cgactcgtat tccaaggtaa ctagaaagcg gtcaagtaga ttataagaaa
     5461 atgcaggctc tacgatactt gtgacaatga ttccaagatc aacatttgtg actggtgggc
     5521 gaactaattg attttttctc ggcaagattt ccagaagata gccgtctgtt tgattttcac
     5581 tctcaaagat cacctgatct ccgacaagcg gcgtgatttt ccgattccgg aaatttcctc
     5641 ttcctcttgt ttgataggtc tctccgtttg cgtatacata gtaaaaaccg ctgattgctt
     5701 ttctgatttg tcctttgata agtgccacct ccgtttttgt ttattttacc atacgaagag
     5761 gaaagtaggt gttattccat gcaaacttct caaagtatat ggcttgaaaa ttgatcaaaa
     5821 gagatagaaa cacaaaaaag agaatgcaac aaatcaatgt cgcattccct tctgatatat
     5881 tttattcagc acttattttg aactttctga ggtgctagct gatgaacttg tggagctgga
     5941 agattcttct tttgaagagt tgcttgtgct actggagcta cttgagctgc tagaactgtc
     6001 tgaagagtct ttttcagaac tagaagacgc tggatctggt cctttagagt aaattacgtt
     6061 cacaaccatt cctttggtaa ttgtcgttcc tgcccctggt actgtacgaa tcactttatc
     6121 tttatccacc gtgtttgaat attcggaagt ctcatgcccg atatattctg caccgacact
     6181 agctaaataa ctttgtgctt cgctttttgt atagccggag atatcattca atttaggctc
     6241 tgttccttgg cttacgacaa aattgatgta agtttcttct gctacaactt cacccgaggc
     6301 tggatcctga ctgatgatcg aacctgatgg tacagtgtcg ctatagtcat aagtagcact
     6361 aatattattg atattgaatc cttgtgtaac aagccaatta cgaacagttt cgtagttttc
     6421 tcctacatga ctactcaaag tcactgtttc tggtcctttg ctgactgtga aagtaatcgt
     6481 gtctgtttta ggatcgactt ctgtatcttc tgatggagat tgcgaaatga tcattccgct
     6541 ttctacctct gaactgaatt cctcttcttt ttgaatcaag tcagcagaaa accctaattc
     6601 tttcaaactt gcttcggcat cttcataact tttaccactg taatcatcta atttgatttt
     6661 tttcgtgccg ctactaatat acagagtgac ttcgcgtttt tgcttgactg tcgttcccgc
     6721 tgccgggtct gtcttcacta cattaccttc ttcgattttg tcatctgcaa tttcttctgt
     6781 ttttgttgct acttctaatt tcgcttttgc gagtgtttct ctagcagaag cttcagaaag
     6841 atcagaaaca tcaggaacaa caacttctcc tcgcccgcca gaagcaaaga atacagctgt
     6901 tccgattcct aaaacagcaa gaatagctaa taggatcagc caccattttt tctgtccttt
     6961 ctttttagga tcaggcagaa cttcttcctc ctctggtaag gtttcgtttt ctggcttgtc
     7021 ttttggcaga ggcatagatt tgaatgaatc aggcattggt gcatcttccg taaggggtgt
     7081 gataactttt gtttcattat ccatcacatg aggctgccat ttttcttcgt ttgcacgagc
     7141 tggtgataat actgtagaaa gatcatcaga catttcctcg gctgacttat aacggtctgc
     7201 aggttcttta gccgttgccc gtaaaatcac gttttccagt gcttgtggca catttggatc
     7261 aagtgctttt atagatggaa gatcatcttg aaaatgtttt aaagcaattg ttacagcaga
     7321 ttcaccatca aacggtacgc tacccgtcaa catctcataa aggatgatcc ctaaagcata
     7381 aatatctgac tgcttcgttg ccatacttcc tctggcctgc tcaggggaca gataatgaac
     7441 agatcctagc atcgtattcg tctgcgtgat cgatgtttca gataaagcga tagcaatccc
     7501 gaaatccgtg atttttacaa caccttcatt atcgatcagt acgttttggg gtttcaaatc
     7561 tcggtggatg atttgatggt tatgagctaa agaaatagca gaaaggatct gctgcataat
     7621 atttactgct gtttcataag gaacaggata atgtgtttgg atatatcgtt tcaagtccat
     7681 tcctttgacg tattccatca ccagatactg catgttgtct tcttcaccaa catcgtaaac
     7741 actaacaatg tttggatgaa cgagttctgt tgctgctaaa gcttctctct gaaatcgtcg
     7801 aatcgcagtc tggtcatttt gaaagtcaaa tcgtaagact ttaactgcta catcacgatc
     7861 caagattaaa tcgtgcgcca aaaatacatt tgccatacca ccgctaccga tatttccagt
     7921 gatttgataa cggccattta attttttgcc caactcgatc atacactgtc ctccttatat
     7981 tcgataagaa gaacggtgat attatccgct ccgcctgctt cattggcacg atcaatcagc
     8041 tcagttactt tatgagacaa tgggccttct tgattgatga tcgttcctat ttcttcttca
     8101 gacagcatgt tcgtcaagcc atctgaacat aacagcagtc gatcttttag ttgccaaaga
     8161 tgagtagaaa catctacttc gactgttcca ggcatgccga cagaacgtgt taaaatattt
     8221 ttccttggat ggttcactgc catttcttcg ctgatttctc ctgatttgac tagttcattg
     8281 actaaagaat gatcctccgt caattgcatg attttatctt cacgaatcaa ataagctctg
     8341 ctatccccaa cattggcaat cgtgaaccag tcatctgaca gagcggccgc tacgattgtt
     8401 gttcccatac cgttatattc cggctgttct tgtccacgct gataaatcag catattttct
     8461 tcttgaatcg attgaatgaa ccattgggcg attttttcat cgtcaacagc ttcttgtttt
     8521 tcccaagctt cgcctagatt cgttaccgcc atctgactgg caatgtctcc ggcacgatga
     8581 ccacccatcc cgtctgctaa tacagcaagc ttgagacctg aacggctggt aaatacactg
     8641 gcatagtctt gatttgtgtt acgtcgtctt cctacatctg attgatattc aatctgcatt
     8701 ttttcacctc attacttttt gcgcaaacaa caaatgaaaa atccatctgt gtaaaactga
     8761 tgcggataca aagtcaacat tttctcttcg attgatgatt gtagcatagg attggtcaaa
     8821 acatcaatcc tctcaaaatc agggtgtctt tccaagaaag cagaaacaac gtcttggttt
     8881 tcttctttta agatcgtaca agtgctataa gtcagaatac cggaagactt taaagttggt
     8941 gcgcagcttt ctaaaatttc caattggatt tttggtaaat tcgctaattc atccggctgt
     9001 tttttataac gaatatctgg ttttcgccga agcagcccaa gacctgaaca aggcgcgtct
     9061 actaaaatcc gatcaaactg ctctgcttcg aactgtttac tgacttctct agcatccatc
     9121 ttttgagcgt agacagcaga agagacacct agacgtgctg cattatcttc aatgagtttg
     9181 actttgtgtt cgtgtacatc taaagcgacc acttctccac cgcatgacgg atctaaaaaa
     9241 gaagcaatat gcgtcgtctt acctccgggt gctgcacagg catctaagac atgatgttca
     9301 ggctcgattt gcatagacgg cgctacaagc atcgaacttt catcttggat cgtcatgacg
     9361 ccatcgtgga acaatgagct agaagctaaa tgcccttttt tcgctactat accgtatgga
     9421 ctgacttggc ttttttcagc tgtgatctct tcttcttgca aaacagtgat ggcctgatct
     9481 ctggaaagtc ggcgtgtatc tactcgggca ctagcatggc taggctgata tagcgataaa
     9541 ccgagctttt cagtttcttc gtatccgatt tgatccacca gtcgtttcgt catccataga
     9601 ggcaggctga ttttgacaga aagtctttcg ataggatcag caatgccttg cagatctggt
     9661 gctccttgac gctgataatt cctcaagaca ccatttacaa atttccctgt cccaggattt
     9721 ccttttactt ttgcgatttc gaccgcctcg tttaaaatag catgtgcagg gactttatct
     9781 aaaaattcta attggtatac agaaagataa agaagcatct tgacccagtc atccactttt
     9841 tttgctttag cgatcaatgg tgcaacgtaa aattccaata gtcgctgatg gctaatcgta
     9901 ccatagatca attctgtaaa taaacccaca tctttttgat ttagctcgct ttgtttgatc
     9961 atctcattca acaaaagatt cgagtaggcg ccacccttgt tcactcgctc taatgtcagt
    10021 aatgccatat atcgtactga ttttttcatt tttttaggga tttttttcat tatttcacct
    10081 gctgaccaat ctgaacttgc tgaccgcttc cgttaaggaa atcatgtacg gcctgttttc
    10141 ctttaccagc tggctgtaat tcattgatgg ctaagacggt ctgtttaccg caagcaatcc
    10201 atagattttt tttgtctttc cggatgatcg taccaggtgc tttgtctgta gtttctgcca
    10261 atacttcaac cgataataat ttccagcgta tgccctcata ggtcgtgaat gcaattggcc
    10321 aaggacgcat tccgcgtact tggttgtcaa tttcttctgc agttttatgc caatcgattg
    10381 cttcttgttc ccgtgtgatg tttggcgaaa aagttgcttc tgactcgtct tgaggtactg
    10441 gtttttgtcc atccaaaata ttgggcaatg tttctagcaa tagctttctt cctaaaaggc
    10501 taagcttttc aaacatcgtc cctacatcat cttgtttagt gattggtatg ctttcttgcg
    10561 catagattcc gcctgcatcc atttttttga tcatttccat gatcgttaca ccagtttctt
    10621 tctccccatt catgatggca taatgaacag gcgcacctcc ccgatattta ggaagtaaag
    10681 aagcatggac attgattgcc cctaattttg gtacttgcaa taacttttca ggcaagaatt
    10741 ggccaaatgc cgcagtgacg atcacatcag gcaccaattc aatgatttct tccatttctg
    10801 gcgaaccgga gattttctct ggctgcagca cacggatacc gtgcttcaga gccgcttttt
    10861 ttaccggtgt cggcgtaatt actcgtttcc gcccaactgg acggtcgggc tgtgttacca
    10921 ctgcctggat ctcgtaacct gattctatca gcccttcaag aatcggcaca gaaaaagcag
    10981 gtgtgcccat aaatactagc ttagtcatca agatgttcct ccatatattc ttcaagttct
    11041 tcttccggga tctgctgtat cattttctca atgaacaaga taccgtctaa gtgatcgatt
    11101 tcatgctgga aggcacgggc taaatagcca aaagccgtca cttctatctc ttctccgtct
    11161 cgatcataat aacgcacggt cacttcatct gctcgcttga ccgttccata gacatgcgga
    11221 atactgagac aaccttctac gtcaaggctt tcaccttttg cttcgatgat ttctgggtta
    11281 atcagttcaa atttctcgcc ttcatctact tcgatcacag caattctctt gttttgtcct
    11341 acttgaggag cagcgatccc tattccgtca ttcgcaatca ttgtctcata taaattgtct
    11401 aataaagcga ttgtctcgtc cgtgattaca tcgattggct gagcagttct ttttaattta
    11461 tcattagggt ggatcaatat aggatagcgc ataatttggc cctttcttaa ataaagttta
    11521 atggttcgtt gtcaattgcc acaaaaagac cttgtcgctg ttctttctgg cttccattca
    11581 aaatttcatc caaaacatga ggaagattcg gttcatgttt atatttgatg attagttgat
    11641 aataatatcg gtttttcact ctcaaaatca tgctgggcgt tggccctaat agcaagcttt
    11701 gagggcttaa cacttctttt agctgattgg caatctgaaa gattttcttt gccgctactt
    11761 gttcttcagg atggctggcg gttattttca cagtaaagta ataaggagga tagcctccac
    11821 gatgccgtac atgcatttcc tgctgataaa actgctcata attttgtttt ttagccaatt
    11881 ctatggcgta atgttgagga ttgaatgttt ggatcacgac ttcgcctgct ttttccgctc
    11941 gccctgctcg tcctgaaact tgggtaagca attggaatgt atgctcacta gaacgaaaat
    12001 ccggtaaatt cagggaggta tcagcattca acaccccaac taatgtcact tctggaaagt
    12061 ccaaaccttt agcaatcatt tgtgtcccta ataatatatc tgcttctttt gcaccgaatt
    12121 tttgtaaaat ctgttcgtgt gccccttttc ttcgagtggt atctacatcc atacgcaaaa
    12181 tcctagcttg cggataaagt tctcggagtt cctcttctac tttttgagta cctgttccat
    12241 agtagcggat tttattgccg ccacaattag gacagcgatt gggaataggt tcttcatgtc
    12301 cgcaataatg gcagcgcatt gaacgtgtat ccatatgcag tgtaagagag atatcgcaat
    12361 taggacatgg aagaacaaat ccacagtccc tacacatcac aaaagacgaa tatcctcgac
    12421 gattgagcag taggacagtt tgttcttttt tgtctaatcg attttgtatt ttttcctgca
    12481 aattagcaga aaaagtagag gtccgatgat tctgaaactc ttcacgcata tctacgatct
    12541 caatagctgg aagctgtgca gctgctttgg ctcggtggtt taactgcaat aactgataaa
    12601 cgcctttttg agctcgtgct cttgattcta aagagggcgt cgcgcttcct agaactattg
    12661 ggcaatgatg atatttactg cgccaaattg ctagatctct cgcatgataa cgcggtgtct
    12721 catcttgttt gtaacttgat tcatgttctt catcgataat gatcagcccg atatttttca
    12781 gcggggcaaa aatagcagat cgtgcgccta ctactacttg cgcttcttcc cgctcaatct
    12841 tgcgccattc atcatatttt tcaccttggg aaagtccgct atgtaagacc gcaactgatt
    12901 ctccgaagcg tcctttgaat cgctcgacca tttgcggagt caatgcgatt tcaggcacta
    12961 acataatagc cgttttattt tctgacaaaa catcagcgat tgcctgaaga tatacttctg
    13021 ttttcccgct gccagtgatt ccctccagca aaaagacctg atctttttgc tgctgacctg
    13081 ctgtgatgat ttgttcgaca gcattttttt gttctgcatt cagttccaat gccgtcgttt
    13141 ggtcaaatac tcggtcttta taagggtcgc gataacgttc tgattctata aactccagcc
    13201 agcccttttc ttttccttga ttcaagacag cagtactgat tcctttattt ttgaaataag
    13261 caattggttc ttcttggctc agttcagata aacacaaaag aagttctttt tgtttcttgg
    13321 cattttgccg taattttgcc cattcttctt cgatttgctt ttcagttaac aaagaacgga
    13381 ccaaacgaat cgtttttact ttatttcgtg tatggacttc atatttgata tctactaatt
    13441 gctcttggcg ccagcgaatc aattgagaaa ggctgccttc ttcttctgct tccttccagg
    13501 aaatcacttc tcgctgatca aacaatacct gaacttctgg tgtgtctgcc aaaggataga
    13561 tcaatttatc atattctgct ttcatcacac tcggaagcat cgtttgtaga caagtgattt
    13621 tgaatgcgta agtaattttt ttcatataat cagctaatga aagtaattct tcattgacca
    13681 ccggagcaag gtctagcaaa cgaataactg ccttcaaaga tggattagct gattcttctg
    13741 atttttggat agccataaca aatccttgga catgacgatt gccattacca aaagggactt
    13801 ctactcgcat cccgacttgc aaagctgttt ccatttcaga gggaaccaaa taagtgaatg
    13861 gctgatcggt ctgcatagta ggtacgtcca caatgacttc agctgttaat gtcatggttc
    13921 gtttcctcct ttttttgtct tttcttatca agcgacaaaa agttccttgc ctattttact
    13981 agagatcgat ggatttgtct tatcttctgc caaatcctaa caaaaaatta acagcagata
    14041 agaaaaaaaa gaactgtgtc aaaaagttca gacacagcct tttctaccag acaataagtg
    14101 gcgaatgttt gaaaaggaaa cagctgggtt tgcttttctt tttagatgtt cgctctattt
    14161 ttctttatta cattttttgc tcttcacgga tacgtgattc caattcgtgt tgttcacgct
    14221 ctttttgtgc ttgacgttcc tcttcttcca tcttcaaacg ttcacgtttt aattcaggat
    14281 gtggatcatt gactacattt cctgcatcga tttcttctaa agcttgaccg acgcttttta
    14341 ctgaatcaaa agaatctaga gtaggctgtg cacttgcatc caattcatgc gcgcgtttac
    14401 ttgctaaaat cactagagaa tattttgaat ttacacgatc taatagtgag tcaattgaag
    14461 gttttaacat cataagacta catctccttt aacattttaa tatatttacc gattacgcga
    14521 tctactcgaa aatgttcact ggcaataatg tttttgattc gttcaactgc tagcggcact
    14581 tcatcattta caacagcata gtcgtaaaga gccatcattt cgatttcttc ttttgcaact
    14641 ttcattcgtt tttcgattac ttcatctgca tctgttcctc ggcctacgat ccgtgatttt
    14701 aattccgcta aatctggcgg agtcaaaaag atgaatacac catcgggcac tttttctttg
    14761 acttgttttg ctccttggac ttcaatctcc aagaaaacat cttttccttc atccaatgtc
    14821 ttgttgacat atgacaaagg agttccataa taattcccga catattctgc gtattccagc
    14881 atctctccag cttcgatcat tgattcaaac tcttctttgc ttcggaagaa ataatcaacg
    14941 ccttcgacct ccccttcacg cattttacgg gtagtcattg agatcgaata ctgaaaatca
    15001 ttatcctcac tctcaaaaat tgcttttcgt actgtgcctt ttcctacacc ggatggacca
    15061 gacaacacga ttaataatcc acgctctgac atgacgacgt cctttcaact agtctttttg
    15121 tacttatcgt ttattttgca ctttttttct gcaactttca aggttatctt taattatagc
    15181 tagaaattgg taaaaaagct accctgtacc aagaggtcgt aaaaaagctg tcctgcatca
    15241 agtaataaga acagccaaac ataaacagct tctcatgttt ttcgtttggt tggggttatt
    15301 atcgcagatg caagcttttg aacgccgtgt ataagaggtc gtgaagaagc cgattagttt
    15361 cgagtatgaa gaaaaatttt gtgaaaatag tttctcacat ttttgcataa atttggctta
    15421 ataccgagaa aatggttttt gaacatcact tattcaaaat aaaaaaagat tgtgacacga
    15481 attttgtcac aacctttaaa tgtattccta gagcaaagaa gctaagcgaa gaaatccaat
    15541 tctgtttatt tattgtattt ctttgttcag catccttgct aaaagcccat attggacaaa
    15601 gtattcacta cacccatgat ctcatctttt cgagaacgtg ctttttctgt cggatcttct
    15661 acctcttctg tgtcaattcg aacaataaat ttccgattca tcgtcacgta agcaccgtcg
    15721 ttgatcaaaa taaacagttc gttctcatta tagtctgcac aggcatcttt ccatacgtca
    15781 aagccgtttt tttcagaagg gatatcccgt ttaaccacag tttttccact taattcgtgg
    15841 aacgttacgt cataatattt cattatctct acctctttcg tcattcaata ttttgtactt
    15901 gttctctgat tttttctaag atcgtcttag actggacgac ttgattttta atttcgattg
    15961 catttgattt ggaaccgatc gtgttgattt cccgattcat ttcttggatc aggaaatcta
    16021 attcccgacc tacaggacat tgtgcttcga taagttcatc tagtttttgt acatggatcg
    16081 ccagacgatc cagttcctcg tgaatatctc cgcgttccaa caagatggct agctccgtca
    16141 acaaccgttg ctcatcgact ttcgctccaa gaaattcctc cagctttttt tgatacctta
    16201 tttgaaattc cttttcatag atttctacaa aactatcgag tttttttagt acttctttga
    16261 attcttcagt attctttttc aacactaaag ctaaagcttg accttctttt tctcgactcg
    16321 cctcaataga atcaagtgcg cttttcaatg tatctagagc taactgtgcc aattcttcca
    16381 tgctctcttc cggtttttct tctaccgtta cgaagttttc gtccagcacg aagcgttccg
    16441 ccatccgact aattgacact tgattttgac taccgtatcg ttgttgaatt ccgtctgtca
    16501 attgagtgat aagtgaatca atcaattccc agttgatgac gatctgtttt tcgtttttgc
    16561 caatataagc tagattcaca aatacttcga ttcgaccgcg gcttaactgc tgtttaataa
    16621 gctgacgaaa atcgttttca acaaagttca ggattttagg acttcgtatt tgaacatcta
    16681 gaaaacgctg gttgacactt tttatttcaa tatctaactg atacgctttt gtttcgcgga
    16741 tggctttgcc aaaaccagtc atacttttca ttcgacaggg tccttcctca ttacatattt
    16801 tctattgctt tttgcagttg ttcaaactct tcgttcgtca atgtcaggcc tttccccatt
    16861 ttttcatggt ctggcgccca atctcgaaga tcgaattttg gcgggcgacc attccagctg
    16921 actaggttca gttctttacg ccaccccttg ttgttttctg acaatatagc gatttcttct
    16981 acgatttcat atgaaaattc ttgtgccatg gttgttcctc cttgagataa tataaatata
    17041 ggatcagtaa tgtgtcaact ggttgcttaa gcaattcatg ctgcaaacga tccttgcgtt
    17101 ttccttgtag cagataatca aaataaacag ttttcgtgcg ctcattttgt attttttgct
    17161 ggtattttcg ataccttcgc cattcttcag tacttaattg accaatttgt gccaataaat
    17221 catccggcat actttttata tcttgttggc agatcatttg aatcatcatg tctacttgat
    17281 agatttcatt gggcagtaaa aaatgttttt ttgctaaaag ttctagcatc gccaatccgc
    17341 atgtctctgg tacgaccctg ttcttatata aattacgcag tttttgccaa aattttatgc
    17401 ttttgaaaaa agataattct aactgaattt cttttgttaa gtaagacaaa aatcctcgcc
    17461 agtatgttct tgcaggttta acagaatgaa atgtatacca gttcccatag taatagccta
    17521 atcgtttttt catttccaga tagcctaatt gtaaatttaa ttggttcctt tggctatcaa
    17581 aaagcagaaa gctgcctagt gtccacaatg tttgacattt ccaatggata aaaatgtctg
    17641 gcatacgaat ttttttcgct gggcctggcc cttgcacatc tacgacaaat gcctctgttg
    17701 ctcctttgtt aatcgcaata tcaataggta ttttgttgcg atacccaccg tctgcatatt
    17761 tattttttcc gatctttcga taagccatag cgggataaaa tgaagcagag gcgagtatcc
    17821 aatctggtat ttcttctgtt tttgtctgtt ggatatcagt cactacttcc gctagttctg
    17881 gcagtttagt agacaccgta tataaacgta ttttgggatt atgtttgagt aaggacaaat
    17941 ccaaattgtc tttgaccatc ttttccaatg gagcagaaga aaccccgcct tcaattagtg
    18001 ctgttttcgt catttggcgt ctttctcgat agaagcgttc acgaaggtct tcaactgcag
    18061 ccatttctgg taacgcaagc acttgtcttg tcgacagctt tttccataaa ttcacagctt
    18121 ttttcatatc tccttgtaaa atcaatgcgc cgttcaacgc acctaccgag gtacctgtga
    18181 taatctcaaa gttgatatca tgttccttca gcgcctgcca cacaccaatc tgataagcac
    18241 cgtgcgctcc tcctccacct aaaacaagtg ctgtatgata gaccagcttt ttatttaatt
    18301 gtgtggttcc ttgatagccg tttttatgaa aaatacgcaa ccattcttct gatagaggag
    18361 attgaaataa aaaacaacag ttttctttaa taaaaaaacg agcgcaagct tccatatacc
    18421 gaaaaaattt tttccattca aaagtttcac cttcaatcaa cagattatta aaaataacat
    18481 ctcctctact tttagcaata gtaaaataga cggtatattt tttcgatact gctttaaaag
    18541 cacattgctt ttttgcagaa cgccaaaaag cgtctctact ttcttgatga ctttgaaaaa
    18601 atggaagggg ttgttttgct tgctggatag gcgaatacag cgacattttc tgtgaaaagt
    18661 aaactttttt tatctccatc gcgctctcct ttttcttcaa gtataccagt acgtcataaa
    18721 aaggtcatga gtctcgtatt cgactttgta gattccataa gaaaacaatt cataaaacct
    18781 cctttaaata aaaaaataca aaaatcacaa aaattaattg aaaatgagag tgttttcaat
    18841 gaaaacgtga ttttgtaatg gaattttaaa ataaaagaaa caagcttttt taatgcaaat
    18901 gttgtaaaat aaaagagatt aagaggaaat aagaggagga acatgatgac tgagtcagtt
    18961 tatgtacata ttgataccac aagtaatgct gttttaacaa aagggctgac tgctactgac
    19021 tttgcaaaca gtatcgtgca ttttcctcaa aacttgctct tgcttgatcc atctgcttct
    19081 gctggagaat atgaatccca tacagggctg aaagtaatcc gtggtacaga aaatattcag
    19141 cgtttctttt cctcttctcg taacaggcgt ggattcgatg aattgaagtg gatcgacttt
    19201 actgatttaa cgatgctgaa agaattgaca cctttagaaa tttcagaatt attatatttt
    19261 ggtcatatga aaacacatct tcattcgccg tttttttata aactacagaa taattttgta
    19321 tacttcgatc ttaatgatca gctgaatcgg atttattatc gctatttaga tgaattttat
    19381 aggatttttg ctagtaaatt aagttcgatc ttgtcagata agatcaatgt aaaaaaatct
    19441 tttttcaaaa aagaaacacc tgttgaaaaa atcagtgtgg aattattgaa agaaatgcgt
    19501 aatttgatgc aggaagggat cattttttct tttgatcaag ttggtttgat cgatggtaaa
    19561 tatcgtatcc caatctatgt gattgaagat tatatttgga aattaaaagg cacccggtat
    19621 cgagaagagg aaatcatcgc tactttaatc tactctgtca ccgatcgaca ctggcaattg
    19681 gtagaagatg ccgatctttc ttatctgcca ttgaagtaga tctatttctt atcaaaaaat
    19741 aaaaaggaaa caagaaacta ggacatgatt ctgtctcaag tttcttgctt cttttttatt
    19801 tacagctcct gtcgtttatc tggcacaaaa atcgaaggag tcgtttgttg gatccctaat
    19861 aaaacgatga tcgttacaat agctgtactc aacccgctgg caccattcat tacgaaagaa
    19921 aagagccaag gattcatccc ccatagtgca tacgctcccc agaaaaccac tcctgcaata
    19981 aaatgccaaa aatatctgga aagtgcgcca atcaatgcac caaacacgat gctttttcta
    20041 atttgtttcg tattatcgtc tacatagcct ttttggacat ttgaagccat gagccctgct
    20101 gcgcctgcaa aagtataggc aatcgggtac tcaatcaaca cttgaacgac acttaaataa
    20161 taaacttgtc ccaacggaaa atgtaggatg ccccaaatga aacctgccag catccctgcc
    20221 tttgtccctc ttcttaacgc aaaaagagtc aatggaatct gccctaatga aatagaaaaa
    20281 ctgctgccga tctggatcgg aatcaacgac aacaccatcg ctaacgctgc aacaatcgtt
    20341 ccttctaccc aaactctatt ttttcccata aaaaaacttc ctcctttgaa taaaaaagtc
    20401 acaaaggagg aagcagcttt cgaaaatagt caaacagctg tttattcaca attcctacgc
    20461 tcgcataacc gatacaggta cgaagggttt agaaatagat tcaatctcag cccaattgga
    20521 ctcccctttg tgagtttatt tattatttat tttttgtttc aaacaatgga ctaactggtt
    20581 tgttttcatg gatacgtttg attgcatctc ccattagtcc gccaacactt acttcatcga
    20641 ttttgtcgat tttacgatct tctggaagat agatcgagtc tgtaacaact agacgctcga
    20701 tagctgaatc ttctattctt tgtaaagcag ggccggataa tactggatgt gtacatgaag
    20761 catacacact agttgcacca gcttctttca aagcattggc agccaatgtg atcgtacctg
    20821 ctgtatcgat catatcatcg atcagtacac aagttttgcc tttgacttgt ccaatgatat
    20881 tcattacttc tgcaacattt gcttttggac ggcgtttgtc tatgatcgca atcggagatt
    20941 tcaaaaattc cgctaattta cgtgcccgtg tgacaccacc atggtctggt gaaacaacga
    21001 caacatcatc gcctttgatt cctttttcta agaaataatt ggcaataagt ggcgcaccca
    21061 tcaaatgatc gactggaata tcaaagaaac cttggatttg gactgcatga aggtccaaag
    21121 tcagcattct tgttgcacca gctttttgaa tcatgtttgc tactaatttc gctgtgattg
    21181 gttcccgtgc acgtgcttta cggtcttgac gcgcatagcc ataataaggc atgactacgt
    21241 tgattgtttt tgcacttgct cgtttcagtg cgtcaatcat gatcagcaac tccatcaagt
    21301 tatcattgac tgggctgctc gttgattgaa taacatagac atgcgatcca cgaatacttt
    21361 cttcaatgtt tacttgaatt tctccatcac taaattgagt aacagatgat tttcctaact
    21421 cgacgccgac tgctgctgca attttctctg ctaatgggcg attggaattt aacgcaaaaa
    21481 tttttaatct tgggtcaaag taatgttttg acatggggac ctccactttc tttttcacaa
    21541 ctctagaatt ctacctaata aatagtagtc atatttttta aataaatcaa gtgattttgc
    21601 aaaagtctta gtttagataa gggagttttt ttgcatatcc ttctttattg acttgtcttg
    21661 ctctcgcaat tgccagcgca tattctggaa tattgtctgt aattgttgac ccggcagcaa
    21721 tcgaactgtt tttagctacc tcgacaggtc cgatgatatt cgtgctagaa ccaataaagc
    21781 tgtggtcgcc gacagtcgta tgatgtttgt tctttccatc atagttgacg aagacaacac
    21841 cacatccgac attgatttct tctcccagcg tagcatcacc aacataagtc aagtgaccga
    21901 ctttagttcc tttgccgatt tgagcatttt tcacttccac aaagtttccg atatggacgt
    21961 tttcgccaat ttcagctttt ggtcgtaaat gtgcataagg tcccacatct gcatgtttct
    22021 tgacatggct gctttcaatg actgagtttt caatgacgac gtgatcctca atcctactgt
    22081 ctacgatttt tgaatgtgac ccgatgacac aatcagaacc aatgactgta tttccttgga
    22141 tttgtacacc tgcttcgatc aatgtgtctg gaccgatttc tacgcctgca tcgatgtatg
    22201 tcgttgctga atcgacaaag cttacgccat tgaccatatg catctggtta attcgctttc
    22261 tcatgatctc atttgcttta gctaacgcga tccgatcatt gactcccatt gattcttcaa
    22321 aatcatccgt ttgataagca gcaactgtat ggccttcttc ttttaagatt tcgatgatat
    22381 ctgttagata gtattcacct tgagcattgt ttgttccaac tttgcttaac gcatcgaaca
    22441 aagcttcgtt gtcaaaacag taagttcctg tattgatttc ttgtacaaga gcttcttctg
    22501 gtgttgcatc tttttgttcc acgatttttt ctacgatccc aatatggtca cggatgattc
    22561 gtccatatcc tgttgggttt tctgcttgtg ccgtcaagat cgttgcactt gcattttttc
    22621 cttggtgata ttcaaaaaga ttgttcagcg tttcagtagt caataatggt gtatcgccac
    22681 tgataactaa cgttgttcct ttttttcctt ccaagaaaga agcagcttgc aaaacagcat
    22741 gtcctgttcc caattgttct gcttgtaacg catatttact gcgttctcct agttgtgctt
    22801 taacttgttc tgctccgtgt ccaacgatcg taatcacttc atctggtttc gtttcactga
    22861 cccgattaat gatatgttct accataggtt taccagaaac gggatgtaaa actttatata
    22921 attttgactt catgcgggta cctttccctg ccgccaaaat aattgcataa cgtgcgtcca
    22981 actgcgtcac tcctaaatat agtttgctga tttcagttta tcccagtata cccatttttt
    23041 caacaaaaca agtgattttc ttttatccca gcgttttctg gataaaaaaa tagatagacc
    23101 ataacgggcc tatctatttg gtaaccggtg ctctcctgcc aactcctcgg tcacaagtca
    23161 cactactcat aaaacatgaa aagctgtttt ttgaaatcgt ttcttgtgac ttagagttaa
    23221 acggtaggct tacaacctct tcattaaacg tttttcaatc agctgacctt cggtattagc
    23281 tcaataaacg gcaaaatatg aggagcaatt tttccgttta ttgagctaat actcaggaca
    23341 ggacgctgat ttcacaacct ctagataaac ggtgttcaaa agccagtttc tcggtattaa
    23401 gccaaattta tgaaaaaatg tggaaagcca ttttcacaaa atttttctta ctactcgaaa
    23461 ctaagcggct ttttcacaac ctcttattct gaaaaataat ttcctggcac aaccgtaata
    23521 tttttcgttt gagtatctac atctttgaca taaagcagtg acgtataatc atcgatggcg
    23581 cgatgaccgt tgaattttga ttcggcaaat acagtgatgc caacaagttc tgattcgaat
    23641 tcttcaatca agctcttcat tccgttgact gttccgccgc ctttcatgaa atcatccact
    23701 acaaggacac gtgaaccacg tttcaaactt ctcttagaca gttccatctt ttcaattctt
    23761 tcagaagatc cagacacata gttgacactt actgttgatc cttctgttat ttttgagtcc
    23821 cggcgtacaa taacgaatgg cgcattcaga taataggaca cagcttgggc aataggtacg
    23881 ccttttgtgg ctacagtcat tacagcatcg attttttcac caaggtattt agaagcaatg
    23941 attcttccaa cttgacgcag cagttcaggt tctcctaaca aatcagagag ataaacatat
    24001 cctcctggaa gcagacggtc ttgttctgat agacgttctg ctagagattc aatatatttt
    24061 ttcgcctctt catatgaaat ttcagggatg aacaatacac cacctgcagc acccgggatt
    24121 gtttccaagg ttccataccc tcgttcttta aatgttttct tgatgattcc caaatcttca
    24181 ctgatagaag atttagcaga ttcataacgt tcggcaaagc ttgttaagga aatcaattca
    24241 tgcgggtgat tcaataaata ctgtgtcatg tcaactagac gttcactgcg acgtactttc
    24301 acctattcca actcctttta agaaatttta tcgttttaca ttatataagt ttttcttcaa
    24361 aaattcgagc attttatgaa aaaataagaa aatagttcgg tatttattaa aataaaaaga
    24421 agcctataaa aaatttttcg tttttataag gcttcttttc tttataattt agttattttt
    24481 tcttctgaag gcaaccaata ttcgtttgat gatatttaca atcaggaaca aagcaataaa
    24541 gactaatgtg attgttgctc ctggaggtgt atctagataa aacgagctag ttagtcctga
    24601 cagcatccct agaaatccta cacaaacact gatagcaatc acgacattga agcttttgcc
    24661 taatcgcata ccgattgctg cgggaagaac catgattgcg gatatcaaaa gtgcaccggc
    24721 aatcgggatc atcacagcaa tagccacacc agtaatcaca ttgaacatca tcgacatcaa
    24781 atgaacgggt agcccatcca cgtgggcggt atcttcatca aaagtcagaa catacatcgg
    24841 ccgtttgaat aaaaagaaca atataccgat ggcaacgaat aagattccta aaaagatgac
    24901 ttgctcccat gtaatggtca cgatcgaacc aaacagatag gattggatac ttgttgctga
    24961 attccctccg ctcaaattca tcaatactaa cgctagcgcc aaaccaccag ccattaaaat
    25021 ggcaatggag atttctgaat aggttcggta gatcgttcgc agatactcca atatcacagc
    25081 tgctaataca acgactagca tggtcgtaag tgtaggatta aggttcaaaa agaatcctaa
    25141 cgctacccct gctaaagaga catgagaaag cgtatcagcc atcagcgatt gtcttctgat
    25201 cactaaaaac acacctagca tcggggcgat tccggcaatg aagatagcgg caagaaacgc
    25261 tcttctcatg aattcatatg aaagcaacgc cattgagaat cctcctttct caccagtcgg
    25321 atctgtctgt ccgcatactg tttgatatct tcatgatcat gggtgatcat tagaactgat
    25381 ttcccatgga catgcgcact atgtcggaga agacgataaa attcatttct ggattcttca
    25441 tccatacctg ttgttggttc atcaagaaca aataagtcag ggtcagtagc aaaaatacgc
    25501 gctaaactga ttcgctgctt ttgtccgcca gacaattcac ctattcgccg atttcgcata
    25561 tcccacatcc ctacagcttc taaagcacgc tgtacatgtt gatgatcttt ttcagagaga
    25621 cgcttgaacc agcggcctct tggatatctt cctgaacgga ctaattcgat caccgtacta
    25681 ggaaaacctg cattgaaaga tgcaacttgt tgaggaatat aaccgatact taattttttt
    25741 ccttcttgat ttttctcagc aatctttatc attccttttg aaggtttcag taatcctaac
    25801 gatgctttca ccaaggttga cttggctgct ccattttctc cagttaaaat gacaaattca
    25861 cccgcatcta catgataaga gacatcttct agtacaggtt catcatcata gtaaaaggtc
    25921 aaatcagata tttcgatata acgcacctta caacctccat tctaaaactt aattgatact
    25981 ttcttttaaa gctttcagat ttttttccat gacagaaata taattttcgc ctgctttgat
    26041 ttgttcatta gtcaagcttt ctaaaggatt caacacttct agcttcactc ctgtttcatt
    26101 cgacaatgtc tcagctacct tcgaagaggc attttcttca aaataaatca ctttgatgtt
    26161 gtttttcttc acaaattctt ttagctcagc taagcgactt ggtgtgggtt cctgatctgg
    26221 tgaaacgccc gaaatcgcta cttgttttaa gtcgtattga ttcgctaaat aagcaaaagc
    26281 agcatgctga gtgacaaatg tgcggtttgt tgcatctttt agctcatcgg agtatttttg
    26341 atccagtgct tcaagctttt tgatataagc atcggcattc ttcgtaaaag tttccttttt
    26401 atctggatat ttttcgccta attcttcagc aatcgttctg acttctttta tagctaatac
    26461 cggatcgagc caaacatgtg gatcgtattc atgtgaatga ccatgccctt cttctgattc
    26521 ttcatgttcg tctgcttctt gcgattctaa acggtcgatt cctttagctg cttcgatcat
    26581 caaggtctgt ttgctatcca ctgaatcctt taacgaatcc acaaaaaatt ccatgtctga
    26641 actattatac acgaaaacat ctgcatcgga aatttctgct ctttcttttg cacttggttc
    26701 gaaatcatgc ggctccgttc cagcagggat caaaagtttc acgtttcctt catcccctac
    26761 gatctctttt gtaaaatcat acatcggata aaaagtcgta acgatcgtca aatcctcttt
    26821 tttgtctgct tccttgttcg tatttccaca tgctgcaaac agtaaagcac ctatcattac
    26881 cgtagctgtc aaaagatatt ttttcattta tatgctcctt tcaatgaaca aatcggaatt
    26941 ttacgattta ttaacgtgaa ctaatgtatc aaaataaaaa aacacagtca atagaaatcc
    27001 atcaatttct gatttaagca tgtatattct tgattaaatc gtaaaatgga tgagttgctg
    27061 ctcatatcta gccatttcac tacaataaaa aactgaagaa taccatttta ggattcctca
    27121 gtttttcatc acctatttta cttcttatat tttattgcag ggttcgtact aaataaactt
    27181 cctcacaaaa accttttaac ccattatata ctctctgcgc tcttgaataa tgttgacaca
    27241 aggcaaaaac ggttggtccg cttccactca tcaatgcagc atctgctccg tacttgatca
    27301 tacgctcttt gatttgatgg atgactggat gccgctttcc cgttatatct tctaaactgt
    27361 ttcccatttt tttgatcatc tgctgataat cttgctctcg gatggcttca gcaagtccgt
    27421 ggatgtcttg atgttggatg gcttgcgtat ctacttctct aaacactgta cgtgtagaga
    27481 cactcattct tggtttgacc aacacaaccc agcactgtgg catagaaggt aatacttgga
    27541 tttcttcccc tttgccaccg acaaatgccg tacttccgta gacacagtaa ggaacatctg
    27601 taccgacttc caaaccgatt tctgccagtt cttctaacga acagcccaga ttccataatt
    27661 ggttgatccc tcttagagca gctgcacagt ctgtacttcc tccgcctaat ccagctgcta
    27721 ctggaatatt cttcttaaca aagatcttga caccggtttt gatattgtat ttatttttta
    27781 gcaataaagc agcttgataa acattgttcc gttcatctac cggtaaaaat gctttatttg
    27841 tttctacaat gattttgtct tctgttattt cttcaaaaaa tagatgatct gccaagtcta
    27901 cgctcgacat caccatttcc aactcatgat acccatcttg ccgtctatat aatacatcca
    27961 gtcctaaatt tatcttggct ggcgcttttt caatcaattc catcctctca cccattcgaa
    28021 aagtctaagt cgcaagtatt ctatcatagg gaaaaataaa agtatagctt atgtctgcct
    28081 agtggttgat tttgtcatcg tttcaacagc tttcacagaa ttttctcttc ataatccaaa
    28141 caagttctag tgattcttat aattctgtga gcacttcttc tgccaaactc ggaacatttc
    28201 ctttttcttt tcctatcaga ctgagtagct gcatggtagc aaatccgaaa ttacttcctt
    28261 tcctataagc aaaataacgt ccttcccaat gatcgacata ttttaatttc ttttcataag
    28321 cagactggaa ggaatagaaa aaattaccat aattatatac gatatgcatg actttttctt
    28381 gaaagaaaga aaaatctgtt tctccaacat tagctaatgg agctaagcct agattggctt
    28441 ccgagtagcc cattttctcg tatacaataa acagattggc taacagatag tcagctaatt
    28501 cctcagattc tttaggtaag acccgcaaca aatcatatcc cgcctgcttc ttcgtcatcg
    28561 gctgagcggt gatgaagcca acgacttttt gatctttacg gacaataccg acaccgctag
    28621 ataatacata tgatttatca aatcgtccca cagagaagta tttttctcgt gcaccttgaa
    28681 gccatttttg tgaaacttca tttacagctt gatgtaattc ttccggcagt tgatcgtacc
    28741 aagtaaaatg gtatccttct ttcatcaaac gttgatattc aagttgctga ctcagccgag
    28801 acaagtcttc tgttgctaga tcaacaatcc ctgcttcacc tactttcgtg aagtgaaaac
    28861 ccaattcatg aagcatcact acgtagcttt ctgatatctt ataaaacgca agctgatagc
    28921 ctaaacaatc tgcttgtttc ataaactgat tcgttgctgc ccgcatcatt ttaggattgc
    28981 caactggatc tcctaaaaca aagcatttgt ttcctttgat ctgataacca aaaacgactt
    29041 gatcttggtc gtcttcttgg taataaaaat aactgtaacc tgacaagcga agctgatggc
    29101 ttgtagctgt gcctccgtat gtttccaata aacggtcaaa acggtctttt tcgtactcac
    29161 tgccaagctt cgtagtatct tcagctaaat attgataaag cgccactaaa gctagcaatg
    29221 agatgcccaa accaataagc cctgagaacc acacatcatc tgaaggaaac aaaataaaat
    29281 gatttcctgt tattcgaccg actaaaaagc gtccgctgtg atagcctgca attgcgtaaa
    29341 cgataaacaa aaagccgaac aagctagcat caaagattat agctccccaa gaataaacga
    29401 attttgttcg ataaaattct tttctggata accacacagc taacagaatc aagacatata
    29461 ccaatatcaa ctgccaagat aacgtacgtg tgattgtatt taaaatacca aaagatagta
    29521 aagcaatcgt aggcagaaat gcctttttga cttttactga aagtgctcta gcaaggccga
    29581 gtaataaaaa gccaaccaat agattcactg tctgatctaa aaaattgaat gagaacggca
    29641 ataatacttg aaatagccga ctgattgcag acagattagt gattgttgat agaagtacca
    29701 tcatgatccc cgcaaaatag acagcactta cgagcacgat atgcgcaacc ttttggaaaa
    29761 acagtcgcgg aatgttatct aaaaatcgat tgattttgac tcctgtatgt gtgatgaaca
    29821 atagaagtcc cgtcataaaa ggtagaacat aataaaacaa ccgataatat aatagccaaa
    29881 ctactgctac atcttgccga acagacaatt gactcattcc taagatcatc aaaacgtcaa
    29941 aagtccccat tccacctggg accatcgtca gcattcctat caatgtagca atcatgaaca
    30001 taggatagat cgaaagcatc gaaatatcta ttctcatcaa cttgccgatg atcaaaaata
    30061 ccgcaagggc tcccgtccat tgtccaaagg atgccagtag cagaccaagc aagccttttg
    30121 gataaaactc gaagaacaat gtctttctct ttagataagc aaaaaagaaa atgactggtg
    30181 ctaaaaaact accgccgaaa agccagatcc aatagcttcg aaaaatactt tctgactgaa
    30241 taaaaaagac atcaataaat gtaagaaacg accatacaga caaaccagtc agcataaata
    30301 gtgcgacttt tgatacagtg gctaaaacca tttttctatt tgtctgcttt ccataaaaac
    30361 tagcccgcag acttgctcct accacaccac caaaaccggc gagattgttg atcgtgtttg
    30421 ttacccacgc agcaagaaga aactcttttc tttccatttt tggttttcct tgtttttcta
    30481 agactctgat cgtcacccaa tcatacaaca acatcggagt gacacccagc agtccagcca
    30541 gaatcatgag caggatcgtc atgcggcttt gttgtcccat cctttgccaa atatcttgcc
    30601 aactcatgcc attagcaata tttgcgactt gatttgccac aaagactaac acggagccga
    30661 aaaaaatcag ttttaaaatc agactatgtt cctttatcca ttgcagcatc aagtatcgtt
    30721 tatttttcac attttcccat tcctaacttg tatacttttg tgtcataccc caatgttatc
    30781 ttatttattt taatttttcc actttagacc tgttctttgc ttgcgatgat agaaaaagaa
    30841 agatatccta atgctaaagg agaggataac atgaaagtag gatttatcgg cacagggaat
    30901 atggcacaag ctattattga tggtttatta aaaaaaggaa cagtctcgaa agaagaggtg
    30961 ttcgtttcca gtggacattt tgaaaatgca caaaaatttg ctgctgaaac tggcgtccat
    31021 gcatgcaaaa gtaatcagga agttgcatca caatcagata tcgttctttt agccgtgaaa
    31081 ccaaaaatca ttgaaaaagt attggaagaa ctaagagaat atagccagaa agtcttattc
    31141 atttctattg cttcaggaaa atcattaata gatttagaaa cagcgcttga agatcctcag
    31201 gcctccatcg tacgtgtgat gccgaatgtc aatgtcagcg taggagcagg cgtttctgcg
    31261 atttgcaaaa atgaagctgc cacggaggaa cagcttcagc aagctacgat gatttttgaa
    31321 gcaatcggca gcgtctatcc tttagcagaa tcagatttca gtacattcat tggccttgct
    31381 ggcagctcac ccgcattcgt ttacatgctg atcgatgcga tgggacggac tggtgtactg
    31441 aatggtttgt ccaaacagac agccactgag attgctgcaa aagcaattat gggttcttgt
    31501 caaaaattcc tttcaagtga taaaaatcca tgggaacttg tagaccaagt ttcttctcct
    31561 ggcggtacaa ctgttgcagg tgtagttgcc ttagaagaag caggattcat cccagcaatc
    31621 atcaaaggaa tcaatacgac gatcgagaaa gatcttgaga tgttgaataa aaaataatga
    31681 tagcaatgtt ttttcgtgcc ttacaaaaaa agaagagctg tggcttttgc cgcggctctt
    31741 cttttgtatt ttttattatt tagcataatc agttgcacga gtttcccgaa taacgattac
    31801 tttgatgtgt ccaggataat ccagttcttc ttcgattcgt ttacgaatat cacgtactaa
    31861 acgaacagcg tctaaatccg agatttcttc tggcttgacc attacccgaa cttcgcgtcc
    31921 agcttgaact gcgtaacttg attctacacc ggcgaagctg ttcgaaatat tttccagact
    31981 tcttaaacga cggatgtagt tttctaatga ttcacttcgc gcaccaggtc ttgctgctga
    32041 gagcgcatct gctgcagcta caaggacaga gataacagaa gtagcttcca catctccatg
    32101 atgagaagcg atcgcgttga ttactactgg gttttctttg tacttggcag ctaattccgc
    32161 accgatctca acgtgagatc cttcaatctc atggtcaagt gctttcccaa tatcatggag
    32221 caacccggca cgttttgcta attggatatc ttcgccaagc tctgctgcta agataccagc
    32281 taattttgca acctcgaccg aatgcttcaa gacgttttgt ccataactag ttctgaatcg
    32341 cagacgtccg atgatcttga ttagatctgg atgaagtgta tgagcaccga cttcaaacgc
    32401 tgcctcttcc ccgtattcgc gaatacgttc atccatttct ttacgagatt tctcgaccat
    32461 ttcttcgata cgcgctggat ggattcgtcc atcttgtatc agtttttcca aggtcatccg
    32521 tgcgatttct cttcggattg gatcgaatcc ggaaagaaca actgcttccg gtgtatcatc
    32581 gatgattaaa tcgataccag tcagcgtctc aagcgttcga atattacgac cttctcgtcc
    32641 aatgatacga cctttcattt catcattcgg aagggtaacg actgaaacag tcgtttctga
    32701 aacttgatct gctgcgcagc gttgaatcgc taatgataat aagtttttcg ctttgcgatc
    32761 tgcttcttct ttggcacgct gttcagattc ttttaccatt agagttaact catgattcag
    32821 ctcttcttca gtcgatttca tgatgacatc tcgtgcgtcc tcttttgata aaccagcaat
    32881 tttttctagt tctttttgtt gatcttcaat cattttttca acgtcttttt ctcgctcttc
    32941 aattaattgc tgttttgaac taagtgcttc ttctttactt tcaagtgagt tctcgcgttt
    33001 ttctaacgag tcatctttac gattcagcgt ttgttcgcgt tgaatcaaac gattctcttg
    33061 agattttaat tctaacttac tttctctcag ctcactttca acttcagaac ggtatttctg
    33121 attttcttcc ttagcttcta acaatgcttc tttttttaga gtttctgctt ctttttttgc
    33181 actatcgatg attccagcag ctgatgattg tgcaccagct atttctttct cgtgacgtga
    33241 ctttgcgacc ataaacccta aaccaagacc gacaattaaa ccgatgatag cgaggagaat
    33301 gttgaatacc ataaaatcca cctccatact atctttttca tttttatcat ctatacagct
    33361 tctatgataa attattctaa actcgtcaat atgcatcttc gcatatatgt gtacaaaaaa
    33421 catgcacaac tttattctaa tgtttgtacc ccgtagtgtc aactttaaat aaacaaagaa
    33481 acggctttct acttattcat taaacgatgg tttttaaaat aaatgacact agtttcatat
    33541 aggtaaacct ttaaattgag aaacttttct attaaattat tctttgtaga tgaacagata
    33601 tttttatttt atgtacccac tcgtataatt aaataactca cgatttcttt attcaataaa
    33661 aaaacggcag gtcaaggaga gtacctactc ttcagaccag ccgtttttgt ttatcataat
    33721 ttttattctt ctactaaagg caattcttct tgtccttttt catcttctgg gacagcgact
    33781 tcttcaccaa taccataagc tgctctaact agagcagaaa cttctgccat catttcagga
    33841 tgattagcca tatagatttt agcgttttcc cgtccttgac caatccgatc ttctttataa
    33901 gaataccaag caccgctttt atcaacaatg tctttttcta ctgccatgtc tagaagttct
    33961 ccttcttgtg agatccctaa accatacatg acatctactt cagcgatttt gaaaggaggt
    34021 gctactttgt tttttacgac tttgatcttt gtacggttac caacaatatc tgtaccttgt
    34081 ttcaattgtt ctgcacgacg tacttccaaa cggatcgttg cgtagaattt caatgcgcgt
    34141 cctccaggag tgatttctgg attcccaaac atcacgccta ctttttcacg gatttggttg
    34201 atgaaaatag cgattgtttt tgttttgttg atcgaaccag agagtttacg caatgcttga
    34261 gacatcaaac gtgcttgtaa cccgacatgt gagtcaccca tttcgccgtc gatttctgct
    34321 cgtggaacta aagcagcaac tgaatcgact actacgatat ctacagcccc acttgatact
    34381 aaagcatcag cgatctctag accttgttct cctgtgtcag gctgtgaaag aagtagttca
    34441 tcgatattca cacctaattt ttgtgcatat tgcggatcta acgcatgctc agcatcaatg
    34501 aaagcggccg ttccgccatt tttttgtact tctgcaatag cgtgtagtgc aactgttgtt
    34561 ttacctgaac tttcaggtcc atatacttcg atgatacgtc cacgggggta accgccgaca
    34621 cctaaagcga catctaacgc aagagagcca cttggaattg tagagatttg ttggtcgatt
    34681 ttttcgccca atttcataat agagccttta ccatagctct tttcaatttt tttcagtgca
    34741 gcatctaagg ctgctttacg atcatctgcc aatacgataa tcctccttat atttacaaat
    34801 cagagcaaca tttcattatc cttatcatat cttttttagt gaaaaaaagc aagaaaaaac
    34861 cgaacaaata ttcgcattta tcattttctt aaaagctgtt ttctcaaaag atcaaatcct
    34921 tgcataaccg cattatatct gatagcagca cgatctctgg caaagtgata tttttccgaa
    34981 accacgccgt ctttagatga aatagcgatc catactgttc ctgcagattg accttctaac
    35041 gtgtccggtc cagccactcc ggtgaaagct acactgtaat ctgttcttgc tatctcacgt
    35101 gcttgaatgg ccatctgctc cacacattct ttgctgactg ttccatattc tgctaatagc
    35161 tccggatcaa tctccagaaa accagctttt gtttggagcg agtaggtaac gaatccgcct
    35221 ggaaatactt cagatacacc agcaatgtcg cctaatgcgg cttggaaggc gccggctgtc
    35281 aaactttccg ctgctgttac agtctgcttg ttttctttca gtagttccac tacgactttt
    35341 gctagcgaat tatcatcgcc gtatccataa aaatattccc ctactcgttc ttggattttt
    35401 tcttctaatg ctaaaagagc ttgattccct gcctgaacat catttgtttt cactgtcagt
    35461 cgtaatgtca cttcatttgg tttggcatat ggtgcgatcg ttggattgat ctgtgtctca
    35521 atcagatctt ttagctcagt gaccagtcgc gattcaccaa tcccataaaa gcgcaaaact
    35581 ttagaaatta gtttttcttc agaaggaaaa cgttcctcta gtaaaggacg tacttgttcc
    35641 acaaacattg gtttcaactc attaggcgga ccgggaagaa ggatatacgc atgtgtatct
    35701 gtttgataaa aagtccctaa agcaagccct gtacgatttg gtagaggaac cccgccttcg
    35761 ataatctggg actgttgcag attgtttttg gtcatttttc gatgggttgt ttcaaaatat
    35821 gccaataatt ttttgtatcc ttccgtattt tgtatgagag acttaccgag gtgagccgca
    35881 gttgcttctt ttgtcaggtc atcttctgtc ggtcctaatc cgccacataa gagtatgagt
    35941 tcacttcttg tttcagctag agcaatcaac tcttctaaac gctgctgatt atctcctaca
    36001 actgtttgat aataaacttc gatacctaga tcagctagct gttctgacaa gaaagtggca
    36061 ttcgtattca cgacttgtcc tagtaaaagt tctgttccga cagctattat ttctgctttc
    36121 attacttccc gctcccttat atagtatacg ctgtattttt atattttttt atttaaaaag
    36181 agggcaagga gctgtgacaa aaatcttgtc acagctcctt gttccaaata aacagtgttc
    36241 tcctgccaaa acctcggtca taagtcacaa tatacataaa acatgaaaag ctgttttttg
    36301 aaattatccc ttgtgactca gagttagacg gcaggctcac aacctctcca ctaaacgata
    36361 cttacattga tcctttgaac acaccgctgt ttttgatgaa gtaatccaca cctgaataga
    36421 tagtaaagat cagacaagca taaagcatga tttgatctac tggaataccg atcaggctga
    36481 atgggatatt attgataaac agcaagataa tagcaaccat ttgtgtagcc gtcttgactt
    36541 ttcccggcca agctgcagcc atgacttctc catgttcgac aagtagtaaa cgcaagcctg
    36601 tgacggctaa ttcacgacag acaatgatcg ctactaccca accaggggcc tttccttggt
    36661 caaccaacat gataaaagca gtcataacta gcattttatc tgccaacggg tccgcaaact
    36721 tgccaaaatt agtgacaagg cctcgcgcac gggcaatttt tccatctaac cagtctgtga
    36781 tactagctaa agcaaaaata actgcaccta ctagctgtgt gacagccagt gtggtgtctc
    36841 ctacgttaaa ttgtccccaa tctaaaggta aacctacaac caaaatgaaa atcgggatca
    36901 tacaaattct aagtacagtt aatttatttg gtaaattcac actatcgact cctttcttcc
    36961 ctattctacg tgactatcag cgaagtttcc accgatatta tttacttttc caaatgaatt
    37021 tattcaaagg agtattttta ttgaaattga tctttttgaa ttaacgaaga aggaccgcga
    37081 cacagatttg tcacgatcct tcttttttat acctacaagt aaacagatgc gtaaactaat
    37141 ttatacgatc attctgcgta ttgaagagta aggttcacat tttttggact ctgattgttt
    37201 tcccctggat taactggtac ggattctcca ttcgctttga tcgttgcatt gcttgctgca
    37261 ccaatcgtga tggtcgcatt tgttgctgtt tccggtaaca cggtactctg tgtttcattt
    37321 gctgccagtg tcccttgata aacataggca ttgtctacta gcacacctaa ccatacacga
    37381 ttgacagcag taaattcaag ggttacaggc ttggtagcgt ctgtgatatt tactgcgatg
    37441 gcactgccag aatcttctcc tgtagctatt gccattttct tttcttcact tgattttgtt
    37501 gttgattcta ctgtagaaga ctctttggtc tgcgtgcttt gctctgagga agaggactcc
    37561 tcgactgatc cttctacttt aagcgaagaa gctgtgtctc cgattattgg atcagcttga
    37621 cgatcttgcc aagtcatata gcccacgaca acgacaatcg ccaaagcaac caatcctaat
    37681 aaaatgacag gaagacttgt ccaaaagcga cttggatgcg attcttcctg atgcattgct
    37741 tttctagaag tacttactgt ttcaggctgc ggacgttcag ggaccgatcc tgtcaatttg
    37801 cttttcccat caaaaacatc gactaaatgg tctccatctt ctcctacagc ttcagcgtat
    37861 tgccgaataa atgcccgcac gtaaaattta ccaggcaatt ggtcaaaatc atcattgtcg
    37921 attgcttcca aataacgttt ttgaattttt gtaatctgct gtaattcatc taaagatatt
    37981 tttttattca ggcgtgcttg ccgtaatttt tctccgatgg tttcaaaagc cactcgttca
    38041 tcttctccag tctgtttgat tctccgaagc atctgctgtt gcaacgaaag acgttatgtt
    38101 tgggtgctgt gacaaaagtc atgtcacagc tccttttttc cgaataaaca ttgttctcct
    38161 gccaactcct cggtcacaag tcacaatatc caataaatat aagaagctgt tttttgaact
    38221 tgttccttgt gactcggagt taaacggcag gctcacgacc tctttcttaa acggtgttca
    38281 atcagctgtt cctcggtatt agctcaaaaa ccggcaaaat ataaggagat atttttccgt
    38341 atgtttctct aatactcagg tcaaaacgct gatttcacaa cctcttcctt aaacggtgtt
    38401 caaaagcttg catctgcggt aataagtcca accaagttaa aaacattaag aagctgtttt
    38461 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct tggtacacgg
    38521 tgttccagaa gtagcttctt cggcaataag ccgaaatatc acaaaaattt gagaagcaat
    38581 tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tctttgatta
    38641 cttcgtttga ttttatttta atataagttg attaaaagag agcatgattt gttttatttt
    38701 ctcactaatt tttccaacac aactaagaat accataaaag ctgattttgg aggaggttcc
    38761 tactacaagt ctaaaacgaa atttaagact tttcctcata aaatactggt ttattttggt
    38821 ctcatgtaaa actcgctgaa ggcctcttca cttataaatg cttccccagc tgccagtacg
    38881 tctttcattt ggatcgaatc gatgatttca ggaagatcaa ataaagttcg atcgccaaat
    38941 aaactttgcg taaattgatt ggcaatatat tcgatcgaat ttaatgattg gaaatattgt
    39001 ccaagcattt tcttcttcag caaatcgaga tttgtttctg acacttccgg atcatcagcg
    39061 aatccaagaa tgatttcttt gactttttct gctgctttct ctggttcgtc cgtatctccg
    39121 ctgaagtccg caaagtgaaa ttcacgatcc aagctgaatt caaaaccaaa gctgtcatcg
    39181 ataattcctt gattgtacat cgctagatag tttcgagaag tattgcctaa aatcatttgg
    39241 aacaacagat tgattgctgt tttatagcgt aacagttctg ttccttcttg cggcaatgtg
    39301 tctaatcctt taataccaag tacaaattta tctcgtgtaa tggctgcttc caatgcactt
    39361 tgtttgatga tttcttttgt gttttcaggg aaataacgta caatttcttg ctttggaggg
    39421 aaattttttg cttcttggtt ttcccgaata agtttcatca atttctcagg ctccatctta
    39481 ccaactacaa ataaaaccat attgcttggc tgataaaacg tccgatagca cgtatacaag
    39541 tcttgagctg tgattttgtc gatactttca accgtgcccg caatatcgat atgcaatggg
    39601 tgtgtaggat acaagttatt tagtatgcca aaaaacatcc gccagttagg gtcgtcttca
    39661 tacatttgga tttcctgacc gatgattcct ttttccttgt ttacagtttc ttctgtgaag
    39721 taaggtgctt gtacaaaatc gatcagagtc gtcagattct tttctacctg atccgtcgta
    39781 gaaaacagat agctggtttt cgtaaaactt gtgaaagcat tggcggaagc tccttgtttc
    39841 ccaaataatt gaaagacatc accatcttct ttttcaaaca acttatgttc taaaaaatgg
    39901 gcaattccat ctggaacttt caccttttct ttttcaccat agggaataaa ttcgttatcg
    39961 atcgaaccat aattcgtact gaacaaacca tatgtcttgt gatagtcatt tttaggcaac
    40021 aaatacacgg tcaaaccgtt tggcagtact tcatgataaa gtgtttcatt gatttgctca
    40081 tattctgttt tattcatttg catttcctcc tgctaaaaag aagattgcct gcaattcgat
    40141 ttgctcagct acttgttgta tctctttgat cgttacgctc tccatacgtt tcaaccattc
    40201 ttcatcagat aattttgttt ccggcagcca gctgtccaaa aaagaagctt cgattgccgc
    40261 ttgcggagag tctagagaaa gcagatattg gtttctcagc attgcttttg tctgagacag
    40321 ttccaactct gttatttcac catttcgcaa actttctaat tgctcgtgaa tcagatgaag
    40381 tacttgattt cggttctttt catcgatacc ggtttggaca ctcatgaatc ctcggaacgt
    40441 atccacgcta ctagaggcat aataagctag actttctttc tctctgacat tcatgaaaag
    40501 tttagagtga ggaaaaccgc caaataaacc gttaaagacc attagcgcaa aacgttctgg
    40561 ttcatcgtaa tacacatttg tctgataagc taggttcagc tttgcttgaa cgattggctc
    40621 acgtacttgt cgttccttca cgatattcac ttgaggttgg gtataaaaca gatctggata
    40681 aactgtttcc cgcgactcaa atggtaattg cccaactgct tcagctgctt tgtccggatc
    40741 aacatcgccg acaacaaaaa tgtcgatttg atcatttgcc atcatttctt gatagtagga
    40801 cgctagcgaa ctagcagtga tcttgtccaa atcttcgact acgcccaacc ctgggatctt
    40861 ttgttccggg ctgttttcaa aataaagttc ttgcaaggca taggaagcaa aagtttgttt
    40921 atcttccttc aaactttcca aatatagacg cagattgttc ttttccaagt caaacgtcaa
    40981 ctgatcaaac tgatgatttt taatatttgg ataaaataaa acctctttta aaaaatctac
    41041 cgcttgagaa aaaaggtttg gatcattgac atattcacca ttcacaaaac tgatccctgc
    41101 gtttagccaa tgtaaatttc ctttttttct aactgataat ccaaagcttg cgccataaag
    41161 ctctgcaagt tgcgcactta actttgtctg atccgaataa ttcaaactat ttgtttccaa
    41221 catgctgctg agaagtgaac gtttcgtgat gacttcttgt tgcagacgcg cagtaaaacg
    41281 gataaaaatc cggatcgttt tgtattgttt tgttggtaag acatgaagat tcacgccttc
    41341 tgccaattga tacaccatat ttatatgctc cttttcacag ttgattgccc gcttactctt
    41401 ttaatgggtc aacctgcctt ttttacttat tcctctgctt gaaatttata agttcaggca
    41461 attcgccgtt ttttctaaat gatgttacac tttatagtat agcatagatg ttttttatca
    41521 aaagaaacag acggctaaaa gcgtatattg tttttttatt ttttattttt cgatatacta
    41581 aagacaaata aaagaaggga gggtcattca tgatcaaaga attcaaagag ttcatcatgc
    41641 gtggagatgt cttggatctt gctgtcggcg ttgttattgg tagtgcgttt acaagcatcg
    41701 taaaccaagt ggtgcaaggc ttgatcacac cactaatagg ttggtttgtc gcaatgatca
    41761 caggtacttc tgatttagaa ggagcacttt caattcttga ctggtcgccg acaaaaggcg
    41821 tcacatttgc gtttggtaac gttattagtg ctatcattac atttctgatc actggttttg
    41881 tcttattctt agttgtcaaa gcggcaaacc gtgcgaagaa tttacgctcc aaagaagagc
    41941 aagtggaagt ggaagaaact cctactgcag aagattatct tagtgacata cgcgatcttc
    42001 ttgccaaaca agtggcagaa gacaatacac atccagtaga gcaaacaaat ctttccgata
    42061 cttctaaata ataaagaatc taaaaaaaaa gaaagccgat tttcagtgtc ggctttcttt
    42121 ttttagattc ttaacttgct acttgttcct ttttcatgtc gatatgtgga atatcatctt
    42181 ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct tgtaggtatt
    42241 cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg ttaatcgttt
    42301 cttgaacaat ctttcttcct aaaccatctt tacggttgtc ctgatgaaca aggacacgtc
    42361 caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc ttcccatcag
    42421 tatcttgaaa aaacgtatgc caagcttctc catcaatgtc atcaacttct tgataaggac
    42481 actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa aactctgctg
    42541 ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc cctgcttttt
    42601 tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa tctcaggctc
    42661 gtttgctttt taaactgctt cagttaaaaa ttcgatctca actgtccgag taagtacatc
    42721 tgaatagctg taagatactc gttcaaaaga attttcttca ggatccaaat caacaacgaa
    42781 tactgaagga taggtttctg ttaaaacgcc gcggcgttct gtttgacgct ttcttcctgt
    42841 ctgagcaacc aacatgattg ggctgccgat acgaccttct aaatctttct tgattgaagc
    42901 taaagttgtt ggcatacagt tcacctctac tatcaactat accacatatt tttaaaattt
    42961 tcaagtttac cataaaaaaa aaagaaatgc aataaaaata aataagaagc tcattaagat
    43021 ttttctaaat tattcctcat tataaaattt caagtgtttt tttattttga gttttactct
    43081 cccgtaagcg ctcattactt gcttgtgatc cattgctaaa cattcagcca tgtctacaaa
    43141 atcaacgcct tgcatataat aatgaaaaat tttagcttct atttttgaaa actggatttc
    43201 gcaattttcg attgcttctt ttaataaaag atgctgaatt gctaaaggtt cttgcaatgt
    43261 gatctctgct aaaaagtctg gtccttctgt ctctattttt tggtcgattg agacttcatc
    43321 tttttgatca cgacgcttca gcgcgttctg cttcctcaac agagaataga tttgattgct
    43381 tataacgctt ttataataac ttccaaagct aacacctttg cttttttcgt aagttctcaa
    43441 tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc tcagaaacat aatttcttaa
    43501 ataataggtg tttttcaaac gtagtaaaag tggaaaatat ttttgataca gggcagcaaa
    43561 tgcttcttcg tcaccattac gtgcagcgcg caataactcc tcagacataa aataattcct
    43621 ccataaattc gtttatggag gaatcttatc atcaatgatt catcgaactg attagaacga
    43681 tatgttattt attttttctc attaaatcgt ccaatttttg ggacaatttt tttaactgct
    43741 cttcattcca tggagaatta cgtcggaaat caataaaatg catatcttta ttttcttggg
    43801 ctattgtttt ttccgttttt tttatcgtct tatataattc ataagcagaa gttcgtaatg
    43861 ctccttggga aaagatcatc cactgttctg ctaaatcgct tgtcgccact gtcacttgtg
    43921 tcaaacggtc attcagctga ccagcgattc tctcgatata actgtcagcc gtttcgtctt
    43981 ctttcgtaaa aacaacatcc aactggtatt tcttataatt ttgttggatg cccggaacta
    44041 gttgggcatc gaagacaacg atgacttcca ttccttcata tttcgcataa tttgataaac
    44101 gatgtaaaag agcttctctt gcatccgcca ttttgtcctg ctttttaagc tggacaagtt
    44161 ccggccacga accaatcatg ttgtagccgt ctacaatcaa cagctgtttt ttcatggtct
    44221 actccccctg acgttgggaa aaagcctgat acataagcaa accagcagca acactggcat
    44281 ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa ttcatccatt tcttttttca
    44341 atcctggact cattcctttg ccttcattac caatgactaa agcaattgat cctttggcat
    44401 tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa tacccaaaat cctgtttctt
    44461 tgagctgttg cacagcttgt accaaattcg tcacgcgtgc aacagggaca tgttcgactg
    44521 ccccagtgga agtctttgtt acgataggtg tgattccaac tgctcggtgt tttggtatga
    44581 tcacaccatc gacgccactt gcgtctgctg tccgtaaaat cgacccaaaa ttgtgtggat
    44641 cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt tttctctttt gttcgttcaa
    44701 tcagttggtc aagggataaa tattcataag gtgtaatcgc aagaacgact ccttgatgca
    44761 cgccgtgatc gctcaaagtt tctaattttt gtttaggaac ccatttgact ggcacagctt
    44821 gttcacgtgc gatttctttc agttcttcta ctttttctcc tcgtgaatct tcttgaagaa
    44881 agagtttatt ccctcgccct tgttgtagcg cttctactgc ggcatggtgc ccaaagacga
    44941 aattatcttc taatgcttct tcattttgat cagaaactga ctgatttcgt ccagtttgct
    45001 ttttatttga atatctattt tgttttttgt cttcaaatcc acgtttcgat tgatgtttgt
    45061 ttgttctttc tttacccatt tttttcacca actttctgaa tacaccagcg gatcaattct
    45121 tccatccgct cggtttgttt tgtcaaatgc agatagccca tgagcgcttc gaaccctgtt
    45181 gcgacccgat acgtcgtaac atcagcattt tttgcgctcg tatggctttt agcatttctt
    45241 cctctgcgat aataaagttc ttcttcttct gtcaaaagat gttcttccag catttgctga
    45301 atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg ccgcacgatg cagcttattg
    45361 ggttttgttt ctccttgatc aatcaggtgg tcacgaacat agatttcgta aatcgcatct
    45421 ccaacataag ccaaggctaa accgttcaat tgtgtgtaat ctctcatgct tctcttctcc
    45481 atcgggttcc ttgcggtgta tcttctaata agatcccttt ttctttcaac aaatcacgaa
    45541 tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg atttcgttct tcgatcaatt
    45601 gttcaatctc ttcatctaat aactcttctg ctgtaaataa aataccgaag atcgaaagcc
    45661 attgcgagaa taaagcttct ccttgtgcca agacagcggc agaaactttc ggctggtcta
    45721 gataagtgtt cagccactta gcaagctcat agaccactgt gatcccattt gctgcattaa
    45781 aatcatcgtc catttcttcg atgaatcgct gttctaaatt ttctaacaaa gcgattttat
    45841 ccgcatcatc tgccaaattc tctgaggcag tttcttggcg gaatttcaaa ttgtcaaacg
    45901 tattcttcag tcgttgtaaa ttggtggctg cttctttcat cgtcgtttca ctgtagcgga
    45961 tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa gacttgtgga tcaacgtttt
    46021 tgatcatttc atgtacagtg atgaaattcc ctagagattt actcattttt tcatcgtctt
    46081 cgccaatcgt tacatagcca ttgtgcatcc agtaattagc gaattttttc cctgttttcg
    46141 cttcactttg tgcgatttca ttttcatgat gaggaaattc taaatcttgc ccgccaccat
    46201 ggatatcgat cgtttctcct aaatgttttg ttgccataac ggaacattca atatgccaac
    46261 cgggacgtcc tttgccccat ggtgaatccc aagaaatctc gccaggtttt gcttgtttcc
    46321 ataaagcgaa atctaatggg tcttcttttt gctgctgttc ttcccctgta cgctgacttg
    46381 ctccgacttc cagttcatcg atggattggt cacttaattt cccataattt tcaaatttac
    46441 gggtacggta atacacatca cctgaagatt cataagcata gcctttttca accaacactt
    46501 gaataaatgc taaaatatct ggcatatgat ccatgactct tggatgcagt gtagcaggtt
    46561 cgacattcaa cgctttcgta tcttcttcaa atgctcggat gaaacgatca gcaacttctg
    46621 gtgctgtaat acctaattcg tttgctgtac ggatgatttt atcgtctaca tccgtgaaat
    46681 tcgatacgaa atcgacttta tagcctcgat attcaaaata gcgtcgaatt gtatcgaaag
    46741 caatggtgct acgagcattt ccgatatgga tataattata cacagtcggt ccgcagacat
    46801 acataccgac atgtcccttt tgtataggga tgaattcttc tttttttctg gtcaatgtat
    46861 tgtagatttt tatcattctg atctcctcac tttttctcct ttgattcgaa cgacttttgc
    46921 tggtactcct accgctgtag cgtcgtctgg aacatctttc aacacaacgg cacctgcacc
    46981 gattttggcg ttttttccga tcgttaccgg tcctaatatc tgcgcattcg cagagagcat
    47041 ggctccttgt ttaacagttg ggtgtctttt acccgtttct tttcctgtgc cgcctaatgt
    47101 tacgccatgg aataaaatca catcgtcctc gatttctgcg gtttcgccaa tgacgattcc
    47161 cattccatgg tcgatgaaca cgccagttcc aatcgttgct cctggatgga tctcaacacc
    47221 agttaggaaa cgccagaatt gagcattgat cttcgccaat aaaaagagcc ggtgctgata
    47281 taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa ccagggtatg tcaaaacgat
    47341 ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct gcatcaatcg ctcgggtaaa
    47401 ccaactcatg actttccact cctttcgtaa gaattttatg taggaaatca tcttcttata
    47461 aagaaaagaa gcgcctttta aataagtttc cttatcaaaa gacgctttcc gcgtggttcc
    47521 acttttcttc ataatcaatg attatctctg acagctaacg cctgttatac gttcttggct
    47581 actttgtttc ctataaaaca gtttttcctg ttttctgtgt ttcgccaaga ccactcccag
    47641 aggcatttcg gcaaccattc attgatacct tgcaccaccc ggtatctctc ttgtaatgat
    47701 cagctgctta cttttttctg ttcatcgtgt ttagttcagt acttgattta aatgtgctaa
    47761 tgatttttca cgaccaagca attcgatcgt ttcaggcaat tctggaccat gcatttgtcc
    47821 tgatacagct acacggattg gcatgaaaag attttttcct tttacgccag tttctttttg
    47881 aacagctttg atggcagctt tgacagttgg agcatctact acttccattt gttctagctg
    47941 tgctttgaat gcgtggagaa cagtaggaac tgtttcacca gcaagtactt cttttgctgc
    48001 ttcattcaat acaggatgtt cattgaaaaa tagcgtagac agatcaacga tttctgcagc
    48061 atagctcatt tgaggttgat acaggctaac gatcttcttc acccattcga ttgtcttttc
    48121 atctgggttt tctgctacac gtccatcagc gattagataa ggcagacaca tgtcagtaaa
    48181 tgcatctaga tccattgctt tgatgtaatg gttgtttacc cattgcagtt ttttcgcatc
    48241 gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac attttgatca attcttggtg
    48301 ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat aatgcaataa agttgaacat
    48361 tgcatctggt agatagccta attcgcgata ttgttcgata aattgcaaga ttgattcgtc
    48421 acgtttgctt agttttttac ctgtttcact gttgatgatc aatgtcatat gtccgaaggt
    48481 tggtggtgtc cagccaaatg cttcatagat catcagttgt tttggtgtat tggcaatatg
    48541 gtcatctcca cgtaaaacgt gagtgatttt catcaaatga tcatcgaccg caacagcaaa
    48601 attataagtt ggcataccat cgcgtttttg gatgacaaag tcgccaccga tattatcgga
    48661 ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg tattctgtat tgcgaggcac
    48721 acggaaacgt acaacagatt ctagaccttg tgcttctttt tctgcttgtt cttcaggtgt
    48781 caagttcgca catgttcctg cgtaatgagg catttctcct cttgcgcgtt gtgcttcccg
    48841 ttctgcttct aattcttctt ctgtgcaata gcatttgtat gctcgattgc tagcaagcag
    48901 ctgatcaatc agcggttgat agatttcttt acgttcagat tgacggtaag ggccgtattc
    48961 tccaggattt tcaggagatt catcccaatt cattcccaac caagcaaggt tttccaactg
    49021 gcttttttcg ccatcttcga tattacgttt ttggtcagta tcctcaatac gaataataaa
    49081 gtctccatca ttgtggcggg caaacaaata attgaataat gcagttcttg catttccaat
    49141 atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt actttcgtca ttttcttcct
    49201 cttctttcaa actagacttt gactaacagc aaaaattccg tcagttacgg cttctcaaat
    49261 atcatttatt taaagccact cagcacaaat tttacctttt ctaaggtaaa tagtaaagcc
    49321 aactggttga atttccttac cagttggctg aatttctttt ttattttgat tgtttagtct
    49381 ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc atgtgcaggt ttagcaaaga
    49441 tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac gacttcgata cgttcattca
    49501 tgtaatgctg tccatcttct acgacaacca tcgtgccgtc atcaaggtag gctactcctt
    49561 gttggcgttc tgtcccggct tttactacta gaacattcat cgtttcacca gggatgacga
    49621 ctggttttac ggcatttgcc aacgcattga tattaagaac aggtacattt tggaattcag
    49681 agactttatt caagttatag tcattggtta caatgacacc atctaaaagc ttagccaatt
    49741 tgatcaattt gctgtctact tcactgatat cttcaaagtc tccatcgtac atttcgacag
    49801 atataccctc ttctttttgc aaggcattta ggatatctaa tccgcgacga ccacgcacac
    49861 gtttcaaact atcccctgag tccgcgatgt attgtagttc gtaaagaaca aaattaggga
    49921 tcatcaaggt tccttctaag aatccagtct ttgtaatatc ataaatccga ccgtcaatga
    49981 tcacgcttgt atccaagatt ttatatttat ggaaattttc tcctgatttt cgttctaaca
    50041 gttgactctc aacttgacta gctgtttctt gctcgatttt tttgcttcgt gaaccaaaaa
    50101 ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa tcccagatag cctaagatga
    50161 tcatcagtaa aatcggcaat acactattga cgaatggaat ttccatattg taaagagggg
    50221 tcgagatcac aacacctaaa gtcaatccga tgatcgcacc gatcgcaccg aagagaagat
    50281 aagtcaagct gacctcactt agtttttgtt ctacttgttt gataatagct gagatatgtt
    50341 tggccaacag caaagataat aaaaagaaaa taagtgcgcc taacaggctg ttggtaaatg
    50401 tattattcag ccatccgttt gactgttgac cgattgcctg ccaagctgag ggtaaaaatg
    50461 aaatacccaa gcttgcgccg acaatgatca tcagcaaagt aatgacacgt ttttgcatcg
    50521 tttttcctcc gtttcgacaa tcatttatta gttagcggaa cacacgcttc aacgtttcag
    50581 ccaaagttgc tactccaact atctcgattc cttttggcgc ttcccagtta cctaagttgt
    50641 ttttaggtag gtatactttt gtgaagccga gtttttggac ttctttgact cgttgttcaa
    50701 tatgactgac acgtctgatt tctccagtca gtccaatttc accgatgaaa cattccgttg
    50761 gctgtgttcc tttttcttta tagcttgaag caatactgac agcgatagct aaatcaatcg
    50821 ctggttcatt taattttacc ccacctgcag cttttaagta agcatcttga ttctgaagaa
    50881 gcaacccagc tcttttttcc agcactgcca tgatcagaga tacccgattg aaatcaagac
    50941 ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt gactaatgct tggatctcta
    51001 ccaaaattgg acgtgtccct tccattgcca caacgatagc agagccagtt gcaccgtcta
    51061 ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc ttccaatcca tgctcatgca
    51121 tttcaaaaat cccaatctca ttcgttgagc caaatcggtt ttttactgct cgcaaaatcc
    51181 gaaaactatg atgttgttca ccttcaaaat ataacaccgt atccaccata tgttctaaca
    51241 ttcttgggcc ggcgatcgag ccttctttgg taacatgacc gacgataaaa atggctatcc
    51301 catttgtttt agcggttttg agtagttctg cagtggtttc tcttacttgg ctgacacttc
    51361 ctgccacact tgtgatgtct ggctgcgtca tcgtttggat ggaatcaatg ataacataat
    51421 caggggagat atgttcgatg gcacgactga tttcgttcat atctgtttca gcgtatagat
    51481 aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa ttttatctgc tctgcgcttt
    51541 cttcgccgga tacatataag acttttccgc ctatggctgc taattgctga gaaacttgta
    51601 acagcaaagt ggatttcccg atacctggat cgccaccaat caagaccata gaccctggca
    51661 ccacacctcc gcctaacaca cggttcagtt cttctaattt tgttttgatc cgaggttctt
    51721 ttttagggac gacgtctgcg atttttgttg gttttgcttt ttctcctgtc aaacttgtcc
    51781 gagttctgcg gtccgttgta tcttgttcga tttcttcgac catcgtattc cattttccgc
    51841 aatttgggca gcgtcccaag aacttaggcg acacataccc gcaattttgg caaacaaatt
    51901 gtacttttga tttttttgcc attgtaccgc ctccttttat cctaatcatg agacattaaa
    51961 tcaagcttaa gtatctttaa cgttttattt actggaagaa ccaaatccgc cttgacgttg
    52021 agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa aagatccctt ggccgatacg
    52081 ctcgcctttt ttgatcacta catccttgaa accaaagttg gtgaattgga acatgatatg
    52141 gccttcattt gcctcattat tgtaataatc actgtcaatc acacctactc cgtttgtcaa
    52201 cactaaaaac cgcttcaacg gattgcttga acggttagca agctgcaaaa attcatcttc
    52261 tcccatgtaa gctttgattc cagtaggcac gagggttggt ttcaattgtt tttcattttc
    52321 tgatacatct tttttcaatc catctgcgac tgtttccaac ccctttttcc agcctgcttt
    52381 ccagatactt ggaatcacga cctcttctgc tgcttcaaaa tcatagccag cagcatgatg
    52441 tgtagcgcgc tgcggtagat tgatattttg atctttatat ttagaaataa cttcaaatcc
    52501 tctttttctc accggttttc ctccctaaat aaactactcc tattctaaca tatccgaggc
    52561 tgtaaaagac tttctttaca aattcttcaa gaaattctgc tatcttaaag taaaccatta
    52621 attcgttctt tttccaaacg actgcaagtt gtacaaaaaa actatttttt acatgacttt
    52681 actcgtcaaa acaagtagaa tagaacgaaa ttaatgagtg gatatttgat tgaggtgtca
    52741 catgcgtaaa gaaattaaaa atgcaaaacg aattattgtc aaagtaggaa caagtacatt
    52801 gatttatccc aatggaaata tcaatcttgc ggcaatcgat cagttagctt ttgtcctttc
    52861 tgatcttaga aatcggggaa aggaagtgat cttagtctca tcaggagctg taggcgttgg
    52921 tatgcataaa ttacatttgt cagaacgtcc gactttgatt cctgaacagc aagctgcggc
    52981 ggctgttggt caagccgaac tgatgaatat ctataatcaa agattcagtg tatataatca
    53041 acagacggca cagatcctcg tgacaagaga cgtggtagat tatcctgaaa gtcgtaaaaa
    53101 tgtgaggaat acattggaac agcttttgat tatggacacg atccctatcg tgaacgaaaa
    53161 cgatactgta gcagtggatg aattagatca tctgacaaaa tttggcgata atgatcagct
    53221 gtcagccatc atggcaaaac ttgtcgcggc agaccttttg atcgtccttt ctgatatcga
    53281 cgggttttat tcagataatc cgactacaaa tcccgaggct cagatgtatc gctatatcga
    53341 taaaatcgat gaaaagattc tggcacaagc aggtggtgct ggtagcacat ttggaacagg
    53401 cggtatgcag agtaaattaa aagccgcttc taggatattt gaagcaaaca gcgcaatgat
    53461 tttagcaaat ggaaaaaatc cggcaattat ttttgatatt cttgcaggaa aagaagtagg
    53521 tactttattt aaggaggact tgaaatgact gacttgatgc aattaggaca acaggcaaaa
    53581 gatagcgcgc aacaactagc acttatgcct acaaaccgca aaaatgagct acttgttcaa
    53641 atggcacaaa ccatcaaaaa gaatcaacaa gcgatcattg aagcaaatca taaagactta
    53701 gaaaaagcag tcgaaaacaa tatcagtgaa acgatgcaag accgcttgca gctgacacct
    53761 gagcgaatca atgccatggc tacagagatc gaaaaaattg cttcgttaga cgatcctatt
    53821 ggaaaagtag atgagatgtg gacgaacacc gacggattga gaatcggcaa aaagcgcgtg
    53881 ccactaggcg tgatcggcat catttacgaa tctcgtccaa atgtcacgac agatgcagct
    53941 agtctagcat tcaaatcagg gaatgccgtt atccttcgcg gtggcaaaga agcattcttc
    54001 tcgaatcaat tattagtcca attattgcag caagtactgg tcagtgctgg cgaatctcct
    54061 tatgccatcc agtttgtcga tgatacttct catgaaactg caggacagtt gatgcgattg
    54121 acagaatact tagatgtgct gattcctcga ggcggagcaa aattaatcca acgagtcaaa
    54181 gaacaggcaa cggtacctat cattgaaaca ggtacaggaa ataatcacgt atatatcgat
    54241 aaagaagcac agttatctat ggcagtgaac atcatcgtca atgcaaaagc ttcccgtcca
    54301 tctgtctgta acgcggcaga aacattgctg atccattctg agatcgcacc ttttttcttg
    54361 ccagctattg aaaaagaact ggtagaacac ggggtctctt tacgtgcgga tgctcgtgcg
    54421 ttagaatatc tagagacggc tgctttagca gaagacacag attgggatac tgaatatcta
    54481 gattatatcc ttgctgtcaa agtggtcgat tctttggatg aagcaattgc acatatcaat
    54541 cggcataata ctaaacattc agaaacgatc gtgacagata gttatgcggc tagtcaacgt
    54601 ttcttaaatg aagtggacgc ggcggctgtc tatgtcaatg cctccactcg ttttacagac
    54661 ggatcggtat ttggttttgg tgcagagatt ggtatctcta ctcaaaagtt gcatgcccgt
    54721 ggtccaatgg ggttaaatga attaacttcc acaaaatata tcatttatgg cgatggacaa
    54781 atccgcgaat aatgtttcgt gtgaaacatt attcccgcct tttgcctttt ttctgtaaag
    54841 gttctatcca actagaaagg tgtcaatatg tttgaaattt taatgatttt ttttatttac
    54901 cttatttctc tgaatatcgc tgcttttctg ggagtcggta ttttatccct tttctttcaa
    54961 ttcaaaaaac gaagcatagg gagtcaacgg gaaaaatggt cgcagtattt tgacaaaatc
    55021 gggcaaaaag ggctggttac acgcttgcac attagttaca tggttgcatt atgcttatta
    55081 gcgacaatca attactattc ttttttcgat cattcaatcg cttatacgat tacgctactg
    55141 attgctggga tttttcactt atcttataaa tatcagctga ataaaaacca cttgaatcgg
    55201 actttccgct aaaaaatatc cgtacgataa gtagagtgcc ttgaagatcg gcaaaaaact
    55261 gccggacttt ttagaggctg tgacaaagat tttgtcacag cctctttttc catataaacg
    55321 gcgttcgcct accagctgcc tggtaaatct gtttcctctt cgttaagaag cgaaaatccg
    55381 tcaaatgttt cgccatcata ataacgaagc agctcatcca tggacaagtt catcgttttt
    55441 tctttttctt cttccgctga ttcagccagt agctgttccc gatacacctg atacatacca
    55501 gctatttttt gctgcatcgc ttttctagtt tcaccttccg cattgattat tggcaattct
    55561 ttgatatagc cacgataaat caaccgatca tctgaaaatt caatgggtaa ttcaacgagt
    55621 gtcaacccga cttcctgaaa atattcttcc agcatctatt atccccccat tctttttctt
    55681 aaatgaattt tagcttacct actgaaaatt ttccagtttc attattttga gaaaaaaagg
    55741 gaatgtgaca aaaatcgtgt cacatcccct ttttccgaat aaatggtgtt cttctgccaa
    55801 ctcctcaatc ataagtcaca atattcaaca aacatgagaa actgtttttt gaaattgttc
    55861 cttgtgattc agagttaaac gacagtctca caacctcttg gtaaacggcg ttcaatcagc
    55921 tgaccctcgg tattagctca aaaaacggta aaatatgaga agctattttt ccgttttgtt
    55981 tctctaatac ttaggataaa acgctgattt tacaacctct gttaaacggt gttcaatcag
    56041 ctgtccttcg gtattagctc aaaaaacggc aaaatatgag gaactatttt tccgtttgtt
    56101 tctctaatac tcaggacaaa acgctgattt tacaacctcc tgataaacgg tgttcaaaag
    56161 cttgcatctg cggtaataag cccaaccaaa cgaaaaatat gagaagctat ttttgtttgg
    56221 ctgttcttat gacttgatgc aggacagctt tttcacaacc tctgatttct tattcttctt
    56281 catttgtagc aaaataatta cggtgataaa tgatacaaca tacactgata agaacaatgg
    56341 cacctgcaaa ataaaacgca tagctttcac caatatgttc accaatcaca cctgcaataa
    56401 atggtgctaa agctccaccg aaaaagcgga caaaactata gctggacgaa gcaacgtttc
    56461 gttgcagacc tggtatttcc atagcaatcg tcgtcaacag tgtattgata attccttgga
    56521 aaaatcctgc taacacaatg ctgatcggta caaggactgg catagaagtt ccagctccta
    56581 ataacagcaa acaaataatg aataagacaa atgctgctaa aattgtccga taagtcgtaa
    56641 acttccgctc aagagccggt gcaacgaaaa tcgaagcaat agccagaagc acaccccagc
    56701 caaaaaatac aaaaccgact tctaattcag aatagccaat caataaaaat ggcgcatacg
    56761 caagtaaagt aaagaatccg aaattatata acaatgcgat tagtccaact gaacgaagtt
    56821 tatgattgct taacgctttg acaccttccc aaaggcctac ttttctcttt ggtttctcag
    56881 tttcaggtaa aagtaccgct agggctataa aaccgcaaaa catcaaaaat gctaccccaa
    56941 aaaatggata acgccatgaa ccactgccca agaccccacc gactagaggg ccaacagcca
    57001 ttccgcagcc aagtgctgct tcatacatca tgattgcttt ttctgtgtta ccaactaaaa
    57061 tactgacgat cgttgctaaa gcagtagaga caaataaagc attcccgaca ccccagccgg
    57121 cacgtaaccc aatcagcatt ccgatattcc cagataatcc gccaagacca gcgaatacaa
    57181 cgatgataaa caatcctgtc atcaatgttt ttttcaaacc gatccgacta gataagaaac
    57241 ctgtaaatag catcaccaca ccagtgacta acatgtaact agtaaataac agggtcgttt
    57301 cagctggcgt agcgtttaac tgtgcagcaa tcgtttttaa gattgggtct actagaccca
    57361 ctcccataaa agcgatcata caggtagctg ctacaactag cgctgaactt ttcccctttg
    57421 tttcttcatt tgtttgttct tgtatatcta tttgattctc caatatttcc atccctttct
    57481 ctctattagc atcatacatg atttatttta catgtcaata tgcacatgta aaacgtggaa
    57541 actttactgt gatataataa agaaaataag acgagaggta ggatttttat gaatacttta
    57601 gcctatatat tattatgcgt gcttttatcc aaaccacgtt ctgggtatga attgaagcag
    57661 ctgatcacaa ttttttggga agcccatcac agtcagatct atacaacgct tgccaagtta
    57721 gaccagcagg gttatgtgac cgttttggac aaagacgagc actctcaaaa aaagatctac
    57781 cacttgactg atgaaggacg tgctcttgta gaggaatgga tcaaagagga aactcccgct
    57841 cctacgcaaa aagatgaatt tctagcaaaa atctatgctt tttcgacgct cgataaaaca
    57901 acagctactg gactgctatt cactagagaa caacagctga acaaaatcat gcaaaaaaat
    57961 caaaaaaaat tgaatgacct ctcgctagac aagaaacaag aatttggacg ttatgttgtg
    58021 atccagcgca gaattatgct ttgtcagcag gaactccaat ggtgtcagca agtaagagaa
    58081 caaatgcagc agttttttga gtaagggata aacagaacct ttctagaaat atctattgaa
    58141 gagcaaatta tcttcaataa aagagcaata cagttggaaa agaaaaagcg atccgaacga
    58201 caggccaaaa cctatcgttc ggatcactat ttttctagaa ctgcattcaa gagactatga
    58261 caaaaagcat gccaaggcct tttttcaaat aacagtgttc catcattcat tactttgaat
    58321 tgcttttagc acacttcttg aaagcgggcc aacgatgatc aactgcaacg gtaaagcggc
    58381 aatcacattc attttccaag cattcatata agcagagaat aattgatcag gtattccttg
    58441 ttccatgatc acaccgaata aagacataca tgtcaccatt cctaaaacca tcaagcatga
    58501 aatagttaag atcatttgga ccttgctttc tggatttact ggtagtttaa aggcaatttt
    58561 tttcgccagc acaccgatga tgaagacatc caaaatgaaa gccaccacga atccaggcac
    58621 tagaccaatc aacaaacttg ataaatgaag atcttgatgc agccataaat tatacgcgct
    58681 catccccaag accatcaaaa aacacatcat tgttgtaaaa attattcctt cttttttatt
    58741 tgtaggcaat ctgtaccact cctttaaatt tgtcacgaaa atcatcgtag cacatttaaa
    58801 aatttctcgt aagtactttt tttattttct gcccttttat ttcataaact tcatcagccg
    58861 tacgctcgac aaagtaagaa tcatgagaag taaacaaaat cgttcccgga tatatttgca
    58921 ttagcttctc taaagcttcg atcgttgcca tatcaataaa attagtcggt tcatctaaca
    58981 gcaacacatt acttggctta gtaaaaagca aagcaatcgt cagacgggtc gcttctcccc
    59041 cacttaatgt acataacggt ttcgtcaaag tttcctgagc aaaccctaag tgattcagca
    59101 aactacggac aagcgattct gaaaactctg tttgtctcat gacaaaacga atgatggatt
    59161 cttcagatgt catttgataa cccttttgct gatatacttg aaagcttact ttcggagaga
    59221 gaaggattcc ttctccttgt ttgcggatat gttctaataa ggttgtctta cctgaaccat
    59281 tttcaccgac aatcgcgatt tttttaccta aagaaaactg aaaatcacca tttaccagta
    59341 atgttctccc acttctttcc aattggacat tttgtcccat gatgggatag cggctgtgga
    59401 gttcaagaga ttttggtact ggaaaacgga tttgtttcat ctgctgcggc ttggttgttt
    59461 ctcctatttt ttggagtctt ctctctaatg ttttcgcatt cttttggatg gctttttgta
    59521 cactgtcttt ttgtttagag gaagacaacc gatccagtct gatttgttgc tttttctttt
    59581 tactggacac tttttccaac ttttccgctt gttttttctt ttcttggatc gatttttcca
    59641 atcgtttttt ctctttcatg atcgactctg cttctcgtag ctgagtcttt ttctccaatt
    59701 ccttttgagt gagataggca ctataattcc ccgaaaactc tcggatttct ccgtctttta
    59761 cttcccagat tttcgatgct agctcatcta gaaaatagcg atcatgacta acaaaaacca
    59821 gcgtaccata ataataacga agctcttcaa tcagtcgatc aatacttttt ctatctagat
    59881 gggttgttgg ctcgtctaga agcatccctt gttcataaga agaaagaaga cgtatcaact
    59941 cgatctttcg ttcttcccct ccgctccatt tccgctctga agtttcttcc agtcttaact
    60001 ggaaatgact ggctaattcc aaatttaggt cctttgcttc agcgagatag gttaattgag
    60061 gcaggtagtt gaaggtgatc tctgtttgga ttttcccatg atcgggaaaa agttcaccgg
    60121 ctattagttt gagcaaagtc gatttaccga caccattttc tcctatgatc cctatacgat
    60181 cttgctgata gacacgaaga gaatcaatag atagttctag ctggttgcca aagctgactt
    60241 gtagatttgt tatatttact gctaaatttt ccatatgttt tacactccta tttcatgtaa
    60301 aacaacaatt atcgatacta aaaaagagca gaaaaatctg tttgcaaaaa aactgcatca
    60361 catcttttcc tactctgaac gaaataacta tgactaaatc accaaatctg tcatagcttt
    60421 cacctattct actgtcgttc gaaaaaatgg acatactcca tccatgaaat tgttgtttat
    60481 acatgtcttt tgaatttatt tagttgttat tcaaaagttc gaaacgtaat ttcatcgatg
    60541 cagtcatttt tccgacctcc ttttttatta ggttaccaca cacgattcaa aaaaagcaat
    60601 agctactgaa tagtaactga aaacctttta ttcgaacata gtgatctttt ctggtgctaa
    60661 acgaacagac tcgtaacctt cttctaccgc ttttcctatt gataaaataa taactggaac
    60721 ataacggtct ttatccaaac caaatgcctc tgccaactga tctgcttcga atccaccaat
    60781 tggatttgtt tcatagccat gatcacgcgc aactaacatg aattgcatag ccgctaagct
    60841 agaatcgact ttgactacat cgttcatttc ttctcttgaa aaactcttgt aataaggaat
    60901 gatcgcagct aattgttgat ctctgacttc ttttggcatc ttccctgatt cgtaagcttg
    60961 gttgtagatt tcttcgccat attcgtaaca ttgcatatcg ccaaaaatca tgaccattgc
    61021 agaagaagta tcattttgtc tcgtattgaa acggatcaaa ggacgcaaga tttcttttgc
    61081 ttcctcgctt tcagcgacca caaagcgcca tggtttcata ttgacagaag aaggtgcagt
    61141 cgtcgcttct ttgatcattt ccagcatttc ttcgtgtggg attttgtatg tttcatcata
    61201 tacgcgaacg gatcttctac cagtcataat tgttgaaaaa tcattttttt gttccattat
    61261 tattaacctc tttcgtttct gttgttatat tgcaaagttg ggtattcaat ttatctaaaa
    61321 gtgttagaaa ttgtttcatt tctacatcgc tgaaagccgg ggatagcacg gaacctgtat
    61381 ctttatggtc catttcacaa tgcttgattt tcttctgggc tttttctgtt agcgctacat
    61441 acacttcccg gttgttctga ggatttctct gacgcgaaac atagccattt gcttccaata
    61501 ccttcagatg acgagtaact gctccttgat caatctgcaa cttttcttgc aaactgcttt
    61561 gtagcaatgg attttcttct ttcagtctca tcatgatctc gtatctagta agacttatac
    61621 cgatattttc ttcaaagatc tggacgattt tttgatcggc taatttgatt tgatataaaa
    61681 ggtgactaat ctcttctaaa gacataattt cctcctttct aataattgac tcgtcaacga
    61741 ttgacttgtc aataatagaa taaaaaaaga aaaaagtcaa agaatcagct gtgtaataaa
    61801 aaaaacgctc tcaatagaaa agtatagaga aaagaaattt tttgtagaat gaacggaaaa
    61861 aatgataaag aaagcacaac gctaaaacca agattattga ttcttatcaa actatagaag
    61921 atagctcttc tgcagtagat aattttacac cgccaaaaat ttccgaaatc gccttttgca
    61981 agaatcccgt cgaatattag aaattggatc agtatgctta aacagtaata aaaaagttat
    62041 gacaagatgt tgtcacaacc ttttttgagt aaacggtgtt caaaaacttg catctgcggt
    62101 aataagttca accaaacaaa aaacatgaaa atctgttttt gtttggctgt tcttactact
    62161 tgatgcaggg cagctttttc actacctctt tttaataacc ataaaatcct attccttcat
    62221 atctccagcc catcggtgtt agccaatctc tttccttagc actcaaggta tagtgatggg
    62281 taccagtttt ttcatttgga ttaaatagtc gataaattgg cttggaacca ccagaataaa
    62341 aacttagacc ttcataacgc caaccgattt ttgttaaaga gttgatctca gattgtgcca
    62401 ttgtgtaatg atggtctcca ctgtaaggat tgtataaacg gtagacaggt tgtccgctgt
    62461 ttggtgcttg ccaagcaatc ccttcatatt cccagcctac agttggcaga taattttttt
    62521 ctccggcatt caatgtatat aaatgttctc ctgtatgacg gttatacaga cgatacatcg
    62581 gagaagttga agttggtgct gtatcatact gtgtcagatt ataggtttcg attacattgt
    62641 tcaatgtaga accataatta ggcgcagtgg cataacgacc tgttagccaa gccgtagcat
    62701 ctttgtaaga gttcgtattg cttttccatg caccggaata gtagtaaacg cctggctgaa
    62761 aagaagtcgt cttcaatacc gttacgttat cttgcaatga ttctttgtac gaaggatatt
    62821 ttctaaaagc ggcattcacc gtaatccatt gcccattcac atattccgaa gtaggcatcg
    62881 taacagattg gccattatag cttcctttaa tcccaaatag attgtaatta ggggcagaag
    62941 ccaaagtact gttcccccag ccactttcaa cgatggcttg agcaatcatg actgatgcat
    63001 ataagtcatt ggcacttgcc aaactttgtg cagttgaggc aatactatca atgaacgctt
    63061 gttgagaatt agcagatgag cgagcataaa aagcatagcc agtatagttt tcgaattcag
    63121 acaaatctgc ttgatgggta tgtgtcaatg tttgcccatc tgtcaattgg ttcgtgattt
    63181 cttctgcatt ctctggattg ttctgagctt ctgctgatgt actgtcttcg gtttgtgttt
    63241 gttctgttgt ttcagttgtt ggttcttcgg ttgaaattgt attttctgag acagtagctg
    63301 actgcaaagt actttctgta tcgttttcaa ttcctgtttc tgaagaactc gatgtctgag
    63361 ttgcgacagt ttctgtcgat tcttcagcta gcactgttgt gttggcaact gctagatttc
    63421 ctaaaagaat aacgctaaat aataaattct ttgctttcat cttttttctc ctttgaatta
    63481 tgaaactttt cctaaataac agtgtaacta aaaaagtaaa agcaagtcaa tgtccatatt
    63541 taaataacat ttgtttcttt ttaataaatt tgctttttct gcataaaaag acgtaaatga
    63601 agaatattct tctccattca cgccttaaat tgatggtcgt ttgaataact atttaacaag
    63661 taattctctc tggatattat ccgttatgga ttatacttcc tgtgtcgtcg gacggataat
    63721 catgttgctg acagacatcc ggtctggcgt ttctatcgca aatgctacag cagaagctat
    63781 atcttcagat gttaatcccc attctttttg cgcttcatga agttcaagag cagccttttt
    63841 gtcgctgatg gtttgatata attcagtttg aacagctcct ggagagatga tcgtagattt
    63901 gatgttattt tgccgttgct cttgacgtaa tccttccatg attgcacgga cagcaaattt
    63961 tgttccgcaa taaacggcgg aatcaggata aacaacatgt cccgccacag aatcagttga
    64021 aatgatatgc ccagattttt gtttttccat gattggaagt acggcggaaa ttccatttaa
    64081 cacacccatg atgttgatat caagcatatt ttgccattca tcacggtgac cttcaactaa
    64141 tggagcggtt ggcatgattc ctgcattatt gtacaataca tcgattcgac cgaacttttc
    64201 catcgctaaa tcgataactg cttggacttg tgcaaaatct cttacatctg ctgtttgaat
    64261 atatagctca gcttctggta aagattcttt tattgctttt aggcgatctt ctcggcgtgc
    64321 agctataact agtttagccc ctttttctgc taatagcttc gttgttgctt caccgattcc
    64381 gcttgaagcg cccatgatga caatcacttt ttctgataaa tcattcattc tattctcctc
    64441 ctgttttgaa aaatctcttg cttatactca ctatctctta tttcatctta ctgttcaaat
    64501 aatccgttcg acaggtttga cgattttggc agggattcct gcaacgatgg tattgtctgg
    64561 gacatctttc gtaacagttg cattcgctgc aacaatggca ttctcgccaa tcgttacgcc
    64621 tggcagaata gttgctcctg ctccgatcca agcatttttc ttgatgtgga ttggctttac
    64681 tgacaagcct cttcgttctt ttggatcggt tagatggttc accgtgatga ggttgacacg
    64741 aggaccaatc aatactgaat cttctaaaca gatgccacct aagtcaacga acatcacttg
    64801 acgattgata aagacatttt tccctatact aatatgtttg ccgaaatctg tataaaaagg
    64861 cagcagaaca gtcaccgtat catctacttt gtcactaatg atattttcta gccgtttgcg
    64921 gatatctgct ggttcatggt acccagtatt cagcgctgct aaaggctctt cgttttccgc
    64981 ttggatctga tggatcactt caaagagcgg atccgttact cgaatctctt tattgacgat
    65041 ttgtttttcg atgaggcttg tcattttgct ccctcctttt atccttactt tataacggac
    65101 ggcttatgat gtctaatact tataaaacat acttaactat tccttttaac tatacttagt
    65161 ggtatgcttt tttaaaaaca ggagggaaaa aatggatatt cgtatactga cgtatttttt
    65221 agcagtagct agagaaaaaa atatcagccg agcagcaaaa tctctgcata tcacgcaacc
    65281 tactctctca aaacaactga aagatttaga agaagaactt ggtgtgacac ttttcactcg
    65341 aggctcccga gagatacagt tgacggattc tggtcgatat ctttattcaa aaggaaaaga
    65401 aatcttgtcg ttggtagaaa aaacggcgaa caatttaaca aatgacgcaa tcatctctgg
    65461 agaaatctac attggcggag gcgaaactaa agcaatctct ttcatttctg atagccttca
    65521 ttcgttgatc aaggcgcatc ctgatatcca gtttcattta tatagcggga atgcagatga
    65581 tatcatagac aaactggata gcggattact tgattttgga ctcgtaatcg aaccgacaaa
    65641 caaacaaaaa taccagtcat ttcgactgcc agcccaagat cgttggggac ttttgataca
    65701 tcggaatcat cctttagcaa agcaaacagt catccatccg caggaactga aaactgttcc
    65761 tttgatcatt tcccggcaaa cgtcagtgga cagtcaacta gctgaatggc taggtttcag
    65821 catagatcag ttatcgatcg tcggaacata taacttattg tacaatgctt ctttgatggt
    65881 cgataacggg acttttggtg cattagctct agatgggatc atcaatactt atgggacgga
    65941 aaaagtattc atcccactcg atccgccatt acaagcttca ctgaatctca tttggaaaaa
    66001 agaccagcct ctttctcctg ctgctcgtaa atttttagat gtcctaacgg agaaatacag
    66061 ttaagtagag tttttttcct tcgctttcag ctttttaaca acgaataagt taagcaaaag
    66121 atccaatgat taaataacat aagtttctta atttgttcct tccttttatt tctgataatt
    66181 ccagtaaatt actataatta taaaaaaaga attgacaaaa cataactgtc tatataaaat
    66241 tgattacgtt aacaataatt cgtttcatgt gactagatca tactaggcat gaaaaatctc
    66301 tgaggaacat ggtttcccta ggaggttttt cgtgtctatt tttgtttggc cgcaatcaac
    66361 tggtctagca aacgtgaaaa ccaaatcttc aaaaagggtg cgcgcatgaa aatcaaaaag
    66421 atctttaatc aaaatgcagt attggtagat gacggtggac aggaaaaagt agctattgga
    66481 aaagggattg gatttgacag aaaaagaaat gatttgattt ttgatcgtga tgtcgaacgg
    66541 atatttgtca tggaaccaga aggtcaaatc aagttgcaaa atttacttaa tcagattgat
    66601 gaacgctttt tatttgcagc agaaacgatc attgatcatg cggaaactgt tttgatggaa
    66661 aagttgaatg aacatgtatt gatcgctttg accgaccaca ttgctttttc tgcggagaac
    66721 attaataacg gtatcgtcat ccgcaacaaa cttttaagag aaatcgaagt gctttacagt
    66781 gatgaattca gtattgctca atgggcagta gagtatctga caaaagagct tgggattcca
    66841 tatacttatg acgaagcagg atatatcgca atccatattc acagcggacg aagcggtcgg
    66901 aacaataacc atcgcagtat ccgtgaagtg acgattgtat ctgacatcat ccatttagtg
    66961 gaatcagaat tggaaacaga tatccattca gaaccatttt cactgaatta ttcaagactg
    67021 gtgaatcatt tacgactgct tctacaacga acccatgcgc aacaatatgc tgttttagac
    67081 acggaaatcg tagaaatggt caaacgaaaa tacccagaaa gctataagat ttctaaaaaa
    67141 atccgtgtgt tgctgaccaa agaataccag ctcgcgatca cgaaagaaga actgggttac
    67201 ttggccatcc atatcgagcg tttaagaagc gcaatcgtac aaacaaatgt taacaatcat
    67261 gaggaggaaa aaaattgaaa gcatatctac aacgaatggg acgttccctt caacttccag
    67321 ttgcggtctt acctgctgct gctttactag tagggatcgg aaactggtgg gcatcttata
    67381 gtaacgacat tgtcgctcat tttttacaag ctggcggtaa cgcagtttta ggtcaattac
    67441 ctttgctgtt tgccgttggg ttagcactag ggatgtcagt ggacaaatca ggtgctgctg
    67501 ccttggcagg tttggtcgca tttgaagttc cagtaaatgt tttaaaaact gattcagtag
    67561 ctactttatt gaatatcaaa ccagaatcag ttgatccatc ttttgctcag atcagtaacg
    67621 tatttattgg tattattgcc ggattgattg cggcagctct ttataatcgt ttccacgaag
    67681 caaaattacc gatggcttta tctttcttta gcggcaagcg tctagtgcca atcttggctg
    67741 cctttgtcat gttgctgatc agtgcagtgc tactgctcgt atggcctccg gtttactctg
    67801 ctttagtttc ttttgctaaa ttcattttga gtttaggctg gggtggtgca ggtctttatg
    67861 gcttcttcaa ccgtttactg atccctacag gattgcatca agcaatgaac tccgtgttct
    67921 ggtttgacgt agcaggaatc aatgatattg gtaaattttt ggcttctgaa ggaacaaaag
    67981 gcgtaacagg aatgtatcaa gctggatttt tcccaatcat gatgttcggg cttccagcag
    68041 gagcatatgc tatttacaga aatgcacgtc cagaaaacaa agcaaaaaca gcttccttga
    68101 tgttagcagc agcttttgct tcattcttta ctggtgtaac agaaccactt gaattctcat
    68161 ttatgttcgt tgcttggcca ttatatgtct tgcatgcaat tttcactggg atttctttag
    68221 cagtgagtgc attcttccat tggacagcag gatttgcctt tagtgccgga tttgtcgatt
    68281 acttcctatc tttgaaaaat ccgattgcga atcagccatt gatgttgatc gtccaaggat
    68341 tagtaacagc agtgatttac tactttggct ttaactttgc tatcaaaaaa ttccatctga
    68401 tgactcctgg ccgcgaggaa gcagatcttt cagatgacga tactgcaacg actaattcag
    68461 acaataaata tgcggcacaa gcaagcaaaa tctacgctgc attaggcgga gccgacaatg
    68521 taacttcgat cgataactgt actacacgcc ttcgtttgca agtcaaagat acaggcctga
    68581 tcgatcaaaa taaaatcaaa gccactggcg tacctggtat gaagatcatc gacggaaaaa
    68641 atgcgcaagt catcgtaggt acagaagtac aattcgttgc agatgagatg gcaaaactac
    68701 atggtggtgc tgcaacacga cctgcacaaa caaacaccgt ggttaaaaca gaaacatttg
    68761 ccggtggaac gatcgaacaa gatatttacg ctatcgcaaa tgggaaactg attccaatca
    68821 ctgaagtttc agatgatgta ttcgcggaaa aaatgatggg tgacggctat gctgtattac
    68881 cagaaaatgg agaaatcttc tctccaatcg ctggtacgat cacaaacatt ttcccaacga
    68941 aacatgctgt cggaatccaa acagatgctg gtatcgaagt cttgcttcat atgggcatca
    69001 acactgttga cttaaaaggc gaaccattca ctttatatgt aaaagaaggt caaaaagtcg
    69061 caagaggtca attgattgct ttagttgatc ttgccgctat tcaatctgca ggcaaaaaca
    69121 cggatatggt cgttgtattc acaaatggcg acaaagtcca aagcttagaa atcgaacccg
    69181 cacgtgatgt aaaagcaaat gataaaatcg gctctgtttc caacaaagcc tgagccatcg
    69241 tcttccccag acttttttat cgataaacaa acaggagctg agacaaaaat cttgtctcag
    69301 ctcctttttc cgaataaaaa cggcgttctc ctgccaactc cgcggtaata agtccagcca
    69361 aacgtaacat atgaagagct attttttatt tgactcttct tattacttga tgcagaacag
    69421 cttttttaca acctctgata aacggtgttc aaaaaccagt ttatcggtat gaagtcaaat
    69481 ttatgcaaaa atgtggaaag ccattttcac aaaatttttc ttcatactca aaactaatcg
    69541 gctttttcac aacctcttta tacattattc ctgatccaat atttctttta atgctgcttc
    69601 aagagaagtt aatggttttc ccaatacttt ttctaaatca tcagaggaaa tagctaactg
    69661 atccttttta atatcatgtt ggatcgctaa aaacatgtca gctgcttctt gagggaatcc
    69721 ggcttctttt agattttcga caaacgctgc atcacttgct tcaataattt ctaacttttt
    69781 acctgtcgca cgctctaatg ccttggctaa ttcttcatag tcagttagat tgccggataa
    69841 ttctaagatt tctggagagt cactttctac tagtactttt gctgcagctt ctgcatattc
    69901 acgtttcaat gcccaacctg tttttccatt tcctgcagca taaacgaatt gaccagtttt
    69961 taaagcctgc ccaatgatag gtaattcatt ctctacatac caattattcc gtaaaaatgt
    70021 atgggaaata cctgaatcct taatcatttt ttcagtgaat tggtgatctg gcgctaatac
    70081 actgatcgag tgatcggcat ctgcaaaact tgtataagcg atataagata ccccagctgc
    70141 ttgtgctgcc ttcactacat tcttatgttc ggcttgacga tttcctggaa cgccagagac
    70201 aaaaagcaat cgatctattc ctttcaacgc ttctttcatt gagtctaaat catcataatc
    70261 accaatacgt atattaaatc cagcttcttt cagtgcttct cctttttctt cactacgtac
    70321 tagtacatag atttcatttt gtggaaccat cttttttaag aattccagcg cataaccacc
    70381 aaaacctcca gttgctcctg ttactaaata atgcatattt gccactccct attcttttta
    70441 tttgttaaga tttacagctg tacatttata aattacacct ttatcataaa gagtgtggta
    70501 agataagtca agcaaaaaaa atgaaaggat gacaactgat gaagtattct ctgcaattta
    70561 gtgatgcaat ccatgtactt gcctatattg aaatgtttca agatacccga tcactttcta
    70621 gtgagatgat tgccagtagt atcgaaacaa atgcagcaaa tgtacgtaaa atcatgagtc
    70681 agctcaaaaa atccggtttg atctttacaa aaaccggaaa accgcagcct tcccttgcca
    70741 aaagtccaga tcagatttct ttactagatg tctatcgcag catcgaaggt aatacacagc
    70801 ttatccaagt cgatccaaaa acaaatccaa attgcgtcgt tggtgcgaat atccaagaag
    70861 ctttagaaga aagctacgct cggctgcaga aaaaggcaga agaagaaatg gctaagatca
    70921 cacttgatag tctgatacat aaaatagccg ttttggaaaa agaaaaaaga ccagaaaaca
    70981 caggcctcgt agagagattt ttataattct ctctactgtg cctgcatttt tctgatcgga
    71041 atcgtctaca taaattcttt tattctccgg gggacaaaag ccttgtcatg cccctttttc
    71101 ctctctaatg ctcaggtcaa agcgctgaag tcatgacgtc ttgataaacg gtgttcaagt
    71161 agctgaccct cggcattagt tctgaaaaat gcaaaatatg gaaagctatt tcccattttt
    71221 cctctctaat gctcaggtca aagcgctact ttcacgacct ctatattatc atggtttatt
    71281 gactaccggt atccagattt ctcccatata tttcccgttg gtaactccca tttcgactac
    71341 agcatttgga cccccaacat aggcgtaatc ttctacttct cttaaaacat gcccaaaggc
    71401 cgtattcgtc aaatcatttc ttaattcttc aggcgattct gctaaagcga ttacaacaag
    71461 atacgttcct tttgggaatg ggatgattcg ggtagtgttt ggaacatcct gactcgtttg
    71521 aacaccgaca tagtacatca ttttcccgtt atagggttcg ttgactgtaa agaaataatg
    71581 attttcagca gcttgtttca atgcatctat tgttccatta ccgataactt tttgctcaaa
    71641 tgcttgtttc tctgcagcca aaccgacaaa atccgtattg ctactcttca attccacacc
    71701 aatacccgtc accgtaaaat ctggtttttc ttcgatccta tactttgcca tttctcccac
    71761 cctttctatt atattaagtt catcttacat aaaattaaag ggaaatgaca cgattacgct
    71821 cttttttcta gggatatttt tattttttaa ctattttttt gctttcactg caatccaaat
    71881 ctcactgtaa acattactga aatcctctcc ccattttgta aaagagatct cgggagcttc
    71941 gactaactca taatctgaag aaggaagcca ttctgcatag attttccccc aagtatcttg
    72001 taaagtttgg ggaaatggcc ctttattagg aaaaatggcc caagtgtgtt cttctatagc
    72061 aagcatttct aaatcagcgt acggattttc ttttgtcgta gcaaagccaa tcatgtgcat
    72121 caaactgccc ttttcttcca agcgggctcc atcaaatcca taagaaacgt tgattacttg
    72181 atttggatat aaatctccta acttatgcat ctcttccctt tgagtctccg tgatcgtctg
    72241 cgctaacttt tgtatttctt gattgactcc ttcaaatttg acaggaactt ttttcgccac
    72301 acctgccaca taaaactgtt ctttcttttc gatacgaaat tccatcgatt ttcctcctct
    72361 gacatcaata aaaaatgtca agcgtggaaa agatttttgt atgccgtatc tgctgatttc
    72421 tgaaggcaga tatcctgacc aatcacgaaa agctcttgaa aaaccttcga ctgtttcata
    72481 tccatatcta aacgccgttt ctgtcactct tgatccatct aggagatcgc gattagccaa
    72541 agccaatcta cggtttttga tatagtccat tagcgacatc cctgcaataa acgtaaagat
    72601 gcgttttaat tgatattcag agattcctgt attttcggct attattttat aggaaagtcc
    72661 ttctgtcaaa tgttcttcga tgtaatcgat caattggttc agatctttaa gcatatttat
    72721 cttcctcctt gcttgccttt ttatcatatt atcaaagaaa gtcaattact tgatgaatca
    72781 ggaaccaatt tagtcagatg tagaaaatgt tgtatggatc taaagaaaat tctcaagcag
    72841 cgtttcgtaa cggataattg gtattatctg cctatctatg ttttaccttt agaaaaataa
    72901 attttgggga agacacattc taagtaaaca aagcggtaaa aatatctttt attcccttag
    72961 ttaatccgct tactacctac tgtacaaagc aaattgttgt tcaaactgct gatcagaacg
    73021 ctgcttggat ttttctttgc gatacatcat acctctttta tcaattggaa caattattta
    73081 tttctaacaa aatcacttca tttttgtcag ataaataaaa
//
LOCUS       DARBFO010000007        60875 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000007 DARBFO010000000
VERSION     DARBFO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.007"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            199..2733
                     /gene="secA"
                     /locus_tag="SCK51_000580"
     CDS             199..2733
                     /gene="secA"
                     /locus_tag="SCK51_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEG1251229.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2859..3960
                     /gene="prfB"
                     /locus_tag="SCK51_000581"
     CDS             join(2859..2930,2932..3960)
                     /gene="prfB"
                     /locus_tag="SCK51_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEG1251230.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4065..4751
                     /gene="ftsE"
                     /locus_tag="SCK51_000582"
     CDS             4065..4751
                     /gene="ftsE"
                     /locus_tag="SCK51_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEG1251231.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4735..5628
                     /locus_tag="SCK51_000583"
     CDS             4735..5628
                     /locus_tag="SCK51_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1251232.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5780..7228
                     /gene="cls"
                     /locus_tag="SCK51_000584"
     CDS             5780..7228
                     /gene="cls"
                     /locus_tag="SCK51_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEG1251233.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7565..8422
                     /locus_tag="SCK51_000585"
     CDS             7565..8422
                     /locus_tag="SCK51_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEG1251234.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8537..9457
                     /gene="pstC"
                     /locus_tag="SCK51_000586"
     CDS             8537..9457
                     /gene="pstC"
                     /locus_tag="SCK51_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEG1251235.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9457..10341
                     /gene="pstA"
                     /locus_tag="SCK51_000587"
     CDS             9457..10341
                     /gene="pstA"
                     /locus_tag="SCK51_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEG1251236.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10356..11162
                     /gene="pstB"
                     /locus_tag="SCK51_000588"
     CDS             10356..11162
                     /gene="pstB"
                     /locus_tag="SCK51_000588"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEG1251237.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11178..11936
                     /locus_tag="SCK51_000589"
     CDS             11178..11936
                     /locus_tag="SCK51_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEG1251238.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11949..12626
                     /gene="phoU"
                     /locus_tag="SCK51_000590"
     CDS             11949..12626
                     /gene="phoU"
                     /locus_tag="SCK51_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEG1251239.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12864..13895
                     /locus_tag="SCK51_000591"
     CDS             12864..13895
                     /locus_tag="SCK51_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEG1251240.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            13892..14659
                     /locus_tag="SCK51_000592"
     CDS             13892..14659
                     /locus_tag="SCK51_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEG1251241.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14759..16327
                     /gene="liaX"
                     /locus_tag="SCK51_000593"
     CDS             14759..16327
                     /gene="liaX"
                     /locus_tag="SCK51_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEG1251242.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16624..16944
                     /locus_tag="SCK51_000594"
     CDS             16624..16944
                     /locus_tag="SCK51_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEG1251243.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16944..17294
                     /locus_tag="SCK51_000595"
     CDS             16944..17294
                     /locus_tag="SCK51_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEG1251244.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17461..18399
                     /locus_tag="SCK51_000596"
     CDS             17461..18399
                     /locus_tag="SCK51_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEG1251245.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18414..19244
                     /locus_tag="SCK51_000597"
     CDS             18414..19244
                     /locus_tag="SCK51_000597"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEG1251246.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19288..20313
                     /locus_tag="SCK51_000598"
     CDS             19288..20313
                     /locus_tag="SCK51_000598"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEG1251247.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20328..21266
                     /gene="galU"
                     /locus_tag="SCK51_000599"
     CDS             20328..21266
                     /gene="galU"
                     /locus_tag="SCK51_000599"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEG1251248.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21391..21831
                     /locus_tag="SCK51_000600"
     CDS             21391..21831
                     /locus_tag="SCK51_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEG1251249.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21834..22436
                     /locus_tag="SCK51_000601"
     CDS             21834..22436
                     /locus_tag="SCK51_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEG1251250.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22495..23601)
                     /locus_tag="SCK51_000602"
     CDS             complement(22495..23601)
                     /locus_tag="SCK51_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEG1251251.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23811..24812
                     /gene="ccpA"
                     /locus_tag="SCK51_000603"
     CDS             23811..24812
                     /gene="ccpA"
                     /locus_tag="SCK51_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEG1251252.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(25149..27494)
                     /locus_tag="SCK51_000604"
     CDS             complement(25149..27494)
                     /locus_tag="SCK51_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEG1251253.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            27773..28675
                     /locus_tag="SCK51_000605"
     CDS             27773..28675
                     /locus_tag="SCK51_000605"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEG1251254.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            28888..30663
                     /locus_tag="SCK51_000606"
     CDS             28888..30663
                     /locus_tag="SCK51_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEG1251255.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(30729..32315)
                     /locus_tag="SCK51_000607"
     CDS             complement(30729..32315)
                     /locus_tag="SCK51_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEG1251256.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            32566..32790
                     /locus_tag="SCK51_000608"
     CDS             32566..32790
                     /locus_tag="SCK51_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEG1251257.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            32932..34683
                     /locus_tag="SCK51_000609"
     CDS             32932..34683
                     /locus_tag="SCK51_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1251258.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            34683..36467
                     /locus_tag="SCK51_000610"
     CDS             34683..36467
                     /locus_tag="SCK51_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1251259.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            36793..37644
                     /locus_tag="SCK51_000611"
     CDS             36793..37644
                     /locus_tag="SCK51_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1251260.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            37698..39449
                     /locus_tag="SCK51_000612"
     CDS             37698..39449
                     /locus_tag="SCK51_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1251261.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            39453..39623
                     /locus_tag="SCK51_000613"
     CDS             39453..39623
                     /locus_tag="SCK51_000613"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1251262.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(39663..40565)
                     /locus_tag="SCK51_000614"
     CDS             complement(39663..40565)
                     /locus_tag="SCK51_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1251263.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            40796..42199
                     /locus_tag="SCK51_000615"
     CDS             40796..42199
                     /locus_tag="SCK51_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG1251264.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            42199..43632
                     /locus_tag="SCK51_000616"
     CDS             42199..43632
                     /locus_tag="SCK51_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG1251265.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            43777..44703
                     /gene="trxB"
                     /locus_tag="SCK51_000617"
     CDS             43777..44703
                     /gene="trxB"
                     /locus_tag="SCK51_000617"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEG1251266.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            44917..46644
                     /locus_tag="SCK51_000618"
     CDS             44917..46644
                     /locus_tag="SCK51_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEG1251267.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            46659..47009
                     /locus_tag="SCK51_000619"
     CDS             46659..47009
                     /locus_tag="SCK51_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEG1251268.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(47101..47961)
                     /locus_tag="SCK51_000620"
     CDS             complement(47101..47961)
                     /locus_tag="SCK51_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEG1251269.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            48053..48481
                     /locus_tag="SCK51_000621"
     CDS             48053..48481
                     /locus_tag="SCK51_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG1251270.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            48643..48819
                     /locus_tag="SCK51_000622"
     CDS             48643..48819
                     /locus_tag="SCK51_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEG1251271.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            48846..49292
                     /locus_tag="SCK51_000623"
     CDS             48846..49292
                     /locus_tag="SCK51_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEG1251272.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(49446..50825)
                     /gene="brnQ"
                     /locus_tag="SCK51_000624"
     CDS             complement(49446..50825)
                     /gene="brnQ"
                     /locus_tag="SCK51_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEG1251273.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            51531..52502
                     /locus_tag="SCK51_000625"
     CDS             51531..52502
                     /locus_tag="SCK51_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEG1251274.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            52526..54718
                     /locus_tag="SCK51_000626"
     CDS             52526..54718
                     /locus_tag="SCK51_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEG1251275.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            54735..55211
                     /gene="ybeY"
                     /locus_tag="SCK51_000627"
     CDS             54735..55211
                     /gene="ybeY"
                     /locus_tag="SCK51_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEG1251276.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            55195..55590
                     /locus_tag="SCK51_000628"
     CDS             55195..55590
                     /locus_tag="SCK51_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEG1251277.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            55605..56504
                     /gene="era"
                     /locus_tag="SCK51_000629"
     CDS             55605..56504
                     /gene="era"
                     /locus_tag="SCK51_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEG1251278.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            56647..57459
                     /gene="recO"
                     /locus_tag="SCK51_000630"
     CDS             56647..57459
                     /gene="recO"
                     /locus_tag="SCK51_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEG1251279.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            57843..58760
                     /gene="glyQ"
                     /locus_tag="SCK51_000631"
     CDS             57843..58760
                     /gene="glyQ"
                     /locus_tag="SCK51_000631"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEG1251280.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            58762..60837
                     /locus_tag="SCK51_000632"
     CDS             58762..60837
                     /locus_tag="SCK51_000632"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEG1251281.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        19863 a        10760 c        13027 g        17225 t
ORIGIN      
        1 aaaacttgca gcagctactg acagctgccg caagtttttt tgagtttcct cctcttttag
       61 atgaaatatg aataaattat gaagttctta ctttaatacg cttaagatat ttcaattatc
      121 tataactaat gataaaatga aaagtaaagt ctacatagtc tggataatac acagaaggaa
      181 aaggagatta cacaacgcat ggccaatttg attcgttcac ttatcgaaaa cgataagaaa
      241 gaactaaaac gtttagatag cattgcaaaa aaagttgaag catacgctga ccaaatggca
      301 gcattaactg atgaacaatt acaggcaaaa actgctgaat tcaagggcag ataccaaaaa
      361 ggagaaacat tagatcaatt acttccggaa gcttttgcag tcgtccgaga agcagcaaaa
      421 cgtgtattgg ggttatatcc acaccacgta caattgatgg gtggaatcgt gcttcacgat
      481 ggaaatatcc ctgaaatgcg tactggtgaa ggtaaaacct tgaccgcaac gatgccagta
      541 tatctaaatg ctctgactgg cgaaggggta cacgtagtta ccgtcaatga atatttagca
      601 acccgtgact cgacggaaat gggcgaactt tataatttct taggattaac agtggggttg
      661 aatatcaact caaaatcttc ggatgaaaaa cgcgaggctt ataattgtga tatcacttat
      721 agtacgaata atgaattagg atttgactac ttgagagaca atatggtcgt ttaccgccat
      781 caaatggtac aacgtccgct gaattatgcc attgtcgatg aagttgactc aatcttgatt
      841 gatgaagcga gaacgccgct gatcatttca gggcaagcag aaaaatccac agctttatat
      901 acaagagcag acaacttcgt gaaacgtttg aaagaagaag aagattataa aatcgatgta
      961 caatcaaaaa caatcagttt aaccgaagca gggatcgaaa aagcagaaga aacattcggt
     1021 ttggataact tatacgacat tgaaaatact gcattgaccc atcacttgga tcaagcattg
     1081 agagccaact atatcatgct tttagatatc gattatgttg tccaagacgg aaaagtactg
     1141 attgttgacc agttcactgg tcggatcatg gatggacgcc gttattcaga tggacttcac
     1201 caagcaatcg aagctaaaga aggcgtagag atcgaagacg aaacgaaaac catggcgacg
     1261 atcactttcc agaactattt ccgtatgtac aagaaacttg cgggaatgac tggtacagct
     1321 aaaacagaag aagaagaatt cagagaaatt tataacatcc aagtatacca gattccgaca
     1381 aatcgtccgg ttatccgtga tgatcgtcca gatttgcttt acccaacatt acaaagcaag
     1441 ttccacgctg tcgtgcaaga tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc
     1501 ggaactgttg cggttgaaac atctgagtta ttatcagata tgctgaatca agaacgtatt
     1561 cctcatgaag tgctgaatgc gaaaaatcac tttaaagaag cagagatcat catgaatgct
     1621 ggtcaaaaag gtgctgtgac gattgctacc aacatggctg gtcggggaac agatatcaag
     1681 ttaggacttg gggtaagaga acttggtggt ctagcagtta tcgggacaga acgacatgaa
     1741 tctcgtcgta tcgataacca gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg
     1801 tcacagttct acttgtcatt ggaagatgat ttgatgaaac gattcggttc agaacgaatc
     1861 aaatctttct tagatcggat gaagatcggt gaggaagatg ctgttatcca aagcaagatg
     1921 ctgacgaaac aagtagaatc tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt
     1981 aaaaacgtct tacaatacga tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag
     2041 cgacaagaag taatcatgga agaaaaaagc ttatccaagc cactaatgaa catggtcaaa
     2101 cggacaatca gtcgtgtagt agatgcgcat acacaattag agaaagaaaa ttggaatttg
     2161 gaaagtatca tcgattttgc cggcagtact cttgtccatg aagatagtat tactcttgca
     2221 gatatcgaag gaaaaacagc tcaggagatc aaagagtatc taaacgaacg agcacaagaa
     2281 gtatttaata ccaaagcggc tcaactaaat ggtccagagc aactgttgga atttgaaaaa
     2341 gtcgtgatct tgcgtgttgt ggactctaaa tggacagacc atattgatgc aatggatcaa
     2401 ttacgtcagt caatcggctt acgtgcttat ggacagaaca atccattagt cgaatatcaa
     2461 acagaaggct ataagatgtt tgaagatatg gtaggcgcca tcgagtatga agtcactcga
     2521 atctttatga aagctgagat ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca
     2581 gcttctcact cagaagacgg agatgccaaa gaaaatagta atactagcgc gaaaaaacag
     2641 ccaatccatg tgaacaaggt cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa
     2701 tataagaact gtcatggaaa aaatgcaaac taatttctga cagcaggatt ggtgagacaa
     2761 attctttttt taagaattcg tctcaccttc tttctgttat ttgctgattc tgttagacta
     2821 gatataatga aaaaagtata gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa
     2881 atctcctgac agaaatgcag gaaaaggtca caagcttcag gaggtctctt tgacttagac
     2941 cagctggaag aagacattgc ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat
     3001 gatagcgaag ctgctcaaaa agtgatcagt gaaaacaatg caaataaaga aacttatgat
     3061 caattccatt ctcttgctga agaactggaa gaaatcgaat tgatggtgga gatgttgcag
     3121 gaagaacctg atgcagccat gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa
     3181 aaaatgaaga catatgaatt atctatgttg ttagatggtc cttatgatcg aaacaacgcc
     3241 atcgttgaat tgcatccagg ggctggcgga acggagtccc aagattgggg cagtatgctt
     3301 ttacgtatgt atacacgctg ggcagagcag catgggtatc aagtagagac actagattat
     3361 caagcaggtg atgaagcagg aatcaagagt gtgaccctac tgatcaaagg acacaacgca
     3421 tttggttatc tgaaatcaga aaaaggtgtt catcgccttg tacgtatctc gccttttgac
     3481 tcagcgaaac gacgtcatac ttctttttgt tcggtagatg tgatgccaga gcttgatgaa
     3541 acgatcgata tagaaatcaa tccagatgat ttgaaaatcg atacgtaccg agcaagtggt
     3601 gcaggtggtc agcacatcaa taaaacagaa tctgctgtac ggatcactca cttgcctaca
     3661 ggaacagtag tagctagtca ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg
     3721 gggatgttaa aagctaagct ttatcaatta gaagtggaaa agaaagaaca agaagcagct
     3781 gcacttcgag gagaacaaaa agaaatcggc tggggttcac agatccgttc ttatgttttc
     3841 catccatatt ctatggtaaa agaccatcgg acaaacgtag agacaggaaa tgtccaagca
     3901 gttatggacg gtgaccttga tttatttatt gacggctatt taaaaatgca tctacaataa
     3961 gaacccattg ttaaattgaa ataaaattgt aagattttgc aagcatgttg aaacattgcc
     4021 atgctataat agctaaagac tgaaaaagat atggagagaa cgccatgatt gaaatgaagg
     4081 atgtaatgaa gaagtactca aatggtacga ccgctatccg caatctctca attgtcatcg
     4141 aacaaggaga gtttgtctat gtggttggtc cgtctggagc cggtaaatct acattcatca
     4201 aattgatgta tcgagaagaa aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc
     4261 taatagcaat caaaaaccgc gaagttcctt atctacgcag agaaatcggc gtggtgttcc
     4321 aagactataa attattacca agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag
     4381 ttatcggcag aaaacctcgg gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg
     4441 gtttgaaaca caaagttcga gtctttccta gcgaattatc cggtggggaa cagcaacgtg
     4501 tctctattgc tcgggcaata gtgaatacgc ctaaagtact tatcgcggat gaaccgacag
     4561 gaaatctgga tccagaaaac tcatgggaaa tcatgaaatt attagatcgg atcaatgccc
     4621 aaggaactac tgtcgtgatg gctacacata acagtacgat cgtaaatacg attcggcacc
     4681 gagtaattgc tatcgaaaac ggccgcatta ttcgagatca agcggaaggg gaatatggat
     4741 acgatgatta gaaccttttt ccgccattta ttagaaagta tcaagagttt gaaacgtaac
     4801 ggctggatga cagttgcttc agtcagtgcg gtaacgatca cattgacttt agtcggtgta
     4861 ttcatggcag ttatcatgaa cgcaactaaa ttagctcaag atatagaagg aaacgttgat
     4921 gtctctgtat tcgtagatat cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa
     4981 ttgaatgact tagatcatgt taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa
     5041 aaaatccaaa acgaaatggg tgattcctgg aaattgtttg aaggtgacaa taacccgtta
     5101 tatgatgtgt atgtagtaag tgctactgat ccttcttata caaaacaagt agcagaagaa
     5161 gcagcagatt tgaagaatgt ttctcgttct gattacggtg gagcttcctc agatcggatt
     5221 ttccaaatcg ctggtgcagt tcggacatgg ggattagttg cagcggcatt gctgctgttc
     5281 gtagcgatgt ttctcatttc gaacacgatt cggatcacga ttttgtctag acagcgtgaa
     5341 attcaaatca tgcgtttagt cggtgcaaaa aatggatata ttcgctggcc gtttttcctt
     5401 gaaggtggct ggatcggtct gatcggtgcg attcttccga ttttgatcat cacatttggt
     5461 tatgcatggt ttttccaatt gatcaatcca tcattattac gttcccatta ttcattgatt
     5521 catccacaaa atatcgtttg gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt
     5581 ggttcacttg gctcgatcat ttctatgaga cgtttcttga aattctaata ccacaatata
     5641 aataaaatga gaaagttgga cagtttcgtt ccagctttct ttttttattg gctaggagaa
     5701 tagaaggaag cggctttttt ccctttactt taaaaaacaa cttgggtatt cttaagttgg
     5761 tagaagaaga ggtgaagccg tgaaaattat cgttgacaat ttttttacga tccttttgat
     5821 tatgaatatt ttgctttcct ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac
     5881 atgggcatgg gtgctcgttt tgatgttcat tccagttgta ggatttattt tatatatttt
     5941 tttaggaaga gggatttcca aagagaaaat ctttgatttg aagatccaag ggaaaatcgg
     6001 taagaacctg gaaattgaag aagacaaaca ggcattgatg cgagggcttt atcctcatcc
     6061 gcctactgga caagtagatg tgaaacaatt gatctatatg ctcacggtat ttgaaagtac
     6121 gctctatacg actaaaaatg aaatcacttt atttacagat ggtcgtgaaa aattcgatgc
     6181 gctgattgaa gatatcaagc aggcagaaga ccatatccac ttccagtatt atatttatcg
     6241 cagtgatgca ttaggtgaag aagttcgaga tgcattgaca gaggcggcaa gacgaggggt
     6301 aaaagtcaga gtccttcttg atgcatgggg ttccacacaa gtttcttcca gcttttttca
     6361 gaatttgaaa aaagcaggcg gagaagtcgc atttttcttt cctctatttg ttccgtatat
     6421 taatcctcgg atcaattatc gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg
     6481 atatacaggt gggttcaatg ttggaaatga atatctagga ttggtaaaga aatttggtta
     6541 ttggcgagac aatcatttgc gtatttacgg agaagcggtg tataccttgc aaaaccgttt
     6601 tttgatggac tggaattctc agcatacaaa agaagtaggc tattctccca aattttttcc
     6661 gtccattcac tctactggtg atatagctgt tcaaatcgtg acaagcggac cagataccga
     6721 acatgaacaa atcaaaatga cttatttgaa aatgatttcg ttagccaaac gggagatatt
     6781 gatccaaacg ccttattaca taccggatgg atcgattcat gaagcactga agttggctct
     6841 tctttcaggc gtaaaagttc atatccaaat acctaacaaa ccagatcatc ttctcgttta
     6901 ctgggcaacg tattctttcg ctgcagagct aatcgagtat ggagcaagga tcgaaacata
     6961 tgagaatgga tttattcatg caaagacgat gattatcgat ggtggcattg tttcagttgg
     7021 ctcagcaaat atcgatgttc gttcatttcg cttggacttt gaggtcaata cactggttta
     7081 tgatgaacga atggcttcac gtgtaagaaa agcttttttt gaagactcga aaatttctac
     7141 gcatctcaca aaagaaatgt atgaaaaccg aggaataatc atcaagatga aagaaggatt
     7201 agcaagattg atttcgccgc tcctttaaaa cataaaaagg acatgagaac catcaacaga
     7261 tggagatctc gtgtcctttt ctaacggggc catttctgag agtacgtcag aaatggagaa
     7321 agcgtctcgc atgagcgaaa ttaactttgg ggtatctctt gttttttggg gaaagcaaga
     7381 aataagctaa tatatgctat taaaagagac acatttcaaa gccagtatag catagaaact
     7441 tttaatgaga tagttttttt tacaattatg agaatttatt ttgttattta atgattttga
     7501 ttcgttttca tcaagtaatg aaaatgataa aataaacata ctttaggatt aaaggagatt
     7561 tctaatgaaa aaacttaaat tattcttacc aataatagga ttagctattc ttttatcagg
     7621 ctgttcaaag tggattgatc gaggagaatc cattacggct gttggctctt ctgctttaca
     7681 gcctttagta gaaacagttg ctgaaactta ccagacagaa aatcctggaa aattcatcaa
     7741 tgtacaaggg ggcggaagcg gaactggttt gtctcaagta cagtcaggag cagtcgacat
     7801 cggaaactct gatttgtttg cggaagaaaa atcggggatc gatgcctcag cattagtcga
     7861 tcatcgagta gcagttgttg ggatcactcc tattgtgaac aaggatattg gcgtaaaaga
     7921 catcagtatg gaaaacctga aaaaactatt tacaggcgag atcaaaaact ggagtgaagt
     7981 cggtggaaaa gatcagccag tagttatatt gaaccgcgct tcaggaagcg gaacccgtag
     8041 cacgtttgaa aaatgggtat tggacggaga aacagctgtt caggcacaag aacaagattc
     8101 aagcggaatg gtgcgccaga tcgttgccga cacacccggt gcaatcagct atgttgcttt
     8161 ttcttatgtt tccgatgatg tagatacgtt gaatgttaac ggtgtagaac cgacagatga
     8221 aaatgtgaca acgaacaaat ggacgatttg ggcatatgag catatgtaca caagagatca
     8281 tccaaaaaaa ctgacaaaag aatttttaga ctatatctta tctgatgaag tacaagataa
     8341 catcgttgga gaactaggtt atatccctgt ttctcatatg aaagtcgaac gtgattggca
     8401 aggaaatatc gttaaagagt aatctttaca caaaatttac aaacttagct aaacttttac
     8461 atgttattaa aaccaaattg atgttcaatt tgtacactga gtgagtatgt taaacctgat
     8521 taagggggag tcatctgtgg aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc
     8581 aaaaatggaa cagcgtggaa aattcatcag ttttttatgt atcgcgctga tcgtgttggt
     8641 tgtattatct attttttatt ttgtagctag taaaggattg gccacctttt ttactgataa
     8701 gatcaatgta tttgattttc tgtttggtac agattggaat ccaagccaaa tcggagcaaa
     8761 tgggaaacca atggtagggg cattgcctat gatcacaggt tcgtttattg tgaccttttt
     8821 atctgctatc gttgccacgc catttgccat tggtgcagca gtattcatga cggagatctc
     8881 tcctaaaaac ggaacgaaga tcttacagcc ggtcatcgag ctgcttgtag gtattccttc
     8941 tgtcgtttat ggttttatcg gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg
     9001 cgggactggg tttggtatct tagctggtac atttgtgttg ttcgttatga tcttgccgac
     9061 tgtcacaacg atgactgttg atgcattaaa ggcagtgcca cggcactatc gggaagcttc
     9121 attagcgctt ggtgccacaa gatggcaaac aatctacaaa gtcgtgctac gagcagccgt
     9181 gcccgggatt ttaacagcag tcgtttttgg tatggctcgt gcgtttggtg aagccttagc
     9241 tatccaaatg gtcatcggta atgcagcatt gatgccaaca ggtcttacta ctcctgcttc
     9301 tacattgacg tccatcttaa caatgggaat cggaaataca atcatgggaa cagttgaaaa
     9361 taatgtgtta tggtcattag cgctgatctt gctattgatg tcattattct ttaatatcgt
     9421 gatccgtatg attgggaaga aaggagagct gaaataatga acgcaaaacg ctcagataaa
     9481 attgctacag gtgttttata tatagtttcg gggatcatcg tattgatttt agcagcttta
     9541 ctactttata tcttagttcg gggaataccg cacatttcat ggtccttctt gaccgaacct
     9601 tccaaagcct accaagaagg cggtggaatc gggatccagc tatttaactc gatttatttg
     9661 cttttgatca cgatgatcat cagttttcct atttctttag gtgcgggtat ctatctttca
     9721 gaatatgctg gaaaaaactg gctaacagat gtgattcgca cgtcgattga aatcttgagc
     9781 tcgttgcctt ctgtcgttgt cggtttattc ggtttcttgg ttttcgttat tcaattccag
     9841 tatggtttct cgattatttc tggtgcattg gctttgactg tattcaactt gcctcttttg
     9901 actagaacag ttgaggagtc tttacaagcg gtccattaca cacaacgaga agcaggattg
     9961 gccttaggtt tgtcacgctg ggaaacagtg ataaaagtgg tggttccaga agcgacacca
    10021 gggattttga ctggtgtgat cctaagttct ggaagaattt ttggtgaagc agctgcattg
    10081 atctatacgg ctggacaaag tgcacctgcg ttagatttta cgaactggaa tccattaagt
    10141 gtttcaagcc cgatcagcat tttccgtcaa gctgaaacat tagctgtcca tatttggaag
    10201 atcaatacag aaggcacgat gcctgatggt gtttctgttt cggctggcgc ttctgccgtg
    10261 ttgattattg ctgttttact gttcaacttc ggcgctcgtg cgattggcaa acgattatac
    10321 cgaaaaatga cttcagccta aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa
    10381 caaatgtcat caccatgcca aaagagaaag aagtagcact atatacggat gatctacatg
    10441 tctggtatgg cgacaatgaa gcaatcaaag gagtggatct ggaatttgaa aaaaataaga
    10501 tcactgcact gattggtcct tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc
    10561 gaatgaatga tgggatcgct aatacgaaaa tcactgggaa aatcatgtat caaggcgtag
    10621 atattaatgc gcctcaagtg gatgtttatg agatgcgcaa acggattggc atggtttttc
    10681 aacgtcctaa tccgttcagc aagtcgattt atgacaatat tacatttgct ttgaaacagc
    10741 acggcgaaaa aaacaaaaag aaattagatg aaatcgttga aacaagcttg aaacaagctg
    10801 ccttgtggga ccaagtaaaa gacagcttgg acaaaagtgc cttggcttta tctggcgggc
    10861 aacagcaacg tttatgtatt gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag
    10921 acgagccggc aagtgctttg gaccctattt cgacaggaac ggttgaagaa acacttgtaa
    10981 acttgaaaga agactatacg atcatcatcg tgacgcataa catgcaacaa gcagctcgta
    11041 tcagcgacta tactgccttt ttctatttag gaaaagtaat ggaatatgac aaaactaaca
    11101 agatatttac cagaccaaaa attcaggcaa ccgaagatta tgtgtccggt cattttggtt
    11161 aagacggagg cctcaacatg acaaaagaga ttatttcatc taaagatctt catctgtatt
    11221 atggaaagaa agaagcacta aaagggatcg atctaagctt caatcaaggg gaaatcactg
    11281 cgatgatcgg tccttctggt tgcggaaaat ccacttatct gcgttctttg aaccggatga
    11341 atgacctgat tccgaatgta acgatcaccg gaagtgtttt atataaagac aaagacatct
    11401 atggtccaaa aacagatgtt gttgaactaa gaaaagaaat cggcatggta ttccagcagc
    11461 caaacccatt tcctttttct atctatgaaa atgtcatcta tggattgaaa ttaaaagggg
    11521 aaaaagataa aaaagtcctt gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt
    11581 gggatgatgt gaaagacaaa ttgcataaaa gtgctttatc tttatccggt gggcagcagc
    11641 agcgtgtatg tatcgcacgt gttttggcag ttaatccgga aatcatctta ttggatgaac
    11701 caacaagtgc gcttgatcct gtttctactg ggaaaatcga aagtatgctg ttagaattaa
    11761 gagagcggta tacgatgatc atcgtgaccc ataatatgtc tcaagcttca cgtatttcag
    11821 ataagacggc attcttttta gatggtcact tgatcgaata caatgatacg aagaagatat
    11881 tcatgaatcc agaaaaacaa gagacagaag attatatttc aggtcgattt ggataaggag
    11941 gaaacggaat gctacgtaca caatttgaag aagaattgct gaatctccat aatcagttct
    12001 acgaaatggg gatgatggtg agcagcgccg tgcacaagtc agttcgtgca tacatcaaac
    12061 acgataaaat cttagcccaa gaagttatcg aaaacgatat aaatatcaat aacatggaaa
    12121 cacgcttgga gaagaaaagt tttgaaatga tcgcattgca acagccagtt acaactgatt
    12181 tgcgtatgat cattacggtc atgaaagcta gctcggattt agaacgtatg gccgatcatg
    12241 cggtgtctgt tgctaaatcc acgattcgcg taaaagggca gacgagaata ccggaaatcg
    12301 aaaaagagat ctccgatatg tccgattatg tgaaaaaaat ggtggacaac gtcctagttg
    12361 cttatgtcaa aacagacgaa aaagacgcac gtacgatcgc caatatggat caaagagtca
    12421 atgactattt caatcgaatc tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga
    12481 cagtcatcag tggaacagac tacttgaatg ttgccagtta tctagaacgg atcggagact
    12541 atgtgacaaa catctgtgaa tggatcgtct acttggctac agggaaaatc actgagttaa
    12601 atacgaacca caatgagatt ttctaaactg aagctgtgat tatgccgttt gtttaggaaa
    12661 agaggttgtg aaatcagcgg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc
    12721 aaacaaaaaa tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc
    12781 ttttgaacac cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca
    12841 tcatttcata gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta
    12901 tttgtgtgct tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca
    12961 tcgagataga aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga
    13021 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
    13081 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
    13141 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
    13201 ccatgttgct tattggtgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
    13261 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
    13321 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
    13381 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
    13441 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
    13501 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
    13561 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
    13621 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
    13681 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
    13741 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
    13801 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
    13861 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
    13921 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
    13981 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
    14041 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
    14101 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
    14161 cctactattg cttcctatca ctgatttttt agcaatccaa ggttttacat cggtagaact
    14221 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
    14281 gttgcttctg attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg
    14341 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
    14401 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
    14461 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
    14521 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc
    14581 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
    14641 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
    14701 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
    14761 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
    14821 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc
    14881 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
    14941 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga
    15001 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
    15061 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
    15121 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
    15181 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
    15241 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
    15301 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
    15361 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
    15421 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
    15481 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
    15541 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
    15601 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
    15661 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
    15721 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
    15781 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
    15841 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
    15901 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
    15961 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
    16021 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
    16081 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
    16141 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
    16201 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
    16261 tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
    16321 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
    16381 atagcttccc ctattttaca aaattgtgcc ttattgccga tagtctatct cttgaacacc
    16441 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
    16501 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
    16561 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
    16621 gacatgatga aaaaaaagtt gacaaaatct aataaaaatg ttgttttgac agggacttta
    16681 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
    16741 ctgagcttag ttgcttttgg aagtccaatt attttataca ttttattagc tttgattatc
    16801 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
    16861 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16921 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
    16981 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    17041 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
    17101 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    17161 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
    17221 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata
    17281 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
    17341 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
    17401 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
    17461 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
    17521 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    17581 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    17641 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    17701 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg
    17761 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    17821 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    17881 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    17941 aaaagtgaaa cagcgttgga gttgatcaaa cgcggccatc gattgatcgc agacgatcga
    18001 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    18061 catctgattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    18121 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    18181 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    18241 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    18301 gcgatgaatt tccgcgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    18361 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    18421 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    18481 tcatcgtatc aggtatcatt atcgctatgt ggcttagcag cagagaggct gttcgggtag
    18541 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    18601 gcgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    18661 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    18721 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    18781 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    18841 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    18901 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    18961 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    19021 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    19081 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    19141 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    19201 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    19261 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    19321 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    19381 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    19441 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    19501 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    19561 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    19621 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    19681 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcaaaaca tgacatcact
    19741 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    19801 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    19861 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    19921 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    19981 ggtgccaacc cgctgacctt tatcggatta agcggaatag gcgatctagt cgttacttgc
    20041 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    20101 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg
    20161 atggaactag cccaaatgat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    20221 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa
    20281 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagttaaaa
    20341 aagcagttat tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg
    20401 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    20461 tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    20521 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    20581 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    20641 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg
    20701 tcgttatgct aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg
    20761 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    20821 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    20881 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    20941 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    21001 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    21061 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    21121 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt
    21181 taggtaaaac attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    21241 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    21301 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    21361 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    21421 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    21481 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    21541 acaaaagacg tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa
    21601 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    21661 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    21721 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    21781 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    21841 aaaaaggtgg atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt
    21901 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    21961 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    22021 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    22081 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    22141 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    22201 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    22261 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    22321 aagcaaaacc agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga
    22381 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    22441 acaaatttat agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt
    22501 ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac
    22561 atatacacag tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag
    22621 agagaagcac atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc
    22681 gtggacggtt gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata
    22741 gctgttgatc acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac
    22801 ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc
    22861 ggttggtttt tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac
    22921 gccaagatcg aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg
    22981 agggcttgca gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc
    23041 ttgttttttc aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt
    23101 cactgcttta aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt
    23161 gtctttttcc tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt
    23221 gtaagaatcc gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc
    23281 gttcttttca atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat
    23341 gatctcccaa gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt
    23401 ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat
    23461 aaaaagagct agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg
    23521 gccagggtcg ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc
    23581 attgattttt acgttattca tttggcaacc tactttctaa attaattttg tattcaataa
    23641 ttgtaccact gatttattaa aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc
    23701 atgaaaacag agataaaacg gttgcaattc ttgagattat ttgatattct attttagaaa
    23761 tgtaagacgt attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc
    23821 aaacaatcac tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac
    23881 gtgtcgtaaa tggcaatcca aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta
    23941 tcgatcgctt ggattatcgt ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa
    24001 caacagttgg tgtaatcatc cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg
    24061 gtatcgatga tgtggcaaca atgtacaaat acaatattat cttagcaaat tcagatggca
    24121 acgaccaaaa agaagtcaat gtattgaata acttactggc taagcaagtg gacggcgtga
    24181 tcttcatggg tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaactc
    24241 ctgttgtatt agctggctct attgatccag atgaacaagt aggtagcgta aacattgatt
    24301 atgtatccgc aacaaaagat gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg
    24361 cttttgtcag tggtgcgttg atcgatccca tcaatggaca aaatcgtctg aaaggctata
    24421 aagaagcatt aagcgaaaat aacctaccat atagcgaagg attgatcttt gaagcacaat
    24481 acaacttcaa agatggtcta tctctagtcg atcggatcca taatagcggt gcaacagctg
    24541 tctatgtttc agatgatgaa ttagccatcg gtattttgga tggtttatta gaccgtggcg
    24601 tgaaagtacc ggaagaattc gaaatcatca caagtaacaa ttcactactg acagaagtag
    24661 ctcgtcctcg tttaacaagt atcacacaac cattatacga tttaggggcg gtatctatgc
    24721 gcctattgac gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt
    24781 atggaatcga agaaaaagga tctacaaaat aataaaagga caaccattca gaggttgtgt
    24841 gcctgccgtt taactctgcg ttacaaggag caatttcaaa aaacagcatc tcatgtttga
    24901 tgaatattgt gactcgtgat tcggagttgg caggagaaca ccgtttattt gggaaaaggg
    24961 gttatgacaa aattttgtta taatcctttt tttgtgtctt aaaaaagaga gaaagtggtt
    25021 gaaattacat ttatcgtata taaaacaatc cgtgtataga tgctgcattt tgttcaccac
    25081 catcaaaaaa ataagggact gtgacaatac tattgtcaca atcccttatt actgtgtatt
    25141 ctattaaatc aatcatcttt ctcatctgat tctttagttg cttgattcgc tcttgcataa
    25201 gctgaagcgg tgttccagta atctgtatag ttttcatcgg taccgccaac gccaaattta
    25261 aaggcgcttt tcttcggtcc atttttcgcc catagcgatt caactgtttt tcctggtgtt
    25321 tggatggatt ttttattgac agtaactttt ccaggtgttt gaccagtaaa tccagatact
    25381 tttttcttca tcacatcaga tgataattcg aatttctcat cagtgccaaa aatgtcaggt
    25441 tttgcttggt aagcttgatt gatcagattg gcaagataat tagccgaacg tatccttgct
    25501 ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc tactgatggt aatagacgga
    25561 gtagagacaa ttagccatga atctcggtat tcattggtag aaccagtttt tccgacccaa
    25621 tctgcttttc ctagatttcc gtttaagctt gagatggcat ctttgaatgg tgtagtgatt
    25681 tttgcattga tcacactacg catcatgtca ttcatgatcg atgccgttgc ttccgaatag
    25741 atccggacag agttgctttc gtgtttataa attacatttc ctgcgttatc tgtgatggaa
    25801 tcgatgatat agccttcttc atagacgccg ccgtttgcta aagcctgaaa tccgttggtc
    25861 tgttgaagcg tcgtgacatc tactgtacct aaaggagcag attcaacgcc ccaattgtca
    25921 tttgccgggt agttcatctt tgccaagtag tgctcgtaag catattgttt cgatccaccg
    25981 gaattcaaca catcttgata taagtgataa gccgggatat tgttggacca ttctagtgac
    26041 tcacggacgg tttggaaggt gttcgatcct tcgttagtag cattgacgat tttctcgcca
    26101 gcattttctc cttcttgcca agttgttgca taatctgata cacgggattc tgatccgata
    26161 agccccatgt cgattgcagg tccataaaca agaactggtt tgattgatga gccagcttga
    26221 cgttctgtat cgaaggcatg gttattttga ttctgactgt aattccgtcc gccaacgaat
    26281 ccgtagatgc ggccagttct gttatccatc aatacatttc cggtttctac ttgactgcca
    26341 gcaccatcat ctaacatgta tccataattt tcaacgccat tttgcaatgc agcatagaca
    26401 tctttatcga ttgtcgaatg gattgtatat cctttatttt ggatcgtttg ctgtgccttt
    26461 tgataataag cgtcactgac cgatgaatca gacatatcag cgttgtcttt ttcagccaat
    26521 tgttgtgcaa tgattcttgt tgcttcattc atcacagtat aatacaagaa ttctctgtca
    26581 gtctgttctg cgatttgttg tggtagaaag tcttttgtaa ggtcataggc ttttgcttct
    26641 tcgtattgtt ctttcgtaat ttggccttca cgatacatac tgaataaaac aaaatctttt
    26701 ctggcaaggc cggcagaaag atcttctttt agcgcgcctg tgttcgtgta tggactatac
    26761 gtgatcgggc tttggggtaa tccagcgata taagcagctt gcggcagcgt cacatctttt
    26821 gcatctacac caaagattcc ttttgcagct tcctggactc cagcaatgtt ttgtccctta
    26881 ttattacgac caaatggtga gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag
    26941 tatttttcta cttgtgcaga aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca
    27001 tctgtaagga tctgctgttt gactaattgc tgtgtcaaag ttgatccacc agaagagcca
    27061 ataccagtag cttcagatac taatgctcgt aaaacagctt taggaacgta accttggtgt
    27121 ttatcgaagt attcatcctc tgtgctgata attgcagttt tcaataaagg tgaaatctta
    27181 tcagatgaaa tagtcgtgcg catcagatct gtctggattt ttgatatctc tgtattatct
    27241 gcataaacaa tctttgacgt ttctgtaatg tttcctagtt ctgtctgcag tgctttttta
    27301 gtcggcaatt gtgtgtcttc gacgagatag gcaaaatagc cagcgcctat tccaatacct
    27361 agagctgcga acatcaaaag aagaacagta ataaaaacag tcaatgattg aaaaactcta
    27421 aaaataatct taggaacaaa ccatttttct ttttttcttc tattctgttt ttttcgagta
    27481 tttttgcgtg gcaaaagggt cacctcaact aatggtttta tttcgctaat tatagcaaat
    27541 aatagaacaa aaaaacagac aaggctgttt ttggcaggaa tttctttttt aaaagagtta
    27601 aaacgtgaga aaatgatctt tattataaaa gagttcttaa gcttttcttt agagaaaatt
    27661 tcagttacta tggagaacag ggcaaagaaa aatcaatcat tttctttgta tatcatctta
    27721 ccaatatata ggaattgtta gtaacaattg cctttcaagg aggaatccat ccatgttatt
    27781 aggctcacac gtcagcatga gcggaaagaa aatgctgtta ggagcagcag aagaagcagc
    27841 cagttatgga tcttcgactt ttatgatcta tacaggcgcc cctcaaaaca ctagaagaaa
    27901 agccatcgaa gagatgaata ttgatgccgg aaaagcgtac atggcagaaa aaggtctaac
    27961 aaatatcgtc gttcatgccc cttatatcat taatttgggg aatacgatca aaccagaaat
    28021 gtttcctttt gccgtccaat ttctgagaga agagattgtt cgtgcggaag ctttaggcgc
    28081 tactcaaatt acacttcacc caggtgctca tgtaggggca ggaacagaag ccggaattgc
    28141 tagaattatt gaaggcttga atgaagtttt gacaaaagaa cagacggcac aaatcgcttt
    28201 ggagacaatg gcaggtaaag gtacggaaat cggtcgaaca tttgaggaat tagcgcaaat
    28261 catcgatgga gtaaaattga atgaaaaatt atccgttact tttgacacct gtcatacgaa
    28321 tgatgccggt tataatatta aagaagactt ttcaggtgta atggaagagt ttgataagat
    28381 catcgggtta gatcggctta aagtactcca tatcaatgat tctaaaaatc cgcaaggttc
    28441 gcataaagac cggcacgcca atattggttt tggaacaatc ggattcgaca cgctgaataa
    28501 gatcgtccat gatccaatgt ttgacaatat ttcgaaaatc ttggaaactc cctatgtagg
    28561 caaagataaa aagaaagctt acgcaccata tggaaaagaa atcgccatgt tcaaagcgca
    28621 aacattcgat ccagaagtgt ttcctgagtt attagcagaa gaagtgatac tctaagattt
    28681 tctatctgac agcagcaaaa aaggaccagg cacattgcct tgtccttttt tgtttaagaa
    28741 acaaaggagt actttttgca gtctttttca tcatgcatct tctcccccct ctttttaaat
    28801 ttagacaaac aaggtatact ggtagaaaag taaaaaaacg aaaagtttca caagcaagtg
    28861 gcaaaacgtg attggaagga cgaaaagatg ctgaacactt taaaaccctt gatcaaccaa
    28921 ctaaaaaaga aacatacata ctttattctt ggcatagccc tattatttac aattattcgt
    28981 ttgtatttat atagtaaagc aatctatgga acagacatgt cggcaaccta tgatgaccag
    29041 ctgctgattc gctatgctca aaatattttg gacggtaaat ggctagggga gtatacgact
    29101 acaacacttt ccaaaggaat ctcctacagc ttatttttag tacttgcaca taaactgcat
    29161 ctttcttatg cagttttgtt cggcatgttc agtatttttg ccagtatatt ggttgcactc
    29221 gcgtttaaac cgattgtgaa gaacaagtat cttcttttaa gtatctattt ttattttttg
    29281 ttttctccga ttagttttac acatgaatat tccactcgaa catatcgtaa tgccctcgtg
    29341 attccggctg ttgagatcat tatcgcttgt ttattagcta tttattaccg caaatacggt
    29401 tctagcagaa aattgctgcc atggttttta gggctttctg tcgcttttcc tttcttttgg
    29461 ttcattcgcg aagattctat ctggcttctt ccttttactg tagtagcttt gggtatcagt
    29521 ttcgtccaga tcttgctagg gcaagacttt tctttcaaag aagtaaagaa gttaaaaaaa
    29581 cgactctttc ttctctcgaa acgggaatgg ctgaaacttt cattgttttt acttcctata
    29641 gttggctttt ccatcgtcta tcaatggatc gaatataaaa atgagacaac atacggtatt
    29701 gcaaccatca atgaccgttc cagtggagaa tttggacgtt tgatgaaaca actgatccga
    29761 atagatgatg aaacgaactt gaataaagaa aacagtgaga tatgggtctc acatgctgca
    29821 ctaaataaag cattagccgc atctcctaca ttccatactg tttctacccg tattgacgaa
    29881 ttatatacga ctcacgcgtg gactcaaggt ggaaaagtaa aagaattacc cggagatatc
    29941 atcttttggg cattgcgaga tgtggtaagc gaatctggtt attaccaaaa caatgcaaaa
    30001 gaaaccaatg atttttggaa aaaagtcaac caagaattag aagaagccta tcacaaggga
    30061 acaatcaaaa agaaaaaaga gttctatctg atgggatcag gagatggaaa gacgatgagt
    30121 gatctgccaa tcgtattcga tttccttcaa tcaggatacg cttatgcatg gcgatataaa
    30181 gatttccatc aaggaggaaa ttatagtttc ggaacgcaag aacaagtcaa acaggctcaa
    30241 gaattattaa accttccgct aatggagaat tggatcgata gtaatgaacc gagaacacct
    30301 aatttccaat taacaaaatc agcaaaagtc gcgaatgtca tcataaaaat ttaccaagtt
    30361 ctggctattc ccgtgctgat tgcttcaata gcagggatat tacttatgat actaggaacc
    30421 gtatttgcaa aagctgatcg tgcctattat agttcctctt tgattgtggt caccggatta
    30481 atactgacac aattggtttt tcttttcggt gtctcatggt tttgttctta tgcgccagaa
    30541 aaaaaggact attttttaag tgtctacact ggtgcaggcg tgccattaac gcaattcatc
    30601 gtagtaacaa gcatgctagt tttcagtaaa ttaaatctga aaaaagcttt atcttctaaa
    30661 taaaaagacc cttggtagct agatttttaa cctaactatc aagggtcttt gtgtggaaaa
    30721 atcagttttt attcgatttc ttctaccgtt tcctgcaaaa gctgttcata ttttttgttt
    30781 cctttatata aatcaccaat cgtccatgcc aacaggagaa ggacaagaac aatgattaca
    30841 taagcgatgt gatcagccaa tgctagttga gcggaagtgg cttgctcgcc gaagaaactt
    30901 tcgatctgtc ctggtaatcc tcgtaagttc aagtaagtta gactaatcac agagaaccag
    30961 ccaagaagtt ttacgatgaa tgaattttta aatcggctac ccatctccgc ttggctgttg
    31021 gtcatcatca gtaaaggaat catcgaaaac ggcaacgcaa aagccaagaa cacctgggat
    31081 tcattcatta agttattcaa agcggcatgt tcttcgatcg ttccttttgt acttgtaaac
    31141 aaaacacaaa tcaatacagg aatcacagaa agcaatcgtg tgaccagtct tcttagccaa
    31201 atcgggatac gcatatggat gaatccttcc atgatgactt gaccggtcaa cgtaccagta
    31261 atagtagagt tctggccaga agcaagaagt gctacagcaa ataaagtgga caagatacca
    31321 gattttgcta cagcaatcaa aataccattg ctcatagctg atggatcaga taaagcttca
    31381 tacagaccaa aaaaggaagg atcttggaca gaaccggttt tgaagacagc gacacccatg
    31441 atcaatagca gtgcattgac tagaaaagcc attgacaact ggatattaga atcccaagta
    31501 gaaaaacgta cagcttgtgc tacatcttct tcgttttggt gatcgatctt ccgagtttgt
    31561 gaaactgctg aatgaagata taagttatgc ggcatgactg ttgcaccaat aatccctaaa
    31621 gctccagtca gcggagtttc gccgccgatg accggtgagt ctgagaatgt atgactgtta
    31681 gggataaagc cagcaaataa actgtgccaa tctgggttag acaagccaac ttgatagaca
    31741 aaaacgaata aaatgacgaa aattaagcag acaaccagtg cttcgatctt acgaaacccg
    31801 acttttgtca gtagtaagag aacgaaaaca tctaatacag taataaagac ggcaatcacc
    31861 aatggaatat caaaaagtaa gtagagggcg attgctgcac caatgacttc tgcaatatct
    31921 gttgccataa tggctaattc agtcaagatc cataaaatga cacctaatgt cttacttgtg
    31981 cgtgctcgga ttgcctgtgc caaatccatt tggctcacga ttcctaattt tgctgccata
    32041 tactgtaaaa gcatagcaat cagactagac atcaaaataa ctgacatcaa taaatattgg
    32101 aaattttgtc ctccggtgat cgaagtagac cagttacccg ggtccatata cccaactgct
    32161 actaacgcac caggtccaga gtaagcaaga agtgttttaa aaaaactttt cccctttggt
    32221 acatcaactg ttccatttac ttcagctaaa gataaaccat ttgtatgttc aataagtcga
    32281 tgcttatgaa attgtttatt ggattgatta ttcaagaaaa aaccaccttt cctatttatc
    32341 tacgattaca ttctacacct caaaaagaaa aaattaaaga aaaagattcg gtacgcctaa
    32401 ttttttttca tggcgcgtct tgctagatga cgaaagaaat tttgttctcc tctaaaaaaa
    32461 ccactatttt ttgcatgaaa ctaaagaata cgcctttctt ttttcaataa actttagtaa
    32521 aataagtcat tgagagcaat tcggaaatag aaaggagaga tttatatgtt atcaacaggt
    32581 attgtcgtac ttattgctat tattgctttg ctaatcggcg cagtaggtgg ctttttccta
    32641 gcgcgtaaat atatgaaaga ctatttagaa aaaaatcctc ccgttaatga ggacatgttg
    32701 cgatcaatga tgatgtcaat ggggcaaaaa ccatctgaga agaaaattcg ccagatgatg
    32761 cagcaaatga agaaccaagg gaaaaaatag gttctgaccc aacgactttt tacgggctga
    32821 ttttttcagt ccgtttttta tttcgtaatt tgttagacta ttcagattat tcagcaaaaa
    32881 agacaagcac agacaatcct aggctcttga taaagacaaa ggaggagtaa aatgtcgatt
    32941 tttagaaaat taggctggtt ttttcgtcag gaaaagaaac attatttaat tggggttttc
    33001 tcgctattcg cagtggcctt ggtacagtta gttcctccaa aagtgattgg gatcatcatt
    33061 gatgagatcg ccgataaaaa tttatcaata aagatcattt tcgggtggat cttcctattg
    33121 attatcgctg cttttttaca atatcttttt cgttatatct ggcggacgaa tatttgggga
    33181 agtgcagctc gattggagaa agaacttcgg cagcagttat tcaaacattt tacagaaatg
    33241 gacaatctct tttaccaaaa atatcggact ggggatttga tggctcacgc aacgaatgat
    33301 ttgaatgcga tccagaatgt agcaggagct ggtatcttga cctttgcaga ttcagtgatt
    33361 acgggcggaa caacgatcat tgcgatgatt ttgtttattg attggcgctt aacactgatt
    33421 gcgctgattc ctctgccttt gctggccgtc acttcacgag tgctggggtc aaaactccat
    33481 gatgcttttc gtgattcgca agaagctttt tctagtatca atgataagac acaagaaagt
    33541 atcactggga tcaaagtcat taaaacattt ggacaagaaa aagaagatct agcggatttt
    33601 gaagaaaaga ttgaagatgc aattattaaa aacaaacgag tcaatttttt agactcctta
    33661 tttgatccat tcattacctt gattatcggt ctgtcttatg ttgcaacaat tattttaggt
    33721 ggaaagtatg tcttggaagg cacgatctcg atcgggcaga tggtctcatt tattagttat
    33781 atcggtatgc ttgtttggcc gatgtttgcg attggtcgat tgttcaacgt attggaaaga
    33841 ggaaatgctt cttatgatcg cgtcaatgaa cttttgcatg aaaagacgca tatcgtggaa
    33901 caactgggtg cagtgcagac cccggcaaaa ggcgagctga atgtagctat ttcctctttt
    33961 aattatccga aagaagaatc accagcatta gaacaaatca agtttgcgat ccaagaagga
    34021 gaaacattag gtattgttgg gaaaactggt gcgggaaaaa cgacgatcat caaattactg
    34081 atgcgtgaat acgatgatta tcaagggaaa atccaatttg gcggacgttc gatcaaagaa
    34141 tattcattga atgctttatt aggatcaatc ggctatgtgc ctcaggatca cttcttgttt
    34201 tcgatgacca ttcgagataa tatccgtttt tctaatccaa aactttcaga agaagaagta
    34261 gagcatgcgg cttctttagc atttatcaac aatgaaatca aggaattacc aaaaggttat
    34321 gataccttag ttggagaacg tggagtctct ttatctggcg gacagaaaca aaggatttcg
    34381 attgctcgag cattgatcaa aaatcctgaa ttattaatat tagatgatgc actctctgca
    34441 gtagatgcca aaacagaaga agctatcttg cataatttaa aagaaataag aaaagagaaa
    34501 acaactatca ttgcagctca ccgtttaagt agcgtgatgc atgcaaaaga aatcattgtt
    34561 gtcgatcaag ggaaaatcat tgaacgagga acccatcatg agctgcttgc actaggcggc
    34621 tggtatgaac aaatgtggga gaaacagcaa ttagaagcga agatagaagg aggcgaggcc
    34681 tgatggatga aaattatcaa tcggaatgga caaaatcgat tccacttaaa gaacagaaaa
    34741 agatcatcaa acgactgatg aagttcgcca aaccttttag aagaacgttc atcgttgcta
    34801 tcttatttgc gtttgctttg tctgtgatca atgtcctatt gccaaggatc atccaaactt
    34861 ttatggatga tcatctggct aaacagtcgg ctacgacaca agttattttg ttttttgctg
    34921 gtatgtactt atttggagtc atagtaaaaa gtatcatttg gtttttccaa tggtatttgt
    34981 attccatggc ctcattgaag acctatcagt atattagggt aaaattattt gaaaagctgc
    35041 atacactagg tatgcgttat tttgatcaga caccggcagg atcaaccgtt tctcgagtga
    35101 cgaatgacac agaaacgcta tttgaatttt ggtacgtctt tttgatggta ttgactggga
    35161 tctttgcagt cgtttcttca ttccttgcca tgtttcaaat caatggagaa attgccttgt
    35221 ggactttggt gtttttgccg attttaggaa tcgtcatctg gtattaccag aaattcagtt
    35281 ctcgtattta ccgaaatatg cgggaaaaat taagtcagct gaacacgaaa ttgaatgaat
    35341 cgatttccgg catgcgtatt atccagcagt ttcgccaaga agctcgttta gctaaagaat
    35401 ttgaacaagt gaacaatgag tatttagaca cgcgttatgc gatgatcaaa acgaattcga
    35461 ttctgttaag tccgatcatc aattttctat atgcattggc gattgcgttg gtgttgactt
    35521 tgtttgggat cgatgcgttg cattctcccg tagaagttgg actgatctat gcatttacga
    35581 cctatgtcca gggatttttt aatccaatgt ctcaaatgat ggactttttg agcatcttca
    35641 cagatgggat ggttgctggt agtcgtatct tgaagattat ggatacggaa gaatatgcac
    35701 cacaacaaaa ccctggagca gatcaaatga ttacttcagg taagattgag tttcgcaatg
    35761 taagcttttc ttatgatgga gagcacaatg tattaaacaa tatctctttt gttgctaatc
    35821 cgggagagac cgttgcatta gtgggacaca ccggaagcgg aaaaagctcg atcatcaacg
    35881 tattgatgcg gttttacgaa ttttatgaag gtcagatatt gattgatgat catgacattc
    35941 gagaatatcc aatggaagag ttacgtaaga aaatggggct ggtcttgcaa gatgctttct
    36001 tgttttatgg ggatatcgca ggaaatattc ggatgatgaa tccagctatc acagacgaac
    36061 agatacgcca tgcggctgag tttgttcaag caaacaaatt catcgatgaa ttaccaaaag
    36121 gatatcactc aaaagtgatc gaacatggtg ccagctattc tagtggacaa cgacagctga
    36181 tcacttttgc gcgaacaatc gtgactgatc ctaaaattct agtattggat gaagcaacag
    36241 caaatatcga tactgaaaca gaaggattga tccaagaagg attggcaaaa atgcgtcaag
    36301 gacggacaac gattgcgatt gcccatcggc tgtcaacgat tcgagatgca gaattgattt
    36361 tagttttaga caaaggtcat atagtagaac gcgggaatca tgaaacactt ctagcgcaaa
    36421 atggtttgta tgcagatatg tacaaactgc aaagcagtgg tatataaaag atgttgtgac
    36481 agtagaattt tgacctaagc actagagtga aaataaaaca tagcttctca tatttttcat
    36541 ctgtgagagt tagtgtcgta gtcaccagat ttaaaaaaag attcgacagc tagtcagaag
    36601 tgcctaatag ccactaagaa agcgttgaca aaagcatttg ctttcgataa tataaagaaa
    36661 acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt ctcccatggt
    36721 ttcatggacg gctttttggg tttttctttt tgttttggat acagccctat acatcatgga
    36781 aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat aaccagggat
    36841 gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa gaaagtgggt
    36901 gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga cgggctttca
    36961 caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat ttcttgtgaa
    37021 atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt gtatattgca
    37081 ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt tgtaccaaat
    37141 gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg gaaaaaaaca
    37201 gtgaagaaaa tctcggataa ataccacatc aagttatcag aagatgaagc cggatttatc
    37261 gcattgcatt tagtcaatgc acaaggcatt caaagccagc aagacaatac ctacctgttt
    37321 actataacaa tacaggatat tctaaatatc gtccgctatt actataagat tacatttgat
    37381 gaagattcag tccatttttt tcgattcgtc acacacttac gtttttttct gacacgcgtc
    37441 ctagatggag aaaaatcaga agtgggagaa gtttctgagg aattgtttca ggttattcaa
    37501 aaaaaatata aggatacgtt tggttgcgtt gagaaaatag cagacttttt agccgagacg
    37561 ttcaactacc agatgtccac ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta
    37621 gtgcatgaga atcgttgtaa ataaaaaaag gaggataaat taacaagaac gtatgaaata
    37681 agggtagtga cattcagttg gctaaacctt ccactaataa attggaggga atagaaatgg
    37741 gaaaatacca agaattagcg agaaaaatcg tagaaaatgt cggcgggaag gaaaatatca
    37801 atggtttgac acattgtatc acaaggcttc gtttcaaatt aaaaaacgaa gaaaaagcaa
    37861 atgatgagat cttgaaaaat atggacggta ttgtaacggt aatgcgagcg ggagggcagt
    37921 atcaagttgt cattgggaat catgtgccag tcgtgtttga agaggttata aaagcaggaa
    37981 accttacttt tgatgaggct gtttcaacag aaaaaatgag accgtttgat atgcttattg
    38041 atggaatatc gggctgtttc cagccattcc ttgctatcct agctgctggc gggatgattc
    38101 gtggactgac agcattcctt gtcttcttag gcgcgtttga ccgcggttct ggcacatttg
    38161 ttatgtttga taatatcggt gattctgtat tccaatttat gcctgttatt atcggtttaa
    38221 cagctgcccg taaatttaaa gtaaatgaat tcgtagggat gttgcttggc gcagcattaa
    38281 tgaatcctag cttgtcgctg gaagctttga gtggtgcagc agaagcacca ctaacgacca
    38341 tattttctgg cactattttt gaagcaccta tttatcaaac agtattcggc attccctgga
    38401 ttgcgcgtaa ctacgcttct tctgtcattc ccattatctt tattgtgttg ttagctagcc
    38461 aaattcagaa accaattaaa aagttagtac ctgagatgat cgcgaatttc ttcgttccat
    38521 tcttcaccgt tcttattaca atgcctttag gttttttact cgttggacca gtattcacat
    38581 tcgcaactga tattttaatg gctggatttg aaacactttt agcactttcg ccagttattt
    38641 atggagcaat cgtaggcttc ttctggcaaa ttctggtgat gtttggtctc cattgggcaa
    38701 tcgtaccaat gggattgatg caattttctg tgaatggttg gcaaaatatt atgacacctg
    38761 ttgcagtagt gtcttttgga caaactgcag cacttaccgc actctacttt aagcttagaa
    38821 atccaaaaga caaagcaatt gcaataccag ccattgtttc tggtattgtg gggattacag
    38881 agccagctat ttatgggttt acattaccac gtaaaaaaat atttattttt acctgtgtcg
    38941 gtggtgcgtt agggggcgcg tatagtgggt taatgaattt aacttcttgg aatcaaggtg
    39001 gactaggcat ctttacaatt cccaactata ttagaccaga tggtgattta acagatgtga
    39061 ttaatgtatt gattgggatc gccattgcga tggtggtatc ctttacgctg acttttttct
    39121 tttggaagga tgaagcaggt gaaacagatg atatacagaa aaaatcaggt aaagaaattg
    39181 taaaaacacc gatacaagga caaatcgctc cactaaatgc agcaaaggat gcagcttttg
    39241 cacaaggaac actaggtcga ggaatcttga tctaccctga aaaaggagaa gtacgtgccc
    39301 cttttgacgg aacagtcatg accctgttcc cgacgaaaca ggcaattgga atggtttctg
    39361 aaacagggct ggaattgctg attcatgtcg gattggatac tgtgcagttg gaagggaaat
    39421 attttgaaag cttagtgcag caaggataaa gagtgaaaca aggcgatctg ttgctacgct
    39481 tcgacaaaaa gaaaatcgaa aaggaaggat acagtttaga aacacctgtg atcgtcacca
    39541 attacacgga ttatttggat attttggaaa accaagggga gacggtcggg ccagacgatg
    39601 cacttatcac cgcactgacc tgaacggtta ggtgaagaat ggactaggct taactattct
    39661 ttttattcca cttctttatt ttcaaaattg aaaggctctt tcatgatggc tgagctgata
    39721 gggcgtttat cgaatttttg accgacagaa attagatgga ctaaattctg ttgataattt
    39781 tctgcaaaaa cacaactata taacacgtct agaagataac aaattgatgt attgatcgta
    39841 aaattaccaa tttttgaata cagtcgctcg cgtgtcgtaa gtcgcaaagc ggctgtactc
    39901 attgcagata gcgttgaatc accgatactg gtcaaagcta aaattggaac atgattttgt
    39961 ttcaatattt ctgcggtacg aaggatcaca ttgttttcac ccgtataaga aatcagaatc
    40021 ccgcacatat cactcgtgca attgtacgct tcatagaatg attcaccaaa agtggtgaca
    40081 atcgtgacat ccattttgat gcgcttcatt ttcagcatga aatcttgaga aatcaaagta
    40141 ttcgagttac tggcgaaaat cacgattttt tttgcttgaa gcattagttg tttggctttt
    40201 tgcagttggt catgatgaat gagagaaagt gtatcatgaa tcgtcatctg ttctaaagaa
    40261 gccattttat ttgcgatcgt cataataccg tctgtggtgg aaaaaggaag attcgcatct
    40321 acgtcttgaa aattcccttc aagataggct gtttcagcta aaaaagcatt acgaaaactg
    40381 acccagccat caaaacccat tttttttgct acacggatga gtgtagaggg gtggacataa
    40441 catcgctcag caatcgcttg gatggtcatt tcgccaagtg ctggagaatc gcttaagaga
    40501 aactggatca cttcattttc tgaaggagaa aaggagattt ctttgatttt ttcacgtaat
    40561 aacatgtaga taccttcttc ttattttttt acgtaaatta taactttttt gaaaatgatt
    40621 tgtaaacgga tttatactat ataacttaaa atagacattt tttgacaaga tatttacaaa
    40681 aatccactag aaaacagtaa attttgtaaa tgttactgga aagaaacaga tttctattgt
    40741 ttgttttgtt tacaaaaaag ataatgaaag tgtaaaaaag taaaggagtg agaacatggc
    40801 aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt tacacccccg agttgatgga
    40861 aggatttatc aaaagatatg aggaattacc gatacgagaa atctggctcg tagatgtaga
    40921 agaaggaaaa gaaaagcttg agatcgttag tcagatgtct cagcgtatgt gggatgcttc
    40981 tccgtatgaa gtcaagatct atgcaactct ggatcgggaa gaagcattga aagatgcgga
    41041 ttttgttacg actcagtttc gtgtcggtca attagatgca cgaatcaaag acgagcggat
    41101 ccctagttat tatgggatga ttgggcaaga aacaaatggt gctggcggga tattcaaagc
    41161 ttttcgtacc gttccagtcg ttttggcaat cgttgaggac atgaagcgtt tatgtccaaa
    41221 tgcttggctg atcaatttcg cgaacccaag tgggatggtg acagaagcag ttatcagata
    41281 tggcaaatgg gataaagtga ttggattatg caatgtgcca gtgatggcga tgatgacaga
    41341 accagcaatg cttggcaaga aaaaagaaga gctgatctac aagtttgccg gtctaaatca
    41401 tttccattgg cacaaagtag cagataataa aggaaatgac cgcacggcag aattgattga
    41461 tcgtttatac gatgaagaga atggattacc gaaaaatatc tttgatatcc cgtttttcaa
    41521 agaacaatta aaacaaatga acatgatccc ttgtggctac caccgctatt actatcgtga
    41581 agaagaaatg ctggaacatg ctttagaaga atatcaaacg attggtacaa gagcgcagca
    41641 agtgaaagaa acagaagcag agctatttga attatacaaa gatccagaat tagaccataa
    41701 acctgaacaa ttgcaaaaac gaggtggcgc ttattattca gacgctgcct gtgaaacgat
    41761 tgcttcaatc tatggaaata aagaaacaca gatcgtggta tctacaaaga atgatggagc
    41821 tgttcctgac ctgccggcag attgtgtagt agaagtcact gcctatgttg gcgcacaagg
    41881 agcgagaaat gtagcgtttg gttctcttcc tgctgctgaa agaggctggt tgcaagtaat
    41941 gaagaatatg gaattgctga cgatcgaagc agctgtgact ggtgattatg ggacagcttt
    42001 acaggcgttt acgatcaatc cacttatacg atcaggaaag acagcaaaac ggatcatgga
    42061 tgagttgttt attgcacacc agcagtattt accgcaattc aaggagacaa tcagccgatt
    42121 aaaagaagaa ggaatcgaag ttcaagatga actggcaaga gaattatcag gtaatacaca
    42181 tataatggag gaagtataat gggattcaga aaagattttt tatggggcgg tgctacagca
    42241 gccaatcaat gtgagggcgg ttatgatgaa ggtggacgcg gactagcaaa cgtagatgtt
    42301 gtgccaatcg gaaaagaccg ttttcctgtt atcaccggaa agaaaaaaat gtttgaattt
    42361 gatgaggatc atttttatcc agcaaaaatt gcaatcgata tgtatcatcg ttataaagaa
    42421 gatattgctt tgtttgcaga aatggggttc aagacttatc gaatgtccat cgcctggagt
    42481 cgtatttttc caatggggga tgagaaagag ccgaatgaag ctggcttgaa attttatgag
    42541 gatgccttta aagaatgcca aaagcacgga attgagccac ttgtgacgat tacgcatttt
    42601 gattgtccaa tgcatttgat caaagaatat ggtgcgtggc gttccagaga actcgttggt
    42661 ttttatgaaa atctgtgtcg agtcatcttc aatcgctata aaggacttgt caaatattgg
    42721 ctgacattca atgagatcaa tatgatcctt catgcaccat tcatgggcgc agggctgtat
    42781 tttgaaaaag gagaaaatga agaacaaatc aaatatcaag cggcacatca tgagttggtt
    42841 gcaagtgcca tcgcaacgaa gattgctcat gaagtagatc cggagaacaa agtaggctgt
    42901 atgctggcag caggagcata ttatccatac acttgcaatc ctgaagatgt ttgggcttct
    42961 agaaaatcag atcgagaaaa ttatttcttc attgatgtcc agtcacgagg agaatatccc
    43021 gcttatgcat tgaaagaaat ggagcgaaat ggcattcaga tcgaaatgga accaggggat
    43081 gccgcattgc tgaaagaaca tactgttgat tttatctctt tttcttatta tgcttcccga
    43141 gtttcgacta ccgacaacga actcttggaa cagactgcag gaaatatctt tgcttctgtc
    43201 aaaaatccat atctgcaagc aagtgaatgg gggtggcaaa ttgatccatt aggcttgaga
    43261 ataacgatga atgatattta tgatcgttat caaaaaccat tgtttattgt agaaaacggc
    43321 ttaggtgcag tcgatacgcc agatgaaaat ggttatgtgg aagatgatta ccgaatcgat
    43381 tacctggcag cgcatatcca ggcgatgaag gatgcagtag aactagacgg agtcgatcta
    43441 ttaggctata cgacttgggg atgtattgat ttagtatcag ctggtaccgg tgagatgaag
    43501 aaaaggtacg gattcattta tgtcgatcgt gataaccaag gaaatggcac attagcccgt
    43561 tcaaagaaaa aatcattcaa ctggtataaa aaagtaattg attcaaatgg agaaaatctt
    43621 tcgaacgatt gatcaatgat ttataaaagt ctattgtttt tcagcggatt tttcgagtat
    43681 aataaagcta ttcgagaaac gcaagcagaa caatagactt tttttcgcat tggctgtaaa
    43741 gtatgcttac ttgattaagt aaaggagaat ctacatatgt atgatgtaat cgttattggc
    43801 gcaggtcctg ctgggatgac tgctgcttta tatgcttcac gttcaaactt atccgtattg
    43861 atgatcgaac aaggtgcccc tggcggccaa atgaacaata cggcagaaat cgaaaattat
    43921 cctggattcg attcgatcat gggccccgat ctagcttata agatgtatga aagtgtctca
    43981 agatttggga cagaaaatgc ttatggaatc gttcaagata tcaaggatca cggcagccat
    44041 aaagaagtca tctgtgaaga taaaagttac gaaggaaaaa cagtcattat cgcaacagga
    44101 tgtatccacc gtaaattagg cgtacctggc gaggaagaat ttgctggacg aggcgtttct
    44161 tactgtgcag tctgtgatgg tgcgtttttc cgtaacaaac gtctagtagt catcgggggt
    44221 ggggactcag cagtagaaga agccatctat ttgacacaat ttgcttctga agtagtgatc
    44281 gtccaccgta gagatgaatt acgtgcacaa aagatcatcc aagaccgtgc atttgcaaat
    44341 gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa tcgttggaaa cgaaatggta
    44401 gtgacaggtg tgaaagcacg taatgtcaaa accggcaatg aatttgattt agcagcagac
    44461 ggagtattca tctatgttgg actggatcca ttaactgcac cttttagaga agcaggtttg
    44521 acgaacgaag ctggctggat tccaacagac aacgaaatga aaacgaatat gccaggcgtt
    44581 tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa tcactacagc tgttggtgac
    44641 ggcggtattg ctggacaaca agtgtataaa tacttggagt ctttagaaga aacaacagca
    44701 taagtttaaa aagagtagaa gaacgggttc aattgttctt ttactctttt ttttcgcgtg
    44761 aaatttactg aatctgattt atgcgaacac gtccaacacc ttttgataaa agaatccttt
    44821 cttgatttaa gcgttttttg gtcctcatac tttcatatct aataagtgaa tgctatactt
    44881 attaagaacc atatttcaga aagaggtgtt attttcatgt cttgggaaca agtgtatgaa
    44941 caatgggtac atgaagaaaa tttagaagaa aacttaaaaa aacaattaaa tgatctgggt
    45001 gaagatcctg aaaaacttga agatgctttt tatgcaccgt tggaattcgg tactgcaggt
    45061 atgcggggaa tcttaggacc tgggatcaat cgaatgaaca tctatacggt aagacaagca
    45121 acagaaggac tagcacgttt catggataca caaggtgaag aaacaaaacg ccgaggtgtc
    45181 gctatcgctt atgactcacg ccatcagtca ccagaatttg caatggaagc agccaaaaca
    45241 ctagcacatc acaatatccc gtcttatgta ttcgaaagtc tgcgtccaac accagagctt
    45301 tcttttgcag ttcgtcattt gaagacgttt gcgggaatca tggtaaccgc atctcacaac
    45361 ccagcggctt acaatggata taaagtctac ggagaagatg gcggacaaat gcctcctgca
    45421 gacgcagatg cattgacgaa atatgtccgt gaagtcagca atccgctaaa agtcgaagta
    45481 ttatctgacg aagaagctaa acatagcggg ttgatcacga tcatcggaga agaagtggac
    45541 gctgcttatc tagaagaaat caaagcagtg acgattgatc gtgaattggt tgaaacaatg
    45601 gggaaagatt tgaaacttgt gtacacgcca cttcatggaa cagggaaaat gttgggcgaa
    45661 cgtgcgttga aacaagctgg attcgaacaa ttcgtactag ttcccgaaca agctgtggca
    45721 gatccagatt tcactacggt caaatcgcca aatccagaag aacattcagc ttttgaatat
    45781 gctatacgac ttggagaaaa agaaggagct gacttgctga ttgcaacaga tcctgatgca
    45841 gaccgtttag gcgcagcggt tcgtttgcca gatggttctt atgaagtatt gactgggaac
    45901 caaatcggag cattgtttgc tcaatatatt ttggaagcac atcaatcaag aggcacttta
    45961 ccagaaaacg cagcggtatt gaaatcaatc gtttccagcg aattgcctac agcaattgcg
    46021 gaacactacg gtgctaccat gttcaatgtg ttgacagggt ttaaattcat tgctgaaaaa
    46081 atccaacaat ttgaagaaga ccattcacat acttttatgt ttggattcga agaaagttat
    46141 ggttatttag ttaaaccttt tgttcgcgat aaagacgcga tccaagcatt gcttttatta
    46201 gcagaagtag cagcttacta taaaaaacaa ggcaaaacgt tgtatgacgg cttgcaaacg
    46261 atttttgaaa cctatggcta tttcgcagaa aaaacgattt ctgtgacaat gagcggacaa
    46321 gaaggttctg cgaaaatctc tgctttgatg gaaaaattcc gtaaagaagc accgacagaa
    46381 tttgccggag caaaagttat tcaaacagaa gactttaaag aattgacgag aacagaccgt
    46441 gaaggaaacg tagaaaaact aacaacaccg ccatccgacg tattgaagta tacattggaa
    46501 gaaaatggct ggatcgctat ccgcccttca ggaactgagc cgaaaatcaa gttttatatt
    46561 ggtgtaaaag gaagctctac agaagacgca gccgaaaaag tagcctctta tgaagcgatt
    46621 atcaaaaaga tgacagaaga ataaaaaact tgagactgat ggaaaaagtg gtaaacttta
    46681 gaagtcagtc ttttttcaag ttggaggtga atggattgcc agacacgcaa aaatcaaaag
    46741 tagagaaagt cagtcaatta ttcaaaatgt tgagtgatcc cactcgtctt aaaattctgc
    46801 tatatctgaa agacggggaa caaaatgtga cagcaatcac ccaagcagtg gaaatggagc
    46861 agtctgctgt atcgcatcaa ctacgtttat taagagaaaa tcatgtcgtc aagtctcatc
    46921 gcgagggaaa agcgattttg tatagtcttg atgattctca tgtactggat attttgaacc
    46981 agacactcaa acatgtggaa catcaataac gataggtgaa aatagataaa cactggagtg
    47041 tgacaacgaa gaaatttcgt tgtcacactc cagtgttttt taagaaaaag acaaaaatga
    47101 ttaatcgctt ttttctgttg tttcagttcc ataataacta tgatcttcta ggtctaaagc
    47161 attcaaaata agcgaagccg tttcttcaat agataaagat gccacattga tcactacaca
    47221 tcctagtttt tcataaagat cattcgcaaa atcgagttca gctctaattt tttcgatatc
    47281 ggaataagca gtatcagggt ttaatccgta agagcgcatc cgttctttac ggatattatt
    47341 taaaatatct gggttattcg ttagtcccac gattttttta ggatcgattt cccacagctg
    47401 tttgggaata tgtgcttggg gaatcaaagg taaattagca acttttaaat ttttatttgc
    47461 taaaaacaag ctcaaaggcg ttttggaagt tcgggatacg cctagcaaaa cgacatctgc
    47521 ttctaaaaaa ccacgaggat ctttcccatc atcgtatttt acagcaaatt ccattgcttt
    47581 gatccgtttg aaatagttct cattcaataa atggagggca ccaggttgcc gaattggctt
    47641 gataccagtt aaacgttcaa tttctgatat cggtggcgtc atcacatcga aactaaataa
    47701 ttgatgttct tcacaaaaag aacgtgttat ctcgatcagt tcttgattga ttaatgtatg
    47761 caggacaagt gctttttctt ctaatgcatc ttgcaatgcc tgccttaatg ttttttcatc
    47821 tgtcacgaac gttctgcgga ataggttgaa ctcaacggta ggatactgag ccatagtagc
    47881 ttgcgctagt tttgtcgcag tttctccagc agagtcggag atgacaaaaa aagtaacgat
    47941 ttctgtagaa tcggaagaca tggataaaac ctcacttgca ttttttttat gtttattata
    48001 ccatactcta gaatgcgtac ttcgtaatca ttttgatttg gaggaataag tcatgaaaaa
    48061 acagacattg atcgaacaat cattagaaac attgaaaaat aacggtttta aatatactaa
    48121 aaaaagagaa gcattgttaa cttatttagt gaagcgtaac cgctacgtat ctgctaaaga
    48181 agtttttgac tatatgaatc aggaattcaa aggggtaagt tatgatacga tttaccggaa
    48241 tctccatgat tttgcagaga tcgatttact ggaagaaacc gatttaaatg gtgaaatgaa
    48301 gtttcgtttt cattgctgtc agggggaaat cggccatcac catcatttta tctgtacagt
    48361 gtgcggaaca acgaaagaaa tccatatgtg tccaatgaat tatttcgaag aacagctagg
    48421 tggatgcacg atcgaagggc atagattcga gattttcgga agatgtgaaa attgtcaata
    48481 aggatttaaa taaaataaca tttgataatt taaaaaaaaa gcaagatttc cttgacggtc
    48541 aaaacagcat tagatataat atttacagga cagtcttgtt ttttgagata tatttcaata
    48601 acaattacgg ttacttttta agctcggagg gagggaatta acatgtcaaa aacagtcgtt
    48661 cgcaaaaacg aatcacttga cgatgctctt cgtcgcttca aacgttccgt gtcaaaagcg
    48721 ggtactttac aagaatctcg taaacgtgaa ttctacgaaa aaccaagtgt aagacgcaag
    48781 aaaaaatctg aagcagctag aaaacgcaaa aaattctagt tctgtgatga attggagttg
    48841 gtagtgtgtc actactaagt agattaaacg atgacatgaa agcagcgatg aaagcgaaag
    48901 ataaggaatc tttacaagtc atccgaatga tcaagtcatc tatacaaaac gagcagatta
    48961 aagaaggtca cgacttgaca gaagaagaag aactaacggt gttgtctcgc gaaatgaaac
    49021 agcgacgtga ttctttgcat gagtttgaag aagcaggacg tgacgattta gctgaaaaag
    49081 taaaaagtga aattgtaata gtcgaaaaat atatgccgga acaactttcc gacgaagaaa
    49141 ttcgacaact tgttcaagaa gcaatcactc aaactggtgc ttcatcaatg aaagaatttg
    49201 gcaaagttat gggtgcaatt atgcctaaag tcaaaggaaa agcagatgga aaccaagtga
    49261 atgcgattgt gaaagaacta ttacaatctt aattttatca tttaaactgc ggacagtgat
    49321 tgtccgcagt tttttgtgtt cgtaaaaaaa gaactaaaaa acagctagaa gaaagaataa
    49381 gattctttct tctagctgtt tttttcctgt ttaatatctc ttatgttaaa ttgtgtttga
    49441 tttggttatt cttctggtag ttcatttgct tcttgtttat accctgatag gcgataggcg
    49501 taataaaaga gtaagctgcc agcgtaacca agtaacgttg ggaccatcca gccaagtcct
    49561 aatgcagcaa aaggaacgtg atgatgataa aatcctagta agcttgctac gacagtttgc
    49621 tgactcacta atgcaggcga atttgccaac atatccaata cggctggaac ggcagaaaag
    49681 aacatcgtta cttgatagac aaatggactc ttttggaaga attttgctgt taaggaaagc
    49741 aagattaacg caagtgacaa tggatacaaa agcatcaata ctggtaaaga ccattggatg
    49801 atattatcca atccggcatt cgcaacgaca aaagagacaa aagtcgttaa ccgcagccat
    49861 gtcatataac tgactttagg gaacagttta tggaaatctt gtgcaaaaga aacaacgagt
    49921 cccatagcgg tagtgaagac acctaatgtg accattaccc ctaagaagct agcaccgaag
    49981 ttacctaaat aaaaattagt gatctgagaa agtgcagttc cgccattttc tgataaaggc
    50041 atcttattca aactcatcgc accgagaagg accagaccaa aataaatcat gacttctaac
    50101 gcaacgctca ggctaccagc ttttgctgtt aattctgtga cttttttatc tttaaaacca
    50161 agatcacgaa ctgcatggac aaaagtgaca cttaaagcaa gtaaagctac tgcatcaacc
    50221 gtgttgtatc cttctaaaac gccatttgtc aaagcgttcg tctgataaga atctgcagca
    50281 gattggttta gattccccat gggttggaat aaagaaacga caaaaatgat agctaataat
    50341 gcaagaaaca aggagtttaa atactttccg acatatttga ccaaacgtgt tggatgtaca
    50401 gtaaggaaat aagcaagtaa aaagaaaaga gcagagaata aaaacatacc taatgaatca
    50461 tatttttcag gcaaaaaggg tttagcagcc atttcaaaag cagttgctgc tgtccgcggt
    50521 gtaccaaaga atggtccgat cgttaaatga acaaggatca agaacaaggc agcataccat
    50581 tttccgacag gtttagctaa gtcatacaga ccatcacttt ttgtcactac tacagctaag
    50641 atagctaata atgggaaaag agcgcctgag atggcaaatc ctacaccagc gctgatccag
    50701 tttgatccag ccatttgacc taagtggaca ggaaagatca gattacctga cccgaagaac
    50761 atcccgaaga taagtgaact gaccatcaat aattgcttcc atgaataagt ttttgtttct
    50821 tccataaatt tttcctccaa taattatcat tttgagtaac aaaataaacg ttgcagaaac
    50881 tagaaatgcc actaatatca gattccatga aaaccaccac cttaaaatat aaaaaaaagc
    50941 ccttttccac ttgggaaaag ggctctagat tctagaacgg taccaccttt tttcgcttaa
    51001 aaaagcttct tcattcacgg acgcttttcg ataaagttat ccgctagcct gataatggag
    51061 gcagccacaa tgatctacgt tttcgacatt gactcggaaa tgatgttcgt ctattcgatt
    51121 tattgtcatt tctcacttac atgactcact gggaataaat cgctgaatag cctactgttt
    51181 tcctcgacgc tttgctgtat atttatttgt tgacgttata atacacctgt tttttttgaa
    51241 atgcaagaat atttcaaata ttttgttttc tttttttgaa acaagaaaaa tacccagaga
    51301 atacttgcta agttagacgg aatacttgtc ctctatctta tgcatagctt acatgatcag
    51361 ctttttttat tcaaacgaga cctttagaga atggaagcag ctatttttta acgtttcttg
    51421 gtacttatgg ttaagcaact cttttagaac cttttcttat ttgctatata gtttcttctg
    51481 agtatggtaa aataggaaag aatgaatcga aaaaaagggg atgtctattt ttgacggaag
    51541 agtctagttc tttagagatt agattgacgg ctgctgatga tgtcagcatg cttttaggtg
    51601 cacacgacaa gcatataaaa ctgattgaag aaacaacaga aacaaccatc catacacgtg
    51661 gagaaatcat tcagatcatt ggcgaacaat cccagatttc cttggctgcc tcagtcattc
    51721 ggacattgca ggaattgatc aaacgaggaa tccatgtaag tacaccagat gtcgtgacgg
    51781 cactgaaaat ggggcaaaaa ggcacattag attattttgt tgaaatgtat gaggaagaga
    51841 tcgtcaaaga ccgtaatgga aaaccaatac gggtaaaaaa cagcggccaa aagaaatatg
    51901 tcgaatcggt agccaaacat gatatcgtat ttggcgtagg accagccggg acaggaaaaa
    51961 cttttcttgc agtagtatta gcaatcgctg ccttgaaaaa aggtgaagtg caaaaaatca
    52021 ttttgacgcg gcctgctgtg gaagcagggg aaaatctagg ctttcttccc ggggatctaa
    52081 aagaaaaagt agatccttat ctgcgtcctg tttatgatgc tttataccaa atttttggat
    52141 tagatcatac gaaccgattg atggaacgag gcgtgatcga aattgctcct ttagcttaca
    52201 tgcgggggcg gacattggat gatgcattcg ttattttgga tgaagcacag aacacgacag
    52261 ttgctcagat gaaaatgttc ctgacacgtc ttggttatca atcaaaaatg attgtcaatg
    52321 gagatacgag tcagattgat ctgcctcgag ggacaacaag tggtcttgtt catgcagaac
    52381 gaacattaaa gcaaatcaag aaaatcgatt ttgttaattt tgaagcaagt gacgttgttc
    52441 ggcaccctgt agttgccgaa atcatcaaag cttatgaaaa ggcagatctc catcaagaat
    52501 aagctttgct taggaggata agcacatgtt gaataaaaca ctgcagaaac tacaagaaaa
    52561 acttggaaag gcagcattgc ctttcctgct gttcttgttt tttatcatta tctgcggcgt
    52621 tacatttagt agtgtcaaac aaaaggccaa tgatttcaga gaaggccaag tagcagaaga
    52681 aagtatccgt gcgaacaaaa cgatcgagaa cacggaagaa accgaacaaa aaagaaaact
    52741 ggcagcagaa gcagtcactc cagaatacac ttaccaaaaa gatcttgcag atgaccagaa
    52801 caatcggatc aagcaactat ttgaattgat cgataaaacg aatgatgcca tcaacaaatc
    52861 ttatgacgaa aagattgata aggcaaagga agatgaaaat atcccaaagc ctactacaga
    52921 agagcggatc gccgcgttga agaaaaattt tgaaaatgtg aatgcagaaa atgttgcgtt
    52981 ttatcaaaaa ctaccgaaca atttttattc gacgatattt gaaatgactg aaactgagat
    53041 ccatactgtg agggatgaga gtctgaagct cattgacgaa cagatgagca aacaagttcg
    53101 tgaaagtgaa ttggaagctt ttaaacaaga agcagaagag caaatccaat atttgaatgt
    53161 cacaccagca caacaacaaa tgattcgcta cttagtggat caaggtatcg tagtgaatga
    53221 cgtattgaat gagaaaaaga cagaagaatt gaaacaatcc gcacgagaag ctgtacaacc
    53281 agtgatgatc ttccaaggag aaatcattgt tcgtgaaggt aatcaaatcg atgcttcagc
    53341 tatgaaaaaa ttggaattac taggactgac taatcaaaca acttcgattt ttccgctaat
    53401 tgccatggtt ttagctgttt tgcttcaaat cggcgtactg atttataatt cacttcaatt
    53461 ccaaaatgca ggagaaagaa caaaatacgt gcttttctat gtgacagcta tgtcagtcag
    53521 catagtgctg atgaaatttt tccaattatt ccaaacggaa caagctactt atatcccgct
    53581 gttttatccc gctgcttttg caccacttgt tttaagcttt ttcttgaacc gaagagcagg
    53641 gatcatggct ggtattttcc aagcagtatc tgcacttttc attttttatg attcgattgg
    53701 cacgaacttt ctaacgatta tcttgatgtc ttatgttttc tctgggctca tggcaacgat
    53761 tgtcagacgc aaacggatct cagaacaagg attatctgct gccatgtggg tcattatttt
    53821 tcctgtgatg atggatgtca ttttgatcgt ctatcaagga atgagctttg gtgatgcaac
    53881 gacttggtta atgcttgtct gcgggttcac tggtgcactc ttctcattct tgctgacgat
    53941 cggtctacac ccatatattg aattgatggt caatgatgac agtgtaattg tattaaatga
    54001 actcagcaat ccaaatcatc ctttattgaa acagttgctg gaagaagcgc caggtactta
    54061 tcatcatagt atgatggtag ctaatctaag tgctaacgct gtagcagaaa tcggcggacg
    54121 ttctttgcta actcgagtag cttgctatta tcatgatatc ggcaagatca aacatgctag
    54181 ctttttcgtt gagaacttgc cttcaggagc ggaaaacccg cacaatttct tattgccaga
    54241 agacagtaaa catattatct ttggacatgt gacagatgga gccaaaatat tggaagatta
    54301 taatatgccg caaatggtga tcgatatttg caaacagcat catggaacca cgctaatgcg
    54361 ttatttctac atcaaagcga aagaacgcaa tccggaagta acagaagagc aattccgtta
    54421 tcctggacca aaaccgcaaa gtcgtgaagc agggatcgtc aatattgctg acagttgtga
    54481 ggctgcagta cgggcaatgg atcatccgac gagcgaaaaa atcgaaagtt ttgtccataa
    54541 tcttatcgaa gagcgtattt ccgatggtca attagacgat agtggactga ccttgaaaga
    54601 aatccgaaaa gtggaaaaat cattgatcag cggtttaagt agcaccttcc attctcggat
    54661 caaatatcct aaaatgaaaa cagaagttga gaagataaag gaagaacaag aagaatagaa
    54721 agaagcgatg tataatggat attaccttta tggatgaaac aaaccaagtc tcagaagaga
    54781 aaatgaaaga aatcgatgac ttgctgcaat ttgcagcatc ttatttaaag ctgccagagg
    54841 aaacagaaat gtctgttact ttcatggaca atgcggcaat ccaagtaatc aatcgtgatt
    54901 accgtggaaa agatgcaccg acagacgtca tcagttttgc tctggaagaa gaaggcgaag
    54961 acgaaatccc agtgattttt gaagatggag agaatccatt gcctcgtgta ctaggagaca
    55021 tcatgatttc tattgaacgt gctgctgaac aagcagaagc ttacggccat tcatatgatc
    55081 gggaacttgg ctttttagcg gtacatggat tcttgcatat caacgggtat gaccatatga
    55141 caccagaaga tgagaaagaa atgtttggtt tacagaaaga gatcttggat gcctatggac
    55201 tcaaaagata aaaacgtaga gaaaaacaaa catttcctga tgtctgtgga atttgctgtc
    55261 caaggagtca aaaccgtttt tgaggaagaa cgcaatatga ggaaacatgt tgcgttcggt
    55321 atccttgctt tatttatggg cgtgattttc cagcttgatc ggatagagtg gttatggctg
    55381 cttttagctg tttttctcgt ttggatagta gagatcatca atactgtttt tgaaaatgta
    55441 gtggacatgt ttacggattt tcattttcat ccgattggga aaaaaatcaa agatatggca
    55501 gctggagcag ttttagtgac cgcttgtttt gctgtcatca ttgggttgat tttatttgtc
    55561 cctaaaatat atcatttgtt gtttaattaa aaaggagaac gtatatgcca gaacataaat
    55621 cagggtttgt cgcaatcgtc ggccgcccga atgtaggaaa atccacatta ttgaaccgaa
    55681 tcgtgggtca aaaaattgcc attatgagtg acaaggcaca gacaactaga aataaaatcc
    55741 aaggagtata tactacacca gaagctcagc tgatttttat cgatacacca ggaatccata
    55801 aaccaaagca tcgtctagga gattttatgg tagaagcagc ttacagtgcg ttgaaagaag
    55861 tggatgcagt cttatttatg atcagtgctg atcaaaaacg cggccgggga gacgatttta
    55921 ttattgaacg tctaaaaaac gtgcattctc cagtttatct gattatcaat aaaatcgata
    55981 agatccatcc agacgagttg ctgggaatca tcgaagatta ttcgacgcag atgccttttg
    56041 cacaagtgat tccgatctct gctacagaag gaaacaatgt cgaacgctta atggatgtat
    56101 tagtcagtga aatgccagag ggcccacaat attttccaga tgatcaagtg actgatcatc
    56161 ctgaatactt tatcgtttct gaattgatcc gtgagaaagt cttgttttta acacgagacg
    56221 aagtgcctca ttctgtcgca gtcgttgttg attcgatgaa gcgaaacgag aatgataaaa
    56281 tccatatcca ggccacgatc atcgtcgaaa gagatagcca aaaaggaatc attatcggca
    56341 aaggcggaaa aatgctgaag caagtaggaa cgaaagcaag aaaggatatc gaaaatcttt
    56401 taggagacaa agtatttcta gaactttggg tcaaagtcca aaaagactgg cgagacaaaa
    56461 aagtctatct gcaagacttt ggctaccgaa aagacgaata ctgatattct gaacttctta
    56521 taggttaagt atagaaataa actggataaa aatccgcacg ttgagaaaaa cggcggaaga
    56581 tcgaaacata aataatctgg atcggcagaa tctgcttttc cagatttatt ttttaggagg
    56641 gagaatatga gccagcttgc agaatcgaaa ggcttgattt tatctagccg taattacaaa
    56701 gaaaaagata aattagtcaa aatctttacg gaatcttctg gcaaaatgat gttttatatc
    56761 aaaggaatcc atcggcaaaa tcagccatta gctccggctt tgctcccctt taccgaagca
    56821 gtctatatcg gtaattttcg aagtgaaggt ttgtcgtttt taagcagtgt caaagacgtc
    56881 catgcgtttc ggaacatcca agaagatatc tttttgagcg gttatggtac ctatttgcta
    56941 aatctagtag atgcagcaat cgaagatcat caatatgacc cgcatttatt ccaatttacg
    57001 caacaagctc tgcagcggat ggatcaagga gatgatgcag aaatcatcac gaatatcttt
    57061 gaagttcaaa tactgcaacg cttcggaatc gcgccaattt ggacgcactg tgtcgtatgc
    57121 ggggaaacaa aaggaaaatt tgactattct tccaaatctg gcggtgtgat atgcgagaaa
    57181 cactggccaa tggacgaaca ccgttatcat gcagatcctc gcgctgttta tttcgttcga
    57241 atgttttcgg ctatttctta tgataagatc agtggaatcc acttgaaaga agaaacgaag
    57301 caggcaatcc gtgacctcat tgaccagttg tatgatgaat atgtcggtat tcatctaaaa
    57361 agcaaaaagt tcattgatca aatgaagtct tgggcacacg tattaaagac gccagagaaa
    57421 aatatgcctg aaaaagagat acctgaaaaa gaagaataaa tgaaagatac aacaaaaaaa
    57481 tcaaaactat ttgacaaata cagggacttc cgtctattat taaccgtaga ttaaatagat
    57541 cgttcactga gaggatctat tatataaaca agaaaactgc aaaatgaata cgtacattga
    57601 ccaagatcag taaaaaacac gtggtttagc gagttcggga gagtggaagc cggacccaaa
    57661 cgatttttga agaggcactt gcgagtacga taaatgaagc tgccgaatct ttattcggaa
    57721 gtagggtgga accgcgaaat tattcgtccc tatgtctgta atcagacata gggacttttt
    57781 gtcgttttct ttcctgtatt cttattgaca gcaacgctta aaaattgaaa ggggcaaaac
    57841 agatgagtaa aaaattgacc gtccaagata tgattttaac attacaaaaa ttttggtcat
    57901 caaacggctg catgctgatg caagcttatg acacagaaaa aggagcaggt acgatgagcc
    57961 catatacatt cttacgggca atcggaccgg aaccatggaa tgctgcttat gtagagccat
    58021 cccgccgacc agctgacggg cgttatggag aaaacccaaa ccgtttgtat cagcatcatc
    58081 aatttcaagt cgtaatgaaa ccttcacctg aaaatatcca agagctttat ttagaaagct
    58141 tgcgtctttt aggaattgat ccattagaac acgatattcg ttttgttgaa gacaactggg
    58201 agaacccatc gatggggtgt gccggtgtgg gttgggaagt atggcttgat gggatggaga
    58261 tcacacaatt cacgtacttc cagcaagtcg gaggactacc ttgtaaacca gttacttctg
    58321 agatcactta tggactagaa cgtcttgctt catatattca agaagttgaa agtgtctatg
    58381 atctggaatg ggctgatggt gtgaaatatg gcgagatctt caaacaacca gaatatgaac
    58441 attccaaata ttcttttgaa gtaagtgacc aagacttgct actaagcaac tttgaccgtt
    58501 ttgaaaaaga agctaaacgc tgtatcgatg aaaatcttgt tcatccagcg tatgattata
    58561 ttttgaaatg cagccataca ttcaatttat tagatgctcg cggtgcagta tccgtaacag
    58621 aacgagcagg ttatctttcc cgtatccgca acatggctcg tgctgtagcc aaaatctttg
    58681 tggcagaacg cgaaaaatta ggattccctt tattgaataa aaacacagca aatccagaaa
    58741 acatgaagga ggctgaataa gatggcaaaa gacttattac ttgaaatcgg gctggaagaa
    58801 atgcctgctc atgttgtcac accaagcatg aaacaattag aagaaaaaac agcaaaattt
    58861 ttagatgaaa accaattgac ttatgatacg atcgaaacgt tttctacacc gcgccgctta
    58921 gcaatcaaag taacaaacat tccagaacgt caagcagaca gtgaagaaga agtcaaagga
    58981 ccagccaaaa aaattgcttt ggacgcagat ggaaactgga cgaaagcagc tgaaggattt
    59041 gtccgcggac aaggtctgac gacagatgct atcactttta gagaattaaa aggcgtggaa
    59101 tatgtctatg taacgaaata cactcacggc aaagaagcga aagaagtatt gacaggttta
    59161 gcagatgtca ttaaaagcct cactttccca gtaacgatgc attgggcaga atacgatttt
    59221 gaatatatcc gccctattca ctggatcgtg gcattgttag atgacgaagt aatcccgttt
    59281 gagatcttag atgtcaaaac aggcagagcc tctcgaggac atcgtttctt aggcgaagat
    59341 gtgacatttg cgcatgcaaa agaatatgaa gaaaaattga atgaacaatt cgttattgct
    59401 tcaccgcaaa aaagacaaga aatgatcgtt acacaagcaa atgaattagc cgaaaaaaat
    59461 cactgggtta ttgatctaga cgaagattta ctagaagaag tcaacaattt agtcgaatat
    59521 ccaactgtct tttctggcga cttcgaagaa aaatatcttt ctgttcctga agaagtacta
    59581 gtcacttcta tgaaagaaca tcaacgttat ttcgaggtac ggaacgatca aggtatgctg
    59641 cttcctcatt ttatttctgc tcgtaatggt gatcgggtaa aattggaaaa tgtggttcga
    59701 ggcaatcaaa aagtattgac ggctcgttta gaagatgcgg aattctttta taatgaagac
    59761 aagaaagctt caatcgacca atacgttgaa aaactaaaaa ctgttacttt ccatgaaaaa
    59821 atcggtagta tctatgagaa aatgcagcgg gtacaagtca ttgcccgtat cattggcgaa
    59881 caagtaggac tatctgagga agaattgatg gatctgcaac gagctgctga aatctataaa
    59941 tttgatctag taaccaacat ggtaggggaa tttcctgaac tacaagggat catgggcgaa
    60001 aaatatgctc tgctgaaagg cgagaaacct gctgtagcaa cagcgattcg tgagcattac
    60061 atgcctatat ccagtgaagg agagctacct cagtctacag taggttctgt tttagcgatt
    60121 gcagataaat tagatagcat tttttctttc tttgcagttg ggatgattcc aagcgggtca
    60181 aacgatcctt atgccttacg tcgccaagca tacggtgtcg ttcggatcat cgaatcgaaa
    60241 aaatgggcct ttcctttgtc tgttctgcaa gaaacgatta gtgatgttat cagtaaagat
    60301 acggatcgtt tcggcattgg tttatcagaa gggcaacaac aggtgatcga ctttatcaaa
    60361 ggccgtctgc gccaattatt gacaacgaag aatattcgtc atgatgtcat cgaagctgtt
    60421 ttgaatgcag aacaaaaaga tctgacaaaa gtatttgcag cagcacaatt gttcaagcag
    60481 catcttgcag atgaagactt caaaccatcg atggaagctt tgacacgtgt cgtgaactta
    60541 gcgaaaaaag cggagcttga aaaacaatca gaagtagacc ctgaactgtt cgaaaatgaa
    60601 gcggagaaag aactgcacaa agcagtagag gcaatgaaaa aaagctttgc tgaccaaacg
    60661 atcaacgaaa attatgatca gcttgtaagc cttcgcccac tgatcgaaaa ttattttgat
    60721 cagacgatgg tcatggcaga agaaccagca gtacgtaaga accgattaac gcaattaagt
    60781 caaattgctg ccatggcttt gtcgctagca agtttagatc aaatcatcac aaaataattt
    60841 ctaatatgta aataaaccag agaataggca caaaa
//
LOCUS       DARBFO010000008        57211 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000008 DARBFO010000000
VERSION     DARBFO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.008"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..160)
                     /locus_tag="SCK51_000633"
     CDS             complement(<1..160)
                     /locus_tag="SCK51_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEG1251282.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNI"
     gene            854..1240
                     /locus_tag="SCK51_000634"
     CDS             854..1240
                     /locus_tag="SCK51_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1251283.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1334..1525
                     /locus_tag="SCK51_000635"
     CDS             1334..1525
                     /locus_tag="SCK51_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1251284.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            2039..2371
                     /locus_tag="SCK51_000636"
     CDS             2039..2371
                     /locus_tag="SCK51_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1251285.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2281..2475
                     /locus_tag="SCK51_000637"
     CDS             2281..2475
                     /locus_tag="SCK51_000637"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1251286.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            2667..2822
                     /locus_tag="SCK51_000638"
     CDS             2667..2822
                     /locus_tag="SCK51_000638"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEG1251287.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            3098..4438
                     /locus_tag="SCK51_000639"
                     /pseudo
     CDS             3098..4438
                     /locus_tag="SCK51_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4679..6295)
                     /locus_tag="SCK51_000640"
     CDS             complement(4679..6295)
                     /locus_tag="SCK51_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEG1251288.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            6413..6631
                     /locus_tag="SCK51_000641"
     CDS             6413..6631
                     /locus_tag="SCK51_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1251289.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            6826..7287
                     /locus_tag="SCK51_000642"
     CDS             6826..7287
                     /locus_tag="SCK51_000642"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1251290.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(7480..7719)
                     /locus_tag="SCK51_000643"
     CDS             complement(7480..7719)
                     /locus_tag="SCK51_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1251291.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7743..9266)
                     /locus_tag="SCK51_000644"
     CDS             complement(7743..9266)
                     /locus_tag="SCK51_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEG1251292.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(9284..9406)
                     /locus_tag="SCK51_000645"
     CDS             complement(9284..9406)
                     /locus_tag="SCK51_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEG1251293.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(9436..10419)
                     /locus_tag="SCK51_000646"
     CDS             complement(9436..10419)
                     /locus_tag="SCK51_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG1251294.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(10443..10592)
                     /gene="rpmG"
                     /locus_tag="SCK51_000647"
     CDS             complement(10443..10592)
                     /gene="rpmG"
                     /locus_tag="SCK51_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG1251295.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(10613..10990)
                     /locus_tag="SCK51_000648"
     CDS             complement(10613..10990)
                     /locus_tag="SCK51_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251296.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(11022..11201)
                     /gene="rpmF"
                     /locus_tag="SCK51_000649"
     CDS             complement(11022..11201)
                     /gene="rpmF"
                     /locus_tag="SCK51_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEG1251297.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(11216..11485)
                     /gene="rpsN"
                     /locus_tag="SCK51_000650"
     CDS             complement(11216..11485)
                     /gene="rpsN"
                     /locus_tag="SCK51_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEG1251298.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(12008..13774)
                     /locus_tag="SCK51_000651"
     CDS             complement(12008..13774)
                     /locus_tag="SCK51_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1251299.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(13734..15487)
                     /locus_tag="SCK51_000652"
                     /pseudo
     CDS             complement(13734..15487)
                     /locus_tag="SCK51_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(15597..16166)
                     /locus_tag="SCK51_000653"
     CDS             complement(15597..16166)
                     /locus_tag="SCK51_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEG1251300.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(16184..17599)
                     /locus_tag="SCK51_000654"
     CDS             complement(16184..17599)
                     /locus_tag="SCK51_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1251301.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(17610..18278)
                     /locus_tag="SCK51_000655"
     CDS             complement(17610..18278)
                     /locus_tag="SCK51_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEG1251302.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(18408..18992)
                     /locus_tag="SCK51_000656"
     CDS             complement(18408..18992)
                     /locus_tag="SCK51_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1251303.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(19323..19556)
                     /locus_tag="SCK51_000657"
     CDS             complement(19323..19556)
                     /locus_tag="SCK51_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEG1251304.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(19571..20521)
                     /locus_tag="SCK51_000658"
     CDS             complement(19571..20521)
                     /locus_tag="SCK51_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEG1251305.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(20518..21360)
                     /locus_tag="SCK51_000659"
     CDS             complement(20518..21360)
                     /locus_tag="SCK51_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG1251306.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(21362..22081)
                     /locus_tag="SCK51_000660"
     CDS             complement(21362..22081)
                     /locus_tag="SCK51_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG1251307.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            22773..22931
                     /locus_tag="SCK51_000661"
     CDS             22773..22931
                     /locus_tag="SCK51_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1251308.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            23003..24229
                     /locus_tag="SCK51_000662"
     CDS             23003..24229
                     /locus_tag="SCK51_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1251309.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(24317..24709)
                     /gene="rpsI"
                     /locus_tag="SCK51_000663"
     CDS             complement(24317..24709)
                     /gene="rpsI"
                     /locus_tag="SCK51_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEG1251310.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(24725..25168)
                     /gene="rplM"
                     /locus_tag="SCK51_000664"
     CDS             complement(24725..25168)
                     /gene="rplM"
                     /locus_tag="SCK51_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEG1251311.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(25687..26154)
                     /locus_tag="SCK51_000665"
     CDS             complement(25687..26154)
                     /locus_tag="SCK51_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEG1251312.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(26274..27812)
                     /locus_tag="SCK51_000666"
     CDS             complement(26274..27812)
                     /locus_tag="SCK51_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEG1251313.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            27892..28578
                     /locus_tag="SCK51_000667"
     CDS             27892..28578
                     /locus_tag="SCK51_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEG1251314.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(28779..29306)
                     /gene="msrA"
                     /locus_tag="SCK51_000668"
     CDS             complement(28779..29306)
                     /gene="msrA"
                     /locus_tag="SCK51_000668"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG1251315.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(29390..30067)
                     /gene="pgmB"
                     /locus_tag="SCK51_000669"
     CDS             complement(29390..30067)
                     /gene="pgmB"
                     /locus_tag="SCK51_000669"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEG1251316.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(30064..31194)
                     /locus_tag="SCK51_000670"
     CDS             complement(30064..31194)
                     /locus_tag="SCK51_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG1251317.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(31194..33377)
                     /locus_tag="SCK51_000671"
     CDS             complement(31194..33377)
                     /locus_tag="SCK51_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEG1251318.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(33374..34399)
                     /locus_tag="SCK51_000672"
     CDS             complement(33374..34399)
                     /locus_tag="SCK51_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1251319.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(34444..35271)
                     /locus_tag="SCK51_000673"
     CDS             complement(34444..35271)
                     /locus_tag="SCK51_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG1251320.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(35268..36335)
                     /locus_tag="SCK51_000674"
     CDS             complement(35268..36335)
                     /locus_tag="SCK51_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEG1251321.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(36361..37197)
                     /locus_tag="SCK51_000675"
     CDS             complement(36361..37197)
                     /locus_tag="SCK51_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1251322.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(37194..38063)
                     /locus_tag="SCK51_000676"
     CDS             complement(37194..38063)
                     /locus_tag="SCK51_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1251323.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(38133..39416)
                     /locus_tag="SCK51_000677"
     CDS             complement(38133..39416)
                     /locus_tag="SCK51_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEG1251324.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(39430..41115)
                     /locus_tag="SCK51_000678"
     CDS             complement(39430..41115)
                     /locus_tag="SCK51_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEG1251325.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            41390..42415
                     /locus_tag="SCK51_000679"
     CDS             41390..42415
                     /locus_tag="SCK51_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1251326.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(43010..46684)
                     /gene="rpoC"
                     /locus_tag="SCK51_000680"
     CDS             complement(43010..46684)
                     /gene="rpoC"
                     /locus_tag="SCK51_000680"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEG1251327.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(46742..50359)
                     /gene="rpoB"
                     /locus_tag="SCK51_000681"
     CDS             complement(46742..50359)
                     /gene="rpoB"
                     /locus_tag="SCK51_000681"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEG1251328.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            51087..52055
                     /locus_tag="SCK51_000682"
     CDS             51087..52055
                     /locus_tag="SCK51_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1251329.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            52073..52978
                     /locus_tag="SCK51_000683"
     CDS             52073..52978
                     /locus_tag="SCK51_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1251330.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            52975..53781
                     /locus_tag="SCK51_000684"
     CDS             52975..53781
                     /locus_tag="SCK51_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1251331.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            53917..54906
                     /locus_tag="SCK51_000685"
     CDS             53917..54906
                     /locus_tag="SCK51_000685"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEG1251332.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(55223..55747)
                     /locus_tag="SCK51_000686"
     CDS             complement(55223..55747)
                     /locus_tag="SCK51_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEG1251333.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            56034..>57211
                     /gene="scm"
                     /locus_tag="SCK51_000687"
     CDS             56034..>57211
                     /gene="scm"
                     /locus_tag="SCK51_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEG1251334.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     S"
BASE COUNT        17337 a        11556 c         9712 g        18606 t
ORIGIN      
        1 taatattatc agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc
       61 gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa
      121 catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt
      181 tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa
      241 gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa
      301 agtacatttt atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata
      361 tctagttcaa agacaataat tattatgatc cgttattatc taagcactat acagataata
      421 ggaatttttc gtttctaaat attccaattt gggaatccaa taaaaatttt cgttcaaata
      481 cgtgtctatt cgaactcttt tctagttgtt aattatgaag ggggaagaaa taaaaaatac
      541 aatctataca cgaataagaa tattatccaa aaggaggttt tagaaaagat ttattatacg
      601 taaaaagctc aagacatttt cttagccata aggacatgtt tgatatttaa gaagatatat
      661 gaattttctt ttctaagtat aaagataact atccattatc aactaaaaaa acatttttga
      721 aaataaacat cagcagtcct atcaatgcat gtgagcttat agaagttatt cgcagcgcag
      781 cataattgca ttagcttctt ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga
      841 ggaggtgtgc aatatgtcag ataacgacga acgcgtaaat gcccagttga ttcgattctt
      901 tgaaaaggtc attttgaaca ccgctaatac ttattataaa aaacaagaag aaattcaaaa
      961 acatgaacaa cttgatcaac tcccaccata tttgtttact aaagatacta taaaatttaa
     1021 acaaccgatt aatgttttaa atgtgactct ccttgttgaa gatataaaat tagcaaaaat
     1081 ccttcctttg ttgaaagaac ctgaacaaat ttttcttatc gaaaaattta tctttgataa
     1141 aactgataaa gaaattggaa aggttctggg gattacaaga cagggtgcga caaatttaaa
     1201 acatcgactt tacaaaaaaa ttcaaaaaat gctggtttaa tatctgcata ataactaatt
     1261 ttgtatgctt ctttgctcgt tctcctcctt cccaaagaaa tagattataa gtccatggga
     1321 agaaggtaga tttatgaatt ctttaaaaga agatttcatt ttagcaaagg ctggaaatga
     1381 agaagctgtt gaggcaattc tcaaaagatt tagctctttg attcataagc aatcctggag
     1441 aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat atctagctat
     1501 ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata agtaaaaaga
     1561 tagtttcaaa tttcgaacaa taaatttgaa actatcttct ttataattat aaagtatcga
     1621 ttttacagcg ttctataatg attcatagaa tctaaaacga atgattttaa ttatcaacgt
     1681 tttttaattc gtttttagct taaaaataac ttttgtcaca gtctcactcc taaaattact
     1741 ttatttacgt atgaagtggg caggaggtcg aatccagaac tgggctatgg tacttcaacg
     1801 aggacttctc ctctagaatt gaaaagcatg cgatttacct tccgtaacga cggtagattt
     1861 attcaactat gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag agaattttgg
     1921 ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt gacaaaccct gatactaagt
     1981 tgggctatga tacacttttc ttgactatca gttacgttac cggaaggaat gaactctatt
     2041 gagaaaaaca tatgatcgta aatttaaact aaagatttca caggatatcc ttgagaaaaa
     2101 agtaactacc aagaaaattg ctgaagaata caatatctct cgtccaacaa tttcccgatg
     2161 ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa aaagattacc
     2221 tgataaagca gatttctaca ttcttgaaca ggaaaataaa cgactaaaag aggaagacga
     2281 gtgttctacg aacacaaaga tcgatacgga tctccaggga tcactcaaaa actttacgat
     2341 gaaggaatcg aaatcaataa acgtgttgta gctgttctta tgcgtgaaat gaatttatgc
     2401 acaagaggat ttcatcgacg caaatcttct catggtagag aaaaaacaat caaagaaatg
     2461 gtaaaaaaat gttgaatcga taatttgaac agaaccaaat tgatgctgtt tgggtgatgg
     2521 atattactta cattccatgt aatgacggtc gcctctattt gtccacctat attgacttag
     2581 caactcgtat tctacgttac tatatggtag attcacacat gaaaaaagag attgtcgtcc
     2641 gtccattaca aatttattaa ggagaattgc ctacggtaat tcattccgat agaggtagcc
     2701 agcatcggtc ttttatttat caagagctgt tgaacgacca ccacatcaca cattcagtga
     2761 gccaaactgg gactcctgtt gacaatgcag taattgaagc gtttcatcga tcaatcaaat
     2821 gagaattaat cgactccaac aaatataaaa gtaaaataga gatgaaggtg ttaatccaag
     2881 attatttaga agactactac ccaaacaaaa gaatccatac aaaatttatg atgactcctt
     2941 gacaatttga agcgaagaaa ataagggatc tataatatct agtgttggtg acgccttctg
     3001 ttgccaatac tttttctttt ttctaataaa cgcaaacaga caccttaaaa tcctttagaa
     3061 taaagtcgac gaaaaccatt caaaggagtg tttcaagatg tcctattcag agtttaccaa
     3121 agagttacta gatattctag acctaaatct tatctttcat gaagatgcct ttcgcaaaga
     3181 gcgaatcaat gacgaaacat gttttgtttt tgatggcaca ttgacctatc aaccggagga
     3241 atgctttcat cgccactatc aaaatgaaca aacgatcatt aaatagggct ggaaaaaagt
     3301 atccatctta ttgaatgatg tcagtaacta caagaccatt ctgcggatta acaaacagcg
     3361 cttcaaatgt aaacactgtg gaaagacttt tttagcagaa gattcagtta gtgatcgtcg
     3421 ctgctccatt gctcgcagag tcaaacaagc cattcttgag ctattaagcg aacctctttc
     3481 tatgtctttg atcgcacgta tgaagcacat ttctccgacc actgtcattc gaatacttcg
     3541 cagccttcgg cctaaaacca tttctttgag ccagctgtta ccagaagtgg tgtgtttcga
     3601 tgaattcaaa tcagtgaaaa atgtctcggg agccatgagc tttgtcatga tggacggcaa
     3661 gacccatcaa ctgattgata ttgtggaaaa tcggcaactc aatcctttac gcgactattt
     3721 cttgcgctac cctataaaag tccgtgaaca agtgcgtcta gtggtttcag atttctatac
     3781 accctatcgc acattggtga aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt
     3841 tcatattagc caacacattg gtcgggcttt taccaaccac cgaattcaag taatgaagac
     3901 ctttaaaaaa ggggatcgtc ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa
     3961 gaatgcgtgg gaattgaaag gtcagcatcg ctactggcgt ccgtcatttc gagatcatct
     4021 gacagaagca gagatagtcg atcggctatt atcttatgat gattccttaa agcgaggcta
     4081 cgaagtctac caggattttc tttccgttat cagaagacaa gatgttcctg aattcgtcgc
     4141 gctcctaaaa gaagactaca aggagttgcc agagcactac caaccagtgt tcacgacctt
     4201 caagaaatac caaacagaaa tcaaacgagc gttgcgcgtt ccctattcca acggccctat
     4261 agaatgtttg aataaccata tcaaagtact aaaacgaatt gcctatggct atagaaactt
     4321 tcaaaactac cgagaacgaa tctttttata tcgaggaaaa tattttaaga aaactaagaa
     4381 tactacccaa ctgactaaag ccagaacaac aacacggcta gacaagatag ctgtctagcc
     4441 gtgtgagtat ctcgattatt ctattgaatt tcttcaccaa cactcattga cgaagagcca
     4501 aaataagtaa tctaaccgac tgttaactaa accattgcag gtcccgttaa agatacttgt
     4561 gcctgttgtc accgcttctt tttcttgtcc acaagcgctt aacacactta ataaagcccc
     4621 tgctattatt aataataata tctttttctt attaaaaacc tcctcatcta tttccagatt
     4681 agtaaatgta gtaatctgtg ctttcttttt tctcaatact aataactaac ttatggggta
     4741 aacaaatact cgtttgaccg ggttttgaga tccatccagt tctaaccgcg atttgatcag
     4801 gactattatc ttctttaact cgaattcttc catctttaat ctcaacaata ttatattgtc
     4861 ccttcgcagg atgataattc acgagtttat gattgatctt atccaaatcg aagcgatcca
     4921 tttccttgcc atcaattcga acgatggcat atttgatagc atcttcagtt gtttcattct
     4981 ccttttctat agagaataca aataagggaa taaaagaggt aataatcaaa atgccaataa
     5041 ttatgacatc gcctatcttg acaccagctt ttcttaaaaa tttcattaac gatatctggc
     5101 taaacatatt agactttttc catggaaatg gtgaaatttt tcggaaatta tctgcattaa
     5161 gagaatcagt taaacaaccc tcaagttgtt ccttaataca ttgttgatcc atcttcatac
     5221 caataaaaac tagtttatta atgcgttcac caaacaacgg atgccaggct tccttcaatt
     5281 cgggattttt ttgtataatc atttgacact caattttagg taaactagct acccaataat
     5341 ttaatgattc aatactttta tagctacccg atgtttcaaa gatcgtcgaa aaatcactat
     5401 gttgggcaaa ccaaagaaac ccttttgctc ttgtaatatt gccaggaaaa tttttatccg
     5461 taagatgggc aaatttttgt gcgtcaagga gtaaccgatt tcgatagata aaacttgaga
     5521 taccatattc ttctgtttca ggaatatgat tttgatagcc aagctctaac tctttgaacc
     5581 agcccactga ataggaagtc tcatctatat taaaaagatt tgtatttaga attttcgata
     5641 agtctacttc acctttaatt gtcctaatta ttttggcatt ggactgtaac ttagcaatta
     5701 gaccgttaat tttgtaaatc gtcttggttg acactaagtc actattattt aaaagtaata
     5761 tgttacaaca ctcgaattga tcaattaaca agccacgaat atcctgttct tcttcaacta
     5821 cattaccttc agcttttctt tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac
     5881 gttttacatc cactactata ataatggcac caatctctaa tcgttgggtc aaatcaatgt
     5941 ctaaagggtt tttagctaat ataattgctt gaataattgg aattggtttg ctgattcctg
     6001 aagcttcaat aacaatttga ttcaagtcag aaaattgcac taattgatct agttcaacaa
     6061 tcaaatcttc gcgtaatgta caacaaatgc atccattcgg catcgaaaca agtttttctc
     6121 cagttctttt aaaacctttc ttgtcgacta ataccccatc aacatttact tcccctatgt
     6181 catttacaat gacagcaatt tcctttccgt gatcaccgta cagaatatga tttagaagta
     6241 tcgtttttcc tgaaccaaga tagccagtta agattgtaat aggtattttt ttcataggga
     6301 acctctttct ataccttgaa tttacacttt aagtgcaaca cataaaaatg agtcataatc
     6361 gatatccaac tagtataagt ttgtcgagaa cactcctaga aggaaaataa ttatgagcca
     6421 gtatcgtcat cttactatac tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt
     6481 ttctattagt ataattggca acgacattca gcatagcccg tttactattt tacatgaact
     6541 gagaagaaac aagaaaaaaa ctattcacct tccaaagcgg aaaaaaatta tgcttatcga
     6601 agaaaaaaat gcggacgtta tcacattcta aatgatccgt gagtttggtc aattgttcgt
     6661 catttatttg tcgattttca gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa
     6721 catatggact tttcaattag ttttatcacg atctaacgtg caatttgcaa tgatcttttc
     6781 gatacagaac ccctctcaca cggcaataaa ggcctgatcc gttcattgca ccatcgagga
     6841 aaaacaagac atacaaaaaa tcatgtcgag tctcgtggaa agatttgcct tactcattcg
     6901 atccatggcc gtccaaaatc cgcagatgat agaagtgatt ttggtcattg gaaagccgat
     6961 attgtagctg gcaaaactgg tggtacttgt ttagtgactt tgacagacag aaaatcatgc
     7021 ttccttttag caggaaagaa ggtccccaga aagaaagccg cttatgtgag cgagaaaatg
     7081 attgaacttt taagcagtat tccaaatgag gcggtcaaaa cgatcactcc agatagagga
     7141 aaagaattct ccaatcattt cgcagttaca gaagcattga ataatatgcc cttttatttt
     7201 ccagatcctc atgcccttgg caacaatgaa caaattaaaa tacgaataga ctcattcgag
     7261 aatacttgcc aaaatcgaaa gagatagcta cggtagataa ctcaacgata aatcagatga
     7321 ttgaaaaact gaataacaga cctagaaagt gtttagattg gagaacacct tatgaagtct
     7381 tctttgataa agtgttgcgc ttgatttgac aattcaagac ataataaaaa gcagatcaaa
     7441 tccaaataag ggaaaaatga tctactctgg tattatcaat taattggcca agctattatg
     7501 ccgttttgct aaacgtctac ttcttccagt attgaccttg ataaaacaac aatcaggatc
     7561 ccgttttaat actctatgaa ctgtgtcaat actaacatgt aaagttgcag caatatcagc
     7621 taatgtactc ccatttaatc tcatttgctt tgcaaatcga taacgccgat aagcatcaat
     7681 attcttggaa tccatagaca ataatacttt tcttaccatg tatttcctcc aaaaattgaa
     7741 ttttaatgag ccatcatttc ttgagcaatt tcatgtttag acaatgaagc tggatagaaa
     7801 gtaggccaat tatgcaattc attatagagt ttttcttgac tttctccacc aaagaaaata
     7861 tggaaatgct ctgccttact tggtgcaatt ccatgatcac taaattgaat atatttaaaa
     7921 cgaccagcac ctgcatcatc agtttcaaaa ttaaaacgaa caccacggtt tcctttttca
     7981 taattcaaaa ttttgtatcc aacatatttg tatgtgtatt gatgatgttc accgttgact
     8041 acaaaatcaa tggtgttgtc tttaatgttg atagtatcaa tatctgtctt atagccagta
     8101 gtataataat ctttgtactc ggcagctgtc atgtctttat tgatttttgc tttgtaatcg
     8161 aaaacttcat ccaaaattcc atcttttaac aacggataaa cagattgcca ttcgcctgta
     8221 taatcactca attcgcgatc cttcacatcg gcatctttaa agtacccatt agcaactgtc
     8281 ttttcatcct tcgcttcttt ttctggaact acctcttcac cagcaaccat agttgttttt
     8341 tctaacgctt ttagattgtc ttccattact gaaacataat cttcaccatt atccatttgt
     8401 tcttttgtaa gactttcaag aggattcagt acttccaatt ttatcccagc ttcgttggcc
     8461 aaagtttttg caattttatc attagcattc ttttcaaagt aaatataatt gatcttattt
     8521 tttttaacat actcttttaa ttcagctaat cgaccagaag atggctcttc ttcaggactt
     8581 agtccagcaa taggaacttg tttcaatcca taatccaaag ctaaatatcc aaaggctgcg
     8641 tgttgcgtca caaaactttt ttgtttcgct tctttaagac tagtggtata gtcagcatcc
     8701 aaccgtttca gtttagtcaa atacttttca gcatttgtct caaatacttt tgcttttttc
     8761 ggatacaatt taactaactg atccttgata tttgaaactt ccttaattgc cattttgggt
     8821 gaaacccacg tatgaggatc tagttcatga tggtgatctt cttctccgtg atcatgatct
     8881 tcttcttcac tacctggcaa taaaatcatg tcttttgttc cttctactac attaggccca
     8941 gctttcttcc aactttcttt agcttttggt acccatgatt ccatgttttc gttatgatat
     9001 acaaaaacat cagcatcatg agcttttgcc atatccttag ctgagggctc atagtcatga
     9061 ggttctgatc ccgctggaac cattaaatcc acgttcgctt catcgccgac gatgtttttg
     9121 gtgaaatcat acattggata gaatgttgtt acaatttcta attttccatc tttatcattc
     9181 gatacttttg catcattagt gctggagcaa gctcccaata atcctagagc tgctatccca
     9241 ataagtccta cactgatttt cttcattctt tttccttcct taattatgct aatttcttca
     9301 ttttatttcg tttatgttct ttgataattt ttaatgccct ttttctagtt ttaatattcg
     9361 ggcttttaag tcgtcgatat gcactagata tatccgtttt ctccattaga aattaccccc
     9421 aatcactact tatcattatt tgctttccgc cagtaattta tcaaaactct gtttgatcct
     9481 ttcttcatcc aacatttttc cgattagaat aatagtggtt tggtcttttt gtcgagtggt
     9541 gtattgaaaa ttaacctgtt cgtttacctc ttgcatggca actataaaat cctgctcttg
     9601 taaagcaatc aatcctttaa aacgatacag gctcccttgg ttggttaaaa tcagccagtc
     9661 aatccattgg agtaaaaaat ctttttttat gtcttgatct gaagttaaga gaattgattt
     9721 aaactcatgt acttgatggt gttgatgata gtatttgcca ttatagtgat tttctgatga
     9781 atcactttca aaaactaagg gatgattaaa ttttttcaaa ttataaaaat cctttttttg
     9841 aatcgtttca ctatgtgtga acattcgaat atcggataaa gggttaatac ttttgatttc
     9901 tctctgtaat ttttttagtg cagataaatt caagttttgt tttctagaca caaaaatgcg
     9961 atctgccatg acaagttgct taagggcttg tggttctctt tcaataagtt caaaactgtc
    10021 actatcaata acagtcaata aactatcgat atatactctt cctcttagtt gaggcgctgt
    10081 taaaagtgtt tgaatgattg gttggggatc tgctatacct gtagtttcaa tgatgatttg
    10141 tgaaattggt tgttgatgtt cttctattgt ttccagtaaa gaaatcaata ttgctaataa
    10201 atcactgcga agactgcaac acatacatcc attatttagt tgtaacaccc gttcctcgct
    10261 atgaactaat aatttgtgat caatcccaac ttcaccaaac tcattttcaa ttataataat
    10321 ttcttccctg gggtagggtg agacttgcaa ggcttgattg attagcgttg tttttccagc
    10381 ccctaaaaac ccagaaacga ttgtaactgg tatgcccatt ttgtcacctt tttaccactt
    10441 agctatttca cttctttaaa aattgcccgt ctgcgcaatt ttggcgaata cttttttaat
    10501 tctaaccgtt cagggttatt ccgtttattt ttgctggtga gatacagacg ttctccagtt
    10561 tcgacacatt ccaaaataat attttgacgc ataaatcttc ctccttcaat acttatcgat
    10621 cttcaagata tgcttgaatt tgttgtgcat cttcaaaagt taactcgcca gattccaatt
    10681 gtgaaactaa attttctttc atatcaacag gtatctccca ataaccatcg atacattgaa
    10741 ataactcctc gtcatactct tcaaaaattg gatagattct taaaaaatca tcaaatgata
    10801 agccttggtc ctcatagtaa tctttgacta atccattttc aattgcctgt tgggtcggta
    10861 aacccgtttc agtaataatt cctctctcta ccatcgaacg ataaaaagtg catttatcta
    10921 gcgattgctg ggtcgtttca agataatact ttaatccttc ttcaactaaa ggtgggtaaa
    10981 taatttccat tttgattccc tcctaaaagt tatatattaa actattttga agctccttta
    11041 attactttct ttccattata gtaccctttt aaagatacat gatggctacg tctataatca
    11101 ccattcgctt cgtcaaatga aatttctggt tttactacag tttgatgagt tcttcttaac
    11161 cgtttcttcg ccttagatgt ttttcttgct ggtactgcca tgatatttcc tccttttacc
    11221 aacttgcttt ttttactcca ggaatcaacc cttgatgggc aagttctcta aacttaatac
    11281 gggacatacc gaactttcgc atataccctc ttggacgacc atccgtctga tcacgaagcc
    11341 tcagacgatt cggatttgaa tctcttggca atttagcgag agcggtgcga tctcctgcgg
    11401 cttttaattc ctgacgttta tctgcatact tttcaatcat cgccatttgt ttctttgctt
    11461 tagcaatttt cgactttttt gccatatacc tttatactcc ttttattttg atttagacac
    11521 ctcaaatcgt aatcgttacg ttttgacact gatactttac caacaagagt tgctcttgtc
    11581 aacaataaat aacatgaaat aaaactcata tgaatagata actatttgaa aacgaattta
    11641 tcggtacata gagataatca aatcatttaa aagaatatgc agtttaggct ctgaaaaata
    11701 agaattcact tttattagtc caatcaaaca caaataccgt tttaagtagc ttttcctcta
    11761 cttaaaacgg caatatgaaa gaaactataa ctttttattc aatcttttat gtttaactgg
    11821 tttatcgcca attttttaaa actttgatta ttccttatat tataagaata gacattaagt
    11881 gaactaattc aatcatactg attctttcgt gtattcaaaa ttagatgctg tttaataaaa
    11941 ttattctttt tgaaaaagaa ttataactat atcccaaaaa ttgtaggtta atttaaagcc
    12001 tatttcatca tcgtgattga gcttcttttt catagacgaa tttctcagat agtttagttt
    12061 tctttatgag atttttgtat atttctgacc ttcgatacaa ctcattatga gtaccttgat
    12121 caactagttt tccttgatcc atcactacaa ttttatttac cttttcaata gacttcaagc
    12181 gatgcgatat aattagtact gtcttatctt gtattaaacg atttaaactt tcctgaatac
    12241 gtttttcatt ttccgcatct aaactggcag taatttcatc taaaattaaa attggcgcat
    12301 ttttcaaaaa agcccgtgct atggataggc gttgtcgctc tcctccagaa agtgaacttc
    12361 cgttttctcc gaccaatgta tgataaccgt caggtagttt ttcaataaag tcttcgcagt
    12421 tagctaaacg agctgctttt ttcacttcct catctgacgc ttgactattt ccaatacgta
    12481 tattttccaa aatacttgta ttaaacaaag ttacctcttg aaaaacaata gcgattttgc
    12541 taaacaaaga agctggagat acacgtttaa tatcataccc atcaattaaa atacagcctt
    12601 catcataatc atacagtcta gacactaatt tcaagatact agttttcccg gaaccactag
    12661 cacctactaa cgcagtcact tctccttgtt tagccgtaaa agagatatga tctaaaattg
    12721 gggtattgtt atcatatgag aaagagacat ctcgcaactc aatgtcaaaa gatttcagag
    12781 gactatctga tccctcttgg atactggttt ccttcatagc tctaattcgc tgtattttag
    12841 gagctaaata aaatatttca agcactgctt ctttcataga atcgaaggaa tcctttattt
    12901 ttattgcggc tagtaagtaa ccaacgacgt agagtatagt cacttcaccc gttaacagta
    12961 agtgaacacc tactagaatg actactgcta aactaacata tgagaaaata gaggataagg
    13021 ccatgactga aaaagtactt aattctacct ttaaatgaat cctttcactc tcttccattt
    13081 ttttgaaaag tctgtcttga acttttttag atagattaaa gctattaatc tcttgctgca
    13141 attctatagt ttcttgaaat tcttccgagt tttctctcaa agtatcgtaa tatttagtat
    13201 tggctttcgt ttgggatttc tttgataaca agattaagac aaaactaaat aacgttggca
    13261 aaataacagc taatcccatt ttgacattac caaccaaaag catcactgaa ataaaaggga
    13321 aaaacagagc cataccgccg gctttaggta ttgcatgact catcgcatgc tcaatacctt
    13381 caacgtcaga catgatagtt tgagataaat ctgataaatt atgttttgaa aaataggata
    13441 atggtaaatt tgacaaatcg tctgcaattt gcactcttaa atgcgcactt tctttataag
    13501 tactattata taagttttca tactccctat ctaacaacca atacatgaaa agcaaagtaa
    13561 caaacgatac ggcaaaataa agccaacggg gctttgtatt ttctaaaact aattcgtcta
    13621 tcagcatcat tataatgaat gctggtacca tattaatgca gtatacaaaa aaactagaaa
    13681 tagaagcttt agttaaagct tctgcgccct gatcacttaa agcaaatttg tttttataaa
    13741 aacttttcat tgtcaagcct ccaatcattt gcattctcat aattagtgac caattgttta
    13801 tacacttgat gtttagccat taattcatta tgatttccac gttctactat tttcccttct
    13861 gacaagacaa gaatttcatc tacagattta atgctggata atcgatgagc tatcataata
    13921 actgtttttt ctttcatcag atgttgaaaa gccttttgta gttcatactc attatctgga
    13981 tcaatagatg cactggcttc atccataata acaatctttg catcttttaa aattgctctg
    14041 gcaatagcta tacgttgttt ttctcctcct gacagataaa ctcctttact accaattaag
    14101 gtatgctctc tttcgggaaa tttttctaaa atcgattcgc aaccggctaa tcttagcgcg
    14161 tcaaatactt tacttgctgg cgcttgagga tttgctatag aaacgttatc gtaaatactc
    14221 tgattgaata gtttcgtatc ttgaaagaca aaagcaattg tgttaataat tgtgtctttt
    14281 gaatagtgat ttaaaggctt accaccaatt ttaatctgtc ccttatccac attgtagaaa
    14341 cctgaaatta gtttagctat cgtactcttt ccgcttcctg attttcctac taatgcataa
    14401 attctttttt gttctaaaac taagttgaaa ttttctaaaa cacactcttc tttgtatgaa
    14461 aagctgacat tttcaaatgc aatgtcacaa tttttaatct gtgtctcgtt cccatatttt
    14521 agttggtctt gcttcatttc taaataaagg gagtctagtg tttcgactgc atatcttgct
    14581 tcaaacaagt gcatacttac atacattatt ctcatgaaag aaacaaaaat aattccactt
    14641 aaaaaaaaca acatcaacaa gtcaacagct aatatcttgg gagtggtaag agaagttata
    14701 aaaaaggtta taggaactat caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga
    14761 acaaaaggtg ttttcccact caaagaatac ttatatgcaa acttagcata atcgttaatt
    14821 gctttattca aggctttaaa tgatgttact ttagcaccaa atatctttat aacttgaatt
    14881 ccgcggatat attctaccgt ttcagagctt aacacatcta acgattcttg atatttttga
    14941 ataaaatttg tattctctgt cattttcttt aacaagaata caccaattac taacatggcg
    15001 attagaacca atccaatttt aaagttaatg aaaaagccta aggcaactga taaaacaggt
    15061 gttactatgg cttgagcact atctggaatc aagtgagcaa ttgccatatg tgttttagcc
    15121 gcgttgtcat caattgtctt tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa
    15181 cttgcttctg ttaaaccgct aattcccttt tttctcaaat tagtctctaa gcgaaaccca
    15241 aggatatgag aaaataagcc agaaaaaata taaaataaag ctctaaagtt aaccaagcaa
    15301 cagtttgaac cgcataactc ttagctaaat attcatctcc tacaataatt agagactgaa
    15361 gaaatttgta aatactgtaa tatccataga ctgttaatac gacagaaaaa tcagaaaata
    15421 aagtggctaa ccatccgtag tatttcattt tagggacata actaaataat tttttgtata
    15481 ctttcatttt ttcctccccc gcattcaatt tcttctctta ttcgtttaca actgttaatg
    15541 ctttccgtaa tagcgtaaat tagagatgta caaacagata aaaggtaaac tacgttctat
    15601 tttttgtatt tttttaagac ttttcttcca atcaatacac caatcataga taaacttgct
    15661 acaactaaca ctgaaatgat tccccatata cctaccgcat agtggatcat tgcatccatt
    15721 gttgcagcag attgtccttt atctatccaa tcttgacgaa aacgatctgt aaagaaccat
    15781 ataggaatcc actgtccggc agcactgcct aattgcatta gagcccaaga cgcaacttga
    15841 ggaattgctc gatcatatcc aaatacagat agaataatat cagctaagat cgccatacta
    15901 atcgaactga taaaccaagg taagtacgcc ccgcccatta acgtaaaaaa taacaataaa
    15961 acaattgtgt aaatagtaaa aattcctttt ctagggtagt tatagctaat ataaacaaat
    16021 atgattccag ataataatcc aaaaatcccg ggggatatgc tgtgaccaaa cgccccacca
    16081 atactactaa ccattattga aaaaaagtaa attactaggg aaataactgt agctaaacca
    16141 ataaaaatat aatcttttgt tttcatatat attctcctca attctatttc gttatgtcat
    16201 taaaaatctg ttctatcaaa gttgaatctt ctactctaag ttttatatct ttttttattc
    16261 caccattttt taaataaata attctattgc atgttttata aagaaattcc ggatcatgag
    16321 tagctataac tactgcaccc ttttctttta agtctttaat aaggtcagca atcctttcca
    16381 tattaatata atccaaccca cttgtaggtt catcaaatag aaataaatta gtttttgaca
    16441 atgaagctat agaaaccaat aatctttgtt tttctccacc ggataaatca aacggtaatt
    16501 gatctttcat tttataaagt tccatttttt ttaaaatttt ttcttgctga tctttactaa
    16561 ctatttcatt ccccagggct aactcgctcc tcactgattc tgtgaaaaat tggtaatcca
    16621 tttcttgcat tactaaaaac ggcgatgaca aatttttttt attttcgatt atctttcctt
    16681 tggtagggcg aataatcttc atgagtattc ttagtaaggt tgttttcccc acaccgtttt
    16741 tccctaccaa accaataaca tctccaactt tgacatcaaa atttatgtct tttagaatat
    16801 tttcataagt aatttcttca acttttaata catctgcact taccttttcg atatagggaa
    16861 cttcaatcga actattaaaa atgtcaaatt tccttaatgc gttattgttt tcagttttta
    16921 acaactgaat agttgtagtt tttacagttc tatttttaac aaatagaact tgatcaatta
    16981 attctttcaa atagtaaaaa cgatgttcag ctataacaat agtaaatcct ttatttttta
    17041 aatcacaaag taagtcagca agtttcttag tcgctttgaa gtccaaattt gaagacggtt
    17101 catccaatat aattaattta gagtttagcg ataaactaca tccaatagaa attttctgcc
    17161 gttcaccact tgatagcgaa aaaatgtttt tatctaatat atttgaaaga tctaataatt
    17221 cagctaattc attgattctt ttatccatat gctcttttgt aaatccaaag ttttccatcg
    17281 aaaatactaa ctcactcgtt gtatttgttg taaagaattg tcctctagga tcttgactta
    17341 cgtagccaat attttttgat atactttctt gtgttaagga ttgtagcgag ttattttcaa
    17401 ataaaatttc cccaaccaat tttccgtcat aaagtcttgg aataagtcca ttcattaact
    17461 ttaatagtgt ggatttccca ctcccagaag aaccagtaat caatgtaaga ctgccttttt
    17521 ttattgaaaa attaaggtca acaagcgctt gttccttaac atccttatat gaaaaagaaa
    17581 cattgttaaa ttcaaacaat tctttcacct caaatcaaaa tacttaagaa aaaaatagta
    17641 acggaaaaca ggatgatata gtctaataag ctcatgtttg ttttatagta agaacttctt
    17701 ttcacaggag attctatccc ctttgttatt gcattagcgg tcatttcttc agcaatcata
    17761 gttgcacgta taagaacggg aactaacaga aattccaaat attgcagagg ctgaaaaaaa
    17821 gacaagcgaa tgtttctcat ttttaaagat tctttaatca ttcttatttc agaacgataa
    17881 gtcggaaaga atctgatcac tactgtaata cttaacatta tttttttcgg taaatgtatt
    17941 ttttctaaag aggataataa ttcattgcta gtatagacac taaccaacaa ccatgagatc
    18001 attacgaccg gcaacattcg aaatacaata tataaggatg tcgcaaaaaa tatagtaaaa
    18061 aactctatct tgatagcatc aataattttc attactaaaa acagaactga ataaatcaag
    18121 atgaaataaa tacatgtctt tattttacga aacatcaata ctaaaactgt agcaataatg
    18181 aaacacccca cttgaaatat atttcccttt cctaaaaaaa taatgaccgg aaaaaaaata
    18241 tttaaagtaa tcaaggttaa cggattgatt tttgacactc tttcacctcc aaaaaataag
    18301 atcgcactgt tatcttaagc tgataaaaat gcaccgttat tcagataaca gtgcgatctt
    18361 atcaaaaagc ataactgaga acaattctca atgcaagctt tttttaatta actcagtaat
    18421 tcaatccacc caggaacaga aaattgatac aatcttctgg ttaatagcat agtcttttct
    18481 tttgaatagc tatgcttcaa tatatcatag aatgtagcgt agaatgagta aatgtagaag
    18541 tggatttctt cttcattaaa gactaaattt tgattgatca ttccgatagt ctttaactta
    18601 tctagacaac agactgtctt tttcgtcaca aatgatgtaa ttttttcaac aatatcttga
    18661 tatttggttc cagctgattg gaataaaatc aaatcgaata tacgtttatt ctcaaaaata
    18721 taattaataa atggatctaa ttcattgaag ttctctctcc aacttttctc tatatctata
    18781 ttttctaagt ttaagaggta tttggtaaac tcttcattat acatattaat aagcgcatta
    18841 atatgcggct gaataatttc ttcaaataac gcttccttag aagtaaaatg tttataaaaa
    18901 gcacccgtag ttacgcctgc ggtcaggcac agttgcctta tcttagtttg ctgaaatcct
    18961 tctttttcaa aatggcttag tccactttct attaactttt ggtgagttat aatataatct
    19021 ctggtttcta tgggtacttc ctcctaataa gatgaattaa ctttattatc ttacacagga
    19081 cttgaagttt caatcgttaa cgtttattac tgaactcatt ccttagagct aaactaatga
    19141 gaaagagcag aataactgat acaaaaagca atcctacaag aattaataga ctcatctaaa
    19201 tcattcacct tttctaaaat aatccgagaa agatttaaaa aaattatatc cagctattag
    19261 gataagtcct atcataatca ctccaatagc cattttctta tcctccctaa aaataaagtc
    19321 ttttacttaa aacctttttt gtaatcgtca ataaattttt tccctttatc agttaattga
    19381 aatcctatat ctaaatagtc ctcatatcta agtttagcca gcatctcact tacagatgct
    19441 ggactaacac ttagaacttt tgctaaatca ttatttttta atttcttttt tctttttgat
    19501 aattcctgaa ttgctagtaa ataaattagt atagtagtcg ttttaatttg taccatacct
    19561 accctccaaa ttatttcgaa aggccttcag caattttatc caggttccat ttcatcatcg
    19621 catagtaact atcaccatct tgtccttttt ctgcaattga atcagtaaaa atcgttgaat
    19681 agataggaat attggtatct tttgatactg ttttcattgg tctatcatcc acactgcttt
    19741 ctacgaataa ggagggaact tttgttgtgc gtaatttttc aactaagtgt tttatttgat
    19801 ctggtgttcc ttcttcttca gtgttgattt cccaaatgta agcagaaggc acattatacg
    19861 ctttcgagaa atatttaaag catccttcac ttgtaacaat cattttttta tcattcggaa
    19921 ttgaagcaaa tttagattta gcttctttgt ctagagaatc caatttttca atatacttat
    19981 ctagattttc tttatagaat tttttattat caggatcttt ttccgctaac tgtttttcaa
    20041 tatttttagc gtaaataata ccgttttcta aatttaacca agcatgagga tcttccttcc
    20101 ctttctcact ctgaccctct aagtaaataa catctatgcc atcacttgct gcaaaatagt
    20161 ctttgttttc ctctttgttc gcatttttaa ctaattttgt aaaccaagca tttcctccag
    20221 tttccaagtt aacaccgtta taaaaaatca aatctgcttt tgaagttttt tgaacatctt
    20281 caggcaaagg ttcatattcg tggggatctt tcccaatagg tacgatactg tgtaaatcaa
    20341 ttttatcttt tgctatattt tcggtaatat ctgctaaaat cgaattagta actactactt
    20401 ttaatttttc gttcgaagcc actttatctt tgtctttact atttattgaa catgccccga
    20461 atataaaaag agcaatcaaa atccccagac ttcccagtac tgctttccat tttctcatca
    20521 taacgatctc tccttctttc ttaaaaattt ttgttttggt gagattagaa aactaattac
    20581 aaataagaat gccgctgtta aaacaatact tgatcctgct gctatgttaa aactataacc
    20641 aataaacaat ccaagcaatg atgaacttgc tccgactgtt gcagaaataa atatcatagt
    20701 tttcaagcta tttgcatata aatatgctgt agctgctggt gtaattaaca ttgcaacaat
    20761 taatatagtt cccacacttt gcatagctgt tactgctact aaagttaata gaaccatcaa
    20821 tagataatga taaaaactga cattcatccc tattgctttt gccattaatg gatcaaaaga
    20881 agtgattaat aattctttaa aaaatagaac tactataacc aacactaaaa ccgaaacgcc
    20941 aattgtaatc cacttatcaa tatcttgtac agctaaaata tttccaaata aaatatgaaa
    21001 taaatccgtt gagctatttg caactcctat aaggataact cctagtgcta aaaaagaact
    21061 gaaagtaata ccaattgctg tatctccttt tattacacta ttgcttttta taaaagtaat
    21121 aatgatagaa gctaatagac caaatacaat cgcaccaata aaaaaattaa tacctaaaat
    21181 ataagaaatt gctactcctg gcaaaacagc atgagaaata gcatcaccca taagagacat
    21241 tcctcttaaa ataataaaac aacctactgt tccagaaaca atccctataa ctacagatgt
    21301 aattagagca ttctgtaaaa agtgaaagtc atacagacca ttaataaaat tttgaagcat
    21361 attattcccc ctctccaata aatatagtat ctccataggc tttttttaaa ttttcttcat
    21421 taaatacaga ttctacactt ccgtgagcaa ttagatttcg attcaaaaga ataatattat
    21481 caaaatactt ctttaccttg cttaaatcat gatgcacaat tagcactgtt tttccctgat
    21541 gtttcaattt ttttaacgta tccataatta ttcgttcact aactaagtct atccctacaa
    21601 atggttcatc taaaaatatg aaatccgcat tttgagcaag acatcttgct aaaagtacac
    21661 gttgaaattg tcctccagat aattctccta tttgatgatt agaatatttt cccatatcga
    21721 ccttttccaa agcctttgaa actctttgcc attctgcatt tttaattctt tgaaaaactt
    21781 tcattccggc atatgttccc atagatacac attcttttac ttttattgga aaagtaaaat
    21841 caatatcagc tttttgctct acatatgcaa tcttcaccag ttttttattg atatcttcat
    21901 tattaatgga gacctctccc tgatgaggga taatatttaa cattgcttta agcaatgtgg
    21961 attttccagc accatttgga ccaataatac ctgttaaagc accttcttgt atttttaaag
    22021 aaatctcgtt caatgctaaa aaatcgttat aacaaacatt aagctttttc acttctaaca
    22081 taactttctc cttctaaatg ataatgatta tcacgatcat atggtataca aaaattatat
    22141 tttagtcaac cctaaatttt atagtttaga tttttttctg ttgctgaaga ttagaatgac
    22201 aaaactattt gaaactacgc gtatagtaag tagctttcaa aatatataaa aaaggagcgt
    22261 tctcaaatga gaacatgctc ctaaaactac tttattggcg tatggaaaaa ttatacttga
    22321 agtggatagc gggttgaata agttgataag catcaaaaca tttataaaaa ttattcaaga
    22381 ctatgtctgt tccatataca aatatgaaaa tcagtaaact ccatttaact tttttcgtag
    22441 tgtgtctttt tatacatgtt tatcgttatt aatagtgaat aggaaaaaaa ctatttttga
    22501 tcattgacaa ttaaatacaa tttttgaata acgatccctg ctattcgagc tagaaactca
    22561 agtacgccat gacctgatta gcgacaaata cttagagatt tctttttgtt ctgggaaaac
    22621 aaaatgccat gacaatagca gcaaataatg atacttccgt aaggctcgcc gtaaactggg
    22681 gagaggtgaa attcctatgt gcttacccaa agcaattcaa aaatttcata actactattt
    22741 taggagatga ttttatagaa tccagagtct acctgaataa aaaacaaacc tgtgcatttc
    22801 taggaatcga taataatact ctagataagt ggattcgtat atataatttc ccagtaataa
    22861 gagttggtgg tgtagttcgc tttgacgtag aagatattaa agactttatg aatacattta
    22921 aaataacttg aaattttctt ctcgccgcat atactaaaat tagtgaaata tactgcctta
    22981 ctttttttaa ggagtgagga ttttgcctac aaagctttca aatgggaaat ataaaactaa
    23041 tctgagatat ccaaaaaggt ttaaagaaat aacaggaatt gcatcagaaa agtatcaaaa
    23101 aacatttcca aataggcaat tggcgattaa agccgaaaat gatatgaaga agaaaataga
    23161 aaaagttttg agagaggaga atgcaaactc attagaatta aaaggaaaaa taacgtttaa
    23221 aaaattttac gaatctaaat ggcttcctag atatgaatta ggtcaaacaa ttagatcaaa
    23281 caggcctcct agtgatataa ccattagtaa tacaaaagat atatttcggt tgcatatttt
    23341 acctatgttt ggtgaatacg caatgaatta tctaaatatc aatactgaga taatatcaga
    23401 tgaattaacc aaaaaatcga aagaatatgc aaatataaaa atcataaaag gatatgttcg
    23461 ttccatgttc gatattgcag aaatactaaa ttatatcgaa tttaatcgaa ctactaaaat
    23521 tattcaaagt atcactgcac ctaaaaaaaa tgctttagaa gaaaaaagaa tacaagaagg
    23581 taagcaagca ctatcatcaa aagaacttac taattggata gaagcagtaa atgatgattt
    23641 taataatcat ttacttactt ttcatgatta cacacttttt atgattaccc tgtatcttgg
    23701 tgatagaaaa agtgagactt acgctttgca atggaaatat attgattttg aaaaacaaac
    23761 ggtccgtctt aaacatactt tagataaata tcaaagaaaa aagtttacaa aaggtcgaaa
    23821 agataccgtc attcaagtac cagaagttgt aatgacattg ctaagtgaat ggaaatcggt
    23881 acaagctgat caattgttga aattaaaaat taagcaaacg ttagatcagt atctctttac
    23941 ttatactaaa ccatctggag aagtaaattg tcctgttcat gccgattacc taaactatcg
    24001 aatcaattca attaaaagaa ggcatcctga cttagttcat ttatctccac acaaactccg
    24061 tcatacttat gcaactatag ctagacaagg tggagcagat atgaatcaaa tttcaaatgc
    24121 tttaacacac tcagatattt ctacaacaaa gatatatgtg aacacaccag atatagttga
    24181 taaggctgtt tttgaagctt ttcagagagg attgaataag tgtgattgat atgtgttctt
    24241 aaatgtgttc ttaaaaacaa aagaagcact ttccaaataa ttgaaaagtg ccagaaacgt
    24301 tgatatataa aggatattaa cgttttgaga attgtgaagc tttacgtgct ttcttaagac
    24361 ctggtttttt acgttcgacc atacgtgcgt cacgagtaag taagccagca cgtttaagag
    24421 ctaaacggaa gtcaggatca acttctagca atgcacgagc gataccatga cggatagctc
    24481 ctgattgtcc agcgtatcct ccgccgttta cgttaacaaa tacgtcatat gcgccttttg
    24541 tttcagtaac agcgaaaggt tgattgatta cttcacgcaa gtcagcatgt ggaatgtatt
    24601 cttcaacatc ttttttattt acagtaattt tgccagttcc tggtactaga cgtacgcgag
    24661 caactgcgtt tttacgacgg cctgtgccga tatattgaac ttgtgccaat gaaattccct
    24721 cctattagat taagtttgtg atgtctaaaa cttctggttg ttgtgctgca tgtggatgtt
    24781 ctgctccacc gtatacgttc aattttgtaa attgtttgcg gcctaaagtg tttttaggaa
    24841 gcataccttt tacagaagtt tcgatcaaac gacgagaatt tttagcacgc aattcgccag
    24901 cagtgattga tttcaatcct cctgggtagt tgctgtgacg gtaataaatt ttgtcagtcg
    24961 cttttttacc tgttaatttc actttgtctg cattgatgac gatgacgaaa tcgccagtat
    25021 ccaaatgagg tgtgaaagtt ggtttatttt taccacgtag tacagatgca acaactgttg
    25081 aaagacgtcc caatggaaca tcagttgcgt ctactacata ccatttacgt tctacttcgc
    25141 ctggtttggc catatatgtt gtacgcacgt tattttcctc caatttaagt tctgatgttt
    25201 gacatacact tcgagtttcc ggggctcttt gtggggcaaa caataccatt taacatatta
    25261 cccgctaatc tagtcaatgt caacgttttt ctgcttgatt gaaaaattta tatgaaactt
    25321 tacttctttt ctttctttta tataaaaggg tcgtttcctg tcaaaatgaa aaacaaaaag
    25381 aggctgtgac aaaagtcttg tcacaacccc ttattttgaa taaacggtgt tctcctgcca
    25441 actcctcggt cacaagtcac aatatccata aaacataaga agctgttttt taaaattgtt
    25501 tcttgtgact cggagctgaa cggcaggctt acaacctctt ggtaaacggt gttcaatcag
    25561 ctgaccctcg gtattagctc aaaaaacggc aaaatatgag gaactatttt gacgtttgtt
    25621 tctctaatac tcaggtcaaa acgctgattt cacaacctct ttttcggtca agttcaacaa
    25681 cagtttttac gcgtcgatct ctggcgcttt gcccaaatga gcttggatca tccagatacg
    25741 tttttctgtg gctgttttga attcgataaa gatatcttgt gttggatcgt cgccttcttc
    25801 tccagaaact tcgattcctt tttggtaaag atctgctaga taacgatagc ctgcaacaag
    25861 tgtttccaaa cgttctggaa tagtacgatc ccagcggcca atttcatcag ggatttttgt
    25921 gttatctgca aattcacgca atgtagaata aggtgaaccg tctaatgtga tcaagcgttc
    25981 ggatacttca tctaactgag cgttcaaatc atccatgaac tcatccatca ttgggtgcaa
    26041 tgtaaggaaa cctggtcctc tcatgtacca atgtgtttgg tgaacaacaa cagacatttg
    26101 gcttagatct gcaactaatt gatttaatac ttctttggtt tgttggaatt tcatctttct
    26161 tgcctcctaa cattttctta cacttaaaat aatagtagac tttcaatgaa aagtacaaca
    26221 aaatcattta cctgagaacg attttcagtt cgctttttta tccgtaaagc caatcatttt
    26281 tcagaatcag ctaattcttc tcgcagttga gcaaattgct gataagtctg tgctaaatgc
    26341 ttttgtgtcg aaacaaataa tggatagatt ttctgatatt ttttatgatc agtttcttgt
    26401 actccaactt gctctttcaa ttctatcaat tcctgtgctt cactgatact ttggatcttt
    26461 ccgatgcttc tccagcctaa aatcacagct cctaatgcgg atgcttctgt gacagtaggg
    26521 aaaacaagcg ggtaacccag tacatcagcc atcatctgac gaaaaatcgg agattgagca
    26581 aacccgcctg tagcacggat ctctgtgagc tgattttcct ggcaaactcc ttttaaaatc
    26641 cgataaaggt tgagacagat cccttctaga atcgccttca tcatttcttt ttctgtatgt
    26701 tgccgctgta aaccgataaa actgccaaac gcatctgcgt cccaaagcgg tgctcgctca
    26761 ccaagtaaat agggatgaaa aatcagacca ttggcacccg ctggcacaaa agcgatagcc
    26821 gaaagcagtg catcatatgg attttctcct ttttctttag cagattgaac gacttcctgt
    26881 aaaaatgtat gccctgccca atcaaagatg ccagcaccat tgctagtcgc tcctcccact
    26941 acccaatgtt tctgatccag aacataacaa aacgtctcca tttctggatg gaactgaggc
    27001 tgagtagtaa taaaacgaag tgctccgctc gttcctacgg tcaaagcggc gatcgtatca
    27061 ccaaaagcac ctaaccctag attagaaagc ggcccgtctg cccctcccaa aacaaatgat
    27121 atatcatttg gcaaacctag ttctgctgct gtttttgatg gtagacctgt tacttgatag
    27181 gtggaaggaa agacttgcgg caattgctct attcgaatgt tcaagtagtc aagcacttct
    27241 tctgcccatt ggaattcatg gatatcaaaa tatccagttc ctgaagctgt tccgtagtcg
    27301 ctgatcagtt cgccaaacaa tctataaaag atatagtctt tgataccgac aaaataagca
    27361 gtttttttga ataattcagg ttggctgttt tgcagccact tgattttgtt caatggtgtc
    27421 attggatgaa tcggcgtccc agtctgccga taaaattctt tgccaagcac ggtatatttc
    27481 agtttttctg cttcgtccgc tgctcggtta tctgcccaag tgatcattcg agataacggt
    27541 gttttctctt catccaacaa tatcaggcca tgcatcgcag aggagaagga gacaaattga
    27601 atgattgtgt ctgaaggcaa ctgtttaatt acttctttta taacggtgca gacaccttga
    27661 aagatcaatt cctgatcttg ttctgccatc ccttgtgttt cttggatcaa agggtagcga
    27721 aaataagcgg atgctgcttc ttgggcttca tctgtgaaag caactgcttt ggtttgtgtg
    27781 gttccgatat ctaatccgat tgaaacgttc attttttcac ctcaaatttt atgtatgtac
    27841 aaaaatttag tttaagcttg cgtatcttgg ttttgaaaag ggggagatgg cgtgttactt
    27901 tattttatca ttggttgctg tatcggatca tttttatgtt taaccgccca gcggattccg
    27961 ttaggtcact ctattattta tcctagatct cattgtgtca aatgctgtcg ttcgctttct
    28021 tggtatgaat tgattccgat tctctcgatc atcgtccaac gatttcgctg ccgctattgc
    28081 aggtgtcggt tgcccttcta ctatttgttg gcagaagcct tatgtggtgg tctttttgcc
    28141 tggttcttta ctttttcttc cgcaagaaat ttttcgacgt tcatatggat gctttcggct
    28201 ttgctatttt cgttgatgga tttattttac ttcatggtcc attctcacac gctattctgt
    28261 tcttgggcaa ttttatggat tttttggttg caggccggcg tcttccaatg gcaaagtgtg
    28321 ctgttaacgt ttatcgtagg cggtgtgtgc cttcgctatg gtcaatccta tctcggagct
    28381 ggcgatactc tgttgatgct ttcttggagt ggtggcttgt ctctagaaga gttgcttcag
    28441 atcttgtttc ttgctagtct gtttggaata tccgtttttt ctgtttactt tatctgtcat
    28501 aaaaaaaagt tggaaaagct gcctttcatt ccttttttga gcagtgcgtt gctgattgtc
    28561 cttcatctta ggtattgaaa aaaggttgtg acaaacgact tgtcacaacc tctgatatat
    28621 agtgttctct taccaaatcc ttgtgacttg gagctaggcg acaggttcac aacctctcca
    28681 ctaaatggtg ttcaatcagc tgaccctcag tattagctca aaatgctggt tttacaacct
    28741 ctgatatacg gtgttccata agtagctttt tcggcttctt atttcttgac gccccaatgt
    28801 cgacgaagga aacctgcacg tccagaacct tctcgataaa gattgtaatg gagcttgttt
    28861 ttcttgtaat aatcctgatg gtaatcttct gcaggataaa attcgcttct tggttcgatt
    28921 tttgtcacga ttggcttatc gaatcgcccg ctgtcttgca atgcttgttt tgaacgctct
    28981 gcaatatttc gttcttcttc atcattatag tagatcactg gacgataaga atcaccgcga
    29041 tcagcgaact gtcctaatgc gtcagtcgga tcggtttgct gccaatagat ttccaccaac
    29101 tgatcataag aaataacttc tggatcatat tcgatctcta ctgcttctgt atgccctgtt
    29161 gtgcctgttg ttacttgttc ataagttgga tttggtacat gcccgccagt ataaccggat
    29221 gtcacagaaa tgatccctgg ctgctcttca aatggtttga ccatacacca aaagcatccg
    29281 cctgcgaaaa ctgctttttt tgtcatataa aatccccctt ttttctcatc ataccttgat
    29341 ttccaaaagg aggataacgt tttgcttgtg acagaaaaaa cggaaactat tatatacgtg
    29401 atacggcttc tgataaatgc caaaacgaac gtacttgatc aaaaacggtt aaatcaggaa
    29461 tcacatattc agcagaagga agatttatgt tttcacctat tcccaataca tgcatgttac
    29521 cagccaaagc tgcttgacaa ccagcttctg aatcttcaat gaccaagcat gcatttggcg
    29581 gtacattcaa ttgctgtgct cctttgagaa aaacttctgg atcaggtttt gcttttgata
    29641 cttgcgtgcc gtcaataagg acatcaaaat aacttgtcaa attcgttttt tctaaaatga
    29701 ggcgtgcatt tttgcttgct gatcccaatc cgatcttgat gtttgttttt ttcaagtact
    29761 caagaacagc tattgcacca ggcaaaacgg atgtttcatc cattttttga atcgcttgaa
    29821 gatatagatt gtttttttgc tcagcaaagg ctgatttctc tttttctgag aatttctgtt
    29881 ccatttttcc aaatgacagc aatcgctcta gtgactcggt tctggatatt cctttcagtt
    29941 gctcgttttc tttctcagtg aatggaatgg acagctggtt agctaaaacc agccacgctt
    30001 cgtaatgata acgagctgtg tctacaagaa cgccatctaa atcgaatagt gctgctttga
    30061 ttttcatcga ttacctcctt gaaaatgaac gacttgaagt aaagatgcga tgtttttagc
    30121 tgtgcgatga agattatcag tggcttgctg taaagcttct tgtaaaggca cagctgcagg
    30181 aaggatcgga aaaacggccg aaatgccaac atctgctgct ttttcgcttc ctttaccaac
    30241 acttccgcaa atagctatca caggaacgcc ctctggtact cttctagcaa tcccaactgg
    30301 cgcttttcct tttagacttt gtccatctag acgtccttcg cctacgatta cgaggtctgc
    30361 ttgctgtacc cgtttatcga actccagtaa atccaatacg tagtcgatgc cagagactat
    30421 tttccctgaa gcaaatgcta acagtcctgc acccattcca ccaccagcac tactgcccga
    30481 tgcctcgatt acttgagggg caaatctcat ataaaaatca tgcatggctt ggtcagccac
    30541 tgctaattca ttttctttca agcctttttg tggtccgaaa acaaaagaag cgccttcttt
    30601 tccgcataaa agattagtaa catcagtaat gacagtgatt ttggctttaa tcagctcaat
    30661 aggcacgttc gttttatcaa ctgtagcaat tttacctaga ttttctccca gtgcttctac
    30721 tttttgatta tcttgatcgt aaaaagcata acctagacct tctgccatgc cgatgccgcc
    30781 atcgttggta ctacttccgc caacgccgat gatgatgtgt cgaacacctt gttgcaacaa
    30841 ttgtcgtatc agttctccta ctcctcgcgt ggataaccta agtggctgtt gttggtttag
    30901 tgggacatct ttcaatccga cgatttctgc catttcaaaa atcgcagttt gaccgtctga
    30961 agcgtactgg atttctttgt ttttgccaaa tgcattagta acgactgctg tttgccgctt
    31021 caaccttaaa ctgtctgcta atgatgaaac cgttccttct cctccatcgc ctataggtag
    31081 cagatcatat tcagcctgaa caaaaacttc ttgaaaacct gattgaatgg cttgtgctgc
    31141 ctctaaggct gtcagacttt ctttgaaaga atctggcgca atcagaatac gcattatttt
    31201 cccctccgtt ccaagatcag ttctttttcc agctggtatt ggtctccatt gacaaaaata
    31261 ggtaatgttt ctttcgtctc tttttttatt actatttgtt tttttgtcaa aacaaatagc
    31321 aacctgtgat ttttccaaat aaaaggaaaa gaaatttttt cccatgctga aggaagtctt
    31381 ggagaaatag aaagagttcc attcgttact gtaatacctg aaaaaccaaa tataactgct
    31441 agccatagtg ctcctaatga agcagcatga attccgtcat ctgatgaatg cggatctggt
    31501 cctaaatcaa tcaggcacgc ttcttggaat aaatgatacg cccattccat ttcttggata
    31561 cggcaagcct ctatggcgtg aatcgcttta ctcaacgatg agtcgtggat tgttttgttt
    31621 tcatagtaat gcaaattctt agaaacgatt ggttgagaaa aatactcagg taataagtat
    31681 aacagcatga caagatctgc ttgcttgctg acttggagtt catttacttc gctacgggca
    31741 taatcaagta aaatagactg gctgccagga cgttctttgt aagtggtcaa atcaataact
    31801 ggcttactga gatacgtgtc atcttgcgga atcagctgtt ctttgttagg cactggcaaa
    31861 tataacttat ctaaaaaatt ttgacattct ttcaaaaaaa gcggatcgcc agcttgatat
    31921 ttaacagcta aggccacatt ataatgcgcc agatagttcg tatatgcatt attgtcgata
    31981 tattctgtgt attcatctgg accaatgact ctgtgtatct ctaaatgatc atcgactttt
    32041 acagctctgc tcagccaaaa agctgctgtt tcatgaatga gcgtttttcc ctttgtttct
    32101 aaaaacgatt gatcctctgt tgcttggaca tactcttgaa cggcataagc aaggtctgca
    32161 ctgatatgat gttctgcggc agctgaagca acttcttgtc ttgttccggt tttgatattc
    32221 aatgcagcaa atttgggtgt ctcttcctga ccagtgtgtg cgctttccca tgggaaaagt
    32281 gcaccttgat agtgataatc tcttgctttt tgccgtgcgc cttctaatcg atcataacgg
    32341 tacagcagca aattctttgc tacttcagga aactgatata aaaagaaagg caggataaaa
    32401 atttccgtat cccagaaaac atgcccttta taaccttcgc cagttagtcc cttcgcccct
    32461 acactaacac ttggatcatt gcggggagtc ataatctgta aatggtaagc agcgaagtcc
    32521 atcgctaact gatcgaaaga agaatcgcct tggatcgtta tccgatgctg atgccaaaaa
    32581 tctgtccaat aagacatcga ctgctctaaa atttctgtat aactaacgct tttctgggct
    32641 tgggcaatac ttttcttaaa cgggtctgtt tgctgaagag aggtagagag cacgatgatc
    32701 ttctccagcg taaaggacgt attttttctg atcttttctg aaaaggaagc ggtgatccgg
    32761 cgattttttg cttggaatac acctgcttta ttgaatttag ctgcaatccc aatatcaatt
    32821 ttactttctg ttgtctggta ggatgcactg atcaaatcgt caccaaatac tcgcagttct
    32881 ttttcaacca actgctgtgt accaaaattg gtttgctgtc catcgatacc cgttttcaca
    32941 tctacttggc agtcttcggt caacggcttg atgaccactt tcagggctgc ctgatggaga
    33001 ttattttgat cagcaacacg acaagtaatg acttccactt tttttccttg agcagtttca
    33061 atgagcgcat agcgtttaag ctctcccatt tctaaatcta gtgcccgctc gtatgcaaga
    33121 actttcatcg tctcaagaga aaaatgttca ccgtcgattt ttatttccca tctgatgaca
    33181 tctgggagat tcactaactc ggctgattct gctcctaaag gtcggttata tacaccagct
    33241 gcaaacattc cccttacttg attccgatag tcttcctcct ggcttcctct gataccaaaa
    33301 aatccatttc caatggtcat ctgactcgca tacttattga tatactctaa atcaaactgt
    33361 ttttcttcta ctttcataag gtaataactt ctcctgtttc tgccgaacga tataaatttt
    33421 ccaccagatt ctggatgaca tatccttgcc agccatctgc aatcatctga gtctggtcac
    33481 cgagcgcccg attcacaaaa tgagccatac ttagttcatg tgggtcatta actggcgtgg
    33541 tcttttgtaa ggaaaccagc tttccatcta catcttcgaa tagttcgcct ggatacagat
    33601 tagcacctgc ctgatcgcca caaaaagcca cattcagttt gtcttctctg atattcaaag
    33661 caaacgaagt attcagttgg atgatcttgc cgttttctaa ttccagtgtg ccaaaaattg
    33721 catcttctac tgtgaatttg cttggatccc atttcccgaa tttccctgaa ttttttttcg
    33781 taccaatttt ttggaagctg tacgcccgaa cgctttttac tttaggaaat ccagtaatat
    33841 acatcgctgt atcaagcatg tgaatgccat aatcgatcaa aggcccgcct ccttgaagtt
    33901 ctttgtcgat aaacgttccc cagcctggaa caccagaccg acgaagcgca ttcgcttcta
    33961 cgtaataaat atccccgaga acatcaagat gattcttgat cattgttgct tcgtcagaaa
    34021 aacgatgctg gaatccataa gccaaaatct gcccgttttt atcggctgtc tctgccatct
    34081 ttcttgcttg atcagctgtc atagctggtg gtttctcgca catcactgaa gcattagctt
    34141 cgagggcagc caatacatgt tcgaagtgaa agcgattggg tgtacagaca ctcacgatat
    34201 cgggagaaat ctcattcagc atttgtgtaa ctgatgtata cgcatgagaa aatcctgtac
    34261 gtcggcaaaa gtcttctgct cgctggctga ctggatcaac cacagcggcc aattctaaga
    34321 catctgatct tttttgataa taagcagcat gtactttttc tgctacttgt ccagcgccta
    34381 tgattgctac tttcttcatt tatttctctc tcctttgctt atttacttat tgttattctt
    34441 tctctattct ttctctaaat catttaaatg acgcgctgtt tccagacagt ttttcaagta
    34501 ttgcaatgat tcctgataaa ttttcacttc attttctcct cgcaaacgac attcatatac
    34561 catataacct tcatactcat tttgaagtag cgtttgaaaa tggcgagtaa aatcaattgc
    34621 gccagatcct ggttggtagc gatgattatc tgctaaatgg atatgaccca catactcttt
    34681 ttgcgtagtc aatgcttttg aaatatcatc ttcttcaata ttcatatgat aaaaatcagc
    34741 gatgatctgc acatgtttca attgattaga gtcaatgtaa tgacgcgcat ccgctaacaa
    34801 attgatcata tggtcttggt atcggttcaa aggctccaaa taaataaccg tacctgtttc
    34861 ttcggctact ttatctaaat aacgaagtga ttcgctgact gctttatagt ctccttcttt
    34921 gcttcggggt gaaaccatcg gcggtaatcg ataagtgaac attccccaag ctgctgggat
    34981 aacgattcct ttgcctccaa cctcgcttag tgcacgcaaa atctcagcaa tttgacgtac
    35041 accattaaga cgccgttctt cgataaagtc accgatccac ccatcgtagc cgttacaagc
    35101 tgtcactact gggatccctg tttctttgat tgcctgtttg actgtgtcga tattttctat
    35161 taacaactta ccgtcgattt cgtattcttc aaatccacag tctttgataa atctaaattt
    35221 ttctaaaatg tctcttggga aaaatgcttg atcttgcgta ccgaatttca ttgttctcct
    35281 cctcggactt gaacgcccat tttgatactt tcttctgggt gctggtctac ataacgcatg
    35341 taagcttctg cgctttctgc aaatggcaca actggatcaa tgatttcttc acaatccaag
    35401 taaccgttca tcaataattc ccaacatgtg tcctcgattc gtttacggtt ccatcttggg
    35461 taatctgggt ttggttcact agatgctcga gcaaagacga tttttgcatt attgaaatgt
    35521 gcttcgcgac caagattaaa cccagctgga aaaggtttag caaaggcaac ataagaaatg
    35581 atcccgccat agccgatccc tattaaagaa gcttgtaatg cggcagcact gccactcgtt
    35641 tcaataactg aatctactcc acgtttatct gtcaattcct ttagacgaaa accgacatct
    35701 gctttcatag gatctagcgt gtaatcggca ccgtgttttt ctgctaattc tctacgctgc
    35761 gctatgggat cgatcccaac gatggtagta gcacctgctt ttttagctaa ttgaatcgca
    35821 atttgcccga tagcgcctaa accgaagatt gctacagcat cacctgctcg aacatgtgct
    35881 tcccggatac cacctaaagc gaattgagca ggatcgtaac atactgcatt tttccattga
    35941 tccaaccgat caagtttacg tagtttataa ttatcaacac cgtttgtaat cagcgtttca
    36001 cgaatgggtc cataagtgca gactaactct cctttttgat actctgtcac caaactgccc
    36061 gtttcttcga tcgtaccgac aatcatattt cctaattgaa attcgccaaa taccacaccg
    36121 cgttggcttc cttcttctct tggcaagaat aggcgccatt cttctgaaaa ttcttcatcg
    36181 ataaatggac ttaatcctct aaagtcgaca atttctgttc ctggttttgg agtagcgaaa
    36241 attactcgga tacgtacttc gttcttttcg atctcacggt cttcgtaatc tttgaatccg
    36301 gctattcttg gttctaatgc aactaatttt ttcatctttt ctttcctctt tcttttcttt
    36361 ttatcctttt acgccgcctt cgactaaatc acctttaata aatcgttcag ataacgcata
    36421 cataatgatg actggtaaag cggtcaccaa tgaagcggcc atcattcgtc cccaaatgta
    36481 gtctggtgta ctgaataatg tgttcaaacc gattggtaaa gtaaattttt cggaggtcga
    36541 caggaaaatc gaagcaaata aataatcatt ccacgaaacc ataaagcaat aaacgaagac
    36601 agaaatgatt ccagaaatag ccaatggtat aatgatccga ataatgatct ggatccggct
    36661 aagaccatcg atcattgctg cttcttcaat atcagtcgga atcgtgtcaa aataactttt
    36721 tagcatgaat atcgcagtag gaagtgtttg aacgaccatc gtgataatca gtgctgtatt
    36781 ggtatcatac aaacctagac cggaaatgat tttaaataat ggaacaacga tcaaaatacc
    36841 ggaaaacata taaacggtgt aaaagctagc attgattttc gagcgagctt tgaagcgtag
    36901 ttttgacaaa gcgtatgccc cgctgatccc aatcactact gcagttacgg aggctatcaa
    36961 ggagatcact aaactatttt tgaaatacat gatgaatgga aaaatctcag cattgaaaat
    37021 atcctgataa tgttcaaaag tccattcttt cggaaaaata gtttgtgtcg ttgcaattgc
    37081 ttcttgtgaa tccttgaatg atgtcatcag catgatcaaa aatggaaaca aattgacagc
    37141 taatagcaac aaaatcgtcg cgtaaaataa gattttcctg ctctttttat ttttcatttc
    37201 aagtccacct tctttcgtga gagcaagacg accgctgcaa taatgatgaa caatagaatc
    37261 gatatagctg ctgcttttcc aagatcattg aacgcaaagg ctgtctgata caaataaacg
    37321 cctaagatat tgactttatt cgtcagtaga tagacatctg taaacatata gaacatccag
    37381 atagatcgta aaacaatcac agttgctagt actggtttga ttgccggcag tgtcacgatc
    37441 ttgaacttct gccaagcatt tgcaccgtcc atttcagctg cttcataaag cgttttgtcg
    37501 actgtttgca aaatagccaa aaatgaaata aacgcatacg gatagtatct ccagatagca
    37561 aacaaaacga ccataaagaa gctagcaact ggctggtcga accacagcgg tgcttcgttg
    37621 aaaagctgca acctatctac taacagatca ttgacgattc cataactatt attgaacata
    37681 tatttccaag caaaaatcaa agaaatagat ggcgtaacat aacttaataa aatcagtgaa
    37741 cgcaccgttt tcctaaatct gaacttgcgg ttaagcaaaa tagctacggc taatcctaaa
    37801 gctgtactgc ctgctacaac taagaatgtg taagcaactg ttaaacctaa agaacgataa
    37861 aattcactat cacttaaaat ttcaagataa ttgcttaatc cgataaaaac ggcgtctgag
    37921 ttaggattca gcggcatatc gaaaaaactg atctgaatat tggaaagcat tggaatcagc
    37981 actaaaacag ctaataaaag catgcttggt gctaaaagtc ccatggctaa ttttaagtca
    38041 gaatgtttcc ttggacgatt cattgaaacc tccccctgat aatctgccaa tagctatcta
    38101 cttttttcga acaccattgg cagaacttct tgttatttcg tatttttttg tgcgtcagct
    38161 aaagcttgat cgacatcttc gtttccgaca gttacttggt tcactgcttt tccgatcaca
    38221 cctgagcttg tgatatctcc cattttcgtg aaattctttt ccccgactag tccaaagact
    38281 tgtacttcat caaaagcact agcaatctca gatggcagct cgccaaaaga cttgatgact
    38341 tcattttctt tgtaaccatc cagttcgact acttgtttat tcactggttg agcaccacca
    38401 ggagacatca agacccactt ttccatgttt ttcggttgag ataagaaagc tgtgaatgct
    38461 tcagcagcct ctttttgttt ctcgtctaga ccagaagaaa tcgtcaatcc agatactgtg
    38521 ccatagacag cttgatcttt ttgtgttggg atagcaaaac cgatattttc tgagttcccc
    38581 gcttcataaa cagaaggcaa aatgtaagtt gaatagattg ccattggtgc tgttccattc
    38641 atgaaagcat ctttgatttc tgtcacatca ttagagcctg gcatcgtgta ttgggataag
    38701 ttttgataaa acgacaatgc ttctctcatc ttttccgtat cgatcgttac ctcgccttca
    38761 tcatccaaaa catttgctcc atttgacaaa gcaaattgag agaacgcttg ctcactcatt
    38821 gttccttcag cagtaggcat agcaattcca tattgtttat tctctttatc tgtgaaatgt
    38881 tgagcaattt ttaaaatatc atcccaattt tctggttctg aaaaaccagc ttctgataat
    38941 ttttctttgt cataccaaat accttgaacc cagccgctaa ttggtgcagc aatatagctt
    39001 tttccatctt ctgaacgaac aaggtttttg gctccgtcat aataattatc ttctccagct
    39061 tcctcgataa cgttttggac tgccgtctga tcaatcagtt catctttatc catcacttta
    39121 gcaaaatcct gactgacttc cattacttct ggtaatttac cggaacgagc taacgtaacg
    39181 atttttgtat tgaacgctga ttcttctacc ggcacttgct tgattttgat gtctggattt
    39241 tctttttcaa aatcagcaac cagttcattg atcacactta atcgttcctg ttcgacagaa
    39301 gagtgcatga attgaatcgt tgtctgttgt gattcgttag agctttgact ttcaccacaa
    39361 gcagtaagaa gaaatccagt agctagcaga caggtagtaa caataatttt tttcattttt
    39421 ttccacctct tattttatta ttttcagcca catatattca tacggctgga atatcaactt
    39481 tcgttcttga ctgcaatgga taatttgtcc agaaaacagg tctaggtaat ctccttttgg
    39541 taaaagataa cctgctgatt gattggaaaa attatggata actaagactt tctcttcctt
    39601 cgcttcgcga ataaaactga taaaagaatc tgctgagtcc agtaagtaaa tcaaactatt
    39661 cggatggaag gctttttctt tttttcgaat agatagcatc tgggtcaatt caccatatac
    39721 tgctgttctt aatggatctg tctgcaactc tttctcaata tctgctaaca aaaatttttg
    39781 ccgattgatt tttcgattct ctcctgtttt ttctacgcct ttgtaatcat ttcgacttcc
    39841 cagcacactt tgaatataaa ttgccggcac gcctggtaac gttaccaaaa atgaatgagc
    39901 agctaaaaag cgtttgcttc tccattccgt cgtatctgtt ggagaactta acgcatctag
    39961 atacgtagcg tttatctcat atggactggt cgttccatct gggtttttct tgtagtttac
    40021 tcgggcaccc tctttcttca gctgttccac taattcaagg atcgtttttt ccggcagaat
    40081 acctcgtacc ggattcaatc cgataccgtc atgggaggct aaaaaattaa aaaaagttgt
    40141 ttttttcgat gcgaaatcga tggtttttaa ccattctttc aacttacttg tattacccga
    40201 acgaatcgct tgaagggtca aaggtggtag cgaaaactga tagaccatct gtgcttcatt
    40261 ctcgccattc ccaaaataac tgatattatc tttgtgtggc acatttgtct cagtgatcaa
    40321 taccgttcct ggagctgctt catcagcgat tgcacggaat aattggatga ttttatgcgt
    40381 ttctggcagg tgtatacaag aagttcctag ttctttccat aaaaatccca cagcgtccaa
    40441 gcgaataaat gaagcgcctt tttctaagta aaacaaaaga atatcgatca ttttcaataa
    40501 aacttctgga tttttaaaat tcaagtctac ttgatcttcg ctaaaagtcg tccatacgtg
    40561 tttttctttc ccgtttttgt caatgaacgt tgtaagtaac gggctggtcc ttggtctagt
    40621 cacgctgctt gtatctgtat ccagtggcaa atcaataaaa aaatctttgt acgtatcatt
    40681 caagtttaaa tattcctgaa accaagtact ttcggcagac atatgattgc agacaaagtc
    40741 gaacattaac cgtgtttcgc ttctcatttc ttctatatct tcccatgtgc ccaaacggtc
    40801 gttgattgcc tgataattta caacggagaa accatcatca gatgtatatg ggaaaaatgg
    40861 caagaaatgt acaactctaa atgtatctgc caaatacttt tgataaaatt tcttaaatgt
    40921 acgcaaagtt ggttgctgct cttcaaaaaa ttgatcccca taagtaatca agaaaatatc
    40981 ttcttcgtcc caatgcagtt tgcctgcctg gcctttcttc tgctgataaa cactgacttt
    41041 ttgttcaatc atagaggcgg ttttctcaga caaatctgag tcgcgataaa tcgcttgtaa
    41101 aagttttgta tacatcggat tcccctttct tttttgggag cgttcccaac gatttgatca
    41161 tattatggaa aacgcttact gtcaatagca tttttttaaa taattttgat atacacaaaa
    41221 atacaaataa gaaagcttat gtatcaataa ttaatagctt cttaaaaaaa tatggtctct
    41281 catgatcatg caagctattc ctaaattttc aaaatcatag acaaaaaaga atgtctattg
    41341 tactatggaa ttattctaaa acgctcccaa aaaggaggtg catataaaat tgaaacctac
    41401 tatttatgac attgcgaaaa aagctaatgt ctcaaaatca actgtctcca gagtattaaa
    41461 taaccagcca aatatctctg ataaagcaaa agcaagtgta cttcaagcaa tcaaagaatt
    41521 gaattatgaa ccgagcaaaa ttgcaagagg actgtcagga aatggttttg acgcgattct
    41581 agcattatct catcgtactt ctcatacgac ctatggcaat cctttttttt cagatgttat
    41641 tcaatccatt tctgccgttt ctgaacaaaa taacttcgac ttgattctac aaacggcaaa
    41701 aacaccagaa gaagagctag aaaaatgcgc gaaaaagatc caagaaaaaa tgatccgagg
    41761 aattattatt ttaagctcta cgatggatga aacacttctt tttgaattgg atcaatataa
    41821 tattcctatt gtggtaatcg gaaaagtcgg aaacgactac acgaacatct actcagtgga
    41881 cacggataat ttccaggata gttacgattt ggtggaaaca ctgatcaaga atggacaccg
    41941 tcatatcggc tgtctccatg ctccgacaga agtttatgta tccaatgatc gtctcaatgg
    42001 ttaccgtcaa tgcttatttg atcaccacct agatatccgt aatgaatgga ttatagacgg
    42061 cggctttcgc ttagaagata gcatggcagc aatgaatgat ttaatgaagc tacgagaaag
    42121 accaactgcg attttttcta ccgatgcatt aaaatcattg agtatttaca aagtggcaga
    42181 acaatctgga aagcagatac ctgatgatct ttcgatcgca ggattcagtg atcccttatt
    42241 gtcctcccta ttttcgccac agttgactcg gatagacatt ccgacaaatc agttaggaat
    42301 caaagccaca gaacttttat tccaactaat ccaccaccaa taccctgaag aaaaaagaat
    42361 cattctttca accgagatta ctctcacaga ttcgatccaa caacaagaaa tgtagaacag
    42421 aattataaaa gaacagaaac actgaggata agtcagttac tctgttcttt tttcaatcac
    42481 tcatccctcg ttaccccctc ccggtatttt agcgaatttt tcttattttc catctgtttt
    42541 agatagctgt ggataactat aatgtttgtg ttaaactatt ttttccacag ggaagaaaaa
    42601 actattttta accacgttgt gtataagttt ttatagttcg tttttttaaa cacatttacc
    42661 cttatttcaa tccatgttat ttggggatac ttatctctaa aacataaata gaataacaga
    42721 catgtctact ggttttgttt tattcgagga attattcaat aatagctagc aaaatattaa
    42781 aaataaccgc tctatcttcc catcaattta ttttgcattc aaaaaatgat ctatctttac
    42841 ttttgttgat tgacttctct tggaagatga tcgttccgat accttaattg gatacgaacc
    42901 gatgttgtta agatcctttc ccacttttct tctagttaga caaattattt tagacaaacc
    42961 aaaaaagagg ttgtgacaat aatttgtcac aacctctttt ttaaaatatc tattttgttt
    43021 gattgttcat tgcgtcttct gcagccattt gcgcttcaat atctgaaata ctgtagacat
    43081 tttcgcttgc tacaccgact tcttttggtt ccatgtcacg ataacgttgc ataccagtac
    43141 cagctgggat gatcttacct ataataacat tttctttcaa accaagaaga ggatctttct
    43201 taccgcggat cgcagcatcc gtcaagacac gagttgtttc ttggaatgat gcagcagata
    43261 agaaactatt tgtttctaat gatgcttttg tgatacctag taagacaggt cgagaagttg
    43321 caggaacgcc tcctgagact agcgtattgt agttttgatc tttaaagtct gcgatgtcta
    43381 ataatgtacc tggtaagata tctgtatcac ctggatccat tacacgaact ttacgcagca
    43441 tttgacgaac cattacttcg atatgcttgt cgccgatttc taccccttgc atacggtaaa
    43501 cacgttgtac ttcacgaagc aagtagtttt caacagataa tacatcgcga acttgcaaca
    43561 attgttttgg atcgattgat ccttctgtca atggtgcacc acggtggatc atatcgcctt
    43621 ctgctacttt catacgtgct gtataaggaa cagtatacgt acgtgtatct gtttttcctt
    43681 tgattgttac ttctttggta cgtgttgcag gatcttctga gatatcgatg acatcccctg
    43741 ttacttctgt gatcactgct tgccctttcg gattacgtgc ttcaaagatt tcttggacac
    43801 gaggaagacc ttgagtgata tcgtctccgg caaccccacc tgtatggaat gtacgcatcg
    43861 tcaactgagt accaggttca ccgattgatt gagcggcgat cgtaccaact gcttctccga
    43921 cttcaacgtc agagccagtt gcaaggttgc gtccgtaaca atgtttgcag acaccatgtt
    43981 ttgtattaca tgtgaagaca gaacggattg tcactgtttc gacacctgca tcaacgattt
    44041 gttttgcgat gtcttcggaa atcaattggt ttcctgcaat gatcaattca tttgtttcag
    44101 gatgacgtac ttctttacgg gtgtaacgtc ccactaaacg atcttctaat gattcgatga
    44161 tttcatttcc ctcgcggatg gcttggattt caagacctcg gtcagtaccg caatcttctt
    44221 cacggatgat tacatcttgg gccacgtcaa ccagacgacg agtcaagtaa cctgaatcgg
    44281 cagtcttcaa ggctgtatcg gtcatccctt tacgagcccc gtgagttgag ataaacattt
    44341 ccaagacgga tagtccttca cggaagttcg aaatgattgg caattccatg attcgaccat
    44401 taggagcggc catcaaacca cgcattccgg caagctgtgt aaagttggaa atgttaccac
    44461 gagctccaga atcagacatc atgaagattg gatttttcgc ttccaaacct tccatcagtt
    44521 tttgttggat gctgtctttt gcagcattcc aaacagcaat gacacgttca taacgttcgt
    44581 catctgtgat cagaccacga cggaattgtt ttgtaatagt ttcaacttgt ttatgggcat
    44641 tttcaatgat ttcttgtttt tcattcagta cactgatgtc agcgatccct actgtgatac
    44701 cggcatgtgt cgaatgtttg taccctaagt ctttcatacg gtcaagcatc ttagaagttt
    44761 ctgttacttt gaatcgtttg aagacttctg cgatgatgtt tcctaagttt ttcttcttga
    44821 acggtaatac taattcttgc tctttgatat gtgcaggaat atcagtacca gcttcaacaa
    44881 agtatttgtc cggtgtttgg acggtcaagt tgaagtctgt tggttcattc agataaggga
    44941 attcaggagg catgatctcg ttgaagatga tcttaccaac tgttgtgatc atcgtacgtt
    45001 ctttttgcca ttcagtaaat ggtttttcac ctaatgagtt agggttcaca ccgatacgtg
    45061 aatgtaaatg aacatatccg ttgcgccatg cgataacggc ttcgttttga tctctgaaga
    45121 ccattccttc gccttcgcgt ccttcttctt ccattgtcaa gtagtagtta cctagaacca
    45181 tatcttgaga aggagtaaca actggtttac catctttagg gttcaagatg ttttgagcag
    45241 ctaacatcaa catacgtgct tctgcttgtg cttcttcatt caatggaacg tggacagcca
    45301 tttggtctcc atcgaaatcg gcattataag cttcacatac aagtggatgc aaacggattg
    45361 cgcgtccttg tactaaaact ggttcgaatg cttggatacc aagtcggtga agcgtaggtg
    45421 cccggttcag caatactggg tgttccttga ttacgtcttc taaaacatcc catacttcgt
    45481 cttcttgacg ttcgatttta cgtttcgcat ttttgatgtt tgaagctagt tcgcgttgaa
    45541 caagctcacg catcacgaat ggtttgaata attcaatggc catttctttt ggaagtccac
    45601 attggtacat cttcaagaaa ggtccaacaa cgataacgga acgacctgag tagtcgacac
    45661 gtttacctag caagttttga cggaaacgac cttgtttccc tttcaacata tgagaaagag
    45721 atttcaatgg gcggttacct ggtccagtaa ctggacgtcc gcgacgaccg ttatcgatca
    45781 atgcatctac tgcttcttgc agcatacgtt tttcattttg aacgatgatt ccaggtgcat
    45841 tcaagtctaa caaacgtttc aaacggttat tacggttgat tacacgacgg taaagatcat
    45901 tcaagtcact tgttgcaaaa cgtccacctt ctaattgaac cattgggcgt aaatctggtg
    45961 gaataacagg gataacgtcc ataaccatcc agcttggttg atttcctgaa gcgcggaatg
    46021 cttctaaaat atccaaacgg cggattgcac gtgtacgttt ttgtccttgt gcagatttca
    46081 attcttcttt caattcggct gcttctgcat ctagatcaac attatcaagt aattgtttaa
    46141 tcgcttctgc acccatagca gcttggaatt cttgtccata ttgttcacgt ttttcgcgat
    46201 attcgcgttc tgtcaacaat tgttttttct ctaaattcgt atttcctggt tcgatgacta
    46261 catatgaagc aaaataaatg atttcttcca aagcgcgtgg gctcatgtct aatacaagac
    46321 ccatacgtga aggaatccct ttaaagtacc agatatgtga aacaggagct gctaattcaa
    46381 tatgtcccat gcgttcacgg cgaactttcg aacgagtaac ttccactccg catcgatcac
    46441 aaacgattcc tttataacgg atacgtttgt attttccgca ggcacattcc cagtcttttg
    46501 taggaccaaa aatccgctca cagaacaacc cttcacgttc aggttttaag gtacgatagt
    46561 taatcgtctc aggttttttt acttcaccgt aagaccagct tctgattttt tcgggagaag
    46621 ccaaacctat ttgcatactt tcgaatttat ttacatcgat caaaaggggt tccctccatt
    46681 tcatttaatg gcaaaccgca agacggctga tcttcgaccg ccttgcagct catagaccgg
    46741 attagtctaa attatcttct gcggtttcaa agttttggat gacatcgtct ttttcatctt
    46801 ccacttgttc agcagctttc ttttctaatt ctttggcagt ttgctgttcg gcaaattttg
    46861 tcaaagcatc aacagtgatc aaatcgtcgt cttcatcgtc catgtcgcga agttcgatct
    46921 ctgtttcttc gatatccaag acacgcatgt ccagacctag tgattgcaat tctttcacca
    46981 atacgcggaa tgattccggt acacctggtt ttggaattgg ttcacccttg acgattgctt
    47041 cgtatgtttt cacacgtccg actacgtcat ctgatttgta tgtcaagatt tcttgtaaag
    47101 tataagccgc accgtaagct tccagtgccc atacttccat ttcaccaaaa cgttgtccac
    47161 caaattgagc tttacctccc aatggttgtt gtgtaaccaa tgagtaaggt ccgattgaac
    47221 gtgcatgcaa tttgtcatcg accatgtgag caagtttgat catgtacatc acaccgacag
    47281 acacacgtcc atcaaatggt tcacctgtac gtccgtcgta cagaacagtt ttcgcatcgc
    47341 tggccatacc ggcttcacga actgtttccc atacgtcttc atcacttgca ccatcaaata
    47401 ctggcgttgc tacgtggata cctaactgac gtgcagccat acccaagtgt aattccaata
    47461 cttgcccgat gttcatacgt gaaggcaccc ctaaaggatt cagcatgata tcgattggtg
    47521 ttccatcagg taggaatggc atatcttctt ccggcataat acgggataca acccctttat
    47581 ttccgtgacg accggccatt ttatcccctt catggatttt acgtttttga acgatataaa
    47641 cacgaactaa catgtttacg cctggtgaca attcatctcc agcttcacga gtaaagattt
    47701 tcacatcgtg aacgataccg ccgccgccat gtggtacacg taatgatgta tcgcggactt
    47761 cacgagcttt ttcgccaaag atcgcatgca atagacgttc ttctgcagat aattctgtta
    47821 ccccttttgg cgtgactttc cctacaagca aatcgccatc ttggacttct gcaccaatac
    47881 ggatgatacc catttcgtct aaatctttta atgcgtcttc cccgacatta gggatttcgc
    47941 gtgtgatttc ttcaggtcct aattttgtat cgcgggcttc tgattcatat tcttcaatat
    48001 ggatagaagt gtatacgtca tcttttacca aacgacggct catgatgatc gcatcttcat
    48061 agttgtaccc ttcccaagtc atgaaaccaa ctaaaacgtt ttggcctaga gccatttcgc
    48121 cttgttccat agatggtccg tctgccagag tatccccttt ttcgactttc tcacccaaat
    48181 gaacgattgg acgttggttg tagcttgttc cggagtttga acgacggaat tttgttacat
    48241 cgtatttatc taatgcaccg ttgtcgcggc gtacgcggat ttcagaagca tcaacgtatt
    48301 caacgacacc gtcatgttta cataacaaag cagctcctga gtcatgcgct gatttgtatt
    48361 ccataccagt accaacccat ggtgcttgag gattgatcaa cggcactgct tgacgctgca
    48421 tgttggcacc catcaaggca cggttggagt catcgttttc caagaaagga atacatgctg
    48481 tcgcaacggc aactacttgt ttaggtgata cgtccatgta gtctacttta tcaatagata
    48541 cttctaagtt ttcactttga gcacgagcca taacaacgtc ttgagcaaat gttccatctt
    48601 cattcaatgg gctgtttgct tgggctacga tgtaatggtc ttcgatatca gctgtcaagt
    48661 agtcgatttg gtcagtaaca cgacctgttt cacggtctac acgacgataa ggtgtttcga
    48721 taaatccgaa tttatttact ttcgcataac tggacaaact attgatcaac ccgatgtttg
    48781 ggccttcagg agtttcgatc ggacacatac ggccatagtg ggagtagtga acgtcacgaa
    48841 cttcataacc ggcgcggtca cgagtcaatc cgccaggtcc taaggctgaa agacgacgtt
    48901 tgtgtgtcaa ttcgccaaga gggtttgttt gatccatgaa ctgtgacaac tgagaagaac
    48961 caaagaattc tttgatactt gccacaactg gacggatatt gatcaattgt tgaggtgtca
    49021 atgtttcagt atcttggatc gacattcttt cacgaaccac acgttccata cgtgctagcc
    49081 cgatacggaa ttggttttgt aaaagttcac cgactgaacg gatacgacga tttcctaagt
    49141 ggtcgatatc atcgacattg ccgatacctt ccatcaagtt caagaaatag ctcattgaag
    49201 caatgatgtc ggctggacga accgttttta cagctgcttc tggatagccg tttccgatca
    49261 cgtttacttc acgttcaggg tctcttggtg aaaatacttt gatgacttgg acagtcattg
    49321 gatctgtgac cacaccgtct tctgatggat agtaagtcac tgaatttagg ccattatcaa
    49381 tgaaaggagc tagtgtttcc atgacttggt gagtcagaac tgttcctttt tcaacgatga
    49441 tctcgcctgt ttctggatca actaatgttt cagctaatgt cagattcaat agacgtgttt
    49501 tcaaatctaa ttttttgttt actttgtaac gaccaacgtt tgctaagtcg taacgttttg
    49561 gatcgaagaa acgagcattc agcaagttac gtgagctgtc tgctgttttt ggttcgcctg
    49621 gacgcaaacg ttcgtaaacg tctttcaaac cttcttctgt tcttgagtcg cttgcgtttt
    49681 tatgcaagtc tttttcgact gtattgcgta atgtttcgct gtcgccgaag atttcaaaga
    49741 tcgtatcatc ggaaccaaat cctaacgcac gaactaatac ggttaaagga atcttacgtg
    49801 tacggtcgat acgtacataa gagatgtctt ttgcatcagt ttccatttct agccaagctc
    49861 cacggttagg gataacagta gaaccgaatc cttcttttcc gtttttgtcg acttttccat
    49921 ggaaatagac acctggtgaa cggactaact gagaaacgat tactcgctct gccccgttga
    49981 tgatgaatgt tccttgttct gtcattaatg ggaaatcacc gaagaatact tcttgtgctt
    50041 tgatttcgcc agtctcgcgg tttgtcaaac gcaatgttac atgtaatggt gcagagtagt
    50101 ttgcatcatg cgcacgagct tctgctactg tatactttgg ttctttcaat tcataatcta
    50161 caaattccaa agatagattg ccattgaagt catcgattgg caaaatgtct tcaaacattt
    50221 ctctaagtcc ctcatctaag aaccattggt aagagtctgt ttgaatttcg atcaaatttg
    50281 gtaattccaa cacttcactg attcttgcga aacttctacg ttcgcgatgt tttccgtatt
    50341 ttactacgtg tccagccaag ctcttcaccc ctctaaaatg tcattcaaaa agactacccg
    50401 atcaattgtt acaataatat ttcaaatatt atttttaggt aacaacagac attttttgcg
    50461 gcttttaggc aaaaaaaaac caaaaataga acattctctt gaaaccttac ttcgtgagct
    50521 tctacttttg tttccttata ttaaagccga aacggacaat atttcgtttc tgccttttct
    50581 tcatcagata gtttttgcaa cttcatatac tacacttatt ttctataaaa gtcaacactt
    50641 cctggcgttt cttctgcttt ttcgcaacat tttcttaatg ttttcaattt tatgttttca
    50701 attatctgac aattcttaat tgacagacct ttttatctcg gttatacttt agaaaattca
    50761 ttttgtggag gaacttctat gcgaaccaac atcaaccaaa tcgcaccaac acaactgaat
    50821 aacttttact ttagctactt tagataggtt tgtattcatc atgatggata caaacgttaa
    50881 acgacagttt gtatctatga tggctaaaga actttgtatt gcagcaaaaa gccacgtaga
    50941 caaaatgcta agtggctgag cagtgaaaat acaaagtaaa agtatcaagc attctacttt
    51001 ttcaatttat tttccgactt ttcaatccat ttggacattt tgtctgaatg gattttttgt
    51061 ttcccaaaat taaggaggaa aaagaaatga aaaaaaatat aatcatcgga ttaggcgctg
    51121 ccctgctact agctttagga ggttgcgcac aaaaagacaa ttccgcagag gcatcagatg
    51181 gacaaaaaac aattggcgta ttgcaacttg tacaacataa ctcgttagat gctgcctata
    51241 aaggattcaa agaaggatta gcagaagaag ggtataaaga aggcgataat ctaacaatcg
    51301 attatcaaaa tgcccaaaac aatcaagaca acctgaaaag tatgagtgag aaactcgtca
    51361 aagaagatcc tgatttgcta cttggaatcg ccactccagc agcacaatct ttattaaatg
    51421 aaacaaccac tatcccgatg actgtcacag cggtcacaga cttggaacaa gcccatttag
    51481 tcacatccaa tgaaaaacct ggcggcaatg tgactggcac gacagatatg gtgccaatcg
    51541 aaaaacaaat cggcttactg ttaaatatcg tgccagacgc caaaacaatc ggcatcatgt
    51601 ataacaacgg tgaagcaaac tctaagatcc aagctgattt ggcagaaaaa gaattgaaaa
    51661 aagcgggcgt caaagtcaaa gtactgacag caaattccac aaatgatgtc caacaagtga
    51721 cgaaaagtct agctaaagat gtagatggaa tctatattcc tactgataac acgttcgcaa
    51781 gtgcggcaag tgttgtcggt gaagtcgcaa aagaaacaaa aacacctgtc gttgctggcg
    51841 cagttgatca agtcaaagac ggcggtctag caacagtagg aatcgactac gaaagcttgg
    51901 gcaaacaaac agggatcatg gcagcaaaaa tcttagacgg caagaatcca agtgaacttc
    51961 ctgtcgaaac agcgaatgat ttggaactgt atgtcaataa agacatggcc aaagcattag
    52021 gcatcaatcc agacagcatc aaagaagctg aataaaacac aggaggattc tcatgacaaa
    52081 tttgctctct cttttcttat catctgcatc gcaaggggtc ttatgggccc tacttgcgat
    52141 tggtgtatac gtcactttcc gtatcttgga cattgccgac ttgactgctg aaggaagctt
    52201 tcctttaggt gcagggatcg cggcagtaag tattacaaat ggctatcatc cattgacagc
    52261 ttgtcttcta ggctttttag ggggtgcggc agcagggctt gtttccggac tgcttcacac
    52321 aaaattgaaa attcccgccc ttcttgcagg gatcatcacc atgactggtt tatattctgt
    52381 cacatcccgt gtgatgggtg cgccgaacat ctctttattg ggtcagccaa cgatctttca
    52441 ttgggtcgag tcattcggca taacaaaaga caacgctgta ctgatcgtcg gtctgctttt
    52501 tgctttgatc gttgtatgtt tacttgtttt gttcttacgt acagaaacag gacttgctat
    52561 tcgtgcaact ggcgataatc tggcaatgag cgaagcaaac gggatcaaca cagataatat
    52621 gaagatcatc ggttatatga tttctaatgg atgcattgct ctttctggct ccttacttgc
    52681 gcaaaacaac ggctttgctg atttaaattc tggtattgga acgatcgtaa ttggacttgc
    52741 ttctattatc atcgcagaag ttttgttacg taaccagcca ctatttctcc gtctcttgac
    52801 tgtcgtttta ggtgcggtca tttatcgttt tattttagcg ttagtcttcg aactgaatgt
    52861 tgaaccaagt gattcgaaac ttgcttctgc tttcgtgcta gtgatctgtc tttctttccc
    52921 gcagatccag caaaaattcc atttacctca aaaaaagaaa ggcggtagtg tcaaatgaat
    52981 gacgtcttaa cattggaaaa tctccatcat acctttgaaa aaggaacgat caacgaaaat
    53041 catgtcctga aaggcattaa tctttcctta gatcccggcg acttcgtgac gatcatcggt
    53101 ggaaatgggg ctggtaaatc tactttgctg aacagcatcg ccggtacatt ttctgtcgat
    53161 cagggtcaaa ttttattgaa tggaaaaaat atcacgaaaa aatctgttgt cgaacgttcg
    53221 aaatccatca gtcgcgtatt ccaagatcct aaactaggga cagccgttcg tttaacggta
    53281 gaagaaaatc ttgctcttgc tatgaaacga ggaaaaaaac gcggcttctt ccgtggtgtc
    53341 aaaccacaag accgaacatt tttcaaagac cagctggttc ggctaaattt ggggttggaa
    53401 aatcgtttat cctcagaaat cggtttgctg tctggaggac aacgtcaagc tattacgtta
    53461 ttgatggcca cattacagcg tccagaactg atcttattag atgaacacac tgcagcgctt
    53521 gaccctgcta cttccaaaac ggtgatggaa ttgacagaaa agctgatcac tgagcagaat
    53581 ctcactgctt ttatggtcac tcatgacatg gaagacgcga tccgctatgg gagtcgactg
    53641 atcatgctgc atcaaggaag gatcgtcgta gatatccagc aagaggaaaa acagcagctc
    53701 acagtgcccc aattattagc gttgttcaaa caaaacagcg gcacggcatt aaaagacgat
    53761 caagtacttt tatcttcata atcagaaaga ggatgggaca aaaaatgaac gcccaccctc
    53821 ttcttttttc tctttccttc tttcgttttt gctttatgta tcaaccgttg tttcttcgaa
    53881 aactattttg ttctctcctt cttcctgata gaatggatgg gaaaggagct tgatgccatg
    53941 acaacgaaag acaaggtact tcaattattg aaaaagacac cagattttct ttccggagaa
    54001 aagttggcac agcaactgga agtttcccgg acaagtattt ggaaagccat caaagaattg
    54061 gaaaaggcag gctatcgttt tgagcacggg cctacagggt atcgttacct gccctctgac
    54121 gtactagatg caaaagaaat ccagcaagag ctacggtcgt ttattcctga tttgaaagta
    54181 caaacgatgt tgacttctga atctacgatg aaagatgcca aactggcagc agctcaagga
    54241 gattctgttt cgaagttatt tgttgctgat acacaagaac aagctcgtgg acgttttgga
    54301 cggccttttt tcgctgaacc tggacgagga atctacatga gtctcttgct ccatccagac
    54361 aaacggtttg atgaattgcc tcaatatacg atcatcgcag ccagtgcctg tgtgaaagcc
    54421 attgaatctg taaccggtcg ttctgcttcg atcaaatggg tcaatgatat ctacatggat
    54481 ggaaaaaaag tctgtgggat tttatctgaa gcagtcagtg atatggaaag cggcaggatc
    54541 agtcatatca tcattgggat cgggcttaat ttttctgttc ctcaaaatca ttttccagct
    54601 gatctccaag aaaaagccat ctctgtcttt cccgacggac aagctagtgt cacaaggaat
    54661 cagctaatca gcaaaatctg gcaagaattg ttcacttatc tttctcaccc agaaaaagcg
    54721 ttggacttct ataaagaaaa atcattcgta ttgggaaaaa ccgtgtcgtt caatcaaaaa
    54781 ggaacgactt atgtcggtct tgcaaaagaa atcggtgatc atggcgaact gatcgtccaa
    54841 acaaatgaac agaagatgag tttgtcttct ggcgaaatca gtctcacatc tatccgtacg
    54901 caataattat cagatttcac aaaaggacac ctgatctcat acagtgtcct tttttctgaa
    54961 ttctcttcaa aagactctcc ttattctatc agctgacagt ttctctatcc tctagaaaaa
    55021 ggtattctaa aaatatctat aaagcatggt acactttctt taaatgaatc aatgcaaaaa
    55081 acaagagagt catcagttta cacgatcaaa agagatagca ggccgcttcc tttttcaatc
    55141 ttatagatag accatgggta acgataaaaa caaaaaagaa cttgcgactt attttgccgt
    55201 aagttctttt ttagctagtg ccttaatctc ttaatcccaa caaacgtctc gcatagatca
    55261 cctgaccaga gtgcatagca gcatcatcaa taatagaaac gagacgcatt cctcgtgtga
    55321 ctggtggtgt ccagttctcg tcaattactt catctagctc ggaaggttcc agccttctta
    55381 agtaagaaat agcaaaatca gcagctcctc gcaagtatcc tagcagatct tctatttgat
    55441 tagcttggat ctgctttgct tgttccagac tatgcttcca atcttgttcg ttatctgcta
    55501 tcgtcatcgg aaaccggctt tcccagtctt gactatgcca aattggagaa gttcctgcta
    55561 aatcagcaat ctggaaatct aattctcgag cggtatgcca agccagccaa gtcattgatt
    55621 tgatctgcgg ggctctatct tccactggaa aacgattggc ctcctcaata gagacgcctt
    55681 caaacaaacg ttcaaaccga tcgtatgccc gatccacact ctcgcatagc atatctaaat
    55741 aatccattta ttttcctcct ttttatttat cctacttact ttttcctaat tctaggatac
    55801 ctacttatat gttactacac acccttattt tttacacttt aacagtcacc atcatcaata
    55861 gtttggagat tgaaagaaaa agatagtgtc gaactatcaa ttgaacaaga agcaacaata
    55921 tgaacgtttc attaatattc tttaaataaa caatgatttt tcaaacgaac agtttaagac
    55981 taggagaaaa agagtataat aggaaaatgt ttgaaaggaa aggaagggga aaaatgaaaa
    56041 aaagggcaag tttactagtc ctagttgctt tgttgttcaa tatcttttcg tatggtgtgg
    56101 ccgtttcagc ggaaacatac attcctaaag accctgggaa tatttataat gatctaactg
    56161 gtaactatgg cttgttagga attgctagtc aattccacgt atttgctaaa aataagacaa
    56221 ctgtgaatgc acatacagac ggaaatatag caacacatga acttgatgca aaaaataatt
    56281 ttggtacaga gcttcatgaa ggtttattga accaagaaat aaattatata caaaaaactg
    56341 attctttcgc taattcttca ggcataccta caggtagcac aagaatgaat aaatttgtcg
    56401 ttggcagtaa cgtggatgta gcacttcaag atggtcatcc tacagtcaat ggatccaggc
    56461 ttgatcactt aactccagat gatttttata aagatcgtgc tgggcatgaa tatattgatt
    56521 tcgagcagga atttgctaaa ttgaatgtag cagcaaatga tcttgcaacg atcactccag
    56581 ctaaaacata tactgctgca gattttccag acatgaacaa tcggacaatc gatttaactg
    56641 atttatcaga taccgggttt ttattggtaa atatcgatgc agaagtgtta acgatgaata
    56701 cgccacttca aatcattaat ccaaatgatc aagtagttgt atttaatgtc atcaacagtt
    56761 cttctgcatt gaacgtacag tcaccaatca aatacaatga tcgttcaaat catgaaacag
    56821 aagatttttc ggatgcgaat atctcttgga attttggaaa tgagatgact gatttgacaa
    56881 tcagtgctcc ttttcaagga acaatccttg cgcctaatgc tactatccgc attactcaaa
    56941 accaagacgg aacgattata ggaacaaatg tgattttaga tgcagctacc aatcgttggg
    57001 atcctaacga aatatttatc agcgacaatg gtacagatac gacagacaca agcgacagta
    57061 cagatacatc taccacaagt gatagctcag attcatcaac ttcaagcgac agtacagata
    57121 ctacctctac cacaagtgat agcacggata catcagcttc aagcgacagt acagatacta
    57181 cctctaccac aagtgatagc acggacacat c
//
LOCUS       DARBFO010000009        51846 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000009 DARBFO010000000
VERSION     DARBFO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.009"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            176..904
                     /locus_tag="SCK51_000688"
     CDS             176..904
                     /locus_tag="SCK51_000688"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEG1251335.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            904..1158
                     /gene="purS"
                     /locus_tag="SCK51_000689"
     CDS             904..1158
                     /gene="purS"
                     /locus_tag="SCK51_000689"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEG1251336.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1160..1831
                     /gene="purQ"
                     /locus_tag="SCK51_000690"
     CDS             1160..1831
                     /gene="purQ"
                     /locus_tag="SCK51_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEG1251337.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1832..4054
                     /gene="purL"
                     /locus_tag="SCK51_000691"
     CDS             1832..4054
                     /gene="purL"
                     /locus_tag="SCK51_000691"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEG1251338.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4039..5478
                     /locus_tag="SCK51_000692"
     CDS             4039..5478
                     /locus_tag="SCK51_000692"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEG1251339.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5501..6553
                     /locus_tag="SCK51_000693"
     CDS             5501..6553
                     /locus_tag="SCK51_000693"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEG1251340.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6550..7128
                     /gene="purN"
                     /locus_tag="SCK51_000694"
     CDS             6550..7128
                     /gene="purN"
                     /locus_tag="SCK51_000694"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEG1251341.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7125..8660
                     /gene="purH"
                     /locus_tag="SCK51_000695"
     CDS             7125..8660
                     /gene="purH"
                     /locus_tag="SCK51_000695"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEG1251342.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8676..9950
                     /gene="purD"
                     /locus_tag="SCK51_000696"
     CDS             8676..9950
                     /gene="purD"
                     /locus_tag="SCK51_000696"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEG1251343.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10072..10986
                     /locus_tag="SCK51_000697"
     CDS             10072..10986
                     /locus_tag="SCK51_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1251344.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11119..12684)
                     /locus_tag="SCK51_000698"
     CDS             complement(11119..12684)
                     /locus_tag="SCK51_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG1251345.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12677..14128)
                     /locus_tag="SCK51_000699"
     CDS             complement(12677..14128)
                     /locus_tag="SCK51_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1251346.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14142..15158)
                     /locus_tag="SCK51_000700"
     CDS             complement(14142..15158)
                     /locus_tag="SCK51_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1251347.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15378..16466
                     /locus_tag="SCK51_000701"
     CDS             15378..16466
                     /locus_tag="SCK51_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEG1251348.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16520..18046
                     /locus_tag="SCK51_000702"
     CDS             16520..18046
                     /locus_tag="SCK51_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEG1251349.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18048..19253
                     /locus_tag="SCK51_000703"
     CDS             18048..19253
                     /locus_tag="SCK51_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1251350.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19510..20487
                     /gene="guaC"
                     /locus_tag="SCK51_000704"
     CDS             19510..20487
                     /gene="guaC"
                     /locus_tag="SCK51_000704"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEG1251351.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            20751..22175
                     /locus_tag="SCK51_000705"
     CDS             20751..22175
                     /locus_tag="SCK51_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEG1251352.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            22636..23745
                     /gene="pdhA"
                     /locus_tag="SCK51_000706"
     CDS             22636..23745
                     /gene="pdhA"
                     /locus_tag="SCK51_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEG1251353.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            23748..24725
                     /locus_tag="SCK51_000707"
     CDS             23748..24725
                     /locus_tag="SCK51_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEG1251354.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            24750..26393
                     /locus_tag="SCK51_000708"
     CDS             24750..26393
                     /locus_tag="SCK51_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEG1251355.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26401..27807
                     /gene="lpdA"
                     /locus_tag="SCK51_000709"
     CDS             26401..27807
                     /gene="lpdA"
                     /locus_tag="SCK51_000709"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEG1251356.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            27969..28244
                     /locus_tag="SCK51_000710"
     CDS             27969..28244
                     /locus_tag="SCK51_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEG1251357.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28250..29017
                     /locus_tag="SCK51_000711"
     CDS             28250..29017
                     /locus_tag="SCK51_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEG1251358.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29185..31014
                     /gene="typA"
                     /locus_tag="SCK51_000712"
     CDS             29185..31014
                     /gene="typA"
                     /locus_tag="SCK51_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEG1251359.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31275..33047
                     /gene="recQ"
                     /locus_tag="SCK51_000713"
     CDS             31275..33047
                     /gene="recQ"
                     /locus_tag="SCK51_000713"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEG1251360.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33161..33463
                     /locus_tag="SCK51_000714"
     CDS             33161..33463
                     /locus_tag="SCK51_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEG1251361.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            33466..34629
                     /locus_tag="SCK51_000715"
     CDS             33466..34629
                     /locus_tag="SCK51_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEG1251362.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            34655..38083
                     /locus_tag="SCK51_000716"
     CDS             34655..38083
                     /locus_tag="SCK51_000716"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEG1251363.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38104..39228
                     /locus_tag="SCK51_000717"
     CDS             38104..39228
                     /locus_tag="SCK51_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251364.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39218..39655
                     /locus_tag="SCK51_000718"
     CDS             39218..39655
                     /locus_tag="SCK51_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG1251365.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            39639..39977
                     /locus_tag="SCK51_000719"
     CDS             39639..39977
                     /locus_tag="SCK51_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEG1251366.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40096..40656
                     /gene="rsmD"
                     /locus_tag="SCK51_000720"
     CDS             40096..40656
                     /gene="rsmD"
                     /locus_tag="SCK51_000720"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEG1251367.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            40653..41144
                     /gene="coaD"
                     /locus_tag="SCK51_000721"
     CDS             40653..41144
                     /gene="coaD"
                     /locus_tag="SCK51_000721"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEG1251368.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41134..42177
                     /locus_tag="SCK51_000722"
     CDS             41134..42177
                     /locus_tag="SCK51_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEG1251369.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42336..43064
                     /locus_tag="SCK51_000723"
     CDS             42336..43064
                     /locus_tag="SCK51_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEG1251370.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43180..43425
                     /locus_tag="SCK51_000724"
     CDS             43180..43425
                     /locus_tag="SCK51_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG1251371.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            43422..43640
                     /locus_tag="SCK51_000725"
     CDS             43422..43640
                     /locus_tag="SCK51_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEG1251372.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            43776..44315
                     /locus_tag="SCK51_000726"
     CDS             43776..44315
                     /locus_tag="SCK51_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEG1251373.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44308..46581
                     /locus_tag="SCK51_000727"
     CDS             44308..46581
                     /locus_tag="SCK51_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEG1251374.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            46619..47665
                     /gene="holA"
                     /locus_tag="SCK51_000728"
     CDS             46619..47665
                     /gene="holA"
                     /locus_tag="SCK51_000728"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEG1251375.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(47754..48008)
                     /locus_tag="SCK51_000729"
     CDS             complement(47754..48008)
                     /locus_tag="SCK51_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEG1251376.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48408..48677)
                     /locus_tag="SCK51_000730"
     CDS             complement(48408..48677)
                     /locus_tag="SCK51_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251377.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49147..49602)
                     /locus_tag="SCK51_000731"
     CDS             complement(49147..49602)
                     /locus_tag="SCK51_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEG1251378.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(49740..51668)
                     /locus_tag="SCK51_000732"
     CDS             complement(49740..51668)
                     /locus_tag="SCK51_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEG1251379.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        17056 a         8906 c        11323 g        14561 t
ORIGIN      
        1 tgttgatggt ataagatttt cgtttctgat aaaacacgaa cattaaaaga atctaatgcc
       61 ttaaatgtac atatttttgt tgatttataa aaagatgatt gctaaaattt aaagtgaaaa
      121 aacgaacaaa aaacgatgta tttgctttta atgtaaataa aaaggagaga cgtacatgga
      181 gaagaaagaa cttttgtatg aaggaaaagc caaaaaaata tatcttactt ctgatgagaa
      241 cttgatttgg atagaatatt tagatcaagc aactgcgttg aatggcgcga ggaaagacca
      301 gataagtgga aaaggagaat tgaataatca aatcacaagt gcaattttcc gttatctgac
      361 tcaaagagga ataaaaaatc attttattga acaattgtct aaaaccgaac aattagcaaa
      421 aaaagtacaa attatccctc tggaagtggt tatccgaaat attgcagcag gaagtttctc
      481 aaaacgtcta ggtgttcccg aaggtaaaga attcatcaaa ccaatcattg aattctacta
      541 taaggatgac cagttggatg atccatttat caatgaagag catatctatt tcttacaact
      601 tgcaaaaccg gaagaaatca gtgagctgaa agcccaagcg ttggctatta atgaaatttt
      661 gaccgaatta tttgctaagg tcggtttggt tttagtagat ttcaagctag aatttggccg
      721 gtcgaatgga caaattttgc ttgcggatga agtaacaccg gatacttgcc gattatggga
      781 tgaacgtacg aatgaacata tggacaaaga cgtttaccgc agagatctag gtgatttgat
      841 cccagtctat caagaagtat ataaccgtct atcggaacta actgaagaaa aggagcgagt
      901 atgatgtatt tcgtaaaagt atatgtaacc tataaagatt cagtgttaga tcctcaaggg
      961 gaggcagtaa aaaaagcagt ccatcgtatg ggatatgaaa caatcgaaga tatccgaata
     1021 ggaaaatact ttgagataca acttgctgat gcagaagaac caatcgatac agtgattgaa
     1081 acgatttgtg acaagctgct tgcaaatgta aatatggaaa cataccgata tgaaatcaaa
     1141 aaaatggagg aagtataaca tgaaatttgc ggtcatcgtt tttccaggtt caaattgtga
     1201 cgcagatatg ttatgggcag ttcgcgagat catgggcgct gatgccgaat ttgtacggca
     1261 tgatgagtcc tctttagaag gatttgatgg ggttttactg ccgggaggat tctcctatgg
     1321 tgactatttg agatgcggtg cgattgctcg gttttctaca atcatggaag aagtgattcg
     1381 ttttgctgaa gaagggaaac cagtattcgg tacatgcaat ggattccaga tattgacaga
     1441 agcaggactt ttgccaggtg tactgagacg aaatgagtct ttgcatttta tttgcaaaac
     1501 tgtaccattg aaagtcaacc ccaagacggc ctttacttca gagtatgaag ccgacgaaat
     1561 aatctatctt ccagttgctc atggggaagg aaattatttc tgtgacgaag aaacacttca
     1621 agaactaaaa aaaaataatc agattgcctt tacttataat gacaacatca acggcagcgt
     1681 ggaaaacatt gcagggatta cgaacaaagc agggaatgtg ttaggaatga tgcctcatcc
     1741 agaacgtgca gtagaagact tgctaggatc agaagatggg aaacggttct ttgcttccat
     1801 tttaaaaaac ttcggaaagg tgaccgtata gatgaaaaca ttgaaagaac cgacagctga
     1861 agagatcaag aaaaaaagaa tctatgccga atggggattg actgatgaag aatatcgaat
     1921 gatcgaagaa gatatcttag gaagaatgcc taattatacg gaaactggac tgttttctgt
     1981 tatgtggagc gaacattgtt cctataaaaa ttctaaaccg gttctacgca aatttcctac
     2041 tagtggacct catgtgttgc aaggccctgg agaaggcgca ggcatcgtcg acatcggtga
     2101 tggactagca gttgtcttca aagcagaaag tcacaatcat ccttcagccg tggagcctta
     2161 tgaaggagct gcgacaggag ttggcggaat catcagagat attttcagca tgggggctcg
     2221 tccaattgcg ttgctagatt cattgaggtt cggcgaatta gataatccaa gaacgaaata
     2281 tttactagaa gaagtagttg caggtatcgg cggatacggc aattgtatcg gcataccgac
     2341 tgtaggcggc gaaattgcat ttgatccttg ttatgaagga aacccattag tcaatgcaat
     2401 gtgcgtaggt ttgattgaac acaaacatat ccagaaaggg caagccgctg gcgtcggtaa
     2461 ttcgatcatg tatgtaggtg caaaaacagg tcgcgacggt atccacggag caacttttgc
     2521 ctcagaagaa tttaatcaag aagaagaaca gcaacgttct gctgtacaag taggcgatcc
     2581 ttttatggaa aagctgctgc ttgaagcgtg tttggaatta atcttgcatc attcggattc
     2641 tctaataggt atacaggata tgggagcagc aggacttgtt tcatctagtg cggaaatggc
     2701 ttcaaaagct ggctcaggac ttatattgac attagatgaa gtcccacaaa gagaaactgg
     2761 gatgacacct tatgaaatga tgttatcgga atcacaagaa cggatgttga tttgtgttaa
     2821 atcaggagag gaagaaaaaa tccaagaact atttcagaag tatgatctag atgcagtcac
     2881 tatcggtaaa gtaacagacg acgggcaata tcggctatat catcaaggag aagaagtagc
     2941 aaatctgcca gtcgatgcat tagctgaaga tgcaccagtt tatcataaag aaatgaaaga
     3001 accagctcga atcgtagaat ttcagcagtt agctccttat cagccagtta tcgaagaacc
     3061 tggacaagta ttgcttgatc tgctgcagca accgacgatt gcttcaaaac gttcaatcta
     3121 cgaaacttat gattcacaag tacaaaccaa tacagttgtc cgtccaggaa gcgatgctgc
     3181 tgtgatgagg gttagaggga cgaataaagc gttagctatg accacagact gcaacgcgcg
     3241 ctatctgtat ttggatccaa aaatcggagg acaaatagca gtggcagaag ctgctagaaa
     3301 tattattgct agcggtggta agcccttggc gatcaccgac tgcctgaact atggttctcc
     3361 tgataaacca gaagtttttt gggaattatc gacttcagct gacggaattg ctgctgcgtg
     3421 tgaaacgtta ggaactcctg ttatttcagg aaatgtctct ttatataatg aaacggatgg
     3481 tcaagcaatc tatccaacac caatgattgg tatggtcgga ttgattgagg atcataaaca
     3541 tatcactact caggaattca aaaaatcagg ggatctaatc tatatattag gtaagacgtt
     3601 tgcagatttt gatggcagtg aattacaaaa gatgcagctt ggaagaatcg aaggtgtgat
     3661 ccgaaacttt gatctatcca ttgaaaagag aaaccaagag ttagtattga cagccatcca
     3721 aaacggattg attgaaagtg ctcatgactg ttcagaaggt ggcttggcaa ttgcactggc
     3781 agaatcagct ttcaaacatc agctagggat atctgtacag tttgaattgt cctctgctca
     3841 attatttgca gaaactcaat caagatttgt gttgacagtc gcaccggaaa ataagacacg
     3901 attcgaagaa atgatgggag atgctgctgt tttagcaggt aaggtgacgg atgaagcgat
     3961 aatcgaaatt tcagcaactg acggtcagat caaaatagaa actgcagtag caagaaagtg
     4021 ctgggaggat gcaatcgtat gtcttatgaa gtgaagagtt taaatgaaga atgcgggatc
     4081 ttcggtgttt ggggtcatcc agacgccgct agtgtggcat acttcgggct ccatagtctt
     4141 cagcatcgag gacaagaagg tgcaggtatc gtgtccaatg atcgaggaaa gcttaagggg
     4201 catcgagatt tggggctgct ttctgaggta ttcaaagatc aaagaaaatt gcagcagtta
     4261 acaggagaag ctgcaatcgg acatgtacgt tatgcaacag caggcaatgg aagtgtggac
     4321 aatattcagc cttttctatt caaatttcat gatcagtcga tcggcttagc gcataatggt
     4381 aacttgatca atgctcgttc ccttagaaaa gagttagagg aaaatggggc gatcttccat
     4441 tctaactccg atacggagat attgatgcat ttgatccgac gaagtgaaga aacagatttt
     4501 ttaaataaat taaaaagtgc tttgaatgaa gtcaaaggtg gctttgccta tttattaatg
     4561 acagaaacgg caatgattgc cgctttggac ccaaatggtt ttcgtccatt agcaatcgga
     4621 caaatgaaaa acggagcata cgtgattgcc tcggaaactt gtgcattaga agttattggg
     4681 gcacgtttta taagagatgt ccagcctggt gagatagtga tcatcaatga cgaaggaatc
     4741 cagattgatt gctttacgaa ggacacgcag ctttctattt gttcgatgga atatatctat
     4801 tttgccagac cagattcaaa catcgcaggg atcaatgtgc atactgcacg gaaaaatatg
     4861 ggacggaatt tagcgaaaga atcgccagta aaagcagata tggtcgttgg cgtacctaat
     4921 tcttctttgt cagccgcaag cggttatgcg gaagctagtg gaatccctta tgaaatcgga
     4981 ttagtaaaaa atcaatacat tgcccgaaca tttatccagc caacgcctga gctaagagag
     5041 caaggtgttc gaatgaaatt atctgctgta cgaggagtag tggaaggtaa aaaggtgatt
     5101 atggtcgatg attcaattgt tcggggtacg acaagcagac ggatcgtcca tcttttgaaa
     5161 gaagcagggg caaaagaagt ccatgtgagg attgcttctc ctcctttgaa atatccatgt
     5221 ttttacggga ttgatattca aacgcgtaaa gaactcattg cggcaaatca cacgattgat
     5281 gaaatcaaag aatgtatcgg tgcagattct ttaggttttt taagtgaaga gggtctgatc
     5341 gaatcgatcg gtctggaaaa agatgctccg tattcaggat tgtgtatggc ttatttcaac
     5401 ggagattatc cgacgccgtt atatgactat gaagaaaaat atcaggaatc actaaaagaa
     5461 aaagtatcgt tcttttaaaa gcagaccaag aagggagtat atgaattcaa tggcaaatgc
     5521 atacgcaaaa gctggcgtag atgtcgaagc tggatatgaa gtagtagaac ggatcaagaa
     5581 gcatgtcaaa aaaacggaac gtttaggtat gatgggcgct ataggaggat tcggcggttg
     5641 ttttgattta tcggtctttc aattaaaaga gccagtcctt gtctcgggga ccgatggcgt
     5701 ggggacgaaa ttagcagtgg caatcgaagc agacaaacac gatacaatcg ggatcgactg
     5761 cgtagcgatg tgtgtcaatg atatcttagc acaaggcgca gagccgttat atttcttaga
     5821 ctacttagca acggggaaaa atgatccgtt acgtttggaa aatgtagtaa aaggtgtagc
     5881 agatggatgt gttcaaggaa atatggcgct gattggcgga gaaacagctg aaatgcctgg
     5941 tatgtactca ggtaatgatt atgatctagc cggatttgct gtagggatag ctgaaaaatc
     6001 tcaattattg acaggagaaa cgatcaaccc aggagatgtg ttgcttggaa tagcatctag
     6061 cgggattcat tcaaatgggt actctcttgt tcgcaaaatt ttctttgagc agcagcagtt
     6121 ttctttatca tcaaaactgc cagaattgaa aaaaacactt ggcgaagaat tgcttgagcc
     6181 aacgaaaatc tatgtttctt ccttgcttcc gctgatcaaa gaaaagaaaa taaaaggagc
     6241 tgcacatatc actggtggag ggtttgtaga aaatatcccg cgaatgttac cagaatctat
     6301 ggctgcacaa atcaaacttg gctcatggcc gattcttcca atattcggct gtttacaaaa
     6361 atatggtgaa attcctgaga tggagatgta tgaaatcttc aatatgggca tcggtatggt
     6421 cctggcagtc gaagaatcga aagtaaaaga aatagtacac gaaattgagc aagctggcga
     6481 aaaagtttat gaaatcggga aagtgatcgc caaagaaaaa catcgtgtgg tgtttatagg
     6541 agaagaacga tgagaatcgc tgtttttgct tctggaaacg gaagcaactt tcaagcgcta
     6601 gccgattatc tatcgaaaaa aggattggaa tcttcgattg actggttgtt ttgtgatcag
     6661 cctgaagcat atgttttgaa acgtgcaact gcgttgagtg taccggcaga ttgtttttca
     6721 cctaaagaat ttgattcaaa aaaagagtat gaagaagcaa ttttgcataa attgaaagaa
     6781 aaaaagatcg atttgatcgt attagctgga tatatgcgaa tcattggacc ggtattgctg
     6841 aaaaattatg ataaaagaat catcaatatc catccgtctc ttttacctgc cttccctggt
     6901 ttgcatggca tacgtgatgc ctttgaagct ggagtaaaag aaacgggtgt cacaattcat
     6961 tatatcgacc aaggtgtaga tactggacca atcatccggc aggaaaaagt gagaattgaa
     7021 caggaggaca cgttcgattc tttagaagaa aaaatccata gagtagagca tcgaatctat
     7081 ccagaagtga ttagtgaaat tattgaaaat ggaggctatc tgaaatgaca agagcattga
     7141 tcagtgtatc agataaaact gggattgtag cattcgccca aggtttgatt gaacaaggag
     7201 tagaaatcat ttcaacagga ggaacaaagc atgcgttgga taaagctggg atcaaaacgc
     7261 tttcgatcga agaagtgaca gggtttcctg aaatgatgga tggccgtgtc aaaacacttc
     7321 atcctaaaat ccatggtggt cttttaggaa gacgcgatct ccctagccat atggaggcaa
     7381 tggaaacgga aaatatccaa ccaatcgact ttgtttgtgt caatttatat cctttcaaag
     7441 aaacgattat gaaaccagat gtcgaaatag cagaagctat tgaaaacatt gatatcggtg
     7501 gtcctagtat gctccgtagt gcagcaaaaa accatgaatt tgtcacctct gttgttgatc
     7561 catctgacta tgaagaagta ctttcagaat tacgtcagta tgggcatact tcactttctt
     7621 tcagacaaaa actagcggca aaagtctttc gtcatactgc ggcatatgat gcactgatcg
     7681 ctcaatactt aacggattgg gtggaagaaa aagaaccgga aaaattgaca cttacgtatg
     7741 aacgaaaaca aacgcttcgt tacggtgaaa atagccatca agccgctgcc ttttatcagt
     7801 ccgctcttct ggaaaaatat tcgatcgcag cagcaaaaca actccatggg aaagaacttt
     7861 cttataataa tatccgagat gcagatgctg cgctacggat catgaaggaa tatgacgagc
     7921 caacggtagt cgccttgaaa cacatgaatc cttgtggtat tgggacagca tctacgattt
     7981 tagaagcgtg ggaagcagcg tatgaagcag atcctgtttc aatctttggt gggatcatcg
     8041 ttttgaatcg agaagtagat ctgccaacag cagaacagat gcataagctg tttctagaaa
     8101 tcatcattgc cccttcttat acacaagaag ctttggaatt actgcaaaaa aagaaaaaca
     8161 tccgattact gacagtagac tttgcgcagg aagaaaatca aaatcgagaa gaaacggtct
     8221 cagtcttagg tggacttctt gtacaagatc aagatctggc aatagaaaat gaagaagagt
     8281 ggaaagtcgt gacaaaacgt aagccgactc cagcagaagc aaaagcatta gcgtttgctt
     8341 ggagagctgt caaacatgtg aaatctaatg cgatcgtact ggcaaacgaa cgtcagacag
     8401 taggaatcgg agccgggcag atgaatcgag tcggttcagt aaaaattgcg attgaacaag
     8461 cgcaagagaa aatggaaggt gcagttttag ctagcgatgc ttattttcca atggatgatt
     8521 cggttgaata tgcagccaaa catggtataa aagcaatcat tcagccagga ggaagcatca
     8581 aagaccaaga ttctattgat atggcgaata aatacgatct agcaatggtc tttactgatg
     8641 ttcggcattt tagacattag agaggagtcg gataaatgaa cattttggtg atcggtgcag
     8701 gtggtcgtga acatgcaata agtaaaaaac ttttagccag tccgaaggtg gatacggtgt
     8761 attgtgcgcc aggaaatccg gggatgacaa aagatggtat caattgtgtc gctatcagtg
     8821 agcgagatca ttcagcgtta atccattttg ttaagaaaga ggctattgat tggagtatcg
     8881 ttggtcctga agttcctttg ttaaatggga tcgtcgatga ttttatggcg gaaggattga
     8941 aaatcttcgg tccgacaaaa gcagcagctc tgatcgaagg ttcgaaaagt tttgctaaac
     9001 aattaatgga tacttatcaa atcccgacag ctaaatccag aagttttgaa tgttacgaaa
     9061 aagcagcagc gtatatcaaa gaaaatggtg cgccaatcgt agtgaaagcg gacggcttag
     9121 cagctgggaa aggtgtagtc gtggctacca cagtgaagga agcgctggat gcggcagaac
     9181 aaatgttgaa ccagcatcga tttggggata gtagcaaaaa aatcgtcgta gaagagtttt
     9241 tggcaggaga agaattctcc cttttggcgt ttgtccgtaa taccaaggtg tatccgatgg
     9301 tcatttcgca agatcataag cgggcatttg ataacgataa agggccaaat acaggcggaa
     9361 tgggtgctta ttcaccagta ccgcaaattt caaaaaagat gattgaaaaa gctgtggaag
     9421 aagttttaaa accagcggct ttgggaatga aagaactagg acgctctttc acgggtgttt
     9481 tatatgcggg tttgattgct acgaaggaag gaccaaaagt gatcgaattc aatgcgcgtt
     9541 tcggtgatcc tgaaacgcaa gtcgttcttt ctcggttgaa gagtgacttt gcgcaagtaa
     9601 tcgatgattt attagagaat cgtacagtag agttaaagtg gcagcaagaa ggatacaatg
     9661 taggcgttgt ggttgcagct gccggttacc ccgaagaata tgagacagga atggttctac
     9721 cagattttct ggaagaagag ctttctgttt actatgctgg agtttcctca aataacggca
     9781 aactagttgg agcaggagga cgtctttatt tagtagaagc atacggtgag acgatcaaag
     9841 aagcacagaa aaaaatttac accgcgttaa tggataaaac gacagatgga acattttatc
     9901 gtacagatat tggagaaaaa gctgccaata gcgaactgat cgtgaagtaa atcagctgct
     9961 tccatttata aaaaatacaa caaacataat cggaagcgcc gattttagtg aagccttttc
    10021 ataaagagtg tggtacactt ttgttgtgta tataaataag gaggagttca gatgaaaaaa
    10081 attcaattag gtacttctga tcagaaagca gctgcagtga tcttaggctg tatgcgtatc
    10141 aacaatgcgg aagatccagt aaaagtgatt gagacagctt atgaaaacgg cattgatttt
    10201 tttgaccatg cagatattta tggaaacggt gaatgtgaag aaatctttgc agatgctcta
    10261 gcaaaaacat caatcaaacg agaagatctt tttattcaaa caaaatgtgg aatcgttcca
    10321 ggtgtgatgt ttgatttttc taaaaaacat atcattgaat ctgtagaaaa aagtttgacc
    10381 cgtctgaaaa tggattatgt ggatgcttta cttcttcatc gtccagatac attggtagaa
    10441 ccagaggaag tagctgaagc gtttgatgaa ttggaacggt caggaaaagt tcgttatttt
    10501 ggcgtcagca atcaaaaacc aatgcaaatc gaattgctga aaaaatatgt tcgtcaacca
    10561 ttagtagcta atcagctgca atttggcttg aaacatactg gaatgatcga tcaagggatt
    10621 catgtcaata tgacagatga agcaagtatc gatcatgacg gtagtgtact tgattattcc
    10681 cgattgcatg atatgacgat ccaagcatgg tcaccatacc agtatggatt ttttgaaggt
    10741 gtatttatcg ggaacgaaaa attccctgaa ttaaaccaaa cattagaaga actagcagct
    10801 aaatatcaaa cgacaccaac tggcttggct tctgcttgga ttttacgcca tccagccaat
    10861 atgcaagtga tcgccggtac gatgaacgta gggcgaatca aggaaatcgc tcaagcttca
    10921 gaaattaata tgtctcggga agattggtac gaattgtacc gtgctgctgg aaacatttta
    10981 ccttaaagat tgtcataagc aggtcaaaag ggctataaat taaaaagaca gaataacttg
    11041 aaagctgttt tcagcttcga gatattctgt ctttttttat tgcaaatgga aagagtatca
    11101 ttgtacggat ttcgtttatt agttattttt gtttttaagg aaaagctgtg tccgatattc
    11161 ttttggagaa atcccttgtt ttttcttaaa taaataactg aaatagttag ggtcattata
    11221 accaatttca aaagcaatat ctgaaatacg atagtctgat tgctgcaata atttttttgc
    11281 taattcgatt ctttgttctg tcagataatc gatgaatgtt tggttaaacg attgagaaaa
    11341 aatcgtactg aaatgagagg gacttaatgc aacttgttga gcgacccaag ttaaggacat
    11401 atctggatcg gtaaaatttt ctttgataaa atccaatgca tggttcaaaa ttggctgata
    11461 tttgttcctt gaaggctgtg agggcagagc agttaagaaa gaaatgaaca tataaactgt
    11521 cgaacggtaa agcgagtcgt cggcgctgat tgctaagcgt tgactggtgg atgtgcaatg
    11581 aaccatttct tgcgggattt tcttttcttt caggcagaca tgctggagta gcttagtcaa
    11641 gacaaataaa cgatataatc gtgttcgagt tggtgtgcct tgtggtttcg tcaagtgttc
    11701 gatgtactta gatacttcgt ttttcgataa tgaagacaaa tcaagtgaga ggtcaaagta
    11761 aaagacagaa tcaactggaa gtttcgcttc ttgaatcaaa tcctcatagc tgataatttg
    11821 ttctttaagg gaactggcgg tttcaagtat catctgcgtg atctcaaaag atgcaggtaa
    11881 ttcgctgatt ctttctgcga caggaccaat ggcgacagaa aaattatttt tttgtccctt
    11941 ttctaattca tggaccaacg tttgggctac ctgataactg gtctcgatca cattttcttc
    12001 aagagaatca actattaaaa atttgatgaa tcgagaggaa acagcagaga agatcacttg
    12061 ttgattctcc ctaaataaaa acttcaaata atcactgaga tgagcgtaat cttcaaagtc
    12121 tgtactgact tggtttgctg ctaataatac ggtcattttt ttccctgcta aagagcgatt
    12181 gaaagattca gcaagtttca atgtttcact gatcgtaagt gagcctttga acaaaccgtt
    12241 caagaaatga ttttttttca attcgaaaac aagatcagaa gaagtgtgcg tttctttttg
    12301 ctgcaaatct aaagcagtta ctgctttttc tattgcttgt ttcaattcgc tacttttaat
    12361 cggtttaagc agataatcat ctgcctgtac ctggattgct cctttcatat aatcgaaatc
    12421 gtcgaatcca ctaataaata tgagacgagt ccaaggaagt agtttccttg cttcttttga
    12481 aaaagtcaac ccgtccatga aaggcatttg gatatcagtc agaatgatgt cgggctgtaa
    12541 gtcgataatt gaagacagtg ccatctcacc atctccagct tcgccgacat aagtgattgg
    12601 ataataagaa ccaaggacag ttatttgttc ccgcaagtta ttgcgaatga ggtattcgtc
    12661 ttcgatgata aaaactttat acatgtttgt ttcctccaat tgttttggga gatagaggga
    12721 tcatcagtgt gacagttgtt ccttctcggt atttgctgtc aatcgtgata ggtttttgat
    12781 catttccgta ataaagaagg agacgcttgt ttacgttgta aagtccgtag ccgatactga
    12841 aatctgaatc gatccctttg gctaactctt tacgtatttc tgccagtttt tctgcaggga
    12901 tgccaatccc gttgtctata actttgaatt ggatcatctc atcaattttg catgcggtaa
    12961 tacgaataaa tcctttgtga cggacgtact tcgttccatg gtatacagca ttttcaatca
    13021 atggctgcaa gatcattttt aaaatagaaa caggaaaaag tgtttctggt atatcgatct
    13081 tatagtcaag catatggccg tatcttattt ttaagatgac tagataatca ttgatatggc
    13141 taatttcttt ttcaagagga atccagtctt gtcctttgct taaagacgta cgaaaaaagc
    13201 cggaaagtgc atgggtcata gtcagagctt cttcaaagca gtcttgttcg atcaaagaaa
    13261 taatggcatc caaactgtta taaataaaat ggggagtaat ctgagcttgc aatacacgaa
    13321 cttcactttg tgctaaatgg tattgtttca aagcgttctc ccgtaaagac tggtttagat
    13381 cttccgccat tttattcatg ctagcggtaa gtgtttgtaa ttccagtgtt tccggcaggg
    13441 ttaaacgaaa gccaaggttc cctgaagaaa gttgttctga catctcgatc agctgattga
    13501 tcggttcttg gacctttctc tctaaaaagc gacgattctt tttcgcatag aaaaaaatgg
    13561 tactaagaac tccaagttcc actattgtta atagaatgag cgagcggaca agtgtcgtat
    13621 tttttttgct tgctaagtcg atctccacac gaacaaaatc ttgtaagata tctgacagca
    13681 gttgagtgac agaatctacc tgattgatga tctcttcatt ggtatccata aaatcttttg
    13741 ccaagatatt attgatcagc tcgttttgat aatcttccag tgtatcgatc gtacgtagcg
    13801 atacatctaa cgtactaatt tctttaagtg tatttgtatt atttttgatc tctcgaatat
    13861 cattcctaag ttcttctagg atattttcct cattgaactc atctacggtt acttggccag
    13921 aaatcaagtc ccacatactt tccaaaactt tttgatcaac cttcaaagca attgcatctg
    13981 ctttttggat atttgcgatc gtctgttgat aaatcaaaag ctgttgcgaa tataacagta
    14041 tgctcagtag caaagggata acagcaaata gaagcatggc tttgtttgct tgggcaaaaa
    14101 tctgttttat gcttaaattt tttgtcatga tacttctcca gtcaataatt tctggttggg
    14161 atgttagcta tattctgctg ggaaaagaca gtctcggaaa taaaaatatc tttaggtaat
    14221 gtttttcctg tttgaatatt tccaaaataa cgagtaacgg tattggctac ataccagccg
    14281 acatcaggtt tacactcgac tacacaattg acgattcctg agcgtagctt ctcgatcata
    14341 tcttcttgtc catcaatagt tacaatgatc aaatcttctc caggtttgat tgatgtttct
    14401 tttatcgcag ctaaagcacc gtgagtcatt tcatcactat ggctgtataa tacatcgatt
    14461 tcggataaat catttgtttc taaatatcgt ttcatctggt cttttccttt catccgaata
    14521 aaatcaccga caattcggtg aacaatagtc atcggaggtt tatggtcgat ggttccatct
    14581 ttttttaggg ttggtttacg attgattgat tccaaaaatc cttcggagcg tagcttggtc
    14641 ggtgtggagt tctctgaacc agcaagttct aggattctga tttcctgttt ctttgaattt
    14701 gtgaagtgat tgctgacaaa taaacccgcc cgatttcctt ctgctttgaa actggggcca
    14761 atgtgggtga cgaaaagctc gggatcattc gtttgaatat gtctgtcgac aagtatgaca
    14821 gggatccctg ctcgctttgc ttcttttaat acagagtccc acccggattc ttgcaaagga
    14881 gtaaaaatga tcatatccac tttataggca atgaacgtcc gtatatcttg gatttgccgt
    14941 tcctgattca tgtagccatt tcggtagaga acttggtaat cttctttttc aagcgcagtg
    15001 ataattgatt cagtatgctt tttccgccag ctactctccg ttcctgattg cgaaaaacca
    15061 acgactagtg ggtcaggtgc ttcctttgta ttaggtgtac accccatcat ttggtaagcg
    15121 attacaacaa gggctaaaaa aaataaaaca cgtttcactt taaatcactc catctggatt
    15181 tgttagtact atcttatcag ataaaaggga aaatcaaagt caaaaggaat caatcgaaaa
    15241 aaatccaaga aacttaaaaa aatccaagac aaaaaaagta cacctaaaaa atttaaagta
    15301 aatcccataa aaaaatcaat gttaacgctt tctttattag ctatactcgg tgtatcaatt
    15361 gaaatggggg ataaaaaatg aaaaacaaat ttagaaagat tgttgggact atcgctattt
    15421 tgaccgcaag cattctatta agtgcgtgtg gcaattcagg atctgcagat gatagtacgg
    15481 ggtatgtagg aatcgctatg ccgaccaaat cagcagaaag atggatcgct gatgggaaca
    15541 acatggtctc ggaattagaa aaactgggtt ataaaacaga tcttcaatat ggagaagata
    15601 aagtagaaaa tcaagtagca caaatagaaa atatgattac aaaaggggta gatacacttg
    15661 tgattgcttc tatcgatggt tcggctttaa ctgatgtgtt agctaaagca aaagaagcag
    15721 atatcaaggt gatcgcctat gatcgtttac tgatgaattc agaaaatgtc gattattacg
    15781 cgacttttga taattttgga gtaggtgtat cgcaagcagc ttatattgaa gagcacttag
    15841 gattgaaaga aggaaaagga ccattcacga ttgaattatt tggtggctcg cctgacgaca
    15901 acaacgcact aatcaattac aacggagtga tgtctgtttt acaaccatat atggacaacg
    15961 gtcagttggt cgtgccatct ggacagacga gtttcagtca aatcgctacg cttcgctggg
    16021 atggttctac ggcgcaagca aggatggata atctgttaag cgcaaattat actgaccaaa
    16081 cattggatgc tgttttgtcg ccatatgatc caataagttt aggaatcatt tcgtcattaa
    16141 aaggcgtggg gtatgggagt gaaagtaaac ctttacctgt gattactgga caagatgcga
    16201 cagttgctgg cgttaaatcg atcattgccg gggaacaaac acaaacgatc tttaaagaca
    16261 cacgtatctt agcaaaaaat acgattgaaa tgatcaaagc aatcagtgat ggagaagagg
    16321 ttccggtcaa tgatactgaa acttatgata atggggtgaa aactgtccct acgtatttag
    16381 caaatacagt atctgtcgat aaagataatt atcaagcaga gctgatcgac accgattatt
    16441 ataaagaatc cgatctgaaa aattaagagg aaccaaacaa ggaactaaag agatctaagt
    16501 cagagagagg ataagaatta tggcggattg tattttaaaa atggcagata tcaccaaaaa
    16561 attttctgga atccgagcgt tagattgcgt gaatctgatg attgaacgtg gggaaatcca
    16621 tgcattgtgc ggagaaaacg gagcgggaaa atcaacattg atgaatgtct tgagcggctt
    16681 gtatccatat ggctcctatg acggggaaat cttttatgat ggaaagctgt gtcaatttaa
    16741 agatttgaaa gatagtgaag aaaaaggcat cgtcatcatt catcaagaat tagcactaag
    16801 tccttatctt tcaataaaag agaatatttt tctcggaaat gaacaagcag aaagaggcgt
    16861 gattgattgg gaactgacag aaaagaaaac atatgaactg ctgaataaag tcggtttgtc
    16921 tatcgacccg aatcttctag tatcccagat tggcgtaggg caacagcaac tagttgaaat
    16981 agctaaagca ttatcaaaat ccgtccgtct gttgattttg gatgagccta ccgctgcatt
    17041 gaatgaagaa gaaagcgcga atttgctgag attgatccga gagttgaaag agcaaggtgt
    17101 aacctccatc attatttcac ataagctgaa tgaaatcgtg gatatcgcag atcgaatcac
    17161 cattttacgg gatggacaga cgattgagac actagaaaat gaaatgatca gcgaagagca
    17221 tattattcgc gggatggtgg gcagagattt agttgatcgt tatcctgaaa gaaaaccgga
    17281 tattggagaa gtttatttta aagtagaaga ctggacggtc tatcaccctc ttgatcacga
    17341 gcgaatcata aacgaccaca tcaacttgca tttgaaaaaa ggagagattg taggaatagc
    17401 tggactgatg ggagcgggca gaactgaatt cgctatgagt ctatttggac attcttatgg
    17461 aagcaagata tccggacgta tattaaagga aggggcagag atatcagtta agaccgttcc
    17521 ttcagccatt gcaaacggac tcgcttatgt ctcggaggac cgaaaatctt taggactgaa
    17581 tctgctgatg gatattcggg aaaacgtgac gctgagcagt ttggataaaa taagtcaaaa
    17641 aggagtatta gataaagaaa aagaagtaac tgaggcagaa aatttccgga aaaagatgcg
    17701 tataaaaaca aactccatcc atcaaattgt cagtagtctt agtggtggga atcaacaaaa
    17761 agtcgtttta tccaagtggc tgatgacaca acccgatatt ctttttttgg atgagccaac
    17821 ccgtgggata gatgttgggg caaaatatga gatctacacg attatcgaag aaatggcagc
    17881 aaatggcaaa gctgtctgtc ttatctcttc tgaacttcct gaaatattag ggatgtgtga
    17941 tcgaatctat acgatgaacg aaggacgtat tacaggagaa gtctcgcgag aagatgcaga
    18001 tcaggaagtc ttgatgaaat taatgacgaa agaagaggtg ggttaagttg gaacaggcaa
    18061 aaaatcttac ggataaaaaa gagaaaaaac taaatttgaa aagtagagca gctgatcttt
    18121 ttggtcgcta cagcatggtg atcattttag cgggactact tattgcgttt caattgatga
    18181 cggatggtat tttctggcgt ccattgaata taactaacct tgttctgcaa aatagccata
    18241 tattagtact agctgcaggg atgttattag ttgtgctctt aggtcatgtg gatttgtcgg
    18301 tagggtcagt gatggcgttt gttggtgcaa ttgcgggcat gatgatggtc aatcgccaaa
    18361 tgaacccgtg gatgacagtt atcgcatgtc ttgcagtagg cgggctgatc ggcgcatggc
    18421 aaggtttttg ggtagcttat gcagggatac cggcgtttat cgtgacttta gcaggattgc
    18481 tgatgtttcg tggactgaca caagttgttc taggaggcca atctctagca ccttttccag
    18541 ctgttttcca aaaaatgtct acgggttatt taccagactt aagcgaagga agcagccttc
    18601 acttcttgac attgatttta ggaatgatca tcacgatcgt gttagttgtt ggtcaatggc
    18661 gaacaagaaa tcgtcggaaa aatcatttat ttgaagtgga atctccaaca gctttcttgg
    18721 taaaggcggt atttacagca gtcgttgtac taggaataac ttatatcttt gcctcttatc
    18781 aagggtatcc gattattttg atcattcttg gtttgatcgt tgcagcatat gggtttttga
    18841 caaataaaac agtagcaggt cgacagattt atgcgacggg cggaaatcga aaagcggctg
    18901 aactatcagg gatcaaaaca aagcgaatca cgttctgggt attcgtcaac atgggagtca
    18961 tggcagcttt agctggatta gttttagctg ctcgattgaa tgcggcaaca cctcaagcag
    19021 gtacatcgat ggaactggat gcaatggcgg cagtatattt tggtggtgct tcgacttccg
    19081 gcggcattgg aacaatcatg ggtgcgattg tcggtggctt ggtcatgggt gtattgaaca
    19141 atggcatgtc gattttaggc gtcggggtag actggcagca ggcaatcaaa ggattgatct
    19201 tgcttttagc cgttgtgcta gacttgtata ataagaaaaa acgaacaacg taggagaacg
    19261 ccggttattc agaaaaaaga ctgtgacaag acatttgtca cagtcttttt ttcgctaaaa
    19321 gcgaacattc attgtttcgt ttgtatagac taaaaataat atatgttttt ttaccattcg
    19381 tataaattct ctttttgctc ttgatttttt tgcttaattg agttaaatta catagggcgc
    19441 taaaaaaagc cttgattaaa ttaataatgt tcgtgttttg ctggatgtga acatcaggag
    19501 gataatgtaa tgaaagtatt tgattacgaa gatattcaat taattcctaa taaatgtatc
    19561 gtaaatagcc gttctgaatg tgatacaacg gtcacattag gtaaacatac attcaaaatg
    19621 ccagtagtgc ctgccaacat gcagacgatt attgatgaaa caatcgcaga attcttagcg
    19681 gaaaatggtt atttctatat catgcatcgg tttgatgaag cagctcgaat tccgtttatc
    19741 aaaaaaatga aaaagcgtgg attgattacc tcgatcagtg ttggcgtcaa gaaagaggaa
    19801 tattccttta tagagaagtt ggcagaagaa tcgctaaatc ctgattatat cactatcgac
    19861 atcgcgcatg gacatgctaa ctcagttatt gatatgattc agcatattaa aaaatatttg
    19921 cctgaaactt ttgtgattgc cggtaatgtt ggtacaccgg aagccgtccg tgaattggaa
    19981 aatgcgggcg cagatgcaac aaaagtcggg attggtccag gaaaagtatg tattacaaaa
    20041 atcaagacag gctttgggac tggaggctgg caactggcag ctcttagatg gtgtgcgaaa
    20101 gcagctcgca agccgatcat cgctgacggt gggatcagaa cacatggcga tattgcaaaa
    20161 tctgtccgtt ttggtgcgac aatggtcatg attggttcat tgtttgccgg tcacgaggaa
    20221 tctcctggcg aaacaaaagt cgaaaatgga atcgtgtata aagaatactt tggttctgct
    20281 tcagaattcc aaaaaggcga aaaacgaaat gttgaaggaa agaaaatctg gatccaacac
    20341 aaaggatctt tgaaagatac actagtggaa atgcaacaag accttcaatc atctatttct
    20401 tatgctggag gaagagatct ggaagccatt cgaaaagttg actatgtcat cgtgaaaaat
    20461 tcgattttta atggagacac aatctaaaaa ataaataaaa tattaggatg ttgtgaaaaa
    20521 gttgtcctgc attaagtaat aagaacagcc aaacaaaaat agttctttat atgttttgtt
    20581 tggctggaat tattatcgca gatacaagct tttgaacatc attattcaga ataagggact
    20641 gtgataagat ttttgtcaca gtcccttatg ttttgcaaaa aaagtttttc ttttttctcg
    20701 taaattcggt aaaatcaaaa gaaaaaatag acaatagaaa agagaagctt atgtgggaaa
    20761 aagtagagaa aaaacaagga ccgatttatc gtcaaattat ggaacagatt atgagagaaa
    20821 tcgaaaacgg aaggatcaat cctggaagtc aacttgaatc agaacgaaaa ctagggcaac
    20881 tttttggtgt gaatcgttcc accgttgtcc atgcattgga agagcttagg gagcttgggg
    20941 tactaacgag caaacgtggc agcggatggt ttgttaatca gactgaatgg gggaaattcg
    21001 ctgttccaag aatcgattgg cggcaaatga tctctcctcg ttatgagcag acggactggt
    21061 atgagcaaaa aataagagaa gcaaagcaga tccaaaaaac atcgtttctt gatttatacg
    21121 ctagcgagat gccagatgag cttttgccga attttgagtt tccttcttat tctcttgaag
    21181 aaatcttagc ggaagaaaaa gaaatggatc ttttaggcta tactccgtta cgtaacaaaa
    21241 tcaaacggta tttggaaagt aaatttgaat tggcattttc ccccaaccag cttcttgtga
    21301 ctggtggcgg tcagcaagca atctttttga ttttacaaac gattctttca gctggagagg
    21361 ggatagctgt agagtcacct tctttttttt accgattacc tcttttcaaa gcttcaggaa
    21421 cgcgtttatt tgggataccg atggatgaag aagggatcga cttagaaaaa ctgaaagaat
    21481 cgatccataa aaataaattg aaagcagtgc ttatcaaccc gaattttcaa aaccctacag
    21541 gaaaagtgat gagtcaaaaa cgacgagaaa agctagtaga attgtgtcga cagtatcggc
    21601 ttccaattat tgaagacgat gtatttacgg atctttcatt tctaaaccaa gaagaagtcc
    21661 ttcctatacg aacgattgat ccggataatg tgttatacgt cggttcgtta tctagggttt
    21721 taggaaaaac aacgaaaatc ggttgggtga taggacctga gacttttatc tcgcaactag
    21781 ctcaagcgca agaaatgatg gagttttcga tgaatatcct tacacaaatc acagtggcta
    21841 atgtatttaa tgaagcaatt gatttgaaaa tgcaagatgt aagaaaaaag ttgaaagaaa
    21901 aaagccgact gataagcgct tggggagatt cacaatcgtt tttcaagctt cattcaattt
    21961 taggtggtta ctatgcctgg attacttggg atgggaaaaa actagacccc gaactagcag
    22021 aaaaagtagt agatatgggt cttggaattg caccaagtct actttttggg caagatataa
    22081 atggaatcag aatcaattac agtaggctgg atgaaaacca aataccgttg ttttattcaa
    22141 agatgaaaat tctagaaaag tggttaacag aataatttca caaagtaaat agattaaaga
    22201 atataacagc ttggatttaa atttcaagct gtttcttttt tttcggaaaa agaatacgat
    22261 ataaagaaac taagaaaaaa ccattatttg tacgtccgtt cacgatgtgt aaataaaata
    22321 aatgtttgca acaaaactgt ataacaccaa cgtttttaaa tagaaacaga tggttgtaag
    22381 tttgattaga aaatagaaaa aatgcagatg aaaaataaaa cgctttcctt attgtgattt
    22441 tattcactaa tgctgttaat atgcggattg gcataatttc ctttcatcgt gaaaatttgt
    22501 gcttttagct tgtatttctc ataaatccat gataatttat taatgaagac ggttaaatag
    22561 tgatacagtt ttattcactg tttcaatcgc agaatgaaga gattttctta tagatgagag
    22621 aggaatgtgt gacaaatggc aaacaaaaaa gcaccgatcg acttccaagc actgctagat
    22681 gaagtcaatg cagatttccc agtatatcaa gcacttgatc aagatggaaa cgtagttaac
    22741 aaagatttag tacctgattt atccgatgat gaactcgttg aattaatgac tcatatggta
    22801 tggtctcgtg tattagacca acgttcaaca gcgctgaacc gccaaggacg cctaggcttt
    22861 tttgcaccaa ctgctggaca agaggcaagt cagctggcta gtgcttacgc atttgataaa
    22921 gaagatgttc ttcttccagg ttatcgggat gtacctcaac tagtaaaaca tggtttacct
    22981 ttatctcaag cttttttatg gtctcgagga catgctgcag gtaattctta tccggaagaa
    23041 ttgaaagctc taccaccaca aattatcatc ggtgcacaat atgtacaagc agccggtgtt
    23101 gcattaggtt tgaagaaacg aaacaagaaa aatgtcgtct tcacttatac tggtgatggc
    23161 ggttcatcac aaggtgattt ttacgaagca atcaatttcg ctggcgctta ccatgcaaat
    23221 gccgtatttt atatccaaaa caacgggtat gcgatctcta cacctcgtga aaaacaaact
    23281 gcagcaaaaa cacttgcaca aaaagctgtc gcagctggga ttcctggtat tcaagtcgat
    23341 ggaatggatc cgcttgcagt atatactgta tctaaaatgg cacgggaatg ggcgatcagt
    23401 ggaaatggac cagtcctaat cgaaacactg acttatcgtt atggaccaca tacactatct
    23461 ggtgacgatc caacccgcta ccgtacaaaa gacacagatg acgattggca gaaaaaagat
    23521 ccattgatcc gcttccgtaa atatttgacg gaaaaaggac tttggtctga agaaaaagaa
    23581 gaacaagtca tcgaagcaac taaagaagaa atcaaagcag ctattgctga agccgacaaa
    23641 gtaccaaaac aaaaggtttc agatttcttg aaaaacatgt tcgaagtatc tccacaatca
    23701 atcaaagaac aaattgcaat ttatgaagcg aaggagtcga aataatcatg gcacaaaaaa
    23761 caatgatcca agcaatcaca gatgccttag cacttgaact agaaaatgac gagaatgtcg
    23821 ttgtttttgg tgaagatgtt gggaaaaacg gaggagtttt ccgggcaact gaaggattgc
    23881 aggaaaagtt cggagaagac cgagtattcg atactccttt agctgaatct ggaatcgcag
    23941 gattgagttt cggtcttgcg ttagaaggat ttcgtccagt tcctgaaatc caattctttg
    24001 gttttatttt cgaagcaatg gacgaagtag tagctcaaat ggctcgtaca agatatcgaa
    24061 tgagcggcac tagaaatcta cccatcacaa ttcgttcgcc atttggtggc ggtgttcata
    24121 caccagaatt gcattcggat aatttagaag gtttgatcgc tcagtctcct ggtattcgag
    24181 tagtgattcc ttccaaccct tatgatgcaa aaggactatt gatttcttct attagaagta
    24241 acgatccggt tgttttccta gaacacatga aactatatcg ctcattccga gaagaagtgc
    24301 cggatgaagc ttacgaagtt cctttggata aagcagcagt tactcgtgag ggaacagatg
    24361 tatctattat cacatacgga gcaatggtgc gtgaagcaat caaagcagca gacaatcttg
    24421 caaaagaaaa tatatctgtt gaaatcattg atcttcgtac agttgcgcca ttagatgtag
    24481 aaacgatcat tcaatccgtg gaaaaaactg gtcgcgtagt tgttgtccaa gaagcacaac
    24541 gacaagcggg cgttgccgcg caagtagttt ctgagatctc agaacgcgct atcctttcat
    24601 tagaagcccc aatcggacga gtttctgcac ctgatacagt cttcccattt ggacaagcag
    24661 aaaatgtctg gttgccaaat gcaaaagata tcgaagacaa agtaaaagaa atcgcggaat
    24721 tttaagaaaa gaagggacga aacaaagtca tggcttatca atttaaattg ccggatatcg
    24781 gtgaaggaat cgcagaaggt gaaatcgtca aatggttcgt caaaccagga gatacgatca
    24841 atgaagatga tacattacta gaagttcaaa atgacaaatc agtagaagaa atcccgtcac
    24901 ctgttacagg aacggttaaa aatgtgattg ttccagaagg taccgtagca aatgttgggg
    24961 atgtattagt agaaatcgat gcaccaggac acgaagacaa tgaaggggac agtggtgtag
    25021 ccgcagaatc ccaaactcca gctaaaccgg ctgctgaacc cactgttgat acagaatcag
    25081 ctggatcgtc aagcgaagga gtcttccaat ttaagttacc ggatatcggt gaaggaatcg
    25141 cagaaggtga aatcgtcaaa tggttcgtca aaccaggaga tacgatcaat gaagatgata
    25201 cattactaga agttcaaaat gacaaatcag tagaagaaat tccgtcacct gtcacaggga
    25261 cagttaaaaa cgtgattgtt ccagaaggca ctgtagcaaa tgttggggat gtattagtag
    25321 aaatcgatgc tcctggacac aatagtgcac cgtcaactag tgcgccatca gctgaagcac
    25381 caaaagaaaa agtggaaaca tcaggttcag caagtgtcgt tgaagctgcc gatccaaata
    25441 aacgtgtatt ggctatgcca tctgttcgtc aatttgcccg tgaaaaagat gtagatatct
    25501 cacaagtaac tgcaactgga aaaggtggca gagtaacaaa agaggacatc gaaaacttct
    25561 tggcaggtgg tccaagcagt gcaccagcga aatcagaagc gccagaagcg gcagcaccga
    25621 aagaagcagc tcctgcagca gaaagcaaac cagctgcacc agcaaaacca ttcaagtcca
    25681 acttaggaga cttggaagaa cgtgtagcaa tgacgccaac acgtaaagct atcgctaaag
    25741 caatggtcaa cagtaagcat acagctccac acgtgacatt gcatgatgaa gtagaagtgt
    25801 ctaaactatg ggataatcgg aaacgattta aagaagttgc tgctgcaaat ggtacgaaac
    25861 ttactttctt gccatatgtc gtgaaagctt taacagcgac tgttaagaaa tacccagtat
    25921 tgaatgcatc tatcgacgat gcaaaccaag aaatcgttta taaacattac tacaatatcg
    25981 gtattgcaac tgatacggat cacggactat atgtaccaaa tgtgaaagat gcagaccgca
    26041 aaggaatgtt tgctattgca gacgaaatca atgagaaagc aaaattagct catgacggca
    26101 aactatctgc agaagatatg cgcaatggaa caattacgat cagcaatatc ggatctgtag
    26161 gtggcggttg gtttacacca gtcatcaact atccagaagt agctatttta ggtgtaggaa
    26221 cgattgcaca acaaccaatc gtaaatgctg aaggcgaaat cgttgtcggt cgtgtgatga
    26281 aactgtcatt aagtttcgat caccgtatcg tagatggagc aactgcgcaa caagcaatga
    26341 ataatatcaa acgtctttta gctgatccag agctattaat gatggaagga tgataaacaa
    26401 atggtagttg gagattttgc tgtagaatta gatacagttg taattggtgc gggacctggt
    26461 ggctatgtag ctgccatccg cgctgctgaa atgggacaaa aagtagcaat cattgaaaga
    26521 gagtatattg gcggtgtttg tttgaatgtc ggctgtattc cttcaaaagc actgatagct
    26581 gcaggacacc attatcaaga gtcactagat tctgaaatgt ttggagtaac tagtgaaaac
    26641 gtcaaactag attttgcaaa aacacaagaa tggaaagaaa acaaagttgt ccatacgttg
    26701 acttctggtg ttggtttcct attaaaaaaa cataaggttg aaacaatcga aggagaagcg
    26761 ttcttcgtag atgatcacac actacgcgtc attcatccag attcagctca aacttattcg
    26821 ttcaataacg caatcatcgc aacaggttcc cgtccaatcg agattccagg atttaaattc
    26881 ggaggacgtg tgttagattc tactggcgga ttagcattga aagaagtacc taaaaaattt
    26941 gtgatcatcg gtggcggtgt gattggtgcc gaattgggcg gagcgtacgc aaacttagga
    27001 gcagaagtaa cgattcttga aggctcacca caaattttac caacttacga aaaagatcta
    27061 gtaaaattag tagaagatga tttcaagaaa aaaggcgtaa cagtcgtaac gaatgcaatg
    27121 gcgaaagaag cagtcgataa cggtgacagc gtgacagtga aatatgcagt ggacgggaaa
    27181 gaagaatccg ttactgctga ttatgtgatg gtcactgtag gtcgtcgtcc gaatacagat
    27241 gatatgggct tagaacaagc gggtgtcgaa gtaggagaac gaggattgat cacggtggat
    27301 aaacaaggac gtacgaacgt acctaacatc tatgccatcg gtgatatcgt tccaggggcg
    27361 gcattagcac acaaagccag ttatgaagca aaaatcgctg ctgaagcaat ctctggcaaa
    27421 aaagtagctg tagattataa agcaatgccg gcagtagcct tcactgatcc tgaattggct
    27481 tctgtaggta tgacgatcaa agaagcaaaa gatgctggta tcgaagcaaa agcatataaa
    27541 ttcccatttt cagggaacgg tcgggcatta tcgcttggta aaacagaagg attcatccgt
    27601 ctagttacga cgatcgaaga taacgtgctt attggcgcgc aaatcggtgg agtcggcgca
    27661 agtgatatgg tctctgaatt agctttagca atcgaatcag gaatgaatgc tgaagatatc
    27721 gccttgacga ttcatcctca tccatctctt ggcgaaatcg tcatggatgc ttcggaatta
    27781 gcattaggtt taccaatcca tatttgattt gaataataca ggcgaaacgc aactgaaaaa
    27841 tcagttgcgt tttgtttttt agaattatca ataatattat tatgtaaact taaaaatatg
    27901 acaacttctt ggtttattac cacttcctat gctacactga agaggaatat gaaataggag
    27961 agttgaagat gcaagaatat cagtatccac ttgatttaga ttggacaaca gacgaaatgg
    28021 tcattgtaat gaagatgtgg gaagatctgg aacgtgcgaa tgaaaaaggg ataaacaatg
    28081 aagctttttt gaagacttat cagcagttta aaactgttgt caaatccatt ggtgaggaaa
    28141 aacgattggg acgggaattt gaaaaagctt ccggttattc tttatatcgt acagtgcagc
    28201 aagcaaaaaa aaatccaaac aaaactttga gaatgaacgg gtgagataaa tggaacagat
    28261 gattgatcat atcaagtcct ggcttgaaga agctggtgag aaaatcaaat cttctcaaca
    28321 aacattaaca atctcgcaaa aatcaaatcg aacggatcta gtaacaaatg tcgatcgaaa
    28381 catccagaat tttctaatcg aaaaaatcca ttcctttgat ccagaggcga aaatattggc
    28441 agaagaagaa gggtgtaatc aattatcgga tctcgatgga agagtcttta tcatcgatcc
    28501 tattgatggc accttgaatt ttgtgattga aggggaaaat ttttgtatta tgctcgctgt
    28561 ttatgaatcc ggcgttggaa agcttggttt catttatgac gtgatgagaa atgaactttt
    28621 ttggggtgga aaaggaatag gcgtgtataa aaatgatgag ccattgccta gaccagcaaa
    28681 taaatcctta tcggaaggct tacttggtgt gaatagttat cttttcggtc ataataaata
    28741 tggaatccga gaaattgcgg aaagcagcat ggggacacgt atgtacggat gcgctggtct
    28801 tgaactagtg gcaatcttga aaggaaacca cattgcttat atttcaaacc ttagcccttg
    28861 ggattatgca gcgggaaatg tcttattgga agagtttggt atggtaacaa gcggtctttt
    28921 tggtactcct ttgacatttg aaaaaaggga atactattta gcagggactt cttgtgcata
    28981 tgcagaaatc gcagaccttt tgaaaaaaga aggctgaaaa aaaggtagaa atcattcaca
    29041 aaaatgataa aaacgagcaa aagattaatt ttctcgaaaa aatgaaaaca ttttactata
    29101 tttcatgaaa aaagtctgct ataattagca gtcgagtaaa atttgccctg tcataaagaa
    29161 ccaataaaaa aggagttagt tacattgaat tatagaaacg atatccgtaa cgtcgcaatt
    29221 attgcccacg ttgaccacgg aaaaacaaca ttagttgatg aactattaaa acaatcagat
    29281 acattagacg cacatacgca attgcaagaa cgtgcgatgg actcaaatgc gctagaaaaa
    29341 gaacgtggta tcacgatttt agccaaaaac acagctgtag actataaagg tattcgcgta
    29401 aacatcatgg atactccagg acacgcggac ttcggtggcg aagtagaacg gatcatgaaa
    29461 atggttgacg gcgttgtgtt ggtcgtagat gcgtatgaag gaacaatgcc tcaaacacgt
    29521 tttgtattga aaaaagcact ggaacaacat atcacaccaa tcgttgtcgt aaacaagatt
    29581 gacaaacctt ctgctcgtcc tgagcatgtc gtagatgaag ttcttgaact attcatcgaa
    29641 ttaggagcag atgatgatca gttagacttt cctgtgattt atgcatctgc actaaatggt
    29701 acgtcaagct tgtcagatga tccagcagat caagaaccaa ccatggcacc tatttttgat
    29761 acaattattg aaaaaatccc tgctcctgtc gataacagcg acgaaccatt acaattccaa
    29821 gtttcattac ttgactacaa cgattatgta ggtcgtatcg gaatcggacg tgtatttaga
    29881 ggaacaatca aagtaggaga ccaagtagca ttaattaaat tagacggaac agttaagaaa
    29941 ttccgtgtaa cgaaattatt tggtttcttt ggactaaaac gtctagagat tcaagaagca
    30001 aaagctggag acttgattgc tgtctcaggt atggaagata tctttgtcgg tgaaacagtt
    30061 actcctgtgg atcatcaaga tgcgttacct atccttcaca ttgacgaacc aacattgcaa
    30121 atgactttct tagttaataa ttcaccattt gccggtcgag aaggtaaatt cgtaaccgct
    30181 cgtaagattg aagaacgttt gatggcagaa cttcaaacag atgtatcctt acgagttgaa
    30241 ccaacaaact ctccagatgc atggacagtt tctggtcgtg gagaattgca tttgtctatc
    30301 ttgattgaaa acatgcgccg tgagggatac gaattgcaag tatctcgtcc agaagttatt
    30361 gaaaaagaaa tcgacggtgt aaaatgcgaa ccgtttgaac gcgtgcaaat cgatacacca
    30421 gaagaatata tgggtagtgt tatcgagtcc ttgagcttac gtaaaggtga aatgcaagat
    30481 atggtccata caggaaacgg acaaatccgt ttgacattct taacacctgc ccgcggtttg
    30541 attggttatt caactgaatt cctttcaatg acacgtggtt acggaatcat gaaccatact
    30601 tttgaccaat atctgccaat gcttcctggt caaattggcg gacgtcacca aggggcgctt
    30661 gtttcgatcg atacaggaaa agcaacgact tattctatca tgagtatcga agaacgcggt
    30721 acagtcttcg tagaaccagg tacagaagta tatgaaggaa tgattatcgg ggaaaacagc
    30781 cgtgataatg atttgactgt caacatcacg aaagcaaaac aaatgacaaa cgtacggtct
    30841 gctactaaag accaaacatc tgtcatcaaa aaaccaaaac aattgacgtt ggaagaatct
    30901 ttggaattct tgaatgatga tgaatattgc gaagtaacac cagaaagcat tcgcttgaga
    30961 aaacaaattt tagagaaaaa tgctcgtgaa aaagcatcta aaaagaaaaa ataatctgta
    31021 aaaggatgaa gagagaaacc agcttgttat aaaaaatagc tggtttcttt ttttgtccaa
    31081 ctcttttctg tggagaagtc atctttgtca atggacataa attcagaaaa tacgccgaaa
    31141 gacgtttaaa agacttttaa aaaacttatt tctagagagt ttttgtttca taataagata
    31201 atgtttcacg aaaggagatc aacttgtcct tcgaagaaaa tcgaagtaaa atggcaagag
    31261 aaaggagcgg gaagatgaac gacttattaa aacaatactt tggttatgat gagtttagac
    31321 ctggtcaaaa agaaattatc cagaaagtca ttgatcagga aaatgtatta ggaatcatgc
    31381 ctaccggaag tgggaaatca atctgttacc agcttccagc tttactatta gatgggctta
    31441 ctgtggttgt gtccccttta atctctttga tgaaggatca ggtggacgcg gcgaatcaat
    31501 taggtatacc cgccactttt atcaatagct cgttagatgg ttacgaaaca gctagacgct
    31561 tccaagaaat cgatagacag cagtaccgtt tactttatat cgctcctgaa cgttttatca
    31621 tgccggattt tatccaagca atgaagcgat ggaacgtccg tatgatcgct attgacgagg
    31681 cacattgtat ctctcagtgg gggcatgatt tccgcccaag ttatttacag atggcgaacc
    31741 agctagatca actgccaaat cgtcctgtaa ttgttgcttt gacagctaca gcgactgtgc
    31801 aagtagctgc tgacataaaa agacttttaa aaattccaga aaacaaccat attcagactg
    31861 gttttgaacg agaaaatcta cgatttcaag taattaaaga tcaaaaaaaa gaacaatatc
    31921 taatcgaata tttaaagatc aacaaaaatc aatctggtat catttatgca gcaacgagaa
    31981 aagaagtaga ccgtctctat catttgctta aaaaattcga tttttccgtg ggtagatatc
    32041 atggtggtct aaatgagaat gagcgaacag aaatgcaaga agccttttta tacgatcgct
    32101 tgcagctgat tgtagcgaca aatgcatttg gtatgggtat aaataaaagc aatgtccgtt
    32161 ttgttatcca ttatcaaatt cctggctctc tagaagcata ctatcaggaa gctggtcggg
    32221 cgggaagaga tggtctttcc agtgaagcca tcctgctttt ctctcctcaa gatatccaag
    32281 tccaaaagtt ttttatccag cagtcccaaa gagaagaagg gcaaaagcaa aaagaatatg
    32341 aaaaattaaa agcgatgaca gaatacgtgt atattgagtc ctgtcttcag caatacatcc
    32401 taaactattt tggcgagaca agcagtccgt gcaaccgttg tggtaactgt ttagatgaca
    32461 gagaaatagt agaagtaacg acagaagcac agatggttct ttcttgctta aaacgtatgg
    32521 gtgagaacta tggtaaacaa atgctgatga aagtcttagc aggatcgaaa gaacaaaagc
    32581 tgcaagcttt aggttttgga catttatcta cctatggact gatgaaaaac caatcgcaaa
    32641 aagaaacgat gcagttaatt gaatatttga tatcgaatgg ctatcttctg accatcaatg
    32701 gtgagtatcc tgttttgaaa gtaacagaaa gagggataca agtattgaaa ggacaagaat
    32761 ctgtttatcg aaaggaaccc aagaaagtcc agcagctatc ggatgaagaa accgatacac
    32821 tatttgaagt gctgcgggaa ctgcgaacgg atctagcatc agaagcaggc gttcccccgt
    32881 atgtcgtatt ttcagattcc acattgaaag aaatgagccg aatacgtcca tcaagcagat
    32941 tggagatgct tcagataaaa ggagttggcc aaagcaagct tgataaatat ggcgaagcct
    33001 ttttatctcg gataaaaaat gcggactcta acgttctgga taagtagaaa gaacgaatac
    33061 gtaaaaatga ataaaaacaa ctggttaaat ctgttttttt tgctccttat gaaaatcaaa
    33121 tatgttataa ttaaggaatt agaatgggag gtaagcacag atggattctt tgtcgaaaaa
    33181 acttgcgatc caagtattaa atgatgaagc agaacggatc aaacgattaa tccgaaatca
    33241 aaacaacagt ctctgtatct cacaatgcaa agccttcgaa gaagtggtag atacacagat
    33301 gtacggcttt tcccggcaag tcggttttgc tgtccgtata ggagtaatcg gaaagaacga
    33361 gggccagctg atgttaagtg agctagaacg agaattgaat cagttatata gtgaggttta
    33421 ccaagaaatt tatgataaaa aagaagttgg caaggaggaa tagacttgcc taagaaagtg
    33481 aaaaaaaggc atctattaga ctatagcatc ttgattccct atttaatttt atgtgtattg
    33541 ggattgatca tggtatatag ttctacgtcg tatctattat tagaaaacgg acaaaatccg
    33601 tccgcatcag tcattaacca gagtattttt tgggtgctta gcttgattgc tatcgcacta
    33661 ctttataaaa tgaaaacaga tgttttgaaa aaccagcgtt tgatcatggc agccatcgca
    33721 gttttgacga ttttgctgct gattgttgtg ttttttggta aggagatcaa tggggcgaaa
    33781 ggttggctgc aaattgcagg attctcgatc caacctgccg aatacctaaa aatcatttct
    33841 atctggtacc tgtctctgac tttatctaaa agacaaaaca gtgtacaaaa agattttctt
    33901 gggactgtga aacgtccact tgcaatggtt atcggactta ctgcattagt cgctattctt
    33961 cccgattttg gtaatgcggc agttatcttt ttgattattt tggttctgct tttagccagc
    34021 ggagtcaatt atgtgtatac tttgattgta ggagtaggag gcttttgtct aagtactttt
    34081 acgatctggc tgatcaatat cacaaacgga aagatcttac ccggacgttt acagtatatc
    34141 tataatcgat ttgctattta ccaaaatcct ttctcagacg aattaaataa agggcatcaa
    34201 ctagtcaacg gctactatgc catgttcaac ggaggactat ttggcagggg attaggtaac
    34261 agtatccaga aaaaaggatt tttacaagaa gcacaaaccg attttattta tgcaatcgta
    34321 gtggaagaac tgggagtgat tatggggatt ttgatattag cgttgctatt ctttatgatt
    34381 gtgcgcattg tccttgtagg tatccgttca aaagatccgt tcaattcgct tttgtgcatt
    34441 gggatcggcg caatgttttt gatccaagta tttgtcaatc ttggaggaat tactggcgtt
    34501 attcctttga cagggatcac gttcccattt ttaagtcaag gaggctccag tttgctgatg
    34561 ctttcgattt gtgtcggctt tgtcctcaac atcagtgcgg atgaaaaacg caagagctta
    34621 ggtttataat aaaaaaagat gtaggagtgg ttcgatgaaa aaagttttag ttgctaacag
    34681 gggtgaaatc gctgtacgtg tatttcgtgc ttgtacagaa ttaggaatta aaactgttgg
    34741 aatctatgca gaagaagatg aatattccgt acaccgtttc aaagcggatg aagcatattt
    34801 agtaggacaa ggaaaaaaac cgattgatgc gtatctagat attgaaggga tcatttctat
    34861 cgcgaaagaa tgcggagctg acgctattca tccgggttac ggtcttttgt cggaaaactt
    34921 gaattttgcg caacggtgcg aagaagaagg catcattttt gttggaccca aactgcatca
    34981 tttagatatt tttggagata aaatcaaagc aaaagctgca gctatcgaag cggggattgc
    35041 ttcgattcca ggcacagatg gaccaatcgc ttctatagac gatgccttgg agtttgcgaa
    35101 acaatacggt tatccgatta tgatcaaggc agctctaggc ggcggcggac gcggcatgcg
    35161 tgtagctcac gatgaaaaga gtgcaagaga gggctacgaa cgtgcgaaaa gcgaagcaaa
    35221 agcagccttt ggtagtgatg aagtgtatgt tgaaaaatac atcgctaatc ctaagcatat
    35281 cgaagtacag attctcgggg atacgcatgg aaacgtcatc catctttttg aaagagattg
    35341 ttctgttcag cgccgtcacc aaaaagtagt agaagtagcg ccttgtgtct ctatgaatga
    35401 acagcaacgt caaaaaatct gccaggcagc tgtccaattg atgaaatatg tcgggtatgt
    35461 caacgcaggt acagtagaat ttcttgttga aggagatgat ttttacttta tcgaagtcaa
    35521 ccctcgcgtc caagtggaac atacgatcac agaaatgatt acagatattg atatcgtaac
    35581 aacgcaactg ttgattgctc aaggtttgga tctgcataaa gaaatcggat tgcctcagca
    35641 agaaggaatt aaattaaatg gttcagccat tcaatgccga atcactacag aagacccgtt
    35701 gaataacttt ttaccagaca cagggaaaat cgatacgtat cgttcacctg gtggttttgg
    35761 cgtacgtctg gatgtcggaa atgcttacgc gggctatgta gtgacaccgt attttgattc
    35821 attgctagta aaagtatgta cgcatggtgc tacctttgaa acagccattc agaaaatgga
    35881 acgttgcttg aaagaattta gaatccgtgg agtgaaaaca aacattccat ttatgctcaa
    35941 tgtcatcaca catcctgaat tccaatcagg aaatgcaaaa acaaccttca ttgatagtac
    36001 agcaacatta tttgaatttc ctcgactacg agaccgcgga aacaaaacga tgaaatatat
    36061 cggtgaaatt actgtgaatg gttttccagg tatcgaaagt ggcgaaaaac cattttatga
    36121 agaaccacgt atgcctaaag atctaatcac aagaactgat tacgtaacgg ctaagaatgt
    36181 attagatgcg aagggtgccg atgctttggt cgaatggatc aaaggacagg aaaatttgtt
    36241 gttgacggat acgacattta gagacgccca tcaaagtctg ctagcaactc gtgttcggac
    36301 aaaggatttc aagcagattg cacgtttgac tggagaaggt cttcctgaac tcttttcaag
    36361 tgagatgtgg gggggagcaa catttgatgt ggcttaccgc tttttgaatg aagatccttg
    36421 gcaacggttg agaaaaattc gttctttaat gcctaatacg ttgctgcaaa tgcttttcag
    36481 aggttcaaat gccgttgggt attctaatta tcctgacaat gtattggtgg aatttgtcaa
    36541 agaagcagca gcgcaaggaa tcgatgtatt tcgaatcttt gatagtttga actggactcc
    36601 acaaatggaa aaaagtatcc aagctgtacg ggatactggt aaaatcgcag aggcagctat
    36661 ttgctatact ggcgatatca acgatccaag tcgggcaaaa tacaatgttc agtattataa
    36721 agacatggca aaggaactag aacaactggg tgcgcatatc attgcaatca aagatatggc
    36781 tgggctgctt aaaccgcagg cagcttatcg tttgattagt gaattgaaag aaacgacaga
    36841 tctgccaatc catctgcata ctcatgatac cagcgggaat ggaatcatca cttattctgc
    36901 tgcttcaaaa gcaggagtag acatcgttga cgtggcaatg agtgcaatga gtgggaatac
    36961 tagtcagcct agcatgagca gcttgtatta tgcactggtc aatggtccac ggctgccaga
    37021 aatcacgatt gaaaatgcac agaaattaaa ccattattgg gaagatgtgc gcatgtatta
    37081 caaaccgttt gaaaacggct tgaatgctcc tgaaacagaa gtatatatgc atgaaatgcc
    37141 aggaggacag tattcgaatc tacagcaaca ggcaaaagct gtcggattag gtcatcgctg
    37201 ggatgaaatc aaacaaatgt atcatactgt caacttgatg tttggcgata tcgttaaagt
    37261 aactccatca tctaaagtag ttggagatat ggcactattt atggtccaaa atgatcttac
    37321 agaagaagat atttatgaaa aaggcgaaac actaagtttc cctgaatctg ttgtgacttt
    37381 cttccaagga gaactagggc aacccgttgg cggatttcct aaaaaattgc aaaagattat
    37441 tcttaaaggc agacctgcat tgaatgaacg tccaggtctt ttggctgaat cagtcgactt
    37501 caacgaagta aaaaaagaat tggcagaaaa aattggctat gaaccaaaac aagacgaagt
    37561 attaagctat ttgatgtatc cgcaagtctt tttggactat caaaaagcat ataatcaatt
    37621 tggcgatgtt acattactgg acacaccaac atttttccaa ggtatacgtt tgggtgaaac
    37681 aatcaatgta caaatcgaac gcggaaaaac gcttatcatt cgtttagatg aaatcggaga
    37741 acccgatatc gaagggaatc gcgtactgtt cttcaatttg aatggacaaa gacgtgaaat
    37801 cactatcaaa gataattcga tcatcagtac ggtccaaacc cgattgaaag ctgaaccgac
    37861 aaaccgagaa caaattggag caacgatgtc tggttctgtt ttggatgtat tagtgaaaaa
    37921 aggcgataac gttaaaaaag gtgatacgtt gatgattact gaagcgatga aaatggaaac
    37981 ggccatcgaa gcacgttttg acggagaagt cgcacatgta tatgtgtctt caggagacac
    38041 tatttcgtct ggtgaccttt tgattgaagt gactgagaaa taataagcga aaggtgggga
    38101 aacatgaaaa gactgggttt gttttggctg attctgctat tagttattgt ggcattctac
    38161 atggaaccag tcttgtttcc gccaaaactt gaagtgaagg aaaactctga tcaacaaata
    38221 tcaaaccaaa tgacaacgtg gaaataccag gagcttaaag caactggttt ttctacttat
    38281 atcggaaaac cgatcgatca gctggaaaag gattttggcc aagcctatga tcgattaact
    38341 agcggatttg gttttgaaac aagatattat gaaaataaag ctagtcatct cacatttgaa
    38401 gcgaacatcc aagacagcag agtcacaact gttaaagtac tgcaaacagg gcctgctgat
    38461 atcgcgccgt tttatttagg gatgacgatg caagatctta cgaaaatcac tactatctat
    38521 actaatttca cttttgagta ccatgataca gaagttggaa tcgaactaat ggaagaggat
    38581 atgaattatc gtccgctaat tgcttttgat aacgacactt ttgctatttt attttttgat
    38641 caaacaacaa acggcctatt ttcagttgtg tatttagata aagatagtct tttgaaacta
    38701 ttgccatatc aagtgtttgg tgaaggcctt ccacattatc aagcagataa atcggctgat
    38761 tgggaatcaa tcaatcgcgt taaagaacga aaaagtacga ctttattaaa tatgcttaga
    38821 agagaagacg aacttcccat atacaatcaa acacttgcct ttcaaaacca cagtcagctg
    38881 ctactacatg attttttgac aaggcctgag gaaatattgt cagaggaacg tatggctgat
    38941 tggcaaaaat cgctaggcag tgctcaaacg acagtgaaat tctcactgac gaatgatgaa
    39001 ttggagaagt taatcagtaa acaaaaatta gaaaatacta gtgggctatt tattcatccg
    39061 gcgattgacg ctactttttc gtttttatat tggtacagcg atccttacaa ccatgaccgt
    39121 ttcatggacc cgggaacgga ttctgtaggc attgcttttt ctaaagaaaa tatgttagtc
    39181 ttattgcaag aagaaaacgg agaaagtagt gatagcagtg atcgttaacg aagagctgtt
    39241 tcaactggag gatcaatgcc tccaagtcgc tgaaatgata aaaaagagta aaacgatggc
    39301 ttcttatctt gaacataaac agtcaatgga agaatctgca aaagtttgcc agttgaaaga
    39361 agcgttcttg tctaagaaag aagcatttga aaatgtagcg gcatatggtg agtatgcacc
    39421 tgattatcgt gaaaaaaaac gagctgttcg aaaagcaaaa agacagcttg atatggatga
    39481 gcatgttgca gctttccgat atagtgaaac acagctgcaa agtctgttgg atcaaattgt
    39541 ccaagtactt gccgaagaag tttctaatga aatcaaagta gatgcaggta gcccgttttt
    39601 tgaaacgaaa aaacactttg gatgcggagg taattgtcat gcaattagac aatagtgaat
    39661 ttgaaataac aaaacgacgc gggctgatcg tatgggtcta cagcttaaaa caattaaaga
    39721 atctgaaaaa gttcggcttg ttgcattatg tctcaagaag aatgaaatat gtcgtgcttt
    39781 acttaaatga agagacattg gaagaaacag aagaacgaat ccgtaaatta cattttgttc
    39841 gaaacgtaga acgttcttac cgaccggata tcgaaatgaa ttttgccgaa aaaataggaa
    39901 acaaaacagt attaaaagac gaaggtttcg aaattgaaga gctaaataca gaaatccgat
    39961 tagctgaaca tctttaaaaa tctcttatga gcagttttta aataaaaaat aacttggata
    40021 acgatcgatt tttgatcatt gtccaagtat ttttttggta tactagtacc agtaccattt
    40081 ctaaggagtg agtagatgcg gataatatca ggaaattatg gcgggcgccg tttaaaaagt
    40141 ttagcagggg cgaacaccag accaactaca gataaagtaa aagaatccat attcaatatg
    40201 attggaccat attttgatgg agagacagta ttggatctgt ttgcaggaag cggtggttta
    40261 gctatcgaag cagtatctag aggctgcagt catgcggtat gcgtagacaa aaattaccaa
    40321 gcgctaaaaa tcatcaaaga aaacatcgaa attacgaaag aaccagagaa atttaccaca
    40381 ataaaacttg atgcggacaa agcaatcgaa cggctagcag cggagaaaca acaattcgac
    40441 tacttgtttc ttgatccgcc atatgcaaaa caaaaaataa tcgatcaaat ccataaaatg
    40501 gaagaactgg gacttttttc taaaaccgca gttatcgtat gcgaaacgga taaaacagtc
    40561 gaattgccag aaactgtggc tggattccat caaataagaa aacagattta tggcataacg
    40621 gccgtaacta tctatagaaa agagggaaat caatgaaacg tgcattattc ccaggaagtt
    40681 ttgatccttt tacaaaaggt cacttagata cggtggaacg tgcggctaaa ttattcgatg
    40741 aggtagtaat tggtgttttt atcaatactt caaagaaaag tttgtttccc ccagaagaaa
    40801 gaatgacatt gatcacaaaa gctgtttctc atcttcctaa tgtaaaagtc atgcatcaag
    40861 aaaatcaatt gacggtagaa acagcaaaag agattggtgc agatgcgttg atcagaggca
    40921 tacgaagtat caaagacttt gaatatgaac gagagattgc tcagatgaat catcatctat
    40981 atgatgaatt ggaaactgtc ttcttattag caaaaccaga atatagtcac gtgagttcca
    41041 gtattttaaa agaagtactg cactttggcg gagatgtgag cagctactta ccgccagtga
    41101 tcaacgaagc tttagctgga aagcgtgaat caaatgaaag ctaaacaatg gttgaaaaca
    41161 gtactgatta tcatatttgg aatgttgatc attggatttg tagtagttcc cttgccgtat
    41221 tatgtagaat caccgggagc cacgatcgat ctgaaagaac taattacggt caatgataag
    41281 gaagataaag gttctgggtc tttctctctt acctctgttg gtatcaggca agctacaggt
    41341 ttcttagctt taaaagctaa aatgacccct tttgaagaat tgatcagtga agaagaattg
    41401 actggtgggg cgacaaatga agaatataat caaatgcaac agtattatat ggattcttcc
    41461 caaaatgcag caatcgaaca agcgttaaaa cttgcagaca agccttataa aatggaattc
    41521 aaaggcgtat atgtcatgag tgtagagaaa acatcgaatt tctttggcaa gctttcagtt
    41581 ggagatacag taacaaaagt agacgggaaa agctttcaaa gtacagaagc atttatggat
    41641 tatgtaaaaa gccaaaaagt tggacaaaca atcgaaatca gttatttgca taatggtgaa
    41701 gaaaaaacag cttctggtga tctgatcgag ttaccaacag ataaaaaggc aggaataggt
    41761 atcacgctta ctgaccatac cgagatcgaa tcagatacag atgttcgtat taattctggc
    41821 tctatcggtg ggccatcagc gggattgatg ttcactttag aaatctatga acaagtcact
    41881 ggtaaaaact tgcgacatgg taagcaaatt gcaggaactg gaacgatcaa cagtgaagga
    41941 gaagttggcc gaatcggagg aatcgataaa aaagtggcaa gtgcggataa agctggcgta
    42001 gagattttct ttgcaccaga tgatacgatt tcagacgatg ttaagaaaga atatcctgaa
    42061 ataaaaacca actacgaaga agcaaaagct gccgctaaaa aattgaattc ctcaatgaaa
    42121 atcgtaccag taaaaacagt acaagaagcg cttgactatc tagagaaaat gaaatgaaac
    42181 taaataatta aagaggggtt gtgacacgag ttctgtcaca acccctcttt ttcgtcaaaa
    42241 atcttcacga taaaaataaa aacaaaagaa aaaattgacg taattagatg ttgaagggac
    42301 gattttgcca gagctcactg tttcttcaat tttaaatgaa aggagtaatg aatgattttt
    42361 actggtcatt ctttgaaaaa agaacaatga tagaaaaaat cagcaagaaa atactagctt
    42421 cacccaaatt gatcatttgg atgtctatga tagttcttat cttttgtatc gcaagtggtt
    42481 ttttattatc aaatttgcta ttttatagag aaacagaaga gactgctgca aaagaaatac
    42541 tttcttcgtt tagttccagt ggagaagatc catcagctga aggaaacgat gtagaagagg
    42601 atgctcgtcc aaaggtcatg tacaccgata tcaaaggaag tgtgaaggag cctggtatct
    42661 attccttttc ttcagaagaa cgggtctatg atgtactaaa gcgtgcaggt ggattattag
    42721 aagaagcgga tagcgatcga atcaactttt ctgcgaagat agaagatcag caagtcctat
    42781 atatccctgc tgtaggtgaa gagctgccag aacatcttga tcagttggca tcctcggaag
    42841 gaaaacagcc tacagcagat tccgagcctc ccaaaataaa tatcaataca gccactccat
    42901 cagaacttca gcagattcct gggatcggat cagtcaaagc gcaagaaatc attcgattta
    42961 gagaagaaaa cggatccttc cagaaagtgg aagacctgca ggagatttct ggtatcggtg
    43021 aaaagacagt tgaaaaacta aaaaacttcg ttacaattaa atagaatgat tcaaaatcga
    43081 ctggcacgag caataaatgg acgctaatgg atactaagaa taatccaatt ctaagagctt
    43141 ggcaggacga ggaggtagta tttttggata ggaaatagct tgattgttcg caaagaaaca
    43201 ttaaaaaaac caatgctgaa tgtctatctc caaaataaaa tatctgggat tcatattatg
    43261 aatacggctg tcagcggcaa taacagtcaa gctctacgag aacgctttgc caaagatgta
    43321 cttagctata cagcagataa agtatttatt ctcattggga cgaatgatct agcagagcat
    43381 aaacaacttt ctaaagaaac gtaccaaaaa atctgcagtg gatgatagtt cagctgaaaa
    43441 aagactatag ttctgatcat gctttcttta tcacgccacc agcagtcgat gagacaaaac
    43501 aaaaatatcg gaataacaag ttgatcaaag aatatatctc tgttctcaca gaagtagcaa
    43561 aacgccatga ttgtgaagta tcgattttta tacggaattt cttgaacaaa tggattcgaa
    43621 gaatttttat aatgggctaa tggatgacgg acttcatttc ggcagtgaag ggtaggatct
    43681 tttgtcagac aggatgattc gttatattta aagaatattt tgtttaagca atgagtactt
    43741 tttaatcaac ttgaaaaaac ggaggaatat aaaacatgtt agaaaaatct tcaaaagcat
    43801 gcgatacgaa taaagaaaaa gttactgata agtcacataa tgaacgcatt ccttgggacc
    43861 agtactttat ggctcaggca gtactgttat ctttacgtag tacgtgtaca cgtttagaag
    43921 taggagcgac actcgttcga gaaaaaagga tcattgctgg cggctataat ggagcagtca
    43981 gtggagatgt ccactgcata gatgaagggt gttacgtcgt ggatggacac tgcttgcgaa
    44041 cgatccatgc agagatgaat gcactcctac agtgtgcaaa acttggtatc ccgacggaag
    44101 gttcagaaat ctatgtcaca cattttccat gtttggcctg tacaaaggct ttgttgcaag
    44161 ctggtgtgaa agggatcaat tatctccatg actatcgaaa tgatccttat gcccaagcgt
    44221 tgatcgaaca attaggtgta tctgtacacc aagttgcttt agactcacag catttttcaa
    44281 aacttcaaaa tgaattaaca ggaaaacatg tctagcgttt tagaaaggca tgttcgaaat
    44341 cggttgattt ttccggcaat tacgtcgatg attatctgtt tgttgtatta cactgacaat
    44401 tggggttatc gtttgcttct tgccggaatt tttttatggt tttcgctaaa agaaggacgt
    44461 tttatcgcgt acttgtgtat tttgtgtagc ataacggtat ccatttctta taggctacaa
    44521 gagtattcat ttttagagga aaaaacaaca tctgcgcaaa ttactgtatt tacagatacg
    44581 attcgtgcaa atggtaattt tttgacaatg gaaggaaggt tatcagagaa taaacaaaag
    44641 ctccagcttt cttatcaagt aaacacagaa gcagaattga aaacatggct taacttccga
    44701 ggaaagggaa tagaattgac tgctgaaggc gagttcatct ctccagcaaa aaagcggaat
    44761 ctttataatt ttgaccaaga agcttacttc aggaaccata gaatttctgg acggttccaa
    44821 attcaaaaga ttgaacagat caccatgcaa aatcactggc gaaactggct tcagagaaaa
    44881 agaggatctt ttatctcctt tgtccaatct gaatttccgc aaaaaacaag tgtatatatt
    44941 aattctttat tatggggcta taaagatgca ggattcagag aaccagaaga aatttttagg
    45001 gagagtggtt tgctgcatct gttcagtcta tcgggtctac acatccaatt ttatttaggg
    45061 tggctgtatt atttatttcg acgtatcgga tggccactac acgtcagtat tattccgttg
    45121 agtctactaa ctatctgtta tgtttctatt gcaggaagtt ctatttctgt tttgcgtgct
    45181 agtttgctgt ttttattgcg attgctagtc aaattgctga ataagcatta ttccaccatg
    45241 gacttatttg cagttgtctt ttggcttctt ttactatttg atcctcgatt tctttttcat
    45301 gcgggtggac agcttagttt tttcatgagt tttttgttta tcctgatttc ttttgatgga
    45361 gattggttga aaaaaacggc ctctcacgtc ctgtttacta tgatcacgat gccgttgatt
    45421 gtatggcatt tttatgagtg gcctatttta ggtagtcttt ttacattact gattgcaccg
    45481 gtattcaagt ttctttttct tcctggtgtg ttatttctca cactcttcaa tcgctggctg
    45541 ccggaaactt tacttttgct gatagaagag ggattaatct tatttgagcg aataatcgat
    45601 ttttcttcag ggtcgtctct cattatagga cgtttgcccc ttgtgctatc tgggatgtat
    45661 attttcggat tactgatttg tatggatagg ttgaaagaac aacctgtgaa acacttgttt
    45721 tatgttggtc ttttttcttt tatttttttg actttgccat ttttacgctt ttcttcaacg
    45781 atcgctttta tcgatgtcgg tcaaggagac agtatctttt tgcaaagtcc ttttcgaaaa
    45841 gaaacgattt tgatcgatac tggaggtcag ttacactttg aaagagaaaa gtgggcgcaa
    45901 ggtatgatac gtcctcctgc tgaaagaaat atcgttccat atctaaaagg tcaggggata
    45961 agtgtaatcg ataaactcat cttgacacac gacgatacgg atcatgtggg agaattaccc
    46021 acgttatctc agcattttac tatcaaaaaa atttatatcg gttggggcgc tggacgaaat
    46081 gagccattac aaaggcattt atctgaagct caaaaaaatg gtactgagat cattgaaatc
    46141 aaacatgggg acaggatcag cggttattat gatctttatg tattgactcc ttttgaaaag
    46201 ggagagggaa gaaatgaaga ttcgattggt ctatggatgg aatataacaa taggcgcttt
    46261 ttatttttag gagatttgaa tcaagcaatg gaaaaacaat tgcttttgat ttatcctaat
    46321 ctgaaagccg atgtagtcaa attaggtcat cacggtagcc gtacgtcaag taacacagat
    46381 tttctttccc atattgaagc aaaacatggg attatatcct gcggcgtaaa taatcgttat
    46441 ggtcacccgc acagcgaagt attggagaca ctagaagaaa atcagataaa aacatggcga
    46501 acggatcaac aaggaatgat cacctatcgc tggcatccag tttttcatcc tcatgggcta
    46561 gtggaaacaa tgatagacta gtaattctca tctgctcatg gtaggataat ttttgaaagt
    46621 ggtgagtaat ttgagtatac aagcagagtt acaaaaaata cgtaaagaaa aacttgcatc
    46681 ttgttacttg attttaggaa cagaaaagtt tctccaagat caagttcgga cagaaattgt
    46741 caaaaaaatc caattagagg gcgaagatga cttaaatttt ttaagttttg acatggaaaa
    46801 tgcaagttta gatgaggtag tagctgaagc agagacgttg ccgtttttcg gagatcaacg
    46861 gttagtattc gtagagaatc cttatttttt aacaggtgaa aaagtaaaca atggaatcga
    46921 acaaaatact gatttattga cggcttattt gaaagaacca ttagaatcga ctgtattagt
    46981 attctttgcg ccatatgaaa agttggatga acggaaaaaa gtaacgaaac aattgaaaaa
    47041 aacagcaatt atcatcaatg tccaacagtt gaatgaaaaa gaagtacgtc aatatctgat
    47101 gaatacctta gagaattcct caatcaaaat ggaacgaagt gcaattgatt tattcttgag
    47161 gttgaccgac ttagatttat ccaaaatgat gagagagtta cagaaattgg tcctttatgg
    47221 acaaaatcaa cagcaaatca cggtaagaga agtagaagaa ttagttccta aaacgttaga
    47281 acataatatt tttgacatga cccaatatat tttaaaaggg aagacggaac aagctttgcg
    47341 tttatacgaa gatttagtta tgcaagggga agaaacaatc aaaattaatg cgattttgct
    47401 ttctcaatta cgtttatttt tacagacaaa atttcttgta aagattggtt atcagcaggc
    47461 aaatattgct gaaacattga aaatccatcc atatcgtgta aaacttgcca tgcaggaagt
    47521 aaaaaaattc gatgaacatt tgctggtaca gttgtttgat caattagttg agatggatta
    47581 tcaaatcaaa acgggacaaa tcgaaaaaga attaagtttt caactattcg ttttgcgcac
    47641 agggcagttg ttaagaaatc gataagaaga aagacgaggg caaagcatgc gggaaggtcg
    47701 tttgtttttg tccttttttt gacaaataaa aaaatcggcc gaagccgatt cttttattta
    47761 gcgatttttt tgcttaaacg agatttgtcg cggttagctt tgtttttgtg gattagacct
    47821 tttgattcag ccatatcaat agctttaaca gcttctttat ataacgcatc tacgttgtca
    47881 gcacctgaag ctacagcatc ttcaaatttt ttgattgcag tacgcatagc gcttgtttga
    47941 gatgaatttt tcacattagc gttttcgctt gtacgaacac gtttgattgc agattcaatg
    48001 tttggcatta gtttcacctc cgataactta agttcttacg cgtgttaccg cataattcaa
    48061 cattcattat tatacctaaa ggcagcgtgc tttgcaatat gaacatgaca agtttttttc
    48121 cttaacagtc ttttattagg attttttttc agtaaaaacg gatagtacaa gctgaaaaaa
    48181 atcaaaaaga cgctattgtg ggaggcaata gcgtcaaacc aaactagatt tttatttatc
    48241 tatttaaaca aagtttgaat gggaatgttt ggtactaagt tattatataa caaagagaac
    48301 cgtttgcaaa gttttttttg aaaagggata cgattccctg ttgttctttg tttttctatt
    48361 aagaaactgc agttatttta taaccagtat cctttgatca gtaaaggcta ttctggtgta
    48421 ccgattattt taatgtattc atcgatattt tttatcaaca ttttttttat gaccaactgg
    48481 ttccgacggt tccatttgcc acttacttcc agcacatatt tatttagggg caaacgtaag
    48541 atatattttg ccaaatagcg atctttaagc aggccattga tcacacggtc ctcaagctgc
    48601 aagttgatcc cagtcagcat cattggctgt tcttgtaaaa cgtacgtatc aattaaaaga
    48661 ccttcaaaag tttccacaag cgtcttcctt tcttttcaaa gtaacctgaa aacaggcact
    48721 aattcttcca ttttgatatt cgttgaagtg gatgcgatca ttctttcttt tgtccactgt
    48781 aatctagtat aacaaaaaag agcagaagag ccaaaaagaa aaaagagcat cgaattctat
    48841 aggaagtctc attggcacgg cctaaattat tacaaaacat tgattaaaaa aagcccctgc
    48901 atcgcaggag catcccctat atcgttttaa taggtcaatc aaaaagcagt atcaaacaca
    48961 ttttgtatca aatcaggaaa taatagataa gctaagccca gaatcaaaag taccgctaga
    49021 agaaaccagc cgattgttcc tatgaaatac tttaattttt cgaacattgt atcacctcgt
    49081 tgtcagtata tcacggatca gtaaattggg ggtgttaaga atctcctaaa atagacaaaa
    49141 tttgttctat tttcctaaag caaatacttg taagatacag taagtgatac cagaagcaat
    49201 gaccggtccg gcggcgattc ctcttaagaa gacaacacct aaaatcgtgc cgaaaacaag
    49261 tgcaacagtg atttgtggat cagaagcaat caatcctaca cccttggaag ataagacagc
    49321 cactagccca ccgcaaaaaa cagctaccca gccaataggg gatttaaagg cttctaatag
    49381 atcacgtaga ccgatttctc ctgttgcgat agggattaga atagccgctg tgatgatcgt
    49441 caccccaata ttgattcctt tgccttgaag ccaagggaaa aaggaacggg aggctggtat
    49501 gagcttcata atcaaaacaa aagctgccgc tatcatcaga ctttgatttt tagctaaaat
    49561 tgctacaagt aaaataattc ctaaaaaaag ccaactttcc atgaaatata ttcctcactt
    49621 tcttcgaaac aatcataaca tgtttttaga aagttacaaa agtaggaatt catccttcaa
    49681 gacctattca taagctgttt ttttcataaa ataaacaaaa tcataaaaaa acactttgct
    49741 tatttgacag aagacagccg cgctttgtat agtggacaag acttacattt ttttgaagaa
    49801 tcagaaagat caggtaaagg tcttagtaaa cgcaagccat tcagaataac caatattgtg
    49861 cttccttcgt gtcctacaac tccaagaggt agatttaaaa attgcaggaa gttagaagca
    49921 atcaacaaga gaataacaag aatcgaaaaa ataatatttt gtttgatgat ccgccgcatt
    49981 tttaaagata gccgatgact gtagacaaat ttatttaagt cgcttttcat taagacgatg
    50041 tctgcgatat ccatagcggc atctgtccct tgccccatag cgacacctaa agaagcggct
    50101 gctaaagcag gcgcatcatt gactccatca ccaaccatcg catttacttg gtaaagttca
    50161 ttctcttttt tgactagttg tgttttttct tccggagtac agcttgcata gtaatgttca
    50221 atccctactt gttcagcaat ggcctttgct gtgccagaat ggtcgccggt tatcatactt
    50281 gtatggatgt tttgggattt aaagtaggaa attacttgtt gggtatttgc ttttggaata
    50341 tctagcagac ctaaaacagc aattaactgc tgatctttag aaagataaat cacggttttg
    50401 ccttgatttt ccaaacgttc gatcatttcc gcaatctctt tgctgattat catcgtctca
    50461 ggatcatatg cgtgcttgcc aactttccat tgcgcaccta aataggtagt ttgtaatcca
    50521 aaaccagtaa tttcttctat tggtaagtat tggatttctt caggtattgt gcaatcgaaa
    50581 tattgcaaaa tcgcatgagc aagtggatgc gtcgtttttt gttccatagc aacaagaagt
    50641 tgttgtgcca aatccttttc atcaaagaaa aagctgtcag ttacaactgg ttttccttta
    50701 gtgagcgttc ctgttttatc aaaggcaatc gcttttaatt cagaaagctt ttctaaatgg
    50761 ataccgcctt tgattaaaat cccatttttt gcagaatttg aaagagcagc tagagaagct
    50821 ggtgtagcag atgcgacaag cgcacaagga gaagccacaa ctaataatac cattcctcga
    50881 taaaagcttt catcccaact ccaacctagg aaaaagtggg ggagaaggat cattagagga
    50941 acagcaagca aaatcaattt tacataatta ttttcgattc gttcgataaa tgaagctgtt
    51001 tgagttggcg tattctgcgc ttcttctact aattggataa tcttagcaaa gacagtttcg
    51061 gaactcgttt ttgttactcg aatcgtgatg ggatttcctt gattcagcgt tcctgcatac
    51121 atctcatcgc ctaaatattt ttctacaggc acagattcac cgcttaatgc agactcatcg
    51181 atcgttgcct gtgtgctttc taaaaagcca tctatcggaa cagctgctcc tttagcgaca
    51241 aataccaagt tatccacctt tagttgagaa acatctactt cttgcatttg tccattttct
    51301 tttaataatt gggctttttg tggttgaaga ttcatcaaag cagtaatttc tttttgactt
    51361 ttattagttg tgtattcctc taatgcaccg cttaaacaaa agatgaatgt caacattgct
    51421 ccttcaaagt aattaccgat caaacaggca ccaatagcag ctaaagccat caataaatcc
    51481 acattcagtg tccgatcatt ccacaattct ttcaagcctt ccgatgtttg tttatatcca
    51541 ccgatgataa tggcactgat ataaaaaagt ggggtgatgg taagagagaa tcggtcgaag
    51601 ataaaaccga agatcataaa taaaaggcag ccgattgttg ctgcaaaagc ttttttctga
    51661 ttagtcatcg ttattagacc tccttgtcaa aacaaggata gcataataaa aatgtaattg
    51721 ataataataa cagctaaatg agaacgataa tggttatcat ttggtgatta agcttgatgt
    51781 tgtttttatt atttataatg attattatta aagaaaataa aaaaccagaa catttctcaa
    51841 ataaga
//
LOCUS       DARBFO010000010        51275 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000010 DARBFO010000000
VERSION     DARBFO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.010"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            330..1175
                     /locus_tag="SCK51_000733"
     CDS             330..1175
                     /locus_tag="SCK51_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1251380.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="SCK51_000734"
     CDS             1456..2676
                     /locus_tag="SCK51_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEG1251381.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="SCK51_000735"
     CDS             2673..5393
                     /locus_tag="SCK51_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1251382.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="SCK51_000736"
     CDS             5377..6321
                     /locus_tag="SCK51_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1251383.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="SCK51_000737"
     CDS             complement(6390..7400)
                     /locus_tag="SCK51_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG1251384.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="SCK51_000738"
     CDS             complement(7580..7924)
                     /locus_tag="SCK51_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEG1251385.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="SCK51_000739"
     CDS             complement(7917..8351)
                     /locus_tag="SCK51_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEG1251386.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="SCK51_000740"
     CDS             8489..9226
                     /locus_tag="SCK51_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1251387.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="SCK51_000741"
     CDS             9219..10430
                     /locus_tag="SCK51_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1251388.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10634..11416
                     /locus_tag="SCK51_000742"
     CDS             10634..11416
                     /locus_tag="SCK51_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEG1251389.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="SCK51_000743"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="SCK51_000743"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEG1251390.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="SCK51_000744"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="SCK51_000744"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEG1251391.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="tadA"
                     /locus_tag="SCK51_000745"
     CDS             14132..15061
                     /gene="tadA"
                     /locus_tag="SCK51_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEG1251392.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="SCK51_000746"
     CDS             15018..16067
                     /locus_tag="SCK51_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEG1251393.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="SCK51_000747"
     CDS             16064..16342
                     /locus_tag="SCK51_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEG1251394.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="SCK51_000748"
     CDS             16317..16787
                     /locus_tag="SCK51_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEG1251395.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="SCK51_000749"
     CDS             16804..17106
                     /locus_tag="SCK51_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251396.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="SCK51_000750"
     CDS             17123..17494
                     /locus_tag="SCK51_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEG1251397.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="SCK51_000751"
     CDS             17466..17825
                     /locus_tag="SCK51_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251398.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="SCK51_000752"
     CDS             17997..19004
                     /locus_tag="SCK51_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1251399.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="SCK51_000753"
     CDS             19029..20213
                     /locus_tag="SCK51_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEG1251400.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="SCK51_000754"
     CDS             complement(20650..22233)
                     /locus_tag="SCK51_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1251401.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="SCK51_000755"
     CDS             complement(22811..22954)
                     /locus_tag="SCK51_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEG1251402.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23176..23655)
                     /locus_tag="SCK51_000756"
     CDS             complement(23176..23655)
                     /locus_tag="SCK51_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1251403.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23728..24012)
                     /locus_tag="SCK51_000757"
     CDS             complement(23728..24012)
                     /locus_tag="SCK51_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251404.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24090..24284)
                     /gene="ssrS"
                     /locus_tag="SCK51_000758"
     ncRNA           complement(24090..24284)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="SCK51_000758"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24356..25642)
                     /locus_tag="SCK51_000759"
     CDS             complement(24356..25642)
                     /locus_tag="SCK51_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEG1251405.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25780..26451
                     /locus_tag="SCK51_000760"
     CDS             25780..26451
                     /locus_tag="SCK51_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEG1251406.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26417..26905
                     /locus_tag="SCK51_000761"
     CDS             26417..26905
                     /locus_tag="SCK51_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEG1251407.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26918..27865
                     /gene="prmA"
                     /locus_tag="SCK51_000762"
     CDS             26918..27865
                     /gene="prmA"
                     /locus_tag="SCK51_000762"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEG1251408.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27866..28630
                     /locus_tag="SCK51_000763"
     CDS             27866..28630
                     /locus_tag="SCK51_000763"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEG1251409.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28863..31076
                     /locus_tag="SCK51_000764"
     CDS             28863..31076
                     /locus_tag="SCK51_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEG1251410.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31087..31533
                     /locus_tag="SCK51_000765"
     CDS             31087..31533
                     /locus_tag="SCK51_000765"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEG1251411.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31704..32963
                     /locus_tag="SCK51_000766"
     CDS             31704..32963
                     /locus_tag="SCK51_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEG1251412.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33052..33999)
                     /locus_tag="SCK51_000767"
     CDS             complement(33052..33999)
                     /locus_tag="SCK51_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1251413.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34393..35685
                     /locus_tag="SCK51_000768"
     CDS             34393..35685
                     /locus_tag="SCK51_000768"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEG1251414.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35700..37469
                     /gene="aspS"
                     /locus_tag="SCK51_000769"
     CDS             35700..37469
                     /gene="aspS"
                     /locus_tag="SCK51_000769"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEG1251415.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37578..38453
                     /locus_tag="SCK51_000770"
     CDS             37578..38453
                     /locus_tag="SCK51_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1251416.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38719..40602
                     /locus_tag="SCK51_000771"
     CDS             38719..40602
                     /locus_tag="SCK51_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEG1251417.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40677..40961)
                     /locus_tag="SCK51_000772"
     CDS             complement(40677..40961)
                     /locus_tag="SCK51_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEG1251418.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40951..41685)
                     /locus_tag="SCK51_000773"
     CDS             complement(40951..41685)
                     /locus_tag="SCK51_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEG1251419.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41814..42437
                     /locus_tag="SCK51_000774"
     CDS             41814..42437
                     /locus_tag="SCK51_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEG1251420.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42629..43750
                     /locus_tag="SCK51_000775"
     CDS             42629..43750
                     /locus_tag="SCK51_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEG1251421.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43757..46885
                     /locus_tag="SCK51_000776"
     CDS             43757..46885
                     /locus_tag="SCK51_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEG1251422.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46904..50098
                     /locus_tag="SCK51_000777"
     CDS             46904..50098
                     /locus_tag="SCK51_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEG1251423.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50244..50951)
                     /locus_tag="SCK51_000778"
     CDS             complement(50244..50951)
                     /locus_tag="SCK51_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1251424.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16786 a         8949 c        10640 g        14900 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc ttgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatctcaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggctt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cgggacagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg aaactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acagtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatat cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccctggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttat
    23041 tttacttgaa ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg
    23101 cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg
    23161 caggtcgtct acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc
    23221 agttcggaca acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc
    23281 aataaataaa cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg
    23341 attatcttct gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga
    23401 ggttacatta ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt
    23461 tgccatttca gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga
    23521 acgtgtgtca atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac
    23581 cgccttttta aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt
    23641 gtattgttgt aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta
    23701 gaacacttta gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa
    23761 tcggatactg aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa
    23821 gcgttgattg aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagcacc
    23881 gtaaaattgt aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc
    23941 ctgtttatta aataaataat agctaacgaa taataatact aaagtggcga taatcattaa
    24001 aatcctaatc atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa
    24061 gagcaaattg ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg
    24121 tagtattgat cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa
    24181 atgataaggc atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt
    24241 tcggtcaaca cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca
    24301 tatgaattta aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt
    24361 tttttggagg atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt
    24421 catatttccc tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat
    24481 attgctgatt gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt
    24541 tgagtgattt cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt
    24601 tgccctctcg aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg
    24661 gcgacaagca aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca
    24721 atttttcagc agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac
    24781 ctatatcttc ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct
    24841 caactaaacg ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt
    24901 gaaaagccga aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc
    24961 tttggacaca ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag
    25021 gagtcgatag tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg
    25081 cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc
    25141 gttctttatc ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt
    25201 tgacttcaaa gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct
    25261 cagtagtcgc tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct
    25321 gttttgtttt atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt
    25381 ttgcttcttc cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc
    25441 gaaatgcata attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag
    25501 gaggtccata tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt
    25561 ttccttcccc gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc
    25621 gataagccaa tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta
    25681 tatcatattt agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt
    25741 atccaactaa tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata
    25801 gatgaatttt tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat
    25861 ttggagcatg acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac
    25921 ctaggtccag aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg
    25981 gcaatgtatg aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg
    26041 aacatcatta ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc
    26101 gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc
    26161 aacggtccgg gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct
    26221 atagcagata gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat
    26281 gctttgccta gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt
    26341 gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg
    26401 ggttggagga aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga
    26461 aaaacaagtg acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga
    26521 gatcgttgtt cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg
    26581 gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca
    26641 gaaatctgtt tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg
    26701 gttggcaaaa tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg
    26761 gcaaagcgat ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca
    26821 ctgggataat caacaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac
    26881 ttatttaccg tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt
    26941 agaaactgaa agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag
    27001 tggtgtcgcg attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga
    27061 gattttagat aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt
    27121 tccggaaacg actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt
    27181 acctgagttt ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag
    27241 caactgggca acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac
    27301 ggttgcgcca agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact
    27361 agatcctggt atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc
    27421 actagaaaca gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt
    27481 gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga
    27541 agtagccgtt cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca
    27601 tgtatcagct aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa
    27661 cattttagca gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac
    27721 aggcacgctg atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat
    27781 gttagaacaa gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat
    27841 tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc
    27901 aaaagactat tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat
    27961 ggaacccgag gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat
    28021 cacggagatt actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa
    28081 ggaactgcca agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg
    28141 gatcgttcag aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc
    28201 ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat
    28261 tgctcaagag gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga
    28321 aacgaaacaa gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga
    28381 ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc
    28441 agatagtcga atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga
    28501 ttattttgta gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga
    28561 aacagcacct ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc
    28621 tttcagctaa cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc
    28681 aacaatttgt ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa
    28741 gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat
    28801 tatcaaacag atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc
    28861 ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca
    28921 tgggtcccga acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg
    28981 acggacagtt tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta
    29041 tattagcaga tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg
    29101 ttgaagatac agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta
    29161 tgttagtgga cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc
    29221 tagcagaaaa ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg
    29281 tcaaattagc tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc
    29341 aaagaaggat cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga
    29401 tcagccggat caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt
    29461 attaccgtat cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg
    29521 gtactgttga agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg
    29581 gacggccaaa acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca
    29641 acgaaattta tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg
    29701 ctgtattagg agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata
    29761 ttgccatgcc aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag
    29821 gaaatccggt agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg
    29881 ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga
    29941 cgaaacaatt aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt
    30001 ctgaatttat ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac
    30061 caaaaggaga cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta
    30121 tccatacaga tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc
    30181 ttgattacaa attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg
    30241 gcccaagtcg cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac
    30301 gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca
    30361 aatgtattac agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag
    30421 aagcactcga tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg
    30481 gagaagttag ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa
    30541 aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg
    30601 acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa
    30661 ttcgtatcag tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta
    30721 aaggcagagg gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa
    30781 atgtagcaga acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt
    30841 atgatgcaga tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc
    30901 aaacagtcaa tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata
    30961 aaatggcgac gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg
    31021 tggataaaat caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg
    31081 acagatatga gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa
    31141 gaaattggaa aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg
    31201 caaaaagatg tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa
    31261 caaggaaaaa tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa
    31321 ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga
    31381 ccagaaacag ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc
    31441 acagtcgcta ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct
    31501 gtaacgatca ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac
    31561 tatgaaacac gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg
    31621 caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta
    31681 ttttttatag gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag
    31741 atttaaaaaa attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg
    31801 acgctcccgt cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat
    31861 cagcagcaaa agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat
    31921 tagtattgaa gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc
    31981 accatcggaa aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc
    32041 gtatcttaga taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat
    32101 ttgatttaag taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat
    32161 cagcgatttc taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg
    32221 gaacgtttac ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa
    32281 aatgggcgca aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg
    32341 tggcattata tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag
    32401 tggcagctgt tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg
    32461 cattgatggg acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact
    32521 tttcgacgtt acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa
    32581 tgctacctgc gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca
    32641 gttgggttat tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag
    32701 attcctttat gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat
    32761 atgagatgat tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat
    32821 ggatcactct tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct
    32881 ctgtacctga acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc
    32941 gagcgctact ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac
    33001 agtgctgatc ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc
    33061 acgaaatgct tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg
    33121 gacattgtca gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg
    33181 attcttcatg taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat
    33241 ggggcgaagt acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg
    33301 atttcttttt cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc
    33361 tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag
    33421 ataagggaat aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac
    33481 atcgacatat ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc
    33541 gactgctcgg atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt
    33601 tggtgtgctt tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg
    33661 ttttagtgca tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc
    33721 tttatttggt acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt
    33781 ttccttcagt acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt
    33841 atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc
    33901 ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc
    33961 tgcgtagact tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc
    34021 tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca
    34081 gatatctctt gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg
    34141 aaagagcgag taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct
    34201 aaagtttcat tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac
    34261 gagcgttatt cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat
    34321 ttcattcacc cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag
    34381 ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa
    34441 tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat
    34501 aatgaaatgc gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat
    34561 acaacggata tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg
    34621 acgcttcgac cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc
    34681 ggcccagagt atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa
    34741 cgccctcaaa aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca
    34801 gaaaaccctg caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg
    34861 gggatcacgc aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc
    34921 tatcgtcaag cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct
    34981 aaacgccgcc ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa
    35041 aaatttgtgg cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat
    35101 tttgataccg ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat
    35161 atggttcgcg gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca
    35221 aaaatggggg cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa
    35281 ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg
    35341 actatggagg cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc
    35401 gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt
    35461 ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca
    35521 gataaagcga atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg
    35581 attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat
    35641 gaacattttg atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa
    35701 tggcacaaag aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta
    35761 cattaaaagg atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac
    35821 gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg
    35881 aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac
    35941 gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg
    36001 aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa
    36061 tcagtgacga aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta
    36121 aaaacatcaa attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg
    36181 actttctcga tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact
    36241 acctagttcc ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat
    36301 tgtttaaaca attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt
    36361 tccgcgatga agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa
    36421 caacgttctt aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa
    36481 tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag
    36541 caatggctcg ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc
    36601 taagcgatac agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg
    36661 gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg
    36721 atcagttagg tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag
    36781 aagaagacgg attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca
    36841 ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa
    36901 tcgtcgctgc tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg
    36961 atgaatcgaa attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag
    37021 aagaaggtcg atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc
    37081 cgttattggc tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg
    37141 gttatgaatt aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac
    37201 tagaaactct aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag
    37261 ctttagatta cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa
    37321 tgttattagc cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag
    37381 cagcagatgt gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga
    37441 atattgatgt aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat
    37501 acgcttcttt tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata
    37561 taaagcgggg cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga
    37621 ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt
    37681 atcagccagg acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt
    37741 tgtcaaaatc actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata
    37801 tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta
    37861 tcacagtcac tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg
    37921 atccaatcat ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt
    37981 tgagaaacgg cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa
    38041 caggaaagtc gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg
    38101 gaattctatt tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa
    38161 aagtaactga tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac
    38221 atccagaaaa agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca
    38281 atgaagcaga aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac
    38341 gatacgaact gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa
    38401 gtattgcaga aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt
    38461 ttgacaaaaa ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc
    38521 taccatagta gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga
    38581 aaaaaagcat gtttattttt ttaaatttat gggatgatag aaaaaaatag tagagaaagg
    38641 acacgattct acttgctatt ttcacttaat acccgtatat tacgaatgaa aaaagtttat
    38701 gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact
    38761 gggtttgatg ggctccgttc attagctgtg gtgggtgtta tcttctatca ccttctgcca
    38821 actagtatga aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg
    38881 atcacggatc tgctgcggca ggaatgggaa caaaatggaa aaatcaatat ttggcaattt
    38941 tatgtcagaa gaatgaagag actctatccg gcacttgttt ttatgcttgt gacagcttcg
    39001 gcttatatca ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc
    39061 agcttactgt atgtcaacaa ctggtggcaa atcaataacg gtttatctta tttcgatcga
    39121 tttgctaatg agtcaccgtt tacgcatatc tggtctttag cagtagaagg tcaaaactat
    39181 ttggtttggc cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag aaaatggatc
    39241 ttttatactg tgttaggtac atccttcctc tccgccatct tgatgatgat cttgtataca
    39301 ccaggaggcg atccaacccg tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg
    39361 ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa
    39421 caagcgaaaa atattctgaa tatcgctgga ggaatctcgc ttttggcact gattctttgc
    39481 ttcttctttt tagatgatca ttacaatttc atctattacg gaggaatgct cctagtgagt
    39541 ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg
    39601 ctgacaaatc cagtatttac ttggattggc aaacgaagct atggcattta tctgtatcag
    39661 tttccagtta tgatttttta tgaagcaaag gtgactgata ttgccgatca tgttttgttg
    39721 catactttag tggaaatcac attgattcta atcatcagtg aactttctta tcgatatgtg
    39781 gagcgtccgc tagcacgttt tgattatggt cgtacattcc aagtgatgaa aggatggttc
    39841 agcaaaccga tcatcagtaa gagcaaacca tgggttgttc caggcacatt acttgtagca
    39901 attgcactcg ttggtttcgt tacggcaccg aaaaacgctg taacagccga tcaaaaacaa
    39961 ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa
    40021 aacaccacta gttcggttga ccaagcgatc cttgataagt acgagttgtc caaggcacaa
    40081 gggaataagg cacaaaaatt agaactaacg gcatttggtg attcagttat gttggatgct
    40141 gcaacggatc tacaagaact ttatccaaaa gtagtggtag atggagatgt cggacgacaa
    40201 ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt gaaagacact
    40261 gttctgattg gactaggaac gaatggctca ttctcggaag ctcagtttga tagtgtgatg
    40321 gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg
    40381 caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa tatgacgctg
    40441 attgattggt acagctacag taatgaccat agtgactggt tttatgatga tcaagtccat
    40501 ccaaatcctg aagggatgac acattacatt cacttagtca gcgaagcctt gctaggagga
    40561 agttcaagta cgaacagcag tgagacaaac gtgacagaat gatagctaca aaaaacttca
    40621 ggcaaaaaca tttgtttttg cctgaagttt ttttatattt accgtgtttt taacattcat
    40681 atagattcag gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg
    40741 gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga aaatacttct
    40801 gcatggatca tcttaacagg tcttcttttt tgaagacgag tatatttagc accagtacca
    40861 ttgttatgtt cctctaatcg tctagaaaga tcggttgtat atccaccata aaatgtctga
    40921 tctttacaga ataatacgta aaaataatgt tcatttgcca tagagcatct cacgaatctc
    40981 tgcagtgtat tctccatcca tattgtaagt gatcaatggt ggagcgactt tgaatccgtc
    41041 taatttgcct tctttgattc cttcaactaa caaaatattt gcctcttttc caggttttgg
    41101 gtaaacaaat tggatccttt taggtgcaat tcgatagtct tccatgacct gaatgatttc
    41161 taagaaccga tcaggacgat ggaccatagt aaagcgtcca tttgttttta gtagcttact
    41221 tgaaatatcg atcacttctt ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg
    41281 tttattaggg ttcgttttat ttgttggcag ccctttgaaa taaggtggat tacagacgac
    41341 taagtcacaa gaattatgtc gaacgacaga taagctgtct ttcagatcga tggtgtggac
    41401 agtcatttgt tcatttaaat ggttaagcgc gatactacgt cttgccatgt ctgctagacg
    41461 aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac
    41521 tgcgccattt cctgcacaaa ggtcaacaat cttccctttt ttaggtacac gggaaaagtg
    41581 agcaagcagc acagcatcta aagaaaaaga aaacactttg gaactttgga taatcttgat
    41641 atcttctgcg tataattggt cgattcgttc gtcttcgtga agcatggatt ccctccaaaa
    41701 tgaaaaaaat agtcgtctgt attatactat ttttattaaa aagaaccaag gtggcttgtc
    41761 aaacctctat tttttatgca tactatgacc ataagaaggg aaaggaagag actatgtttt
    41821 atcgatttat gcgcggggta gttcgagtac tattagcctt agttaatgga aatgcccgct
    41881 atgagaataa agaagtttta ccgcaagatg agaattacat actagttgct ccccatcgta
    41941 cttggtggga cccattatat ctggcggtag ctgcagcacc caaggaattc agctttatgg
    42001 caaaagaaga actgttcaaa aatccagttc tgcgatttat attaactcga tccaatgctt
    42061 ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa aacacctgta aagattttga
    42121 aatcgacaaa tttgagtttg atcatgtttc ccagtgggac aagacatgct acagaactta
    42181 aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc
    42241 aaggtccgtt gacacttggg gatctgttca agcgtcagag agtgacagtg cgtttcggcg
    42301 aaccaatcga cttatcagat atcaagaaaa cggataaaga aggtatggcg gagatcgaac
    42361 gacgaacaca agcagctttt gacaaattgg acaaagaagt gaaccctgat ttcaaatatg
    42421 aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg gtcttttgaa tctactgtcc
    42481 tgttcagttc agactttttc aatgaaaacg agggagagac gtcaagtctg ccctcttttt
    42541 gcgttccaat atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt gttataataa
    42601 ttagaaggac attttttgag gtgattgaat gcgtttttta catacagccg attggcatat
    42661 cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag cgtcatgtac tatcttctat
    42721 actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag acttatatga
    42781 taggagtgtc ccagctgttg atgccgtaga attatacaat gaattgatgg tcgattggaa
    42841 tttaaaggaa aagatcccga tttttgctat atcaggaaat cacgatagca gtacgagact
    42901 ttcagcgggt acgccatggt tcagttattc gaactatttt ttgcgaacac agctagcaga
    42961 agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt actttgaacc
    43021 aattgcagcg agattatatt ttgaagatga caatatccga acgatcaagc aagcggtagc
    43081 caaggtgata gataaaatgc aggagctatt tgattcagaa aaaaaacaag tattgattag
    43141 ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg
    43201 gttggattcg atccctggtg aactacttac accgtttgat tatgtagcac taggacattt
    43261 gcataatcaa gctgctttga gacaggataa tgctcgctat agcggttccc cattgaaatt
    43321 ctctttatct gagatcaacc aaaaaaaagg cgtatggatc gttgacctga caacaggcct
    43381 ggagttgaat tttagggaga tcgaacccct gcatgatatt gaagaaataa cagcgagttt
    43441 caaagaattg ctggatcctg ccttttatca gtccgtcaat cgggagaatt acatctatat
    43501 ctatctcact gatagaacga tcatcccgaa tatgatgaac cagcttcggc aaatatatcc
    43561 tcgtattttg gggattgaac gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa
    43621 agtactagaa atgaaagatc ctcaaaaatt agcagaagca ttttttagtg aaatgacgga
    43681 agagcagcta actgcaaacc aagaaaaatg gctcggagaa acgttgcaag aaatcatgga
    43741 aatggaatga tcaagaatga aaccgaaaaa attacaactt aaaaattttg gtccttttat
    43801 caatgagacc gtagattttt cacgtttgac agaagcaccg ctttttttga tcagcgggaa
    43861 gacaggagct ggcaaaacga cgatttttga tgggatcacc ttcgcacttt ttggtgaaac
    43921 ttccggaaga cttcgctctg gaaaagagat gcgttctttg tttgctacac cagacgaaga
    43981 aacaagcgtg actttttctt ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc
    44041 agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac
    44101 gttgaccatt tttgatggga aaggaaaaga aataaaacaa atcacaaaac gtacagaagc
    44161 agatcagttg atcagagaac tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt
    44221 actaccccaa ggagaattcc gtaatttttt ggtttcatca agcagtgata aagagactgt
    44281 tttacgtcat ttattcggta ctcaattttt ccaacagttc aatgagcgat taaaagaaaa
    44341 agcaaaacaa cagcaacaaa aattggatca tttagagcaa gagctgcatc ttctgcaaaa
    44401 aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg tttgaagccg ttttagccca
    44461 atggacaaac catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag aagctctaac
    44521 ggaacttcag gaaaaacaaa agcaactaga aaattcctac tatcgttttg aaagcttgaa
    44581 aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa aaggaattag ctgctcaaaa
    44641 agacgaaata gaggaaaaga aacgatggat tgggtattat gaatttacgc aacgattagt
    44701 cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa atagaactgt cacaaaaaag
    44761 tagtgagcaa acaaagaaac tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa
    44821 tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa
    44881 caaacagatg atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca
    44941 attaacaatt tcattggata aagaagaatt gaatgccgag aagctgtatc aaaatctcgc
    45001 tatctatcag gaaagaactc gaattcttca agaaaattta ctggatcagg aaaaactgca
    45061 agaagatcgt cttgcttttg gacagttaaa atatctagat gaaaagcagc aagaaataga
    45121 aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata agagaagaac tcaaaaaagc
    45181 aactgaagaa ataacaaaaa atagtcagct attgaaagaa cagacagaac aagcagaaca
    45241 gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt cagctttttt tgcgtccagg
    45301 ggagccatgt cttgtttgtg gatcaacgga acaccccata caacaattaa aacacgaaca
    45361 gccaaataag gaagatatct taaaagtaga acaacaactg gcagaagctg agtctgctgt
    45421 agaaagaact cagcaaatca ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca
    45481 attatcagat ttagaacaaa aaatcatggc aacgaatata caattggctg aacaagaaga
    45541 acacttgcaa caggctttga aaaaggcatt tcctgagata acagaacaaa aaacaaaaac
    45601 aactcttgta gtaatcgaac aatctttaga tagacgaaaa agcaagatgg ccaatgaaag
    45661 aatagagctg gaagaaataa gacaacaagc agaaaagctc gaacaacaat tagctgtcta
    45721 caaacagcag cagacagaaa aaaataatca gctccagcaa ctgcaaggag aaatcaaaag
    45781 tttagaacaa caattgcaag atacggatag taactgttta gaagaggaga tacgacgact
    45841 tgaaaaacag ttagaagaag actcgaatca actagctgtt gagcagaaag caggagtgca
    45901 attgttgcaa gatcttcata cgcttcggac acagcttgat cttcaacatg aacaaataga
    45961 aaaaatagat gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt tagaaagcca
    46021 gtcgtatttt tcaacaattg aagaaatctg tgcatttgca caacagcaag cacaatataa
    46081 agaaaaccaa cgtacagtta cttcttatca tgaagagcaa ttagtgacag ctgaccggct
    46141 gcgtcaatta gaaaatatcc agctaactga gacattccca gatacagaag agatcagcca
    46201 acagttagaa gaaataagac gtatgatcga aataaagcaa gaaaagatat acggtttaca
    46261 agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt ctttcgattt atcacacaag
    46321 ccagacgaaa ttagatgagc ttacgcaatt acagcagctt tcgcaaacat tcaacggaga
    46381 aaatcctaag aagactagtt tggaacggta tgtgcttcag gtttatcttc aagaagtgtt
    46441 acaagtagca aatgatcatc tccaacgttt aacgaaaaat cgttatcaat ttgagctagc
    46501 ggataccatt ggcagttatc gagggaaaac cggattagaa atcaatatct atgatgatca
    46561 ggcaggtatg tctcgaagtg cgcataccct gtctggcggt gagagtttta ttgcagcact
    46621 ttctttagca ttagctcttg ccgatgtgat ccagacacaa gctggtggtg tagcaatcga
    46681 tgcattgttt attgatgaag gatttgggtc attagatgaa gaagctttgg aaatggcaat
    46741 ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag
    46801 agaattgaaa gaacgagtat tacaacaaat aagaattgat acagaaggta gcggacaaag
    46861 caaaatacgc tatcaacttg gataaaaagg gagattggta actatgaaat ggagtattcc
    46921 agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt
    46981 ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt
    47041 tgatttggat ggtacagcaa aagagataga ttactgccaa tgtccctatt gggaggaaca
    47101 taaatattgt aagcatacag ttgctgtgga actttattta cggtcgaaaa atctatctcg
    47161 tatcatgaaa aaagaccagc caatgctaga accggtaaaa gcaacaagtg agggagaaat
    47221 cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt
    47281 gattattgaa taccaagtag aaacgattga gactaatcag tatcacccag agctttctat
    47341 tttggcactt tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa
    47401 aaacatctat gattttttac aagcttacac agaaaaagaa acttacactg ttaataaaca
    47461 gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca
    47521 tcttgcagga agtgctcaga cgcatcaatt attaggtacg aacggcttac aagtcaaagg
    47581 aaaactagat aaaagatatc tgcttctccc agtagaacaa gcccgcttcc tgttagagca
    47641 gatgaatcag actacacgtt tgcatctgca gataaatgaa caaaacgatc aaggaataca
    47701 attttctgat caaaacaaac ctttatcatt caaagtggaa aaatttggag aagattattt
    47761 gctgcaagca ttgaatgatt ttgatttttt ctttatgcat tatcagtggg gagttatcca
    47821 agggacattt tattctctga cgaaatatca acaaacggtt tatcaaactt ggaaacaatt
    47881 gattcgccga ttggagaaac cagaagtagt gttcaagaaa aaagaattac cgtctttgtt
    47941 taaagagatt atccctctgc tgtcagagat cggcgacgta gctattgccc ctgcagtaga
    48001 agaggaagta gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa
    48061 aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg acccgaagca
    48121 tgaggtctcg acagaacagg cttttcaggt gttacgtgac acagtagaag agaaacgagt
    48181 acttgatcta ttcagaatgt atcgctacca gaaaaatgaa actggttatg aacgtccatt
    48241 gccaagcggg gaagaactat atgccttttt ccgcacagaa ctatctgtct tccgtcagtt
    48301 tggtgaagtc agacttggaa gaaaattgcg agagttgtat ttagatgcgc ataaacatca
    48361 accgaaaatc gaagtaaccg agaccggttc atggctcgat attcgattcg atgtgacagg
    48421 gatagaggag caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg atgcttttta
    48481 tacattagaa aatggtcagg ttctttcttt tgattcagaa gaatttcaac aaacaagcca
    48541 agtgttgcaa caattacggg aatcgatacg tacagaagaa ggaacgatcc atgtacctaa
    48601 aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc
    48661 gttccaaacg atggtacaag atctcattca tccagaaaga taccaggcac agcttcccaa
    48721 aggattgaat gcaacgatga gagattatca aaagcaaggt tttcgatggt tgaagatgtt
    48781 aggtcattat cagtttggcg gtattttagc cgatgaaatg ggacttggga aaacactaca
    48841 gacaattgcc tttttacttt cggaaaaaga agaacggaaa agcttctctg ctttgatcgt
    48901 agcgcctgcg agcttaattt acaactggca ggcagaggtg agaaaattcg ccccttcttt
    48961 aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg ctaaagacac
    49021 agatatccga gtgacttctt atgccagctt aagacaggat ctagctaatt atcagtcgca
    49081 aaaaattgat tatttgattc ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac
    49141 tgcacaagca ttgagagaat tagctgtacc tcagcggttt gcactgagtg ggacaccaat
    49201 cgagaataat ttagaagaat tgtggtcatt gtttgcaacg attatgccag gatttttccc
    49261 gactaaaaca aaattcaggg aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt
    49321 tattttacgg cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa
    49381 tctatattct gcacttacag aagaacaaaa aactgtttat ttagcttact tgcgtcaaat
    49441 gcgtgaagaa atcacatcaa tggattctga agcattcaag aaaaaccgga tcgggatact
    49501 tgctggtctg acaagactgc gacaaatttg ctgcgatcct cgtctgttta ttgaagatta
    49561 ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa
    49621 caaacgaaga gttttgctgt tttctcaatt tacagggatg ctgactatct tgcaagaaga
    49681 gttagccgag ctagggatct cgacatttta tttaagaggc agtacgaaac cacaagatcg
    49741 gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat gtttttctga tttccttaaa
    49801 ggctggggga actggattga atctcacggg agcagatacg gtcatcttgt atgacctttg
    49861 gtggaatcct gccattgaag aacaagctgc tggacgtgca catcggatcg gacaaaaaaa
    49921 tgtggtagaa gtgtggcgta tgatctctga aggaacgatt gaggaacgca tggattcact
    49981 ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag aacagttgac
    50041 gaaactgact gaagaagaca tccgcatgat tttaagtgta ggagaaatcg aggagtaaga
    50101 gactcgaaaa agcgttcagt aatgggtaag aaagcctttc taaagaagcg attgtttatt
    50161 tgatttaaaa aatagcaagg aggctgggat tccattttat cccagcttcc ttgctatcat
    50221 ttttttattt ctatttttaa aggttataca aagattgtct tcattgtctc taacagctga
    50281 tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg
    50341 ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta gaacatcatt gtcgtaatta
    50401 tcgccaacgt ataaggtctc ttctggaagc atatgaaatt ctttctcagc cagctgaaaa
    50461 atctcttttt ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga
    50521 atccaattgt tcaattgaag ttgcttcagt ttttttgtct gatggtcagt cggcccattt
    50581 gtgataattc ctaatgggac attcttttcc ttcaagaaat cgagggtttt tttgacctcc
    50641 tcatgcaagc agatattatc aagttcttcc tcgtaaattt tttgaaataa gaggccatct
    50701 tcttccgtaa tatgagggta gtctaaatct tttagtgatt ggctgatccg atgtgctcgc
    50761 atatattcta gtgtccaatc tcctgccatt acttttggga agttttcatc actatgatgg
    50821 cggaaccgga tatatagtgg atgcatatct gcatctgaaa caagcggaac gactcttttg
    50881 accgcgtttc gaaatggctg ctgctgatca tacatcgtat catcaacatc aaacacaact
    50941 gatttaatca aaattttaca cgcctttcgg ttttcattga ttttttcaca aatccttgac
    51001 tattgtaaca gaaaaaattg tctaaaaaag ctgataaata aaggtttttc ttactatatt
    51061 tctatttcct tgacagaata aggtttttat atttcagttt ttttcactaa aaatcgtgaa
    51121 acacgtcaat aagtttgatg aacaaattaa tcattatggt agaagtcgat tgatttattt
    51181 taatgcaatc tctgtggaat tttcgaaaga tttatttttt taatacagtc tcaatataac
    51241 tattcaaaca taaaataacg ttatttgatt tattt
//
LOCUS       DARBFO010000011        49184 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000011 DARBFO010000000
VERSION     DARBFO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.011"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            123..935
                     /locus_tag="SCK51_000779"
     CDS             123..935
                     /locus_tag="SCK51_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEG1251425.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            932..1732
                     /locus_tag="SCK51_000780"
     CDS             932..1732
                     /locus_tag="SCK51_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEG1251426.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1893..3161
                     /gene="rseP"
                     /locus_tag="SCK51_000781"
     CDS             1893..3161
                     /gene="rseP"
                     /locus_tag="SCK51_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEG1251427.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3229..4938
                     /locus_tag="SCK51_000782"
     CDS             3229..4938
                     /locus_tag="SCK51_000782"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEG1251428.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5150..9508
                     /locus_tag="SCK51_000783"
     CDS             5150..9508
                     /locus_tag="SCK51_000783"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEG1251429.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9653..10126
                     /gene="rimP"
                     /locus_tag="SCK51_000784"
     CDS             9653..10126
                     /gene="rimP"
                     /locus_tag="SCK51_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEG1251430.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10149..11324
                     /gene="nusA"
                     /locus_tag="SCK51_000785"
     CDS             10149..11324
                     /gene="nusA"
                     /locus_tag="SCK51_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEG1251431.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11346..11639
                     /locus_tag="SCK51_000786"
     CDS             11346..11639
                     /locus_tag="SCK51_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEG1251432.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11636..11947
                     /locus_tag="SCK51_000787"
     CDS             11636..11947
                     /locus_tag="SCK51_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEG1251433.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11960..14266
                     /gene="infB"
                     /locus_tag="SCK51_000788"
     CDS             11960..14266
                     /gene="infB"
                     /locus_tag="SCK51_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEG1251434.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14293..14640
                     /gene="rbfA"
                     /locus_tag="SCK51_000789"
     CDS             14293..14640
                     /gene="rbfA"
                     /locus_tag="SCK51_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEG1251435.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14797..14970)
                     /locus_tag="SCK51_000790"
     CDS             complement(14797..14970)
                     /locus_tag="SCK51_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1251436.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15418..17780
                     /locus_tag="SCK51_000791"
                     /pseudo
     CDS             15418..17780
                     /locus_tag="SCK51_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18148..19071
                     /gene="truB"
                     /locus_tag="SCK51_000792"
     CDS             18148..19071
                     /gene="truB"
                     /locus_tag="SCK51_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEG1251437.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            19075..20016
                     /gene="ribF"
                     /locus_tag="SCK51_000793"
     CDS             19075..20016
                     /gene="ribF"
                     /locus_tag="SCK51_000793"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEG1251438.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20368..21153
                     /locus_tag="SCK51_000794"
     CDS             20368..21153
                     /locus_tag="SCK51_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1251439.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21166..22017
                     /locus_tag="SCK51_000795"
     CDS             21166..22017
                     /locus_tag="SCK51_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEG1251440.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22141..22593)
                     /locus_tag="SCK51_000796"
     CDS             complement(22141..22593)
                     /locus_tag="SCK51_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1251441.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(23058..23426)
                     /locus_tag="SCK51_000797"
     CDS             complement(23058..23426)
                     /locus_tag="SCK51_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEG1251442.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23576..24058
                     /locus_tag="SCK51_000798"
     CDS             23576..24058
                     /locus_tag="SCK51_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEG1251443.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            24095..25105
                     /locus_tag="SCK51_000799"
     CDS             24095..25105
                     /locus_tag="SCK51_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEG1251444.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25220..25933)
                     /locus_tag="SCK51_000800"
     CDS             complement(25220..25933)
                     /locus_tag="SCK51_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251445.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(26123..26713)
                     /locus_tag="SCK51_000801"
     CDS             complement(26123..26713)
                     /locus_tag="SCK51_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEG1251446.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            27197..27424
                     /locus_tag="SCK51_000802"
     CDS             27197..27424
                     /locus_tag="SCK51_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEG1251447.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27440..28606
                     /locus_tag="SCK51_000803"
     CDS             27440..28606
                     /locus_tag="SCK51_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEG1251448.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28840..29883
                     /gene="hrcA"
                     /locus_tag="SCK51_000804"
     CDS             28840..29883
                     /gene="hrcA"
                     /locus_tag="SCK51_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEG1251449.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29977..30540
                     /gene="grpE"
                     /locus_tag="SCK51_000805"
     CDS             29977..30540
                     /gene="grpE"
                     /locus_tag="SCK51_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEG1251450.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30591..32420
                     /gene="dnaK"
                     /locus_tag="SCK51_000806"
     CDS             30591..32420
                     /gene="dnaK"
                     /locus_tag="SCK51_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEG1251451.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32571..33737
                     /gene="dnaJ"
                     /locus_tag="SCK51_000807"
     CDS             32571..33737
                     /gene="dnaJ"
                     /locus_tag="SCK51_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEG1251452.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(33819..34484)
                     /locus_tag="SCK51_000808"
     CDS             complement(33819..34484)
                     /locus_tag="SCK51_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1251453.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            34460..34633
                     /locus_tag="SCK51_000809"
     CDS             34460..34633
                     /locus_tag="SCK51_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251454.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(34678..35415)
                     /locus_tag="SCK51_000810"
     CDS             complement(34678..35415)
                     /locus_tag="SCK51_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1251455.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(35417..36919)
                     /locus_tag="SCK51_000811"
     CDS             complement(35417..36919)
                     /locus_tag="SCK51_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEG1251456.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            37178..39172
                     /gene="uvrB"
                     /locus_tag="SCK51_000812"
     CDS             37178..39172
                     /gene="uvrB"
                     /locus_tag="SCK51_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEG1251457.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            39182..41998
                     /gene="uvrA"
                     /locus_tag="SCK51_000813"
     CDS             39182..41998
                     /gene="uvrA"
                     /locus_tag="SCK51_000813"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEG1251458.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            42261..43526
                     /locus_tag="SCK51_000814"
     CDS             42261..43526
                     /locus_tag="SCK51_000814"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEG1251459.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            43530..44261
                     /locus_tag="SCK51_000815"
     CDS             43530..44261
                     /locus_tag="SCK51_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEG1251460.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            44396..45280
                     /gene="rapZ"
                     /locus_tag="SCK51_000816"
     CDS             44396..45280
                     /gene="rapZ"
                     /locus_tag="SCK51_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEG1251461.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            45277..46275
                     /locus_tag="SCK51_000817"
     CDS             45277..46275
                     /locus_tag="SCK51_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEG1251462.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            46309..47244
                     /gene="whiA"
                     /locus_tag="SCK51_000818"
     CDS             46309..47244
                     /gene="whiA"
                     /locus_tag="SCK51_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEG1251463.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            47787..48437
                     /locus_tag="SCK51_000819"
     CDS             47787..48437
                     /locus_tag="SCK51_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251464.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            48624..>49184
                     /locus_tag="SCK51_000820"
     CDS             48624..>49184
                     /locus_tag="SCK51_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HEG1251465.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        16466 a         8421 c        10575 g        13722 t
ORIGIN      
        1 ttaaaaaaag aaaaaattat tcagttttta aataaaaaaa caatgatttc ataggaaaaa
       61 cagttagata ttgctataat acttaagata gtgggaatct tttaagtata ggaggaaaaa
      121 gaatgttacg tttttttccg caaaaaaaca aatatattca agaaaaaagc gaatttcaat
      181 ttaataccga tggaccaatt cctcagcaca ttgccatcat catggacggc aatggtcgtt
      241 gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa agaaggcatg gaaacagtaa
      301 agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt tatgcctttt
      361 cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa ctacctgtcg
      421 acttctttga tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt catgtcatgg
      481 gttatgaaaa cgtattgcca gaacatacgc aagatgctgt aagacgtgca attgagcaaa
      541 caaaaaacaa tactggaatg gtattgaatt ttgctttgaa ttatggaagc agagcagaga
      601 tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa aggagaaata cacgcagaag
      661 aaatcgatga tgaattgatt gctaagcatt taatgacagg tttcttgcct aaagagttac
      721 aagatcctga gctgatgatc cgtacaagtg gagaagagcg aattagtaat tttctgttat
      781 ggcagatcgc atatagtgag ctgtatttta caaaagcgct ttggcctgat tttgatggtg
      841 cgcatcttga agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc ggcggtgtaa
      901 aaaaaacaga agatgagaaa ggggatcagt catgaaacaa cgtgtggtca cagcagccgt
      961 ggcgctagtc ttgtttattc cgattatatg gatcggaggc atagcggttg agcttacagc
     1021 agcaatctta gcagtaatag gagtttacga gctgtttcgg atgaaaggat tgacactctt
     1081 aagttttgaa ggagttttat ccgccgttgg tgcagtgtta cttgttttgc caaaagaaag
     1141 atggtttttc tttcttccag agaatgtaag tacatttatg ttattttatc ttatagttat
     1201 gattctttta ggaacatcag ttatttctaa aaatacgtat acgattgatg aagcaggttt
     1261 tcctgtgctt gtcagcttgt atgtgggaat aggtttccag aattttgtca atgcacgaac
     1321 agaaagcttg gctgttttga tatttggtct atttattgtt tgggcaactg atatcggagc
     1381 atatatggtt ggtcgacgtt atggtcagca taagttaagt cctgatattt cgcccaacaa
     1441 aacgattgaa ggtgcgctcg gtggtattgg tagtgctgtt gtagtcgcat ttctttattt
     1501 attgctatac ccagcaaaag aactatttaa tcatggtatg tttgtcatgc ttctgatgac
     1561 gattattttt tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa tcaaacgtca
     1621 ttatgatgtg aaagactcgg gaaatatctt accaggccat ggtggaatct tagaccgttt
     1681 cgacagtttg ctgtttgtat ttcctatcat gcatttattt ggcattattt agtaagtata
     1741 cctgcgtttc tttgtgaatt tcataaaaga cgcaggtttt tatttttcat tatattgata
     1801 ctattggtgt ctttggtata ctatggtaaa attatagtgt tttatcgttg ttttataaaa
     1861 aacacaaaaa acaagtaaag gagaaaaaag ctatgaaaac gattctgaca tttatcatcg
     1921 tttttggtat attagtgatt gttcatgagt ttggtcattt cttctttgcg aaacgatcag
     1981 gaatcctcgt tcgcgaattt gctatcggta tgggtccgaa aatttatggg catcaagcaa
     2041 aagacgggac aacatatacg cttcgcctat tacctattgg cggttatgtt cgaatggcag
     2101 gcaatgggga tgatgaaaca gaaatggcac caggaatgcc tctttctctt ttactaaatt
     2161 ctgacggcat cgtagagaag ataaatttaa gtaagaaaat ccaattgaca aatgctatcc
     2221 cgatggaatt gagccgttat gatttagaag atgagctgac tatcactggt tatgtgaatg
     2281 gagacgaaac agaagtagta acctaccctg ttgatcacga tgcgacgatt attgaaaacg
     2341 acggaacaga aatcagaatt gcgccaaaag acgtacagtt ccaatcggct aaattgtggc
     2401 agcgtatgct gaccaacttt gctggaccca tgaataactt tattttggca atcctgttat
     2461 tcatcattct ggcatttatg caaggtggtg ttcaagtgac gaacactaat cgcgtaggag
     2521 agatcatgcc taatggagct gcggcagaag caggcttaaa agaaaatgat gaggtagtca
     2581 gtgtggacgg aaaagaaatc cattcatgga atgatctgac aacagtcatt acgaaaaatc
     2641 cggataaaac attagacttt aagatcgaac gagaaggtca ggtgcaatct gtcgacgtga
     2701 caccgaaatc tgtagagtca aatggcgaaa aagttggcca acttggtata aaagccccaa
     2761 tgaatacagg ttttatggat aagatcattg gagggacgcg tcaagctttc agcggttcat
     2821 tggaaatttt caaagcctta ggatcactat ttacaggttt cagtttagat aaattaggcg
     2881 gacctgtcat gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta acaactgtta
     2941 ttagtttgat ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt ccgattcctg
     3001 ccttagatgg cgggaaatta gtcttgaata tttttgaagg gattcgcggg aaaccattaa
     3061 gtcaagaaaa agaaggaata ttgacattag ctggctttgg atttttaatg ttgttgatgg
     3121 tgctagttac atggaacgat attcaacgat tctttttcta gaaaaagttg acaaataggg
     3181 tataataaca aacgataact attttgagga aataaaggag tatatagaat gagacaatca
     3241 aaaatgttga ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt aagccatcaa
     3301 atcttgctaa gagctggata tatccgccaa gtatcagcgg gtatttattc atatctgcca
     3361 ttagcaaatc gtgtattgga aaaactgaaa cgcattatgc gggaagaatt tgaaaaaatc
     3421 ggagcggtag aaatgctgat gcctgccatc ttgccagcag aactttggga agaatccggg
     3481 cgttacgaaa catatggacc aaacttgtat cgtttcaaag accgcaatga tcgaaaaatg
     3541 atccttggac caactcacga agaaactttt actgatttga tgcgtaatga aatcaattca
     3601 tataagaaat tacctttaaa tctttatcaa atccaaccta aatatcgtga cgaaaaacga
     3661 cctcgcttcg gtttgcttcg cggaagagag tttatcatga aagatgcgta ttctttccat
     3721 gcaacggaag aaagtttgga cgagacatat aaagattacg aaaaagccta caccgaagta
     3781 ttcaagcgtt gtggcttaga atttcgctcg attatcggag acggtggcgc aatgggcggg
     3841 aaagactcaa aagaattcat ggcgatctca gaaatcgggg aggatacgat ctgttattcg
     3901 actgaaagtg attatgctgc aaacttggag atggctacaa gctactatgt atcaaaaaaa
     3961 tcgcatgaaa cacagcttga attggagaaa gtagctacac ctaacgtaaa atcaattgac
     4021 gaagtagcag atttcttttc tgttgaacct caaaaaatca tcaagtctgt attgttcatt
     4081 gcggatgaac aacctgtcct tgttttggta cgcggagacc atgaagtcaa tgacgtaaaa
     4141 ctgaaaaact ttttatctgc tgacttttta gaagaagcaa cagaagaaga agcacaaaaa
     4201 tatctaggtg ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga cgtaaaagta
     4261 tatgcagacc gttatgtcca agatttagcg aatgccatta ctggagcgaa cgaaacagga
     4321 ttccactata aaaatgtgaa tccagaacgc gatttcactg tactttctta tgaagatctt
     4381 cgttttgtac aagaaggaga tccttcacca gacaacaatg gtgttcttgc ctttactcga
     4441 ggaatcgaaa tcggacatat cttcaaaatc ggcactcgtt acagtgaatc tatgggagca
     4501 actgtactag acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta tggtatcggt
     4561 gtaagtcgtt tattatcagc aatcgtagaa caacattcag atgaacaagg aatcaactgg
     4621 ccaaaaggaa ttgcgccatt tgatttgcat atcgtccaaa tgaatctaaa agacgaatac
     4681 caaacaaacc tgacgaatga actagaagaa gcgatgacaa aagctggcta tgaagtactg
     4741 gttgatgaca gaaatgaacg cgcaggagtg aaattcgcgg attctgattt gattggctgt
     4801 ccaatcagaa tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt gaaaatcaag
     4861 aaaactggcg aaatggttga agtacgtaaa gaagaattgg tgaatacttt accaatcctc
     4921 ttgaaccaag gcgaataata agcgttaaaa cacagaaaat aaaaatagct gaatcagctg
     4981 tttttgacaa tctgatgatt cgcttttaaa attggaactg cgatttgaat cgcagttcca
     5041 attttttaat gtaaaactca gaaaaaacga gtatactaaa aggtactgaa aaagaagagg
     5101 ataagatgcg atcaattgca ttgtttctca tcagaaaggg cgtatatctt tgtcagaaaa
     5161 acgagattta tttgaaaaac tgttggaaca aatccaacta gaggaagcag aaaaaaatca
     5221 tccactgctt actgctggtg aaatggatag tgtagtggtc catcgtaaat cgcgtttatg
     5281 ggaatttacg ctgcattttt ccaaaatttt gcctattatg ctatatcggt cattgactca
     5341 gcatctgact atcgctttta aagatattgc acaagtcaaa ctgaatatct tagcagatga
     5401 ccagacattc gatgaacagc tcttgcaaga ttactgggcc caagctttgg aaaatcagca
     5461 atgcgataca ccactagcac agcaagtatt gaaaacgcag gtacctgtga taaaagatcg
     5521 taaagtcatc ttgccaatcg acagtacagg agctatttct tatttgaagc aacaatactt
     5581 gccattagta gaagagttgt tcgtcagtta cggattccca aaatttcgta tcgaaccaga
     5641 agtggatgag cagcaagcgg aacgagtatt gaaacttttt gaagaacgca aacaagaaca
     5701 ggcagaagcc tttatgaagc aagcagctga aagtctagtt gtccacgaac aaaagaaaaa
     5761 ggaaagaaaa gagcaatcgc cagctcttga aggacccatc cacatccagc taggacgaaa
     5821 tattccagcc gatgaaccaa cgacaccaat gatcagtatc gttgaagaag aacgaagagt
     5881 cacacttgag gggtacgtat tcgacaaaga agtcagagaa ctacgttcaa aacgcaagat
     5941 cttgactttg aaaatcacag attatacttc atcatttatc gtcaaaaaat tctcgaacgg
     6001 cgaaaaagat gaacaagtat ttgatgcgat cagtgtcggt agctggttaa aggtacgagg
     6061 aagtattcaa gaagatacgt ttgtccgtga tttggtcatg aatgcgcaag atatcataga
     6121 agtcaaacat acacctcgca aagattatgc tcctgaaggg gaaaaacgag tagagcttca
     6181 tgttcatagc aatatgagta cgatggatgc aacaaacagc atctctgatc ttgtggcaca
     6241 agcagggaaa tggggacacc gagctatcgc cattacagac cacggtggtg cgcaagcctt
     6301 tccggaagca catagtgctg gaaaaaaagc aggtgtgaaa atcttatacg gcgtggaagc
     6361 gaatgtcgta gatgatggtg tcccaattgc ttataatgat gcacatgaag cattgagtga
     6421 agccacatat gtcgtatttg acgtggaaac gacaggattg tctgcagtat atgacacgat
     6481 catcgagcta gccgcagtca aaatgtataa aggaaatgtg atcgaaagct ttgatgaatt
     6541 tatcgatcca ggacatcctt tgtcacgaac aacggtagat ctgactggta tcacggatgg
     6601 aatggttcga ggatcaaagt cggaagaaga agtattgcgg atgttccttg aattttcaaa
     6661 agatacaatt ttggtagccc acaacgcggc ttttgatatg gggttcttga atactagcta
     6721 cgcaaggtat ggtattcctg aagcggccaa tccagtcatt gatacgttgg aattagctag
     6781 atatctgtat cctcaattca aacgtttcgg attaggtgtg ttatctaaaa aatttggtgt
     6841 cagcttggaa cagcatcacc gagcaatcta tgatgctgaa gcaactggtc acttagcatg
     6901 gatctttgtg aaagaggcaa tggacaatca taacatgctt tatcatgatc agctaaatga
     6961 gcatatcgga gaaggagatt cttacaaaag ggctcgacct ttccatgtaa cgattttagc
     7021 aaaaaatcag gcaggactga aagatttgtt caaactgatt tcaatgtcga atgtcgaata
     7081 cttcgaacgc gtcccacgaa taccacgctc ccaattgaaa aagatgcgtg aaaatctatt
     7141 gatcggctca gcttgtgata agggagaaat attcgaagcc atgatgcaaa aaggggtaga
     7201 agaggcaaga aaccgagcaa aattctacga ttatattgaa gttatgccaa aagcggtcta
     7261 tgcgcctttg atcgagcaag aattggtaaa aaatgaacat gatttggaag aaattatcca
     7321 aaatttggta gaaatcggaa aaagtttgga taaaatcgtt gttgcaacag gtaatgtaca
     7381 ttatctgaac gaagaagatg ccatttaccg aaaaattttg atcaattcta tgggcggagc
     7441 gaatccgttg aatcgtcata gtttgccgga tgtccatttc cgaacaaccg atgagatgct
     7501 aactgcattt cactttttag gggaagaaac agcgaaagag atcgtagtag aaaatacgaa
     7561 taaaatcgca gatatctgcg aagaagtgat tccagtcaaa gatgaacttt atacaccgaa
     7621 gattccaggt tcagaagatg aaatatcaga attgagttac acaaaagcaa aacaaatgta
     7681 cggagatccg ttacctgaga tcattcaaaa acgattaaaa aaagagttga attctattaa
     7741 tggtaatggc ttctcggtta tttatctgat cgcccaaaaa ctagtgcata aaagtaatga
     7801 agatggctat ctagtaggtt cacgtggctc tgttggctct agctttgttg cgacgatgac
     7861 aggaatcact gaagttaatc cgttagcccc tcattattat tgtccagagt gccaatattc
     7921 cgagtttttt gaagatggta cgtatggttc tggatttgac atgccagaaa aacaatgtcc
     7981 aaaatgtggt gcacgcttaa acaaagacgg tcatgatatt ccatttgaga catttttagg
     8041 attccatgga gacaaagtgc ccgatattga tttgaacttc tcaggtgatt atcaagcaga
     8101 agcccataac tatacgaaag tattgtttgg cgaagactat gtttaccgtg ccggaacgat
     8161 tggtacagtc gccgacaaaa cagcatatgg ctatgtaaaa ggatacgaaa gggacaataa
     8221 tctgcagttt cgaagtgcag aagtcgacag attagcaaaa ggcgctactg gagtcaaacg
     8281 gacaaccggc cagcatccag gggggatcat cgttattccc gattatatgg atgtctatga
     8341 ttttacgccg atccaatatc cagcagacga tcaaaattcg gaatggaaaa cgactcattt
     8401 tgattttcac tcgattcacg ataatgtatt gaaattagat attctagggc atgatgaccc
     8461 aactgttatt cggatgcttc aagatttatc agggatcgat ccacaaacga tccctacgga
     8521 tgacccggaa gtgatgcgga tatttgccgg tcctgaagta cttggagtaa gtcaggaaca
     8581 aatttattcc aaaacaggga cattaggtat tccagaattc ggcactcgtt ttgttcgagg
     8641 gatgctagaa gaaacgcatc ctacaacttt tgcagaacta ctgcaaattt ccggactatc
     8701 tcatggtaca gacgtatggt taggaaatgc ggaagaattg attcgacgag gagatgcgac
     8761 actggcggaa gtaatcggct gtcgtgatga tattatggtt tatttgatcc atgctggatt
     8821 agatagcggg atggcattta aaatcatgga aactgttcgt aaaggacaat ggaacaagat
     8881 ccctgatgaa ttgcgagaaa cctatctatc ggcgatgaaa gaaaataatg tgcccgactg
     8941 gtatatcgat tcttgttcaa aaatcaaata tatgtttccg aaagcccatg ctgcagctta
     9001 cgtattgatg gctcttcgtg tcgcttattt caaggtttat ttccctattt tgtattactg
     9061 tgcatacttt tctgtacgtg cagacgactt tgatctagtc tccatgtgca aaggaaaaga
     9121 tgctgtgaaa caagcaatga aagaaatcac ggacaaagga ttagatgctt ccgtaaaaga
     9181 aaagaatcaa ctgacagtgc tggagttagc caatgaaatg ctggaacgag gattcaaatt
     9241 tggaatgatt gatttgtata aatcggatgc cgttaacttt gttattgaag gtgatacgtt
     9301 gatcgcacca tttagagctg tcccaagttt gggaaccaac gtggcaaaac aaatcgttga
     9361 agcaagaaaa gacggtccat ttttatccaa agaagatctt gccacccgcg gtaaagtttc
     9421 aaaaacattg atcgaatata tgaacgacaa tggcgtattg aaagatctac cagatgaaaa
     9481 tcaattatcg ttattcgata tgctataagc atctgttttc ctgtctgctt gtaattttaa
     9541 gcagagtatg gtatagttct tataggtaat gatactaact gtgagtgagc ggatttttcg
     9601 ctcactcttt ttaatggaat tacgcactta attttttaag gaggcgaaac ttttgagtag
     9661 cgtcgttgaa acagtcacag aaatggtgac accgatttta gatgaacaaa attttgaact
     9721 tgtcgaagtc gaatttgtaa aagaaggaaa aagctggttc ttacgagtat ttattgacaa
     9781 agaaggcggt atcgatatcg aagaatgcgc ctttgtcagt gagaaactga gtgaaaaact
     9841 tgatgcagca gatccagatc cgattcctca agcttatttc ctggaggtat cctccccggg
     9901 agcagagcgg ccattgaaaa aagaaagcga ctatgaaaag gcacttggtg attatgtcca
     9961 tgtctcctta taccaaccag tagatggcga aaaacaatat gaaggatttc ttcaatcatt
    10021 tgatgctgaa cagcttacat tgaaaatacg cataaaaaca cgagaaaaag aattgacttt
    10081 tgaccgaaaa aatattgcaa aagctcgctt agctatccaa ttttaatcac ggagaggaat
    10141 cagaaaaaat gagtaaagaa atgttaaatg cgttagacgc tttagaagct gaaaaaggga
    10201 tctcaaaaga aatcgtgatc gatgctttag aagcagcatt ggtttctgca tacaaacgtc
    10261 actatggcca agcacaaaac gtcgaagtgg aatttgatca gaaaaaagga aagatccatg
    10321 tctatgcggt aaaagaagta acggaagaag tgatggattc acagttggaa gtctcattga
    10381 aagatgcact tttaatcaat ccagcatatg agatcggtga tacgatccgt tttgaagtaa
    10441 caccaaaaga ttttgggcgg atcgctgcgc aaacggctaa acaagttatt ttacagcgtg
    10501 tacgtgaagc agaaagaacg attatttata acgaattcag tgcttatgaa aaagacatca
    10561 tgcaaggaat cgtcgaacgc caggataaac gttatattta tgtcaatctt ggtaaaattg
    10621 aggcagttct ttctaaacaa gatcaaatgc caaatgaatt ttatcaacca catgatcgaa
    10681 tcaaagttta tgtttcccga gtagaaaata cgtctaaagg tccgcaagta tttgttagcc
    10741 gcagccatcc agacctattg cgccgcttat ttgagcaaga agtaccagaa gtctatgatg
    10801 gaatcgttga aattgtcagc atcgcccgtg aagctggcga tcgttcaaaa gtggcagttc
    10861 gctcaaatga tccaaatatc gatgcagtcg gtacatgtgt cgggccaaag ggacaacgag
    10921 ttcaagcaat cgtcaatgaa ttaaaaggcg aaaacatgga tatcgtcgaa tggaatgaag
    10981 atccagctgt attcattgca aatgcattga atcctgcaca agtcatggat gtgatcttcg
    11041 atgagacaaa tccaaaggcc tgcacagttg tcgttccaga ctaccaatta tcgctagcaa
    11101 tcggtaaacg cggacaaaac gcacgtttag ctgctaaatt gactaaccat aaaatcgata
    11161 tcaaatcaga atctgacatg acggaatttt atgaaaatca agaacaacaa aaagaaacag
    11221 aagaactgca tgatgaagca atcgttcagt cagatttaac agacgatgaa tatgaaacaa
    11281 ttgcattcaa caatgaaacg gtcgaagaaa aaccagaagc ttaactttag taaggaggca
    11341 aagagatgaa acaaaggaaa attcctttac gcaaatccgt ggtttcaggt gaaatgaaac
    11401 ctaaaaaaga gatggtccga atcacccgtt caaaagaagg ggaagtatcg atcgatccaa
    11461 ctggaaaaat gcctggacga ggagcgtatg tctcacttga gccggaagaa gtacaacaag
    11521 cttgggataa aaaaattctc gatcgagtac tcgaaacaac attaacagat gagttttatc
    11581 aagaacttct tgactatgtc actcaccaaa aagcacgtaa agagttattt ggaaaatgaa
    11641 tacagttaat cggcaaaaag ccatgaatct catcggtcta gcgatgcgcg cgggaaaact
    11701 aatcacgggt gaagaactga ccattgctga aatccgcaaa caaaaagcaa aattggtgtt
    11761 tgtggcaacc gatgcaagcg agaatacaag aaaaaagata aaagataaga gttcatatta
    11821 tgaagttcct tgctttgagc tattttctga agcagaaatc acgcagatga tcggaaaacc
    11881 tcgcaaggta ttcggaataa tggatgccgg atttgcgaag aagaccaagg agctaattga
    11941 aggttaggaa ggtgattgca tgggcaacaa aagaatctat gaattagcaa aagaactcaa
    12001 aaaatctagt aaagacgtcg tggaaaaagc acaacaactt ggcatcgatg tgaaaaacca
    12061 tatgggtgcg attactagca atgatgagaa gaaattgcaa caagcgttca aaaacccaag
    12121 cacgaaacaa caagctgcac aacaagcaag cagaaaacca gccaatcaac agccgaacga
    12181 tcaacgtaaa aaagaaacaa cgaacaatca acaaaaaaat aaaaataacc gcagttatca
    12241 agatcgcggt caagggagcg gtcaagtgaa tcaaggaaaa aaaccaacaa caaaccaaaa
    12301 taatacggga aataccagca gccaaaatcg tcaaggaacg agccaaggcg gcaatcaaaa
    12361 ccgtactaac aataacaaca aaccaaacaa taacaatcgc ggcaaattta ataataataa
    12421 ccgtaatcgt tataataaaa aaggtaaaaa aggcaaacag caacaatcaa ctaaaccagc
    12481 tgtaccacca cgcaaattcc gtgaattgcc agatgtatta gagtatactg aaggtatgaa
    12541 cgtagcagat attgcgaaaa aaattcatcg tgagccagca gaaatcatta aaaaattatt
    12601 tatgcttggt gtcatggtca atcaaaacca agcactggat aaagatacga ttgaactgtt
    12661 agcgacggac tatggcatgg aaccacaaga aaaagtccaa gttgacattg ctgatatcga
    12721 taaattcttc gaggcagaag aagtcgatcc agataaatta gtctcacgtc cgccagtcgt
    12781 aacgattatg ggacacgtag accatgggaa aacaacttta ttggatacat tacgccattc
    12841 acgtgtaaca agcggcgaag ccggcggaat cacacagcat atcggtgcct accagatcga
    12901 tattgatggt aaaccaatca ctttcttgga tacaccagga catgcggcct ttacaagtat
    12961 gcgtgcacgt ggagcaagta tcacagatat cacgatctta gttgttgctg cagatgatgg
    13021 cgtcatgcca caaacagttg aagcaatcaa ccatgccaaa gctgcaggcg ttccaatcat
    13081 cgtcgctgta aataaaattg ataaaccagg tgcaaaccct caacatgtta tgcaagaatt
    13141 aagtgagtat gagttaattc ctgaagcatg gggtggagaa acgatctttg ttgagatctc
    13201 agcgaaattc ggccaaaaca tcgaagaatt gctggaaatg atcctgttag tcgcagaagt
    13261 agaagattta aaagcagatc caacacaacg cgcaatcgga acagtgatcg aagcacgtct
    13321 tgataaaggt aaaggtcctg tgtcaacctt acttgtccaa gaaggaacac ttcatgttgg
    13381 tgacccaatc gttgtcggta atacttacgg acgtgtccgt gtaatgacaa acgatcttgg
    13441 ccgtcgcgat aaagaagcag gacctgcaac gccagtagaa atcactggct tgaatgatgt
    13501 acctcaagca ggtgaccgtt tcgtagtctt cgaagacgaa aagacagcac gtgcagcggg
    13561 tgaagaacgc gcgaaacgtg cattattaga aaaccgtgct gccacaagcc gtgtgacttt
    13621 ggataactta tttgaaagcc tgaaagaagg cgaattgaaa gaagttaacg taatcatcaa
    13681 agcagatgta caaggttcag cagaagcact ggctgcatca ttgaagaaga ttgatgtgga
    13741 aggtgtccgt gtaaaaatcg tccattcagc tgtcggagca atcaatgaaa gtgatgtaac
    13801 cttagcagca gccagcaacg ctattatcat cggtttcaac gttcgtccta caccgcaggc
    13861 aaaagtgcaa gctgatactg aagaagtcga tattcgtctt caccgtatca tctataaagc
    13921 catcgaagaa atcgaaactg cgatgaaagg gatgctagac cctgaatttg aagaaaaaat
    13981 caccggtcaa atgatcgttc gtgaaacctt caaagtttct aaagttggta cgattgcagg
    14041 tgcctatgta acagaaggat atattcgtag agatagcggt gtccgtgtaa tccgtgacgg
    14101 aatcgtaatc tatgaaggcc aacttgcaag tctgaaacga ttcaaagatg acgtgaaaga
    14161 agtcaaaatg ggttatgaat gtggtgcaat gattgaaaaa ttcaatgaca tcaaagtaga
    14221 tgacgtaatt gaaggattta tcatggaaga agtcaaaaat gactaaaaac tgataaaagg
    14281 aggccaatca ttatggctaa ctaccgtgac cgccgagtcg gtcaagaaat cttaaaagaa
    14341 gtcaatgata ttttgcgcaa aaaggtccgt gaccctcgtg tggagaacgt aacgatcaca
    14401 gatgtgaaag ttacggggga cttacagcaa gcaacgatct attacagcat tttatctgac
    14461 ttagcatcag ataagaaaac agcacaagat ggattgaata aagcatcagg attgattcgt
    14521 cgagagttag ggcacaatct aagtatttat aagacacctg aattgttctt tgaacttgat
    14581 gaatcagttg tctatggcaa ccatatcgat gaattgcttc gcgacttgaa caaagactaa
    14641 tcaaaaactc ccgcaaatcc tgtgaaatca ggggttgcgg gatttttttg ttttcattaa
    14701 gacgtattac ctaagagttc catgacttta gtcttgcggc gtgtcagcta accaaaaaag
    14761 agaaaaagaa aagtaaaaaa ttcgactttc ttttttctag tagtatataa atttttgcat
    14821 atagataaag atcaagatac cgataacgga agctagattg taatctattt tagtgacagt
    14881 caggtatagc caagtaacta gaagatcagt ggaacctgca ccataaaagt ttgagtttgt
    14941 cggtccgttg acagttaaaa gaaaaatcac gttgaagtta ttgatatttc cgattacgcc
    15001 gcttcgattt ggtcacgatc caaattttgg ataatccctg tagaaattag catagtagct
    15061 gggataccaa ttcacatatt gacaaagata acagagactt ttacataaaa cggatcaatc
    15121 aggaaaggaa ttgctcgatc cagccccagt tttccagcaa tgtgttaact ggcccagcgt
    15181 cattcagcaa attatgcatg atgaggagcg aaatgaactg cggaacagcc attgtgataa
    15241 cgacaagttt tttggatcaa tgtaaaaaac gtcagcgaaa ggaaaaattt cgtggttttt
    15301 tatacctgtt catttggcta gatataaaat ccaaacaaat gaacagaagt gcttattagg
    15361 tttcaattgg gagtatctgc gctagaataa gagtaataat gaaaaggagt gttatgtatg
    15421 gattattcta gtaaagaata ctttgataaa atgacggctt ggtggcgtgc tgctaattat
    15481 ctttctgttg ggcaacttta tcttaaagat aaccccttac tgagacgtac actgaaaccc
    15541 gaagatgtca aaaaacatcc aatcggtcat tggggaacga ttcctggcca aaattttatt
    15601 tatgtccatt tgaaccgagt catcaataaa tatgatttga acatgtttta tattgaaggg
    15661 ccaggacacg gcggacaagt catggtatcg aatgcttatt tagatggaag ttacacagag
    15721 atctatccag aagtaacgga agatgagaca ggaatgcaaa aattattcaa acgattttca
    15781 tttcctggag gcattgcttc tcatgcagca ccagaaacac caggttctat ccatgaaggc
    15841 ggagagcttg gatactctct ttcacatgca gtcggagctg tattagacaa tcctgaagta
    15901 atctctgctg tcgtgattgg tgatggtgaa gcagaaaccg gtcctttagc tggaagctgg
    15961 ttctcaaatg tctttatcaa tccagtgata gatggagctg ttttaccgat ccttcattta
    16021 aatggtgcaa aaatagccaa cccaacgatc ttggcacgta aatcagatgg agaattagca
    16081 aattacttta acggacttgg ctgggaacct ttctttattg aaggaaatga ttcagaaaaa
    16141 ttaaatcctg ttatggctga aaaaatggat caagcaatcg agaaaattaa atccatccaa
    16201 aaagaagctc gcctgaagac agcaacagat gtcgtgatgc caaaatggcc agtattgatc
    16261 gtacgcacac caaaaggatg gaccggtcca aaagaatggg acggggaacc aattgaagga
    16321 accttccgag cacaccaagt accgattcct gtagatcaag aacatatgga tcatgcagat
    16381 gctttactcc gttggctgaa atcctatgaa ccagaaaaat tatttgatgc acaaggacgt
    16441 attttagaag aaatcagaga aatcgcgcct actggtgacc aaagaatggc aaaaaatcct
    16501 atcaccaatg gtggaatcga tccaagaccg cttattatgc cagattggaa aaaatatact
    16561 ttgcaatttg aaaaaccagg ctccgttaaa gcagaagata tgaccgaact tggtaaattc
    16621 gttcgcgaga tcattgagaa aaatccagaa aatttccgta tatttggacc agatgaaaca
    16681 aaatcaaacc gattgaatca agttttcaaa acgacaaatc gccaatggat ggaaaaaatc
    16741 gaaccagaaa atgatgaatg gctttcgcca tctggtcgcg tcatcgacag tcaattatcg
    16801 gaacaccaag atgaagggtt cttagaaggg tacgttctga ctggacggca tggttttttg
    16861 caagttacga atcattcttg cgagtggttg actctatgtt gactcagcac ttcaaatgga
    16921 tgcggaaatc acatgacctt tcttggcgaa atgattatcc ttcgctaaat ctcattgcaa
    16981 gctcaacagt cttccagcaa gatcacaatg gatatagcca ccaagatcca ggaatattga
    17041 cacatttggc agaaaagaag gcggagttca tccgtgaata tctgcctgcg gatgcaaata
    17101 cattgctcgc agtcatggat aaagcattcc gctcgtcaga aaaaatcaat ttgatcatct
    17161 cttccaaaca tccgcgtgct caattttact cagcagaaga agcagccgtc ttagtgaatg
    17221 aaggcttgaa aatcattgat tgggcttcga cagcaaaaga ggaggaacct gaattggtca
    17281 ttgctgctgc aggaacggaa tctaacttag aagcattagc agctgtaact ttgctgctgg
    17341 aagaattccc aaaactaaaa attcggttca tcaatgtcgt agatttattg aaattacgtc
    17401 atccaagtca agatccgcgt gggttgagcg atgaagaatt tgatcaatat tttacaaaag
    17461 acaaaccgat cttatttgct ttccacggtt atgaaacatt ggtccgcacg attttctttg
    17521 atcgtcataa ccatcattta atgattcatg gctacaaaga aaatggagat atcacgacac
    17581 catttgacat gcgtgtagta aatgaacttg atcgttatca tttggcaaaa gacgcagctt
    17641 taaaaatcaa aggaagtcaa gcagaagatt ttgcgaaaaa aatggatcag aaattacaag
    17701 aacatcaaaa ctatattcgt gaaaatggta ttgatctgcc agaagtattg gattggaaat
    17761 ggaaaaatct tgaccaataa aatgataaaa tcgatcccgc aaaatagaat tttttacatt
    17821 gattaagaaa ctgaaaaaat aaataaaaaa aacggaaaaa agactggaag aatggccagt
    17881 cttttttttc gtttttggga agtgaaaacc aaactattta cctgtaagcc acccataaat
    17941 ccaatcaaat ggtgcaaaga aaaaatcttc cattctattc cctcctttaa aaaagcataa
    18001 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
    18061 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
    18121 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat tacctttatg gaaagaacgc
    18181 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
    18241 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
    18301 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
    18361 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
    18421 gatatgtctt tatctgaaaa aaaaatagat gaagtgatgg cttcatttat tggtgagatc
    18481 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
    18541 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
    18601 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
    18661 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
    18721 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaggat
    18781 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
    18841 ctgccaatag aaaaaggagt ggaacggttc aaacgagtag atattactga tgaagtatgg
    18901 cagaaagtaa aaaacggtat gagacttgat tatactgtat ttgcgctccc agaaatgccg
    18961 tcagaagaaa ttgctttgtt ttataaagga aaagttgtca gtatttatca gccaaacccg
    19021 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
    19081 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
    19141 gtgctgggat ttttcgacgg cgtgcatatc ggacaccaga aagtcataaa gacaggtatg
    19201 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatct
    19261 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
    19321 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
    19381 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
    19441 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
    19501 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
    19561 ggtgaaaaaa tcagctctac ccgtattcgt ggattactag acagcggaca agtaaaagaa
    19621 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
    19681 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac ggttcatttg
    19741 cctaaagaag gtgtttatgt gtcggaaata aaaattgggg aaacatggta tccagccatg
    19801 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agagttgtat
    19861 attttggact ttaatcaaga tatctatggt gaacatgtcg cggtacgctg gaatcaattt
    19921 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaggaggat
    19981 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
    20041 ttatgtataa ctagcaatta caataaaatg ggtgataaat aagcccagga aaggggctgt
    20101 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
    20161 cggaaataag ccgaaatttc ttgaagctga ccacttctgt cacaacctct ttctttatag
    20221 aaataaaagg acactagcaa ttcatgaaaa cgattgctag agcgtatcat ttgctttaga
    20281 ctatatatct tggtgtacac tcactttaag gcggaaataa aagttatccg taataacagg
    20341 tttaggtatg aaaaaggagt gggtactatg tacaaggaat tagaaaacaa agtcgcagta
    20401 gtaactggtg gctcaaaggg gattggaaca gcgattgctc aacgattcgg gaaagaaaaa
    20461 atgaatgtag ttgtcaacta taactcggat aaagaaggag cagaaaaagc agtcaaagca
    20521 atcgaagagg caggcggaaa agcagtctct gtacaagcag atgtcagttc tgaagaagga
    20581 atacagcgtt tacttgatac ggctttagaa aattttggga cactggacgt ttgggttaat
    20641 aatgctggga tggaaaatca gcatccaacc catgaactta gccttgaaga ttgggaaaga
    20701 gtgttaaaag tcaatttgac tggcgttttt atcggaacga aagcggcttt aaattacttt
    20761 acagagaata ataaaaaagg aaatatcatc aatatgtctt ctgttcatga acagatccca
    20821 tggccaacgt ttgctcatta cgctgcttca aaaggcgggg taaaattatt taccgaaacg
    20881 gtagcaatgg agtatgcacc gaagggaatc agaatcaata gtataggccc tggtgctatc
    20941 cgtacaccta ttaatgcggc aaaatttgat gatcctgaac aaaaagaact cttggaaagt
    21001 atgatcccac taggacgcgt aggtgaacca aaagaaatag cggcgacagc tgcatggttg
    21061 gcttcagacg aatccagtta tgttacaggt attacactat ttgcagatgg tgggatgact
    21121 ttatatcctt ctttccaagg tggaaaagga taagggtgaa taaaaatggg tttattgatt
    21181 gcattgatcc cggcagttgg ctgggggatc cagccactga ttttgaaaaa gattggtggc
    21241 agaccaacaa atgagatatt aggaactggt attggagcgg tgcttgtcgg cttactcgtt
    21301 caattattcg cttctccagc tggtatttca gtgactacat ttttgattag tttactttct
    21361 ggtgcatttt gggttattgg acaaacagga cagtatacat ctttcaactt gattggtgtt
    21421 tctaaaacga tgccaatttc gactgcatta cagttagtcg gaacctcgtt gattggtgtc
    21481 ttcgcttttg gagaatggtc tggaagtatg gggaaaatca ttggagcagc agcaatcatt
    21541 cttcttgtat ttggttctgc tttaacggca atcagtgatg atcctggaaa aaaaggcggt
    21601 atcacaaaag gaattagtct attagcatca acaagtattg gttattgggt atacagtgca
    21661 ttgcctaaac tagttgatgc aagcggactt tccattttct ttccgcaaat gatcggtgtc
    21721 tttttagggg cagtgattta tgtgattttc cgtaaaccaa ccgcattttc tgaagacaag
    21781 agttggaaag caacgatcgt tggagccatc tttagtgtgt cagcattggc ttatattttt
    21841 tcagcaaatg aaaatggggt tgctactgca tatatcatta cacagctaaa cgtagttatc
    21901 tctacattag gcggtatgta catcctgcat gaaaataaga caccaaaaga aaaaaaatta
    21961 acgattatcg gattattgct gatcgtagta ggaagcatgg tcacggtctt tatttgagga
    22021 taaagatggt aaaatttcat ttattaaaaa aatatttgtt tgaaccaaac agtaagtact
    22081 tataaaaaaa tagcgctaga atttccaaca tctctggaat ttctagcgct ttttaattgt
    22141 ttaagataca agggaaatat ctgtctggat caataatttt tcattgatcc attgactaaa
    22201 gtttacgaca taattgaagt taccaccgat tagtatctcc gcatgattgt ctttataaag
    22261 atcgtattgt aaaagacggt cttttttatt tgcgtaaaca tcgttatggt actggactaa
    22321 ttgttctcgg aattgattga ttgctgtaaa atcacgatat tgaaggttga aacgcatcgt
    22381 gattggtgca tttagcatga atcgctgcat ttcttttgct gcttcctctg cttgatctcc
    22441 gtataggaac tcaaaaagat tacgttcaga aaatttccat tgcccgccaa cctttgttcc
    22501 ttttagtttt ccttcatgaa gataatttct gatagaacgt gtagtaacac ctaattgttc
    22561 ggctaattca tttactgagt aaaaatcttt caatgttcac cctcctatgt gaatggttct
    22621 actgacttag ttgatatttg cttaatcacg aaatgtagaa agaacgattt taattaaggt
    22681 cattgtaaaa atcaagtcag aaaattctaa cttttatttc tctaaagaaa ttcgttctct
    22741 cctaaagtga tattatattt aaatatacct gaaaataatg ggcagtatta tgaaagatca
    22801 ctgatatttt ctcgtatttt ttccttattc aaaagttttg caggaaaatt gctgaaaaaa
    22861 taattttact atgtagaaaa ttcaaagcca ttcaatcagt gatttttttc agcacaagaa
    22921 agactcctta taaaaatagc agatactttg tcaattaagt gtttaaccag ggatgataaa
    22981 aaggatagga ttgaacaaaa cctgtataag gtgatgaata gtttcctcgc cagtaaaagg
    23041 tttattcgta taaagcttta tcgccaaaat ttatcatgac cttttttatc ttcaatatta
    23101 aatgaaatca attgttcaat caattcccag ttgattacac ttttccaagg aactttgatg
    23161 acgttatccg tatatacgta gcctgcttct tggatatttt tttcaagggc acgaatcgct
    23221 gctttttccg gtgcgataga aaaatgattt ttagcaaaac taaatgcaat aataaaggta
    23281 ccatggtcag taaacatagg ttggttccat tttacagttg tgtctagctg gggatacgtt
    23341 tctgttatct tagcaaaaag gtctttgatc ttttgttgat gttctttttc tttgatttga
    23401 ttgatgatcg gctgaaattt ttccattcta aaacgcctcc attaagtggt ttgatttatt
    23461 ttaccagaaa aacaatagtt atgtataaaa aacgaacaaa acacaagtaa tgttgtaacc
    23521 gctatattta tatgataaga tgtctcaaat aataaaatag aaaagagaca taaaaatgat
    23581 tgacttggca aagaaattct tacacgaaaa tatatatttt gagttgtatc atcatcgagc
    23641 catttataca aatgaagatg ctttggtaat aaagaaagaa caaggattta caggtacaga
    23701 gaccaaaagc ttattcttaa aagataaaaa agataataac tacgtatatc tgacttttac
    23761 tacgaaacaa acggatttca aagcattagg gaagatcgtc ggtaaaaggc tttctattgt
    23821 ttcatcagaa cgcatggaag aacaaacggg acaaaagcag ggcgctgttt ctccgtttgg
    23881 ttatgaaaaa cagattccga ttattattga tgaagagctc ttatcaaatg aaaaacttgt
    23941 ttttgcacca ggtagaccag atcaaacgat ggtcatccaa acggttgact taaacaaaat
    24001 catccagcta cttgagatgc cgatattttt gatgccggca aacgaaaaaa acaattaacc
    24061 aagtaaaata gtgcatactt ggaggtgaag caacatgata agttatccat tattagaaag
    24121 aaatgcaacg attggggtta ctgcaccatc ctcaggcgta tcagaggaac accattcgtt
    24181 actcgaatca gctttaaaga gaatggaaga aaaatctttc caagtagtta ctggaaagac
    24241 gctttggacg caagataagg aaaaatcagg tccagcaaga aaacgagcag atgaattcaa
    24301 tcaaatgatg caagatgaaa atatccaaat gatctttccg acttggggag gagaattatt
    24361 gattgagatt ctcgattttg tcgattttga tgctctacaa ccaaaatgga tagtaggtta
    24421 ttcagatatt agtacgttgc tacttgctgt cacattaaaa acagggatag ccactgcaca
    24481 tggaactaac cttattgatt taagaggaga gtacttggat gaaacgacag caatgtggga
    24541 aagtgtactg aaaacaagag aaggagaatc gattctacag caatcttctg aaaagtatca
    24601 aaaagtgtgg ccccatgagc attcatcatc ttctgttttc catctcactg aaccaacaaa
    24661 ttggcaatca gtcagcggag atgcagtaag gatgaaggga cgattattag gtggttgtat
    24721 tgatacgatc aggcatttaa tcggtactcc ttatggagat gtccaatctt ttaaaagaaa
    24781 gtataatgat ggaaagccga tcttgtggtt tctagaaaac tgtgagctat cgtcagttga
    24841 tttaagaaga tcacttgttc agatgaaact ggcaggctgg tttgatggca gctcggggat
    24901 catgttcggt agaagtgcgg cagaggattt gttagattat aaaatgaagg atgtatacga
    24961 agatctgtca aaggaattaa aactaccgat cttgtatgat attgattgtg gtcatgtacc
    25021 gccacaactg acattgatta atggtgccta tgcagaagta gcggtagaag atagaaaagc
    25081 gactgttttg caaacgttta tttaaaaaat tttttcctgc cttggagtct atcaaagacc
    25141 aataaatcta aatagaaaag aaagtgaaag aagctgacaa aatgctgtca gcttcttttt
    25201 gtataacata ttagaaagtc taattatcta taactgtcac atcgattaac tgtttcacgt
    25261 cattgggatt tcctgtagga atagctacta cagatgcatt atcaatgatt tcttgagttg
    25321 tttctctaat gaacgctgga taagatgttt gatctgcagg catttgccaa tgtgaaggaa
    25381 ctggataact agtgattgtc ccgtcatggt tacttgaata cactaaaact ccctggtaac
    25441 tgtatttccc agtgagcata accacatctt tcggatagac tgcactccct gcatcataag
    25501 gggcgatagg tgttcctgca ggataatgaa taacatgtaa ttcgaaatca cccgatgtat
    25561 ctctaaaagt attacctcta atagctagcc aaacacgtgc gtactcaatt tgtagatcag
    25621 aatatcctgc taacggatct attgactgag aagactgtga ggaggttgtg tctattgttg
    25681 aagaagaaat atttgtctca actgtactac tagatgtaga agaagtattg ggctctgaga
    25741 ctttactgct tgttgaagat gtattcgttt cagtagaagc tgatttctga gtacttgatt
    25801 tttcagctga actggtttgt ctgattgaac ttttgtctgt ggtcgcagat ggatcactat
    25861 tttctttact aggagacgta catcctacaa ggaataaaga gaccaacaat aaaataaaac
    25921 ctaacttttt catataaaat acctcattat ttttttaata ccagtatagc ataatcctga
    25981 atttttgaat ccgtaaaacc ttgtctatga atataaatag agtgtatctt ctttgataaa
    26041 tgtcagaaaa agtgtgacaa ataatcatca gtaaaattac ttgtcacact attgataagg
    26101 agtatttaat tgaagagaat gattaggaga aaggatctcc gtacttttta tagtaatcat
    26161 agaatccaag tttgtataga cgaatcgttt tttctcttat ttcttctgtt tcaaaatgga
    26221 ctgcttgaag aatcaagtct gtgaattcta taggagctgt tccattcgca gtaatcaaat
    26281 tacgatcttt cacagcttgt tgttctataa aatcttcttt attctgataa ttttccgatt
    26341 ctaaccaagg aaattgcgca tttccggtgt gtttgtaacc ttgcaataat ccgctctgag
    26401 ctaaataatc aactgcccca caaatagcag ctactggtac gtctgttttt aacaatttat
    26461 cgattatttg atataaatga tgatttttta tgtcccatga attgccaccg atcataataa
    26521 aaaggtcaac tttttcaggt atttgttcga ttaagtaatc gatttttgtt tttaatcctc
    26581 caatggaagt gacctctgat tggatagaag cagttttgtt ttgccatttc tcattttggt
    26641 ttagtgtgct agtaatataa gcaccttccc aatcggcata ttcctctaat agaaacgtaa
    26701 tcgcagtttt cattttcaag ctctccttta ctatctataa caaggacaac agttttaacc
    26761 tacagacatt cattaaaatt gggtagcgac agcgaaaaca acaattctta agttttagac
    26821 agagtataca gatagtcaac ttaattaaat acgaactaaa taaaaatgac aggtaatcat
    26881 ttttgtttac cagcattata acattttgca tacatggatg acaaaagaac caattcccac
    26941 ttgcttgagt acaatattaa aagaaactgg aaggcaaaaa gtaattatag aaacgaaaaa
    27001 taaatcatat cgggcactca gacatgaaaa caaccctaga gatatataac taggtaacca
    27061 gtttaatgaa agaaaaaatc atccaagaag tcgattcttg gattttttag tggatttgtt
    27121 cgccaaatac gtttagtatg cgtatatgag aggttgtaag caaagcgttg tggtctgagc
    27181 acttaaggag gaatctgtga aaatagcttt tcatattttt gcagattttg acttaatgcc
    27241 gaaggtcagc tttgcgatta ccgtttatcg agaggctgta aaagcaccgt gtagctccga
    27301 tgacaataag caagcatttt tagagctagg tttccttgat tctaaatatg cggcttattt
    27361 caatgagttg gtgcttgaac gcgattttaa gaaggtcgaa aacaggcacg acttttcgac
    27421 gtaaggagaa agaatttcaa tgatagaaac aataaatccc gcagaaaaca aggtttctgc
    27481 gtacattcat atcccatttt gcgagcatat ctgctattac tgtgatttta ataaagtttt
    27541 tttagaaggg cagccagtag atgagtatgt ggaaatgctg ctgaatgaaa tgaaaataat
    27601 gctagaaagt catccgacag cgaatttaga aacactttac gtagggggcg ggacaccaac
    27661 ttctttatct gctaaacaac ttgatcgact gttatcaggt gctagagaga tcctgccttt
    27721 tcaaaatggt aatgagttta ctgtagaagc gaatccaggt gatttgaccc gtgaaaaact
    27781 tcaagtaatg aaaaactatg gcgtcaatcg tttatccatg ggagtgcaga catttgataa
    27841 tcgcttattg aaaaaaattg gacgtaaaca caccgcagaa gatgtgtatc aaactatgca
    27901 atttttagaa gcagaaaatt ttaccaatgt aagcatcgat ttgatttatg cattaccggg
    27961 acaaacacta gaaggatatc tggatacttt ggaaaaagct ttggctttgg atctgcctca
    28021 ctattctttg tactcattga tcctcgaaaa caaaacaatg ttcatgaact gggtacgaca
    28081 aggaagactc gaactgcctg atcaggaaac ggaaaccaga atgtttgaag agacgattga
    28141 agcaatggaa aagcatggac gtcaccaata tgaaataagt aactttgggc tctccggaca
    28201 tgaatctcaa cataacctca tgtactggaa caatgaccac tattttgggt ttggagctgg
    28261 tgctagcggt tatcttggag atattcgtta ccgcaacaaa ggaccaatcc aacactattt
    28321 acgcccatta cgagcaaatg aacgcccggt attggaagaa gaaaaattga cacgaaaaaa
    28381 tcaaatagaa gaagaaatgt tcttaggact aagaaaaaaa acagggatta ctaaaaaaca
    28441 tttctatgat cgctatcaaa aaacaattga atcggtgtac ggtgatgtgc taagggaatt
    28501 agagcaagaa gggttacttg ttaatgaaga agaccgtgtt tatttgacac caagagggac
    28561 ctttttgggg aacaatgtgt ttgagcgctt tttgtttgaa aaatagttta aagaatattt
    28621 gttttttatc tgaatttaaa taaaaaaagt tcaaaaatac aataaaggac tattgaaacg
    28681 ttgatataac tggatttaca tcaattattt agaattagca ctcttgtgtt tagagtgcta
    28741 aaaaatggag tctttctctt gacaggagaa gggcttcttg ctatatttat aagtggatta
    28801 gcacttagaa tatatgagtg ctaatgagag gtgaggaaga tgttaacaaa gcgacaaaac
    28861 gacattttac gtctgatcat ccaacactat acgagtagtg gcgtgcccgt tggttccaaa
    28921 acattgatgt ctgagggagt caaggccagc tcagcaacga ttcgcaatga tatgaaagca
    28981 ttagaagatg aaggattgct gttgaagact cactcttctt ctgggcggat tccatctgct
    29041 ttaggctatc gttattacgt ggatcatttg ttaaaacctg ctcgcgtagc agaagatgat
    29101 ttgctgctga ttcgtcagtc tctaggcaga gaatttcatg aaatcaatga gattattaag
    29161 caatctgctg agatattatc tgagctgaca agctatacga ctttttcatt gggacctgaa
    29221 ataaaagacc gccgactgac tggctttcgt atcgtaccct tgaataatcg acagattatt
    29281 gctatcgtcg taacagatat ggggaatgta gaaagccaag tgttcactct tcccgaatat
    29341 atgggaagtc aggaactaga aaaaatggta cagattgtaa acgaccgtct agtcggagat
    29401 cctctgatga cggtgtacca taaacttcgt acggagattc cgatgattct tcataaatat
    29461 ttccaaacga cagaagggat gtctcagtta tttgacaccg ttcttggtca tgtttttgaa
    29521 gataaggttt atgtcagtgg acaaatgaat ctcttggatt atgagccccg tcaagatctt
    29581 gatcagttca aatcgatgtt ttcttttatg aaagactcag acggacttac acaaatgatc
    29641 actccgatgg atagcaatat tcatatcaag attggtgctg aattaggcaa tgatttattg
    29701 cagaatatga gtatgattca agcaagttac gagatagatg gtcatggaag aggtacaata
    29761 gccttgttag ggcctactag tatgccttat tctcggatgt tcggtctagt cgatgtttat
    29821 cgaagagaac tagcagctaa acttgctgat tactaccgtt cgcttgactt gtcaaactcg
    29881 taatgtgaat aacgaagcta tttgcagctg atcgttctct ctttcttagt aagagcaatc
    29941 aaacagctga ctgaataatg aaaggaagac tgtatcgtga aagaaaatca agaagaacta
    30001 gataaagaaa tgacagatgc tcagccagaa ccggaaatcg atgtcgaagc tgcagaagat
    30061 tcgggtattt cagaagctga agcggaagaa ttcgaaacag cgaaattaaa agctgagtta
    30121 gaagaaatgg aagataaata cttgcgggct cgtgctgaaa tcgcaaatat ggcaaatcga
    30181 ggaaaaaatg agcgtgagca attacaaaaa taccgttcac aagatttagc caaaaaatta
    30241 cttccatcaa ttgacaacct ggaacgtgca ttagccacag aagtgtcaga tgatcaaggg
    30301 gcaggtttga aaaaaggcgt ggaaatggtc ttggaaagtt tgcgtaacgc actggaagaa
    30361 gaaggaattg aaaaaatccc tgctaaagga gaagcctttg atcctaatct tcatcaagca
    30421 gtacaaactg ttccagcgac tgaagataca ccagccgata cgattgtaga agtactacaa
    30481 gaaggctaca aattacatga tcgcgtgtta agaccaacca tggtcattgt tgcacaataa
    30541 ttgtgcggat ataaaactta cactttcaat taaagaagga gacgttaatt atgagtaaaa
    30601 ttatcggtat tgacttaggt acgactaact ctgcagtagc agtattggaa ggaaacgaag
    30661 caaaaatcat cgctaaccca gaaggaaacc gtacaacacc ttctgttgta tcattcaaaa
    30721 atggagaaat ccaagtaggg gaagttgcaa aacgtcaagc tgtgacaaac ccaaatacga
    30781 tttcatcaat caaacgccat atcggtgaag caggttacaa agtagatgta gaaggtaaat
    30841 catatacacc acaagaaatc tcagcaatga tccttcaata tatcaaagga ttcgcagaag
    30901 actatttagg tgaaaaagtc gaaaaagcag ttatcactgt gccagcttac tttaatgatg
    30961 cacaacgtca agcaacaaaa gatgctggta agatcgctgg tctagaagta gaacgtatcg
    31021 tcaatgaacc aactgcagct gctcttgcct atggtttaga taaaacagat cgcgacgaaa
    31081 aaatcttagt attcgacctt ggtggcggta cttttgacgt atctatcctt gaactaggag
    31141 acggtgtctt tgatgtattg tcaacagctg gcgacaacca tctaggtggg gacgactttg
    31201 ataacaaaat catcgactac atggtagcag aattcaaaaa agaaaacagc attgacttgt
    31261 ctcaagacaa aatggcttta caacgcttga aagacgctgc tgaaaaagcg aaaaaagatt
    31321 tatctggcgt atcaagcaca caaatcagct tgccatttat cacagcagga gaatctggac
    31381 cattgcattt ggaaatgaca ttgacacgtg caaaatttga tgaattaaca gctgacttag
    31441 tagaacgtac aaaagtacca gtacgtcaag cgctaaaaga tgcaggttta tcacaatcag
    31501 aaatcgacga agttatttta gtcggtggat ctactcgtat ccctgcagtt gtagaagcag
    31561 tacgtaaaga aacaggaaaa gaaccaaaca aatcagttaa ccctgatgaa gtagttgcta
    31621 tgggtgctgc tatccaaggt ggcgtgatca ctggtgatgt gaaagatgtt gttttacttg
    31681 acgttacacc attgtcatta ggtatcgaaa caatgggtgg agtgttcact aaattgattg
    31741 accgcaatac aacgattcca acaagtaaat cacaagtatt ctcaactgcg gcagataatc
    31801 aaccagcagt agacatccat gtattacaag gtgaacgccc aatggctgct gacaacaaaa
    31861 ctctaggaag attccaattg acagatattc cagcagctcc tcgtggtatt ccacaaatcg
    31921 aagtaacatt cgatattgat aaaaacggga ttgtaaatgt atctgcgaaa gatctaggta
    31981 cacaaaaaga acaaaaaatc acgatcaaat cttcttcagg tctaacagac gaagaaatcg
    32041 aacgtatggt aaaagatgca gaagcaaatg cagaagcaga taaagctcgt aaagaggaag
    32101 ttgacctacg taatgatgta gatgcattgc tattctctgt ggacaaaaca ttgaaagaat
    32161 tagaaggcaa agtggacgaa gaagaagtga aaaaagcaga aacagctcgc gatgaattga
    32221 aagccgctgt tgaagcaaac aacatcgaag aaatgaaaac taaacgcgat gcattgaacg
    32281 aaatcgttca aaacttgact gttaaactat acgaacaagc ggcacaacaa caagctcaag
    32341 aaaacccaga agcagctcaa ggcggagcag atgatgtcgt tgatgctgat tttgaagaag
    32401 tcgatggcga cgataaataa tcagttcact gaatttcgag agaaggccaa agttgctact
    32461 ttggcttttt cttttgaaaa atagtttgaa gtatcggttt ttgtttcaaa caaaatatga
    32521 tatgataaca agcgacgtga aaatgatgag aaccaatagg aggaaagcct atggcaacaa
    32581 aacgtgatta ttatgaagtc ttagggctct caaaaggagc atcagaagat gaaataaaga
    32641 aagcttatcg taagctttcg aaaaaatacc atcctgacat caataaagaa cctgatgcgg
    32701 aagaaaaatt caaagaagta tcagaagcat atgagatttt aagtgacccg caaaaacgcg
    32761 ctgcatatga ccaatacggg catgcaggca cagatccaaa ttacggtgct ggtggagctg
    32821 gtggcggatt tggtggattc ggcggcttct ctggtggcgg cttcggtggt tttgaagata
    32881 tcttcgactc tttctttgga ggaggcggtc ggtcagtcga tccaaatgcg cctcgtcaag
    32941 gtgctgattt gcaatatacg gtcgacttgt cttttgaaga agcgattttt ggtgtagaaa
    33001 aagaaatcaa gtataatcgg gaagaaatct gtcatacttg tggtgggaac ggtgcaaaac
    33061 caggcacaca accaactact tgtcataaat gtcatggtgc aggtacgatt aatgtagaac
    33121 gccaaacacc acttggacga gtgatgagcc gtcaaacttg tgatgtttgt catgggacag
    33181 ggaaagaaat caaagaacct tgtccaacct gtcatggctc aggacatgag aagaaagcac
    33241 attcagttaa agtgaatgta ccagctggtg tagaagaagg acaacaaatg cgtttagctg
    33301 gtcaaggaga agctggagaa aacggcggac catttggtga tttgtatgtg gtcttccgtg
    33361 tagaagaaag tgatatcttt gatcgtgaag gttctgaaat ttactatgaa ttgcctttaa
    33421 gttttgtaca agcagctttg ggggatgaag tccaagtacc aactgtccat ggagatgtga
    33481 aattgaagat ccctgctgga acacagacag gtacgaactt ccgtttacgt ggaaaaggcg
    33541 ctccgaagtt acgtggtggt gcaacaggcg accaacatgt caaagtaaaa ctgatcacac
    33601 cgaaaaatct aaatgaacaa caaagagaag cattacgtgc attcgctgaa gctggcggca
    33661 tcaaagttga agaacagcaa gaagacggtt tcttcgataa gtttaaagat gcttttggcg
    33721 ggaaaaagcg gaagtagttt ttctagtaaa aacgctaagt cattaataat taaaaaccag
    33781 acgaacaatc atatgttcgc ctggtttttt tgtagttgtc atgtttgtac caacaaaaaa
    33841 gccagcttta gaatatgata atcgtcatag tttctagggg tataaccagt gagagaatgg
    33901 aacttgttca atcgatattg caaggtgttt ttatgaataa ataattcttc tgagcaattt
    33961 ttgatgcttc cgttattttt actgaagcat gccaaagtct tcttgaacaa atgtacttct
    34021 tcttctgtca ggcttttcaa aatacgtttt acatataatg ttcgttgatc tgacgggatt
    34081 gctggtaaaa cgatcccgag gtctaaatct tcaaaaaagc ggatctcgtt cacagattga
    34141 tggaaagcta tccattcgca aacttgtttg gcatgaagga aagattgcgg tgtctcttgc
    34201 tctgtataac aaagcgaccc aattccaaag tttagctgta actgatacat tttattaaac
    34261 tttgtctgga ttttatcaag caatgatagc aatgaggctc tggaaatccg attgactagg
    34321 ataatgattt gttgtctatg tacagtgagc aaagcctgtc cgtaataaga gaagaccctt
    34381 tcgagcaact ccaataaagg attgatccgt tcttgacaca attgctgatt gtcagaaagt
    34441 tgtcccacaa ctacataata tggagaagaa aaatcaagct caattaagtg agggtggtat
    34501 cgactatgta cttgatatca tcaatgaacc gatggtactt tcttatgcga tggaacaggt
    34561 agagcgattg cttgtttgtg caggaaaaca agcgatgcgg ctgatacgat taggtgagaa
    34621 aataagaaga taaaaataca cggtcccttc atggcgaccg tgcatttttg gtttttatta
    34681 aatatttaat actttattca agaaatcttt tgtccgttca ttttgcggat tttcaaagat
    34741 ttgttgaggc gttccatctt ctaaaaagtt tccgtcgtca atgaacatga cgcgattggc
    34801 cacttcttta gcgaacccca tttcgtgcgt aacgataacc atcgtcatac cttgttctgc
    34861 taactttttc atgacgttca agacatcacc gaccatttca ggatcgagtg cagaagtagg
    34921 ctcatcaaaa agcatgatgt cagggttcat cgctagagca cgagcaatag ccacacgctg
    34981 tttttgtcca ccagaaagca tttcaggata catatctttt ttatcttcta gaccaactgt
    35041 ctgcagtaat ttgattgctt ttttctctgc ttcttctcta gattctttct tcaggtccat
    35101 cggtgctaaa atgatgtttt ccaaaataga aagatgaggg aataggttaa agtgttggaa
    35161 taccattccg atatgttgtc tcaccaagtt gatatctgtg cttttatcca tcaagtgggc
    35221 gccgtcaata ataatttcac cgtcagaagg ttcttctaaa cgattcaagc aacgcaagaa
    35281 tgtacttttt cctgaacctg aagggccaat gatacagacg acatctcctt cgttgatgga
    35341 tacattgatg tcgtttaata ctgtgttatc gccgtatttt ttgactaaat gctcaactaa
    35401 gattttttct gccatcttac ttcaccttct tttctaaaac ttttgctaat tttgtcaatg
    35461 ctgtgatcaa aatcaagtac atgatcgcaa tgatcaagta aacatacgta ctttgtgttg
    35521 tacgtgcaac aatgatctta ccagtttgca gcaattcgac tacaccgatt gcggaaatga
    35581 tcgttgtgtc ttttaatgag ataacgaact gattgacgaa tgatgggatc atgatgcgta
    35641 tcgcttgcgg caaaatgatt ttttgcattg tccgattata agaaagacct aaacttctgg
    35701 aagcttccat ttgtccgaca ggaacggcgt tgatcccgcc tcgaacaatc tctgcgatat
    35761 aagcacttgc gttaagagtc aaggtgataa caccagcaag gaagtcagga atcgtaaatc
    35821 caacgatacc aggcaaccca aagaaaataa agaacgcaag aaccatcatt ggaattcctc
    35881 ggatgatatc gacataaata ctagcaattg tgcgaagagc gcgtattggt gctacactga
    35941 ataatccaaa aatcacaccg attactatag ccaaagcaaa ggagatcaaa gttaggagaa
    36001 ttgttctcca aagtcctttc aacaatgctt gatagttgtt ttggataagt ccaagaaatg
    36061 ttgattcatc agcagtggaa gactcactgg aagtttctga accatccgct acatattctt
    36121 taataatctt atcgtattcg cctgttcttt ttaattcttt caatccttca ttgaacatat
    36181 taagaagctc aggattttgt ccttttttaa cggcaaatgc atattttccg ccagattctt
    36241 ttttgatcgg tgtttccaat tcttgtcctt gtttgatcgc ataaccgatt accggataat
    36301 catccatcat tgcatcgatt gcgccaactt ttaacccgtc gtacaattga tcagcagcat
    36361 caaaatattt gatagaatag ccatattcag attcatggtc gcttaaaaat tcagcacttt
    36421 cggtaccaac ttttgctccg actgtttttc ctttcaaatc ttcataagac ttgatcgaag
    36481 tatctccttt tttgacagct aattggatcc cgctatcaaa gtaagaatca gaaaaatcat
    36541 agcttttctt tcgttcatct gttacagtca ttccggcaat cattccgtct gcttgtcctg
    36601 attccaaagc ttgcaaagca gcagagaaac cacggtagtc cattcggatg ttaaatcctt
    36661 gcagtttggc gatgttttcc atcaatttta catcgatgcc ttcgtactcg ccctttgcgt
    36721 tttgaaattc aaatggagca aaagcagagt cactggcaat tacgtatacg tcttttttgg
    36781 gctctatttt ttgcattgtc tcgcttgtgc ttgactctgc cgttgtagtg ttgtcagctt
    36841 gtgcaacatg accaaacgca aatgtcacaa gtcctgtcat taagaaaaca aatgagtgaa
    36901 ataaatgctt tcttttcata atcaacctcc taaatttcca atattttatt attataggcg
    36961 aaaagtgctg aatcagacaa gactgtaagc tcgaaaaaat gaaaaaaatg cgagtttcta
    37021 acattttatg ttataaaaca taacatgtat aataagataa aaaagtggcg aaagtatgtt
    37081 cggtgtggta taatttttta gtaaggaatg cctttgcttt ccttttcttt ttattggtcg
    37141 taaaatgaaa aggaatgaat gaaaaggagg acgaagaatg attgagagag atacctcccg
    37201 acactttgag cttgtctcga aataccaacc ggctggtgat cagcctgaag cgattaacca
    37261 attagtggat ggcgtagtcg gcgggaaaaa agcacaaatc ttattaggtg cgactggaac
    37321 agggaaaaca tatacgattt ccaatcttat tgagaaagtg aataaaccaa cactgattat
    37381 tgcccataac aaaacattgg ctggtcagct gtacggggaa ttcaaggaat ttttccctaa
    37441 caatgctgtt gaatattttg tttcttatta tgattattat cagcctgaag cttatgtgcc
    37501 ttcaagcgat acgtatatcg aaaaagattc aagtgtaaat gatgagatcg acaaattacg
    37561 acattcggct actagttcat tattagaaag aaatgatgtg atcgtcattg catccgtttc
    37621 atgtattttt ggattaggct cgccttttga gtaccaaaaa caagtagttt cgattcgaca
    37681 gggagcagaa ctagatcgca atcagttgat tcgtgactta gtcagtatcc aattcgaaag
    37741 aaatgatata gattttcagc gcggtcgttt tcgagtgcgc ggtgatgtcg tggagatttt
    37801 tccggcttca agagatgaac gtgccttacg tgtggagttt tttggagatg aggtcgaacg
    37861 tatccgagaa gtcaatgcac tgacaggtga agttttagga gaaacggaac acgtagcaat
    37921 cttcccggcg actcactttg tgacgaatga tgaacatatg gagcatgccg tagcgaatat
    37981 caaagcggaa ttagaacagc gtttaactgt cttgcgaaat gaaaacaaac ttctggaagc
    38041 tcaacgttta gaacagcgaa ccaattacga tattgaaatg atgttagaaa tgggctacac
    38101 gtcgggaatt gaaaattatt ctcggcatat ggacggacgt aaagaaggtg aaccgcctta
    38161 tacgctattg gatttctttc ctgaagattt tttgatcgtg gcggacgaat cgcacgtaac
    38221 catgcctcaa atcagaggaa tgtataatgg tgaccgagcg agaaaacaga tgctagtaga
    38281 ttacggtttc cgtttgccat ctgctttaga caatcggcca cttcggttag aagagtttga
    38341 aaaacatgtc aatcagatca tttatgtctc tgcgacgcca ggaccatatg aacatgaaca
    38401 aacagatacc gttatccaac aaatcatccg tccgacaggg ctgcttgacc cagtgatcga
    38461 agttcgtcca atcatgggac aaatcgatga tctagtaggc gaaataaatg aacgagtcga
    38521 aaaagaccag cgggtgtttg taacgacatt gacgaaaaaa atggcagaag acctcacaga
    38581 ttatttcaaa gaattaggga tcaaagtcaa atatttgcat agtgatatca aaacgctgga
    38641 acgaacagaa atcattcgag atcttcgatt aggtgaattt gatgtgttag ttggaatcaa
    38701 tttgcttcgt gaaggattag acgtacctga agtttccctt gttgctatct tggatgcgga
    38761 taaagaaggc tttttacgta gtgaacgttc cttagtccaa acgattggac gagctgctcg
    38821 gaatgaagaa ggtaaagtaa tcatgtacgc agacaaagta acagattcta tgcgtctagc
    38881 gatggatgaa acgtcaagac gtcgtacgat tcagcagaag tacaatgaag aacatgggat
    38941 cgttccaaaa acgatcatta aagaaatccg agatttgatt tctattacga aagaatcgga
    39001 agatgataca aaagaagcgg tacaagtctc gtacgaagaa atgacgaagg aagaaaaaga
    39061 cacgttattg atgaaattag aaaaagaaat gaaagatgcg gcaaaagctt tggattttga
    39121 aacagccgcg aatgtgcgag atatgatttt agaattaaaa gcttcaaagt aggaggacaa
    39181 catggctaac gataaaatta cgattcatgg tgcacgtgcc cataacttga aaaatataga
    39241 tgtaacgatt ccacgagata aattcgtggt cgttacaggt ttgtccggtt ctggaaaaag
    39301 ttccctagca tttgatacgc tgtacgctga agggcagcgg agatacgtag agagcctttc
    39361 tgcttatgct cgtcagtttt taggacaaat ggataagccg gacgtagata gcatcgacgg
    39421 attgagtccg gcgatttcta ttgatcagaa aacaacaagt aaaaatcctc ggtcgacagt
    39481 aggtactgtg acggaaatca atgactattt acgcttgttg tttgcacgtg taggacatcc
    39541 gatttgtccc aacgatcata tcgagattac cagtcaatca gtagatcaaa tggcagatca
    39601 agtaatggct ttgcctgagc gcacaaagat ccaagtgttg gcaccgatcg tggtaaagaa
    39661 aaaaggacaa cataaaaaaa tattcgaacg aatccaaaaa gagggatacg tccgcgttcg
    39721 agtcgatggt gagatctatg atttaagtga agtgcctgag ctggagaaaa acaaaaaaca
    39781 tgatatcgcg atcgtcatcg accggatcat tgtgaaagaa gggattcgtt ctcgcctatt
    39841 tgattcttta gaggcggccc tgcgattagc agacggctat gctctggttg acgtgatcgg
    39901 tgaagaagaa atgttgttta gcgaacacta tgcctgtcct tattgcggat ttactgttgg
    39961 cgaactagaa ccaagactgt tctcattcaa tgcgcctttt ggagcttgtc cagactgtga
    40021 tggtttaggt gtcaaattag aagtcgatca agatttagtg attccagata gaacgaagac
    40081 acttagtcaa ggtgctatcg ttccatggaa tccaatcagc tctcagtatt atcctcaaat
    40141 gttagcgcag gcttgtgaaa gtttcggtat cgatttagat acaccattcg aagaattacc
    40201 ggaagagcat caggagatca tcttgaatgg gacagatgat cttttccatt tccattatga
    40261 aaatgatttt ggtggcgttc gcgatgtaga aacgactttc gaaggggtat tgaagaatat
    40321 cgagcgtcgt tatcatgata cgaacagtga tttcacgcgt gagcaaatgc gtctttatat
    40381 gacagaatta acttgtcaga catgtaaagg ttatcgattg aatccccaag ctctatctgt
    40441 tcaaatcaat ggaacgaata tcgggcaaac aaatgagttg tctattcaaa aagcagtaga
    40501 tttcttctca aacttaacgt tatctgaaca agagaaagta attgccaaac cgattttaaa
    40561 agaagtaaat gacagacttt cattcttgga aaatgtggga ctagactatc taactttgag
    40621 tcgcgcttcc ggaacattat ctgggggaga agcgcagcga atcagattgg ctacacaaat
    40681 tggatcgaat ctttctggag ttttgtatat tttagatgaa ccatcgatcg gattgcatca
    40741 acgcgacaac gatcgactga ttggctcatt aaagaaaatg cgtgatcttg gcaacaccct
    40801 cgttgtagtc gaacatgatg aagatacgat gtgggcagca gattatttga ttgacgtcgg
    40861 acctggggcc ggagaacaag gcggacaaat cgtggctgca ggcactcctg aagaagtagc
    40921 taatgatgaa aattctttga cagggaaata tctttcaggg aaaaaatcaa tccctgtacc
    40981 aaaagaacgc cgtaaaggta atgggaaagc aatcaagatc actggtgctt cagaaaacaa
    41041 tctgaaaaat attagtgttg aatttccatt aggagaattt gtagcagtca caggtgtgtc
    41101 tggttctgga aagagtacat tggtcaatag tatcttaaag aaatcgttag cgcaaaaatt
    41161 aaataagaat tctgctaagc caggtaaatt caagacaatt tccggctacg aaagtatcga
    41221 aaagatcatc gatatcgatc aaagcccaat cggccggacg ccgagaagta atccagcgac
    41281 ttatacaagt gtatttgatg atatccgtgg gttatttgct caaacgaacg aggcaaaaat
    41341 gcggggttat aagaaagggc gttttagttt caacgtaaaa ggcggtcgtt gtgaagcttg
    41401 tcgcggggat ggaattatta agatcgaaat gcactttttg cctgatgtct atgttccttg
    41461 tgaagtatgt catggtaaac gatataattc tgaaacatta gaagtgcatt acaaaggaaa
    41521 aagcattgct gatattttgg aaatgacagt agaggatgct gtagaattct tcaagcacat
    41581 tccaaagatt catcgcaaac tgcaaacgat tgttgatgtt ggcttaggtt atgtgacgat
    41641 ggggcaacca gcaacgacat tgtccggtgg tgaggcacaa cggatgaaac ttgccagtga
    41701 attgcacaaa atctctaatg gaaagaattt ctatatacta gatgaaccaa cgacaggact
    41761 tcatagcgat gacatcgccc gcttgttgca tgtattacaa agattagtag atgctggtaa
    41821 cacagtttta gtgattgaac acaatctaga tgtaatcaaa acagcagatt atatcattga
    41881 tttaggacca gaaggtggag aaggtggagg aacgatcctt gcgactggaa caccagaaga
    41941 aatcattaac gtaaaagaaa gttatacagg tcactatttg aaaaaaataa tggtataaag
    42001 aggatagata gctgagatag ttaaaaaaac ataaaaaaat gaatttttgc ttgttttatt
    42061 aatatcagtg ttctagaatc attacatggt agttctttct acactgattg gtatagataa
    42121 aaacttccta taagttcaat ccgttcaaaa aagaattgag caatcggagg actttccttt
    42181 gattgctttt ttctttatgg tatgatattc cttgtgtagt tggaaaagaa aaacaagaga
    42241 ttatgagtaa gtgagagata atgatgaaca gtattgaaaa tgaagaattt atccctatat
    42301 tattaggaag cgacatgaac gtttatggta tggcacgttc ttttaatgaa gcatacggca
    42361 aaatctgcca agcatatgct tcggatcagt tagcccctac ccgctacagt aaaattgtca
    42421 atgtagaagt tattccagga tttgataaag accctgtatt tatcgagacg atgcttcgtt
    42481 tggcaaaaga aagatattct gataagagta aaaaatatct tttgattgct tgtggtgatg
    42541 gatatgcaga attgatctcc caacataaac aagagctgtc agaatatttt atctgtccat
    42601 atattgatta ttctttattt gaaaggctga tcaataaagt aagcttttat gaagtttgtg
    42661 aagaatatga tcttccgtat cctaaaacat tgatcgtcag agaagaaatg ttagtaaatg
    42721 gacatttgga acaggaattg ccttttgaat tcccagttgc tttgaaacct gcaaacagtg
    42781 tggagtatct ttctgtccaa tttgaaggaa gaaaaaaagc ttttatttta gagactagag
    42841 aagagttcga tttaatttta ggccggatat acgaagccgg ttataaaagt gaaatgattg
    42901 tacaagattt tatcccgggt gatgatagta atatgcgtgt gctaaatgcg tatgtagatg
    42961 aagatcatca agttcggatg atgtgccttg gtcatccact attagaagat cctacaccag
    43021 catcgattgg taattacgtt gtgatcatgc cagattataa cgaaaaaatc tatcaaacca
    43081 ttaaggcatt tttggaaaaa atcgaatata caggctttgc caatttcgat atgaagtatg
    43141 atcctagaga tggagaatat aaattatttg aaatcaattt gagacaaggt cgtagcagtt
    43201 tctttgtgac attgaacggc ttgaatctgg ctcgttttgt cacagaagat cgagtgttca
    43261 acaaaccctt tgttgaaacg acctatggaa cgaatcaatc agataaagca agattatgga
    43321 tgggtgtgcc caaaaaaatc tttttagaat atgctcggga aaatgaagac aaaaaactag
    43381 ctgagcaaat gatcaaagaa aatcgttacg gtactactgt attttatgaa aaagaccgat
    43441 caatcaagcg ctggttgttg atgaaatata tgttccataa ctatattcca agattcaaaa
    43501 agtattttca tgtgaaagaa ggctagttca tggagaattt tttcagtatt ttaggcggaa
    43561 tgggcacgat ggcgacagaa agttttgttc gcttgatcaa ccaccgaacg aaagctacaa
    43621 aagatcaaga atatttgaat tatgtcttat tcaatcatgc aacagttcct gatcgtacag
    43681 cttatatttt agatcgatca gaagaaaatc caatgccgtt tctgctggat gatattgaga
    43741 aacaaaattt attgcgacca aacttcattg tgttgacttg taacaccgca cactactttt
    43801 ttgaagagtt acaagcggca acggatattc ctatcttgca tatgccaaga gaagcagcaa
    43861 atgaacttgt ccgtcagcat acgacaggga gagtagcgat tctaggaaca gaaggaagta
    43921 tgaaagctgg aatctatgaa cgcgaagtca aaaatcttgg tttcgaaaca gtgattcctg
    43981 atactgcgct tcaagaaaaa atcaattacc tgatctatca tgagatcaag gaatcggatt
    44041 atttgaatca agagctctat tacgaaatct tagaggaagc ggtagagcgc ctaaactgtg
    44101 aaaaagtcat cctaggttgc acagaattat ctttgatgca tgaatttgct gaagataatc
    44161 attacccagt gatcgatgct caatctattt tagcggatcg aacgatcgaa cgtgcattag
    44221 ctgaaagaag cgaggcattg gatacagcat cggaaaagta aaaaaattgg atttggggca
    44281 aatatcctaa aagatatttg gtccaaatct ttttgtaaaa gaaaaagaaa actggtaatg
    44341 atacgcccgg gatgatataa ttttattatg cacaatgtga gaggggttcg taaatatgac
    44401 agataatttg caattagtca tcataacagg tatgagtggt gcagggaaaa cagtagctat
    44461 ccaaagtttt gaagatatgg gctatttttg tatcgataat atgccgcctc gattgattcc
    44521 aaaattctgg gaattgatca aagaatcagg taaagtcaca aaaatggctt taggagtgga
    44581 tttgcgttct cgttccttct ttgaagaaat ccaaaacatg ctgatcgaaa tcgaaaatac
    44641 gaacttcatc gacacttcta tcttattttt agatgcttca gacgaagaac tagtggctcg
    44701 ctacaaagaa acaagacgag cacatccaat ggcgatggac ggtttgatca ctgaagggat
    44761 acgtaaagaa cgggcgatac tagacgactt gaaagcccaa gcttccgtga tcattgatac
    44821 gacaacattg acaccacgac aattacgtga aaaaatcaat gagtcattca aatcaaaggg
    44881 agaccaagga tttcggatcg aatttgtatc atttggtttt aaatacgggt tgccgatcga
    44941 ttcggatatt gtgatggatg tgcgtttttt gcccaaccct cattatatcc cggaactaag
    45001 accattgacc ggaaaagatc agccagtcta tgattatgtg atgtcctttg atgaaacgca
    45061 atcattctac cataaatttt tggatcttct ggaaactgta ttgcctggat acattaaaga
    45121 aggaaaaagc agcttgacag ttgcaatcgg ctgtacaggc ggtcaacacc gttctgtggc
    45181 attgactgaa cgtttggcaa atgctttagg gaaaaactat aaagtcaatg tgacacatcg
    45241 ggacaaggat aaaaggaaag agacggtgaa ccggtcgtga aaatgaaaac ctaccggata
    45301 agaaaaccaa agatcgtagt ggtcggcggt ggcactggtc tgccagttat cctgaaaagt
    45361 ttacgaaatc aaggagcaga tatcacagca gtagtgactg ttgcagatga tggtggaagc
    45421 agcggtgcaa tcagagaatc gatcgctatg gctcctcctg gtgacttgag aaatgtctta
    45481 gtggcgttat cggatatgcc acaattctat gaagatattt tccaataccg tttcaaaaag
    45541 gaagatcagt ttttagccaa tcatacgatc ggtaatctga tcattgccgc agtttccgag
    45601 atgcggggaa gcacttatga ggcgattcaa ttattagcaa aaatgatgca tgtagatgga
    45661 catgtttatc cttcttcaga aactcctttg accttgcatg ctgtttttaa agacggaacg
    45721 actgcagtag gagaatctaa aatcgcgatc aaccgtaaaa ccattgaccg tgtgtatgtc
    45781 tgcaacacaa atgataaaag tgaagccaaa gcggctcgaa aagtcgtttc agcaattatg
    45841 gacgccgaca tggtcgtttt gggacccgga agtttattca ccagtatcct tccaaattta
    45901 gtgataccag aaatcggcga agcaatgctc aagacaactg ctgaaaccgt ctatatctgt
    45961 aatatcatga cccaaaaagg ggaaacggaa catttcacag atgccgatca tatacgtgtt
    46021 ttacacaagc atttgaatca gccttttatc gatacagtat tggtcaatac ggaaaaagtg
    46081 cctgaagatt atatggaccc agagatctat gatgagtatc tagtacaagt cgcacacgac
    46141 ttcaacggat tgagaaatga aggatgtcga gtgatttcca ccgacttttt agaattgaaa
    46201 gatggtggtg tcttccacga tggagacaaa gtagtagaag aattgttccg tttagttttc
    46261 ggttcaaaat attaagcaat ttattgttgg agaaaggagg agaataggat gtcatttgct
    46321 gcagatgtga agaaagaact aactggtttg gaagttcatc gagaacatgc gaaagcggaa
    46381 ttagctgctt tgctgcgaat gaacggctct ttaggtattg tgaaccagca gtttattttg
    46441 aacgtccaaa cagaaaatgc tgcaattgcc agacgtatct attctctttt gaaagatcat
    46501 tatcaggtac gaagtgagct actcgttcgt aaaaaaatga aattaaaaaa gaacaatgtc
    46561 tatatcgtcc ggttgaagca aggcacaaaa gatgtgttga tggatcttga tatcatggat
    46621 ggtatgatgt tcaatggaca cgtttcggac gagattatgg gaaattcgca aaaaatgcgt
    46681 tcttacttac gtggtgcttt tatggcctca ggttcagtca ataatccgga aacgagtcgt
    46741 tatcatttgg aaatattttc tatttatgaa cagcataacc aagacatttg cgatatgtta
    46801 aattattacg gtctacatgc tcgaacgctt gaacggcgca atggctatat ttcgtattta
    46861 aaaggagcag aaaaaattgc tgatttctta acattgattg gtgcaaccaa ctcaatgctc
    46921 cgatttgaag atgttcgaat cgtacgggat atgagaaatt cggtcaatcg tctagtcaat
    46981 tgtgagacag caaatctgaa caagacgatt gatgcggctt ctaaacaaat cgaaaacatc
    47041 gaattgatcg aagcaaaggt cggtctacat gcccttcctg aaaaacttca ggaaatcgca
    47101 gaacttcggc ttcaacatcc agaggtcagc ttgaaagaat taggggaaat gattccctca
    47161 ggtgcaatat caaaatcggg aattaatcac cgaattcgca aaataaatga atttgcggaa
    47221 aaactgcgtg aagaagcagt ttaatccaaa gaaaaaactg atacatgaag caggagatgc
    47281 gaatgtatca gtttttttta aaagctatac ttgcttttct tccgtcaaac tataattcca
    47341 actttactgg agtaacataa tgttgaaagg cttctattgt tttttaatac aatcgtataa
    47401 aaagcgggga gacaactgaa tgaagatttt agttttatac ataaactttt gcttaatata
    47461 gaagtcctga tatagatagg atcaagtatt tatctataac tggagattaa ttaaaaactg
    47521 gaaggaactg ctcgatcaac cttgagaact gtttttctta cttctggtca agattctcgt
    47581 ggttggtgga taaggatggt tgataagttc attggattag ctagtcgaaa acaaccaggt
    47641 gtaattaatg tagaagtttt aacaagggga tggagtgatt tattcagtga taatcataag
    47701 ttcataaata gtaattggga tgtatggttg gtatacgtta tacctgaact tgaactatca
    47761 agatttggtt tagaatttga gaatcgatgt ttataaaggg agggcaaaaa atgtgctttt
    47821 ttacaacttt tactatcatt atgattgcag tactttttat ttttctagat atagcaaagc
    47881 gaaatacagc atttttatta tatcgggttt tacttcgagc aggtctcata acatttatat
    47941 cgatcgttgg attttttcta ttcacagtta ttgtatttat ttggagaact acagcaccac
    48001 cattgccaga gattacatat ggagaatttc cgtttagtct tgaatatgaa atagatgagg
    48061 aaagaatttt gattgaagat acggtgattg cagaatttat aggaagtttt agagggaatg
    48121 tgacaactca acctagacga agttggagta taaaactttt aaataatagt ggagagttga
    48181 aagcatttca ttatgaattc ttaataatag aaagtgaaga tgtaacaatc actttaagta
    48241 gtggctctgc tggatatttt atgaatgatg gtggaagcaa gccacagctc acagtccgtg
    48301 atcgtattgc aggttttatg catttttctc actatgactt agaagaagct cgtaaaatac
    48361 ttgcagaata tgggatcaca ttaatcaatt gggaatatgc gccaccgata agaaattttt
    48421 tcaatgatta ttattagtct tataaaaaac agaatgtaaa atttatgtgc gcgagtattt
    48481 agttttccat tttttgtttg atagatatat agcattgttg aagcatggga tagtggtgcc
    48541 aattttatca atgaggttgg tcaggcagtt aacataattg gtgatgctat aaatccattt
    48601 aattggaatt ggaggaatta ggtgtggaaa cagtgagatt atggatattc atgaaaaatt
    48661 ttcgttcggc tttgagatat gttattctgg aatcaaaatc aggttattat ctgattgaca
    48721 cagatcacta tactttttgg ggaggctacc tttttccgat ttttaattgg ataatccccc
    48781 ataaggttat gaaaattaac ttgcctgcag aagaaataaa tagattaata ctcaacaaag
    48841 atttacaaag gaaaagagag aaagaacatt ctaatgttgc ttggataggt ccagcatttg
    48901 cagtagtgac ttcttcaatt gtcggtccta tgctagagag tatttatagt gatttttcta
    48961 ttggaatacg ttatttattt gttactttaa gtatagctgt tcttttgtgc ataaagggtt
    49021 atgttagcaa gaagaataaa gatattttaa cactaatcgg tgtaaacaat ttatctagta
    49081 ctaaaattat actgtttcct gaatctataa aagaaggatc aaggttgttg ataattttta
    49141 taatgcttat actgttttct gttttatttg gggtgatgtt tata
//
LOCUS       DARBFO010000012        43369 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000012 DARBFO010000000
VERSION     DARBFO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.012"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(8..2389)
                     /locus_tag="SCK51_000821"
     CDS             complement(8..2389)
                     /locus_tag="SCK51_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEG1251466.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2379..3026)
                     /gene="recU"
                     /locus_tag="SCK51_000822"
     CDS             complement(2379..3026)
                     /gene="recU"
                     /locus_tag="SCK51_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEG1251467.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3090..3629
                     /locus_tag="SCK51_000823"
     CDS             3090..3629
                     /locus_tag="SCK51_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEG1251468.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3719..4138
                     /gene="gpsB"
                     /locus_tag="SCK51_000824"
     CDS             3719..4138
                     /gene="gpsB"
                     /locus_tag="SCK51_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEG1251469.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4160..4527
                     /gene="rnpB"
                     /locus_tag="SCK51_000825"
     ncRNA           4160..4527
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="SCK51_000825"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4736..5902
                     /locus_tag="SCK51_000826"
     CDS             4736..5902
                     /locus_tag="SCK51_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEG1251470.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            5948..7444
                     /locus_tag="SCK51_000827"
     CDS             5948..7444
                     /locus_tag="SCK51_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEG1251471.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7525..8208
                     /locus_tag="SCK51_000828"
     CDS             7525..8208
                     /locus_tag="SCK51_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1251472.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8853..10043
                     /locus_tag="SCK51_000829"
     CDS             8853..10043
                     /locus_tag="SCK51_000829"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEG1251473.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10145..11098
                     /locus_tag="SCK51_000830"
     CDS             10145..11098
                     /locus_tag="SCK51_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEG1251474.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11167..12516
                     /locus_tag="SCK51_000831"
     CDS             11167..12516
                     /locus_tag="SCK51_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1251475.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12699..13022)
                     /locus_tag="SCK51_000832"
     CDS             complement(12699..13022)
                     /locus_tag="SCK51_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251476.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13151..14314
                     /locus_tag="SCK51_000833"
     CDS             13151..14314
                     /locus_tag="SCK51_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG1251477.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14384..15733
                     /locus_tag="SCK51_000834"
     CDS             14384..15733
                     /locus_tag="SCK51_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG1251478.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15778..16209)
                     /locus_tag="SCK51_000835"
     CDS             complement(15778..16209)
                     /locus_tag="SCK51_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1251479.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16597..19239
                     /gene="alaS"
                     /locus_tag="SCK51_000836"
     CDS             16597..19239
                     /gene="alaS"
                     /locus_tag="SCK51_000836"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEG1251480.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19439..20035
                     /locus_tag="SCK51_000837"
     CDS             19439..20035
                     /locus_tag="SCK51_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1251481.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20157..21305
                     /locus_tag="SCK51_000838"
     CDS             20157..21305
                     /locus_tag="SCK51_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG1251482.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21343..21756)
                     /locus_tag="SCK51_000839"
     CDS             complement(21343..21756)
                     /locus_tag="SCK51_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEG1251483.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            21835..22266
                     /locus_tag="SCK51_000840"
     CDS             21835..22266
                     /locus_tag="SCK51_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEG1251484.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22365..22598)
                     /locus_tag="SCK51_000841"
     CDS             complement(22365..22598)
                     /locus_tag="SCK51_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEG1251485.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22781..24451
                     /locus_tag="SCK51_000842"
     CDS             22781..24451
                     /locus_tag="SCK51_000842"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEG1251486.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24598..24984
                     /locus_tag="SCK51_000843"
     CDS             24598..24984
                     /locus_tag="SCK51_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251487.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            24971..25669
                     /locus_tag="SCK51_000844"
     CDS             24971..25669
                     /locus_tag="SCK51_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1251488.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25674..26138
                     /locus_tag="SCK51_000845"
     CDS             25674..26138
                     /locus_tag="SCK51_000845"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEG1251489.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26135..27046
                     /locus_tag="SCK51_000846"
     CDS             26135..27046
                     /locus_tag="SCK51_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG1251490.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27281..27820
                     /gene="pyrR"
                     /locus_tag="SCK51_000847"
     CDS             27281..27820
                     /gene="pyrR"
                     /locus_tag="SCK51_000847"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEG1251491.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            27834..29117
                     /locus_tag="SCK51_000848"
     CDS             27834..29117
                     /locus_tag="SCK51_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEG1251492.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29130..30056
                     /locus_tag="SCK51_000849"
     CDS             29130..30056
                     /locus_tag="SCK51_000849"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEG1251493.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30075..31355
                     /locus_tag="SCK51_000850"
     CDS             30075..31355
                     /locus_tag="SCK51_000850"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEG1251494.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31360..32436
                     /gene="carA"
                     /locus_tag="SCK51_000851"
     CDS             31360..32436
                     /gene="carA"
                     /locus_tag="SCK51_000851"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEG1251495.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32441..35626
                     /gene="carB"
                     /locus_tag="SCK51_000852"
     CDS             32441..35626
                     /gene="carB"
                     /locus_tag="SCK51_000852"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEG1251496.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35680..36456
                     /locus_tag="SCK51_000853"
     CDS             35680..36456
                     /locus_tag="SCK51_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEG1251497.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36453..37385
                     /locus_tag="SCK51_000854"
     CDS             36453..37385
                     /locus_tag="SCK51_000854"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEG1251498.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37382..38098
                     /gene="pyrF"
                     /locus_tag="SCK51_000855"
     CDS             37382..38098
                     /gene="pyrF"
                     /locus_tag="SCK51_000855"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEG1251499.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38105..38794
                     /locus_tag="SCK51_000856"
     CDS             38105..38794
                     /locus_tag="SCK51_000856"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEG1251500.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            38767..39036
                     /locus_tag="SCK51_000857"
     CDS             38767..39036
                     /locus_tag="SCK51_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251501.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39074..39715
                     /locus_tag="SCK51_000858"
     CDS             39074..39715
                     /locus_tag="SCK51_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1251502.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            39758..40450
                     /locus_tag="SCK51_000859"
     CDS             39758..40450
                     /locus_tag="SCK51_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEG1251503.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40447..41349
                     /locus_tag="SCK51_000860"
     CDS             40447..41349
                     /locus_tag="SCK51_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1251504.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41507..43213)
                     /gene="efbA"
                     /locus_tag="SCK51_000861"
     CDS             complement(41507..43213)
                     /gene="efbA"
                     /locus_tag="SCK51_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEG1251505.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13875 a         7663 c         9440 g        12391 t
ORIGIN      
        1 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
       61 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      121 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      181 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      241 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      301 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
      361 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
      421 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
      481 tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
      541 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
      601 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
      661 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
      721 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
      781 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
      841 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
      901 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
      961 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1021 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1081 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1141 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1201 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1261 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1321 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     1381 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     1441 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     1501 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     1561 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     1621 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     1681 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     1741 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     1801 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     1861 gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     1921 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     1981 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2041 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2101 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2161 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2221 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2281 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     2341 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     2401 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     2461 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     2521 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     2581 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     2641 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     2701 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     2761 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     2821 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     2881 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     2941 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3001 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3061 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3121 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3181 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3241 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3301 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactgga
     3361 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     3421 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     3481 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     3541 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     3601 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     3661 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     3721 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     3781 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaagc
     3841 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     3901 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     3961 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4021 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4081 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4141 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4201 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4261 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4321 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     4381 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     4441 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     4501 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     4561 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     4621 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     4681 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     4741 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     4801 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     4861 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     4921 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     4981 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5041 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5101 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5161 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5221 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5281 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     5341 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     5401 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     5461 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     5521 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     5581 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     5641 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     5701 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     5761 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     5821 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     5881 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     5941 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6001 agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6061 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6121 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6181 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6241 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6301 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     6361 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     6421 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     6481 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     6541 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     6601 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     6661 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     6721 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     6781 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     6841 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     6901 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     6961 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7021 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7081 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7141 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7201 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7261 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7321 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     7381 taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat atcaggtaaa
     7441 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     7501 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     7561 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     7621 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     7681 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     7741 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     7801 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     7861 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     7921 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     7981 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8041 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8101 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8161 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8221 cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac ttctggaaca
     8281 ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     8341 aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     8401 accgtttatt cggaagaata ggactgtgac aagacttttg tcacagtcct tttttcgaat
     8461 tcaacaagta aaacttttaa gaaatattca gagagtaaaa ttaaaaattg ctgataaacg
     8521 aagatggaaa ttttatgtat ttttattggt ctatgcaata aataaattaa atattcagaa
     8581 tacaattgat tttaacgaaa gggcttctat caatggttgt gttgtattta tctgcccagg
     8641 tagagtaaaa tagatcatta tctaaaaggt aattgttcat actatataag tcatttgaaa
     8701 gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt ctcataatca gtgtaagcgt
     8761 tatcaaaaat atagcatact tgctttgtag atgaatgaca cgatatatgt ttggtatgtt
     8821 ctgcaagaca gatgaatatg gaggtggaga caatgtcttc caaagaacta acaatgtatt
     8881 tagagaaaga attggatcaa ctgaaagaaa aggggttata caatacgatc gacatactag
     8941 aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa gatgatcaat ctagcatcca
     9001 acaattatct tggatttgcc aatcgcgaag aattgaaaaa agcatgtatt gaagcaaccg
     9061 aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa tggttcgctg aaaatccatc
     9121 agcaattaga agaaaaaatc gcagaattca aagggacaga agcagcaatc gcctttcaat
     9181 ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat gacaaaagaa gatgctatct
     9241 tatcggatga attaaaccat gcatctatta ttgacggttg ccgcttatcc ggagcaaaaa
     9301 tcatccgaat taaacatcaa gatatgaaag accttgagaa aaaagcaaag gaagcgatag
     9361 aaagtaagaa atataaaaaa atcatgtata tcacagatgg tgttttctcc atggatggag
     9421 acattgcacg attgccggaa attattccga ttgttgaaaa atatggactg atcacctacg
     9481 tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc aggaaccgtc aagcattttg
     9541 gattaagcga caaaatcgat atgcaaatgg gcacgttatc gaaagctata ggtgttgttg
     9601 ggggttatgt agctggatcg aaaacactaa tcgactggtt gaaagctaga agtcggccat
     9661 tcctattttc tacttcgcta actccaggtg cagctgcagc tgcattggct tcgattactt
     9721 tgatgcaaga acatccggaa cttgtcgaaa aagtttggga aaatgctaat tattttaaag
     9781 aagaattgaa aaaagtaggc tataacatcg gtatgtctga aacacctatt actccagtca
     9841 ttttgggaga cgaaaaagtg actcagactt tctctaagaa gctgatcgaa catgggattt
     9901 atgctaaacc aatcgtttat ccaactgtgc ctcttgggac aggcaggatc agaaatatgc
     9961 cgactgcgga acatacgagg gaaatgcttg atgaagcagt agcagtttat caaaaaatag
    10021 gaaaagaaat ggaaattatt taactaaatt tagttttttc gtctatttaa aattagagaa
    10081 catgttcacg catatattgt taatcatctg aagatagtag taaaaaagat cagggaggat
    10141 catcatgaaa aaagtattgg tgacaggatg tttaggacaa attggttcag agttgatcac
    10201 acggctacgt caagaactag gcagtgaaaa tgtcattgcg acagatatac gcatgcctga
    10261 agtcattgag aaaaacgata tctttgagat cctagatgta atggattacg aaaaaatgcg
    10321 cagcatagca gaacaatatc aagtggacac attgattcat ttggctgctc ttttgtcagc
    10381 tgttgcagaa gcaaaaccac aattagcctg gaatttgaac gtgactgggc tggtcaatgc
    10441 attggaagtc gctagagaat tccagctgaa attttttacg ccaagttcaa tcggcgcgtt
    10501 tggtccaaat actccaaaag atcatacccc acaagatact cttcagcgtc ctacaaccat
    10561 gtatggggta acgaaggtag ctggagaact actatgtgat tattattatg tgaaatatgg
    10621 cgtagatact cgtggtgtcc gtttccctgg attgatttct tatgcaacct tgccaggagg
    10681 cggtacgaca gactacgcag tcgatattta ttattcagca atcaaagaag gaaagtacac
    10741 tagtttcatt gatcaaggta catcaatgga tatgatgtat atgccagatg cgatcgatgc
    10801 cattataaaa ctcatgaatg cagatcctga tcgattggtt catcgaaatg ccttcaatat
    10861 atcggcgatg tcttttgacc cggaagagat caaagcatct attcaaaaaa tcataccaga
    10921 ttttgaaatg gattatgatg tggatcctgt acgtcagtca atagcagaat cttggccaaa
    10981 ttcactagat gtttcttgtg cgcaagagga atggggcttt tcgccgaaat acgacttaga
    11041 tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa ttggtagaga cggtataaaa
    11101 caagaaccac atccaaaaag ttttttatac ttttgggatt ggctatggta tgaaagggga
    11161 caagggatgg aagaatatca agccacgcca gtaaaagaag taaaaataga aaatgaatta
    11221 aaacgtacga tgggattttt tactgcttta tcgactgtta tgggcacagt gatcggtgca
    11281 ggggtatttt ttaaagccgc aagcgtagca gaagttacag gatccgccag tttgcatatg
    11341 ttttcttggt ttctcggtgg tatgatttct gtctgtgcag gactaacagg agctgaactg
    11401 gcagctgcta ttccagaaac aggagggatg attaaatata tcgaacggat ttacggaaat
    11461 acagcagctt ttttattggg ctgggcgcaa gtcgtgattt attttccagc aaatgtagca
    11521 gccttgtcga tcattttcgg tacacagttc gtcaatctat ttggtctttc ccagtcaatg
    11581 attgttcccg tagctgttac agctgctgta tcgatcctgt tgatcaattt tttaggttct
    11641 aaagctggag gggcttttca gtcgattacg cttgtctgca agcttattcc tttgtttgtg
    11701 atcgtcattt tcggtttgtt ccgacaagaa ggagtagact tccaactgtt tccgatccaa
    11761 gcaggagaaa atctttcatt tttctcagcc ttgggagcgg gcttgctagc aacgatgttc
    11821 gcatacgacg gttggataca cgtaggaaat atttcgggag aattgaaaaa gccggcgaaa
    11881 gatttgccaa aagcaatttc tttaggaatc atcggaatca tgatcgttta tttattagta
    11941 aatgcggtgt tcttaagaac cgcatctatc gacggagtcg caggaaacag caatgcggca
    12001 agtgacgttg caaaaatgat ttttggcggc ttcggtggaa gactggtcac agttggtatc
    12061 ttgatttctg tttatggaac catcaatggc tataccctta ctggaatgcg tctcccttat
    12121 gttatggcaa aagaaaacaa cctgcctttt agcaagctct ttgctaaact tcatgataaa
    12181 actaaagttc ctgtagccgc aggtatctta gagctagtca tcgctattgg catgatgatg
    12241 atcggtggtt ttgatacact aacggatatg ctgatctttg tcatttggat tttttatacg
    12301 atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac ctgatttgtt tcgaccatac
    12361 aaagttccaa tgtatccgtt tattccacta gtagcaatca ttggagggac ttttatcgta
    12421 tcaagcacgc ttatcactca gaccttttta gcttcgatgg ggatagtatt gacacttgct
    12481 ggtatcccga tttatctcta tttaaaacgt agatagctaa aacattcgaa ttttattaga
    12541 actgcacttc tctgccaaac aaaatatttt atttaaatga ggagtacgtg gaagaaatgt
    12601 caaaagtact cgttaatttt gatcacgtaa atgtcgtttt gtctacgatg aaatagcaaa
    12661 gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt actttgattt gcgtaactga
    12721 actattcggc gtacgccgcg ataaatattt aataaaacca agcaaccaga aatgaaaatg
    12781 ggaagccaaa tcagatagac agggtagcca agcactttag acaaagcatg tggagtcatt
    12841 ttatccattt caagaagtgc ttgctgccaa gtctgaagat tgaagactag catgaaaccc
    12901 gaaataatca ggagaataaa tcctaaataa tgaattgtag ttctttttct tacataagcg
    12961 atcgtagcat aaaggaacag taaaaaaata ggtacgccaa aaacaaagta catattcagc
    13021 atctcgacac cccctttgtg tttatcttac tttactttga aagaaatgag aaataatagc
    13081 ctcgttactt gcttgaaaca gcaaaggact gctaagataa gagagataca aagaatcaag
    13141 gaggagtcat atggcaagtt atatcgaacc gattcgtttt gcgctgattg tttttccatt
    13201 tttagcctta gcgatctcat ctgttttttt tatttatgaa tatcgaaagt atggaacatt
    13261 tctagtaaca agggcagtta ttttgtattc tttcgttttt tatttattgt gtgcgtattt
    13321 tttggtgatc ttaccacttc cggcaaagga gacggttgcc caaatgacag ggccaaagat
    13381 ggagctgcat ttatttgctt catggcaaca tttcagagac aacacggtat tggtcttgtc
    13441 tgaacctagt acctacctgc cggcaatgaa gcagagtgta tttttggaac cagcttttaa
    13501 tgttttatta ctcataccat ttggcattta tttaagatat tatttccggc tttcttttat
    13561 gaaaacgatg attgctagtt tctgcctttc tctatttttt gaattgacgc agctaagtgg
    13621 actttatttt atctacccaa gaccataccg cttatttgat gtgaatgatt tagtgacaaa
    13681 tacattgggt ggaatcattg gttttgtcgt tacgccgtta tttacattta tgcttcctac
    13741 aagaagacga atggatgaac tttcttatga aaaaggaaag actgtctcat tgactcgacg
    13801 cttggtcgct tatttgatcg actggacatt tttatctctt attaatttgg tagcggcgat
    13861 tttctttaaa attgcgttcg attcaggggc agttacttct catatctggt ggcttttatt
    13921 agaagtaggg gtatacttta tgattgtacc gtttataacc aatggatgga cacttggcaa
    13981 aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa cgaatttctt taaaagcatt
    14041 gatgatccgc tacgggtatc tgtactttct tttttatggt ttaagctggt tggggcagtt
    14101 gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg cagatgagtg cagtattcat
    14161 ttctctcggt gtcttgttac tacaaggtat ttttatttta aatatcttgc tatcattttt
    14221 gagaaaaaaa cggattctgt tttatgaaaa agctagtcac acatatactg ttagcacgat
    14281 tggccaaaca aaggatgatg attctcgaaa ttagctgagc aaaaacgctt tgtctgtgtt
    14341 ataatgccaa agtcgtgata atttaaagag aagaggtgca cgaatgaata attttgatat
    14401 attccgcttc aaaccgttta tcaatgaagc gttaaccgca aagggcttca aacagcccac
    14461 agaggtccag gaacgtttga ttcctttaat tgcaaaagga aaaaatatca ttgggcaatc
    14521 tcaaactggt acaggaaaaa cacatacgtt tttgttgcct ttgtttgaaa aaatcaatgc
    14581 agaaaaagag gaagtacaag ttgttattac agctccaagt agggaactag ctgcacagat
    14641 ctatgaagca gctacacaaa tcgctaaatt ttctacaccg gagatccgtg tctcgaattt
    14701 tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg catcaccaac agccacaggt
    14761 tgtagtaggc acgcctggta gaatattaga catgatgaat gaacaagcgc tgaagattca
    14821 caccgcattt gcatttgttg tcgatgaagc ggatatgaca ttggatatgg gttttttaaa
    14881 tgaagtagac caaattgcaa gtcgattacc agaaaagctg cagtttttag ttttttctgc
    14941 cactattcca gaaaaacttc gtccattctt acgaaaatac atggaaaatc caatcattga
    15001 agaaatcaaa ccaaaagcag ttatttctga agacattgaa aattggctga tttcaacaaa
    15061 aggtaaagat gagaatcaaa tgatttatca actgctgaca attggtcatc cttatttagc
    15121 aatcgtattt gccaatacaa aacagcatgt ggatgaaatt gccgattact tgaaaaacca
    15181 aggcttgaaa gtagcaaaga ttcatggaga catcacccca agagagagaa aacgagtgat
    15241 gcgacaagta cagaatctgg agtatcaata tgttgtagcg actgatttag ctgctagagg
    15301 gatcgatatc gaaggtgtct ctcatgttat taatgcagaa attccagaag atctagactt
    15361 ctttatccat cgtgtcggac gtactggacg taatggctta aaaggaacgg ccattacgtt
    15421 atattcgcca aatgatgagc aagccattga tgcgatagaa aaattgggcg tctcatttga
    15481 accaaaagaa atcaaaaacg gggaaatcgt caaaacatac gatcgtaata gacgtgccaa
    15541 acgagaaaaa tcaaaagatg tattagatcc cacgctgatt ggtcttgtga agaagaaaaa
    15601 gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg gcaattgcag aaaaaaataa
    15661 aaaagaacgc aaaatcgaac gcagacagca aagccgtact gctcgtaaaa ataagaaaaa
    15721 ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt gtcacaatct cttgttttta
    15781 agctagactt tttttcattc cgatacaaag tacttcatct tcaataaaag gttctgtgca
    15841 agtatgatac ccttgttttt cataaaaatc tacagccgtc atttcagcta ctacataact
    15901 agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct tcaagcaact gaccgcctaa
    15961 cccttttcct cggtattctt ttgctacgca aaatcgttcc ggcttgaagc aagtaggtgt
    16021 tttcttttca taacgcaaag tagctactgg tcttttttga tcaagaaata gataataggt
    16081 ttgttgcggg atatcttttc cgtcgaattc tagttctgca ggaatccctt gttctaatac
    16141 aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc cattcatccg ctccaactat
    16201 ttttttcatc atattcctcc ttgtcatgaa tagcagagat gtattgattc ctttattctt
    16261 atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga aaataaaaat tgagatattg
    16321 acagtgatca atagaatgcg tatactaatt aagtacgaaa aggatatgat ttgtttttta
    16381 agactcacag aaaattcttt cttggctgaa aaaagaatag tcactaatca aattgctccc
    16441 tttttctatt ttggtagaaa taccgacgca gctcagcgtt atcgagttta agaggtaatg
    16501 attacacatc attgcaatca aggtggtacc gcgagttttc gtccttggct gtgatggtgt
    16561 ttttttgtat ttaatttata gaaagcaggt aatgacatga aacaattaag tagtgcacaa
    16621 gttcgacaaa tgtttcttga tttcttccaa tctaaaggac atacgattga acctagtgcg
    16681 tctttgatcc ccgtgaacga tccaacctta ttgtggatca actctggtgt tgcaacattg
    16741 aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa gaatcacaaa tgcccaaaaa
    16801 tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag cacggcacca tacgatgttt
    16861 gagatgctgg gtaatttttc gattggtgac tatttcaaaa atgaagccat ccactgggca
    16921 tgggaatttt tgactgatcc aaaatggatg gcattcgatc cagaaaaact ttatgtaaca
    16981 gtttatccaa aagatacaga agcaaaacgc atctggcatg aagaagttgg cctgccacta
    17041 gatcacatta ttgatgtaga agacaacttc tgggatatcg gtgcaggccc atgtggtccg
    17101 gatacggaaa ttttctatga ccgtggagaa gcttataacg atttagcaga agacgatcct
    17161 gaaaactatc caggaggaga aaatgaacgt tatttagaaa tctggaactt agtattttca
    17221 gaattcaacc ataagcctga taacacattt gaaccacttc ctcataaaaa cattgatacg
    17281 gggatgggat tggaacgcat ggtctcaatc gttcaagatg cgtcaacgaa ctttgagaca
    17341 gacttgttta tgcctattat ccacgccgta gaaaagctaa gcaaacaagt aaaatatggt
    17401 gaaaacccag taacagatat ttcttacaaa gtaatcgcag atcatattcg cgccctttca
    17461 tttgctattg gcgacggtgc attgccatcg aatgaaggga gagggtatgt cttacgccgt
    17521 ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta ttgaacaagc attcttatat
    17581 cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt atcctgaagt acttcaacaa
    17641 aaagaattta ttgaaaaagt tatccgtaca gaagaagaac gtttcaatga aacaatcaat
    17701 gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa aaaacgaaaa cgggacgact
    17761 ctagcaggaa aagacatctt caaactatac gacacatatg gcttcccggt agaattaacg
    17821 gaagaggtgg cagaagatga aggactgaaa gttgatcatg ctggttttga aaaagaaatg
    17881 gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg aaaaatcaat gggggtacaa
    17941 tctgctttat tgacagatat caaagtagaa agtaaattca ttggatacac agaacaagaa
    18001 tcagatagtc aattattggt catcgttaaa ggtgagtctc ttttgaatga agtcgcagaa
    18061 ggtgaagctc aaatgatctt tgatcaaaca cctttctatg cagaaatggg tgggcaagta
    18121 gcggatcaag gatggatcat gaatgaagta ggcgaaatcg tagcagttgt tacagatgtt
    18181 caaaaagcgc ctaatggtca atttatgcac accgtagaag taaaagcacc aatgagtgaa
    18241 ggacaaacct atcatctatt gatcgatcaa tcgatgcgca atcggatcat caaaaaccat
    18301 acagcaaccc atctactgca caaggcattg aaagaagtac ttggcgagca tgcaaatcaa
    18361 gctggttctt tagtcgctcc tggacattta cgattcgact ttacccactt tggtcaaatc
    18421 acttcagatg aattagctga aatggaaaga atcgtcaacg aaaaaatctg ggaagctatc
    18481 ccagttgaaa caattgaaac agatatcgat acagccaaaa acatgggtgc aatggctttg
    18541 tttggtgaaa aatacggaca tgacgttcga gtagtaaata ttggcggctg gtcaatcgaa
    18601 ctgtgcggag gcacacatgt agcaaataca gaaaacatcg gaatcttcaa aatcgtttct
    18661 gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg taacaagtaa agaagcgtac
    18721 gaactacttg aagaagaaga aaaacaacta aaacaagtag caacaattgt taaatcacct
    18781 caattgaaag aagttgtttc aaaagcggaa caattgcaac agcaagtaag agagctacaa
    18841 aaggaaaatg aacaattagc tagcaagtta gcgaatcaac aagctggtga tgtattcaaa
    18901 gatccagaaa cgatcaatgg aaaaacaatc attgcagcac aagtaaaagt gaaagatatg
    18961 aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa atatttcaga tattcttgtt
    19021 ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg caatgacgaa agaatcaaat
    19081 gaagctggac taaaagctgg cgacttgatt aaagcaatcg caccgaaagt aggcggtggc
    19141 ggtggtggac gtccagacat ggcacaagct ggcgggaaaa atcctgctgg tattccagat
    19201 gcacttaaag ctgcgaaaga atgggttgcg caacaatagg gcgtttacgt ttgacagaaa
    19261 aacaaaagtt caaacattga acgaaacatg attatggggg cgaagacaaa caagtcttgc
    19321 cctcatactt ttttcaattg gatggcgcat ctaatcaaat ggtcggattc ggatggtctt
    19381 tagttgacga aagtgacaga aatttttacg attagtataa ataaaaaggg gtgaaaaaat
    19441 gaatagtaga aataagacct ttcgactagt cttacgtgcg atcttgttag caatcatcat
    19501 cgtacaagcg atggttcctt ggctggggtt tattccatta ggattcatta gtttaacaat
    19561 tatccatatc acagtaatta ttgctgcagt agtattaggc ccaaaagatg gcatggtgat
    19621 tggcttgttt tggggaatcg ctacaattgt tcgtgcctat gccatgccaa caacgccatt
    19681 tgatacatta gtgttcacga atccgattat ctcggtagtt cccagagtac ttgttggctt
    19741 agtagcaggc ttggttttcc actggattta tcaaaaaaat aaatcaataa cgatcagctc
    19801 agcctttgca ggggtactgg gttcattggt caatacagtc ttagtgttag gattcatggg
    19861 attattttat acaggtgcaa cggcagatgc ctatggtgta gactctgctc ttttatttaa
    19921 aaccttagca ggggttgcag caatcaatgg gataccagaa gcaataggtg cgggaatcat
    19981 aacaccatta ctagcaaaag cgttatttgc agcaacaccg ttgaaaccag aataatgacc
    20041 atcttttaat agtaatagga cgcacgatag atagaaaaag gaagagagga gaggaaaaca
    20101 atcgtcctct ccatcttttt acctgagcaa caagcaaact agaaggggta aaaaacttgg
    20161 ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca gcggacaaaa aaaggagtac
    20221 tagcgggtat cttaggattg gttacgaatc tgatcttgtt tgtctctaag ctaatgatcg
    20281 ggcttttttc aggaagtgtc tccattatgg ctgatgcagt gaacagtcta tcagatactg
    20341 cttcatccat attgactttg atcggattta aaattgctgc aaagccagca gatcaggaac
    20401 atccttatgg acacgaacgc ttcgaatata tcagtgggtt atttgtttcg atcatcatta
    20461 cttatgtagg atttcaattt ttagagtctt ctgctgaaaa aatcatccat ccagaaaatg
    20521 tctctttaac acccatcgtc ttttttgtcc taatcatttc catctttttg aaatttgcac
    20581 aaggcaaaat gtatcaggtt attgcgcaat ccattgattc tgaaacattg agagctacag
    20641 caacagatag ttataatgat gtctttacta cactggctgt attgctttca gcagcaatcg
    20701 aatgggtaac tggatggcgt attgatggat atataggttt tgctttagca ttatatatcc
    20761 tttatagtgg attgacgatg cttcgaaatt ttgtatacga actattagga agtcgcccaa
    20821 cgccagcaga gattgaagaa atggaagagc atttaaatgc ttgcgatgat atacttggct
    20881 atcatgattt attggtgcat aactatggac ccaataaacg ctttgcatca gtccacatcg
    20941 aagtagatga atcaatgaat ttgaatgtgg ctcataagat tattgatctg atagaaaaag
    21001 actttaaaaa gtctttggat gtggaacttg tctgtcactt ggatccaatg cctgttgata
    21061 acgaaaaata ctataatatc ttgcggcagt taaagcaaat tcttcttgaa acaggtacag
    21121 ggcttaatat tcatgatttt agagtaattc atcaaggaaa aacgctgcaa tttgatgtag
    21181 tggtccctga gaaattccag tttgttgata cagaactaga aagaatctta cgtagtcaaa
    21241 tagaagaaaa aatcggagat taccaattgg aaattacgtt tgatcacaat tatcttttat
    21301 attaaatgag aaactataaa aagactcctt aaagttttta atttaaagag tctttttttc
    21361 ttttaattgt ttatggagag cctgacgtgc aagattatct gctcctttat ttttgttttc
    21421 gggcaaccat tgcaggatca ataaagaaaa ttcggataga agttcttgga ttctttgaag
    21481 gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg accgtagaaa ctagtgtttt
    21541 gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg tttttcttta gaaattctaa
    21601 agtttttaaa aagacttcga actctgcttg atggtttgtc cctatagaaa gcgggataga
    21661 tagctgttct tgtttacctt gatagacaat caagattcca cctccactag gaccagggtt
    21721 gccttttgta gaggcatcaa tgtatacttt taacataaaa agagattctc ctttttgttc
    21781 aaactaatgt tatggtaaga ttatatcatt actgaatcgg tgagaggtta gagaatgaaa
    21841 aaaaatattc gatggcagcc tgaactggca catacgatca tctattggtc attgaccttg
    21901 atcattctgt tttttagtct gattctttct ttagaaaaca caggtcctta ttggaaaagc
    21961 aattttgttt taggtatttt tcttgttttt ctatttctag gtttgcggcg ttcttttatt
    22021 ttaaaatcaa actctgtgat tatacgttat gctgcttttt ggaaagaccg agaaatatct
    22081 gtcggacaga ttcgatcttt gacgccaact aatactgggg tgcaaatgat gttgcaagat
    22141 gagaaagccc catctgtttt catgatgcga aaaaaagatc aagtagtcct ttttgatcat
    22201 tttgaaaaaa acggacccaa atcagaatca attccttatc cttatacgaa aaaaagaaaa
    22261 agctaatttt aaaaatcaga acctaaaaaa acgaattttt acaggaaaag aaaagagtga
    22321 gacgtacggt tcatcccgtt tagcgtctca ctctatttgt ttatctattc atgcatacta
    22381 gtatcggtag tctgaacgtt gcctttcttg acatgggcag cttgtgttcc tcgattccct
    22441 tcgatgatct caaattcgac cacttgattt tcttccaaag ttttgaatcc ctcttcttca
    22501 atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat aactgataaa gccgtatcct
    22561 tttttgttgt caaaccattt tacaattcct gtggtcaaag cactcacgac ctttctaaag
    22621 tctgatcata gccttcatta taaagagtat ttataagtac gtcaaataaa ttaaaaacct
    22681 gtaattttat gttttttttc tgaataaaaa aaggtataat tggcatatct taacaaagaa
    22741 gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa atgaagaatg atctgcaaat
    22801 ttcacaagaa acagagctag aaccgattgt tgaagtggct agtaaattag atcttaagga
    22861 agacgatttg gaactgtatg ggaaatataa ggcgaaaatc aatttttctg gaatcaatcg
    22921 atcaaaggat aacgctgagg ggcatttgat cttagttact tccatcaatc caacaccagc
    22981 tggcgaagga aagtcaacga tcactgtagg gttaggagac gcgctcaata aaatcaataa
    23041 aaaatctgtt attgcattgc gagaaccctc acttggtcct gtgatgggaa tcaaaggtgg
    23101 tgctgcaggc ggaggatatg cacaggtatt gcctatggaa gatatcaact tgcattttac
    23161 tggtgacatg catgctatca caacagctaa taatgcatta tctgctcttt tagataatca
    23221 tattcatcaa ggaaatgaat tgaacatcga tgcccgccga gtgatttgga aacgggtagt
    23281 cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc ttgggtgggc caatccaagg
    23341 tgttccaaga gaagatgggt ttgatatcac agttgccagc gaaatcatgg ctatcttgtg
    23401 tcttgcggca gaccttgaag atttaaaggc tcgtcttgca aggatcgttg tcggctatac
    23461 atttgaccgt cagcctgtga ctgctggaga cttgaaagca gaaggcgctc tggcattact
    23521 gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt tacggaacac cagcttttgt
    23581 ccacggggga ccgtttgcca atatcgctca tggctgcaat agtattttag ctaccaagac
    23641 agcattgaga ttagcagatt acgttgtcac agaggcagga tttggtgcag atcttggcgg
    23701 agagaaattt ttagatatca aagtacctaa cttgaaaaaa gcgccggatg ctgtggtgat
    23761 tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg aaaaaagatg aacttaaaaa
    23821 tgagaatctt gatgctttga aagtaggttt tgcaaattta aagcgccata tacgtaatat
    23881 ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa ttcgtaacag atacagacaa
    23941 tgaactcaca ctgttggagc acttgtgtga aaatcagggg attcttgcca agagagcgag
    24001 tgtatgggca gatggagcag aaggcggcgt tgatttagca aaagctgtag tacgattgat
    24061 tgatagaaaa gaagctaatt acaagccatt ataccgccta gaagaaacga tacaagaaaa
    24121 gacagaaaca atcgtcaaga aaatttacgg aggaaatggt gttgttttca gcaaaaaagc
    24181 gcaaaaacaa atagaagagt ttacgaagaa tggatgggac aaactgccga tttgtatggc
    24241 aaaaacacag tattcatttt ctgatgatcc ttcactgcta ggtgcaccgg aagatttcac
    24301 catcacgatc cgcgaaatca tcccaaaatt aggggcagga tttttagtag ccttgactgg
    24361 agatgttatg acgatgcctg gtttgcctaa aaaacctgct gcattgaata tggacgtgac
    24421 aaatgacgga gaagtgttgg gattgtttta agcttgatta taatctacca tcgaaggagc
    24481 tgtattttga ctacagcttc tttttttaga cgatagggaa ttaattttgt ttacactagg
    24541 atcattcttt ttctttattg tattttgagc tatactaaag cataatagga gggtatgatg
    24601 aaaacgacat ttataagaaa aaccggtttt tacggtatgg gtagctcgat tcagctaaga
    24661 atcaacaatg aaaaacgcag tgttctcagt catgaacaaa agataacgct tgatctggaa
    24721 gtgcccttta cgatgcaggt caccttttat tggctaaaaa gccccgttta tacagtgaaa
    24781 gaagcaaagc ctgcttatgt gatcacaatg aattacttga ttcttcaaat ctatccgttt
    24841 ttatttctag caactgggtt gggggccgtt ttcctacaaa atttattttt cagttttttt
    24901 gcagttattg tgttgattgc gttctttttc tatataaaaa accgggcata tttgatcaag
    24961 gaggaaaatt atggcaaact ctgatctttt tttaatcagt ttcaatcgaa tcgaaaaatg
    25021 gatgagagac gagatgggaa atgcacgaaa tatgggattt accgaacttg tcagacgcct
    25081 agctcaaaga aaacaattaa tgatcagaaa atacgaagat gatcttttac agcttgctca
    25141 gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat tttgtcattg cagagccaaa
    25201 tgattggaca gtcaagcgaa tccagctgat cgaacaagaa ctgattcagc ctgaaaccgt
    25261 gctgcctcga tttgcaaaac gtgtaacagg ttttgaacaa gatcttccgt tacttgaact
    25321 gttaaagata gtagcagaaa aaaggtattc acaatttccg ttatacaata aaggaaaatt
    25381 tgtagcattg attactttac gtaatatcgg gttttggcta gcaaaagaaa gccaaaaagg
    25441 tccggttgat ttaacaaata aacgagcgtt ggatttgatc atccaaaacg gaaagtacac
    25501 aaattatcat tttgttccag ctaaaacaca tatttacgag gttgaagcga tgtttcgtga
    25561 gcaagggact ttggaagccg tacttatcac taaagatggg aatcccgatg gtaatctgct
    25621 cgggatcgta cgacctcgtg atatttataa acaaatagaa aaggattgat tttttgttaa
    25681 ttatttattg gatattcagc gctttgctag taggagtgga tcagttggtc aaatggtgga
    25741 tcactgataa tctagcgctt ggagagacga aaaatcttat accaggtatt ttgtcactga
    25801 atcatattcg aaacactgga gccgcatgga gtatgctaga agggaagatg tggttcttta
    25861 ctatagtgac actcattgca gtcgttgtga ttctcacatt gatgattaaa aatcgtgaaa
    25921 aaggtaatcg ctggttcatg attggattga gtttgattct agctggtgcg attggcaatt
    25981 ttatcgaccg tattcgtctt ggctatgtag tagatatgtt ccagacagat tttatgagct
    26041 ttccgatttt taacgtagca gatgtgacac ttgtatgtgg agtgatttgt atgttaattt
    26101 atattatttt agatgaaaag gatcaaagaa aaaaatgaca gaacttaaaa cgacgattca
    26161 ccatgaatct ggtaggatcg ataaagtatt gactaattta ttcaccgatt acagtcgttc
    26221 gcaaattcag ctgtggctga aaaatggagc ggtttctgtc aatggacagc cagtcaaagc
    26281 aaattacaaa gtaaaacaag gagacgagat tactgtcttg atccccgaac cagaaacatt
    26341 agatgtggtc ccagaaaata tcccgttgac gatcgtttac gaagatgaag atgtagcggt
    26401 gataaacaaa cctcaaggaa tggtcgttca tccttcagca ggtcatacgc atggcacaat
    26461 ggtcaatgcg ctgatgtacc atatcaagga tctttctacg atcaacggcg ttattcgtcc
    26521 aggtattgtt caccgaatcg acaaagatac ttccggactg ctaatggttg ccaaaaatga
    26581 ccaggcacac gaatcattag ctcagcaatt aaaagataag acctctttgc gcaaatatgt
    26641 tgctttagtc catggagaga ttcctcacga aaaaggaacc atcaatgcgc caatcggccg
    26701 ttcgaaggaa aaccggaaaa tgcaggcgat aagagaagat gggaagtcgg ctgtcacaca
    26761 ttttactgta ttggaacgtt tcgaaggatt tactctggtt gaattaaaac tagaaaccgg
    26821 aagaacacac cagatccgtg tgcatatgaa gtatatcggt tatccgttag ctggagatcc
    26881 agtatacgga ccgaagaaaa ctttgaaagg caatggtcag tttctccatg cgcaaatgtt
    26941 aggttttacg cacccgacta caaacgaaaa gatgatcttt gaagcacctc ttccagaagt
    27001 ttttgaaaaa actttagaaa aattaagaaa aaacattgca ttttaattta gataacggta
    27061 tagtaattaa caacgaaagc ataaaactta atagaattcc tttaagttta gtcccgtgag
    27121 gctaagaagg gtgggagcaa ggatacgtat ggtgcgccat aagtagattg ctcattgaca
    27181 tagtatagct atcagtgtat cagtatacgc tcagtctaaa cattcctcct gtcctcttag
    27241 ggcaggagtt tttttatgca aaaaagtagg gggaatgaaa atgcaagcaa aagaagtagt
    27301 agatcaggta accatgaagc gagcattgac tcgaattact tatgagatta tcgagagaaa
    27361 ccatagtatc caagatatcg tattggtagg tatcaaaaca cgaggaatct acattgcatc
    27421 aagaattgcc gaacgcctga agcagctaga agatatcgat attcctgtag gagaactgga
    27481 tatcacttta taccgagatg acaaaaaaga aaatccagaa gaaccggaac ttcattcttc
    27541 tgacatccca gtttccttag aaggaaaaga agttattttg attgatgatg ttttgtatac
    27601 aggacgaacg attcgagcag caatggacgc agtaatggat tttggcagac caagaaaaat
    27661 ttcattagca gtcctggtcg atcgtgggca tcgagaattg ccaatccgtg cagattatgt
    27721 tgggaagaac attccaacat ctcgggcgga agagatttta gtagagatgc aagagcttga
    27781 tggacaagat cgcatcatga ttttgaaaga ggaagattag aggagagaga aacatgtcgg
    27841 agaaaaaagg atttcataat gatgacgtgg tgttagatat ccacgatcgc ccaaccccag
    27901 gacattgggt aggattaagt ttacaacact tgttcacgat gttcggagca accgtgttag
    27961 tccccatctt agtaggtatc gatccaggta ttgcgctagt cagttcagga ctaggtacat
    28021 tggtgtactt atttactact aaaggaaaga tacctgcgta cttaggaagc agcttcgctt
    28081 tcattgcagc gatgcagctt ttgatgaaaa cagatggtta tccagcgatt gcacaaggag
    28141 cagtcacaac tggattagtc tatttgattg ttgcgctgat catcaaaaag atcggttcag
    28201 catggctgga taaaatcttg cctccaatcg tcgtcggtcc agtcatcatg gttatcgggt
    28261 tgggacttgc ggcaaatgca gcaacaaatg ctatgtataa tcagggcacg tatgatttca
    28321 agtatattgc tgtagcgctg atcacattag gattaacaat tttcttcaac atgtggctaa
    28381 gaggttttct cggattgatt ccgattttac taggtattat tagcggatac atcgtagcct
    28441 tgctgtttgg aatcgtggat actaagccaa tcgtagacgc tgcctggttt gctgttccta
    28501 actttgagat tccctttgta caatatgaac caaagctata tcttggagca atcacaacaa
    28561 tggcacccat cgcttttgtg acgatgacag agcatatcgg ccatcttatg gtattgaata
    28621 aactaactaa acgtaatttc tttgaagaac cgggtcttca taagacgttg acaggtgatg
    28681 gattagcaca aatcgtggca gcctttgtcg gaggtcctcc tgtaacaagt tacggtgaaa
    28741 acattggtgt tctggctatc acacgtgtcc atagcgtgtt tgtgatcggt ggagctgcag
    28801 tatttgcagt cattttagga ttcgtaggaa aattaagcgc tttgatttta agtatccctg
    28861 gaccagtcat ctcaggaatc agttttatct tatttggagt aattgccgca agtggaatga
    28921 agattttaat cgagaacaaa atcgattttg ataaaaagaa aaatctgttg attgcttcag
    28981 ttatcttagt agtaggaatt ggcggactcg tctttgaagt aggcacattt acattgtcgg
    29041 caatggcatt agcaactgta cttggcatca ttttaaatct tatcttacca gaaacttcac
    29101 gaagcgaaga aaactaagga ggaaacatta tgatcatcaa atcagaacgt atcagcttaa
    29161 agcatttatt gaccgttgag gcacttactg atcaagaagt tatgggcttg atccgcagag
    29221 ggcaggaatt caagcaaggt gctaattgga ctccgcaaaa gcgccagtac tttgctacaa
    29281 acctcttttt cgaaaatagt actcgcacac ataaaagctt cgatgtagcg gagaaaaaac
    29341 tagggctaga agtgattgaa tttgaagcaa gcacaagctc tgtccaaaaa ggggaaacac
    29401 tatacgatac agtactgacg atgtcagcat taggcgtaga cgtagcggtt atccgtcatg
    29461 gtgatgagaa ctattatgac gaactgatac aaagtaaaac aatccagtgt tcgattatca
    29521 atggaggaga cggaagcgga cagcatccaa cccaatgctt gcttgatttg atgaccattt
    29581 atgaagaatt tggtcatttt gaaggtctga aagttgccat cgtaggtgat atcactcact
    29641 ctcgcgtagc taaatccaat atgcagatgt taaaacgtct aggcgcacaa gtattcttct
    29701 caggtcctag agaatggtac gacgatgaat tcgaagtgta cggacattat atgccattgg
    29761 atgaactact agatcaagtt gatgtcatga tgatgcttcg ggtgcaacac gaacgccatg
    29821 atggaaaaga aagtttctca aaagaaggct accatcagga acatgggtta acagttgagc
    29881 gtgcgaagaa gttgcaaaaa catgcaatca ttatgcaccc agcaccagtt aaccgtgatg
    29941 tagaattagc tgattcatta gtggaaggat tacagtcgcg gatcgttcag caaatgagca
    30001 atggagtatt cgtcagaatg gcaatcttag aagcagtgct aaatggaaaa gcgtaattat
    30061 aggaggatga aaaaatgaaa acactcatta aaaacggtca gatcaataca cgaaaaaata
    30121 tgacgacacc agcagaaatt tggatagaag atgggcgaat caaagcaatc ggtacaggat
    30181 tctcagaagc cgaatttgat gaagtgtttg atgcaaaggg acaattgatc acacctggtt
    30241 tagtggacgt ccatgttcat ttgagagagc caggatttac atacaaggag acgatcgaag
    30301 ctgggactcg ctcagctgct cgtggcggtt tcactactgt atgcgcgatg ccgaatttaa
    30361 atcctgtacc tgatacggca gaaaagctga gacaagtata tgacatcatt agaaaagatg
    30421 cagttgtgaa ggtattgcag tatgcgccaa tcacagaaaa tctgcgaagt gagaaattag
    30481 ttgatcaaga agcactgatt gaagaaggtg cttttgcttt cacaaatgac ggtgtaggtg
    30541 tccaaacagc tgggacgatg tatctggcta tgaaagaagc tgcaaaaaac aataaagccc
    30601 tagtagccca tacagaagat gaatctttat tatttggcgg cgttatgcac gctggcaaga
    30661 aggcagaaga acttggtctt cccgggatct taagcgtaac cgagtcttct caaattgcta
    30721 gagatctgtt gctagctgaa gcaactggtg ttcattacca tgtttgccat gtttctacta
    30781 aagaaagtgt ccgtgtaatc agagacgcaa aaaaagcagg aatccacgta acagcagaag
    30841 tttcacccca tcatcttatt ttgatcgacg aagatattcc cgaagatttc ggcttttgga
    30901 aaatgaaccc gccgctaaga ggacgggagg acagagaagc tttgatcgaa ggcttgcttg
    30961 acggtacgat cgattgcatc gctacagacc atgcccccca tggattagaa gaaaaaagcc
    31021 aaagtttcat gaaatcacca tttggtatcg taggaagcga aacagcgttt caactgatct
    31081 atactcattt tgtagaaact ggtcgattca cgttggaaca agtgatcaat tggttggcag
    31141 ttaaaccggc tgagattttt ggtttgaatg caggaacgct aacggttggt gcccccgctg
    31201 atgtagcagt ttttgatatt acacaaactt gcacgatcga taaagaagac tttttatcaa
    31261 aaggggagaa tacacctttt atcggctgga aagtaaaagg cgaaacacaa atgacttttg
    31321 tcaacggaaa acttgtgtgg cagaagggag aataaaagta tggaacgttt gcttatatta
    31381 gaagatggaa cagttttcga aggaaaggct tttggtgcag atgctagtgt cttcggagaa
    31441 atcgttttta caacaagtat gactggttat caagaaacta tcactgatca aagttttaac
    31501 ggtcaaatca ttacatttac ctatcctatg gtgggtaatt atggggtaaa tagagatgat
    31561 tatgaatcga tttctccaac atgcaaagga gtagtagtaa aagaacatgc acgtgtagct
    31621 tcaaactggc gaaaccaaat gacgctagat tcatttttga aacaaaaagg aattcctgga
    31681 atatcaggta tcgatacgcg cgcccttact cgcaaaatcc gtgaagtcgg aacgatgaaa
    31741 gctagtatag ttgatgctgg agattccttc gaacatgcat ttgatcagct gaaagcaacc
    31801 gttctagcaa ccaatcaagt gcagcaagtc tcaactaaca aaccttatcc aagtccagga
    31861 acaggtcgga aagtcattgt cattgacttc ggcttgaaac atagtatttt acgagaattg
    31921 agtaaaagaa actgtaacct cacagtactt ccctataaca cagatgccga gacgatttta
    31981 gcgctatcac cagacggagt catgctgacg aatggacctg gtgatccaaa agatgtgcca
    32041 gaagcaatcg aaatgatcaa aaaaatccaa ggaaaagtac cgatttttgg tatttgtctc
    32101 ggtcaccagt tatttgcttt agccaacggt gcagatacct ataaaatgaa attcggacat
    32161 cgaggattaa atcacccggt acgagaagta gcgaccggaa gaattgattt tacttctcaa
    32221 aatcatggct atgcagtcga tgaagccacg attgatccag aaaaactcat cgtaactcat
    32281 gtagaagtaa atgacggaac agtagaagga gtcaaacaca gagattatcc cgctttcagt
    32341 gtgcagtttc atccagatgc tgcccctggt ccgcatgatg cactacattt atttgatgaa
    32401 tttatggaaa tgatggacgc aggaaaggag cagtaaacag atgccgaaac gtacagatat
    32461 caagaaaatt atggtgattg gatctggtcc gatcatcatt ggtcaagccg cagaatttga
    32521 ctacgcagga acacaagcct gccttgcctt aaaagaagaa ggatatgaag tggttttagt
    32581 aaattctaat cctgcgacga tcatgacgga taaagaagtg gcagatcaag tatatatcga
    32641 accgatcaca cttgaatttg tatcaaggat tcttcgaaaa gagcgtccag atgctctcct
    32701 tccaacttta ggcggacaga caggcctcaa tatggcaatg gagctggcag catcaggtat
    32761 tttagaagaa ttgaacatag aacttctagg aacaaagctt tctgcgatcg atcaagcaga
    32821 agatcgtgat ctattcaaac agttgatgaa agaattgaat cagccaatcc ccgagtctga
    32881 aatcgtcaat acagtagaag aagctgtaga ttttgcaaat aaaatcggct atcctgtcat
    32941 cgttcgtcca gcctttactt taggcggaac tggtggaggt atgtgtgaaa atgaagaaga
    33001 attacgtatc gtatcagaaa atggattaag tctttccccc gctacccaat gtttgattga
    33061 acgaagtatt gcaggtttta aagaaattga atatgaagtg atgagagatt cggctgataa
    33121 tgcaatcgtt gtctgcaata tggaaaattt tgacccggtg ggtatccata caggtgattc
    33181 tatcgtattt gcccctagtc aaacattgtc tgaccatgaa tatcaaatgc ttcgtgatgc
    33241 ttctctttcg attattcgag cacttaagat cgagggaggc tgtaatgtcc aactcgctct
    33301 tgatccacat agttttaatt attatgtgat tgaagtcaat ccgcgtgttt ctcgttcatc
    33361 tgcgttagca agtaaggcga ctggctatcc gatcgctaag ctagctgcta aaatagcgct
    33421 tggattaaca ttagacgaaa tgaaaaatcc tgttacagga acgacttttg cagaatttga
    33481 acctgcatta gattatgttg tagctaaaat tcctcgctgg ccgtttgata agtttgaaaa
    33541 cggagaacgg cttttaggca cgcagatgaa agcaactggt gaagtgatgg ctatcggtcg
    33601 gaatatcgaa gagtctttat tgaaagctgt gcgttcttta gaaatcggta cacaccatat
    33661 cgaacttcct gagttggcag acatcacgga cgatgaccta atccaaaaag tggttcgagc
    33721 acaagatgat cgcctgtttt atgtagctga agctattaga agagggtaca ccatagaaga
    33781 attagcggat ttgacaaaaa tcgatttatt ctttttagat aaactgttgc atatcgtaga
    33841 aatcgaacag cagttgaaac agcagccgtt ggatgacaag atattgaaaa cagcgaaaca
    33901 aaacggtttt acagacaaaa aagtggcgga gctgtgggaa ataaacgaag aagccgttcg
    33961 ccagatgaga aaagaaaatg atttgattcc tgtatataag atggttgaca cttgtgcagc
    34021 ggaattcgaa tcaaaaacac cttattttta tagttcctat gaatatgaaa atgaaagttc
    34081 acgatcagaa aagccctcaa ttctagtact gggatctggt cctatccgta tcggacaagg
    34141 ggttgaattt gactatgcga ctgtgcattc tgtcaaagca atccagcagg caggatacga
    34201 agcaatcatc atgaacagca atcctgaaac agtttcgact gacttttcta tttcggataa
    34261 attatatttt gagccattga ccttcgaaga tgtgatgaat gtgatcgagc tagaacagcc
    34321 gataggagta atcgttcaat ttggtggtca gacagcaatc aacttagccg aacctttgac
    34381 tgctgctggt gtaaaaatct tagggacaac gatcgaagac ttggatcgtg cggaaaatcg
    34441 tgatttattc gagcaagcgc tgaaagagtt ggatattcct caacctttag gggatactgc
    34501 tacaagtaaa gaagaagccg tggttatcgc ttcgagaatt ggttatccag ttcttgttcg
    34561 tccaagctat gtattaggcg gacgtgcgat ggaaatcgtt gaaaaccagc gagatttaga
    34621 agattacatg gaacatgctg tgaaagcatc tccagaacat ccagtactag tggaccgtta
    34681 cttgcttggt ggtgaatgcg aagtggatgc gatttgtgac ggtgaaacag tactcatccc
    34741 agggatcatg gagcatattg agcgtgctgg tgtccattca ggggattcaa tggctgtgta
    34801 tccacctcaa acattatctg aatcgatcaa gaaaacgatc gaagattaca cgatccgctt
    34861 agcattaggt ttgaattgtg tcgggatgat gaatatccag tttgtcatcc atgaaaatga
    34921 ggtttatgtg atcgaagtca atcctcgagc aagtcggact gttccattct taagtaaggt
    34981 gacaggaatt ccaatggcac aaattgcgac gaaagctatt ttgggagaga aactaaagac
    35041 acttggttat gaaaacggac tttatccaga aacaaaagaa gttcatgtta aagcacctgt
    35101 cttttctttt acgaaattgc agaaagttga tacttactta gggcctgaaa tgaaatcgac
    35161 tggagaagta atgggatctg acagacaatt agacaaagct ttgtacaagg cattcgaagc
    35221 ttctggatta cacttgcctg aatatggagc cgtattattt acgattgcgg acgaaacaaa
    35281 agaagaagca ctagaattag ctaaacgttt tacagaaatt ggctatagtc tgataggaac
    35341 gaaacataca gctgcttatt tcgaaaaaaa tggtctggtc gttgaaacag tagcgaaaat
    35401 ttctgaggaa gcagcagaaa aaaatgtagt cgacttaata agagatggta aaacacaagt
    35461 ggttgtcaat acgattgata aagatcgggg aaatgcttct aaagacggtt tcatcatccg
    35521 aagagaagca gttgaacatg gtattccatt gtttacttct ttagacacag cagatgcgat
    35581 cgttcgagtg atggaatccc gtgctttttc aactaaccct atttaacggc gagcacatgt
    35641 taagacaaaa agacgaaaaa ccgttgcagg aggggacaga tgaaacaaga aatgatggaa
    35701 atcgttcagc aaaaacagct ggcaccaaag atttttgaaa tgacattgaa aggtgatcta
    35761 gtaaaagaaa tgcagcaacc aggacagttc ttgcatattc tcgtgccgcg tgcagacttg
    35821 ctattacgcc gaccgattag cttgaatcat atcgaccacg aaacaaatac ttgccgtatc
    35881 atttatcggg tggaaggtga ggggactaga gtcttctcta ctctttctgc cggagaccat
    35941 ttagatgtca tgggtccttt aggcaatggt tttgatttgt ccaatcttca tacaggagac
    36001 gaagtattta taatcggtgg aggaatcggt gtccctcctc tatatgagtt atcaaaacag
    36061 ttagtcgaaa aaggaattaa gccaacgcat tttttaggtt ttgccacaca tgaagtcgtt
    36121 tattatgaaa aagaatttaa tcgtctagga gaaacaagaa tcgctactga cgatggaaca
    36181 tacggtatgc acggaaatgt cggtaattta ttgttaggaa cgaagacgga acctacagct
    36241 gtttttgcat gtggttcaaa cgggttattg aagacagtgg aacagctgtt ttcttcgcat
    36301 caaaatgtcc agttatcatt agaatcccgc atggcatgtg ggataggggc ttgctatgct
    36361 tgcgtttgtc atacgccgga agatgaaaca ggaacaaaaa gtgtgaaagt ttgtgacgaa
    36421 ggaccgattt ttaaaatagg agaggtaatt atatgaatga gaatccttta gcggtcaaac
    36481 ttcctggcct agaattaaaa aatcctatca tgccggcaag cggctgtttc ggttttggca
    36541 aagaatacgg aaaatactat gatttgaatc agcttggatc gatcatggtc aaggcaacga
    36601 ctccaaatgc ccgctttggc aatcccacgc caagagtggc ggaaacacca tctggcatgt
    36661 tgaatgcaat tggtttgcaa aatcctggtc tagacgttgt tatgcataaa cttcttccag
    36721 aattagagga gtacaatgag ttacccatca tcgcaaatgt tgctggtgct tgtgaagaag
    36781 actatgtaga agtttgtagc aaaattggcg aagcaccaaa tgtgaaagca atcgagctga
    36841 acatttcttg cccaaatgtc aaacatggcg gaattgcttt tggaacagat tctgatgttg
    36901 cttttcaatt aacacaagca gtaaagaaag tttcttctgt tcctgtctat gtcaaattgt
    36961 ccccaaatgt aacagacatc gttccaatcg ctcaggcaat cgaagctgga ggggcagatg
    37021 ggtttacgat gatcaatact cttcttggta tgcggattga ccttaagaca agaaaaccga
    37081 ttttagccaa tcaaacaggt ggactctccg gaccagcaat caagcctgtc gctattcgtt
    37141 tgattcacca agtagcaagt atatccagcc ttccaatcat tgggatgggc ggcgtgcaga
    37201 cagtggatga tgttcttgag atgtttatgg ctggagcaag tgctgtagct atagggaccg
    37261 cgaatttcac cgatccgtat atctgtccta aactaattaa agagctccct gtgcgaatga
    37321 gcgaactagg catcgaatcg ctggaaagat taatcaaaga agtgagagag gaacgtgaac
    37381 gatgaatcaa ctagaccaac gaccaattat tgcattagat ttttcaacta ggcaagaggt
    37441 agaagacttt cttcgatttt tcccgaaaga agaaaaattg tttgtgaaaa tcggaatgga
    37501 attgttctac caagaaggac ctgaaatcgt acgttatcta aaagaggctg gtcataatgt
    37561 atttttagat ctgaaacttc acgatattcc gaatacggtc gaaaaagcaa tgcggggatt
    37621 ggcaaagctt ggtgttgatc tcacctgtgt tcatgccgct ggcggtattc gtatgatgga
    37681 ggcggcaatg cgaggattag aagaggggac tccagaaggt ggaaaacgtc cgctacttct
    37741 agctatcact cagcttactt ctacaagtga agaagaaatg catgccgatc aactaatcga
    37801 agtttcttta gaaaaaagtg tgatccatta tgctagctgt gccaaaaaag caggattaga
    37861 tggtgtggtt tcttctgctt gggaagtaga agcgatcaaa gaaatggcag gagacgaatt
    37921 tgtttgctta actccgggta taagaccaga aggaacagtc gcaggagatc agacaagagt
    37981 agtcacacct tctcaggcaa aaaagatcgg ctcaactttt atcgttatag gtcgaccaat
    38041 cactcagtca actaatcctt ataaagcata tcaaacgatt caaacagaat ggagctaacc
    38101 taaaatgaat gttgaacaaa gtatcgcaaa agacttatta gaaattgaag cagtattttt
    38161 aaatccaagt aatcctttta catgggcaag tggaatcaaa agcccaattt actgtgataa
    38221 ccgaatcacg atgagctatc caaaagtccg aaaagagatc gcaaaaggat tagcaagcaa
    38281 aatcaaagaa gcttaccctg aagtacaagt gattgctgga actgcaacag caggaatccc
    38341 tcatgccgca tgggtagcgg aaatactgga tttgccaatg gtctatattc gaagtaaggc
    38401 gaaagatcat gggaaaggta accaaatcga aggacgtata tttgaaggtc agaagatggt
    38461 tgtgatcgag gatttgattt ctacaggcgg aagtgtcttg gaagcagctg aagcagcaaa
    38521 aagagaaggt gcggatatcc ttggtgttgc ggctatcttt acgtatgagt tgccaaaagg
    38581 aaaagcaaat ttcgaaaaag ccgaaatccc tcttatgacg ttgaccaatt attctgtatt
    38641 gattgaagca gctttagaag atcgttatat cgatgagcaa gaattaactc tgctaaaaga
    38701 atggaaaaaa gatcctgaaa attggcagat tgaagcgcgc ttgatgaaga tagagaaatc
    38761 cgatttgtgt ctccagaatc atcaattgga atagcaaaaa tccaaaaact gattgcgaga
    38821 agcatctttt catcaggtat gaaaagaaac ttctatcgtg atcagttttt gattttttta
    38881 aaattcattt ttctaagact ggttagcaga aagaagagaa taatctcttc tcttttttct
    38941 attatatata gattttctct attttttggt aagggagctg tttcaaaaat ggtaaagagc
    39001 ggaaatgttg cgaatatcag ttatttaatg atatgatatt cggaaaaaga aatctcaaag
    39061 ggaggtaact tttatggggt tgaatgaatt tattttagga ttgaacaatt tagagacgat
    39121 cacaagggca cctggatttt ttaaatatac agagcataca gttgcagcac attcgtatcg
    39181 tgtagcatct attgcgcaag ttctaggaga cattgaagaa gcgaatggac aaccgatcga
    39241 ttggaaactg ctctatgaaa aatcattgaa tcatgactac acagagcgtt ttattggtga
    39301 tatcaaaacg ccggtgaaat acgcaagcaa agagttaaga gggatgctgc aaacagttga
    39361 agaaaagatg acggacgaat tcatcaacca agagattcca gctgagttcc aagaagttta
    39421 tcgccgtcgg ttgttcgaag gaaaagatga aaccgtagaa ggagaactat tagccatttc
    39481 agataaggtt gaccttcttt atgaatcttt tgatgaaatc atcaaaaata acccggaaaa
    39541 agtttataag gagatgttca ttgaggcagt gactacgatt aaaagctttg aacatcggcc
    39601 ttctgttcag tatttcttta aagagatatt tccggaacta ttgaaccagg aattttatgg
    39661 aaaagaggcg tttttagaaa ccgtctcaac gattgtaaag aaagaagaaa aataaaaatg
    39721 atatgagatg cagggggaag tatagttgaa aaagaatatg gatgtcgaat ggggctattc
    39781 tggagacaaa ggtccggaaa attgggcgag tttatgtgat tggtttgcga gaggtgcgga
    39841 atttccattg cagtcgccta ttcatttgac caagggggaa gaaaccaaaa tagaaggaga
    39901 cacgctttcc tttcattatc aaaagcaacg gtttactgac aaggaattca aaaacacgat
    39961 tcatttagta ccttttgacc aactcagtta tgtagagttc aagaaagaac gctattattt
    40021 gacagatatt catttccact tgccttctga acatatcatc aacggaaaac aggaagatat
    40081 tgaatttcat tttgttcata tgaacagcaa aaaagagaat ctagttgtgg gtgtcatgta
    40141 ccagctaatg gtacaggaag gttggctgta taatcaagaa aatggggaaa gttggaactt
    40201 agaaaaacat gagcattggg taaatccgga tgtgttcttt ccggagcaaa aaagccattt
    40261 ccattatatt ggctcattga caactccgcc taccagcggg ccaatccaat ggtttgtaat
    40321 ggatcatgta ggaaaactga accaagagtt tcttctcccc ttcgatggtc agtatgctcg
    40381 accgaacaac cgaccaatcc agcccttgaa tggaagagaa atttattatt tcgaggagtt
    40441 tgagcaatga atattcagca gatgaaatac gtggtcgctg tagcaaacaa tggtagtttc
    40501 agagaagcgg caaagaaaat gtttgtctcc cagccaagtc tttcagctgg tatcaaagaa
    40561 ttagaaaccg agttagggat cacgctgttt actcgaacga atcgaggggc atatttaaca
    40621 gaagaagggc aagacttctt aaatcgtgcc gagaaaatat tggtccagct tgatacatta
    40681 gaaactcact atctaacgag tgaaaagaga gacaattttt cgatcgctgc ccagcattat
    40741 gatttcttag gtcctttgac cgcacagcta ctcgaggaat tcaaagacga gatcaaacaa
    40801 tttcggatca tggagacgac aacagcgaga gtgatcgaag aagtcaaaga gcagcacagt
    40861 gagatcggca ttatttatat gaatgagcat aatcaagctg gtatcagaag atatttacaa
    40921 caaggagagc taagtggccg cagcattgga aaattcaaaa cgcatatttt tttaaggcaa
    40981 ggacatccat tagctgaaca cgcagagatc accaaagaag aattgttatc ttatcctcaa
    41041 gttcgtttta cacaggatgg aaataatttc ccgtattttt atgaagacct tatcgagatc
    41101 cctgatcaag aaacagtgat ttatacgagt gatcgaggta ctttgatgaa tatcgttctt
    41161 gaaactgatg cttacgcttc aggatctggt attgtgattg gagggattcg cgagcacttg
    41221 cggttaatcc ctttggcaga aggcgaacta aatgaatttt atatcattta ttcggcgaaa
    41281 cgtccgctca gtgaaatcgc tcaaagattt atcaaagagc ttactcggtt actggaccag
    41341 cagagctaag aaaagcagat gagaacgctg tttattgaac accgttgatc aaaaaagagg
    41401 ggttgtgaca gaattttgtc acaactcctc tttttgtagc aaacaattta taactaattg
    41461 ctgttgtggg aaagtacgca aatatgattc tagaaacact cattgattac ctgttttgtt
    41521 tcatagctgt aattttttct tcttctggtg tcacgataat cgtcttttgg ttttcataaa
    41581 taacataacc aggcttggcg ccatttggtt ttcggatatg ctttacctgt acaagatcaa
    41641 ccggcacttg ggcagagtat cggtattttg aaaaataggc agctaactcg gcagcttctg
    41701 taattgtttc atctgagggc ttgtcacttt taatgatgac gtgcgaaccg ggtatatttt
    41761 ttgcatgaag ccaatagtcg gtttttttag ctgtcttcat tgtcagctgg tcattttgaa
    41821 gattgttttt accaacaaga atgagcgttc cgtcagaaga aaagtattga tcaggctgag
    41881 atggtttctt acgtttttgt ttttttaagt tcttcttttt caggtatcct tctgaagtca
    41941 attcttcttt aattgcttcg atatccattg ggccggcaat ttccagttga gataagacag
    42001 attcaagata ttggatttca tctttggctt cttggatttg ttcgccaatc agcttaacag
    42061 cgttcttcag cttttggtat cgatggaaat atttctgtgc attttgattt ggtgtcaatg
    42121 ccggatctag tgctattttg attggccgat cttcttcata ataattaggt aaaacaactt
    42181 cttttgcgcc gcgtggtact tgtgtcagaa aagtagtaag tagttcgcca tttctacggt
    42241 aattctcagc gttttcggat tctttgagtg tttgttcacg ttttttcaat ttatttttat
    42301 ttcgttttag ttcattttcg atttttcgaa tcagttctcc gccttgttgt tttgcccgat
    42361 ctttctctgc tttttcgtga taatagctat ctaataattg actgagtgtt ggataagctg
    42421 ttacagcaga tgcctgttcg gacaatacct gataagcaat cggtgtgaaa aaatctttat
    42481 tttccgtttc gtaaaaagta ggaataggct gatggctgat ggctgagaaa aattcgtccc
    42541 atacaaccat cttttcatta ggacgttcca gtaatctttt ggtcagctct tgagcgatat
    42601 cgaaaccgat tccctgaaac tgacgttgga ttgcttttgg atctagttca gtttgggaaa
    42661 gccgctgaaa gattttttct ttctcggagc taaatgggtt caagacctct tgttttggcg
    42721 gagcaacata ttctacacca ggaagcaaag aacgataagt gttttgagag cttccaatgt
    42781 gtttgatcgc atccagtatt tttccagttt ctcgattaac taagacgatc gtactatgac
    42841 gtcccattaa ttcaacgatc aatatgatat tttgtagatc acccaactcg tttcgtttag
    42901 caaagtggaa atggatcact cgatcatttt cgatttgttc gatgtcttct aaaatagcgc
    42961 catccaaata ttttcgaagc atcatcacga agttaggcgg attatcagga ttctgataat
    43021 cgatttgggt aatctgtaca cgagcataac ttgggtgcgc agataataat aatcgatggt
    43081 ttttgccgcg tgaacgaatc accagcacga tttcattttc ataaggctga tggattttcg
    43141 agatccggcc agatagtaac gtttcacgca attcgttgac catcgcgtgg gtaaatacac
    43201 catcaaagga catggttcat tcctcatttc ttttcataag tatattcatt ataacgaaaa
    43261 aaagaagaaa aaccaatttg gccgaatagt aaagcaaaag tctagaaaaa tcttggacta
    43321 attacataaa aaacagtgtt tctatcgttg aaatccaaat ttttaggta
//
LOCUS       DARBFO010000013        41117 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000013 DARBFO010000000
VERSION     DARBFO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.013"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(403..1185)
                     /locus_tag="SCK51_000862"
     CDS             complement(403..1185)
                     /locus_tag="SCK51_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251506.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1250..2086)
                     /locus_tag="SCK51_000863"
     CDS             complement(1250..2086)
                     /locus_tag="SCK51_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1251507.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2104..3513)
                     /locus_tag="SCK51_000864"
     CDS             complement(2104..3513)
                     /locus_tag="SCK51_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1251508.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3527..5350)
                     /locus_tag="SCK51_000865"
     CDS             complement(3527..5350)
                     /locus_tag="SCK51_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1251509.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5630..5857)
                     /locus_tag="SCK51_000866"
     CDS             complement(5630..5857)
                     /locus_tag="SCK51_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251510.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
     gene            complement(6193..6519)
                     /locus_tag="SCK51_000867"
     CDS             complement(6193..6519)
                     /locus_tag="SCK51_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251511.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6565..6927)
                     /locus_tag="SCK51_000868"
     CDS             complement(6565..6927)
                     /locus_tag="SCK51_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEG1251512.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6964..7179)
                     /locus_tag="SCK51_000869"
     CDS             complement(6964..7179)
                     /locus_tag="SCK51_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1251513.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7306..7641
                     /locus_tag="SCK51_000870"
     CDS             7306..7641
                     /locus_tag="SCK51_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1251514.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7717..9714)
                     /locus_tag="SCK51_000871"
     CDS             complement(7717..9714)
                     /locus_tag="SCK51_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251515.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9812..10087)
                     /locus_tag="SCK51_000872"
     CDS             complement(9812..10087)
                     /locus_tag="SCK51_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1251516.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10319..11053
                     /locus_tag="SCK51_000873"
     CDS             10319..11053
                     /locus_tag="SCK51_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1251517.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11092..12234
                     /locus_tag="SCK51_000874"
     CDS             11092..12234
                     /locus_tag="SCK51_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1251518.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12329..13720)
                     /gene="sufB"
                     /locus_tag="SCK51_000875"
     CDS             complement(12329..13720)
                     /gene="sufB"
                     /locus_tag="SCK51_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEG1251519.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13725..14195)
                     /locus_tag="SCK51_000876"
     CDS             complement(13725..14195)
                     /locus_tag="SCK51_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEG1251520.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14182..15417)
                     /locus_tag="SCK51_000877"
     CDS             complement(14182..15417)
                     /locus_tag="SCK51_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG1251521.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15414..16700)
                     /gene="sufD"
                     /locus_tag="SCK51_000878"
     CDS             complement(15414..16700)
                     /gene="sufD"
                     /locus_tag="SCK51_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEG1251522.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16713..17486)
                     /gene="sufC"
                     /locus_tag="SCK51_000879"
     CDS             complement(16713..17486)
                     /gene="sufC"
                     /locus_tag="SCK51_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEG1251523.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17683..18531)
                     /locus_tag="SCK51_000880"
     CDS             complement(17683..18531)
                     /locus_tag="SCK51_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEG1251524.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18555..19241)
                     /locus_tag="SCK51_000881"
     CDS             complement(18555..19241)
                     /locus_tag="SCK51_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1251525.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19234..20268)
                     /locus_tag="SCK51_000882"
     CDS             complement(19234..20268)
                     /locus_tag="SCK51_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1251526.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20331..20587)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20663..21001)
                     /locus_tag="SCK51_000883"
     CDS             complement(20663..21001)
                     /locus_tag="SCK51_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEG1251527.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21003..21359)
                     /locus_tag="SCK51_000884"
     CDS             complement(21003..21359)
                     /locus_tag="SCK51_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEG1251528.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21365..22552)
                     /locus_tag="SCK51_000885"
     CDS             complement(21365..22552)
                     /locus_tag="SCK51_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEG1251529.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22743..23588)
                     /locus_tag="SCK51_000886"
     CDS             complement(22743..23588)
                     /locus_tag="SCK51_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1251530.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23822..24787
                     /locus_tag="SCK51_000887"
     CDS             23822..24787
                     /locus_tag="SCK51_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEG1251531.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25164..26207
                     /locus_tag="SCK51_000888"
     CDS             25164..26207
                     /locus_tag="SCK51_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEG1251532.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26269..26733)
                     /locus_tag="SCK51_000889"
     CDS             complement(26269..26733)
                     /locus_tag="SCK51_000889"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEG1251533.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            26979..27926
                     /locus_tag="SCK51_000890"
     CDS             26979..27926
                     /locus_tag="SCK51_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1251534.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(28137..30209)
                     /locus_tag="SCK51_000891"
     CDS             complement(28137..30209)
                     /locus_tag="SCK51_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEG1251535.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(30142..30519)
                     /locus_tag="SCK51_000892"
     CDS             complement(30142..30519)
                     /locus_tag="SCK51_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEG1251536.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(30648..31226)
                     /locus_tag="SCK51_000893"
     CDS             complement(30648..31226)
                     /locus_tag="SCK51_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1251537.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31358..32650
                     /locus_tag="SCK51_000894"
     CDS             31358..32650
                     /locus_tag="SCK51_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG1251538.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(32710..33828)
                     /locus_tag="SCK51_000895"
     CDS             complement(32710..33828)
                     /locus_tag="SCK51_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEG1251539.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(33842..35263)
                     /gene="arcD"
                     /locus_tag="SCK51_000896"
     CDS             complement(33842..35263)
                     /gene="arcD"
                     /locus_tag="SCK51_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEG1251540.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(35509..36630)
                     /gene="mnmA"
                     /locus_tag="SCK51_000897"
     CDS             complement(35509..36630)
                     /gene="mnmA"
                     /locus_tag="SCK51_000897"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEG1251541.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            37034..37633
                     /locus_tag="SCK51_000898"
     CDS             37034..37633
                     /locus_tag="SCK51_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEG1251542.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(37771..39057)
                     /gene="efmA"
                     /locus_tag="SCK51_000899"
     CDS             complement(37771..39057)
                     /gene="efmA"
                     /locus_tag="SCK51_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HEG1251543.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(39328..39672)
                     /locus_tag="SCK51_000900"
     CDS             complement(39328..39672)
                     /locus_tag="SCK51_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEG1251544.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(39809..40954)
                     /locus_tag="SCK51_000901"
     CDS             complement(39809..40954)
                     /locus_tag="SCK51_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG1251545.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
BASE COUNT        11823 a         8645 c         7189 g        13460 t
ORIGIN      
        1 ataaactacg cgaaaggagt ctaacgttgt ttgatataac ttagactgat cgttaactta
       61 ttttttatat ttctacatag ttatctttag aaccatttca gatacatatg aaacaattac
      121 atatgatttt ttcataaaaa aacagtactg tctagtagta taaaatatac aaatttctta
      181 aaaatttttt cactaaaatc actacctgaa acagtctaaa acacttatat atcaacgttt
      241 aatgaaacta tctccgtttc atttcagata caaataaata tagtcgggct ccaaatccga
      301 aaaaaaatac ccccagtagc ttatcctaag ctactggaga tgtataagta cggtcatata
      361 acaacggggt agggtaaata aatgaaaaag atggaagaat cattattcaa taactttttt
      421 aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat accacagaat aatctttttt
      481 cccattttca agtaaccgaa gtggaatgtc tttaccaact tgctctgtca ctgcattact
      541 gatacctgtt agattagcta ataaatctga taaagattga gcatctgtag aaatctctgt
      601 atcagtattt atcgtgattc ctttatcttc atttacaagc ttccaagtag gtggtatagc
      661 tgtgtatgtt tcgatgaatg aatcaattct ttcatctatc gtcttcccag tatctacttc
      721 tgtctcatta ctcttagatg aatcgattct ttcatccatc gtctgctccg taactgcttc
      781 tgtctcatta ttctcaccgg aaactctctt cacacccaaa cttatatatt gtccatcttt
      841 agtgtgcata gttaaaattc ccacgctttt actaataaat tcactataac cagttctttc
      901 aatagagact tcaatgtatt tcacactatc atactcaaaa gaaaaataga catgtaaaag
      961 cattttgttg tttacatcta cataataatc ctttgactct acttgagacc ttcctatacg
     1021 gaatacagct ttactgtctg atatctctat accagcatcc ttattattaa tctgccaatc
     1081 tccaagtagc tcttctggca agctagtagt ttcttgtgaa cactttacca cttcactggt
     1141 agaggtcgaa acattcacct ccgcactgtt agaagaattt tccaaggaat caggagcctt
     1201 agcacaacca acaagtcttt atatattgtg cagccgatat tttcttcatt cagtacaccc
     1261 ttgaaatatg cagtgttaga taaagcagtt cgtcctgatt tgctgaggca cccatctctt
     1321 cttgaacgta ctccttgatt ttcaaggccg cccgatactc tttcggaaaa cgctgcttca
     1381 ctgaagccag caaaaaatca tccttctccg gcaactcttc cttcttaatc attcgggcaa
     1441 taaaaaactg taaatgcgtc acaaatctta agtaaagcat cgaatcttta tcaacctttt
     1501 tttcgggaaa cagatatcct agcatagcca agatatcctt tagtgctctt accgagagca
     1561 tggtttcttc catatttgct tgttcacttt gggcatttac aatgtgaaac gcaatgtttc
     1621 cagcctcctc ttctggcaac tgaacattca tttttttgtt gatcaattgg atagcatagc
     1681 agccgatctc aaattctttt gggtaatact tttgaatttc aatcagcagg cgattttgaa
     1741 aaacaatgcc tttttgactg cgttcaatgg catgcttcaa gtgatcaacc aaggtaaaaa
     1801 atagactttt attcagctta aagtcaaact tggcgttggc ataatcgata attcgcttga
     1861 cgcaaataat tagatcatcc ggagcagcct caaacagttt aatgtaatcc ttttttagag
     1921 catcagaaag tgaatctaat ttaaatactt tttggatttt gtcagccgga accaaatcgc
     1981 ctttctttag ttgaaatcca atccctttcc ccatcaaaat tacttctttg ccttgcttat
     2041 ctaagcaaag aattaaactg ttgtttaacg ttttaattac tctcattaga ttcaccgttc
     2101 tctctaagct aagccatttc ttgcgattac tttttgatac caaaagaagc tttctttttt
     2161 tattcgtttt aattctttaa gattctcatc ggttcggtca acatatataa aaccataacg
     2221 cttattgaag ccttgatggg tactaacaac atcaatcgct gaccaaggta aataaccaaa
     2281 aacatctaca ccatctgtta aagctgcttg aatagccaga atatgtttgg ctagataatc
     2341 aattcgatcc tggtcattaa ttcgtctatc ttcctcaact atgtcgggtg ccccatagcc
     2401 attttcagta attaaaatag gtaaatcata gcgatcgtac aatttcctca atgttaatcg
     2461 tagtccagta gggtcaacta cccagccata tggcgtttta tctacataag gattttcagc
     2521 tgcccggtag acaccttctt ctcccaacat gatctgttga tcccccgctc tagcggaaac
     2581 atctgatccg tcatttttgc tggcagcgat ggtggcagtt gaataataat taatcgcaat
     2641 aaaatcgggt ttaccggagg ataaaattgc ctgatcctct gcctcaattt taggatataa
     2701 tccacgatca ttcaaatagt tttcaaacaa ataattgtat tttccccgaa ctgctacatc
     2761 taagaaagac cagcctctca atgtttccca attatgggct gcaattgcat cttctggact
     2821 ggaggtagct tgatacatag ccgttaaatt gattgctgga ccgatttttc ctttaagatt
     2881 caactgatga tacaagtgaa ttactctagc ttgagctaat aaaacatggt gattaatttg
     2941 aaagagttct ttttttgaag gcagctctcc acttggtggc aaaccaatcg ctccaggatg
     3001 aaggatcatc gtgttttgct cattaattgt cagccaatag ttcaccttgt caccatactc
     3061 agtaagcaat acctgtacat agtccacaaa ataatcgata gttgctcgat ttaaccaacc
     3121 gccctgctcc tctagaaaat aaggtaaatc gaaatggtac atggtgacaa tcggttcgat
     3181 tccctgattc aggcattcat caattacttg atgataaaaa gaaattccct tttgattggt
     3241 tttattattt tcaatatcca taattcgcgt ccaagcgatg gaaaagcgaa aagcttttaa
     3301 gcctaattct ttaaacagct gtatatcttc tttatagtga tggtaaaaat ccgaggcaac
     3361 ttcaaaatta gctacccctt ctgtatgctg atacttgtca ataatagacg gttgtttccc
     3421 atcctcctga taggcaccct ctgcctggta ggcactaatg gaggcacccc ataaaaactt
     3481 ctctggaaat gctgaaagct gtttgtattg cacaaaaatc ctctccttaa ctaatttgca
     3541 tcaatggttc gtgttccttt aagcttccgc ttgcttcact aattcctgcg taattttcac
     3601 tgttggtcac taccacaatc actgtatcat cgtagccagc gtttctaatg gcagcgaaat
     3661 cggcgttgat taataattgt cctgctgaca cctgatctcc tgcttttaca aatacttcaa
     3721 agggttcccc ttttaaattc acggtgtcga tccccacatg gatcaataat tcaatacctt
     3781 ctttagaacg tagaccaatc gcatgtttag attctaaaat agtaatgact tctccctcaa
     3841 caggtgaata aatgctgttt tcttctggat gaatggctac gccctttccc aatagtccct
     3901 tagaaaagac agcatcgttc acttcagaaa taggacacac ggttcctttt accggatttt
     3961 caacaatgac tgcttcggct attgccgttt gctctgattc atcagaagta tcgtcaaatc
     4021 caattagata agcaatgata aaggttgcaa caaaggcgat tacacacgta ataattgcgt
     4081 gaacaatatt cattggattt tctccaataa aggctggtaa ggctgctaag ccaggggaaa
     4141 caaaggcata tctgactaaa ccgctcaagc cagcatacaa accagcaatt gcaccaccaa
     4201 tcatggaagc aactaatggt tttttcagct tcaaggttac tccatagagt gaaggctcag
     4261 taattcccat taaagcagtg atcccagtgg acacagctag ctgcttcaac tctttgtttt
     4321 tagttttcgc ggctacagca aaggtagcac ctgattgagc aatattagaa gccagcattc
     4381 ctggcccatt gatcatttca gaacccgtac tgctcaattg catagtagcg attggcgtca
     4441 ttgcccaagc tgtacccgta accaccaaaa atggctgcaa accacccatc aaaaatggaa
     4501 ttaaccaagc tgctttgccg ttaatccatt cagcccccat tgctataaga tcgttcagca
     4561 acgttccaaa cggtccaatg acaaccaacg ctaaaggccc cataattaaa aataccagca
     4621 tcggcttagt aaagaattta ataaacgaag gcgaaattcg atccgcaaag ggctctacat
     4681 aggacatgat ccaaacagta aagataatcg gtaaaactga tcccgcataa tctgcagcta
     4741 ataccggaat tcctagaaaa gaaacggcat cgccagtaga aaaaagctgt gaaatatttg
     4801 gatgaagcaa aatgccagca acagtaatcg ccaaaattgg gttgacatca aactttatgg
     4861 ctgccccata ggctaatagt actggcataa aataaaaggc tgcatcagca atagtattca
     4921 aaatgatgta agtttgacta ctgtctgata gtacaccagt caaagtcaag acggccaaaa
     4981 tggcttttac catcccggca cctgttatag ctggaatcat cggtgtaaag gtggtagaaa
     5041 tgacactaat caatcttgaa ataatacttt cttgattgtc tgaagagggg ccctttttaa
     5101 ttgaagttcc cacttccttt tgaatttcct tgaaggctgt ttggacttca ttgccaatca
     5161 ccacttggta ttgtccaccg ctatccacca cactaattac accaagtgtt ttttctaagg
     5221 ctttcgtatc ggctttcctt ttatctttga gttcaaaacg cagtctggtg gcacaatggg
     5281 ttaagctgac cacattttct tttcccccaa cctgttgcaa tacatttctt cctacttctc
     5341 tgtaatccat tattctctct ccttaaagca gtaaaaaaga ccaagtcaaa ccacgcccca
     5401 atcaacattg aagtgttagt ttgtcttggt cttgcctgca taatcagtaa catgccaata
     5461 acgttatttt gctgtaatcc aataatagca cattatgata gcgcaatcaa cagggaattt
     5521 aattttttat cttttcgttt gaccgtatag ggtaatataa cagttagatc acatcaacta
     5581 gggtatatct gcgtatactt actgctaaaa taagtatatc taatattaac tataccagta
     5641 aaaccattcc tacataacca gcgggaaatt cccaaaatcg tgaaagcatc aaaaatgcat
     5701 ataacggatc acacaacttc aacaattctg ctcttgggaa aaattgatga atttggtgag
     5761 taaaggatgg aataaacagc attccaacaa tataggcaac aatagtgata cttgtttttt
     5821 tggtaaaaat cagtaagcaa tagccaattt caatcattcc gagatataaa ggtatcgata
     5881 gaatgatttg ggctagtatt cttaaaaatt aactagcaaa tgtttaaact gatttatttc
     5941 taatctacat gtagtaagta tttcaattcc accaattatc aataaaaata ccaaacatat
     6001 ccataataat tttttctttt tcatttcttt tatattgtta taacagtgaa taacataaaa
     6061 attcccgaca atcattgtaa tatttttttg tcacctgtat tagtatttaa tttataggaa
     6121 actataaaaa ctccctagtg aatttacacc tttgggagtt cctgtttgga cggtatgcat
     6181 aatacattta aatcaattta acattaacac ctcagcatat ggtatctcca catatatacg
     6241 gtctttcttt ggatagtact caatatttat gttgatagca cttccattgt ctaatatagc
     6301 tttagcccgg tatgtgttat ctccattttc atgagtaatc gtcacatcat cacatgatcg
     6361 atttaagttg tactgttctt gtaaaatctg tgttaccaat ggttttgatg acttctctaa
     6421 tccagatgta cctgtcattt ttgaaaaaat aatacctata atagccaaga caacaatacc
     6481 tataatgaca aacttcttgt tcatatttac tttattcaac tttttatctc ctatctccaa
     6541 aataatttgc tcttatccct cttatcagta cattgacatc cctaaaagta caccaaagat
     6601 tgctccagct acccctgcta tcatcaaaat cgtacccatt ttctcatctt tttctcgata
     6661 taagtagccc ataataacag caacagcacc aaatacaaca ggaataaatg ctaacgatac
     6721 taccatagaa atccaaccaa taatcaaata cagcttccct gtctcttgtc catgtacgat
     6781 ttgttccttc atttcattaa ttgtctgctc agtacttttc tttactgtgt ctgttccggt
     6841 tgtctcatct gtttcttgta gtgctccaca aaactggcaa tacttacttt catctgttat
     6901 ttctttgcca cattttctac aatacatgtc ttctcctcct aaaatcattc aattgcttat
     6961 tagttaactg tttactttat taaatacatc agaaaacagc tcattcattg gtacacttaa
     7021 tatctcagaa agcatcttca tttgataggg tttaggaatc gttctccctg tttgccacga
     7081 acttacacat tgctgtgtaa cattcatttt ttttgctaac tccgtttgag aaagtccttt
     7141 ttcttttctg tatcggctga gattacttac ttttttcatg attctcccct cctgttttat
     7201 tatatacaac aatatgatgt atttcaactc ataaaatata aaaaacttca aataccatta
     7261 caaatacaac aacttgttgt actatactga gatgggggtg tattaatgaa acagaaacaa
     7321 tatgcaaagg tcattagaga aaagcgaaaa gctctcgggt ggacacaaaa agaattagct
     7381 gaaaaaatct tctctacaca acaggcagta gcaagatggg aaaattctgt aacagagcct
     7441 aacctcgata gcttaacagc tctttctaga gccctaggaa cccctgtaag ccattttcta
     7501 gacaatgttg tcgtagatta tgaggaagag tttttggctc tctatcgttc tcttagcact
     7561 gaagatgcag ttcgaacaat tgactatatg aagctattaa aaaggcaaga aaatgaacgc
     7621 aatcagctat tgaaagattg aaaaataaat aacccaccga actttaacgg tgggtgtatt
     7681 gagatgaaat aagattaaga gctatggata acctgtctag tttaatacaa ttgggtcttt
     7741 tctcttcctt actctccgtc catcatctct tgttacactt gtaaattgat acagaggaac
     7801 tatcccagct cctgataata tcaattcttt tattgtttca tctatccctg cccctttcgc
     7861 taaatatatt aaaaaactac tacttgcttt cacattgtat agtttcttaa tatccttaga
     7921 agctcgttca agagtgaatc cttgtttaac aaagtagcct ctgtttttta ttaaagcatt
     7981 tttcacgctg ttatccccaa cattacgtag gcgttcacaa acagtgttaa taagttcttt
     8041 ctctgtcatt agatatgtcc cttgccacga taagccctgt aaaacttcat gtgcttcatc
     8101 taaaatcaat tgtttttcct tgctgagttc tgatcgggaa ttttgaggaa atactctttt
     8161 agcctctttt tcgttaaaac atatcttaac ccattcataa gaaaaatatt gatatgtaaa
     8221 atgaattttt tccagaagtg gaaggatatc ttcattcaat ttttgaatca cagaagttgc
     8281 attaaaaacg tctaatcgat agacattgct tctcttatga ttaaagtttc ctaacgcttt
     8341 ctcttccttt aacctattta ggtattcgag catcttcaaa ggaacatctt catcatctaa
     8401 tttttttatc aatcctataa aagtcataag tgatagtaac ttattacatt tatctatgtc
     8461 ataagtttta ccatagtaag aactcatttt tttagctaat tgttcccccg acaaaataca
     8521 agtcacactt tccttactat catcaaaaaa attaacatca gaaagcaaga catcatagac
     8581 acgtcgcatt tcctctagat aattccttat tgtcctgaaa agtttgtctt tcattgattc
     8641 aagagtgtca aaaagtactt ttgattcctc tttcaatttt cgaaaatgtg taggatacaa
     8701 gtctaggttc tctaatagcc aattaaatgt ttctacccga ctgtctttct ttaaaattag
     8761 catcacaact ccaataaaat tttcactgat atcacaatta ctggaatgac aataaaaaat
     8821 ggaatgccca gttttactgg taaaaatact agcagaggga ttatgatcgt tgtgaaacag
     8881 acatcttaag tttttacttg acttaagtaa tttattcata tcaatggtta acaatctttc
     8941 ataaatctca ttaaaagaca tgtcggagga atcaaacagg cagtctttaa accactgttt
     9001 catttcttct ttgtcgtcat tttttatcaa cttaacaaaa tcggtttcat tgactagttc
     9061 atactctctc cctgaatcac tttgacaatt tttagaacat gtcatggtaa aatcaatcgg
     9121 aagctcagta atatctaaca tgttgtcttc attaattatt atcactgcat ctgacttata
     9181 tcctccaaaa aacattctat tcgaagcatt tacattgtca tcacacccgg ggatgttctt
     9241 tttcaagtat tcgtaaacag caaccatttt accatagtca gtaattgctt cttttaatag
     9301 aaagacaatc ttgtattttt ctagattttc agagtaccga atactttttt gtatcgcaaa
     9361 cgcagaatta tgaactaaat tgatagataa aaccttttct acgcttaagt atctactatc
     9421 atctttactc aaatctacta ccttcccctt ctcatcttta acagtgttat cgacatccag
     9481 aataattact ctggtttcca caaaactctc cttgtcattg cctttattct ttgtcttgct
     9541 tggaataaat gattgtgatg aatttagtat ctctacaaac tcatctcttg taacttctct
     9601 atagttatct aaacacctat ctcttacatc agttttttct ttttttgtaa ctggcttctc
     9661 taataatttt tcttgatcta cataaattgg aatattaatc attgatgttt ccatttgtgt
     9721 ttcctcctta taaaattctg ttaaagacta aaacatacag ataataaaaa caaactcttt
     9781 attactatat gttttagtct tatttgttta ctcaaccttt ataccgttcc atgaaactat
     9841 caatatcaga ttttttaaat ctaatgaccg tatcaatttt tataacaggt aacccgtatt
     9901 gagaaacata ttttctaatc gttcctactg atacacctag atattcactt gcctgctgat
     9961 agtccatata ctctagagtg actccagaaa taatgtcttc tagcataggc tttagcactg
    10021 taattatctc tttcacttgt tgttcaactt gtatagaaac aatgctatca atgatttgta
    10081 gagacatatt tccccctcct ttcgtgattg cttgaacaaa ttttctaaga attatcttcg
    10141 tattttttgc tcaaaataaa agtattttgt tattaaccga ttaatcaaac aaaaaatgat
    10201 taccagttca tcgacataca cataatacct cgataaaaaa ggggtgtcaa cagctttgta
    10261 aaatttattt gcaacacccc ttttttgagg tatactaatt ttaggaggtg tggtcaatat
    10321 gataaaacgt ccaccaataa attatttaga acgaaaaaag atactcggaa caaaaataaa
    10381 agctataaga aagagtaaaa aattaacaca accagcattt ggtcttatga ttaacaacgg
    10441 tcaattaatt gacaaaaaaa ctatctacga atgggagaag ggaacctatt taccaatacc
    10501 agagcgatta tcccgaattg ctgatttggg aaatatgagt atagaggagt tagtttgtgg
    10561 caatgtagaa gaatatattt tgggaattat tctttataga gatagcattg tactagatgg
    10621 tataacattt ccagataaaa acctgttcca gcatcttcgt caacagttcc caccagtaca
    10681 ctctaattta gatacatggc tggatagata ttccaaatta gaaccagaga tgcaagaatt
    10741 tattgctaac aagacttgta ataaagttaa aaatgaaaaa attagtcttt tcaatatctt
    10801 aaaaatagaa gaattattca tcaatgctat tgtagaagaa tttgacaata acattctttt
    10861 cctcactagc agtatagaag aactactaga aagaatggta gatgaatggc tacctattca
    10921 attgaaagac atgtcttatc ccgaagaagc tgtgagagaa attactgata atataaacaa
    10981 gctagaacaa acgatttcat caattgggaa aaaatacact aagaaaaaaa tgaagggagg
    11041 tgataccatc tagtggtaag tactccttgt aaactaagag gagatgattt aatggcaaaa
    11101 atatcaaagt acaaaaagaa aaacggtcaa gtggcgtgga tgtataaagg acacattgcc
    11161 accgatccac gaactggtga aaaaataaat actacgagaa gaggttttag tagcaaacaa
    11221 gaagctcaaa aagattatga cgagtacaga catcaaatat tatacggtgt taaaaagaaa
    11281 gcacctgata tgacatttga agacctgtat aatgaatgga taactcatca gcgtaccagt
    11341 gttaaagctt caacaatcgc aatctcagta agatacgcaa ataatcaaat acttccagca
    11401 tttggtaagc ttaaactatc gaatatctct gtcccatatt gtcaaaaagt ggtagatgaa
    11461 tggcacagta aatatgaaag ttacgattat atgagaaaac agactgcaca aattttacgc
    11521 tacggggttg caatgcaata tattgacaat aatcctatgg aaaaaacact tcttccacga
    11581 aaaaaagagt atgagaaaaa tcgtaaattt tactcaaaag aggagcttaa taatttactg
    11641 gatgctttta aagattttgg taacatgaaa caatacgcct tcttcagatt attggcatac
    11701 acaggtatga gaaagagtga agtactcgct ttacagtgga aagacataga cacgtttaat
    11761 aaagaactac atgtcaataa aactcttgct gtagatgaat ttggtaaagt tataattcaa
    11821 tctccgaaaa caagagcttc tagacgagtt atctctttag atactgagac tctttctatc
    11881 ctcaacaact ggaaattaca gcaaaaagaa gagtatttaa aacttggata caatactagc
    11941 tctaaagagc aacatgtttt tacaacagtt aaaaacactc tatacattcc aaacactgtt
    12001 aatgattggt taagatatat tttgaaaaaa tataacttgc ctcgtattac acctcatggg
    12061 tttagacata cacatgctag tttattatta gaagcaggag aatctgtcaa ggtagttcaa
    12121 caacgattag ggcatgaaaa tagtaaagtg acattagaca tctatgcaca tattacaaat
    12181 aatgcaccta agaaaacagg tcaagacttt gctgatatga tggctcatca gtaaaagagc
    12241 tggtcaaaat ctggtcattt ctagtataca ggcaaaacaa aaaagctaga acccttatat
    12301 atcaagggtt ctagctccta taacaggttt acccaacact gccttccatc tcataactga
    12361 tcaatcgatt gagttcgact gcatattcca ttggaagttc tttggtgaat ggttcaacaa
    12421 agcccatgac gatcatttca gttgcttcag attcggtcag gcctcggctc atcagatagt
    12481 agagttgttc ttctgagatt tttgagactt tggcttcgtg ttctaaagag acttggctgt
    12541 tgtggatctc attgaatggg atcgtatcgg atttcgacca ttcgtccatg atgatcgtgt
    12601 cacattcgat atgagagatc gaacccgcac tgtttttggc aaaggttact tgtcctcgat
    12661 agtttacttc tccgccgtct tttgaaattg atttagagac gatagagcta gatgtatttg
    12721 gtgcattgtg gatcattttt gcacccgtat cttggatttg gtttgctcct gcaaaggcga
    12781 tggagagcat ggtgcctcgt gcaccttttc catctaagta aacacttggg tatttcatcg
    12841 ttgtttttgc acctaggttt ccgtcgatcc attcaacggt tgcgccttcg tatgcttttg
    12901 ctcgtttggt tactaagttg tacacattgt ctgaccagtt ttggatcgtt gtgtaacggc
    12961 aatacgcatc ttttcttgta aagatttcta cgatggcggc atgcaggctg ttacttgaat
    13021 aagtaggtgc cgtacaacct tcgacatagt ggatgcttgc tccttcgtca acaatgatca
    13081 gcgtacgttc gaattgcccc gtgttttccg cattgatccg gaagtacgtt tgtaatggaa
    13141 catcgacttt gacgcctttt ggcacgtaaa taaaggtccc acctgaccat acggctgaat
    13201 tcagcgctgc taatttgttg tcggttggcg gtacaagttt tgaaaagtac tctttgaata
    13261 attcagggta ttctttcaaa gcagagtcag tgtcagtaaa gacgattcct aacttttcga
    13321 attcatcttt catattatgg tacacgactt ctgattcata ttgtgcggaa gcaccggcta
    13381 gataagctcg ttcagcttct ggtataccaa tcttttcaaa tgtatctttg attttttctg
    13441 gtacgtcttc ccagtcacga gcgggtttgt cacttggttt ttggtaatat ttgatggcat
    13501 cgaaatcgat gtctgaaagg tcggggcccc atgtttgcat tggcattttg ttaaatgttt
    13561 ctagagattt cagacggaat tcaagcatcc attctggttc gcctttgatt cttgacatct
    13621 cacggatcac ttcttctgtc aagccttctc ctgtactgaa aaccggttcg acatcatcgt
    13681 gaaacccgaa tttatattct tctaattctg gtacgcccat gttctcactc ctcttccttg
    13741 tggtaatgat ggaaagctgc ggtaccatcg ttttccattg cttttcctag tgctttccag
    13801 gcaagtgtcg cacatttgat ccgagcggga aatttagcta caccaccaag catagcagca
    13861 tcgcctaatt ggtcgggatc tttgacttct tttccttgga caagctgtag aaagtctaca
    13921 gttaaatgtt ctgcttcttc tattgatttt ccgatgacgg cgtctgtcat catcgaggca
    13981 cttgccgtac tgatggagca gccactgccg tcaaaggcga tatttttgat gattccgtct
    14041 tttacatcga cttgcaattc gatcacatct ccacatgtgg ggttgttcaa ttcgatcttg
    14101 cttgtcgagt cagtcaagct tccacgatga tgcggatggg aagaatgatc caagatcact
    14161 tgacggtata aattgtctaa tttagatagt cccatgttgg aaaaactcct ttgttgccat
    14221 gattgcttca actaaacggt cggcgtcttg cgttgtgttg tagaaataaa agctcgcgcg
    14281 agcagtcgct gatacttcaa ggtacttcaa tagcggttgc gcacaatgat gacctgctct
    14341 gacggctacc ccttccatgt ctaaagctgt cgcgacatca tggggatgta acccatccaa
    14401 attgaatgca acgactcctg tccgaaaatt cgggtcttgt ggaccataaa cagtcaatcc
    14461 atcaattgct tgtaattttg gcaacacata agcggctagt tcttcttcat atcgatggat
    14521 attgtccata ccgagttttt gcaaataatc gattgcatgg cctaatgcga tagcgccggc
    14581 gatatttggt gtccctgctt cgaatttcca aggtagttct ttccatgtac tgtcgtataa
    14641 ttcgacaaaa tcgatcattt cgccaccaaa ttcaacaggt tccatttctt ctaaccattt
    14701 tcgtttacca tataaaacac caatacctgt tggtccgcac attttgtgtc cgctgaaagc
    14761 aaagaaatca cagtctagtg cttggacatc aatggccatg tgcggggcag attgagcacc
    14821 atctactacg atgaccccgc cgttttcatg gacaagttca gctaattctt gaacaggatt
    14881 gaagacacct aagacattgg atacatgagc aatggagacg attttggttt tgtccgtaat
    14941 ctttttcgct gcatcctcca tatccaggaa gccttcttct gttagttcga tatatttcaa
    15001 gacagcacct gttcgttttg ccaactgctg ccacgggata acattagaat gatgttccat
    15061 gtaagagagc actatctcgt ctcctgcttt gatatagcgg tcgccgaagg ttctggctac
    15121 ccaattcaag cttgtcgtcg taccacgagt aaacagcact tctgctgttt cttttgcatg
    15181 gatgaacgcc cgtacttttt ctcttgcttg ttcataatca tgggtcgcgc gttctgctaa
    15241 cgtatggaca ccgcgatgaa cattggcgtt atcgtgttcg taatagtacg ctaatgcctg
    15301 gagtacttgt tttggctttt gggtcgtcgc tgcattatcc agataaacta aaggttcatc
    15361 attgacgtct tgaaataaga taggaaaatc attgcgtagc tttttagcat cgatcatcca
    15421 ttcaacttcc cttcgattac ttcgactaat tctttccgaa cttgttctac agggattgct
    15481 gttaagacag aaccgaggaa tcctcgaatc actaagcgct ctgcttcttc tttatgcaat
    15541 cctctactca tcagatagta catttcttct ggatcaactc gtcctacact ggcagcatgt
    15601 ccagcagtta cttcgttttc atcgatcaaa agaataggat tcgcatcacc acgtgcttta
    15661 tcagaaagca tcaagacacg actttcttgt tgggcatctg caccttttgc ccctttcaag
    15721 atgtggccga taccattaaa ggtcaatgtt cctttttctc ggatcactcc gtgttgcaaa
    15781 atatgcccga tcgtatgagg ggctttattg gttactcgtg tatcgattcc ttggatttgt
    15841 ctgcccgagc tgattgccac gattttgact tctgcgtgtg caccttctcc tgctaaatct
    15901 gagtcaaagt cagcgatcac atgaccgtca ttcatgacac caattgccca gtcgacagaa
    15961 gcatctcgta aaatgtgccc acggcgattc atataagaag taatattttc tcctaattga
    16021 tctacggctg aatatttgat ttttgaacca gctttagcga tgacttccac gatgatatta
    16081 cctgatgatt ttgctacttg ttctttggtt gtctggaaac gttccagata agaaaattca
    16141 ctatgttcat ctgccacgat caacacatgc ttgaagaagt gctcatcgga tgcgccatct
    16201 tgaatgaata aggactcgag tggttcatcg atcacgacat ttttaggcac atacaggaaa
    16261 ataccgctgt tcataaaagc tacatggagt gcagtcaatt ggtcttcgtt catgtttact
    16321 gcttttttca tataatattc ttggactagt tctggatatt cgatcattgc agtgaacaag
    16381 tcggtaaaaa tgactccttt ttctgctaat tccacaggca gttgttcaaa aatcgtccat
    16441 gaaccttgtt ggacaagcac aggattgtct ttcattgcat caaatgccgg aatcgtgcct
    16501 gtttcactga tggtttgtgt ttctttcaca tcaaataacg accagcgatg gaatttcacc
    16561 cgatcgatat gcggcaatgg cagttcatct gctttcgcta gtgcgtcttg acggaaggtt
    16621 ctcatccaat caggttcgcc tttacttaaa gaaaaatcat ttaccgcgtc aagataatcg
    16681 agccagtttt tttctttcat tcttgctccc ccttacgctt cttcttcttt atagtcgatt
    16741 cctaattctt tactgatacc agcgtagcct tctgcttcca gacgtttagc taggtcagca
    16801 ttccctgtca tgactactcg gccttccatc atgatgtgaa cgacatcagg tgtgatatag
    16861 ttcaataatc gttgatagtg ggtgatgatc aatgcaccga aattgtctcc gcgcatttca
    16921 tttactcctt tagcaacgac tttcaaagca tcgatatcca gacctgaatc gatttcgtct
    16981 aaaattgcaa atgtaggttc taacatcaac aattgcagga tctcattccg tttcttttct
    17041 ccgccagaaa aaccttcgtt cagataacgt tcagccattt cttctggcat atttagtaaa
    17101 gccatttttt catctaactt tttcaaaaat gccataacag aaattttgtc gttttcatca
    17161 cgtttcgcat tgatcgctgc acgcatgaat tccgcattcg tgatacctgg tatctcactt
    17221 ggatattgca tagctaaaaa taagccaagt cgcgcacgtt catctacttc caagtctaaa
    17281 acatttttcc cgtcaaacaa aatttcgcct tctgttactt catagtttgg attgcccatg
    17341 atagcggcag ataaggtaga ttttcctgtg ccgttaggtc ccatgatggc atggatttct
    17401 ccggttttca ttgttaggtt tacgccttta aggatttctt tttcctcgat cgacacatgt
    17461 agatttttta tttctaagac agacatgcat tataccccca aattctcttt cgttctcact
    17521 cattttagac taattgagaa tgaagcgcaa tgaccttcct tactaaaatc aaatatcttt
    17581 tatttattat taaacaattt tgatttctaa attagaatag gtataaaaaa gatgagaaat
    17641 aagccattac agacttattt ctcatctttt ttgttccata ccttattttg catcagcctt
    17701 tactggttta acagagccgc cccatttgtc tgtgatccat tcttggactt ctttgcggtg
    17761 aagcacttca atcagttttt tgattttgtc actgtcttca tcacctttac gagtcgcaat
    17821 aatattcacg taaggagagt tgtccttttc aagcaatacg gcatcttcta atggattcaa
    17881 ccctgcgccg taagcgaagt ttgcgttgat tgcaactagg tcaccttcat cattttggta
    17941 cgtagaagct aaaagtgatg ggtcgatcgt atggttgaac ttcaagttct tagggttttc
    18001 ttcaatatca tcgaatgttg ctgtttccag atctactcca tctttgactt tgactaagcc
    18061 agcatcttgt agaattgtga tgatgcgtcc ccagtctgat tcagaatttg aagtaatgat
    18121 tgttgcacca tcttttaatt cggaaacatc tttgactttt ttagagtaca agcccattgg
    18181 ttcgatgtgg acagcaccgg cattgacaaa gtcatatcct ttttctttga tttctttgtt
    18241 caaataagga atatgctgga agtaattggc atcaatgtcg ccttcttcca aagattggtt
    18301 cggtaataca tagtcatcga atttgacgat ttcaagatcg attccttcat ctttcaataa
    18361 tggtttcaca tgttccaaaa tctctgcgtg cggtgtcggt gaagcgccga ctttcaatgt
    18421 cgtgtcgtct gctttatttg ctgaatcttt gctgtcgctg ttgttaccgc atgctgctaa
    18481 tcctacagtc agcaaaagcg ctgctgcaaa tccaaaaatt ttctttttca tatcctacat
    18541 ccccctatat cctattatct tttgtctgtt ttcttagtta agaaatcacc gattccttga
    18601 acgataaata cgattagtaa aataattaaa gtcgcaacga atgtcactgt tagattacca
    18661 cgctggtagc cttcctgcca agcaagacct ccaagtccac ctgcgccgat cgcgccggcc
    18721 atcgcagtaa atccgatcat cgagatcgtt gtaactgtta atccagaaat caatgcggga
    18781 atactttcag gtatcaagac cttccagatg atttcccagt aacttgctcc cattgcatct
    18841 gctgcttcca gtacaccagg gctgacttca cgaaaagcaa tctctactag acgggcataa
    18901 aatggtgctg cggataaaac aagagctgga atggctgctt gcgcacctag catagtgcct
    18961 acgattgctt tagtaaatgg aatgatcaaa accatcagga tcataaatgg cgttgaccgg
    19021 aaaatattac tgatgatcga tacgattcca taaatgattc gagcatacgg tttgtttttg
    19081 cgtccaatag agtaaaggat caatcccaaa actaatccga tgatcgtcac taagaccatt
    19141 gagattaatg tcatatatag tgtatcaagg atagcctgat tcatcgtttc cgggttgatt
    19201 tgtttaaaat ctagatatgt ttctgaaaag cttttatcca cgatggatca cctcaattcc
    19261 tactccttgt tctttgatga acacttctgc ttgatctaat tctttggttt caccagtcat
    19321 caagacagtc aatgaaccaa aggaaccttc tttcacttgt ttgatctttc catacacaac
    19381 gttgacatcg atgttatagc gtcgaatcgc ttgggaaatg actggctcgt tggcatttct
    19441 ttcgctaaag gtcaaagttg ccaaacggcc gcttggattt tctttgatga gttcagctaa
    19501 tagttctgtc gaatcagctt ctggttcgat gtcttgttgt acaaagcgtt tggttacttc
    19561 ttttttcggt tgtctgaaga cagtcaatac gtctccttct tctacgactt tccccaactc
    19621 catcacagcg actttgttgc agattttgcg gatcacattc atttcatgtg tgatcaagac
    19681 gatggtcaaa ttcaatttct tattgatcgc taacaataag tctaatactt cttctgttgt
    19741 ttgcgggtcc aatgcgctag tagcctcatc acaaagcagg atctcaggat catttgccaa
    19801 tgctcgtgcg ataccgacgc gttgtttctg tccgccggaa agttgtgaag gatatgcttg
    19861 tccgcgtcct tctaacccga ctaaacgcaa cagttcttct gcccgttctt tacgtttgct
    19921 ctttggtacg ccagctaatt ccagcggcag ctggatattt tcaagtacag ttcttgacca
    19981 taacaggttg aaatgctgga agatcatacc gattttcttt cggaatgtac gtaattcttt
    20041 gttttttaat tttgtaatat cttgatcgtt tacgatcact tcgccgctcg tgggaagctc
    20101 aagtccattc aggagacgga ctaatgtact ttttcctgcg ccagaatagc cgacgattcc
    20161 ataaatatcc cctttttcaa cgtttagtga aacgtcattg acggcttgga tgtttccgtt
    20221 tttcccattg aatgtctttt tgacattcgt tagttggatt aatgacatgc cattcacatc
    20281 tttctcttat gatttttcaa acaaaaaagc cttcgccctc ttgccttaag caaaaggacg
    20341 aaagcttgaa cttccgtgtt accaccttga tttaccgtta cttcgcaata acagcctctt
    20401 ccagtacttg gacttagctt ctttccgtat actgtcgcgc tgtaacgggc actcccgtag
    20461 taagtttgct tacgctcgct tactctgctc taagaccatc ttccatctca ttttcgttac
    20521 ccattttcag caaacagggc tctcttcaaa cgtccgtgac atgtactctt cttttcaaag
    20581 ctttctcgtt tcatatagga ttgcttatga ttatagcaac tggaagaatc agtgtcaact
    20641 tttttcaagt gattttctga ttttataatt cgtcaaattc ttttggatca acatctttga
    20701 aacttaagat ccacgcattc atttcatctg aatcattcaa gatattgata tcttcaccga
    20761 ttttatcatt aacagaagaa actgtacccg ttagcgggct ggcaaattca ttgaccgctt
    20821 tttccgcttc aacttcgatc agaggttcat ctttttttag cgattgtcct gcttttggca
    20881 gattggcaaa cgtgatgtct ccaagttctt cttgcgcttc atttgtcaaa ccaacacagt
    20941 attcttttcc attaaataag atccaaaggt tgtccttttt cttcaaatgc ttgcctgcca
    21001 ttttatatca gttctccttc ataaattttt tcattgaatc caattgcaac aagctggtcc
    21061 tctcgtaaga tcagtggtcg cttgatcagc ataccatctg tcgataagac ttggctcgct
    21121 tttgaagccg taaactcgcc tacttgatct ttcagtccaa gttcccgata tttcatcccg
    21181 cttgtgttga acaggcgacg gattggtact tgactcgttt ctagccaagt ctcaatcgtt
    21241 gaggccttcg gtacttcttt gatcatatca acggtttgat aactaacgcc atgctggtct
    21301 aaccacgctt tggctttttt gcatgttgaa catttcgggt accagtaaaa ggtgtacaat
    21361 tttcttacct ccttgaagat cttgcaggtg cttcattttg gtatctcatc gataatatca
    21421 acccgatacc gatcatgttc cctaaaatcg aggatcctcc ctgactaatg aaaggcaatg
    21481 ggatccctgt taaaggaagt aatccgatat ttgctccaat attctcaaag acatggaaca
    21541 gcagcatcat gatcaaaccg cttgcgatat aggcgtaaaa ttcattattc gtatcgaaac
    21601 acacccgaat catacgatag attaaaataa aatacaagaa aatgacgaat gcaccaccga
    21661 taaagccgaa gttttcaccg ataacggtaa aaatcatatc ggactcacga acaggcacat
    21721 agatattgct tacgttgaat cctttaccaa acaagccacc ggtaccaatt gccaaaagtc
    21781 cttgggccgg ctgataactc attcctgacg tatcatggaa agggtccagc catgaatcaa
    21841 tacgtgcaaa ctgataagat ttgaatccaa ctttataaag caagtctctt ccgccttcag
    21901 tcgtaaccaa gaagatcgtt cctgctccaa cgactactgc caagattact acaggtgcga
    21961 tgatgcgcca agagattcca gacatcagga aaatcccgcc aaagatcgca agaaagacaa
    22021 gcatcgttcc aaagtcatct tgggccatga tcaatgctaa aaccggtatt gtcacgatca
    22081 agagcttccc aatcagccaa aagtccgatt tcatcgtccg ttcttttact tgcgtattat
    22141 gtttagttac gatcagtgcc atcatcaaga tgtacgcaat cttcatcaac tctgctggct
    22201 ggaaagtaaa cgcaccgaat cgaaaccagt ttttagaccc tgttgaagca gctaaattct
    22261 gatcatagaa tttgagcaag agtcccatta ccactaaccc caaaccataa aggtaaggag
    22321 tcagtttcca gatccatttg gaattcaaat gcatgatgac aacgattgct gccgctccaa
    22381 ctaaatacca gacaacttgc atccctacgc ctttcaagac tgctggattg ttcggatcat
    22441 ggctaagtgc tacatataat gagagcagac caatcaaaca taacagaaag actggtaaaa
    22501 tcacgccata atcaatacga ttatcgttcg aagcttttaa ttttttttcc atgtttcatc
    22561 ccttttctta aaaaccatac tgctgattat tatagcggtc cagttagata atggaaagtc
    22621 tttccataga catttgggaa aaattaactt tccgtttgtg cagttgcttg agcaaatgca
    22681 gtacaaaagc ctactgctta tctttagtgt atcatagaac tttttttgat acatgcaatt
    22741 atctaccagc tgagattctc ctcgctgtga gaagtttctg tgctttcatt gtatcgaagg
    22801 atctcttcgg caatctctag cagctgatca gaaaccagat tggttcgttg gatgaaataa
    22861 aaatatcgat ggaaattcaa cggagtcatt ggcacatttt ctggaacaga gcgcttagac
    22921 aaaatcgagc gaccgattcc ttccttcagc aacgcaacaa ttacatcgtt acttttcacg
    22981 atcatcttct tatcttggat attcatctct tcaaaataac gctgcgtgta atgaaaaaca
    23041 ccagagtttg cttctcttac aagccacaat ttgctttttg ggtccccagc gatgactagc
    23101 tggtcttcca tcaaagagta tctttgtacg ccggaagtct ctaatggctt ttcgataaaa
    23161 ccaaaatcgg attcatgttt cgctacttgt tctaatgatt catgagaatt cgtgatttcg
    23221 atctcaaaat gcactttagg aaaccttttg atcagatgtt tcatcaactg aggcaaaaga
    23281 tagacagcaa aggtgtggga agcaacgatc cgacatactt cttcttcatt ttgttcattg
    23341 agcaattttt gctgagtttc ttcccattct tctaacaaaa ccagtgtccg ttcatataat
    23401 aagtcggctt gcttagttgg aagcatctct tttcttccgt tacgttgaaa aagatcgata
    23461 ttcaattccc gctccaactg tttgatttga ttcgagactg ctggttgaga taaaaacagt
    23521 acttctgctg ctctggaaaa atttcttgtt tcatatactg ctcgaaaagt cgttaataac
    23581 ttaaacatgg caccacttcc ataacttttt atgataatta ctataaaaac tatttgattt
    23641 aaatatacaa aaaaccatct ataatagaaa tgtacaacaa aacagttgct gatccaacta
    23701 gtaacttaga tgaagacaat cagctgtctg cttgctcgta atcatacatt cctattcatt
    23761 ttacaatgta ttcgttcatc ttggtactaa aatctattaa ataaaaagga gaatattata
    23821 tatgaagaac tttttgaaaa ttattccgat cccgatctgc gggttgatcc ttgggttagc
    23881 ttcccttgga aatctgttta aagactatca ccatgttgct ttaggcaatc taactggcgg
    23941 tatctctatg attcttatga tattcatttt tgttaaattg tttatcttat tcgaacacac
    24001 gaaacaaacg ttgcatgatc caatcatcgc ctccgtttca ccaactttta cgatgtcact
    24061 catggttatt tgtacttatt ttgtttcata tgaaagtatt gcaccaattg tcaaattcat
    24121 ttggctgtgc gctgttatct tccaagtgat ccttgtcttt tatttcaatt atcaccatgt
    24181 tatcaaagct gatctttcaa ttgaagcaat ttatccaagc tggtttatca tttacgttgg
    24241 atttggggtc atcactgtaa ctgccgggaa cttcttccca cttattggaa aaattttctt
    24301 ttggatttca ttgatctgct atgcgttctt attgccgatc atcatcaaac gtgtcttttt
    24361 cgtaagaaat atggctcatc cgacacgtcc gctgattacg atcgttgcag cacctggttc
    24421 tctttgccta acaggttatc ttaaaaactt tgctgaacct aatttttatc ttgtcgtagc
    24481 attgttcgct ttatctcaag tattgtattt cgttgtgctg tctatgcttc cacgtttgtt
    24541 caagcttcgt ttttatccaa gctatgcagc ctttacattc ccgcttgtta tctctgcaac
    24601 agctatattt actactgttc attacttcaa cagtttaggt atctatagta gcgtattgga
    24661 tttcttaaaa gttgcggaat gtctgtttgc aagtgtagtt gtcttctacg ttcttgctca
    24721 ttacgtacgt ttcttaatta aagaacatag caaacatgga aaatttgttt ctaaaaaaat
    24781 cgcttaataa tcgattcaat aggttgtgaa aaagctgttc tgcatcaagt aataagaacg
    24841 gccaaacaaa aacagctgtt catgtttttg tactggttgg gcttattacc gcagatgcaa
    24901 gcttttgaac accgtttacc aagaggtcgt aaaagaagtg gtcagcttca agaaataaga
    24961 aggaatttac gaaaattgct tctcaaattt ttgtgaaatt tcggcttatt tccgtagaag
    25021 ctacttctgg aacaccgttt attcgaaaca agaggtcgta caagactttt gtcacgacct
    25081 cttgttcttt tgtcaaaata ctggtttagt gctactatgt tcgagtatgc ttttagtatt
    25141 tattgacgaa aggaaccgat caaatgaaat cattgtcaca tccaataaaa tctgtaaagg
    25201 attcagcggt ccgcgaaatc ttacccggtt tactgctttc ttttgccatc gctgccttca
    25261 gcaggcttct tgctatttat ctgccacagt taggtggagc gactattgct attttattgg
    25321 gtattttatt aggaaatact ttttttaaac agcctgttct agcaatcggc acaaaatttg
    25381 ctgaaagcaa actattagaa ttctctgttt ttttactagg tgcaactgtc acagctcaaa
    25441 cgattgctca aattggtgta aaaggaattg tttttgtgtt actgcaaatg tcagtcacta
    25501 tcatcggtgc ttacttgatt ggtaaaaaac tgcaattcag cgatacttct tctttgatga
    25561 tggctggagg aaacgctgtt tgtggttctt ctgctattgc ttcaatcgcc ccttccatcc
    25621 atgcgaaaga ggaagaaaaa ggacagatca tcacactagt caatctactg ggtactgtga
    25681 tgatgttgac tctccctttt cttggattac agttgtttgg tggaaatctc attgaaaaaa
    25741 gtgcactttt gggtggtaca ctccaatctg ttggtcaagt agtcgctggg gcaagcttac
    25801 ttgacaattc tgttgtcgaa ttcgccatgc tatttaagat tctacgtatt atgatgctcg
    25861 ttgttgttgt ctatctgttt gaaaaattca tgcacaagaa acaaccagct ctgtttgaaa
    25921 cgccaactga tacgcagaaa aaacgaaaat taccgcttcc ttggtatgta ttagggtttg
    25981 tgatcttttg tttggtcaac agcttgtttt ctttacctgt agttattgac caatcggcac
    26041 attttatcag cagctggttt gagatcactg ctttagcggc aattggtctg cgtttagact
    26101 ttgcaaaatt catgaaagaa ggtacacgct tcttatctta tggattagct gtcgggcttc
    26161 tccaaacagt gatggcttta ctatttattt ggctgatcaa agtttagaat gaacatgaaa
    26221 aaacagttgt gattctcgca tggaacaatg tccgaatcac aactgttttt aattttgcag
    26281 atatccttct aacaacaaca attggcgttt gatttcaacc ccatctattg cgtttcccat
    26341 atatcctgtc agttttccat tttgcccgat cactcgatga caaggaataa cgattggcaa
    26401 tggattggtt cgatttgcct gcccaatcgc tcgcacagct ttcggactat caacagcaac
    26461 ggctacttct ttatacgttc ggatttctcc ataaggaatg gtcgacaaag ctttccagac
    26521 attttgctga aaaggcgtac ctctctttat agataaagga acatcaaatt cttttctttt
    26581 tcccgcaaaa tattcttcaa actgttcttg tgtgcgcgtt aaaattcgct gattcgctga
    26641 ttcgctttct gaaattggct gaaaagatac agcagtcaat ccggattcat ctgcatctaa
    26701 ccataaaaag ccgataggtg tgtgtatttt cattttgttt gctcctagtc aatagactgg
    26761 aacttcctcc tttttctcta tccaaaacta cttgccaact aataaaaaat gttcttaaaa
    26821 gtttgataaa ccgaggaaac atggtaaact aaatacgtta gtttacaaaa agaatacctg
    26881 ttcttttacg taaaatcaaa ttttaaaggt gttatttaag gtttattaaa ttttgaatgt
    26941 aaaccatata taaacatacg attgaaagga tagaaacgat gaaagtatca attgttgtcc
    27001 catgttttaa tgaagaaaaa actgttcctc ttttttttga tgaggttgaa aaattcaaag
    27061 gtgcccatga gttcgaatat ttgtttattg acgacggatc aaaagacggt acgttagcag
    27121 caatcaagaa gctagcctca gcttatcctt atgtccgctt tgtctcattt tccagaaatt
    27181 ttgggaaaga agcggctctg tacgctggat tgcaagcttc tacaggagat ttagtgaccg
    27241 tgatggatgt tgatctgcaa gatccgcccg aactgcttcc tgagatgatc aaccggatca
    27301 ctacttctga tatcgattgt gtcggcaccc ggcgttcgac tcgtgaaggc gagcctgcga
    27361 tacgcagttt ctttgcgaaa aaattctacc agttgatcaa taaaatcagc gatacagaaa
    27421 tggtcgatgg cgctcgtgat ttccgcttga tgactcgtca aatggtggat gccgtcctag
    27481 aattaacaga atacaaccga ttttcaaaag gattatttag ctgggtcgga tttaaaacag
    27541 aatatatttc ttatgaaaac cgcgaacgtg tggctggcga aacatcttgg tccttctgga
    27601 aattattcaa ctactcgatt gatggtatcg tgaacttctc ggatgcaccg ttaacgattg
    27661 cttcatttat cggcgctttg tcttgtgtcg gttctggtat tgcaattttg ttcattattt
    27721 tgcgggcatt gttgtttggt gatcctacat caggatggcc ttccatggtc tcgatcttcc
    27781 tgttcattgg tggaatccaa ttgttatgtc ttggaatcat cgggaaatat attgggaaaa
    27841 tcttcttaga aacgaaacag cgtcctgttt atatcataag agaaacagaa aaagtgaagc
    27901 caaatgaatt gaagcctcac caataatata cgtcaaatga agtaaacatt tttttaaatg
    27961 aaaaaggact gtgacaaaag tcttgtcaca gtcctttttt ccgaataaat ggtgttccag
    28021 aagtagcttc ttcggaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa
    28081 attccttctt atttcttgaa gctgaccact tctatcacaa cctctttcat atcttattac
    28141 ttcttcattg ctgcttttag tgctgctgct aatgggcttt cttctggttc ttctgcttgt
    28201 gcatattttt tcagcaagcg tcgttcttca tgtttggtca ttttcttctt ctgctctttt
    28261 ttatcaagca atttttctgt cgttccatcg tatttacaac ggaaataagc accgttttta
    28321 ccttcgatga tttccatttt ccggtgacat tgcgggcatc gatgattaga gacttttgga
    28381 tcttttcttc tgcggtagtt gcaatctgaa ttggtgcaga catagatttt gccgtcacgt
    28441 gtgtttttct cgcgcagatt ggccccacat tctgggcatt tcttttgggt aatcgagaaa
    28501 tcttgatatt cttgtttgct tgatttgatc tcattcacca agcgttttgt ttctttttcg
    28561 attccattca agaaggttcc tgcttgtttc tttcctgcag caatagcttc aaggtctttt
    28621 tcccagcttt ctgtcaaatc tggtgttact aaagagggat ttaccaatac taataattgc
    28681 ttacctttag gagatacttg cagtccttga ggggtacgtt ccatcagttc agatttgatt
    28741 agtttctcga taatctctgc acgcgtagct ggggttccca gactgtattt ttccatttga
    28801 ccaagcaagc ttgcttctga caatggttta ggcggtgcag atagttcttt tttgatggcg
    28861 aaatcgggat tgattctcat tcctttttcc catttgactg tttccgtctc agtttcagtt
    28921 tcgcctttcc atcctgcttg gacgacacga ttttgccgaa aaatgaatcg ttctttacca
    28981 aattcaaccg taacttttgt ttggctgact ttatggggtt gagcaaacag tcctaaaaaa
    29041 cgagagacaa tcatttgata gatttttcgc tcttcattac ttaatttttc ataacgtgct
    29101 ctgttttcag ttggcaaaag tgcatggtga tcggtcactt tactgtcttg gaatactttt
    29161 gtctgttgga cgatggctcc gtttttcaaa tactgcttga cctcaggagc aaagtcgctg
    29221 actgcttgca atcgttcttt catcgtactt ttcatgtcat ttgtcagata tttgctgtct
    29281 gtacgcggat aagtaacgat cttatgggtc tcatacaaac tttgtacaag acttaatgtt
    29341 ttctttgcag aatagccata gcgttgattg gcttctcttt ggatctctgt taaatcataa
    29401 ggcaatggcg cagattccgt ttttaatttt tcttggactt ctgtgacagt tcctttgctt
    29461 gtactgatca attgaacaag tttttgtgct tcttcttgtg tttttaaagc aaattgattt
    29521 ttttgaagca ttttggcttg ttcttttcca gcttgcaacg tgatcgtgaa atacgtttgt
    29581 ggacggaact gctcgatttt cttttcttgg tctctcacta atgctagtgt cggtgtctgc
    29641 acacggcctg ctgataaatt atccgcgtac ttcacagtta aggctctggt cacattcaat
    29701 cctaccagcc agtccgcttt tgcccgggca agagcagatt catacaatgc atcatattct
    29761 ttcgcaggtc ttagttgctt gaatccagtt ttgattgctt tatctgtttg agaagagatc
    29821 cataaccgct tcactggttt attgaaatgc acatactcta agatccagcg agcaacaagt
    29881 tccccttctc ttccggcatc tgtcgcaatg actgcttctg agacatcttt tcgctgagcc
    29941 agctgcttga ttgcttttaa ttgatgtcct gttttaggaa gtggtttgat tcctagtttt
    30001 ttagggatca ttggcaacgt gtccatttgc cagctctgcc attccttatt cagatcttcc
    30061 ggcattttca atcctaataa atgaccaagg gcccaagtaa cgatcacctt gggtccttca
    30121 taataatttt tgtgcttttg attagcacca agcaccttgc ttaggtcttt cgcaacgctt
    30181 ggtttttccg ctagaattag ttgtttcata aaattccttt ctgctttcta ctgtgatggg
    30241 ctgatgattt ttcaacaacg caagtccctt gtattgctgt aaatcatcgt cacactcttc
    30301 acaccagcgc tcatctccgt cattccaaaa ggcgctgaga aagttacttt ttgcattgat
    30361 atattcataa ttttgatgga tttcgttcca ttgtttttcg tacgcttgtc gtgcttcttc
    30421 gaaagactcg aatgtttctt cttcttcaat atcttcttcc cagtcttcaa aaaaccacca
    30481 aggctcattg tccccgaaca tggtaattac ttgatacata ctcgtgctcc ttctttcagc
    30541 ttttctagac tcatcttagt cacttttcaa gaaagttgca agaattttct cttataaaag
    30601 ggtcatttaa tgaacatctt aaaattgtta caaatgaatc tgattgttta aggcaaagta
    30661 gcgataaagt tattgatcat ccaaaaatag ctgtcttctg gatcgattgt tggttctttt
    30721 gtaaagtatc ctaaaaaacg taaggaaaga taaccgtgga tcatactacg aagttccctg
    30781 cttcgatgaa ctttttcttc tttcgttaat tgaaaagcct caaatatctg caaaatgatc
    30841 tgattcgttt catggattcc atctaataaa acagcttcat tggtatgcgg tacactgatc
    30901 agtaactcat acactgcttg atttgaaaaa gcaaaggatc gataagtttc tgcatagatc
    30961 tgcagtgctt cctgaccact ttttcccaca agagcccgtc gcagctggtc attaagttcc
    31021 tgcagaagtt tcgctgttag cgctgttttg acatcacgaa tatttttgaa atggttatac
    31081 agcgagggag ccttgatatc caagcgtttg gctaaatgct gaaaggttat ttgctgaaca
    31141 tccatctctt ctgctagttc tcgaaagcag tcgatgattt tctcttgaga cagatttctt
    31201 tttttcattt ccatgtttcc tctcataaaa taaatccttc actctttcag tttaccacga
    31261 aaaagaaaga gaaaaaagag actttcattg ataaactaca tattgtagtt tataataaag
    31321 agctgtagat gaaaattttc agaaagaagg agtctaattg gctgcaagaa taaaggaaaa
    31381 attatttagg aaaagagaaa cttgggaaca aaacttgttt gtcttatggt ttggaacatt
    31441 tatggctggt atcggattta gtttagtcat gccttttatg tcactttaca tagatacgtt
    31501 aggtaattat agtacgtcac aattgaactt ttggagcgga ttgacttttt catctacttt
    31561 cttggtgact acgcttatct ctccttggtg gggaaaatta gccgaccaaa aaggaagaaa
    31621 attgatgctg ttgcgtgctt cattaggaat ggcaatcgtc atttctttga tgggttgcgt
    31681 gactagtgtg tatcaattag tcgcattgcg tctgcttcaa gggatttttt ctggttatat
    31741 cagtaatgcc actgcccttg tcgcaactgg gacgccaaaa gaaaaaagtg gtcaagttct
    31801 tggaacctta gcaactggtt ctgttacagg aacattactt ggaccgcttc ttggcgggat
    31861 ctctgcttca gcgtttggct atcgccctac ctttttcatt acagggatca tcttattttt
    31921 cgtcttcttg ttgagtctgt ttttcgtcca tgaaaaattt gtcccagtga aaaaagaaaa
    31981 catggtttct gccaaacaga ttttcaaaga cttgaaatat ccacacgtcg tcatcggtat
    32041 gttcgtcaca acgatgatca tccaagcatc caacaattcc attagtccaa tcatcagtct
    32101 gtacattcgc caattgctgc atggacatgg gaatgttacg ttgatcagcg gaatcattgc
    32161 ttcgatacct gggattgcca ctttgatagc cgctccaaga tttggtcgct taggtgacaa
    32221 gattgggagt gaaagaatac ttgcgattgg tttgggtttt gcgattctgg tttatatccc
    32281 aatggcattc gtcacgaatg tttggcaatt agctgcctta cgttttttga ttggtatctc
    32341 tgatgcttgt ctgctccctg ctgtccaagc attgatcaca aaatattcgc ctcatcaagc
    32401 tgctggacgg atcttcagtt ataaccaatc tttccaagca actggtaacg tagtagggcc
    32461 tatgatcggc tccagtgttt ccagtgcatt tggttatcgt ggcgtctttc tttcgacttc
    32521 tcttcttgtt ttggtcaact tcttgcttgt acgacacaat acacaagaga tccatcagca
    32581 agaaactgca gaagaaaaag cttcaggagt ctccatagct tcagggcact ctccttctca
    32641 tagacaataa gcgtaaatga aaaacactgc ttgaacagtt gtcacagact gtccttgcag
    32701 tgtttttgtt taggcctgtt ttttcaagaa ctcagataag aggaataatc ccttttccaa
    32761 ggtttctttt ttacaacagt aacctagtcg ggcatgcttc ggtaaatcaa aggcacttcc
    32821 tggtactaat agtactcctg tttcttctaa taaattgata caaaatgtct ggtcatcttc
    32881 ttcgatggct aatttaatga aagaagtaga gacgtaattg ggagcgacaa gttcaacttt
    32941 cggttcattg gctacccatt gttttaaaat cacaaggttc tctgtaatga tctttttgtt
    33001 tcgcgccaat attttttctt tattcttcaa tgcatgtaca gcaagatcat ctgacaggac
    33061 tccgccgcaa atcatcgtgt agtctcgata ctttcgaaaa acttctgcca actcaggtcc
    33121 agtagctgtc cagccaatac gaatacctgg aatggagtag gttttggata gggaattggt
    33181 tgctatacct ttttcataga catctacgat cgacaagaat tcacctttat ccgttaaagg
    33241 cgcatatact tcatctatca atacatatgc atctactgta cgagcaattt ctgcgatttt
    33301 ttctaaagtt ttccgatcta gcaatgtgcc tgtaggattg tttgcactat tcaaacagat
    33361 gatttttgtt tcaggtttga ctaaggcttc tagctgttct aaatcaaact gccaatcttc
    33421 ttcttccttc aaatgaacaa agtcaacagc ggcacctaaa gatttgggga tgtcatataa
    33481 ttgctgataa gtaggaagca tcgaaacaac atggtctcct gcttccacta acgcgtatag
    33541 agctaaaaga ttagcaccag tcgctccatt cgtttgtaag atgttttccg gatcgacggt
    33601 ttgataaaga gaagcgactt cttgtttgaa aacatctgat ccttcgatcc agccatagtc
    33661 caatggttcg tttttgcgtt tttcgaaaaa tttacttacg ctagtcccat ctaagccaac
    33721 gagttcgtct aatgtcaatg cttcgatcga gctttgtgca atatcatata ccgctttctt
    33781 ttcccattta tttagccatt cttctacacc gaaatttgcg attttcatgt ttctttcccc
    33841 cttatatctt gatccagcct acagcaagca acccgattga aagcagtgcg gccacacaaa
    33901 tcagcccaat cattactttt tcttttcccg taatttgatg tttggtttca cgacaagcga
    33961 tccagtagaa gcaaaaacca ggaagatagg caatactaca taaaaggaca taagaaaatc
    34021 ctgctaaaat gatgcccatc aattcaaaaa ctacagcaat cccaccgatc actacttgtt
    34081 ttttgattcc ttcttttaaa gaaaactgga cttgatagat cccgacaagt agataacaaa
    34141 tcaaaattgc cgctgtacat aaagaatagg caaattgata ggcataatca gtaactaaga
    34201 aagtcaataa gaatagatta gtcaagattg cagtaacaac taacgaataa gttggggcat
    34261 tttttttatt cgtttttccc caacctttcg gcaacagacc gtctcgagcc atcaacatcg
    34321 ttgtttctgc tgggagcatc gtccaagaca accaagaacc aatgatcgaa ataatcaagc
    34381 cgatattgat cagcgctgct ccccaatttc ccacgattcc ttttaaaata tttgccatag
    34441 caggttgtga aatagttgct aactcttctt gagataagac gccgtatggc aagatcgaag
    34501 caaggatata gataaacaat aaacaaagca gaccaatgat cgatgcttgc tgggcttctg
    34561 aacgtttctt cgcgcggttg gctaaaacac ttgcgccttc gatccctaca aagacccaca
    34621 tcataaccat caaacaacct ttaatctgca accagatcgt tccttgctgc cctgttccat
    34681 tcactagatt atcagatacg ttaccccaaa aatcagcagt gaatatattc actttaaatg
    34741 cgataaatac aaatattaaa aataggaata atggcactaa tttacagatc gtcacgattg
    34801 tattgatgac gctcgctcct tcaactccat tgtttactaa taaagtcaga ccccaaataa
    34861 aaatacttgc aaataaaata gaaggaacat tttgtccacc tttgaatatc gggaaaaatt
    34921 ctcccatggc gctcatcagc atcgttgcaa aagcaacatt tcccagccac gcagacaacc
    34981 agtaacccca accactgatg aagccaccta atttaccaaa tgaagcttcc gcgtaaccga
    35041 aaataccacc atctagatct ggacgctttt ctcctaaatg attcagcgat aataccaaag
    35101 ctaaaatacc aatcccaacg attatccatg aaaggatcgc tggccctgga gctgcagaac
    35161 tagccagatc acttgtgatc ccaaacacac cgcttccgat tgacgaactg acaaccagag
    35221 caaccagtcc ccatttgcta atacccgtcg atttcttttc catttccatc accctcactt
    35281 ataaaattga acaaatcact tgcaaaagat tcccttctat tttgcaactg ctcttgctta
    35341 acaaaaagca actttattca tattattgga tattatgaac tttttacaca agtatttcaa
    35401 gtataaaatg aataaaaatt aattttattg caaaaaaaca gctatgctat ccaaaagaaa
    35461 aggtgtttct tttgttagcc atagctgttt tgattcattt aatatcgctt aaacgtattg
    35521 taaaactttt gtttcttgat atgcgtggtc gatcaaacca ccacccagac attccattcc
    35581 atcataaaat acaactgctt gtccaggagt gattgcacgt acaggttcgt cgaatacgac
    35641 ttctgcacga ttatcatcca ataagcgaac tgtcacagga acgtcttgct gacgataacg
    35701 gaatttcgct gtacacttga attcttttgg cattggttca ttcgtcgtaa agtggatttc
    35761 acttgcatcc aagcttgtgg catacaatgc aggatgatgg aacccttgcc ccacatataa
    35821 tgtgtttgtc gctagatctt ttcctacaac aaaccagggt tcttgcgaat ctcctccgcc
    35881 accgatccct agtccttgac gttgaccaat cgtataatac atcaagccag catgttgtcc
    35941 tttgacttca ccatcaagag tgaccatatt tccttttttt gctggcagat agttgctcaa
    36001 aaattgtttg aagttctttt caccaataaa gcagattccc gttgaatctt ttttcttagc
    36061 agtcgcaagg cctgcgcgtt cagcaattgc ccgtacttct gatttttcca tgccgcctag
    36121 agggaacatc gtttttgaaa gctgttcttg tgataattgg ctaaggaagt aagtttgatc
    36181 tttattattg tctactccac gaagcatgtg gaccacgccg ttttcatcgc gagtgacttg
    36241 cgcataatgt ccagtagcta cataatcggc gccaagatcc attgcataat ccaagaatgc
    36301 tttgaatttg atttctttgt tgcacatcac gtctgggtta ggtgtacgtc ctgcgcggta
    36361 ttcggctagg aaatattcaa atacacgatc ccaatattct ttttcaaaat tcacagagta
    36421 ataaggaatc ccgatttgag cagctacttt cgctacatcc ttataatctt ccgtggccgt
    36481 gcatacaccg ttttcgtccg tgtcatccca atttttcatg aatattccga tcacgtcgta
    36541 tccttgttcc tttaacaaaa gtgctgttac agatgagtcg acaccgccgc tcatccctac
    36601 gacaacgcgt gttttgctgt tgtctgtcat gatatcacca tcatttcaat tagattctga
    36661 aacatctacg agttgaaggt cgcaactttt cagtatttta cattatacgt catatttctg
    36721 aaaatttcat cccttaatta tcaagtcgta aaaatactgt tctactggac ctattcagta
    36781 cacaaacatg atagtcaacc aaaaaatcac tcatcttttg agcattcgat ataaaaaatt
    36841 ggcatactca taaaaagggc cagcctttcg gcaataagga ataattttat aaattgtgaa
    36901 aaactatttt tcaaaattgc ttcttatcgc ttaaagctgc acgtctcatt tacaaccttt
    36961 tctttttaga tgttatgagt tgctcagata agtgattttg tttactctaa gaaaaaaggg
    37021 aggcaaaaga tacatgaatt actttatcag tgatatgcac tttttccatg aacgcttatt
    37081 aggaaaaaac gactttgctc cgcggctctt tgcaaatgtg gatgaaatga accaacagtt
    37141 gatcactagc tggaatgcag taataaaaga aaccgaccat gtttatcatc taggcgacct
    37201 tgctatgcat ccgcaatatg aaaaagcaaa tgcagaaatt ttggaactgg tcactcaact
    37261 gcaaggaaca atccacttga tcaaagggaa ccatgacagt cgcgcttttt tccgttattt
    37321 agaaaaaaat gatcctaggc ttactgacgg aagtcccaaa ttttattttt atgatgtagg
    37381 tgccatcgtt aaattcgacc atcaacaata ttacttgact cattatccga tgttgctcgg
    37441 gcctaacgac actatccgca atcttcatgg acatatccat cattacagcg tacctatcga
    37501 gaacgatgtc aatgtaggcg tagacgcacc ggaaagagaa ctcttaaagg aaaaactacc
    37561 atttggcact ccgttatctg agcaacaaat caaagaaatc tatgaagcaa agaggaagta
    37621 cgataggtcg tgaaaaaact gccctatatc aagtattaaa caaataaaga taattactca
    37681 tatttttcat ttgagtaggc ttattgccac aggaacatta ggatcagaaa tggagttgta
    37741 acaagacttc tgtcacaact ccattccata ctattcgtct ttttgctcat ggtgttcttt
    37801 taccagtttt ttctgtaatt ctttatcata atttcgggca gctgacgtta aaaataaaac
    37861 gattccgcat aataaaatcc cgattccgga aaacaagaac attttttcta cccctaaacg
    37921 atcagctaat ggtcctgcaa ataataatcc aattggacct gtcaggttca tcaaagagtt
    37981 gaagttccct aagacacgac ctaaaacatt cgattcataa ctctgctgga tcattgccat
    38041 cagcagtgta ttgaaatatg gcgttgcaca acctgctcct gcattcaaga taagaaagta
    38101 caagaaccct tgactatcgt ttggaaccaa tccgcttgca ccgatcgtca ggcccataat
    38161 gatgtaggct ataataatag gtttcattcg gtctttccat gttcctataa aactgatcag
    38221 tgcaccgcct aagagcattc cgactgcgta gactacttcc actgctcccg cctgtccaac
    38281 ggaaccacca aagtaatcta gcgtcatcaa tggatacaga ctgattgctg gcatgaacaa
    38341 cagcatcaca aatgcaccat tgatcgttat gtaccataac cctttattat ccatcaattg
    38401 ctgcagccca aattttgcat ctttaaaaat agtaagcgga acttccaata tttcaggaga
    38461 aacttttggg atcttcacaa aaatcaataa gccgacacca aaaactgccc ccaatacatc
    38521 taataaaata aggtaattga caggaacgac tgaaaacaaa gccgcaccta gagctggcgc
    38581 aatgatgaaa ttcgctgatt ggaccattcc taactgtcca ttcgtccttg ttatgtgaga
    38641 agatggaacg attgtcggca taatcgactg gatcgttggc atttggaatg tttgtgcgac
    38701 cgaacgcata aacaaagaca caaaaactag ccaaatcggg aaggattctg aaatcgttcc
    38761 gactactgct aaaataagcg cgaaaatcgc tacaataata tccgtgacaa tcaaaagagc
    38821 ttttttatcc cagcgatcca ctagcggtcc tacaaatggg cttagtaaaa ccatgggaat
    38881 cattcctagt aaagtagcaa aactcaaaat tgtcgccgaa ccggtttctc ttgtcaaata
    38941 ccagatgatc gcatattgga cgaccatact agtgattcct gacaaaaact gtcccgataa
    39001 aaagaacaaa taatttctct tccaattcgt taaaacgacc gactgttcat tttccataaa
    39061 catccctctt ttctttttca ttttgtatag cgtttgcaca acaatcatta tacgggattc
    39121 ttttttcaaa aaataccttt ttccctaaat aacaatcgtt tcaatttaaa tttctttact
    39181 tagaaacaat aaatcagcaa aatattaaaa ataacaacat gtgttaaatt taaaaataaa
    39241 aaagacaact aatttgcgtc gtccttacaa agttagtcat ctaacaatag gccgtttcaa
    39301 aactagttgt ctttttatat ccacagatca ttcgactttt tcgaataacc cacggctaat
    39361 catcccatcc atcagaaaat aaaatttatc ttggctcatt ttctgttttg gatctttgaa
    39421 aatattgact gctttcaatc catttgctgt agtgatcgac attttcaatg tttgaatatc
    39481 ttttgataca gtgattttta atttgaatcc ttctttgctt tgcggcgtgg catctaatgg
    39541 tcggatcaat tgataattgc ctccgtttgt ttctgtaaat ccattatcgc gcaatgtata
    39601 tctttttgca tcaggatgca aacgatagct aattggtgcg cccagtaccg ttgctgttgt
    39661 ttcaaatgcc atgattttca cctcgataaa attagtttct ctttattata aaagtttatc
    39721 ctgattttta ctaggttttt cagttaagtt ccctaaaaaa tgaaggcgtt ctctcagaca
    39781 tctgttcata taagaatgac ttcactagtt attttttgac agcacgaact aattgatcaa
    39841 ggaaatactg gatgtcgtct tcggtatttc ccaaaccaaa gctgatacgg atggattctt
    39901 ttagtatcgg tgtatcttct ccatacattg cttccaatac gtgtgaaggc tcgatatttc
    39961 ctgctgtaca agctgaacca gtagaaatag cgaccccatt aagatcaagc ttcatcagta
    40021 gcaaatcatt tgcttcgcca attaagcgaa gattcaacac atgagggagt ttattcgtta
    40081 aatcaccatt tacttgataa gggatttcgt ttgcttctaa tgtatcaaga atcaattgct
    40141 ggaatccttt atatttgttg ttacgtgctt cacgttcttc aggtgacagc agttcaactg
    40201 cttttgccat accccagatc cctgctaaat tttctgtacc tgcacgccgc ttttcttcct
    40261 gctccccgcc taaaagcaat gcagggatat ttactccgtc tcgtttatat aagaaaccga
    40321 cgccttttgg cccattgatt ttatgagcag agatacttaa taaatcgatt cctaattctt
    40381 ctggatggat cgtttggttt ccgtacgctt gtaccgcatc tgtatggaaa taagctggat
    40441 gatccgctaa aagttcaccg atttctttga ttggcatcaa atttccgatt tcattgttcc
    40501 cgtacatcac agagaccaag atcgtatctt ctcgtaacgc ttttttgacc tcttcgatcg
    40561 acagattccc tttttcatct acaggcagat atgtaacgtc aaaccccttt gtctctaaat
    40621 atttcatggt attaagaaca gcaggatgtt cgatagctgt agtgattaga tgtctgcctt
    40681 ctttttgacg cgagaacgct gtttctaaga tagctgtatt atccccttct gttcccccac
    40741 tgttaaagat gatctcgtga ggttttgctt gcagactgtc tgcaatgatt tgtcttgctg
    40801 tttccaattt ttcatgcgcg tggcgaccaa atccatgtat gcttgaagga tttccaaacg
    40861 tgtcttgcat gattgccgtc atcgcttcaa ttacttgtgg atgcatgggg gtagttgccg
    40921 catgatccag ataaatagtt ttcacttcat tcatcccttc caaattattg ctaggcacat
    40981 tctagcataa aatagcgaac aaaccaaaga agagctcatt tttgtctaga ggacgtttca
    41041 tgggcataga aaaaaggagc tgtgacaaaa gtcttgtcgc agctccttga agctgctcac
    41101 ttctgtcaca acttctt
//
LOCUS       DARBFO010000014        40414 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000014 DARBFO010000000
VERSION     DARBFO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.014"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="SCK51_000902"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="SCK51_000902"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEG1251546.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="SCK51_000903"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="SCK51_000903"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEG1251547.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="SCK51_000904"
     CDS             complement(2303..2965)
                     /locus_tag="SCK51_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1251548.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="SCK51_000905"
     CDS             3149..4795
                     /locus_tag="SCK51_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEG1251549.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="SCK51_000906"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="SCK51_000906"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG1251550.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..7529)
                     /locus_tag="SCK51_000907"
                     /pseudo
     CDS             complement(5976..7529)
                     /locus_tag="SCK51_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7677..8818
                     /locus_tag="SCK51_000908"
                     /pseudo
     CDS             7677..8818
                     /locus_tag="SCK51_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8944..10023)
                     /locus_tag="SCK51_000909"
     CDS             complement(8944..10023)
                     /locus_tag="SCK51_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1251551.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10126..10566)
                     /locus_tag="SCK51_000910"
     CDS             complement(10126..10566)
                     /locus_tag="SCK51_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1251552.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10623..11033)
                     /locus_tag="SCK51_000911"
     CDS             complement(10623..11033)
                     /locus_tag="SCK51_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG1251553.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11213..11647)
                     /locus_tag="SCK51_000912"
     CDS             complement(11213..11647)
                     /locus_tag="SCK51_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1251554.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11747..12595)
                     /locus_tag="SCK51_000913"
     CDS             complement(11747..12595)
                     /locus_tag="SCK51_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1251555.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            12984..14750
                     /locus_tag="SCK51_000914"
     CDS             12984..14750
                     /locus_tag="SCK51_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEG1251556.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            14852..16111
                     /locus_tag="SCK51_000915"
     CDS             14852..16111
                     /locus_tag="SCK51_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1251557.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            16258..17559
                     /locus_tag="SCK51_000916"
     CDS             16258..17559
                     /locus_tag="SCK51_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1251558.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            17562..18413
                     /locus_tag="SCK51_000917"
     CDS             17562..18413
                     /locus_tag="SCK51_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1251559.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            18426..19241
                     /locus_tag="SCK51_000918"
     CDS             18426..19241
                     /locus_tag="SCK51_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEG1251560.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            19392..20558
                     /locus_tag="SCK51_000919"
     CDS             19392..20558
                     /locus_tag="SCK51_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1251561.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            20788..21435
                     /locus_tag="SCK51_000920"
     CDS             20788..21435
                     /locus_tag="SCK51_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEG1251562.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            21519..22463
                     /locus_tag="SCK51_000921"
     CDS             21519..22463
                     /locus_tag="SCK51_000921"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEG1251563.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            22598..24049
                     /locus_tag="SCK51_000922"
     CDS             22598..24049
                     /locus_tag="SCK51_000922"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEG1251564.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            24070..24906
                     /locus_tag="SCK51_000923"
     CDS             24070..24906
                     /locus_tag="SCK51_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEG1251565.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            24906..25565
                     /locus_tag="SCK51_000924"
     CDS             24906..25565
                     /locus_tag="SCK51_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1251566.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            25691..26380
                     /locus_tag="SCK51_000925"
     CDS             25691..26380
                     /locus_tag="SCK51_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251567.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            26386..27342
                     /locus_tag="SCK51_000926"
     CDS             26386..27342
                     /locus_tag="SCK51_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEG1251568.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            27386..27898
                     /locus_tag="SCK51_000927"
     CDS             27386..27898
                     /locus_tag="SCK51_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1251569.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(28154..28702)
                     /locus_tag="SCK51_000928"
     CDS             complement(28154..28702)
                     /locus_tag="SCK51_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1251570.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(28791..29972)
                     /locus_tag="SCK51_000929"
     CDS             complement(28791..29972)
                     /locus_tag="SCK51_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEG1251571.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(30208..31716)
                     /locus_tag="SCK51_000930"
     CDS             complement(30208..31716)
                     /locus_tag="SCK51_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEG1251572.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            31875..32066
                     /locus_tag="SCK51_000931"
     CDS             31875..32066
                     /locus_tag="SCK51_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251573.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            32269..33186
                     /locus_tag="SCK51_000932"
     CDS             32269..33186
                     /locus_tag="SCK51_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEG1251574.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            33179..34252
                     /locus_tag="SCK51_000933"
     CDS             33179..34252
                     /locus_tag="SCK51_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251575.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            34400..34918
                     /locus_tag="SCK51_000934"
     CDS             34400..34918
                     /locus_tag="SCK51_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251576.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            34899..35291
                     /locus_tag="SCK51_000935"
     CDS             34899..35291
                     /locus_tag="SCK51_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEG1251577.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            35367..36302
                     /locus_tag="SCK51_000936"
     CDS             35367..36302
                     /locus_tag="SCK51_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEG1251578.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            36435..37214
                     /locus_tag="SCK51_000937"
     CDS             36435..37214
                     /locus_tag="SCK51_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEG1251579.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            37207..37524
                     /locus_tag="SCK51_000938"
     CDS             37207..37524
                     /locus_tag="SCK51_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEG1251580.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            37768..39378
                     /locus_tag="SCK51_000939"
     CDS             37768..39378
                     /locus_tag="SCK51_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1251581.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            39535..40383
                     /locus_tag="SCK51_000940"
     CDS             39535..40383
                     /locus_tag="SCK51_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1251582.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        13231 a         7260 c         7799 g        12124 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
     6841 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
     6901 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
     6961 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
     7021 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
     7081 atagacaaac cgtactatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
     7141 gaggcactat agtttgtttg ctgactgaaa ctgataatcg aatttgtctg acagctgaac
     7201 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
     7261 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
     7321 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
     7381 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
     7441 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagata
     7501 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
     7561 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
     7621 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
     7681 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
     7741 agacagtcca cacagaggag acagaaacag ccaaatggac agaaaccaag tgggccaacg
     7801 tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa
     7861 gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcgggagac ttagcaccta
     7921 tactacaatc agctgttaca tttgagaatg ttgatcccac tttgtcgatt ggctatgacg
     7981 agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc
     8041 aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc
     8101 atcatttaac gccgtatagt cagcggcaga caattgagca agattcagcc atcgactcag
     8161 gaggagatct tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac
     8221 cgcctacaga aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg
     8281 atacagaaac tcaaccagag ccagacaata aatcaaaacc tgaaataacc cctggagaaa
     8341 aaccagatgt aactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa
     8401 atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagat ccagaagaaa
     8461 aaccagatgt gactccagaa ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa
     8521 taaacaaaaa aacaattcaa gaggatggaa aaaaagagag taaaaaatca aatctagcaa
     8581 ttctaaaaat aaatgaagag cagctgaata aaaaatcacg aatatttgat agcgcccaat
     8641 cagctgaaac cttaaaaagt agcaaggata caacctttgc tgtcctgaaa cgaaaaataa
     8701 acagctacca aaatccggag agtcacaaaa caaagttata ctgtggagcg gaatcattct
     8761 attaagtata gcaacaatgc tttctgcaaa aagatttaaa caaaacagat ctctctaaca
     8821 tttgaataat gtctgtgaac gcatttttca aataaaaaga aatatccaga aaatgacccc
     8881 aaaataccag attgaaatct gatatttggg gtcgttcata ctttatcttt cttcatattt
     8941 attttaaggg atatctgttt cttttggaag atggttcggg atctttttca ctttcaatac
     9001 atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg
     9061 ttcgctaaca agtaagacca caaatgtata aaaaatcggc agttctgttt tgctggacat
     9121 caacttcgga ttcagcacat aagactcaat tagatggaca atcaaaatcg taatcaggat
     9181 ataaacaaca tctcggatgc ctccttggga ataagctatg aaagatagag gaatacaaga
     9241 aatgataaca cctgctactg ggatcagact taaaatgaaa atcatgatcg ctaaactcag
     9301 cagctgatga aagccaaata cagccaaggc gattgtagtg agcaacgtat ttaaaagagc
     9361 gacaacaaat tgagcttcta ataccaaacc aaatgtattg acaaacttat cggcaaaata
     9421 gtaaatatct tgaaagaacc aagaaaattc actttttaaa aacaatcttg agaatacaat
     9481 tgtttttttc ttttcaatca taaaaaagaa acttaagatg aatgacatag caaacgccac
     9541 tgccattttt cctatatcct gtaaatagcc aagcgcaatc gaagcgccat tttgaagctg
     9601 ttcaaaaaaa tcgtttcgct caatataagt atggatatat tgtaaaacag gatctgcatc
     9661 ttttggctga tgctcgtaaa aattaataac ggactggatc atgtcaaatg tttgatgaat
     9721 aagcatcggg atatactttg tgaatgccag atagataagg taaacgatca gtgtataaat
     9781 catgatagct aaaacacctg tagggattcg gacaaatcgc tgaatgattt gaacaagatg
     9841 aatcatcaaa taagtaaaga taaatgtgag caaaatggtt gtaatcatac ttctaactaa
     9901 gaaaagaaca aaaacgacaa ataataaaac agtgagacgg cgtatttttt cattcgccaa
     9961 aaaacgctga taaacggtca aatcgacaac ctccaaataa caaaaaataa acacgttatt
    10021 cattatatga tggaatatga aaataagaaa gagcaaaaag aaaaaccgct aaatgacgtt
    10081 atcttatgag ttggttttcc tcataaaatc gttcatctag cagtttcagc cctttttctg
    10141 gtcttgatga aaattatcta gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc
    10201 ctgttcaagt ggaagataag acttgatctt ttgtggaata ccttctgctt gtttttgcaa
    10261 cgcagcacct tgagcagtca gcgtaacatt tacgattctt tcatcacccg tttcacgctg
    10321 acgattaata taattttttt ctgctaactt ttttagtaag ggagtcagcg taccagaatc
    10381 cagacacaaa tcatttccta gctctttcac tgaagtcttt ttcttttccc ataaaactaa
    10441 catcacaaga tactgggtat aggtaagatt cagcggcttg aggaggggag tgtagtgtct
    10501 aatgatttct ttagaggcgg catataaagg aaaacatact tgattaacta aatatagatc
    10561 ttccattttt ccacctccgc tgttttacta tagtgtatca tagcggattt acacaaaaaa
    10621 gtctattcac taactgcttt cagagtaact tctatatttc cagctgtagc ttttgaatac
    10681 ggacacactt tatgcgctgc ctctaataat tcttgcgttt cttccagaga aagaccttcg
    10741 atagatcctt caatctctac acctaagaca aagctcgttt cactttctga ataaagggaa
    10801 acttgtgcac tgacagtaga agccccagag atattttttt gctgtttgat caattctaat
    10861 gcactgttaa aacatgcact gaaaccagct gcaaacaatt gttcagggtt ggttgcattt
    10921 tcgacgcgtt taccaggagc tgcaatattc aattcgaatg tacgatcggg attatgcact
    10981 tctccagagc gtccgcctgt gttgatcatt ttcgtagaat acattttttt cataaaatta
    11041 ccacctttaa ttatatttta tacaattgaa ttttacacaa tataattgtg gaaagcaaag
    11101 aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc tttctcgaat
    11161 agcaaacata tacaaaaaat atcagagaca aaaaataatc tcatcataaa cattacatat
    11221 atttctcaaa aagttgtttt ctttcttcaa aaattacttc acgatggacc tgatgaagca
    11281 tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag tccatcattt
    11341 ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt gtgtcctcga
    11401 tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta ggagcagtca
    11461 aaccaagaga cgtgttttca tccagcatat ggaatctcgt accatacaag gtaaaaatga
    11521 cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca tcaaagattg
    11581 tttgatacag ttctagtgct ttcaaactat catttaatac aaaatcgatt tccgttcctt
    11641 tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt ttattatacc
    11701 agctaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat tcgattggat
    11761 gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca aaggcacttg
    11821 gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc acaacgatca
    11881 ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg gtaaggccac
    11941 gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca ctcatgacca
    12001 taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt gaagtgattg
    12061 caatagataa aatagcataa agaaactctt ctattccaaa aataaacaga ttaagagaaa
    12121 caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa tattttttaa
    12181 aaatgagggg agggatcgtc gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa
    12241 ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca
    12301 ttgtttctgg aactattgca atgattaaag gaaaaaccaa gcttccgaaa agtactttaa
    12361 aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta
    12421 aaataactat agctcggtca aagcctaaag cagcttccag taatattcct agaccgctga
    12481 cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga
    12541 agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatt
    12601 cacttcctta attcattaat aatactctta tcttattata acttactttt caccttaagt
    12661 taactttaat taataaaaaa attaggctag tctaaattat tatgtttcat tcttctagaa
    12721 tatacttcta cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat
    12781 cgcttaagat aaattcagga cactaaatag tacaggaagt agactaaaac ttttaaaagt
    12841 tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac
    12901 cgataacatt tttgaaagcg ttgtcttttg cttctctatc acttatagtg atgacgtcat
    12961 attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg
    13021 aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg
    13081 atatcaaaaa agtatatgtg ctatgtggag atccatatac cataacaact gaaaaatggt
    13141 atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa
    13201 ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt ggggaagata
    13261 acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg
    13321 aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag
    13381 aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca
    13441 aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact
    13501 ttttcggggg agatttgcaa ggtgtgatgg atcatttgga ttacttgacg gaccttggca
    13561 tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga
    13621 ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg
    13681 atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg
    13741 tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact
    13801 ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg
    13861 ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag
    13921 caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg
    13981 atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat
    14041 tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt
    14101 cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcatttacag
    14161 attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa
    14221 tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag
    14281 attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt
    14341 ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa
    14401 tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg
    14461 aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt
    14521 ctaagattat ttctgaaggt tctactcagt ggctaatagt aaatgatcgt gaagataagc
    14581 tctatttcac acggaaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag
    14641 aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag
    14701 aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac
    14761 cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa
    14821 cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg
    14881 tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc
    14941 ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata
    15001 taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa
    15061 gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg
    15121 aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg
    15181 acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca
    15241 agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta
    15301 ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc
    15361 caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa
    15421 atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg
    15481 tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat
    15541 ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag
    15601 tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg
    15661 tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc
    15721 tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag
    15781 caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga
    15841 aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga
    15901 aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc
    15961 tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat
    16021 gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac
    16081 aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa
    16141 aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct
    16201 tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg
    16261 cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca
    16321 gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc
    16381 gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact
    16441 ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa
    16501 ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga
    16561 gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat
    16621 aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt
    16681 tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc
    16741 aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc
    16801 ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg
    16861 actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac
    16921 cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc
    16981 ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc
    17041 aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct
    17101 tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga
    17161 tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat
    17221 gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct
    17281 gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat
    17341 ttctccatga tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg aggagctggt
    17401 tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat
    17461 tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg
    17521 gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga
    17581 gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt
    17641 cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata
    17701 ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat
    17761 tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa
    17821 tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta
    17881 tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag
    17941 cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag tactggttct
    18001 tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact
    18061 ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca
    18121 ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat
    18181 gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg
    18241 aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag
    18301 tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct
    18361 ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga
    18421 gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt
    18481 ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta tttttagttt
    18541 ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg attccattag
    18601 aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac
    18661 ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa
    18721 ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt
    18781 ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga
    18841 tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat
    18901 ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa
    18961 gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct
    19021 tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt
    19081 tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact
    19141 ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg
    19201 tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg
    19261 cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat
    19321 taaacttctt agttgattct tttctttttt agtattaacg taatagggta gtttttttaa
    19381 ggaggaaaat catgttagag aaaactttat acaatcaatt attttcacgt tctttctcgc
    19441 tccctgtcga agtaaattat tgggacggta caacgaaaca atacggagat acggacactc
    19501 ctccccaaat caaaatcaat atccatgaag aaatcccaat gaaagagatt acaagtaacg
    19561 catctttggc actaggagaa gcctatatgg atcatcgaat cgagatcgag ggaagtatcc
    19621 aagcattgat caacgatgct tatcagcaag caaacagctt tttacgagga aaagattatc
    19681 taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg
    19741 cgcattatga cttaggtaac gacttttata aactttggct agatccaact ttgacttatt
    19801 cttgtgctta tttcaataca cctgaagata cgttggaaca agcacaaatc aataaagtcc
    19861 atcatatctt agacaaactc tttatcaaag aaggcgaaac gttgcttgat atcggttgtg
    19921 gctggggaac attgatgttc actgctacca aagagtataa tgtcaaggca actgggatca
    19981 cactaagtga agaacaatac gatttgatta ccaaaagaat aaaagaagaa catttagaag
    20041 ataaatgccg tgtattatta atggattacc gcgaactaaa aggcgaaacg tttgatcata
    20101 tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag
    20161 tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat ccatggaatc agccgacaac
    20221 aaggcggtgc gacaaatgct tggatcaata aatatatttt ccctggcggt tatgtccctg
    20281 gtgttactga attagttgat aatattacaa aaaacgattt gcaactgatt gatttggaaa
    20341 gcttacgtcg tgactaccaa ttgacattag aacattggac aaagaatttt catcaagtcc
    20401 aagatcaagt cacgaaagaa atgggtgatt ccttctatcg aatgtgggat ctatatttac
    20461 aagcttgtgc agcttcattt gaatcaagta atatcgatgt gattcaatat ttgcttgttc
    20521 aacctggaaa caatgacatc ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa
    20581 agactattta aaaagaagct aagttttatg atgctccaga agtgaacttt tttgtctaac
    20641 ttctaggatt gtctctaaag cttagtttct ttttctttat attttcataa taaaagtttg
    20701 gttctttaat ttttaaacat gacagaaaat tttttcttcg ttatacttgt aatagaatac
    20761 tattttataa caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt
    20821 gcagctttgt ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg
    20881 tcttccagta gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca
    20941 tcttcgtcta gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg
    21001 tctgcagctc gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt
    21061 ttggaattag atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa
    21121 aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa
    21181 actctagatc gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat
    21241 aaattgatca cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg
    21301 gcaacagatt gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa
    21361 gacggcaatc aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact
    21421 gaacaagatg attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat
    21481 acactagaca aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg
    21541 ccaatcatat ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc
    21601 aggaattaga tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga
    21661 tcaatgaacg aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca
    21721 attctatggg acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg
    21781 aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc
    21841 aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat
    21901 taaatttatc ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca
    21961 cttatgaaac actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc
    22021 ctccttattt tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat
    22081 tgacctatgt caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag
    22141 aagcagtagt catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc
    22201 cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca
    22261 ttggtacagg tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa
    22321 gcatgttaca gattggtacc gagcttcata aagaagggcc ggaaattttt tctagaatca
    22381 taaaagaatt aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag
    22441 gcaaattacg caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt
    22501 ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa
    22561 cttgtgggaa aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc
    22621 aaaagagctt aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa
    22681 aataaagaag caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa
    22741 ttcccacgag ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc
    22801 tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt
    22861 tgaaacacac cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct
    22921 agttcctttt ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt
    22981 catctatggc ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg
    23041 aggagctgct gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg
    23101 tggttctatt agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg
    23161 gacgatgcca gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg
    23221 cttgacagtt tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc
    23281 cggtgcgcca tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt
    23341 gaagatagct ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact
    23401 tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc
    23461 ttacccacta gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac
    23521 agcagcaatg atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact
    23581 tgaacccatt tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat
    23641 cgattctctg catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata
    23701 cgatctattc ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca
    23761 aagtgatgcg ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt
    23821 agacgttgtt tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc
    23881 taatctgact ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc
    23941 tctaggaatc caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta
    24001 tctatttgaa gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg
    24061 agaagaagaa tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta
    24121 agcgcttgcg gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa
    24181 aagaaagaat tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat
    24241 caaaatgaat tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat
    24301 gtcaaaatct cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga
    24361 gaatcagatt ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc
    24421 ctttcagaga ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca
    24481 ggaatccact cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc
    24541 tatatgcgtt tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat
    24601 gatcagtatt taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat
    24661 ctgcagaaaa tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt
    24721 tatttcaatg aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag
    24781 aaaatcgatg gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa
    24841 aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt
    24901 gagtgatgta ctttccccta attgattttt ccctcatgtg gacttcactt cctttcgtac
    24961 tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatttgg
    25021 tagggttgct tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc
    25081 gtttttattt atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt
    25141 tcgggttacc ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca
    25201 gcagtgcatt tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt
    25261 gggacgctgg tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc
    25321 cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag
    25381 gtacttcgtt agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag
    25441 gcggtcggac attcgattat ttttctctct atattttagt tgcactgatt tattgggtca
    25501 tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa acgttctatc catcagtcaa
    25561 tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta
    25621 tattctgcct agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga
    25681 ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg
    25741 catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt
    25801 agatacgctt agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct
    25861 gaaagtgaca aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa
    25921 ttattttgtt ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat
    25981 aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt
    26041 tctgattgcg aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa
    26101 ccgcttcatg gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt
    26161 aaaatatttt ccaaaagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga
    26221 tccattttct aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat
    26281 caatgagttg caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca
    26341 acagcagatc cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat
    26401 ttaagaggaa aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc
    26461 tccaataaaa tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg
    26521 atcgaattat ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc ccaagtagga
    26581 caattcaaat tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc
    26641 gtccctgcac agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg
    26701 tttcttgttt tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca
    26761 ggagttgatt cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa
    26821 aacaaaattg tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat
    26881 aaaacagatg acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga
    26941 ttttctgatt tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg
    27001 cttgctatcc atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc
    27061 tctgacagta acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa
    27121 atgaaaaaat ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa
    27181 ttattggctc tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca
    27241 cttatgcatc atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa
    27301 tattattcaa aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa
    27361 aaaggaatag aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga
    27421 agaagtggta gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca
    27481 atggcaaaac gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga
    27541 aagctatgta ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaggcgt
    27601 ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc
    27661 gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt
    27721 agaacatttg ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta
    27781 tccagctaat gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat
    27841 gattgaattt ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact
    27901 ataaaatcat ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat
    27961 agccaagagg ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat
    28021 gcttctcaaa tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc
    28081 ccttcatcga gaggtcgtga catagctttt gtcacgatct ctcggcttat cctacgagta
    28141 ttattcgata catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg
    28201 ctccatcgtt tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta
    28261 aatcttctac ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt
    28321 tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc
    28381 cgaaatactc atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta
    28441 ctgtcagttt ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt
    28501 cccactctgc aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat
    28561 aataatcctc ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct
    28621 tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga
    28681 taaatacata gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc
    28741 tttggaaaat atttccaaag cttttaaaag taactttgct tttttgtatt ttataccagc
    28801 aagccagtca caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat
    28861 ccgtgttttg cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg
    28921 atcccgatag aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta
    28981 cgctcagtaa aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta
    29041 accagtttag tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg
    29101 atgaaggcaa gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc
    29161 ccgtggaaga tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca
    29221 accgtgatcg ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc
    29281 tgttctttgt tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt
    29341 acaattgaag ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat
    29401 ttagaatcga tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc
    29461 gatacactca tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa
    29521 ataaaaactt ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg
    29581 cccatcccgt tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc
    29641 ttgatatagc gtagaatacc gataatagca gaaataaata caattggcat caatacggaa
    29701 agaaagaaag gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc
    29761 ccagcaaaag caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca
    29821 aaagttgtac gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt
    29881 ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa aagcaagccc taatgtgaca
    29941 aataaaccaa taatacctaa taaataactc aaataatcac tcctattcca aattccgttt
    30001 tattttacct gttttgcaaa taaaatacta atctttagtg aaattttcat tactttttta
    30061 gagtgaaacg cattcatttt ccattagaaa ataaagcgaa caaaataaaa ggtttaaaaa
    30121 gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat cgattaagct aatagtttcg
    30181 ttttcaacat ttataagtaa actttttcta ttggtaatat agattttcgg aagggtagac
    30241 aggtgcgcag gtaatatcga ttcctagttt tttcaatgtt tgttcatcat ttttattgac
    30301 catcacagta gagtgggctt gtgctccctc aagttcagct agtttatcat aagcaacttg
    30361 agcgattgga ttcgttactg cactgattga taaagcgatc aagacttcat tgatgcttag
    30421 tgcatccaaa gaggaatgaa gtgtctttga tttcaatcct tgtattgttt caagaactaa
    30481 tggtgacaat aaaggaatgt catctgcgat atgggctaaa tgttttactg catttaagat
    30541 aactgccgct gacgcatcca tcaatgaact tgtacgcccg gtaatgatga cttcatctgg
    30601 taattcgatt gcaatcacag cagaaggatc ttttgtagga ctttgcgctt gaatcttatc
    30661 tgcatagtca tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac cgacttcttc
    30721 cataatcagt ttgattcgat taactgcttc ttcgtcgatc aatccttttt tgaagtcgca
    30781 ttctgtttgg aaataacgac ggatgatttc ttgtttagag gcttcttgga caacggtatc
    30841 atccacgatc ccaaatccta cacgattcac acccatgtca gtaggagatt gataaacaga
    30901 ttctttacca gtaatttttt caataatacg tttgattaca ggaaatgtct caacatcccg
    30961 attatagttc acagctactt tattgtaggc atcaaagtgg aaggaatcga tcatattgac
    31021 atcttttaga tcgactgtgg ctgcttcata agcaatgttc agcggatgtt tcaacggaac
    31081 attccatact gggaaagttt cgaacttgga gtaaccagct gcatgtcctt tttggctttc
    31141 atgatacagt tggttcaagc aagtagcgag ctttccactt cctgggccag gagctgtcac
    31201 tacaacgatt ggtttagttg tttcaatata ctcatttttt ccaaaaccat cttctgagac
    31261 gattttttct acgttgactg gatagtcttc gatttctgag tgtttataga ccttgatgtt
    31321 tcgtctttct agtttattga taaagacttt cgtggcaggc tgtccactat agcgagtgat
    31381 aacgacactg ttgattaata agccataacc acgtaattca tcgattaacc gcaagatatc
    31441 catatcataa gtaatcccat aatcaccacg gatcttatta cgttcaatat ctccagcata
    31501 gacgcaaatc agtatttcag cttgatcttt caatttttgc agcaatttga ttttcgcatc
    31561 ttcttcaaat cctggaagta cacgtttcgc atgcttgtcg tcaactaact tcccaccaaa
    31621 ttctaaatat aatttgtcat agtggttcac tcgttccaaa atatacgctg actgctcttc
    31681 aatatatttt tctgaatcaa aaccaatttt tttcactagg ttccctcctt aagtactaaa
    31741 gcattttttc atttttttga ggattcgtca actggaagag cgaaattcct caaaaaaaca
    31801 aagaaaatgg tttaaaccaa cttgtctaga tagttattca ttggtatgat agtttttgat
    31861 aaggaaggtg ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa cactatatcc
    31921 gttcgatttt taccggttat gaaagaatgc ttgaagatcc cgaattggaa caattgacgt
    31981 ttacttatct aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc attcatttca
    32041 caacaagaga tctgcctagt aaataaaaaa taagataaag gtcatcggat tcgattctgt
    32101 cctatcagat ggaaagacta gttgatcgaa aaaaaggctg taccaaattt tggtgcagcc
    32161 tgttttatgg agcattgccc gctctttttt ataacaattt tttacagaaa atggtactat
    32221 tgaagtatta ataagtgaaa aacagatgag taagaggagg caagtagtat gaaagagcca
    32281 gatacaaatg caaattatga gccagacgaa ctagaggaga cgaacgcgga tcaggataca
    32341 ggacaagaat cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa tctttcccaa
    32401 ttattagaag acttacaggc ttttgaaaaa gctgtccgcg aagaagatat ccctgagatc
    32461 taccgtttat acaatggccg attgaatgaa gaattaaaaa gaagctcaaa tgaaaatcac
    32521 gagatcgatc aattgctgat gcgtaagatc catgatcgtt ttttacagga atttcctttt
    32581 atggaacaag tagaaaacat ctcgccaacg atgagctatt acaaaatcgg cacttattac
    32641 catgatcggc ccacggtcgg tattgatgca agtctgccag aaatttttgt tttgcctaag
    32701 atcgatgaag agtgggagaa atattcccaa ccggagtcaa cggactatga caaaaaaatc
    32761 aatgagctag acgcaaaggt gatcactgct cagacagaaa ttgaacgttt agatgaacaa
    32821 ataaaagaaa tcaatcgaca aatgactgat ttgaacgaca ataaaggatt cttgaatcgt
    32881 aaaaaaatcg aggaagaaat tcaagaatta gagaagaaga agcaagtact atctaatgaa
    32941 aaattaggct ggcttcctta tattgagacg cctgaaacaa tccagcagca aaaagaagca
    33001 ttgaagcaag aagcaagagc agaccagctt cgtgcagcca tcgtagaaaa agaacaaagg
    33061 caaatcagac gctatttcgg tagcaaagag ggattcggtc aagcaatcca tgaattcttg
    33121 atgaattatc ttggcaatga gaatcctaat aatcaatcta atgaaggagg ttccgagtat
    33181 gaatgaacac gatgaagcag aatggacaaa aaatgaagaa caaacagaag atcatgaaga
    33241 aacgaacact tcattggaag aacaaacaag taatcctctg gatgacacag aagaaatccc
    33301 tatgctagag gaaacaaaaa tacatgaaaa agaaaaagta actccgacga atacgaatac
    33361 tgcagaaaac acccatgctt acgaaataac tgaaaatccc gggtatttag aaggaaaaga
    33421 gcaagatcat caagatatcg tcagtttgat tgaaacagcg gaagcagaat tagcggattt
    33481 acgtatgaga aaagatttga tcgaatcaga ttttccacaa ttattagaat catatgatcg
    33541 ttttatattg aatgaagaac caatctccac gatcggaaag acaagcctga tcgaatactt
    33601 cacgtatgaa ttactgaaac cgttgaatga tattggtctg gaacggtcaa attcagctta
    33661 tgacacatgg aaaacaagac atttttcgat caattatacg ttgaatggac aaaacgaatt
    33721 agtcttttcc tttgtattac cgaccatcga tcaacgatac caagtggaat cgatgagtct
    33781 cctaaacgta tcaccagaga caatggaaat caatgtccaa gatgaccggg tgctttcttt
    33841 gatccgacac tggtcagtgg atcgagtatt ctctgcaggg cagatcacga tattcaatca
    33901 caaattgaat caattattag cccatgcaag aacacttggt tttaccgtta atcagacatt
    33961 actagacaat acgaagccac ttcgtttgaa tcttcagagc gaatttgaat tgacgaatga
    34021 agtattggat gatattttta tcgtggcaat gaaaaatcca tcctacgata tggaaaagat
    34081 caacgaaact acttatcaag tacgattaga taaaggacag tctttaacga taacaacaaa
    34141 cgaaaaagat caaacagtct tgtcgatctc atctggagac tacccacgat ctgtcattga
    34201 tttctttatc aattacgaat tcttggtccc attaatggtg agaaagtcct gattggataa
    34261 gttggattac cagatgtcat tcagataatt ctgttccaaa ggacttgcgg cagttatcca
    34321 gaataattgt cacaagtctt ttctgtatgt actaaatgga cgtgaataac ttgctgatga
    34381 aggagggatt tcattaggaa tgtccacaaa taaaatagca cagcttagct tattgtctgc
    34441 tgcttgtgta gcaggtagga tcgcttttca atttattccg aatttccagc caatgactgc
    34501 tattttttta tttatagtac tttacttaag cttaaaagat gcgttaattg tcatgagtct
    34561 ttctattgcg atcagcagtt tttattttgg tgttggacca tgggtcatcg gccaatggat
    34621 cagctatact gttgttctga gtttgttttc aatcgtctgc ttgagaaaaa cagtgcaaag
    34681 gaatctatgg ttaaagggag tctgtttttt tctggcgggc atggtttacg gaataggtat
    34741 gaccgttttt gatacgctgt tatatcgttt gcctcaacct tggatctatt accttcaagg
    34801 ggtgtcgttt gatttgatgc acagtatagg caatgttgtg ttctttcttg tttttctgcc
    34861 agtcgtcaaa cgtttttaca atcattcagg aggaaaaaat gaaaaaaata atttatagtt
    34921 tgcttgcaat gagtgtcagt atactcattt tgacaagttg cagtacaacc tatacacaat
    34981 cggcttccaa ccaaaaggag gaagtaaaaa catcgcaaaa agcaacaatc acgttacaag
    35041 aaaatggtca aaacttttcg gaaaaagaag tggtcttcaa aaaaggcgat gatttactga
    35101 gtattttgaa acgaaatttt gaagtcaaag aagataacgg cttcatcact tcgcttgaag
    35161 ggcactcaca ggatgagaaa aatcaactct attggatgtt tactgtggac gggaaaagtg
    35221 ctactactgg tgccaaagag atcaaattga atgatggaca atcggtcatt ttcaatttgt
    35281 ctaaactata agtttcataa aaaactgtta aagggcgaat attgccataa ttcagtgagt
    35341 tcagttgatg aaagaaggaa aataacatgg aaaattttaa aaaaagcgga atgttttccg
    35401 taattgctgc tctaggggct aatattctag ttgctatcag caaatttgtc ggctatgcaa
    35461 tcagtggcag tgccgccatg ctaaacgaaa gcatccacag tatcgttgat tgcagcaatc
    35521 agattttgct gttattcggt gataaacaag ccaataaagg acagagcgag ttgcatcaat
    35581 ttggtgaagg acgagcaaaa tactttttca gtacaatcgt cgcaatgatg cttttcttcg
    35641 gcggtggggc acttggggtg atggaagctt ttgaaaagtt gacgcatcca tcccatgaag
    35701 tgggtaatcc gatcattgtt atagcaatac tgttatttgg tttggtaatc gaaggaagtt
    35761 cgttacgcgt ggcaatgaaa gaaatcaaag aattgaatac tgaaaaactt tccacgatgc
    35821 gctttttgcg cgaaagtcga cacagtgaaa tactgattat ttttacggaa gacttgtgtg
    35881 ctgtaatcgg tttggtcttg gctttagcag gaacaatatt gacttatgca accgggattg
    35941 ctgcatttga tgcgatcagc ggattgctga tcggtttctt gctgatggga gccgccattt
    36001 tcctagcaaa agaattttat agtctgatca tcggtgaaag cgtgacagca agcgatttat
    36061 caaaaatcaa gttagctttt gaacgtcctg agatcagtcg gctgatcgat ataaaaacag
    36121 ttcatttaag tccaacggag attttagttg cagcaaaagc agatatcgtt ggtaacaaag
    36181 agaatgaagc ttactcggtc atcaatgata ttgaaaaaaa tatgcgtcag tctcttcctg
    36241 acaagaagat gtatatatac attgaaacag ataaatatga cccaaattat tctcgtaaat
    36301 aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa
    36361 aacggctaca atttagcgaa tttatgttac aatgagtccg ctgtttattt gttgtaatta
    36421 aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag
    36481 tgatgccact ttgtataagc tatataccag taggattagc ttgcggagta ttgttgcaaa
    36541 aagttggatt tgatcctctc ttatcaggac tgttatcgat tctggtcttt tccggtggcg
    36601 cgcagttttt agttgcatct atgcttacga cgcaagcatc atttgcaacg acattattga
    36661 tggttttctt tttagaatta cgctatacat tattaggagc aagtcttgcc ggttttatga
    36721 aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc actgaatgat gaaaactatg
    36781 cggtgaacta cttaaagttt tcaactgacc cttcctggaa taaaagaaag gctttatatg
    36841 tcaattggtt ctcaatgact gcatgggccg gtagcaatat gatcggtaat tttttcggct
    36901 cggtgattcg agtagatgct gaccttgtcc attttgcttt gacggccatg ttcattttca
    36961 tgtttatcat gcagatgaaa agcgcactac tgatctttac aggtttattc tcaggcgtat
    37021 taggggtcgt attcatggta ttattccaaa atactttcgg attgattgca gcaacagtca
    37081 ttgcttcatt tgccggattt atgctggaga aggcattcaa aaaagaaaaa gctaagaaac
    37141 taagagaccg cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa caggaggcca
    37201 atcaccatga ctaaactata tttgctactt ttagttgctt gtctgttcat cgtggcatat
    37261 atcccgcgct tgttcccgat gctttatttt actcatcgaa aagtgccatc ctggttcagt
    37321 gactggatga aatatgtacc ggtggcatta tttgcggcgt tagcttttaa agatgttttc
    37381 attactcatg aacatctgga tattgcttgg aatatcaaaa tagctgcaat gattctagta
    37441 gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag ttttgacagg acttgcgtct
    37501 gtctttttgc tgtctatgct ttaaactgtt ttattttatt aaaaacagag attgcgacat
    37561 aagttgttca aactgatcta tccgaactgc atttgcaaca gtcaattctg gatgcaagtt
    37621 tattcgtagg gataaaaaag attagtgttg gcttatgatg caagctcttt tttattttag
    37681 gaattagaga gggatttgtc cttctatgat tttttgctat aatagggaag atgtttattt
    37741 tatgaaagaa tagttgaggg aatttaaatg aataaaaaat tgatgcaagg aacattttgg
    37801 ctaacctttg ctaatcttct atgtaaagtt ttaggggtag tctatttgat tccctggcta
    37861 agtatgatgg ggaacaatca agatggtatg cttgctacga cattgtataa tgttggctat
    37921 cttccttatg gattgttcct gatgcttggt acagtcggat ttccaaatgc gattgcaaaa
    37981 aaagtagctg tagccacaaa agaaggagat aacgctgctt gtcggctgat ttttagaagt
    38041 acgatcaata tcatgtttgt catcggtatc gcctctgctg cactgatgta tttatttgct
    38101 cctttactag ccggaatcag tccgattgct aacgttaata atgggatact agctatcaga
    38161 agtttatgcc catctttgat tgctatccca attcttagtg caatgagagg atatttccaa
    38221 ggaaaaaatg atctgcggcc ttatggaact tctttgatta tcgaacaggc tgtgcgagtg
    38281 atcgttatct tggctggtac ttattacttg cgcgtattga ctgacggaac catattagaa
    38341 gctgtattga tcagtacagt cgcttctttc ttcggtgggt tagctgcgat tatccatatg
    38401 tatatcgtcg gacgcagaaa agacttcttc gaacttaaag attttttgat ttccagccgt
    38461 tatttcgaaa gagaaaaccg atctgcctct gtctctatca ttcgggaaac actaccgttt
    38521 atttttgtcg gctcagtcat tactatcgtt caaatgatcg accaagtaac gatgaaacca
    38581 ctgcttcact ttttccgtcc agagatcgca gatcagcaac tggaattctt attcagccgt
    38641 gcttctgtca atccaaataa attgacattg atcctgatct ccatggtagg aacagtagct
    38701 ataagcagct tgccaatatt aagcacgatg aagaaaaaag atcggataca aatcgaaaaa
    38761 acagtaggag atagtttttc gattgcgtta ttgattcttc ttccttcatt gactggaatg
    38821 tctttattag ctggaccact atacacattg ttttttggct atgatcctga aagtgtcgga
    38881 tatttccaaa tggctttgct agcttctttg tttttctctt tattcacgat cctatctacg
    38941 atgcttcaat ctttgaatca ccatcgtttt gcgatccgat taactgtaga agcaattatc
    39001 ctgaaagtca tcttccaagt aatcggatta ggattagttg gcggatacgg gatgagctta
    39061 tcaaatggct tagcattcgg tattgtcttt gttcgcggct atcactttat gtgcaaagaa
    39121 taccgaatag cacctttagc aaagatcagc aattttttct taaaaacttt ccgctccacc
    39181 ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg
    39241 gaatcaaaaa gctatgcaat gatttattgt gtggctgtag gaagtattgg cggcttagta
    39301 tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat
    39361 agcagaagca aagaataggt taactaatcg ttttttgctg tcacttgaaa aacggcaact
    39421 cgtatgatta cgcttgtcgt ttttctattt tccaatttta gtttcatatc taatcttact
    39481 tatgatatag ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa
    39541 cttgcagtag ttacagatag ttcggcatat ctgccggaac gtatcaagaa ccatcccgac
    39601 ttattcgtta ttccaatccc tttgattatg gatgggaaaa tttataacga aggaatcgat
    39661 atcgaagcgg atgaatatta tggactttta aaaaacagta aagattttcc tactacttca
    39721 caacctgtcg taggggaagt tttgggttta tatgaggaat tgaaagagaa agggtatgac
    39781 acgattttaa gtatccatct ttcttcaggg atttctggct ttgtcaacac attatttgcg
    39841 atgaaagatg acatcagcgg agtaacggtc attccttatg actctaagat cactagtatg
    39901 ccgatgggac acatggtaga agctgcctta gacttaaatg atgaaggtca gtcccttgat
    39961 gaaatacttg cacatatcga tcgaatccga gataatacct atgcttatct gattgtagat
    40021 gatttgaaca atttagtacg aggaggacgt ttgacaaatg gtgcagcgct gattggcagc
    40081 ttattaaaaa tcaaaccgat cctgacattt tctgaaggga aaattgtttt attcgaaaaa
    40141 atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa ttattggtag aagaaataac
    40201 gaaatacaac cgccagtcaa actttacgtg atacatgcaa ataatttaga agtagcgatt
    40261 gcggagaaag agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc
    40321 ggaccagtca ttggtacaca tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa
    40381 taattccttt tattcaaaaa acataccaaa aata
//
LOCUS       DARBFO010000015        36896 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000015 DARBFO010000000
VERSION     DARBFO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.016"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..490
                     /locus_tag="SCK51_000941"
     CDS             <1..490
                     /locus_tag="SCK51_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1251583.1"
                     /translation="ERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
     gene            complement(914..1084)
                     /locus_tag="SCK51_000942"
     CDS             complement(914..1084)
                     /locus_tag="SCK51_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1251584.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1278..1457)
                     /locus_tag="SCK51_000943"
     CDS             complement(1278..1457)
                     /locus_tag="SCK51_000943"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251585.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1467..1643)
                     /locus_tag="SCK51_000944"
     CDS             complement(1467..1643)
                     /locus_tag="SCK51_000944"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251586.1"
                     /translation="MITEVSYETLQKKKTLIVAAVLFPAPCVCRNCGSAVVDGNRKLM
                     VVKNKKKETIVHFE"
     gene            1844..2956
                     /locus_tag="SCK51_000945"
     CDS             1844..2956
                     /locus_tag="SCK51_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1251587.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3034..3327)
                     /locus_tag="SCK51_000946"
     CDS             complement(3034..3327)
                     /locus_tag="SCK51_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEG1251588.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3495..3809
                     /locus_tag="SCK51_000947"
     CDS             3495..3809
                     /locus_tag="SCK51_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEG1251589.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3830..5809
                     /locus_tag="SCK51_000948"
     CDS             3830..5809
                     /locus_tag="SCK51_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1251590.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5822..6148
                     /locus_tag="SCK51_000949"
     CDS             5822..6148
                     /locus_tag="SCK51_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEG1251591.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6164..6682
                     /locus_tag="SCK51_000950"
     CDS             6164..6682
                     /locus_tag="SCK51_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251592.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6682..8034
                     /gene="celB"
                     /locus_tag="SCK51_000951"
     CDS             6682..8034
                     /gene="celB"
                     /locus_tag="SCK51_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEG1251593.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8118..9623)
                     /locus_tag="SCK51_000952"
     CDS             complement(8118..9623)
                     /locus_tag="SCK51_000952"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEG1251594.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9715..10305)
                     /locus_tag="SCK51_000953"
     CDS             complement(9715..10305)
                     /locus_tag="SCK51_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1251595.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10484..12130
                     /locus_tag="SCK51_000954"
     CDS             10484..12130
                     /locus_tag="SCK51_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1251596.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12179..12898
                     /locus_tag="SCK51_000955"
     CDS             12179..12898
                     /locus_tag="SCK51_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEG1251597.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13377..14324
                     /locus_tag="SCK51_000956"
     CDS             13377..14324
                     /locus_tag="SCK51_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1251598.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14336..15256
                     /locus_tag="SCK51_000957"
     CDS             14336..15256
                     /locus_tag="SCK51_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1251599.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15297..16754
                     /locus_tag="SCK51_000958"
     CDS             15297..16754
                     /locus_tag="SCK51_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1251600.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17099..17713
                     /locus_tag="SCK51_000959"
     CDS             17099..17713
                     /locus_tag="SCK51_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1251601.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17706..19430
                     /locus_tag="SCK51_000960"
     CDS             17706..19430
                     /locus_tag="SCK51_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1251602.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19427..20878
                     /locus_tag="SCK51_000961"
     CDS             19427..20878
                     /locus_tag="SCK51_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1251603.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21351..23504)
                     /locus_tag="SCK51_000962"
     CDS             complement(21351..23504)
                     /locus_tag="SCK51_000962"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEG1251604.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23506..24552)
                     /locus_tag="SCK51_000963"
     CDS             complement(23506..24552)
                     /locus_tag="SCK51_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1251605.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            24702..25991
                     /locus_tag="SCK51_000964"
     CDS             24702..25991
                     /locus_tag="SCK51_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEG1251606.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26004..28706
                     /locus_tag="SCK51_000965"
     CDS             26004..28706
                     /locus_tag="SCK51_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEG1251607.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            28691..29548
                     /locus_tag="SCK51_000966"
     CDS             28691..29548
                     /locus_tag="SCK51_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1251608.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29619..30518)
                     /locus_tag="SCK51_000967"
     CDS             complement(29619..30518)
                     /locus_tag="SCK51_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG1251609.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            30687..32858
                     /locus_tag="SCK51_000968"
     CDS             30687..32858
                     /locus_tag="SCK51_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEG1251610.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            32926..33267
                     /locus_tag="SCK51_000969"
     CDS             32926..33267
                     /locus_tag="SCK51_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG1251611.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33543..35297)
                     /locus_tag="SCK51_000970"
     CDS             complement(33543..35297)
                     /locus_tag="SCK51_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1251612.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35308..36144)
                     /locus_tag="SCK51_000971"
     CDS             complement(35308..36144)
                     /locus_tag="SCK51_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG1251613.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36146..36829)
                     /locus_tag="SCK51_000972"
     CDS             complement(36146..36829)
                     /locus_tag="SCK51_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEG1251614.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        11907 a         6481 c         7537 g        10971 t
ORIGIN      
        1 cgaaagaagt cagacagcgg tggtagaatt gacgaaagaa aatcgactta caccaggcgg
       61 tgtcgtgttg acaaagatcg atgaccaaag cggagaagtc ctgcaaggag ctgtttttga
      121 gttacaagat gccaatggaa ccgttctgca gtcaggtcta accacaggag cagatggaaa
      181 actggcgata gatggcttag caccaggagc atatcagctg gtagaaactc aagcgccaac
      241 ggggtatgaa ttggatgcga caccagtaac tttcaagatc gaaaaagaac aagaagtggc
      301 gatcttttta acaaaagaga atagaaaagt tgctgatagc tcagatataa gaggaaacaa
      361 tccaacaagc agtggaaata agcatcttcc aaaaactgga gaaaccatca ttagccagtt
      421 tatattgtca atattgggtg tgctacttgt tttcatcagt ataaaattta gaaagaaacg
      481 aaatatatga gctaaaataa aaaattgaat aagcatagtt tttaaaatca actagtaccc
      541 atcaattgaa tggaaactac cgattttaga aggggtatac aaacaaatat tagtatcgga
      601 gtagagaagg ttgaacccaa ccagtaaata agtaaagaca attttgtgct gcgttgagaa
      661 gtgaaaaata aacctttgta gtaatactag ttaataaaaa atatacaaaa acactgcgaa
      721 aatcattctt gagtttcgca gtgttttttg cctaatgact caagtttatt gggtaatact
      781 ttgattttct ttctacaaat aatcagcgat cagttgtaat tttttctcta gttttaatgg
      841 ctctttgtca ataaggagaa aaacaagtcg ggtcttttgt ctcggaaagc ttcaaccaac
      901 tcgtgaataa aggttacgcc tccttgaaag gcgtggaaaa accaagtaat cgatcaatca
      961 ccaatttcgc atttggtaga acatgtttgg tgagctgaaa gtaggtggcg ttcatgtctg
     1021 taactaagaa ttctatttct ttaggattag tgcagctaag gaaatagttt gttaatcgag
     1081 gaattttttg tgtaggtaaa ttttctcgat taatttttct gtctcgccat ccgcggaaat
     1141 aaaactcagt ttatcctcaa cggaaacgtg agaacgaaat tcacaaccct caacactcaa
     1201 gacagaattc ttttggaaga ctttggtaaa tagcttctca actctttcaa tgtgcgaata
     1261 acggttgtca aagataccta acaacttttc gaaataaaaa ataacgacac tttttcagtc
     1321 agtaaagaag tgattttatg tctgacatga tttgaaatcg agtgccttgg acggataaaa
     1381 taactttgag cgttccaatg gcttcgacag tttttacagg tatagcgctg cttttttagg
     1441 cgtataacca ttggcatatg attatattat tcaaagtgga caatggtttc cttcttcttg
     1501 tttttcacca ccattaattt cctatttcca tctaccacag cagaaccaca atttctacag
     1561 acacatggag cagggaagag aactgcagca acgatcaatg tttttttctt ctgtaaggtt
     1621 tcgtaagaga cctctgtaat catcaaattt ttgtctattg atctcagaat ttttttgata
     1681 gaaccattca taaagcatat cgttctctca gttgtttatt ttgtggtgat ttaatcatac
     1741 aagaaaacga tatgtttttt tataaaaaca tgaataggaa aaatagttga caagaaaatg
     1801 acaacgccat atgatgttcc atagtattta caacctgtca tagatgactg taaaaaataa
     1861 aagtaaaaag aaaggacgac aacaggtgga tttttatcag tctctacaac tagatccgtt
     1921 tattttaaaa caaaaaatac gagaagcagc ttctaaaaaa gaaaagtgca tgtatatttg
     1981 ttcattgttt ttacgatcat tattcatcgt attgtttgcc atttgtttta tcattataat
     2041 tacaacatta tttgaatcga agcataaacc atacgctgtg gttttattct gtatgttgat
     2101 gagtattcgt tttgttgact ttggttataa gatttcccat tcgatcatag gtttagcaat
     2161 cgtcatgttc tcactattag tagcaccgta tgtccagctg atcaaatggt cagttatggg
     2221 catgttgatt cattttattt tactaagcag catattaatg gcaaccgcta gtgatcccaa
     2281 aatggggaat gcttctttgt acggcttttc atatctcttt attgtttatt cgttacctaa
     2341 agattcgttg aacaagaatt tctttacaca aacaagtagc ttattatttt tgtttttttg
     2401 ttggttttca gttctccttt acagaaaaca tcgagaaaag aacaaagaca aaagcttgtt
     2461 taaagagatt tttttgaaag gcatgtattc gcaggaaaaa atttggatgc tcatctatgc
     2521 atttggcatt agtcttttga tcgttgcagg agagtatgtc ccttttcagc gattgatgtg
     2581 ggcagggttt gcattttctt ctattgtatc tagctatgga ttgatgtcga ttggtttcaa
     2641 agaacgagca gtagaccgaa tcattggctc attgattgga tgtgttttat ttattggtat
     2701 cagccaattt attcctttta actgggtcgg tatattaggt ggtctagctt taggtgtttg
     2761 ttctacctat agatataaaa cagtttttaa ttcttttggt gctttggcaa tcactgccag
     2821 tttattcggg gttccaggag cagtaacgat ccgcattttt gagaatatcc ttggggtatg
     2881 tttaggaatc atgtatattg gggtgactga aatacttatc cgaaaaataa gagaaaaaca
     2941 cggtttgaat cattagatga gatcgaataa aaaataagga aaaagtgaag atacacatgg
     3001 atgataaatt gctatcattc catgtgtatc tttttacggt aaatcagtga aacgttcgac
     3061 tttcattttt aatttatact ttttacggag ttcgtttatt tgagccatca tttcggattt
     3121 gtgatgcttt tcgattgctt cttcatcttt ccaacggtcg atcagtaaaa gagattctgg
     3181 atcttctagt ggaaagtaat agtcatattt ctcgtttcct tcttctgcac gaatcgcttt
     3241 gacaatgttt ctttcgacca tttcttctgc aaattttctg gcttgtccat ttgtacttgt
     3301 atacaaaata ttcactgtga tactcactaa atcccctcct gaaaaaattc tatcactagt
     3361 atatcacggt gccagaaatt attattcaag atatgataca cattttttcc gcaatgctac
     3421 ggaaatatat attttgaata gaaaacgctt acaagatatg cttggtttat aaaaataaaa
     3481 ggagtgattg atttatgaaa aaagcattga ttatttgtgc ggcaggaatg tcatcctcga
     3541 tgatggcctc aaagacaacg gatttcttta agcaaaaaag agaagaggta ctcgtagatg
     3601 cagtttcagc tacagaaggc gataatatga ttaagaatag tgattttgat ttatttctga
     3661 tcagcccgca aacgacgatg tttttggata aattcaaaaa attaggagaa tctgttggaa
     3721 agcctgttgt tagtatccct ttccaagcgt atgtcccaat ccctacaggc atccagaagt
     3781 tagcagaggt gatcgaagag aacatatgat ggaataggag tggatgataa tgaaacagaa
     3841 ggaaaaattg ctgttgagat atttgattga tcacaagaag gagtatgtca ccagtcaacg
     3901 tttagcttct gaattgttat tatctgatcg gacaatccgt aattacttgc agcggatcaa
     3961 agagttagta gaaaaaaacg gaggtaagat cattgctaaa ccaggttatg gctaccaact
     4021 gcatatttta caaagactga catttgactt gtttttgagt cggcaagaaa tagtacctgg
     4081 atatgctaga gaaccgcaag gattttatga gtccgaagat cgacaaaagt atatattgaa
     4141 ccaattgctt ttggaagatg ccattctttt tatggatgat ttggcagaag agctttatat
     4201 cagtcgttcc agtttgacaa aggacatgca agaaatcaag gagcgattga ttccatattc
     4261 actaaaaatc gtttctaagc atggtcaagg gacatggatc gaaggggaag aaagaaatcg
     4321 acggcatttt atcatggata ctttttttgg aaaccattat ggaaattcat tgaaagaata
     4381 tttagggcaa agccgttttt tcccagacat cagttttgaa gagctagtca ttatcattct
     4441 cgatgaaaca agagaagcta agttaaaagt ttcggatttt atcatacaga atctgacttt
     4501 acacttggct ttggggatca aacggctccg agaagggttt gaaatcaagg aattaggcat
     4561 cgaacaagaa gtttctaagc gagtagagta tcaagtggcg cagcggatcg ttcaacgtat
     4621 tgaggtaatc gcaggtgtat cgtttcccga agaagaaatt gcttatttga cattacatct
     4681 catggctaag tcgaatcaca aagaaaatca aaatgatcag gaactatcaa aggaattagc
     4741 gaaagttctg aaaagattgt ctcaggaatt tcatttttca ttagtagagg atctccagct
     4801 tttcaatgga ctactcgatc atttgtcacc gatgatgatt cgattgagcc gaggtattcg
     4861 cattgagaac ccattgactg aggagataaa gaaagaaaat cccaaagtct ttgacgcagt
     4921 aaaaaggcat ttcagcaaca tgccggcttt gaaaaactat accatcaatg aagatgaatg
     4981 ggcctatttg gctttgcatc tcatggcggc attggaaaaa gaaagagctg cgcataaact
     5041 acatgcattg attatctgtg caacgggtta cggcagtgcc caactattaa agaatcgcgt
     5101 agtcagtgaa ttcggtaaaa atattacagt ggtaagtgta aaaggctatt atgagatcaa
     5161 tgaaacaact ttaaaaggaa tcgacctgat tatttcttct attgatcttt cgacaatgtt
     5221 tttcaaaatt ccagttttac atgtcagtat ttttttgaat gaagatgatg ttcgtaaaat
     5281 acgaaaggta gttggagaat ctatacccta tcattcctca gatagaccga taccagcatt
     5341 ttctctacag caaaaaaagc agatttacac cgaacagttg tctgaaaaat ttttcaagac
     5401 ttatacgtac gaaccgaaaa aggaagaaat tcttcatgat ttgctgcaaa agctgagcat
     5461 aaatgaagaa gaaggatata taagagaaat gcgaaagcag atccagcaac gtgaaaatat
     5521 gggacaaatc atattcagtg atacagtggc agtcccacat cccgctgttc cagttggaat
     5581 cgaaacaaag gtagccgttg cattgattcc ttctgggatg cagtgggatc aatatcaaga
     5641 aattcatttt gtttttttag tttcgccttc ttatgtcgaa aatgaaggaa ttacggtcgt
     5701 gactaaagca atcgttaggt tagttgacca gctaaaagta cagcaggaaa tcttagctga
     5761 acctattttt gagaatttca gcagtaaatt tatcaaactg atcgaataag gaggaaaaca
     5821 gatggctgaa aaaataaaca gtgaaacact acaagtgact tcttttgata tcattttcca
     5881 tagcggaaat gcacggacac tgatacatga agcatttcgc tcgatgcgta aagagatgtt
     5941 tgaagaagta gaagagaagt tgcgacaagc aaatgaagag attcttcatg cgcatcagtc
     6001 acaaactcaa cttctgaaag aatatgccag cgggacaaaa atagaaatgg aaattatcat
     6061 ggtccatgca caagatcatc tgatgacgac gatgacatta ttggaagttg cacaggaaat
     6121 gaattatttg tatcaaaaaa ataaatagaa aggaaggatc attgtgcagg atacagtaaa
     6181 aaggaaaatc gaattagaaa aaaagcaact tagtttgaaa aacatgtatt tcaatcgtta
     6241 tctaatcgta cgttacctga ctgccttttt ctttttcatg aacatgcaat ggatgattct
     6301 gcttctatca gctaaaagtt taggaagtag tttaccgatg attctgattt tagctatttt
     6361 gcctgcagta ggagaacaag taaagcttta tcgaaaacac caaacgaacg ttccatggac
     6421 aaagtggtat tttttgttcc agagtgtctt taatttatta gtgattccag tgttgtttac
     6481 ctcaggattt acattcttgt atccttttat ggcaaataac aatggaggac agttatttgt
     6541 tttcattctt gttgtaagcg gtatttttgc cagtgcattt atacagtatc gtttaaagaa
     6601 aatttcactc aaccaagacc aacagtatat ccgtattaaa caatatgaaa aagcgttata
     6661 tttaggaaag gagaataact aatgtttgat tttttgcaga aatacctgat gggacctatg
     6721 gggaaaattg cgcaacttaa aatcgttcgg gctgtcatgg cagcggggat ggcttctatt
     6781 cctttcacga ttgtaggctc gatgtttttg gttttaaata ttttgccatt accgttccca
     6841 tttttagaag gattttttaa tgcaactttt ttcaaagtta gtgacttgta catgatcgtc
     6901 aatacaatga cgatggggat tttatctatt tactttgcca ttgtgtttgc ttatgaatta
     6961 acgtcaattg aacgagatga acaaaaatta aatgttaatc cgctcacggg ggcattacta
     7021 tctgtttttg cattctttat gtgtatccct gaattgatca tgactgatgg taaaatcagc
     7081 ttgattgctt ctatcaatga gacagaaact gtagtaaatg gtattaggat gggctctttt
     7141 gtcgaacgtt taggaacttc aggaatcttt acagctatta ttatgtcgat tattgcggtc
     7201 gaattgtatt gtatgtgtgt caagagaaat tgggtcataa aaatgccaga tgttgttccg
     7261 ccaggagttt ctcgttcttt cactgctctg atcccaacat ttgtcattgc gtttgtggtg
     7321 atgatcatca atggattatt agttgcacta ggaacggata tcttcaaaat gattgctatc
     7381 ccatttgggt ttgttacgca gttgacgaat acatggcttg gtgtcatggt gatttatttc
     7441 ttgatccatg cattgtggat cgtagggatc catggagcga atatcatcac ttctttcttg
     7501 accccgatcg tattagctaa tatggcagct aacgcacaag gcgcaaatta tccgttggca
     7561 ggagaattca acaattctta tgtaaccgtt ggtggatcag gtgcgacttt aggattgatc
     7621 atctttattg cattcctcgc gaaatctgat cagctgaaag tgttaggaaa agcctcactt
     7681 gtaccaggat tattcaatat caatgaacca atcattttcg ggatgccaat cgtctacaat
     7741 ccctatttag cggttccgtt tttcttagct ccgatggctt ctgctacact cgcttatttt
     7801 gccattcggc tggaaatcgt caaaccgatg cttgcacaga tgccgtggcc ttcaccagtc
     7861 gggatcggag catttgttgg tagtggtggt gattggaaag ctgctgtgtt agcagtcatt
     7921 tgtgcaatca tcgcattcat tatctggttc ccatttatca aattctatga taataaatta
     7981 ttaaatgaag aaagagaaaa ggcagcagaa ttagctagtg aaaagttaaa atgatttcta
     8041 agaaaaaagg aactgtgaca aaaaatcaca gtcccttttt ttcttagaaa tgggaaacca
     8101 ctagtatata aataccatta ttcttcaatc aaccgcttca cgattgcata atccccttca
     8161 taaggttcat ctacaccatt gattttttga tagggatcca cgccttttcc cgcaataacg
     8221 actgaatctt gcggagcgct ttctgctaaa gcgcgtcgaa tcgcttcagg acgatccatt
     8281 tcataatgaa cgatcaaatc tggattagta attgcttcat tgatttcttc cgcgattttt
     8341 ttcggatctt caaatgctgg gtcatctgcc gtcaaaaagg cgatgtcagc tgtttctgat
     8401 agcacttttc caaaatcatg tcgacgggag attgctttgt ttcctgggct gcctaataca
     8461 acaattacac ggccatctgg atgttcattt ttggcaaatt tcagcaatgt tttcaagctt
     8521 agataattat gtgcatagtc cacatagaca tgcgctccat tggattggat caattgatcc
     8581 attcggcctg gtacacgagc ttcttttaat ccgtcttgtg catcttgact ggaggcacct
     8641 gttagtgctg cggcaatgat cgcactggct gcattttctt ggttgaatcc tccagccagc
     8701 aaaatatcat aagcagtatc gcagatagct aagcgatctt catgagaaga taaagtaaaa
     8761 ccgtgagtac ctttatctga acggatcact tgataattgc tttcttccga acgaccataa
     8821 gtgatcgttg gaatctggaa tagatcggct gtttccctaa gtaaatcaaa atagtcactt
     8881 tcatgattta aaacaactgt ttttgaattc aagatcaatt gtcgcttgca atagaaatag
     8941 tcatcaaatg tcggatgttc gatcggactg atatggtcag gcgagatatt caagaagatt
     9001 ccgacatcta aagtcaagcc gtatacgcgt tgtgttttgt aagcttgtga agaaacttcc
     9061 atcactagat gagtcatacc attttcaact gcttctgaca tcatacgata taaatctaat
     9121 gactcaggcg ttgtcaaatg ggatttgaaa tatgttttgc catctaacgt tgtattcata
     9181 gtagaaagca atgctgtttt tttatttgtt gtgtgatcca agattgcttt tgtaaagtac
     9241 gcagcagttg ttttaccttt tgttccagtg aatccgatca ctttcaattt attttgcgga
     9301 tagtcgtaaa aagccatagc aagaagcgcc attgctttac ggatatccgt tacgatgatt
     9361 cctactgctg aattttcgta tggttgttct gatacataat attgaatacc ctgttcgatt
     9421 gctgaatcca aatattcttc tttgaaattc attcctttac agaaaaacaa agtagaagca
     9481 tcagcttttc ttgaatcata acttaactga agaaattcag ctttttctgg tacatcaaga
     9541 tgccagcctc ttgttgaaac aaattctttt aatagatttt cttttaatag taaatgacgg
     9601 atgtcttcta aagagaagct cattgattga tccctcgctt tattcaattt cttggtttat
     9661 tatagcacac tctttttaat atatcgtttg tttccgctat gacaaaatcc agctttattc
     9721 aaattttctt gacacagggg acaagcggat gagtattttc ataaacaatg aactgactgt
     9781 aatagatagg agaacgaata gtgtctccaa caatccaaaa acataataat tcaaaaagac
     9841 gataagacta ttggtaagta aaagtccttg cggcattgga agccgaaaaa atttgtggaa
     9901 aataatcggc ggtatcgtta taccgccatt tgaggccccg actgtgtaca atgtgtaaac
     9961 cccaagactg aacaaaagac ttcctgaaat cagtgaaaag aaatgtgagg agatcatttc
    10021 cacttttgga acaatctcta aaacaacagg aaaaagcaga cttcccgcta cagtcttaaa
    10081 aaatactgat tttcctaata ggacagcggc tagaagcagc atcataagat tgatgctcca
    10141 aacagtaact gagcgttgga tagaaaaggc cgtttcaact aaatgtccga tggcgcccac
    10201 tccggcggaa gctatatcgt gagggagcaa gaaccaattc aatccaactg tgataaagaa
    10261 aagacctaaa aatataacgg gtagtttttt tatttcttgt gtcattgata tctcctataa
    10321 atgttagcca taaaaggctg tatatttctt tactttacca aaaaaacgaa accgaattca
    10381 aaaaaacaat tgttcttata aaagttatat aaaatcgatt gccagtttct gttttaccaa
    10441 ttaaaatgat agtaatgttt tgtagaaagt tggaatctga ccgatggata atcaaagaaa
    10501 accagcatta gatcaattaa caccgcaaga aaaaatggca cgaggctctg cttggatgac
    10561 cgccagtaat atcatttcaa gattgctagg cgctgtctat atcattccct ggtatgcttg
    10621 gatgggcgaa aatgctaagg ctgccaatgg attattcaat atgggataca atatatatgc
    10681 attgtttcta ctgatctcta cagcaggaat acctgctgcg attgcaaaac aaactgcacg
    10741 ctataattca ttgaatgaat atggcacttc acgacgatta tttatccgtg cattacagat
    10801 gatgggcggg ttaggcctgt tgtttgcttt gttcatgtac atcgcctctc cttggctagc
    10861 tcatgcttct ggtggcggag aagaactgat tccaacgatg cgttctttga gtatcgctgt
    10921 attggttttt ccttgtatga gtgtgatacg agggtatttg caaggcaatc aagaaatgat
    10981 gccttatgcg ctgtcgcaga ttgtagaaca ggtggcacgt attttttata tgctattggc
    11041 tacattcatc atcatgaaag tcatggatgg tgactatgtg acggcggtca cgcaatcaac
    11101 tttcgcggca tttatcggta tgctagcaag tattcttgtc cttttgtatt ttttgaagaa
    11161 acatcaagcg tatacatcgg cgtttattca ctacagcgaa aacaaaataa atattgcaac
    11221 gaaggaattg ttgttagata ccgttaaaga agcgattccg tttattattg tgggttcagg
    11281 ggtaacgatc ttcaaattag tcgatcagtt cacttttatg aagatcatgt ctgcatccac
    11341 cgagtattcc aatgcacagt tactggatct tttttcgatt tttagtgcca atccagataa
    11401 gttgacgatg gtagttattg cattggcaac atcgatcgcg tctactgggt taccgttgat
    11461 tacagaagct gttacgttga aagatcgagt aggtctggca aaattgacaa gcggaaattt
    11521 acagttgttt tcatttttta tgtttccggc gacatttggt gttatgttac ttgcctatcc
    11581 gctaaataca ttattttata caccggattc tctaggaagt aatgtactga tccaagcaag
    11641 ttttgtagga ctcttcctag gactatatat gctagtttcc aacatgctgc aaggaatgtt
    11701 tgaaaacaag gcggcaatta attatttggt tattgggttt ctagtgaaac tccttcttca
    11761 atacccagcc attcgtttat ttgaagtata cggcccttta ttggcgacaa tgatcggatt
    11821 tgctgtgtct tgtaccttga tcttaaagag aatccatgaa gtagcccgct ttaaaccaag
    11881 ttttgtctgg cgcaggacat tgctgatctt tattcttacc ttgattatgc ttttggcttc
    11941 gtggatcaca aagatgattt ttggtgcttt tttaaacgaa gatagcaaat tccagtctct
    12001 attgttgatt cttgtagtag caggtatagg ggctttagtg tatggttatt tagctttgaa
    12061 aatccgcttg gcagaaagac tactaggacc aggaatagcg cgtttacgac ataaattaaa
    12121 aataaaataa gtgaatcaga agagctagat tgatgctctt ttgaaatggg ggaaacgcat
    12181 gcgtttagat aagtttttag cagatatggg acttggcagc cgcaaagaag tcaaaggatt
    12241 aatcaaaaaa ggaacgatcg aagtcaatgg acaagtggtg aaaagcgata aagcgcaagt
    12301 aaatgaattc gaagatgcgg ttacttattt aggagaaatc attacgtatc aaaaagattt
    12361 ttactacatt ttgcataaac ctgcaggagt catttctgct actcaggata atcatgatca
    12421 aaccgtaatc gatttattgg aagaccagga ttatcgggaa gatctttttc cagtcggccg
    12481 attagataaa gacacagagg gcctcttgat tttaacgaat gacggggtgt ttgcccatca
    12541 gttactatca cctaaaaaac atgtggagaa agaatatcta gctgaggtta gcggtataat
    12601 gacagaagaa gatatccgtt tatttgcaga aggactgatt attgatggcg atgagcagac
    12661 attgccggca aaactccaaa ttgatcaagt agatcaagag acagaaactt caattatccg
    12721 tctgattttg catgaaggaa agtttcacca agttaagcga atggtcaaag cagtaggaaa
    12781 agaagtctta tatttaaaaa gaatccgtat gggaaaattt tggctgccta aagatttgaa
    12841 taaagggcaa tatcgaccca tgaccaaaga agagctagaa caagtgagag aaaaataatt
    12901 gaatggatag ctaaaagaat aagcagagta gttgtagcgc catctgaaaa gcagacttgt
    12961 ccgtctgact ttcggatggc ttttttgtga aatttcggct tatttccgta gaagctactt
    13021 ctggaacacc gtttgttcgg aataagggac tgtgacaaga cttttgttac agtccccatt
    13081 gttccttgtg actcagagtt agatagtaag gacacgatct ccctaatact caggccaaaa
    13141 cgctgaattc acaacctctg ataaacggtg ttcaatcagc tgaccctcgg tattagctca
    13201 aaaaacggca aaatatgagg aactattttc ccgttttttt ctctaatact caggccaaaa
    13261 cgctgatttc acaacctctc ctatactttg tttcattttc cctatacttt gtttatttat
    13321 tctcattatt gaaagcgcta taattacgtt gcgaatgaat cgaaaggggt ttttagatga
    13381 aaaaacgaaa ggggttcaat cattttttta tgaatctatg gcgttataaa gcacttgtcc
    13441 tgatggctgt accgggtatg ctttggatga tttttttctt ttacatacct gtaatggcga
    13501 atgtcgtagc attcaaaaat tttcatattt cagcagatgg gttcttggct agcttgaggg
    13561 aaagtccttg ggtgggttgg gaaaatttca aatttttatt ttcttcggat caagcatttc
    13621 tcatcactaa aaatacaatt ttatataatg tagcatttat ccttttgaat ctgctgatct
    13681 cagtcgtatt tgcaatcatt atgagcgagt tgagaaataa acgactcgtt aaagtttatc
    13741 aaacgatgtc cttactacct tattttctat cttgggtcat tatcagttac tttgtttacg
    13801 cttttttaag tcctgacaaa ggaatattca atcagtggat cactggacat ggaggagaag
    13861 ctgtcaattg gtataatgaa ccgaaatatt ggccgtttat cttggtgttc attggtacat
    13921 ggaaagggat tggttataac agcattattt actttgcatc tgttatggga atcgatccga
    13981 cttattatga agctgctatg gttgatggag ctagcaaatg gcagcagatc aaacatgtga
    14041 cgataccaca acttattcca ctaatgacga ttttaacgat tttagccgta ggaaacattt
    14101 tccgagctga ttttggcctg ttttataaca ttcctaggaa ttccggtgca ttatatgatg
    14161 tgactcaagt tttggatacg tatatctaca atggcctgac atccacagga gattttggta
    14221 tgacggctgc tgcaggactt taccaatcag ttgtcggctt tgtcttgttg atgatcacaa
    14281 atacgatcgc ccgacgtttt gatagcgatt ctgcattatt ctaaggagga aaaaaatgaa
    14341 aaaaaagaaa gtggagagag tccaagtccg ctctttcaat aagacggcta attttttctc
    14401 taatataatg attgcactct ttgctatttc ctgtattttg ccatttatat ttgttatcgc
    14461 catctctttt accgatgagt ccatattgtc gactcacggg tacagttttt ggccagaaaa
    14521 gttcagtata tttggttata cgtacttgtt cgaccagatg caggataaaa ttttccaagc
    14581 gcttttcgtc actgtacttg ttactgtttt aggtacactg atcaatagca cggctacttc
    14641 tttatatgct tatgcgattt cacggtcaaa ctttccattc cgtcgttttt ttaccgtttt
    14701 ttgtttgatt acgatgttgt tctcaccagg gatggtggcc aattatcttg ttatgacaaa
    14761 tcttcttcag ttaaaagata cgatttgggc attgatcttg ccaatggctg tcagtccctt
    14821 caatattatt gtgatgcgca cattctttaa acgttcggtc tctgattcga ttattgaatc
    14881 ggcacggatc gatggagcta gcgaactgcg aatatttgtt cagatcgttt taccgttagc
    14941 tgttccaggt attgcgacaa tcagtttatt tgccgcattg ggttactgga atgactggtt
    15001 caatgcttta ctatacatcc aagatgataa gctagttcca ctacagtatc ttttgatgaa
    15061 aatccaaagt aatattcaat atctgacaca aaattcagga gcaggaagcc aattgacagg
    15121 cggattagcc tcagttccag gagaatctgc cagaatggcg atcgtagtca tctcgacgtt
    15181 gcctattgcg atcagctatc catttttcca gaagcatttt gtcaaaggtc taacaatcgg
    15241 cggagtaaaa gaataaagaa acaaaaatat aaatcataaa attaggagga agaaaaatga
    15301 agttatggaa gaaactagcc tttacaggtg tttcagcgtt gttgttggga acattagcag
    15361 catgcggagg ttctggttct aaagaccagg cagaagctag caattcggac actttgcaga
    15421 tgtatcaaat cggagataaa ccagataatt tcgatcaatt gatggaagta gcaaacaaac
    15481 gaatcgaaaa agagatcggt gtaaaagtca atatcaatta cattggctgg ggcgattacg
    15541 aaaagaaaat gaacgtcatc atttcttccg gggaaaatta tgatatcgcc tttgcgaaca
    15601 actatgtttc caatgcccaa aaaggagcct ttgctgattt gactgaatta gcgcctaagt
    15661 acgctgaaaa agcttataat gatttagatg aagcatatat taaaggaaat ctggtcaatg
    15721 gaaaattata tgctttccca gtcaatggaa atgtctttgc tcaacaagta cttactttta
    15781 acaaagcttt actagataaa tacgatcttt caattgatgg aatccaatct tacgctgatg
    15841 cagaaaaagt cttgaaggaa ttccatgaaa aagaaccaaa cacggcagct tttgccattg
    15901 gacaaggctt caaagtccaa gggaactttg attatccttt agggaatacg ttaccatttg
    15961 ctattgatct ggatggagat gattcgaaaa tcatcaacca atacgataat gaaacgtata
    16021 tcggactgtt acgtacaatg catagctggt acaaacaagg actcattcca agtgacgctg
    16081 caacgagcaa taaggattat ccactagaag gaaatacttg gctaatgcgt ggagaaacac
    16141 aaggaccgta tgattatggc gatacaattt tgacaaatgc agcaaaacaa gaacttgtct
    16201 caaaagcaat tactgttcca ctaaaatcta cagctcaagc acaaatggca aattttgtcg
    16261 tatcaaatac ttcaaaaaat aaagaaaaat cagtagaact tctagggctg cttaacagtg
    16321 atccagaatt attgaatggg cttgtttggg ggatcgaggg agaagcatgg gaaaaagtcg
    16381 atggttcaga tgataaaatc aaattgctag atggttatca gccaaataca catatgtctg
    16441 cttggaatac aggaaacaac aagattctat atacacaaga gtctattacg gatgacatga
    16501 ttgccaaacg tgatcaatcg attgctgatg cagaaacttc accgatttta ggattcagct
    16561 tcaacactga cagtgtcaaa acagaattaa gtaatatttc taatgtcatg aatcaatatt
    16621 tagatggatt gaatacaggg actgttgatc cagatgaaac attaccgaaa ttgaaagacg
    16681 ctttggacaa ggctggctat gataaggtat tgaaagaaat gcaaaaacaa tatgatgagt
    16741 tccgacaaga ataataatga ataaagacaa aggggttgtg acaaaagacg tgtcacaacc
    16801 tcttgttccg aataaacggt gttcaaaagc cagtttctcg gtattaagct aaatatatgt
    16861 aaaaatgtgg aaagccatat tcacaaaatt tttcttacta ctcgaaacta agcggctatt
    16921 tcacaaactc ttggtaaacg gtgttcaatc agctaaccct cggtattcgc tcacaaaacg
    16981 acaaaatatg aggaactatt tttccgtttg tttctctaat aatcaggtca aggtgctgat
    17041 tgcccaacct ttttttgtat aatgagaaag tataaactta atgtttggag gagaagatat
    17101 gttagggaag gttttggaag agttatttat ccggatctgg gtcattataa aactaacctt
    17161 gtatttctgg atatatacat ttgccggcgg gatcatcttt ggattaggtg cggcatggaa
    17221 aacagtcaat gaactatttt atttgtacgg attcgagtat aaagaaatca caataaaaag
    17281 aggctggaat atatataaaa gaaatttttt aagaggcaat ctgctattta gtttattttt
    17341 atctggtact gctttattga gttacaattt atatttatct gttcaaatca aagggctcat
    17401 ttttttagct attgatttta ttttactttt tgccttgctc tgcctatttg ctacttatca
    17461 atacagtatg attttagata gtgaatatac gatctctgtt cctaatctgc taaagctaag
    17521 ttttatttcg gttttttcca gcttttcgtc ttttctaaaa ataattattg gtagcggtat
    17581 aattttaggc gtgacttggc aattcaaagg attgatcctg tttggtgtca ttggtctttt
    17641 gacggtttgg aatggtacga tgacaacaca ttggcgtgaa gaattagata aacagttgga
    17701 aagttatgaa taagtttcgg attctatttt caaaaggaac cttactaaat cgtctgttgc
    17761 ggaaatacgc ggttatcatg atcggtgtaa cgatgactgc taccgttatt tttagtatcc
    17821 atacttggga acaaaatcaa aaacaggcag aaaacatgac aagtgatgcc gttcaaagca
    17881 caagcagaat gctgaatgat aaaacaacct tgagtaggat catcaaaaac cagcttgtag
    17941 ggaattccga aaagatcgaa aatgtgacga cctacttaac aaaacccata gatcagtatc
    18001 tgatgtatgt atacgaacag caaaacagta ccgatgaatt agtttctttc ccaaatcaaa
    18061 tcaaagatct ctacgcaaat tatgaagaac taagtgcgat atatatcgtg ttgaatcaac
    18121 taccggagta ttatgaatct tctagagaaa acaaaggtgg ggaaataaaa aaggggacac
    18181 caaaattact agacgccttt tatatcaagt tgcctatcac acaaggagga gctgagtcag
    18241 gaagtatttt tattggtttt caaaagaaag aactagatat gattttagaa aatctaacgt
    18301 cattcaatgg actctcttta tacatgattt ctggaacgac aaatcgacta tacacattcc
    18361 atgataataa aatagctaaa gaaacgtttg aaagacaaga aatgatcatc acagagagtc
    18421 tgaagaaaaa ttcacaattg ccgattgacg atctggaaaa aaacaatttt gttcaacacc
    18481 aagaactgtc tgatgattat cgtatattag ctgtactaga taaaaaaagt gtaaaaatcg
    18541 aaacgggaaa aaatcttgcc ccgctgatca ttggtatcat tattttagac aatatactgc
    18601 ttatctttct ttataaaaca ttcaaacgat attcacagca agtatctatc gtcatgcagg
    18661 ccatggatca aacagcagca gggaatttag agacgcggat cgacataaca aacacggaat
    18721 atgaactgag ggaactttca atcggtatca acgaaatgtt ggatagcatc aatcagtttg
    18781 tagaagacat ctacaaatta gaaatcaaac agcaagacgc acatatgcgg gcgcttcaag
    18841 cacagatcaa tccacacttt ttatataata cattagaata tatccgaatg tatgcaatca
    18901 gcgaaggaag tgaagaatta gcagatgttg tgtatgcgtt ttccgctttg cttcgaaaca
    18961 atacgaatca agagaaaacg atcactttaa aagaagaact ggacttttgc gaaaaatatg
    19021 tctatttgta tcaaatgcgt tatcctaatc gagtagccta tcattttatg atcgatcctg
    19081 atttggaaaa gatcgaagta cctaaatttg tgatccagcc attagttgaa aattatttta
    19141 aacacggtat tgattttact cgattcgata atgcgctaag tgtcaaagtc ttgcaggaag
    19201 gcaaacgggt aaggatcatc atcaaagata atggaaaagg gatgactgaa aaacggctaa
    19261 aacagattga agaaaaactg tctcatccta aagtggaact tcacggttct atcggtctgc
    19321 aaaacgtgaa tgaacgttta cgtgccagct tcggcagcag ttattatatg tcgcttgaga
    19381 ataatgagac aggtggactg accgtttcta ttacattcaa ggagggatga gcgtgtatca
    19441 agtgttattg gtagatgatg aatacatgat tttgaatggg ttaaaaaaga taattgattg
    19501 gcaaagttta ggctttcaaa tagtagcaac agcagagaat gccaaagaag gactagctgt
    19561 acttgaacag cgacagattg atttagtagt aacggatgtg acaatgccag aaataaatgg
    19621 actggaattt attgaagcgg ctcaaaaaga acgtcataat tttgaattta tgattttatc
    19681 aggataccaa gaattcgatt atttgaaagg aggtatgcaa ttaggcgccg tgaattattt
    19741 gatgaaaccc gtgaataagt tcgaattggt tgaaagcctc aagaaaatca aaacacggtt
    19801 agatcagcaa aatgaacaaa aaaatcaaca agaaatctat caagaactgt tattcagtca
    19861 atggttgaat gaagaattgg atgaaataag tgaagaagaa ataataggac aaataagtga
    19921 gaagcagcat aggatagtat tgattcaact ggcaagaact catgatactc ttatcaatag
    19981 atggttgcaa gaacggcaac aaatctttta ctatcaacga aattacggag accgtgtatt
    20041 gatcacgttg ttgttggagg cagatgaagt tgatggcttc tgttgttttg ttcaagaaaa
    20101 ctttcctgat caggaatggc tgatcagtat cggggaagag acagattctt tggacaaaat
    20161 ccctgaaaca ttccaacaag cgaaagacaa tctacagttg catcaatttt atggagataa
    20221 agacgagcat gttttttacg cagtacaagc agaaacaaaa gatcaaacca tcgatttttc
    20281 aagtttcaga cgcgtgctgc aaaataaaca attggaagtc gctcaaaaaa tgatcactga
    20341 tttttttgaa cagtttcagt tagcagccat gatgccggaa gatatacgat attccagttt
    20401 tttactgttt atggagattc aacgggaatt gattgatctg gaagatgagg aatatttaca
    20461 aggaatcgaa aaaatccatc aggcaaaaac ggtgcaagaa cttcagcagc tacttctgtc
    20521 attcatcaaa aagcatcaga gacagaaaaa gtacagtgac aatgtggaaa aagtaatcgt
    20581 aatcctgcac cagcattatc aagaaccact tacactcaaa gaagtgtcag aaaacctgca
    20641 tttaaatgtc atgtatctag gacagctgtt caaaaaagaa acgaaaaaaa gtttttcagc
    20701 ttatttgaac catttacgta tggaaaaagc gaaacaactg cttttgcaca gtaatcaaaa
    20761 catcaatgag atagcaagtg agattggcta caacaacacc acgtatttct ctaaattatt
    20821 caagaaaatc gtcggcagat cccctaaaga gtaccgagaa aacagtggag aatcataaaa
    20881 gaaaaagcca atgtaatgaa ataacttata aaaaattgtc tagaaatcta attttgggct
    20941 gttcttatta cttgaggcaa ggcagctttt tcatgacctc tcgataaacg gagttcaaaa
    21001 gccagtttct cggtattaag ccaaatttat gcaaaaatgt ggaaagccat tttcacaaaa
    21061 tttttcttaa tactcgaaac taaacagctt tttcacgacc tctccactaa acggtgttca
    21121 atcagctgac cctcggtatt agctcaaaaa atggcaaaat atgaggaact attttgccat
    21181 ttgtctctct aatactcagg tcagaacgct gatttcacaa cctctcaata aacggtgttc
    21241 aaaagcttgc atctgcggta ataagaccaa ccaaacgaaa aatatgagga tctatttttg
    21301 cttggctgtt cttattactt gatgcaggac agctttttca caacctcttg ttaattatag
    21361 tttgataagg aaaaaggcaa atcttttgct tgatatttct tagggtgagg gatgatccct
    21421 aattggtaag agatcgtgcc gcctttcagc aaatcttgat gggtaaaata taatttcgta
    21481 tattctctgt ccatccgttt gattttatgg ataaattgtt gctgcggccg attaggcgtt
    21541 gcttcaatcg tcaattcctt tccatttgac aaaagcaaaa cagccttgtc aattaatggc
    21601 ataccgataa catattcacc ggttccaggc gtgacaggat aaaatcccag actattgaag
    21661 atataccaag cggccatact tccattgtcc tcatctccag ggtaacccat tggagaatcg
    21721 tcgaaggtct gagtcatcaa ctgtttaagc agtggctgtg ccatttcagg ttgtcctata
    21781 taactgaaca agtaaggata gtgaaaactt ggctggtttg aaatagctaa ttgtccaaat
    21841 tctatcgcgg ccatctcact catttcgtgg atctcaaaac catatcctcc tgggtcaaac
    21901 gttgggggct gattgcacag ctcaatgagt ttttctttat atctctcact gccaccatat
    21961 tttccgatca gtcctgaaaa atcgtgataa acagcaaagc tgctttgcca agcacttcct
    22021 tctgcataat cttgtcccca acgattagct gaaaatggtt ctctgaactt gccatcttta
    22081 tcttttgaac gcataaatcc tgactcatag tcaaagatat tttgatagtt caatgattgc
    22141 tgctgatatt tcttcgtcat ttcctcttct tctagtacct tggcaacttg gctaatacaa
    22201 aaatcgctat aagcatagtc taatgtgtga ttgactgatt cgtggtagtg attaggaaca
    22261 tagccatatg tcaagtaatc ttctgttcct tggcgtccat aatttggatg tttgctttga
    22321 accgtagcac ctttttccat tgccgttaga aattcaggca tcaagtctcg tcggatatct
    22381 tttacagcag catctgcaat gaccgcatcg atcaatgtcc caggcataag tccacgttca
    22441 tcaggagaca gccattttgg caaatagcca ctttctctat agctgttcaa aaaaccttcc
    22501 agcatttctt cgtatttttc tacagcaatc aaagaataaa gaggataaac cgttttatac
    22561 gtatcccaaa acccattatt tgtatagaga acaccaggtt ttatagatcg actagtcgta
    22621 tcatagtgga ttttttgccc actaggatca atttcataaa atgtttgtgg aaataaaaac
    22681 acccgatata aattatgata aaacgtccgc aattgttgtt gatcatggtg tgtgagctgt
    22741 atacgatcaa aataggactg ccattgttct tgactgtgag caaggtaagt ctctttgctt
    22801 tggttttgtt ctagttccag gttataagct gcttgttccg agctgataaa agatgtccca
    22861 aatcggatag tttgtttttt tgtctgttca aaatacaacg ggatcgagtc gctatttttg
    22921 ttggaaacag ctaactcatc aattgttaaa ggatattcaa atctcaatat aaaataaaaa
    22981 gaaaaatcag gatcttctga accagcaaaa tgtgtgattt ttccttccac tgtgtaagga
    23041 tctataacaa agagctgatg gcgtcccgga aaggataaaa gtaaaccgct gtcatttttg
    23101 ctgtaatcaa tagataagat tccgccatac atgctaggta tcaatgtaga acagatgcca
    23161 taacgtaatt gacagatcga cagatgagtc gggcaaaata tactttcttc tgggcggtaa
    23221 gaactttgag catggaaaag ggtgttctcc tgtagttttc cattgatcgg cgtaaacagc
    23281 atatgactga aatcacccat ccaaggactt ggttgatggg taagacggta gccttgaaaa
    23341 acgcggtcat caggatgaaa ccaccagctg ccgttttgat ccgtagtttg cggtgcaaaa
    23401 taattcatcc caaaaggaac gccagtataa ggctggcagt tcccattaga gaaatttggc
    23461 tgattcgctg ttgcatgtct agtatctatt tgaccgatat tcattttatt tctcctttgt
    23521 tgattgtctt ttgatgaatg aaactggaaa tttactatcg gatggtcgat cttttgtctc
    23581 taaccaagtg accaaagcat ctcccgcaag cttacctaac tgttcaaaat cttgtgcgat
    23641 ggttgtcagt ggcgggtcaa caagagctgc tgcttggata tcatcaaatc cgataattga
    23701 aaaatcttca gggatatgat aaccatgctg tctcaagaga ttcatggtct ggatagcgac
    23761 aacatcgttt tcacaaacaa aagcagttac ttcatactta gtgatataag aaagcaattc
    23821 atctaatgtc gcgcttttgt catccagtga agtgtggaaa gtcaaaccag ctttgtccaa
    23881 tgcttcaagg taaccaaggt aacgctgtcg aacggattgt ggatgagtct gcgtaccaaa
    23941 caaataagcg atttttgaat gtccctgttc gatcaaataa gaggcagcaa gcgcaccgcc
    24001 ttggaaatta tcggaaagaa cggtaggaaa aggtaactca tagatttttt tatctaatat
    24061 gacgacaggg aattctttca atgaaagctc aaaaagtaag tcaagatgtt gttctgtatc
    24121 aagagcataa taaatcaatc cgtcaaattc attgattagc tcttttgctg ttttattttg
    24181 tagaaagtca agcgtcgtca tcatgacatc aatctgttta tcttgcatca cagggttcaa
    24241 tcccttgcta aaatcaccaa cagacaaatc agcgacatat ggaagaagaa acagaatccg
    24301 tcgtgttttt gtaggagaag caagaatctg ttccggagaa gcggatttaa cgaaactgcc
    24361 tttcccgcga acgcgataaa taagtcctaa ttgctctaat tcagtcaatg cacgtttgga
    24421 agtgattctg cttactttat aggtacgtga aagttctttt tcagttggta gttggctgtc
    24481 tacaggtaat tcaccagtta aaatagccgt ttgcaaatca ttaaatattg tctggtacaa
    24541 tggttgagtc atataaacac ctcttaatga tatatcatat tcaaataata ccataatgga
    24601 tgaaaaagta aataaaaaga tattttttga aagcgtagac ataattggct gtatgaagta
    24661 taatctttaa agatatatca aatagaaaga aggttttatt agtggtttat aaagatatac
    24721 caaaatcggt gcagaaattt atggaagata tcacacaaaa atgtggggat atccatgcgg
    24781 actgggcaaa gaattttaca gcagcatttg ccaatactct gttgacaaca gtcaaaaagc
    24841 aaaatgacgg aaccaccttt ttattgacgg gagacattcc agcgatgtgg ttaagagact
    24901 ctactgcaca agttcgtcct tatttagtga ttgcaaaaga agatgaggaa ttagctgaca
    24961 tgatcagcgg gttagtcaaa aagcaatttt tttatatcaa tatcgatcca tatgcgaatg
    25021 ccttcaacga agaagctaac ggtgctggcc accaaacaga tcatacaaaa atgaacgatt
    25081 ggatctggga aagaaaatat gaaattgatt ctttatgcta cccaatacag ttggcctatc
    25141 ttctttacaa aaatacagga aaaactgatc aattcaatca atcttttatt gaaggtatca
    25201 aaaaaatctt ggacgtgttc aaaacagagc aagatcatga acactctcct tatacatttg
    25261 tacgagacac gacaagaaaa gaagatacgc tgatcaatga cgggcgaggt gcaaaaacag
    25321 cttttacagg aatgacatgg tctggtttcc gtcctagcga tgatgcttgc cgttatggat
    25381 atctggtacc atccaatatg tttgcggtgg tcgtgttagg ttatgtggaa gaaatttttc
    25441 aaacgatact cgatgataaa gaaattgtac agcaaacaca gaaattgaag aaagagatcc
    25501 aagaggggat tcagaatcac ggacgaacgg ttaatcaaaa agggcaaacg atttatgcat
    25561 atgaagtaga tggaatggga aacgccacag tcatggatga cagtaatgtg cccaatctga
    25621 tcgccgcacc ttacttggga tattgttctg aacaagagga agattatctg caaacaagaa
    25681 gaacattgtt gagtaaagaa aatccatatt tctatgaagg aaagtacgca aaaggaattg
    25741 gatcctccca tacgccagaa aaatacgttt ggccgattgc attagctatg gaaggaatga
    25801 caacgtcaga caaatctgaa aaagagcgga tattgaattt acttgtagca actgacgcag
    25861 gaacacacct tatgcacgaa ggattcgatg tgaacaatcc tgaaaattat acgagagaat
    25921 ggttttcttg ggcaaatatg atgttttgcg aattagtcat ggattacttt gatattagag
    25981 tagaaaagta ggaggtatcg taaatgaaaa agaaagtata tatcatttct catagccact
    26041 gggatcgtga atggtacatg gcttacgaac agcatcacat gcgactgatt gaattgatag
    26101 acgatctact agagctattt gaagttgatc cgtccttcaa cagttttcat ttggatgggc
    26161 aaacgattat tttagatgac tatcttcaag taaggccaga aaaaagacag gcaatccaac
    26221 aagcaatcaa tgaaggaaaa ttgagaattg gaccatttta tatcttgcaa gatgattttc
    26281 ttatcagctc ggaatcgaac gtgagaaata tgctgattgg catggaagaa agccgtaaat
    26341 ggggaacccc tgtcatgtta ggctattttc ccgatacatt tggaaatatg ggacagacgc
    26401 ctcaattaat gaaacaagct ggtatttcag cagcagcgtt tggccgtggt gtaaaaccaa
    26461 ttggctttga taatcaagtt ttggaagcag aaaactattc ttcccaatac tcagagatgt
    26521 ggtggaaagg ccctgatcaa acagcaatct ttggtctgct atttgccaat tggtatagca
    26581 acgggaatga gattccagta gaaaaagaag cagctcttgc cttttggaag caaaaattag
    26641 cggatgcaga acaatatgcc tctacgaacc acttattgat gatgaatggt gttgatcatc
    26701 aaccggtaca aaaagatata tcaaaagcaa tccatctcgc aaatgaactg tttccagatt
    26761 acgagtttat tcattctaat tttactgact atctggaagc agttcaaaaa gatgtgccag
    26821 aagacttagg ctcagtagaa ggagaattga caagtcaaga aacggatggc tggtatactt
    26881 tagccaatac agcatctgct cgggtttact taaaacaatg gaataccaag gtccagcgac
    26941 agctagaaaa tattacagaa ccacttgcga cgatggctta tgaagttagt ggaaactatc
    27001 cccatgatca attagattat gcttggaaga cattaatgca aaatcatcct cacgacagta
    27061 tttgcggttg ttcagtcgat tctgtccatc gggaaatgat acctcgtttt gaaaaagcag
    27121 atgaagttgg aaagtatctt gcacaagatt ccttagaaca gctgaccgca gcaatcgata
    27181 cgaccggatt cccaaaagac agctttccat ttgtcattgt aaatactgca ggaatggata
    27241 aaactggtga agcagaaata accatcgaat tagaaagaaa aagattcgct gaaggaatac
    27301 ctgaacaact atatcaagag ttagaaaatt tgcctaaacg taagtatcat gtagaaacaa
    27361 aaagtggggc cactatacca gctattttat ctgaagaaac tgtccaattt ggttatgatt
    27421 tgccgaaaga ccgtttccgt gtgccatata tggcaagaat gatcaaagtg actttaccat
    27481 tagaaaacat gccagcattt tcttgggaaa cattcgcgct tgtagaaggg gaagcgaaag
    27541 cagaagaaaa ggaaacaatg attcatcaga gtggacgaat catcgaaaac ggccctctac
    27601 atttgacgat tgaaaaaaat ggtaccatta caatggaaga tcgcaaaaac aaacgaaagt
    27661 tgaataatct gcatattttt gaagatatcg gcgatatagg aaatgagtat atctttaaac
    27721 aacctttctg tgatcagcca atcctatcca gtaacaaaga aaactctgaa gtaaaagtat
    27781 tagtagatac accggaaata gcaaaaataa gtattctaca ggaaatggaa atccctgttt
    27841 ctgcagacga gcgtttagaa aaagaacagc agatggttgt ggaatttcgt tatcgaaaag
    27901 cagaacgatc caaagaaaaa cgaatccttc aaataaaaac aatcatgaca atcagaaaag
    27961 atagtaaaaa aatcgacttt gaaacgactc tcgacaatca gatgaaagat catcgcttac
    28021 gtgtcttgtt tcctacaaag ttacatgtgg aacatcacga agcagatagt atttttgaag
    28081 tggtgaagcg cccaaaccat gtgtctaaaa gttgggaaaa ccccacgaat ccgcaacatc
    28141 agcaagcatt tgtcaatatt catgatgaag agtacggtgt aacagtcgga aactttggtt
    28201 tgaatgaata tgaagtaaca gaagacggac aaatcgcagt gacgctactt cgcagtgtgg
    28261 gagaactagg cgattgggga tattttccaa caccagaagc acagtgtcta ggtgagcatc
    28321 ggttcaatta cagcattgaa ctacatggac cagaagaaaa attctctact tatttgcatg
    28381 catacgcagc acagattcct ttttccactc aacaaatcaa acatcatgaa ggaacattga
    28441 tttcaaaaca acaatattta acgataaaaa gtgaaacgtt tgcaattaca gctttgaaac
    28501 gcagcaagtt tagtgataaa gtagtagtaa gaggtttcaa tatgtctagt catttagaaa
    28561 aattggaaat aactaaagac aatggaaaaa ctgtcatatt gaatctgctt gaagaaccaa
    28621 cgaagcaagc ggttgtccca ataatccaac cttatgagat tcggacaatc ggattcgagg
    28681 aggaaaacga atgtatggcg gaatagaagc aggcggaaca aaatttgtgt gtgcggtttc
    28741 agatggtgga gaaattttag aaaaattaag tatccctacc acaacaccag aagaaacatt
    28801 ggatcaggta tttgaatttt ttgatcgtta tgaattgaag ggaatcggga ttggctcatt
    28861 cggcccgatt ggtatcgaca aaacaaaaga taattacgga tacgttttgg caactcccaa
    28921 aaaagggtgg gcacaattcg attttcttgg cacaatcaag gaacgctaca atattcctgc
    28981 tgcttggaca acggatgtca atgctgctgc atatggggaa ttgaagcaag gagcagcaca
    29041 aggaaaaaat agttgtattt accttaccgt gggaacagga atcggtgcag gtgtcgtgat
    29101 caatgaagaa attttttcag ggatcgcaca tcctgaaatg ggacatattt gggtgaaacg
    29161 ccatccagat gaccattatg aaggtacttg tccttatcat aaagattgtt tagaaggatt
    29221 agcagcagga ccgtctattg aggcacgcac agggatcaaa gggcaagatc tgccgcaaga
    29281 tcatcctgtt tgggctattc aggcttatta tattgctcaa gcactgatca actatacgtt
    29341 gacgttagca cctgaaaaga ttattttagg cggtggggta atgaatcagg atcatctcct
    29401 tcaaaaaatc cgtcagcaat ttgttgaatt gatgggtggt tatatggaaa cacctcaagt
    29461 aagtgaatat atcgttcgtt ggggactgcc gaatgaaagt gggattatcg gtagtttgct
    29521 gttagcagaa cacgaacatc aaaagtaagc agacaaatta cacaaaaaga acagagtctt
    29581 atcattatct gctgataaga ctctgttctt ttttatttct attcccataa tccaggtaaa
    29641 gctttaccga ctattttttc ttgagtaaat ggatggatca tgtgcagttc taatgcatgg
    29701 agcatcagtc gttcagctgt tctattttgg tagagagggt ctccaacgat cggatgtccc
    29761 ttatgagcta aatgaactct gatctggtgt gtacgtccgg tttccaaaac acaatagact
    29821 tgtgtttgtt cagaaagtac tttgttgaca gtgacatgtg tcaaggcgct ctgaccattt
    29881 ttatcatcta cgaccctttt tctccgatca tggcgatctc gtccaatttt attggtgatg
    29941 gtcatatctt tggcaatttc tccccatacg atggcttgat atctccgata gatcattttt
    30001 tgttccaaca ttcttccaag tatcggcaag acaaatggat tttttgcaaa taaaacggca
    30061 ccgcttgttt ctttgtccaa gcggtggaca acgtaaggtc gttgctgttt agtgtcgaga
    30121 taagcagcta aatgattgag taaggtactg ttttctgtcg gttcatttgg atgagtcttc
    30181 attcctgcag gcttatccac tataatcaaa tgctcatctt catataaaac acggatgtgc
    30241 ttcgcatctc ctaattgaac ttcttgataa ttataatctg tttcttcgaa taacaatgtg
    30301 atctggtcat tgccctttac ttcatgatgg aacaaagcag gttgttgatt gatccaaacg
    30361 tttttacgta tacgcaaaaa gtgtcgaacc tttctaggaa ctaaccattc cttttcaagc
    30421 aagtcgcgaa ttgttgtttc aggatggttc ataggtaaag tgatcgtgat tttcacaagt
    30481 gtactccttt cagtgcaatt gcagactaat tgtaccattt ttaaaacata tttaagaaaa
    30541 aacttacatt tttctgttca ttcttatgtt actctacttg cactaacgta aaaaaacata
    30601 ttgaactaca acttaagcag gaagaaaaca ctctttcact atggtaaaat aagacgattg
    30661 aatcaaatag acaggagaag aagcaaatgg attttcaaag cattttagaa aagataaaag
    30721 ctgctttgat cagtttttgg cgttggatca gaccatatct cggacaattc catcgatggc
    30781 gaaagcgcat ctggaaaaaa tatcatgtga acaaattgat tttgctgatt ggtttgatct
    30841 gtgtcttagt gacaagtatc tacctcttta ttttagcaaa acaagcaaat gtcgaaacgt
    30901 taaaatcagg attaagccaa tcgacagtca tttatgataa gaaaaacgaa gaagctggca
    30961 cactgtacgc ccaaaaagga acatatgtag aattaaacgc gatttctccc tatatccaag
    31021 atgcagtaat ctcgacagaa gataggaatt tttacgagca tcacgggttt gatatcaaag
    31081 ggattgcccg tgcagcagtc cgaatggtct tgtctggcgg aacatcaggg ggcggaggaa
    31141 gtacgatcac tcagcagctt gccaaaaatg cgtatctgac attagaccaa acgtttgatc
    31201 gaaaagcgaa ggaattattt ttggcaatcg agatcgaaaa gaagtacagc aaagaagaaa
    31261 tcttgaccat gtatcttaac aatgcctatt ttggcaacgg ggtatggggt gtccaagacg
    31321 cttccagaaa gtattttgga gtagatgcaa cgaatgttac gctgtctgag gctgctactt
    31381 tggcaggaat gctgaaaggt cctggtattt ataatccgat cgatcaccca aaaaatgcga
    31441 atgatcggaa aaacacagta ctaagtgtaa tggaagaaaa cggaaaaatc acaaaagaac
    31501 aagaacaagc agaaagccag acagatatca gcgcgtatct taatgacaca tacgataatt
    31561 cagattctgg ttatcgttat ccttattatt ttgatgcagt gatcgatgaa gcagttaacg
    31621 agtatggtct tgatgaagaa gatgtcatga ataaaggcta taaaatctat acagctttag
    31681 atcaggatta tcaaaagcaa ttagaagcta cttatcaaaa cagtgcatta ttcccagcaa
    31741 atgcttcaga tggtgccatg gtccaatctg cttcagtagc tttagatccg aatacaggtg
    31801 gtgtacaagc actagtagga cgaagaggag atcatgtctt tagaggattc agctttgcta
    31861 cacagatgcg tcgttcacca ggatcaacga tcaaaccatt aagcgtatat acaccagcac
    31921 tggaagctgg atacaagcct tccagtatct tgaaagacga accgcaaagt tattacgatg
    31981 cgcataattt tgacgggaca taccaaggag aagtaccgat gtatcaagct gttgcacaaa
    32041 gtttgaattt gcctgctgta tggcttttgc atgagatcgg tttacaaaaa ggatatgata
    32101 aagcagaaga attcggcctg cctttagcaa aatctgataa gtattatggc ttagcccttg
    32161 gtggactgga aaaaggagta tctccattga tcatggcggg tgcttacagt gcatttgcca
    32221 atgatggtca gatgtatacg ccgcatctta tcacgaaaat cgttgattcg actggagcgg
    32281 tgatcgtcga taacacaaac aaaaaaccaa aacaggtcat atcaaaggaa acagccgatg
    32341 aaatgaccag catgcttctt ggtacatttt caaatggaac agcggctgct gcgaatcctg
    32401 caggttatac gatagcaggg aaaacaggaa ccacagaaac taatttcgat gccacaaaag
    32461 caaatgacca atggatgatc ggttacacgc cagacgtagt gatttcgaca tggatgggtt
    32521 acgaagaatc cagcaagctg cactatttgg aagggacaag tggaaatgtt gtcgggaaag
    32581 tcttcaaatc agcggcagaa ggcatccttc cgtatacttc aaaaacagga tttagtgtag
    32641 cagatgccta tgccacagga ggacaagtgg tagctgcaga tcaagttgga aacacaggaa
    32701 attctaatgg agagtctgga aattggcaag ataacttgaa tcaatatggt gaacaagcaa
    32761 aagaagggct gaaaaacttc ggcgatatgg tgaaagaagg cgttcaggga attggtgaag
    32821 cggctaaaga cctctggcgg aaatatcaag gagagtagcc cctttgaaaa cgaataaccc
    32881 catgttacaa tagagagtaa ctactaaaag aaaagagggc ataacatgtc aaacatctac
    32941 gatagcgcga atcaaatcga acgtgagatc cgtgaattac cagaattcaa agcgttgaaa
    33001 gaagcgtacg agaaagtaca agcaaacgaa gaagcacaca aactgttcaa agacttccaa
    33061 gcgatgcaaa tcgaattaca agaaaaacaa atgagtggac aagagttcag cgacgaagat
    33121 gcaatgaaag cacaagaaat ggctgtgaaa atccaatcag aagaagtaat caatgacttg
    33181 atgcaaaaag agcaaggctt cagtacaatc atcaatgact tgaaccgtat catcatgaca
    33241 ccagttcgcg acctttacag cgaataaaac tattttatga aacaacagag ggtgtgaaag
    33301 agacgttcgg ccttgagcga tgacaaaatt gcttcttatt gccgataggc tgtctcttga
    33361 acaccattta ccaagaggtc gtgagcctac cgtttaactc tgagttacaa ggaacaattt
    33421 caaaaaacag cttctcatgt ttgatgacta ttgtgacttg tgaccgagga gttggcagga
    33481 gaacaccgtt tattcagaaa taggagctac gacaaaagtc ttgtcatagc tcctattttt
    33541 tgtcattcat ttttcaagcg gtagaaatag cctttttgag ctaacagttt ttcgtaagta
    33601 cctatttcaa cgatttttcc ttcatcgatc acaacgattt tatctgcctc tttgatcgtt
    33661 gaaagccgat gagccacaat aaaagtagtg ggtgtttctg tcaggtaatt cagggcttgc
    33721 tggatttttt tctctgactg gttatctaaa gctgaggttg cttcatctag caaaatgatt
    33781 tttggctgac gtatcaacgc tcgtgcaatg gagatacatt gcttttggcc accagacagc
    33841 ttgttgccat gttcgcctac taaggtatcc agaccatcag gaagctggtc aatcagtgaa
    33901 gaaagttgcg cagcttcgat gacttcatct aaacgttctt ttgatacgtt tttcaagcca
    33961 taagtgatgt tctctttgat cgaagcggag aacaacaaag tggtttgagg aacaacggct
    34021 aaataatttc gcacacttcg catatccagt tcatccaaag gctgatcatc tagaaaaatt
    34081 tttcctgaag tcggctggat aaatccaatc aataagttaa ggagtgtaga ttttccagag
    34141 ccagatggcc caacaaaagc aattgtttct ccagctttca cctctagaga aaaatgatcg
    34201 ataatcggtt tgagtgagtc cggataaaga aaagaaacat cctcaaaacg aaaatttcca
    34261 tccaaatgtt ggattttttt cttaccgtga taagtttcta catcgccagc aatcaacagt
    34321 tcgccaatag aagtgataga ttccatgcct tttgaaatcg taggaatcag cgtgatcatt
    34381 ccagaaacgg aattgacgat cgtcgtgaaa tacgtctgat acatcacgac atcgccgggt
    34441 tggatgcgcc cttgtaaagc taatatccca gtgaaaatca aacaaatcag ctgaaatatc
    34501 tgaaaactag cccagctgac tgatccgaaa acagattgca ggatatctaa gtggaatcct
    34561 ttttcataga tttgagtaaa atgcttattt aagcgagttg tttcgtgttt ttctaaccca
    34621 tgagctcgag caatcggtgc taattcgatc acttcgatca cttttgcaga agtctcttcc
    34681 atttcttgcc tgaagtctga gttggactgt ctgatccttt tacggaaaaa gagagtaacg
    34741 aatgcggcga gaggaacagt caataagaaa aacatgaaaa caacaggact tttataaagc
    34801 gtgataccta aagctacgaa taaattgaca gcgatattta atccgctgac aaatacttga
    34861 gaggacaacg tttggatagc ttctacatca cggatgatct ttgactggat tcgtccagat
    34921 tgcaattctt tttgataagg aatagatagt tcttggattt tcttgataag ggtcgctctt
    34981 agtccagctt ctacttgacg gatcgaagtg ctatggtatt ttacatgaaa ataattcgtc
    35041 aatatatttt gaccaatcag acaagccatc aaaaaagcgt tgaaataaat ggcctgcaac
    35101 tgagaagaat ctttcgatga agctgcattg attacattgg caatagcgat cggcattaac
    35161 caagatggaa gatgtttgat aataaaaaag agtcctgaca tggcaaaacg ccaataattt
    35221 ccctgataaa gatgcagcaa tgtctgaaaa gcatttgcat gatatttttg ataacttttc
    35281 tgtattcgtt cctccataat cttctcctta acaatcttgg taaataaact gcaagtgttc
    35341 tattgcttca ggaacataaa tctcaggagt agcttcaaga gtggcgtaca ttagaggacg
    35401 ctcatatttg atataatgca gtaccgggcg gaaatctaaa cagcccttgc caacaggtac
    35461 catgcggatc tttccatttt ccatgatata gtctttgaga tgaagcgctg taatataacc
    35521 atgtaaattg tccaaggcac ctcggatcac tttttcctga tcagcatgat tttctttatg
    35581 catcaaattc gcacaatcta aaatgatttt taaatttggt gaagctactt catcgattaa
    35641 acgtttagct aactgatagt tatagattgg atgattgatc cctgcttcaa cagcaacagt
    35701 tacgccaaaa ttttctgcat gagcgactaa ttcaatgata gaatttctgg cgatctgata
    35761 agcttcttca gtaaaatttt cttttgtgta accgacagag acactaccgg tttccgtagc
    35821 aatcatcttg gcttgatagg cagagcacag agcaatttgc tgtttgaaag ttgccaatgc
    35881 ttgtgcacga acttcttgat ttttgctgga taggttgata tagcatccta aaatggaaag
    35941 ttcgatattg tttttttgga agtattcacc aaaataggca gcactcccaa gtgtcatgtc
    36001 agttaattta agttgttcag gaaatgcttt ttgtggtgca aattggatat gggacaagtg
    36061 aagctgtgat actttttgaa cgatttcttc acgatttcga acatctaaat cgtgggcacg
    36121 gatgcctaaa tttaatggaa acatctcaaa ctcctttttg taaataatta gcaagaggca
    36181 gacgggattc ttttatcaat tcaacaatta ttcgggcaac tttatcagcg ccttctggac
    36241 taaagtgggt attgtctttt agtccttctg ggtaattttt acaagtatca ggcataagat
    36301 gcaggtagaa tttttttgtc tcttcttttg agaaggtctg gaacaattct tgtgatcttg
    36361 agaacacatc taaaagagga atatggtgtt tctcagcaaa tgcacgcatc gcttttggat
    36421 agtctccaag tatatctgga ttcaatgttc cgttttctaa gtaatctcgt cgcgtcacag
    36481 atgtcagtaa gaccatttgg acgttcgctt tttttgcgca ttcaaaaaat atctgcagat
    36541 tttttaaata gctaccgaag ggaagcgtac ctctttcttc ttggattttc tggtcgttat
    36601 gtccaaattg tacaaataag aaatcattcg gctgaaactg tgaagctaga cgggctaacc
    36661 ttccttcctg gataaatgat ttggtgctgc gtccattctt tgcttggtta ttgatctgga
    36721 tctcgggtga aaaataagca gagatttttt ctccccaacc tgtctcggga cgcttgtttt
    36781 catctttttc tgccgcagta gaatcaccgg caatatggat agtaggcatg ttatctcttc
    36841 tttcataaag gtttataggc aatgaagcag aacagcaaga gaagaagacg gagcaa
//
LOCUS       DARBFO010000016        35398 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000016 DARBFO010000000
VERSION     DARBFO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.017"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            8..649
                     /locus_tag="SCK51_000973"
     CDS             8..649
                     /locus_tag="SCK51_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251615.1"
                     /translation="MIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLSAV
                     LHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMRYQ
                     IDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIH
                     RFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="SCK51_000974"
     CDS             708..1148
                     /locus_tag="SCK51_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251616.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="SCK51_000975"
     CDS             1160..1828
                     /locus_tag="SCK51_000975"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEG1251617.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="SCK51_000976"
     CDS             1892..2515
                     /locus_tag="SCK51_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEG1251618.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="SCK51_000977"
     CDS             2571..2957
                     /locus_tag="SCK51_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEG1251619.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="SCK51_000978"
     CDS             3170..4561
                     /locus_tag="SCK51_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEG1251620.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5172..5540
                     /locus_tag="SCK51_000979"
     CDS             5172..5540
                     /locus_tag="SCK51_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEG1251621.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5720..6661
                     /locus_tag="SCK51_000980"
     CDS             5720..6661
                     /locus_tag="SCK51_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEG1251622.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6730..7461
                     /locus_tag="SCK51_000981"
     CDS             6730..7461
                     /locus_tag="SCK51_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEG1251623.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7605..9785
                     /locus_tag="SCK51_000982"
     CDS             7605..9785
                     /locus_tag="SCK51_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEG1251624.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9805..10287
                     /locus_tag="SCK51_000983"
     CDS             9805..10287
                     /locus_tag="SCK51_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEG1251625.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10303..11064
                     /locus_tag="SCK51_000984"
     CDS             10303..11064
                     /locus_tag="SCK51_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEG1251626.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11195..12529
                     /locus_tag="SCK51_000985"
     CDS             11195..12529
                     /locus_tag="SCK51_000985"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEG1251627.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12836..13321
                     /locus_tag="SCK51_000986"
     CDS             12836..13321
                     /locus_tag="SCK51_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEG1251628.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13314..13997
                     /locus_tag="SCK51_000987"
     CDS             13314..13997
                     /locus_tag="SCK51_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEG1251629.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14123..16768
                     /gene="polA"
                     /locus_tag="SCK51_000988"
     CDS             14123..16768
                     /gene="polA"
                     /locus_tag="SCK51_000988"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEG1251630.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16813..17649
                     /gene="mutM"
                     /locus_tag="SCK51_000989"
     CDS             16813..17649
                     /gene="mutM"
                     /locus_tag="SCK51_000989"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEG1251631.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17613..18242
                     /locus_tag="SCK51_000990"
     CDS             17613..18242
                     /locus_tag="SCK51_000990"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEG1251632.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            18294..18626
                     /locus_tag="SCK51_000991"
     CDS             18294..18626
                     /locus_tag="SCK51_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEG1251633.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18938..19429
                     /gene="nrdR"
                     /locus_tag="SCK51_000992"
     CDS             18938..19429
                     /gene="nrdR"
                     /locus_tag="SCK51_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEG1251634.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19452..20825
                     /locus_tag="SCK51_000993"
     CDS             19452..20825
                     /locus_tag="SCK51_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEG1251635.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20825..21751
                     /gene="dnaI"
                     /locus_tag="SCK51_000994"
     CDS             20825..21751
                     /gene="dnaI"
                     /locus_tag="SCK51_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEG1251636.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21880..22320
                     /locus_tag="SCK51_000995"
     CDS             21880..22320
                     /locus_tag="SCK51_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEG1251637.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22464..23165
                     /locus_tag="SCK51_000996"
     CDS             22464..23165
                     /locus_tag="SCK51_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEG1251638.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23162..24283
                     /locus_tag="SCK51_000997"
     CDS             23162..24283
                     /locus_tag="SCK51_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEG1251639.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24298..25530
                     /gene="pepT"
                     /locus_tag="SCK51_000998"
     CDS             24298..25530
                     /gene="pepT"
                     /locus_tag="SCK51_000998"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEG1251640.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25662..26570
                     /locus_tag="SCK51_000999"
     CDS             25662..26570
                     /locus_tag="SCK51_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEG1251641.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26605..26877
                     /locus_tag="SCK51_001000"
     CDS             26605..26877
                     /locus_tag="SCK51_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEG1251642.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26888..27934
                     /locus_tag="SCK51_001001"
     CDS             26888..27934
                     /locus_tag="SCK51_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEG1251643.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28185..30794
                     /gene="clpB"
                     /locus_tag="SCK51_001002"
     CDS             28185..30794
                     /gene="clpB"
                     /locus_tag="SCK51_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEG1251644.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30945..31631)
                     /locus_tag="SCK51_001003"
     CDS             complement(30945..31631)
                     /locus_tag="SCK51_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251645.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31609..32103)
                     /locus_tag="SCK51_001004"
     CDS             complement(31609..32103)
                     /locus_tag="SCK51_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEG1251646.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(32123..33343)
                     /locus_tag="SCK51_001005"
     CDS             complement(32123..33343)
                     /locus_tag="SCK51_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1251647.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33490..35325
                     /gene="lepA"
                     /locus_tag="SCK51_001006"
     CDS             33490..35325
                     /gene="lepA"
                     /locus_tag="SCK51_001006"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEG1251648.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        11847 a         6250 c         7612 g         9689 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     4801 caccgtttaa gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt
     4861 caaaaaatag cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga
     4921 gaacaccgtt taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt
     4981 ccatataaat acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa
     5041 cttatttatt attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta
     5101 gattttccca ttttcctatg gtaaactgac aaagttgaga atgttagaac agatcgttta
     5161 ggaggaaaca gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa
     5221 aaaatgcaac gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg
     5281 ggacgcttat aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc
     5341 gagcatttgt tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt
     5401 acagtaaaga tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg
     5461 atatggtaca gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact
     5521 ttgcggttac aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag
     5581 cgaagtcttt ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt
     5641 atacacaagc aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa
     5701 agaaggaagg aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat
     5761 cgaagaacca gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa
     5821 aggtcgtatt ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa
     5881 cacgactgcc tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa
     5941 gatcgatcca agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct
     6001 tgtaagttta aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc
     6061 accgcaagaa tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa
     6121 tgattatgaa tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt
     6181 tcgagagctt ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt
     6241 gacctactgt actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg
     6301 attcaaagat gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt
     6361 ccaaggagaa ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat
     6421 caaccaagta gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg
     6481 atatatcaat tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga
     6541 agaaaaacat ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt
     6601 tcaagaccat ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata
     6661 gaaaacaata gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg
     6721 gtgatccaca tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt
     6781 tttgaaaaac ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat
     6841 ctcaatgatc gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa
     6901 gaaacaggag ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa
     6961 tcaccggagg actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc
     7021 ctgattcacc caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa
     7081 tcgagatatt cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa
     7141 aattatcttt ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt
     7201 ctaagagact ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag
     7261 acaattgtat ttggacatac gattacacca atgctccatg gagatatgca gacaacagac
     7321 ctctggattc aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg
     7381 catggggttg tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa
     7441 ggaccttggc atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc
     7501 tctttttatt tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta
     7561 tgatagaata aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga
     7621 accagacgat cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga
     7681 ctactgtact taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa
     7741 aagaaatgac tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct
     7801 atcttgaaaa cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga
     7861 aactgactcc cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag
     7921 atttgtatcg tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag
     7981 gacttgaacc actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag
     8041 cagaaaaata tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg
     8101 ctcatgagat tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt
     8161 cttatacgta taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa
     8221 aaggcgtata tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc
     8281 gaatattagc tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag
     8341 atgaagcagc aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt
     8401 cttcttcatt cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa
     8461 tcgagcgaga actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt
     8521 tcggcgaaaa cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg
     8581 gatttgatcc ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag
     8641 ttttagcaat cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg
     8701 ctggaccagc ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa
     8761 atggtacagc cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc
     8821 ataaaaccta ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa
     8881 ttgcacgaaa agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc
     8941 gtcgcctgca agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag
     9001 gccagtacca acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg
     9061 aaacagcggt taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc
     9121 atatatcagg attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg
     9181 gagaattcac cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg
     9241 aacaagcaat cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta
     9301 tccatcccga aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa
     9361 aagagcttgg tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg
     9421 ctgaaagcct atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac
     9481 ctggacgaga tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta
     9541 tggaagactt gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg
     9601 gcgcatttgt tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca
     9661 aaaaattcgt caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga
     9721 tagaacaagt cgatacgaaa aaaggcagaa tcagtttgac gatgctttcc ccttatgaag
     9781 agtaacaatg aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag
     9841 atatcattaa aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta
     9901 cgtacaactg gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta
     9961 tttgaagagt cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat
    10021 catcttcatc tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg
    10081 aaaaaaacag gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag
    10141 tattatcgat gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc
    10201 aaatatcgtt gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac
    10261 tttaatgaag cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag
    10321 aggaatctaa aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat
    10381 tttgaggaac tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct
    10441 gataaagcag cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg
    10501 gtggggcatg cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa
    10561 gatggcatga gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc
    10621 gataaggctt ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca
    10681 ctggagattc ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg
    10741 acactgcaac gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt
    10801 ttcgcgaatg ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt
    10861 acagcaaaag aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga
    10921 gaaatggaac gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt
    10981 cctcacattg atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata
    11041 aaaacggatt tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat
    11101 ttggttgaaa ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa
    11161 aagaatggac gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca
    11221 ttttgttggt atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg
    11281 tctaaatgtc caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa
    11341 agcaaatatc acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat
    11401 tgcaggaaat gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt
    11461 ggaagtgatt cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc
    11521 tgtaacaggc tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg
    11581 tgtccgtcca acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga
    11641 attcttttca tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta
    11701 tgcaatcatg accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt
    11761 atttacagct ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga
    11821 cgatgcttat ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga
    11881 aaacgatgat atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt
    11941 ctatcatggg gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat
    12001 cttaaatgct ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt
    12061 tgcagaagaa atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc
    12121 agacatgacc gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga
    12181 tggcgcacgc caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt
    12241 tacgcgtacg attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt
    12301 ttacttatgc gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga
    12361 ccttggagcg aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt
    12421 gttagattat catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga
    12481 acaagcgtat gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg
    12541 cagtgtcctt ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt
    12601 ttgcaactta tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa
    12661 tcaaaaacat gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt
    12721 tttcacgacc tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt
    12781 acgctacttt tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa
    12841 cataggtaag ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt
    12901 atccttaaca gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga
    12961 tgcaaatcct ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat
    13021 cgttccatca gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg
    13081 accaggatca catgttgtca atttttctgt caattttatt gaaccggttt tccattatga
    13141 aacactgact tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat
    13201 ttctgtagaa gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca
    13261 accgccacaa gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata
    13321 atccaagtac atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag
    13381 gtatgggact tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc
    13441 aattagcaac ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac
    13501 tgattttagt aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg
    13561 gatttattgc ctactctctt ttgaatggca taatattagc ggttactctt gcatactacg
    13621 atattggaac agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg
    13681 ttgtcggagt aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt
    13741 taatcggagt gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg
    13801 agctgttcat ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc
    13861 aaagcatccg taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat
    13921 tcatggcatt gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct
    13981 ttggacggaa caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg
    14041 gtatggtccc gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac
    14101 tttacagtaa ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag
    14161 tgtggctttt cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa
    14221 cggtcttcat acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa
    14281 agagcaaccg actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga
    14341 aatgtatgcg gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat
    14401 gccatatata cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta
    14461 tgaagcagat gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt
    14521 cgtcgtgctt tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga
    14581 tatcactgta aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa
    14641 atatgatggg ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc
    14701 tgacaatatc cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca
    14761 atatggctca gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa
    14821 agaaaatctg ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa
    14881 tacttcagcg cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga
    14941 taaactcgtt ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat
    15001 cgtagaagaa gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga
    15061 ggaaatgttt actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac
    15121 agaggaaatc gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct
    15181 atctgtattc gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa
    15241 agtatatgat gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg
    15301 aatcgatttt gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga
    15361 tatcgaagga atagctgctc attacggata caacgatatc caatcagatg aagctgtata
    15421 cggcaaaggg gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc
    15481 gcgaaaagta gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagaaaa
    15541 aaaccaagaa gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat
    15601 ggaaataagc gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc
    15661 tgctcgctta agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt
    15721 gaattcacca aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa
    15781 aaaaacaaaa actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc
    15841 accaatcgta gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt
    15901 cgagggactg ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac
    15961 attgacccaa acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg
    16021 attagatgaa gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat
    16081 ttattcttct gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga
    16141 acatttgaaa gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt
    16201 atttggaatc gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt
    16261 gaattttggt atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag
    16321 ccgaaaagca gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga
    16381 atatatggaa agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca
    16441 ccgccgtcga tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga
    16501 acgaacagct atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat
    16561 gatcgaattg gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt
    16621 tcatgatgaa cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt
    16681 gaaggaagtt atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg
    16741 gggaaaaacg tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg
    16801 tgaaaagaaa aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg
    16861 ttagtagtag gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa
    16921 ccagagacac ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt
    16981 agagggaagt ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg
    17041 gaaggaaaat atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt
    17101 ttcttttttg aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg
    17161 acaatcgtcg aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag
    17221 cctttgccag atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa
    17281 gcaatcaagc cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta
    17341 gatgaagcat tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc
    17401 aaagaggtag aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc
    17461 ggaggtacca cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta
    17521 gcactgcatg tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg
    17581 aaaacaaaag tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc
    17641 gcaaagtagg atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag
    17701 ccaaagtctt tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa
    17761 ctgttgagcc tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat
    17821 tgcagcctga tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg
    17881 aaaaacggca aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc
    17941 aaatcgcctc tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg
    18001 aaggtcatta tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc
    18061 agcttcagcg tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta
    18121 gtagtcaact tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg
    18181 gaacaaaaga aatacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt
    18241 gagtatctgt gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat
    18301 cattaaaaaa tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc
    18361 tcaataaaga aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag
    18421 aaaagcagta ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga
    18481 agcggcaaga tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc
    18541 tcccagaaag tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact
    18601 atacaggaat cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga
    18661 tgagaaggtg ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt
    18721 aagttatttc tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag
    18781 gggacacaat tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag
    18841 gacagtcgcg tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca
    18901 tcctttatat aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca
    18961 taataattca cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg
    19021 tcgcgaatgt gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc
    19081 gttattagta atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag
    19141 aggactcatc cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt
    19201 caaccatgtc gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat
    19261 tggtgaatat gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc
    19321 aagtgtttat cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt
    19381 ggataaagca aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag
    19441 gaggcatttc cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa
    19501 gaaaagcggc tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac
    19561 taatcggcgg agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat
    19621 ctggcaaagg tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc
    19681 cgcaatttta tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta
    19741 aagaagatag tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta
    19801 aagcattttt caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac
    19861 gattccagaa gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa
    19921 aaatcacaaa aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc
    19981 aagaaaacat cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg
    20041 aagcagattc gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg
    20101 caagtcttga tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg
    20161 atgaattgac acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat
    20221 ctgaaaaaga actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa
    20281 aagaaacagt tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa
    20341 acgcagcgct tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag
    20401 caatcaagtc agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc
    20461 ttgtttctca atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag
    20521 tgatcaagaa taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg
    20581 cacaaaagaa aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc
    20641 aagcaaaaca atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc
    20701 gacgggaaaa gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg
    20761 agaaaaaagc agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata
    20821 tctagtggaa gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga
    20881 aaagtatgct gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag
    20941 cgcacaccaa gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga
    21001 attcgtgcaa gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta
    21061 tgagccgaag ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc
    21121 attgttagca cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc
    21181 aaaagatatt caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc
    21241 gagcttagaa gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa
    21301 aggactgtat ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc
    21361 gagagaatta gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt
    21421 tgagatgaaa caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg
    21481 ttcacctatt ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga
    21541 cgaagtattc ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc
    21601 atcgaatttt acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga
    21661 agagccgcta aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga
    21721 aatgactggt cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac
    21781 ctagtagtaa aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac
    21841 cgcaacaagt aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt
    21901 atatctgtag ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca
    21961 ggttctgttg attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag
    22021 agacaaagca tatttgtcgg aacgattgaa agtatcactc aagtagaagc agaagaagaa
    22081 acgcttcagt tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg
    22141 tcattcagca atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata
    22201 aatgaattga aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg
    22261 atgtctatcc caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa
    22321 ccaataacat tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt
    22381 ttaaggagat atgggaattt ttttattcct agtctcctta ttttatttgg aaaatgctat
    22441 aattatctag aaggagtgaa gctatgaacc atttagattt atctcagcgc ttagccgcag
    22501 tgggtgactt tgtccctgaa tccgctcgtt tagcagacat tggatcagat cacgcttact
    22561 tacccgttgc attgatatta agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc
    22621 aaggtcctta tgaatcagca cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca
    22681 ccgtacgcct tgcaaatggt ctagatgcta tccagccaca agatggtatt acagctgtca
    22741 cgattgcggg tatgggaggt tcactgatcc gagatatcct agaagctggc aagttgaatc
    22801 aacgcttaag cggacaagaa cgtttgatcc ttcagccaaa catcggagag cgtacattac
    22861 gatcttggct gcaagaaaat cagtataaaa tccaatcaga aaaaatactt gaagaaaata
    22921 aaaaaatata tgaaatcatt gtagccgaaa aagaacaaaa accagttact tacaatgaaa
    22981 tggagctgat gttcgggcca tttctgcttg aagagaaaag tgagatcttc aaatcaaaat
    23041 ggacaagaga gcttcaacaa agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc
    23101 aagaggcacg tcaaaaacaa atcgagcagg aaatccaaat gattgaggag gttttaaagc
    23161 catgacaatc agcggacgca ctttcatcca aaagtttgaa agctattgtc ctcagtggct
    23221 agctgaagaa ggagacccag tcggactgca tatcggaaca ttggataagc caatccaacg
    23281 agtaatgatg acattagacg ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat
    23341 cgatttgctg attgctaaac atcctccaat ctttcgtcct gtaaaacggc tggttactga
    23401 tcagccacaa gaaaaaatgt atgcagattt gttgaaacat gatattgccg tctatgcagc
    23461 acacacgaac atggatatta tttgggatgg actgaatgac tggttttgtg aacttctcgg
    23521 tattgaagtg gagagttatt tagtaaagac tcatgagatt cattataaaa aactggccgt
    23581 ttatgtacct gtggatcatg cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg
    23641 tacacaaggt gattataccg gtacaagttt tacctcgatt ggacacggtc gatttactcc
    23701 ggaagctggt gcacaaccag cgattgggaa agtagggaag acagaacaag ttcaagaggc
    23761 aaaagttgag gttattttgc cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc
    23821 agctcatcct tatgaggaac ctgcttatga tcttttcgct atagatgaac cagtcgaaat
    23881 gtttgggtta ggacgagtag gtgaattgcc gcaagaaatt tctatagagg catttgtaga
    23941 acaagtaaaa gaagcgttcc aactagatgg actgagaata gtccagccaa aaaatgcaaa
    24001 atcctctgtg aagcgcattg cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc
    24061 aatcgcccaa agagcagatg tctatatcac gggagatatc tattatcata cggctcatga
    24121 tatgcaaagc gctgggttga ttgcaattga tcccggtcat tatattgaat cgttatgcaa
    24181 gcaacgattt atcgagaaat ttgaatcttg gaaacaagaa gaaaattggg atattaactt
    24241 tttcgtttct gaaacaaata caaacccatt tcaatttaat taaaaggaga gaatactatg
    24301 tacgaaaatc ttttgccacg ttttttacga tatgtcaaaa cagaaacacg ctccgatgca
    24361 acaagcaaaa cgactccatc gactcagaca caagttgctt ttgcgcaaga attgaaaaaa
    24421 gaattagaag agttaggctt agctgatgtt ttctataatg aagataacgg attcgttatg
    24481 ggaacattac caagcaatgt tgatcatgaa gtgcgttcca ttggatttat ttcccacatg
    24541 gatacagcag attttaatgc agtaggagtc aatccacaaa tcattgaaaa ctatgatggc
    24601 gagtctacga tcgtactaga tgcggaagaa cgctatacat tgaatacaaa agattttcca
    24661 aatttgaaaa attatgaagg ccacacattg atcacaacag atggcagtac attactggga
    24721 gcagatgaca aatcagggat tgcggaaatc atgactgcaa tggagatctt gatcaataat
    24781 ccttctatcc ctcacggtac catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc
    24841 ggagcggata aattcgatgt tgagcaattc gatgtagatt ttgcttatac agtcgatggt
    24901 ggtcctttag gtgagttgca gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc
    24961 caagggaaaa atgtacatcc agggacggca aaagatacaa tgatcaatgc tctacagtta
    25021 gcaatcgatt ttcataatca gcttccagcc gatgaagttc cagaaaaaac ggaaggatac
    25081 gaaggtttct tccatttgat ggcattggat gggaatcctg aagaagcttc tatgagttat
    25141 atcattcgcg atcatgatcg tgaaaaattc gaagcacgca aagcacaaat cacacatatc
    25201 caagaagagt tgaatcagcg atttgatcaa gaacgaatca aagtagatat gtatgatcaa
    25261 tattacaaca tgaaagaaat cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa
    25321 gcaatgatcg agcttggtat ctctccagtg atcgaaccag ttcgtggagg aacagatggt
    25381 tcaaaaatct cttacatggg tattccgacg ccaaatctat ttgccggagg agaaaatatg
    25441 cacggacgat tcgaatttgt ttctttacaa gtaatggaaa aagcaacaga cgtgattgta
    25501 aaaattgcag aattgaacac aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg
    25561 ttcgtcccaa tgatggaatg tcatctgtct aaggctaaag ggcaggacaa tggaattggt
    25621 tgtcccgccc tcttttttga agagtaaata ggaggtaaag aatggaaaag ctgctagttt
    25681 tttataacaa aagttcaggg aaagatgagg gtgagaaatt agcacgctgg tttcaagatt
    25741 atgtaaaaga aaagcagcca gaattatatg tagaactagc tgaaactggc ccatcgattg
    25801 atgatgcgga attagtcaag aaagcagaag ccatagaagc tgatacttta gtaattatag
    25861 gtggagacgg cacgattcat catatcgtcc aagcgttcca aaaaacatta tctcaatacc
    25921 atgtcggatt acttccaggt ggaactgtca acaaccttgc acgtgttctt gagattcccc
    25981 tcaaaaaaga agaagctgct gacgtgatcc tggaaggcaa tgtacggaaa atagactatg
    26041 gtcaagtcaa tgatgaagtc attatctcta cactgacaat cggaatctta gcagatacag
    26101 cagtttggat cagtcaagaa gaaaaacagc gttacggctc gtggatcttt atcaagaagt
    26161 tttttaagtt attgctgaag aagaaaaaat atcatttgga tttagaaaca gacgaggagc
    26221 attggaaagg aaaatcccaa ctgttgacag tcacgatgtc taactccgtg ggcggtttta
    26281 caaattttga cgactcagcg accctagatg atggaaaatt tcatgtaacg atcgtacctt
    26341 ccttagatat tttccgattt attttttatt tgcctagaat catgagaggc aaattctata
    26401 cgatccccgg cattacttat ttcacagcca aacgcatgaa aatccaagcg caggaaaaat
    26461 ctgtgggcac tcgaacggat ggagacacga cagatgatct tccagttgaa ttatctgttg
    26521 tatcaaaagg attaacagtt tattcaccaa agaaagaaga aaaagcttag aaatagtagt
    26581 taacagaaag ttggaagaag agtaatggat gtattgttac acaatccttt gtggttttac
    26641 cttttcattg tgaattttta cttattttgt ctgatggggt atgataaaca tcaggctaga
    26701 actagcggct ggcgtgttcc tgagaataat ttgatatttg tcagcctgtt aggcggtggt
    26761 ttgggaggat tgatctctcg aaaagttttc catcataaaa ctcggaaaaa gaagtttact
    26821 ataggattcg ctgcaggtgt acttgttgat ttgattctca tttatttttt ccattaagga
    26881 gcaggagatg tcgtctaaag gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat
    26941 tgtgattttt gccattattt attatttaat caatcaatca tttgcagata tctttgccga
    27001 attgctgtct accagtgttg ttgttttggc agcggctgtt atattaggga cagtttacca
    27061 aatcgctgaa gggcgctcaa tcaaagaaat tgctgaacct ttcgcgccga attttacaac
    27121 gaaagatggg ttcttgacct ctagctatat tgcgttttac cgaatcgttt cgtttggtac
    27181 aggaacgctg atctcggaga tttattttta taacaaaaaa ggaatgaagc tatcccaggg
    27241 agcaggggtg acagcactcc atatgattat gtacaaactg gcagtgatca cttatgcatt
    27301 agctggcttg gtcatccaat tctctctttt ttattctaat ggtccatcaa tgatctggtt
    27361 tatcatcgct ggtatccttt tgacgatcgt gattgttgcc tttttattgg cgatgtccgt
    27421 cagcttgaat gttcaaatat tttttgtcag cttatcgaat aagttattta aaagtgagcg
    27481 tttgcggaaa tgggtggata catgcaatca gcagatctac tctttaaggg aaacggtcca
    27541 aaccattatc caagacagga ctgcgctatt gcggatttat ctatggaata tgttaaaact
    27601 tgtattttgg tatgtacttc cttatttgat tctgattgaa aaccatccta aaatagattt
    27661 tctactgacc atttcgttta tcagctttgc agtagtgctt tcaggggtta ttcctacgcc
    27721 tgctggtatt ggttcctttg aatttgtcta tttgttatta ttcagaccag ttgttggaac
    27781 agttgacgca gtttcttcgc ttttactgta tcgtttcagt tcctttatct tgccatttct
    27841 ctatggattt gtgtatgtga ttgctgaacg gcgaagagtg atcaaagaag aaataataga
    27901 agtaaaaaaa gagaataaag aaaatcaagg gtaggtcttt tataagatct acccttttta
    27961 tcaaatcaaa gaaaaacgtt atggataaga taattttacg tttgtttggc tcttttttta
    28021 tatacataca atcaattttg tttttttaag atttatataa gtcgagaaaa aaagtcaaaa
    28081 aactatgtgt atattttgac caaaattgac caaagcgata taatagtttt tgtaaagatc
    28141 aaagaaataa gtgatcttac ctttaattag gagggtatac ctgtatgaat attgagaaaa
    28201 tgactacgac gcttcaagaa gcgattgctg aagctcaaca gattgcggtc actcgaaaac
    28261 atcaagatat cgacattgcc catgtatgga aaatcttttt acaatctaat cattttggtc
    28321 gaaatttcta tacggatgcc ggattggatg tcgaatcttt tgaacatgaa attgatcgtc
    28381 tactggatga gtatccagtt gtttctggtg gaaacgtcca atatggacaa aacctcagcc
    28441 agaatttctt tcgattattg aatgaagcag accaaatacg tgaaagcttc ggcgacgagt
    28501 ttctttcaac ggaagtcgtt attttagcgt tgatgaaatt aaaaaattat ccgttaactg
    28561 tttatttgaa taaaaacgga ttatctgaaa aagaattgcg taaaaacatc gaagagatga
    28621 gaggaggaga aagagtgacc tcacaaaacc aagaagaaca atacaaagca ttagaaaaat
    28681 acggcgttga tttagtacag caagtcaaaa gcggaaaaat ggatccaatc atcggtcgtg
    28741 atgaagaaat ccgtgatgtc atccgtattc tttcacgaaa aacaaaaaat aatcctgttc
    28801 tgataggaga acccggtgta gggaaaacgg caatcgttga agggcttgct caacgtatcg
    28861 tacgcaaaga tgtgcctgaa aatttaaaag ataaaacgat tttttcatta gatatgggtg
    28921 cattgatcgc tggagcaaaa ttccgtggtg aatttgaaga aagactaaaa gcagtcttga
    28981 aagaagtcaa aaaaagtgat ggacgtatcc tcttgttcat cgatgaaatc cacaatatcg
    29041 taggggcagg aaaaacagaa ggaagtatgg atgccggaaa tctgttgaaa ccaatgcttg
    29101 ctcgcggtga attacatttg atcggtgcca caactttaga tgagtaccgt caatatatgg
    29161 aaaaagacaa agcgttggaa cgtcgctttc aaaaggtatt agtcaaagaa ccaacagtgg
    29221 aagatacgat cagtattctg cgtggattaa aagaacgttt tgagatccac catggtgtta
    29281 acatccatga caacgcatta gtagcagcag ctactttatc tgaccgttat attactgatc
    29341 ggttcttacc ggacaaagca atcgatttga tcgatgaagc cagtgcaacg attcgggtag
    29401 aaatgaactc tatgccaacg gaattagatc aagttactag acgtttgatg cagttggaaa
    29461 tcgaagaagc tgcactgaaa aaagaatcag atgatgcaag taaaaaacgc ttgaaaaatt
    29521 tgcaagaaga attagctgaa cttcgtgaag aagcaaatgc gatgaagatg cagtgggaaa
    29581 cagaaaaaga agaagtcaat tctgtttcag caaaacgtgc tgaaatcgat aaagcaaaac
    29641 acgaattaga agatgcagaa aataactatg atttagaacg cgctgctgta ttgcgtcatg
    29701 gtacgatacc tcaattagaa aaagaattga aggaattaga agctaaagca aaagacagcg
    29761 agattaagat ggttcaagag tccgttaccg aaaatgagat tgctcaagtc gttggcagat
    29821 taacgggtat ccccgtaacg aaacttgtag aaggtgaacg agaaaaacta attaaattga
    29881 atgaaacact tcataaacga gtgattggtc aagatgaagc agttgatgcg gtcagtgacg
    29941 cggtaattcg ttcacgggct ggattgcaag acccaaatcg tcctctaggt tctttccttt
    30001 tcctaggacc aactggtgtc ggaaaaaccg aattagcgaa agcgttagct gagaacttgt
    30061 ttgactcaga agatcatatg gtacggatcg atatgagtga atatatggaa aaacatgctg
    30121 tgtctcgtct agtcggagct cctccaggct atgtaggtta cgaagaaggt ggtcaattaa
    30181 cggaagctgt cagacgaaat ccttatacaa tcgtcttgtt ggatgaaatc gaaaaagcgc
    30241 atcccgatgt cttcaatatc ttgttacaag tcctagatga cggacgcttg actgattcaa
    30301 aagggcgagt agtagatttc aaaaatacgg ttctgatcat gaccagcaat atcggttcac
    30361 agttactgct ggaaggcgtg actgctgatg gaacgattcc agaagcagtg gctgaacaag
    30421 tcaacacgct attacgtgga aacttcaaac cagaattctt gaaccgaatc gacgacacga
    30481 ttttatttac accattgagc ctagacaacg tgaaaggaat cgttgacaaa atggtggcgc
    30541 aattggcaca acgtctggaa catcaagaaa tcctgctgac aatcagtgat gaagccaaaa
    30601 cgtggattgc tgaaaatgct tatgaaccag catatggtgc aagaccattg aaacgattta
    30661 tcactaagga agtagaaaca ccactagcta aagaaatcgt tgcaggacat gttatgccta
    30721 aatcaaaagt aacgattact ttacttgatg gacaacttca tttcaaaaca gaagagctag
    30781 aagaaatcgt ctaatcttcc attgagacta taaaagaaaa aaggggtctg acacaactag
    30841 tgtcagacct ccttttttca aataaacagt gttccagaag tagtttcttc gaaaaataag
    30901 ccgaaatttc ttgaagctgc ccgcttctgt cacaacctct tttattaggt atttttcact
    30961 ttttctaatt tataactttc aaagtaacgc tcatcttttt tactttgaga ccatgcaaac
    31021 aatcccatta aggcagcacc tatcgtattt gtcagcaagt cgcccatcgt atccatcaat
    31081 gctgctcgtc cgacaaacag tgtgccatca gaagcagcaa aacgttgaag attcatgcct
    31141 aagaactggt cgcataagaa ttcccagaac tcccaaaaca cgccgcaaag tccagcaaac
    31201 gcaaagccga ataagaggaa caaccaagga ctggtttttg atatctcagc atctttcatc
    31261 aagtaaccaa tcaatccata gccaagagct gtcaggacca ttggactcac cgcatgtaag
    31321 attttgtccc aaaaagagat gatactgatc aaatgcatcc ctgtgcctaa aaagacagag
    31381 atgaacaaga aaaaccagta aaacaaaacg attgctgctg gtattttaag tttgaaaact
    31441 ttcaaaatga cttgtggcat gaaaatcaat gcaatcccta ataaagattc cacgataaat
    31501 gtgattcctt gagaagtcga atatttttgt gtgataaatt cataaaaatt atagcagaca
    31561 acgaatacac caaaagcaag taaagctatt tgcacctttt tgatttcttt aatcactgaa
    31621 agtccctcca tctaaaaatc tttttacttc tgaaagcaac tcaatttctg aagaaaccac
    31681 ttttccgttc aatttgatca aacctattgt atataagttc aggtagtgaa attgattttc
    31741 agctgtttct tgtagtgcag cgattttttt cgggtgggtt ccgccttgct gtcgagtatc
    31801 agtatataag ccgatcactg ggatgccttt cgcataagcc acaccgattt ccgatgctac
    31861 cccggcatcg atcgtcagcc catctagtaa agcaatcatt aaatcactag ccaacacttt
    31921 ttctgtgtca gcaagagcaa tcattcgact gtctgcataa gcagttttgt cattgattgc
    31981 ttcattttct tgaggaagat agatcgaaag ctcagaagag agctctctga tttctttaac
    32041 taactgttgg ttatataaca aatcactttt agcaaacatt ggtgcggcaa aataaatttt
    32101 catctaaaca tcatcctttt tatcaaaaat aatcgctaaa gtaagcttcc atgtttggaa
    32161 tgatacgctc tagtgacttc agtgtttctt catctgtttc taaccgttcg attcttgcca
    32221 aataagaagc acggcgataa ttcatcatca agcaagtcaa tgtttggata tccagtcgaa
    32281 cggattttcc tataggttca tttaagacac ggacttgtcc ctgttcatcc catgttaaag
    32341 cgaagacacc attattccaa ggagcgacag gatcttcaat gatgaaatga aaagcatcag
    32401 ctgttccaac aaacggaaac cgttgaagga attcttttac atcaacgatc cgagccataa
    32461 aatatggttc gatctgctct ttgatctgac tatcttcaat caaaaatgct aaaggctcat
    32521 ttttataaat atctccatgt acccagtaga tcatggaaaa atgtgcggaa ataaagttcc
    32581 ataagccatt tcgagcttct tgatcaagat agaacatttc ttttacgtga aatatttctt
    32641 cagctaccca gtagaacaga acacctcttg gttcatggtt gctatcgtag tagacagcgg
    32701 ctgtgcgctc atcttcattt tcgaaacgcc aatactcttc ccaatgaaaa tcactgcgga
    32761 ataaagcacc atggttttgt tgggcaaatt tgtcataaac ggaaaatacg tctggatgat
    32821 caactggtaa tcgctcaacc ataccactta ctgggatttg tttaggtagc tgtgtatctc
    32881 tgattttaaa ggaaagttta tcagacatga tttcccatcc tttacgccga tagtagggga
    32941 tactgtaagg atacaaataa gagatccatt gcttattctt gcgcatatta tccaatgcaa
    33001 cgataattag atcttgcatc aatccatggt tagcatattc tggataagtt ccaactccag
    33061 tcacaccacc cattttataa cgtgcaccgt gaatattgac ttcacaagga tagatagcga
    33121 tttgagagat cagtttattt tcgtggaacc atccgaacac tttcgatact tccaaaatcg
    33181 gttttttgga acggataaaa gctcgtttat tctcaaaccc gctttcctcg atgtctgctt
    33241 cagtcacttg gaacacatag gaaagcaatt cattgaattg atcgatatgt tcttgagtga
    33301 ccggttttaa ttcaagattt tctctaaaat ctttttgatc cattttttca tctccattta
    33361 ttagcttgat aacagtatag caaaattcta caaaaaaaac ttctatagct gactaattaa
    33421 aaactcttta aattactgct tttcgttggt ataatggaaa gtgctgatta tatgaaaaga
    33481 ggagacgcta tggatataaa gaaaatgaaa gaacgccaag agcagattcg taatttttcg
    33541 atcatcgctc atatcgacca tgggaaatct acattggccg atcgtatttt agaaatgacg
    33601 cacactgtga cgtctcgcga aatgcaagat cagctgttag actcgatgga tttggaacgt
    33661 gaacgtggaa tcacgattaa attaaatgct gtagaactca attacacagc gaaaaatgga
    33721 gaaacatata tctttcactt gattgacacc ccagggcacg tcgacttcac ctatgaagtc
    33781 tctcgaagcc ttgctgcctg tgagggtgcc gttttagtgg tcgatgcagc acaaggaatc
    33841 gaagcacaaa cattggccaa tgtttattta gcgttagaca atgatttgga gatcctgcca
    33901 gtcatcaata aaatcgacct acccgcagca gatcccgaac gtgtccgtca agaaatcgaa
    33961 gacgtaatcg gaatcgatgc tagtgaagct gtattagcca gtgccaaatc agggattggg
    34021 attgaagata ttctcgagca gatcgtggaa tatgttcctg caccaagtgg agacttagat
    34081 gcaccgctaa aagctttgat ctttgactct atttatgata gttaccgcgg tgttgttcta
    34141 aatgtccgga tcacggacgg tatggtcaaa cccggagaca aaatcaaact gatgagtaat
    34201 ggcaaaacat ttgatgttac agaggtcggg gtcttctcgc caaaagcagt tgctagagac
    34261 tttttgatgg tcggtgatgt cggttatatc acagcaagta tcaaaactgt ccaagataca
    34321 cgagtagggg atacagtaac gttagcagac aatcctgccg ctgaagcttt accaggttac
    34381 cgaaaaatga atccgatggt ttattgtggt ctttatccaa ttgatacttc tcgttacaac
    34441 gatttgcggg aagctttaga aaaactgcaa ttgaatgatg ctgctttaca attcgaacca
    34501 gaaacctctc aagcattagg ttttgggttc cgctgtggat tcctcggact tcttcatatg
    34561 gatgtcgtcc aagaacgctt ggaacgggaa ttcaatctag aattgatcac gactgcacca
    34621 tccgttattt accatgttaa taaaacggat ggtacgactg ctacagtaga taacccggca
    34681 gattttcctg aacctgtaac gattcaggat gtagaagaac cattcgtcaa agcacagatc
    34741 atggtcccaa atgattttgt cggtgcagta atggaacttt ctcaacgaaa acggggagaa
    34801 ttcattacga tggattattt agatgattac cgagtaaatg tcgtctataa tattccgctt
    34861 tctgaaattg tctttgactt tttcgacaaa ttgaaatcaa gcacgaaagg ctatgcctca
    34921 ttggattatg aaatgtctgg ttatcaaaaa agcaagttag tgaagatgga catcttgctg
    34981 aatggagaaa aagtcgatgc attaagcttt atcgttcacc gagactttgc ctatgaacgt
    35041 ggaaaagcaa tcgttgaaaa actgaaaaaa ctaatcccac gccaacaatt tgaggtgcca
    35101 atccaagcag ctattggaca aaaaatcgtt gcccgttcag acatcaaagc cttgcgtaaa
    35161 aacgtcttgg ctaaatgtta tggtggagat gtttctcgta aacgtaaact cttagagaaa
    35221 caaaaagaag ggaagaagcg gatgaaacaa atcggctccg tcgaagttcc gcaagaagcc
    35281 ttcatggccg ttctgaaaat ggacgaggac gaaccgaaga aatagcaagg gtttgtttga
    35341 aaacagtgta aaaaataaac catgtttaca tggatttgcc accggtttgc caccggtg
//
LOCUS       DARBFO010000017        34869 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000017 DARBFO010000000
VERSION     DARBFO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.018"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(59..187)
                     /locus_tag="SCK51_001007"
     CDS             complement(59..187)
                     /locus_tag="SCK51_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEG1251649.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="SCK51_001008"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="SCK51_001008"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEG1251650.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="SCK51_001009"
     CDS             complement(1674..2729)
                     /locus_tag="SCK51_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEG1251651.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="SCK51_001010"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="SCK51_001010"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEG1251652.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="SCK51_001011"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="SCK51_001011"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEG1251653.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="SCK51_001012"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="SCK51_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEG1251654.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="SCK51_001013"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="SCK51_001013"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEG1251655.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="SCK51_001014"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="SCK51_001014"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEG1251656.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="SCK51_001015"
     CDS             10491..12050
                     /locus_tag="SCK51_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEG1251657.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="SCK51_001016"
     CDS             complement(12161..14092)
                     /locus_tag="SCK51_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1251658.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="SCK51_001017"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="SCK51_001017"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEG1251659.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="SCK51_001018"
     CDS             complement(15021..15773)
                     /locus_tag="SCK51_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1251660.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="SCK51_001019"
     CDS             complement(15932..16141)
                     /locus_tag="SCK51_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251661.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="SCK51_001020"
     CDS             complement(16152..17480)
                     /locus_tag="SCK51_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251662.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="SCK51_001021"
     CDS             17616..17771
                     /locus_tag="SCK51_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251663.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /locus_tag="SCK51_001022"
     CDS             complement(17845..18432)
                     /locus_tag="SCK51_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEG1251664.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="SCK51_001023"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="SCK51_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEG1251665.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="SCK51_001024"
     CDS             complement(20012..21301)
                     /locus_tag="SCK51_001024"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEG1251666.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="SCK51_001025"
     CDS             complement(21455..22387)
                     /locus_tag="SCK51_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251667.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="SCK51_001026"
     CDS             complement(22544..23203)
                     /locus_tag="SCK51_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG1251668.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="SCK51_001027"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="SCK51_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEG1251669.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="SCK51_001028"
     CDS             complement(24244..24534)
                     /locus_tag="SCK51_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251670.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24943..26748
                     /gene="glmS"
                     /locus_tag="SCK51_001029"
     CDS             24943..26748
                     /gene="glmS"
                     /locus_tag="SCK51_001029"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEG1251671.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26820..28175)
                     /locus_tag="SCK51_001030"
     CDS             complement(26820..28175)
                     /locus_tag="SCK51_001030"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEG1251672.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28199..29368)
                     /locus_tag="SCK51_001031"
     CDS             complement(28199..29368)
                     /locus_tag="SCK51_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251673.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29365..30252)
                     /locus_tag="SCK51_001032"
     CDS             complement(29365..30252)
                     /locus_tag="SCK51_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEG1251674.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="SCK51_001033"
     CDS             complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="SCK51_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEG1251675.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            34332..34853
                     /locus_tag="SCK51_001034"
     CDS             34332..34853
                     /locus_tag="SCK51_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEG1251676.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         9400 a         7569 c         6284 g        11616 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcctgc ttttttgaag taagcgtcat aataacccgc gcttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tcagagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 aattggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcctcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgtttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttccgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgatagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcgattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg cgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ctccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggaa attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat gtcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt taccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccggtcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatacgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaatgtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtgggg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagaggc tgggaaaacc cagcctcttc ttttttattt ctatgcttct gctactgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgccg tgaggagcca
    12301 gtaatttgat tccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcaactc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcaaagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccagcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagtaat tttttcccat acggaatgat ttcttctaca gattggaagg ttgtgttgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaac ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagctggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cctaagccat tgatctcagt ttcagtgtct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg gatccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattcaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctgcgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctacta gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcag aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgtga tcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt ggatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttcaaatc tttttataat ttaattataa
    17521 ccaatgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc gccctgaggt ggcacacttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcaagttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttaaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttctttt aacacggaac
    21661 taagcagcgc atcttcattc ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgcc actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcata ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaacgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccca
    22621 gatgttgctt tttctgatta aagactgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttgg ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagagac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaggc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgtata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttggagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt cttttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgttaatt catcttctac
    24361 cgtaaactca cgagcttgag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaaggacat ttgacaaaga atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaaagga ggatagttat accttttagg atagcgccag gtctgtgtaa
    24781 acagatgacg aggagagggt ttatcgaaag attcggcgga tggccctagg gactgcactc
    24841 tacagaaaat ccacaaaaaa tgtctgcaaa gataaaacaa aaggattttc ctgcagctgg
    24901 aagcattcat acgaattcat tatagaaagt aggatgtttt ttatgtgcgg aattgttgga
    24961 atgattggat taaaaaatgt aacaccagga ttgattgacg gtctagaaaa attagaatac
    25021 cgtggttatg attcagctgg tatctttgtc tcagatggca caactgatta tctggtcaaa
    25081 gcacaaggca gaattcaaaa tttgaaaaac aaaataaata ttgatactac aggaaatata
    25141 ggtattggtc atacacgatg ggcaactcac gggcaacctt ctgaagaaaa cgcccatcca
    25201 catacttccc aaagtggacg cttcgtgctc gttcacaacg gcgtaatcga aaactttgaa
    25261 gaacttaaaa ttgcttattt agcaaatgac cactttattg gtgagaccga tactgagatt
    25321 atcgctcatc taattgaaac tttcgcgaag gatactacaa cgcaagaggc gtttttaaaa
    25381 gctttgcggg tcatcaaagg ttcttacgct ttcgctttga ttgatcggac agcacctgat
    25441 gttatttacg tcgctaaaaa taaaagtcca ctattaatcg gactcggcga tggattcaat
    25501 gtgattgcca gcgatgcaat ggctatgctt gctcatacga aggaattcgt tgagattgaa
    25561 gatgaagaga tggttaccgt cacatcagaa aaagttatta ttcaaaattt tgcaggtgac
    25621 atcgtagaac gttcttcatt cgaagcacaa gtagatgctt cagatatcga gaaaggtacc
    25681 tatccttttt atatgctgaa agaaatcgac gaacagccta ttatcatgcg tcgtatcgct
    25741 caaaaatacg taacagaaga caatcgtgtc tctcttgatc aaaagctggt cgatacattg
    25801 tctgatagcg atcgaatcta tatcgttgct tgcggaacta gttaccatgc ggggttagca
    25861 ggtaaacaga ctttggaaaa attgacacag attcctgttg aggttcattt agccagtgaa
    25921 tttggctata acacacctct tctttcaaaa aaaccattct ttattttctt gagtcaaagt
    25981 ggcgaaacag cagacagccg ccaagtgctg gtaaaaatca accgtctcgg ctacccttca
    26041 cttacgatta caaatgttgc tggttctact ctttcgcggg aagcatcctt tacactgcta
    26101 ttgcatgctg gacccgaaat cgcagtagca tcaacgaaag cttatactgc acaaatagct
    26161 gtactaactt tattagccaa agctatcggt gataaaaaag aatttgaaac ttctcttgta
    26221 ttcgatatca ctcatgaatt gagtttagtt gctaatgcga tggaatcggt catcgcccaa
    26281 aaagattatt tagaagagtt ggcagaagta tatctcagcg atacaagaaa tgccttttac
    26341 attggacgtg gtgctgacta caacgtttct cttgaagctg ctttgaaatt gaaagaaatc
    26401 agttatatcc aggcagaagg ttttgccgct ggcgaattga aacatggaac gattgctttg
    26461 atcgaagaag gaacaccagt gatcggaatc atcagtgaag aagtgactgg tgcgcatact
    26521 cgaggaaacc tgaaagaagt cgaaagccgt ggcgctaaaa cactagttat tgtctcagag
    26581 ggattagaaa aggaaagtga ccagctggtg ctaccaactg tccaccctta tctaactact
    26641 ctaaccacag tcgtaccgac tcaattactt gcatattacg caacgctatt acggggattc
    26701 gacgttgaca aaccacgtaa tttagcgaaa tcagtgacag tagaataata aaagaacaag
    26761 gaccagtgtc agacatctga cactggtcct tgttctttta ttttttatga aaatatctct
    26821 taatccagtc cgatttcttc tttgactact gccgcgattt tatcgacata gtaattaact
    26881 ttctcatcag taggcgcttc tgccatcact cgaagtaatg gttctgtacc ggaaggacga
    26941 actaggattc ttccttctga tcccatttca gcttctgcct cttcaatcac ttttttgatt
    27001 gccggaacat ccatcgcacc gtttttatca cttacgcgga tattcactaa tttttgcgga
    27061 tagattgtta cctctgctgc aagctctgat aatttcttac ctgtctgttt catgatattc
    27121 agtaactgaa ttcctgaaag catcccatct ccagttgtgt taaaatctaa gaaaatcatg
    27181 tgcccagact gctcgccgcc aaagttataa tcgtttttgc gcatttcttc tacaacgtaa
    27241 cgatcaccaa cttgtgtaac gacatctttc aaaccgattc cttccaccgc tttgtggaat
    27301 cctaagttac tcatgacagt cgtaacgatc gtatcttttt tcaatcgatt tttttctgcc
    27361 aagtatttag cacagataaa catgatttta tcaccgtcga caatgtttcc aagttcatcc
    27421 accgcaataa tacgatcgcc atcgccatcg aaagctagtc cagcatctgc tcctttttca
    27481 acgaccatct cagctaaacg ttctggatgg gtagagccta cgccgtcatt gatatttaac
    27541 ccgtctggat ttgtgcccat tgtataaaaa tctgtttcaa gatcggcaaa caaacgatta
    27601 acacttgttg aagttgctcc gtttgctgca tcgacacaaa tggtcaaacc ggaaaggtca
    27661 ccgttgatcg tttgtacaag gaattgagaa tatttcaata agccttctgg aaattcttct
    27721 acagttccca atccttcagc tgatggacga ggcaattcat ccacttcagc atctaataat
    27781 gcttcaattt ctagctcttg gtcatctact agtttaaaac catctgcacc gaaaaatttg
    27841 attccattat cttgtgctgg attatgagaa gcagaaatca tgacccctgc gcttgctttt
    27901 tgcaaacgtg tcagataagc aacacctggt gtagagatca cacctagctg gaacacttcg
    27961 atacccaccg ataaaagtcc tgaaatcagc gcttcttcta acatttgacc tgagattctt
    28021 gtatcccgcc ctactaacac gcgtgggcgt tttccttctt cttcatgctg gcttaggaca
    28081 taacctccgc accgtcccag cttaaatgca agttctggcg taagttcctt gttggcttca
    28141 ccgcgtacgc cgtctgttcc aaaatattta cccatagttg taaaattact cctttaactt
    28201 aattttctga ggaatttgtt ctgctcattt gtgttgattc gcttgtttct gatgtttctt
    28261 ctgtacttgt ggtcgagctg cttgtcccag attccgaact ggaagaaatg gttgttgtcg
    28321 atggttccgt tcctgaatca gcagatgaaa tacttctact cgtacttgta ttttcttgtg
    28381 tccgactgct cgtcgaagca gagctactgc ttgacgtttg caacacgggt gtaatctcca
    28441 cactaactga ttcgggcttg atacttaacc cctcttctac tggtatttca attttacgag
    28501 ttgttcgatg attgatacct gagacattga tcggtacagc aatactgtca ccgatcgatg
    28561 caagcaaatc gctagagccg cttacttctg cttccagttt tgaaaaactg aaatcatagc
    28621 tttctacccc ggcagctggt gttccttgct gtatcccata aagtcgtacc gacttgcttg
    28681 gcttgatcga atcagctctc actgttactt gcactggatc agagacgata cttaatactt
    28741 cccctgctgc attcagcgcc tgtattggga cctcactatt aatcccatca tctgtgacca
    28801 tcgaagcgtc cacatcagct actactcgtt tgatttctgc caatgttttg tcacctgtag
    28861 tgatagagac ttttttagga ctaacggaag tatcatcaag ttcatatcca gatggtgtga
    28921 acgttgaagg aatcaacgtt tccacatcga aatcttttat tacctttttc tcaaccgtta
    28981 ctgtaatagt cgatggttcg atctttgctg tgacagcact gctcaaattt tgaattttta
    29041 gcggaacttc atgtgttcct tcgcccagct tagtgatatc tgctgtgacg cggaacatcc
    29101 gtgtatcttc gtttgcctct gcattcaact gaactcggtt tgcactggaa agtttgacag
    29161 tcactgcatt ttcatatcca tggatatagt agcgctttga atcataagaa atcttgatcg
    29221 gcacccccgt aactgtttga ttatacgctt catttccaga aagcgtattt ggaacggact
    29281 ggccatttgc attgaagtac agcgcaatac tgaacagcaa tgcaataaaa ctgtaaagaa
    29341 tgcttgtccg tttctctttt gagatcattt gcgatcccct cccttagtca taccgtctag
    29401 gaaagattga atcacgtttt tcttgttttt tgttttctct tcagcaatta gttctctacg
    29461 caagattgcc aaatattctt gctgggataa gccagccatc agttcattat ctaacgtgat
    29521 gctgacgtcc ccggtttctt cagagacaac gatggtaatc gcgtcactga cttcgcttat
    29581 accgacagct gcccggtgtc gagtcccgaa ttctttaggg atcaacatac tttctgatag
    29641 aggaagatag gcgcttgcta ctgcgatctt tccttctttt acaataactg caccgtcatg
    29701 cagcggtgta ttaggaataa agatattgat cagcagctcc ccagtgatat ctgcatctaa
    29761 agcaatccct gtctcaatat actcatctaa tccagtatga cgttcgatag tgatcaacgc
    29821 accgattttt cgcttggaca tatattggat ggctttgtca aaagacaaga tcatcttctc
    29881 atcttcctgc tgttcgcttt ttgattgttt gaaaagcgaa ctgcgtccaa gatgttccag
    29941 tcctctccgg atttccggct gaaaaatcac gatagcagct atcaccccgt acgtaatgac
    30001 ttggttcatc agccaagaca aggtgtgcag accaatgatt tccgctacga ttcggataac
    30061 aataaaaata gctacacctt tcagaagctg aatcgctttt gtgcctcgca gcagttggat
    30121 cagtttgtat acaagatacc agacaaccag aatatcaagt atgttcacca aaaaattcag
    30181 agagaacaag tcaccggaaa acatttgctg ccaatagtcc aaattaaaaa gttccccgat
    30241 ttgaaatgac ataaaaagcc tctcttctct tttgagattt cctctctagt atatcatatg
    30301 ttttaatttt agaggctgat atgctcgtat ttttcttgag aaaaacgcat aattttcaag
    30361 aaaattgcaa gtatgcgttt tatttttctc tgcaataaat atttttaaac ttcagagaat
    30421 tattcattat attttttctg ttatttttcc tttttttctt ttgtagcttg ttccattttt
    30481 ttacgtcgtt ctgctctggc agctcttcta gcagctcttc tttcttctgc ttcaaggtcc
    30541 gtcgtttttt cttttctagt cttaacgggt acatcgatag attcctctgg ttcaagtcgt
    30601 ttcttgattt tttctaattc tcccgtcagt gcagcatagt tgtaaaaacg tgttttagcg
    30661 tcgtggacag tcttttcaaa taaagctttt gcaagatttg gatttgcgtt atctagcgag
    30721 gaaaagcgaa cttgtttcct catgaaatca ggcatagcag agaaatcagg ttttttgtag
    30781 tccaaagtca ttgggttctt gtttttttcg accattgtag ggttatatcg atataatgac
    30841 caataaccag aagaaacagc ttcttttgct tctttaagcg attctttcat tccgccaagc
    30901 aagccatgtg tgatacaagg cgtataggca ataatcagag aaggaccagg atacttttct
    30961 gcttcttcaa atgccttgat cgtttgcatt tgattggcac ctgaagcaat ttgcgccaca
    31021 taaacatttc catatgtcat cgccatcatt cctagatctt tctttgaagc atactttcca
    31081 ctagccgaaa atttagcgat tgccgaagct ggtgttgctt ttgaggtttg accgccagta
    31141 ttggaataga cttcgttatc caaaacaagg atattcacgt ctgctccgct ggcaagtaca
    31201 tgatcgatcc cgccaaaacc aatgtcgtaa gcccatccgt ctccaccaat catccattgg
    31261 ctgtttttta cgaatagatc ctttctcttg tatatatcct ccagttcagg gaaaattgat
    31321 cgttcgcttt cgatcgcagc ttgcagtttt gctgctcttt gctgcgttcc ttcactctca
    31381 aacatgtgta ctaaccagtc ttccatcaat tctcgtagtt cttctgatac ttttggcaat
    31441 acttctttga ttgaacttgc caaagcctcg cgacgagtct gtgccgctag aagcatacca
    31501 taaccaaatt cagcattatc ttccaataaa gaatttgacc atgcaggccc ttgaccacat
    31561 tcattggtcg tataaggaga agcaggagaa gcaccgcccc aaatagaaga acagcctgtc
    31621 gcattcgcga tcatcattcg atcaccgaac atttgtgtca ataattttac ataaggtgtt
    31681 tcgccacagc ctgagcaagc gcccgagaat tcaagaagcg gttgattgaa ttgtgtagac
    31741 aagacagtat tctttccttt gactggattt gctttttgtc ttaatgtcat tgcaaaagcc
    31801 caattcacag cttgttcttt ctgttcttca taaggcttca tcactaaagc tttttctttt
    31861 gcgggacaag cctctacaca cagaccacaa cctgtacagt cttccactga aacttggata
    31921 cggtatttta cgccgtctgc gcctcgcatt tctctcacga taaatccttc tggcgcttct
    31981 tccatttcct cttcatctgc taaaaatgga cggatggcag catgaggaca aacaaaggca
    32041 cattcattac acatcgtaca gcgatcacta atccactcag gtacttctaa agcgacgccc
    32101 cgcttttcca tagaagccgt tcctaaaggc atttcgccag ttgtcattcc atttgctaca
    32161 agtgtaccaa cagagagttg atttccttcc tgtgcattca ctggttcaag tatttcttgt
    32221 atataaccag tgttcctttt attgattttt tcaggcatct ccaacgttct ccaatggtct
    32281 gggatcgcga cttgatgaag cagttcggtc gtccgatcga ttgcttgtat gtttttttct
    32341 acgatagcca ttgatttatg tccatagctt ttcagggctt cttcttttag cagcggaagc
    32401 acctcatcaa aaggaatgat gtcagctaat ttaaaaaaag ccgtttccat tgccgtattg
    32461 atccggcgtc ccaaaccgac ttcttgggcc aatctcatgg cattgatcgt atagaatcta
    32521 atgttatttt cagccaagta acgttttaat tttaagggca gatgcgtttc taattgttcg
    32581 tcgctccaaa gagtattcaa taaaaaaatg ccacctggct tcaatccttt gaccaagtca
    32641 tagctatgca ggtaagctgg tgtgtgacag gcaacaaggt ccgcatgctc gacaagatac
    32701 gctgaacgta tcggcgtatc tccaaaacga agatgagaga cagtcagacc gccagatttt
    32761 ttcgaatcat aataaaaata gccttgcgca tatttatcgg tgtgatctcc aataatcttg
    32821 atagctgatt tatttgctcc aactgttccg tcagatccaa atccccaaaa cttggcctgg
    32881 aatgtttgcg gttctgtcaa atccagactt tcctttggtt ccaaagattg ataagtcaca
    32941 tcatccacga tccctatggt aaaccgtttt ttgcgtatgc ttgggtcttt tttcaattca
    33001 tcaaaaactg ctgagatttg gtcgggagtc acatctttag accccaatcc gtagcgacca
    33061 ccaataacag ccgggcgaag ttcagaatca tataacgcac tttgaacgtc taataagaga
    33121 ggttctccac ctgcccctgg ttctttactt cgatcaagaa cagcaattgt ttttactgta
    33181 tgaggcaact tttgcacgaa tgtttccact ggaaaaggac gataaagatg gatattcaaa
    33241 aaacctgttt tccgtccgtt tgcattcaag taatcaatcg tttgttcaat cgtctgtgca
    33301 actgatccca tagaaacaat gacttcttcc gcatcgtctg ctccgtaata agtcaccaag
    33361 tcataagaag ttcctcgtaa tcggttgatc tctttcatat atttttgaac gatttcaggt
    33421 aatttatcat aataggggtt gatcgtttcc cgttgttgaa aatggatgtc tggattctgg
    33481 ttcgtaccac taacagtcgg atgattggga ttcatccctc gtttacgaaa tgaagctaat
    33541 ttctcctgat tgatcagagg agctaattct tcatattcca aaacttcgat tttttgaatc
    33601 tcgtgactcg tacggaatcc atcaaagaaa ttcaaaaaag ggacttctcc ttcaatagaa
    33661 gcaagatggg caacaggtgc taaatccatc acttcttgta cactgctttc agctaacata
    33721 gcaaatcctg tttgtcttgc agccatcacg tctccgtgat ctccgaaaat attcaacgca
    33781 tttgtcgtca ctgcccgact agcaacgtgg aaaacacttg gcagtagttc cccagcaatc
    33841 ttgtacatat tcggtatcat caaaagcagc ccttgtgaag cggtatatgt agttgtcagt
    33901 gcaccagttt tcaatgatcc atggaccaca ccagctgctc ctgcttcgga ttgcatttct
    33961 actactttta ctggttgacc gaatacgttt tcttttcctg ctgctgacca ttgatctact
    34021 aattcagcca tggtcgaact gggtgttatt ggatatattg cagcaatctc ggtaaatgca
    34081 taagaaatat atgcggctgc cgtattgcca tccattgttt tcatttttcg catgattcat
    34141 catccttgtt catattctct cttattatct gttccattat aacgtaaaac atgccatttt
    34201 tttcttttat ttataaaaat attttacatg tatctatttg aattggtcat ctaattttta
    34261 gacaaacagt ctaaatcgtt gccttttttt gtcagacatg tataaactat aagcgaaaga
    34321 ggtgttttaa catggatata tcagtgattg atgcaacgaa aacaaataca caaaaaggaa
    34381 tcctttatgg ttcatcgaat gctcctaaaa aaatggtcga attcatcaac ttagcttgtc
    34441 cgtattgccg acaatggttt gaagaatctt atgagctatt agaagaagca gtccaaagcg
    34501 ggcaattgca gcgagtgatc aaattgttcg ataaagaaaa agaaagctta cagcgaggga
    34561 acgtgatgca ccggtatctc acaatcagcg atggccaaaa agcgatcaaa gagatcaaac
    34621 aaatattcga tactcaagac gagtggaaac atctttctct ccaagaagta gcggattttg
    34681 cagtagataa actaaagctg acagaacaaa aagacgaaca gctgagccaa gctgtcatca
    34741 acgaagctga gcaagcacat attcgatttg ttccaactgt catcttgggt aaagaaatct
    34801 ttgatgaatc tatttcaata gaggaattaa aagaattgat ccaaacaaaa taaaaaaata
    34861 aggagcgtg
//
LOCUS       DARBFO010000018        34663 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000018 DARBFO010000000
VERSION     DARBFO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.019"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            618..1307
                     /locus_tag="SCK51_001035"
     CDS             618..1307
                     /locus_tag="SCK51_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1251677.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1294..2502
                     /locus_tag="SCK51_001036"
     CDS             1294..2502
                     /locus_tag="SCK51_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1251678.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2796..4067
                     /gene="serS"
                     /locus_tag="SCK51_001037"
     CDS             2796..4067
                     /gene="serS"
                     /locus_tag="SCK51_001037"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEG1251679.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4616..6100)
                     /gene="guaB"
                     /locus_tag="SCK51_001038"
     CDS             complement(4616..6100)
                     /gene="guaB"
                     /locus_tag="SCK51_001038"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEG1251680.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6605..7300)
                     /locus_tag="SCK51_001039"
     CDS             complement(6605..7300)
                     /locus_tag="SCK51_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEG1251681.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7390..8490)
                     /gene="ychF"
                     /locus_tag="SCK51_001040"
     CDS             complement(7390..8490)
                     /gene="ychF"
                     /locus_tag="SCK51_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEG1251682.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8499..8690)
                     /locus_tag="SCK51_001041"
     CDS             complement(8499..8690)
                     /locus_tag="SCK51_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEG1251683.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8711..9601)
                     /locus_tag="SCK51_001042"
     CDS             complement(8711..9601)
                     /locus_tag="SCK51_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEG1251684.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9588..10355)
                     /locus_tag="SCK51_001043"
     CDS             complement(9588..10355)
                     /locus_tag="SCK51_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG1251685.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10367..11080)
                     /gene="rsmG"
                     /locus_tag="SCK51_001044"
     CDS             complement(10367..11080)
                     /gene="rsmG"
                     /locus_tag="SCK51_001044"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEG1251686.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11312..13213)
                     /gene="mnmG"
                     /locus_tag="SCK51_001045"
     CDS             complement(11312..13213)
                     /gene="mnmG"
                     /locus_tag="SCK51_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEG1251687.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13230..14627)
                     /gene="mnmE"
                     /locus_tag="SCK51_001046"
     CDS             complement(13230..14627)
                     /gene="mnmE"
                     /locus_tag="SCK51_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEG1251688.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14926..15309
                     /locus_tag="SCK51_001047"
     CDS             14926..15309
                     /locus_tag="SCK51_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251689.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15477..16235)
                     /locus_tag="SCK51_001048"
     CDS             complement(15477..16235)
                     /locus_tag="SCK51_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEG1251690.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16253..17071)
                     /locus_tag="SCK51_001049"
     CDS             complement(16253..17071)
                     /locus_tag="SCK51_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEG1251691.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17068..17424)
                     /gene="rnpA"
                     /locus_tag="SCK51_001050"
     CDS             complement(17068..17424)
                     /gene="rnpA"
                     /locus_tag="SCK51_001050"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEG1251692.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17542..17676)
                     /gene="rpmH"
                     /locus_tag="SCK51_001051"
     CDS             complement(17542..17676)
                     /gene="rpmH"
                     /locus_tag="SCK51_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEG1251693.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18199..19533
                     /gene="dnaA"
                     /locus_tag="SCK51_001052"
     CDS             18199..19533
                     /gene="dnaA"
                     /locus_tag="SCK51_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEG1251694.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19731..20861
                     /gene="dnaN"
                     /locus_tag="SCK51_001053"
     CDS             19731..20861
                     /gene="dnaN"
                     /locus_tag="SCK51_001053"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEG1251695.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21090..21332
                     /gene="yaaA"
                     /locus_tag="SCK51_001054"
     CDS             21090..21332
                     /gene="yaaA"
                     /locus_tag="SCK51_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEG1251696.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21319..22443
                     /gene="recF"
                     /locus_tag="SCK51_001055"
     CDS             21319..22443
                     /gene="recF"
                     /locus_tag="SCK51_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEG1251697.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22440..24386
                     /gene="gyrB"
                     /locus_tag="SCK51_001056"
     CDS             22440..24386
                     /gene="gyrB"
                     /locus_tag="SCK51_001056"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEG1251698.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24406..26877
                     /gene="gyrA"
                     /locus_tag="SCK51_001057"
     CDS             24406..26877
                     /gene="gyrA"
                     /locus_tag="SCK51_001057"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEG1251699.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27113..27412
                     /locus_tag="SCK51_001058"
     CDS             27113..27412
                     /locus_tag="SCK51_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEG1251700.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27462..27974
                     /gene="ssb"
                     /locus_tag="SCK51_001059"
     CDS             27462..27974
                     /gene="ssb"
                     /locus_tag="SCK51_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG1251701.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            28000..28236
                     /locus_tag="SCK51_001060"
     CDS             28000..28236
                     /locus_tag="SCK51_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEG1251702.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28379..30352
                     /locus_tag="SCK51_001061"
     CDS             28379..30352
                     /locus_tag="SCK51_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEG1251703.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30358..30810
                     /gene="rplI"
                     /locus_tag="SCK51_001062"
     CDS             30358..30810
                     /gene="rplI"
                     /locus_tag="SCK51_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEG1251704.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31084..32451
                     /gene="dnaB"
                     /locus_tag="SCK51_001063"
     CDS             31084..32451
                     /gene="dnaB"
                     /locus_tag="SCK51_001063"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEG1251705.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33231..34523
                     /locus_tag="SCK51_001064"
     CDS             33231..34523
                     /locus_tag="SCK51_001064"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEG1251706.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10561 a         6739 c         6847 g        10516 t
ORIGIN      
        1 ttcagttttc aaaggtctac ttcatcagga gtgtttcccg attcgttttt ggctgccgta
       61 gcaactttta tatcatatca gagagacaag taattgtcaa ctctttttta aaagtttttt
      121 cgatttcgtt tttcgaaatc ttccagcaac tcatttatca taacttatcg tttgttattt
      181 gtcaagaact tttttgaagt ttttttcgat agaatcaagc ttcgcaagtg gttctttttt
      241 aacgactcag ttatcttatc atgtcatttc caacatgtca acaacttatt ttttattatt
      301 taataagtta ttggtgttct tttgaacaaa taataatata tcaacatcac atgtgaaagt
      361 caataagaaa atgcttattt atcaacattt ctagcttaca tgatcattgg ttagttctgg
      421 tcctttttcg ttcgtcttta taactccgag agacgtaaaa tccttttgtt ttcagtttaa
      481 tgttcatgtt cttgtcttat tcacaccaac tagatgtctc accttctaaa attttcctta
      541 acaaagtgct gcttttatct atttctgctt ttttttgcta gaataatagt aacaatatga
      601 ctctggagga tttatttatg aaaattttga ttgctgacga tgataaagaa atcgttgaat
      661 tattaagtat ctatgttcac aatgaggggt atgaagcagt aaaggcttat gatgggaaag
      721 aagcattatc taaaatcaga acaatccctg atattgaatt acttatttta gatatcatga
      781 tgcctgagat ggatgggatg caagtagtga aagagctacg aaaagaatcc caaataccaa
      841 tcattatgct gacagcaaaa actactgata tggataagat caaaggactg gtcgctggtg
      901 cagatgatta tgtgaccaag ccatttaatc cgttagaagt aatggctaga gtaaagtctt
      961 tattacggcg tacaaaaatg cagattgccc cagatcagcc tgatgtatta gaagtaaatg
     1021 cattaatcat caataaagac tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt
     1081 taacagcttt ggaattcggt atcctctact tgcttgctag tcatcctaat cgagtattta
     1141 gcgcggatga aatttttgag cgtgtttgga aacaagaaag tattgtctct gctaaaactg
     1201 tcatggttca cgttagtcat ttaagagata agattgaaga agcaactggt ggtgaaaaag
     1261 tcattcaaac cgtttgggga gtgggctata aaattgacgc aagatagtaa acgagcaact
     1321 gaaaaacgcc gtcggatcaa tttgacttca aaagaaatca gcgaattgtt agcggaagga
     1381 atcatcacga ttatcctttt gcttctatta aatgtttcta ttctggttgt tatcagttcg
     1441 gtgatcaaca gttcaccttc tttgacgaat gcgatttggg attcaaagaa tatttttgca
     1501 gaacgattga acactgattt gttctggaac ggccggaatt ttattattcc ctttttcttt
     1561 ctattagata ttggtgtttt gtattggcgc ttgattcgaa gatatcgcca aatgcaattg
     1621 cggcatatca tcagcgaact tcactacatc gcaaacggaa attatgatca tcgtatccct
     1681 tttgaactga gtggtgactt aagccgagtg gtaacaagta tcaatggatt ggtggatagt
     1741 accgtagcag cgattgaaga cgaacgtaaa atcgaaaaat caaaagatga attgatcacg
     1801 aatgtcagtc atgatatccg taccccactc acgtccatca ttggatacct aggactgata
     1861 gaagatggcc agtatcactc agaagaagat cttttaaagt atacgcatac tgcttatatc
     1921 aaagcaaagc agatgaaatc tttagtggac gacttatttg aatacactaa ggtacgccag
     1981 cctgctgtcc cggtgaattt ttctgccttt gacatgatcc agttgatcga acagttagca
     2041 gctgattttg agttggaagc ttctaaaaaa aatatccaaa tacttgttca atcaaaagta
     2101 gactccctta tcatggatgg cgataccgaa aaacttgtac gtgtcttcaa taatcttttg
     2161 acgaatgcgt tgaaatacgg aaaaggagca acgaagatcg taatcgaagt agaacgtatc
     2221 ggttcagaag ttgttgcaac agtcaaaaat aacggagcaa tgatcccaca acaggcaatc
     2281 gataatctat tcgaccgttt ttatcgtgta gaagaatctc gttcacaagc aactggagga
     2341 actggacttg gtttagcaat cgctcaaagc attgtggctt tgcacggcgg atacatttat
     2401 gcaaaatcag ataagcaatg gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa
     2461 aaattaccta ttgatacaca ggaagttatt gacgaaagct gaaatattcg ctattcttat
     2521 tacaattatt agaataaatg aaaaacacag aacccagtac actttattct ttttcagaga
     2581 gctgatggca ggtgtaaatc agcaaaggat aatttggaag ttccagttcg tttacgtgcc
     2641 ctttcgaaat aagaaaggtc ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg
     2701 ctgaacttgg gtggtaccgc gaatttcaga ctgattcgtc ccaagaataa tgggatggat
     2761 cagtcttttt ttattttcaa aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac
     2821 aaaatattga agaagtacaa gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag
     2881 ctgaattcat ggaactagat gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa
     2941 aaaaatatcg aaatgatgtt tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg
     3001 cttctgctaa aatcgctgaa atgaaagaag tcggtggcaa catcaaagca ttggatactg
     3061 aaattgctga agtggatgaa aaattgaaac aaattgcaac tactttgcca aaccttcccc
     3121 atgattctgt gccagttgga acagatgaag aggacaatgt agaaatccgt cgttggagcg
     3181 aaccacgtac atttgctttt gaaccaaaac cacactggga aattgcagaa aatcttgata
     3241 ttttagattt tgaacgtggc gcaaaagtag ccggtagccg ttttgtctat tacaaaggat
     3301 taggtgctcg attagaacgt gctgtctaca acttcatgct tgatcagcat atttacgaac
     3361 acggctatac tgaaatgatc actccttata tcgtcaacag taaagcgatg tttggtacgg
     3421 gacaatttcc aaaattcaaa gaagatgttt tccaattgga gaatactgat ctaacattaa
     3481 tcccaacagc tgaagtacca ttaactaact attataatga tgaaatttta gatggtgcag
     3541 atttacctat ttatttcact gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg
     3601 gtcgggatac acgaggactg attcgtcttc accaattcaa taaagtagaa atggtcaaat
     3661 tttcggatgt agagcattct tacgaagaat tggaaaaaat gacagctgat gcagaagata
     3721 tcttgaaaaa attgcatttg ccttatcgtg tgatggcctt atcaactgga gacatgggct
     3781 tttctgcagc taaaacttat gacttagaag tatggatacc tgcacaagat acttatcgtg
     3841 aaatcagttc ttgttctaac tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc
     3901 gcgataatga tggaaaaatc cactatactc atactctgaa tggttcagga cttgcagttg
     3961 gccgtacagt agctgccatt ttggaaaact accaaaatga agatggttct gtcacagttc
     4021 cagaagcgct cgttccttat atggggaatc tgaaagttat taaataaaga ggctgtgaaa
     4081 aagccgctta gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc
     4141 ataaattgga cttaataccg agaaactggc ttttgaacac catttatcga gaggttgtga
     4201 aatcagcgaa ttaacctgag tattagagaa aaaaatggaa aaatagtccc tcatatttta
     4261 ccgtttgttg aactaatacc gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt
     4321 aagcctaccc cccagctcct agttacaagg acaaattcaa aaaacagctc ttcatatttt
     4381 atgaatgttg tacttgtgat tcggaggtgg caagggaaca ctgtttattc aaaaagaggc
     4441 tgggacagaa gcgtttaact ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa
     4501 tttttgtgaa tttcttctta tttccgaagg agttgcttct acttccgccg tttatacgtg
     4561 tttagagcgt gagacaaaag tgttctttac ttttgtccca cgctctttaa tgtttttaag
     4621 attcaactga atagtttggt gcttcttttg tgatttgtac atcatgaggg tgtgattctt
     4681 ttaatccact gccgctcatt tggataaatt gcgcttcatc acgtaattgc tgtagattgg
     4741 ctgccccaac ataacccatt cctgaacgca aaccgccgat catttggaaa ataatatctg
     4801 caacacttcc tttataagca acccgacctt cgattccttc tggtactaat ttatttgctt
     4861 cattgacact accttggaag taacggtctt ttgaaccttt ttccatcgcg cctaatgagc
     4921 ccatacctcg gtatgttttg aatcgacgtc cttggaagat ttcgaattca cctggtgatt
     4981 catctgtccc tgccaacata cttcctagca tcactgcatg tccgccggca gctaatgctt
     5041 tgacgatgtc accagaatat ttgatccctc cgtctgcaat aatcgctttg ccatattgac
     5101 gagcaactga tgctgcatca taaatagctg tgagctgagg tacacctaca ccggctacca
     5161 cacgtgtcgt acaaatcgat cctggtccga taccaacttt tacgacatct acacctgcat
     5221 catataaagc ttttgtcgct tcagccgtcg caacgtttcc tgcaatcaat gttgcatctg
     5281 caaaagttga acggatttct tgaatttttc ggatcacacc tgcactatgt ccatgagctg
     5341 tatcgatgat gattgcatct gcccctgctt ctaataaagc attcgcacgc tcaaatgtat
     5401 cgcttgttac accaacggct gctgctacaa gcaatcgacc atgttcatct tttgctgcat
     5461 ttggaaattc gatcactttc tcgatgtctt tgatcgtgat caatccgctt aaacgacctt
     5521 cgttatctac gattggtaat ttttcgattt tatgtttttg caaaatcttt tctgcatctt
     5581 ttagagaggt acctactggg gcagtcacta agttgtcttt tgtcatcaca tcagcaatcg
     5641 gcatgtgata atctgttaca aaacgcatgt cgcggtttgt aattatccct accaattttc
     5701 tgttttcaag tgtttcaacg attggcacac cactgatacg atatttgctc atcaattctt
     5761 ctgcgtctgc tacaagatgc tgtggcgtta agaagaacgg atcaatgatt acgccacttt
     5821 cagaacgttt gacttttctt acttcatccg cttgctggct gatcgtcatg tttttatgga
     5881 tcacacctag tccgccttgt cttgccatag caatggccat cttgctgtcg gtaacagtat
     5941 ccatacttgc actgatgatt ggaatattca acttaatatt tttagctaat tgtacgctca
     6001 tatccacttc gtttggtaac acgtgacttt ctgctggtat caataagaca tcatcaaaag
     6061 taaatccttt tttcgtgaac tttgtttccc agttggacat ttttagtacc actcctctaa
     6121 tttagtttat ctattaaaat aattgatttt ttcatttaag tcaatgatct acttggaatc
     6181 aagttagctt tctcatagaa taccaatata aagcgtttac atctgtgtat attaaaagag
     6241 attgtaaaaa agctgtcctg catcaagtaa taagaacagc caatcaaaaa cagcttctca
     6301 tgtttttgaa ttggttgggt ttattaccgc agatgcaaac ttttgaacac cgtttagtgg
     6361 agaggctgtg aaaaagctgc ttagtttcga gtagtaagaa aaattttgtg aaaatggctt
     6421 tccacatttt tgcataaatt ggacttaata ccgagaaact ggcttttgaa caccgtttat
     6481 cgtaaaaagg gactgtgaca agacttttgt cacagtccct tactattatt taactatgga
     6541 taaacaaaat gaggttcttt actaccccta gatatctagc aaaatagctg atctttttgc
     6601 tagtttatct tcttgggacg gataaacttc caataatccc gtattttttc ttcaagtagt
     6661 aacggattgc taaagcagtt gccccgataa tgataactac tactggatct aatactggat
     6721 tgataactgg tggcaatagt gttgtcgcag aaaacagtac gacccaaaga agtgtcgttc
     6781 cacctaggat aagcaatgtc ttacccattc ctggtttctt gcttcgatct gctccgggat
     6841 attcatattg atagatgtat ttgtacatca tataaaagac ccagccaccg attgcagaac
     6901 ctagaacgag tgtagaaaag ccataagctg gcgttgttcc acgtgaaaac aatccggtaa
     6961 tcgcaatcat gatacttaat agaccaagca acagcaacgt attatctaac cacatcaata
     7021 aaggggtgga tgaatttttt acttctgctg gcttattgat gattgcttcc gttctttcag
     7081 agactgtccc aaacaattga cgggctgttc ttccgccttt ttgttctgca actaaagtag
     7141 gcagaatgtc atgaagggct tttacttttt cttcttcaga caaattcgct gcttctaaag
     7201 attttttcaa atcaaaaatg tactgttgat ttcttttggt taattttgtt tctaaactat
     7261 tgttttcttt tacgattgct cgtaattgtt ctggttccat tatctaatag tcctccttag
     7321 ccgttccttt tctcaacggt cgtcacaaaa gacattctac catattctac agctagtttg
     7381 atagcccttt tacacattaa agcggaataa cattacgtcg ccgtcttgta cgacataatc
     7441 ttttccttct aatcgaacac gtccagcttc ttttgctgca tgcatgtttc cgtaatgatc
     7501 caagtcttca aaagagacag tttctgcacg gataaatccg cgttcgaaat ctgaatggat
     7561 gattcctgct gcttgtggtg ctttgattcc ttttttgaat gtccaagcac gtacttcttg
     7621 ttccccagca gtaaagtaag tagctaagcc taacaaatca taagcagcac ggatcaactg
     7681 atccaaacct gattcttcga ttcctaaagc ttctaaaaat tccgctttgt cttcttcatc
     7741 taattctgcg atttcttctt ctgcacgagc actgaccacg atcacttctg cattttcttc
     7801 tgcagcaaag ttgcgcactt cttgtacata atgattcgcg tctgcatctg ctacgtcatc
     7861 ttctgagaca ttggctacat aaagaattgg ttttgtcgtc aataaaaata gtgatttaac
     7921 gattttttgt tcttcttcag taaattcgat cgttcgggca gattttcctt cttccagtac
     7981 aggtttgatt ttatccagta cagctagttc tgccactgca tctttatctt tggttttagc
     8041 aacttttgct acacgagtat agcgtttgtt gatagattct aagtctgcta acactagttc
     8101 caagttgatc gtatcaatat cttctagtgg atcaacacgt ccttctacgt gagtaatatt
     8161 atcatcatca aaacagcgaa caacgtgaca aatagcgtct acttggcgga tatggcttaa
     8221 gaattgattt cccaaacctt ctcctttact tgcgcctttt acgattccag caatatctgt
     8281 aaattcaaaa gtcgtcggaa cagttttttt cggatgtaca agttctgtta agcgttgcaa
     8341 acgccagtca ggtacttcta ccatccctac gttggggtcg atcgtcgcaa agggatagtt
     8401 tgctgcttct gctcctgctt ttgtgatcgc attaaacaaa gttgattttc ctacgttggg
     8461 caacccaaca attccagcag tcaatgccat aaatttattc actctttctc tgcatttttt
     8521 tcaattactt tttttaattt cttttcaaaa tctcgacggg tcatcatgac gatatgtccg
     8581 caattcatac atctgatttt gatatctgcg cccattcgaa tgacttccca gcgattcgtc
     8641 tggcaagcgt gaggtttttt catttcaacg atatcgtgca agtcgtacat aaaaatagcc
     8701 tcctttttgt ttattcttca tcaaattgaa tattcaagat atcaagaatt cgagtcaaat
     8761 ctttttgaga aagatattca atctcgatct tacctttccc atttttttct tggatagcta
     8821 cacttgtacc aaatttatcc atcaaacgat cttcgctttc tcggatataa tatggttttt
     8881 cctttgctag ccttgggatt tttttctttt cttttccatc attttgattt aactcagaaa
     8941 caagctgttc cagttggcgg acagttaaat tttcagttac tgcacgattg gctaatttca
     9001 agatcaatgt tttatttttt aaacctaaca gcgtacgggc ttgtcccata gataaacgct
     9061 gatcttgtac catttccttg acaagatccg gtaaagaaag caagcgcaaa taattggcga
     9121 tataaggtct gctctttcct aaacgactag ctacttctgc ttgtgttagt ttcaggtttt
     9181 tcatcagcat ctcatatgct tctgcttctt ctaacggatt cagatcttct cgctgcaagt
     9241 tttccagcac tgctacttgc atcatcgctt cttcgtcaaa gtctctaacg atagcaggga
     9301 tcgtttcttt ttcagctagt ttggacgctc gaaaacgtcg ctcccctgca ataatctcat
     9361 atccttttac tgcggaacgg cgtacgatga tgggttgaaa gacacctgat tgttcaatag
     9421 aattagctag ttcctgcaaa gatacttctt caaatgtttt acggggctga tacggatttg
     9481 gtcgcagctc attcaatggt agctggacaa cggtatcatc ttttacatcc accgtctcta
     9541 agttttctaa atcttgaaac agtgcatcga ttcctcggcc taatccttta cttttcttca
     9601 cgacttacca cttcctttgc caatgcttgg tagacttcag ctcctcgaga acgtggatcg
     9661 tagtcgatga tcggttttcc atgactaggt gcttctgata aacgaatatt tcttggaatg
     9721 atcgtttcgt aaactttttc gcggaagtat ttacgtactt cttctacgac ttcatttccc
     9781 agatttgttc gtgcatcata cattgtcagt aagactccct cgatctctaa ttctggatta
     9841 aagtgttttt ggactaaacg aaccgtattc agtaattggc ttaatccttc caatgcgtaa
     9901 tactcacact gaacaggtat caaaatcgaa tcacttgctg taaatgaatt gattgtcaga
     9961 tggccaagag aaggcgggca atcgatcaaa atataatcat actgactgct tacttctgct
    10021 aaagatcctt ttaatcttga ttctcttgcc atcattgacg tcaattcgat ctctgctcct
    10081 gctaattgga gagtcgcagg cacaatactc aaattttcgt gttcagtgat caaagttgcc
    10141 tcatcaattg gcaattcatt gactagaaca tcatagatgt ctcttgtcac atcaggtttt
    10201 ctgatcccga caccactggt agcatttcct tgtgcatcca tatccactag caatactttt
    10261 ttgccaaggc tagcaagaca agcacctaga ttcaccgtcg tcgttgtttt gccgacgcca
    10321 cctttttggt tcgcaacaga tattatttgt gccatctctt ctcctcctat ttgattggct
    10381 gcttattagg cagtccgggc tttcttgggt atttctttgg ggttggtttc tttttttgaa
    10441 taacgatgat atgccgttga tcagagacat tcggcaaggt aaccgcctct tctttgagga
    10501 atttaccacc taataaagca atcgcttgtt ttgcttctgt caattcctct tcactttttg
    10561 ctgctttcaa tgcataaaaa tagccttctt gtttagccaa gggcaaacac aattccgcta
    10621 acacattcaa acgagcaacc gcacgagcag tgacaaggtc aaatgatgca cgaaagtgag
    10681 gattttgccc aaatgtttct gcccggtcat gatataagga agcatcgatt cctaataatc
    10741 gggttaactc agataagaag gttattcgtt tattcagaga atcaacgatc gttatctgca
    10801 gttctggaaa ggcaattttt aaaggtatgc taggaaaccc tgcccccgaa ccaacatcgc
    10861 ataaagataa aggttgcgta aagtctactt caaatgctaa tgtaagtgaa tcgtaaaaat
    10921 gcttcagata tacgtctttt tcatccgtaa tagctgtaag gttcatcttt tcgttccatt
    10981 cgactaatgt ccgataataa atagcgaatt gattcatttg atgatcggta agctctatcc
    11041 ctttttctgc caaggattgg cgaaattctt ctggtatcat aaatattcct ttcattgtga
    11101 aacagcaatt gtttcacaga ttctctccct actttaacgc aaaatctcta gacatgcaat
    11161 ccttccatat ggattcttaa aaatttgaaa agtaagaaaa gccgatagac tttttctttc
    11221 tagtatgact cataaaatat acacagaaga gagaactagc tttgagcaat ttctgcaaaa
    11281 actagttctc tcttctattt attattacgt attacgctgg ttcactgact ttcgcaatct
    11341 tcccttgttc gatatagacc attaggatac ttaggtctgc agggtttacg ccactgattc
    11401 ggctagcttg ggcaattgtt tctggttgga ttttctgcaa tttttgtttt gcttctgttg
    11461 ctaatccgtt gattgctgcg tagtcgatgt tttctggaat ctttttcgct tccatgcgtt
    11521 ttaatttttc tactttttcc atggcttttt tgatgtagcc ttcatatttg atttggattt
    11581 ctacttgttc gatttctttt cgtgtcaatt cttcattttc tggaataaac cgtagaagtt
    11641 ctgcataagt aatctcagga cgtcttaaaa agtcgcttgc tagtacacca tcttttaatg
    11701 gtgccgcatt aatcgttgct aaaaagtctt ggacttcttc tgttggtttg attcgtacat
    11761 tcatcaaacg tttgatttct tgttcaactg cttctttttt cttcaaataa ttcttgtatt
    11821 gatcttcttt tactaagcct acacggtgac ccatttctgt taatcgcaaa tctgcattat
    11881 catgacgtaa aagcagacgg tattcagcac gtgaagtcag caaacgataa ggttcgttcg
    11941 ttccttttgt taccaaatca tcgatcatga caccgatgta accgtcactt cgtttgatca
    12001 ctaaaggttc ttttccctga actttcaaag ctgcattgat accggcaatc agcccttgtc
    12061 cagccgcttc ttcataaccg cttgtaccat ttgtttgtcc agctgtataa agattttcaa
    12121 cgactttagt ttctaatgtc ggacggagct ggtgtggctc tactacgtca tattcgatag
    12181 catatcctgt acgcatcatt tctacttttt ctaatccagc aatagaatga agcatgttga
    12241 cttgaacatc ctctggtaaa gaagtagata aaccttgaac atagacttct tccgtattta
    12301 agccttctgg ttccaaaaat aattgatgac gtggtttgtc tgcaaaacgc acgatcttat
    12361 cttcgatgga tggacaatag cgagcgccta ccccttcaac gatccctgta aacattgggg
    12421 cacgatgtaa gttgtctcga ataatttggt gagtcgtctc acctgtatag gtcagccagc
    12481 aagattcctg atctagttta taggcactat ctggtgtgct gaagctaaaa tgattcggct
    12541 ctttgtcacc aggctgctct tctgtaacag aatagtcgat cgtacttgat tttactcgag
    12601 gcggtgttcc tgttttgaac cgttcgatag caaagccaag ttcttttaga tgattcgcta
    12661 gtccaataga aggttgagag ttattcggac ctgatgaata tttcaattca ccgataatga
    12721 tttctccgcg aagtgctgtt ccggctgtaa taacaactgc tttggcccgg tagctcgctc
    12781 ctgtggacgt cactactcca tgacaaacac catcttcgac gatcagttct tcgacgattc
    12841 cttgtcttag cgtcaaattt ggttcttttt cgatcgtatg tttcatttct gttgaataag
    12901 catgtttgtc tgcttgtgct cgcaatgctc ggacagctgg gccttttcct gtgttcaaca
    12961 tacgcatttg gatataggtt ttatcgatat tcttacccat ttctccgcct aacgcatcga
    13021 tttctcgaac gacaacgcct ttagctggtc cgcctacaga aggattacat ggcatgaatg
    13081 ctaccatgtc caaatttatt gtcagtaata atgtttttga tcccatacgt gcagcagcaa
    13141 gtgcagcttc tgaaccggcg tgtcctgcgc caacaacgat cacgtcatac gcatctgctt
    13201 cataatgttc caatcgtttg ttcctccttt tattttccta aacaaaattg actgaataat
    13261 tgagtgatta gctcatcttg tacactgtcg ccgacaactt ctcctagata gtcccagcaa
    13321 cgcgtcatat ctatttgaac gagatccaca ggcattcctg aatcaatacc tgcaatgact
    13381 tcttcaagtg agagggaagc tttttctaac aacgcgatat ggcgggtatt agaaacataa
    13441 gtagcgtctc tttctcctgt ttctccgctg aagaataaat cagaaatagc tgattccaaa
    13501 cgatctagtc cgtcgttttt agctacggaa attgaaaaaa ctggttcatt ttcgatcaat
    13561 ttcttcaatt tttcttgttc cagctgtgct ggcaaatctg ttttatttag aagaatgatc
    13621 cgtttcaatc cacttgtcgc ttccaataat tgttcatctt ctgctgtcag tggttcgctt
    13681 tggtttagaa caagtaagat caagtctgct tctgctaatg cttttcggct tctttcgacc
    13741 ccgatccgtt ccaccacatc ttctgtttcc cgaattccag ctgtatcgat tagcttgagc
    13801 ggtacccccc gaacattgac atactcttcg attacatctc gagtcgttcc tgcaatatcc
    13861 gtaacgattg ctttttcttc tcttaacaaa tggtttaaca aacttgattt gccgacattt
    13921 ggccggccga tgattgccgt acttaaccct tcccgcagaa ccttcccttg cttggatgta
    13981 gcaagtagcg cttggattct ttgttggatc atctgcgctt tttcaagcaa taacttcgta
    14041 gtcaattctt cgacatcatc atattctgga taatcaatat ttacttctac ttgtgctaat
    14101 gtctctaaaa tctcttgtct caatgagcgg atcaaagcag acagattgcc gtctaactga
    14161 ttaagagcta gtcccattgc tttgtctgtt tttgcccgaa tcaaatccat cacagcttct
    14221 gcctgtgaaa ggtcaacacg accattcaag aatgctcgtt ttgtgaattc gcctggctca
    14281 gctaatcttg caccttctct taacaatagc tgcaaaattt gattgactac aacgatccca
    14341 ccgtgacaat tgatttctac tacatcttcc cgcgtaaatg tctttggtgc tctcatcacg
    14401 gagaccatga cttcatcgac aagctgattg ctcttgggat caacgatatg tccataatgg
    14461 atcgtatgac ttggtacttc tgataatcgt ttgtttccag attgataaac tttatctgct
    14521 agttgcacag cttgatcgcc acttaaacga acgatactga ttgcaccttc tccaggtggg
    14581 gtagaaattg ccgcaatcgt atcaaattct aaagtaattt cagccatttt ttcacttcct
    14641 ttttcatttt gcgcacaaaa aaagtgccca ctccaccctt tatgcacaat aaacggtgaa
    14701 cttcgcactt tgactgcttg ttcttgatta aattctggtc atagattatc acacttgcta
    14761 taaggaatca agtgtttaaa acaattagat gaagttgagc ctgttctgct tccattacct
    14821 tatatttaat tgtccttgga tgtctctttg cgttctttgg tttattcgaa gagaagatac
    14881 tgtataaaca aggctttatc tttcttaaaa aaaacggtac actaaatggt agcgctatct
    14941 aaactataca cttttgaaag gagaaaatat gtgagcccgt tcagatctgc tgcattggaa
    15001 gaagaaagtg accctcggat caacgatgtc gttttatcta cagggaatat caaccagaaa
    15061 tatatcgtcc gcgatatcgt ttttgttgtg gaatgcttag aagctgattt gtttgaccca
    15121 tcttttccca aagatgacct tttcttaaca acgaagagga aattaaagac gaaggtactt
    15181 gaatatggtg cgaatgcagt aatcaactgt cattttgagt atcagcaaaa aaaacgcgac
    15241 ggaaagtatt ttctggaact ttttgcttat ggaacagtga tccaatttat ccaaacaacg
    15301 attggttaaa tgaaacagac aaaaaaagcg gatcaaaact gaagacagtt tcgatccgct
    15361 tttttctatc aaagttagtg actacttcat cgtatactct gataagaatg atttctagta
    15421 aaatatactt ggtttgaaaa actttgtcaa cgtgatttat tcaaaaagta atggaatcaa
    15481 taatattttt tagcaggttc tactactaaa taacgataag gttcctcacc ttccgaatgt
    15541 gtttttacat gttcatcttt gcttaatgca gcatggattt gtttacgttc aaatgcaggc
    15601 attggttcta agaaaacagg ctgccctgtc cgttttactt tttcagccgt gcgatcagct
    15661 aaacgctgaa tgatctcttg gcgcttttca cggtaattgc ctacattgac tactacagac
    15721 agcttatttc ttgctaaacg atggatgaag acttgtgcca gatattgcaa tgcattcagt
    15781 gtttttccat gtttcccaat cagtatgcct tgcttttgtg tttctaaatg gaaaacaacc
    15841 atattatttg attctctttc cattttgaca agagctggag catcaagctg ttttgaaatt
    15901 tcagtaagat aaactgccaa ttccgttaaa gctgcttcat cacctagctc acttacttct
    15961 tctgtttttt cttcaagaat cgtttcgttg actacctcga tctcagctgt tggttcttct
    16021 tccaaaattt cttctacagt ctgttctact gtttcagaaa ctttttcgct gatacttggc
    16081 tggatcgaaa gccgtgcatt ctttcgaccc attcctagga aacctttttt tccttcttcc
    16141 agtatttcga tttcgacttg gtctttggcc aaacccaaat cagccagacc tagttggaca
    16201 gcttcgtcta ttgttgcagc ttcataaatc ggcattagca ttacctcccc tattatttcc
    16261 gttttttctt aggattcatt gctttcttaa gagcacgttc tctttcgcgg attctccgtg
    16321 cttcttcttc acgctctttt cgaattttga aagggttgtt gatcagcagc gtttgtacaa
    16381 cttggaaggc attggaaact acccagtata atgaaagacc gctggctaaa ttgacaccca
    16441 tcactaaaat cattacaggc atcacgaaat tcatgatctt cattgatgca ttcgattcaa
    16501 tttgactcat gctagataaa tacgtacttg caaaagtaaa gattgctgct aaaatcggca
    16561 aaataaatgt tggatccgcg ttccctaagt tcaaccataa gaaatgccct tgtttcaact
    16621 ctggtacacg tgaaatagat tgccataacg ccatcaagat tggcatttgt actaataaag
    16681 gaagacaacc agcatatgga ttgacgccat tttctgcata taaacgctgc tgctcttctc
    16741 ttaacttact ttgtgtttcc ggatctcttg aactatattg ttgctgcagt gcttttaatt
    16801 tcggttgtaa ttcttgcgtt ttacgcatgc tctttgtttg gaaatgcatc aacggcaata
    16861 ggatcacgcg gatgatcagt gtaaataaaa tgatccctat ccctgcatta ccaaatgaca
    16921 acgccttgat cgcctcagca aagtaataaa caatataacg atcccaaatt cctgtactct
    16981 gtgcgctgac ttccccagtt ccgcaaccgg ataagacgat gaccaaacta acgataccgg
    17041 ccatcaaaag taaacgtttg tatttcttca cttactcttt tcctctctgt cgattatttt
    17101 tgcaagtttt aagacgtgga ctaaattgga gcgaacctca tcataagtca accccttcac
    17161 acttggtcta gcaatcacaa taaaatccaa ctccggatca atttcttctt ttatagaata
    17221 aactgctgct cggatttttc gtttgacttc attgcggttt accgcgttgc cgaccttttt
    17281 tcctacagat aaaccaactc gaaaatgagg ctgtcccggt ttttccaaac ggtaaacgac
    17341 gaatttgcgg ttagcaaaag aatctcccgt atggaacacg gcttgaaact ctcgttcttt
    17401 ttttactcga tacgcttttc tcattagttt tctttccttt tctatctctc acttacgctc
    17461 ataatcatac cataattacg attgtccttt catcatttcc ctaaaaaaga gtataaaaaa
    17521 aaccactgaa accccagtgg cctaagctga aagaactttt cttcctttgc gacgacggct
    17581 agctaacaca cgacgtccat ttttagtact catacgttta cggaatccgt gaactttttg
    17641 acgtttacgt ttatttggtt ggtatgttct tttcatatat tccacctcca aaatgttctt
    17701 atctatttta taaacacaga catactttga tagtataacg acaaagtagt gattttgtca
    17761 atataaaata gaagatcgat tgttaaaact tatccacaag attatttttt tctatgggaa
    17821 tcctatgtgg aaaaagttat gcacagggat tatccacaga taataaaagg ctggttttac
    17881 acagaaaaca cacgtgtaaa aaaagtaatc cacaatcagg cgatttcctg tgtattccgt
    17941 ctcctaacta ttggtatatc tgtatttttt atccacagac gcaagaggaa atctattggt
    18001 ttttacgagt tttccacctg tgcattagtg gtgtggaaaa ctttttttga caactgtgtt
    18061 tttctctttt ttctttttgt cgattgtgtg gataattgta attaaaaaat ttttttcttc
    18121 ggtttctgtg gaaaactatt gaaaaaaatg ctaaattagt actagttatt cactttttaa
    18181 tgaaggagga ggccttatat ggtatccctc gatgctttat ggaatgaatt aaaagcaaca
    18241 taccaaaagg atttatcgcc tgctagctat aatacatgga tcgaaacagc gaaccctcga
    18301 acccttgatc aaaaccagct cgttgttgag gttcctagca agattcataa agaatattgg
    18361 gaaaaaaacc tggcgacaaa aatcgttgaa gtcggttata tgttatcagg aaatgagatt
    18421 atccctcgct ttattactgg cgaagaagca gagcaagaag aagttataga agaaaaaaat
    18481 ccaaaagttg tggcacctag tccgctaaaa aaagccatgc tgaaccctaa atataccttt
    18541 gatacgtttg tcattggtaa aggaaaccag atggctcatg cagcagcgct cgttgtagca
    18601 gaagatccag gttctattta taacccgctc ttcttttatg gaggcgtggg acttggtaag
    18661 actcacttga tgcatgcaat tggtcatcaa atgctgcaaa gtcagcctaa tgccaaagta
    18721 aaatatgtaa gcagtgaaac ttttgctaat gatttcatca attcgatcca aaacaaaaca
    18781 gcagaagagt tccgtcaaga atatcgaaat gttgatttac tgctagtgga tgacattcaa
    18841 ttttttgcag aaaaagaagc tacgcaagaa gagtttttcc atactttcaa tgccttgtac
    18901 aatgaaggga aacaaatcgt actaaccagt gatcggttgc caaacgagat tccgaaactg
    18961 caagaacgcc ttgtttctcg ctttgcatgg gggctgtctg ttgatattac accgccggat
    19021 cttgaaacaa gaacagcgat tttacgtaaa aaagcggcgg ctgaacgtct agagatccca
    19081 gatgacacac taagctacat cgcaggacaa attgattcta acattcgtga attagaaggt
    19141 gcgcttgttc gagtgcaagc ttttgctgcg atgaatagtg aagatatttc gactagtcta
    19201 gctgcagacg cactgaaaac gttaaaatca ggaaaaggac atcctcaatt gtcaatcctg
    19261 cagatccaag aagaagtggc aaagtattat cacatccaat tgaaagatct caaaggaaag
    19321 aaacgcgtca aatctattgt tgttcctaga caaatcgcta tgtatttggc acgtgagctc
    19381 acagataatt ctttaccgaa aatcggagct gaattcggtg gcaaggatca tacaacggtt
    19441 atccatgctc atgaaaaaat ccagcagctg atggattcta gtgtcagtat gcaaaatgaa
    19501 gtatcagaaa tcaaaaactt tttactaaat taatttgtcg attctatcaa ctgaaaatag
    19561 catgtggata aaagaaaaaa acgttcaaaa gttatacaca ctttttccac agctgttttt
    19621 tcgtttctct cttacgtttt ttgagttttc cacagaatca acatgcccta ttacttttat
    19681 tattcttttt aactaataat aaataaacaa acattcaaag gagatacatc atgaaagtta
    19741 ctttaaaccg agctagcttt atgcaggaat tgcaaactgt tcaacgagct atttcaagca
    19801 aaaccacgat ccctattttg acaggtgtaa aaatcacact gacacaagaa ggtttgactt
    19861 tgacggggag caacgctgat atatcaattg aaactttttt gtctgttgaa aacgaaaaag
    19921 caaatatgca aatcgaatct actggttcca ttgttttaca agcacgtttc tttagcgaaa
    19981 tcattcggag acttcctgaa gaaacattta ctttagaagt tttagaaaat aaacaagtag
    20041 cgatcacttc tggaaaagcg aattttatcg taaatggatt agatgcagat aactatcctc
    20101 atcttcctgt tgtcgaaagc cataaccaga tgaaattacc tgtacacgta ttgactaaac
    20161 taatcaacga aacagttttt gctgtctcac aacatgagag tcgtccaatc ttgacaggtg
    20221 tgcatttcat tttatctgat aattctttat tagctgtagc tactgattct caccgtctaa
    20281 gtcaacgcgt gattccagta gaacaagcgg ctgatcattt tgatattgtt attcctggaa
    20341 aaagtttgat cgaattatct cgctcattaa caaatgaaga agaaatcgtc gaaatcagca
    20401 ttatggaaaa ccaagtgttg ttcaaaacag aaacgatgta tttctattcc cgtttgctag
    20461 aaggaaacta tccagatacc aatcgtttga ttccttcaag ttttaataca gaggttgaat
    20521 tttctgttcc tagcttttta gcggcgatcg aacgggcttc tttactttct catgaaggcc
    20581 gtaacaacat cgttcgttta tctattcgtc cagatgcggt tgttttatac ggaaattcgc
    20641 cagaaatcgg gaaagtagaa gaaagcttga gttatacagc aagcagtggc gatccattag
    20701 atatttcttt caatccggat tatatgaaag cagcgttgcg tgcatttgga gatatgagca
    20761 tcaaagtgaa atttatctct gctattcgtc catttacatt ggaaccaacg gaagatggcg
    20821 ttcaatttat tcagctgatt actcctgtac gtacaaatta acaatgattt tcaatgttct
    20881 tgaaaattta attgaaaaga aatagaaaaa ataaaatttt aaacagaagg ccctaaaatc
    20941 tcttttaagg ctttctgttt atttttgttt aaatcacctt ttttagatta aatcgtctta
    21001 tatggaaaaa tatatggata aatttgtttt attgcgaaaa aaaaggtata atagagtata
    21061 cttttaataa tgaaaatgag ggatgggcat tgaaacaaca agtcattcta aatacggaat
    21121 tcatgacgtt gggccaaatg ttgaaagaag tcacagttat cggtagtggc ggacaagcaa
    21181 aatggtattt ggcagaaaac actgtgttag tcgatggcga accagaaaat cgtcgtggac
    21241 gcaaacttta tcccggcatg atggttgaag ttccagaagt aggaactttt tttatggtaa
    21301 aaaaaggaaa cgacgataat gaggctgaat aagctgtatt taaaaaatta tcgaaattac
    21361 gaagagctga acatcgaatt ttccaagaac ctcgtcatct ttttaggaga aaatgcgcaa
    21421 ggaaaaacga atcttttgga aagtatctac gtattagcta tgacgcgaag tcatcggaca
    21481 agtagtgagc aggaattgat tggctggact gacgatcagg cgatgattca aggagagatc
    21541 acaaaaggtt catccactct tccattagaa atcttattat ctaaaaaagg ccgaaaaacc
    21601 aagatcaacc atatcgaaca gaaaaagtta agtacttacg tgggacaaat gaatgtcatt
    21661 ttatttgcac cagaagatct ttctttagta aaaggcagcc cgcagaccag gcggaagttt
    21721 ttagatatgg aattaggcca gatcaatcca atctatcttt atgatcttgt ccaataccaa
    21781 gcaattttga aacaaagaaa ccagtacttg aagcaattga atgaaaaaaa acaaactgat
    21841 ttgctttatt tggatatttt gtccgaacaa ctagcagcgt ttggcagcaa agtattgaaa
    21901 gcaagagcac agttcgtcaa gcgattggag ttttgggcta attctttgca tcagcaaatc
    21961 acccatcaaa aagagcagct ggaaatcgag tatctcaccg cagtcgattc tttagaaaca
    22021 catacgcaag agcaaatcca agaacaattt ctcgcacttt tgaaccaaaa taaaaagaaa
    22081 gacctttttc ggggaacaac gacagttggt cctcatcgtg acgatctttc cttttttatt
    22141 aatcagaaaa atgtgcagac ttatggatcg caaggacagc aaagaacaac tgcactaagt
    22201 gtcaaattag cagaaatcga tttgattaaa gaagagacag gggaatatcc aatccttttg
    22261 ttagatgatg tgatgagcga gctggatgat aatcgtcaac tccatctgct agaaacaatc
    22321 gaaggaaaag ttcaaacgtt cttaacaaca acgacacttg accatgtaaa aaataaaatg
    22381 actgttgaac ctgagatttt ttatgttcaa caagggaaac taaaaggaga agaaactgaa
    22441 tgacagaaga aagaagttta gtagaacgcg ctaaagagta tgatgccagt cagattcaag
    22501 tacttgaagg acttgaagcc gttcgtaaac gacctggcat gtatatcggt tcgactagtt
    22561 ctgagggtct gcatcacctt gtttgggaaa ttgtcgataa ttcgatcgat gaagtattag
    22621 caggttttgc aacgaaaatc catgtgatta ttgaaaaaga caacagtatc accgttattg
    22681 acgacggtcg ggggatacct gtagatatcc aggcaaaaac tggacgtcct gcagttgaaa
    22741 cggtctttac tgttttacat gctggaggaa aattcggcgg tggtgggtac aaagtttctg
    22801 gtggacttca cggagtagga tcttctgttg ttaacgcgct ttctacacag cttgatgtca
    22861 aagtatacaa agaaggaaat gtgtactatc aagaatatcg tcgtggagct gtagtggacg
    22921 acttaaagat tatcgaacaa acggatcgcc atggaacaac ggttcacttt acgccagacc
    22981 ctgaaatttt tacagagaca actgagtttg atttttcaaa attggctacc cggatccgtg
    23041 aacttgcttt cttgaaccgc gggatgcgta tatcgattga agacaaacga gaagaagaac
    23101 cagtcatcaa tgaataccat tatgacggcg ggattaaaag ttatgtcgaa tacttgaatg
    23161 caaacaaaac cgtgattttt ccagaacctg tatatttgga aggtgaacag caagatatcg
    23221 cagttgaagt ctctatgcaa tatacagacg gctaccattc caatatcatg agttttgcca
    23281 acaatatcca tacgtatgaa ggcggaacac atgaatcagg attcaagact gctttaactc
    23341 gtgtgataaa tgattatgcc cgaaaacaaa agctgatgaa agaaaatgac gacaacttga
    23401 ccggtgaaga cgtgcgagaa ggattgacag ctgttatttc tatcaagcat cctgaccctc
    23461 aattcgaagg acaaacaaaa acaaaattgg gcaattccga agtacggaca gtcactgatc
    23521 ggttatttag cgaacatttc atgaaattct tgctggaaaa cccaagtgtg ggccgtcaaa
    23581 ttgttgaaaa aggattacta gcttccagag ctcgtctagc agcgaaacgt gcgcgtgaag
    23641 taacgagacg taaaggtgct ttagagatta gtaatctgcc aggaaaatta gcggactgct
    23701 ctagcaacga tcctgaaaaa tgcgaattgt tcatcgtcga aggagattcg gccggcggat
    23761 cagctaagca aggtcgtaat cgagagtttc aagccatctt gcctattcgt gggaaaatct
    23821 tgaatgttga aaaagcaagc atggacaaga tcttggcaaa tgaagagatc cggtctttat
    23881 ttactgctat gggtacaggg tttggagaag attttgatgt atcgaaagct cgctaccaca
    23941 aattagttat catgactgat gccgatgtcg acggcgcgca tatccgtaca ttgctgctga
    24001 ctctttttta tcgttacatg cgtcctattg ttgaagctgg atacgtctat attgctcagc
    24061 cacctttata cggggtaaaa caagggaaga atattacgta tgtacaacca ggaaaaaatg
    24121 cagaagaaga gttgaaacaa gtcgttgctt cacttcctgc ctcaccaaaa ccaagcgttc
    24181 agcgttataa aggacttgga gaaatggatg atcatcaact atgggaaaca actatggacc
    24241 caagcaatcg gatgatggcc cgcgtcagtg tggacgatgc tattgcagca gatcagatat
    24301 ttgaaatgtt gatgggggat cgagtagaac cccgtcgtgc attcatcgag gaaaatgcac
    24361 actatgtgaa gaacttagat atttaatcat gaaggaggaa aaagaatgag tgaagaaatc
    24421 agagaaaaca tccatgatgt caatctaacc agcgaaatga aagactcctt catcgattat
    24481 gcgatgagtg ttattgtcgc acgtgcactt cctgatgtca gagatggtct gaaacccgtt
    24541 catcgccgta ttttatacgg catgaacgaa ttaggtgtaa caccggataa agcccataaa
    24601 aaatctgcca gaatcgtcgg agatgtcatg ggtaaatacc atcctcatgg agactatgcg
    24661 atctatgagt caatggtacg gatggcacag ccattcagct accgttatat gctagtggat
    24721 ggtcatggaa actttggttc tgttgacggt gacggtgccg ccgctatgcg ttataccgaa
    24781 gcacggatga gcaaaatcgc tacagaaatg ctgcgtgata tcaataagaa cactgttgat
    24841 ttccaaagca actatgatga tacagaaaaa gaaccagttg tactacctgc tcgcttcccg
    24901 aatcttttag tgaacggaac aacggggatc gccgtaggta tggccacgaa tatcccacct
    24961 cataacttgt ctgaggtagt agcggctatc gatttgctga tggataatcc tgatgtgacg
    25021 accaatgaat tgatggaagt attacctgga ccagattttc caacaggtgg tttggttatg
    25081 ggaaaatcag gaatccgaag agcgtatgaa acaggtaagg gttctattac cgtacgtgca
    25141 aaagttgaga ttactgagat gccaaatggt aaagaacgga tcatcgtgac agaattgcct
    25201 tatatggtca ataaagcaaa attgatcgaa cggatttctg aactgcacag agacaagcgt
    25261 atcgaaggga tcacagattt gcgggacgaa tcttcccgtg aaggtatgcg gatcgtcatt
    25321 gacatccgtc gtgacgccag cgcatctgtt atcttgaata acttatataa attgacatcc
    25381 ttgcaaaatt catttggatt caatatgttg gcgatcgaaa aaggtgtgcc gaaagttttg
    25441 agcttgaaac aaattcttga aaactatatc gaacatcagc gcgaagtcat cacaagacgg
    25501 acagtctttg aaaaagaaaa agcagaagca agggcacaca ttttagaagg tttgcgtatt
    25561 gctttggatc atatcgatga aatcattgcg attatccgcg ggtctaaatc agatgacgaa
    25621 gcaaaagcga cgatgatcga acggttcgat ttatccgatc gccagtctca agctatttta
    25681 gatatgcgtt tacgccgatt gaccggtttg gaacgggaaa aaatcgaaaa tgaataccaa
    25741 gaactcttaa aactgattga agatttaact gacattctgg caagaccaga acgtgttaca
    25801 gaaatcatca agaatgaatt agcagacttg aatcaacgat ttggtgataa acgacgcaca
    25861 gaattactag tcggtgaggt cttaagtctg gaagatgaag atttaatcga agaagaagaa
    25921 atcgttatta cattgacaaa caatggttac atcaaacgtt tggcaaataa tgaattcaga
    25981 gctcaacgaa ggggtggacg tggtgtccaa ggaatgggta tccacaatga cgacttcgtc
    26041 aaaaatctgg tttcttgttc tacccatgat acattgttat tctttacgaa taacggaaaa
    26101 gtgtatcggg caaaaggata cgaaattcca gaatacgggc gcacagccaa aggaatacca
    26161 attatcaatt tactaggtat cgattcgagt gaaagcatcc aggcaatcat tgcggtgacg
    26221 ggagaagcag aagaaggcca ctatctcttc tttacgacgc gtcaaggtac agtaaaacgt
    26281 acttctgtca ctgcttttgc taacatccga agtaatggtc tgatcgcaat tggtttgaaa
    26341 gaagacgatg agctcgtcaa tgtgctactg acagatggtc aatctaatat cattattgga
    26401 acacataacg gctactctgt cactttcaaa gaagaagcag tccgcgatat gggacgtaca
    26461 gcttccgggg ttagaggaat acgcctgcgt gaggaagatt atgtcgttgg ggcagcctta
    26521 atgaaggaag atcaagaagt attagtgatc acagaaaaag gttacggaaa acggacaaaa
    26581 gtatcggaat atccagtgaa aggccgcgga ggtaaaggga tcaaaacggc gaacatcact
    26641 gaaaaaaatg gtccactagc tggattgacc actgtttctg gagatgaaga catcatgctg
    26701 attacagata aaggtgtcat tatccgtttc aacgtatcga ctgtttctca aactggacgt
    26761 tcaacattag gcgtacgttt gatgaagatg gaagctgata cgaaagttgt cacgatggca
    26821 gcagtcgaac cagaagctgc agaacaaaca gttgaagaac aaggatcaga agaatagcca
    26881 tcaagactaa ttgtgtaagc tagcaagaat gaccgattaa aaaatctggt tattcctgct
    26941 ggcttttttg attagttgaa tttctcttaa aatattcgaa actgttcatt ctgtattgct
    27001 tttgaccaaa atagagtgta taatcagagc ttgtgagtag tctaattaat tagataactc
    27061 tccttgctct taggaaccta agagccgaaa gtccacaggg aggtgaatat caatggaaaa
    27121 cacgaaatat gaaatcatgt acattattcg tccaaacatt gatgaagaag caaaaactgc
    27181 tttaatcgaa cgtttcgatg caatcttgaa agataacgga gcagaagtta tcgaatcaaa
    27241 agattgggaa aaacgccgtt tcgcttatga aatgaacggt taccgtgaag gtatctatca
    27301 catcgttaaa gtatcttctc catcatcagc aaatgctgtc aatgaatttg accgtcttgc
    27361 taaaatcaac gacgatatca ttcgtcacat gattgttaaa gaagaagctt aattgttgtt
    27421 tcatgtgaaa caatagaaaa cgatgagagg agatgactat tttgattaat aatgttgtat
    27481 tagttggacg tctgacgaaa gatccagatt tgcgttacac agcaagtggt acggcagtcg
    27541 caacatttac tttggctgtg aatcgtaatt ttacaaacca aagcggcaat cgcgaggctg
    27601 attttatcaa ctgtgtgatt tggcgcaaat cagcagaaac tttagcaaac tatgctcgta
    27661 aaggaacact tttaggtgtt actggacgta tccaaactcg ttcttatgat aatcagcaag
    27721 gacaacgtgt atacgtgaca gaagtagtag ctgacaactt ccaattgctg gaaagtcgtt
    27781 ctgcatctga aaatcgccaa caaagcggcg gattccaaag ttctggtcaa tcagctggcg
    27841 gatttggcgg aaacaataac tctaatcaaa catctcaatc atccaacggt atgcctgatt
    27901 tcgaccgtga tacttctgac ccattcggct caagttcaac gatcgacatt tcagacgatg
    27961 atttaccatt ctaaacgaat ttgataggag ggaaatagaa tggcacaaca aagaagaggc
    28021 ggacgtaaac gtcgtaaagt agactacatt gctgctaacc atattgaata tatcgattat
    28081 aaagatatcg aattactaaa aagatttatt tcagaacgcg gtaaaatctt gccacgtcgt
    28141 gtaactggta caggcgctaa aaaccaacgt aaaattactg ttgcaatcaa acgcgctcgt
    28201 atcatgggct tgctaccttt cgttagcgaa gactaataaa tcctaggaat tgaatgactg
    28261 atccttttga tcagctattc aattcctttt tttctgtaaa aataacctct attcgtttag
    28321 tcgctcgaat gacgaaagtt atggtaaaat caaattagtc atatatggga ggaaaattat
    28381 ggaaaataaa aacaatcatt tatccccgaa acctctcttc cttatcctgt ttatattaat
    28441 cgaagtattg gtagtcttaa ttttaccatt tcgttttgtt agcgcttcgg tcattctatt
    28501 gatgaattta tggggtgttc gctattggtt gaaaatgaaa cgttggctta ttttaaataa
    28561 acgtgaaaaa atccagcagg cttcgactgt agctgaaacc aatctagctt atgtctcaga
    28621 agtcatgcct gtaggagtga ttgcttatca gccatcaagc caaaaaatcg aatggctcaa
    28681 tccattagca ttgaaaatca aagatcaaag tggattaaca gaggaagaat tgctagatac
    28741 tttttttact gcaaaagaaa aaaagaaaaa ccaagtctat ctagcagata aaacatttca
    28801 gtttgatatg gatacagtaa agggtgttgt ttattttaat gatattaccc aaaaccagtt
    28861 gttaaaactc catcagacga acagtcagcc ggtgattggt attttatcta ttgataacta
    28921 tgcagatgcc attgataaat tggatgacaa agagatttct tatttgaact ctttcgtcac
    28981 tacactagtt tctgactgga tgaatgaata ccatgtcttt ttcaaaagaa tcaacgcaga
    29041 acggtttttc ttcgtagcac agatgatcga tctgaaaaag atgatggata aaaaatttga
    29101 tttgctggat cgctttcgag cagaagctga aaaacagagc tcgcctatca cccttagtat
    29161 ggggattgct tatggctcaa gaaccctcga tgaaatcggt ggagaagctc agaataattt
    29221 agatgttgct ttagtccgtg gaggcgacca agttgtcgtg aaagaggtaa aagaaggagc
    29281 aaaacctgtt tattatgggg ggaaatctgc taagaatgtc aaacgcacgc gtgttcgttc
    29341 tcgagcgatg agtaccgctt tgcgtcggat ttttgatgaa acaggcgatg tgtttatcat
    29401 ggggcacaag tttccggata tggatgcttt aggctctgct tttggtgtca gttgtctggc
    29461 acgcttcaac cagaaaaaag cttacatcgt tatcaacgag aacgagatca ttccagatgt
    29521 agaacggtgt cttgaagaaa tccataagca tcctgaatta gaaatgcaac taatctctcc
    29581 agaacgtgcc atagagctga aaaaagaaga ggacctcctc gtgatggtgg attaccataa
    29641 accttctcta tcgatctctc agggcctata tgagcaattt gataagattg tgatcattga
    29701 tcaccatcgt cgtggggaag agtttccaag taaacctctg ctgacttata tcgaatcttc
    29761 agcttcttca gcttcagagc tagtttctga gctgatccaa taccaaagca gcaaaacaaa
    29821 tcgcttagat cgttttgagg ccacattatt gcttgcaggg atggttgtag atacaaatag
    29881 tttcaatgtc cgtacaactg cacgaacttt tgacgttgcc agctatttaa ggtcttgtgg
    29941 agcagacgca tccttagtac aatatttatt gagctcggat ttagcgacat acttggagat
    30001 cagcaatttg attgctggaa gcgactatgt gacttctgat attgttgtgg cggcagggac
    30061 agacgaaaaa gagtataata gtgttatagc agcaaaaaca gcagatacat tgctttcgat
    30121 gatcaatatc aatgcagctt tcgtcattac aaagcggaca gacggcttga ttgggatcag
    30181 tgccagaagt accggcacga tcaacgtcca aatcatcatg gaatccttag gtggcggagg
    30241 acacttcaca aacgcagcta ctcaactgga aaatacgaca gttgaggaaa caaaacagaa
    30301 gctatttgct gcaatcaaag aaaatatgaa tcaaatatat gataaggagt aatccaaatg
    30361 aaagtaattt ttttacaaga tgtaaaagga aaaggtaaaa aaggggacat caaagaagtt
    30421 ccagcaggtt atgcacaaaa ttatctaatc aaaaataact tggcaaaaga agcaacgaaa
    30481 agcagtatca gtcagttagc aggacaaaag aaagcacaag aaaaacacga agctgaaatc
    30541 ttggaagaag ctaaaaagtt aaaagaattc ttggaaaaag aagatactgt agtcgaattg
    30601 aaggcgaaag caggggaaga cagccgtttg tttggttcga tcccttcaaa acaaattgct
    30661 gaagggttaa acaaacaata caaaatcaaa ttagataaac gaaaaatcga attagaaaat
    30721 ccgatccgtt cactaggcta cacaaacgta cctgttaagc ttcatcatga ggtgacggct
    30781 acgatcaaag ttcatgtcgt acaagaataa caaatcttga tagcgatgtt cagctggtaa
    30841 gtataaccag aaggaaaaaa gacaaaccga aatttatctg agaaaacagc tgcagcaatg
    30901 aaagcaaaag ctgcaaatcg atttcagatt gatttcggtt tcttttttgg gaacagcaaa
    30961 atagcataaa gaaaacgagt ataataaaag agaactattt ttttattaca ctttcggtac
    31021 gaatgatgac ttttctctca ttttcttgta aaatgaagca attgaagaca aggagcgata
    31081 cgtatgaatg aagtgtggca ggatcgaatc ccaccgcaaa atattgaagc tgaacaagcg
    31141 gttttaggct ctgtttttct tgacgcagaa acgatcattg atgccatgga gtacatagag
    31201 ccaaaagact tttatcgtcg tggccatcaa attatttttg agacaatgat cgagttaaat
    31261 gatcgtaatg aagcgattga cgtggttact gtcaaagacc ggctggaaca agccaatttg
    31321 ttagaagatg ccggtggttt aagttatctc tcagacttgg cattagcagt tccgacagca
    31381 gcaaatatcg tctactatgc gaaaatcgtt gaagaaaaat cattgctgcg tacattgatc
    31441 cagacagcaa caggaatcgt tacgaaagga ttcgagcaag gagaagaagt tcaaacaatt
    31501 ttagatgatg ccgaacgcag tatcttggag gtttcggaaa aaagaaatcg aagcggcttt
    31561 ttatctattg ctgaagtact gaactccacg attgaaaata tcgatcgact agctcaaaac
    31621 aatgaagaaa tcacaggtct accaactggc tatcaaggtc tggataaaat gacagcaggt
    31681 ttccagcccg aagaactaat cattcttgct gcacgtccag cggtaggtaa aacagcgttt
    31741 gcattgaata ttgctcaaaa tgtaggaaca aaaacagatc gttctgttgc gatatttagt
    31801 ttggaaatgg gggcagaatc tcttgtaaat cggatgcttt gtgcagaagg ttcgattgag
    31861 gccagccatt tacgtacagg tcagctttcg gaagaagaat ggtcgaatct gatcgttgcg
    31921 atggggagtc tttctcgagc aaatattttt atcgatgata caccagggat caaaatctct
    31981 gaaatccgtg cgaaatgtcg gaaactagcg caagaaaaag gcaatctagg cttgatattg
    32041 atcgattatt tgcagttgat tgaaggtaac ggtaaagaga accgtcaaca agaagtatca
    32101 gatatctctc gccaactgaa aaaactggcg aaagagctga aagtacctgt cattgctctt
    32161 tcacagttat ctcgtggagt agaacagcga caagacaaac gaccagtgct aagtgatatt
    32221 cgtgaatccg gatcaattga acaagatgcg gacatcgtag ccttcttgta tcgagatgat
    32281 tattatgaaa gagaaggcgg agatgacgat gatggaaacg agccaaaaaa tgacaacgtc
    32341 atcgaagtca tcatcgagaa gaaccgaagc ggtgcacgag gaacagttga actactgttt
    32401 atcaaagaat acaataagtt ttcgtcgctt tcgccaagag aagaatttta aagaagaggt
    32461 tgtgagcctt gagcaatccc gagagctctc gtaagaagat gcaaggagta accgcaggaa
    32521 cgactagagt acttaattta aacagatctc caaagggatt gtgacaaaag tcttgtcaca
    32581 gtcccttttt ccgaataaac ggtgttcaat cagctgaccc ttggtattag ctcagcaaac
    32641 gtcaaaatat gaggaactat tttgacgttt gtttctctaa tactcaggtc aaaacgctga
    32701 tcttacgacc tcttttcctt aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca
    32761 atatcatcaa acatgagaag cagttttttg aaattgctcc ttgtgactca gagttaaacg
    32821 gcaggctcac aacctctttc ttaaacggtg ttcaaaagct tgcatctgcg gtaatacgcc
    32881 caatcaaacg aaaaatatga gaagctattt ttgtttggct gttcttatta cttgatgcag
    32941 gacagctttt tcacaacctc tgattcacgg tgttccaaaa gtagcttctt cgaaaaataa
    33001 gtcgaaattt cacaaaaatt tgagaagcaa ttttcgtaca ttccttctta tttcttgaag
    33061 ctgaccactt ctgttacaac ctctttttta ataaccattc taaaaagata aagttcgttt
    33121 tttaagcgtt tttataaaat aaaaataaaa atcattcgat tttatttgaa atatgtatct
    33181 attattgata caatggttca ggattaaata atatagaacg aggtgttcac atgtcatctg
    33241 tagtagtagt aggtacgcaa tggggcgatg aagggaaagg aaaaatcaca gactttctca
    33301 gtgaaaatgc tgaagtgatt gcacggtacc aaggtggcga caatgcaggg cacacaatta
    33361 agtttaacgg tgtgacttat aaactgcatt tgattccttc tgggattttt tataaagaca
    33421 agatcagtgt gatcggaaac ggcgttgtag tcaatcctaa atcattggtt aaagaattag
    33481 cttatttaca tgaaaaaggt gtagaaacaa gtaatttgcg tatttctgac cgcgcgcacg
    33541 taatcttgcc ttatcatatc aaattggacc aattacaaga agatgctaag ggcgaaaata
    33601 agattggtac aacgattaaa ggaatcggac ctgcctatat ggataaagca gctcgtgtag
    33661 gtatccgtat tgcagactta ttagataaag aaatttttga agaacgtctt cgtttgaatc
    33721 tagatgacaa aaaccgtcaa tttgtaaaaa tgtttgattc agaaccactt gattttgacg
    33781 atatttttga agaatattat gaatacggac aacaaatcaa acaatacgtc acagatactt
    33841 ccgttatctt gaatgatgct ttggatgcag gcaaacgcgt gttgtttgaa ggcgcacaag
    33901 gtgtaatgct ggatatcgac cagggaactt atccttttgt tacttcttct aacccagtag
    33961 ccggtggggt aacaatcgga agcggtgtag ggccttcaaa aatcaataaa gttgtcggcg
    34021 tctgcaaagc ctatacgtca cgtgtcggtg atggaccatt tccaactgaa ttatttgatg
    34081 aaacaggaga acaaatccgc gaagtaggac gagaatacgg tacgacaact ggtcgtccgc
    34141 gtcgtgtagg ctggtttgat tctgtagtta tgcgccattc taaacgagtt tcaggtatta
    34201 ccaacctatc tttaaactcg atcgatgtat taagtggatt ggaaacagta aaaatttgta
    34261 cggcttatga attagatggc gaactaatct accactatcc agcaagcttg aaagagttga
    34321 accgctgcaa accaatctat gaagaattgc caggctggtc agaagacatt acaggatgca
    34381 aaacacttgc agaactgcca gaaaacgcac gtaactatgt acgtcgtatc tcagagcttg
    34441 ttggtgtacg tatctcaaca ttttctgtcg gacctgatag aacacaaaca aacatcttag
    34501 aaagcgtctg ggcgcaaatc taaggataaa aacgacgact gaatcaggac gttcttacca
    34561 gagcgagtcg aagtgtttat ctatacgata ataataactt gaacactgtt tattcagaat
    34621 aagggactgt gacaaaaacc gtgtcacagt ccctttttcc gaa
//
LOCUS       DARBFO010000019        34547 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000019 DARBFO010000000
VERSION     DARBFO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.020"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            212..604
                     /locus_tag="SCK51_001065"
     CDS             212..604
                     /locus_tag="SCK51_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1251707.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="SCK51_001066"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="SCK51_001066"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEG1251708.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="SCK51_001067"
     CDS             3165..4775
                     /locus_tag="SCK51_001067"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEG1251709.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="SCK51_001068"
     CDS             5067..5936
                     /locus_tag="SCK51_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEG1251710.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="SCK51_001069"
     CDS             6101..7372
                     /locus_tag="SCK51_001069"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEG1251711.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="SCK51_001070"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="SCK51_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEG1251712.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="SCK51_001071"
     CDS             8763..9026
                     /locus_tag="SCK51_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEG1251713.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="SCK51_001072"
     CDS             9471..9920
                     /locus_tag="SCK51_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1251714.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="SCK51_001073"
     CDS             9977..11395
                     /locus_tag="SCK51_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1251715.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="SCK51_001074"
     CDS             11395..12255
                     /locus_tag="SCK51_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEG1251716.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="SCK51_001075"
     CDS             12429..13202
                     /locus_tag="SCK51_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1251717.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="SCK51_001076"
     CDS             13349..13714
                     /locus_tag="SCK51_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEG1251718.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="SCK51_001077"
     CDS             complement(13862..15070)
                     /locus_tag="SCK51_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1251719.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="SCK51_001078"
     CDS             complement(15089..15367)
                     /locus_tag="SCK51_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251720.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="SCK51_001079"
     CDS             15521..16216
                     /locus_tag="SCK51_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG1251721.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="SCK51_001080"
     CDS             complement(16658..17455)
                     /locus_tag="SCK51_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEG1251722.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="SCK51_001081"
     CDS             complement(17452..18636)
                     /locus_tag="SCK51_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEG1251723.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="SCK51_001082"
     CDS             complement(18661..19908)
                     /locus_tag="SCK51_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEG1251724.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="SCK51_001083"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="SCK51_001083"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEG1251725.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="SCK51_001084"
     CDS             21888..22202
                     /locus_tag="SCK51_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEG1251726.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="SCK51_001085"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="SCK51_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEG1251727.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="SCK51_001086"
     CDS             22887..23531
                     /locus_tag="SCK51_001086"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEG1251728.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="SCK51_001087"
     CDS             23546..23875
                     /locus_tag="SCK51_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEG1251729.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="SCK51_001088"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="SCK51_001088"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEG1251730.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="SCK51_001089"
     CDS             24863..25687
                     /locus_tag="SCK51_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEG1251731.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="SCK51_001090"
     CDS             25680..26027
                     /locus_tag="SCK51_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEG1251732.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="SCK51_001091"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="SCK51_001091"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEG1251733.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="SCK51_001092"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="SCK51_001092"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEG1251734.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="SCK51_001093"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="SCK51_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEG1251735.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="SCK51_001094"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="SCK51_001094"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEG1251736.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="SCK51_001095"
     CDS             31657..32451
                     /locus_tag="SCK51_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1251737.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="SCK51_001096"
     CDS             32441..34483
                     /locus_tag="SCK51_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1251738.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11111 a         6123 c         7253 g        10060 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataaga
//
LOCUS       DARBFO010000020        32417 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000020 DARBFO010000000
VERSION     DARBFO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.021"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            34..411
                     /locus_tag="SCK51_001097"
     CDS             34..411
                     /locus_tag="SCK51_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251739.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            complement(591..1886)
                     /gene="purB"
                     /locus_tag="SCK51_001098"
     CDS             complement(591..1886)
                     /gene="purB"
                     /locus_tag="SCK51_001098"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEG1251740.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1899..3023)
                     /gene="purK"
                     /locus_tag="SCK51_001099"
     CDS             complement(1899..3023)
                     /gene="purK"
                     /locus_tag="SCK51_001099"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEG1251741.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(3016..3504)
                     /gene="purE"
                     /locus_tag="SCK51_001100"
     CDS             complement(3016..3504)
                     /gene="purE"
                     /locus_tag="SCK51_001100"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEG1251742.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3797..5104)
                     /locus_tag="SCK51_001101"
     CDS             complement(3797..5104)
                     /locus_tag="SCK51_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEG1251743.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(5107..5688)
                     /locus_tag="SCK51_001102"
     CDS             complement(5107..5688)
                     /locus_tag="SCK51_001102"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEG1251744.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5804..5901)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(6260..7552)
                     /locus_tag="SCK51_001103"
     CDS             complement(6260..7552)
                     /locus_tag="SCK51_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1251745.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7549..8172)
                     /locus_tag="SCK51_001104"
     CDS             complement(7549..8172)
                     /locus_tag="SCK51_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251746.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(8175..9011)
                     /locus_tag="SCK51_001105"
     CDS             complement(8175..9011)
                     /locus_tag="SCK51_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1251747.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(9028..9915)
                     /locus_tag="SCK51_001106"
     CDS             complement(9028..9915)
                     /locus_tag="SCK51_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1251748.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9923..11158)
                     /locus_tag="SCK51_001107"
                     /pseudo
     CDS             complement(9923..11158)
                     /locus_tag="SCK51_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(11841..12524)
                     /locus_tag="SCK51_001108"
     CDS             complement(11841..12524)
                     /locus_tag="SCK51_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEG1251749.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12542..13159)
                     /locus_tag="SCK51_001109"
     CDS             complement(12542..13159)
                     /locus_tag="SCK51_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG1251750.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(13169..13993)
                     /locus_tag="SCK51_001110"
     CDS             complement(13169..13993)
                     /locus_tag="SCK51_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEG1251751.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13986..14546)
                     /locus_tag="SCK51_001111"
     CDS             complement(13986..14546)
                     /locus_tag="SCK51_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251752.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14720..15676
                     /locus_tag="SCK51_001112"
     CDS             14720..15676
                     /locus_tag="SCK51_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1251753.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15981..16937)
                     /locus_tag="SCK51_001113"
     CDS             complement(15981..16937)
                     /locus_tag="SCK51_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEG1251754.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            17041..17643
                     /locus_tag="SCK51_001114"
     CDS             17041..17643
                     /locus_tag="SCK51_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1251755.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17731..19230)
                     /locus_tag="SCK51_001115"
     CDS             complement(17731..19230)
                     /locus_tag="SCK51_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEG1251756.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(19246..19770)
                     /locus_tag="SCK51_001116"
     CDS             complement(19246..19770)
                     /locus_tag="SCK51_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEG1251757.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            20068..20817
                     /locus_tag="SCK51_001117"
     CDS             20068..20817
                     /locus_tag="SCK51_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG1251758.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20808..21593
                     /locus_tag="SCK51_001118"
     CDS             20808..21593
                     /locus_tag="SCK51_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEG1251759.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21562..22278
                     /locus_tag="SCK51_001119"
     CDS             21562..22278
                     /locus_tag="SCK51_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG1251760.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(22380..23414)
                     /locus_tag="SCK51_001120"
     CDS             complement(22380..23414)
                     /locus_tag="SCK51_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEG1251761.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(23418..24476)
                     /locus_tag="SCK51_001121"
     CDS             complement(23418..24476)
                     /locus_tag="SCK51_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEG1251762.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(24696..25844)
                     /gene="nagA"
                     /locus_tag="SCK51_001122"
     CDS             complement(24696..25844)
                     /gene="nagA"
                     /locus_tag="SCK51_001122"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEG1251763.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(25920..26756)
                     /locus_tag="SCK51_001123"
     CDS             complement(25920..26756)
                     /locus_tag="SCK51_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1251764.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(26940..28157)
                     /locus_tag="SCK51_001124"
     CDS             complement(26940..28157)
                     /locus_tag="SCK51_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1251765.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(28306..28485)
                     /locus_tag="SCK51_001125"
     CDS             complement(28306..28485)
                     /locus_tag="SCK51_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251766.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            28540..28623
                     /locus_tag="SCK51_001126"
     tRNA            28540..28623
                     /locus_tag="SCK51_001126"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28574..28576,aa:Leu,seq:gag)
     gene            complement(28863..30233)
                     /locus_tag="SCK51_001127"
     CDS             complement(28863..30233)
                     /locus_tag="SCK51_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251767.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(30947..31237)
                     /locus_tag="SCK51_001128"
     CDS             complement(30947..31237)
                     /locus_tag="SCK51_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251768.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(31445..32137)
                     /locus_tag="SCK51_001129"
     CDS             complement(31445..32137)
                     /locus_tag="SCK51_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG1251769.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         9709 a         6813 c         5485 g        10410 t
ORIGIN      
        1 atttggataa tgggttcgag gaggcacttg ccgatggaag atgcaataaa acaaggtcag
       61 ctttttttat cagaattaac agctgaagaa cgttcctgtc tatataatga tttgctgtcg
      121 atcaaagacg tttatttatg caataaagat actagttttt atacattccg aaaaatccgc
      181 cgagcaacca agcgctgtgc tgaatgccgt aaaaggactt ttctttatca gacagaacac
      241 aaaggcgaac tttcaatcag tcagattcat gaacagaagc gtttcaaaaa tcattatcgt
      301 atccagatct tattcaatgg acaagagatg gaatacgctt ttctcgaaaa cttgctgcaa
      361 catctccata accagcattt tagttataat agccaagcca tttacgctta attcctctat
      421 tttttgtatg taaagaggca cggtaaagag gtagccgctt ttaaaatcag gttcctatta
      481 ttgataagaa aatataataa actaaaagaa gcagaaccgc taacggatct gcttcttttt
      541 tggttatttt tgctattcta ttttaattga attttgcaac tcactcttct ttattccaaa
      601 cctactcgtt tgaatatcac gtcaacgttt ttcaaatgat gatgataatc aaaggcatcg
      661 tctatttctt ctttagataa taccgatgtg atggttggat cttcttctaa cagcgggcgg
      721 aactgcactt ggttgtccca agcgtacgca gtttttggct gaacaagatc ataagcttct
      781 tctcttgtca ttcccttatc gatcaatttc agcataacgc gctggctata aatcaaacca
      841 aatgttgcag acatattccg cttcatgttt tcaggaaaga ccgttagatt tttaacgata
      901 ttgccaaaac gatttaacat gtaattcaat aaaatcgtgg tatcaggcaa gatgattcgt
      961 tctgctgaag aatgagaaat atctctttca tgccataatg tgacattttc ataggcagtg
     1021 accatgtgtc cgcgcaccac tctggctaga ccagccatat tttctgaacc gatcggattc
     1081 cttttgtgcg gcatagcaga agaacctttt tgacctttcg caaaaaattc ttctacttca
     1141 cgtgtttcgg atttttgcaa tccgcgtatc tctgttgcaa aacgttcaat acttgtagca
     1201 ataagcgcca tcgctgagaa atattcagca tgtagatccc gagggagaac ttgagtagag
     1261 atttcttgtg gacggatccc caattgttta catacatact cttcaataaa tggcgggata
     1321 tttgcgaacg ttcccactgc tcctgagatt tttcctgctt ctactccttt tgccgcatgt
     1381 tcaaatcgtt cgatgttccg tttcatttct gaataccatg tggcaagttt caatccaaaa
     1441 gtagttggtt cagcatggac accgtgtgtc cgacccatca tcactgtata tttgtgttct
     1501 tttgcttttt cgccaatgat ttccgtaaaa cgtttcaagt cttcccgtaa aatatcattt
     1561 gcttgcttga tgagatagcc ataagcagta tctacgacat ccgtacttgt tagaccataa
     1621 tggacccatt ttctttcttc tcccaatgtt tcagaaaccg ctcgtgtaaa cgcaacgaca
     1681 tcatggcgtg ttaatttttc gatttccaag atccgatcga tatcaaatga tgcatgctca
     1741 cgaatttttt tcacatcttc ttgcgggatt tcaccaagtt ctgcccaagc ttcatctgct
     1801 aaaatctcga cttcgagcca cgcacggtat ttgttctctt ccgtccagat attccccatt
     1861 tcagggcgtg tataacgttc taacatgttt tttctcctct aatcccaaat attcgtttgt
     1921 ttgatttctt ccagcgtttg ctggatgtct tctgtcaaga tagtgatatg tcccatcttg
     1981 cgaccttttt ttgcttgttt ttttccatag aaatggaatt gccagtctgg cttattctca
     2041 atcaagcgat agctttccag cagttgttct cccaaaacat tgaccattac tgcattgctt
     2101 atcaaacgta ctttttctcc taatggccaa ccacaaatac ctcgaatatg ggcatcgaac
     2161 tgactcatag aacaagcttc aatagaataa tgtccagaat tgtgaggacg aggagctaat
     2221 tcattcacat atagcccgcc tgtttttgtt aaaaacattt ctacccctaa aacacctgaa
     2281 aggctcactg cttctgcaat cacacgggca atacgctcag cctcctcgat cacatcctga
     2341 tcaatcgcgg ctggagcaat cgtttcatga agaatattgt tgtggtggat gttttccacg
     2401 accggaaaag tcgtgaaatc tccttgacca tttcctgcca ccatgatcga aatctctttt
     2461 tcaaacggga tccatgcttc taaaacacag gttccttctc ttaaaaggtc catggcagga
     2521 gctagatccg cccgactttt caaaacatac tgccccttgc catcatagcc gcctcgagtg
     2581 gttttcaaaa cacaaggata gccgatcccg tcaatcgcat cttggatatc cgtcggactg
     2641 acgatcgttg cataaggagc aatcacgatg ttattcgttt ccaaaaaaga tttttctaac
     2701 aggcggtctt gcgtaatcgc aagcaagtct gtcccttgag gaataaatga catgggtaaa
     2761 atcgcattta acgcctctac actgacgttt tcgaattcat aggtaatgac atctgttctt
     2821 ctggcaagct ctttcagcgc aaaggtgtca tcatagacac tttcaatgtg ccaatctgca
     2881 acttgtgcgg ctggacaatc gctcacagga tccagcacac ctactttaaa gcccatttct
     2941 tttgcactaa tcgccatcat acgacctaat tgaccgccac cgacgatccc gatcgttgaa
     3001 ccaggcaaca aaggtttaac caagttgatc actactttct ataacagttt tagccaacat
     3061 tgaacgtctt tcagccaatt tagcctccaa ttctaaatca tacatggaaa gcatctgtat
     3121 cgcaagtagt ccagcattaa ttgctcccgc ttttccgatt gctgtagtag ctacaggtac
     3181 tcctccaggc atttggacaa tcgaaagaag cgaatccaaa ccatttaatg ttcgtgattg
     3241 gaccggtaca ccgataaccg gcaaagttgt ttttgatgcg accatacccg gcagatgcgc
     3301 ggctccccct gctcctgcaa taatgatttt gattcctttt tttcgagcgt tttgtgcata
     3361 ttcaaacatc aaatctgggg ttcgatgagc agaaacgacc tttttttcat aagacacacc
     3421 gaattcatcc aatatttgac atgcttcttt cattgtttcc caatctgaag tactccccat
     3481 aatcacagag atttttgctg tcattgtttt tcctcccagt ttttctacat tcatatttta
     3541 acaatcactt tttttgatgt caaagaaatt tcgaatgtta acaaagaaaa acattttatt
     3601 gttcgtgttt attaaagatg aaaaaaagga atgtgacaaa agttaggtca cattcctttt
     3661 tatggataaa cggtgttcaa tcagctgacc ctcggtatta gctcaaacaa acggcaaaat
     3721 acgaggaact atttttccgt ttgtttctct aacaatcagg tcaaaacgct gatttcacaa
     3781 cctttctttt gctctattac ttcttgaagg cttcttccaa tatttcttca tcttttttca
     3841 atccattaaa gatcaaattc aatatgatag ccgtaatgct actcattacg atgccatttc
     3901 cagtgaacat ttgcaatgtt tgcggcattt gactgaataa tgtcggcgta acattgaaac
     3961 ccagcccaaa tccaacagaa acagcaacaa tcaatagatt tttatcattg ctgaaatcaa
     4021 cttccaacag catacggatt ccttggactg ccaccatccc gaacatgact aacattcctc
     4081 caccaagtac cggctcaggg atgatttgtg ctaacgcacc gattttaggg aataatccta
     4141 aaacaatcaa gaagaatgca gagaaataga tgggttttct tgttttgatt cccgaaagtt
     4201 gaaccaatcc gacattttga gaaaagccag tataagggaa tgtattgaaa ataccgccta
     4261 agataacggc aagtccttct gcacgatatc cctttttcaa ttcttcttct ccaacatttt
     4321 tcccagtaat gtcaccaagt gcaaaatata cacctgttga ttctaccatg ctgaccatgg
     4381 aaatgatgat cattagtaaa atcgaccata cgtcgaaagt tggtttacca aaataaaagg
     4441 gttgtggaac atggaacaac ggcgcatctc caatcgcgct cagatcaatc attcccatga
     4501 aagcagctaa gatgcttcct ccgactaaac caatcaatac cgcgattgaa cgaatgaacc
     4561 cttttgccag catttgcacg cctacaatta ggagaatcgt cacaaatgca agtagcagat
     4621 ttgttttgtc accaaaatca gctgctgttg cattacctcc acccattttc tcgattgcta
     4681 caggaatcaa agtcaaacca ataacggtga taaccgttcc tgttactata ggagggaata
     4741 gttttttaat tttagaaaag acaccagaaa ctaacacaac aaagatccca gaagcgataa
     4801 tagatccgta gatagctcct actccttcat tggttccgat caagatcagt ggagctactg
     4861 cttggatcgc acatcctaaa acgacaggca agccaattcc gaaaaatcgg ttaactgtca
     4921 attgcaataa tgtcgctaat ccacacataa agatatcaat tgaaatcaaa taagtcatct
     4981 gttcttgatt aaagcctagt cctgtaccga tcaaaagagg caccgctact gctcctgcat
     5041 acatcgctag cagatgctgc agccctaaaa ccgcagcttt tccattttgt gtttcttttt
     5101 ccacgattat gcgtcctcct caagaaactc aacttgtcca ttcgtcaagg aagcaatacg
     5161 cgcaagagaa acgactctca atcccatttc ttcaagtaac tgacgtccat cttggaaaga
     5221 cttttcgatg acgatcccga ttccttctac ttgcgctcca gcttgctggc ataattcgat
     5281 caatcctttt gccgcttgtc cgtttgccaa gaaatcgtcg ataatcaata ctttgtcttc
     5341 tgaagataaa aatttacgag aaatagaaat cgtacttgtc acttgtttcg taaaagaata
     5401 aacagaagct gtcaataatt cttcatccat cgtcaaactt tttgcttttc tagcgaagat
     5461 cattggaaca tctaattctc ttgcagcaaa taaagctggt gcgatcccag aagcttcaat
     5521 ggtcacaact ttcgtgattc cttgttcttt aaatacttct gcaaaccgtt ttcccatttg
     5581 ttccatcaat acagggtcta cttggtgagt gacgaagctg tcgactttta atacgccctc
     5641 gcctaatata cgtccgtctt tttgaatacg ttcaactaat tctttcactt tatttgctcc
     5701 tttatcattt attaataccg aaataaccgc ttttgtgcaa aaacctacta tgtttctgtt
     5761 aaagaaaaac accctccact ttatcataag aaaagaaggg tgaaatagcc aatcttttac
     5821 ttatagtcca gcatttacgg tactggctag aaactgtcat ccatattatg acgatatata
     5881 agtaggtatg taaaactaat aatagtgtaa attcattttc ttgcaactgt tttgcacgtg
     5941 aaggcagttt gccatatttg aataaaaaat gttgtttcaa atattgaaaa cagtctaaca
     6001 ctactttctg aaactgacaa cgtggtttac ttattttttt gtaaaatcta atgattttta
     6061 atgcattatt cctttttgtt cgggtttaaa tagttatttt tattttctcc tatctaaaaa
     6121 agcagcccac ataaaaagaa ggcatgacaa aaagcatgtc atagcccctt tttccgaata
     6181 aatagtgttc aaaagacagc ctatcggcaa taaggcaaga ttttataaac tacgggaagt
     6241 aatttccgta aatctattct tattctttta agctgacaag ctcgtcaatc actactgctt
     6301 ttcctgaagc aatagaatga tttgctgcga ttcctgtcaa aatcgacaaa ataccatcta
     6361 cgtgagttgc tgcccgattc cacggatctt cttctttttc ttcaccaaat agatcatcta
     6421 ataaacgcgg atctcctccg ccatgtccgc ccgcttcttg ttcgacgatt acctcatatg
     6481 gctcaccgaa catcggatag acatggatgg actgaagaac ggctgctcct tcatcctttt
     6541 tatctccacc tgcattgatg taagactttt cgatcacttt catttcgatc cgtcctttgc
     6601 tgccattgaa agaaacattg aatccttccc acggcatata cgcgtttagc gaatacgtca
     6661 aaatcgcacc agtttcatat ttgaccgtta cgcctagtgt atcttcaata gagatatcat
     6721 cggaaaatac actgcggtca cgaatatatc cactgtcttt ttctgcttct aaatacattt
     6781 ctcgcaattc ctcattggct gacaggtcta tagcaaatgg atcagcttta gctagttcgc
     6841 ttccatagca tcgactgtaa aatatttcta ctccgcgttt ctccgcatta tgcatcccat
     6901 aaaagtttaa tccaccgaat gcaaatacct tttggggacg gctgttcaac caaaaattca
     6961 ctagatcaaa atgatgggta gatttatgaa caagaagccc gccgctattg tgcttatttc
     7021 gatgccatct tctgaaataa tctgccccgt gttgcgtatt taacagccat tcgaagtgga
     7081 tagaaaacac atcaccaatc acatcacttt ctatcagttc ctttatcttc gtgtgatacg
     7141 gtgcataacg ataattaaaa gtgactcgca ggcttcttcc cgtctgtttt tggaccgtta
     7201 ttatttcttg agctttttcc tcatcgatag tcagaggttt ttcgcaaata acatcatatc
     7261 cttttttcat tgcacgaatg atataatcat gatgcgttcg atctactgac gtaataatga
     7321 cttcagccgg ccgtacgtca tccagtagtt gctcaaattg tgatggcata catgtcttta
     7381 cttttttctg tccatacttg ttcacaagta tccgatttgc ataatccatc cttgtttgag
     7441 atacatcaca aaaaccgact agttcacaag tttgatgata cgtagaggca attgcctcat
     7501 aaaaaaaacg cgctctccca ccaagtccga cttgtacata ttttttcttc atttaaagtc
     7561 attctccttt tcttttaagc aaaatactgt gacaaatggc agccctggat acagtaatgc
     7621 agtgagtaaa aaatcaggct gtatacacca gaacagtcca tacaaaaggc ataaatacca
     7681 cagaaacccc ttgacgtttt ttgacaacag ccagatattc cttttgatca aataaacaag
     7741 actatctgct ttatcttctt tgcagatatc aattagcgta ctaaaaaaac tatgggatac
     7801 aaaaagcaac agacacaata gaaaaggaat cagataccaa gctccaatct ttcccactaa
     7861 taaataccaa tcaactgcaa aaataatgac aaacagctga aacaacgtaa tgatgaaaca
     7921 catccgtttg attttccctt tcctccaaca tacctttttt tctgccaagt aatggacaga
     7981 tagataataa gaaggataca tcatcaaaaa ataaatcaga tagacaacac gagcagacag
     8041 cactaatcct aaagtgaaca aacatcctag aactaaagcg aaaagagcat tactgataag
     8101 actggttaaa aaaatccgac agacatagta gttgagcttg taaagaaaca tctcttctat
     8161 tggctttttc atattcagcc cttcagaccg cttgtactga tcccctggac aagatattta
     8221 ttgaagaata agaaaatcag aaaaaccggt agcagcgaca acgtagacat cgcaaacata
     8281 ctcgcgtaag tcgtactaga tccagcgtct gcaaataatt tgatggcaat cgataccgtg
     8341 taagatctcg gcttgtttaa ataaatcaat ggtcccatga agtcgtccca tttccaataa
     8401 aactggatga tgatcgtcgt gatgatcgaa ggtttcagca aaggaaaaat aattttggta
     8461 aagacactat acttgctgca tccgtcaata atcgctgatt catctaactc tttggggatg
     8521 ctgaccatga attgcatgat ctggtaaatg aaaaatgctt gtccaaaaaa atgtggtaaa
     8581 atcagcggca catacgtccc aaccaaattc agccgattgt aaataataaa ctgcggaatc
     8641 aaaatcactt gtgcaggaat catcatcgtt actaacatga cgacgaacca gaatcttttt
     8701 cctttgaaat cgattctcga taatgcgtaa gcaatacaag tcgagctcag aactgtccct
     8761 atcgtcgaaa ctgcagagat gacaaacgaa ttcctaaaga aagtaaaaaa tgaaattcca
     8821 gcaaaccccg cccatccttt tgcgtaattt tcccattgaa aagtctctgg aatcaaattc
     8881 agcgaaccac tcataatctc ttgatccgtt ttaaaagaac ctgcgatcat ccataagacc
     8941 ggataaatca tgactagacc taatagcaaa ataaaaccgt attgaaacca tttcgttact
     9001 gtattattca tttcttcctc ctctcattca cacaacgacc catttttttg atgatttgaa
     9061 aatcagaaag gtgacagctc ccataatcaa cagcatgatc cacgaaatag cactagcata
     9121 ccccatagaa ttaaaagaaa aagcttggtt atacacataa agagcataaa agttcgtggc
     9181 atcattcgga ccaccttggg taatgataaa tgcctgcgta aaagtcataa acgccgcgat
     9241 tgtctgcata accaagttat acaaaatgat cggcgaaaga agaggtaacg tgatcttgta
     9301 aaacgtctgc catttgttcg ctccatcgat ctctgccgct tcataatacg agcctgggat
     9361 ctctttgata cctgcagcaa agatgatcat tgccgaaccg aattgccaag cagacatcaa
     9421 aacaagaggg atcatgacca gtttttgatc gctaaaccag gaaagcgcag gtgcgcctaa
     9481 tcgttccagg aaaatattca ctaacccgtt tttagaaaac aactcccgcc aaactagagc
     9541 gatagaaata ctgccaccga tcaaactagg cacataatac aaagaacgaa agaaagtcgt
     9601 tcctttgata ttcctcgtta aaagataagc aatcaataga ccaaagatca gtttcacagg
     9661 tacagatgca aatacataga gaaaagtgac tcggatggag ttcaaaaaac gggcatcact
     9721 cagcatcttt atataattat ccaagccgat ccatgagact ggatttccca aacgataatc
     9781 ggtgaacgaa taataaagtg acaagatcat cggtatcact gttatcaata gaaatcctag
     9841 gatgaatgga gaagcaaata cgtaaccgac cacattgtct tgatccaaga accgttttag
     9901 tttttctttt ttcatgcctt ccttctgctg ttacttcttc ataaatgact ttttcaacta
     9961 aaagattgta ttggtcttga atttccggat tcaaagcact atcaaagaca ttcacttcgc
    10021 cgttgtccat atcggaaatc atttctaaat aatcaaatgt cgcttgcaaa gttgcatcat
    10081 ctgcacgagc gagctgttct cgaatattcg acatgattgg tacaccacgt tctcctttta
    10141 aaatttcatt ggcttcttca ctgttttgaa agaaatcaat aaacttagca gcttcttctt
    10201 tatgggctga gctttttgcc atactcagca tttgcgaaga acgtaaaggg ataccagatt
    10261 ctccatctgc ttttctgcga ggtggattaa tcaactgaat ctccatatct tcaggagccc
    10321 ctgacaatat ttctgtcaac tggttacttg atagaaacac cattgccgct tctctatccc
    10381 taaccgcctg aaaagacaga tccgtcgtac ttgcagctcc accacctggt tctggatggg
    10441 cccctttgtc taccagattt ttacgcagct ggaaaaaatc tgttagatag tctggatttt
    10501 caaatcccaa gctgttagat agatcttccc gattataaaa attcaatcct ttctcaactt
    10561 gtggtatttg aataatacag ccagctataa aatcttcgaa tcttgaaata gcatagattc
    10621 ctaattcttc agtgatttga tagctgattt gttcaaattc atcccatgtc caatttgaag
    10681 ctggttcagg gatatttaat tgttcaaata tatttttatt gtaagcaatc gcaaacgaat
    10741 tcaccccgtt agacagtcca actaattgcc cgtcgtaagt cgtcgttgct aaataggatt
    10801 catctgcatt agaaatatca attatccctt gctccacata ttcatctaat ggttcaatct
    10861 gattgatgta ttgaggaaat tgattgttca gttggaaaat gtcccagata tcatctgatg
    10921 ctgctagtgt acctaatttt gtgtaatatc cttcataatc ataaaattca taatcgatcg
    10981 tcacatgggg atttttttct tcataaagct cgattacttt tactgttgca tcgtgtcgga
    11041 cttgcgaacc ccaccaagcc atgttcagcg tgattttttt gtcatcttct tgcccacaag
    11101 ccgtcaagaa gaataacata acacttaaag ctaagatgat tctcattctt ttcttcattc
    11161 tctgcacctc tacttccatt gttatttttc aatatactgt atttttatac agaacatatt
    11221 tattaaacga tctttttcaa ggggaataaa aaccaagact tgccttatcg ttctgcctag
    11281 gatgaacaac tcaatacttt caagaaaaat tagatagtgc tgattcttct ttcatcgcta
    11341 aaaacacttc ttgcttcaat gatgggagga taattacgca aagaaaaaag gatatcagac
    11401 aactgcccaa tatccttgac atagctaaat aagcatgctt cttcgtgtgc gctcttccct
    11461 aaaaaacaga aaaataacta gtaattctgt atgaaattag gtacaatata cacgtcgatg
    11521 cacaagcatt gactatggca acttgattgg tcagttcccg ctgacttttc aagccccgac
    11581 aatttagctg ctactaaatt gtcgggtgcc ttttttagtt tttattcgtt tttcattcta
    11641 tagaaagcgc ttcaataaat caagacattt ttttgttaga atgctaataa taacaaaagg
    11701 ttttaactct tcttatgtac attacaggta aaaaaacaaa aaaatttctc ttttttttga
    11761 attctgaaca aatagtactg gagtacaaaa gacgaggaag agccactatc tgctcttcct
    11821 cgtcttttga ttctcacctg ttattcatga ttctttttct cagtttcccg atatttttcc
    11881 attttacgac taaaaagctc tccgctactt ggtggcgtat atgtgatggc atttgctcct
    11941 gcttgaatgg tctcagcaat actttcttcc gttggaccac ctgtcgcaat gataggaagt
    12001 tcaggatatt ttttacgaaa atggcgtact gcttctgcag tttctttacc tgcactgata
    12061 ttgataatcg aaataccagc tgctaacttt tcatctattt ctgtatattt cgacgttacc
    12121 gtactgataa ctggaatatc aactactcga caaacttctt ttactgtctc taaaggcgta
    12181 ggaccgttca aaacgacccc taacgaacct tgcgcttctg cgaacatgct catgtaggaa
    12241 gaacgagcac cttgtgtcaa accaccacct acaccggaga aaactggaat gtcagctgcc
    12301 tcaataatgc ttttagtaat cgctggatga ggtgtaaaag gatacacagc aattactgca
    12361 ttcgcatttg tattacgtat gatcgcaata tccgtagtaa agatgatcga acggatcaat
    12421 tttccaaata tcttgatccc gcttgcttct aaaatgattt ccggtactct tacgatatct
    12481 tttctcaatt ccgtcatgat ttctggcgta aattgttctg ccatcagcca cttctccttt
    12541 gctatctccg gtcatactga ttcaattgat acgtctcgat gacctggatg attttttgcg
    12601 cataaccggg gtctgtcgca taacctgcat cttgcaatgc ttgtgcggct tcctgatagt
    12661 tggtggccgt aatcactttt tcatacagcg caggattcca atctactcct tggacgaaca
    12721 gttgtgtatg gtcatccatg gactgttccc atgaatcata cactttgaag tctccttgga
    12781 tagttatcca ttcttcgtta acaaattcct ttgtttccag gcttactttc ttctgatccc
    12841 cataagcctt gatcccgaaa agattattgt atttgctagc caaggtactt tttccccaat
    12901 ttgattctaa tatagcttgt cctaaaatga tactaggaag cactccataa ccatcttgta
    12961 gttcttgggc atgcggaaca agccgttcaa taaattgctg atgggattgc gcttcatttt
    13021 cttgctgatt atcagtaaaa ggagaagaca gattcttcaa ggaaaaaaca aaggctaaga
    13081 gtatcactgc tacaccggca accactgctg gccaacggat attcttgcgc ttttgctttt
    13141 tccacttttt tttcgccatg atcaattcct acttttcatt taattcattc agatattctt
    13201 ctagcgtcaa ttggtgtttg gtgatatact cagcgttttc tttaccgaca taacgtaagt
    13261 gccaaggttc atatgtgatc ttagttatat cttcgtgtcc ttttggataa cgaacgataa
    13321 atccatactg cgcagcatta tctgcgatcc atttagctcc aggctgatct ccgtaacttg
    13381 catccaatac agtagaagga taactgttgt accagttttg atcgacgaca tcgactgcca
    13441 gacctgtgtg gtgttcgctg tatcctggct ccgtaatcgt ttctttagta atctttgtcg
    13501 cctcctcttc tgatacacct tgacgactca tcacatcttg gacattttgt tcgaaaactt
    13561 gttgttggga tgatacagac cggtaagctg agaccatcac taatggaaat ccagctgctt
    13621 cagcagcctt ggcaaatgct tcatattctg gagcaatgcg tttgtcgatc aagtatccgt
    13681 tacttaatga tgccaattga gtagactcat caatttcttt ttctaatttg tgatctggac
    13741 caaccaaaac tagcttccaa tcgtctgttt taacatctgg caattgtttg gacgtacgat
    13801 cttttgtttc tttgtccttt accttgctgc tggttgtttg actgttcttt tctttactag
    13861 cagttgtttt ggtgcttgtt cgttcgactg cttgattgcc ggcttgaaca tgcgaatcat
    13921 catcgctaaa aataacatag cccattgctg cgatcataat cacaactgct ccaaatccta
    13981 aaaatttatt cacttgaaaa atcctttcgt tgttcttcac gttccccatt cgcctcatga
    14041 acgtaagttt cttccagatt ttcattcacc caatcgagaa gttcgatttt ttctccttcg
    14101 atctgttttt gaacatctgc tggcagacga aaattcaaaa tatcatcata gccccattca
    14161 tcatattgaa tacttacttt caagtcaagg tatctaccag cctgctgatt atttttcaaa
    14221 gggatcagct cgacttttgc tgctttgctg ctaagccatt tttgcgcgat gcgtgttttg
    14281 atttgcttgt actctgcaac ggcaacgttt cgtttttcgg gaaaaataat cgtttgacgc
    14341 aaaatctttt gcgttagcat ccattcataa tcagtgaaaa gattttttac tttctgcatc
    14401 gcttcagatg gcagattttt ctcgccatct ttccattttt gccattgttc tggactgatc
    14461 agcaagtatt catctaaaaa atttttttca gtttcaaact gttctaataa tacactcata
    14521 ataagatcaa tatatacttg atgcacgttt atcccctcct tttaactatt ctacttaagt
    14581 tttgatcaaa aagcacctct taaatcgctg ctttcatttt ttttaatgtt tcaagtcctc
    14641 tcctctttat tcttcgtgat ttttgttata ataatagaca gacaagaaac tcaatccaac
    14701 tatgaaggag cgttttcaga tgaaaaaata tcaatgggga atcgttggtc ttggtaccat
    14761 cgcacatgag tttgccgaaa gcttcaatca agagacgagc gaactaacag cagttgcttc
    14821 cagaacttca gaaaaagcgg aaaacttcgc tcaccgttac aatattccaa aagcttacgg
    14881 aagctatcaa gaaatgttag acgatgctga aatcgacatc gtgtacattg ctgtaccaaa
    14941 tagacagcat atcgaccata tcttggcagc attggaagca ggcaaacacg ttctatgtga
    15001 aaaagccatc acgatgaata aaaaagaact ggctgacgct atgcgcttag cagaagaaaa
    15061 gaatttgatc ttagcagaag cgatgacaat cttcaacatg ccactttatc agcagttacg
    15121 ctcaataatg gatacaggaa aactgggggc tttaaaaatg atccaagcac ctttcggcag
    15181 ttacaaagaa ccagacccta aaaaccggtt cttcaatcca gaattagctg gaggagctct
    15241 gttggatatc ggtacgtatg ctgtatcttt cgctcgtttc tttttgagct ctcaacctga
    15301 agtagttgcc tctacaatgg ttccttttga aacaggcgtg gacgaacaat ccgtcactat
    15361 cttgcgtaac aaagaaaatg aacttgctgc tgtcagcttg acttttcagg caaaaatgcc
    15421 aaaagtcggt atcgttgcct ttgagaacgg ttatgtgacg attgctgatt acccgcgtgc
    15481 tgatcgtgcg gagatcttat ttacagatgg tacaaaggaa ttcatcgaaa gcgggaatac
    15541 atctgaagca atgaattacg aaataaagaa tatggtacac atgattgaag gtgacttgcc
    15601 caatcgctct ctcttcttga caaaagatgt catcgaaatc ttggatcaaa tgcaagttct
    15661 atggaaaaat atgtaatttt aaaaggttgt gaaaaagctg ttctgcatca agtaataaga
    15721 acagccaaac aaaaatagct cctcctattt ttcgtttggc tggacttatt accgcagagg
    15781 aaagcttttg aacaccgttt aagaaagagg ttgtgagcct accgttcagc tccgagtcac
    15841 aaggagcaat ttcaaaaaac agcttctcat gtttgttgaa tattgtgact tgtgaccgag
    15901 gagttgacag gagaacaccg tttaaagaag aggttgtgaa atcagctttt tgacctgaat
    15961 attagctgaa ttcacaacct ctatttttgg attttttgtt gggagaattc tgccttgatt
    16021 tcaataaaac ttttaaaaga ctgcaaaaac tggaacaatt ctgctcgatc ctcgaattca
    16081 gggcgactag tcggcagagg catttgccga tacaatacat aaacttcttc aatacgtgtt
    16141 aaaatttgtc tgccatcatt tgactctgaa aatgtttctg cagtataaat caataaacca
    16201 tagatatgtt ttccataata agctggttgc tggattctct ctagattttc cagcatgtct
    16261 ttaatcacat tggcctgtgc tcttctcata gagaagtaag tttcgtagta tagcggctgt
    16321 ctgagccagt agttttcttg atgttctctg gcatcttctt ggctttcgcg aatgaatgtc
    16381 agtaaatgtt cgcatttgat ctgcacatct tctttatttt ctgataaaag aaattctgcc
    16441 atttgtctca aaatatttcg aaattctttt tcgatctgta tttggttttc cttcagctgc
    16501 ttcgtacgat ctggcatgta aacatttgca agcaaagcaa atactaatcc aatcgccatt
    16561 aatcccgctt cattgccgat cagctgccaa gacatgtttt cctccactaa ataatgagtg
    16621 accaaaaccg aatttactac tatcccttcg gttaatttga agcgagaggc aagcgggatg
    16681 aacaggaata aaaaaagact gaagccaaaa atagtatacc caacagcaaa aaaactgaac
    16741 agtgctgttg ccgtagctaa tataaaggct gcgattcgat tcatgcccgt acgcaatgta
    16801 gagcgcttgg tatttcctac acttaataat gcaatgatcc ctgcagctgg ccaatacaac
    16861 agcgataagc tacccgctgt gaccatcgaa aagaagacac tgatcactgt tttgactgtt
    16921 cgcattccta tacgcattta cttccccctt ttttatttta attatacttg aataatgaaa
    16981 aactagctat cagacaaaag tttaggtatg atagtaaaaa gaccaaaaag gagaatctct
    17041 atgcaatttg gccgttttcg actaggtatg cgtacgatca aaagtgcctt agctgttttt
    17101 ttatgtatct tgttttttca tttgactcaa cgaggaatcc cgatgattgc tgcattatct
    17161 gctgtttttt ctttgcgtca ggacttgact acaaccgttt cttttggaaa gtctaggatc
    17221 atcggcaata cattaggtgg tacattagct atcatttatt tttttgtcaa aagctatttt
    17281 cacaatgatt ttttggtaga attgatcttg cttcctattc tagttattat tgtgatcgtc
    17341 gtgtcagatg gtattaacaa taattccggt atcatttccg caatagctac gttgttgctg
    17401 attgcgttga gcatccctca aggcgaatcc gctttgtatg ctatccaacg tgtactagat
    17461 acttttatag ggacattcat tgcaatcggt atcaatttct ttcttcgtcc tcctgaaaca
    17521 gaaaagaagg aagaactatc agaagatttg gtcgaactcc aaaagaaaga acgattgcta
    17581 agaacaaatt tacaagagat agaagctaag atccaaaaac aaaagaaaaa tagcgaggac
    17641 taattttctt ttgcaataaa ataaaaaaat ggagagaaga actttaaaaa gtcttcctcc
    17701 atttttgatt gaacggtgaa gtgtactact ttattcttcc ttcatcgagt tttgttctgc
    17761 ctcttttggc gcttcataca tatttacttg gctagtagtc ccacgtgtcg acaaattctc
    17821 tacgatttcc ttcaagtcaa gtcctgttgt cgcttttagt gtttcttgag tagaagaaag
    17881 caaatttgtg gcataatttg tcactcggtt cgctccactg gtttctcctg aactgcttcc
    17941 tgtatcgaca acagagattt tttcgatatt tcctagcggc tgtgctgctt cgcgcatcaa
    18001 ttgcggtagc atatcgataa ccatactcaa gacagctgct tcgccatatt ccttgaaggc
    18061 attggcgatc ttttgttttg cttcggcttc tgcttttcct tttgctagtg ctgcttcggc
    18121 ttctgcttgt ccagctaaac gtgtcttatt ggcttccgct tctgccagtg cttccacacg
    18181 gaatcgttct gcttcggctt cagtcacttc tttgactttt tgagcttgtg cttcttgttc
    18241 tttagcgtac cgatcggcat ctgctttttt cttcacttcc gaatcatatt gcttttcacg
    18301 acgtgtgatt tctttttctt ccagttcgat ttgtttttgg cgttctacga ttttaatctg
    18361 catttcctgt tcgattactt gttgttgtgc tcgggcactt tctaaattgt aggcttggtc
    18421 tgcttttgct ttagcaacat cttgttcttg tttgtaggtt gctaatttca gttctttttc
    18481 ttttaatgat tctgcgatct ctgtttgtcg ctgtaattca gctgcttgtg attctttttc
    18541 tgcttgagct tttttgatac gcgtttcttt tagagcttcc gcttctgcta tatctgcatc
    18601 tcttttgact tgggcgattc ttggtttccc taaagagtca agataaccgt ttttgtctct
    18661 gacctctttg atagtgaaag aaacgatgat caaccccatt tttgcaaggt ctacagaagc
    18721 gacttcttgt acgctttgac tgaacttatc gcgattttga tagatttctt ctacagtcat
    18781 agaaccaaga atcgagcgca agtgtccttc taatacttct cttgcttcat tttccagttc
    18841 ttctcttgtc ttgcctaaaa actgttctgc tgctgtagca atctcttcta cagatgaacc
    18901 gattttgatg atcgaagtac catcacacat caccggtacg ccttgttcgg tatacacttc
    18961 aggggtggag acgtccaatt tactcgataa tagactgatc cgattcgaac gttgaaaaac
    19021 tggcaagacg aatgcgccac ctccacgcac aattttgatt ttgttattgc tttcatcaat
    19081 gtgcacattt ttgcttccta aataacttcc actgatgatc aacgcttcat ccggttttgc
    19141 tgtctggtac ttcgctacaa agacgatcag caacatcagt aaaatcaaag ctgcgattgc
    19201 gatcgcgata aaaacaactg gtatttccat ttaacacacg ctcctttaag cttttatcat
    19261 gctttcataa ggcatgacat aacagattct ttccttgatc tcaatgatca atactttcgt
    19321 tccccgctca gctgtctgtt cttgggggct gtaaaaacaa gctggacgac taatggaacc
    19381 ggtaacactt ttcaactgaa tttctcccat acctcgtaca gggatcggtg tgatcacaac
    19441 agctacttgt ccttccagag ttttttcaga agacgacatc gttgattcgg catgtttcat
    19501 cggcatcaat atatagaaat taagcaaaaa taccaacact atcgatatgc ctccactagc
    19561 aataaataaa aataaagatg attgatctgt taaacgctca cctagaaaac caaataagct
    19621 tgtgaaagtt aaccaaggga taataagcat cggatcaagc gggccatcaa aatcgaatac
    19681 atctccaaaa ataatcaata ggactgccaa tcctgcacaa atcagcaatg tataccaata
    19741 aatcgtctca agtgcgattc cccctatcat atacacgcct ccttccattt ctacgtctat
    19801 cataccaatg tttgaaaatt ctgtatagct tcttttaccg actcagtggt gttttttata
    19861 ggaaggttgt gtttatcgtt gacaatttgt taaatttttg ttttcatgtt aaaattttca
    19921 caataaattc tttcgaggta gcctatgttt ccttttttta agaaaaaacc attaaagctc
    19981 cagaacacga tcatccttgt cgtttgtttc tccacattcg tttctttgat tgtttctgcc
    20041 atattgatca ggaattttat tctttcgatg aaccgatcta gtgtgtcaga taaaatctct
    20101 agcattgcta aaatcgttgc caatgataaa ctcatccaaa aaaagctgca gactggtgac
    20161 ccgacacatg cgattcaagg aataacaaat gaaatcatga ttgcatcgaa tttagatttt
    20221 atcgttgtca tggaccctga aggcattcga tattctcacg cagacccaag ttacatcggt
    20281 cttcctttct caagtcatga agatgtgaaa aaagtgattg gaggacaaga atttttctct
    20341 gaaaaagaag gaatgttagg gttcggcttc cgctatttcg tccctattta ttatgatcaa
    20401 gagattattg gtgcagtttg tgttggctta acaaacaaca cgattgacaa tcaagtcaaa
    20461 gatggacaaa aaatgattgt tttttcattg attattggac ttagcgtagg catcataggc
    20521 gccgtttttc ttgcccaaaa aatgaaaaaa gtactgcttg gacttgaacc acaagaaata
    20581 gcagaacagt taagtgaaaa aacgattatc gaaaatgaag tagatgaagg aatcttagcc
    20641 atctcttctg ataaaactat cctcttgatc aatacacctg caaaaaacat tttctaccaa
    20701 acagagcagc tattggattt cgaagtagga aaaaaatcag tgataatcta tatgacgttt
    20761 tgtttaaaga gacgattgag catcaaacga ccgttcggac tcaatccatg tatttaaatg
    20821 ggatagaaac tatttctagc gttacacctc tttttagcaa tgaccgtttt ttaggagcag
    20881 tcgcaacttt acgaaatcga tcagaagtaa tgaaattaat ggaggagctt ggggggacga
    20941 aacaatatat aaactccatg agagctcaaa cccatgaatt catgaataaa cttcatgtca
    21001 tttctggttt gattgatttg aagaagtatg aagaagtcac ccattttatc cagcagctaa
    21061 atcaaaatta taaaaaagag attggccaag tctcaagtct gatcaaagta cctgcaattg
    21121 ccggtttttt attaggcaaa ataaacgaag ccaaagaaca atctgttgaa atgctgttag
    21181 aaagcgattc ttttatccct aaaatggaga tggatgactc catccattca cttttgcaag
    21241 tgttagggaa cttacttgat aatgcaaaag aagccgtgct acaaaaacat gaaagaaacg
    21301 gacaggtccg gattcattta aactatgaca cagaagctga agtattcatc gtgaccgtct
    21361 gcgatggcgg tgtcgggata tcagaagaaa tctcagaaaa aatatttacg ctaggttttt
    21421 cgacgaaggg aatcaatcgt gggtttggat tgaatctcat ccattcaatc gtcaaacgcc
    21481 acggtggatt tttagaattt tccaaacaaa caaatggcgg tacaatcgtc tatctagaat
    21541 taccaaaaaa ggaagatcaa aatgatgaca aatgtcctga taatcgaaga tgatccaatg
    21601 gttgcagaat tgaatcgtac ttttgtggaa agcatcccta atatgaacgt aatcgaaagt
    21661 ttcagaaatg tagaagaagg aaaaaaatat ttcagacaaa aaaacgcaga tctgctgctg
    21721 cttgatgtat acatgcctgg cgaaagtggt atccattttt tatcttggct tagacagcaa
    21781 ggaaatgata tcccagtgat tttgatcact gctgcagatg atatcgaaac cataaagaat
    21841 gtgatccgct acggtgcctt agattatttg atcaaaccgt tcactttgca acgttttcag
    21901 ctggcgatcg aaaagtatct tgctttctct cagacaatcc aaacgactga aaaagccgat
    21961 cagaaattga tcgatcaagt attcaaccac accactccca ttcacgaaat accatcattc
    22021 atcgatttgc ctaaaggtct gtcaaaactg actttaaaaa aaataagtgc atttattcta
    22081 aaagaagata ctattttttc aacaaaagaa ctggctcaaa aaagctcact gtcacgtata
    22141 tccgtcaaga aatatttgaa ttttttagta gaacaagata tattgaacga aagaatcaaa
    22201 tatttggaaa tcggccagcc gctaacctgt tatgaattga caaagaatgg gcggaaaaag
    22261 ctcacagatt actgttgagt catcgaatta tcccatacaa aatagtccaa gtatacagaa
    22321 gtaacaactg tttacttgga ctatttttaa acattttatt gacgaatatc catgttttct
    22381 tatcttaatt gacgggcttt aggaagagat acctcatttt tcgtttttcc agtttctatt
    22441 atttcacgaa agttctcgaa acttgtcgaa atcatatttg ataatgcctc atctgtattt
    22501 gaacctacat gcggggttac aagaactcta gggtacattt ctactaattg ctggatagca
    22561 gggtctggaa tcgtctccca tggcttgaag gatcggaaaa aaacctctgt ttcatttgca
    22621 aaaacatcgg ttgcaaaacc agctaaatga ttgcttttca aagctcttaa aatagcttca
    22681 ttatcttgaa gctctccacg ggctgtatta atcaagatgc tacctttttt catttgagct
    22741 aaaaatcctt cattgatcat ttgatcgttt tcacctggga tatgaggtac atgaatactg
    22801 acaatatcgc ttttggctaa taactcttca agcgagtgga acgtcaatac ttttttagca
    22861 gcttctgtct gatgaatatc gtagccgatc actttggctc ctagtccctt aaacaaaaca
    22921 gcttccgtca accctatcct gcctgttcct atgatcccta ctgtgcaatt tctgatttca
    22981 cgactaaaca tttgctcatc cactcgaaaa tctttgaaag atgttcggat cgtcgtatac
    23041 gcagtatttc tcaaaagcat catggcaaaa gtcaaagata actctgctat cgcatttggc
    23101 gaatacgatg gtacacgggc tactgtcata ccaaaatcag ctgccgcttg tagatcgata
    23161 tgattgactc ccacagtccg tgtaaaaaca taacgaatcc catattcctg catttttgca
    23221 agattggtct catctgctat acaatttccc ctcaacaata cagcatcatg gtcctttgct
    23281 gacacgatat tgtcatgtgt caacagttct tcataaagac tgagatcata atggtaaata
    23341 ttcaattttt caaaaaaatc tatctcgttc ggacgaacac cgtaacatgc gattctcata
    23401 ctttcttcct ccatttctta tgaaaataaa cctatgccat gaatcatttg taatacaacg
    23461 atccatacag gtgtcagtac cacagcaaaa atagttgaca gtaaggatgc attggaggct
    23521 aaaagtgcct ccctatcaaa actgatagca taggctgctg ccactgttgc agctggtgta
    23581 gccatcatga taacgattgt tgccagtgct tcaaaattga ctggtagaat attcgttgcc
    23641 gtcaaaactg ctagaatcac gatattcaat gcaggtacaa ataccacttt gactagactg
    23701 taataccatg aagttttttc agatgcagct tttttgaagc ttacttcacc taatgttgcg
    23761 ccgattgcta gccacgccaa tggtgaactt aaaccagcta aataatccat cggctgaaat
    23821 agccacggtg cagtctggtc aattcgcaaa aacgctactt ggcttatctc tccttctgca
    23881 cctgtaactg acacttgtgg cagataccct tggaacagcc aaatgaaaag gccggcaaaa
    23941 gtagcgataa tgattggatt gacgaacatc gctttgatat ttttagcttc catcttcaag
    24001 ccactcattt ttatgtatcc ataagaatac aaaaagattc ggtagccaat attgaaaatt
    24061 gaggaataca tcacgccgac aggaccataa atcgcactga cgataggcgt tccgaaaaag
    24121 gtcgtagaac caaagatcgt caggacacgc aacgttgttt gcttatcccc tttatagctt
    24181 aagaaaaaag gttttgaaac tagaataaga ataatataaa taactaatcc ccaaatcaag
    24241 acattgattc cttgcatcag taattctgta ttgatatctt gcatgaaagc tcgaaatgcc
    24301 agtgctggca aagccactgt taagacgact ttcgatagaa tttttccaac agattcagaa
    24361 aaaatcccct tttttctaca aacaaaccct aataatataa taaaaactgt tgaagtgatt
    24421 gcactgacaa tcgacatatc tgtcaaagtc gatcgaaaaa tgtcaaataa gttcatcttt
    24481 ctcctcctaa ttatgttcat ttattcacaa taaaaaaagc ttttattttg ctaatacgta
    24541 ttaacatttt taattttatc tttacattgt tacttattga acaataggaa attaaagaaa
    24601 cttttgaaac ttatgtaaag tgatttttac tagaacacaa aaaaacctta aaaccaaact
    24661 taagaagttt agttttaagg ttaacttaaa ttatattatg cgtggaaacg ttccacgcca
    24721 tctagataag ttgcttccaa tgtcatatca gggttcaata cgataaagtc tgcatcgcgt
    24781 ccttgagcga tttttccaca tgtatcatcg attttacagc tgattgctgg aatcagacta
    24841 gccatcatga ctgcttgttc tggtgttgct aatccccagt ctacaacatt tttaattgct
    24901 tctttcaatt tcaatatact tccagctaag ttgccagatt ctagacgtgc tgttccttct
    24961 tttactacta ctggaaattc gcctaaattg taatttccat caggcatccc accggccatc
    25021 atacagtctg taattaatgc gacatgatca tgtcctgctt tttccattag gattcccgca
    25081 gcatttggat gtacatgatg gccatcacaa atcaattcag aaaagacatg ttctaaagat
    25141 aataacgcac ccaccatacc tggttcgcga tgattcaatc cacgcatacc attgaaagca
    25201 tggacaaata cacttgcgcc tgcttcaaca gctttttgcg cttcttctag tgtagcatta
    25261 ctgtgtccta gagaaacaac tactccttct tcagtcactt gtttgacaaa ctcttctaca
    25321 ccttgacgtt ccggtgctaa agcaattttt ttgattaaac cacctgaagc ttcttgccat
    25381 tcatggaaag tatccaaatc aggatccccg aaataacttg ggttttgtgc ccctttgtgt
    25441 tcttctgtga agaaaggacc ttcaaaatag attcctttga ttttagctcc tggtgcttcc
    25501 tgatacattt tgccgattgt ctcagctaca tcttttaaac gttctttgct tgacgtcaat
    25561 gttgttggta aaaatgaagt cactccacaa gataacagac cttcagacat cactttgatg
    25621 ccttctgcat cattatccat tacatcatga ttcatatagc cgtgaatatg tgtatctacc
    25681 aaaccaggag caatccattt gccgctttgg tcaatgatgt tgacttctcc ctcaggtagt
    25741 tctttggtgt attcaccaaa aagtccatcg gtaatttcaa gatagcctgc atgtttgaca
    25801 cctgacgcta aaaagaattt gtcagcatag ataaaagttc tcattctatt ctctcccttt
    25861 gtttttctac ctctaaagta ctcttattta gagaagaagt caagaatggt ctacaccatt
    25921 tacaaatttt ttgaaatttg tttcacagct tgtgctaagc cttcatagaa tgctgctctt
    25981 tctggttctt ctttacgctt gtatattaag cttttaagca caaataaatc attgaggtaa
    26041 tagtagctat tcttctcttg agcccaatag attccttgat tcacctgttc taacgcttcg
    26101 tcgatttcat tttgatggaa taagaaattt ccataattat aaaaaatttg cgaaaattca
    26161 ctttttgatc gttcatcagc tgattgatgc agcagttgga tgcttcgact caaatagtag
    26221 cgtgcttcag ccgtttttcc tgccacacca tatatttttc cgatacaact aatcagttgt
    26281 atttctgtgt cagaaacatg gattctttct ttttggctag agtagcttaa tgcttctttc
    26341 aaataataaa gggactgaga gatattttga cgcaacgtga attcgcaaac accgagataa
    26401 taataatagc gttgaaagtc agttgcttga tacagatttt cagaaatatc agaagatttt
    26461 agtacttgtt ctaacttccg ataatcacgt ttttggaaac aactatccaa cagttcaaat
    26521 aatttatcgt ttgtccgagt agtttttcga gagagattca tcacatgatc aatcgttacc
    26581 cctagccgtt gacaaatctg catcatcacc aatacattcg gcagttcctc attattttcg
    26641 atccgactta atacactttg tgaacaaata tcatgcgaaa gcattttttg cgtcattttt
    26701 tgttcttttc gaatctcttt aatgatcgtt ccaaaggact cgattaacgt ttccataagt
    26761 atcctcccca aaatatgcgt attcgcattc tattcgtatt ttactttagg aagtcgcaaa
    26821 cttcaagaaa agacttgcaa ctaaataata tactttagtt atttaagaaa actccactag
    26881 aaagagagcc tcagtgtgtt tgtctgtggc cctcctactg aaaaatttgg tttttaaaat
    26941 catttttggc gataggttgc taggaattct ctcattttct tagctgtttg tttcaaatca
    27001 tccattttag gaagataaac gattcgaaaa tgatctggct ggttccaatt aaatccacca
    27061 ccatgtacaa gtagaatatg atgttcatgt aaaaagtcca aaacgaattt ttcatcatcc
    27121 aaaatattga atcgctttgt atcaattttt gggaatatat aaaaagcagc ttttggtttt
    27181 acggctgaaa gaccaggaat atcattgatt gcattataga tatattctct ttgttcatag
    27241 atccttccgc ctggcaggag cagctcatcg acactttgat aaccgcctag cgcggtttgg
    27301 ataatttgct gagacaatac attggaacac aaacgcatgg aggacaacat attcagccct
    27361 tctatatagc ctttgacgtg tgacttatct ccacttagga ccatccagcc tactcgaaat
    27421 cctgctacac gatgagattt ggatagaccg ttcagcgtaa caacgaacaa atcaggcgct
    27481 aatgtagcaa tcggaatatg ttctagacca tccatcacta agcgatcata gatttcgtcg
    27541 gaataaataa tcaggttatt ttgtcgagct acttcaacga tttcttctaa taattctttt
    27601 ggataaagtg ccccagtagg attattagga ttaatgataa caatagcttt tgtccgactc
    27661 gttatttttg atttgatatc atcaatatca ggataccatt ctgcctcttc atcgcaaata
    27721 taatgaacag gtgtgcctcc agctaatgca atagaagccg tccacaacgg atagtccggc
    27781 attggaacca gtacttcatc tccattgtcc agcaacccct gcatacacat cgtgatcaat
    27841 tcgctcactc cgttgccggt gtagatatca ttgatcgtca cattcggaaa atgtttcagt
    27901 tggcaatatt gctcaattgc ttttctagca gaaaagatcc ccttggaatc tgagtagcct
    27961 tctgagtcac gggcattcat gatcaaatca cgaatgactt cgtttggtgc ttcaaatcca
    28021 aaaggtgcgg gatttcccgt gttcaatttt aaaatactaa tgccctcttc ttgcatccgc
    28081 tccgcttctt ctagaacggg accgcgaaca tcgtaactga caccttctaa tttgttggat
    28141 ttttcaaatt ctctcatcac ttagccaatc cctcatttca aaaagttaga actttccaaa
    28201 ttttttgtta tttactattt caatatcata ctccatccta gaaaattttg ccacttattt
    28261 aggaaaaacg taagtattaa aaaaacacaa acaagcgttg agaacttaat tgtcatgata
    28321 gtcattccat agattcgctg cccaatccat tgcaattcca caaatgattc ctagcataaa
    28381 accaagcaaa aaacgtccga atgcaagtcc aatcaataca cctaagatca ttcccagtcc
    28441 tgaaaattct ccgtcatatt tctccatctt aataagtcct cccatttttg agcttgacaa
    28501 ctatgtaaaa cctcgctatc attagtgtgt tattattacg cagatgtgtt ggaattggca
    28561 gacaagcatg attgagggtc atgtgagcgt tagctcgtgt gggttcgagt cccatcatct
    28621 gcactacaac tcgtgaagtt aattttgctt cacgagtttt tttgtttgaa cgtttcttta
    28681 tgccattata tacatatcac tgtatccttt acaaaaagaa cgtacaatca tgtaaacagc
    28741 ctgaggtgag tatatgaagg tcggaaacaa gctactcgta gaaacttgca agtgttaaaa
    28801 caattaatcg aaaaacgagt aaaatccctc acctaatact aaagcgaggg attttttgta
    28861 tattatctgt ttatcaccgt tattataaag gtacaaatac caagcacggt taatccaaat
    28921 tgcataaatt catttttttg tacactcatg cttttcatta gctctttaac tgccattagt
    28981 actaagatgg cgataacaac ataaagtata tagtacgtcc gcattccgga agcccatact
    29041 gtgggagaaa atcccataat taaccttgaa caaaatccca ttatcatcaa aataactaaa
    29101 aaaatagaag ttttcttatt gtcaaaactt aaataaattc caacaattaa acatagtaat
    29161 aatgccaaga ctactaaagt agctatccaa gtacctggat aaaaaagtga cagtttcgtt
    29221 cctaactctg taaagagatt attcaggtta ctactactcc aaatcattgc ccttttattt
    29281 ccattgacgt aagtaaagct ttgacccatt gtattaccaa aataaataat caggtttaag
    29341 aaaaacggta atgcagtcaa tatacgcaca taatagtttt gacatttcct atatgttaga
    29401 aggaaaatta agaaaaatag caataaaaat aagatattca tgtctaaaaa aaacggcttt
    29461 ccaaatgatg aaatacctaa atctaactta ttaagaaagt tgaaattttt atattcagga
    29521 aaccatttat taatttcatg tgcagcacgt aatgcattcc ctggagttgt caaagagaaa
    29581 attaattctg ctaagcttat tattgaaaat actgataact tataattata tcgtccattg
    29641 acaatcaaat ataaactaac tatacttgtt agaacaaaaa aacaggcgtt gacttgttct
    29701 tgattagtta aaaatatcaa taaaggaata cttacccaat atatttttct atttatttct
    29761 tctcctctta agagttgaaa aaacggataa aaagttagta aactaaatgc tactggccac
    29821 tgatagttca aggtggttgc tctccaaccg gtttccccca ttactgtcaa tggtattaaa
    29881 cagtatatag agcaaaccaa taataaattt ttaacattta ttttctcaaa aacatacttg
    29941 caaagtaata ctacaattcc taacataatc gtagaattta ataatttcca tagcataaag
    30001 tgtttagaaa agaacattaa gaatagttct ataataattc ttgaactcca tgtcttataa
    30061 tgttctatcg aagcacccaa atagccaagt tcatctaatc tacttgcata aactacgtca
    30121 tccgctctca tagtcggcat atacatatta aagagtaaaa agatacccca aatccctatt
    30181 agtaacaaga tcagttcttt gttattcgtt atttttctat tatgtttgtt catttttata
    30241 ctccttctta tatttcctaa tagagtataa catagcttta agcattactt aaagataact
    30301 taaaagttat cttttaaata cttaattgtt aaatacctta tttgatgcaa aagaaatcta
    30361 cgatagtcaa caaaaaaatt ttcattatat ccagaaaaat ttttcttagt taaggaccta
    30421 tggattgcag taatgcagaa ttcgtcgaat aaactgccaa agacatacaa tcatattgct
    30481 tttaaaattg acgaacaaga tatagattcg tttgtctcaa aaatccaaat gttaggttta
    30541 actgtcgaac caggacgttc tcgtgtgaaa ggcgaggctt cttcaattta cttttacgac
    30601 tatgataatc acctcttcga gttacatacc ggtactctcc aggaacgttt aaaatcctac
    30661 aattccacta cctgagacta gtaatgacta gtctcttttt atggcctctg atccccccat
    30721 tgagcgaata ctaatagggc ttcgaattga tctgttcaat ctttaagcaa tatccctcaa
    30781 atgagtttgg aaagtttagt gattcatcac aaatacttaa atacatgtag ttatatatag
    30841 attgaggttc ttgtgagcgt atgctcgtgt gggttcaaat cccatcgtct gcatccatct
    30901 ttccaaaaca agcggctagg acatttttgt ctcagccgct tgttttctag tttattcttt
    30961 catcttgtcg gacctttttt cttgggtata gacttttctc atattctacc acaattttgg
    31021 gtttccctaa cacatgatag gatctcacaa caaattgctt tctattatta taataacctg
    31081 taacgactaa cttcatccct tcgtctactt cagctaggaa gttcaaacta tgggaagcaa
    31141 tcaaacaatt gaccccattt aaactgaaac ggatcaacgg tctggcacta acttgcaaag
    31201 tacggatctt atctactgtt ccatattttt tctccataac cttcacctca ataccattat
    31261 acgaacatac attccctttg taaagaagaa actctgtttt atctttttga ctttttgtct
    31321 attacctttc ttaaaatcat attggcactt cctgcgaaga tgaaggacat gtactaaaaa
    31381 aaagaggcag cagtatagat tactgttgct tctttactaa tttaggttta cttgttaaga
    31441 aagattattt acgcaaatgt tcttcaacaa tttgctgaga tttatcagct gtatcttcca
    31501 cttcatcttt tgctttttct aacttttgaa tatattgttt caagcttgag ttttcagcag
    31561 ttgcttcgtc taatttcttt tgtgtatctg ttaactgagt ttctacattt gttttttctt
    31621 gtgtcagtgt tgcgatttgt tgattcaaac catttacagt attttgtaat tctgaaagtt
    31681 ttgcttgtgc ttcttgtagt cttgctgtcc aatctgcatt cagatgattg atttcatcat
    31741 ttttttggtt gatctcatct agtttgacat tgatttcatt ttgtttgctt tgtagttgtg
    31801 catccttgct gactaattga ctttctaagt tttgtttttc tgctgccgat tgattttttt
    31861 ggttttctaa ttcatttact tttgtttggt attcttggac atctgccttt gaatcttgca
    31921 atgtctgttc tttttgtgct agtcgagcgg taagatctgt aagcgcttgc tctttatctg
    31981 tcaatgtatg atcaagctgt gtcagattct cattgattgt atcaatatct gtctctccac
    32041 cccacaaaat aatttcattt gcgtaccgcg tgcctgtgta gccaattaga catagaaatg
    32101 caacgacaat cccaccagta ataaatttct ttttcatttg catagctcct taatccaata
    32161 cttttacatg ctaaaagtaa gtgatctcaa acacaatgtc aagcgttttt aaattacatg
    32221 tgtaatttta atataaatac tatcaaatac tcatttaatc ctcctttttt atctatttaa
    32281 aaaatctttt ttatagagaa aaatgcaggt taatactttc ttaaataaat atttatggtg
    32341 aacaaaaaca aaaaagcaag ttttattaaa cgtaaccctt ttcatttcat agattactgt
    32401 atttattaca aaaaacg
//
LOCUS       DARBFO010000021        32207 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000021 DARBFO010000000
VERSION     DARBFO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.022"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(145..525)
                     /gene="yajC"
                     /locus_tag="SCK51_001130"
     CDS             complement(145..525)
                     /gene="yajC"
                     /locus_tag="SCK51_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEG1251770.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(622..1767)
                     /gene="tgt"
                     /locus_tag="SCK51_001131"
     CDS             complement(622..1767)
                     /gene="tgt"
                     /locus_tag="SCK51_001131"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEG1251771.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1985..2683
                     /locus_tag="SCK51_001132"
     CDS             1985..2683
                     /locus_tag="SCK51_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEG1251772.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2851..5490)
                     /locus_tag="SCK51_001133"
     CDS             complement(2851..5490)
                     /locus_tag="SCK51_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1251773.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6109..7140)
                     /gene="queA"
                     /locus_tag="SCK51_001134"
     CDS             complement(6109..7140)
                     /gene="queA"
                     /locus_tag="SCK51_001134"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEG1251774.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7333..8508
                     /locus_tag="SCK51_001135"
     CDS             7333..8508
                     /locus_tag="SCK51_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1251775.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8512..9153
                     /locus_tag="SCK51_001136"
     CDS             8512..9153
                     /locus_tag="SCK51_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1251776.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9150..10067
                     /locus_tag="SCK51_001137"
     CDS             9150..10067
                     /locus_tag="SCK51_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1251777.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10064..10735
                     /locus_tag="SCK51_001138"
     CDS             10064..10735
                     /locus_tag="SCK51_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1251778.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11276..11764)
                     /locus_tag="SCK51_001139"
     CDS             complement(11276..11764)
                     /locus_tag="SCK51_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEG1251779.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11965..12231)
                     /locus_tag="SCK51_001140"
     CDS             complement(11965..12231)
                     /locus_tag="SCK51_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG1251780.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12231..12494)
                     /locus_tag="SCK51_001141"
     CDS             complement(12231..12494)
                     /locus_tag="SCK51_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1251781.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12970..13155
                     /locus_tag="SCK51_001142"
     CDS             12970..13155
                     /locus_tag="SCK51_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1251782.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13297..15120)
                     /locus_tag="SCK51_001143"
     CDS             complement(13297..15120)
                     /locus_tag="SCK51_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEG1251783.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15718..18423)
                     /locus_tag="SCK51_001144"
     CDS             complement(15718..18423)
                     /locus_tag="SCK51_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEG1251784.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18729..19676)
                     /locus_tag="SCK51_001145"
     CDS             complement(18729..19676)
                     /locus_tag="SCK51_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEG1251785.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19866..20135
                     /gene="rpsN"
                     /locus_tag="SCK51_001146"
     CDS             19866..20135
                     /gene="rpsN"
                     /locus_tag="SCK51_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEG1251786.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20311..20673)
                     /locus_tag="SCK51_001147"
     CDS             complement(20311..20673)
                     /locus_tag="SCK51_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEG1251787.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20684..21805)
                     /locus_tag="SCK51_001148"
     CDS             complement(20684..21805)
                     /locus_tag="SCK51_001148"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEG1251788.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21821..22171)
                     /locus_tag="SCK51_001149"
     CDS             complement(21821..22171)
                     /locus_tag="SCK51_001149"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEG1251789.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22301..23812)
                     /locus_tag="SCK51_001150"
     CDS             complement(22301..23812)
                     /locus_tag="SCK51_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG1251790.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24134..25486)
                     /locus_tag="SCK51_001151"
     CDS             complement(24134..25486)
                     /locus_tag="SCK51_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEG1251791.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25639..26715)
                     /locus_tag="SCK51_001152"
     CDS             complement(25639..26715)
                     /locus_tag="SCK51_001152"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEG1251792.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26911..28656
                     /locus_tag="SCK51_001153"
     CDS             26911..28656
                     /locus_tag="SCK51_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEG1251793.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28936..29373)
                     /locus_tag="SCK51_001154"
     CDS             complement(28936..29373)
                     /locus_tag="SCK51_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEG1251794.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29357..30730)
                     /locus_tag="SCK51_001155"
     CDS             complement(29357..30730)
                     /locus_tag="SCK51_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251795.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31052..31540)
                     /locus_tag="SCK51_001156"
     CDS             complement(31052..31540)
                     /locus_tag="SCK51_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1251796.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31562..32164)
                     /locus_tag="SCK51_001157"
     CDS             complement(31562..32164)
                     /locus_tag="SCK51_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG1251797.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9208 a         6883 c         5943 g        10173 t
ORIGIN      
        1 aagttattta ttattattta tatgacacac ccgattattc aatattgagc aatatatccc
       61 atgctcttat ctctttcttt tttgaataaa aaatgaggta ggacatcttt ctgccctacc
      121 tcgtctttaa ataagctttg atacttattt ttcttccttc tcttctgtct cttccagcgt
      181 ttctgttgtt tcagttttta cttcaactgt ctcagctggt tcttctgttg tttcaacagt
      241 agtcgttgca tcatttgtta cgacagtccc tggtttgacc gttttgattg ctgcacgatc
      301 gtactcaagg aagatccctt cgcagtctaa aacaactgtc cgtttgtcat tgtcgatttc
      361 tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt tcatgctatc
      421 taacaagttt tgacgttctt gctgttgttt cttttgcgaa cgtgacataa aaaaccacat
      481 tgctaatagc gcgataaaca tcacaagcat tggtaatgtt cccatttttt tcacctcact
      541 aattctgtat ctacaaaaaa cactttagca gaaaaatgct gataacacta gttttttact
      601 taatttttaa aagattttcc tttagaagct ttttgcgttt tctttattga acccgtattc
      661 ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt gacgtacttg
      721 tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa tcccaaatgt
      781 ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac atgtataaca
      841 atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt tcactactag
      901 acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca agacacagtc
      961 aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac cgacacccat
     1021 caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata cacgattcat
     1081 ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga aatccatccc
     1141 gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt ggatgattcc
     1201 aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg cccatcgaga
     1261 cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg gacattcgtc
     1321 aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt tttctggtga
     1381 taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt cttcgatatt
     1441 ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa tcggttgatc
     1501 ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac cagggcgtag
     1561 ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca actcttctgg
     1621 ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg tggggaaagt
     1681 tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt tcttgattaa
     1741 acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa aatatcttga
     1801 caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg cttccttcta
     1861 ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa agaaaaacat
     1921 tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa aggagggaaa
     1981 aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa aaggacgttg
     2041 gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta ctattcttct
     2101 tattatgatt cctattatct tgtttgccac caccatatat gatggatcag cgagcgtctc
     2161 agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta tttccagcat
     2221 cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt tacgtggaca
     2281 aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt tgtttatcgg
     2341 cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg gtttgctatt
     2401 agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta tttactacga
     2461 tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg caagtcgaaa
     2521 attgatggat ggttataaag gacaactttt ctggttagat ctttcattta tcggctggca
     2581 tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat acatttctgc
     2641 aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag tgtgacttgt
     2701 gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa acattcagcc
     2761 cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt atagttccag
     2821 taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt ccggtaatcg
     2881 atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc ctaccaagaa
     2941 ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg gcagcaaagc
     3001 aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt tattggtcgc
     3061 taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca gaatcgatga
     3121 ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt ctacccatgg
     3181 gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga tggtgaagac
     3241 ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt catttttcgg
     3301 aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag caaatcctgg
     3361 aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg gtgctcccca
     3421 tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc ttagcaaaaa
     3481 gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag cgtctacgat
     3541 cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca catctgttcc
     3601 tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat cattcacacc
     3661 atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga tccggatctt
     3721 gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat ctaattcttc
     3781 gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat tcaagatacc
     3841 taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca tgactgtttt
     3901 gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg gtggatcgat
     3961 cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag aaatatcttg
     4021 cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc gtttaccaaa
     4081 gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg gagcaagaac
     4141 atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa ctgttgtcat
     4201 catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt cttctaattc
     4261 tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg ttggattacc
     4321 tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata gtgcagccat
     4381 tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta cttcatcttc
     4441 tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg ttttgtctga
     4501 gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga tgatcgcatg
     4561 tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa cagtcaaggt
     4621 ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa ggagtggttc
     4681 tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga tcatactgat
     4741 tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag tattttcgtt
     4801 caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga cgatcgcttt
     4861 cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt cacctaatga
     4921 ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt ctccagtcaa
     4981 cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg cagaaatagc
     5041 atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact caacagtggt
     5101 cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca aggattccag
     5161 tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca caacgatagc
     5221 gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg tataaaagga
     5281 aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc gaaagaattt
     5341 tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct tttttcgccg
     5401 ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta ccttctttac
     5461 ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc ttcgtacaaa
     5521 aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa agggcttatc
     5581 gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg ctaccaaagg
     5641 atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag aatttctcga
     5701 tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga catgcgtgta
     5761 gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag tatccgaaac
     5821 aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt gaataggaac
     5881 aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta acatgaagaa
     5941 attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt ttatttgaat
     6001 ccttccgagg tacaagaaag ccgagcttat cgagagctcg gctttttctt tgtcataaaa
     6061 tctttgtaaa gcttttttgt tattcaaaac tagcggcttt tcataacctt atttcacgaa
     6121 catcgcatct ccaaaactaa agaaacgata gcgctcttca atcgcatgct ggtaagcaga
     6181 taaagtcaat tctcttcctg caaaggcact aactaacatg actaaagtcg attttggcaa
     6241 atggaaatta gttgagaaag cttctaccac tttgaattca tagccaggcg taatgaaaat
     6301 atcagtccat ccgctgtctg ctttgatttc tccattaaat ttcgttccaa tcgtttctaa
     6361 tgttcgaatt gaagttgttc cgacagccac gattttcccg ccatttttac gtacttggtt
     6421 caatcgctca gccgcttctt ccgtcaaacg gtagaattca ctatgcatat ggtgttcttc
     6481 aattttatct acgctgacag gtcggaatgt acctaaccct acatgtagtg tcagatagac
     6541 taattcaaca ccctttgctt cgatctcatc aagtaattct ttcgtaaaat gcaatccagc
     6601 tgttggcgct gcagcagaac cattttcttt cgcatagaca gtttgatagc gatctgggtc
     6661 ttccagacgt tctttgatat atggaggcaa tggcatttct cccaatgatt caagaatctc
     6721 tagaaaaatt ccttcatact caaagtcaat gatccgtccg ccgtgttcca attcttcttt
     6781 cactactgct tgcaatctgc catcaccaaa agagattctt gtcccaactt tggcacgttt
     6841 cgcaggtttg atcaatgtct cccacgtatc tccttgtgta ttcgttagaa gaaggacttc
     6901 taaatgcgca ccagtatctg gtttttctcc atataaacga gcaggcagaa ctcgtgtatt
     6961 attcataact agcgcatcac caggctgtaa ctcttcaatg atttcatgga aatgtttatc
     7021 ttccatctct ccagttttcc gatccaaaac aagtaaacga gaatgatcgc ggtcttttaa
     7081 tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca aaatcttctg ttgttaacat
     7141 atcgtcactc actttctatc atgtgcttca tcattctatc atgtttttcg ttctgttggc
     7201 aaaatcagca ctttttcact tctatttttt gttttcctaa ataaaaaaac taccctattt
     7261 gtattttttc attgttttag gcatactagt aatagatagt tcttcattga aaatagaaaa
     7321 agaggtggca ccatgattga atttcaaaat gtgtcaaaaa tttataaagg tggaaaagtc
     7381 gctgtagagg acgtcaatct ttcatttgaa aagggtgagt tcatttgttt tatcggtacg
     7441 agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc gaatgactga tccaacacaa
     7501 gggaaaattt tgattgatgg aaaatccatc caagaaattg atccagtgga attgcgacgt
     7561 cagatcggct atgttatcca aagtattgga ttgatgcctc acatgacgat ccgtgaaaat
     7621 atcactcttg tccaaaaatt gttgaaagtc agtcaagaag aacggaataa aacagctgaa
     7681 aaaatgattg atcttgtgga attgccccgt gagatgttag atcgctaccc acatgaatta
     7741 tctggtggtc agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa tcaagatatc
     7801 atcttgatgg acgagccatt cggtgcgctt gatccaatca ctcgtgattc cttgcaagac
     7861 cttgtcaaag atttacaaga gcgtttggga aaaacgatcg tatttgtaac ccatgacatg
     7921 gatgaagcga ttaaactagc aagccgtatc gccatcatga gtgaaggccg tgtgatccaa
     7981 tttgatacac cagaaaatat tttacgtcat cctgcaaacg atttcgtaga agagttgatt
     8041 ggagaagatc gcctactaca agccaagcca aatgcgacaa gagtcggcga agttatgcta
     8101 aatacagcaa tcacgattac accggaaaaa tcattacaag aagcaatcaa actgatgagg
     8161 gaaaaacgtg tagatacgtt gttagttacc gataattcga atgtattaaa aggatttatc
     8221 gatgtagaaa cgttgaataa aaaacgagga aaagtatcca gtgtcggcga cattttgaat
     8281 aaacatgttt tttacgtaaa agaatccgcc tatcttcgtg ataccttgca acgcatttta
     8341 aaacgcgggc tgaaatacgt gccggtcgtt gatgaacaga acaaagtcgt aggtatcctg
     8401 actcgtgcat cattagtgga tatcgtctac gatgtgattt ggggagaaga tcccgcaact
     8461 actgatgttg cagagtcagc tactcctgaa gaatctaagg aggtctgacc tatgcagcag
     8521 ttttttcatc agcacggttc agaaattctc tcaaaaggtt ttgaacatct ttatatttca
     8581 ggacttgctt tgatcattgg tatcatcatt gctgtaccgc ttggtgtctt actgacacgt
     8641 tttccaaaag ctgccacgat tgtgattggc ttaaccagtg ccttgcagac aatcccttct
     8701 cttgctttat tggcattgat gatcccgcta tttggcgtag gtaaattacc tgcgattatc
     8761 gcactcttta tttattcatt attgccgatt ttacgtaata cgtacattgg gatgaaaaat
     8821 gtcgattgga attatcggga tgtggcaaaa ggcatgggga tgactgagtt ccaatccatt
     8881 ttttccgttg aattgcccat tgctatgcca acgatcatgg ctggtattcg tttagctgcc
     8941 gtttacgtga ttgcatgggc tacacttgcc tcttatatcg gcgctggtgg tttaggcgat
     9001 ttgattttta gtggactgaa taattaccaa ccagatctca ttttggcagg tacgatccct
     9061 gtaacaattt tagcactatt ggctgattgg cttctaggtc tcttggaaca ccggctgaca
     9121 ccgatcgcac ttagagagga gaatgaagaa tgaaacgtat actaaaaatg accatggttc
     9181 ttttctctct tcttatactt gcgagttgtt ccttccctgg tcttgccagc aatacagatg
     9241 aagacaccat ttcgatcaca ggtggaatca cttcagaagc acagatctta gcaagtctag
     9301 ttgccggaat ggttgaacat tatacagata aaaataccgc aatcatcaac aatctggcta
     9361 ctacaactat caaccatcaa gcgatgatga acggagatgc ttcaatttca gcggctcgct
     9421 acacaggaac agatttaacg actacattga accttcctcc tgaaaaagat ccgaaaaaag
     9481 cgtttgctac cgtgaaagat gaatttgaga aaagatacgg tcaaacgtgg ttcccgtcct
     9541 atagttttga aaatacgtat gtctttcttg ttcgaaaaga caccgcacaa aaataccatc
     9601 tatctaaagt cagcgatctg aaaaatgtag cggatgaatt agtagctggt gtagatactt
     9661 cttggatcaa tcgtaaagga gacggttatg acggcttcca agaaacttat ggcttctcct
     9721 tcaattcgat attacctatg cagattggac ttgtttatga cgcagtcgaa gcaggaaaaa
     9781 tggatattgt tcttggctac tctacggatg gacggatcgc cagttatgat ctagtcatgt
     9841 tggaagatga tcgtcaattc ttcccaccat atgatgctgc tccagttgta gacaacaaga
     9901 tattagctga tacgcctcat ttaaaagaag ccttaaataa gttaggtcat accatcagta
     9961 cggaaaaaat gcaggaattg aactacgaag cagataataa tctagtggaa ccatcagtag
    10021 tagcacaacg tttcttacaa gaaaaccact attttgaagg gaagtgaagc aattggataa
    10081 aatgaatgtc tttcaacaat ttttctatta tttccaggaa aatggctcct atatctttgc
    10141 acaatttatc cgtcacttcc tgatttctat ctatggtgtc ctctttgcag cagttgtcgg
    10201 gattccggtt gggatcatga tttctaggag acgtaaatta gcaaactggg tgattcgttt
    10261 ggcgaatatc atccagacga tcccttctct agccatgatc tccattttaa tcattggatt
    10321 aggcctggga gtcaatgtcg tgattgttac tgtatttttg tattccctct taccgatcat
    10381 caagaatacg tataccggga tgatccaagt agacaaaaac atccttgatg tcggcaaagg
    10441 catggggatg actgctagac aacgactatt catggtagag ttaccactct ctgtttctgt
    10501 tatcatggca ggcataagaa acgcgttagt tgttgccatc gggataaccg cgatcggtgc
    10561 attcgtcggt gctggtggtc ttggagatat cattatccga ggaaccaatg caacagaggg
    10621 cacatcgatt attttagcag gtgcattgcc aactgcatta atggcgatca tcacggactg
    10681 gcttttaggt attttggaaa gacggctaga tccagccagc cgtacatcac gctgatgtta
    10741 ttgtaaaaaa caaaagcttc tgcgaccatt tgttggtcgc aaaggctttt ttagttctgt
    10801 catttttaag gcaataatcc actctgattc ttccctcctt atgggtaaag agttgctttt
    10861 cttagctatt tatccattcc gtatactcca acttcatact gccaacgggt tcatatctgc
    10921 atccaccgac taagtttttt tcttatccgc taaggcattt tgccaatcca ttttcccatc
    10981 actcgtcatg gaattcatac cacatgatct ctaaatactc gtctgcttgt tctttgaatt
    11041 gggcatcaat tcttacatcc tttggcgtgg ctttcattta agccttttcc atcagtcctc
    11101 gattgttcga tgccttcagc tgcatcatca aaactggtga ctgccgtatc ggtaacttcg
    11161 atcatctttt atacaatcgg gaacttgcca tcctcatcct tggctaaaaa catatctagc
    11221 cattccagct tctcgccatg ggaggtttgc ggttcctaaa accggtacac gattcttatg
    11281 gactgcatcc gtcgcaacta gacttggcga tacgatgatc cttcaccaga agagcacccc
    11341 cgtaaaccaa ttgatcgata tattgccaat aagaaaagac attggcgtaa aagatatcga
    11401 tccctcgtga tgcattgccg ttgatactgc tattcatggc cgaaagcgcc acgactttaa
    11461 cttcttccgt ttgagtgaca ttcatgttcg gcaatgtcgc tttggatacc cgctctgcta
    11521 aaggaacgac actggtattg tatttcgtat ctgtatcttt tccaaaagac catgccaacg
    11581 aatcctctgg gaaccaataa gacgactccg gttgattgtc cattgctttt ttcaacatac
    11641 ttttggctgt ttctcgataa acgacatttc caattgtctt cgcctcactt tggcgataat
    11701 cgatggtcgt tgccactgcc aacactaaca acgatgctcc aattttactg attttccttg
    11761 tcacacttct tcctccttgt tttacataaa cccgtcaaca ttccattcct aaactcaaac
    11821 aatgaccctt caaaggtgga gatataggaa caaattaaag aaatcatgca ataaatccaa
    11881 gaaggttggt tttagaacaa aaaaagagtc tcctttttgg ggagactctt tgttaactat
    11941 ctaaaaaggt tggctcttct cacttcactc ataatgtgtc caggcagaat aaatttctac
    12001 aactttattt tcctcatcaa ctttgtaaac cactcgatgc tgtctattca gcctgcgtga
    12061 gtatctgcct tcatgtgtag gtctcagctt ttcaaaagac tgcgtgggca tatatggatc
    12121 ttccttcaat gtctgaatca cttcttcaaa ttgtttcttt aaattcgttt gcttgatttt
    12181 tcttaaatcc actttcgcag agtttttgat tgcgactgta tagttactca tcaaagagct
    12241 tcccaatcaa tatcatcgat attcgtaaag ctactgtcgt ctttttcgcg gtctcgtact
    12301 ttatctaaag tacctgtttg ctcaagaaaa agggtttctt gaatcgctct ccagtcttcc
    12361 agcccaatca atacagcatt gtttccacta cgatcactaa taatctcgat ttcagtatga
    12421 ttttcattta cctctttcaa taactgataa aaatttttac gagcagtgct aggattcatc
    12481 gttgtattag ccattgtctc tcctcctttt tctgatataa ttatagcgta cttgattaag
    12541 tacgtcaaaa aaatacaata aagcacttaa attcagcaaa cttttcctct tttttacata
    12601 gagaaaaaga ccccttttta gggaggatct ttgtccacgc tcacttctta ctcctttacg
    12661 actagcggta gccaaacgta aagtaattgg taacaacgta tcatctttag caaaggtcga
    12721 atccaaagcc tgtgagtcta tgcaacaaca ccaatacacg aaggttcctc gttgcagtgt
    12781 gacttgattg atatcttgaa taacctgagg atacgcacga aagatttgta acggttattc
    12841 aaaactgatt ttggccgtca agtcttctac tgcctcaaaa atgattgcct ctttcgtctc
    12901 ctgtcattca tgttcactat cgatttgata cttgcttaga ccgttgggtt tgtgaatcac
    12961 caattaaaaa tggcactgag ttatggtaac aaaaaatggt cacttgacga ggctgagatt
    13021 cctacttatg aagacacatg gcacttaaga gtcgaggtaa aggaagaagc tgaaaatata
    13081 ggaggctgta cattatttgg caaaggattc ggaaattatg atcaagacat caaaatcaaa
    13141 atgttttaca gctaaatagg aagaataata acatttcata cgctgttccc atctttaatg
    13201 gcttatagag ggcaccttta gctgagaata agacaaaaca gaccctctag cttcaattaa
    13261 gagctagagg gtctgttttt gattttaaaa cttggtttat ttcttcaaat ggtagctata
    13321 ggtgctgaca acgatatttt cacgccaaga caatcgcaat cttaaccgca aacttgtttg
    13381 gttatggaga atattctgcc agcgtctgtt tggtacaaat tgaggaatca acacagtagt
    13441 tgtatagttt tgtttcgctg catttttact tacgatatct acataacgga taattggatt
    13501 ttcaatcgag cgataagaag agtggacgat tgaaaaacgg acatctggga aatgcttttt
    13561 aaattctgct tcgatttctt tttctttttc gacatcttcg tctagcgaaa catgcatcgc
    13621 aacgacataa tcaccgatcg aacgtgcgta attcaacgca ccgatattta cccgtgtcac
    13681 attcccgact aatacaagaa cggtattccc gtcataatca tgtagttttg catcattttc
    13741 taaacgaagc tgttctgcta cttttttata atgatcatgg atcttataaa aaatgaacat
    13801 aacgattggc ataatgatga agaaaggcca aatgtcccct aaccgataga caaacaaaat
    13861 cgcaatgatt gcatatgaga tgaacgcacc cgtaatattg gcaatcgatt tgcttaacca
    13921 tctttttcct tcttttttcc atttgacgac catccctgtt tgagataaag caaatggaat
    13981 gaacacgccg atagagtata acggaatcaa gcgttctgtt gagccatgga aaataaacag
    14041 caatacgatc gaacctagcg ctagtgtgat aatcccattg gaatagccta gtcgatcccc
    14101 acgatcttga tacatgtgag gcatgaattt atcttttgcc agattgtatg caagaactgg
    14161 aaaagcagaa aaaccggtgt tagcagcaac tgctaaaatc agcgctgtag caaattgaag
    14221 gacgtaatac aaaataccat gtccaaagac agctttacca atttgagaca aaacagtgac
    14281 ttctttttca ggaacgatcc cataccaata gttgataaat gtgatcccca cgaagaagaa
    14341 acctaagatc aatgccatca tcgtcaatgt agcagcggca ttttttgctc ttggcttctt
    14401 aaagaatggt acagcattac taatcgcctc caccccagtt aaagaggaag aaccagaaga
    14461 aaatgctcgt aaaatcaaag cgatagaaac acccggcaca gccgctcctg gaagtgcagt
    14521 agcttgcaat ggcacagcac ctgtcacgat attatataaa ccaacaacga tcagtaacga
    14581 gatcacaagg ataaatgtgt acaccggaaa taataggaac gatgcagatt ccctcagccc
    14641 acgaagattc agcatcatca gtagcaaaac gattgtcaca gaaatagcga cttgatgacc
    14701 gtagagtgct ggcaccgctg atgtgatcgc ttcagcacct gctgagacag atactgctac
    14761 tgtcaacatg tagtcgatca ataaagagcc accggaaatc aagccagcat tctttccaag
    14821 attctcactg ctgacgacat atgctccccc gccatgagga taagcatgga tgatttgacg
    14881 atatgatagc gttaatgaaa tcaataaaat aataacaaaa gcggcaatgg gtagcgaata
    14941 ccagatggct gcagcagaca aagcgactaa aacgacaacg atttgttccg taccatatgc
    15001 aatagatgat aacgcatcag acgacaataa tgccaaagca gcaaatcggg ttaatttgtg
    15061 ctcatcattt tcagcagatt ttaaaggctt acctactaac aaccttttta agtaatccaa
    15121 tttctaaact cctttacaca caattttact tcaagtttta tagtcgaaaa tttatagtaa
    15181 acaagatgtt atagtacgct cactgacctt aaaagtcaat aaactttaag aaaataattg
    15241 gaaaaaaaca gaaaaatatc aagcgctttt tcaaacgctt taatacagca aaaatctaac
    15301 atttttttat gggctttttc tttgaattct ataataaaag gatagaactt gacttagaaa
    15361 atcaagttct atcctttttg atatcttaac ttttggaaga gagatgtaag gctgctttct
    15421 acccatcatt cttactactc gaagcatagc gcagttgtca caacctcttg ataaacggtg
    15481 tttaaaagct tgcatctgcg gtaataagcc caaccaaatt aaaaacataa gaagctgttt
    15541 ttatttggtc gttcttatta cttgatgcag agcagctttt ttacaacctc ttggtacacg
    15601 gtgttcaatc agctgaccct cggtattagc tcaaaaaaac ggcaaaatat gaggaactat
    15661 ttttccattt ttttctctaa tactcaggtc aaaacactga tttcacaacc tcttttttta
    15721 atgaaaaata tggctctcat gcgccgcttc tttgaatttc gtcatttttt cttcgatgaa
    15781 cggatagatt gccgttccta cgattccaaa gacaacaaac aaaccgatca tgatccagat
    15841 tgctttcgta gcatttggcc aataaattcc tcctgcagat tcccgtaaca agtttaccgc
    15901 atgagtaaat ggcaagaacg gattgatcat ttggaagaat ttccctgaga cttggatcgg
    15961 atagttcccg cctccgccag agatcgacag tactaagatg ataatagcga tccctttacc
    16021 gacattatcg aataatgcag ccaagacata gacgatcatc atgaacgcta aggcgatcaa
    16081 gacagcaaat gacacgctgt agaccggatt ttggacatct actcctagta agaaaatatt
    16141 ccctaatgta acgatcaagc tttgcgcaat cgccattgtc aaaaatgtca gcatacgagc
    16201 aacgaattgt tctcgtttcg taaaattgtt tcggtctttc tttcctaaat agtattctgt
    16261 cgtagcaaca cttgaaagaa gaacagcgcc gacccacaga catagtgcag tataaaacgg
    16321 ggtgctagct gaaccgttat ttgcgattgg ataaagcgca ttggtttgta gctcaacagg
    16381 ttgtgtaaag aagtcacttt cttttgttgc gtctagtttc aataatttga tgacttcgcc
    16441 tagatcgact tcttgttcgc cttttcggat cgcgtctgct gctttttgga taccagcttt
    16501 gatatttggc cagtcattgt tgatcaagtc cgcagcagcg ttcacagctg tttcgacttc
    16561 tggcattttt tcatttactg ttgtcaatgt accggtgatt tcatttttta gtgctggcca
    16621 atcattttgg ataaaatcag cggccaatcc taatttttgc tgaatgatcg gtaattcatt
    16681 gttgtatagg tctgcccctt cattgatgcc attgacgatg gtatccatat ttccattcag
    16741 cattgtattg gcatcatgga tttcttgtcc aattgcaggc atttcttttt gatatttttc
    16801 taaaattgcc actgcattcg ttacagttgc tttcgttgag ctgagaagcg aatcaaagtc
    16861 gatctggtcc gctttgctca gtgcgccttg tgcagtccca atcgtatcga tgacttggtc
    16921 tagtagagaa gtgacagtgg aactgatttg acctgtattg ataccagcaa tgatgccatt
    16981 gatattatta acaacggcat caatttgatc tagcaattca tcaatagcct tcgtgtcttc
    17041 attttgaaca gctgtgttca aagcagatag gcggttttgc agattgctta gcaagggttt
    17101 taattgattt agctgatcaa taattggctg taatttcgta tttccagcac ttgtttgcaa
    17161 tttagtcagg aactcgacca gtttatcgat tgcattttgt tgttgagata ttccgtcaat
    17221 aaagccgttc aatacattgc tgatgttttt cttttcatca tcagtcagct taccatcttc
    17281 aatagctgtt cttacagtat ctattaacga acctgtcgtt gttgccactt gtccaattga
    17341 cttcaatgta acatctacac tgtttgtgat gcttggtatc gcatctttca gctgagaggc
    17401 acctgatttt gtttggtttg ctagatcact tgcctgattt tctaaatttt tcacatctgg
    17461 caagatatct tgtacttgtt ggatgatcgt caagccttgt tttgcttcat cgattccttg
    17521 gttcattgtc tcttggatcg aagcaaaatc gccgtcgatc tctgctagtt gcttaccggc
    17581 attttggatc tctgggattt tttcttgcaa atcaaggatg atttttgctt gatctttcaa
    17641 ctcaggcatt ttgtcattca atgcaacgac tttttcaccc atctcatcta ctttagggat
    17701 gtaatccacg aattcatttg ctttattcag tttttccttg atttcaggta gttgactttg
    17761 taattcgagg acttgttttg tgtagccgtc gatcgtgtcc aaattttcat ctgttgataa
    17821 aatcatatct ttaactttat tgatacttac taggttcgta tcgatgtcgt aacctatctc
    17881 attgaatact ttcaataaag tcgaactggc tgttttgata aattcatttg tgatttgcga
    17941 ttggatcgaa cttgctcctt tatcggtgat tttcggtgca atcgcattga ttttttcatt
    18001 taccgtatat tcgatttttg gtttcttgat atcaccacta gtaaaactca gcagatcttc
    18061 tgagaaatcc tttggcaagt aaatacctgc gtaatacttt ccggaacgga ctccgtcttc
    18121 taattgttct tttgaatcaa cgaattgcca tcctaattgt ttatttttat gaagcgattc
    18181 gatgacttgc tccccaatgg ccacttcttt accttgaaac tctgctggct tgtctgcact
    18241 gtaaacagca attggcagct ctccagtatt tccatatgga tcccataatg ctttgatgtt
    18301 aaaccacgca tatagcgaag ggatgatcat gatcgcgatg atcagaagtg ttgcaatcgg
    18361 gtttttgaaa atccgtttcc agtcaagcaa atataatcga agcgtatttt tgattgactg
    18421 catagttctc ccccttcttc ttttttcagc tgaattactt taccatgtat ttcatacttt
    18481 tttcaatcgg cttttttcaa aaaagtcgaa tttttggaca tatcacggca attgtctcat
    18541 tgtaatctct tacatattga gaaataatta aggattacag cctttacttt atcatgaaat
    18601 aaaagggaaa gataatttta tgccaaagtt tttatctatt atacgtatac gcaaaaaagc
    18661 taaaacattt tttgtctttg cccctacaaa aatgtttcag cttttttcgt tttttattcc
    18721 tgtatttctt atttttcgaa tgacaggatg attttcccag caggaggatg tccttcaact
    18781 agctgatgtg cttggatgac atgcttaagt gaagcagaca gattcattga aataaaaaca
    18841 tgaaaggctc ctttttggta ggctttgatt atgccagcaa aggcttctgc atctagatat
    18901 tcctttcttg gaacaaacga ttcataaaag ccttcctttt tttgccgcaa atctagatct
    18961 gggaggtcat ttaatgcaac atacctgccg cctggtttca tgatctggat gccggtttct
    19021 cctttgatac ttcctttcgt cgcatcgatt acaaggtctg cctgatcagc aaattgcaga
    19081 cctggatttg ttttatcata agcagcaaat gctgaagctc ctaatttttt taccttttcc
    19141 tcatttttac tggatgcact ggttaagatt cggattccct tttcatggag aagctggatc
    19201 agacttgacc cgaccgcacc tgatgctcct agtatcatta cagtatctgt cggctggatt
    19261 tccgtcaaat ggttgattag attataagcc gtaattcctg gcgtgaccat tccggctgct
    19321 tcttcccaac tcattttatc tggaattttt gctacttttg ctgatggcaa cacgacttct
    19381 tctccgtaag tcccgccgac tgcatggaca gctactcgat cacctacatg aacagttgtg
    19441 acatctgaac caatctctgt cacaatacca gcgccatcat tacctggtac ataagggaat
    19501 ttcaatgttc gtatctcttt catttctcct aaacgcaagg caacatcata aggattcaca
    19561 ctgaatgctt ttatttctac acgaacatgt ccagaaccga cttctctggg atgagcatcg
    19621 atttcttcaa aaacatcttg tgcttctccg taaccgttaa ttgcaaatct tcgcatggtc
    19681 accatctccc tttttcttta agtttacgtt tatgccttta ttttgacaaa aaaaatgtct
    19741 tttcgttttg gcgcatgatt caaaacaaaa tagttgtctt ttttcgcgta acgtgttaaa
    19801 ttatttatct aaaagcgtaa agattacgat ttacgctaat aaaaaataat tggaggaatt
    19861 tgttgatggc taaaaaatca aagattgcca aagcaaaaaa acagcaagaa acgattgaac
    19921 gctatgctgc tttacgccag gaattaaaag cgcaaaaaga ttacgaaggc ttgcgtaaac
    19981 ttcctttaga tgctcgtcca gaacgattga aaaatcgtga tttaatcgat ggacgccctc
    20041 gcggatacat gcgtaaattc gggatgtccc gtataaaatt ccgtgaatta gcacataaag
    20101 gtttgattcc aggagtaaaa aaagccagct ggtaatccaa gtctggctgt aatgtaaagg
    20161 aggagggcgc atggaccact ccttggatca tcaaaaaaga tacgttcgct tcttctagca
    20221 gaaatgctgg tactagaagc agacgtatct ttttttacgc acagctggtc cttttttata
    20281 tgtaaaggct gcatggttac ttcgctatat ttatttagta acaggagtca gtgcttcgat
    20341 acccacacta attttcaatg ctcggtcaac ttctgccata atgggtcgat ccaaatggca
    20401 gaccttttct ttcaatcttg atttatcgat cgttctgatc tgctccaaca ggataacgga
    20461 gtctctttct attcctgttc cagtagcttt gatcccaatg tgagtcggaa gcttaggttt
    20521 agccattttt gccgttatgg ccgcaacaat gatcgtcggg ctaaaatggt tacctagatt
    20581 attttgaata accaaaactg ggcgtgttcc gccttgctcc gatcccacga ctggagaaag
    20641 atcagcaaaa taaatatctc ctctttttac cataggatcc tccttattct tgatattctc
    20701 ttggtatccg ctgtcctaat ccgcaagcca cttcatagtg gatcgtacct aattgatctg
    20761 ctatatcctg catagtgatt tctttgtctg catttttgcc gataagcgtc acttttgttc
    20821 cgacaggaaa ttcttgaggc aatcggatca tcagttgatc catacataca cgcccaatcg
    20881 tttcacagta gttcccttct accaatagag aaaaaccctg catctttcgt ggccagccat
    20941 ctgcataacc gatcggtatc gttccgatcc actctgcttc aggagtgata tatgtttctc
    21001 catagccgat tccttcacct gctgggagtt ttttgacttg aatcaattca gaaaccagct
    21061 caagtgctgg ctgcaatgga taaacttccg gtagtgcgtg cccagaggga ttaagtccgt
    21121 acattgctac tccataacga atcatattgc cgattgtttc atcatgccat aaagccgttg
    21181 cactattgct gacatgtaca tatcgaggaa gttcgggaag ttttttcagc acttctataa
    21241 agcgttcttt ttgcagattc caatatgtat catctgcctg atctgctgta gaaaaatggg
    21301 taaataaacc ttcccattct aatacacgac tttctttgat gaatgcagct gcctctttta
    21361 cttcttcagg tgtacaaaat ccgatacgac ccatcccagt gtctgctttt agatgaatcg
    21421 aaagaggcgt ctctagctta cttttctcta aaacagcagc tgctgcttgc aaccattccc
    21481 gtgtacctgc agttactgat aaatcatgag caacagctac agtcagtgcg tcgtaaggaa
    21541 ccatattcaa gataaggatg ggctgagtaa atccagcttc tctcaactcg actccttcat
    21601 ctaaatttga aacacaaaag cccgaagctc cacctgcaac tgctgcctca gctgtttcaa
    21661 tagctccatg accgtatccg ttcgctttta ctactgcaaa caattctttt ccttgcggaa
    21721 gtcttgcaac ttcatttgct acattttcag ttatagcttt tttatgtatc actgctttcg
    21781 ttgggcggtg ccaagcgaca accatcgttt ttccttcttt ctagtcttcc aaaataattt
    21841 gtgcaaaagc tgtttcattt gtatgagtaa tactgaccca tacattgcct tcatgaggtg
    21901 aacgcgtcac aacaggctga ccattttcat tttttaaaat ctcgacatct tgaaaggtaa
    21961 cttttccgat cccagttccc caagctttag agaaagcctc tttgcaagcg taacgcccgc
    22021 ctaaaaattc tacttggcga tgaaaaggca aggactggaa tagtttcatt tcatcagagg
    22081 tcaagattcg tgcaatgaat tttggttttt cttcgatcag ccgagtgatc ctaggaagtt
    22141 cgactgcatc gatcccaatt ccttttatca tatgcttttc tcttttcaat aaatttagaa
    22201 cactctcatt ctaccaaatt ataaccattt tgccacaaat aagtccacaa ataacaaaaa
    22261 tgaaagtttc tcttaaaaaa gagaaacttt cattttatgt ttaatcgtta tttgaacgga
    22321 ttacaaatcc gcgtttttta tcattgtgac gtttaccgcc atctttttgg ctgttattat
    22381 ttttgctata accgcctttt gatttgtttc cacgataatt accgccgtca cgacgattac
    22441 ggttgtttcc gccaccacgt ttgccatttt tattgtatcc ttttttctgc attggcaatg
    22501 ggcgttctgg tgtgatcttc actggcactg catcagctgg atcttttgct gtcgttttta
    22561 gcaaaagtgc aactaaatct tgtgcagaat attcttctaa taatttatct gctgtttgca
    22621 ggtatttatc tagaccattt tctgcaagtt ttgtttcgat ttgttcaact gcagcaccta
    22681 attgaccttt gaatgcttct ttttcggttg gtggacgtaa agtcgtcatc cgttttttcg
    22741 tcaagttttc aatcacatgc aagtagtcca tttcatttgg tgtaacgaaa gtcacagaca
    22801 taccgccttt tccggcacgg cctgtacgtc cgatacggtg aacgtagctt tctggatctt
    22861 gagggatatc atagttgtac acatgagtga cacctgaaat atctaatcca cgtgcagcaa
    22921 cgtctgtcgc tacaaggata tctaaatgac cgctcttaaa tgaacgcaat acgctcattc
    22981 gtttttgttg agacaaatcg ccatggattc cttctgcacg gtaaccgcgt gcttctaatc
    23041 cgcgtgccaa ttcgtctaca cggcgttttg tacgtccgaa gacgattgtt aattcaggtg
    23101 tttgaacatc aaacaagcga gtcatgatat cgaatttttc gtattctttt gcgcggacat
    23161 agtattgatc gatcaagtca gctgtcattt ctttcgcttt gatcttcaca tgatgtggat
    23221 ttttcatgaa tttaacgcca atattcttga tagctggcgg catcgttgct gagaaaagta
    23281 atgtttgtct ttgatcagga acttgtgaaa tgattttttc gatatcttcc aagaagccca
    23341 tattcaacat ttcatctgct tcatccaaaa ctaatgtttg aacggttcca agtttcaatg
    23401 tgtggcggtt aatgtggtca agcatacgtc ctggtgtacc cacaacgata tgtggacgat
    23461 ctttcaagcc acggatctga cggccaatat ctgctccgcc gtaaacggct tgtacacgta
    23521 cttttttgtc gcgccctaaa cgatataatt cttcttgtgt ttggatagct aattcacgtg
    23581 ttggtgcgat aactaaacct tgtaattcgt gacgatctgg atcgattttt tctaacattg
    23641 gtaatccaaa tgcagctgtt ttccctgtac ctgtctgtgc ttgtccaata acgtcttttc
    23701 cagctaatgc tagtggaatc gtttctgctt ggataggcgt tgcttcttca aaaccagcgc
    23761 gttcgactga tttcaacaat tctggtgata attctagttc tttaaatttc aaatgaatcc
    23821 tcctatatgg ttgttaactg aggatgtagc ttttttgcta ccccgtcgat ttgttcggtc
    23881 aaagtgtatt gtttcttttt gacagtggcc acgcacgttt ttatccattc ttagcccctg
    23941 tggcaccacc gaaaaatcac tttgcgaagt ttaggcatta gaaaagccaa acgggtcgcg
    24001 ttcagcttta tcattattat gacatctggc ttcaacaagt tgtttctatg gcaaaaccag
    24061 tccaatttct cttcatcttt cggcaatagg ttattctaac atagtttttc gttttctgca
    24121 agtttacaga tttttatttc atttgctgaa gttgttctac cacttcaaat aagcccatgc
    24181 tgttgctgcc ttttagcata atactatctg ttgcttcaag attttgtttg atagcatcaa
    24241 tcaatttatt cttttctttt tcaaaataga acacaggtag ttgggggtat ttttggttca
    24301 attcttcttt tagtgcatgc atttgttccc cataaaggaa aatcatggcg aatttctcag
    24361 gatcgatatg ttcactcatt tgcgcatgca tatttaaaga ctctttccct aattccagca
    24421 tatctgctaa cacagcgatt ctgcgtcctt ttgttggcag atttttaaaa gtatctaata
    24481 ccagtcccat ggctgttgga tttgcattgt agacatcgct caaaatagct gcaccatttc
    24541 ctgccgttag ccattcggtt ctgttttgag tgatagaaac atgggcgagc ccttggcgta
    24601 tctcaggtat ggacagtcca aagaagcgcc cgatcgtata cgcaatcaaa gcattcgtta
    24661 cattataact tccaggcagc gggatcgtaa atgtttctcc ttctactaca aaatcggttt
    24721 gtgcttttcc ttttgctgtc acttccgcat gaacatcgcc atttccaata ccgaatgttg
    24781 tgactgtttg tactaatgtt gccgttaaag gtgtcagcaa cggttcatct gcgggcacaa
    24841 cgagtaatcc gtctgataca agtccgtcag tgatttccat tttggcttta gcgattcctt
    24901 ctcgcgaccc aagattttct atgtgtgctt caccgatcat tgtgattgct gcagcttttg
    24961 gttgagccat aagtgaaagt tccgtgattt cattggcgtg atccatgccc atttccaaaa
    25021 cgatcttttc tgtatcttgg ggcatgtgca aaatcgtgta aggaagacca atattattgt
    25081 tgtaatttcc ttgcgtctta tatgttttgt atttttcaga caagacagac gcggttagat
    25141 ctttcgtggt ggttttacca ttgcttcctg taattgcaac gacttctgcg cctacttttt
    25201 ccaagtaata ggttgccaat tgctgcatgg ctttcaaagg atctgctaca ggaataactg
    25261 gaaaattttt tggagcttgt tcaacaggca agctccaaaa tgaagcagat gctccgtttg
    25321 tgatcgcttg ctggataaat tcatgtccat ctcgcgtacc agcaagagga acgaataaac
    25381 ttccttcggt tatcttccgg ctgtcaaatt cgacattggt aatctttgtc agtgcagagc
    25441 attcgctgcc cacggcttgt gcaatttctt gtattgttag attcattgag atctctccta
    25501 aaaaaggttg tgaaaaaagc gtttggccta aacggcgggt tcgcatgact ctcacttcca
    25561 agtaaatttc tctattcgta tttagaagct agaatgaacg cggttacgca atcactccag
    25621 cctcttcttt atttctcttt attcattttt ttcaaaaaaa gattgacgct gatggtatcg
    25681 attcattcct aactggatca gttcttcaat caaatcaccg tatttcaagc ccatattttc
    25741 ccataagagt gggtacatac tgaactccgt aaatcctggc atagagttta attcattcag
    25801 gaataattca tttttatttg tcaaaaagaa atcgcaccgg ctcaatccgc ttccacctaa
    25861 catcgtgtaa gctaacttcg cgtactcttg cgctttttga taaacttctt ctggcacttc
    25921 ggctggaatc tgcatttcga ttttattatt gatatatttt gcttcataat catagaatgc
    25981 tacgtctttt acgacttcac caggcaaagt cgtccgaacg tcttcatttc ctaatacagc
    26041 aacttcgatt tcgcgcgctt caattccttg ttcaacgatt gctcgagaat catactgata
    26101 ggctgttgct aaagcatttt gcagctcttc tcggttttct gcttttgtaa tgccgacact
    26161 agaacccata ttcgccggtt tgacaaacat cggataaagc aaagaacctt cacattgatc
    26221 aaatactttt ttaggatttt ctttccattg attcttaagt actggtacat aaggaacttg
    26281 cggcacacca gcagcttgta aaatatactt ggtcatgatt ttatccatgg cacatgcact
    26341 ggtcaatacg cctgcgccga cataaggcat attcaatgtc tctaagaagc cttggatcgt
    26401 tccatcttcc ccgtttggcc catgtaaaac tggaaaaacg atggctcctt cttctttgat
    26461 ttcgcccgga ttgatcactt ttcctgtaaa gccttcctct gtctgtccac ttgggtccca
    26521 tgaaagatgc aagacatctt tgctggcagg tttttctgtt agtaatggac ctttgaccca
    26581 ttgtccttct tttgtaataa atacgagttg aacttggtaa taattataat aaatggcatt
    26641 taaaactgaa aatgcggaaa gaatggacac ttcatgctct gcgctgcgtc cgccatatag
    26701 taaagtaatc ttcaaaagat tgtcctcctg tattctaaat cgttcagtga tttactctca
    26761 tattttagca caaaacaaaa ataaacgaat aacttattta taaaaaaacg agccagcttc
    26821 taaaagaacg agcgtcattt attttttgtc agctagatta tttgtcccgt tttttataaa
    26881 aagttatgat aaagaaaagg agggatgatg atggcgcaaa caaatccacg cagccgcttg
    26941 gcagaaatca ttcgagtgtt catcaattac aatgtagtgc caaactttgt acagcaaaaa
    27001 aatcccgaac aggtaaaaaa agcctttgaa gaactaggcc ccaccttcat caagatagga
    27061 cagatgcttt ctgttcgaga agatttgctt tcttctgcct tcactcaaac attcaaaaca
    27121 ttacaagata gtgtacctag cgacacattt tcaacggtca aaaagacgat cgaaacagaa
    27181 ctttctcttt cattatcaga tatattcgat gatttttcaa aatcgccttt tgcctctgct
    27241 tctatggggc aggctcatcg tgccaagctg aagaacggtg acgcggtagt cgttaagatc
    27301 caacacccta acatcgccga agagatccga cttgatcttc aactatttga acgagcgatc
    27361 cctttgatca aatacattcc tgaaactagc gtagttgatc taaaaggggt actccaagaa
    27421 gtaaaacgtt ccttgatcaa tgaaatggac ttcttaaaag aaagtcaaaa tggcgaacaa
    27481 ttctatcaaa agaacaatgg ctggaaagag atccgcagcc ccaagattta tgacgctttt
    27541 tgttcgaaaa aagtgatcgt gatggaagaa atgtctggta aaaacttgaa tcatttaatg
    27601 aacgccgaaa acaaaactga aacttttatc actggaattc aaaacaagca gctgaaacaa
    27661 gaagttgcaa agcttttagt tgagaacttt atgaaacagg tgtttgatga tggttttttc
    27721 catgctgatc ctcatccagg gaatctgcta ttccatgtac taacaaaaga agaacagaca
    27781 caagctagcc gaaaaactga aacagtccat gaaaaagagt tcggttcctt tgcttttcga
    27841 gcctccactt ctgcagaaga ccctgttgcg ccatatacca tcaactacat cgattttggt
    27901 atgatggggc atctttctgc aggacttagg caaaaactca cacaagccgt tttagcgctg
    27961 tatacgaaag atgcttatcg aatcgaaaaa gcggtccttc gactttgtca gcaagaaggc
    28021 tcgtttgatg aaagtcggtt ccaccaagaa ctgacaagtt ttcttgaaca gtattatgat
    28081 tctcctattg atgaaatcaa cttgcaagaa gtattcacac atgtcgtgac gatttgtcat
    28141 caaaataatc tgcagttcga tcgtgatatc acgcttttgc tgaaagcatt tgggacatta
    28201 gaaggggtga tccgcgtcct cgatcctgaa gtttccctca tggaagtcgc ttcgcctttt
    28261 gctcaacatt attttctcac tcatctggat gtagaagata cattgaaaca aagcggatta
    28321 gacttattgg aagggatgaa ggctgcgcct aagatccctc aacagctcca ccatctatta
    28381 gaaatgtgga cttcgggaca aggaaaagtc aatttggaat tgaaaaagca agacaaatta
    28441 ttgagcagga tcgaaagtat gatcaatcgg cttgttttcg gtatgatttt agctgcattg
    28501 atcgtagggt cctccctgct tgtccaagca gcacctgtag aaaacgcaga ggtcgtttcc
    28561 ttattaggga tatttacgta tgctattgcc gcattcgtca ttattttttt ggcaatcgat
    28621 gcattgatcc aaatgtataa gaagaggaaa aagtagaaaa aggttgtgac agaaagaatc
    28681 agcttcaaga aataagaagg ggttttacat aaattgtttc tcaaattttt gtgaaaattc
    28741 ggcttatttt tcgaagaggc tacttctgaa acaccgagaa taaaaaatgt gaaaaagctg
    28801 ttctgcaccc agtaataaga acaatcaaaa agggaacatg gaacacattt atcctgtttc
    28861 cctgttccct ttttgatgta ttcgagcata aataaaatac cttcgttacc tactatcttc
    28921 tgtacttggt aaaccttagt caattgcctt ttttaacgct tgacgaaaag acatatgatc
    28981 tttctccgta aagggttttg gaccctttgt ccttatgcca gcttttctta actttccact
    29041 atgataacgt tgttctagaa gcgtgccgat attcgcttcg tcaaattctt ttccagagtg
    29101 atggaacact cgcacaccct tcggcaatac aagtgaagct aaaccataat cttgcgtgat
    29161 caaaaagtca ttcttcttaa ccataccgac gattttataa tccgcactat cagctccgcg
    29221 gtctacgtag acgatttgga catgattagc gtattctttg gtcgtataat ggtccacact
    29281 ggtcacgata accacgtcaa gaccatagga ttctcctaat tttatgacat cttcttttac
    29341 tggagaacca tctccatcaa tgaatagcct cattcttttt cgccttcttc cataaatgat
    29401 aaacagttac taatccgctc agtaaaagac caacattgat ccattttgcc aagataagca
    29461 gttgtacact ttctatcggc actgattcat ggttcagcgt gatcaataat agatggattg
    29521 gctctcccat ttcttttgct aaaacaaaca aaagcgtcat caataaaaac tggtggatca
    29581 cactcccttt attttgagta gtatacttga caacgatccg gcgatccatt ggaatagctg
    29641 tatcagtttg ataatattcc ttatttagcc agctgcctgt tactacgtgc cagaaactaa
    29701 gcagaaagac acctatcgca aaaccatttg gaatcaagag gaccaataaa gaagaactca
    29761 gtccaagcaa ttgcatagcc aatggagacc atgttaaacg tttcaaaatc catggtccga
    29821 ctatttttgc tgcaatcaag cctgctgcat aaggcaaata cataaaaatc gaaagttttt
    29881 ctaatccgta cagtatgcca acataaaaac tgctaaataa aaaaaggaaa ttccctagca
    29941 ttccatttag aaaagtaaac agatttagcc agcttggcaa cttccattcc ttcttctggt
    30001 gtcctaaaac aaacacgaac aagatgaaca gcagcaagaa accataaata gcaagatcaa
    30061 aaagcctttc atcgaataac aatcgggcat ttctcaaaag aaacaataag ccaaagaata
    30121 tcaaaaataa aacaagctct cgcgtagcta ccagatttct tttgatgtct ttgaaatcca
    30181 gttcgtaacg cggataatga ctaaccgtat ggtaggccat gacgtaaaaa agtgtataaa
    30241 tagtaaagct tgctgcaatc tgagtcggtg caggcaactc aatcgcttga tacaggagaa
    30301 atagccagat agctgcaaat aagtactttt tcccatccat attgataaaa ccttgcgtct
    30361 tttcgtgaaa attgaccgtg gtattagcag gtacaagcca tgctgcactc agccctagca
    30421 aaacacttga aaaataaaat aaaggaaaat aaaatacgcc taatattcca agcaatgaac
    30481 cagcaccacc aatcagcaga gcgatcatca gcaacgtgta actatctaga ccaaatcgta
    30541 gactgttgac taaaaatagt ccagtcatcc gaaaggcata aaacagcgta aatggcaaaa
    30601 tcgtcagcca attatgcgag tctgttagtc ccaaaaataa aagataagga ataaaaataa
    30661 ccgtgttact taagataaag ggcgttgttt gtgtaaaatt acgcccaaat cgtttgagtt
    30721 tttcgaacat gacaatggct ccttaaacag tcttataact aatctgctct tcgattttag
    30781 cattaaaggg gaaataaacc aattttacag aatatattct tgtaaaaaaa aatacagaaa
    30841 gaagcataag cttctttctg catttttacg gtacttgatt actcaagcag acaagccctt
    30901 ctccacaaaa gtcttgcccg acacccctgc atccggcagg tgataaagat agaaatcaat
    30961 tatcctgtgc ctccaaaatc aattaggagc tctgtaaacc agagtccttc tcgacccgtc
    31021 gcataaattt actttaacac agttcctatg tttatgcgag agcttttttc attttataat
    31081 cgaccatttg aaatccttga ccgatatctg aaataagttc ttctgtaatc tcaaatcctt
    31141 catgcttata aacctcgatt gcttgatgat tcccctgatt cactcgcaaa aagatctctc
    31201 tttcctttgt ttgcgcagca agttcttcat accagcgaaa aactgaagaa gtcaatcctt
    31261 gaccacgtac tttatttagc aaatataatt tacttaaata aatttttcca tttatctctt
    31321 cataagcagt gtaaccgaca gtcttttctt cgctttttaa taaaaagtag gacacacctt
    31381 ctgtcagttc tttttgaatc tgtttttttg actgataagt tttcagcatg taatccactt
    31441 gtttttgacc aatgatcggc acaaaaactt ccggccatac ttcttgtatg actgccatca
    31501 gctcatccag ttcattttct gttactttta ttttttccat acacccacgt ccttttttac
    31561 attataaatg accttgcgct tgatggaata aaaagagcat gatcatgcta tacacaccca
    31621 ccgaccacgg tttcaagaaa atcacaatct gcataaattc cctaaaagat aatttcgtca
    31681 aaccagctac tagacagatc aagtcgtctg gcgcaaaggg aagcagcaag gtaacaacta
    31741 gtactttttt aatatctttc gtttttcttg tcagcaattc atcaaatttc tgatactttt
    31801 ttggcgataa aataagctgg acaaaggcta cgccatagta acggacaagc aaaaatccga
    31861 ttgcttcccc aatcactagg ccaatacaac tatataaaag cccaaatcca ttgccaaaca
    31921 tcagcatgcc cgctactgag gagatccctc ccggcaaaat aggaatgatc acttggatca
    31981 cttgcaacag aacaaatatc atcactgcat acttgccaaa ttgctggata aacgcttgca
    32041 aagacgtgct cgatcgaaaa atcccatgat gataagcata aagaatcaac acaaagaaaa
    32101 aaaacagacc aataatgggc atgatctgaa gacatttttg taaaactgat ccttttttag
    32161 acatctatat tccccacatc ttttttcatt tattaaaact gcctcct
//
LOCUS       DARBFO010000022        32178 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000022 DARBFO010000000
VERSION     DARBFO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.023"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(407..766)
                     /locus_tag="SCK51_001158"
     CDS             complement(407..766)
                     /locus_tag="SCK51_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEG1251798.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="SCK51_001159"
     CDS             complement(796..1137)
                     /locus_tag="SCK51_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251799.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="SCK51_001160"
     CDS             complement(1134..1808)
                     /locus_tag="SCK51_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG1251800.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="SCK51_001161"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="SCK51_001161"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEG1251801.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="SCK51_001162"
     CDS             complement(4322..5512)
                     /locus_tag="SCK51_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1251802.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="SCK51_001163"
     CDS             complement(5533..6060)
                     /locus_tag="SCK51_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEG1251803.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="SCK51_001164"
     CDS             complement(6107..8896)
                     /locus_tag="SCK51_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG1251804.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="SCK51_001165"
     CDS             complement(9043..9243)
                     /locus_tag="SCK51_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1251805.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="SCK51_001166"
     CDS             complement(9696..10016)
                     /locus_tag="SCK51_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251806.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10061..10204)
                     /locus_tag="SCK51_001167"
     CDS             complement(10061..10204)
                     /locus_tag="SCK51_001167"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251807.1"
                     /translation="MKFRLISEEATSGTPFKKEVVTWSRPLFDDYDTKNIIYEQNVNN
                     GRS"
     gene            complement(10519..11637)
                     /locus_tag="SCK51_001168"
     CDS             complement(10519..11637)
                     /locus_tag="SCK51_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251808.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="SCK51_001169"
     CDS             12108..13415
                     /locus_tag="SCK51_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEG1251809.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="SCK51_001170"
     CDS             13536..14735
                     /locus_tag="SCK51_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEG1251810.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="SCK51_001171"
     CDS             complement(14762..16030)
                     /locus_tag="SCK51_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEG1251811.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="SCK51_001172"
     CDS             complement(16200..16841)
                     /locus_tag="SCK51_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HEG1251812.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="SCK51_001173"
     CDS             complement(17250..17753)
                     /locus_tag="SCK51_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1251813.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="SCK51_001174"
     CDS             complement(17876..18640)
                     /locus_tag="SCK51_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEG1251814.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="SCK51_001175"
     CDS             18748..19023
                     /locus_tag="SCK51_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEG1251815.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="SCK51_001176"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="SCK51_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEG1251816.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="SCK51_001177"
     CDS             complement(20579..20980)
                     /locus_tag="SCK51_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG1251817.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="SCK51_001178"
     CDS             complement(20994..21803)
                     /locus_tag="SCK51_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1251818.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="SCK51_001179"
     CDS             complement(21790..22680)
                     /locus_tag="SCK51_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1251819.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="SCK51_001180"
                     /pseudo
     CDS             complement(22693..23172)
                     /locus_tag="SCK51_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23348..24289)
                     /locus_tag="SCK51_001181"
     CDS             complement(23348..24289)
                     /locus_tag="SCK51_001181"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HEG1251820.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="SCK51_001182"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="SCK51_001182"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG1251821.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="SCK51_001183"
     CDS             complement(25274..26470)
                     /locus_tag="SCK51_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1251822.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="SCK51_001184"
     CDS             complement(26488..27216)
                     /locus_tag="SCK51_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1251823.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="SCK51_001185"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="SCK51_001185"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG1251824.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="SCK51_001186"
     CDS             complement(28628..29086)
                     /locus_tag="SCK51_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1251825.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="SCK51_001187"
     CDS             complement(29083..30546)
                     /locus_tag="SCK51_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1251826.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="SCK51_001188"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="SCK51_001188"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEG1251827.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32178
                     /locus_tag="SCK51_001189"
     CDS             31602..>32178
                     /locus_tag="SCK51_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1251828.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         9385 a         6921 c         5511 g        10361 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataagatt tatgtcttta
    32161 tttttcaatt ttttaaat
//
LOCUS       DARBFO010000023        30690 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000023 DARBFO010000000
VERSION     DARBFO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.024"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..620)
                     /locus_tag="SCK51_001190"
     CDS             complement(<1..620)
                     /locus_tag="SCK51_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1251829.1"
                     /translation="MKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRTPI
                     REALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQKK
                     QVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERFRW
                     LRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDE"
     gene            943..2574
                     /locus_tag="SCK51_001191"
     CDS             943..2574
                     /locus_tag="SCK51_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEG1251830.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            2577..3656
                     /gene="uxuA"
                     /locus_tag="SCK51_001192"
     CDS             2577..3656
                     /gene="uxuA"
                     /locus_tag="SCK51_001192"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG1251831.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            3730..5157
                     /gene="uxaC"
                     /locus_tag="SCK51_001193"
     CDS             3730..5157
                     /gene="uxaC"
                     /locus_tag="SCK51_001193"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEG1251832.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            5241..5888
                     /locus_tag="SCK51_001194"
     CDS             5241..5888
                     /locus_tag="SCK51_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEG1251833.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            6017..7393
                     /locus_tag="SCK51_001195"
     CDS             6017..7393
                     /locus_tag="SCK51_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1251834.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            7404..8198
                     /locus_tag="SCK51_001196"
     CDS             7404..8198
                     /locus_tag="SCK51_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1251835.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            8211..9977
                     /locus_tag="SCK51_001197"
     CDS             8211..9977
                     /locus_tag="SCK51_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEG1251836.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            10005..11813
                     /gene="uidA"
                     /locus_tag="SCK51_001198"
     CDS             10005..11813
                     /gene="uidA"
                     /locus_tag="SCK51_001198"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEG1251837.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            11985..12386
                     /locus_tag="SCK51_001199"
     CDS             11985..12386
                     /locus_tag="SCK51_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1251838.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            12380..12625
                     /locus_tag="SCK51_001200"
     CDS             12380..12625
                     /locus_tag="SCK51_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1251839.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(12949..13170)
                     /locus_tag="SCK51_001201"
     CDS             complement(12949..13170)
                     /locus_tag="SCK51_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251840.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(13332..13404)
                     /locus_tag="SCK51_001202"
     tRNA            complement(13332..13404)
                     /locus_tag="SCK51_001202"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13369..13371),aa:Lys,seq:ctt)
     gene            13480..13710
                     /locus_tag="SCK51_001203"
     CDS             13480..13710
                     /locus_tag="SCK51_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1251841.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            13754..14923
                     /locus_tag="SCK51_001204"
     CDS             13754..14923
                     /locus_tag="SCK51_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1251842.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            15497..16066
                     /locus_tag="SCK51_001205"
     CDS             15497..16066
                     /locus_tag="SCK51_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEG1251843.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            16053..16913
                     /locus_tag="SCK51_001206"
     CDS             16053..16913
                     /locus_tag="SCK51_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1251844.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            17103..17807
                     /locus_tag="SCK51_001207"
     CDS             17103..17807
                     /locus_tag="SCK51_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1251845.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            17812..19647
                     /gene="walK"
                     /locus_tag="SCK51_001208"
     CDS             17812..19647
                     /gene="walK"
                     /locus_tag="SCK51_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEG1251846.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            19644..20960
                     /locus_tag="SCK51_001209"
     CDS             19644..20960
                     /locus_tag="SCK51_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251847.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            20961..21830
                     /locus_tag="SCK51_001210"
     CDS             20961..21830
                     /locus_tag="SCK51_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEG1251848.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            21885..22694
                     /locus_tag="SCK51_001211"
     CDS             21885..22694
                     /locus_tag="SCK51_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG1251849.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(22797..23426)
                     /locus_tag="SCK51_001212"
     CDS             complement(22797..23426)
                     /locus_tag="SCK51_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEG1251850.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            23596..24888
                     /locus_tag="SCK51_001213"
     CDS             23596..24888
                     /locus_tag="SCK51_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1251851.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(24964..25626)
                     /locus_tag="SCK51_001214"
     CDS             complement(24964..25626)
                     /locus_tag="SCK51_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG1251852.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            25851..26135
                     /gene="groES"
                     /locus_tag="SCK51_001215"
     CDS             25851..26135
                     /gene="groES"
                     /locus_tag="SCK51_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEG1251853.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            26186..27811
                     /gene="groL"
                     /locus_tag="SCK51_001216"
     CDS             26186..27811
                     /gene="groL"
                     /locus_tag="SCK51_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEG1251854.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(27900..29048)
                     /locus_tag="SCK51_001217"
     CDS             complement(27900..29048)
                     /locus_tag="SCK51_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1251855.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(29150..29734)
                     /locus_tag="SCK51_001218"
     CDS             complement(29150..29734)
                     /locus_tag="SCK51_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEG1251856.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(29861..30292)
                     /locus_tag="SCK51_001219"
     CDS             complement(29861..30292)
                     /locus_tag="SCK51_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEG1251857.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(30310..30630)
                     /locus_tag="SCK51_001220"
     CDS             complement(30310..30630)
                     /locus_tag="SCK51_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1251858.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT        10463 a         4691 c         6111 g         9425 t
ORIGIN      
        1 tgttcatcaa gcattaaatg aagatgcaag gatgttaaga agttagcttc ttcagggtct
       61 tgcatggtca aggctttaaa tagttgatga tgttgttcca taattttttc ccattttaat
      121 ccttccgttt ttactcgtaa ccaacgaaat cgttccaagt gcgtattatt atcttccaac
      181 cattgccata cttcttcccg ttctgcaatt tcaaaacaag ctttatgaaa taaattatca
      241 ttatgaaaaa aactacgagc attcttttga ctaacagctt tttcttgttc tgcaattata
      301 gtttctaaaa cttgtttctt ttgattattc atcttggcac aacactctag cataatttgt
      361 cgttctaaat gttctctgac aaaacgagca ttttttgcta aatctaagtc gattaatgag
      421 atataatttc cactttgagg aattgtatca acaagtcctt gatgacggag ttgaatcaat
      481 gcttcacgta ttggagtacg accgatagaa agcgtttctt ccaatttttt ttctgatact
      541 ttttttccag gttctaagtc agcgtagata atttgctgac gaatagattg ataagcttga
      601 ttttggagac tagttttaat tgtcatattt aatccctctc aaaatttcat ttttgaattt
      661 gatattttag ttctatgctc gttatataat atcaaatttc aaagtaacat actagctatt
      721 aaaaaaacaa aaaatactca aataagatca aagagaaatt aagctttaat ttaagttagt
      781 tctaaaaaat atatattggt gtgcttttac taaaaaaaga cacaataatt ctgacatggt
      841 agaaaaaaga atatttttag ttttttctta ttgacagcga ttacattcga ttttatacta
      901 caattgtaac ggaaactaac atatcagtat ggaggggtaa atatggtttt gattaaagac
      961 tggcaagatt ctatcgaagg attgaacaaa atgcaagttc aactaccgta ttttaatgtc
     1021 gaagcggtca aacaagaagg tcagaaacat ccagtgtggc ttcattttgg tggaggaaat
     1081 ctttatcgag gatttcatgc agaaattgct caatgcttag cagatcaaaa tgaattagaa
     1141 tccggagtag ttgtgtgtga gacatttgat gaagaagtag tagaaaaagc ttatcatgac
     1201 tacaacaatg atattttgga gattgttatg ggtgaagatg gtgaactcaa aaaaagactt
     1261 ttagcagtaa cagctgatag tttatattgc aatccaaaga atataacgga ctatcaaaga
     1321 gtggttaaat attttgaaga tcctacatta cagtttacaa cgtttactat tactgaaaaa
     1381 ggatacagtt taaaagattc taaaggacaa tttttttcgg tagtggaagc agatataaaa
     1441 tctggaccta ccaatcctca acatacgatg agtattgtga catccttgtt gtatagcagg
     1501 tttaatgctg gagagttacc gattgcgatg gtttctactg ataatttctc acagaatggt
     1561 caacgttttc aagatagcat ttttatgatt gcagaagctt ggcaaaaaaa aggatacgtt
     1621 tctgaaaaat ttattgctta tttaaaggat aaagaaaaag tgacttttcc ttggagtatg
     1681 attgatagaa ttacgccaaa tccttcggaa agtgtcaaaa tggatttatt gaaggcgggt
     1741 ttcgaagact tagatataat tcatactgcg aaaaatacca atatcgctgc ttttaccaat
     1801 actgaaaaag tccattatct agtcattgaa gatagttttc caaatggtcg acctaaactg
     1861 gaagcggctg gtgtgattat gacagataga gaaacagttg ataaagcaga tatcatgaag
     1921 gttacgacat gtttaaatcc gctccatacg gcattagcaa taacaggttg tttgttgggg
     1981 tataaatcaa ttcaggcaga aatgcaagat gatgatttgg ttggcttaat taaagggata
     2041 ggttatgtag aaggcttacc agtagttgaa agtccaggcg ttattgatcc aaaacaattt
     2101 attgatgaag taatcaataa acgtttgcct aatccctata ttccagatac accgcaacga
     2161 attgcaacag atacttctca aaaagtagcg attcgctatg gtgaaacaat taaaaagtat
     2221 gtcgaaaaag acggaaatgc agaaaatcta acatttattc ctatcgctat tgctgcttgg
     2281 ttacgttatt tattggcagt cgatgatcaa ggaaaaaatt ttgtgccaag tcctgatcct
     2341 ttattggtag agttgcagga aaaattctca gtattgaaat taggtgaaca agatcctgca
     2401 aagattcatt gtgcggtaca ggaaatctta gaaaataagg aaatatttgg tttagattta
     2461 tatcaagttg agataggaaa gaaaattgaa aaaatctttt gcgaaatgct agtaggtcct
     2521 caagcagttc gtaaaacaat caataaatat ataacagaag ttggaggaaa gtaactatgg
     2581 aaatgacttt tagatggtat ggggcaaatg aagaaaaaat ctcactacaa gaaattcgtc
     2641 agatcccagc aatgaaagga attgttggaa cattaatgga tataccagcc ggagaggtct
     2701 ggccgagaga acgaattaaa gctttaaaag atgaagtcga agccaatggc cttaccttga
     2761 aagttattga gagtgttaat gttcatgatg atatcaaaat tggtttgcca acacgagatc
     2821 aatacatcga gaattataaa cagacgattc gcaatttggc agaatttggg attgaggtta
     2881 tctgttataa ttttatgcca gtctttgatt gggtaaaatc agatttggat tatcatttag
     2941 atgatgggtc ttctacgtta gcctatattt caaaaaatat tcctactgat ccagaggaaa
     3001 ttattagaaa agtagaaaat tctactaata attttgaatt gccgggttgg gaacctcatc
     3061 ggttatcaca aatcaagcaa ttattagaag cctataaaga agttgatgaa gcgaaattgc
     3121 gagagaactt tgcatatttt ctcaaagcaa ttatcccgac atgtgaagaa gttgggatta
     3181 aaatggcagt tcatccagat gatccaccat attctatttt cggattacct cgtattgtta
     3241 aaaatcgtga cgatttagaa tggttgtgta atgtagtgga ttctccttct aatggtatta
     3301 cgttatgtac tggaagtatt gcagaacgac cagataatga tgtctatgaa attctatctg
     3361 aattttgtca acgggatcgg attccttttg cacatgttcg taatatcaaa tatttaagtg
     3421 ataatggact cgatttttat gaagcgccgc ataaatcaga atatggttct ctagatatgt
     3481 ataagatatt aaaaacaatg tacgataatg gatttaatgg ctatattcgt ccagatcatg
     3541 gtcggatgat ttggggagaa accggtagac ctggttatgg gttatatgac cgagctctag
     3601 gtgcagttta tttaaatggt atttgggaag cgcttgaaaa gggggcgagt aaataattat
     3661 ataatggagt ctatttagaa gaaaaatttt ctcttctaaa aactaaggga aggaaaggag
     3721 tgcttaaata tgtttttaga taagaacttt ttattagaga atgaaatagc taaaagactt
     3781 tttcataaat ttgcagaaca ccaaccaatc atcgattatc attgtcattt agacccagca
     3841 tgtttgctag aaaataagca atacaagaat ttgactcagg tatggttaaa tgataaagga
     3901 gctggagatc attataaatg gcgattgatg cgagctaatg ggatagaaga agagttgatc
     3961 agtggtgcag gtaatgatta tgaaaaattt ttagcttttg taaaaacagt ggagaaggcg
     4021 ccaggtaatc ctatttttga atggtctcat ttggaattac aacgttactt tgatattgat
     4081 ttgacaattt ctgagaagaa tgcaccagaa atttggcgac gtgccaacga aaaaatccaa
     4141 acggattcct atcgcccaaa agaattgctg aagatgatgg atgtgaaagc catttgtaca
     4201 acggatgatc ctacagatga cttgcgatat catactgagc tttctaaaca ggataattct
     4261 ttcaaggtat tacctacttt ccgtccagat aaggtctgga gtatcaaatc atcaggattt
     4321 tcgaactata ttttaaaact tagtcaagca gcgggtatac caatcaaaaa ctttaatggg
     4381 ttgaagcagg cattgagcca acgagtggat tattttcatc aaattggctg tcgattagca
     4441 gatcacggtt tgaatactta tttttatgaa atagcagatg aaaaaatact cgacaatctg
     4501 ttaataaaag ctttagcagg aaaaacggat ttccatttat cagaaattgc acaatttgtt
     4561 acaggtattc aattacattt gatgtctgag tatacaagat atggttggac gatgcaaatg
     4621 catttaaatg ctttaagaaa taattcaagt atattaaagg aacaaattgg agtagatgtc
     4681 ggtggtgatt cgatggggga tcaagctagt ttgacacaaa atgttgtagc attgttcgac
     4741 actgctgaaa agagtgggtg cttaccaaaa acaattttat attcattaaa tccaaccgat
     4801 tggcttccac tagcaacagc aatgcaaagc ttccaagggg gtatgattca aaaaatccaa
     4861 ttgggatgtg cttggtggtt caatgataca gctgaaggaa tgcgtcaaca gttgactacg
     4921 atggcgcaac aaagtttgtt gtcgaatttt acaggtatgt tgactgattc tcgaagtttt
     4981 ctaagctatc ctcgccatga gtatttccgt cgagtattat gtcaattact aagcgagtgg
     5041 gttaaagcag gtcgaattcc agatgatgaa atgtatctta gtcagatagt ggagaatatt
     5101 tgttatcgta atgcgtataa ttattttaat ttttttgaaa tcgaaagtag aaaataatac
     5161 aaaaaataaa agcttaaatt tatttattgt aagttagtac aactagctag agtatgtatc
     5221 cataaaagaa ggagatagat atgaaaaaag tagaaatttt aagtaagtta gaagcggctg
     5281 gagtcattgc ggttgtacgt ggaaaaaata aagatgaggc cattaaagca ataaatgcaa
     5341 ttactgcggg tggaattaaa gggattgaac taacttttac agttccgaat gctgaagaaa
     5401 taatcaaaga aattgctaaa aaatatgctg atcaaaaaga tgtaataatt ggagccggta
     5461 cagttttaga tgcgtttact gctcgtattg caattatggc tggagcagaa tatatcgtaa
     5521 gtccatcttt tgatcgacaa acagcagaga tttgtaattt atatcaaatt ccttatttac
     5581 caggttgtat gacaattact gagataaagg aggcacttac cgctggagta gaaatcatta
     5641 aactatttcc aggttctgct tttggtccat ctgttgtaaa aacgtatcaa gctccattac
     5701 catatgtaaa tataatgcct actggcggag taagtctaga taacttagaa gagtggtttg
     5761 aagcaggcgt tgttgcagtt ggtgtaggtg ggaatttgtt ggctcctgtt gaaaaaggtg
     5821 attttgaaga agtaactcga atagctagta aatatataga taagttttat gagataaaga
     5881 attattagga gaaaaaataa ataatactat aaaatgtaaa aaataaatac taactcttta
     5941 tacttcagag tatagtacgt aagttggcag ttcaacagaa tgaaaacgct taacaatagg
     6001 aaggagcgca aattatatgg aaagaaaatt tggtctaaga gacaaaatag gatatatgtt
     6061 tggagatttt gggaatgatt tcactttcat ttttgctagt tcttttttaa tgatttttta
     6121 tacaaaagta ttggggataa gcggagctac cgtaggaaca ttatttttag tagcaagatt
     6181 tattgatgca gttacagaca taacaatggg gcgtattgtg gatggatcaa aaccatctaa
     6241 aactggcaaa tttaaaccgt ggattttacg gatgagtggt ccagtcgctt tagcaagttt
     6301 tttaatgtat caaacatcaa tggcatcagc ttctatgagt attaaagtta tatatatgta
     6361 tgtaacatat atactatggg gaagtatttt ttatacatct attaatattc cttacggttc
     6421 aatggcatca gctatcactt ctgttgcaga agaaaggaca gcgttatcaa catttcgtag
     6481 tataggagct accttagcag gtatgttcat tggcgtgtta acaccaattt ttatttatac
     6541 aagagatagt tctggagctc aaattgtacg tggtggatca acttttacaa ttgtggcagg
     6601 agtattttca gtgtgtgcag ttatttgtta cgtaatatgt tataaattaa ctgtagaacg
     6661 ggttaaaagt gatgaatcgc agttaaaagt ttcattgttg aaatctttaa aaggattagc
     6721 taaaaataga gcgttgcttt ctataatagc tgcagctatt tttttgttgc ttgctcagct
     6781 tttaacaatg agtatgaaca attatgtatt tccagattat tataataatg cacagggaat
     6841 agttttaatg aactttatca atcctatttt gatgttggta cttgtttcac ctgtaagttt
     6901 aattttcagt aaaaaatacg gtaaaaaaga attggcgtca gtagggatgt tcttctctgc
     6961 tattgtatat ttacttttat ttttgataaa gcctagtaat atgtatgtat tcttagttct
     7021 tacttctgtt ggatatatgg ggttaggtat ttttaataca gtcatttggg cgaatattac
     7081 tgatgtaatc gatgatcaag aagtgaaatc tggccaaaga gaagatggaa ctatttatgc
     7141 agtatattct tttgcccgaa aaattggaca agctttagct ggcggtgtcg gaggttggac
     7201 tctttctata atagggtatg attcgttagc aaaagtacaa agtggagaag ttttacaaaa
     7261 gctgtataac tcttcaacgt tgattccagc agtttgtttc tttgttgtag gattgatttt
     7321 atttttcgta tatccattat ctaaagaaaa agtattagaa aattataatt tgctaaatca
     7381 aaaacataac taggagaaaa aacatgaaca gttggatatt ttgtattgat tcagatggtt
     7441 gtgtcatgga tacaatgact tataaacaca atctcttttt cgggccaatt gctgcagaga
     7501 tttttgatgt tcatcatagg gaaaaattcc tacaggattg gaataatatt aatttatatt
     7561 cagagactcg tggaataaac agatttttag gtttagttaa aacattagaa aaaaatgatg
     7621 taccatcgat taatcgatta aagcactggg tatctgtaac aaaagaattg tctaatccat
     7681 ctttgaagag ggaaatagaa tactctcagt cagaggattt attaaaagct ttaaaatggt
     7741 cggaagaagt caatcgacag attagtcaag ctaaaggatt agataaaccc tttttaagtg
     7801 ctagagatac tataaaagct ctgaaaaaat atgggaagat aattattgta agctcagcta
     7861 ataaggaagc tattcaagaa gaatgggaaa gacatgaatt attatcctta gttgatgaac
     7921 tgtgttgtca agataaagga aaaaaagaag acattattcg aagtgtaatt gaaaatggtt
     7981 gtgatttgga taagatatta atgattggtg attctcctgg tgatctagaa gctgcaaata
     8041 agaataaagt tttcttttat cctattttgg ttaataaaga aaaagaatca tgggaaaact
     8101 taagaactga tattttattt aaatgtttat cgggagagta tatagatatt gaacaaaaat
     8161 atatagatac tttttggaaa aatcttacta ataagtagag gagaaagaaa atgactataa
     8221 aagtagactt acgaaaaaaa ccatatcatt tagatgaaga tgctatttct tggattgaag
     8281 agacgattga tgctatgact ttagaagaaa aaataggtca attatttgtt aatatgggat
     8341 ctagtcgtga taaaaattac ttaagtagta tggtcaatca gtaccatatt ggagctgtac
     8401 gatataatcc aggaactgct gatgaggtat atgatcaaaa ctatatccta caaacagaaa
     8461 gcaaaattcc tttattaatt gctgctaata cagaagcagg tggtaacgga gcttgtacag
     8521 atggtaccga agttggtgta gaagtaaaaa ttggtgcaac aaatgatgcg aaatatgctt
     8581 atgaattagg gagagtttct ggaattgaag catcggctat tggatgtaac tggagttttg
     8641 ctcctattgt agatattaat agaaattgga gaaatcctat catttctagt cgatcttttg
     8701 gacaaagccc tgataaagtt ttagaactta gtttagctta tatgaaaggt attcaagaga
     8761 gtggaatttt gcctgcagct aaacatttcc ctggtgacgg tattgacgaa cgagatcaac
     8821 atctatcttt tagtattaat agtttaagta cagaagaatg ggataattct tttggaaaag
     8881 tatactcagg cttaatagaa gctggattac cttcaattat ggcaggtcat atccatatgc
     8941 cagcttattc aaaacatttt aatcttgatc tgaaagatga agatttactt cctgcaactt
     9001 tatcaaaaga attaatcact gacttactta gaggaaaact cgggtttaat ggattagtag
     9061 ttactgatgc tagtcatatg ttaggaatga ctggggcaat gaaacggtca gaattattgc
     9121 ccacttctat tgcggctgga tgtgatctat tcctattttt taatgatcca gatgaagact
     9181 ttaattatat gatggaaggt tattacaaag gaattatttc agaagagcgt ttacgtgatg
     9241 cattaagaag aattttaggc ttaaaggcta aactaggttt gcataaaaaa gaaaagaata
     9301 aaattttgtt accgaaacaa gaagctatgg aaaaaattgg tttaattgaa aataaatcaa
     9361 tttttaaaga ggtagctgat caagcaatta cactagttaa agataaacaa gatatttggc
     9421 ctattgtacc agaaaaatat aaaaggattt tactggtaga tgtacatggg gtaaaaggtg
     9481 ggtttggtga agtagttgct ggaaatagac ctcaggccat tgatttattg aaacagattt
     9541 tgtcagcaaa aggatttgaa gttgaaaaat acatttcacc tgaagaagaa ttgaaaggat
     9601 tatcgcaaga agaaaaaatt aaaaatatag ctggaatata tgctgctaag agaccaatca
     9661 aagatttaac tgaaaaatat gacttgatta ttaatatagc taatgttaac ccaggtacag
     9721 ttcaaagaat tgtttggcca gcaagtaaag gtactccgga tattccattt tatattcatg
     9781 aaattccaac aatttttgta tctgtacagt gtccgtttca tttagctgat gtgcctcaag
     9841 taaaaactta tatcaatact tatgatggaa aacaagaaac tttagaaata ttagtggaca
     9901 aactaatggg cctttctgaa tttaagggaa taagtccaat agatgcttat tgtgggttaa
     9961 aggatacatt tatttaatat ttaaattata taggagtgat ttacatgtta tatcccatta
    10021 tgacagagac gaggcaagta attgatttaa gcggtctttg gagatttaag ttagatacaa
    10081 atgatgactt acaagaagaa cttgctttag aaccaatggt tgaaaaagag agttatccaa
    10141 tttcagttcc ttcttcttat aatgatttat ttgaaacaga gaacattaga aatcatgttg
    10201 gatgggtgtg gtatgaaaaa gaagtgattc ttcctaaaaa attattagaa gaaagattag
    10261 ttcttcgttt tggatctgca acacatgaag caaaagtatt tttgaatgga aaattgatta
    10321 atcaacacaa aggagggttt acgccatttg aatcagaaat taatcagttt ataaaagatg
    10381 gaagtaacag aattacagtg gctgtaaaca atgttattga cgatagcact ttacctgtag
    10441 ggattttgaa agaaacgata caggaagatg gagacataaa aaaagaaaat ttggttaatt
    10501 ttgacttttt caattatgca ggcattaata gacccgttaa attgtataca actccgaaag
    10561 actatataaa ggatgtcact attaatacaa aaataattga ttcagatgca aatgtagaat
    10621 ttaatatcga tacagaatgg accggagaaa ttctaatagc aatctatgac gaagaagctc
    10681 aacttatagc agaagctaag gggaaagaag gagtgattca acttaagaac ccaacacttt
    10741 gggagcctct aaatgcttac ttatatagta tgaaagttag tttaattaac gaaaataaag
    10801 tgattgatac gtacacagaa gaatttggca ttcgtagtat tcaggtttcg aatgggaagt
    10861 ttttgattaa taataaacct ttttacttta agggatttgg gaagcatgag gacacgtata
    10921 ttaatggtcg tggtttgaat gaagcagtaa atatcaaaga ttttaattta atgaaatgga
    10981 tcggtgctaa ttcatttagg acttcacatt atccttattc agaagaaatt atgcgcttag
    11041 cagatcgaga aggtattgta gttattgatg aagtacctgc agtaggatta catttgaatt
    11101 tcatggctac tttattagat gacaacgtag aaaaacatga tacttggaaa gaaatacgga
    11161 cagaaaatgc tcacaaaatg gcgattaagg aattgataga acgcgacaag aaccatccga
    11221 gtgtcgtaat gtggtctatt gcaaatgaac cagattcgga ttctgaaggc gctaaagaat
    11281 attttgaacc tttagttgaa ttgactaaag aacttgaccc tcagaagaga cctgtaacaa
    11341 tcgttactta cttaaagtca acaccagata tttgtaaagt aggagatatt gtcgatgttt
    11401 tatgtttaaa tcgctattat ggttggtatg tttcaggtgg agacctaaaa catgcaaaat
    11461 atctgttgaa aaaagagcta gatggatgga tggaaagatg tcccgatacg ccaataatgt
    11521 ttacagaata tggagcagat acagtagctg gacttcatga tacagtacca acaatgttta
    11581 cagaagagta tcaagtgggt tattacgaag caaatcacga agtaatagat aaatgtgaca
    11641 attttgttgg tgaacaagtg tggaattttg cagattttga aacaagtcaa gggattttaa
    11701 gagtgcaagg aaataagaaa ggaatcttta ctagagatag aaagcctaaa atgattgctc
    11761 actatctaaa gaaacgatgg tcatcaatac caaatttcag ttataaaaaa taatttacct
    11821 tttataagga atataaaaaa ggagtggctg cttaattgcc aactcctttt ttttataaaa
    11881 agattttctg tttaaagaag gatatttttt tctagttttt tcgaaaaaaa ggcgctttct
    11941 gattgttgta ttagtggaaa ggggaaaaaa ggagcatatt tatcatgcaa caaaattaca
    12001 atgagaaatc tatacaacat caatttgatg cctactgtaa aaaagtatta cgaaacgaaa
    12061 ctaagtctat tcgaaaacga aacattaaca taacagataa tgaggaacca ttgacgaatt
    12121 caaatgaagg taagtactcc caaactcatt ttgatgaaca agatgtctat ttcttatttg
    12181 gaatggaaat attaatttca gatcaaaagt tatcggcagc cattgatcat ttatcggaaa
    12241 taagaagaaa ggttatccta ctttattatt ttgcaggttt taatgatact gaaatcggta
    12301 aaatcctcaa tatgagtaca agtggtatct ggtatcaacg aaagaaggca gttgaacaat
    12361 taaaaatggg gtatgactta tggtagacaa ccccaaaaaa cggcagtatc cattagttcc
    12421 tctatccatt atccaatctg ctctagaaga agatccaatt tcaatcaaga tgatactcaa
    12481 acattatcat agttatatag ctaagttatg tttgacaaat ggatttaatg aggcggggca
    12541 atttattacc tatgtagatg agtatatgct aagacagcta gaaattaaat tgatagaagc
    12601 gatcttaaaa tttaaaatta actaactaaa aagtcccctt tccacttatt ctgtttaaaa
    12661 gaatcttcta atagttaaag gattagtaga ttcttttaaa cagaaaacct attgatcatt
    12721 gaaatttaaa tacattctat cagtacgtta acacttcaag gagcatattt tagttgcgcc
    12781 atgatttctt actgtaaaga aggagcgata aacattctaa aataattttt atagtcaata
    12841 aaaagcgatg atttgaagtt gggataatga tacttccgta aggctcgccg taaacttgga
    12901 gagaggtgaa attcctatgt gcttttgaca aaagcagctg attaagaatt atttccatat
    12961 gttgatttca acattttttc tattattttt aagaccaaat tcagtgaggc aaatagcatc
    13021 atgtaaattt agtttttcta ttgaacgttc tccacttttt agttttttta tagtactcaa
    13081 atttaatcct gtatgtttag agatctcatt tgcagtatgg ttttgtaaag tttctcggat
    13141 tattgttata ttagcaattt cattacgcat aattatgttc ctttcttatc aaaaatatca
    13201 atgactacag tgggctttta gtgaaaaaaa ggggtactac agtctctttt gaaaacaaaa
    13261 aaatacagta tatacgtttt ctaaatgcta agcaccaacg tttacactgt atttaagggt
    13321 ttggcaccca atgggctgtg aggggctcga acccgcgacc cgatgattaa gagtcatctg
    13381 ctctaccaac tgagctaaca gcccaaatta atactttgtt attctaacac ctaaaataaa
    13441 aaagtgcaat acttattatc agaattgaga gtgaatttta tggattttca agattatgaa
    13501 aaaatatttg aaagttatcc atcgatattg aaaattactg atttaactaa aatgttaaat
    13561 attggaaaaa ataaagctta taatttgctg aaagatagat caatagaaaa tataaaaatt
    13621 ggagcaaggt acaaaatacc taaaaaagct gtcattgagt atatattgaa aatacaagat
    13681 gaaggtaaaa atcatgctat aataaaatag tcaaaatttt tgccgatatt tcagtttaag
    13741 aggagagatt gaattgaaat taagagcaaa cataattgaa aaaagaggaa cattacaagt
    13801 agttgtatct tataaagact ctaacggaaa aattagacaa aagtggaaag atacaaaatt
    13861 aaaaaagaaa ggaaataaaa aacgagcaga aaaaattaga gatgaatttt tactagaatt
    13921 ggaaagtcaa ttagctatgg aatgtgaatg tgctaatcca aatttattgt tttatgacta
    13981 tttgcttaat tatttggaag tatcaaaaaa acaagtagca tttaacacat atgttgggta
    14041 taagcattac gtccataatc gaatttacaa gtttttttat cctaaaaaaa ttaaactaaa
    14101 taggttaaag ccttatcatt tacaagagtt ttatcaatat atgttaaatg ataactgtac
    14161 agcaaatacg gttattcatt atcacgcatt tatacgcaaa gctcttcaag aggctgttat
    14221 tacagaattg ataagtacaa atgtagcaga taaagtaaga aagccaaaaa agaaacaatt
    14281 tgtttctcaa gtttataatc aacaagagat cttgaaatta cttgacattt tgtcagaaga
    14341 aaaactctat ttggtagttc ttttaacagc attttatgga ttgcgaagaa gtgaagtttt
    14401 aggattaaaa tggtctgcta ttgatttttt aaacaaaaaa atcatgataa accatgtaat
    14461 catagaaaat ccagagaata tgtcacagtt aataaagaaa gatcaaacaa agaactcttc
    14521 cagctatcgt acattacctt tggttgaaac gattgaacga gcattagtaa atcaagctaa
    14581 atggcaacaa gataatcgta ttcttttagg agatgattat attttagaag ataatgagta
    14641 cgtatttact atggaagatg gacggttgat gaagcctcaa tatgtaacgc atcgtttgag
    14701 taagttgatt aagaaaaatg gattgaaaaa aattcgcttt catgacttgc gtcattctaa
    14761 tgcttcaata atgttagata gtggccagaa tatgaaatcc attcaagaat ggctgggaca
    14821 tgcatcgtat tcgacaactg ctaatttata cacgcattta accgcaggag taaaagatcg
    14881 tgcagcagaa gcattagaaa acgcatttgg gtttaaagaa taaaaaaaca aaaaacaata
    14941 tctttggggt atcgcttctt gtttttagaa tttttttgtt ggcaatttgt tggcagtatg
    15001 ttggcaatga aaaaattcac aaactaaaaa cctatttata aagctttata aaactttttt
    15061 cacaaataga gcatttggtt aagagtcagc tgctctacca actgagctaa cggcccgtac
    15121 aaaaagtact taataatagt agcactaaga attttaaaat gcaagactac atttaaagaa
    15181 aatcagaaaa atctttactt ccttatagga aatcattcat agattattca catttgctcg
    15241 ctattatata agcataccaa caaacaaagt tcttgcaaga gaacaaaccc taacactttt
    15301 ttcatttttt actcctttcc cgccgtctcc ccggcgggtc tcctgttctt aacgataaaa
    15361 gacaagaagg cttagccttc ttgttttttg ttttcatgga atcccgctaa tcaaatcacc
    15421 tagttaaagc aactagattg aatgtttatc ataagtctga tatcatgttt atgaaatgaa
    15481 aaaaggagcg gttatgatgg aaaaaatgcg tcagcaatta atggagtggg ggaaggaact
    15541 agaaacattc cgtctgcctc actgggaaga actccctgac ttagaacttt atatggacca
    15601 agtcagaacg ttagttgatc gttatctttc acctgtgata caaggagaaa agcatccatt
    15661 gcttacctct tctatggtaa acaattacgt taaactaggt ttgataccag caccagttaa
    15721 aaaaagatat aacaaagaac acgtagcatt tttacttgca atcactacgt tgaaacaagt
    15781 attgactatt cctgaaatca aagaagggat attgttccaa ggaaaaacag taggaattcg
    15841 tgaagcctat aatctatttt gcgatgaaca agaagcagct gtgtggatgg tcagccaatt
    15901 agcacaaggt aaatctcatc cgcaaaaatt cgaccaggca acccctgtag aatatattgc
    15961 tgtaagagcg gcaactttat cgtttgcaat gaaacttctt gcggaaaaaa cgattgttct
    16021 agaaaccgaa tatttaaaag aggagaaaac aaatgaaaaa caataaaatc gcaattttag
    16081 tagattcagg tacggatgtt ccagaagaat tgataaaaaa atacgagatg tatatgatcc
    16141 cattgaaaat tatttataaa gatcaggtat atacagataa aattgacatt acccctgaag
    16201 agatctatca acgtttgcca gaggaaatcc ctggtacatc tttgccggat ggtgagtcga
    16261 tcacagaaat ctttgagcaa atcaaaaaag acggttatga aaaggtactt gcggtaacaa
    16321 tttccagtgg attgagcggg acatataatg tggttcgttt aattgcacaa cagcaagaag
    16381 aattggaagc atatgtacta gatacgaaaa atattggtat tggtgcaggt ttttctgcga
    16441 tccaagcagc aaaatggctg gaagaaggag tagaatggga tcaactgatc agtaatctga
    16501 atgagttagt cgaaagaaca aaagttttct ttaatgttgc gacattggaa tatcttcaaa
    16561 aaggtggacg gatcggttta gttgcttcta ttgtaggaac cgctttaaag cttaacccaa
    16621 tcatctcatg taatgaagaa gggatttatt acacagtagg aaaagctagg ggacgtaaaa
    16681 aaagtttgga taaaacggtt gcttacgtca aagaaaaaat cggtaatgca aaggtcttca
    16741 acttagctgt tgcccatggg gatgcgaaag aagaagcaat tgaaatggaa gcgagactta
    16801 aaagagagtt cccacaagca aaccaaatct actttggcca gatttctccg gcattagtag
    16861 tccacactgg tccgggatta ttgggtgtcg gtgtacaagt tttagaagga taaatcaaaa
    16921 aaaggaaaca ctgaggtgaa aatcagtgtt ttcctttttt attgaagtta gaacaaaaaa
    16981 taataaagta ttttgcactt tcattttaat tttctctttt gttttatagg caaagatgaa
    17041 acaaaagcag aaatacggta taatgagaag gtatgacaat aatatataag aggtggctct
    17101 ttatgaaaaa agttttagtt gtcgatgatg agaagccgat ttcggatatc gtcaaattca
    17161 atttagccaa agaaggctat gacgtttata ctgcatatga tggggaagaa gccttggaaa
    17221 aagttgctga ggtagaacct gatttgattt tacttgattt gatgctacct aaaatggacg
    17281 gcttagaagt tgctcgtgag gtacgcaaaa cttatgatat gccgatcatt atggtaacag
    17341 ccaaagattc tgaaattgat aaagtcctag gcttagagtt aggcgccgat gattacgtga
    17401 ccaagccatt ttccaaccga gaattagttg cacgtgtcaa agcaaatctt cgtcgcggtg
    17461 ccacggctgc gaaggaacca gaagaggcag cacctgcgga attgacgatt ggtgatttga
    17521 cgatccatcc agaagcttac atggtgacga aacgaggaga aacaatcgag ttaacacatc
    17581 gtgaattcga actactcttt tacttggcga agcaccttgg acaagtgatg acaagagaac
    17641 atttattaca aacggtttgg ggttatgatt attttggaga tgtacgaaca gtggatgtaa
    17701 ctgtacgtcg tttgcgtgaa aagatcgaag ataatccaag ccatccaaat tatttagtga
    17761 cacgtcgtgg tgtcggttac tatttgcgta acccagaaca ggagtaattg gatgaaaaga
    17821 aaagtccgct tttttcgatc agtcaatttt aagatcgcta ttacctttat tctgatttta
    17881 ttgatctcga tcgaaattat tggtgcctac tttatacgtg gtttagaacg ttccacgatc
    17941 aatacattta ttaaagatat gaatcaaacg gtggaatcat tagcaacaac aatcagcccg
    18001 gaattgaatc gaaaagataa tgcagatgat gaagaggtaa atgcgaatat caaaagattt
    18061 attgaaaaca gtgcgactag cgatatcatc gaaatacggg tcgttgacga aaaagggatc
    18121 atccgaggaa caaccgatgt caatgaacaa agcgctgtgg gaaagaaaaa tgattatgtt
    18181 gatatcaatg atttcacgac taaacgttat gttgctttag ataacgacga taaacgggtg
    18241 aatatcaatg ttcaaccgat cctgtccccg accggcgatg ccgttatcgg ggctctttac
    18301 gtcaaaagta atatcgaaca aaaatattcg gaaatcaata acacagcagt cattttcttt
    18361 actgcctcac taattgcggc atttatttca atgatcgtat ctgttttagt tgctcgttcg
    18421 attacacagc cgatcggtga aatgagagaa caagccattc gaatcgctag aggagactac
    18481 agtcggaaag tcaaagtgca tggacaagat gaacttggac agctggctga taccttcaat
    18541 cagttagctg aacgtattga agaagctcaa gatacaatgg aagctgaacg caatcgtttg
    18601 gatagcgtgc tatcccatat gacagacggc gtcattgcga cagatcgcag gggtaaagtc
    18661 attacgatca atgatatggc tgtttccttg ctagatatca aaaacgagga agcaatcgga
    18721 caatcgattt tgacactttt agatatcgaa gaagaatata cgttgcggaa attattagaa
    18781 gatccaaacg aaatggtact tgaccgttca accagtacat tggaaagtga tcagatgatc
    18841 ttgcgagtag acttttctat gatccgtcgc gaatcaggtt ttatcagcgg attagtagct
    18901 gtcttgcatg atgtgacaga acaagaaaaa acagaacggg aacgtcgtga attcgtctct
    18961 aatgtttccc atgaacttcg tacaccgttg acaagtatgc gtagttatat cgaagcattg
    19021 agcgaaggtg cgtggaaaga cgaggaagta gcaccaaatt ttttgaaggt aacgctagat
    19081 gaaacagacc gaatgattcg aatgatcaat gacctcttaa atctatctcg catggataca
    19141 ggtaataccc agcttcaatt agaatatgtc aatttcaacg agttagtgaa ttttgttctt
    19201 gaccggtttg atatgatggt gggaaatcag gaaaaaaatt ataagatccg tcgtgaattt
    19261 acgcaaagag atctttgggt cgaagtagat acagataaaa tcatccaagt agtcgataat
    19321 atcatgaata acgcgatcaa gtattcacca gatgggggaa cgattacttg tcgcttatta
    19381 gaaacgcata ataacgtgat tttaagtatc accgatcaag gattggggat tcctaaaaaa
    19441 gatctgaacc gagtgttcga gcggttctat cgagtagata aagcacgtgc aagagcacaa
    19501 ggtgggacag gacttggatt agctatttct agagaagtga ttaaggcgca tcgaggcgct
    19561 atttgggcag agagcaagga aggaaaaggg tcgacttttt atatctcgct gccatatgaa
    19621 ccttatgagg aggaatggtg ggaatgagaa tctctgaaaa agtcgttcgt ataggactca
    19681 ttttgatggt tgcgttaagc atatacttct cttatgcaat ctggctgagc ccagcaggaa
    19741 aaagttcaat caacgtagat gagacaaaca ctcaaatgat cgagtctcaa agcaatcgaa
    19801 aaacttccga agtctttctt ccgcttcatg ttacatggca ccattcggga atgatccaag
    19861 aaaccaacag tgaaaatctc gtgtaccgat tgcaattgat catcgataaa gcaagatttg
    19921 gaaagctttc tgaagtagtt tcaggagaca aatcacagtt tgaacaggtg aaaaatctga
    19981 actctggttt tgagttggcc tataatgcgc cttttttgct aagagaatac aaggaagcat
    20041 ttgatttaga tttagatgtg tcgaacttgg caaaagaaag cgaaaatatg tattttacta
    20101 gaatacaatt cgatcaagga aataaaaaaa tccgattttt gaattattcg aaccatctcg
    20161 tatatgaagc aaatatttcg ttgaattgga cagatatcaa taaagaatta acagcaaaag
    20221 acacaaaatg gacagaaatg acaacagaac catcgacgat cgacacgcaa tatgctacga
    20281 aacattcgat caaactgaag aagtatagtt atatcctgtc acagcaaccc tatacacttt
    20341 ttagaaatgc ttttttccag aaaccagata gcgtgaaaaa taatgatgaa agcaatgaat
    20401 tgatttttta cggtgggaat gaaacaatgt ctatccattc ggaaacgcaa gtagttgatt
    20461 ttcgtggaga gctgccagag aaaaaagaaa atgctagtgt attttctgaa agtttcccct
    20521 atattagcaa attaggcagt tctttaggga acctcagata ttttgaccgc agtgacaata
    20581 cgatcgatta tcggatattc gtggaaggct tcccggtttt tgggacagac agcagaggac
    20641 agattggtgt ggaagttggc ggtgaaacga atggttctgt gcaagtgaat atccaaacca
    20701 gtttgaatac gattcaagtg ccgatacctt cagacgaaga agtagaactt gcaagtacga
    20761 atgaaatcat ccagcagtta aaagctaagg gagcagatag caacaagatt ggttcagtga
    20821 tcgttggcta tacatggcaa aatgtcaaag aaacgaatcg tgtcgttgat ctcttacctg
    20881 aatggtatgt gaaatataaa gataactggt attcagctaa cgaccttctg gaaaaattac
    20941 ctggattgga ggcgaattaa ttggatttca aaaaaatcga atggatattt ttcgtcgctt
    21001 ttttagggtt gaatgtgttt ttattcagca gttatcatga cgcggtatac caagaaaaca
    21061 atgtcattcg ctctaatcag actgaatcaa tccaacaacg tctggctagc gacgatatca
    21121 cttacacggg gaagatttcg tctgagaaca agcaaggcta ttatcttagc gcggagcaga
    21181 ccaatcttag cacttcatta gcgaattatc gcaaggctgg taaaagcggc ttgctggcaa
    21241 gtggtgtgtt tttagacggt gatgttttga cgaaatcttt atccagttca gatgctgaaa
    21301 agaacgtgat cgatccagat aaaattagta gttccttgga agatttttta tctaacaaag
    21361 acaatatttt attcggtaat gattatgtgt acacgaaaaa cttttctcat ttagaagaag
    21421 aaccactaga aatccaagca agccaagagt atgaaggcat cccattcaat gatgatacag
    21481 caaaaatcgt tctttcttta gaaaaaaacg ataatgatta tagcatcacg aaatacatgc
    21541 agacgcattt atctgatatc gagcagctgc gcgaaaagac ggaactgtat acggaagagg
    21601 aagcaatcaa tacattgtac atcaataaca aaataccaag aggatcaaaa attttatggc
    21661 gtcagctagc ttattcctgt attctgaaag tccgggaaaa aaatgtgtat gttcctgttt
    21721 ggcatgtagc gatcgaaaca agcgataaag tggtccaaat tgagagcgtc aacgctttca
    21781 gcaatacgat cgtgacaaac aatactgtac caaaagtgga agatcaataa aaaagcgagt
    21841 ataatgaaaa aagtttacaa agtaagaagg gaagtaggag tcaaatggct gaagaaaaac
    21901 cagcttttaa aatcagtatc ctagcaagtg gaagttcggg taattcactt tatatcgaaa
    21961 gtgaaaaaaa acggcttttg gtcgatgcag gacttagcgg taaaaaaatc acttccttga
    22021 tggctcaaat tgatcggaca ccggaccaat tagatggaat acttgtgacg catgaacatc
    22081 gagatcatat acatggcgtg ggtgtcttgg ctcgtaagta tcatttggat gtttttgcca
    22141 atcagcagac ttgggaagcg atggaaccac tgattggaaa tgtaccagtg gaacaaaaac
    22201 atctatttga gatggggaaa gtcagaacat tcggcgatat cgatattgag agtttcggcg
    22261 tctcccatga cgcagcggct ccgcaatttt atcgtttcca taaagaaaat cggtcattcg
    22321 tcatgttgac ggacaccgga tattgcagtg attatatgcg aggtgtcatt gaaaatgccg
    22381 atggttattt gatggaaagt aatcatgatc ttgagatgtt acgcatggga ccttatccgt
    22441 ggaatttaaa acagcgtatt ttaggagata gaggacatct ttccaacgaa gacggtgcac
    22501 ttgtcatgac tgatgtgatc ggtaatcgga ccaaacgaat ctatcttggc catcttagca
    22561 aggaaaacaa tatgaaagaa cttgctcatt taacaatgga aaatgttttg aaagaaaaag
    22621 attttggcgt tggacatgat tttgaaatct atgatacaga tccagatgaa gcaactgagc
    22681 tattttcgat ttaaacagtt caatcaataa aaatataaaa agcagaagat aaaatcaatc
    22741 agggttggtt ttatcttcta tttttatttt tttagcatat taaaaaaata accgtattat
    22801 cgactgacta cactgatgtg ctgcttttca tgtttctcat ccagtatctc atctactaat
    22861 gcaatcgcgt aatctgccat gctgatcgta ctgtcgctag cagcgtttct ttgtaaataa
    22921 tcatcactta acaaataagt acctgtacgt ttgccatgtg gatcaaataa agaagctgga
    22981 ctgatataag tccaatgtag atttttagat tgtttcaatt ttaacaatcc ttcgtacata
    23041 ttgaaagctg ttggatagaa gggtgcattt ttaggtactt ggtcaatcat acgagtcgtc
    23101 ttttctgtat ctaagaataa cgaagaggca cccccaacga caattaagcg tgtatcggtt
    23161 ccttcaagca tctcaatcaa atgggacaaa gaagtttgat gcatttcttc ttttcctttg
    23221 ggagcgttga aagcatcaac gacaacatca aaagatgcaa gatcagtagt tgtcaactca
    23281 aataaatcct tttgtataaa cggaacatct gatggcaatt tttctggatg acgcacgatt
    23341 gccgtcacgt ctaaatctct ggacaatgct tcgtctaaaa taaatgaacc tgcatggcca
    23401 gtagctccaa tgagtgcaat tttcataaaa aacctccttt tttattcctg tccattgtaa
    23461 gcagttgctg aatattcgtc aaagaatccg atttttgaaa agctcagtgc gcattccttt
    23521 catggttcgt gatcattggg tatagttaaa ttaagaaaac tgattgataa aaatagaagt
    23581 tgaaggagat aagaaatgaa agagtggtta gatcgtgcct ttttaccaat gataacatct
    23641 gttgtgaatc ttagaccctt aactgctttg cgtaatggaa tgacttacac gatcccaata
    23701 attattgttg gctcgctgtt tttcttattt gctcattttc ctgtggatta catcgctcag
    23761 tggatagaca aaacaggatt ttctatgtat tttacacaag tataccaggc tacatttgat
    23821 gtgatagcct tttggatcgt ttttgccatt gcatacgctt atgcaaaaga agaagacatc
    23881 gatcgcttac cagtcggcat aacagccttg tctgcttatc tactgcttat tcagccacta
    23941 aatactcaga tagatactgt ctggacaggt gctgaaggga tactaggagc agttacaact
    24001 gggctcatca tcggatgggg ctatgcacgt ttagtaagac ttgcgaaagg gtggaagcaa
    24061 agagagaaga tacctgaaaa tgtttggcag tcttttactt cattgattcc aatcattgtt
    24121 atagtcactt ttacgcttat cggttcagcg gtttttcaac atttgttcag aatgactgtt
    24181 gttcaaggag tatggacata tgtccagctt cccattcaat cgttcataga cacgttgttt
    24241 ggtgtgcttt tattaggttt ttttgttccg tttttatggt tatttggtct tcatggatct
    24301 gcgatggtca atgggttggt tagtcccgtc ttgcaagcga attcattagc aaatgcagaa
    24361 atcttagcct caggacaaga gttgacggtg gcaaatggcg gacatatcgt tactcagcaa
    24421 tttctcgatc agttcatgac ggttacgggt gctggattga cgttaggggc tgtatttttt
    24481 atgacggttt ttgcgaaatc gaagaaatac agagaattag gaaatcttgc tcttttacca
    24541 gcctttttca acatcaatga atcaattatt ttttccacac cgatcgtgat gaacccgatg
    24601 atggctgttc cttttatttt cgcaccggtc gtttcaggat tgattaccta ttccgcttta
    24661 tattttggat tcgttccttt atttacagca gtccaagtcc cttggacaac accgccgatc
    24721 ctctcgggtt tcttagtagg cggagtgcct gcagcgattt tacaaggtat cgtgttgtca
    24781 attagttttt tcatttattt cccatttttg aaaaaaatag attctgcaaa ctgcaaaaag
    24841 gaaagacaga caaaaaatga tggagcagca gaaaagatga acgactaaaa aacaaaaata
    24901 ctcctgtcgc tttagattgg gaaaatcacc aatctaggat gacaggagta tttatagtca
    24961 tgtttatact agcccttttt ggacagctaa ttgaacaaga acaactaatg tgttcattcc
    25021 tacgtgtgtg atcatcgatg tccagatccg accagttgct ttgtactcta ggctgaagaa
    25081 gaaacctagg aaaaagtaga ttagcagatg accatcatta tgagcaaacg caaacagcaa
    25141 agagctgatc actgctccga cccaaaaatt tgaatatcga ctgatgatcc ccagtatact
    25201 gcggcggaat acgaactctt ccatgatggg tccagcaaca gttgtcgcca aaataaaagc
    25261 gggtgcttcc atgatcatct ggatgatatt ttgtgtattc tcggaagggg ttgctttgcc
    25321 aaacaataca gattctaaca tgacagcgat gctttgcaga atcaatgcga caaagattcc
    25381 aaccaatcca taaataacaa tctttggagc agaagctttt tttaccgctg attcgatcgc
    25441 taagggttca cgttgcttaa aataaaaaat agctaggaca acggcgccta gtatatacat
    25501 gactgcagtt gctgtgatca attcagacgt agattttaca agtcctatcg aagctaggat
    25561 caagggtgag ataaaaatta gaccatagca aaaaatgctg aggaagctat atttttttaa
    25621 agacatgtaa ttcctccttt taagggataa caagcatgca aggcaagctt atatcgattg
    25681 atttcaatta tatcataatt tcaataagca gctgtcagaa taaaatttct tttctttgat
    25741 ttaaaagctt gcaaaaaaga gggagaatga gtattattaa aaatgtaggt tagcactcga
    25801 aggttaagag tgctaacaga ttaaaaaaag agttatcgga gggatttatc gtgttaaaac
    25861 cattaggtga tcgcgtcatt atcgaagtcg caaaagaaga agaaaaaact gtcggaggaa
    25921 tcgttttagc ttctgctgct aaagagaaac cccaaactgg gacagttgtt gcagtcggtg
    25981 aaggccgttt gttagaaaac ggtgaaaaag ttcctgccgc tgtaaaagca ggcgaccaag
    26041 ttatgttcga aaaatacgcc ggtacagaag taaaatacga aggaaaagaa tatctgatcg
    26101 tcgcaggaaa agatatcatg gcgattgttg agtaaatttg gaagataaaa aaataatcaa
    26161 aaataatcaa tgaggtgaat caatcatggc aaaagaattg aaatttgcag aagacgcacg
    26221 cgcagctatg ttacgcgggg tagataaatt agcagataca gtgaaagtaa cattaggacc
    26281 taaaggccga aatgtcgtac tagaaaaatc ttacggctct ccattgatca caaatgatgg
    26341 tgtgacgatc gcaaaagaaa tcgaattaga agatcatttt gaaaacatgg gtgcaaaact
    26401 tgtatcagaa gtagcatcaa aaacaaatga tattgcagga gacggtacaa cgacagcaac
    26461 tgttctgaca caagcaatcg ttcgcgaagg tttaaaaaac gtaactgcag gtgctaatcc
    26521 tctaggcatt cgtcgcggga tcgaattggc aacaaaagca gcagtcgaag aattgcataa
    26581 tatctcaact gttgttgatt caaaagaagc cattgcacaa gtagcagctg tttcttcggg
    26641 ttctgataaa gttggacatt tgattgctga tgcaatggaa aaagttggaa acgacggtgt
    26701 catcacaatc gaagaatcaa aagggatcga aacagaacta gatgttgtag aaggtatgca
    26761 gtttgatcgt ggttacttat cacaatatat ggtaacagat aacgacaaaa tggaagctgt
    26821 tctagaaaac ccatatatct tgatcacaga caaaaaaatc tctaacatcc aagatattct
    26881 gccattgtta gaacaaatct tgcaacaatc acgtccattg ttgattattg ctgacgatgt
    26941 agatggtgaa gcattgccaa ctcttgtatt gaacaaaatc cgtggaacat tcaacgtagt
    27001 agctgtaaaa gctcctggtt tcggcgatcg tcgtaaagca atgcttgaag atatcgctat
    27061 tttgacaggt ggtacagtaa ttactgacga tctaggttta gaattgaaag atgcaacaat
    27121 cgaaaactta ggaaatgctt ctaaagttgt tgtagataaa gacaatacaa cgatcgtaga
    27181 aggttctgga gaaaaagaag caatcgaagc tcgtgtgcaa ttgatcaaaa accaaattgc
    27241 tgaaacaact tctgattttg accgcgaaaa attacaagaa cgcttagcta aattagcagg
    27301 tggtgtagct gttgttaaag ttggtgcagc tactgaaaca gaattgaaag aattaaaatt
    27361 acgcattgaa gatgcattga atgctacacg tgccgcagta gaagaaggaa tggtctcagg
    27421 tggtggtaca gctctagtga acgttatcag caaagtttct gctgtagaag cagaaggaga
    27481 cgtggcaaca ggtatcaaga tcgttgttcg tgctttagaa gaaccaattc gtcaaatcgc
    27541 tgaaaacgct ggttatgaag gatcagtgat tgttgataaa ttgaagaacg tggaactagg
    27601 cactggtttc aatgctgcta ctggagaatg ggtaaacatg gttgaagcag gtatcgtaga
    27661 cccaactaaa gtaacacgtt ctgccttgca aaatgccgct tccgtttctg ccttgttatt
    27721 aacgacagaa gcagtcgttg cagataaacc agaaccagca gcgccagcag ctcctgcaat
    27781 ggatccatca atgatgggcg gtatgatgta gtaattaaga aagaacccta ttttaacagg
    27841 gttctttctt ctttttttgt tttattttaa gcaaaagggg caaagaaggg gcaaaaatat
    27901 caaaggctat ttagtttatc aattacttgt tgtttggctc tttttgttac atggttataa
    27961 atagacagag ttgtgtttgc atcagaatgc cctacacgct ccataatggc tttaagaggt
    28021 acgcctaatt cagataatag tgaaacgtgg ctatgtctga atatatgtga ggatagactt
    28081 ttttctaatt ccaactcttc ctctacttta tggagtatag cgttaaatga gtgcagcgca
    28141 agcggagtgc cacttgtaga tataaatata tattgatcgg gatctgtggg tctacctgca
    28201 agaatattgt cagctatcac actttcaatc aattcttttg cacgattggg taattgcact
    28261 tcacgttgcg aataagtatt ttttggagtt gtttttatag cattatccat tttcacagat
    28321 gtgtaatcta aggtcccatt aatggaaatt ttcccatcct catagtcctt catttgcaaa
    28381 gctagcaatt ccccatatct caaaccagtt aaatatagaa actcagctat tatgccgtgt
    28441 agttttctgc gaggattgga gtatagctgt ttcagtattt gatcaatttc ttctttctcc
    28501 agatattttt tattcataga aagccttctt ttttcttctt ccactttttt aggatggatt
    28561 ttaactgcta gcgcagggtt tctttgaatg tattttctat cgattgcata gtttagcata
    28621 acagatagag ttgtttttgt ttgttttgtg tagttcagtg agaggtcacc aaacgtatac
    28681 atatcttcaa ttatcttatt aatgagtgtc tcatcaatgt ttctaacgat cgtatcatcg
    28741 cttatgtgct tagaaacatg tttcatcatc attggaacct tcaaatagct agtacgttta
    28801 acatgctgct tataatattc ataccattct ttatacagct caccaaaagt gatatctgat
    28861 ttattgtaat cttcgagtgc ttctttaatt tttttatcta gaattttctg agcttttttc
    28921 cacgcttgtg gtgaattact tgtaagtgtt gtagattttt ttcgtgtttt ttctgtataa
    28981 ggatctacat atctttcaat aaacttgaat tggccatctt tggtctgttc aacccacact
    29041 tttaacatct cctatcattt gctataatag gcataacaaa tagacctata taggtttgtt
    29101 ttctaaaagc acgctcttac tctggacggt ggggcgtgtt ttttatttat tatacagttg
    29161 ctttaaaaat agctgctttt tcaaatgata ctaaaggaga gaagtggatt tctatttctc
    29221 ccttttggtt taacgtgaaa tgagcagtta catccatttg ttttcctgga gcaacagatc
    29281 ccattgtatt ttcgttggca tatgtctctg attttttgtc atctggtcca tatacttcca
    29341 catctgtacc tacagggata tctgaatcgc catcattttt tacggtataa gtaattttta
    29401 ctacttgtgc aggttggttt tcttcaaatt gatttctttc atcagttagt tctacactat
    29461 ttagtgtata ttcagcatca ccaactttta cagtgtcacc aatcttatag aaggtatcgc
    29521 ttttttcttc tttagaagat gaagcagagg ttgaagattt agttactttt tcgccaccat
    29581 tatcacttgc tttattacta tctgatccac catttaaagc agaaccaata attataatta
    29641 aaattactgc taatacccaa aaccatactt ttttgtaaaa aggtttacta actttataca
    29701 tttttccgtc ttgacccata actttttttg ccatttaaat attcctcact tcttgttata
    29761 atatatttgc gatctcagaa atgaggtatg agtccgtgtg ccagcacggg cttttttaat
    29821 gttttgctga aatcggtttt tttaataatt cttgatgtat tcatacatat tgccttgcgt
    29881 aagaatattt tttcttaaaa tagcattggc agacagcata acaagtgtat ctgagcttat
    29941 tctgaataga ataatactcc ataaattttt cgagattaaa ctgagattca tcagtcagtt
    30001 cgttctcaat atagatattt aataaaatta gaatagctat tttatctgct tctgtttcaa
    30061 attttgagtg aaaagttgta gaagtatcgt acaaaactga atattcaaaa tgagaagcgg
    30121 tgaaatgcgc aagttcatga gataaatgaa aggcttctgc agtttcgcca tatagatttt
    30181 cattcaaaaa aatgattcta ggcttcggat aatagaagcc aggctctttc atttccatgt
    30241 aaactacttt caaattgtat tcgctcaaca tttctttcaa tttcaaatac atacaaccca
    30301 tcactccaac tattcatttt cctctaaagc tttagcaatt gcaatcgctt tacgcattgt
    30361 ctccttagat atttcttttc catcaaaaga aaaaacagta tcgttttctg ataaatccac
    30421 atgtttaggg gtttctcttt cttctctacc tagaaggtag tctacagaga ctccaaagta
    30481 atcagctaac cgagctaact caatagaatt aggagtttgt cttttccaac ttcctaaata
    30541 tccattcgaa tacccgaaat tcatttcgag ctgtctaatg gataatcctt tttcttttgc
    30601 taactccttt attatttcgt aagtattcat tgataaatca acctttctga atgcttacaa
    30661 aaaaagttta gataaataat gtaaaaatag
//
LOCUS       DARBFO010000024        30469 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000024 DARBFO010000000
VERSION     DARBFO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.025"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..107)
                     /locus_tag="SCK51_001221"
     CDS             complement(<1..107)
                     /locus_tag="SCK51_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG1251859.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVY"
     gene            complement(109..306)
                     /locus_tag="SCK51_001222"
     CDS             complement(109..306)
                     /locus_tag="SCK51_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEG1251860.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(700..2427)
                     /locus_tag="SCK51_001223"
     CDS             complement(700..2427)
                     /locus_tag="SCK51_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEG1251861.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2420..3613)
                     /locus_tag="SCK51_001224"
     CDS             complement(2420..3613)
                     /locus_tag="SCK51_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1251862.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3800..4444)
                     /locus_tag="SCK51_001225"
     CDS             complement(3800..4444)
                     /locus_tag="SCK51_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1251863.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4538..4672)
                     /locus_tag="SCK51_001226"
     CDS             complement(4538..4672)
                     /locus_tag="SCK51_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEG1251864.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4674..6806)
                     /gene="feoB"
                     /locus_tag="SCK51_001227"
     CDS             complement(4674..6806)
                     /gene="feoB"
                     /locus_tag="SCK51_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEG1251865.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6803..7276)
                     /locus_tag="SCK51_001228"
     CDS             complement(6803..7276)
                     /locus_tag="SCK51_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEG1251866.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7677..7901
                     /gene="nrdH"
                     /locus_tag="SCK51_001229"
     CDS             7677..7901
                     /gene="nrdH"
                     /locus_tag="SCK51_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEG1251867.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8060..10219
                     /gene="nrdE"
                     /locus_tag="SCK51_001230"
     CDS             8060..10219
                     /gene="nrdE"
                     /locus_tag="SCK51_001230"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEG1251868.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10269..11234
                     /gene="nrdF"
                     /locus_tag="SCK51_001231"
     CDS             10269..11234
                     /gene="nrdF"
                     /locus_tag="SCK51_001231"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEG1251869.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11323..12462)
                     /locus_tag="SCK51_001232"
     CDS             complement(11323..12462)
                     /locus_tag="SCK51_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1251870.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12771..13484)
                     /locus_tag="SCK51_001233"
     CDS             complement(12771..13484)
                     /locus_tag="SCK51_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEG1251871.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13738..14439)
                     /gene="nagB"
                     /locus_tag="SCK51_001234"
     CDS             complement(13738..14439)
                     /gene="nagB"
                     /locus_tag="SCK51_001234"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEG1251872.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14673..16205
                     /locus_tag="SCK51_001235"
     CDS             14673..16205
                     /locus_tag="SCK51_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1251873.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16396..17598)
                     /locus_tag="SCK51_001236"
     CDS             complement(16396..17598)
                     /locus_tag="SCK51_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1251874.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17680..18369)
                     /locus_tag="SCK51_001237"
     CDS             complement(17680..18369)
                     /locus_tag="SCK51_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG1251875.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18475..18843)
                     /gene="rplL"
                     /locus_tag="SCK51_001238"
     CDS             complement(18475..18843)
                     /gene="rplL"
                     /locus_tag="SCK51_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEG1251876.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18896..19399)
                     /locus_tag="SCK51_001239"
     CDS             complement(18896..19399)
                     /locus_tag="SCK51_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEG1251877.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19603..19730)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19809..20498)
                     /gene="rplA"
                     /locus_tag="SCK51_001240"
     CDS             complement(19809..20498)
                     /gene="rplA"
                     /locus_tag="SCK51_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEG1251878.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20602..21024)
                     /gene="rplK"
                     /locus_tag="SCK51_001241"
     CDS             complement(20602..21024)
                     /gene="rplK"
                     /locus_tag="SCK51_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEG1251879.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21367..22035
                     /gene="sdaAB"
                     /locus_tag="SCK51_001242"
     CDS             21367..22035
                     /gene="sdaAB"
                     /locus_tag="SCK51_001242"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEG1251880.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22048..22920
                     /gene="sdaAA"
                     /locus_tag="SCK51_001243"
     CDS             22048..22920
                     /gene="sdaAA"
                     /locus_tag="SCK51_001243"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEG1251881.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23011..24030)
                     /locus_tag="SCK51_001244"
     CDS             complement(23011..24030)
                     /locus_tag="SCK51_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEG1251882.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(24203..25108)
                     /locus_tag="SCK51_001245"
     CDS             complement(24203..25108)
                     /locus_tag="SCK51_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1251883.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(25167..25712)
                     /gene="nusG"
                     /locus_tag="SCK51_001246"
     CDS             complement(25167..25712)
                     /gene="nusG"
                     /locus_tag="SCK51_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEG1251884.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25813..25983)
                     /gene="secE"
                     /locus_tag="SCK51_001247"
     CDS             complement(25813..25983)
                     /gene="secE"
                     /locus_tag="SCK51_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEG1251885.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26007..26159)
                     /gene="rpmG"
                     /locus_tag="SCK51_001248"
     CDS             complement(26007..26159)
                     /gene="rpmG"
                     /locus_tag="SCK51_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG1251886.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26321..26965
                     /locus_tag="SCK51_001249"
     CDS             26321..26965
                     /locus_tag="SCK51_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1251887.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27021..27524)
                     /locus_tag="SCK51_001250"
     CDS             complement(27021..27524)
                     /locus_tag="SCK51_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEG1251888.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27537..27583)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28121..29086
                     /locus_tag="SCK51_001251"
     CDS             28121..29086
                     /locus_tag="SCK51_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEG1251889.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29185..30357)
                     /locus_tag="SCK51_001252"
     CDS             complement(29185..30357)
                     /locus_tag="SCK51_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1251890.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30344..>30469)
                     /locus_tag="SCK51_001253"
     CDS             complement(30344..>30469)
                     /locus_tag="SCK51_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1251891.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         9297 a         6262 c         5400 g         9510 t
ORIGIN      
        1 aaatatacct tttgtaaaac ctcaagaata tcagaatatt taggatcttt acttatagat
       61 atatcattat ataattcatg aacaatttgc ttagcatttt ttttcatttt agcacttccc
      121 tggaattgct ccacctaaaa aaccacctat agacattcct gcaatacaag tagttgcctt
      181 ggcccaatcg accgtacatt tatttttagt gcaatacact ccatttccat aatattttcc
      241 actatgagtg gtacccccat agataagttg tgtctcttta atagacaaaa tttttaaatg
      301 tttcataatc taatcacccc taaattaatg tttttagact attattctaa cattcattaa
      361 atttcaaaaa acaattcctt cttgaaacgt agcaaaataa ttcctgaata agaatttaat
      421 cacttttaag tatataaact atgtatattc tttaatattt tttgagaatg cacttcgtac
      481 ataataaact ttaatcacat tagtctttca tacctacgat cgatctcttt gactcgcacc
      541 cataaattca taatagtttg tcggcgtctt tggacgtttc gaagatgagc tacgcattct
      601 ctttttttat agacaaaaaa gatagagacg aacatggagc ttccaaattt ggatatttga
      661 tccaattttg gaagctccat ttatttcgtg tctgtctttt tattttttcc agctttcgac
      721 taattcttgg ttctctttga tccagtcttt tgctgcttgc tgtggatctt ttccattgtt
      781 gatttccaac ataacttttt ccatatcttt ttctgaccaa tggaaattgt ctaatacttt
      841 gtaagcttct ggctggtctt ctttcaatcc ttttcgaacc atcgtatgga tctgctcttc
      901 gcttcccatt ccgttttcag gatcttctaa gtatttcaga tcatatttgg caaacatcca
      961 atgaggtgtc catcctgtaa ctacaattgg ttcgtgtttt ttgatcgctt gacctagtgc
     1021 aacggtcatt gctcctgaag aagatgtctc tactttccag tctttcaagt tgtcatattt
     1081 ttggatcgtg ttttcagcag ccgtaactac accagctcct ggctcgattc ctatgatttt
     1141 cttacctgcc tgatcggtta actcatcgat cgaatttaca tccatgtagg ctggcactgc
     1201 taaaccaact tttgctcctg tcaaattggc acctaggtct tcgacttgat ctttatattg
     1261 tgcatattgt gaaccatgag ttttaggtag ccaagctgaa accatggcat ctgactcgcc
     1321 atttgaaaca gatttccaca tgattgaatt atctaatggc gtcatcgtta catcatagcc
     1381 catttgtttc aatacttcac cgactacatt tgttgaagca acttctgtat cccattcaac
     1441 ataagataag ttgatacgtt ttgtttcttt ggcagatgaa aaactcccta taccaatcgc
     1501 acctaagatc actactacta cagcaccaat gatcagtcct tgacgttttt tacttttctg
     1561 tgttgttgct cctgcagctt ttttattgtt tggctggttc aaatgctgtg taaaacggtc
     1621 aatgatgatt gctaagataa ctaatgcaac cccgttgaca aatccattcc cgacttgtgc
     1681 tctttgcaat gctgataata cgccgcgtcc taagccgggt gcaccgatca tagaggcaat
     1741 aacgaccatt gaaagtgaca gcattgttgt ttgattgatc cctgccatga tcgtactttt
     1801 agctaaaggc aattctagtt tgaatagttt ttgacgactt gtgctcccga atgaatcaga
     1861 agcttctact aattctttag gaacttgtcg aatccctaaa ttcgtaaaac gaacggttgg
     1921 tggcagagca aagatgactg aggcaaatac ccctggaacc atcccgatac cgaaaaatgc
     1981 aactgctgga atcaaataaa cgaatcctgg cattgtctgc ataaagtcaa ggatcggttt
     2041 gataatactt tgggctttac tgctttttgc catcaagatc cctaatggta ccccaatgat
     2101 gatcgagatc acactagata aaagaacaag tgtgacagta ttcatcaaat cattccatag
     2161 accttggtta taaataaacc atagaccgat taatgtgaac aacgtaagtc ctggacgttt
     2221 cttagaaata aagaaagctg ctaccgtcaa taacaagata aacaaaggag ctggaatcac
     2281 taacagtaaa tttgtaatcc ccgacatcac tgcctgtcca atcgtttgta aaaatgaaaa
     2341 taggccagaa aaagttgtcg taaaccattc tgtgatattt tctacccagt ttgccactgg
     2401 taattgatag ttcaatagat tattcatgtt cactcacctc gcttgtttct gctaatgctt
     2461 caatcacact acctcggatg acgacacctt ttaattttcc atcctctaca actgcaagtg
     2521 gagtcggtga gtcataaata atattgaaaa tatcacttac caccatgtct ttcccaatca
     2581 ccgtcacatt ttcatctagg aagtcgatca atggacgttt ttgcttccta gcttctaacg
     2641 cttgatctgc agtgatgatc cctttcaggt gtctctttct gtcaacagcc atcagcatgc
     2701 tgacttcttc attacgcatc cttgtcaatg caacgtttgg tccatcagat tcgatattcg
     2761 tcgtcaatgc aggtaccatg atgttttgag ccgtaagcac tttggaacga tcgacttctt
     2821 ccacaaattc acgtacataa tcatttgctg gattggtcaa tatttcttca cctgttccga
     2881 tctgcatgat ttcgccgtct ttcatcaagg cgattctgtc cccaatccgt aaagcttcat
     2941 tcaaatcatg ggtaatgaaa ataatcgttt tttgaacatt ggcttgcagg tccagtaatt
     3001 cgtcttgcat ttctcgccgg atcagcggat caagagccga gaacgcttcg tccatcagca
     3061 agatctcagg atcatttgct aaagctctgg ctaggccaac ccgttgttgc atcccaccag
     3121 ataactgact aggatattgg tctttaaatg aaagcaaact agaattttct aatgcttttt
     3181 ctgctttttc ttgtcgttct tctttaggta caccacggac ttctaacccg tattctgtat
     3241 tttccaagat cgttcgatgt ggaaacagac cgaagttttg gaaaaccata ttgattttat
     3301 gacgacggac ttctcgcaat tcttctttgc ttaacttcga tacatcttct ccatcaatat
     3361 agatactgcc cgaagttggc tcaatcagac gattcaatag acggatcaat gtggatttcc
     3421 cactcccgga aagccccatg atcacgaaga tttctccttc ttttacgtca aaattcacgt
     3481 catacacacc aaccgttgca ccggttttct ttaaaatctc cgttttcggc tggtgatcat
     3541 tcatcatatc taaagcggct tgtgtctttt taccaaatat ctttgttaaa tgttttactt
     3601 ctacttttac caaacaaatt ctcctttttt atgtaaattc atttttcttt ctgaaaaaag
     3661 tgaccacact atgttaacat catgtagtca ctttgtcaaa aatgaaagaa atttgcttgt
     3721 ccaaactgaa aaaaacttgg ctaaatcttt gtaaataaaa ggtttagcca agttaatatt
     3781 tttttaatat ttcacacaat cattctgcaa gctgatgctc cgggtaaaaa aagtttgtca
     3841 tacgctgtag tgtatattca tttccaataa aatgaacgat gtcccctgcc gtaaatttag
     3901 cataggggcc aggtgaaaca agcagttctt cattatgaga aattgcgata actgtggcgc
     3961 cagtcgcctg ccagatattc aactcgctaa ttgaacggtc cagatgcatt gctttttctg
     4021 ataaagataa ttcaaacgga agtaaaggat tcatcttatg tattcgcttc gtttgattga
     4081 ccagctgatc caataattga gaaaaatgat tcagctcatc ttgttgcttc tccacacttg
     4141 ccatgatttc tgcttttgta tcttggatgg attgtacatc ttgatattgc aataggaaat
     4201 cttttgcttt ttccttcgaa gcaacaaatg ccccacttcc atgccgtact tccatgattc
     4261 caagatctac cagcacattg atagccttgc gggtagtctc aggagaagca ttgtacgtac
     4321 tggcaagtgt tgagcgggca tggatcttct cacccacttt atatcggttt tcaacgatac
     4381 gttcggctaa atcaacagca atctgctggt agcgcgggcg gacgattttt ttctttccag
     4441 acattttctt tcctctttct ttttttactt tccctcatct tactatgctc tagcaaaata
     4501 agcaaaaaag gggagaatag cctcttgcaa cgactgatca tcgcttgatc tgttcctttt
     4561 ttactgggca agctgtttga cagtcatcac aattcttacc ggattttaaa cgagtataga
     4621 caattgcgcc tgcacttcca aagattaaca cacttaataa gaatgtcgcc atcctattcc
     4681 tctccttcta atgacaatat agtataaaac tggttcttgg attttggctg acggaacaaa
     4741 acataaacga gtcctatcat cagtaaaata gcaagatacg tccctaacgc tacctgtcca
     4801 ccctccaata agtgtcccaa ctgataaatc acaaaactaa ctaaataagc taaaccgcat
     4861 tgatagccta tggcgatcca agtccatttc ttggcattca tttcccgatg gatagcccca
     4921 attgccgcaa aacaaggcgc acaaagtaaa ttgaaggcta acaatgaata agctgctgcg
     4981 ggagtatatg tgtgttgtag cacttgccag atttctctgc cattttcgga tacttccgca
     5041 tgaccaaaca aaataccaaa tgtgccaata atattttctt tagcgaccaa tcctgtaatc
     5101 gctgcgactg ttccttgcca gtttccccaa ccaagcggct gaaaaacaac tgccaggatt
     5161 cgcccgaaaa aggctaagat actttggtct tcagaaacag gttgagcata aaaattataa
     5221 gaagagctga accagatcac gatactagaa acaaagataa tcgttcctgc ttttttaaca
     5281 aaagacttgc tgcggtcata ggtttgtctt aggaccgctg accattgagg aaaatgatat
     5341 gcgggcaatt ccatgataaa aggagctgta tctccagcaa acaaacgtgt ttttttcaag
     5401 ctgattccag agaaaataat tgcagtcata ccaagaaaat aggcagaagg agctacccaa
     5461 gattgatgag gaaaaaaggc ccctgctact aaggcaataa tcggcaactt tgcagaacaa
     5521 ggcatgaagg ttgtgaccat gatcgtcaaa cgacgatctt gctcgttttc aatcgtgcga
     5581 ctggccatga cgcctggcac tccacatcct gtagcaatca gcataggaat gaaggatttt
     5641 cctgaaagac cgaatttcct gaaaagtcga tccatgacaa aagcgatcct cgccatatat
     5701 ccgcagtctt ccagaaagct caagcataaa aacaatataa tcaattgcgg tagaaatcct
     5761 aacactgctc ccacgcctgc gatgatgccg ttcaagatca gctcctgcat ccatcccgct
     5821 acttgccatt cagctaacaa tctgccaaca tgttctggta accatgtacc aaacaatgtg
     5881 tcattgatcc aatctgtccc cattgttcct actgtttgaa tagccagata atagatcagc
     5941 cacatcacaa atgcgaagat cggcaaggct aaccaacgat tcgttgcaat ctgatcgatt
     6001 ttgtcactca tcgataattg gaaagaatca tttgacacaa cacagagtgt acatagttgg
     6061 gtaataaatt cataacgttc attgaccaga atcgtctcac tatcgtcgtc caatagtttc
     6121 tctgttaact ggatcagttg ctcgatctct ttttgctgag aaactgctat ctcgtaatcc
     6181 tctcttacct tctgatctcg ttcaaaatat ttcattgcat accagcgttt cttgccgctt
     6241 tgttctccta gtacatcttc aatttcatgt aaagctgctt ctaaacgtgg atcatatgta
     6301 cagggaacag gaatagaagg ccgctttttt gcagactcct gaatcttttc gattagtttt
     6361 tcgattcctt gctttttcaa tacgctgatt ggtaaaactg gtacaccaag accataggaa
     6421 agcttttcta gattgatttt ttttccttct tttttgatga catccatcat attcagacca
     6481 atgatcatag gaatgtctaa atccaataac tgcgtagtta aataaagatt tcgttctaaa
     6541 ttggtagcat caataatgtt gatgatcagt tcaggctgtt cctgaaccaa ataatctctt
     6601 gcaatcactt cttctggtgt ataaggagac aaagaataga tgccgggtag gtcctgaata
     6661 tgaatgttcg gatttttttt caacgatcct tctttttttt ctactgttac gcccggccaa
     6721 ttccctacat attgattcga accagtcaag agattgaaca agctagtttt cccgctgttt
     6781 ggatttccga ctaacgcgat attcattctt tttcctccct ttgtatcaag attagttcgg
     6841 cctctgactt tcgtaacgtc agttcatacc ctcgcactgt cagttcgatt ggatctccca
     6901 gaggggctag ttttcggata actattttta catttcgcgt caatcccata tccattaacc
     6961 gtcgcttcac tgcgccttgt ccgtggattg ctacgacgat cccagtctct cctgctttta
     7021 attgatcaag agaagtccag ctttcagagg aagcagtctt tttattgaca aagatttgac
     7081 ctagtatttc ccgacttact gccactcgat gactttttac cagtaaaatg gcattgttgc
     7141 ctgataaatt gacgacgacg attttacttc cggcaacgat cccgatattt tgcaaatggc
     7201 gttttgtttt ttcttctgtt tcgattcgtt cgacttgata aacgaaattc tgctttgcct
     7261 gcgctaaggt ctgcatattg attcctctct tgtgaaataa atcatgtatt aaaatcattc
     7321 attatatgtt ctaattttct tacgtgttac gcaaataaat gagaatcatt ctcacataga
     7381 atactctcgg taattgagaa tgtcaattat acttgtgata ttatgagcga atctttagta
     7441 ttgatagata aaaatgagaa aaaaatattt cttcatagtt ttccacaatt ttttctctat
     7501 taaatttgct attttcccac acgatacttg ttttcagctc ttttttcaaa aacacaaaac
     7561 ttagtattcc acagtttcca caacacaata tgtagtgttt gttgtttgac ttttttacta
     7621 agagttttat actatctatt atagataata agaatgtgtt agaggaggca ctcaccatga
     7681 acgtaaaaat cttttctaaa aataattgta tgcaatgtaa gatggtcaaa cggtttttaa
     7741 cagaaaatca aattgctttt gaagaagtca atatcgatga acagcctgaa gcagtagact
     7801 ggttgaaagc acaaggattc caaagtgttc ctgtcattac ttctgatgca gaaacagtta
     7861 tcggattccg tcctgatcag ttgcgtaaac ttgccagcta acatgactaa aagaatcaaa
     7921 acgtgataca tggatttgaa gggcttgaga cagcaccagt ttttgtcgca aacttccaaa
     7981 tcctttttta aagcttaaaa acaatgcaat gatttgcaat caagcatcat caaatacgaa
     8041 atgaaggttg ggcgtacgta tgagtcttaa aaatttacaa gatgtcagtt atttcaagct
     8101 gaataatgaa atcaatcggc cagtcaatgg tcaaatccct ttgaataagg ataaagaagc
     8161 tttagttgcc ttttttgagg aaaatgtcaa accaaatacg atgacttttc tatctattat
     8221 ggaaaaaatc aattacttga tcgatcaaga atatatagaa gcagaattca ttcaattgta
     8281 ttcaccatca tttattgaaa agctatatca atttttggac gagcaacatt tttctttcaa
     8341 atcttttatg gctgcttaca aattttattc tcaatatgcg ttgaaaacca acgacggttc
     8401 cgcttatctg gaaacttatg aagatcgagt agccttcaat gcgctttact ttgcgaacgg
     8461 ggatgaagaa ttagcacttg ctcttgcaga cgaaatgatc catcaacgat atcagccagc
     8521 tacaccttct ttcttgaatg ctggacgtaa acgtcggggc gaattggtgt cttgtttctt
     8581 gactcaagtc actgacgata tgaactctat cgggcgctca atcaactctg ctttgcaatt
     8641 gtcccgtatt ggtggcggcg tagggattac tttatcaaat cttcgtgaag ccggcgctcc
     8701 tatcaaagga tatgagggtg ctgcaagtgg tgtcgttcca gtcatgaaat tattcgaaga
     8761 tagtttcagc tattctaatc aattaggtca gcgtcaagga gctggcgtgg tttatttgaa
     8821 cgttttccat ccagacatta ttgctttcct ttcagcaaaa aaagaaaatg cagatgaaaa
     8881 gattcgcgta aaaactcttt ctcttggcgt gatcgtacct gataaatttt atgaattgac
     8941 acgtaaaaac gaagatatgt atttatttag tccatatagt gtcgaaaaag aatatggtgt
     9001 cccattctct tatgtaaata ttacagctga gtatgatcat ctagttgcaa atccaaacat
     9061 tcgcaaacaa aaaatcaaag cgcgtgatct tgaaaatgaa atctcaaaat tacaacaaga
     9121 atctggctat ccttacgtaa tcaatattga tacagcgaac cgcgagaatc caatcgatgg
     9181 caagatcatc atgagtaatt tatgttcaga gattttacaa gttcaaacgc cttctgtgat
     9241 taacggcaaa caggaatatg aagtattagg aacggatatc tcatgtaact taggctcgac
     9301 aaatatcgtg aatttgatgc aaagccctaa ctttggtcaa tctgtgcgta cgatgactcg
     9361 tgcattgaca tttattacag atgcatctga tatcgatgtc gtaccaccga tccaaaacgg
     9421 aaataagctg aatcatacga ttggattagg tgccatgggc ttacacactt atcttgcaaa
     9481 agaacaaatg gaatatggct cagaggattc attagacttt acaaatattt acttcatgct
     9541 gttgaattat tggacacttg ttgaaagcaa caatatcgct cgtgaaagaa aacaagtttt
     9601 ccataatttt gaaaaatcag cttatgctga cggcacttat ttcgaaaaat acgtaactgg
     9661 agaattccag ccaaaatcag aaaaagtcaa agcattgttt gaaggcattt ttatcccaac
     9721 tgctgaagat tggaatgctt tgaaacaagc agtaatgaaa gatggactgt atcatcaaaa
     9781 tcgcttggca gtagcaccaa atggctcaat ttcatatatc aacgatacga gtgcaagtat
     9841 tcatcctatc actcgcatga tcgaagaacg ccaagagaag aaaatcggta aaatttatta
     9901 cccagcgcct tatctatcca atgagacgat tccgtattac acttctgctt atgatatgga
     9961 catgcgtaaa gtaatcgatg tttatgcaat ggcacaacaa cacgtcgatc aaggcatgag
    10021 tttgacttta ttcatgcgtt ctgaaatccc agaaggcatt tatgaatgga agacaacaac
    10081 gaagcaaacg acacgtgatt tgaatatttt aagacattac gctttccaca aaggaatcaa
    10141 atccatttat tacgtacgta cctttactga tgatgcagaa gaaatcggaa gcaatcaatg
    10201 tgaaagctgc gtgatctaat atcatagata gattcgctta ttcatatttt aattaaagga
    10261 gcattctcat gtcagaaaca tattatgaag caataaactg gaatgaaatt gaagatatca
    10321 ttgataaatc cacttgggag aagctgacag agcaattttg gctagataca cgtatcccac
    10381 tttcaaatga cctagatgat tggcgtacat tgtctcaatt ggaaaaagat acagtaggac
    10441 atgtatttgg cggtttgaca ctgctagata ctgtccaatc tgaaagtggc atggatcagt
    10501 tgcgtaaaga tgtccgcact ccccatgaag aagccgtttt gaacaatatt caattcatgg
    10561 aatccgttca tgccaaaagt tattcttcta tttttagtac actgaatacg aaaaaagaaa
    10621 tcgaagaaat ttttgactgg acaaatacga atccttatct gcaaaaaaaa gctgagcgta
    10681 tcaatgaaat ctacaaacac ggtacaccgc tagaaaagaa aattgccagc gtctttttag
    10741 aaacattctt gttctattca ggtttttata ctcctcttta ttatttagga aacaacaaat
    10801 tggctaacgt agcagagatc atcaaactaa tcatccgaga tgaatctgta catggaacct
    10861 acatcggtta caaattccaa ttaggattca acgagttacc tgaagcagaa caagaaacgt
    10921 taaaagactg gatgtacaat cttctttatg agctttacga aaacgaagaa cgttatacag
    10981 aagaattata cgatccaatc ggctggacag aagaagtcaa gacattccta cgctataatg
    11041 ccaacaaagc attgatgaac ctagggatgg atccactctt tccagatact gccaatgatg
    11101 tcaacccaat cgtaatgaac gggatttcaa caggcacaag caaccatgac ttcttctcac
    11161 aagtcggaaa cggctatttg ttaggtcatg tagaagcgat gaaagacgac gattatttga
    11221 ttggattgga ataaggattt attttaattc aaaaattttt cgaaaaataa aaagagttta
    11281 gcgtacagat attttaaatg tacactgaac tcttttttat gcttattcac ttgtttcttc
    11341 cagcactttt ggactgtcta acttttccga aacatgttcc attcttctca ataaatagat
    11401 tttccggata tttaatatta tcgtcttaat cactgcatag acaggtatgc aaatcagcat
    11461 gcctaatatt cctgcgacac ttcctgctcc aatcaatacc aagatgatcg tcaaaggatg
    11521 gatattcatt gatttcccaa ataacaacgg cttgatcaaa tttccatcta attgttggat
    11581 cactaaaatc gacaacgcca tatacaaagc ttgaaatgga gagataaaca aaccgacaat
    11641 gattgctggt accgctccaa taaaagggcc gacatacggg ataatattcg tgattccgca
    11701 aatgaccgca agcaacaagc tataaggctg atcgaatatt ctcattgcca aaaagctcat
    11761 tacaccaata atcaaagcat ctaagatcgt actgctgata taggcagata atgtttcgtt
    11821 taattctttt accgtttggc gtaactcttt acgaatcgct ttcggaaaga agtgagacaa
    11881 cgcatcaata aatttgtgcc catccttgaa cataaatagc aaaataaatg gaacggtaaa
    11941 taacaaaata aaaaattgca tcataaatga aatgattttt gagacacttg cagataaact
    12001 gactaatatg atattgaaca aattactaag agataaattt gctgatgcta attgttcttc
    12061 caaattgaaa tttttaaact cttcccgctg cgccaattca tttaaccatt tgcttgtttc
    12121 atctgcatac attggcaagc tctgggtcaa ttgaattgtc tgttccatta actgaggcac
    12181 tacattcatg acagttaaaa caactaaaat gattacgacc aaaaacgtca ataaaaaacc
    12241 gaacactctc gggatccgat gcttttcgag aaaaacaacg attggatcga acatataata
    12301 gataaaacca gcaacaagca atggtaaaaa aactgccgaa agcattttca atattggact
    12361 aaaaatattc ggcatttgta aaataaagta cacgccgatg atcgtgagta ctagctccac
    12421 tgtccagaaa aataattttg attgtttaaa acgtgattgc atacatctcc tccaaggtcc
    12481 tttgatactt ctattctatc atttaacaaa gagttacgcg ctgagatttt catttgctta
    12541 aaaaaaacaa aaaaaataaa catattttaa tgagtagacg agtacttaaa caaaaacacg
    12601 gtttggagta gtgacaaaaa ttatactgca gtcccttttt ccaaataaac ggtgttcaaa
    12661 agcttgcatc ttcgtaataa gcccagccaa acgaaaaata taaggaacta tttttatttg
    12721 gctgggctta ttacttgatg taggacatct attttacaac ctcagatcat ttattgcgca
    12781 atactgacta aatgcaactt tgtgatattt ttgtgtacgg acaactcctc gatcacatcg
    12841 gcataagtga gcccttttgg aagatcaatc gtatagatat ttttgtacac tcggtagtca
    12901 tcaacgaatt ttacgtcaaa attcacatct tcgattacga tatttttctc ttcaaaataa
    12961 tcggtaagaa aatctttggt ttcttttcta tggataaatt ggatttctaa ctttttagtc
    13021 gtaggtacat ggatcacgcg tttcactact gtgagtgcaa tagcaatccc gataaaactg
    13081 atgatcgcaa tggaatagta acccatgccg atggcgatcc ctaatccagc aattgcccat
    13141 aaagaagcgg cagtcgtaag tcccgtcacc gattgtttcg tgacgatgat cgttcccgcg
    13201 cctaagaaac cgacaccact caccacttga gcaatcagcc ttgcttcatc tgaacgaatc
    13261 gttccagcaa gctgtggata ctctaaagca tctcttaatg caccactagc gatctcaact
    13321 tggatcaatg caatgataca tgctcccata caaactaaaa tatgcgtacg catacctgct
    13381 ggacgatttt tatattgtcg ttcaaaaccg attgctccgc caaatagcat agcgatccct
    13441 aaacgtaaaa taatctctgg aattgtcaat accatatcat tcatctaaac accctccagt
    13501 ccaatatatc atgtctgaaa ggagcagaca aaaaaagtgc aatttttacc tcttacgaaa
    13561 aaaggactgt gacaaaattt ttgtcatagt cctttttatg aataaacggt gttcaatcag
    13621 ctgaccctcg gtattactca acaaacggca aaatatgggg agctattttt cctttgtctt
    13681 ctctaatact caggtcaaaa cgctgatttc acaacctctt tagcctacta ttgaaaatta
    13741 caacttactt gctgcttctt catcgatgat cacaacaaca ttgtcgtgat tttgcaatac
    13801 acttgctggc aaatgattcg ttacaggtcc ttcaatcatc cctttgattg catcagcttt
    13861 gctttcgcca aatgccatca agacgatttc tttacttttc ataattgaac cgattcccat
    13921 tgaaacagct tttgttggta catcttctgc tttatcgaag aatcgtttgt ttgcattgat
    13981 tgttgattct gttaagtcaa ctactgaagt cgttccgtca aatgaagcac caggttcatt
    14041 gaaaccgata tgtccatttt gcccgattcc taagatttgg atatcgattg gatgtgcatc
    14101 gatgatttga tcatactttt tgcatgctgc ttccagatct tctgcttttc catctggtac
    14161 aaacgtttct ttgaatggtt ttttatcaaa taattggaca tccatgaaat gacggtagct
    14221 ttgttcatct gaaccgccta gtccaacgta ctcatctaag tttacagaag tcatttcact
    14281 gaaatccaaa tcgcttgaag tcatttcttt atataaagta actggtgtgc ttcctgttgc
    14341 caatcctaat acttttgcac ctttgttcat accttctttg atgatttcaa atgctttttt
    14401 tccgccttct tcagcgtttt ttacacgaat gatttccata gtgattgctc ctttatattt
    14461 tggtatagcc caattatagc attctttttt tctttttaca ataattagta tagaccaaat
    14521 tgcatttttt tcattttctt gacaataaat aggaatccgt gttaggtttc ataagatggt
    14581 tcaatcaaac aatccatgtt gcatgaaaac caagcaagtg attcactgtg gacgctttat
    14641 gtctgcagtt tttttatgaa aaaggagtta ttatgagtat actgacatta aacaacatta
    14701 cccagcaatt tggcgacaaa atcctctatg aaggtgtcga tctgcaatta aatgctggcg
    14761 aacatcttgg gctgattgga caaaacggag ccggtaaaag tacattgatt aaaatcatca
    14821 ctggtgagat cttaccagat gacggacaga tccattggca aaaaaatatt cacaaaggtt
    14881 acctcgatca atatgtggaa gtcgatgaaa cattaacaat cgaagaattt ctaaaaactg
    14941 cgtacgcaaa agaatacgaa aaggaagaaa aaattgcttt tctttaccaa acatatagcg
    15001 agaccttcga tgattccttg ctggaaaaag ctgggaaact ccaaacggaa ttagaccaag
    15061 gagtgtttta tcaaatcgac accttagttg ctgagatgag cagcgggttg gggatcgatg
    15121 tcttaggttt agattcactg ttgaaaaatc taagtggtgg acaacgttca aaggtgattt
    15181 tagcaaaact acttttagaa aacccagatg ttctgatctt agatgaacca accaatcact
    15241 tagatgatca gcatatccaa tggctgacgc agtttcttca ggattttgac ggtgcgtata
    15301 tcgtgatttc ccatgaccaa gaatttcttg atcagatcac cacccatatc gcagacatcg
    15361 agtttggaaa gattactaag tatactggcc atctaaaaca agcattgaaa caaaaggaac
    15421 aaaatcgcga aagctatttg cgccaatatc atgctcaaca aaaacaaatc gaaaagaccg
    15481 aagcttatat tcgaaaatac aaagccggtt cccgatcaac gatggcaaaa agccgacaaa
    15541 aacaattaga taaaatcgaa cggctgacgc ctccagcttc agcaagtaaa gctgtctttg
    15601 attttccatt ctcgcctatt gtcacgacat tagcagtgga aacaactgat ttagtcatcg
    15661 gatatgatcg accgctactt gcacccatca atttgaccat ccgctttggt gaaaatattg
    15721 ccattcgagg cttcaacggg attggaaaat ctactttatt aaaaacactg atcggtgaga
    15781 ttgaaaaaat aagtggcgat ttccacttcc cagataacac aaaaatcaat tacttttcac
    15841 aagatctgta ctgggaaaat ccattagaaa caccactaca atatttaagc agtcaatttc
    15901 caaaagcgac tatcaaagaa ttacgtcgcc aactagctaa agcagggtta gttaatcaat
    15961 tagcaagtga acctctcact acattaagtg gtggggaaca aactaaggtg aaacttgcac
    16021 agttgacaat gaaccaaggt aatttactga ttctcgatga accgacgaac catatcgatc
    16081 aagaaacgaa agaaagctta caagacggga tacagaaatt tccaggaaca gtaatcatcg
    16141 tctctcatga acaagaattt tatcaagatc ttgtgcaacg agtcattgag attgaaccaa
    16201 aataaacgtt tgattgaata gttgaaaaaa aggagctgtg acaaaagttt tgtcacaact
    16261 ccttcttctg aataaatggt ggccaattag ctgaccttcg gtattagctc aaaaaacggc
    16321 aaaatatgag gagctatttt tatttggcta ttcttattac ttgatgcaga acagcttttc
    16381 acaacctctt ttcgtctatt ttttattttc gagatttctc tttcctttaa aaacaactgt
    16441 ttgaaagaag aacgcacatc cttgaaaaac aagcccgcta accgttacac ctaaccagcc
    16501 atactgctgc cacattgtcg tccctagcaa tgaaccagcc gcacctccaa taaagtacat
    16561 aaacataaat acagtattat ttcggttgct tgctgcttca cctagccctt gtactcgtgt
    16621 ttgattggcg acttgaccaa attgattccc gacgtctaat aaaataatag ctaaaagcaa
    16681 aatggccaaa tggcttccgc caaccaataa agcaataaag cttattgttt gcataacaag
    16741 tcccatatac acgatttttc tttcagaata agaatctgcc agtcgtccca cgataggtgc
    16801 agcaactgct ccagacaaac caaaaatcgc taatatccct gcttcgaatg ttccccaatt
    16861 gtaggcggga ctgctgatat aaaaaatcaa cgtagaccaa aagatcgaaa atgttccaaa
    16921 catgaaaaat ccactgactg ccgcttctct taatagacgt tgagaagcaa ctaaatgcgg
    16981 caaactttta agtgaatcaa ggtatgaaac agctgtacta ctttgctgga cagaacgcgg
    17041 cattttccag taaagcagaa gtgtaagaat tccgactgca cataaagcaa ataaatagat
    17101 catccgccaa gaaaaaagac tggcaacaat accagatacc gtccgagaaa gcaaaatccc
    17161 cacgagtaaa ccgctaagca atgtacccat gaccttccct cttgcttttg gtcccgctag
    17221 taccgcaccg taaggaatga tgatttgcgg cacgatcgaa agtaatccta ttaaaaaaga
    17281 agcgcatgca aataacgtaa acgaaggcgc aaaaaaagcg gccaacagag agacggcaga
    17341 taacgctgcc attcgaatga tcaattttgg acgatccacc acgtccccta aaggaactaa
    17401 aaacagtaac cctaacgcat agcctaattg ggtcaacatc gtcaatatcc cgactgcact
    17461 tgtgctaaca gataaactag ctgctacttt tgttccaatc ggctggatgt aatacatatt
    17521 ggcaacaaca acgccacaag tgattgataa tagtaaaata agcgaagttg ttaaaggttt
    17581 tttttcatta ctcttcattg atttccccca tttcctatct tagtttacaa cttatggaaa
    17641 agattggaaa tagaaaaata caactattcg ccacacttat tatttgtgct tttgttttcc
    17701 ttgttttttc ttgcgatctt tataaatgaa gacaactaac cctactagca cagtaacact
    17761 tgcaaccaac gtgactgcag ttttgttata tccgtgaaac agagcttctc caccacacgc
    17821 atacaacaca gacgaaggga tgactccaat cacgatcgac aatatgattt gctgtagagg
    17881 caatttcatc gcatctgcag caaaattgac catagaagat ggaataaccg gtatcatata
    17941 gcctaacatt acacctattt ttggatgttt catttgtcgg attgcttgca cccaatgatt
    18001 cgtttcggtt ttcttatcta aaaattttaa gcgatgcata aggaaaattg cagtgatgtt
    18061 accaagagca ttacccaaaa tgttgatgat cgcacctgtc agtggtccaa agctcaaacc
    18121 agcaatgact ccaactactg aagtaggaat acctggaaca gcacacatca ccgctgtcaa
    18181 tacgatcaag agtatcgatg caaaagtccc atgcgaacga atatgtgcga gtagctctgc
    18241 cttactcgct ctcggattta aaaataattg gatgtctgtc tgatattcta aatacaaccg
    18301 atagcccact accagtatca gaatgattcc caacgagatc aatatcaatc ggattatttt
    18361 ctttttcata tttcctcctg aaaaaaatct atattcatta aataatagtt taacataaaa
    18421 acaaaaaagc accgaaatcc aacataggat ctcagtgctt ttaaatagta aaaattattt
    18481 aacagttaca gatgcgccaa cttcttcaag tttagctttc aattcttcag cttcttcttt
    18541 agatacgcct tctttaactg gtgcaggagc gccatcaact acagctttag cttctttcaa
    18601 gcctaaacca gttgcttcac gaactgcttt gataacttta actttttggt ctccagctgc
    18661 agttaattct acagtgaatt ctgtttgttc ttcagcagca gcagcgccac cagcagcagc
    18721 tacagctaca ggagcagcag cagatacgcc aaattcttct tcaatagctt taactaggtc
    18781 gttaagttct aggatagttg cttcttttaa ctcagcaacg atgttttcaa tgttcaatgc
    18841 cattataatt tcctccgttt aattgttctt ttttatttaa tgctttatgc ggcaattaag
    18901 ctgcatctcc atcttctttg tctgccactg ctttgacagc gtaagccacg ttgcggactg
    18961 gcgcttgtag tacagatagc aacatagata gaagtccttc gcgatttggc aattttgcca
    19021 atgctgtgat ttcttccaat gaagagactt taccttcgat gataccgcct ttgatttcta
    19081 atgcttttgc ttctttagca aattcgtcca tgattttcgc tggtgcgact acatcttcgt
    19141 tactgaatgc aacagctgta ggtccagtga atacttcgtc catgccttca aggccagctt
    19201 ttttagctgc acgtgaaaga atagaatttt tgataacttt catttctacg ttagcatcac
    19261 gaagttgttt acgtaaacgt gttacttcat caactgttaa tccgcggtag tcaacgacta
    19321 ctactgatgc agctgctgta aatttttcgc ttacttcatc aacgattgct gctttttttg
    19381 cgataattgc ttcactcatt tttgagtttc acctcctgaa ttttgatgga agagtttttt
    19441 gcactgaaaa ggcaagtcac ttttatagtt actttgctca accagtatta ttcatcacct
    19501 attcacttcg ttcatagtga ttctactcgc tttgctcgaa aggaaactcc tttcgctttg
    19561 ctcaaccagt attgttcatc atctcttcac ttcgttcata gtgattcaca ataaaaaact
    19621 ctatgccacc gcagacatag agggactatt gaatatattc aataaacgtc ctcggtagga
    19681 aattaaggct tgatgccacc tactgtcttc ggtacaagtt aattattaac ctcaactagc
    19741 ttagcatgct aaactagttg agtcaagaat tatttttcag ttggatcaat caacattcta
    19801 atatttaatt agaaagaagc ttggtcaaca tggataccag gtccaaatgt tgttgtgatc
    19861 gttacatttt tcatgtattg acctttagct gctgatggtt tagcttttag caatacatca
    19921 tggattgctg caaagttttc aactaatttt tcatcgtcaa atgaaacttt tccgattggt
    19981 acatggatat ttccagcttt gtccacacgg taagttactt taccagcttt cacttcgttt
    20041 actgcttttg ttacgtccat tgtaactgta ccagttttag ggtttggcat taagcctttt
    20101 ggtcctaata ctcgtcctag acgacctaca gtagccatca tatcaggtgt tgcaacaact
    20161 acatcaaagt caaaccatcc gttttggatt ttttgaacca tgtcgtcgtc acctacgaaa
    20221 tctgcaccag ctgcttcagc ttcttttgct ttgtctcctt tagcaaaaac taaaacagtt
    20281 tgtgttttac cagttccgtt tggtaataca acagcaccac ggatttgttg gtctgctttt
    20341 ttagggtcta cgttcaaacg gtatgcaact tcaactgttg catcaaattt tgcaatgttt
    20401 gtttcttttg ctaaagctac tgcttcggca acaggataaa ctttacttga atcaactttt
    20461 tttaatgcat cttgcatttt tttgctcttt ttagccattt tggttcctcc ttgattgtgg
    20521 ttataacggt tgtacctccc acgtgttcta tatctttcaa tatagagcca actgagaagc
    20581 tgctgtctta gggtgtatag attattctac agtgatcccc atgcttcgtg cagttccttc
    20641 aaccatgcgc attgctgctt caacgttagc tgcgtttagg tcttccattt ttaattcagc
    20701 gatttctctt acttgatcgc tagaaacttt cgcaacttta tttttgtttg gttcgcctga
    20761 acctttttcg attttagctg cttttttcaa tagaacagca gctggcggtg tttttgtaac
    20821 gaatgtgaat gaacggtctt cgtatacaga aataacaacc ggaatgatca aacctgcttg
    20881 atcagctgta cgagcgttga attcttttgt gaatcccatg atattgatac ccgcttgacc
    20941 tagtgcagga cctactggtg gagctggagt tgctttacct gcaggaattt gcaatttaac
    21001 gattttttct actttctttg ccacgagaca tacctcctta agtccgtgat gtggttaatt
    21061 ggagatgcat atttctcctc ccacgtttta tctgatctga aaaaatccag acaaataatg
    21121 tgcaaaacaa tgcacactgg tacagtatag caaatttatt tgttttttca agattttttt
    21181 ctgctatttt tacattttgc ttgaataaac gctaaattta ttctaaaaaa acaacaattt
    21241 ttcgttctca tttcttcttt tatgttacac ttgcgctagc atggttcaac agatgaaagc
    21301 aaacaataac acggtttttt agacccttgt acaaagattc ctacataaca aaatgaggtg
    21361 gagaaaatga aagatttaag gtatagaagt gtctttgata ttattggacc tgtgatgatt
    21421 ggtccaagta gctctcacac tgccggagct gctagaatcg ggaaaatcat acgtagtata
    21481 tttggcgaac agcctgattc tgtagatatt tatctatatg aatcatttgc aaaaacctat
    21541 cgggggcacg gaacagatat tgcattagtt ggcggcctgt tagggatgga acccgatgat
    21601 gaacgtttag ctgattcttt gaaattggca tatgaagccg gtatggaagt cttgtttgtt
    21661 cctaagagtg aaaaagccga acatccaaac tctgtaaaga tgcttctaaa aaaaggaaca
    21721 cgaaagcttt cagtcactgg catttcgatc ggcggtggca atatccagat ttctgaattg
    21781 aatggattca aaatttcttt aagcatgggt actcctacct tgatcgttgt ccaccaagat
    21841 gttccaggta tgattgctaa agtgactagt atactttctg agaccaatac gaatatcggc
    21901 actatgacag tgacacggga atcaaaaggt gaaaatgcca ttatgataat tgaagtagat
    21961 gatcctcaag tagaagaaac ggtaaaaaga ctcaaacagc tgccaaatat cgacagcgta
    22021 aattatttcg attaaggagc taaaaaaatg tttttatcga ttgaagaatt agtagatcaa
    22081 gcaaaagctt atccaagtgt agcagaattg atgattgcag ttgaaatgga aatgagtggc
    22141 cgttcccgta gtcacatcat cgagatcatg gaaaaaaacc tctcagtaat ggaacaatca
    22201 atcaacgagg gaaaagaagg tgttacctcc gtcacaggaa ttaccggtgg tgatgcaaaa
    22261 aaattagatg attatttaaa tgaggcagac tttttaagtg gagaaacaat cttaaaagct
    22321 gtacgcaatg ctgtagctgt taatgaagtc aatgcaaaaa tggggttgat ttgtgctaca
    22381 ccgactgccg gaagcgcggg tgttgttcct ggtgtgctga tggctgtccg agatcggctt
    22441 gatttgacac atgaacagca attggaattc ttatttaccg ctggcgcttt cgggttagtc
    22501 atcgcaaaca acgcttctat cagtggtgca gaagggggat gtcaagcaga agttggttct
    22561 gcaagtgcaa tggcagcagc agctttggtt tgtgcaaaaa atgggactcc tcatcaagca
    22621 gcacaagcag tagccattac attgaaaaat atgatgggac tgatttgcga tcccgttgca
    22681 ggcttagttg aagtcccgtg tgtcaaacgt aatgctttag gctcttctca agcttttatc
    22741 tctgctgata tggcattagc gggtatcgaa agtgtcattc cgccggatga agtcgttgct
    22801 gcaatgtacc aagtcggacg tcaaatgccg tcgattttca aagaaacagc agaaggtggc
    22861 ttagctgtta cgccaactgc taaacgactt gcggaagaga ttttaaataa gagggcgtga
    22921 taaagctcct taatttcgag tattaagaga ttagttatct tcataacaaa aaacgaagag
    22981 aggactaatt gcccttctct tcgtttttta ttaatccatc aatttcgtat tttttacttc
    23041 atcgatcgtt tttgttcctg caagttgcat cgtgatcgac aattctttgt tcaggtgttc
    23101 aaaaacagat gttactcctt ctgccccacc tagattcaac ccataaatca ctggtcggcc
    23161 aatcgcaact aagtcagcac cacttgccag agctttgaag acatgctctc ctcgacggac
    23221 accagagtcg aaaatcactg gtacacgttt gttgactact tcggcaattt ttggcaatac
    23281 ttcaaaggac gcaggacctc catctagttg acgtccacca tgattggata cccagatacc
    23341 atctgcccct gcagagatag cgattactgc atcttctggc gattgtattc cttttacgat
    23401 aactggaaga tgggtgattt ctttgattgt tttgatgtca tctggtgtca agccttgttt
    23461 tgcagctgca taaatttctg cgattccttt tccttcgcca ttaccacttg cactttgttc
    23521 gctatacgca gctaagttag gcattggtaa tggaaattgg aattgattga tgacgtcttc
    23581 ttcacggtaa ccaccgagcg ttgaatctgc cgttaaaatg atagctttag cccctgcttc
    23641 tactgctttg ttcaaaatga actcattgaa tctgtcgtct ttactcatat ataattggaa
    23701 aaactgcggt gcgcctggcg ctgcatcggc tgcgtctttg attgtcgtat ttgcataagt
    23761 gctgatggaa aagattgatc cagcagcagc tactccttta gctgtatcag cttccccttt
    23821 ttcatgtgcc aatccttggg ccgctgatgg cgcttggata atcggtgtat ctaattcaat
    23881 tccgaaaaca gatgtatgca aatcggctga gtcgattccg cgtaatatcc gtggcatgat
    23941 tttctttgta ttaaaagaag tcgtattttc tttcatcgtc cactcatctt cagaaccacc
    24001 gcgaatatag ccaaaagctc ctgcttccat gcgctctttt acacgacctt ctagttaggc
    24061 aatgtttaca atatcgattg gatgttcttc attacttgta tgataagtca tagtagatcc
    24121 ttctttcatc ttcttgatta catataaatc atactaaact tacaaaaagt aagcaaacat
    24181 ttactctcag atacttttat cgctattctt tccaaataaa tcgacttaca agctgatcta
    24241 cttcttggtt ttcatgaagc atactatgag aggcattttc acctgtcact actttttcct
    24301 ctatttcatt tccgcgttgc ttcaacaacg catagaccgc taatgcactg tttaacggta
    24361 ctgttccatc actcaagtct gtttcatcta gttgaccagc aatcaagaaa aatcgagtgg
    24421 ttataggaat attaccgatc atttgctggt aatcttgata gcggttagaa acgacagcag
    24481 gcccctttgc taactcatct gtcagagact gcgcactatc atcgacaaaa tcattaaaag
    24541 gcgcgccaat tgctgttaat gtctgaactt gtggcaatga cgtatcctgt ccatacgttc
    24601 ctaaatagcg tagtgcacta actcctccca tggaatgccc cactatgttg actttatcta
    24661 tgccgtagtt ttctttcaga tacaaaagac aagttttgat ccattctgct tgattccact
    24721 cgttattttt attatcttca aacaatactt ggaccatcgg attgtctgct tttccagaca
    24781 aatctcctgt tgcttgtatt tgtccatctg ttcctactgt taaaaccaat tcttttactg
    24841 cttgctgatt tttttccaaa cgccgcaaca tccctttaaa ggaattttct gtcccgctgt
    24901 agccgtggaa aaaaagtgtt ggaatagtgg ttttttctat cactttactg gattctgccg
    24961 ttttttcttt atcagaaact tcagtagtat ttatcttcgt cgtctggcaa ccacttaacg
    25021 caaacaaact gataaggact aaagctgctg cagttgcacg tttgcataaa tttttgattg
    25081 gttctgtcat acacctcttc ctttccatat agaaaaaaga aagacttgaa aggccaatca
    25141 tatgatcgac atttcaagtc tctttattaa tcgattttat ctacttgatc gaaatcaagt
    25201 tctgtactgg tttcacgtcc aaacatatcg atgttgactt tcaatttttc tttttctccg
    25261 tcaacttctg ttacttgtcc ttctaatcca gcaaatgcgc cttcgatgat tttcacgact
    25321 tcgccaactt ctacttctag atcattttgt cgcaggctca taccgataga acgcaagata
    25381 tgattgactt cttctggaag taatggtgct ggtttgcttc cggctccgtg tgaaccaacg
    25441 aatcctgtca caccaggtgt gttgcggaca acataccatg aagcgtctgt catgatcatt
    25501 tcaacaagta cgtatcctgg gaatgtttta tgaacgattt ctttttcttt cccatttttg
    25561 acttcagttt ctttttcttc aggaacaacg acacggaaaa taaagtcggc cattttcata
    25621 ctttgcgcac gagattcgat atttgctttt actttgtttt catatcctga atatgtatgc
    25681 aagacatacc agtttttttc aaaagattcc attgaaggct cctcatttca ttgtaaaata
    25741 aaaaaacctc agtacacccg aagtttttct ttcgcttcat tatagcacta aaagtacgtt
    25801 gtggctagcg agttatttta aaatccagcc aaataagctt tggatggctg tatccattac
    25861 gtaaaacatc aatccgaaaa taatagaagt ttcgatgact actaacgtat ctttacggag
    25921 ttgttttctg cttggccaag ttacttgttt gatttcatct tttacgcttt ttagaaattt
    25981 catgagacaa cctcccttgc tactgtttat ttcgtttctt tatgtaatgt atattgatta
    26041 caatatttac aaaacttatt gatttccaaa cgttcgccgc tttttccttc actgactgat
    26101 tttgtgtagt tgcgagaccc acaaacggaa caagcgagtg ctgctttttt ctttgccatt
    26161 tacttctgcc cccttgctga gaatcagact tatacttcat aacagtatca cgtaactttt
    26221 tacttgtcaa tttgtgaaat aaactatcta ttgttttgga taaaattatg atatgattaa
    26281 tttgaaatta agctaaaatg agacaattgg aggtatagcg atgctattaa aaacacttgt
    26341 atataagaaa aaagatctga caaccgttac agagactagt acattagaag aagcattaaa
    26401 aattttagaa acttccggat accgttgtgt gccgatctta gattcttcag gtaatatttt
    26461 ccgtggaaac atttacaaaa tgcatatcta tcgtcataaa gcaaatggcg gtgatatgac
    26521 gcttcctgtc actcatcttt tgaaaaacgc tacaaagttc atttacctgg atacttcttt
    26581 cttcaaagta ttctttacga tcaaagagct tccttatatt gctgtccttg acagtgaaaa
    26641 tcatttttat ggtattttga cacatagtac gttgcttaat atgctctctc aatcttggag
    26701 cgtagaacaa ggaagctatg tactgacaat tgcttccaca ggaaagcaag gagatttggc
    26761 tgctattgca aaaatcattt caaaacacag cagcattgcc agctgtatta ctcttgatat
    26821 cgaaaaagac gaatacattc gacgtacatt gatcacgcta ccggctaaca ctgaagcgaa
    26881 agtatgtgaa acgatcgtca accacttaga gaaaaagaat tataaagtgg tagaaattga
    26941 aaatttgcaa aaccaagaag cataatcaaa aagcgaaaaa agaaaacagc taatctgctt
    27001 tcttttttcg ctttttataa ttagacacgc ttttcaaaat caagcgcgcg atctaaataa
    27061 tacatcaatg gtgctgaaac agccatgacg ataaactcac ttaatgcaag cgtgccatag
    27121 gttgtccaaa atgcttggcc acctgttgga actaacatat aagcaatcag tcccatgctg
    27181 atggtaaaga agatcgtgtt caatactaaa cgcatcttta catttttcac tttgttttgc
    27241 aataaagctg tcaggcctaa agaaagcaat gtttggccgc caccaaaaac cacatccatg
    27301 ataccaaaac caaagaaggc attatagacg atgaccccgc ctaaaactcc ccaaagcaat
    27361 ttacggttaa agacaactaa atgattcaaa ctttctgaaa tacgaaactg gatcggccct
    27421 gaggatacag gcgcaactaa aatagttaac gctagataaa gagccatgat gatgccattt
    27481 acaacgatta cggataaccg ttgtctgctt ttcgtttttt gcattttctc tcctcttttc
    27541 tcctagtttt tttaacgcgg gatggttgat gaaccgcggt tatgtgaaac aaacaataat
    27601 tataacagac ttttcattta tttcaatccg tcttatctgc ttttaaaaaa gcaaaagctt
    27661 aaacaatgta aacgtttgct aaaaatcgtg ggatatggta taaagtaagt gtaaggggtc
    27721 ataagaattc ccagtaaagc agacgaagta ggatctgcaa gaacagaatt gttgtgaaac
    27781 ttaaataaat aaaaaaggat gtgggaattt gagcaacctc ttactagaga acggtctagc
    27841 aagcagctga agatctaagt ggacatgccg aaccttggaa cttctcctta cgtaagtaat
    27901 ggatcatgcg cttgctaggc ttttttgtgt ttttcggtct tcggaccttt ttcattttcc
    27961 tagacttctg ctataataac caagaattaa ttttgtaaca gatttttttg aaaaaacaag
    28021 tttctttcat tcaaaaaata gaaaaagctt gctaattatt ggttaaccag caaacaggag
    28081 tattaaaata tcttgtttta ttgaatagca ggaggaaatc atgaaattcc attatcatat
    28141 attgatcaat ccatcggcag gcagcggaaa tggctataaa gtagcagaaa gaattttacc
    28201 ggtattaaaa aataagcata ttgactatac acttcattat agtgaataca aaggacacga
    28261 tgctgagatt gctgaaactt tggcaaaaga aacattgatt ccttggatag aagaagaaca
    28321 gaaaacaatc gatactttcc cccttttgat catcgtagga ggtgacggca ccttacatca
    28381 ggtacttgat actttctatc agatggaagt agaattccca gttgcttata ttccagcagg
    28441 ttctggagat gattttgctc gaggggcagg ccttccaaaa aatcccaaaa aaggacttca
    28501 gctgatttta gcagctcaat caccagaaaa agtccacata atggcttatg aagaaaaaat
    28561 aagtgaaaaa aaaggaatag ctgttaataa tttcggtata ggattagatg cagctatcgt
    28621 ccatgcgaca aatcattctt ccacaaaaaa gcgattgaac aaatacaact taggatcatt
    28681 ttcgtattta ttctctattc tccgtgcttt attcactcaa aaaggatttc caattctggt
    28741 agatgcagga ggaaaacaat taagtttttc aaacgcattt ttatgtacag caacgaagca
    28801 tccttttttt ggcggaggaa tcgcgattgt tccaactgct gatgcgtcta aaccagtcat
    28861 tgattttgtc gtggtcgaac gaatcaattt ctttaaaatc ttgtggctca ttcttttact
    28921 tatacagaaa aaacaattga aatcgaaata tttccatcac tacacaacaa acagattacg
    28981 tatcgtttca acaacaccgc aacatggtca agaagatgga gaagaaatgg aaaaacgacc
    29041 ttttgatatc acaataacca caactaaaca gttgttttgg tgttaataaa agaggttttg
    29101 tcagaagcta catctggaac accgtttatt cggaaaaaag gactgtgaca cgacttttgt
    29161 cacagtcctt tttttactat gcagttaatt ctttgcccaa ttgggttgcc atcgatagtt
    29221 tctttcttta cccaaaataa attggatcat tgaaaacacc gcagcaaaag caactaagac
    29281 acaaccattg aaccaaaaag attctgcaaa tgtttgattg taactgagtg tttcactgat
    29341 caagttatta ttttgaacaa accaatatgt tggtgtgaat gccgctattt tgctaaccat
    29401 ttctggcaag tattctgctg gtacaaacac cccaccgata aagcaactgc ctgtgataaa
    29461 aatatctttg atcccaccgc tggtttctga gttttttacc aatgaagtga tacaagccga
    29521 aaagctaatg atcggcaata aaaacaagaa agtatttaaa atatgatacc ataccgttat
    29581 atcccacgag gtgtggatca ctaacagaac cattagaagt aagaagccac tgaacaacag
    29641 cagcgagtaa ctaatcaaac taaaggttac tttgcgattc aagtttcgtt tggtaatggg
    29701 cgcactctgg tttcgtttac tgatctcttt gcgattaaat gctagattga tgacgctgaa
    29761 accgctaaaa atggatagaa acaacccata agacaagaaa ttaaaggttg tgcccgtcaa
    29821 cagctttttc atccgctgac tatatcccgc acttagctca acttctccag taaccgagag
    29881 tgccatttgt gtctgttcca tagctgcatc gattgtttta tcttcttctt gaaacagaat
    29941 cagtgtttgc aaaaaggtgt tcacttgctg tgtaacaact gttttagaaa aggcatctgg
    30001 gcgactctgt atcttgatcg tcggcatctt tcctacttcg acttgttcgg taaagtctgc
    30061 aggcagatgt aagataatgt ttacacgatt aaaataaaga gcatcatcga tttctttttg
    30121 tgaggttcca tccaatgtaa cgactgtttc cttgtcattc aaatatttac gaaggccatc
    30181 gactacaggc gactcttctt cactaatcaa ggcgattttc gcatcttgca gagaggtatt
    30241 ttcaacatca atattttgcg caaaaaagaa tgtggcaaca acagtgataa ttacacctat
    30301 cagtaaattc gatttatttg ctttaagcaa tgtccaaatc gttttataaa gcgtcataac
    30361 gcacccctct ttccatccaa gcatttccta acgcaaaaag gaaaataaat ccgatcaacc
    30421 atagaagatt ggtataaaaa ctagccaaat cagtgtagaa ataaagttt
//
LOCUS       DARBFO010000025        29254 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000025 DARBFO010000000
VERSION     DARBFO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.026"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(10..858)
                     /locus_tag="SCK51_001254"
     CDS             complement(10..858)
                     /locus_tag="SCK51_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1251892.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1082..3022)
                     /gene="thrS"
                     /locus_tag="SCK51_001255"
     CDS             complement(1082..3022)
                     /gene="thrS"
                     /locus_tag="SCK51_001255"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEG1251893.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3359..3538)
                     /locus_tag="SCK51_001256"
     CDS             complement(3359..3538)
                     /locus_tag="SCK51_001256"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEG1251894.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3585..5408)
                     /gene="cadA"
                     /locus_tag="SCK51_001257"
     CDS             complement(3585..5408)
                     /gene="cadA"
                     /locus_tag="SCK51_001257"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEG1251895.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5686..7965
                     /locus_tag="SCK51_001258"
     CDS             5686..7965
                     /locus_tag="SCK51_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1251896.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8058..8705)
                     /locus_tag="SCK51_001259"
     CDS             complement(8058..8705)
                     /locus_tag="SCK51_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251897.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8884..9603)
                     /locus_tag="SCK51_001260"
     CDS             complement(8884..9603)
                     /locus_tag="SCK51_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1251898.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9734..10885)
                     /locus_tag="SCK51_001261"
     CDS             complement(9734..10885)
                     /locus_tag="SCK51_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEG1251899.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11102..11842)
                     /locus_tag="SCK51_001262"
     CDS             complement(11102..11842)
                     /locus_tag="SCK51_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1251900.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11846..12169)
                     /gene="rsfS"
                     /locus_tag="SCK51_001263"
     CDS             complement(11846..12169)
                     /gene="rsfS"
                     /locus_tag="SCK51_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEG1251901.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12226..12819)
                     /gene="yqeK"
                     /locus_tag="SCK51_001264"
     CDS             complement(12226..12819)
                     /gene="yqeK"
                     /locus_tag="SCK51_001264"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEG1251902.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12803..13453)
                     /locus_tag="SCK51_001265"
     CDS             complement(12803..13453)
                     /locus_tag="SCK51_001265"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEG1251903.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13463..13774)
                     /gene="yhbY"
                     /locus_tag="SCK51_001266"
     CDS             complement(13463..13774)
                     /gene="yhbY"
                     /locus_tag="SCK51_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEG1251904.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13790..14899)
                     /gene="yqeH"
                     /locus_tag="SCK51_001267"
     CDS             complement(13790..14899)
                     /gene="yqeH"
                     /locus_tag="SCK51_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEG1251905.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14896..15423)
                     /locus_tag="SCK51_001268"
     CDS             complement(14896..15423)
                     /locus_tag="SCK51_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEG1251906.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15603..16388)
                     /locus_tag="SCK51_001269"
     CDS             complement(15603..16388)
                     /locus_tag="SCK51_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEG1251907.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16381..17250)
                     /locus_tag="SCK51_001270"
     CDS             complement(16381..17250)
                     /locus_tag="SCK51_001270"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEG1251908.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17251..18630)
                     /locus_tag="SCK51_001271"
     CDS             complement(17251..18630)
                     /locus_tag="SCK51_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEG1251909.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18638..19057)
                     /gene="fabZ"
                     /locus_tag="SCK51_001272"
     CDS             complement(18638..19057)
                     /gene="fabZ"
                     /locus_tag="SCK51_001272"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEG1251910.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19082..19558)
                     /gene="accB"
                     /locus_tag="SCK51_001273"
     CDS             complement(19082..19558)
                     /gene="accB"
                     /locus_tag="SCK51_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEG1251911.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19561..20796)
                     /gene="fabF"
                     /locus_tag="SCK51_001274"
     CDS             complement(19561..20796)
                     /gene="fabF"
                     /locus_tag="SCK51_001274"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEG1251912.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20815..21552)
                     /gene="fabG"
                     /locus_tag="SCK51_001275"
     CDS             complement(20815..21552)
                     /gene="fabG"
                     /locus_tag="SCK51_001275"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEG1251913.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21564..22481)
                     /gene="fabD"
                     /locus_tag="SCK51_001276"
     CDS             complement(21564..22481)
                     /gene="fabD"
                     /locus_tag="SCK51_001276"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEG1251914.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22588..22812)
                     /locus_tag="SCK51_001277"
     CDS             complement(22588..22812)
                     /locus_tag="SCK51_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEG1251915.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22865..23827)
                     /locus_tag="SCK51_001278"
     CDS             complement(22865..23827)
                     /locus_tag="SCK51_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEG1251916.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23837..24277)
                     /locus_tag="SCK51_001279"
     CDS             complement(23837..24277)
                     /locus_tag="SCK51_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1251917.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24583..25548
                     /locus_tag="SCK51_001280"
     CDS             24583..25548
                     /locus_tag="SCK51_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEG1251918.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25605..25835)
                     /locus_tag="SCK51_001281"
     CDS             complement(25605..25835)
                     /locus_tag="SCK51_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEG1251919.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25921..26808)
                     /locus_tag="SCK51_001282"
     CDS             complement(25921..26808)
                     /locus_tag="SCK51_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEG1251920.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26897..28120)
                     /locus_tag="SCK51_001283"
     CDS             complement(26897..28120)
                     /locus_tag="SCK51_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG1251921.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28137..29183)
                     /locus_tag="SCK51_001284"
     CDS             complement(28137..29183)
                     /locus_tag="SCK51_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG1251922.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
BASE COUNT         8098 a         6414 c         5047 g         9695 t
ORIGIN      
        1 gtaaggtttt tatttcgaat ccataaaata ttcttggatt gcttctcgtg tttttttcac
       61 tttttcgcta agcttttcgt ttttagccat atagaaataa aaaatgatat cgatcgtagc
      121 aaattgggcc aaaagtgaat tagttgccgc acttcgattc gtgatttctt ttggtctaga
      181 tgtttgtacg agtacatcgg ctatcttgct aagcgggtta tttccaaatt gcgtcaacgc
      241 aatgatttcg atattcatgc ttttcgccag ctcggctaat gcgactacat ctgcacttct
      301 acctgagttt gaaacaagcc aaagcacttt ctttttctca ttgctgacaa gttgcgggag
      361 caagacgtgg atatcttttt caaaaatgat tggcttaccg acacgagacc atttttgcag
      421 aatatcttct actactaaag aagaggcgcc aaccccatat aaaaaaacag tctctgccga
      481 ttccaaacac tctacgactt tttggatcgt ttcttcttct agaatatcta ctgtttcttt
      541 tatcgctact tgtgcattgt taccaagttt gtttttgata gagtaaaagg actcatttgc
      601 ttctatatca ctaaaacctt catcgattcc agtctgtagt tctgttgaaa tcgtgatttt
      661 gaagtccgtc agactttgaa aaccgatttt tttggaaaaa cgtacaactg tcggcgcact
      721 caacccggaa gcttctgcta attcattagc tgtcattgta gggaccttct caatattacc
      781 gataatgtag ttggccaact ttttttctgc tgaagaataa tcttccatca tactttcaat
      841 acgactaatt acgctcataa gttcctccac ccttttaatg atagtctatg taagtatacc
      901 atatcagacc cttattcacg gtcgtatact ggataaaaaa caaaaaaaca atccgcccaa
      961 cagaatatct tatcccatca atcccacaga ttctattgag catctgtgtc tttgaggaat
     1021 acctgttatc tattgttcgg attgctttct atctactact tttttacagg aaatttacct
     1081 attagttttc tctgctgtaa tttttgactt ctgcttccat agcatcaata aagatattca
     1141 aatcttctac agctgtttct ttacttccat aacgacgtac gttcactgtt gcatcttcca
     1201 attccttgtc tccaacaact aattggtaag ggatcttttg tgtttgagag gcacgtatct
     1261 tatagcccat tttttcatta cgatcatcaa cttctacacg caaccccttc atttgcaaac
     1321 gttctttgat ttcataagca taatcgccat gtgcatcaac agaaacaggg atgattgttg
     1381 cttgtatagg tgccaaccat gttggaaaag cgcctttgta tacttctgtt aaataagcga
     1441 caaaacgttc cattgtagaa acaattccgc ggtggataac tactggacga tgcgtatttt
     1501 ccccgtcttc tccaacatat gtcaaatcaa agcgctctgg caataagaag tctaattgta
     1561 tagttgataa tgtttcttct gtaccaagag ctgttttgac ttgaacatct agttttggtc
     1621 cgtaaaaggc tgcctcgcct tctgcttcaa agtaatctag ttctaattca tccatagcgg
     1681 ctttcagcat tgcttgggct ttttcccaca ttgcgtcatc atcaaaatat ttatctgtat
     1741 tgtttggatc gcgatagctc aagcggaaac gataatctgt aatattgaag tcagcatata
     1801 ctgccaccat caattcaagc gtacgtttaa attcatcttt gatttgatca ggacgaacaa
     1861 atgtatgacc atcattcaac gtcatttctc gtacacgttg taaacctgac aaagcacctg
     1921 atttttcata gcgatgcatc attcctaatt cagcgatacg aattggtaat tcacgataag
     1981 aatggatcgt gtttttgtag accatcatgt gatgagggca gttcattggg cgaagaacaa
     2041 gcatttcgcc atctcccata tccattggcg ggaacatgtc ttcttgataa tgatcccagt
     2101 gtccagatgt tttgtacaac tcaacatctc ccattattgg tgtatataca tgttggtaac
     2161 ctagactaac ttctttgtct acgatataac gttcgatcgt gcgtcggatt gttgcccctt
     2221 ttggaagcca gaatggcaaa ccagaaccta cttctgggga aaccatgaac aagtcaagtt
     2281 cttttcctag tttacgatgg tctctttctt ttgcttcttc acggagacgg atatattctt
     2341 tcaaagcttt cttgtcaaaa aaggctgtcc cgtatacacg ttgcatcatt tgattgtttg
     2401 agtttccacg ccaataagca cctgccactg ataataattt aaatacttgt atacggccag
     2461 tggatggaac atgtggtcca cgacacaaat cgatgaattc gccttgttga taagcagtaa
     2521 tcacttcttc ttctggcaat tcactaatca gttctacttt gtacggatca cttgcaaaaa
     2581 tttctaatgc ttcctgtttt gaaagaacac ggcgttcgat cggcagattt tctttgacga
     2641 ttttcatcat ttctgcttcg attgccggca aatcttctgc agttacttgg ttttgtccat
     2701 tatctgtatc gtagtaaaac cctgaatcga tagctggtcc aacgccaaag tggatatttg
     2761 gatagaggcg acgcatggct tgggccatca aatgagctgc tgaatgacgg actaagccaa
     2821 gtgcatcttc atggtctggt gtgatgatct caatacttgc atcttcctcg attggacgag
     2881 tcaaatcaac gagttttccg ttcactttac cagctaaagc tttttttgct aagctttttg
     2941 aaatgctttc agcaattgcc aatgttgttg ttccagactc aaattctttg actgcaccat
     3001 ctggaaaagt aatttttatc ataatagttt cctccttgaa atgttttgat tgatcagatc
     3061 atcaagctga aaaacaaaaa aatcctagca tctcttgaaa agagatacta ggacgaaaag
     3121 ttcgtggttc cacctaatgt tcaaacgcaa atgcgttcct caagcgttgt aacggtccga
     3181 ccgcccttgc ttcaacaagg gtttcgaaag tggtcatcct ttggttttct cctggaaaag
     3241 tttcagccta gcttttcctc tcttatcgaa gactctcaaa ttcagttggc tttctcatca
     3301 atcaattatg tgtttattta accactaata aatagaaaaa tcaagtggga aactaaactt
     3361 attttttcca ttccccattt acaccgtatg ttcctttttt catctcattg atgatgacat
     3421 ggatgtgttc ttttggtgcc tgcgtgtttt tatgaacagc ttccgtgata tctttcatca
     3481 tattttctaa ttgttccgga ctgcgacctt ctactaattc gacatgtacg aatggcataa
     3541 tgctctctcc tctttctgat cgttcttttt tcaactcgat tctatcatac tatgtttgtc
     3601 ttgggtactg aatcaggaaa aatggctgga tctttttttg ctctcaaggc actcaaaata
     3661 gagaccgtat ccacgacttc ttgcaaaaca gctccgaata atgcggggat tagtcccgta
     3721 cttgcaatta acatcaaaaa aatacaaata aagatgccaa tcagtacaga ctgtttcgct
     3781 attctcatcg tatctcttga aatcactact gcttttgcta cacgcaagag atcatctttc
     3841 aagatgaccg catctgcact ttcactagca gctgtagaac catgagcccc cattgcaata
     3901 ccgatatctg cagcagcaag tgctggtgcg tcattcacgc cgtctcctac catcgcaaca
     3961 ggtctttgat ctttagggat actttctaaa atggtcagtt tatcttgagg caagctacgg
     4021 gcatgtacag aatctaattg gactttttct gcaatcgttt gagcaatggc agatccatcc
     4081 ccggtgatca tcatcaaatt tttcacatca agttttctta gctccttgat tgtctttgct
     4141 gcttctgggc gaatctgatc cgaaaatagg atcgttcctg caaaagatcg atcaattgtt
     4201 acatagattg ctgtattgga cgtttctatt tcttcagctg aaacaaaaga ggcacgtcca
     4261 atctttactt catgattatt gactactgct tggattcctt gaccggtcac ttctttcagt
     4321 tgactaacag gatacagttg gctgtttttc acgttgttca ctaacgaacg agctaagata
     4381 tggctagatt cctgttctgc actagctgca tacagaagta gctcttcttt ttttattcca
     4441 ttgactggct gaatcgcctc tacttccaat atccctttgg taagcgttcc tgttttatca
     4501 aaagcaatcg tttttgtacg tgcgagcttt tcaatcgttg ttccagtttt tacaacgatc
     4561 ccgtttttac ttgaacggct cattcctgca actaaagcaa tgggcgcagc tagaatcagt
     4621 ggacaaggtg atgccactac cagtacctca gcaaatcgta aagggtcttt tgagaaccac
     4681 caagaaatac caccgatcaa ataagcaatc aacgtgaaag ggactgcata acgatcagct
     4741 aaacggacaa atcgggcagg cttttctttt gattcttcga ctagtttaat aatcatttga
     4801 tactggctgt ctgcggcacg tttttcagcg atcatttgga tcgatccgtc tccatttaaa
     4861 gaccctgaca taatctgatc tccaggtttc ttggtttccg ggcgggactc acctgtcaaa
     4921 gaagattcat cgaccgaaga ttctcctttg gtcactcgac catctgcggg tacaagttcg
     4981 ccaggcttga ctagtaactg gtcatcaata tttatttcat cgattactat atcctgcaat
     5041 tttccttctt tgaatcgatg ggcaattctt ggggtgttat ctaacaaaga ctgcagttct
     5101 ttacccgctc gtttattcgc gtaatcctct agactatctc cgcccgtaag catgatcaat
     5161 atcatcaaac tagcccagta ttctttgacg gctaatgttg caaggattgc ggtaatcgct
     5221 aatacatcta cgccatatct tcctgaacgt aacgtcttga tcatttccaa aaacatcaag
     5281 aaggcgagaa tccctccagt aaatgcgaca atcgcaaatg caagggccgg ctggtgaaaa
     5341 acaaattcac ttaataaagc tatcccacca ataagcaaag taagtaaaaa ttttttgaaa
     5401 tgtgtcattg ttttttctcc tcttttatgt gacttagaga ggttagtaat aaatttccag
     5461 ctgcctcttt catcttaagc atagtccttg actagcactt tttaaaacaa taggccttaa
     5521 ttgaaaacaa ctatcactat cagcgtttcc aaaattcatc agttctacaa acaaaaatat
     5581 tcagcaagga ttactttttc gattcatcag ttaaaaagta ccttttttcg aaaaactatg
     5641 ctatactttt aattcgttat ttacggcgga aggagcaaac atgcgatgaa tgagaaacaa
     5701 ctagaacaaa cgcacgtgga tgaaacgatc gaactaatcc agatcgaaca aaatatgctg
     5761 gatacaaaac aacaagaatt gactcaggaa atgaatcatt cgacaaaaga cacagctaat
     5821 cacaagattc gagctggttc caatgaagct ttttatgaat ctgctgttga atatcgccaa
     5881 catgagcagg aattgctttt gaaataccat actcttgaag cacagcaaaa acgcctgcaa
     5941 acgttgtctg tcatgaaaga tagtccttat tttgcccgta tcgactttaa agaagaccgt
     6001 gaaacagaaa cattatatct aggtatcgct tctttaagag atctggcaga agaaacaatc
     6061 gttattgact ggcgcgcacc aattgccaat ctttattatg aaggtgagat tggtcccgca
     6121 ttttatgaaa ctgaaacaga tagaatagat gtcgatctgc agttgaaacg acaatttaaa
     6181 atccaagaag gacgtatttt atctatggta gatacgtcgg aagtaatcaa tgatgacttt
     6241 ttgctagaga tattggatga atcatcaagc aaccatatga aaaacatcgt ctctacgata
     6301 caaaaagcac aaaatgcaat cattcgggac acaacaagta aagtaatgct gattgaagga
     6361 atcgccggaa gtggaaagac atccgctttg cttcaacgta ttgcttttct gttgtatcat
     6421 aatcgcaagt ggctcgaggc tgaaaacgtt cttctatttt cacccaacca tttgttttct
     6481 gattacatct cgactgtatt gccttcatta ggtgaaagcg gcgtgcctac acagacattc
     6541 aaaaattatt tggtgcagct gctgccagag ttttctttac tagatgaaca gcaacaagaa
     6601 agtggttttt tgctagggga aaaagatccg atacaagtga tgaaaagcgg attgacctta
     6661 gtcgatcaga ttgatcgcta tatccaaggc atcacaaact acggtccctt gttccgtgat
     6721 atgaaaataa atggccgtac gatcctatca aaggaatcga ttcgccaatg gtataaagaa
     6781 acaaatgaac aattaccttt acatcataga ctttctctat tacaaacgaa gctcctgaaa
     6841 aaattaggcg gtctgcaaaa agacgaaaca cgtcagcaat gggtcaaaga tctggcggaa
     6901 gaacagctcc aagaactata cgcaacagat ccaaatcttg aatatacaga acaaaaggag
     6961 cgttcattac gtaaaaaact ggccaaccaa atcgtacgta aaagattccg tcgaatccat
     7021 cgagggatca tgaattatca atttgtcaat ataacgaaac aatatctcca ctttctacaa
     7081 actgtgccac tttctcttct aacacgacat aaaatcagcg agaccaggtg gcaggaacat
     7141 ttgacaactg ttcgtacaca cttgcgtcaa agagaattgc gtcaagagga tgctgtacta
     7201 ttctttttac tgatgcgccg aacatatcca gtgacggtta cacacaaagc tcgctatatc
     7261 tttattgatg aaatgcagga tttcgcacca tcacaagtgg cattgctgca agccttgttc
     7321 ccaaaagcaa atttgacttt ctgtggagat ctaaatcaaa atgtctttgg aaatgaaacg
     7381 atcgttggat cattggaaga ccttttcgtg gaccgtgaag tcactcgttt ccaattaacg
     7441 acaagctatc gctccacaaa agaaatcact gattttgcta atcattttat tgtgaaagaa
     7501 catcaagtag aaacaacagc aagaaaagga gaccttcctt tgctcatcca aggaacaaca
     7561 gatgatatca aattgaattg gttagcagat accattcgaa cgactcaggg aaaagcccgg
     7621 tattggcgta cagcaatcat agctaaaaca caagaagaat gcgaagctct ttatgctcaa
     7681 ctgccagacg agttgaaaca acaagtccag ctgattttaa atgagagtga ctttatgaag
     7741 cgttccatta ttttacttcc agccttttta gcaaaaggtc tggagtttga ccgagttttc
     7801 ttgtgggatg tagatgcaga acatttttct tctcagcaag acaaattgat cctttacacc
     7861 atgtgtacac gtgcgatgca tgaattgacc ctttttacct ccaataatga aagtcctttg
     7921 ttaaacaata tggatatttc atcttatgag aaaaaagtac tgtaagtaaa aagacaactg
     7981 ctatcattaa tgttagcagt tgtcttttta tttacaataa taattgcaga aaaagtaatt
     8041 caatcattta aggtcattta ttctatatat aaatccaaat ctgtttcagg atattttaat
     8101 ttgacaagcg aaataatcat tttcagttct tccatgtttt ctggatattc tgggtcaaat
     8161 accttgctat gacggccaat cccgccattg tcgatcaatc ggtaaattcg attccattca
     8221 atgaattggt caattgattg gaaattgctg attggatagc cgccacaatc caaaatctct
     8281 gatacacgaa atgctaggta tgcactatcg atcactttat tttttttcgt gtctatcttc
     8341 tcatttcttt cgataagaat tttttcagga cgatgtttca agctccactc taatagtaga
     8401 aagaaagcct ccatccgata ctcttcttca aataaccgaa aggctgaatc agaacgacga
     8461 aaaagcagcg gaatcagata attttgtcta taaatcagca ttggtgcgcg gtccaccata
     8521 cactctaaat aacggttcag ataccctgta ccgacatagt ttcctaatcg atagccttca
     8581 acgccgaatc ttgcgtattt ttgcgctaat tttgaacaat cagttacgtt cacagctaac
     8641 tcttctactt tgcggtaacc gcttaagata cgttcaaaag gttctaaagt gatttttgct
     8701 tccatattct tccccgtcca tcttccacat acttcatatc atttcacatg atacttatgc
     8761 tctcatcata ccacaggttg aattagaaaa gaaaataaat cacacaaaaa agcaagacaa
     8821 tctgttagtg agattgtctt gcttttcgaa aaagactgaa tacttttcgt taaaactggt
     8881 tgcttagtct tctaatgcca cattatggaa cacatgctgt acatcttcta gatcttccag
     8941 tgcatcaatc attttttcaa atgtttcttg atcttctgga gatagtgtta cctcgttttg
     9001 agggatcatt tggatctcag cgattgaaaa atcagtgatt cctttttctt ttaatgcttc
     9061 ttgtatacta tggaaatccg ctacttcacc gtaaacgatg atttgtcctt cttcttccat
     9121 tacatcgcga acatcgatgt ctttttccat taggtattca aggatctcgt cagcgtcttc
     9181 tcctgcaaaa gcaaagattg ctgtattgtc aaacatgtaa gaaaccgctc cggaaacacc
     9241 catgttcccg ccatttttcc caaatgctgc ccgtacgtct gctgctgttc gattcacatt
     9301 gttcgtcaat gtatcaacaa tcaccattga gccgcttggt ccaaatcctt catagcgcaa
     9361 ttcagagtat gtttcatctc ctgcaccttt tgctttttcg attgctcggt caataatatg
     9421 ttttggtaca ttataagttt tagcgcgatc aatgacgaaa cgtagttttt ggtttgcatg
     9481 cggatcagga tcacctgatt ttgctgccgc gtagatttca atcccaaatt ttgcataaat
     9541 ccggctatta ttagcatctt tagcagtttt ctttgctacg atatttgccc atttacgtcc
     9601 catattttca cttcgctttc tgatttgata gtttcataac gcaaaaacgt cctgttccat
     9661 tatacttgtg aatcacgatt ctgtgaagct ttttttcttt cttccttcat tcttctcatt
     9721 tttagtcaat tatctatact cggattggga tttgcccgaa attctgttcc ggaatattgc
     9781 ctcccaattg atagatttga tcacttctaa ctaacagttc cgcttcttgt gcctcttttt
     9841 ttccgattcg ggaataaata gaaatctctt ttttttcttt taagaatctt cttccagcat
     9901 cggtaaatcc aagtagtcga agataatttt tattttgttg gacaggaatc tcttgctttt
     9961 tgatattcag caagacataa caagataatc gttggatccg tgtccatgta tatctttttg
    10021 tttttacctt tgacacaaaa tcctcaaaac tatcagcagt tgcagctgct gttttcatgc
    10081 gcgattctat tccttcggtc atctgataga tatctgtcag ttccagcaaa gtagaagata
    10141 acaaccgata acgcaaatac ggccaataag attcccaagt ctgcagcgaa caagttgaaa
    10201 gatcttcttc tgtctgaggc ggcacaagtc cagctattct ttttccttct tctaacgcat
    10261 ggcgaatggc tgttgcactg gctatcctct gatctgtgat gattttttca tgatagcctg
    10321 cacttttacg tcggatcgga tgtaattcca tagggtggac atacttggca ttttcctttg
    10381 cataacttaa tcccaagata tgatttggcg aagaaaaatc aagcgtaata tcgggataca
    10441 gactgcgaaa aacctcagtc attttttgcg gataactata cgtttgatca gacagttctt
    10501 gaaatgcctc ttctatccta tcttgattct cttggacaaa gcgtccgaaa ctgctgtaat
    10561 caaacgaagt ctcttcttct gttccaaaac acaaactgtc acagtccagt gtttgcaaga
    10621 tttttactgc acctttagca aaatagtcag ctgactgaac cgcccattcg acgggaagct
    10681 ccactactaa atccacacca ttttgtaaag cttcattcgc gcgatgccat ttatcgatga
    10741 tagccggttc gcctctttgc aaaaaattcc cgctcattac agcaatgacg acctctgctc
    10801 ctgttatttt tcgtgccatt tccacatgat agcgatgtcc attgtgaaaa ggattgtact
    10861 ctacgataat cccgcagctt ttcatgcgca tcctccttca tagttatcga taaaaaagaa
    10921 atagagacaa gtaaaaaaag gcaaaaatcg gaaaaaatac ccgaactata taaactattc
    10981 tcgcaaacac aatacttctg cgacgaaaag ctgcactgca agagcaatgc ctatatcgtt
    11041 cggatatata atttatgttg aagatttatt cttttcttgt taactcgagc aggcaaaacc
    11101 gctagtcttt gtatgctaca aagaaccagc gctttgattt ctctgatgct gcttcgtctg
    11161 taaaatcaga aaatgcttcg actttcgaaa agccgctgtt ttccagcatt cttaaataac
    11221 tttccaatgg ataggtacgc tcttgatgta gttcatcttc acgaataaat cgaccagacc
    11281 cttcttgttc ttcaacgaaa aaggtaagaa aatgttcgat gctgtgtttc tcgcttcctg
    11341 gataactatc ccacaaaaaa gcaaaattat ccccttgata atgatagcta tattctggaa
    11401 aaacctcatc tacttgatag gtggagtgta cgtcaaataa aaatcggcca ttttcttcta
    11461 acgcttgata gacttcatca aacacttgct gtacctcttg tctatttttc atatagcaca
    11521 gcgaatcaga aaaacaggta attgcctgat attgtccgat ttctgataaa tcgagcatat
    11581 tgccttccac aaattggatc gatgcgtctt cttcatatgc tcgctggctg gcaatcatca
    11641 acatctcttc tgaaaggtct aaccctgtca ctttatatcc atcttttgca aaggcaaccg
    11701 ctaatgctcc agtaccgcat gccagttcca aaatattttt tgtgtcttct tgtaaatgac
    11761 gtttcgaaaa agctaaccat tgctgataaa gtgtttcgtc catcacttca tcgtaaatga
    11821 aagcaaatgt ttcataagcc atatgttaat ccaaccattc actaagatcg actagcggag
    11881 catctgacca tagtttttcc agattataga aatttcgttc tggcgcatgg aaaacatgta
    11941 cgatcacatc tccaagatcg atcaataccc attttccgcc ttcttttcct tcaatacgtt
    12001 ttacttcata tttgttttct tcttctttat caatgatttc ttctgtaatt gcgttgattt
    12061 gacgttcgct atttgctgaa cagatcatga aataatctgc caacaaggaa acatttcgaa
    12121 cgtctaaagc gataatgtct tctgcccgtt ttgaatctgc tgcttcaatc gcaattttta
    12181 attgttcttt actatctatg atgatttcct cctaatatga gcagcttatt tttttgctac
    12241 ccagtaatta tatgtttcga ttgtttttgg ataaatctgt tgttcttgcg caagcaaatg
    12301 ggccaatgta tgttttgttt caaatgctac agcttcgtct agatcagccc atgcaatggc
    12361 tcgtgcatct tgtaccccag gaaaatctcg acctggttca atatagtctg ctacataaat
    12421 gattttgtca aggagactca tcttagcagc cccagtcgta tgcacccgga ttgcatgaag
    12481 aatctctgca tcggtgattc ctaattcccg ctctacaaac gaagcgccaa caagaccatg
    12541 ccagattgca ttattataat tcaataactc cggatcaaaa ccgtctctta cgattaccat
    12601 tttgaactcc tcatctggtc gttcttttgc ataatcatgt gtcaatgcag caatactagc
    12661 tttttcagga gaagcaccgt attttttggc aagagccacg gctgtttctt ctactccaag
    12721 cacatgcttg aagcgttgtt cgctcattct catctgtatt ttttgcatca ggttttctct
    12781 agaataagca gtatattcat tactatagtc catattcata caacccttca ttttgaatat
    12841 aatcaattac tttatctgga acaaggtagc ggatagaaca gccttctttg atcttttgac
    12901 gtattttcgt ggaactgatg tcgatctcag gtacatctac ccaaattaca ggatatggag
    12961 tatccgttgt gtatcctggt cgacgtatac ccacaaagtt gaccatagaa gtcagctcat
    13021 cgattttata ccattttggc aaatattcta ccatatcccc accgataata aagtaataat
    13081 ctgtatccgg attgttttgc gtcaattcct tcatagtatc ataggtgtaa cttttcccac
    13141 cacgagctaa ttcaatcgtt tcaatctgca aaaaaggatt gtcttccacc gctaattcca
    13201 acatgttgag tcgatgtttt gcatcaatcg tctgtttctc atcaacatga ggtggctgat
    13261 aagaaggcat caaaaatacg cggtcaagac ctaaattccg gccagcttgt tcagccatca
    13321 ctaaatgtgc aaggtgaacg ggattgaatg ttccgcccaa gatccctact tgctttttct
    13381 tctctaagaa ttgaggcatt tcttcaggaa ataattgtga acgaacaaat gtcttagctt
    13441 gcgtattcat catggtcact ccttatattg cttttacttc ttttgatagc ctttgatact
    13501 tttcttttgt ggaaggtttg tataatacaa taacacgtcc aatgatttgg acgatttcgc
    13561 aatctatttc tttttctaat acttgagctg cttcttctgc aatctcatct gtattttgaa
    13621 gtaacgatac tttgatcagt tctctttttt ctaaagcttc ttcaatttga acgatcattg
    13681 ctgaattgat cccacctttt ccgatttgaa aaatcggctg aagatgatgc gctttgctac
    13741 gtaaaaaacg cttttgtttt cctcttaaac tcataatttt ccttcttcct taaatcaatg
    13801 ctttgcgttt caatacatct acaccctttg gcgcccagcc cgcaattact actggcgctg
    13861 ttacagtgat ccagcctaga ccggcaaaaa cgatgtctgt tttttctttc acagaaaact
    13921 caaaacgaac aagctccgga aatgcctcga cttcatcagg acgaggaggc tgtaataatc
    13981 cacctacatg tttttgataa aattcgtctg ctttttccag tttcgttcga tgaatattta
    14041 catcatttga cacataagca ataaatgaag cacgattgcc gctgataaaa tcaaatcttg
    14101 caagtccgcc caagaaaagt gtttgccctt cattcagctg ataaacttta ggtttgattt
    14161 cttttaacgg tgcaatgatc cgcaaatctt ttttacctaa gtagtgcgcc atttgatggc
    14221 gatggataat tcccggtgta tcaatcaaaa aatgcccgtc atctaaagga atctcgattt
    14281 tatccaaggt cgttcctgga aactgcgaag ttgtgattac atcttgtact cccgctgtat
    14341 ttttgatgat ttgattgata agcgtcgatt ttccgacatt tgttactccg acgatataga
    14401 catcccgtcc atcgcggtat tcttcgattt tttctaatag ttccgtcatc tcatgagatt
    14461 tcttagcact agttaataat acatctactg gacgaattcc tgcttcgtaa gcacgttcct
    14521 tcatccattg tgttaattta gtttttttca atgatttagg taatatatcc actttgttcc
    14581 caactaaaag tactggattg tcaccgacaa aacggtgtag tcctgggatt agtgaaccat
    14641 tgaagtcaaa aatatccacc acattgacga tcaatgcatc tttttgccct aattcattca
    14701 gtaagcgcaa aaagtcgtca tccgtcaatg agacatcttg ggtctcatta taatggcgta
    14761 atctgaaaca acgctgacaa tagacttccc cggaagctaa acctttttcc aaagccgttt
    14821 gcggcgtgta tcctaattca ccgggtttgt ttgtttgtat aacggcacca cagccgatac
    14881 attgtaattc ttctgtcatt cgattccacc tcgccagccc atatcagggt gttttgctaa
    14941 tagataggcc ataattcttt tttcccaaaa acgattaaat tgtgtcttcc aactatctgt
    15001 tgcgataatg ggttttacaa ggatgctacg tatccctgca cgattggctc cacgtatatc
    15061 agtcataatc tgatcgccta ccattactat ctcatcatct gctaaatcca attgtttctt
    15121 tgctatgttg atccctcttg ctaaaggttt cattgctcgt gcaacataca aaagatcaaa
    15181 tttttcaatc gctcgcgcga cacgttttga attattgttt gaaacaacaa caacagggat
    15241 tcctgcattt ttcatttcta aaatccaagt aagcaactct tctgttccat ctggattatt
    15301 ccatgcaatc aacgtattgt ctaaatcggt caatactgct ttgatgccta gattctttaa
    15361 ttgggcaggt gtaatctgat agatggcttc taccatccat gttggtttgt aatttgaaaa
    15421 catgctgtct cctcatctgc aaaaaatgaa ggagcacata aagtactcct caaccccgta
    15481 ttatactgca tatcagcaaa aaaaactacc tctatactcg ttcgtctttc agaaaaagca
    15541 tttctttaca aaatgaccaa aaataaagat tgatcacaga aaaatttaga aacaaaaaac
    15601 aactaatatt ggcggaacct cttgtatcgt ttttccagta atttatgctg atctaatcga
    15661 acaagttccg tcaatctttc aatcaacgct tttttcataa tgtgtgcgac tttatatcgt
    15721 ggcaactgct cgccgttgaa cgtttcagga ataactcgat caacgattcc taactcgcgt
    15781 agttctggcg cagtaatttt catcaactca gctgcttctg cggcgcgact accgtctttc
    15841 cacaaaatcg acgcaaatcc ttctggagaa agcacagcat aaatcgtttc ttccagcatc
    15901 cagacttcat ccgctaaacc tagagcaagg gcaccgccac ttccaccttc accaatgatg
    15961 atggcgatga tcggtacctt taaatcagac atttcgagca gatttctggc aatagcctct
    16021 ccttcgccgc gctcttctgc tcctacaccg caatatgcac ctgctgtatt gatcaaggta
    16081 atcaccggtc tattgaactt ttccgcttgt ttcatcagtc gtaaagcttt tcgatagcct
    16141 tcaggatggg gagaaccaaa atttctgcgg atattttcct gcagatcccg tcctttttga
    16201 ataccgatca ctgttaccgg tttgccatct aatcgagcga ttccaccaac aatcgcttcg
    16261 tcatctgcaa ataaacgatc accatggcat tcctcgaaag aatcgaagat ctcttcaaaa
    16321 aaatccaagc ttgtccatcg ttctttagct cttgctaact gaacgacatc atgtgctgtc
    16381 ttatccacga tctatccatc ctttctgtgt atgcaattca agaagcgtat ggattttctg
    16441 tttcaattcc atgcggggaa caatctgatc gacaaaacca tgagataata agaattcagc
    16501 cttttggaaa tcttctggaa gttcctgctt gatcgtctgt tcgatgacgc gtcttccagc
    16561 aaaaccgatc aagctttggg gttctgctag aataatatcg ccttccattg caaaactggc
    16621 agttactcct ccggtcgtcg ggtccgttaa aacagttaga taaaaaagac cttcattgct
    16681 atgccgtttg acagcagcag agatttttgc catctgcatt agagaaaaga ttccttcttg
    16741 catccgtgcg ccacctgaag ctgtgaacaa gacgactgga agactttcct ttgttgctcg
    16801 ttcgaataat ctggtgatct tttctccgac aatcgttccc atacttccca tgataaaatt
    16861 aggatccatc actccaataa tgaagggaat cccctgaatg gttgctttac cagtgaggac
    16921 tgcttcatga agtcctgttt tatcttgcat ttttttaatt ttttccgtat attcgggaaa
    16981 tgctagcgga tctttggtta caagatccgt atcccattct tcaaaacttt tttcatcaat
    17041 aatcaaagct agacgctgcc atgcaccgat tcgaaaagcg tatccgcaat gagggcatac
    17101 tttttcttca ccgatgtctt tcgtataaag gatatgtttg caatttggac actttgccca
    17161 catgttatca ggaacagatg gcgagttagc aggtttgctc tctctattag gattgatccg
    17221 aatatatttc tttttcttaa acagagccat ctatgcgcct cctatctccg tttctggtgt
    17281 ccaattcggt aaaaattctt cttgtaaaaa tgctgtgctg taatcacctg ctatgactgc
    17341 aggatgacta atcaaatcca tctgaaattc tgcgttcgtg atgattcctt ctgtgataaa
    17401 ttcacttaag gctcgctgca tcttcattaa tgcttcaaaa cgcgtatttc catggacgat
    17461 tatttttgca atcattgaat cataatatgg cgggatcgta tatcccgggt aaactgcact
    17521 gtctactcgc agtcccatcc cacctgctgg aagcagtaag ttttggatct tccctggaga
    17581 aggagcaaaa tggaaagcag gattttctgc attaatccgg cattcgattg cgtgtccttg
    17641 gaatactact tcttcttgac acacattcaa aggctcaccg gctgctatct ccacctgttt
    17701 ttttactaaa tcgattcctg tgaccatttc tgttactgga tgttccactt ggattcgagt
    17761 attcatttcc atgaaataaa aatcgccttc ttcatccatc agaaactcga tcgttccggc
    17821 attttcataa ttgactgctt tagctgctcg aacagcagcg tctcctagtg cttctctttt
    17881 tgtttgtgag atcactacag aaggagattc ttccagtact ttttgattgt tgcgttgtaa
    17941 agaacaatct cgctcgccta aatgaatgac atgaccaaat cgatcaccta agatctgaac
    18001 ttctatatgg cgagcaggat aaatgatttt ttccaaatac atatcatcat ttccaaatgc
    18061 cgctgctgct tcttgctgag ctgaagaaaa atgctgtggg agctcttctt ttgattgaac
    18121 tttccgaatg cctttcccgc caccacctgc agctgctttc agcatcactg gataaccaat
    18181 tctgtctgct atttccaatg cttcttctac tgtagataat acaccagtac ttcctggaat
    18241 gactgggaca tttgcttctt gcatcaattg acgcgcattg atcttatttc ccattgcatc
    18301 gatcgttgca cttttaggac caataaatgt gatattgcac tcttcacaca tggaggcaaa
    18361 tcggctattt tcagataaaa aaccaaatcc tggatgaatt gcttcggctt ttgtcacgat
    18421 tgcagcgctc agtacttcct gaacattcag atatgaatca acggcttttg ccgggccaat
    18481 gcagattgct tcatctgcta actgtgtatg aagtgcttcg gcatctgctt ctgaatatat
    18541 cgctacggtt tggatcccta gttcgcggca tgcacgaatg atacgtacag cgatttcacc
    18601 gcggttagct atcaatactt tagaaaacat atttcaccta tccaatcata aaggttaatt
    18661 ccgcttcggc tactttttta ccgtctactt tagcgattcc tttgcctatt ccggctgaag
    18721 ctttcacttt caatatctca acttcaaggt ataaggtatc tcccggtgta actttcttac
    18781 ggaattttgc tttgtccaat ccgccgaaat aagccgtctt tcccttgaat tgttcaagag
    18841 aaagtaatgc aactgctcct gcttgagcca ttgcttcaac aatcaatacc cctggcataa
    18901 caggttcttc tggaaaatgt ccttgaaaaa agggctcatt gatcgttaca tttttcttag
    18961 caacgatccg ttctccttca accatttctt ccactcgatc aatcaaaagc attggataac
    19021 gatgtggaat gatttctttt atttcttgta tgttcattgt tttccctctt tcctgctttg
    19081 cttatttttg ctgaatccta aagagtggct gtccatattc gacaactgac tcgttttcta
    19141 caaggatttc tgtgatcact ccgtcggcct cactagttat ctcattcatc agtttcatcg
    19201 cttcaataat gcagataact tctcctttgg caacttgatc acctactttt ttgaaagctg
    19261 gtttgtcggg cgctggttgc agataaacga ttcccacgat tggcgaaaca acctcgattc
    19321 catcaaatac tgccgattct ttttctttca ttatttctgg agcagttgtt gcttgttcag
    19381 gtatgacatt gactggctcc gaaactggca acgtttcgtt tgtttgaacc atattcatgc
    19441 ctcgacttgt tttattttta ttcatataca attcaaattg accttcacgc aaatcgaatt
    19501 ccgttagcga ggattgatca aattgactga ctaattctct gatttcattg atatccaaaa
    19561 ttattcctcc cagcgtttca tgcacagaac cgcattatgt ccgccaaacc ctaatgaatt
    19621 actcattgca taagtaaaac ttgcaggttt gctttcgttt ttgacaactg tcaagtcaat
    19681 ctcaggatct tgtttttcta cattgatcgt aggtgggata aattgatgtt ctaatgccag
    19741 caaagttgcc aaggcttcga tcccgccggc tgctcccaat aaatgtccag tcatcgattt
    19801 cgtgctgctg acgtaagtat ctctgaaatt ttcacctaat gcgatttgga ttgcttttgc
    19861 ttctgcctgg tcattggctg gtgtacttgt tccgtgagca ttgatatatc cgacttgttc
    19921 cggctgtaca ccagcttctt ccatcgcaag cttcattgct ttagatgcac cagaaccatc
    19981 tggagtagga gctgtcatat ggtaagcgtc acagtttgca ccgtatccaa cgatttctcc
    20041 atagattttg gcaccgcgag cttgtgcatg ttctaaagat tcaagaacta gaacgcctgc
    20101 tccttcaccc atcacaaaac cattccgatt ttcgtcaaac ggaatcgaac cacgattagg
    20161 atcagttgat tctgagagtg ctgttaaagc tgcaaatcca gcaatcccca tctcacaaat
    20221 cgttgcttct gttcctccag caagaatgac atcagaatag ccatgtttga tgttacggaa
    20281 tgcttcccca attgaattcg ttccgctagc acaagctgtt acgatagctg tacaaatacc
    20341 ttttgcacct acacgcaaag caatatttcc agctgccatg ttggcaatcg ccattggtac
    20401 gaacattgga gaaactcgtt ttgcaccttt ttcatgtaga cgaataactt ggttttcgat
    20461 tgttggaaga ccaccgatcc cagatccgat gatgacgcca aaccggtctt gattcatttt
    20521 ttctttatct aaaccgctca tctccattgc ttctaaagca gcgtagattc catagatcga
    20581 aaacatatcc attcgtttgc tatctttttt aataaaatat ttatcgtaag ggaaatcttt
    20641 gacttcaccc gctaaggtaa tccctgtttc tgaagcatca aatttagtga tcggtgcgat
    20701 gccattcgtc ccctctttta agctattcaa aaagctgtct gcgtcatttc caataggtga
    20761 agtgactcca tagccagtaa ttaccactcg attcatttgt ttgctctcct tttcttatcc
    20821 gtgcatgacc aatccgccat cgacattgat gacttgtccg gtgatatatg gattttgtgc
    20881 taagaaaact gctgtttgtg caatatcttc tacttgtccg aaacgctgca tcggaatttg
    20941 cttttccgct tgttccttga ctttgtctgc taaaacttcc gtcatatcag ttgtgatgaa
    21001 tcctggcgca atggcattac atgtaatgcc tctagttgca gcttctcgtg ccacagattt
    21061 tgttaatcct actacgccag ctttactagc tgcataattt gcttgaccaa tatttccaat
    21121 caacccagat acactagaga gattaatgat ggccccttct cgctgcttca tcattttttt
    21181 caatacgtgc tgcgtcatat tgaaggttcc aatcaaattg atatccagac attttttgaa
    21241 atcttctgca tccattcgca tcacaagttt gtcatttgtg attccggcat tgtttactaa
    21301 cacatggatc gatccaagct tttcttctgc ttctttgatc atttgtcccg ctttctcata
    21361 atcagagatg tcaccggaaa ctcctacgca ttttacaccg aatgcttcga tttcttctat
    21421 cttttctttt ggaatctctc cgcgaccatt caaaatgata tttgctccag cttttgcaaa
    21481 agctaaggcc atcgctttgc cgattcctct tgtactacct gtgataaata cattttttcc
    21541 ttttacatcc ataacgttcc tccttatagt attgctattg tttcatcaaa tgtctgctta
    21601 ttctcaacac gcagaactgg tattgtttta tcgatttttt tcataaatcc gcttaatact
    21661 tttccggggc cgacttcgat gacttgttcg atgccgattg tttttaactt atggatactc
    21721 tcatagaaac gaacaggttg catgacttgc tgttcaagta gtcccgcgat tgtttctttc
    21781 ttcattattt ccgttgttgt gttgcttatg ataggaaaag aaggttcaga gaattgaatc
    21841 tgacttaggt ccttcgccag tttttttgct gccggttcta aaatagctgt atgaaaaggc
    21901 ccgctgacat ttagagggat cattcttttg aacccttttt cttttaatag taaaacagct
    21961 tcgtctactg ctttttcttc gccaccaatc acgatctgct gaggtgtatt ataatttgca
    22021 ggagatacga tgccgtactt gctagcttca tgacagcttt cctcgatcgt ttcaatcggc
    22081 gcattcatca ctgcaaccat tttcccgctt ccagctgggg cagcttctgt catataagcg
    22141 cctctttttg ctactaacgc aactgcttca gtgaaggata gcgcattact agctactaat
    22201 gccgaatatt ctcctaaact cagtcctaaa gctgcatctg ggatgattcc atgctccttc
    22261 aataagcgat aatacgcgat gctgactgtt agaatagctg gctgtgtata ttgcgtctga
    22321 tccaaacgtt cattttctgt aaagcaaagt tcagccatat cgtagccgag aatctcactt
    22381 gcttcatcaa atgtctgctt tacaacggct tcttcatata aatcttttcc cataccttga
    22441 tattgtgcac cttggccact aaataagaat gctgttttca tgtttttctc caatatatcc
    22501 agaatatatg tttgattctt cctattcaaa aaggccgaga cattcctatt tcaggaaatg
    22561 tcccagcccc caacctaatt ttaatgatta tgcagtttgt ttttctacgt aagtcactaa
    22621 atcttgaacc gttttgattc cgtcttctgt ttcgattttc acgtcaaatt catcttcaat
    22681 ttcattgatg atttggaata aatccaagct gtcagcttct aagtcttctt ggatgtcagt
    22741 agtcaatttg atttcttcag tttcttttcc taattcttct gcgatgattt cttgaatttt
    22801 ttcgaatacc ataataatat attcctccga tattgtttgc tttttatttt tataatttaa
    22861 cagtctacaa ctccatgaga atacttcccc atgttaaacc tccgccatag cctgtaaata
    22921 ttactttctg ctgttttcct aaggtgatct ttccactaga aactgcttca tccaataaga
    22981 tagggatgct tgcagcagat gtattcccat atttatccat attggtcagg aacttttctt
    23041 gcggcatctt tacttttcgg gcaattttct caataattct gaggtttgcc tgatgcaaaa
    23101 gcagataatc aactggtttg tctttcatga cttcttggat attgtttgca acgtctcgta
    23161 cggcaaaatc aaaaatatct ctgccagtca tttgcaaata atacgaacct tctgaatctt
    23221 ctgaatagaa cggattttta ttttgctggt agccagcggt caatgagcta cttctggtac
    23281 cgtcagctgc taatttttct ttcagcaatt ttttctgtgg attcgcttct aagagaatcc
    23341 cacctgctcc atcgccaaac aacacagcag tcgtacgatc agaccaatcg attagcttag
    23401 atagtgtttc accaccaatc accagtccct tagttgagcc gcagcggatc attttttcag
    23461 ccatgcttaa cgcataaaca aatcctgagc acgcagcact gatatcaaaa gcgatagcat
    23521 gagttgctcc caatttccct tgtacttgtg ctgctacaga tggtgtcgtg taatcgggcg
    23581 acattgttgc tactaaaata aaatccaaat ctttagggtt tagatttttc tgttttatca
    23641 atttttccgc cactaaaatg cataaatcag aagtctcctg atttgttaca catcttcgtt
    23701 cacgaatacc cgtccttgac gaaatccatt catcagaggt atccatcatc ttggaaagat
    23761 cattattagt aataatcttt tcaggaagga cgctggctgt tgcagtaatc attccaaatt
    23821 gttccattaa atatcctcac ttatagtctt gcaagaagtc gtgcaagttt ttcagtgcat
    23881 ccagcaaggc atgctgttcc tccgtagcca ttccgtctaa gatgttcttg accatttcac
    23941 gatgaaaatg ctgatggacc cgaaatagta actttccttt tttcgttagt cccaacttta
    24001 caacgcggcg gtcatcttca ctgcggatac gttcgacata gccttttttt actaacttgt
    24061 taatagcagt ggtcaaggtc ccaacagtaa ttgatagttc tttggctact tctgaagttg
    24121 tttttttctt gtacatcccg attgcctcga tcgtgtgcat ctctgtgatt gacagatctt
    24181 tgaattgaga ttttttcagc tcagactctt cgattgtcaa aatatcattg aagacactaa
    24241 caagataatc atttactgtt tttaaatctg gttccatgtt ccaccgtcca ctaattagtt
    24301 tgattatcaa atcttttgat gttcaaacta tatcttaact tttttcaaaa tgcaacctta
    24361 aaagatcatt tttttactat atgagctttt tttaagattt tactcattta aaacaactga
    24421 taaatcagca tttataattt cttttattag aaaaatcatc tatctcacat attttgatta
    24481 tcaaagtatt ttctgtgaac agaactatag aattccctaa aacttgtcca ttatttcaaa
    24541 atttgttata gtctttttat tcaaagcgta aaaggagatc ctatgttaaa agaaaaaatg
    24601 aaaactctcc ccatgctgcc gggtgtctat cttatgaaag accgagatgg taaggtcatc
    24661 tatgttggta aagcaaaacg gctcaaaaat agagtaagca gctattttca tcagaataaa
    24721 cagcattcaa aaaaagtatt acgtatgatt catcatataa cagattttga tttcgtcgta
    24781 gtagacacgg aattagatgc tttattgctc gagtgtcaat tgatccaaca ttaccgtcct
    24841 ttttataacc gacaaatgaa ttatttcagt aattataatt atgtacatat taccaataag
    24901 ggatttgtat taacagatac tcctacggct cgaacatacg ggccttttcg tctctataaa
    24961 aaaatgccgt ctattttgcg tataatggaa gaaacttatc aaatgccttg gttgtctgaa
    25021 attagtttgt tagctttgcg agtccagctt cctgacctac aggaaatgac ttttgaacag
    25081 aaaaaaaagg aactccaagg gctgtttcaa ggccgaaata aaaagctact gacttacttg
    25141 aaaaaaagac aacaacattt catctatcag ttgaatttcg aaaaagccgg tatgcttcaa
    25201 aaagatatcg aattagttac ttattttatt agacggatcc aggaacaaaa gcagttttta
    25261 agaacaccat cgctcacctt cagcatgcca ttagcagcag atgagagtca gaaaaagcac
    25321 tacctcgttt gttatggtca acttgcagaa acaatgatta cctctggtga cgtttctcct
    25381 gacttctatt atgaaaagaa agaagctcat ttatctttga aacgtcaatt gtcaaaagaa
    25441 gaaatcgatc ctgtacaaat cttgatcagt taccgcaaaa aattagaaaa agaacagttt
    25501 gaaattgaac tgttaaacaa aaaagaagct gaaaagcaac taaattagtt gcttctcggc
    25561 ttcttttagg catcttcaac ttcaaatgtc aattatccta aaatttattg gattaagtca
    25621 tagatttcca tcgctacgat atcgatatta tcaaattcgt attgctgatg agtatctgtt
    25681 tcagttaatt caaatgtatc ggtcgcttta tcataagtta ctgtacactt ctctacgcct
    25741 tctttttcaa aacggcgaac ttgcagttca ttatctgctg actcgaccat agcctccagt
    25801 cgttttatga ttgcgactaa ttgtgaatgt gtcatcggag gtcctccttt tcttatttct
    25861 gttatattgt atcaaaagca tagcctactt gcattgattt tctctaaaaa aactcgtttt
    25921 ttatgatttt ttttctggta tttttccttc aggccaccaa agtttgatca aagcttgggt
    25981 tccgtcattc tcaaaacctt tatctgccaa ctcttcatat agtttcaatg cttgggtagt
    26041 tgctggcaat acaagatcca ttttttctgc ttcttctaat gcaatcttta aatctttgat
    26101 aaaatgtttg acaaaaaaac caggtgtgta atcttctttt aaaatacgag gagaataatt
    26161 gcttaaagac cagttagctg cacttccacc tgaaagcgtc tcaatcactt tctttaaatc
    26221 aagtccgttt ttttgtgcat agacaagcat ttccgtcatt cctgtcatcg tacctgcaat
    26281 catgatctga ttagccattt ttgtatgctg acctttccca gcagaaccat gcaatgtaaa
    26341 tgtcgtacca aattctttga ataaaggcag tacttgatca taaacggctt cttctccacc
    26401 gaccatgatc gtcagtgtac cattctttgc tcctaagtca ccaccggata ccggtgcatc
    26461 taacgcatct gcgccttttt cttttgctgt ttttgcaatt ttttctgcca ggcttggtgt
    26521 acttgtagtc aaatcaacta aaatatgtcc tgagatatct gcagaaaaaa tccccgactg
    26581 tccatagtag atttcttcta catctttggg gtaacctacc attgtaaaga gaatattact
    26641 ttgttctgct accgcttttg gtgtatctgc ccagatagct ccttctgtta ctaaaggatc
    26701 agcttttgct tttgtccgat tatagacagc tacttcgtgt cctgcttcta gtagatggcg
    26761 tgcgacagca cttcccatga cgcctgttcc gataaaacca agtttcattt tttcgacctc
    26821 ctaaaaatca aaaatccttg cttctatgct agtatacgct agaaacaagg attttttcat
    26881 aaaaaatgtt tgaattttat cttttaaatg aatttaattt tattttgatt cgttcaatcg
    26941 agtcttcttc ttcttttctt cgcatctctt cttcatgata aacaaccatg tcttgataaa
    27001 attcgatcat agcttcgccg aaatgtgtag atgaaatatc ataaagttta gcttctaaaa
    27061 cttttggatc tttaggaact tcactggaaa tataatcaat aagtgctggt gcaaagtctt
    27121 cgtcggtttc aaaagttttc cctaatgaag gatgatcgaa caatcggttc aaataagcat
    27181 ttccttccac gacacaaggt gttccggctg ccatcgcttc tgtataagta agcccttgtg
    27241 tttctgaagt agaagcactg acaaaataat ctgccgcttt ataataaagt gctacatcat
    27301 tgttgtccac ttctcctgtg aactgcacaa attcttctaa ctgcagttct gagatcaatt
    27361 cctctaactc ttctacataa ggtccttttc ctacgataac taatctggcg ttaggataag
    27421 cggagatgac ctgaggaaac ccattaatca aggcttggat atttttctca taagaaatcc
    27481 tgcttaaaga aagaatcatc aaatggtttt cctgaagacc tagtgactcc cttaatttgc
    27541 tgatatcctg ctgagtgata tcagaacgtt tgaatttttc aatgtcgata ccagtaggaa
    27601 tcacgcgaag cggtgctatt acaccatagc tgcgtaatgt atcaataaca cgttcacttg
    27661 gacaaactac acctgtcgta tgatttgcaa ataatctaga caagtatttt acatgggatt
    27721 gcctgatcac ttttccttta ccgatataat gaaggtagtc ttcatacatc gtatggtaag
    27781 tatggatcag aggtattttc aacttcttag ctaccatctt cccaagtatc cctgctccga
    27841 attctgtatg ggtatgaata agatctagtt ctagttcttt ggcaaccaaa taagcatacc
    27901 acatgcctct aacaacaact cttcgatcct tgaaagaaat aaatggtaca cttggcattc
    27961 gaatcacatc tttttccaac ttatccgcat taggatcggt cgttgtaaaa atatagactt
    28021 catgcccatg tctttctaat tcttgtttta atgttttgat cgatgtagca actccgctga
    28081 cttgcggaaa gtaggtatcg gtaaaaaagc cgattttcat gtttttcgcc tccaaactag
    28141 tccttgacct gatttacttt ttttaatttg ctagagactt tttcattcag tacactttcg
    28201 tatacatttt taagttcttc accgatttta ttgatacttc gctcttttgc tgtttgataa
    28261 ccggcttgtg tcgtactcgg tagttcttga ttggtaatct gttggatcag tcggataaat
    28321 tcttcgtttg tcttccccat atagcagttc ttgctatctt ctaaccagcc attatacact
    28381 ggaatatctc gaacaagtac gttttgtttg cttgctaaag cttctaaaac aacgatccct
    28441 tctgtctctt cataagaagg gaaaaagaat agatctgcac ctgaatacgc accttctatg
    28501 atctctccac gaatgtaacc aggaaacagg acattttcag gatgatcttc ttttacaatt
    28561 ttccggatgt tccgaggaat cgagtacatg ggaacatgac caaaccaaat aaatgtgtat
    28621 tcgggcatct tcttagcaat ttctacgaaa tcaatgattc ctttacgttc gaaaaacaaa
    28681 ccgacacaaa tgattacttt ttgatccgga gacagtttga aatattctct gaacttttgt
    28741 tctttcattg gatcagggtg atatttctct aagtcgatcc cattggaaat agcttgaatc
    28801 ggcacacgga taccatagct ttccaacaac tgttttgaat aaggtgttgg cgtgatcaga
    28861 tagtccgctt tttggtacaa tcctaccaga tattttttta cgataggaga aagctggttc
    28921 gatccgataa aggaatttcg gaaatcttcc tctgtagaat gcgcatgata gacaactttt
    28981 tttccttctc ttctggcttt attgaccata ttatgactat tgattccgta tgtattgatg
    29041 tgcaggatgt cataatcttc tttttcatct agtgtataag agataccgac ttccgtcaga
    29101 gcacgttttt gatgatcaag cgcacgcccg attccagatt ttgcaagcat cttttcactt
    29161 tcaaaatata ataatacctt caccttacgt cccccttcac ttatatagta taacatagcc
    29221 tacaaactgt cactcaaact taagccctat tttt
//
LOCUS       DARBFO010000026        28669 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000026 DARBFO010000000
VERSION     DARBFO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.027"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            39..656
                     /locus_tag="SCK51_001285"
     CDS             39..656
                     /locus_tag="SCK51_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251923.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1676..2845
                     /locus_tag="SCK51_001286"
     CDS             1676..2845
                     /locus_tag="SCK51_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1251924.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3318..3872
                     /locus_tag="SCK51_001287"
     CDS             3318..3872
                     /locus_tag="SCK51_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1251925.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(4045..4518)
                     /locus_tag="SCK51_001288"
     CDS             complement(4045..4518)
                     /locus_tag="SCK51_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1251926.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            4530..5066
                     /locus_tag="SCK51_001289"
     CDS             4530..5066
                     /locus_tag="SCK51_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1251927.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            5106..5477
                     /locus_tag="SCK51_001290"
     CDS             5106..5477
                     /locus_tag="SCK51_001290"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1251928.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            5761..7032
                     /locus_tag="SCK51_001291"
     CDS             5761..7032
                     /locus_tag="SCK51_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG1251929.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            7277..8302
                     /locus_tag="SCK51_001292"
     CDS             7277..8302
                     /locus_tag="SCK51_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1251930.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            8596..9705
                     /locus_tag="SCK51_001293"
     CDS             8596..9705
                     /locus_tag="SCK51_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEG1251931.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            9788..10684
                     /locus_tag="SCK51_001294"
                     /pseudo
     CDS             9788..10684
                     /locus_tag="SCK51_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            10700..11524
                     /locus_tag="SCK51_001295"
     CDS             10700..11524
                     /locus_tag="SCK51_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1251932.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            11556..12866
                     /locus_tag="SCK51_001296"
     CDS             11556..12866
                     /locus_tag="SCK51_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEG1251933.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            13014..14273
                     /locus_tag="SCK51_001297"
     CDS             13014..14273
                     /locus_tag="SCK51_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1251934.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            14283..15155
                     /locus_tag="SCK51_001298"
     CDS             14283..15155
                     /locus_tag="SCK51_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1251935.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            15167..15997
                     /locus_tag="SCK51_001299"
     CDS             15167..15997
                     /locus_tag="SCK51_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1251936.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            16037..18220
                     /locus_tag="SCK51_001300"
     CDS             16037..18220
                     /locus_tag="SCK51_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEG1251937.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            18281..19501
                     /locus_tag="SCK51_001301"
     CDS             18281..19501
                     /locus_tag="SCK51_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HEG1251938.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            19498..20958
                     /gene="gtfA"
                     /locus_tag="SCK51_001302"
     CDS             19498..20958
                     /gene="gtfA"
                     /locus_tag="SCK51_001302"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEG1251939.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            21035..22141
                     /locus_tag="SCK51_001303"
                     /pseudo
     CDS             21035..22141
                     /locus_tag="SCK51_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            22463..22672
                     /locus_tag="SCK51_001304"
     CDS             22463..22672
                     /locus_tag="SCK51_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG1251940.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            22665..22814
                     /locus_tag="SCK51_001305"
     CDS             22665..22814
                     /locus_tag="SCK51_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEG1251941.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            22968..23633
                     /locus_tag="SCK51_001306"
     CDS             22968..23633
                     /locus_tag="SCK51_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1251942.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(23917..25002)
                     /gene="xerS"
                     /locus_tag="SCK51_001307"
     CDS             complement(23917..25002)
                     /gene="xerS"
                     /locus_tag="SCK51_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEG1251943.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            25336..25569
                     /locus_tag="SCK51_001308"
     CDS             25336..25569
                     /locus_tag="SCK51_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HEG1251944.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            25566..25973
                     /locus_tag="SCK51_001309"
     CDS             25566..25973
                     /locus_tag="SCK51_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEG1251945.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(27110..27628)
                     /locus_tag="SCK51_001310"
     CDS             complement(27110..27628)
                     /locus_tag="SCK51_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEG1251946.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            27995..28243
                     /locus_tag="SCK51_001311"
     CDS             27995..28243
                     /locus_tag="SCK51_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251947.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         9852 a         4322 c         5252 g         9243 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgagaacctt gggttttcaa gtgctttttg
       61 gttcattaat cttaaccata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
      121 ggagccttgt aggaatagtt cttattattt ttactgtgac tgcagcaaca gaagcaaatt
      181 attcgcataa aattcagtcg tataaaggtc gtttagttat gaccaaaaaa gatgttacac
      241 gttatacatc ctcaagaact ggaggcctat atgactatgc accattagat gcattgaaat
      301 ataaagatca tttaaagaaa catgaagtaa aagttggtga tcggtgggag aatgcatctc
      361 ataaagcata taattccaaa atagtctata cagtagaaga tgccgatgag caaaatatca
      421 cattacctgt attcaattat tggggaacag ttgtgaaagt aaatgggaaa aagagcacta
      481 tagaaaatga caatggttta gtgtctatta aaggtacggg gaaaagaact atagttgagg
      541 tttcatctac gtatccaact acgatgattc tatctttatt aattagttca ataactttta
      601 tattcttatt tacacgttat ttaataagat gctgcggtga aatgaaaata gattagatag
      661 atattataaa tatgttgatt ctattttcat agcaatggag aaaatacatt agcaatatat
      721 aaaagaaggt atcaagagtc acagtgaatt tattccgaaa agtgaaaaga gcaaatataa
      781 aatttaatga aaatatagaa ctaaatagga aaacgtagac gtaattttct acgttttcct
      841 tttcatatat attaaatatt acggaaaatt atataaacta atttagtgtt ttgtttgtaa
      901 ttcctaattt tgctccactt ttagataatt tttaggtcta tttaactcat cagacattct
      961 atgctcattt ttctccaaat ttgtttcaac tctttcgtac ttaattttgc gtatttgaaa
     1021 attcttcttt tagagtactt ttattacgat actagtatga tatatatcta tagcaaacag
     1081 ttaaaatctt cctaaaataa gcgaatagca ttcctaaaac ctataaaatt tttagatgaa
     1141 gcgacaagtg gacaattaat aaactacttg aacatgaaat tatggatcac ctttcaaatt
     1201 tagaaaatgg aattcttata cttattgcgc aacatttatt gattgcaaaa ttatgtgaca
     1261 aaaaaaacta gtagaagaaa gaataatggc ttgataataa agtactacct attaaaggta
     1321 gtactttatt taatttagta taaattaaca tactactata ttttaggttt catattatat
     1381 aaattaataa tatactacca tttgttggta gtataattta aaggtaataa gttattcttt
     1441 tattactatg tttgggtttt atagcaaaga aagtttaata agagtttgga cctagcaaac
     1501 aatattaatg aggtcattaa atcttattat ctttagtgat ataacgaaat atctacacag
     1561 acaaaatgat agcgtaaaat attttgtgta aatagatatt tagggtagaa aaaaagaaag
     1621 tccattctgt aaaattaaag ttaccacaac cgaaatttta taggaggact ttctcatgcc
     1681 caattttact acagaaatta tggaaacact aatcaataaa ggtgatttag atgaattatt
     1741 tcgatgtcat ttagaattag ctgttaattc attattgcag gctgaattaa cagcttttct
     1801 tgattatgaa aaatatgatc gtgctgggtt caattcaggt aattcccgta atgggaatta
     1861 ttcacgttca ttcagaacag agtatggaga attaaatttg gtaattccta gagatagaaa
     1921 tggtaagttt tctcaacaaa ctttacctgc ttataaaaga accaatgact ctttagaaac
     1981 cactattatt cagctatttc aaaaaggaat tactatgtct gaaatctctg aattgattga
     2041 aaaaatgtac gggcatcact atacgccaca aaccatttca aatatgacta aaatagtttc
     2101 tgaagatatt attgccttta aagaaaggtc cttagaatcc agatactctg tcatttttat
     2161 ggatgctact cacattcctt taaaaagaca aactgtttca aaagaagcag tttatattgt
     2221 aattggcatt cgattagatg gaaccaaaga ggttcttggg tttagtcttg ctccaactga
     2281 atctgcttat gtttggaaag agattcttca agacctaaaa gaccgtggtt taaaagaggt
     2341 tttattagtc gtaactgatg gtttaagtgg cattaacgat agtattcata gtgtttatcc
     2401 aaatgctcaa tttcaacaat gttgtgtcca tatatctaga aatatcgctc ataaggttcg
     2461 tgttagtgat cgacaagaaa tctgtagcga tttcaaatta gtttatcaag caagttcaaa
     2521 agaagaagca aataatcaaa taaggtttat gatagataaa tggaaaaagc agtatccacg
     2581 agtggttaaa ttgctcatga atcctgctat attaaccttt tataatttcc ctccgtcaat
     2641 cagaagaact atttattcaa ctaatttgat tgaaggtttt aacaaacaat taaagaaata
     2701 taccaaaaga aaagagcaat ttcctaatga agaatctctg gagagatttc tagtatctca
     2761 attcaacaac tataatcaaa agtttctatg ccgagttcat aaaggtttta aagaaataca
     2821 tgatacatta gaatcaatgt tttaacttga tcaacagaat gggcttttcc atttacacaa
     2881 aattattgac gctcccgaca aaattatgat cttgcgtaaa gttataaaat attaataaaa
     2941 atattttatt aatgaaaagc ttggtagcac ggttattcat atgctaattt atttcacttt
     3001 atagtaaggg gagttcttag tatattgttc aaacttaaat ttcatataat ttaattatgt
     3061 aaattagcta ttctaagaac ttctacggat aactgcccgt ctaaaattta aataagagct
     3121 tagaatcttc atataagtaa ggttaatttt tttgtacaaa aaagtcggaa tattctgagt
     3181 tactgaaatg atataaaatc ataaactaaa tcccttgatt ttattgactt tcaaaagttt
     3241 atatatataa gttaatgatt atggtaaaat atatacataa tcattaactc agaatatttt
     3301 taagaaggga aacaatagtg agctataatg tacaaccgtt aaggacaaat gaagagatac
     3361 aagattttca gttttggcta agaagaacaa gtaatccgga aagagatagt tttttatttt
     3421 tgtttggaat caataatggg ttacgtatgt cagatattat aggattaaaa gttggagata
     3481 tacgaggcaa aagtaagcct attattgttg aacataaaac aggtaagcga aaaccgattt
     3541 ttatagataa tttgagagaa gaaattttat tatatactga aggtaaagaa gaaaatgatt
     3601 ggttatttcc gagtcggcaa caaggtcgac atataacaag ggatcgtgtc tatcagatat
     3661 atgcagatat agcagaaaaa ttaggacgag atgatatagg aacacataca ttaagaaaaa
     3721 cttttgggta tcactactac aagaaaacta gagacattgc taccttaatg tttattttta
     3781 atcatagctc gcaagcaatt acaaaaaggt acattggtat aactgaagat gaaataggag
     3841 catcacttcg tggatttaaa ctaggtgtat aatttaaaaa tttttataaa cagtaaagac
     3901 aaagattaaa agctattttt atctagaacg attttatttc ataaagttcg cggaataaaa
     3961 gctggggtta ctggtacttg gtataggttc gtgttatata aactaagtaa ttaaataaca
     4021 tttttaggtt ggaaaaggat gaaattaaaa aaagagaatc aatacggtct caaaaacgca
     4081 aacgctgcgg aataagaaaa atactgataa ctcaacgttt agcagactat ctaataggga
     4141 tagtctgctt ttttcgtttt tgaccatact tttgaccttc tgcaaacttc cttgcttttc
     4201 ctctgtagaa ctcatgaaat ctgcaaaacg aattgccgtt tcctctgtct gctttttcgc
     4261 gatatgggtg tatatatcat ccatagtggt tttaatatct gtatgtccaa gtcgaacata
     4321 cacttctttg acagacgcac cagattcaaa gagcagacta caatgggtat gacgaaaacc
     4381 atgaagtgtg atatgtttta agttattctc tttaatgatg attttcaaat tgtgatttac
     4441 atagtctaaa taaaggggct tattttcttc tgatgaaaac aaaagttgtt caccatcttt
     4501 tataggtttg attctcattt ttagatatta tgcccgcacg tattcatcac atttctattg
     4561 tgaatcgctt tattcgaccg acatttgact tttatcataa tatactcggt ttaaaattac
     4621 taatgaaaac aattaatcaa gacgatcata ccatgtatca cctgtttttt tcagataatc
     4681 accaccgtgt gggaactgaa ctgacttttt ttgaagtaca agaagggaac aaccagtttt
     4741 ttggaaccaa tacgattgaa cgaacgatac taaaagttcc ttccgaagca tcattgcatt
     4801 tttgggaaat atattttgaa acacaaggcg tttgccatta tggaatcgaa tcattttctg
     4861 gacgacctat cttacgtttt gaagggccag atgcaacacg attagcttta gttcccttac
     4921 gcgaatttga agatattgat gattatttcc cgtatgtcac agaccagatc cctacagagc
     4981 atgccatttt aggaattgat gccattcaac tacgcgtaca atattccgat gcgactgaac
     5041 gagagttatt gtcggtaggc tggtagcaca aagaatacgt cagtttcttt tcgggtcatc
     5101 aacgagtgac aatcttagaa aatcaagaca ctcagttcta tcaagaagtt cagattattc
     5161 aagatcgtga gaatccagtt gccattgaag ggattggtag cgtccatcat gttgcgtttg
     5221 gcgtagaaaa taaatctgat ttacaacgca tcgacaaaca attacaagag cgcaatttta
     5281 taaattcagg aataaaagat cgtgaatttt ttgtatcact ttattatcgt gacccaaatc
     5341 aattattgat tgaaattgct acaggggaag gaaacctaga tgcaaaagcg tatgaaaatc
     5401 aaagtccacg ctttgaagaa attcctttat ttttaccgca gtacttaaat ttcattcgtg
     5461 aacaagtgga gcaataatta gctaatcaaa tccatgacga agagtaagag gttgactcat
     5521 aagtcaaaaa ataaagaaaa acgcatcaaa tccattctaa agaatgttga tttgatgcgg
     5581 ttcctatttt tataaactat cattttcgac taataaaact acttatgagt cagcctctct
     5641 ttttttcttg atttttatac ctctttctag gtaaactaaa catctgccaa aaataccgtg
     5701 tctcattgaa acattctcaa ctatttcaaa ataaagggga gtaccaaatg aacgagtata
     5761 ttgaaattat ccacgcaaga gaaacaactt aaaaatgtca gtctcaaaat tcccaaaaat
     5821 aaaattagta tttttaccag cgtatctggt tcaggaaaat catcgattgt ttttgatact
     5881 attacccaag aagctggtcg tcaactgaat gacacctatg atagttttac ccgtttattt
     5941 ttacctaaat acagtcgtcc agaggttgat gagattgata atctttctac ggcgattgtg
     6001 gtcaatcaaa agaagattgg tggcagtgcg cgctcaacgt tagggacgat tactaacatc
     6061 aatgcgttat ttcgtgtgtt gttttcatgt tttgcaacac ctagtttagg atatgcaaat
     6121 gcttattcat ttaatgatcc tagtggaatg tgttctgtct gtcagggaat cggtaagacg
     6181 attgcatttt taataggatt gctttatttt tcggctattg ataatgctga ttttgtattt
     6241 tctgatccat ttaccgcaga agttttgtct ggaattgagg aacaggcaaa gaatcatggt
     6301 tattttgtat tggtgcataa tgttaattca gcagaagatg ttaaaactat tcagagaaat
     6361 tggcgttttg aggggttcat tgttgttggt gtacgtgtaa aagaattttt ggaattaaat
     6421 aacgtttttg atggtcctgt tgtttatatt gatacgcatc tttcaaaaga gacattaaga
     6481 gaacttgaac aacaaatgaa tcgtttattt gtaaatacaa atgattacca agctagtaca
     6541 ttggcagtgg aacatttgat tcattatggt catgagaaaa tagcgtttct ttcttttgac
     6601 tacattgtag gtgaaccaag tgttattcaa gaacgacatc aagcttatat cgatacatta
     6661 aagaaatatc atttaacctt taaatcgcat tggatatata acaactctga atttgagcga
     6721 atttatcaag aattagctga atttactgca attgttgtta ctggagatta tttagcggct
     6781 aaattagtaa aatttttaaa aaataagaat aaacgagtga tggaacaatt atcgattatt
     6841 ggatttgatg atattacttt tgcagaatta atggatcctg cgttgacaac aatacgacta
     6901 aatccaggta acaagggggc gattgctttt caccaactga ttggactggc caaacaagaa
     6961 gaaataaaaa atcaagtact actattagag ggagaattga tttctcgaga ctctgttcac
     7021 aaaatcaaat aaaaaagctc tgtgaaataa gtatagttaa caataaaata tccgaacgat
     7081 agagagaatg aactgtgatc tagcagttcc tcgtcgcaaa ttcactagaa ttagcattat
     7141 ttactctaaa ttgttcggat attttttctt gtttttaatt atgattacag tgcctatttt
     7201 taacgaaatt tgcttatttt caaaatgtat gattatatgt tagtaaacac tttcaaatat
     7261 gaaaagagga gctattatgg taggaattag ggatgttgca aaacgggcgg gtgtgtccat
     7321 tgcaacagta tcgcgcgtgc taaatgagga tccgacactt tcagtaaccg atgaaaccaa
     7381 acgaaagatt gcagaatcgg ttaattttta tcattatgtc aaaaaaagtc ccgttgcaaa
     7441 aaatttaaca aatattgcat taataacgac agtttctgaa gtagatgagt tggaagatcc
     7501 ctattttcga acgattcgca gagggattca agtcgaagca gaacaaagca aagtcaattt
     7561 gaagaaaatt attcgtttgt cagaaacacc gttggagcaa gacgatttgc taaaatgtca
     7621 aggtgtgttg attattgggc aggtgctatc ttctgtgatt gaagaagtat cagcaattaa
     7681 ttcgaatgtc gtagtgattg atgaccctag tattgaagct gaagtagatg cagtttatac
     7741 tgatttaaca aaagcaacga atcagcattt agatcgattg tataaaaaag gacaccgaaa
     7801 cattgcgttt atcggaggag gacgtgttcg tttagatagc caaggaaata aacacgtgtc
     7861 agatgatgat tatcgaaaaa tagcctatga gacgtggatg aaacaaaaag gattagaaaa
     7921 atatagtcaa atttatttag aaggctggac cacacttgac ggaatgcagg cagcagattt
     7981 acttttgaaa aaacatgcta aagataagtt gccgactgct atcatggtag gaaatgatcc
     8041 tattgctgtg ggggtttatc gttcacttca aaaaaatggt ctacatattc cagaagatat
     8101 ttcgattgtg agttttgata atattgaagt tgctgaattt ttaacacctg cattaaatac
     8161 ggtgaatgtg aatacagaag aaattggaaa actagcagtt cgcatggtta atgaacgaat
     8221 aaaagaagtt agaaatgttt cgatcaaagt gattgtttct aataatatta ttgttagaga
     8281 aagtgaaaaa aatatcgatt aaaaaagtaa acatccaaaa aagggtgttt acttttttgt
     8341 ttactaatat ttttagtaaa aaatctcgac aaaacattaa gtaaacagtt atggtgttgt
     8401 taacaaaaag aaaacgcatt caaaacagga ggaattaaga tgaaacattt caaaaaaata
     8461 gctttaacag ggatagctgt tttaactggg acattactcg cagcatgtgg tgggggagat
     8521 agtaattcta aagacgggca agtaacttta agctatgcga gttgagatac tgaccaagct
     8581 ccaggactaa gaaaaatgtt agatgaattt gaaaaacaaa atcctgatat caaagtagaa
     8641 atggagacaa cgccttggga tcaatattgg gttaaattag aagctgcaac aacgggaggt
     8701 aatatgccag atgtagtaac catgcactct agcgaatcct ataaatatat gtcacagaat
     8761 gctttgatgg gacttgatga tgttatcaaa gaaaatgata ttgacatggg aaactttact
     8821 gaaggaattg ctgatttcta tacgcttgaa gacgaactat acgcaatccc taaagatgct
     8881 tctgtagtgg ggctatggta caacaaagaa ttatttgata atgcaggtgt cgattatcca
     8941 gatgaaacat ggacatggga aacactcaga caagctgcaa ttgatttaac agatgaatca
     9001 aaaggcatct atggttttgc atcacatatt ggaacagaga ctggctactg gccatttatt
     9061 tatcaaaatg ggggagaagt atttgccgat gacaatacaa aatcaggttt gaatacccca
     9121 gaagcacaag aagctttagc tttttatcga gatattgcac ttgttgatca tgcttcacca
     9181 acaattacag aattacaaga tcctgataag ttaagtcgtt ttcaatcagg tagattagca
     9241 atgatgattg aaggaaactg gtttgcaccg ggatttttga aaaatgacta tctagttgaa
     9301 aatgttgctt ctgccgtgtt acctaaggga aaagttaatg caacaattac aaatggactt
     9361 ggttgggctg ccagtgcgag tacaaaatac ccagaagaag tgaaaaaact aatggcattt
     9421 ttagctagtg aggacgccaa taaaatccaa gcggaaacgg gggcatcaat cccagcacta
     9481 aaaggctttg aagacgagtg ggctgcttct tcagatacaa ttgatttgag tgcttttgga
     9541 aaaatgttgg agtatggagt ccctcgtcca tacaataaag atggattacg agctgaacaa
     9601 gcagaagttc aaattattaa ccgtattctg tctggtgaag taactgttga agatggaacc
     9661 gctcaaattg aaaaagaagt caatgcgata ttgagcggaa attaaattag ttaattatac
     9721 aatatacagg cgaaaggatt ctatttcgtc tgtatattaa tggatgaata ccaaaaaggg
     9781 ggatattatg aaaaatcgaa tttttggaaa atatcgtaaa gatcaagtga tatggggcta
     9841 tatttttatt tttccaacgg cattaggatt aattttatta aatatatggc cagcaattca
     9901 aacagcaatt ttaagttttc aaaaaacggt agggttcggt gatactgaat gggtcggatt
     9961 aagaaattac caaaaaatgt ttgcagacgg tgaagttttt cagtctttat taaacacatt
    10021 aatttatgca gtggtttcga tacctagtat tgttgtatta tccttgttgg tggcagtttt
    10081 aatgaataga aaagttaaag gattgtcgat ttatcggaca atttattttc taccagtcgt
    10141 tgctgcacca tccgcagtcg caatgatttg gcgctggatg ttcaataatg attatggact
    10201 gattaataac atgttggcta aaataggtct cgatggacca gcatggttaa cggatccatc
    10261 gattgcgatt tattctatta tcattgtagg agtttggagt gctattggtt ataacatggt
    10321 gttattacta gccggcttat aagaaattcc taaagattat tatgaagcag cggaaattga
    10381 tggtgctagt ccaatccagc agtttttcaa cattacgtta ccattagtta caccaacatt
    10441 attttttgta gttgtaacaa cagttatcaa tgctttccaa gtatttgatg ttatttttat
    10501 gatgattact cctaatagta ccgccatgtt taaatcacaa tcattagcct atttattcta
    10561 taaacattca tttattttaa atgacaaggg atatggttcg gcaatcgtta tgttcctttt
    10621 agtgattata ttaattatta ctgcaattca aatgaagttg cagaaaaagt gggtaaacta
    10681 ttaaggaggc tatcaaatta tgaaaaataa atttttttca aatgcgccaa tccatatcat
    10741 gcttatttta ggtggtattg caatggcatt accttttatt tggatgattt taacttcagg
    10801 gaaaacgttg gttgaatcta cccaaattcc acctacaatt ttccctgaga aatttcagct
    10861 aagtaactat caacaagtac ttgatttatt accattcatg gctttttata tcaatacatt
    10921 tttaatggtt gttggacgag tgattggctc ggttttattt tcagcaatgg ctgcatatgc
    10981 ttgtgcgcgt ctaagttttc cagggaaaaa tatccttttc agcttagtac ttattcaaat
    11041 gatgattccc ggttccattt ttattactcc acagtattta ttagcacaaa aattgggtat
    11101 tttaaataca attccagcat taatattacc aggggtagtt agtgcgtttg gtacgttttt
    11161 attaagacaa tttttcttag gtttacctga tgaattggag gaagcggcct atttagaagg
    11221 tgccaatatt tggacgatct ttttcaaaat tatgttgccg cttgctaaat caggattggt
    11281 tgcacttgga atattcacag cgttgtttgc atttaaagat ttaatgtggc cattgattgt
    11341 caatacttca attgataaga tgcctttagc tccgggatta gcaaacttac aaggacaata
    11401 tgccacgaac ttcccacaat taatggcagg ttcgttatta gcaatttggc caatagttgt
    11461 gatattcatc gttttccaaa gaaaattcat tgaaggaatt gctacaacag gtagtaaatt
    11521 ataaaactat taattagtga ttggagagta agtacatgac aaaaattaca tttattggtg
    11581 caggaagtac tatttttgca aaaaacgtat taggcgatgc gatgttaact ccaagtctac
    11641 aagatgcaca tattgcattg tacgatattg atgaaaaacg actaaaagaa tcaaatttaa
    11701 tgcttaaaaa tattaataaa aattcgaatg aaaatcgtgc tgatattgtt tcttattatg
    11761 atcgcaaaga agcgttaaga gaggctgatt ttgttatcaa tgctattcaa gtgggtggtt
    11821 ataaaccttc aacagtgatt gattttgata ttcctaaaaa atatgggttg cgacaaacta
    11881 ttggtgacac catggggatt ggtgggattt ttagaaccct acggacacta ccagtaatgt
    11941 tagactttgc taaagaaatg gaagaagttt gtccaaatgc ttggttattg aactatacaa
    12001 atccgatggc gatgttaacc atgggtgttt taaaagcgac gagtattaag actgttggtt
    12061 tatgtcatag cgttcaagta tgcgtaccag aattgtttga gcatttagga atagccgatc
    12121 aatatgactt aaatgacttt caatggaaaa ttgcaggcat taatcatatg gcattcttgt
    12181 tggaaattac aaaaaatggg gaagatttct atccaacaat tcgtcagtta gctgcagaaa
    12241 aaccaaatcc acatccagat tctgtacgtt ttgaattgtt gaaacgtttt ggttactata
    12301 ttacagaatc aagtgaacac aatgcagaat accatccata ctttattaaa tcaaaatacc
    12361 cagaattgat tgacgaatta gggattccgt tggatgaata cttacgtcgt tgtgaacaac
    12421 aaattaatga ttgggaaagc atgcgagagg acattgtaaa taatggggag ttaacacata
    12481 cgagaagccg tgaatatgct tcatatatca tggattctat tacgacagga actccgtcag
    12541 tgattgcagg aaatgtctta aataaaggct taatcacaaa cttaccgaca gatgtttgtg
    12601 tggaagtacc ttgtttagtc gataaaaatg gcgtgcaacc aacctatgtc ggtgaattgc
    12661 caacacaatt agctgcttta aatcggacga atatcaatgt tcaagaactg acagtagaag
    12721 ccgctttaac actagataaa tcaaaaattt atcaagcagc gtatttagat ccacatctat
    12781 catctgaatt gtctttaaca gatatcaaaa atttagtgga tgatttaatc gaagcacatg
    12841 gggactattt acctgaatat aattaaaaag aagctgaaag agtaaggaat tttcaattcc
    12901 ttactctttt cttagttaaa tttagtaaaa atattgacaa aacacttagt aaacagttac
    12961 attagtgtta acaaaaagaa aacgcattca aattgtataa ggaggagcat aaaatgaagt
    13021 ggtctaaaaa aattatttta gcttgtatag cactaggagc aacgatctct ttgactgctt
    13081 gtggaaaaaa tcaggaagat gcaagcggta aggtagaaat agagtttttt aatcaaaaaa
    13141 aagaaatgac tcaaacgatt caggagattg ctaaagattt tgaagcaaaa aaccctgata
    13201 ttcatgtaaa agttgtggat gtaccgaatg ctggtgaagt aattaaaaca cgtatgcttg
    13261 caggcgatgt accagatgtc attaatcttt atcctcaaag tatagaattg aaagaatggg
    13321 cgaaagctgg ttatttagag gacttaaccg aagaaccgta tttagagaat atcaaaaatg
    13381 gatatgcaca acgttttgct attgaggatc gtgtatatag cataccatta accgctaatg
    13441 tctatggttt ttactataac aaaactgctt ttgaggaaat ggggattcaa gcaccagaaa
    13501 cttgggcaga atttgaaaaa atagtagctg agattaaaga tcaaaacaaa gtaccgtttg
    13561 cgattgcagg agcagaaggc tggactttaa atggttatca ccaactagct atagcgactg
    13621 ctgctggtgg tgaagaggaa gcgaacaatg tttggcgttt ctcagaagtt aatggaatta
    13681 atgctgaaag caaagaaatg caaaaagatt ttaatcgatt agatttgtta agagaaccaa
    13741 aagctttgca aaacaactgg caaggtgccg gttataacga tacagtagtt acttttacaa
    13801 aaggtgatgc gttgattatg ccgaatggtt catgggcaat gccgatgatt cattcacaaa
    13861 atccagattt tgaagtagga acttttcctt ttccagcaga tgaagcaggt caaagtctaa
    13921 cgattggcgc aggagaccta gccttatcag tttctgcaac ttctcaacat aaagaagaag
    13981 ccaaacgttt tgtagaatat atgacaacac ctgaagcgat gcagaaatat tatgatgtag
    14041 atggtgcacc gtgtgctgtt gagggcgtta tcgagaatac tcaggattca ccattaagtg
    14101 gtttaactga acttgctttt actgatcgtc atttagtttg gttagccaaa gattggaata
    14161 gtgaaaatga tttttatacc ttaacaacaa attatttaca caatggcaat caaaaagcca
    14221 tgattaacgc attaaatgct ttctttaatc caatgaaagc tgacggaagg taggaaaata
    14281 aaatgaaaat aaaagaaatt ttaaatcgtt actgggcaat catttttgta gcaattccaa
    14341 ttattttgca gatgattttt ttctactttc caatggttca aggtgctttt tacagtttaa
    14401 ctaattggac tggtctgact tataattttg attttgtggg tttgaacaat tataagattc
    14461 ttttttctga tccgaagttt atcaaatcga ttggcttcac tttaattgtg acgctttgca
    14521 tgattgctgg acaaattggt tttggtattt tgattgcgcg ggcgttgaac gcaaatatta
    14581 aagggaaaac attctttaga gcatggttct tttttccagc ggtcttatcg ggcgtggttg
    14641 tttctttagt atttaaacaa atttttaact atggtttacc aaaaattggt agtatgttag
    14701 gcattgaatt tttacaaacc agtcttttag gaacaacatg gggagcgatt tttgcagtaa
    14761 tttttgtttt actttggcaa ggaattgcca tgccagtcat cattttctta gcgggttttc
    14821 aaagtattcc acaagaaatt attgaagctg ctagaatgga tggggcgaat gataagcaaa
    14881 ttttttggaa tattgaaatc acctacttat taccaagtat ttcaatggta tttattatgg
    14941 cattaaaagg tggcttaaca gcatttgatc aaatttttgc tttaactggt ggtggaccaa
    15001 gcgatgccac aacatctttg gggttacttg tttataatta tgcttttaaa aacaatcagt
    15061 ttggttatgc gaatgctatt gctttagtct tatttatctt aattgggatt gtttcactgg
    15121 cacaattaaa attatcgaaa aaatttgaaa tctaggaggt ttcccaatga aaacatcaaa
    15181 agaaaaaaat atttctaaat acttgctttt attattaggg ggcttcatga ttttagttcc
    15241 cttagtttca acgctattcg gttcatttaa aacaacaccc gatattatga ataatttctt
    15301 tcgttttcct aatccagcga ctttggaaaa tttccgaaga ttgttagcgg atggaattgg
    15361 tcattacttt tggaattcgg caatcatcac aatcattgca gtagttgctg tgacagcaat
    15421 tattcctatg gctgcttatt cactagcaag aaaaatgact cgtgggaaag catacggatt
    15481 gatgtattta tttttaattt taggaatttt tgttcctttc caagtaatta tgattccgat
    15541 taccgttatg atgagtaaaa ttggtttagc aaatatgtgg ggattaatct tactctatct
    15601 cacttatgcc gtaccacaga cactattttt atatgttggt tttattaaac taaatgtgcc
    15661 tatcagtctg gatgaggctg ctgaaattga tggggctagt cactttacaa cttatttcaa
    15721 aattgttttc ccaatgttaa aaccaatgca tgcgacgact attattctta atgctctatg
    15781 gttttggaat gatttcatgc taccattatt aattttaaat cgtgattcta atatgtggac
    15841 actaccattg tttcaatata attatacggg acaatatttt aacgactatg gtccaagttt
    15901 tgcttcctat acagtaggaa ttgtcacgat tacactagtt tatcttgctt tccaaagaag
    15961 tattatagct ggaatgagca atggagcagt gaaataacca ataaattatt ctcattataa
    16021 aggagttcat aaacttatga ttatatttga caatcacaca caaacatttc atttacaaaa
    16081 taaaaaaatt agctatctat taggaatcga agaaaataat tatttaacgc atctttattt
    16141 tggaaaaaag attacgcact attctggtgg gtatcgctac ccccgaacag atcgttcctt
    16201 ctcacccaac aatgcaaatg cgcaagatcg tttattatct ttagacacat taccattaga
    16261 atttccagga tatggacatg gtgattttag agaaccggcg cataatatta aattaggaaa
    16321 cggtagtcgt gtaaatgatt ttcgttatga tagctacgaa attcaaaaag gaaagcccag
    16381 cttagcggga ttgccagcct tatatacgga agatgatgat gaagctgaaa cgctgattat
    16441 tcaattgaaa gacgcgatta gtggattgac tttacagttg ttctacacaa tttatcaaaa
    16501 tcacagtgta attattcgtt ctacgcaatt agtaaataat agtgatgaaa cagtggagat
    16561 taaccgctta gctagtcaat caattgattt tccaaatcgt ccactcgagt taattcattt
    16621 aaatggtacc tgggcaagag aacgtcaaat gactagagaa aaaattcata cagggataaa
    16681 agtattagat agtaaacgcg gatcaagcag ccatcaacaa aatccgtttg ttgctttagt
    16741 agatgccaaa acagatgaat ttcaaggaga ggcatatgga tttaatttag tttatagcgg
    16801 taatcacgaa acggtgattc aaaaagatcc atttgaccaa acacgggtca ttatgggaat
    16861 taattcattt aattttggtt ggcaactaaa aactggcgac tcatttcaaa cgccagaagt
    16921 cgtgatggtt tatagtaatg aaggtctgaa tacgttatca cagacgtatc atgaattgta
    16981 taatcaccgt ttaattcgtg gaaactatcg tttaaaagaa cgtcccacgt taatcaacaa
    17041 ctgggaagca acgtattttg attttaacga agaaaaaatc atgaatattg tcgatgatgc
    17101 gaagaacttg ggcattgaaa tgtttgtttt agatgatggt tggtttggcg gtcgtgaaaa
    17161 tgacttcact tcattaggtg attgggttga aacagaaggc aaacttgctt gtggtttgga
    17221 aaatttagcc aagcaagtgc atgataacgg gatgaaattt gggatttggt ttgaaccgga
    17281 aatgatttct gaagatagtg aactatttcg agcacatcca gattgggcat tgggtattcc
    17341 aggtcgtgac cgctcaatta gtcgaaatca atatgttctt gatttttcaa gacaagatgt
    17401 tcgtgataat atctatcaac aaatgacggc gatttttgat cgtgtaccga ttgattatgt
    17461 gaagtgggat atgaatcgaa atatgacaga agtctattct gtcttgttgg actcaacgat
    17521 gcaaggtgaa gtggctcatc gttatatgtt agggttatat gaatttttag aaaagttaac
    17581 aacgaattat ccaactattt tatttgaaag ttgttcgggt ggaggcggtc gttttgatgc
    17641 aggtttcttg tattacatgc cacaaacttg gacgagcgac aataccgatg ccgtggctcg
    17701 tctgaaaatt caatatggaa ccagcttagt gtatccaatt tcaagtatgg gtgcgcatgt
    17761 ttcagcagtc ccaaatcatc aaacaggaag agaaacgagt ttaactattc gtggcaatgc
    17821 tgcgatggct ggtgtttttg gttatgagct agatctaacg caattaactt ctgaagaaaa
    17881 agcagaaatt aaagaacaag ttgcctttta taaaaagcat cgccaacttt tgcaatatgg
    17941 aactttttat cgcttggaaa gtccgtttga aggcaatgat actgcatgga tgtttgtctc
    18001 aaaagaccaa acagaagcca tggtctttta tttccgagta ttagcagaag ctgcgcatcc
    18061 attagtcaca ttgaaattag caggattagc agaagaagca acttatcaaa tggaagatgt
    18121 aacatttgct ggcgatgaat tgatgaatct cggtttttat attgactcag aactacatgg
    18181 tgattatgcg acacaacgct tttatttgaa acagatatag tagcttgaat ttacaatata
    18241 agtgcaacac caaaagagac tcattccaaa cgaagaaaat atgttttatt tacacgcatt
    18301 tactaaaaag cagcctgatt tgaattggga aaatgaagaa gtacgtgaag aaatctatgc
    18361 aatgattaac tattggttag ataaaggact aggcggattt agaatcgatg cgatattaaa
    18421 catcaaaaaa aaactagagt atggcatctt tgaatctgat ggagaagatg ggttagcctt
    18481 cattgggccc tggattctta atcagtcggg cattgaagtg tggttaaaag aaatgagaga
    18541 gcggacattt aaaccacata atagtatgac ggtagcagaa gccgatgttc ctaatgaacg
    18601 tttagatgaa tatattggtg aagatggcta ttattcgatg gtttttgatt ttagttatac
    18661 ggatattgat gtacctgaaa ctggtgaatg gtttaaagat agccaatgga catggacgga
    18721 tatgcgtacc aatattttta cgaatcaatt agttacgcaa gataaaggtt ggggcgcctt
    18781 atatttagaa aaccacgatc aaccacgttc aattaataaa tatattccag aagaatggat
    18841 caatgattat agtaaaaaaa tgttggcgac gctttttatg ttactaagag gtaccccttt
    18901 tatttatcaa ggacaagaac taggaatgac caacatagaa atggcatcat tagaagattt
    18961 tgatgatgta gctacgcatt ctcagtataa acgggcatta gaatatggat tggcaccaga
    19021 gcaagcattg aaagctgtca ataaacgaag tcgggataat tctcgcacac ctatgcaatg
    19081 gaatacgcaa aaaaatgcca gattctcaac ttctgataat gtatggttaa aagtaaatcc
    19141 taattatcca gaactcaatg cagaagctca gttaaccaat caacagtcgg tattcaatta
    19201 ttatcatcaa ttaattaaat taagaaaaaa tagtgtatac aaagacgtgc taatctacgg
    19261 acaatttcta ccagtgaaag ataaaaatga acatattttg ctttatgaac gccaattaga
    19321 agataaaatc gtattagtta tcttaaacgt tacagcaaca gaacaagaat attatgtaga
    19381 tgaacagtac caacaagtat taatcaataa ttatgaagat gttttattag atcaaagtcg
    19441 aaaattgcgt ttacgaccgt ttgaaagtat tgtattagct aattatggag gacaattatg
    19501 aaaattaaaa acgaagcaat gctcatcacc tatgcagata gtttaggaaa aaacatgggc
    19561 gaattaaaac aagtcttaga caaacattta caaggtgtta ttggtggtgt acatctgttg
    19621 cctttttttc catcgacagg tgatcgaggc tttgcgccga gtgattatac gacagttgat
    19681 ccagctcttg gaacatggga agatgtggaa gcattaggtg aagattatta tttgatgttt
    19741 gattttatga ttaatcacat ttctcgtgaa tcaaaatttt tccaagattt caaaaaaaac
    19801 catgagcagt caccatacaa agaaatgttt atccgtattc atgaattttt cccagaaaat
    19861 cgaccaacac aagaagatat tgatttaatc tacaagcgta aagacaaagc gccgtttcaa
    19921 acggtagaat ttgcggatgg tacgacagaa gaggtttgga atacctttgg ggaagaacaa
    19981 attgatttag atgtgaccaa agaagttgtg aaagaattta ttcgagaaac catcaaagat
    20041 atggcgaacc atggctgttc tttaattcga ttagatgcgt ttgcttatgc gattaaaaaa
    20101 ttagatacca atgatttctt cgtggaacca gatatttggg aacttttaga cgaagtgcgt
    20161 caagaagcag cgaaatatga cgtcgaatta ttaccagaaa tccatgagca ttattccatt
    20221 caaatgaaaa ttgccaatca tgattattat atctatgact tcgccttgcc aatggtcact
    20281 ctgtattcac tttatagtgg aaaatcagaa cgtttagcca attggttgaa aatgagtccg
    20341 atgaagcagt ttacaacatt ggatacgcat gatggaattg gtgttgtgga cgctcgtgat
    20401 ttattaacag atgaagaatt ggattacacc tctgaggaat tgtataaagt cggggcgaat
    20461 gtgaagaagg tctattcaag tgccaattac aataatttag atatttatca aattaacagc
    20521 acctattata gtgcattagg cgataatgac cgtagctatc tgttagcacg tgcgattcaa
    20581 gtatttgcgc cagggattcc acaaatttat tacgttgggt tattagcagg aaaaaatgat
    20641 attgaactgt tggaagcaac gaaagaaggg cgtaatatta atcgtcatta ctatgattta
    20701 gccgaaattg aagaagaagt gcaacggcca gtgattcaac aattatttgc acttttaaaa
    20761 tttagaaatg aatctgcggc gtttgatttg gatggaacga ttgaagtagc aacgccatca
    20821 gaatcagaaa tagtgattac acgtaaaaat aaaaacggta gtgatagtgc aacattaacg
    20881 gctaacttgg atacgaaaga atttatcatt gagtctaaag atataaaaat tttcactagt
    20941 gttgcattaa aattttaacc tattccatag aacaaaaaat cctttatact agcacttgga
    21001 tgacttatcc aagaccaata aaaaggactc atacatggat aagtataaaa caaatacagc
    21061 atttaataaa tggttttctg ccataaaatt aggaaaactt tctgtcaact cccaaaaatg
    21121 tatttttgat tacaattcgt atcgtaaaaa actgtctttt gaagctgtgc tcaagctctt
    21181 tctctacgca gttaatgaag aaaaagaaag tttgcgtgat ttgagtacag ctcttatcga
    21241 ccgatcgctc caactagaaa ccgatgtgtc atcgattagt catacccaat tgtctcgagc
    21301 atttaatgca cttgaaccta aagtgttaga agaaattttt caacaactgt tagaaaaagt
    21361 tcaacatcaa atcaaaccaa ccaagcgaaa tcatctgtat ctgattgatt ccagtacgtt
    21421 ttcgcttagt ttaaaacgtc ataagtgggc gactttccgt caaacaaaat caggtgtcaa
    21481 gttacactta aactattgtt atatggacga tactacgatg tatccaactg attttacctt
    21541 aacaaatgcc catgaacatg atctcaatca actaccagta ttggtgaatc aaccagaggc
    21601 aacttacgtg tttgatcgtg ggtattagga ctttgaaaag atggatcaga tgcattggga
    21661 tggctatttc ttcgtcactc ggattaaaaa aaacaccaaa gttcacgtga tagatactct
    21721 ggaaacatcg ccagaaacag aaattctacg agacgagctg gttcgtttag gatctaaaac
    21781 ctatctaacc gccaatttcc gactagtcac tgttcaagat aagaacggac gtgtgtttca
    21841 atttatcacc aatcgaatgg acgtctcttc aaaagaaatc tctgatatgt atcacgctcg
    21901 ttggcaaatc gaattgtttt tcaagcacat caaacaacac atgaccatta aaaccttctt
    21961 ttctcaaagt gaaaaaggcg tgcagaatca attgatttta acgatgattt ccgctctcct
    22021 cacgtttctc atcaaattgg agacgaaaac ggaaaaatca gtgttccaaa tcaaacgatt
    22081 ctttcgttat ttactgtttc agccctttga atgttgtcta gaaaaattaa ttcccacatg
    22141 agaaaaaaga aatttggctc gttacttttg gttttcagtt gaataacaga taaaaaattt
    22201 tctggaaaaa gtcatcctct gcgtcagcac agtttttttt gacttatttt cagaggaaat
    22261 agttttcaga atattctaaa atgttttatg caacactagt gatatttttt agaattagat
    22321 ttctaatgtg cattttttga gtattaagga gcaagtatac aaagatttat aaagctgatt
    22381 aaatttgaat aaaaatatgc aggaacgcta ctatttttag tagtgttctt ttttatattt
    22441 gaataaaaaa tgtggtggtt aaatggtggt tataacaaaa aatgaattaa tgaaattagg
    22501 ttatgggaag tatcagtctg agagaatcat acgacaggca aaagcaatta tggtttcaaa
    22561 aagctatacc tattacgaaa atagaagatt aggactagta cctgttgaat ctgtagaaga
    22621 agttttaggt atgaagatag aggtagagga ggattttcag aatcatggtt aattttaaaa
    22681 aagataaaaa tgggaaatat cattttactg ctagtttggg attcgacaaa cgaacaggga
    22741 aaagagtaca aaaaaacgta gaggggttaa aacagttaaa gaagaaaaaa agcgtatacc
    22801 gaacttgttt ctaattatgg aaaagaagca gaaacttctc aaagtactat gttgtttgag
    22861 gagttttttt ataaattatt cctaccttat tatcaaggga aagtagaaga aagaacatat
    22921 aaaaatcggt tatctacaat caaaaaaact tttcttattt ctttaaaatg gagttaagag
    22981 aaatacgtcc aattgacatt caaaagtggc aaaataattt attgagcaaa tataaaaatt
    23041 cttatgtaag aaacatgtat gggatttttc agatgtcatt agatcgagca gttgttttgg
    23101 gactacttga aaaaaatcct gcaaaagtta ttggaaatgt gaagaaaagg aaaactacag
    23161 ttgatttttg gacaaaagaa gaatttacaa aagttatttc aactttttat attgatgatt
    23221 tgtaccaaca ttttggtttt atttgtatgt ggttactttt tatgaccgtt atgcgtttgg
    23281 gagaagccac tgcattaact tggaaagata ttgattttga aaataaaaac ttgcgtgtga
    23341 ataaaacact ctattataag agagccaaaa cctatgagtt tgtagaaccc gaaacggctt
    23401 ctagtattcg tacaatcgaa ctagacgaca atacaattac ctttttaaaa gcttggaagg
    23461 agcgtcaagt ttcaatagga ggcatgtcgt ttgtactttc ttataatgga acacctactc
    23521 agaaatatac aatccgtcat attatgagaa gacatgcaac gatcgttggt gttcatataa
    23581 tccgcataca cggattaagg catagtcatg cttctttgct tatatcacaa taaaaagatg
    23641 cacttcgaat taaagaaagg ttaaaacatg ctgatgtaca aacgacttta ggaacttatg
    23701 gtcatttgta tctaaattca ttgagagaga taacaaatga tcccacagat tatatccaaa
    23761 ctaatttttc taaaatcaaa ccgacaccta tttacgataa taaaaaataa atgataaaca
    23821 aaagtaggat cacaccaaaa gaaacacact agaaaaacta taatacaggc ttttctagcg
    23881 tgttgtttct tatttccgcc tatttataca ttatactcat aaagcattta atgcatcgat
    23941 cgtagccgca ttgtctatat gggtatataa agttgttgta ttggtacttg aatgtcccaa
    24001 ttgttgggca gttaatacca atgaatttgt ctgttggtag agtctagtag ctaatgtatg
    24061 acgcagtttg tgagggctga cttgaattct aaagcctttt gaatattttg ctaccatccg
    24121 ttcgattgca ataccggata tacgcttggc ttctccttgg taaattgcta gaaataaagc
    24181 tttttcgttt tctgttactt tatatcgttc tttccgaata gctagataat tagctaaata
    24241 ttccattgcg aatgctgcta tattgacagc atctctttta tttccttttc tagttactac
    24301 cacattattc ttatctaaag ataggtcatc taaattaata tttataacct cggagagtct
    24361 caatccagat cctaaaatca gtgcattgat cgccaggtct ctttctacat ctcgttgata
    24421 atagatcaaa gctcgtttac tcaaagtatt tttatattca ttggcaatat aatctagata
    24481 attttgtgta tccttatcta gaaataattt tttctcaatg gcattggcac gtgcagctaa
    24541 cgtagtttta tctgtgatgg ttgctacttg atccatagga ttattgtcta agtatggttt
    24601 tcctagagga ttattggaac ttttcgaaag atatttaaat agaaccttta atgccgtgat
    24661 atttctattg atagtagaag tagatagacc taagttatta ttatctttga gcttagtttt
    24721 tgttgtctct ttttttacat tttcttttgt gcgacgtttc aaatgagatt tgtacaagtt
    24781 taggtcatag gcagttaact gtcttatcga ggtgatagaa gtatcaggaa cgatagactc
    24841 actcagaagc cattcataaa agagcttata attttttaga tattctaaag tggttcgagg
    24901 tgattgatct ttatcctcac gatcttgaac aaaatctttt atataatcgg gcatatgaag
    24961 tatgattgta tctatttttt gatatatttc tgtatttttc atgtcattca tcctttcaat
    25021 aagtagggga gaagagtatc tctataattg gactatacca attatagaat gttttgtcaa
    25081 ttggtatgct ctaaaattta ttaatttgtt tcaaaaaaag catataaaca ggtatacgtt
    25141 taataattta aaataaatgg tatacataaa tagggaagga aatagaaggg agcaatctaa
    25201 attttttgaa ttttcttaat taattatata gaaagaacaa tctttctata gagttagaca
    25261 tgagagggac tatgcttgat ttaaaatcaa caagcgtata tactgtatat acaaagaaaa
    25321 aggagggaga agactatggc aattaaagaa agaaaattta gggaagtggg gaactcagta
    25381 gtattaacta tttcaaagga gtttcttaaa gaaaatgggt taaatcccgg agatacggtt
    25441 tttttagatg aagaaaaact tcgagcggca attacaaaaa aacaagcctt atctgatgaa
    25501 attgatttag caatatctca agcattaact gaatatgata ctacttttag agagcttgtc
    25561 gataaatgag tattcattac ctagaagcca aagatattat aaaaatgaat gtaatccaaa
    25621 taaaaaaata ctctcctaat gaaccccttg gtgtaaagga tgcgaatgct ttagaaatgt
    25681 gtgtagggca acttcgagcc tcagcttttg gtgaagaggt ttatccttcg atttatgaaa
    25741 aagcagctat cctacttata caattgataa agaaacatcc ttttcacaat gcaaataaac
    25801 gtactgcttt cttagctact tttgtctttt tgaaaatcaa tggacagctt ttagctattg
    25861 atcaaaaaga tgctgtaaat ttagcggtat atattgctac gtatgataag gatttcgatc
    25921 aactaaaata tgaagtgatc aacactataa agacacatac gcagcccatt taaggagtta
    25981 ctgtatgtgt ctttataggg ttattagctg ctatagcgaa ataattgaag taaattacaa
    26041 atagctataa ttcaaatcca actatataga aaacgtaaat tttctatata gttggattaa
    26101 gataacgact atttatttta tcatattttt actataccct atacttttac ttggtatatt
    26161 tatgaataat cgcaaattta cccctatact actagtttgt aagttagatt aataaaaata
    26221 ctaaagttat ttgttctttt attaaataca cctacaactt ataaattgat ggtatttaga
    26281 taacacgaaa gaataaaaat aacagctgat agtaaagttt gttgacgatt gctactcaat
    26341 cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact tatgaaatga aagacctttt
    26401 acatcttgca gaaatcggta ctgggattaa actaagcaat aaaaactaat gttcccctca
    26461 ttctttgaac ttctactatt tgatttattt attctgataa atgacgattt caccttcagg
    26521 atcttcaaat cccgtataga atttatttaa gatctcttca gtatcagtaa taggataggc
    26581 gataggccac ataccctttg atggttagaa taaactgctc aaaatctgta tcgtatgttg
    26641 gctttagcat attaacaaag gctatctcca aattataaac cataagcgtt gaatcgttct
    26701 taacaatttc aattcttcct aacccaaagt cagaagatat aattaccgga cttgccttac
    26761 ccttcatgtg ggttgttgag attccatatt taaagggaat tgtcggtact atcggcggtc
    26821 tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta gtctcctaag tccctttgta
    26881 gcggatatgg caccataatc tcattttatc gaagtgagtc atggtttatc tgttggccaa
    26941 ccaaaattga tacaatctaa cgatgcaccc tctacggcat tctaggggag tgcacttgca
    27001 ttcacccaat aaaaaccggc agttagcaaa gaacttatgt agtcgtatgt tcttactgtt
    27061 aacatacaag aggtctcctt tccacaattg cttagcagta aacgggaatt tacaaagcta
    27121 atctcttcca ttttcttatc tttgttctaa aagtcccaaa tggagcaact gtatttacat
    27181 gaataaattt atatacttcc catacagccg ttttggttgc ctcgtctgcc cattttcttt
    27241 tgtgtgcagt gaatagatct tcatcactca ttgaatcaat catcatgtta atatcatcaa
    27301 tattacgatt gagaatttcc tctaattctt tcaatggtag atgtgcatag gtatttgtaa
    27361 accacttata taactctcct aactgattcc atttaaattg atcagaaggc gttttaacag
    27421 ataatccatt tctttcatca ttttcccatt ttagaattaa attcgtccaa ccaacttgat
    27481 atgctaaatt ttcagcaggt gttctatcca cttcctcaac tcttttatcc ttcaattctt
    27541 caggaatagt ttcaaattct aaaatatatt tagtataagt cctatttatt tcttttttta
    27601 attcttcttt atttttatat gttctcatta tattacctcc ataaattcct aattgtctcc
    27661 tttaattcat ctataatttc atccacgata ttttaatccg ttctacaatg catatctgat
    27721 atgcattgac tgtctaactc atatgtgaat gatttataaa atatcccaac gtctcatcta
    27781 aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa tatatttctt ttgtagtaaa
    27841 atcggactgg tttgcatatc attttcttcg taaatatcaa aatcatgtta actactatca
    27901 attactaata aaaactacca ctttaagcgt taagaactgt taagtttagt taagtgagga
    27961 tagttcacaa aatccattgt taaacttcac gaaagtggta aaactcaaaa tgctttatat
    28021 aggattacag catcgctctt tcttcgattg cgcgttgggt aaaaaatatt atgaagtaaa
    28081 aattgacgat gacacgttcc ttacggctca tcagatgaaa cagctacaaa aacgaaatga
    28141 tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca tattcacgcc tcgctcgacg
    28201 aactattaaa ggtcaagatt tacctgaaga tcatcctttt taggaaattg aagcttttta
    28261 tgttgcacaa tgcatttata atactacaat gatgttttca ccagatattc ttctagggtg
    28321 cggtgtaatg aaacagatac acttgcaaaa gaaaattcac cgcgaattta ctaaaattat
    28381 gaataattat gttaatcttc cgaaattttt ttccaaaatt aagggatcaa gctggtgtta
    28441 tagattgttt aaggttggca aaaaaataac ttctttttaa acaattatag aaaaccgata
    28501 ctctgttaag ttggttgaca cggtgtcgat ttttatgact ttcttattct gaaacttcac
    28561 taacggacag tacctaaaaa gtctatagta tggacgttta cgagtactat tttatcaaaa
    28621 atgctgctca tttcaactta tatgttttag tgaactaccc ccacttagc
//
LOCUS       DARBFO010000027        27719 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000027 DARBFO010000000
VERSION     DARBFO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.028"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(271..1215)
                     /locus_tag="SCK51_001312"
     CDS             complement(271..1215)
                     /locus_tag="SCK51_001312"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEG1251948.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1511..2455)
                     /locus_tag="SCK51_001313"
     CDS             complement(1511..2455)
                     /locus_tag="SCK51_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1251949.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2676..3890
                     /locus_tag="SCK51_001314"
     CDS             2676..3890
                     /locus_tag="SCK51_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1251950.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3912..5927
                     /locus_tag="SCK51_001315"
     CDS             3912..5927
                     /locus_tag="SCK51_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEG1251951.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6010..7383)
                     /locus_tag="SCK51_001316"
     CDS             complement(6010..7383)
                     /locus_tag="SCK51_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEG1251952.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7409..7693)
                     /locus_tag="SCK51_001317"
     CDS             complement(7409..7693)
                     /locus_tag="SCK51_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1251953.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7695..9710)
                     /locus_tag="SCK51_001318"
     CDS             complement(7695..9710)
                     /locus_tag="SCK51_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1251954.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9889..10191)
                     /locus_tag="SCK51_001319"
     CDS             complement(9889..10191)
                     /locus_tag="SCK51_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEG1251955.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEVALAGTNSGETEDKEESLAGTN
                     SGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10280..11311)
                     /locus_tag="SCK51_001320"
     CDS             complement(10280..11311)
                     /locus_tag="SCK51_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1251956.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11518..12393
                     /locus_tag="SCK51_001321"
     CDS             11518..12393
                     /locus_tag="SCK51_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1251957.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12545..13387)
                     /locus_tag="SCK51_001322"
     CDS             complement(12545..13387)
                     /locus_tag="SCK51_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1251958.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13479..15356)
                     /locus_tag="SCK51_001323"
     CDS             complement(13479..15356)
                     /locus_tag="SCK51_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1251959.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15353..16774)
                     /locus_tag="SCK51_001324"
     CDS             complement(15353..16774)
                     /locus_tag="SCK51_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1251960.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16777..20166)
                     /locus_tag="SCK51_001325"
     CDS             complement(16777..20166)
                     /locus_tag="SCK51_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEG1251961.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20423..21814
                     /locus_tag="SCK51_001326"
     CDS             20423..21814
                     /locus_tag="SCK51_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1251962.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21887..22534)
                     /locus_tag="SCK51_001327"
     CDS             complement(21887..22534)
                     /locus_tag="SCK51_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEG1251963.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22542..23228)
                     /locus_tag="SCK51_001328"
     CDS             complement(22542..23228)
                     /locus_tag="SCK51_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1251964.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23234..24307)
                     /locus_tag="SCK51_001329"
     CDS             complement(23234..24307)
                     /locus_tag="SCK51_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1251965.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24529..25203
                     /locus_tag="SCK51_001330"
     CDS             24529..25203
                     /locus_tag="SCK51_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1251966.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25208..26548
                     /locus_tag="SCK51_001331"
     CDS             25208..26548
                     /locus_tag="SCK51_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1251967.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26697..27683)
                     /locus_tag="SCK51_001332"
     CDS             complement(26697..27683)
                     /locus_tag="SCK51_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEG1251968.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8080 a         5243 c         5037 g         9359 t
ORIGIN      
        1 aatcaaatat gaacgaatta gatatgaatc attgtatttg ccgttgcgga aggatttttt
       61 aattaaccac agataaacat tgataacgat tcgcttattg taatcaacag attaaatttc
      121 atatgttcat atgtacggat acattttcgg accgattaat tttaaccatt ctgtttattt
      181 gcttacctat tagatatcaa taaaaaactg cttaacagct atttcagctg ttaagcagtt
      241 ttaggatttt tagttcatat tcattgtttc ttataataca gtgttcaata cttctcttaa
      301 agcacttgtt gatttgctga acattgcttt ttcttcttcg ttgattgata attcaacaac
      361 ttcacgaaca ccattttcgt caacgattga tggcacacct gtaaagatat ctttttcgtc
      421 gtattcacca gttaaataag cagatacagg caatacagct tgttcgttat tcaagatagc
      481 ttttacgata cgtgcagtac tcataccaat accatagtaa gttgcttttt tacgatcaat
      541 gatctcataa gcagcattac ggactttatc agcaatcaca tctaattcat cttgggcaat
      601 acgatggtct ttttctacaa tttcaaacac tggtttacca ccgacagttg tatgtgacca
      661 tgcagcaact tctgaatcac catgttcacc taagatatag ccgtggacgc tgcgtgggtc
      721 aacttttaat ttcaatgcga tttctttacg gaaacgagtt gtatccaatg ttgttccagt
      781 accgataaca cgtgatgttg gcagaccaga ttcgttccaa gcgatatatg tcaaaatatc
      841 tactgggttt gaagcaacaa cgataatacc gtcgaatcct gatcccatga tttcttttac
      901 gatttggcgc atgattgacg catttgtttt aactaaatct aaacgagatt gaccaggttt
      961 ttggttgata ccagctgtga taacaacgat atcagcatct ttacattctt catagccacc
     1021 tgaccataca gccacatttt catcgcccca agccatacca tctaaaaggt ccaatgcttc
     1081 cccttctgct ttttcttgat tcacatcgat caatactaat tcattagaga ttccttggtt
     1141 gatcattgca taagcgatac ttgttccaac aaaacctgta ccgacgatta ctactttacg
     1201 acttgttttt ttcatgaata aacagctcct tttcataact atttgttact ttcatgaaaa
     1261 gtatagtctt gtttattcat tttatcaaca tgttaattat aatagttgtt attataattt
     1321 ttgatggaaa cgaattcctg aatacgcata acagataaat gacaaatgtt tacacctata
     1381 tcccctgata atacaaccaa aagtttagaa ctgaaaataa atccaaaaaa attcgctgca
     1441 acgatataat aagacagcaa tcgcacctaa aacaattttc cttcatatgc tttagtgaaa
     1501 gattttcttc ttaatgttta tttctccatt cattgggagt acatgttgaa tacttcttga
     1561 ataatttgaa taaagaagtc gtttgtttat atccgatacg attagcgatt tcttccaacg
     1621 aaaaactcgt ttcttgaatc agttccaacg caaagtcgaa ttttttcttt agaaccagtt
     1681 cttgaaagga agcatcaaaa tacattttca atttattact aagatagacc ggattgtagc
     1741 caaaatgagc tgctgtttct tttaacgtac aagaagtaaa atgcttatcg atataatcta
     1801 atactctaag catctcattt tctgcgcatc catctgctga tgaaaagctt tgttgttcta
     1861 ataaatgagt caattcgatc agtaatagtg atgtatagag atttaacgaa cggatcttat
     1921 gactgtatgt atcaaaataa atcaacagca tattttcaat caaatgcgtc aaacgacttg
     1981 tttttccgcc gcgaaaatga atgaattgtg tatgttcccc tttttctttt gatgcattta
     2041 tcaaaaaaga agaaagtaga ttatttgtgc ttgccaaatg attcaatatc tctgtagata
     2101 aggtttcttc ttttaataag atgttgatta aaatatcctc ttgccctgtg tattcgattc
     2161 tttgagtagc ttctttatca atgatcacca cgtcactttc ttccatctct atcctttctc
     2221 cattaataat ttgtaaacat gtaccgctat acatataatt gatttcaaca aaatcatgtg
     2281 tatgctcggg aacttccgca tatcgacggt gtttactgat atgaatattt ccctgttgaa
     2341 atagatcccc tcggtggatt tttggcggtg tctgaagatg gtaagctaag tttaaatcat
     2401 tgatattcat tccttgttca cgctgcagag tttcgattga ttcttcgtta cgcaaaaaaa
     2461 atgtctaatt cagctagttc caattgactc cctccttttt gtaagcggtt tctttcgctt
     2521 attagttcac gctgtactgc tattatatgc aaagctattt ttggtttcat aaagactttc
     2581 tatggttggg acatgaactt cctgtgattc cgctattgag agaataacca cttcatacta
     2641 tatttttgat aagcgcttac aaaaatgaaa ggaacatgct tatgaataaa aagatgatga
     2701 aattttctct gctgtcagtc tctcttattt tgacttctgc tggtgcagtc acgtctgcaa
     2761 ttcccagtat gtcagctact tatgccacgc gttctttatc tgctgttgaa ttattaacga
     2821 ctgctccctc ttttatggtc actatttttg ttttgattag tagttttatt gctaggaaaa
     2881 tcggacaaaa acaaactgtg atcatcggat tagtgattgc atcgatttgt ggtatcctcc
     2941 ccctcttctc acaaagttac agtattgtct tagcttcaag agctggacta ggtattggct
     3001 ttggattgat caattctttg gcagtcagca tgattgctgc cttttatcac ggagatgaac
     3061 gagcctctat gattggtttt caaagtgcat ttcaaggatt tggtgcagct ttaatgacat
     3121 ttgttgcagg acagctagta cagttcggtt ggcaatatac ctttttagtc tacgccatca
     3181 gcttacctat tcttgtcttg tttgttttgt ttgttcctac acctgaaaca actgaacttc
     3241 aatcgcatgg cacagcccca acaagacaag taatcaaaac gaatcctaaa atgattagtt
     3301 atggtatttt tctcttgctg gtcatcgtgg tatataatgc tgtatcgatt aaattagcca
     3361 cagtcctgac gacttattct ctaggaaacg aaactaatgc agcgaatatc ttaacaatca
     3421 tgcaattggc aagtatggct gccggagttt gttttggctg gcttcagaca aaagcacatc
     3481 gctatatgct tacactttct ttactactga tgggaatcgg ctttgtattg attgccactg
     3541 tgataaacct gtatgtgatt gggtttggtg cgatcctaac aggaatttct ttttcgttat
     3601 tcattcctta cttattcaac caagtaagta tccaagcgcc caagtacgcg gagagtttca
     3661 atacttcaat cttattggtt ggcgctaatt taggtacttt cattgcacca tatggtttgc
     3721 tctttctctc ttttgcttct cttgggagta aaacacttgc tgttttcgtc aatggcagta
     3781 tcttgctgtt gattagcgcg cttattagcc ttctagtagt acacaggacg aatagaaaga
     3841 cagtgataag agtagatgaa gaaataaaag aacaattgac aacaaaataa atttgatgga
     3901 ggtcgtgtaa aatgacaaat gaaaaacata aagatagatg gctttggtat cctggagact
     3961 ttgagatccg ccatggactg ctacaaaatt tccaacggga agaacgaggt tttgactggc
     4021 cagcgtactg gtacatggat gattgccatc ggaacgtcaa gtttaagaga tattactttc
     4081 ttgatcaacc ttccatgttt aaagtgacga tccaaggagt tgggtatgta gaaattaatg
     4141 gacaaaaaca tccatgtgga aaatggctga cttgtccagc tggtaaagca aaaattcgta
     4201 tctttgtagg acatacatca gggctaccag cgatgtttat cgagggagac gaagtaaaat
     4261 cagatattgg ctggacagca agtaatttta ttgaagaata tccagctggc tggagtccgc
     4321 tttatgtcga tatcgcaaaa gatccaaatc aaatctatta tcaaaaagaa tgcgtccatc
     4381 cagtcactga aacggagatt ggcggaggaa tcttattcga ttttgggcga gcagtgaatg
     4441 gctgtgtaga actaatatct ctggatgacc atatcgaatc tatcactcta tgttatggag
     4501 agtccgatgt agaagctatg gatatcgatt attgctacta taagcaaata ggaatgacaa
     4561 gaaaaatgaa agctcgtaaa cgcgcttttc gctatttgtt tataccagag gtttctactg
     4621 gaatggttca acttcaagtt tttcatgaat ttttatctct ttcgaaacaa gctgcttttt
     4681 caagcgattc ttctttactg aatcagatct ggtcagtatc agaagaagcc ttcaagttat
     4741 gtagtgattt gttttttatc gacggtatca aacgagatcg ctggatttgg tctggagatg
     4801 cgtatcaatc attcatgatc aatcaatatc ttttttttga cgaggaaatc aataaacgaa
     4861 cgatattagc attaagagga caaaatgaaa tcaagcagca tatgaatact atcgttgact
     4921 attcgatctt gtggttgatt ggtatcgaaa accactatat gatgacgaaa gactatgaat
     4981 ttttacgcat cgtctaccct aagatgcaat cattaatgag atacttgatg gagcaaacga
     5041 atgaattagg gtttatttat gggagagcaa aagattggat atttattgat tggtctgaaa
     5101 tggataaaga aggaacgctt gctgcagaac aagtcttatt actgaaagcc tatcattcca
     5161 tgatcacttg cggagatatc ttagctgaaa catatggaga gaacgaaaat tatcatactc
     5221 atctctatca agaaaaatat caactattga aacaaaacct atttgactac ttttgggatg
     5281 aagaacttgg cgcttttatc gattgctatg aatctgggag aagaaatgtg acgcgtcatg
     5341 cgaatatttt cgctgtttta ttcgattttg ctaacaaaga ccaacagcaa tcgatcttaa
     5401 agaatgtctt attgaatgac actatcacac agattactac tccatatttc aaattctatg
     5461 aacaagatgc tttatgcaaa ctcggacaga cgaatatcgt ctaccaaacg attcttgatt
     5521 attggggagg gatgttatct catgatgcgg taactttctg ggaagaatac gaccctactc
     5581 aaacaggaaa tgaaaagtac agtatgtatg gagatcctta tggaaaaagc ttatgccatg
     5641 catggggagc aagcccaatc tatcttttag gacgctattt tatcggtttg tatccaacaa
     5701 agccaggtta tgaagaattt atgatacaac ctaatctaga ttcatttgaa acactgaatt
     5761 gtacgctacc tatcaaagat ggaaagattt cgcttgaaaa aacaaaagat aagatcgtga
     5821 ttacttctga tagaacaggc ggaacagtga aaatgaatgg taaaagttat cctcttctag
     5881 caaaacagcc acttgtctta acaagctatt caaaaataaa tgcataaaac aaaaaactgc
     5941 ttgcgacata tcagaagact ttaaatcttc ttctgatatt gtccaagcaa tttttatttt
     6001 gaagtttgct taattctgtt ctttagaaac aatgtttgtt ctttttccgg caaaaagaaa
     6061 gctagataat acgacaagta aaaagaaagc gatgattcca actgtcaagg ctatttgacc
     6121 gtattctcct agtttcccaa gtacaagtcc tggtactaga taatcagtat cggcaaatgt
     6181 agaagaagcg attttaagat ctcctaatac tggaaggata aataatggca gccaactaat
     6241 caataagcta ttgaaagcag aacctaatac cgcccctctt ctaccgccag tcgagttgcc
     6301 gaagacacca gaagcagctc cgcagaagaa atgtcctact accccaggaa tgataactgt
     6361 agtttgcaag cctatcatag caaacatgga tagtgttcct acaaagaagc taacaaaaaa
     6421 gccaattaat acagcattag gtgcatatgg aaaaacgata ggaacatcta atgctggttt
     6481 agaatcgggt acgagttttt tagcaatccc ttggaatgct ggaacgattt ctgccaacac
     6541 cattcgaacc ccttgaagga cgataacaaa cccagctgtg aatgttccag cttggatcag
     6601 tgcaaagatt acagcgttgt ctccattgct tagttctctt tcaacaaatc caggaccagc
     6661 taacaaagca agtgcgacat atacgatcga catcaagagc atgatcgata cagtagaatc
     6721 tcgcaaaaag ctgaaacttt taggaatctt tatgtcctct gtagaacggt ctttgtttcc
     6781 aaaaaacttt ccgatccatc cactagaagc ataaccgata tttccggtat gccctaacgc
     6841 aacttgattg tttccagtga tgttcttcat aaacggttga caaagagcag gcgtaatagt
     6901 taatagtgtt ccttcgaaga ccccgcctaa taagatcatc cacaaatttg cagtaaaccc
     6961 cgcgcttact aaaatgactg ctgttagaca agaaacataa agcattgctt gacctgtaag
     7021 aaaaatgaat ttatatcttg tgaatctagc aagtaaaata ttcacgatca tccctactag
     7081 catgatcaaa gcagtaggtg taccgtattg ttgcaaagca atcgccacta ctgcttcatt
     7141 gctaggaact actccttgag tatgcagagc tgtttggaac atcgaagcaa aaggggttag
     7201 tgaattactg attaaaccag caccgccgat cagtactagg aatccgacaa atgttttgat
     7261 agttccttgg atcacttctg aaaatttctt cttctgaaga acgagaccaa gcagtgcaat
     7321 caaagaaacg aggatggagg gctgactgac gattgacaca aacaaattca acaatttatc
     7381 cattgatagt tcctcccttg aatcattttt attcttttga caagatcttc gttacttttt
     7441 cttttacttc ttcgctgtca atgatcgatt gcaaaggtac gagcttttca tcaggtatag
     7501 aagttagcgt atctttcaaa gaatgctcaa tgaagaaata atcagccata tcgctagata
     7561 cacttccaac atccgtatga cttacttcaa actcgtccac atctgctcca agatctgcaa
     7621 gtgcttgttt gatgttcatc tctaccataa aacttgttcc taatccactt tgacatactg
     7681 caaaaatttt catatcattc gtctcctttt cttatgatcg ctaatatttc ttcttttgtt
     7741 gatgcgtgta ataaatcttg aagattttgt ttattagaaa gtagctttgt taaatttgcc
     7801 aaagcccgaa gatgtgcctc attatcgata gcagctaaac agatgaacaa actaatggga
     7861 tgggctggat tatcaagcag atccacaggt tgttgaagtt tcagcaaaga catccctact
     7921 tcgtttacgc catcttctgg acgagcatga ggtaatgcga tgtgatcgcc aatatggata
     7981 aacgcaccat actgctccac gcgctcgatc atagctttga tataactttc ttctatttta
     8041 ttttctgata atagcggctg tgcggcaaat gtgattgctt cttgccagtt ttttggttga
     8101 tccgataatt ggatcatttc ttctgttaat aattcagata gcataggacg ttgatcctcc
     8161 ttcttttcta aaagtttgtt cattttacga tttaatactt gatagagctt ttcttcgtct
     8221 acgccttctt ttaattcgat ataaggcagt agcgccgaca acaattcttg ggcagaaggc
     8281 attgaaaatc ctggtatcag ccaatctctt tgtacttgat tgatcagctg atttttttcc
     8341 aagtcagtca tgattggatg cagaacatag agagactttt tcttatcagt aagtgtgaga
     8401 ggtacagtag aaaaaataat atcgtaagaa gtttcgtcaa cctcgtgtaa gcggtcagct
     8461 gaattagtct ctgtaaaaaa gatcatcggg aataattgct gaagttcact ttttaagatc
     8521 aatgaagagc taatgccact tggacagacg atcacggctc ttatttttct atttctttct
     8581 tctgcgtccg cttttctgat ctctccgcca aatagtatag tgaaaaaacc aatctcttct
     8641 tcagggatgc tcttccctat tcgtctttct aacggaagta atgcttttct tgtcatttcg
     8701 tagatggaag catattgatg cttaatttga tcgatcatca cattaggtaa ataaaaagaa
     8761 taggaaatac gaaaataggc agggaccaag tgataaaaga cattcatcaa taattcacga
     8821 aacgaagcaa attctacggc catcaggaat tccatgcgat ggatgacttc tgaagaacat
     8881 tccagcaaaa aatcaagtgc aggctcctgg acatctcctt caacgatcga tgctaagcaa
     8941 agagagaaaa atataatctc cgaattttga tgttcggaat agccaaggcc agtcaatact
     9001 tgtcgactaa tgtcagtgac tgcaagagaa gccaacagtt cagcatcttt taccagttcc
     9061 atggatgttt ttccaattcg aatggttgtc aaactcaaat aataagctaa ttcatccatt
     9121 cgatttggtg cgaatcgaag atctgatagt gaaatcgttt tatctagcaa atctctaact
     9181 ttggcgtaat aatcaatgga aagggttgcg gttaaatacg tgagcccaaa tcttccagca
     9241 agtgactgca tcaaacgagc agtgcgattt tttgcttctc ttcttaatat tgcttctgac
     9301 cctagcagcc taaaaccagt tttcctagta tacgacagct gacaatcttc cacttctaat
     9361 tcatttctta tctttttgat atctgacagg actgtccctt tagaaacctg aagaaaatct
     9421 tgaaaatgaa aaacagaaac agacgttgca gtcgaataaa ccatcaaata aagaagatct
     9481 cttcgctctt gttcggtata cacagcttca aatcgagtaa tatccaaaaa tttttctgcc
     9541 cagtcgattg ctcggaattt ctttatacgg atacgggtat catccacata ttcggcaaaa
     9601 ggagttaatt gtttgatcgc ctcgcttgta tttacttcga tgatctctgc tgctttttct
     9661 agactgattt ctcttggtgt tttcaggaga taactaattt tgtagttcac agcgctgttc
     9721 acctcctact tatattctag tatggtagcg cttttaaatc gaggggtgtt tggtacaact
     9781 tacagttgga ttttttttac gactgtaccg aacgatttct atcgaatgga tacagaaaaa
     9841 aagcaggtga ccaaatgtct cctgctttta tgaattgatt tcgtccgatc aatgatgatc
     9901 atgtttattt tttgaggtca gttcttcaaa tttttctttg acatcttcaa ccgctttttc
     9961 gatatctttt tcaaagtgat tgggctcatt aggctttgtt tcctcttcta cagaatttga
    10021 accagataaa gctgtttctt tattttctgt ttcaccagag tttgtgccag ctaatgattc
    10081 ttctttgtct tctgtttcac cagaattagt tcctgctaaa gccacttctt ctgttttagg
    10141 ggccattgaa cctcttaatc gtttgtgttt ttcaccgtca taatttgtca tgttggttcc
    10201 tcctagtagt tctatacttt ttcatcgtac aataagacag tttatgcgtc aaaaaacaaa
    10261 cccgattctt acaaatatgt catatttttc cttctgattc ttgaatgctt ttgattcctt
    10321 cttccaaaat tttcatttgt aagattgttt cttcatcttt gatgactttt tctgcgccat
    10381 taactaatgt ctcgtaaatc ccttcgtaga cacggctgta atcaccaacc tcggaaatta
    10441 ctttttcttc atgataactc ccttgttcat ctatataggt caatacacca taatgttcgg
    10501 gcaaatcgat accaaagtct tcatgatcag gcatgtaaaa cattttcaga tgttcttctt
    10561 gtcgatcttt tgtttgtttc acgaacatcc ctttttttcc gtaaacgacg aaacttggac
    10621 gttctttgat gcggaaataa ctggatttta tcgaaatttt catatttcca tagaaaaaat
    10681 ccagatcgaa gtaatcattc attcgtcctt tccctaaaag ttgacgtaca tcatagctga
    10741 tatcatctgg ttcgccgaaa taagaaagaa cttgatctac tgtatgacaa gcatgtccat
    10801 ataaatagct gttcgcagta gaaaattcat gcgtattttc tggtacttcc ggtctgaaat
    10861 aatcataatg catttctact tctaaaatat cccctaattt tccgctttcg attacttttt
    10921 ggactgtcaa gaagtcagaa tcgaaccgtc tgttttggta acattggata aatagacctt
    10981 tttcttttgc taatgcaaat aactcttctg cttcctttga cgtttctgca aaaggtttct
    11041 ctactagtac attcttacca tgttcgagtg ccatttttcc atattcataa tgaaattgac
    11101 taggtgtagt gatgacaact aattggatct caggatcatc ccaaagatca gacagctgat
    11161 cagtatattc gatgtcttcg aatgttttcc acactggttc agatggggaa aaaatttttt
    11221 tgatattgat tttgttcgta agttttcttg aaaaaggcaa atgatagcga tttgtactct
    11281 ttccattgcc aagataagcg attgtaagca tgagtggtgc ctccttttat tgatgttttc
    11341 agtatatcct agaaaagcgc agaaagtatt tataatagct attctggtaa aaaagttcag
    11401 aaaaacactt gttatcattc ggtttggtga aattaatgtt caaagtaagg ataaaaatca
    11461 aaaagatagt agcggaacca agtgtttatc agtaagatag aatttggagg tcagatcatg
    11521 cttattgaag aaaaactaac aaaacaagag ttgtttacca cgacagagaa acggattgtc
    11581 gattacatcc gtaggaatat cgaagctgca gtctacatga cgattgaaga attagcaaaa
    11641 gctacgtata cttcccattc agctatcatt cgtttatgca aaaaaatggg attcagcgga
    11701 tttaaagaat tccgtttcga gctggggcgg gaagttcatc aattgattgc tcagttcaat
    11761 caaacgattg atcctaattt tcctttttca agtgaagata gcactcagca aattgcaaag
    11821 aaaatggcgg atttatcgat ccagtcaata aaaaaagcac aattgcagat tgaacatgaa
    11881 gatttagatc aaatcggtaa agtattgaac aaggcacagc gaatctttct ttttgcaaaa
    11941 ggtgattctc aaatcacggc aaggaaattc cagaataaaa tggtcaagtt gaataagttt
    12001 ttgatcatgg ctgaagaata cagtgattct tcttggaatg cagccaattt gactagcgag
    12061 gattgcgcca tttttattag ctacagcggt cgcatccatc attatgaacg aataatgacc
    12121 tatttgaagc atgtagaggc ccctaccttg ctgattaccg gaaatcagca ttcagaaatg
    12181 gccaaacagg ctgatatgtg tcttgttata tcgcaggagg aatatgattt tgctaaagta
    12241 gcgactttct cctctcaaat tgccttcgat tacgtattaa atacgttatt ctcagtgata
    12301 tattctcaaa attttgaaga gaatatccgc aacttgaaaa acaaacaaga attgatgcaa
    12361 aaaggccttc ttaaagaata tcggaaaact taagaaaaga tcctcattta gtaataagaa
    12421 agatagtgtt tgataggctc tctttgatat catcataaaa acaggctgaa gttttctttt
    12481 tcaaacctca gcctgtttgt ttacggaacg attcgaatag atatattata gaaatgagga
    12541 atagctaact tctgtttctt attttgaacg attgacgaaa aagatatata agaaggagga
    12601 aaatcagtat acatgcagct aacaatccat acaggaaaaa tcgtttgttc tcggatactt
    12661 tctgtacttc tttttgaact tcttttgcat gataaggaac ccggtgccct ctgacaagta
    12721 agcgatgact gttgaccata taaggtgtac aagttaggag tgtcacgaaa tcctgccctt
    12781 tttcgattct cagatctttt gtatcagtcg gttctaccgt cttgatctga tcgacttgat
    12841 aagcgagtgt ccgtccattg atttcgatat agaaagaatc acctaaagtc agtttcggta
    12901 agtctgtgaa caactttgct tgaggcagtc ctcgatggct ggaaataacg gcatgtgtat
    12961 tttctccccc cgtaggataa gacgttcctt ccaataatga ggcacctttt tctagcagca
    13021 cagtattcgt ttcatcaaaa attggcaaat ggacattgat ctcagggatc gtcaaaatac
    13081 cgatcgtatg tttctcaaaa taggttctat cctttttcgc aggttctttt ttcttagtaa
    13141 agggatctgc acctggattt ccaccttctt ttgctaattg ctggtttttc tttgtcatgt
    13201 tttcttggat tttagccatg acttcttcat tttctttgac cgcttgttgc tgataatgag
    13261 agatgatctg ctgatccaga taattattca gcgcgtcgct aacaaatgga taagctagta
    13321 tccctattcc tgagagaagc aagaaaagca tcagcagatc tagaagtttc cttttctttg
    13381 gtttcatttg tgttctcctt gtgatgactg tttgataata aaagaaaaag gagcatttgc
    13441 gtaatccgct gctccttttt ccaattcttg caaaaagact aaatctgact gtgcttgcgt
    13501 ctagcaaagt acagtccagc aatcagtaga aggactactc ctgcaccgat atacacatag
    13561 attcccttac cgcctgttga aggaagtgta cctttgtgtt tatttggtat tttttcagga
    13621 gaaatcaact gtcctgctgt tacataagat tgttcatcga tagtaaatgc tacacggttt
    13681 gttaatggaa catatttttc tggcgctttc gtttcttcca gatagtacgt gccatatttt
    13741 agacctgtca catcgattaa accgttactt gtggttgtaa aaaccgttgc tgattctttc
    13801 gccgataccc agctgacggc ttttgtggat gggtcgatcg ataaatattt cgctgtgtca
    13861 ctatcttgat cacgaacgac gaattctgct ccagcaagtg tttggtctga tgtaacgtca
    13921 ccatctactt taacaaatcg tttgccccca gtaacgacat cgactgacgg tggtgtttga
    13981 tcatttgtat gcccgttatc gacatttgct tggttagaaa atcctttggt tggatctgct
    14041 ttttcgttca aatgcatata gtaaacgaat ttcaatgtac cgccaggagt taatgaagga
    14101 atataattcg gatcaactga aaccgtgaat ccgtctgttt gctcagtgac agaataattt
    14161 actgggtcga tttctttatt tccatcatat aaggcataag catacgttcc tgaagaatca
    14221 ttatcaaatg ttaaagcagc gtcatgagta tcgataagtt tgaatgttgt gtacttgttt
    14281 tgcgttcctt ctttatcagc gatacctaat gggatattga cagaaatctc atattgaatt
    14341 ttttcaccga ttgcgacatc ttttccattc aattgaggtg ttgttttatc aactttagac
    14401 gtatcatttt tgatggtctt ttctactggt gtttggctat ttgccaggat ctcaaaaggc
    14461 gtgattgttt gcttttcaat catttccgca ttatcaggag cttttacttc ttctaaatta
    14521 tattttccaa cttctaaatg attaacgatc aactggcctt tttcaataga tgcctcggca
    14581 aagtcattgt tgccgatgtc ataagaatga ccagtaatga aatgtttggc tttggtttga
    14641 tcagttgtcc aagtgtacaa tccatctgtg acactttgga tgtatttttt gacgcttggt
    14701 gttccttctt ctttagaaat aataaattct gctccattta gtgcttcgtt ttcggcagta
    14761 ccgatttttg taactttcaa cgttccatca ttaccgactg tatttttagg gtagagatgg
    14821 atagtatcta gttcttctgt cccgtattta taagagccat ctgcttgttt gatcatctca
    14881 tatacaggga aagctaaaac catgtttgcg gcagctgaca ctccgtcttt tggttcttct
    14941 ttgatcgtgt agactgcatc tttcccattt tgttttttag gtaaagataa agtgacattt
    15001 ccatcagcat ctgtcgttcc tgaagcaact ggtgtaccag gagtcaaaga ctggactgct
    15061 tgttttgctg catccacgga cgctccttta tctcgttgcg cataaaattc ttgagtgacg
    15121 ttataaactg aaaatgaaat atcggctaat ccttggtatt gatcgaattc gctcatttct
    15181 ttcccgctgt tttggattaa aggatcgggt aaatcagtca ttttcttttt atgaaggatg
    15241 acttgagctg ctgtctcctc tgcaaacact ttttttgcgc cgaagctgtt tgtgagtaat
    15301 ggtaaaataa ggaaaaggac tcctaacata acgtttattt ttttatgatt tttcattttt
    15361 tcactcctgt tctttttttg ataaacaacc agattgcaaa gcttgcgaat accataccaa
    15421 caacgcctaa aagtgtattt ttcgtttcgt ttgtttttgg aagaaatgga cggctatttt
    15481 cttttttatt gactactttc aatatactgt ccgcctcttt atcctcagaa agaaaagttg
    15541 aaatggtaaa cggaatttct gtttcactta aaatataacc agaaggtgct tcgatctctt
    15601 ttaatcgata acttcctgtt ttcaacccgg aaattgaaaa agcgccattt ttatcggaaa
    15661 tcaggtgaag cgcatctgat tcgttctttg tccacttgta tccgttggct gtttgctgga
    15721 ggtaatttcc tgctttgttt ttaataagga atactgcgcc agctaatcgt ttatctggtt
    15781 tatttgtctc gattttgaca aattgcttac tccctgtata tacagagact ttctttgtac
    15841 tgacaccaaa accaaaatcc agtgttccgt tgttgttgat ttccttgttc gcctgtgctg
    15901 tactgcttag ctgcatctga taagaaatgg tcagtttttt tccagcatat ttttccaacg
    15961 aagggatcga aaataaaaca tcaaatccgt gaggtttctt gtgtatgaca aagccttctg
    16021 tcagcttttc tccattcgat gaaatcgtta aactattagg taaaaacgtt aatgcttcat
    16081 ccgcactatc tgacaattcg aacttttgat agtcaaggat atttacagga attgtcgtat
    16141 gcaaggaata agtgattttt tcaccaatcg taaaatcatt tctaggctct tctaatacct
    16201 tctcaaaagg tggatcaggg tagtcgtttt cttcattttt aggatacaaa tggatagttg
    16261 aaagtttttg attgttttgt ccatgtacag gaagaacaac taccatattc tctgcctttt
    16321 ctttgacgac ttctggtact ttggattcaa tgaaaagata cgctttatct cttttttctt
    16381 gatctttagc ggccagagaa aaagaagcga taccatcttc gttgttaaga gttgtagttg
    16441 ttgcttctgc ggtaaacata ccggaagacg caccgttttt tgcgatctct gcttgtgctt
    16501 cttctaccgt tttgcccttt tcccgtagat ggtaaaaaga atctgtgaca tcataaactt
    16561 ggaatgtgac accatttaat cctcgatacg tttgtaataa agctttttct tgtccgtcat
    16621 ttggatgatt tttcggcagt tgcccgtttg ggaaaagcaa tttgtgtaaa gtaatctgaa
    16681 ccatttctgt ttctgtttct gttgctacct gagtaaaagc tggtttaaat agtaacaaga
    16741 agagacacat actaagccaa ccaagttttt tcatatttag ctgccccctc tttctttttt
    16801 caaaaaaaga taccaaatag aaaacgcaca accaatcatc cctactagat agattcctaa
    16861 acggccaatt cctcccgttt caggtaatgg tacttttgcc tgattcgtga tgtctaaaga
    16921 aatctggttg tttttggctc ctggtgacag aacagattca tgatcttgtc catccacttg
    16981 aattgaccca tcttcgtgta taactatagt aactggctct tttagacctt ggtatccttc
    17041 tggtgtaaaa gtttcggtca gcgtataagt tccaggtttt aaattttcga atagaaaggt
    17101 atctgtttct tgtccatctt ttggcgattc taagtccatt tctggtccct gcagtctgaa
    17161 ttttgctcct tttaatgtct gtccgttgtc ttcttttttg tgtaccgtta gatcaaacgc
    17221 ttttagtcga ttttgatgag tgagcgtcca tacattattt gtcatctcgg tattttttcc
    17281 cgtgtaaatg atttttccat atttatcgat ttggaattca taatttcccg gcgaagtatc
    17341 atatccggca ggaccagctg tttcaacgac tcgatattta ccaggtgtga gtgaatcaaa
    17401 actggcaaga cctttttcat tcgttgtttg gctgtcaatt ggctgataag taccatttgc
    17461 ttcttttttc tgtagggaaa atgctgctcc tttaagattt atctctttgt ccgtcccttg
    17521 catcgcatac ttacgtactg ctacaggaac ttcaacaaaa ggattttcaa tagtcaactg
    17581 gatcgtatca tcggaagtag tgactgtttt tccatcaatg gttaccgtac catcagaagc
    17641 gatcttgatt tcccaagtag tctttttgct taatccatgc ccttctggag cttttgtttc
    17701 tgtcagcgta taggtcattt ctttttgcaa tttgacattt tctggaagag aataggttcc
    17761 gtcgccatga tctgttagta aagtatcaat agaatctcct gttaatttga aaacagcgcc
    17821 aataagatcc ttttcacctg tagaggaatt tttcgttatt ttcaagttta acggtgtgaa
    17881 ttgtttagta tttttccatg tcattgcatc tatttgttga gaatcgtatc ctggtacagg
    17941 taattctttg attgtttggt aactgaatgc ttgaccttga ttattgtatt gaggtagtga
    18001 taatatctct tgataacttt ctccgctatt tgcagataat ttgtcaatgt ctgcacgttc
    18061 ccacgtattt gttgtatctt tagctggttt aatgattcga atatatccgt ttttccaagc
    18121 tgcagcattt gtcgtatgtt cccgttgaat ctcaaaagta acttgatctg gacgatcagc
    18181 tagattgttg tcaaattctt cccataactt tttgatgtga agactgactc cgggagcttt
    18241 agcagatggt ataccgaatt cagctaattc attggtatca atgtttggct ggaaagttgt
    18301 cctgccgttc atttgatacc agaaatttgg atggaagtcc tcattttctg tttggattct
    18361 cacttggtaa tggatttgga tttcttggtc ttttcctaaa tagatctgat tgctcttgat
    18421 ggtatttcct tctatggaaa tagatggagt gaccgttgta ggacttgtcc ccacactctt
    18481 gactgaaagt gtaccaggct gataaatgaa tggttctgcg attggatcat ttatttgtcc
    18541 attgcttaca gttgctgaga tctgtacagc ttttttggct aggtattcag agatatctgt
    18601 tgcatgatca gctgattcat agtaaagatc gcctttttca tctgatgaaa ccatttgtcg
    18661 catacgagac tctacttctg cttttgagag accagctgcc ggatcgcttt gaagttggat
    18721 gccaagacca tgtatttcga ttcctcgttc tttgagtgcc atcgcttctc cgatggttgc
    18781 gataaacgta ctgtcgattc ttctggatag attgttttgg tcaggtgcat catagattct
    18841 tgagattaga gaagtatttc ccggccgatc ttgcgtatta gaaaactgag ttccgtaata
    18901 attgctgctt gattgtgcgt gtacccgctg tactttatag gaaaatgttg gtacaccatc
    18961 cgtcagcaaa acgatcactt ttttatgtcc atttggaacg gatagcatgc ttcctgcatc
    19021 tcttagtgct ttttgtgtaa aagtaccacc atttgtccgt gaaggtgtaa tggattttac
    19081 ttgatttttc actgaatcaa atgaacccat ctgtactgca ccgttactgt agctatatcc
    19141 ttcgcttgag tagccgacat atcccatatt gattttgtct gtgataccgc tatctgctaa
    19201 agtatcgaca aaacgatcga caccaatctt tacttcaccg atccgattat tgtcgttcat
    19261 acttcctgac cagtctacga ccaatacgag atcaagaggc gtgatatctt tttgtacatt
    19321 tccacgagca ttcaaataaa catcaaacaa tccgggtgta cttgtttcct ttgcatactt
    19381 tcgtatcgca tagtctgctt gattaccggt tcctccatat tcgatatacg aatgggtccg
    19441 atcattagga tctccattcc aagaatttat accatcccat tgatttgttc cgtttttatt
    19501 gccttgatga tcaagaacat ttgtatttcc tgtaggttgc caactggctt taggataagt
    19561 gccctgatca tctgttgtat attctgtagg aatcgtttga ttagatggca cactcacagc
    19621 tggcaaggat aatgtaggca atgtaaatgg gctgacagaa ttttcgtttt cttttgatga
    19681 ttcttcttct atttcgtctg gctttggtac tgacacatct gtatctttct tattttctgt
    19741 ctcatcaata atgtcatatt ctttattatc gacgatcagc ttgtttgcct ggctgtggta
    19801 aagtgctgga tcgattggaa aactcagatc gatagtttgt ttagtgtttg atgagatttc
    19861 caacgtaatt ttcccgctat ttacctgata agcatagcca tctccttctt ttctttccac
    19921 atcacttaag taattttcga ctagaccaat ttctttttct atcttttcag cactggtgtt
    19981 attcgcattt agcttgatgt tcagtgcttt ttttccagac tgatccgaaa tgacttcagc
    20041 tgaaattgtt tctaaaactg ttggatcatc tgtagtgtct gctattgcag ataatggcac
    20101 aaagtttgat aaactcaata ttatcagcat gaaaataact ttcagtttct tccctgtggt
    20161 cgtcattttt cccctccttt ttcttttttt ctgttttgac gtgaatagtg aaaattatta
    20221 aaataaacag gcgttacata attttattta ttttaatatg ataattatta tacgaagggc
    20281 gctaaaggga atcacaccaa ttttcatacc aattggtttc atttatgaag ttgattcctt
    20341 cgattaatga gcgtttcatt gaaagaaaag ggttgaggga gaaaaacata tgatcaggtt
    20401 gttggaagga aactatagaa agatggaaaa aatcattcga attctgtcca tttctgacaa
    20461 gtggatgact aaaaaagaac ttgccaaacg aattggtagt tcagaatcta catttgtaag
    20521 gtatatcgaa gagatcaaaa tccgctgggg aacggatgta acgattcaat cttcacaaaa
    20581 attggggtat cgtcttgaac atttgaatgt accagcttat ttaaatgttc tgatggaaat
    20641 gtcgcaaagt tctacaaaca gtcgtttatt aaatgagatt attgtcaatc cgggtaaaac
    20701 gattgagtat tattgtgaaa aaatttccat cagccggtct tcttttacac gtaagttgaa
    20761 acaatgcaat caaattttag aaagctattt attaaaagtg atcgttgacc aaggttttca
    20821 gttaactagc cttaaaagtg agctccagtt gcgtatcttt gttacctttt tctttttaag
    20881 ttactacgga agacgcacgt tgccatttcc aatcgaaaaa aagaagatca aagaaattat
    20941 ggtcaagaat aattgtcaga tgaatttgtt tcaggaagaa agcccatatg agcaatcttt
    21001 ctttattatg tattttgttg tgcatctcat gagagaatcc caaggaactc catttgaatg
    21061 gaaagctgga gactcttatg tacagttgat aaatatcgat aaaaaagact tcttaatctt
    21121 gaaaaatctc ttaggaaaat tgaagtacag cagctatata aaagtagtga actattttgt
    21181 caatacgatt ttttcagaat tttcggagaa agaaaaaaat gaaatcagtg agcagatacg
    21241 aaaaaatatg tcctactgtt cgttttcatc cgctgattgc aaagaaaaaa acgaagaata
    21301 tgttgttcaa ttattaacgc aaatttattt tttctcccgt attttcccat acgatacgac
    21361 tcatttgacg atccgtgcaa cttactttgc acagcagatg caacaccgcc agatacgtct
    21421 atatgctgga tttcgtgcag aacttcaatt ttattccact ctactgaatc agaatctgat
    21481 ttattattta tctagctata tcttttggat gcttatcggt aatcctgaaa ttcaccatta
    21541 tacttctgat aaaaaaatac tgattatcag cgacttaagt cttcgacata gtcaatatat
    21601 tgaagaatat atcagtgata ttcttgctgt gcataaaatt cactctgaga caactgctat
    21661 tactgaagat cagctgtcga aatacaatct tctcgagtat gatctgatcg ttacgaatca
    21721 acccattttg aatgcccatg ttcctcatat attgattgat gatagtgtgt cattcgcaaa
    21781 tgaagaagaa ctcatccgct tattcgagtt atgaataatc aaaaaacagc tatgtataaa
    21841 ccaccctgat aaaaatttca ggacaaatac acatagctgt ttctttttat tgtattcctt
    21901 cagcaatcca acgagattta tagcgaaagt cttttaacaa ctcttctttt gtccaattat
    21961 ttgcaatgag atccgtcaaa gtgttcatta cgagggtcat ggaaaagtat aggaaactaa
    22021 ctgcttcttc cgacgattga atggagaaat gattgattct taataattcc agccattttt
    22081 cgttcattgg agaatcagcc gatagttctc tacgtttggt tgtctgctga tcatttcgct
    22141 gggttaacaa gtataacctt tgccaataga tactttcgtg atctaagtcg cagcaccact
    22201 ctaaatattt ttcgatcccc aaaaagaaat gttgtttgtt ttcgctaatg aattttaaga
    22261 tatcttgatg gactatgtga gaactgcttt tgatcgtata caaataagca tcttccaaat
    22321 cttgaaaata tttataaaaa gcaccgcgcg acatccccat cttgtcaacg atttctgaga
    22381 ctttgacttg gctgatgtgc tgtgagcaaa atatctccag taataggtca tcgattctct
    22441 gtttctttgc gttcgataaa ttgaaaaaag tttcttttgg catccacatc tctcctttat
    22501 ctctgattat acgtggatag cccttcattg tcatgaagga ttcattcctt ctttttctct
    22561 tcttctgtca agtacccatc ttccattcga taaacgcgat cactccattg aatcatacgg
    22621 acatcatgag taaccataat agtcgctttt tgtttctcat gagcttcttt aactagtaat
    22681 ttgacgactt catatgcatg atctgtatcc aaactagctg ttggttcgtc tgctaagatc
    22741 aagcttggct cattatataa agcgcgagca attgccactc tctgtctttc tccaccagaa
    22801 aggtctttag gataactatt tttaagtgac tggatatcta aagattgcag caaggaatcg
    22861 atttttgttg aatcttcttt ttttgaagta acttggtcaa ctagttcaaa ttgttccttt
    22921 accgttaaaa aaggaatcaa attagagctt tggagaataa atcctatttc tttaaagcgt
    22981 aaatctgctc gttttttttc tggaagctca gttagtgagt gaccgttgat cactatttct
    23041 ccggaagtcg gtgtttgcaa tcctcctgaa atggtaagga aagtactttt acctgaacca
    23101 gaaggaccaa tgatgctgac gaattctcct tcctttacat aaaaatcaat tgctttcaaa
    23161 gctgagacct ttgtatgacc agaaccaaat gttttggtaa cttttttcat cgttaatatt
    23221 tttttcattt ttattaacct cctattgctg taattgggtc tactttggtt atcgtacgta
    23281 tcgaaaacaa tccgcctagc attgcgacaa tgatcaaaat cccactatat agcaaccatt
    23341 gtgaccaaaa aacagcaaaa ggcatcgcgc ttggtaaaat cagactagtc aaataagcaa
    23401 acaaaagagc aagcagaacg cctaccaccc caacaataaa ggactgtgct actaaagatt
    23461 tagcgatgaa gaaatttttg atcccttgtg ctttcattac tccaaaaata gcagtcttct
    23521 gaagtgtcat cacgtacatg aaaataccga ccacggcagc aaccactaag aacaaaaagt
    23581 atatcatcgc atttaaggtc atattttggg cagaatagcc aggaatactt tcaatgaatg
    23641 tcccaatagt taataactgt agacccttcg atgctttttc tttcgaaatt ttagctgaat
    23701 cctttattac gatcccattg atcggctgtt cttcttcaga agcaaacggc tgttgtccat
    23761 acttcaaagc agtccacgta tctagggacg catagatgac gggagaaact gtgtaataag
    23821 tttcaggaaa gataccgaca atcgtcaaag gatcgtcgta actgccaatt ttgatcgtat
    23881 ctccaatttt atagcctgaa tcagccagat tttgagagat gatgatttca ttctttgttt
    23941 taaattcatg ccctttcgtg agttttggta ataaaaatgc ttctttcgtt gtgccgaaaa
    24001 ctgtaacatt ttctttgttt tttcctttga ttgctccgct gtacaatccg attggtgctt
    24061 tctctcctcc ttcaatgtat ttcagatctc ctcttgttaa ttgtgaagct gcaaatactt
    24121 gatttgcatc ctccgacaaa acaatctctt gtgcgtccca atcatcgatt gcttttttaa
    24181 actcttctga aagtccattc gctagtcctg acaacatgaa tacgacatac gcaatcaaga
    24241 acataatacc gacgatcaag ccatatcgta atttagaata gcgaatttct ttgatagcta
    24301 gaaacatttg tttttcctcc ctatgataga ttgatagtga caaaatgtca cttgttgaat
    24361 agtgtaactc taaagtgaca atttgtcact tgtgttgttt ttttcttatc aataaattgt
    24421 ctaagacatc tttcgataaa accttaacat gagtcataaa aaatttattt aagccttatt
    24481 tatgctttcc atgataaact aaagtgacca aatcaatgag gtgggaaaat gattaaacta
    24541 ttagtagtag aagacgataa agtattatcc aataatataa aagaaatact cgctgcaatc
    24601 ggcgagataa cacaagttta tgatggtagt gaaggactgt tcgagttgga aagtggagtt
    24661 tatgatcttg caatcttgga tttgatgatg ccggaaatga atggatatga agtgttagca
    24721 aaaatacgga aagaaaaaat acagactcca gtattgattt tgactgcaaa agatagtttg
    24781 gaagataaga ttgaaggatt tgaacgaggt gcagatgatt atctaacaaa acctttctat
    24841 cgagaagaac tgatcatgcg cgtgaaagca ttattgaaac gctcgctagg tctctttgat
    24901 gaaaatgttc tgaagtataa ggatatcagt tgtgatttaa aaagcaatga agtcttgtat
    24961 aaagaacaag ttttgcccat acaaggaaaa gagtttgaat tgctcgttta ttttttacaa
    25021 aataagggaa tcattttgac aaaagagcag atatttgacc ggatctgggg atttgattct
    25081 gatacgacga ttacagttat tgaagtttat atgagcaact tacgaaaaca tctgcgaagt
    25141 actggattgg ataaagaaat caaaacctta cgtaatgtcg gttatatact gcaaagggtg
    25201 tgatgcaatg aatggagata ttacacgaaa gcaacgtagt cgcttttttg cgacaaatgt
    25261 agcagcattt gcttttatct tttttctttt gggattcatt actttacaga tattaaacca
    25321 atctgcatac agagaaacag atctgtcatt agagaaaata aattttgatt ctcgaacagt
    25381 acaattagag atacaaagat atcagcaagg cgatcccttt ttaaacccga ctcaaacgga
    25441 acaaaacaga acaaacatgg aacagccatt aatggaacca tcaaatgacc gttttaacac
    25501 tcaagtgatc ttgtggtcta aaaatggtga gatattgaat aaagcagcta ttggtgggcg
    25561 tttcaaccaa atcaaagaat taaaaatgga tacgaaaaat ctgaatacca tccaaaatat
    25621 tgaattaaat gaagacagtg gctcatctgc cctctctttc cgttcgatta cgaaaaaagc
    25681 ggagacgaat caagatggcg ttgcctacat ccaaatttta gaaaatacaa atcaaatcaa
    25741 gaattcatta gcgacttttc gcacgattat gattttgtgt atgattgttt tttggattct
    25801 ctctattggt atcagttatt acttgtctag tctaagcatg cgccctatta ttctttcttg
    25861 gagaagacaa aaagaatttg ttgagaatgc ttctcatgaa ttgcgcacac cgttaacgat
    25921 tattcaaaat agtttggaac atctttttac caagcctaat catactatta ttgaagaatc
    25981 cgaaagcatc gctcaagcat tgtcagagac gaggcgcctc actggattga cttcggattt
    26041 gctgacaatc gctcggaatg attctgatca gcagctgctt tcaaagcaaa tgatcaatac
    26101 acaagaatat atcgaacaac ttgttaaacc atttcaagag atggccatca tagatggaaa
    26161 agagtttatt ctccataacg atgcaacatt agaagtacca attgatgaaa aaaaaatcca
    26221 tcaagttttg gttattttgc tggataatgc attaaaatat acgaatccag aagataagat
    26281 cattttggat tcatctgtta accataaaga atggctgatt cacgtaaaga atactggccc
    26341 ttctatcagt gaagaagatc aagcaaggat ttttgatcga ttttatcggg aagatcaatc
    26401 tcgggcgaaa gagactggcg gatacgggct tggcttggca attgcaaaac aggtaatcga
    26461 acagcatcat ggacagattt ctgtatccaa ttatcttcca caaggagtcg attttaaagt
    26521 ccaattgccg ttgaagccta aaaaataatg cttatcccta aaaaagggct gtgacaaaaa
    26581 tcttgtcaca gccctttttt gttgcgaata aacggtgttc cagaagtagc ttcttcggaa
    26641 ataagccgaa atttctttaa gctgaccact tctgtcacaa cctctttagc ttacgcttac
    26701 gattttttat tgcggtcttc aatgtgtaca tcaccgcttg tgtcaatatc caattcttct
    26761 cggctaacat cttctgtcac tttttcaaca tcttcatgtt cttctttatg aatcccaact
    26821 tcttcacgaa taactgtatg tttgcttaca tctacttgct cttctttgat tgggatagta
    26881 atcgtgtcat cttccaattt ttcgtctgtt ccctgtgaag attgatctgt tactggtttg
    26941 cgttcgatga cgatttcttc acgtttgacc ggtacttcta ccgtttcagt atcgttgacc
    27001 acatgtttgt ggatgtcgac ttctccagtt gtcacatcat gtgtatttac atctaatttt
    27061 tcttctttta gttgaattgt atcatcatct agatttggtt tgcgatcgat gtcttcagtc
    27121 ttgcttgtac ccattgtatc agtctcgcca ggtacgtcca tcggatcagt tcctggtgtc
    27181 atattcaatg gatccgttgt attttgaaag cctgtcgtgc cacctacgtc cattggatct
    27241 gttccagtgc cagtttctaa tccagtcccc attgtgcttg ctgggctaac agtatcttgt
    27301 gtatccattg tatcttctgt tgtttctggc tgacgatagc cttttactgt gatgacaaat
    27361 tttccatcag aaatatctcc tctatattga tataaaggat cattttctgg tgtagccgtc
    27421 tcagaatcat aggtatcaaa agaaaaagta tcttttattt tttcccataa agaacggtct
    27481 tcttcaccat ccatccgatt ttcatctgtt tcaacatcta taccagacaa tccgtcaaat
    27541 cctagtctag atgctgcttc atcagtagta taaataacga tatcatctcg ttcataccct
    27601 tcgttacgca accgttcaat tactgataat gcttcttccc tcgtactata gcttccgatg
    27661 accgttaaat ttttctcttc catgtaaatt cctcctagca aatagattta tgattggta
//
LOCUS       DARBFO010000028        25832 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000028 DARBFO010000000
VERSION     DARBFO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.029"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            168..2228
                     /locus_tag="SCK51_001333"
     CDS             168..2228
                     /locus_tag="SCK51_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEG1251969.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2311..2880
                     /locus_tag="SCK51_001334"
     CDS             2311..2880
                     /locus_tag="SCK51_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1251970.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3055..3861)
                     /locus_tag="SCK51_001335"
     CDS             complement(3055..3861)
                     /locus_tag="SCK51_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1251971.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3999..5513
                     /locus_tag="SCK51_001336"
     CDS             3999..5513
                     /locus_tag="SCK51_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEG1251972.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5916..6899)
                     /locus_tag="SCK51_001337"
     CDS             complement(5916..6899)
                     /locus_tag="SCK51_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEG1251973.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6904..7551)
                     /locus_tag="SCK51_001338"
     CDS             complement(6904..7551)
                     /locus_tag="SCK51_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1251974.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7636..8268)
                     /locus_tag="SCK51_001339"
     CDS             complement(7636..8268)
                     /locus_tag="SCK51_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1251975.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8284..8835)
                     /gene="coaC"
                     /locus_tag="SCK51_001340"
     CDS             complement(8284..8835)
                     /gene="coaC"
                     /locus_tag="SCK51_001340"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEG1251976.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8832..9569)
                     /locus_tag="SCK51_001341"
     CDS             complement(8832..9569)
                     /locus_tag="SCK51_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEG1251977.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9566..10555)
                     /locus_tag="SCK51_001342"
     CDS             complement(9566..10555)
                     /locus_tag="SCK51_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEG1251978.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10813..11355
                     /locus_tag="SCK51_001343"
     CDS             10813..11355
                     /locus_tag="SCK51_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1251979.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11372..12457
                     /locus_tag="SCK51_001344"
     CDS             11372..12457
                     /locus_tag="SCK51_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1251980.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12469..13275
                     /locus_tag="SCK51_001345"
     CDS             12469..13275
                     /locus_tag="SCK51_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1251981.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13276..14103
                     /locus_tag="SCK51_001346"
     CDS             13276..14103
                     /locus_tag="SCK51_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1251982.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14100..15173
                     /locus_tag="SCK51_001347"
     CDS             14100..15173
                     /locus_tag="SCK51_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1251983.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15601..18297
                     /locus_tag="SCK51_001348"
     CDS             15601..18297
                     /locus_tag="SCK51_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1251984.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18374..18862
                     /locus_tag="SCK51_001349"
     CDS             18374..18862
                     /locus_tag="SCK51_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1251985.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18874..19368
                     /locus_tag="SCK51_001350"
     CDS             18874..19368
                     /locus_tag="SCK51_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1251986.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19389..20198
                     /locus_tag="SCK51_001351"
     CDS             19389..20198
                     /locus_tag="SCK51_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1251987.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20195..21016
                     /locus_tag="SCK51_001352"
     CDS             20195..21016
                     /locus_tag="SCK51_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1251988.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21067..23166
                     /locus_tag="SCK51_001353"
     CDS             21067..23166
                     /locus_tag="SCK51_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEG1251989.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23163..24281
                     /locus_tag="SCK51_001354"
     CDS             23163..24281
                     /locus_tag="SCK51_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEG1251990.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24320..25198
                     /locus_tag="SCK51_001355"
     CDS             24320..25198
                     /locus_tag="SCK51_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1251991.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25344..25541
                     /locus_tag="SCK51_001356"
     CDS             25344..25541
                     /locus_tag="SCK51_001356"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEG1251992.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         8176 a         4480 c         5441 g         7735 t
ORIGIN      
        1 aattaaaaag tacataagtt attattccga ttttcttgaa acaataaaaa atcaaaaaaa
       61 atactctcct aatagtccat cttcttttga ttttatttat ataatgaaat catcttatga
      121 aaattactac ttcatgttta gacaagagta gggaggacta agaataaatg tgttcaaaaa
      181 gacgttcaaa acaactaaat aaacacgctt gtcgtttaga taaggaacgg tcgtgttctg
      241 aaaaacacta cgttaagttt atgatgcgca cgatgctgat cagtttatct gtaggtgtct
      301 atatgcatgt acccgttcag gcaacgacac aaaccatcaa ggtcgtacgt acctctgttt
      361 ctgagaccac tgattcccct atcactatta ctcatacttt cactgatgca aatggaaaac
      421 agctacttcc tgcattgact tcctataata aagtcagtta tcctccaacg ataaaccact
      481 actggttaat tcctaacaaa acattatgga catcggaaac cggaagcagt gtgccgcttt
      541 ctatcttttc cgatatgact gatcctacaa aaggtgatca gttaaaacag atcactgata
      601 ttttgaatca tgctttgaca gatataaagg acggtggaag cgcacaagtt tcttatgtat
      661 atgaaaaaga ttattcacag tttgaaagaa agaaaatcga tgttctggca gggacaaaag
      721 tttcgatatc tgatctggta gaaaggctga agaacgcaga tggaacacaa ggagatatgt
      781 ccaaagtaat ttcaaacaat gggaatatcg tggatacgac taatacagga acatctacta
      841 tttccttgtc ctattttgat ccaattgctt taaaaacaat gactgcatct gctgtgatcc
      901 atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct tcacttaaga acaggagaga
      961 gttacgcccc taaagatctt gttgaatatg taacaaaaag cgatggaact tcaggaaata
     1021 aagaagaaat caaaatgaac gggacagtag atactacaac tccgggagaa tatcccgttg
     1081 tattaactta tatagactcg aaaacatcaa atgtggcaga aacaacagct gtagtttatg
     1141 tagaagcatc tgctgcttca tcgactgtca tcactcattt tatagatagt caaacaaata
     1201 aagaacttca agcagaaaca catatgagta gtgtaaatcg tattcctgat ttgcgcctac
     1261 ctgctgaatt ttctgatcga gtgccaaata tagatttatc gacttatgaa caaaatggga
     1321 aagtcacttc tatttctgat tggatgcaag caaacggatt agacagaaaa agtcactggt
     1381 cggagatcct tgaagtactt cactcagaac tgatgaataa taggaagcaa tcaggagggg
     1441 aaattacctt tacttatgtt tatataccag acaatcgttc attagaaggt catgatctta
     1501 ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat ccaatcagca acaaattcct
     1561 taggtaaacc cgtaccgtta aaggatgtat cggttacaat caatgaagta ccattggagg
     1621 atggttattt gaccaaacaa acaggtgatt ttacgattat gtatagttat catgacccgt
     1681 attcactcca agaactccat gctcagtcac tattacatgt tttaccatta aacaaacaga
     1741 tagatcaaaa agaacaaaaa agacgagaag aaagcttgtc agaaaaaaaa caacaaatgg
     1801 ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag aagcctagag ttagcaagaa
     1861 tatctagaaa aatctcagaa tctaataaaa cgataaggca agaagctcat ttggccccac
     1921 aagtttctgg attggaaaat gaaattttag agcctaaaat aaaacaacaa tcagaaaaag
     1981 aatttgttgc taaacagtcg gaagatcaga aggaatcaga tgaccagaag gaaacgaaga
     2041 aaaaacgctt gtcaactatt gattccaccg atgagcatct gcagccgcaa gtaccacttc
     2101 ctagtccggg aacacccgca cctggtggag gtattccttc ttctgcttta ggagatttca
     2161 tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa tgaatttgac gcagatgact
     2221 ggttctgatt tatatctcta tctgttatag tcaaataagt agctgagtca tatgttattt
     2281 ggcataatgg agacgaggag ggatagaaaa atgttagata aaacgttacc ttacgcagaa
     2341 atctggatga caagaccact tcaacaaaat gttccaatca cgcctttagc ggaaggtttt
     2401 gaatttcgat tttatcaaaa agaagatgaa tctgcttggg cggagattga gacttccgtt
     2461 ggggaatttc aaacgacaga tgaagcactt gagtattttc aacaaaactt tgctccttat
     2521 cctgaagaac ttgaaagaag gatgtttttt gtagagacaa aaaaaggaga aaaagtagca
     2581 acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt atccggtttt tcattggtta
     2641 gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag cattgactgc cagagtcttg
     2701 caagaatttc ctaccctcca tacggacgga ccaatctatc ttcacacaca gacatggagc
     2761 catcaggcga ttgctcttta taaaaaaatg ggttttactt ttattgctga aaatctagac
     2821 ggcacaccta atccagatta tgaaaaagtt atgcaggttt taagtaaatt agataaataa
     2881 cgaaatggtg aaataagtcc tcagtattgg gataaaaaac gaaatagtca ttcctcatat
     2941 cttacttttt tgagttaata ctatcggata actaatggca tgccatacat tcaaaaaaga
     3001 aggggcagtg acaaaagtat tgtcactgcc ccttctcttg ttttttagca tgttttatcg
     3061 tttgattttt ttaggaataa aattcttgat gaacgtaaat ttttggtaat cgtttcgact
     3121 atagaggtaa ccaaatacac tgaaaacaac tgctccaata aaattaacaa ataaatcctt
     3181 catagtatcc atgattccca cgtctaagta accattttgg atgattgttt tatctgtttt
     3241 accatctgca ttttgacttt caatgattgt ccgagtgata tctggaacat gatagacagt
     3301 gtttccgtct ttacttaact ccacactatt gatttgctgg atgatcgtat ctttttgcat
     3361 atcgaattgc agtaatttat ctgctgaaaa ttccacaaac tcccacataa caccaactgt
     3421 catcgaaaaa cagaaggaaa caactgctag taataaagga gtcaagttca tggtttttgt
     3481 atttttattg agcaaataaa caagggaaaa gccgacaccc ccagctaaaa atccgctgaa
     3541 tgcatgtaga aaagtatccc acattgaaaa atagccataa aaatcactta actctcctaa
     3601 aactgttgaa ctaaaaataa aagataacaa aatgatttct agcatatttg gaatctgtat
     3661 ctttaatatc ttttccagaa aataagggat ttcaaataaa attaatgtca gtaataaaag
     3721 cattgtattt ccccagtgtt ggttccagac ctgatccact agcataataa cgacgatgat
     3781 acgtaataaa atatagattc cgtttgtgat tctacctgca gtttttgtac tgttcgtaaa
     3841 aaaggttttc aaatgattca taaaataact ccttcatgta tatccttaat tatactaaga
     3901 agcgtgtgtt taccaaagct atatgactta aagaaaaaca gagttgatat tgtgaaaaaa
     3961 atcacttaat atagtataag gagaaaaggg ggatacaaat gaaaaaatta gttgctggtt
     4021 tattaacagc cgttgcactt cttgctatga gcgcatgcgg cgcagatcag aaaaatacag
     4081 cgcagacaag ttctagtacg aaggaatctg ccgaagtatc atcaggcgct tctgaacaag
     4141 tatatacaga tccaaaagaa atgaaagaat cttatgatgt cgtgattatt ggagctggtg
     4201 gtgcagggat ggcagcagct atcgaagcaa aagatgctgg gttgaatcca gtaattttgg
     4261 aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc aagcggtatg aatgcttcag
     4321 aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa tgatgccttc tatgaagaaa
     4381 cgctaaaagg tggtggagga acgaatgata aagaaatgct tcgatacttt gtcgatcact
     4441 ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa actggacaac ttaacgatca
     4501 ctggagggat gagtgaaaag aggacacatc gtccgtctga tggctcggct attggtggat
     4561 acttagtgga ggggttattg aataacgtcc atgagcggga aatccctatt tttgtcaacg
     4621 cagatgtgac agacattaag aaaaatgggg aaacggtcaa tgaagtgact gtacatgttc
     4681 aaggagaaga accaaaagag gtcaccggaa aagcaatcgt agttaccaca ggcggttttg
     4741 gtgccagcaa agaatttatt gaaaaatatc gtcccgattt agctgattat attacgacaa
     4801 atcaagaagg atcaactgga gatggtatca ccatgattga aaaattaggt ggacaaaccg
     4861 ttgatatgga taaaatacaa attcatccaa ctgtgcagca ggatgagggg gtcttgattg
     4921 gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca agaggggaaa agatttgtca
     4981 atgaaatgga tacaagggat aaagtatctg cagcaattac agcattgcct gaaaaatcag
     5041 cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac agctattgaa ttttatgacc
     5101 aaaaaggata tgtcacaact ggaaagacaa tggaagaatt agcagaaaaa atcgatttac
     5161 caaaagaaac actagcaaaa acagttgatg gatggaatac ggatgttgcg gagaaaaaag
     5221 atgctcaatt caaccgtgcc acagccatgg aacatgactt atcgtctccg aattattatg
     5281 cgatcaaaat cgcaccagga atccattaca cgatgggcgg tgtgaaaatc aatacgaaca
     5341 ctgaagtatt agataaagaa aaccagccaa ttagggggtt atatgctgct ggtgaagtca
     5401 caggtggctt acatggaaac aaccggatcg gtggaaattc agttgcggag atcattattt
     5461 ttgggcgaca agcgggcata caagcagctc gatttgcttc ggaaaatgaa tgaaatagaa
     5521 cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta ataccgaggg tcagctgatt
     5581 gaacactgtt tagtgaagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
     5641 caagtaaaaa cagcttctta tgtttttaac ttggttggac ttattaccgc agatgcaagc
     5701 ttttgaacac cgtttattcg gaaaaagagc gtgggacaaa agtaaagaac acttttgtct
     5761 cacgctctaa acacgtataa acggcggaag cagaagaaat tccttcggaa ataagctgaa
     5821 attcacaaaa atttgaaaag caattttcgt gaatttcttc ttatttcttg gagttaaacg
     5881 cttctgtccc agcctctttt ttgatcagca tgattttatt cagttatctt caaaatgatt
     5941 tttccgatgt ttttgtttgc ttccatatat tcatgcgctt gtttgactgc gctgaattgg
     6001 aatgtacggt caatgattgg ccgaatcttg ccattttcta gaaaaggccc agctttttgc
     6061 ataaaagttt gtgcgagctc tgctttatac tgatcacttc taggtgtcaa taatgtacca
     6121 agcagttgga cacgtttccc aatcaataca ccaaggtcta tctcaggcac gatggtccca
     6181 cctagcatac caatcaagac ccaacgtcca tcaacggcta tagaagctaa attcttttcc
     6241 cagtaagaag cgccgatgaa atccaaaata acatcgactc cccgaccttc agtgattttt
     6301 cgtacctctt cttgaaagtc ttgtttttta tagttgatca attcatctgc accaagttct
     6361 ttacaaaagg cgagtttttc ttctgatcct gcagtaacga tgattttcgc gtgagaaagc
     6421 tgtctagcta attggatcgc tgctgttccc acaccacttg ctcctgcatg gatcaagaca
     6481 gtttcgcctt cttgtaattt tccaagccag taaagtgttt gataagcagt caaaaaaact
     6541 tctgagattc cagcagcaga aacatagtct agcgtatcag ggagaaggat tgcacgatcg
     6601 gcaggcatca cagcatattc ggcataacca cctagattga ctaatccgta tacttttgtc
     6661 ccaggagtaa aagtggagat atcactttta ttctcaacta cctctccagc tacttcgact
     6721 cccaaaatag ggtagggagg tttcaaagag actgattctc ttttcataat atctgttcga
     6781 ttgacagctg ctgtatggac tttgatcagt aattcgcctt gttttggttt aggaacaggc
     6841 acttcaattt gttctaatac atctacagaa ccaggatgct tgattgaaat ggcacgcatg
     6901 gatctattcc tcctttgccc attcttggaa tgctgtattc aactctttgc taccttcaga
     6961 aagtactaga gtcgtacgag aaatatccgt ttcttccact agagcggcaa gtacagcagc
     7021 aactgtagag cgagtgatcg tttttgatgc cttctcatta ggcttagtta attgaatgga
     7081 agtaacttct tcatcgttag tcaatgtgac tggttgaaga atgacaaaat ctaagttagt
     7141 tcgtttcagc cattcatctg cataatgttt agtaatataa taatctgtca tggactcagg
     7201 ccattttgct ggttcatctg cataaacagc actgaccatc agatagcgag agacatgcgc
     7261 attttcagct gcgatcactg ttttgattgc gccatccaag tctacttgca gaagattctt
     7321 tccttgagag ccggcagcaa aaatcaatac atcgatgcct ttgaatgctt cggtcatttt
     7381 ttccacggac caagttaaat caaaagagac atacgaaaca ttctcttttt ttactaatgt
     7441 ttgggaagca acatctctca cccctgcaaa aatttcatgg gaacttggag ctaaatcttt
     7501 tatcaggtgt cggccaattt gtccgtttgc acctacaaca aaaattttca tttggatcat
     7561 cctttcgacg tcaaatcttt ccattcaata gtaacaccaa aagagaaaaa aatcagagat
     7621 cggctacgat gactatcagt tagtaagaga agggtgcttg aagtatctta acaagacatt
     7681 gcatactgct gcagtcgcaa gacctgcgac gattccttca ggtataccac ttgtaaaaat
     7741 aaccgctaga atagctgaat aaacagcatt taaatttgtt ccgatgactt tggcataatc
     7801 ttgctggaat aaaaagtaga tcatattcat cactaaaatc gtattggtca atgaaccgga
     7861 aagtccagct aagaataaag aaacggaatc tgctttttgt ttcattagtt tgcgcagacc
     7921 tttataaacg aaataaggaa ccaccccaat caagatgcga ggaataaacg cgatcagcaa
     7981 ggctttccag cttccatgtt ctgttccgat gactgggata aacggagaaa agacaaaaga
     8041 caaaggtgtt tggataactg tgctacggat catgctgatc agtccaaatg taccgccaag
     8101 aaaagcacct agtcttggtc ctaaaacgat cgaagcaatg attacaggaa tatgcatggt
     8161 tgttgcattg attggaccga tagggataaa acctaaaggc gtaacagcta ataaaatcaa
     8221 aatagctaaa aacatcgcag ttaatgtaaa gttttttgta tttttcataa gtactccttt
     8281 tttctaagca tttctttcat taagagtcag catgacctgt tcgatgatcg tctgaatatc
     8341 agctaaggca ccttttccaa aatctccaca agctaaaaga gattctcttg gttcaatttc
     8401 ctgatagccg acagatctca atgtttccag attacgctga gtgatcgggt tttgatacat
     8461 attcgtgttc atggcagggg caatcaattt tggtgttccc actggaagcg ccattgcgac
     8521 tgttgaaaga agatcgtctg caatgccgtt tgctagcttg ccgataatgt ttgctgtagc
     8581 aggcgcgatg agaaacaagt cagcttgttt tgctagttcg atatgattga ttacctgcgg
     8641 atttgattct tgcatgacgt ctgtatggac tgcgcgcttc gacagagatt gcaaggttaa
     8701 tggcgtgata aaatgtgtgc tgcttttaga cataatcaca tctacctggg cacctaattt
     8761 gacgaattga cttgtaagat cagcgctttt gtaagcagaa atacttccag taacgcctag
     8821 taagatcttt ttcattttga ttcctccagt ctaattttct cagtaatcaa tgcagcaatc
     8881 tcagatttac tttgagcttc ttccactgtt ccatctttag ataagagata gccgtggtgt
     8941 gtctcgccat ggatttccgt caaatcattt gcgaaaataa aatcagtttt gttatttatc
     9001 agactatttt ttgcgactgc cagaagagat tcctttggta catctactaa tagtttgaaa
     9061 ccaatgattt ttgcttttgg ctgccattca cgtagataag aaatgatttt tggtgttttc
     9121 ttcaaaacga gcacaagatg ctcggtgtta gaagagatct ttgaaacagt ggtatctgtc
     9181 tgttcatttt ccgcaaacca attgtccagt tcttcttgcg ttgaagaagt gggtaggttt
     9241 tttgccagct gttcttctga aaaactaaaa gcaggagtaa aatcactgac tgccatactg
     9301 tggataatcg catcgtagtg ttcttttttc gttagttctt ctaattgtaa aaataagtcc
     9361 agtgcagatt cgatcgtata aatcgttata ttggagtgtc ttttcggttt cagtgcttgt
     9421 ttcgtagtca cgtaatcgat tgtggtatcc ggattggcag ctaaataatc agctaatgct
     9481 tgccctaatc gtcctgtcga atgatttgta atagcacgta cttgatcgat tttttcagtc
     9541 gttcctccag ctgtgattag aatcttcatt tatggtcacc tacatttact agtgttcttc
     9601 cttggtgtgt tccagcaagt agtttagatg ctgtttcagg caattgatcc agagtaatct
     9661 cttggacgac cagttcatct gcaatctgca aatccgccat ctgttgccat aaagaaagac
     9721 gttgatctat tggtacattg acagaatcaa ttccaatcaa ctggatgttt cgcagaataa
     9781 aaggcaagac agtcgcgttt agtttgattc ccccagcatt gccacataaa gctactgcac
     9841 cgttataaga gatcaatgga agaagactgc ttagctgctc gccacctact gtgtcgatga
     9901 catagtcgat ttgctgtttt cctaaagctt ttgttgtctc tggcagaaat tcatcaggtc
     9961 gtacgacttg caaagcacct agacttttta gccatgtgac agactcttct tttctggaaa
    10021 aggcaatgat tgactggaat cctagtttat gtaacagggc tatcgctgta ctgcctacac
    10081 cgccagaagc accagtaacg acaacttttt tatcggaggt cataccttga cggattaaag
    10141 cgttcacaca caacatagca gtaaaccctg ctgtaccaag gatcatcgct tgacgcagcg
    10201 tcatattttt tggtagagga actaaccagt ctcctggtac tttttgatat tggctataac
    10261 cgcctggatg ggtgacgcca agcccaaaac cagtagcaag tacaagatct tcttttgaaa
    10321 aacgtggatt gttactttgg acgacagttc cggataaatc aatccctgga gtcatcggat
    10381 atgttcgtat cacctgacca gactcgctca ttgccaatgc atctttataa ttgacatcag
    10441 aataggcaat ttttaccaag acttcatttt cagccaactc agtaaatggc agatcactga
    10501 ttgctgtatg gaaaacaggt tcctttatta cctgaaaaga tcgaaaagcg ttcattcgtg
    10561 agacactcct tttaaaattt tcttttatca taccataaag cctactcttt tattcttgtt
    10621 cttccgtaaa tttagacaaa aattaggtga aaaataaaga atcaaacaac tagaatttat
    10681 tccaaaagat aaagaaatat tcagcaaaaa aaattttaca aagggttgcc tttttcaata
    10741 aatttcgtat actaatacga gtggtagtaa acaggaagtt tactattaga aaacttcgat
    10801 tcggaggaaa ttatggaaat tggtgagaag ttaagaaatt tgcgcatcca aaagaatttg
    10861 acacaagaag aactaggcga acgcacagat ttgtcaaaag ggtatatttc ccagttggaa
    10921 cgagatttaa gttcaccatc aatggaaaca ttcttttcta ttcttgaagt cttaggtgtg
    10981 actccagaag aattttttca tcaagaagca gcgaatctgc aagtggttta ttcaaaagaa
    11041 gatcatacgg tttatttcga tgaagaaaac gggtatgaat tggaatggct cgtggctgat
    11101 tcgaatgaaa aagaaatgga gcctgtcttg ctgacttttg aaggtgcagg agaatataag
    11161 acgtttgaac cttcattatc tgaaacgttt atttttgtat tagaagggga attagagtta
    11221 agcttaggag agacggtcta tgtggcaaaa gcaggacagt cgatctatta tcaagcgaca
    11281 aaacaacatc aattaaggaa tcatctcaaa aaacgaacaa aagtattgat tgttgcaacg
    11341 caatcatatt tgtaatatag ggggtaaaaa cgtggggaaa ccaatcattt catttgatca
    11401 agtagtgaaa cgctatgatg atgaaacagt attaaaaaaa gtaagttttg agatcgaaca
    11461 aggaaaattt tatacactgc ttggtccttc cggctgtggt aaaacaacga tcctacgtat
    11521 tatagctgga ttcaccgaag ctacagaagg ggacatctat tttgaaggga aacggttgaa
    11581 tgatttgcca gctaataagc gacaagtaaa tacagtcttc caagattatg cactttttcc
    11641 tcatatgaat gtttttgata acgttgcttt tggattgaag atcaagaaaa tgtccaaatc
    11701 agatatcgaa aagaaggtaa aagatgcttt gcgtatggtc cagcttcctg gatacgaaaa
    11761 acgggagatt agtgaaatgt ctggtggtca aagacagcgt gtagcgattg cacgagcaat
    11821 tgtaaatgaa ccgaaagtat tgctattaga tgaaccgtta tcggcgcttg atttgaaact
    11881 acgtacagat atgcagtatg aactgcggga tctgcaacaa cgattaggaa tcacgtttat
    11941 ttttgttact catgaccaag aagaagcgtt ggcaatgagt gatgagattt ttgtcatgaa
    12001 taaagggaaa atcgtccaaa gtggtacacc cgtagatatt tatgatgagc caatcaatca
    12061 tttcgttgct gattttgttg gggagagcaa tatcgtggac ggtgtaatga tcgaagataa
    12121 tctagttgag tttgttggta agcaatttga atgtgtcgat ggcgggatgc gtctaaatga
    12181 accagttgaa gttgttttac gcccagagga tttgacgatc acgacaccag ataaaggaaa
    12241 actagtcgta acggtcgata ctcagctgtt tcgtggtgtc cattatgaaa tcatctgtta
    12301 tgatgaacag caaaatgaat ggatggtcca ttcaacgaaa aaagctaaag aaggaagcaa
    12361 agtaggattg gcctttgagc cagaagatat ccatgtcatg cgtttcaacg agtctgaaga
    12421 agaattcgat gcacgtcttg acagctacga agaatagggg ggacaagaat gaaaacaatg
    12481 cgcaggatct attccgtccc ttatattttg tggctcggtt tattcgtgat cgcgcctgta
    12541 ttaatgatca tttatcaatc tttttttgat atgaatgggc aattcacgct gaataattat
    12601 ctatcttatt ttacgtcggg gacatcgctt tctatgacga tcaattctgt atggtatgca
    12661 tttttgatca cgtttttcac gctgatcatc agttacccga ctgcttattt tttaacaaaa
    12721 ctgaagcaca agcaattatg gctgatgctg attattttgc ctacatgggt gaatctgctg
    12781 cttaaagctt acgcatttat tgggattttc agtgtgcacg gcagcgtgaa tcatttcttt
    12841 gattttatcg ggatcgggcc gaaacagata ttattcacag acttcagttt tctgttcgtg
    12901 gcaacgtata tcgagattcc atttatgatt atgccaattt tcaatgcatt ggaagagatg
    12961 aatccttctt tgatcagtgc tagtagagat ttaggcgcga atagctttga gaccttccga
    13021 cgagtgattt ttccgttatc attaaatggt gtgaaaagtg gtgtccaagc agtatttatt
    13081 ccttctcttt ctttattcat gctgactcgt ctgattggcg gaaatcgggt gatcacttta
    13141 ggaacggcta tcgaacagca tttcttggtt acacaaaact gggggatggg ttctacaatc
    13201 ggtgtgattt taatcattgc tatgttcatc gtgatgctcc tgacaggcga gaagaaaaaa
    13261 ggaggtcgct cataaatgaa aaaatttaaa tggtcgtatc tttatttagg gatcgtcttt
    13321 ttattcttat atgcaccgat cttctatctg attttttatt ccttcaatga aggaggaacg
    13381 atgaacagct ttacaggatt tacttgggaa aattatgaag ctgtttttga agatacacgt
    13441 ttgatcatta tcgtattgaa tacgttcatg ctggctttct tatctgcatt gatcgccacg
    13501 atcatcggga catttggggc tatgggaatc tattacacga aaaaacgacg tgcacggaca
    13561 gcgttgttga gcatgaataa catcttatta gtctctcctg atgttattat cggtgctagt
    13621 ttcttgattt tctttacact actaggattc ggattaggtt ttcagagtgt attgctttcc
    13681 catattgctt tcagcattcc aatcgtcgtc ttgatggtct tgccaaaatt gcaggaaatg
    13741 aacgattcaa tggtcgatgc ggctagagac cttggtgcta ataatttcca agtagtcaaa
    13801 aatatcattt tgccttatct ttctccagga atcatagctg gatattttat ggcatttacc
    13861 tattctcttg atgattttgc ggttacattt ttcgtcacag gaaatggctt tacgacgctc
    13921 tcagtagaga tctattctcg tgcaagacaa ggaatcagtt tagaaatcaa tgcgctaagt
    13981 gcgttagttt tcttgttctc catgatattg gtcattggat actactttat tagtaaagaa
    14041 aaccgtccaa aacggctgaa gaaaaacaga agagtacgcg agggggtggc acggctgcga
    14101 tgaaaaaatt acaatctctg tttttaggta ttctcatcat tattgcggtg ctgctgcttg
    14161 gtgttcgtca acttgaaaaa acaagtggca tgagtggagc aaaggtactt accatctata
    14221 actggggaga ttatatcgat cctagtttga tcacgaaatt tgaaaaagaa tatggataca
    14281 aagtgaatta tgaaacattt gattctaatg aagcgatgtt cacaaaaatc cagcaaggtg
    14341 ggacaaacta cgacatcgct ataccaagtg aatatatgat ccaaaaaatg atcaaagaaa
    14401 aactagtact tccattagat cattcgaaga tcaaaggatt aaataatatt gatccacgat
    14461 ttttagactt ggattttgat ccagataatc agtattcgat tccatatttt tggggaactt
    14521 tagggatcgt atataacgat aaattctttg atgcaaatca aattaagcat tgggatgatt
    14581 tgtggaagcc ggaattaaaa gacagtttga tgctgattga cggtgcacga gaagttatgg
    14641 ggctttcttt gaatagttta ggctattctt tgaatagtaa gaatatggca cagttgacag
    14701 aagcagcaaa taaattgaat catttaacac caaatgtcaa agcaatcgtg gcagatgaga
    14761 tcaaaatgta tatgatcaat gaagaagcct ccgtggcagt gacattttct ggcgaagctg
    14821 ccgatatgat gtgggaaaat gaacatttac attatgtgat cccaccagaa ggatccaatc
    14881 tttggtttga taatatcgtt atgccaaaaa ccgtgaaaaa taaagaaggt gcctatgatt
    14941 tcatcaactt tatgctagaa ccggaaaatg cggcacaaaa tgcagagtat attggttatt
    15001 ctacaccaaa tgaagaagca aaagcgttat tgccaaaaga catttcgtcg gacgaacaat
    15061 tctatccatc tgatgacacg atctcccatc ttgaagtcta caaagatcta ggatcgaaat
    15121 acctaggcat ctataatgac ctattcttag aattcaagat gtataggaga taatggcaaa
    15181 actcagtaag aatggggact tatcaacgtt ctaaagagct aaaaatcgtc ctttttctat
    15241 ctgagttttc caatcttcta ccaaaagcgg atgaaacgcc gctttttttg tgttatttta
    15301 ttttttctac cacgtttttc taccagagaa aagtggacaa aaccattgta aaatagggcc
    15361 cgagacgggc tagtaaatct aagattgaaa aagttacaga aggataaagt aattggaaac
    15421 tatgcaaata tacttaaccc ttctgtaatc tattaataaa actaatatat gaatccagtt
    15481 caatatttgt agctatgaaa aaacataaaa atcatattag ataaaaacta aattggtaga
    15541 taaattattt tgtcagatat ctgaggcaaa tattcgacta tttgtgtttc aaagtaaaaa
    15601 ttggaataca aatatagaat agacatattg cagccgatag tttattatgc ttttagacac
    15661 attaaactgg aggaaaaaat gaaaaagagc gctaaagatc gagtttttga tgagctgata
    15721 cgactaacag ggaatatgtc ttctgtcact gccgagaaaa tttcgaagaa tctaggtatc
    15781 tctcgtcaga atgccagcca ttatttgacg agattagtgg aagataaaaa ggtcgagaaa
    15841 ttaccgggaa agcctgtttt gtggaagcct ttagatgaat atgcggttgt agataatacg
    15901 gagcaaataa acgaagcctt tcattctgta gttggtcatg acggaagtct gagagaggtc
    15961 attcaaaaat gtatagccgc tgtcaaatat ccgcctaacg gactcagtgt attaataaat
    16021 ggagccacgg gtgttggaaa aagtttttta gccacaaaaa tttttgaata cgctatccat
    16081 gagcaaatta ttgaaaaaga tgcaccattt gctatcttaa actgtgccga ttatgcggac
    16141 aatcccgaac tgctttcggc aacactgttt ggatacaaga agggggcctt tacgggggct
    16201 gaaaaggata ccgatggttt attggcaacg gccaacaacg gctatctttt tctcgatgag
    16261 gtccatcgcc tatcgaaaga aaatcaagaa aagctctttc tatttattga tactggaaac
    16321 tatcgtccag tcggtgaaaa tgtaaattgg cattctgcta aggttcgttt tattttcgcc
    16381 acgacggaaa aaggtgaaaa ttatttatta gacacctttg atcggcgtat tcaaattagt
    16441 gttatgttgc caacttttga agatcgcccc attcgagaac gattagagct gattcaatta
    16501 ttttttcaaa atgaagcgga tgttttacaa aaagatatta ttgtttcaag agaagccttg
    16561 tctttaatgt tatctcatcc ctttagtggg aatattggta agctgaagaa tatcattaaa
    16621 atcagttgtg cggatgtgta tagtcgaaca caagaagaac cattaacgat tgggaaaaat
    16681 gagatcttga ttcagttaaa tatgctggaa tccgaagtag aaagcttgcc gattgctagt
    16741 ttattgataa agaaaaatca aaagataaaa acggatcgaa tcaaaccgtt tgattatgat
    16801 tcgattttgg aaaaaattac tcagctgata aatgaaggta atttcaatga aaatttttct
    16861 tcgataaaga atgaagtaca gcatttggtg catcacattg ataaggattc ttttttatca
    16921 atgagtagaa aattgatgat tcagatgttt agaaaggtct gggtatccgt tttaggaaaa
    16981 acgtacggat tgacgacgag tggacctgtt gcagaaatta ttagcagatc ctatgtcatt
    17041 tccccaagct ataagacaga cttaaccggg ataaatcgta cattagctgc aaatctacct
    17101 cggacgactt acttatgtaa tcagttcttg gagcatttac caccgctctc agaaaaggac
    17161 gagaactttc taagactgct tattgtcgtg actttatctg attatgtaga tgaatccatc
    17221 gagctgaagg gcctgttgct tgctcacgga gatagtacgg ctagcagcat tcaagcagta
    17281 gtgaatcaat tatgcggcaa ttatgttttt gaagcgttgg atatgccagt agagagtaat
    17341 gtgtcagaga ttgtcgaatc gacaaggaat ttcgtgaaac ggcaagtgaa tacagatagt
    17401 ctagtgctga tcgtagatat gggctcttta aatcaaattt acacacagat caagaacgat
    17461 ttaaaagggg agttacttct tctgaataat ttgacgactt ccattgcact tgatattggc
    17521 ttgaagatga cgatgaatgc gccatttaaa cagattgcgg aggatgcagc agacaagtat
    17581 tcaatcaatg ttcaatactt tgaaggcttt tctcaaagta gcaacattat catttcctgt
    17641 atgtcgggtt tggggatttc ggacaaattg aaagatgttt ttcgtcatca tttatcggat
    17701 gaatccatcg aagttttcac gaaggactac agagaactac gtgaattaat cgaccaaaat
    17761 gatgaaactt attttgacaa gacaaaattg gtcatcacga cttcggatct tccacgttcc
    17821 ttttcaattc ctaatattaa tgtttatgac atcttagacg gtgatggagc agccaatttg
    17881 gagcatattt tgagtcctga aatgaagccg gcagaattta accgcttgat tcaggagcta
    17941 gtaaaattct tttcgttgga aggaattgcg gaccgtttga actttctgaa cccaacgatc
    18001 gtgatgagtg aagtggaaac agtcatttct aaatatgaaa attactatca tttcacttta
    18061 aatggccgtg taaaactaaa tctctatatg catacggcat tgatgatgga acgtttattc
    18121 ttggctcgga cagaaaaatc tgaagaaccg aattatgatt tgaaaccaga tgaagatgaa
    18181 ttttattatg tttctagaag tatttttcaa tcaatggaga tgaaatataa ttttaaagtc
    18241 aatgcttatg agctttcgct gctctatgaa ttattaggac cgtatattca aaaataaagt
    18301 gtctaaaaaa gtgtctaatc tgtttttttt aggttagaca ctttttttaa tgtgaattaa
    18361 cgtgattaaa ccaatgtttt taaaagttgg cacaggaatt gcttatataa aagtgaacag
    18421 aaagaaagga attaaccata tgaagaaaat tctaatagcg actcacggtc acttagcttc
    18481 aggctttctt agcagtattc aattactgac ggggaaggtt caagagatta cggtgattaa
    18541 tgcttatgtg gatgagtgtg attttgaaca ggagttgaat gtatttgtaa accaggtgaa
    18601 cgagcaggat cagatttttg tttttaccga tttatttggc ggaagtgtca atcagaagat
    18661 taccaaaacc tttttggaga aagagattgc tgtaactatt ttgtctggct ttaatctgcc
    18721 aattctgctc gaaatagtat tggcaaccaa ggaattaaca aaggaggata ttcaggaact
    18781 ggtaaataaa tgccaacagg aattaaagat gaccattctt gatgaggtac atgtagtaaa
    18841 tgaagaggaa gatttttttt aggaggaaga aaaatgatta aagcagtgcg aattgatgaa
    18901 cgattaattc atggacaagt agcgatgact tggacaaaga gtctaaattt aaccgggtta
    18961 gtagtagcaa gcgatgaggc ggctaccaac gatattcaaa agatgacatt gaagatggct
    19021 gcaccatcga atgtcaaagt cattattaaa acagttgatg acgcgattca tttgttaaaa
    19081 gatccgcggg cgaaggacat gcgcttgatg gttttagtcc ctaatgtcaa agatgcggta
    19141 aaagttgcga aagctttccc aacggaaatc gaattaatga acgtggggaa tgctgggaaa
    19201 atgacaccgc catcggaaga taagaaaacc ttatcaaagg aagtgatgtt aacgacgtca
    19261 gaaattcaag acttaaaaga attagttgaa ctttatccag ataccgtttt tcagcctaca
    19321 ccggcgatgg aaaaacgttc agctatctct attttgagtg atttttagaa aaaggaagga
    19381 gaaaaattat gtatgatgca tttgtagtcg ctttggccgt ttttattgga attgctggac
    19441 atgaaatgtt cgggatgtcc atgctgagtc gtccgattgt tgttgctccg ttagcaggag
    19501 cgttgatggg ggatttgcag acggggttga ttgtcggggc gtcactagaa gccatcttta
    19561 tgggtgtcgt caatatcgga atctctagta cggcggagcc ttctttggct gctgggctgg
    19621 ctacagcgtt cgcaatcaaa atgggaggaa acgtggatgc gattattccc attgtctttc
    19681 cactagcagt attaggatta cagttaatga atatgatttt ttcttttgta tgtggaccga
    19741 tggctgctaa atttttcaaa tttgcaaaat ctggtgatca aaaaggaatc atcaatctgc
    19801 acttctttat gtggtttgtc cactatgcgc tttatgctct tattccattc tttgccgttt
    19861 tatttggagc agatgttgtc aaaagcgtct tggataatat tccagaggtc atcatgaacg
    19921 gattaacagt cgctggaaat ctgctgccgg cagttgggat ggcgatgctg ttacggatgc
    19981 tttggagtaa aaatattgct gtgtggtttt tctttggggc cgttgttatg gcgtatctcg
    20041 agttaccatt gattgcaatt gccgcagttg gaacgattat tgcaattgtt atagcccaaa
    20101 gagatctaca actaaaaaat atatctgttc atgcagttca aagcgatgct gctgtaaatg
    20161 caacgcttga ccaagaagag gaggatttct ttcaatgaaa ttagctgatg gaatttctaa
    20221 agatgagaaa aaattggttc ggaagatgtt ttggcgctcg atgactatgt atgcttcggt
    20281 gaatccggtg acgatgggcg gcggtggttt cgcctattct atgatgccct tcattaatca
    20341 tttctacaaa agtgaagaag atcgagctgc ggcgctggaa cgtcatacac agtatttttc
    20401 gacgaccatt ccttgcgcgt cctttgtcat ggggattgcg gcatcgatgg agaaggaaaa
    20461 ttctgaaaaa gctggttttg acgtggaaag tatcaacgcg atcaaaacga gtttgatggg
    20521 accattggct ggaattggcg attcattgtt ctggggagtt tggcgtgtcg ttaccgcggg
    20581 actagcgatc aacttagcca atgccggaaa tattttggca ccaattatct ttttagttat
    20641 gttcaatata cctaattact tgatgcgata ctatggcggc gttttaggct attcattagg
    20701 ctctaaatat attgaaaagc tgtatgcgaa cgggttaatt gaaattctga caaaagctgc
    20761 tagtatcttg ggtctgatca tggtaggtgc aatgacgatc agtatggtca aattcaatac
    20821 cgttttgagc ttttcaatga agggcgaaga actaatgaat gtccaagaaa tcctcgatag
    20881 tatttttaaa ggaattgtac cgttattggt aacattgagc tgtttcaaat tactagataa
    20941 aaaagtcggc gtcatcaaaa ttatttttgg aatggtcgtc ttgggaattt tattgtcatt
    21001 acttggaatc gcatagtagg aagctgcagc tactaggctg cagttttttt agcaaggagg
    21061 gaaaagatga ctattaatga agtaatttat tcacccaaac acccattttt taatgctgag
    21121 ttaaacgacg gaaatgtgac tacttcaaac gaatacagct actatcggaa tattcctgat
    21181 gtaaagggat tgaccttcga tgtaagtaat caaaagacat tagcaaacct tgatgtttgc
    21241 gggacgattc aaacgttgac cttttatcga gaaaatttac taactgagga aaaaccaggt
    21301 gtttgggtca ataaacagct ctctcaaacc aattcgttat ccttaaaggt tgaagtaaac
    21361 gggattgtct accatttgtc agaatcggat catcgtattg aagtcgatgt gattcaagac
    21421 tgtttgccta gatatattca tcattattcg acgtttaagg tcattgtagt atcctttgct
    21481 cccatactgg ctaataagcg tttaagtatg cttgttcaac aggtttacat tgtgaatgaa
    21541 acacacgaaa gccttcaagt ttcagtgaaa gatgtgcctt cgtatcaaga gaagtattcg
    21601 gatcagcaaa atgtgttgat ttctcaaaaa ggaaataagg acacgatcgc acaacaggaa
    21661 gtggcaagct tcgccattgc ttttgtggac ccaaatgctt ttgaagaagt aatgactttt
    21721 caggaatctg atatcgaaaa atggctgaag gaaacattga actattttgc tcagctattt
    21781 gggcagctgt caatgccaaa cgatagagtt gtccatctct atcgtcgtgc actttatcaa
    21841 agcttttcta gtttcggtat gaatcgacag ggacaggtcg tcggcagcaa ttggggcagc
    21901 tatcctgcta ctaatcgaat ttggaataag gatatgtatt acgcatcatt gccattcacc
    21961 atgtttgaac cggagttatg tcaaaaaacg gttctatggt ttgatcaata tggcgttaag
    22021 tttcctggtt caaaatttac aggaggtatc aatcattctc tgagtaattc gttatccagt
    22081 gcgttactgg ctagtcttta ttttgaacat actggggata caaaattctt tgagcggtgt
    22141 ccgcaggttc tactgaatgc ttcaagaatt attgaggaca tattagctca acgtcggtca
    22201 ggggaaccaa tgctttttcc atctgtttgg ttatcagacg cctttgcact tggaaagtat
    22261 cataccggca gcaatctttg tctttggaag gcctgtattg gacttgcaga aatttttgaa
    22321 gccctagaac aactgagtct tagcaagcga tacaaaaaca ttgcctgtaa actaaaggaa
    22381 tcaatttgta aacaaatgac tatcgatggt ccttttggtg agcaattttt ggagggaatc
    22441 ggtgatgagg agaaaacaac ctattcagtg aagcactatc aaaaaccgat tcttgagcaa
    22501 gggttaatct ttttaagtga tgtgattgtg gatggaaaaa ttaatttatt gatgcatgat
    22561 ggagaagaat cagacactac gttgatgcct ttttatcaat ttttagataa taaagatcat
    22621 ttatatcaga acaccatggc cttttctgct agttctttca atccaaccta tagcgaggaa
    22681 atcaaaggca tcacttgggg attagaatcc ggcgccactt ttcctggctt tatcactgtt
    22741 ttaatgagcg agctgcacaa ccctcaagct ttcgtaaccc gattagaaga actaatttgt
    22801 ttggcagatt tagacggttc gtggtggtgg tggccgtatc gcttagaggc tcaacaagga
    22861 gaagtcgtgc gagactttgg ctgcggcaaa tgcggctggg catcgggtct ttttgtcagc
    22921 ctaatgatca gtcaatattt cggattgaaa ttcaaaaaag gagtattgca aattgatcct
    22981 gttgataatt taacgttttc ttggaagaat ttgaagttcg gtcaagcctt catttcgatt
    23041 gaatgtacgc aatccgagtt gagtatttca aatttgtcag aaaagccatt gaaaataagt
    23101 gatacgaaaa aaattcttca cgtagaaaag ggaaaaacga ttcacattca aaggagaaaa
    23161 agatgagtat actaaaaaat attacccaat ttaaaaaaat ccagagtcga gcaatcactc
    23221 ccgaaaatcc aacaggcgag aaaggtaaag ctgcgatgtt gccaagcgag ctaggaccaa
    23281 gccgcaaagg tcgtggcgca attccgctga cttctggtga agaaactgtg attgcggata
    23341 ttactggaac gggaataatt cgtcatatgt ggatgacgat tcgtgatcga acagaatttg
    23401 gcagttttgt tttacgtgat ttaatcttaa gaatttattg ggatgggtcg gatagccctg
    23461 ctgttgaaag tcctttaggg gatttctttt gtaatgggtt tggagagcgt tgcgaggtga
    23521 actcgttgcc gatagtggtt aatcctacag ggggctttaa ttcctatttc gaaatgcctt
    23581 tccaaacatc cgctaaaatt acgatcgaga atttacatcc gaaggatatg tcctcatttt
    23641 tttatacgat aaattacgct ttggtggacg aattggaaga aaatagccta tattttcacg
    23701 caaattggca tcgggaacgc attaccaaat tacaggagga ttataagtta attgatcgat
    23761 taaagggtca aggctattac gtgggtactt accttgcgct tacctgccta gaacgttatt
    23821 ggtggggcga aggcgagttt aaattctaca ttgatgggga tagtgagtat ccgaccgtaa
    23881 cctcaacagg ttcagaagat tattttggcg gggcgtgggc ctttcatgag tacgatgagt
    23941 tcggacgtcc acatgcgaaa aactattcaa cagccttttt agggtatcct taccaatcaa
    24001 atagagatgc cacaagagat cgttttgaaa cgggaaaatt gaatgccgtt catgcctttg
    24061 gtgacgacgc aatgccacga catggtcttt atcgttggca tcttcaagac cccatctgtt
    24121 ttaccgaaga tttatcggtc actttacaac aaatcggcaa cgatgatgtt cgcttatttg
    24181 agcggcaaga cgatgttgct agtgtcgctt actggtattc aacagaagct tcaggaaata
    24241 ctaaacgtca tttcactgat cgagaacgct tgccgcgtta atgaagtgtt tttcacatat
    24301 tcgaagggag gaacctatga tgatatatgg ggcaattgaa gctggaggga ctaaatttgt
    24361 ctgtgcagtc ggtactgagg aattaacgat tttggaaaga gtcagttttc ccacaacaac
    24421 tccagttgaa acaatgaagc aggtcattga tttttttaaa ccttttaagg aacaattggc
    24481 aggcatcggg gtcggctgct ttggtccaat tgatattcaa tgcaaatcaa cgagtttcgg
    24541 tcatatcaca tcaacaccaa aacttgcttg gcaaaatttc aattttgtag gaacgctaaa
    24601 gcaatatttc aatattccga ttgaatggac aacagatgtc aatgcggctt gctatggtga
    24661 atatgttgct ggttctggca aagggttagc aggtgttgca tacttcactg tgggaacagg
    24721 tatcggaggt ggtgccttgt tcaatgggac tttcgtggaa gggttcagtc atccggaaat
    24781 ggggcatatg ttgatcaagc gacatccaaa ggacaatttt tcaggaaact gtcccttcca
    24841 ccaagattgt ttagagggga tggcagcagg accagcgatt gaaaaacggt tgggtgtaaa
    24901 aggccagaat ttattggctg acgattcgtt ttggcagatt gaagcctttt accttgccca
    24961 atgtgcatac aatacaacac tgatgttttc gccagataga attatttttg gcggtggtgt
    25021 aatgaaacaa gaacacatga agaaaaaagt tcaggataaa tttgttgagc ttataaatgg
    25081 atatgttgaa ataccaccaa ttgatagcta cattattacg cccgaattag gtgataatgc
    25141 cggaataatc ggcggattag cattggccag aaaagcggta agaaataagc aaccataatt
    25201 aatgaatgtt tcattgatac tcatgtcaaa agtatttaac cgagtaatca aatgatcaaa
    25261 tgaagttgga taaagcgaaa atgaattcaa ataaaaaggc tctaagtcaa atcgtgttgt
    25321 tttcattaaa tcactgaaag gaaatgcttc tgcgtttcct tttttctttt tgttgtgcgc
    25381 tgtatacctt gaggatattc actggtggcg tacacttaaa tagtaatata ttgtgttact
    25441 ttatgtctgt ggtaatagtt atagttgctt accaatgtat gttcattttt cgaaatgttg
    25501 acttcataat ccttggctta atgtttgaat ttttttggtg atagattttt tcagccgtat
    25561 aaaataaatc gtaattctat tatagagagt tgtgattata aaaagatagc aagaaaaaaa
    25621 aagaaagttt ctatacattt taaatatttt attcctattt ctattgctaa aaaagtagaa
    25681 attctatatt tacctttaac tctcgcttct cttgcaaatg tagttagcat tttaatcgat
    25741 aataaactca ggtagttata ttaccttgaa atgattgata attttgtcac ttctattttt
    25801 tttgcaataa attttttatt ttagtgaaaa at
//
LOCUS       DARBFO010000029        25495 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000029 DARBFO010000000
VERSION     DARBFO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.030"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            127..3654
                     /locus_tag="SCK51_001357"
     CDS             127..3654
                     /locus_tag="SCK51_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEG1251993.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="SCK51_001358"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="SCK51_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEG1251994.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="SCK51_001359"
     CDS             7442..7783
                     /locus_tag="SCK51_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1251995.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="SCK51_001360"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="SCK51_001360"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEG1251996.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="SCK51_001361"
     CDS             9140..11560
                     /locus_tag="SCK51_001361"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEG1251997.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="SCK51_001362"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="SCK51_001362"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEG1251998.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="SCK51_001363"
     CDS             12994..13731
                     /locus_tag="SCK51_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1251999.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="SCK51_001364"
     CDS             13745..14560
                     /locus_tag="SCK51_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEG1252000.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="SCK51_001365"
     CDS             14573..15220
                     /locus_tag="SCK51_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1252001.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="SCK51_001366"
     CDS             15236..15892
                     /locus_tag="SCK51_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1252002.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="SCK51_001367"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="SCK51_001367"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEG1252003.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="SCK51_001368"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="SCK51_001368"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEG1252004.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="SCK51_001369"
     CDS             18234..18752
                     /locus_tag="SCK51_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG1252005.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /locus_tag="SCK51_001370"
     CDS             18859..19359
                     /locus_tag="SCK51_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1252006.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19639..20559)
                     /locus_tag="SCK51_001371"
     CDS             complement(19639..20559)
                     /locus_tag="SCK51_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEG1252007.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20724..21737
                     /locus_tag="SCK51_001372"
     CDS             20724..21737
                     /locus_tag="SCK51_001372"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEG1252008.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="SCK51_001373"
     CDS             complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="SCK51_001373"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEG1252009.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22822..23262
                     /gene="fabZ"
                     /locus_tag="SCK51_001374"
     CDS             22822..23262
                     /gene="fabZ"
                     /locus_tag="SCK51_001374"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEG1252010.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23377..24138)
                     /locus_tag="SCK51_001375"
     CDS             complement(23377..24138)
                     /locus_tag="SCK51_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG1252011.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24327..25421
                     /locus_tag="SCK51_001376"
     CDS             24327..25421
                     /locus_tag="SCK51_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEG1252012.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8448 a         4424 c         5382 g         7241 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
    19501 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
    19561 ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttta gtgagattcg
    19621 ttgcatcgtt tgtccaaatt attttccgac agcaattgga ctgttcggaa tcgtgaaacc
    19681 agaagctgtc aattctttga cataggagct caagaactct tcttttaagg catattgttt
    19741 tccgttcaat gcgtagcaga ccgtacgaat cgcaaaattg ctgtttccaa gatccactaa
    19801 tccaaagata gaaggttcag tttggagctc ttcttggtat ttttcagcta atgtacgatt
    19861 tactcgatca atgatctcat agattttgtc atatccttct tcaggaacaa tccgaatatc
    19921 taaaagtact tgcatatttg cacgagagag attgctgatc gtggtgatgt ttcgattcgg
    19981 gatgaaatgg acagtaccat ccgttgcttt cagctgaagg gtacggatac caacagatac
    20041 gacggttcct tcaatcgtta agtttgctag cttgatgtag tccccaacat ccatctgttg
    20101 ttccatgata ataaaaaatc cggtaataac atcactcatg aacccttggg caccaagacc
    20161 gattgccaca cctgcaattc cggcaccagc caaaagtgat ccgatcggca caccgatagc
    20221 agataaaatt gcatagataa aaaagaaacc aagggtataa tgaaaaatag tatttaaaag
    20281 actatgtaaa gtctttaagc ggctctcgtt tagaatggct tttttagaat actgattaaa
    20341 agacttatca atcagatatt ttcccacacg gtgaagaatc caaaaaagga cgatcaaaaa
    20401 aatcagatat aaactttttt ggataaaggc agccaaaatg gcgtcccaat caatcccgtc
    20461 ccaaaaccgc tgaaacgcat ttactttgct gacagcttga tctgtcaatg tctcgccaga
    20521 agagtctgtc gtaccggcag ttgctaataa tgaaaacata aaatcctctt ttctaaaatt
    20581 tgattctttc tgtattgtac caagaagttt tcaaaaaagg aaatagcaaa aataactata
    20641 atgaaggaaa aaagctgaaa ataaaatgac aaataatatg gataatgata aaatgtggag
    20701 aaaataacga aggtggtaag tatatgcaaa tcgattggaa caatctaggt ttttcttata
    20761 tcaaaactcc ttggcgtttt attgccaaat ggaaagacgg acaatgggaa gaaggagaat
    20821 tgacagaaga taattacctg acgattcatg aaggctctcc agcgcttcat tatggtcagc
    20881 aatgttttga aggtctaaaa gcttatcgtc gaaaagacgg caaggtcaat ctctttcgac
    20941 ctgaacaaaa tagtcgacgg atgaatcaaa gtgctagacg attgctgatg ccaatggtcc
    21001 ctgaaaagat gttcgttgat gctgtcaagc aagtggtaaa agcaaatgaa gattttgtgc
    21061 cgccctatgg aacaggcgct acattatact tgcgcccttt actgattggg gtcggtgaaa
    21121 acattggtgt ccatccagct ccagaatatt tatttgtcgt cttctgtacg ccagtaggag
    21181 cgtatttcaa aggtggacta aaaccaacga attttattgt atctgattac gatcgtgctg
    21241 ctcctaaggg gactggtgca gcaaaagtcg gcggaaatta cgcagcgagt ttacttccag
    21301 gaacagaagc aaaagaactg gattactcag attgtgttta tcttgatcca gccacacata
    21361 cgaaaatcga agaagtaggt gcagcaaatt tctttggaat tacaaaagac ggaaaattca
    21421 tcactccgca atcgccctct atcttaccaa gtatcacgaa atattccttg ttgcagttag
    21481 ctgaagaacg actaggactg caagcaattg aaggagatat tttcattgat gatctagatc
    21541 aatttgcaga agctggtgca tgtggtacag cggctgttat ttctccaatc ggtgggattt
    21601 atcacaataa caatcttcat gtattttata gtgaaacgga agtaggacca gtaacaaaac
    21661 ggttgtatga tgaattgaca ggtattcaat ttggagacat agaagcacca gaaggctgga
    21721 ttgaagtcgt ggaataattt caaagtaatg agttgaaaaa aacgtaaaaa gaaaaaatca
    21781 ggaagagact ttaaaaagtt ttttcctgat ttttatatgt tttactttaa agaatgcaac
    21841 aaaagtttat gaaagatgga cacctttgtc aacgtagata atatcaccga tgattccgct
    21901 ggaaagtggg ctgattaaga aagcacaagc attaccgact tcttcgatcg tcacaccttg
    21961 ctgatcaggc gtacgagatt cagataactg gatcagtttt tgatagtctt taacacctgt
    22021 gacagctaat gttttgattg ccccagctga gattccattg acccgaattc ctttaggcga
    22081 gaactcagct gccaaataac ggatagcagc ttcaagagaa gctttcgcaa tacccatcat
    22141 gttataattc gggattgcgc gttctgaacc tagataagtc atgctaacga tcccgctgtt
    22201 tggtgcaaga aacggttgtg cgtattttga tacagcaatt aaagagtagc tgctgatatc
    22261 ttgagctaat tgatagccgt ctctggaaat atcagacaca tttccggaca attcttcttt
    22321 gttggcgtaa gcaactgcat ggacaaggcc gtggatctcc ccagcatatt caccgatggc
    22381 attcattgct ttttcgatgc tttcgtcgct agaaacatca cattctacag tgaatgcttc
    22441 ttcgccgacc aatttgacta atgatttttt cattcgttca ttttggtaag tatagataat
    22501 ttctgcccct tgtttcaaca ttgcttcagc acaaccccaa gcaatgcttt ttttgttagc
    22561 gacgcccata atgacgattt tttttccttc tagaaatgac ataatgactc tcctttgttt
    22621 ctgataaaaa tttgatagtc gtaaaaaaat agagaataat agctaaaata acttcttgat
    22681 ttcttaatta ttagtcaaaa tgttttgatt gtcaaactat tttttcttaa attaaaaaaa
    22741 gattgctaaa tatttaggaa tatgatactt tgatagtcaa agtaatgaaa atggcaattg
    22801 cctgaaagaa ggagtttaat tatgtcttac gtattatctg ctactgaagt aatggaattg
    22861 attcctaatc gttatcctat ttgttatatt gattatgtag atactttaga accaggaaaa
    22921 aagatcgttg cgacaaaaaa tgtaacaatc aatgaagatt ttttccaagg tcactttcct
    22981 gacaatccag tgatgccggg tgcgttgatt ctagaaacac tggcacaagc aggttcgata
    23041 cttatcctaa aatcagaaga gtttctagga aaaagagctt atatcggtgg gatcaataag
    23101 gccaaattcc gtcaaaaagt tgttccagga gatgtgctga aatgtcattt tgaaatcacc
    23161 aaaatgaagg gatcagtcgg tacagcgtta tctgcagcat atgtagatga taaaaaagtc
    23221 tgtgagtgcg agttcacgtt catcgtcaac caaaatgctt gactataaaa cctgatatag
    23281 caaaacaaaa ggaagcttac ttgcttttta tgtaaaaaga agacgcctga atcctagcat
    23341 acgttgtcat ctatgcagga ttcagacgtt atttttttat ttttgttttg cttcttgcag
    23401 tatattgatt ttatcgattt ttacttcttc ttttggtttg tctgaactat ctgtttcgat
    23461 atttgcaata gcatccacga catccattcc ttcaatcact tggccgaaga cagtataatg
    23521 gccatctagt gaaggataac cgccgttttt ataagcgtcg atgattgctt gaggatagtc
    23581 gtcttttaaa agaccatcat gcatatcatc tgtattttga acgataaaga actggctgcc
    23641 gttactgtta ggatcttgtg agcgagccat agataacgca cctcttatat tataaaggtg
    23701 gttgttgatc tctgtttcaa atccttctcc ccaaatgctt tcccctccag tgccatcgcc
    23761 tttaggatcg ccaccttgga tcatgaaatc tttgatgacg cgatggaaag tcagtccgtt
    23821 gtagtaaccg tcttttgcat gtgtcataaa gttttcaacc gcttttggtg cgtgttcggg
    23881 aaataatttg attttgattg tgcctttatt cgtcaccatt tcgaccagat cttcattttc
    23941 ggcgacatct ttcgtcaatt gtggtaattc caatgcgttc aaatcgacgc tactggcagt
    24001 cgtgctttcc gcagtagatg tactttttga actttctgta gacgaactag aagaagtcgt
    24061 gttgtttgca gaacaaccag cagcaaacag caatgctgct gctaaactaa tacttaaaat
    24121 aactttattt ttcttcataa ctgttccttt ccaatttact attttgttat ctttaatgat
    24181 aacaaaatag agggaaaata cctacttaaa ttattggaat ttatcattta ttttaaaaga
    24241 aaaagaaaag gtgtagacca tttactttgt gtttgctata atgtaagtgg atacaaaaac
    24301 gaaaagatgc aggaggaaat ggtaatatgg gaaaattagg aatctcgatt tatccagaac
    24361 ggtcaacatt tgaaaaggac aaagcgtact tggatttagc tcataagtat gggtataaac
    24421 gggtttttac cagtttgctg cagatcaatg atgataaaga aaaagttctt tcagaattta
    24481 aagaagtagt tgattacgcg aatagcctag gcatggaagt aatggttgat atcaatccag
    24541 cgttatttga acagttggag atttcttacg atgacttatc ttttttccat aagatgggtg
    24601 cctacggggt ccgcttagat atcggattca caggagcaga agaagcaaaa atgacgcgca
    24661 atccttttgg tatcaaaatc gagatcaata tgagttcggg cacgaattac gttgacaaca
    24721 tcatgagtta ttcgccaaac acggataatt tattaggttc tcataatttt tatcctcatc
    24781 gatactctgg tttaggatac gaacactttg ttttttgttc agaaaaattc agaaaatata
    24841 atttgaatac gatggcattc gtcaattcgc aatctgctga gtttggccct tggcctacgc
    24901 aagatggctt gtgcacgctg gaagaccacc gagatcttga aattgcgaca caagtgaagc
    24961 atttaatcct tacaggattg attgatgata tttctattgg aaatgcgtac gcaagtgaag
    25021 aagaattgaa agaaatggca gaagcattta acgcggatta tccaacattg aaagtagata
    25081 cggaagaagg tattacagaa aacgaacgaa tctgcttgtt tgataacctt catagttatc
    25141 gaggggatcg ctcagaatat atccttcgtt caacgatgac tcgtgtatac tacaaagata
    25201 aagatttccc gcctcataat acgcgagata tgcatcatgg ggatgtactg attgacaatg
    25261 aagggtacgg tcaatacaaa ggagaaacac aaatcgcctt gaaggatatg aaaaacgacg
    25321 gacgtgtcaa tgttgtagga agaatctccg atgacgaatt attcctcctt gactttttga
    25381 aaccatggtc aagctttaaa ttaattgaaa ataaaaaata gataattagt tattgcagaa
    25441 ataatccgct agaaatagcc agttatttct gcttttttat tttgtttttt tatag
//
LOCUS       DARBFO010000030        24575 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000030 DARBFO010000000
VERSION     DARBFO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.031"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(152..550)
                     /locus_tag="SCK51_001377"
     CDS             complement(152..550)
                     /locus_tag="SCK51_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEG1252013.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="SCK51_001378"
     CDS             complement(547..1320)
                     /locus_tag="SCK51_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEG1252014.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="SCK51_001379"
     CDS             complement(1317..2000)
                     /locus_tag="SCK51_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEG1252015.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="SCK51_001380"
     CDS             complement(2167..3054)
                     /locus_tag="SCK51_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1252016.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="SCK51_001381"
     CDS             complement(3051..3920)
                     /locus_tag="SCK51_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1252017.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="SCK51_001382"
     CDS             complement(4057..5343)
                     /locus_tag="SCK51_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1252018.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="SCK51_001383"
     CDS             5616..6194
                     /locus_tag="SCK51_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG1252019.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="SCK51_001384"
     CDS             6194..7123
                     /locus_tag="SCK51_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1252020.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="SCK51_001385"
     CDS             7120..8856
                     /locus_tag="SCK51_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG1252021.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="SCK51_001386"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="SCK51_001386"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEG1252022.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="SCK51_001387"
     CDS             complement(9752..10261)
                     /locus_tag="SCK51_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEG1252023.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="SCK51_001388"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="SCK51_001388"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEG1252024.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="SCK51_001389"
     CDS             complement(11562..12653)
                     /locus_tag="SCK51_001389"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEG1252025.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="SCK51_001390"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="SCK51_001390"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEG1252026.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="SCK51_001391"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="SCK51_001391"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEG1252027.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="SCK51_001392"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="SCK51_001392"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEG1252028.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="SCK51_001393"
     CDS             complement(16616..17590)
                     /locus_tag="SCK51_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEG1252029.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="SCK51_001394"
     CDS             complement(17571..19454)
                     /locus_tag="SCK51_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEG1252030.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKKHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="SCK51_001395"
     CDS             complement(19472..21697)
                     /locus_tag="SCK51_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEG1252031.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21812..22846
                     /locus_tag="SCK51_001396"
     CDS             21812..22846
                     /locus_tag="SCK51_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1252032.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22913..23926)
                     /locus_tag="SCK51_001397"
     CDS             complement(22913..23926)
                     /locus_tag="SCK51_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1252033.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24004..24192)
                     /locus_tag="SCK51_001398"
     CDS             complement(24004..24192)
                     /locus_tag="SCK51_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEG1252034.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7061 a         5341 c         4396 g         7777 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tgttttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21781 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21841 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21901 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21961 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    22021 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22081 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22141 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22201 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22261 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22321 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22381 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22441 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22501 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22561 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22621 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22681 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22741 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22801 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22861 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22921 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22981 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    23041 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23101 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23161 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23221 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23281 caacaggtcc ccaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23341 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23401 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23461 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23521 ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23581 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23641 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23701 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23761 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23821 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23881 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23941 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    24001 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24061 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24121 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24181 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24241 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24301 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24361 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24421 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24481 tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct
    24541 tattggcaaa tcgccaaaat aatgtagact aacta
//
LOCUS       DARBFO010000031        24260 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000031 DARBFO010000000
VERSION     DARBFO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.032"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(55..789)
                     /locus_tag="SCK51_001399"
     CDS             complement(55..789)
                     /locus_tag="SCK51_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEG1252035.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="SCK51_001400"
     CDS             1116..1526
                     /locus_tag="SCK51_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEG1252036.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="SCK51_001401"
     CDS             1519..2220
                     /locus_tag="SCK51_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEG1252037.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="SCK51_001402"
     CDS             2220..3806
                     /locus_tag="SCK51_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1252038.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="SCK51_001403"
     CDS             3825..4046
                     /locus_tag="SCK51_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEG1252039.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="SCK51_001404"
     CDS             4043..4804
                     /locus_tag="SCK51_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEG1252040.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="SCK51_001405"
     CDS             5184..5693
                     /locus_tag="SCK51_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEG1252041.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="SCK51_001406"
     CDS             complement(5768..6307)
                     /locus_tag="SCK51_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEG1252042.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="SCK51_001407"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="SCK51_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEG1252043.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="SCK51_001408"
     CDS             7320..8171
                     /locus_tag="SCK51_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEG1252044.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="SCK51_001409"
     CDS             8199..8834
                     /locus_tag="SCK51_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEG1252045.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="SCK51_001410"
     CDS             8854..9627
                     /locus_tag="SCK51_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1252046.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="SCK51_001411"
     CDS             10035..10832
                     /locus_tag="SCK51_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEG1252047.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="SCK51_001412"
     CDS             10810..11223
                     /locus_tag="SCK51_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG1252048.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="SCK51_001413"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="SCK51_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEG1252049.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="SCK51_001414"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="SCK51_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEG1252050.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /gene="maf"
                     /locus_tag="SCK51_001415"
     CDS             16000..16560
                     /gene="maf"
                     /locus_tag="SCK51_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEG1252051.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="SCK51_001416"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="SCK51_001416"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEG1252052.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="SCK51_001417"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="SCK51_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEG1252053.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="SCK51_001418"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="SCK51_001418"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEG1252054.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="SCK51_001419"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="SCK51_001419"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEG1252055.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="SCK51_001420"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="SCK51_001420"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEG1252056.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="SCK51_001421"
     CDS             21907..22485
                     /locus_tag="SCK51_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1252057.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="SCK51_001422"
     CDS             22537..23019
                     /locus_tag="SCK51_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEG1252058.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="SCK51_001423"
     CDS             23136..23510
                     /locus_tag="SCK51_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252059.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            23940..>24260
                     /locus_tag="SCK51_001424"
     CDS             23940..>24260
                     /locus_tag="SCK51_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1252060.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT         7952 a         4359 c         5182 g         6767 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt ttgagagtat aaaatatttt gtgtaaatga aaaaatccat acaaaaaagg
    23881 aagtcccttc tgtagaataa agttaacgac aaccaattca cagaaaagag gacttcccta
    23941 tgaatgattt tactacagaa attgtgcaaa ctctagtcac taaaggcgat ttaaatgaat
    24001 tattccgttc gcacttagaa aaagcgataa acacactcct acggactgaa ttaacggctt
    24061 ttttagatta cgaaaaatat gatcgcactg gttttaattc aggtaattcg agaaacggtt
    24121 cttactttcg atcaatcaaa accgaatatg gtgaattaac attggaaata cctagagatc
    24181 gtaatggtga gtttaaacaa caaactttac cagcctacaa aagaacaaac gatacattgg
    24241 aaaccactat tatccattta
//
LOCUS       DARBFO010000032        23971 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000032 DARBFO010000000
VERSION     DARBFO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.033"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            476..1339
                     /locus_tag="SCK51_001425"
     CDS             476..1339
                     /locus_tag="SCK51_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1252061.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="SCK51_001426"
     CDS             complement(1468..3090)
                     /locus_tag="SCK51_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1252062.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="SCK51_001427"
     CDS             3339..3860
                     /locus_tag="SCK51_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEG1252063.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="SCK51_001428"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="SCK51_001428"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEG1252064.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="SCK51_001429"
     CDS             5941..6759
                     /locus_tag="SCK51_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEG1252065.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="SCK51_001430"
     CDS             complement(6862..7059)
                     /locus_tag="SCK51_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252066.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="SCK51_001431"
     CDS             7228..7806
                     /locus_tag="SCK51_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEG1252067.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="SCK51_001432"
     CDS             7901..8038
                     /locus_tag="SCK51_001432"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252068.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="SCK51_001433"
     CDS             complement(8851..9354)
                     /locus_tag="SCK51_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEG1252069.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="SCK51_001434"
     CDS             9713..10738
                     /locus_tag="SCK51_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1252070.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11061..11939
                     /locus_tag="SCK51_001435"
     CDS             11061..11939
                     /locus_tag="SCK51_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HEG1252071.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12415..12531
                     /locus_tag="SCK51_001436"
     CDS             12415..12531
                     /locus_tag="SCK51_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEG1252072.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13459..13797
                     /locus_tag="SCK51_001437"
     CDS             13459..13797
                     /locus_tag="SCK51_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252073.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13891..14121
                     /locus_tag="SCK51_001438"
     CDS             13891..14121
                     /locus_tag="SCK51_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252074.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14521..14892
                     /locus_tag="SCK51_001439"
     CDS             14521..14892
                     /locus_tag="SCK51_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEG1252075.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15166..15858)
                     /locus_tag="SCK51_001440"
     CDS             complement(15166..15858)
                     /locus_tag="SCK51_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1252076.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15861..16646)
                     /locus_tag="SCK51_001441"
     CDS             complement(15861..16646)
                     /locus_tag="SCK51_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1252077.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16883..18283
                     /gene="lacG"
                     /locus_tag="SCK51_001442"
     CDS             16883..18283
                     /gene="lacG"
                     /locus_tag="SCK51_001442"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEG1252078.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18308..18625
                     /locus_tag="SCK51_001443"
     CDS             18308..18625
                     /locus_tag="SCK51_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1252079.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18638..20311
                     /locus_tag="SCK51_001444"
     CDS             18638..20311
                     /locus_tag="SCK51_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEG1252080.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20325..21956
                     /locus_tag="SCK51_001445"
     CDS             20325..21956
                     /locus_tag="SCK51_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEG1252081.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22828..>23971
                     /locus_tag="SCK51_001446"
     CDS             22828..>23971
                     /locus_tag="SCK51_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HEG1252082.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQ"
BASE COUNT         7477 a         4076 c         4843 g         7575 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacgctt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat
    10921 aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata
    10981 gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg
    11041 gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga
    11101 aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag
    11161 agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg
    11221 aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag
    11281 aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa
    11341 tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta
    11401 gatttattca tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga
    11461 tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat
    11521 attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata
    11581 ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc
    11641 gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt
    11701 tactatcacc caatcttact aaatggttga aaaaagagtc aaaaatctgt ctttttgcag
    11761 ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct tggatctttg
    11821 gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa
    11881 aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag
    11941 tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc
    12001 acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa
    12061 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc
    12121 ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac
    12181 tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa
    12241 cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt
    12301 ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt
    12361 tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa
    12421 aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa
    12481 cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga
    12541 aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg
    12601 tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta
    12661 gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa
    12721 ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa
    12781 cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg
    12841 aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt
    12901 catgaaatat ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata
    12961 agcagttacg aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa
    13021 aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg
    13081 ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa
    13141 taataagttt tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag
    13201 taataaagta tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa
    13261 ttaatggtta aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg
    13321 tgatactaga ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa
    13381 tgaaaaagaa gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt
    13441 agccaagtaa cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt
    13501 cgtgctacag aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct
    13561 agtgagccaa ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt
    13621 atggtggtaa ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt
    13681 gaaaaggtga gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc
    13741 attttaacgg attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca
    13801 ctagtgaaat actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa
    13861 tcgatcatca aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt
    13921 tattacgtga atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt
    13981 cgctactcaa ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa
    14041 actgtttttt ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat
    14101 tatgttagta tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt
    14161 tattgttttt tcgatatctt tgctgttaat attactaact ttaatgcagt ataaagagta
    14221 tccagagtat tttattttaa cttttgtata tttagtaagt agctacacac ttctatataa
    14281 attcagaagt tcttctagag tttctaaagt ttgtttctta attagcttat tgattctata
    14341 atatttttga tttatatagg aaattaaaaa tattatagaa tcaacactca gagaacgggt
    14401 tggtttttaa tagatttaat aagtactgat aatgaaattt ctcctggttg ggaaaacgaa
    14461 gttggttacc ctgtagaaga attttttcaa gattactttg cggagtatgc cgataggata
    14521 atgagagatt acgtaacaga taaaactcaa gaagtcatca atcaatccca gtcattaaat
    14581 gaattatctg ataaggcgaa agaagaagca gttgccatta tttcttcagg gattagtgac
    14641 tatattgata tgagtggtga aatcaattat ggcgataaag acaaaattgt acgcatgata
    14701 aaagctttta tcaaaacttt tgatgtgaag gcagatctta agtttaatga tacttaccta
    14761 gtaggaaaat taattaatat ccttgggaca gagaaagata cggaaacgtt tatttcctct
    14821 ttgtctatgt tgtttacaga taagcatttt tcaactcagc ttggtggaga ggaactagtg
    14881 gaaatctttt aaccattacg cccagcatta ataaaagtat atcaaaaaag tactcctgat
    14941 gatttttaag attggtttga gaaataatgt gaaaacacta tttttaatgt taattataga
    15001 tagagatgaa cgcgtcatat ggttaaaaga atgaaaaagt caaagataca ctgctatatt
    15061 tcactaagga tatagcagtg tatctttcat gtcgtttcac tggcagtttt ttataaaagg
    15121 aggtgtgcat ttcagttgct tttctttggt agttagttga atcgtttaag aattaacgga
    15181 tgtggcagac aggtgctctg tccaaaaacc agtaaaatct ttgatggttc catctttatt
    15241 tagctgtgcc tgttcaaata ctgttttgtt tttaccgaat ggatcaattg caataattgt
    15301 tccgatcccc gcacgtttgg cagcaagtaa tcctgagtaa gcatcttcga taacaagaca
    15361 atcagccgga gctaagctca gtttgtctgc cgctttaaga aaaatatccg gttgcggttt
    15421 cccagggaaa gagccgtcgt catagacaat cttgtcccag tcaaaccagc gggctaaatc
    15481 gaaaatatca aaataaaaag caacattctc tttgacagta gccgtggcaa tcgttaatgg
    15541 aatctgttgg tcagtcagct ggtcaagagt cgcttcaagc ccttttgtca actgaagctc
    15601 gttttcattt tgcaagcaaa gcatacgata atagctttct ttttcttctg acaatacggc
    15661 gatctcagag gcggtcaaat gctctgaaat gaaatgtttc aaaatctccg tattcgtccg
    15721 accatgaata ttttttaaaa tatcctcgtc tgataaacca tcttttgtat gatcatgtat
    15781 catatgcagc caagcagctt catgcagatg agagtccaaa aacattgttc cgttgaaatc
    15841 aaatataatc cctttcatag ttacgctcct atagtggtat tttcggttga atcagcaatt
    15901 tgcagatcga catattgtgc atagtgctct tgaacctccc gcgagatctg gcgatccgta
    15961 atgagcagat cgaaatcatg caaattataa aacacataaa aatcatctcg attgaatttt
    16021 ttattatcaa cgagtagata tttcttacgg gaattattta gagcgatctg ctgtgcttct
    16081 ccttcttcgg tactgtaagt agagatcgct tcgttgtgaa cgccatttga actgataaat
    16141 gcttttgtaa actttagtct tcttaaattg tcgttggtga tcggtccgac aaatgcgccg
    16201 gtatttttcc ggaaatcacc gccaatcagt agaatttctg gtccatctgc ttgagcaaga
    16261 atatcaaaaa ctggataatt atttgtaatg atccgaattt tcttgttcag taaatgcgtc
    16321 gccaatagtt cgattgttgt acctggtcct aagaaaatcg tttctccctc gtgaatcaag
    16381 gaggctgctg tggcagcaat ttcttttttt tcttgaatct gcagagtggc tttttcagtg
    16441 tgagaaagtt cttgatctaa agtgtaggaa agactttgag cccctccatg gacacgcagc
    16501 agcttgcccg atttttctaa ttcatctaaa tctctgcgaa cggtcatatc agaaacatct
    16561 aattgattga tgatttcatt aacggtaagg attccttttt tattgaccat ttctacgata
    16621 gtaagcaatc tttctttttt caacattgtc ttcacctcta atttaaggat aatctatcat
    16681 ataaacggac aacagtcaat ctaattcgaa caaaagtaaa catttagggt ggtttttatg
    16741 tttgttacaa tagattttat tgctgttttc tggtttttat gattatttgt gtttgttttt
    16801 gttgacaaat gttttggatg cgtttacaat gcaatcagtt agaaagagcg aaaactgatt
    16861 atagatttga ggagagatgt acatgcttaa attaccagaa gattttattt ttggcggagc
    16921 cacagcagcc tatcaggttg aaggagccac taaggaaggt ggcaaaggcg ctgttgcttg
    16981 ggatgacttt ttagaggaac aagggcgatt cagtcccgat ccggcaagtg atttttatca
    17041 tcaatatgca aaggacattg aattgtgtga acgttttggt gtaaatggtc taagattatc
    17101 cattgcttgg agccgcattt ttcctgatgg agctgggaaa ccaaatccag aaggaatcgc
    17161 tttttatcat cgtgtgttcg aagaatgtaa gaaaagaaac gtaacgccgt ttgtgacgtt
    17221 gcatcatttt gataccccta aacgtttgtt tgaccatgga gattttttga atcgagaaac
    17281 aatagaagca tttgtttcct atgccatttt ttgttttcat gaattcaaag aagtcaaggt
    17341 gtggagtacc tttaatgaaa tctatccagt tgcaaccaat caatatttgt taggtgtttt
    17401 cccaccagga atcaaatatg attttacaaa gatcgtcgcc tgtcttcata atatgatggt
    17461 tgctcatgcc cgtgtagtga actatttcaa agaaaacgaa cttccgggtg aaattggtgt
    17521 agttcactca ttagagacga aatatgcggc aacagacgcg ccggaagata agcatgctgc
    17581 ctttttagac gatgcattgt cgatccgttt cttattagat gctacctatt taggctatta
    17641 ttcaactgaa accttgactg ctcttgatga gatatgtgaa gcaaatcagg ccagctatca
    17701 ttttcctgaa gaagatttcg ttgaattgaa aaaagcatcc acgcgtaatg actacttagg
    17761 gatcaatcac taccaatgcc attttgtcaa agcttacgat ggagaaaatg caatccatca
    17821 taacggtacg ggtgaaaaag ggacctctgt ttataaagtg aagggaatcg gagaacgtat
    17881 ttataaagaa ggaattcctc gaactgattg ggactggctg atttatccag aaggtctata
    17941 tgacttgttg ctgcgaatta aaagcgatta cccacattac aacaaaatct atatcactga
    18001 gaacgggatg gggtataaag atcagtttga agatggcatt attatggatc agccgagaat
    18061 cgactatttg agagtctatt tggaatcctt aagcaaggcg ataacagccg gggtaaatgt
    18121 gaaggggtat tttttgtggt cgcttatgga tcttttctca tggacaaatg ggtataacaa
    18181 aaggtatggc ttgttttatg ttgattttga aacccagaag cgttacccaa aagaatcagc
    18241 ttactggtac aagttggtca gtgaaaccaa aacgattatt taataagtga taatggagga
    18301 agaaaagatg aataaagagg aattacaaat gcttggtttt gagatcgttg catattcagg
    18361 cgacgctcgc agtacattat tgaaattatt gaaagaagta agacaaggaa actttgaaca
    18421 tgttgattcg gcgttgaaag aagccgacga aaatttaacc cttgcacata attcgcaaac
    18481 aaaaatttta gcggaagaag catctgggaa agagatggag atgggattca tttttatcca
    18541 tgggcaggat catttaatga ccactttatt gctgagagat ctgattcaag actttattgt
    18601 actgtacaga cagaatcaag gataggagaa gaacgctatg gatggaatca ttaaacaaat
    18661 cgaaaaaggg aaaccatttt ttgaaaaaat ctcacgtaat agttatttag gagccgttcg
    18721 tgacggattt ttaacagcga tgccagctat tttattttca agcattttca ttttgattgc
    18781 tgcggttcct gagatttttg gttaccaatg gcctgaatct gtctccacat ggctatggaa
    18841 agtctataat tattcgatgg gtgttgtagg gttactcgtt tcagcaacca ctgctcgctg
    18901 cttggctgat tcgatgaatc gtcgcttacc gaaaaacaaa gtaatcaaca ccacctctgt
    18961 gatgctggcc tctgtggtaa gtttcctgtt gttaagcgtc tctccgattg aaggtggatt
    19021 ttcaacggaa tatatgggga caaagggact actggcttcc tttgtcgctg catttttaac
    19081 agttaattta tataagtttt gtgtgctgaa agatattacc attaaaatgc ctaaagaagt
    19141 tcctggaacg atttcgcaaa ccttccgaga tatttttcct ttctcttttt cagtatttgc
    19201 ggccgtttta attgatttag gcattcgaca aatcttccaa atctcatttg cagaagcaat
    19261 cattacggtg ctgcagccgc tatttacagc agctgacggg tacttaggaa tcgctattat
    19321 ctggggagcg atggcgctgt tttggtttgt cggtgttcat ggaccgtcga tcgtggagcc
    19381 ggcaattgcg gcaattatct atgccaatgt ggaaaccaat ctgcaattgt tcaattccgg
    19441 tgaacaagct tctaaagtac tgacggtggg tttagggaat tttgtaggaa ctatgggagg
    19501 aactggggca acattggttg ttccttttat cttcctactc tttgctaagt ctaagcagtt
    19561 gaaggcagtt gggaaagctt cctttattcc agtaagtttc gctgtcaacg aaccattgct
    19621 atttgcggca cccatgattt tgaaccctta tttttttatt ccatttttgt taacacctgt
    19681 tgtaaacgtt tgcctgttca agttttttgt cgatgtattg ggcatgaaca gctttatgta
    19741 cgttttgcct tgggcaactc cagcaccgat cggtttggtt ctaggaacgg gcatgggtat
    19801 cttagctttt gttctagcag cggttctaat cgtcgttgac ttcctgatct atctaccatt
    19861 ttgcaaagca tacgatgctg tcttagtaga gaaagaagca caaaaagcag aggtgatcgc
    19921 aaccgaggaa ggtgcttctg ccgatgtacg tgaggaagta cctgtggcag cgagtcaagt
    19981 tatcagcgat gcaaataaag aaatcaatgt gttggtactt tgtgctggcg cagggacaag
    20041 tgcgatgtta gctaatgctt taaaaaaagg tgcatctgaa tcgaaagcac cgatcagtgc
    20101 aatggcgggt gcttatggtt ctcattacga aatgctgcag gattttgata tggtggtctt
    20161 agcaccgcaa gtcaattctt actatgatga tatcaaagaa gacaccgatc gcttaggaat
    20221 caagctggtt gcgacaaaag gagcagaata tatcggatta acgagagatc ctgaaaaagc
    20281 tgttcagttt attctgaacc aatttcaata aaggaggagc cataatgaaa atgggaattt
    20341 ggttgcgtaa agctgtttta tttgcagctg tgttaggggc tttgggggct gtttcaaaaa
    20401 ctgttgaagc atctgccagc gaatttattc gcggagcaga tatttcgatc ctgtcagata
    20461 tggaaaaatc tggtgcaaaa tattatgaag acgatatttc aaaagatgcc ttagagattt
    20521 taaaaaataa tggtgtcaac tatgttcgac tgcgtttgtg gcaggatcct tataatagta
    20581 atggagaaag ttatggagca gggacaaatg atctggaaac gacgattgac cttgcaaaac
    20641 gagcaaaaaa tctgggactt aaggtgctgc tggattttca ctacagtgat ttttgggttg
    20701 atcctgggaa acagaatctt cctaaagcat ggcaaggtct gacctttgaa gaaatgaata
    20761 cggcattgta cgactataca aagaacgttc tgtcggaaat gaagcagctg gacgtttatc
    20821 ctgatatggt tcagatcggc aatgaactga atagcggaat gctctggcct tatggcaagt
    20881 cttggggaga aggcggggga gaatttgatc gattggctgc ttttttgaag tctggtatcc
    20941 aagctgttcg agacactcaa cctaaaacaa ctccagtaat gctccactta gctgatggtg
    21001 gcgatacagg agcatttact tggtggtttg atgaaatcac gagtcgaggt gtgtcatttg
    21061 atttaattgg agtgtcgtat tatccgtatt ggcacggttc aatgaatgaa cttcaaacca
    21121 atatgaatgc gatcagccaa cggtatggta aagaagtgat cgtagttgaa acagcttatg
    21181 ggcatacaac agcgaatgcc gatactatgc caaatgcttt tggtgaagcc gaagcagctg
    21241 cggggggata tgaaccaaca ccgacaggac aagcagagta tttgctggat cttgccgatc
    21301 ggattcaggc agtaccgaat aatcgcgggg cgggcttctt ctattgggaa cctctttggt
    21361 acaacggaaa tgtgagttgg gcaacacaag caggtatgaa ttatctcagt gttcaatcca
    21421 ctatgggaaa cgaatgggac aaccaagcga tgtttgattt tgctggaaac gccttgccaa
    21481 gcttgcgtgc gtttaaacaa gcaggggcgc aaataaatct agttaaaaat gcaagtttcg
    21541 aaagcgatgg accaacgacc agtccttcca attggcaaac ctggtttcaa caaggaaccg
    21601 ctaagggtac gatcaaaacg gaagcgggca ctttttttgg ggactacaaa ctaacttttt
    21661 ggcaggatca agcatacgag gcatcagctt atcaaaatgt tagtggcata agcaaaggaa
    21721 cttacacatt gagtgcctat gtaatgtctg ggggagagtt tgatactaat cagttatata
    21781 tcaaaaatta tggtggagcg gaactaaata ctgcggttgt taaaacaagt gaatggacaa
    21841 aaattacaat caaagacatt ccaattacaa caggtgtttg tgaaattggg gtttatagca
    21901 aggcttcagg aaatgcttgg acgaacgttg attttgtttc attggtgaaa caatagactg
    21961 cggatcgttt cgattcaaga tcggaggttc ccttcagcca catcgttagc gtagaaggca
    22021 ggaatcgaac ctgcacctcg cctttgccaa aaataaccag aaacgatttc cagtcatttt
    22081 taccaaaacg agtaaagcac cagacttcat ctacactttg tattatgcta aagagagttc
    22141 aacgatttcg taagaagcaa tgaaaaaatt gcaaaaagtt gtgcagataa cataaagttt
    22201 gtttaagaaa gggtaacctt agaattccgt cgtgaatatg atttgagcac ttagatggat
    22261 agaaaaaagt tctcggaaaa cagttcgttt ttcgagaact tctttttatg ataagaatga
    22321 aacaaattaa cttatattat agtctctttt ggtagggaac catcttattt ttaatgaaaa
    22381 tcaagcattt tagctattga agaatgttga tttataggga ttccccctcc atattatgtt
    22441 ggctctttat tcccactctg ttttcaacgt aaagtgtata aaacagaatg cgatactatc
    22501 aagggagtta aaacaatata cagaacagta aaacactata aataaaaata aaagtgcaat
    22561 aaaagtgcaa tttttgcacc taatcatact ctgtttatat atgaaaacta gattctgaat
    22621 ccaatatttg gttaacgaga ggcgttcgaa tttgaacgtc ttttatatat tactttcaaa
    22681 taaatatgag gtagaatact atctattttc tttttaaccc aattgttata atggagagtg
    22741 taaaatattt tgtgtaaata gaaaaaagga agtcccttct gtagaataga gttaccacaa
    22801 cacattcaca gaagagagga cttccctatg aacgatttta ctacagaaat tctaaagact
    22861 ctagcgaaca aaggcgattt gaatgaatta ttccgtgtcc atttggagaa agcagtcaat
    22921 acgcttctca aaacggagtt aacggctttc ctagattacg aaaagtatga tcgcattggt
    22981 tttaacacgg gtaattctcg taacggctcc tatgaccgta cggtcaagac cgagtatggg
    23041 gaacttcatc tccagattcc gcgcgaccgc aatggtgagt tcaagcaaca gactgttcct
    23101 gcttatagac ggacgaatga cacgttagag gagaccgtca ttcacctctt ccgaaaaggt
    23161 attaccatgt cggaaatcgc agacttgatt gagaaaatgt atgggcatta ctacacgccc
    23221 caaaccatgt ccaatataac aaaatcattt acagaagagg taacggcgtt taatgggcgg
    23281 gagcttcatg accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg
    23341 aaaaccgtcg ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag
    23401 gaagtattga gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta
    23461 ttggaccttc aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag
    23521 gggatggtag gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta
    23581 cacgtttccc gtaatatcag tcataaagtg cgtgtcgatg atcgtaagga agtctgtgat
    23641 gattttaaaa tggtgtatca agcctcatct aaagaggtgg cgttggaagc acgtggtact
    23701 tttgcggaaa aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat
    23761 cacttgctca ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac
    23821 ttgattgaat cctttaataa acaaatcaag aaatacagcc accgcaagga acagttccaa
    23881 aatgaagagt cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc
    23941 ctaggtcgca gtcataaagg tttccaacag g
//
LOCUS       DARBFO010000033        23417 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000033 DARBFO010000000
VERSION     DARBFO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.034"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            39..1700
                     /locus_tag="SCK51_001447"
     CDS             39..1700
                     /locus_tag="SCK51_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1252083.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1770..2117)
                     /locus_tag="SCK51_001448"
     CDS             complement(1770..2117)
                     /locus_tag="SCK51_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEG1252084.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2201..3142
                     /locus_tag="SCK51_001449"
     CDS             2201..3142
                     /locus_tag="SCK51_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1252085.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3143..4186
                     /locus_tag="SCK51_001450"
     CDS             3143..4186
                     /locus_tag="SCK51_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1252086.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4192..5262
                     /locus_tag="SCK51_001451"
     CDS             4192..5262
                     /locus_tag="SCK51_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1252087.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5252..6205
                     /locus_tag="SCK51_001452"
     CDS             5252..6205
                     /locus_tag="SCK51_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1252088.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6217..7227
                     /locus_tag="SCK51_001453"
     CDS             6217..7227
                     /locus_tag="SCK51_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1252089.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7512..8333)
                     /locus_tag="SCK51_001454"
     CDS             complement(7512..8333)
                     /locus_tag="SCK51_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG1252090.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8430..8708)
                     /locus_tag="SCK51_001455"
     CDS             complement(8430..8708)
                     /locus_tag="SCK51_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252091.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8976..10985
                     /gene="metG"
                     /locus_tag="SCK51_001456"
     CDS             8976..10985
                     /gene="metG"
                     /locus_tag="SCK51_001456"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEG1252092.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11464..12051)
                     /locus_tag="SCK51_001457"
     CDS             complement(11464..12051)
                     /locus_tag="SCK51_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252093.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12226..13548
                     /locus_tag="SCK51_001458"
     CDS             12226..13548
                     /locus_tag="SCK51_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1252094.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13703..14479
                     /locus_tag="SCK51_001459"
     CDS             13703..14479
                     /locus_tag="SCK51_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEG1252095.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14472..15041
                     /gene="rnmV"
                     /locus_tag="SCK51_001460"
     CDS             14472..15041
                     /gene="rnmV"
                     /locus_tag="SCK51_001460"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEG1252096.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15034..15918
                     /gene="rsmA"
                     /locus_tag="SCK51_001461"
     CDS             15034..15918
                     /gene="rsmA"
                     /locus_tag="SCK51_001461"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEG1252097.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16369..16998
                     /locus_tag="SCK51_001462"
     CDS             16369..16998
                     /locus_tag="SCK51_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252098.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17122..18231)
                     /gene="ugpC"
                     /locus_tag="SCK51_001463"
     CDS             complement(17122..18231)
                     /gene="ugpC"
                     /locus_tag="SCK51_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEG1252099.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18440..18862
                     /gene="mgsA"
                     /locus_tag="SCK51_001464"
     CDS             18440..18862
                     /gene="mgsA"
                     /locus_tag="SCK51_001464"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEG1252100.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19185..19721
                     /locus_tag="SCK51_001465"
     CDS             19185..19721
                     /locus_tag="SCK51_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEG1252101.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19761..21524)
                     /locus_tag="SCK51_001466"
     CDS             complement(19761..21524)
                     /locus_tag="SCK51_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1252102.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21527..23242)
                     /locus_tag="SCK51_001467"
     CDS             complement(21527..23242)
                     /locus_tag="SCK51_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1252103.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7542 a         4288 c         4771 g         6816 t
ORIGIN      
        1 ttaattattc tgaaaattcc aataaagggg tacgtaaaat gaaaaagaaa tttacatttg
       61 gtttggttgc ggtatgcggc cttgtacttg ctggatgtta tggcggcagt tcagccacaa
      121 gcagtagttc agcaaaaagc ggttcagcag atggaggagg cgtatttaac ttagtcgtgc
      181 ctcaagaaat gcctacagct gacttatcag ttgcaacaga tacaatcagt tttacagcat
      241 tgaataacgt ttatgaagga atttaccgtc ttgatgaaga cagcaaacca cagccggcag
      301 gagctagcga gttggcagaa gtcagtgaag acggattaac ttacaaaatc aaattacgag
      361 aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc ggattacgtt tatggctggc
      421 aacgaacagt agacccagct acagcttctg aatatgctta cctgtatgcg ccagttgaaa
      481 atgcagaaga cattacagct ggaaagaaag ataaatcaga actagggatc aaagcagttg
      541 gtgactatga attagaaatc aaattgacga aacaaacacc atatttccaa tatctgcttg
      601 ctttcccatc atttttccca caaaaacaat ccgtagtgga agaaaatggg gatgcctatg
      661 catctgcaag tgataaagct gtttataatg gaccattcac attagaagat tttgatggac
      721 caggaacaga tacagaatgg acttataaga aaaatgatca ttactgggac aaagatacag
      781 taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc ttctactgca ttgaacttat
      841 tcaaagatgg ccaagcagat gacgtgatct tgtctggtga gttagcacag caaaacgcaa
      901 acgatccagc ttatacttct gttaaagaag cgcggacaag ctatatcgaa ttcaatcaac
      961 gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa agctatttct tattctatcg
     1021 atcgtgaagc tttagtaaaa caagtactag gtgacggatc agttgtctct acaggattga
     1081 tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga ctttgcaaaa gaagctggaa
     1141 aattagtttc ttatgacaaa gataaagcaa aagaatattg ggaaaaagcg aaaaaagaat
     1201 tgggcgttga ttctttagaa tttgatttga tggcttctga tgatgattca tcgaaaaaag
     1261 taatcgaata tgttcaaaac tcaatccaag aaaacttgga tggcgtaaca gtcaaaccaa
     1321 cacctgttcc attctctgta cgtcttgacc gctcaacttc gggtgatttt gacgcagtac
     1381 taggcggatg ggctgctgac tatgcagacc caagcagctt tactgatttg tttgtcacag
     1441 gtaattcata caaccgtggt cgctggagca atgcagaata tgacgaagca gttaaagcag
     1501 cttcatcaac agacgctggc gatgaacaag cccgttggga tgatttccaa aaagcaatca
     1561 agatcatctc tgatgatatg ggtgtcgttc cggtgtacca aaaagcagaa ggtcacctag
     1621 tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg cgcttcatgg gattacaaat
     1681 ggacatacgt tgaagaataa tcgttaatac cgtgagcatg agaaaaaacg aggctgcggc
     1741 aaatgtcgca acctcgttct catattcact tatcctaaca aagaaaaaag catgaaaata
     1801 gcagaaagag caattagtat tccggcaatg atcagccaaa agctgtacat accgtagctg
     1861 gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt tccttgttcg tgcaagatgg
     1921 accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga cccataaaaa accgccgata
     1981 atcagaaacg gcaatgacca taagaataga tcattagata attgaatgag tgtaaaggaa
     2041 tggttgatga gattttttat taaaataacc agaatcagta gaaaagctac tgcgtagggg
     2101 atagattttt ttttcatttg attgctcctt tatttttggt tcattatcta ataatgtagc
     2161 gaaacatctg tcagaagtca aaagctaggg ggaaacatta gtgaatagtt atatcaaata
     2221 tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg ttaattgcaa caatcacctt
     2281 tttcttgatg caattgctgc cgggcacgcc atatacgaat caggaacgtt tgagtcctga
     2341 aacaatcgag atgctaaata aacaagtcgg tttggataaa ccagttatcg tacaatatgg
     2401 gatttatctt tctaatttgc ttcaaggaga ttttggaatt tccttccaat ttaaaaacca
     2461 gccagtcgca catttgttag ctggaagaat cggaccatcc ttgcagttag ggttacaggc
     2521 aattatcttt ggaacagtcc tagggacgat tttaggaacg atctctgcca tgaagcaaaa
     2581 tacatgggca gatacatctt ctacactcgt agcgatatta ggtcgttcca ttccaaactt
     2641 tgtatttgct gttttattac aatatatctt tgcaatcaaa ttacaagttt tgccaatcgc
     2701 aaaatgggat ggctttgtgt acacaatctt accaacaatt gcgttggcca tgtcgccgct
     2761 tgctgactct gctcggttta tccgtacgga aatggtggaa gtactgcata gtgattatgt
     2821 ggaattagca aaagcaaaag gattgagccg ttgggaaatc gcctttaaac atggtcttcg
     2881 aaatagtttg attccattga tgactttatt aggaccgtta gctgtagcat tgatgaccgg
     2941 atcactggtg gtagaaaata tctttgctat tcctggtatc ggggaacaat tcgttaagtc
     3001 aatcacaaca aatgattatc ctacgatcat ggctgtaacg atcctttact catttatgct
     3061 gatttttgtt attttgatcg tggatctatt gtacggtcta gttgatccaa gaattcgtgt
     3121 ttcagaaggg agccgaggat aaatggaaat gataccaaag cgttatgaaa cagttgcaga
     3181 tatccctgcg gatgaatttc ttccattaca aaaaaatacc gaagaagaac gtgaacagat
     3241 tactgcacct tctttgaatt ttatccaaga ttcatggcgc cgtttaaaga aaaacaaagc
     3301 tgccgttatt tcaatggttt tactattggt catcattttg atctctgtga tcacaatctt
     3361 tgtttctcct catgatccaa ctgcccaaaa cgtggcttat atcaatttgc ctcctcgtat
     3421 tccgggaatc aatatcgatg gattgaatgg aaaagcaatg gttggtggtg aacttgttga
     3481 taaatatgct caagcaaacg tcccttcaaa cgtaaatttc tttttaggaa cagacggttt
     3541 gggacgtgat gtattaagcc gcttatttat gggaatgaga atctcgttgc tgatcgcatt
     3601 tattgctgca ttactagatg taacgattgg tgtagcttac gggttggttt ctggtttgtt
     3661 aggcggacgt gtcgataacg tgatgcaacg ttttttggaa atcttatcag gaattccaaa
     3721 cctcgttgtc atgattttga tgttggtcgt ttttgaacca ggaatttttt caatcgtggc
     3781 tgctatggcc atcacaaact ggataccaat ggcacgtatc gttcgtgcac aaacaatgaa
     3841 attaaaagat caagaatatg ttttagctgg attgacatta ggtgaatcaa gaatgaaaat
     3901 tgcattcaaa catattttgc cgaatatctc tagtgtcatt attattcaaa tgatgtttag
     3961 tattccatct gctattttct tcgaagcctt tttaagcttt atcggtttag gtcttacgcc
     4021 tccgtcagct tcattaggaa cgatgttgag cgaagggtac aagacattcc tgtatctgcc
     4081 ttatctatta tggattccgg cagtaacttt atcggttatc atgattggct ttaacttact
     4141 tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa gagtgaagac gatgacgaaa
     4201 attttagaag tgaaagactt agaaatctct tttgatacgt atgctggaaa ggtacgagcg
     4261 attcgcggtg tcaattttca tttgaataaa ggagaaacat tggcgatcgt tggggaatct
     4321 ggaagtggaa aatcagtgac gaccagaagc atcatgcgct tgctgtcaag caacgccaat
     4381 atagatgctg gtcaaatcct ctttaaaggt aacgatatcg ttggaaaatc agaaagagag
     4441 atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc aagatccaat gacctcttta
     4501 gatccgacga tgcccattgg gaagcaagtc gctgagtccc ttagaaagca taataaagtt
     4561 tcaaaaaaag agggacaaaa agctgcatta gaattgctta aattagttgg aatacccgaa
     4621 gctgagaaac gtatcaacag ttatccgcat cagttctcag gaggtcagcg tcaacggatc
     4681 gtgatcgcga ttgcactggt atgttatccg gaaatcctga ttgctgatga acctacaaca
     4741 gcgcttgatg tgactattca agcacagatt ttggagctgc tgaaagaaat tcagacaaaa
     4801 atcgatacat caattatctt tatcactcat gaccttggtg tagtagcgaa tgtcgctgat
     4861 cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg gaacatcgga agaaattttc
     4921 tacaacccgc aacatcccta tacttgggga ctgcttggtt caatgccgac attagatagt
     4981 gcgaacgatc gattgtatgc tattcctggt tctccaccgg atttattgaa cccgccaaaa
     5041 ggagacgcat tctatccaag aaatgaattc gcgatgaaaa tcgatgcaga acaagaacca
     5101 ccattttttg aactatcaaa aacacacaag gcagcaactt ggctacttgc tccgcaggca
     5161 cccaaagtga caccgccaga agaaatccaa cgtcgctggg ctatctttaa agagaaacaa
     5221 aaacatactt acggtcaagg aggaattgct gatgccaaat aaactacttg aagttaaagg
     5281 attgaaacag tatttcaatg taggaaaaaa gaatgaagta catgctgtaa atgacatcag
     5341 ctttcacatt tatgaaggag aaacattcgg tttagttgga gaatctggaa gcggaaaatc
     5401 cactactggc cgtacgatca ttcgcttgaa tgaacctact gatggtgaga tattattcga
     5461 cggtcaagat ataacgaaga tcaaagataa aaaaggtttg acgaaattcc ggcatgatgt
     5521 tcagatgatc tttcaagatc catacgcttc gttgaatcct cgtatgaaag tcagagatat
     5581 cattgccgaa ggaattgatg ttaatggact agctaaatca tctgaagagc gtgcaaaaaa
     5641 agtcgatgat ctattgagaa ctgttggatt gaatccaagc cacggtactc gttatcctca
     5701 tgaattttca ggtgggcaac gccagcgtat tggtattgca cgagcattgg cagtaaaacc
     5761 gcgttttatc atttgtgatg aaccaatttc tgcactagat gtgtcgatcc aagcccaagt
     5821 ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg acctatctat ttattgcaca
     5881 tgatttgtcg atggttaaac atattagtga ccgaatcggt gtaatgcata acggcttgtt
     5941 attagagatg ggaacaagtg atgaaatcta taatcacggt gttcatcctt atacagaaag
     6001 tttgttatct gctattcctc taccagatcc agaccatgag agaaaacgca gaagaatcaa
     6061 atatcaacct gaaccagatg acggacaagt acgtcaactt cgtgaaatcg caccagaaca
     6121 ttttgtctat gcaactgagc aggaagtccc atattacgca aaaaaactaa aacgacaaaa
     6181 ggaatctctt ctggttgcta actaggaggg acatagatgc taaaagcttt taaattccgt
     6241 atgtatccga cagaagaaca aaaacagcaa ttgattcgta cttttggatg tgctcgattt
     6301 acctataacc atttgctgaa aaagcggcag aaaagttggc aacaaacagg agtagccaat
     6361 ttttcgctca cacccgctac attaaaaaaa gaatacccat tcttaaaaga agtagacagt
     6421 cttgctttag caaatgcaca gcttaatttg gatcgggctt ttcgcaatta tttcaaggga
     6481 cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt ggcaatctta tacgaccaac
     6541 aaccaaaccc gtacgatcta tctggaaaat ggctacttaa agttaccaaa acaaaaagaa
     6601 ctaatcaaga taaatcagca tcggccagtt gaaggatcga ttcgctccgc tacgatttct
     6661 gctcgatata atgaagaatt ttatgtcgca ctactttgtg atgtttcgcc agtaaaaaaa
     6721 gaatctttag ctaaatggat cgggatcgcc tatcatccga agacattgat ccaaacatct
     6781 cgacctctag aagtaacttt accaaaattt caccagacag aagaaaagct gcaacatgca
     6841 cagcggaaat tgaatgtgaa agtccgctcc gctcaccata gaaagatcag gttagatcag
     6901 gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc tttatctgaa acaaaagaac
     6961 cagcgagaag attatcttga acaattatct gggaaattgg tcaaacaata tgactactta
     7021 tttgtagaat cttttccaaa agaagaggca catgctgatt tttctattca cgactggcat
     7081 aaattaataa caaaattgcg ttataagtcc caatggtata ataaaaaatt tcttttgatc
     7141 aataccgacg gagcagaaga aagcaattcc gtaagaaaga gccaagtagt agaaaaaatg
     7201 ggcagacatt cggttatcaa gggataaaaa atgaatgaaa ctgataggag cgttagggtt
     7261 agccttgtta ataaaaagtc ttaatgactt ttgtttctag gaatttttct tttgaagaaa
     7321 agctcactaa ttatgacaaa ccaataaaac aatagcagaa aaagttcgta cgagcttttt
     7381 ctgctattgt taatttttta ttcagaaagt ttacgaatac atcaaaattc tgaaacaaaa
     7441 aacgtaactc acaaagagtt gtttttgcgt tttttcttga atgattaggt ctgaatcttt
     7501 tgctcacaac cttattcatg gtgccccggc ttgagctgat agatttttga aggacggccg
     7561 attcctacag gacgttcacc gatctcatcg aaatattgga gcatagcttt cttaaaattg
     7621 gaatgatcga tcgttttata atcgatccct aagaatttag caaatacttt tcgagcttcc
     7681 gtaatcgtga aatctttacc taaaacttgt agaacttgcg gctcgtgttc cattttattg
     7741 actacacggt tgaatgcttt caagatgatt tgactatggt caaaagccaa agtatctttg
     7801 ccagaagact ctccagtgac taaatcaagc gagatagcca cttctccgct agataaatgg
     7861 atcttgttac tatgacgttc tagtgtgaac cagcgtactt ccttggcatc atctccggcg
     7921 attagcggtt cttctccaat aaaggctaag tagctgactg tgacgaccca gcctcgcgga
     7981 tctcgattag gcgtactgaa tgtatgcagt tgctcaatat tttcttttga aataaccaca
     8041 ccagtttctt cctttgtttc tcttaagacg ctttctccag ttgactcatc cttttggaca
     8101 aagccaccag gcaatgccca tgaatttcga taaggatgcc cttttctttg gatcaacaaa
     8161 actttcagct gatcttcttc tttgttatag cacatcagta cgatatcgac agtcaaagag
     8221 ggcttgtcat attctggacg ttcctgtttt tgataccagt taagaaattc ttcttcactt
     8281 gctttatgtt cataatataa tttttcttct tctttagaag caaaacggtt catctgacca
     8341 cctccattga cagtaaaagt actttttacc ttattgtagc agactctgaa aaaaaaccaa
     8401 ggatttactt gaaaaatcta ggttatcatt taaatatgaa aaataaatgg aagtatgaga
     8461 ttgaataaaa agaaaagcag ataaaacggc aatactttat accgctcttc acgaatggca
     8521 aagaagccac aaataacgat atatacgtca atgataaaac gcaaaatcca agctgctgtc
     8581 ataaaactgt tcaaatgccc atggaggtta aaataaccat acatacgaat ataaaaatag
     8641 ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa gtgtaaaaaa aagatgtttc
     8701 ttctgcatga ttgttccagt cccttactaa gatagatagt ctttattata ccacgtgtga
     8761 ggggagaaat aaaaagccgt tttttagtag atatgaaaca aaacaaaaac ccgcatttat
     8821 tcaacctatt catcctttta aaggatagat tgaacgtaga tagaagtcgt ttatccagtc
     8881 tttttcattt tgaaaaaaag ctaacagcat gcttttttta tggtatacta gcaaggaaac
     8941 gaattcgcat catgcgataa aggaggaaga gccccgtggc agaaaaggaa actttttata
     9001 tcactacgcc aatctattat ccaagcggca aacttcatat cggcaactcg tatactacga
     9061 ttgcatgtga cgcaattgct cgttataaac ggttgatggg ctttgatgtg ttttatctca
     9121 ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc agaagaacta ggtgtaaaac
     9181 cgcaagaata tgtagataaa atggcggctg atgtcaagaa attatggaaa acgcttgata
     9241 tcagttatga taaattcatt cgcacgacag atgattacca taaagcagct gttcaaaaaa
     9301 tatttgatcg tttattagaa cagggagata tttacttagg tgaatatgaa ggctggtact
     9361 ctgtttctga cgaagaattc tttacagaaa cacaacttgc agaagtgtac aaagatgacg
     9421 aaggtaacgt gatcggtggt aaagcgccaa gtggacacga agtagaattg gtaaaagaag
     9481 aatcatactt cttccgcatg agcaaatacg cagaccgtct attggaatac tatgaagaac
     9541 atcccgaatt catccagcca gaatctcgta aaaacgaaat gatcaacaac tttatcaaac
     9601 caggattaga ggatcttgca gtttcacgaa caacttttac ttggggagta ccagtcaaga
     9661 gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt atccaattat attactgcac
     9721 taggatatgg ctccgaagat gaaagtctat tcgaaaaata ctggcctgca gatgttcaga
     9781 tggtcggtaa agaaatcgtt cgctttcata cgatctattg gccaatcatg ctgatggccc
     9841 ttgatttgcc tctacctaaa aaagtatttg ggcatggctg gctattgatg aaagacggaa
     9901 aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat gcttgtggaa cgttatggtt
     9961 tagatgcact ccgttattat ttgcttcgtg ctgttccgtt tgggtcagat ggtgtcttta
    10021 cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc aaatgatcta gggaacttat
    10081 tgaatcgaac gattgctatg atcaacaaat attgtgatgg actcgttcct gactatgcct
    10141 cacttgtcac tccatttgat agtgaactgt ctacgacggc tgctaacgtt gtcggacgtt
    10201 accatgatgc aatggaaaaa atggaattca atacagcatt ggctgaaatc tgggtgctga
    10261 tttctcgtgc aaacaaatac attgatgaaa cagaaccatg ggtattagca aaagacgaag
    10321 aaaagaaagc tgaattgaat agtgtcatga tccatttagc tgaatctttg cgtatcgcag
    10381 cgatattact tcagccaatc atgacagaaa cgccagagaa gatcttcaat caactaggat
    10441 tggactctga aacaatgaat cttgaaggat tacactttgg cgaatttcct tcaggaacaa
    10501 aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga tatggaagaa gaagttgctt
    10561 ttattcaaga aaaaatgtct gaaggtactc aaacaaacga agatacagtc aaatgggatc
    10621 cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat caaatttgat gtatttgaaa
    10681 aagtagaact aaaagtagca gaagtcatca actgtcaaaa agtagaaggg gcagataagt
    10741 tgctgcaatt ccgactagat gctggagaca gccaagaccg tcaaatcttg tcaggaatcg
    10801 ctgaatttta tcctgatcca agcgaattga tcgggaaaaa agtcgtcatt gtagcaaatc
    10861 tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat gatcctttct gcagaagcac
    10921 cagatggctc tttacaagta atcgaagcac caaaatccat gccaaatggc tcagaaatcg
    10981 catagaaatt caaagggggt atgacactag ttgtgtcata cccccttttt ccgaaatttg
    11041 agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc aatcatataa tacagaaaca
    11101 gagaactatc caaaataaat cctagaaaaa gaagtacgag cttcgctgat ttttgttaat
    11161 ttagaataat actaatagga atcgtaaaaa cagaaaaaaa gcataatgat aaagggtcgg
    11221 gcagatttta attgattcat ctggtactct tttttattgt ccttacattc ataaagtttg
    11281 aatgcaatga ctttattaca tctatattta tatctaacag tagcgttgat gtgttaatta
    11341 taagttctat cactttatgt tcaattagtt tgtgctctta tatgtgtgat tgaaaatata
    11401 gataaaaaaa caatcatcgg ctttgacttc gatgattgtt ttctttattt tgtaaaaatc
    11461 ctatcacttg attttatggt aaccttttat ttttgtcttc aaacggcttg gtaagagagc
    11521 agtaagcagt gcaaatggga tcgaaaagac ataccagaaa atcgtacgtc cgataccata
    11581 atgttgatag agctttgtca acatcacgtg taacaagatc aaccctaaaa gcaaggagcc
    11641 agctataaac caatgattat cccaatgcat gacttcacta agtgccatca ctccaaaagg
    11701 atagaaataa aaatagatcc cgactaaggt catccgccag ttctttgtaa atgttccgat
    11761 attcaataaa aagaaaatcg ctgaaagcaa aataccgata ggcggaaaaa gcccaaccaa
    11821 aaaggaatac aacatccccc acaagatcat cacaggacct ttgatcaatg caacgattgc
    11881 gatgaacaaa gtcagcaata ttagtttttg tgataacgtt gcaacagtca ttaatccaaa
    11941 taaaattaat gtgaaccaat gccaatggga ctgctgttgt tctttacggg aaaaatcgac
    12001 ccggttggat aaataatctg ctaatttcgg ataagaacgc ttaaaatcca taaactgttc
    12061 ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa aaaagaaata catccgtctt
    12121 aagagggagt ttttcactga aagtggagaa aaaagaatgg taaattaaaa tattctcagt
    12181 tatactgaaa attataccaa atttcaaaga agaaagagga tgggaatgaa aggcaaacta
    12241 aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta tgggaaccgt gatcggtgca
    12301 ggcgtcttct tcaaaactgc tgctgttact gctagtactc agtctacaag tttgacattg
    12361 ctagcttggc ttttgggtgg tttcttaacg atctgtgctg gattgacagt ggctgaatta
    12421 gctacagcaa tcccggaaac tggtggagct gtaaaatata ttgaagctgc gtatggaaaa
    12481 ttgcccagct ttttattagg atgggcgcaa agcttgattt actttcctgc taatattgct
    12541 gcattatcga ttatctttgc gacacaattg acgaacttat tacaattatc taccgattat
    12601 cttttgttga ttgcagtgat caccgctgtt tctgtgacag ggttgaattt gttagggaca
    12661 aaagtcggtg cttctgtaca atcagtgacg ttgattgtta aattaatacc aattgctgtg
    12721 attgtcatat ggggattatt gaccccagga caaggaactg tccagttatt tccaatcgaa
    12781 gctggaaaag atgttacttt tgtcgaagga ttgagcagtg cgttgttagc aacgttgttt
    12841 gcttatgacg gctggttagg tgtcggtgca atggcagggg aaatgaaacg gccggaaaaa
    12901 gatttgccta aagcaattat tcttggtttg agttttgtaa cagtcgttta tctattgatc
    12961 aattttgttt ttttgaaaac tttgccgatt gatcatcttg ccggtaattt gaatgcagct
    13021 tctgaagcat cagacgtcat ttttggagga atcggtggaa agctggtcac tattgggatc
    13081 ttgatctcgg tatacggtgc attgaacggt tatacactaa ctgggatccg cgtgccttat
    13141 gcgatggctt tagaagacga gctacctttc agtaaacagc tgacaaagct ttcaaagaag
    13201 tttacggttc cttatgttcc tgcggttttc cagttagcag tagcttgtat tatgatgagc
    13261 cttggttctt tcgacttttt aacagacatg ttgatttttg tgatgtggct attcagttta
    13321 ctgattttta tcggtgtctt tattttaaga aaaaaagcgc cggaattgcc acgtccatat
    13381 aaagtaccac tttatccaat cgttccgatt attgcgattc tcggtgctat tttcatttta
    13441 ggtatgacga tgatgacgca aacgaaatta gcattgatcg gtataggtgt taccttgata
    13501 ggtatcccag tctattatca aaaaaagaaa aaacgctcag aagagtaaaa tatttcaaca
    13561 acagacagaa aaagtacgaa ctaggtcctc tagatcttcg tgctttttct atttcaataa
    13621 aacaagttct tctattttga tttttgctac ttctgcaagt agtgcttatg tgctaaagta
    13681 agaatgaaaa ggagagataa acatgatatt tgattctcat acccatttaa atgctgaaca
    13741 gttcaatgaa gatattccag aaacgattca gcgagcacaa gaaatggggg taacgaaaat
    13801 ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct ttgcaattaa gccatgacta
    13861 tccaaatata tacagtatta tcggctggca tccgacagag gctggcagtt atacgaaaga
    13921 aatcgaaaat aaattacaag aacaattgac tttaccaaag gttgtagcat taggggaaat
    13981 tggcttggat tatcattgga tggaagaccc gaaagaagtc caagataaag tatttcgcag
    14041 acagattgct atcgcaaaag aaatgaacct accaatcagt attcatacta gagaagcact
    14101 tgaagacacg tatcaaatcc taaaagaaga agatgttcga gatatcggtg gaatcatgca
    14161 tagtttcagt ggtgattatg aatgggcaaa acgatttctt gacttaggga tgcatatttc
    14221 atttagcggt gtcgtcactt tcaaaaaagc attagacgtc caagaggctg cagcaaatgt
    14281 accaatcgaa cgattacttg tggaaacaga tgccccttat ctggcaccag ttccttttag
    14341 aggaaagcga aatgaaccag gctatactcg ttatacagta gaaaagattg ctgaacttcg
    14401 gaatcttccg acagaagaag tggctgccca aacatggaca aatgcgcacc gattatttgg
    14461 tctaagcgaa catggataag atgaagatcc aagagattat agtcgttgaa ggaaaagacg
    14521 atacaagaag gctgcgggaa gtagttgatg cagatacgat agaaacaata gggtctgcaa
    14581 tcaatgaaga gatcctgatg caaattgaac atgcccaaga gactcgtgga gtgatcgttt
    14641 ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat cattatggaa gctgttcctc
    14701 aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc aaagaaaaag ggaagccttg
    14761 gtgtcgagca tgcaagtgat gctgctattt tagaagcatt gaaaaaggtc gtgacgccag
    14821 acatttccca aacggctgtt tctgaaatca ctagacaaga ccttttagac tatggtttaa
    14881 tagctggggc acaagcaaaa gaaaaacgag aaaaactagg tgacgagttg cgcatcggtt
    14941 acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt tcgtattacg ccgaaagaac
    15001 tcgcccaagc aatgaaaata gtggaggaac aacatgagtg aatatcgtga aattgcgaca
    15061 ccctctagaa caaaagagat tttattgaaa catggttttt catttaaaaa aagtttagga
    15121 caaaattttc tgactgaacc aaatatttta aggaaaatcg tagaaactgc gcaaatagat
    15181 gaacatacga acgtgatcga agttggacca ggaatcggtg cattgacgga acaattagcc
    15241 aagcatgcaa aacaagtcgt agcatttgag atcgatgatc gtttgatccc tgtattggct
    15301 gatacgatgc aaccctatga caatgtgact attgtacatc aagatatttt aaagaccgat
    15361 ttgtcaacag ctgttagaga aacatttcat gaggagcttc cgttaaaagt cgtagcgaat
    15421 ttaccttact atattacgac accgatcatg atgcattttc ttgaatcaga tttggttgtg
    15481 gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc gtatctctgc tgaaccagga
    15541 acgaaagctt atggttcatt atcgattgct gttcaatatt atatggaagc cagtttagca
    15601 ttcatcgtac caaaaacagt atttgtccct cagccaaatg tagattcagc gattttgaaa
    15661 ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg aaaaagcatt tttccgattg
    15721 acaaaagcgg catttcagca aagaagaaaa acgctttgga ataacttgca gcattcgtat
    15781 ggcaaagatg accagacaaa agcatggtta gcaaaaagct tggaaacagc tggaatcgat
    15841 ccaaaacgtc gtggggagac tttgtcatta caagaattcg ccgcattaag caatgcgatg
    15901 tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt tgtgaaaaag ctgtcctgca
    15961 tcaagtaata agaacagcca aacaaaaata gttcctcatg tttttcgttt gtttgggctt
    16021 gttaccgcag atgcaagctt ttgaacaccg tttaagaaag aggttgtggg cctgccgttt
    16081 aactctaagt cacaaggaac aatttcaaaa aacagcttct catgtttttg agttagttgg
    16141 tcttattact tgatgcaagc ttttgaccac tattatgccg aacaaggaga tgtgacacaa
    16201 attttgtcac atctcctatt tttgtttctt gatcaaaaaa ttcttatact tgatactcac
    16261 actcattttg tatggattta gaaaagaata tgtgtcttga cagttattag taagtgagtt
    16321 atactaagtc gacatttatg tgtcaagaca tattaggaga gagaaaaaat gagaaaaaat
    16381 tcagttcgaa aactaacgat ttctgcttta ttgattggga tgggggtcat tatcccaatg
    16441 gtcatgccta aaatcatgat tccaccagct tcctttactt tggcgtctca tgtgccatta
    16501 tttattgcga tgttttttac accaggagtt gcagtggctg ttgcgctagg tacgacattt
    16561 ggtttctttt taacaacgcc ggttatcatc gcattgcgag cattgtcaca tcttgtgttt
    16621 gcgttgattg gggcttggta cttgcaaaag catccgagta tcgttttaaa aaatggacag
    16681 tttacttttg ctaattggcg tttccaaggg ttcaattttg tgatcggtgt gattcattct
    16741 ttagcagaaa tgctagtagt cagcatcttt tactttattg gtaatatgcc tgaaacatat
    16801 tattctcaag gatattttta tacagtattc atcttgatgg ggattggtgg gcttatccac
    16861 agtttagttg attataatat tgcttatttt atagcaggaa cactcagcaa atattttgat
    16921 atccccgtat ttacggcagc aaaaaaagaa aatcggaaag agtcgattca agggtacgcg
    16981 aagaaaacga catattagac atgatctctc aaaaaaaaaa ctctcatcta gtaatggata
    17041 ataaatcctc taagatgaga gttttttgtc ctaaaaagag ctgcaccaac atcttctggc
    17101 acagctctaa cagggggcaa tttatggaag tttgatgact tgttctgttt ctttatcaaa
    17161 gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt tcgcctggtt tatggaaatc
    17221 tcttgcatct acttttgaga tgaactcagt agaaccagtt tttgtgtaaa gcatcgtttc
    17281 agctccaagt aattcggaaa cgacaacttc tgatttcact acggcttcag gtgaagcttc
    17341 aagggctact tgttcactgt ggatgtcttc tggacgaata ccgaagataa cttcttttcc
    17401 ttcataacct ttttcaacta atactttgtt ttttccttca ggaatacgaa gcttaagccc
    17461 atgtccatct gtgataacac catttttcaa tgtcacatta aagaagttca tggcaggaga
    17521 tccaataaag ccggcaacga acatattgac gggtgtatca taaacttctt tcggtgtacc
    17581 gatttgttgg ataaatccgt ctttcatgat aacgattcgg tcagccatag tcattgcttc
    17641 tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga cgctggtgaa gtttggcaat
    17701 ctcagcacgc atggctacac gtaatttagc atctaaattt gataatggct catccattaa
    17761 gaagactttt gcatcccgaa cgattgcccg tcccaatgcc acacgttggc gttgtccacc
    17821 agataaagca gctggttttc gtttcaagta ttctgtcaga cctaagatct ctgctgcatt
    17881 gtccacacgt tttttgatgt ctgctttatc atatttacgt aatttcaagc caaaagccat
    17941 attatcaaaa acagtcatat gaggatacaa tgcgtagttt tggaaaacca tggcaatatc
    18001 acggtcttta ggtgccacgt cattcatgac ttttccgccg atatttagtt ctccttcaga
    18061 gatatcttcc agtcccgcga tcattcgcaa agtagttgac tttccacaac cggaaggacc
    18121 aacaaaaacg ataaattcac ggtctttgat tgatagatta aaatcagtta cagaataatt
    18181 ttctgcattg tcatattttt tatatacgtt tttcagagcc atttctacca taatacgttc
    18241 cacactttct tttttagttt tattaactat ttaaagtata aatgaaagcg ttaactaact
    18301 tcaatgtcat gttgaacaag aattttttct tttttttgtc aatgtgctag ttttaagctt
    18361 taataaatat ccataagaaa taaaaactga tacactggag taaataagta tgttaacaat
    18421 atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct catgaccgaa aaaaacctat
    18481 gatggtgaaa ttagcaacag cgtataaaac aatattggaa aaacatgaac tttttgctac
    18541 cgggacgact gggcagagga tcacggaagc aaccggcttg ccgatccatc ggtttaaatc
    18601 cggaccgtta ggcggggatc agcaaattgg agccatgatt tcggaggatc agttagattt
    18661 ggtgattttt cttcgagacc cgctggctgc acagccccat gaaccagatg taacagcact
    18721 tatccgttta tctgatgtct atgagattcc gttagctacg aatatcggaa cagctgaggt
    18781 gctactaaga ggactggaag ccggcttcct agattttcga agaatcgtcc atgaaataga
    18841 caagcatcca ctttcatttt gatggataag cgtatggaaa aataacgttt aatgttcgcc
    18901 tatatgtagc tataaaagct aaacaaactg ataatatgcg atataatacg aacgtattgg
    18961 ataatataaa caataatatt agtttttcgt atttgcagta aatgtgaaaa tatgctatag
    19021 tttctgtggg aaaagatccc aaaaataata cagagtagaa aataaagcgt taagtgctaa
    19081 gaggatggga tgttgcctct tggacgaagg acgtaatgtt cgcggtttta ttttcgcatt
    19141 cgctgcatga tacgatgtag cagctcttaa aggagatgct aaaaatgaag aaacaccggt
    19201 tagacgcacg tatgattgct attatgggct tattgattgc attgatggtc actttgtccc
    19261 gactggtagc aatcgagacc ccatttatta aaatcagtgt cacatttatt ccacaagtga
    19321 tcatgggcat tctttttggt cctttttggt cagggatagg cgcagtttta gctgatttag
    19381 taggaatggc cctcttctcg aaatctgcat tctttattgg atttacattg aatgcattta
    19441 ttgaaggagc aatctacgga ttctttttct atcgaaaaga gatcacttgg aaaaatgcca
    19501 ttctggctac gcttagtgtc acgctgatca tcaacttatt tttgacacca ttatggcttg
    19561 cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg ggcgccccgc ctgttaaaaa
    19621 cagtaatatg gttaccgatt caatcaattg ccatttatta cgtaggacgt agtattcctt
    19681 acaaaaaaat tttaagaagt ttagcgatcc acgctaaata aaaaacaagc agtcttccca
    19741 tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg cttgatagag ccgtcggtaa
    19801 taaccatctt tttgaagcag ctcatcatgg gtgcccattt cgacaatagc tccctgatcc
    19861 atcactaata tctggtccgc atttttgatt gtggacaggc gatgggcaat cacaaaactg
    19921 gtttttcctt ccatcattgc taagaaggct tgttggattt ttttctctgt caatgtatcg
    19981 actgagctgg tagcttcatc taagatcaac atagggggat tactgatcat cgttcgagca
    20041 atcgtcaaca gttggcgttg tccatctgat atctttaacc cttgaccgcc aatcttagta
    20101 tctaattttt ccggcaaacg ttctacaaat tcatacatat aagaggcttt tagggcatca
    20161 ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa gattttctcg caatgtactg
    20221 tcaaatagcc aagtatcttg taacaccatt ccaaaacttt tacgtaatgt atcacgttgg
    20281 atagagcgga tgtcatggtc atcgactgta attgttcctt ttgttgtgtc ataaaaacgc
    20341 atcagcaaat taacaagtgt ggattttccg gcccctgttt ttcctactat agcaatggtt
    20401 tgacctggat aagcagtaaa attgaaatct tcgatcaaac gctgcttggg gtcataagaa
    20461 aaagaaacat gatcaaaaga gacttttcct ttgatatttt tgggttccaa ggcataggca
    20521 tctggcaaat caggcttttc ttctggctga tcgatcaaat caaatgctcg ttctagacca
    20581 gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt cgataaatgg tttagaaaac
    20641 tgacttgagt agatcgtaaa gctggaaatc acaccaacag tgattgacga accactcttc
    20701 aatgccaata ggccgccaac taaaccaatc gataaataag caaggtgatc tacaaaacga
    20761 gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt gtcctttttc atatagatca
    20821 gcatttagca gttcaaattg ctgttgacta gcttcttctt gctgaaaagc tttcacgatt
    20881 ttctggttac cgattcgttc ggtcacaaag cccgagatat ccccaataat cctttgctgt
    20941 ctatcgaaat ttgtttgtga agcacgagca acgatccaac tcacaaggaa aataattggt
    21001 gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac gcatcataaa aataaacgaa
    21061 agaatgatga ttgccactcc agaaaataat tgattgaagg ccgcagaaat tgcaatggaa
    21121 atattgtcca tatcatttgt aaatcgactg acgatatttc cgtgggagtt ttgatcataa
    21181 taacgaagtg gcaatcgatt cagatgagca aatgcatctt ttcttaattt tgcaacggat
    21241 aagtaagcta accgattgct taacacttgg atgagccatt gactgactac gttgattaaa
    21301 acgataccac cgaatagaat aagtaaatgg aacagtcgat tgaagcggac ctgtccttct
    21361 ccaatcattt gatcgatact ttgaccaatt tgtaacgtca tcacaactgt tgcaatccca
    21421 ttgatgattc ctagtatcac agctagccaa atgtcttttg cataggcagt aaggtaagga
    21481 atgaatcggc ggagcgtctg caaggataat ttttttactt tcataactat tcttcctcct
    21541 gtgatgcgac gatttcctga tattctgctg attgagcaag taattcttca tgggtaccct
    21601 gagcagcaat cttaccttga tccatcacta agatatgatg tgcttcttga atagagcgga
    21661 cacgttggga gataataatc actgtactgc caagataagt ctgtagcgcc ttacgcaaat
    21721 ccaagtctgt ttgataatcc aacgcactta aagagtcatc taaaatcagt aatgctggct
    21781 tggcaatcaa agctcttgca atcgtcagtc gctgtttttg tccgccagag aaatttttcc
    21841 cattttccat gacttttgta tctaaaccat ttggaagctg acggacaaat tcttctgcct
    21901 gagcgatttt cagagcttcc caacattctt tttctgtggc gtctttctta ccccattgaa
    21961 ggttttcacg aatagtcccg gtaaatagta cagaagtttg gggaacaaga gcaatttgct
    22021 gacggagatg atccagctgc caagaacgaa catccattcc gtccattata actgtaccgg
    22081 aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt cgttttcccg cttccggttg
    22141 gaccaatgat tccaaggact gtatttcgct tcagctgaaa tgtaatattt tggagagaaa
    22201 gaccagaatc aggagtataa cggaagtcaa catggtcaaa agcgataatt gcttcagaag
    22261 aaaaatcggg agtagtactg acctcagctt caagaatcga attttctgtg tctagcacct
    22321 ctttcacccg atttcctgaa gcttcggcac gtgtgaagat cacgacgagg tttgaaacaa
    22381 cgatcagtgc caaaagcatt tgattcatat aattgatcaa agcaagaact tgtccttgtt
    22441 gcaagctacc aatatttact ttgatcccgc caactgtcag caaacagatg atcccgacat
    22501 tcatgatcaa ggttgtagca ggtgataata gggcagagat attggaaaca cgtaaataat
    22561 tgttcgctag atcatttgtg cttttatcga acttctctgt ttcttttttt gtctgcgcaa
    22621 atgcgcggat gacacgaaca ccgctaagat tctgactaac gtgttcattg agctgatcta
    22681 gtttttcttg cactttttgg tagagaggaa cggttttttt gatgataaaa aataaaatga
    22741 ttgagaaaat aggcaataat gctaagaaaa acagacctac ttcccagtcg atcacaaaag
    22801 ccatgaccac tgacccgata cttaaaaacg gtgctctgat caataaacga atgaccattg
    22861 ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg ggtgatcaag gtatctgttc
    22921 caaaatgatt caactcgttg tgagagaaag tattgatttt tttgattagc tgattacgta
    22981 actctgtacc aaatccttgt gaagctactg aagcatagta ttggcaaatg atagcacaaa
    23041 tcagaccaat cacagacata gcgaccatcc ataagcccat ttcaatgatt tttcctcggt
    23101 cattcatttt tagtccatta tcgatcaagg aagccatcat taaaggcagg acaagttcga
    23161 agacagcttc aagaaatttg aagaacgggc ccaaaatgat ttgcttacga tagtcttttg
    23221 cataacgtaa taactgaatc atatgattcc tcctattatt tatttgtcca caattgaaaa
    23281 aacaagtact accacattgt agcttaggaa atttagaatg aaaagagaac aagccttttc
    23341 ataaatgaaa gttttattat gaagaagctt tagaaattga atagactatt ttttttaaaa
    23401 aaaagaaaat gagaaaa
//
LOCUS       DARBFO010000034        23065 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000034 DARBFO010000000
VERSION     DARBFO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.035"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(111..566)
                     /locus_tag="SCK51_001468"
     CDS             complement(111..566)
                     /locus_tag="SCK51_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252104.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(720..1928)
                     /locus_tag="SCK51_001469"
     CDS             complement(720..1928)
                     /locus_tag="SCK51_001469"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEG1252105.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2040..2372)
                     /locus_tag="SCK51_001470"
     CDS             complement(2040..2372)
                     /locus_tag="SCK51_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEG1252106.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2520..3395
                     /locus_tag="SCK51_001471"
     CDS             2520..3395
                     /locus_tag="SCK51_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1252107.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3477..4058)
                     /locus_tag="SCK51_001472"
     CDS             complement(3477..4058)
                     /locus_tag="SCK51_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEG1252108.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4064..5323)
                     /locus_tag="SCK51_001473"
     CDS             complement(4064..5323)
                     /locus_tag="SCK51_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEG1252109.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5325..6371)
                     /locus_tag="SCK51_001474"
     CDS             complement(5325..6371)
                     /locus_tag="SCK51_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252110.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6606..6881)
                     /locus_tag="SCK51_001475"
     CDS             complement(6606..6881)
                     /locus_tag="SCK51_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEG1252111.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7124..8434)
                     /gene="der"
                     /locus_tag="SCK51_001476"
     CDS             complement(7124..8434)
                     /gene="der"
                     /locus_tag="SCK51_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEG1252112.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="SCK51_001477"
     CDS             complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="SCK51_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEG1252113.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9980..10660)
                     /locus_tag="SCK51_001478"
     CDS             complement(9980..10660)
                     /locus_tag="SCK51_001478"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEG1252114.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10709..11344)
                     /locus_tag="SCK51_001479"
     CDS             complement(10709..11344)
                     /locus_tag="SCK51_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1252115.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11410..12813)
                     /locus_tag="SCK51_001480"
     CDS             complement(11410..12813)
                     /locus_tag="SCK51_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEG1252116.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12800..13831)
                     /locus_tag="SCK51_001481"
     CDS             complement(12800..13831)
                     /locus_tag="SCK51_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1252117.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13862..14083
                     /locus_tag="SCK51_001482"
     CDS             13862..14083
                     /locus_tag="SCK51_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEG1252118.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14248..14985)
                     /locus_tag="SCK51_001483"
     CDS             complement(14248..14985)
                     /locus_tag="SCK51_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEG1252119.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15138..16478)
                     /locus_tag="SCK51_001484"
     CDS             complement(15138..16478)
                     /locus_tag="SCK51_001484"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEG1252120.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16715..18238)
                     /locus_tag="SCK51_001485"
     CDS             complement(16715..18238)
                     /locus_tag="SCK51_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEG1252121.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18616..19227)
                     /locus_tag="SCK51_001486"
     CDS             complement(18616..19227)
                     /locus_tag="SCK51_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1252122.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19350..19475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19557..20273)
                     /locus_tag="SCK51_001487"
     CDS             complement(19557..20273)
                     /locus_tag="SCK51_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEG1252123.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="SCK51_001488"
     CDS             complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="SCK51_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEG1252124.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20832..21629)
                     /locus_tag="SCK51_001489"
     CDS             complement(20832..21629)
                     /locus_tag="SCK51_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEG1252125.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21599..21970)
                     /locus_tag="SCK51_001490"
     CDS             complement(21599..21970)
                     /locus_tag="SCK51_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEG1252126.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="SCK51_001491"
     CDS             complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="SCK51_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEG1252127.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6468 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt
       61 agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa
      121 aattccccgt atacatcttc tacacgttga tctggattga tcacgatata caagttacct
      181 gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct
      241 agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg
      301 atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata
      361 tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga
      421 tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc
      481 caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat
      541 tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg
      601 tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa
      661 tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt
      721 taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt
      781 tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt
      841 tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata
      901 ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc
      961 atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc
     1021 aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt
     1081 tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt
     1141 tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca
     1201 gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct
     1261 gtttcaataa atgcatggat tcccgcggcc ccttcatttc ccaagagcat cttgatgaat
     1321 tccacattta tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg
     1381 tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc
     1441 attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt
     1501 tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca
     1561 aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg
     1621 acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca
     1681 tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt
     1741 tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg
     1801 attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct
     1861 gcattctcta attttttcag aactggaatt gccttttggt attccagagg taattgtttt
     1921 aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca
     1981 tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt
     2041 tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt
     2101 tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa
     2161 acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct
     2221 ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc
     2281 tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc
     2341 tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa
     2401 aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt
     2461 aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata
     2521 tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg
     2581 catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga
     2641 tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca
     2701 acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg
     2761 ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa
     2821 tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag
     2881 gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga
     2941 ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg
     3001 tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt
     3061 cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc
     3121 tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa
     3181 gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct
     3241 atatggtcgt tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg
     3301 aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt
     3361 tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga
     3421 ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa
     3481 ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta
     3541 acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt
     3601 tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt
     3661 aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag
     3721 atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc
     3781 tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc
     3841 tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga
     3901 tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca
     3961 agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac
     4021 gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt
     4081 catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata
     4141 attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga
     4201 aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa
     4261 agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt
     4321 cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat
     4381 tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa
     4441 gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa
     4501 gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa
     4561 tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat
     4621 taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat
     4681 tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta
     4741 aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct
     4801 gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg
     4861 ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg
     4921 gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc
     4981 cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct
     5041 tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt
     5101 ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa
     5161 tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata
     5221 tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt
     5281 catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc
     5341 tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt
     5401 tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc
     5461 aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga
     5521 tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct
     5581 tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt
     5641 agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg
     5701 ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga
     5761 gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca
     5821 caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc
     5881 tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca
     5941 cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc
     6001 gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc
     6061 agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag
     6121 cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca
     6181 caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac
     6241 aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt
     6301 agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa
     6361 tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc
     6421 tagaaaagat aagagcaaag ctcacctcat aacttggaaa taaataaaaa ttaaataaaa
     6481 tacatgtgtt ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt
     6541 ttctgctagg aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa
     6601 ctgtcttatt taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta
     6661 gctggaattt cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg
     6721 cgaacttcaa agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct
     6781 tgaatagttg aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta
     6841 gctgaagcaa ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc
     6901 acaagagata tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa
     6961 aaagaaatat tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata
     7021 gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac
     7081 tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag
     7141 ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga
     7201 aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg
     7261 gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg
     7321 ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa
     7381 gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag
     7441 tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct
     7501 cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga
     7561 cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc
     7621 gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca
     7681 ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac
     7741 cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg
     7801 cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat
     7861 tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt
     7921 cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa
     7981 tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt
     8041 catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt
     8101 tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc
     8161 cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga
     8221 tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt
     8281 ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt
     8341 cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg
     8401 ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa
     8461 tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa
     8521 gtatttttat tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc
     8581 agctgctttc atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct
     8641 ttttcttcta cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta
     8701 ttttcttctg gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt
     8761 tcttctaaag ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact
     8821 ttcactttca cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt
     8881 gaaatttgag agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg
     8941 aagcttgtca aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg
     9001 atttctgacc aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca
     9061 ggattgatag acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt
     9121 tttgctacat gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg
     9181 tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca
     9241 tggatagttg aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta
     9301 tgcgataaga tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt
     9361 ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca
     9421 aaaccacgta caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct
     9481 tcgatgacct tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct
     9541 aaacggcgtt ttgaaagtag gtagctgccg ttttctttat ctttgccgat cgtactgata
     9601 acgactaaat ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa
     9661 gttgataatt cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc
     9721 acttgtttgt cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct
     9781 tgtacgcttt tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct
     9841 gtcataacac ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt
     9901 aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt
     9961 tttcattttt tttcttaatt taaaaaagaa gataaccttt tttcaaaaca acagctttta
    10021 ttgctgcaac gacttctgga atcgatttgc cagtcgtatc gatacgaaca gcatcctctg
    10081 cttgcttcag cggagagacg tcccttgttg aatcataatg gtctctttcc tgtatctctt
    10141 tcgtcagtgt ttcaagatca gtcggaattc ctttttcttg gttctctttg aaacgtcttt
    10201 ctgcacgctc acttacgctt gctactaaaa agattttcac ttctgcattc ggtaatacag
    10261 ctgtcccgat gtctcttccg tccatgacaa cgccaccgcg ttcccctatt tttcgttgga
    10321 cagcaactaa ttcttctctg acttttccat gtgcagagac ttcagaaaca gcttttgtga
    10381 catctggttg acggatttct aaagtcacat cttttgatgc tatgaataca tgctgtccat
    10441 cttcttgttg cgaaaaagag ataggatact ttttgatcag cgaaactagc ccttcttcat
    10501 ctgaaaaaga tacattgttc gtaatagcta aataagttac tgcgcgatac atggcaccgg
    10561 tatctgtata aatataattc aattcttttg ctaatatctt ggcaacggta cttttcccag
    10621 atgaagcagg cccgtcgatt gcaatactga tttgtgtcat gatataactc ctttttctca
    10681 gaaaaaaagc cgcaaagcgc ggctattatt atttaattct taattcttga ccagggtaaa
    10741 gcataaagtt gtttggatct aagccattca attcaaacaa ttgatcagtg gtgatccctg
    10801 cacgctcagc cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg
    10861 cagctgatga agaaggtgtt gtttctgctg cattgctgct tgaatcccct gcttgataag
    10921 ttgcctcact tgaggaagtc ggtgttgtct ctgctgcaat actgctttct gacgttacac
    10981 ctgtatcttg agacacagta gattcttccg cgattgtact agattctact gttgaagtct
    11041 cagttgactc ctcttttgtt gtacttgtag aactttctac tactgatgtc gaagtttcag
    11101 aagaagttgc tgctgttgca ttgttgcttg atccatttct gatccagaaa taagcacctg
    11161 ctggaatagc gatacataag accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc
    11221 ctttcttttg ttgtctctga gaacttcttg atgaaacttc atcatctgta tcgtagattg
    11281 gttgttccca tggttcttgt gtttcgttat tttctgtaga ttgatgtcta tcttttctgc
    11341 tcactgtttt acctcctaaa ctctatcgtg tgtattatac cataggaaaa agtgtcttgt
    11401 cctaaaaaat caatgttcca tgtttttttt aaataaattt agtaaaatcg tttcccaatg
    11461 aagttgttca gtcttagaaa aaagcttcct ctttggcaga agttctgctt ttgctccatc
    11521 aatatcacaa caattctgcg gcttttcgga gagtttttcg ccaaaataag ctaagatgaa
    11581 ttttctgcgg cagttttctt cattaatata gcgaagcatc tgctgcaggc ggaattcttt
    11641 ttctttttcc tgacgtttga gccgttccac ccaattttct ggtttttcag actgctggat
    11701 cagctccatc catttttttt gcagctcatc aaagggagct tgctttgtgg catattccaa
    11761 ataaagctca aaactttgtc gctgttctct agaaagttga ttaaagaaat aatggatacg
    11821 ttcatcccca ctttgataaa aaagaattgc tgcactttct tcctgatctc tgcctgctcg
    11881 accgatttct tgtacatagt tttctagact gtctggcaaa tcataatgga ccacataacg
    11941 gatatcagat ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa actgcagctg
    12001 attttttaca aactgctgtt gaagttgtct tctttgagct gcatcaagac caccgtgata
    12061 gtatccaact gtaaaacgtt ccctgaataa atgatacaat cgttcgactt ctttttttgt
    12121 tgcacaataa atgattgccg ctccttgtgc tctttccatg aagtgttcaa gttcttgttc
    12181 tttttgctgc gttttccgaa caaaaagcga gatattttgc cgattgactg attgtctgat
    12241 ctcttttggt ggtcgatcaa atagtacatg acgtatatct cttgctacta aatctgttgc
    12301 tgtcgccgtt agtgccagtg tgaccgggtt tcctaaaagc tttcggattt ttcctaactg
    12361 ttgatattct ggacggaaat caactcccca ttgagacacg caatgagcct catctactac
    12421 atataacgca atattttgtt ttttcagatg ctctaaaaga gaaggattag tcaacatttc
    12481 gggactcaaa aacaaaaatt tatattgcga tagatgacgg aggacgtatc ttctttcaga
    12541 ttgcgaaagt aaactattga aagcagctac tcgtttctcc cctcgttttt gtaattgcgt
    12601 cacttgatct tccattagag aaagcagcgg tgacacgatg atgaccagac cttccattaa
    12661 ataccccgtc aattgatagc aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt
    12721 atcttctcca tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa
    12781 tccaaaatat ttttttaatt catttattaa cattctgtat cgcttccttt ctcaatagat
    12841 agatttgata caaacgaaat tctccataat ctaattgtcc atctacattc cattcgctaa
    12901 agcgccactc tcgcacggaa tccttatttg gttccaaacg ttcgattgtt tctgcagaga
    12961 gaatccactc gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga tgatctgtta
    13021 ctgttcctct ttttaaatga cggattgcca tgatctggtc aattgtctga ccattcataa
    13081 acatctgctt ggtgatcatc atgctttgat tgaaattttg ctgtaataat gaatcgatca
    13141 aagcgtgcca aagcgaatct ggccgttttt cgatttgtgc taacaaaagg tggatacatc
    13201 tgctttgttg cagataaacc tctgactcat cattgttttt tgccagctga taaggaagca
    13261 gccctgttcg ctcatttcca gataattggt tagctaaaaa atctgctgct tcttctggta
    13321 tctccgaaaa cagctgcacc aaactctcat acgcagtatc aatcaaaatt tcacgtggca
    13381 attggctttc actaagccat ttttttagtt ggaaagtata aaaaggactt gtctctgctg
    13441 gaagatagtc ttgaataccc gttgccagat tagaaattac ttgaacagca aatttaatga
    13501 gccgccaact agtttcccct gttctgccat agcggtcaaa acgcaaacca gtatgttcta
    13561 tttgatgttc ggacaacata tcagcccctg caggcgttag ctttgcttca ttttcattta
    13621 tttcgatcca accttgctgc gcaatttttt gcaaaatttg ataaaactta tcctgcttca
    13681 attctggaaa actgttatgg ataaacaaaa gttcgtgggt aaatccataa ataagtactg
    13741 aagtcgttcg ttttcccaca aggagttggt aaagagaact aatccgcagc ttgtgtcctg
    13801 atgaaaatag cgataaaata aaaacaatca tattattctc cttaagttag gatggttaga
    13861 aatgtcacta ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat
    13921 cgaagcgcca gatattttcg attatgatga agaaggaatc gttttatttg cccaagaacc
    13981 agaggctcat caacagttcg tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca
    14041 aaaatgtcca gttcgagcga ttttattaga aaataaaata taaatataaa cgaaacttta
    14101 aaaacgtaca ctaagttctc taaacttgaa aataagccgg gatctgtttc tcttgttccg
    14161 tgaaatcccg acttattttg aaagattcat ttgctatcta tagttaattg actagttttt
    14221 tcacggaccc tataggaccc atttatatta tttcaacttt ttcagccagt tttccaatgt
    14281 attgacgatc acagtttgct ggtctgcgtt agaaatttct gcctctccat ctcctttttg
    14341 ctcaccatag ctgccaaaac ctccatgatt tccgccattg attgatacat actctgttct
    14401 ttccggtaaa tactgcttag cttcttgata agcttcctga ttcaatactt tatcttttgt
    14461 cgccgtgatc gataaaacag gaacagctga ttctgtcaat gttccttttt tgtcgggata
    14521 acttgccatg aagaataccc cttttaaatg tttgtctgaa tgattcttat tgacataacg
    14581 acttgccatt acgccaccta atgaatggcc accgatcaca taatcggaat ttggaaaatc
    14641 ttctttgacc ttatccgctt tgtttccgcc catcactgct agatcaagtg gcattttgac
    14701 aatagcgact gaaaagccgt tttttgctaa atcttgcgcc caaatactat aactttctgg
    14761 ctctacgaga gcaccaggat aaaaaataag taatggagac tttccatgat tctttttctc
    14821 tccttcaaaa aataagtaat cttctttgtt ttttccttct tttaccaaca cttgcgcttc
    14881 tgacgatggt tcgtacgtat tttgctgtat atagtaataa ccggctccac cgagtaatcc
    14941 taaaatgatg actaagccaa ttagtattcg tctaaatatt ttcactttct cccacctttt
    15001 ctatctgagt gacccctttt tcttaatcaa gtatgattat accttaaaaa aataagagag
    15061 aaatgcaaac tttccagcat caaataaaaa aagacggaat atccatttga atatccgccc
    15121 tttttgtatg tttattttta tttagcacat aaacgataca cagcttctgc ataaatttcc
    15181 attgctttat acatactact taattcccat cgttcattcg cctggtgcat aaagtcagga
    15241 gtatcaggga acatcgcacc gaatgccaca cactggttca tcgtacgagc aaaagtagcg
    15301 ccgcctgata caaacggttc agtcatgtct ccagtaatat cgcgatatgt ccccaataga
    15361 tttttcacta actcactgtc tagtggcacg tataatgaat caaggaagtc aaattcttca
    15421 taagtcaacc catatttttc tgctgtttta gttaatttag ctactaaatc atctttcttg
    15481 aatgtaacag ggattctcat atctacaccg atttttgttt gttctggtgt gatttccaaa
    15541 cttgcaaagt tcatcgttag ctcgccggat tgttcatctt ctgttttgcc cacgacagct
    15601 tcaccagttg cattttcttg aactaatttt cctaagaaat tgattgggcc aaaatcaaat
    15661 acttctgaaa gagcaattgc cagacgagaa atagcattca ctccttgtgc tgcatctttc
    15721 gcgtggatac ttttaccaag aacgacgatg ccttcacctt gttcttcgaa atcgaattcg
    15781 tgttttttca atgcttcttt tacttcagaa agcttttctc cagagtaagg agctgcatca
    15841 ggaacaacat tcaacgctcc tccagctttt acagaaaact cgttcgttcc tggtcctgtc
    15901 aagtaagctt gcaataagcc tttttctgca taaattaaag gaaattcagc atctggcgca
    15961 aatccttgcg taattccttc ttctttttcg ttatattttt ctaaacaacg ccataaattt
    16021 tcttcatctg taccaaagat aaaacgaata cgtgtcttga attccacgcc agcatcaatc
    16081 aaagctttga cggcatacat tgccgcaata gaaggacctt tgtcatcttg ggatccgcga
    16141 ccaactaacc agccgtcttt tatagttgga tcgaatggtt ttgtttccca attattttcg
    16201 tcccctgctg gaacaacgtc catatgacac aagataccga ataattcatc tccagagcca
    16261 atctctgaat aaccgtaata gccttctgga tctttaaatg ttctaaatcc tagcttttca
    16321 ctgatttcca atactttatc caaagcttca atcacttttt taccgaaggg atgtccttgt
    16381 cctgaatctg cttcatctag tacggaagga attcggatca attcgctcaa tgattctaat
    16441 gcttgttcat gatgttgttc tgtcacaaat tttttcatga tattatcctc ctaatatgtt
    16501 aaaaagggtc atgtcaaatt cctccgacac aacctcttct tctcaaataa tgacgtttta
    16561 agcctgctcc atcagcaagc agaatgttct taacaaatga tgaaagaaaa aacactcact
    16621 gaatggagct attgacttgt ccacgtgctt atcgatagcc gtttatcggt ttcttcattc
    16681 ttatgataga ccttgtacct gtatttgtct aggtttagaa cattgctgct acacctagga
    16741 tcacacagat ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc caccacactg
    16801 aaacatcgaa acgagcaatt gctacagcac ccattacgat agcagatgtt ggtgtaatta
    16861 agtttactaa accagatgca gattgataag cagtgattac taaatcctta ccgacgccgg
    16921 caaattcacc cataggtccc atgattccca ttgttgcagc agctaagccg gaagttgaag
    16981 gaatcaagaa tgacataggg atatagaaaa taaatgtaat aatgatgaat acacttgaag
    17041 ataatgaact taatccttgt tcgccccaat gtaagatggt gtctgtgatt aatccattgt
    17101 tcataaccac ttggatacca cgagcaacag ctactacgat tgctacaccg atcaaatcag
    17161 aagcaccagc taaaaattca gagacaaatt cagactcttt catgccataa gtgattgcta
    17221 ctacgatgga cattactaag aataacatag taatttctgg gaagtaccag tcacctaatg
    17281 gaagcatgct tttacctaga accgtaccta gtaccggaag gtttgttaac cagtttgtca
    17341 atgattcaaa aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa ctgatgatca
    17401 tgataccaaa agtaataaag aacaatacat taacatgttt ttgttttttc gtgattactt
    17461 cttgacgacc gattgcttca atatcgaaat gcttcatatc ttctttacgt tggttgtata
    17521 ctaatgattt tgttggatct ttttcgatct ttgatgcata acgatacaca tatacaatag
    17581 aaatagctaa caaaataacg aacatcaaga atcgaagacc aataccttca cccatcgaaa
    17641 tcccaacagc ttgtgaagct acgcctgttg caaaaggatt taccgttgaa gctagacagc
    17701 ctacttgaga ccctactaat acgattgcta cagctactac tgtatcaaag cctacagcca
    17761 tcatgactgg gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag
    17821 agccgcctaa tgcaaataac agcatcaata ctggaataag cattttttct tttcctttga
    17881 atttcttcac aactgaagca atcccggcat ctagtgcccc tgttttgttg ataataccta
    17941 aaaatccacc aacaaccaaa atgaagaaag agattgggat tgcagcaggt gtttctttag
    18001 ttagctcccc cgctggtaca cctagcatcc cattgattgg cgccatgaaa atatcccaca
    18061 gcccttgagg attttgttgt actcgttcat atgatccagc gacgatgttg cctgcatcgt
    18121 ctacactata gtgcccagct ggtataaacc aagtaagtac agcgattact gcaataataa
    18181 taaataagac cgtatacgct gaaggcattt gaaatttttt cttttttgtt tgttccatct
    18241 tcttcttcct ttcttttccg ttagagacaa caattttggc tctaatgtta ttttatcaaa
    18301 aagagtatca gaacaaaaat aataacacgc attttgtaaa acttttgttc aaaaagttcg
    18361 tttttcccta ttccctcctc ttttttcagt tttttaaagc tttcttcttt ttcattgtag
    18421 cgtttccttt tttttataaa caaacatttt cgtcttttaa aaggagaaga atttcacatt
    18481 tgcaaagaat tgattgattt ttatgcataa atcaaaaaaa tactcaaaac aaacaaataa
    18541 aacgtttaca ttattgcatg tcttgtattt ttttccaaaa aaaaagacca agaaatttct
    18601 tgatcttttt gatatttatt tgactgctgt atgacgttgt tcgaactgat gttgttttcg
    18661 acttaaccat ggaagaagtt gcgcatgcag cactaaaaag acagcactca cgatgaatcc
    18721 tttgatcaag ttgaaaggaa cgatcccaat cagtacatag tttgctaaag acatgcccag
    18781 catctgtgtt gcgttcagcc ctaaaaacat taagtaaaga ggcgtaataa cgaaataatt
    18841 tgcgatactc ataaaaatag ttagagcgat ggttcctgta atgacaccta aaaatttgtt
    18901 tcttccttta tgctgtttct ttacactaaa gaaataataa atcgggaatg taaagatcgt
    18961 tgttgataag aaactagcag tgtctcctac caaattatcc ggagaaaaac ctgtagtcag
    19021 cagatgtaat actgaacgca agaacgctgt tgcaactccg gccgctggac cgaataagaa
    19081 catgctgatc aataccgaga tatcactaaa atcgatcttc aaaaaactaa aagccggcat
    19141 gatcggaaaa gcaaaatact gtaaaactaa tgcgatcgca gcaaacatcg ctactgcaac
    19201 cattttctgt acacgggtgt tcttcatttg aaatcctcct gttaggactc ctcttcagcg
    19261 aactttttga cgtttctctc caaatgaaaa aactctattt tccagggaaa atagagttag
    19321 agttatttga tcaagcgtcg tcaaataagc tctatcttct ttatccagac tatactgtcg
    19381 gtatcggaat ttcaccgatt cagctatttt tttgcaaaat aggtcgtgga ctataaccac
    19441 cggtcgggaa tttcaccctg cccctgaaga cgaaccttat ataatttttg tttacatatt
    19501 cattatacat atctattcaa aaaacgcaac atctaacaat tataaaaagt aagtgttcat
    19561 tttgagtggt tcagcaaact gctgacttct tttttgttca gttcacggta atccccagga
    19621 cgtaggcctt gtaaagtcag atctccgtat ttttcacgtt ttagtttttg cacaggaaga
    19681 ccaactgctt gaagcatatt tttcacttga tgattacgtc cttcgtggat cgtcaattcc
    19741 acgatactgt ggtttgtctt aggatccact gataaaattt ggaatttagc tggagatgtt
    19801 tttcgtcctt cgatctttat tccttttgtc aaaggtgcaa gcatcgattt tgtagcgatc
    19861 ccttttacct ttgcaacgta aactttatcc acttcatgtt ttggatgtgt caggcgctga
    19921 gcaaaatctc catcatttgt cagtagcaag atccccgaag tatcgtaatc cagtcgacct
    19981 acaggataga tacgttcttt taccatcggc aaaagatccg taacaacttt tcttcctttg
    20041 tcatctgcaa cagcagaaat aactccttta ggtttgtaca ataaatagta accgtattct
    20101 tcttgataaa ttgggacttc atccacttcc acagtgtcat gtttcccaac ttttatccct
    20161 aattccctta caacttgtcc gtttactttt acacgtcctg caaggatgaa ttcttctgct
    20221 tttcgacgag aagtgatgcc agcgtgggca attactttct gcaatctctc cattacaatt
    20281 attctccttt attcattcca tcaaagaata gatctggtga cacgtcttct tcaagtgaaa
    20341 tttccatttc ttgtgtatca ggcagttctt ctaatgattt caaaccaaaa taatctaaga
    20401 agtagccagt tgttccatat aatatcggcc gacctggtcc ttctactcta cctttttctt
    20461 caatcagttt atgggcagct agacgctgta ccgctcctga aacctgaact cctcgaatct
    20521 gctcgatttc agctcttgtg attggctgtt tataagcgat gattgctaag gtttcgacgg
    20581 cggcttgaga taatgtgttt gatgtccctt gcgcatatcg cttaagtaag ggagccagct
    20641 cttttttagt cgttaataca taatggcttt ccgtttcaaa aatatgcaaa gctgagtgcg
    20701 agttttgagt gtaagattct tttaattctt tcactaaact tgtgacttct gcttcgctga
    20761 gatttaacag tccgcttaat tcttcattac tgatcccttc ctcgccaaca ataaaaagaa
    20821 gggcttcaag ttcacttaat ttcgtcatgt tcggtatctc ctactgctgg ataaagcata
    20881 attgattcat agttttcttt ttgttgcgca atgatctgcc cttttttcat caattctagc
    20941 aaagccataa aagtagtgac aacttcactt ttagaaggtg actcaaaaaa agaatcgaaa
    21001 gtacagccat gcgcatttct ttttatacgc tcttcgataa ataccattcg ttcttcgatc
    21061 gttgtgtcat cgggcgtgat cgttttttcc actttttgag aaaatttctt acgttctaag
    21121 attttctgca tggcacggat caaatcgatt tttttgactt gtcctcgctt caacggcttt
    21181 tcagcatccg ctaacccatc gatgttcata ggttcttttg tgtaataaag acttcgttcc
    21241 tctgcttttt ctgtcagtct ttcagctgca tatttatatt tacggtattc taaaagctga
    21301 gtgaccaatt cttctcgagg atcttcttcc cataattctt catcgtctgt gccgaattct
    21361 tggacaggaa gcaatgtctt gcttttgatt gccatcaatg tagcagccat cactaagtat
    21421 tctcctgcaa cagctagctc gagtgttttc attgtatgga tataattcat atattgctcc
    21481 gtgatcgtag cgatcggtat atcatatata tcgatttcca tttgctgaat cagatgaagc
    21541 agcagatcga gaggaccctc gaatacatct aatttaatat tgatctccgt caactcattc
    21601 actctttctt ctttttcttt gctagccatt ttccaatgta aggctctgtg atttcagttg
    21661 cacggatgga gacatccggg tacttttcct gtaattcatc taacatttga aagcccatat
    21721 tctcaccgcg ataagacgga ttcaaagaaa tgtcatggat gatcagctga tcgttcgttg
    21781 cccagtaaat accaataatc ccttgaatgt tggttgaacc ttctggcgta aagaaaaaaa
    21841 gttcataatc gtcttcagat tcgtacgtat caatctcctt caacaaagaa gttttacctg
    21901 tcagtttctt atggaaactc aatagtccca tagcaatttt acggttttct ttccggtaat
    21961 gtgttaacat cttctcacct tcctatgact aagcccttgg aaaatgctgc ttataaacat
    22021 ccgtcattct tttcttcgtg atatgtgtat aaatctgagt cgtagagata tccgcgtgac
    22081 ctaacaattc ctgtactgtc cgcaaatcag ctccattttc tagcagatgt gtggcaaagc
    22141 tatgccgcag cgtatgtggt gttacttctt tgtaaatccc agcctctcgt actaactgct
    22201 tcagattttt ccagattcct tgccgagaaa gacctttccc atgattattg acaaatacat
    22261 gcatttcaga tgcgtccttt acaagtaatg gacgtgcttc gtctaagtag cgttccagcc
    22321 attggatcgc ataatcacct aaaggaatga tccgctcttt atctcctttt ccaactgtct
    22381 gtaccagtcc aagagaaaga tgaagatctc ccaatttcaa tccaatcaat tcactgacgc
    22441 gcatcccagt agcatacatg acttccaaga ttgcacgatc tcttatccct aacgtttttg
    22501 tcgtatcagg tgcttcaatc agcttttcta cttctgtcag agtcaatgta cttggtaatt
    22561 tctgtgcttt cttcggcgta tcgatatgtt gcatcggatc atgatcagtg atacgctctt
    22621 gccgtaaaaa ctgatggaat cttctgagac ttgagatcat ccgagtaaca gttgctgtcg
    22681 cttttttttc ttcatgcaac ttctccagaa aacggatgac taaaaagcga tcgacatcct
    22741 gccatgaagc aattttctgc tcctgtagaa aatgcaagta ctgttccaaa tcacgttcat
    22801 agctttgtcg tgtattggcg gacaatcccc gttcaatatt caagtagtgg agataatcgt
    22861 tgatttgctc tttcataata atccccctca tgcctctcta tcatatcaga taccgcttca
    22921 ttctgatagt ctttttccat aatctgagca aagaaaaagc cgtctagaca ctcagacgga
    22981 ctggtttctt ttttcggctc tataatttat gggactgatg aatcagaatt gattcttcag
    23041 ccccattttc attttaggta ttaaa
//
LOCUS       DARBFO010000035        22772 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000035 DARBFO010000000
VERSION     DARBFO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.036"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(144..578)
                     /locus_tag="SCK51_001492"
     CDS             complement(144..578)
                     /locus_tag="SCK51_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252128.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(688..2547)
                     /locus_tag="SCK51_001493"
     CDS             complement(688..2547)
                     /locus_tag="SCK51_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG1252129.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(2665..3825)
                     /locus_tag="SCK51_001494"
     CDS             complement(2665..3825)
                     /locus_tag="SCK51_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HEG1252130.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(4132..5556)
                     /gene="araA"
                     /locus_tag="SCK51_001495"
     CDS             complement(4132..5556)
                     /gene="araA"
                     /locus_tag="SCK51_001495"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEG1252131.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(5572..6270)
                     /locus_tag="SCK51_001496"
     CDS             complement(5572..6270)
                     /locus_tag="SCK51_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEG1252132.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(6227..7888)
                     /locus_tag="SCK51_001497"
     CDS             complement(6227..7888)
                     /locus_tag="SCK51_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEG1252133.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            8106..9506
                     /locus_tag="SCK51_001498"
     CDS             8106..9506
                     /locus_tag="SCK51_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEG1252134.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(9758..10837)
                     /locus_tag="SCK51_001499"
     CDS             complement(9758..10837)
                     /locus_tag="SCK51_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1252135.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(10923..11882)
                     /locus_tag="SCK51_001500"
     CDS             complement(10923..11882)
                     /locus_tag="SCK51_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEG1252136.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(11894..13405)
                     /locus_tag="SCK51_001501"
     CDS             complement(11894..13405)
                     /locus_tag="SCK51_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEG1252137.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(13427..13990)
                     /locus_tag="SCK51_001502"
     CDS             complement(13427..13990)
                     /locus_tag="SCK51_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEG1252138.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(13996..14859)
                     /locus_tag="SCK51_001503"
     CDS             complement(13996..14859)
                     /locus_tag="SCK51_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1252139.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(14873..15769)
                     /locus_tag="SCK51_001504"
     CDS             complement(14873..15769)
                     /locus_tag="SCK51_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1252140.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(15944..17233)
                     /locus_tag="SCK51_001505"
     CDS             complement(15944..17233)
                     /locus_tag="SCK51_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1252141.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(17205..18722)
                     /locus_tag="SCK51_001506"
     CDS             complement(17205..18722)
                     /locus_tag="SCK51_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEG1252142.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            18920..19810
                     /locus_tag="SCK51_001507"
     CDS             18920..19810
                     /locus_tag="SCK51_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEG1252143.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(19883..20707)
                     /gene="nadE"
                     /locus_tag="SCK51_001508"
     CDS             complement(19883..20707)
                     /gene="nadE"
                     /locus_tag="SCK51_001508"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEG1252144.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(20719..22191)
                     /locus_tag="SCK51_001509"
     CDS             complement(20719..22191)
                     /locus_tag="SCK51_001509"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEG1252145.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         6563 a         4820 c         3854 g         7535 t
ORIGIN      
        1 atttactcga ttttgagtaa gtacaaggtt gttgttggtt ataggtatgt cattatcata
       61 aaggattttg atcactctat cataaaggta atattacatt tatattagat aactctagtt
      121 tttattgtta tcaaagattt cttttatttt ttcaacaaca aattagtcat tttttggatg
      181 gcagctgtca attggtctgt cgcttggtct tgttttgctt cagatgtatt gaatagatct
      241 tcatctaccc ctgattcaag atcgatactt tggatcattt cttcacagcc tgctacatat
      301 gaaagataag ctttttcaaa atttttatga atgcccattg cttttgctgg tggacgcaaa
      361 gtactgactt tcttaagcat tgcccgatat ttttttgttc cttcttcaaa taacaggatg
      421 atctcttcaa gagtcgattt gtccagttca cttgtttttc cttcatcaat tgcgtttctg
      481 atcacttcat atttcggatg cattccttct ccgattgctt ctgtttcctt gacgatttga
      541 tcgatggact gcacataata gccaatattc ggtaacatgt tcgttttcct actttctcca
      601 ttgttgttat tatttattct acttgttttt atcctcaaat gacaagtact tgaaaaaatt
      661 taatggtttc tatacagctc gccattctta ttcgtgctca aagaaaccat ctgctaaact
      721 atattgtttc cctgcgacaa tgtcatattg gatcaattga tattcgtcgt aaatctcttg
      781 ttgcgagcga ttgaattgcg cttctttata ccactgtccg ttttgatagt acagttctcg
      841 ttcaaacgcc ggaagttcct gttctaatga taaaagcagc tgataaaagc catttgttgt
      901 ctgattcgtc tgttccatta gattcgctgc aaaataaaac ggactggtga ttgcatcgtg
      961 agtcgtttgc ttttctaatt ctcctttact gtcgaacata agaaattgtg tttcatgcaa
     1021 tgtttgtttg tcattttttg cttggatcgt atctgaatag atccctggaa gatgatcccc
     1081 ccaaaaaaca actaaagtcc ttctagaaag attcttcaat tcttccgtga actgcttcag
     1141 tgccgtgctg ctataggaaa tatcttgcag ataattctct aagtcaagtg tcccacttcc
     1201 atctgaagtt tttgcaggat agtctagatt atcatacttt ccatcgtagg gcatatgtgt
     1261 ctgcatcgtg actaaatgaa tgaactgagg tgttttttct tccttgagaa gtgtcattac
     1321 ttctttgtaa gctgactcat cagaaatata tggattattc tctatcgtat ccgtataggt
     1381 catcgtacgc tcgctgatga actggtcaaa tcctaatgtc tgataaacat cctctctttt
     1441 atacatcgaa gtattgtatg gatggatcgc tgtcgtctgg tatgattgtg cgtttaacgc
     1501 tgaaacgata gaaggaagct gatccatttt tggtacaagc atcgtgtaag gggtggtgag
     1561 ttgcgcatta aataacgcca tagaaaaacc agtcaatgct tcaaattcga tatttgctgt
     1621 tccaccgcca tagttttgag aaagcatatt gccactatat gtttgatcag ctacttcata
     1681 ataatctgcc aatggctcac cagatacttc tacgccgttt aatcgactag gatctgagaa
     1741 actttcactc atcacgaaca cgatgttcgg cgactcttcc tctacagttt tatttttttc
     1801 atcggctagc ttctggtatt tttcagtgat ttctttgatc ttttctttcg aatatccttc
     1861 aggttcatcc attggttcta ccttcagatt gtacaaaaac ccaccgataa aaccagtgtt
     1921 atagtagttc attttctggc tgtaaggtat ccatagtgcc gttttattat atgcttttcg
     1981 caacaaatta tcgggattgt tgaaattact gatataccct agcagtccaa ttgccacaag
     2041 cagtgtcgtt aatcggatca attggaatcg ccttcctttt ttcaggcttc gaaacatgta
     2101 ccagcaaaac aatcctatca cggttcctgc ggcagctaag atcactgtaa acagcatagt
     2161 gccggtcata tctttcagca atccgatttc agtgatcatc ttcaaatcgt ctggataaat
     2221 cggttcttgc cgataactca ttttcatata atttgcaaaa cctaatacac cgatggctac
     2281 gacatacgta agggaaccta aagggacaga tcctgctagt gcaatcagaa aaataagaat
     2341 aatcagcaag acgagacagg ctaagaagaa tttttccgta tgccaagaaa aagcaaattt
     2401 caacgcaagg tctacggata gatcattttg acaccactgc aaaaataaat tactaaaaac
     2461 aacaagaaaa aagatactga tgattgctgc tgttcctttt aaaatctttt tctgtttggt
     2521 cgaaagtctg cttatatatc ttttcaaatg cttgctcctt catttctatc aataaaagaa
     2581 tgttgcttct cttattttag ttgtacgaaa ttcgaaagta aagaacggaa agaagaatta
     2641 atgaaatcca aatattctct ttctttatgg cctttctggt ttctttaata agcggacctt
     2701 tcgtactcca gaaatttttt ctagctgttg tttcacttga ttgagctgtt caaaatcagt
     2761 actgtctatg tcaatcaata tgtagacatg ctcattctta tggttacgcg atatatctgc
     2821 gatcgctagc tcgttctcat tcagcagaga aaatatctct gctaatatat tggttttctt
     2881 ttgataaaat atagtgaatc gaaacggcgc ttggaaaaac attcgtgcgg atggataatt
     2941 gaccgcttct ctaaccgtac cgaacaaaag ataatctttt aaggcttcaa ctgccaaacg
     3001 tccgctgtct gccaaagctt cctgagtcga tccccctaga tgaggcaaca taagtatacg
     3061 gtcattttct aagaactctt cttctggaaa atcagtcaag tatttagcca gatgttgttt
     3121 ttccaatgct tgcataacag catatttatc aacaatttca cttcttccta cattgataag
     3181 tacagcatct tttttcattt tttctatatt cgcctgatcg atcaatcctt ttgtatcttc
     3241 tgtcaacggc agcatgacta cgatgtaatc cgaggtagaa agaactgttt ctaaatctgc
     3301 ttgccgaaca taatccaact gggcatcgcg gatagaataa cccaagacat ccatccctaa
     3361 gctatagcaa cttagcgcta cttttgtgcc tatcgctcca agaccaagaa gaccgatggt
     3421 tttgccttgc aattctcgtc ccacataagc acttcttttg ttttctgcct gttctaaaat
     3481 attaggacct gttagtgttt gtaccatacg acttgcttcg attatcggac gggaagataa
     3541 gagcaaacag cacaaaacaa gctcttttac tgcattagca tttacacctg gtgtattcat
     3601 gacgatcgtc ccgttttcag aagctttttc aacgttgatc gtgttcaccc caacaccagc
     3661 tcttgaaatt gctagcaaat ctggtgtaag ccattcatct ttcactttcg tactacgaat
     3721 aactattcct tcggctggca aatcatctat tattttgaaa tattcccctt gtaacttttc
     3781 tatctcttgt tctggaatag catcaatcaa atgaacgtga tacataaaac cccctcctta
     3841 ctaacaagta tctcgaaatt tctgcaatta gacaactgtt taccatttga cttcctagaa
     3901 gcaaaaagag cagttagagc agagtaggct gagtgataaa aataaggcta tgacaagtta
     3961 ctgccataag ccttattttc gagagcaaaa tattgagggt gagatatctt gataaatggt
     4021 gttccgttag ctgacattga taataagact aaatttcaaa aaagttgaaa agcaaatttc
     4081 gtcaatctct tcttatttct taaaactaag tggctttttc acaacctctt tttatctaat
     4141 aaccactgtt tccaactcaa ctaattttgc ccacgtttca atttgatctg ttgttagatt
     4201 tagcgagaca acggtatgat gaccgccacc tgcctgaatc catgaatgga caccttcatg
     4261 gaaattcggt ttagttttcc ataacacacg tgcaacaggt aaattaggtg cttcagtcgt
     4321 tggttcaaag gcttctactt cattgatcag cagtttataa tgcgtaccga aatctgccat
     4381 cgatacgact accccttctc ctgctttccc atcaaataca agacgcgccg gatcttctct
     4441 atctcccatt gataaaggag aaacaacgat tcttggtttc gtcgcagcaa gcgtcggatc
     4501 tacttccatc atatgggatt ccaagattgc ttcttggcca ctagctagct cgtaagtata
     4561 atcttccatg aatcctgtat ctttccctcg agccatgatt ttcagcaacc gatcaatcgc
     4621 tgcagttttc cagtcgcctt ccccagcaaa accgtatcct tctgccatca aacgttgtac
     4681 agctagtcca ggtagttgtt tcattccata caggtcttca aaattagtcg taaaagcgtt
     4741 atagcctcct tcttctaaga aacggcgaat accgatttcc tgctgagctt gtacttttac
     4801 atgttcctcc catgtctttt cttcataatc accatagtcg aaggtataca attccttgta
     4861 ctcttcaaat aattgatcga cttcttcgtc tgatacgcga tcgattactt gtacaagatc
     4921 tccgatacca aaataatcaa cagtccaacc aaattgaatc tgtgcttcca ctttgtctcc
     4981 ttctgtgaca cccacgtttc tcatgttgtc accaaaacga gccactttta ttccaaaact
     5041 ttcattatag gctactgcaa catccatcca atcagcaatc tctttttgga tctcaggtcg
     5101 tttccagtag ccaacgacga ttttattttg tttatttaat cgagcattga tgaacccata
     5161 ttcgcggtca ccatgagcgg attgatttaa attcatgaaa tccatatcga tcgttttcca
     5221 aggaatgctt tcgttatatt gcgtagctaa atgaagcaat ggtttttgta gaagctttgt
     5281 cccgcggatc cacatttttg ctggtgagaa agtatgcatc caagtgatca cacctgctac
     5341 ttcttctcga taattgacct ctttcatgat ttttgtgatc gtatcagcag taactgctaa
     5401 ttcttgaagg cggattggat aaggcaattg gccattttca ttcagttcat cgaccatttc
     5461 ttgtgcatgt ttctttactt ctcgcagtgc ttcttcccca tataaatgct gtgatcctac
     5521 aacaaaccaa aattcttttt ctccaatatt caacataatt agccactcct tttatttgtt
     5581 ttgtccgtaa tacgcatttt ttccgtgttt tcgtaaataa tgtttatcta aaatccgttg
     5641 aggcagctct tctgaaaatg aattgagctg tctggtaaat aaattcatct tgcatacttc
     5701 atctagtacg actgcgttca ttaccgcact ttgcgcatct ttcccccaag taaatggacc
     5761 gtgtccgtgt aaaagtaccc ctggaattgc taagggatca attttgcgtt ctttgaatgt
     5821 ttctatgata acttttcctg tttcatattc gtacccgctg tcgatttcct gttgtgtcaa
     5881 aaatcgagca caaggtaccg atccataaaa agtatctgca tgcgtcgtcc ccatcgcagg
     5941 aacatccaat cctgcttgtg cccagatcgt cgcccatgtc gagtgagtat ggacgattcc
     6001 tccgatttga ggaaatgaac gatataaaac agcatgagta ggcgtatccg aagagggatt
     6061 gagtttacct tcaatgatat gattgtccaa gtccacaacg accatgtcgt ctgctgtcat
     6121 ctcttcataa ctgactccgc taggtttgat aacgaaatat cgtgtttcag gatcaaaagc
     6181 gctcacattt ccccatgtat atttgacgag tccgtgttta ggcaagtcaa gatttgcttg
     6241 gtatacttct tctttcagtt gttccagcat tttctatcac tcctttttct tgcaattgat
     6301 gaatcgctgt tttttcaatg tcgatcccct cacggtaacg tctcataaat atctgatagc
     6361 ctgctatttc ttctgctgaa ggtgtgatgg tgattccttg gtcttgtcca aacacgtctt
     6421 ctgccaagaa gttttctaaa gacttcccat gtcgatccat catataggct gctaataacg
     6481 cgattcccca cgctccacct tctccggctg tgtccatcac tgtaactggt gcctccatag
     6541 cagatgctag aatccgctgt ccaacttctg gtgttttaaa aatcccccca tgccccacta
     6601 aacgatcaat ttggacattc tcagatttca atatatccat tccgattctc attgcaccaa
     6661 aagcactggc taaatggatc cgcatgaaat tcgctaaatc aaaacggcta cctggtgtac
     6721 gtacaaataa tggccttcct tcatttactc ctgtaatgtt ttcaccggaa aaataaccat
     6781 aagaaagtaa tcccccgcaa tccgtatcgc cttctagcgc tttgttgaat aataccgtga
     6841 ataatttatc tctgcttatc tccataccca aagaattggt aaattcttca aaaatactga
     6901 cccaagcatt gatatccgaa gagcagttat tcgtatgaac catgcctact agttctcctg
     6961 aaggtgtagt gaccatgtcg atttccggat ggacattttc tagttctttt tctaatacga
     7021 tcatagcgaa ggcggaagta ccagccgaaa tgttccctgt ccgtttggct acactgttcg
     7081 tagcgaccat accggttcct gcatcccctt ctggcggaca aaccgggatt cctggtctca
     7141 aatttcccgt agggtcgatc agataagccc ctgattcgct taacttgcca gcttcttctc
     7201 ctgctactag aactttaggc aaaatgtgat ccaagtcaat cgctacgtgc gctttttcga
     7261 ttaactgacc aaaaatattt ttcatttctt gatggtaggt tttcgttgaa ggatcgatag
     7321 gaaacatgcc tgatgcatct cctacgccta gtactttctc accagtaagc tgccaatgga
     7381 cataacctgc aagcgttgtc aaaaaattaa tttctttcac atgttcttct ttattcaaaa
     7441 tagattgata caaatgagcg atactccatc tctgcggaat attgtattga aatttttccg
     7501 ttaatattcg ttctgcttct tcagtgattg tatttcgcca agtacgaaat ggtactagta
     7561 actgttcctt gctatcaaat gctagataac cgtgcatcat tgcactgaac ccaatcgacc
     7621 cgattgtcgt aagattctcg ccatagattt cgaatacttc tgctgccatt tttcggtaac
     7681 tgacgcgcag tcctttccaa atatcttcta atgaataggt ccagatacca ttttctagtt
     7741 tgttttccca ctcataacta ccagcggcaa taggagtaaa agatgaatca atcaatacag
     7801 ctttgatcct agttgatcca aactcaatcc ctaaagatgt acgaccatct atgacatcta
     7861 acatactttg cgcatttact tccttcataa tgatttaact ccctccttct cgtcaaacct
     7921 gatcaaaccg tctttttagt agcgcttaca atgattattg tatacttttg tacgtacatg
     7981 tcaagcggtt atttacaaga atacaataaa aaacacttgt tttgtacgta cataagttat
     8041 aaaatgaaat tgcttacaaa atctgatcaa ccgccttctc gaaattctag aaggagagaa
     8101 tacttatgaa aaaagaaaaa aaagtttcct catcgtttat ttattttttt ggcgcatttg
     8161 gcggcattct atttggatat gatattggtg ttatgaccgg cgccttacct tttttacagc
     8221 atgattgggg tttggcaggt aaagcttctt tgataggatg gatcacgtct tcggtcatgt
     8281 taggtgcgat ccttggcggc tctttatctg gtcttctttc tgataagctt ggacgacgaa
     8341 aaatgattct tctttctgca cttattttta tggctggttc tgtcctttct gcatctgccc
     8401 ctcacaatgg ttcttatttt ctgattgcag caaggattct tctaggttta gctgttggcg
     8461 ctgcatctgc cctagttcct gcttatatgt ctgaaatggc tcctgctaga ctaaggggaa
     8521 gattgtcagg gatcaatcaa gtgatgattg cttctggcat gctcctttcc tatgttgcag
     8581 attatttgct aaaagtgttg cctgaaacga tggcttggcg cgtgatgtta gggctcgctg
     8641 ctgtccccgc gctgatttta tttttcggtg ttttagctct accagagtca ccgcgttttc
     8701 ttatgcaatc tggccgcttg gaagaggcaa aaagagtatt gaactatatt cggacaccaa
     8761 aagaagctga acaggaattc gaacaaatcc agctgaatgt caaacaagaa aagacaaccg
     8821 gaacttcttg gcatactttg tttttagaaa aatatcgttc cttagtcatt gccggtattg
     8881 gtgtagcagt attccagcaa tttcaaggag ctaatgctat tttttactat attccgctga
     8941 tcgtagaaaa agcgactggg catgcagcga gcgatgcatt gatgtggccg atcatccaag
     9001 gaatcatttt agttgctggt tctttgctct ttttggttat tgcagacaag ttcaatcggc
     9061 gaactttatt gaaaataggc ggtagcgtga tgggactttc attcatccta ccagctgttt
     9121 taggaactgt actggatgct catacaaatt cgttgttgat tctcttgttt ttatgtattt
     9181 atgtcgcatt ttattcttgc acatgggctc cactgacatg ggtaatcata ggcgaaatat
     9241 tcccactagc tgtccgcggg cgagcatctg gcctagcctc ttcttttaat tggattggtt
     9301 ctttcctagt tggcttgctt tttcctgtga tgactgcttc tatgtctcaa gaaatcgttt
     9361 tcggcatttt tgggatcatc tgctttttag gggttttatt tattcaagag atcgtaccag
     9421 aaactcgtgg gaaatcttta gaggaaatcg aacaaagtgc atctaaaaaa acttatccta
     9481 aacgtatttc tatccatcat tcttaaagag gttgtgacag aagtggtcag cttcaagaaa
     9541 taagaaggaa ccgcttagtt tccagtcaca aggaacaatt tcaaaaaaca gcttctcatg
     9601 tttgatgaat attgtgactt gtgaccagtt ggtaggagaa caccgtttag tggagaggtg
     9661 gtgaaagaaa cgttcagcct tgagcgatga caaaagctct cacaaacatt gcgacgagta
     9721 accgtaggag caataagagg cggtaaaaac ccgtctctta tttttcgttt tctttcactg
     9781 tttcccgttc aactagtaca ggttcataga gaatatttgg ctgtgcctgt cctttttcaa
     9841 tcgttgtgac gatccaccgt gcggcatctt gtcccaactt ttcttttgga tgcgtcaatg
     9901 ttgtcaattt tttattccct aaatggctta aagaagaatc gtctgttcct acgatcgata
     9961 ggtcatctgg aatatgatag ccattttctg tcaacttatc tagcattaaa ctagctattt
    10021 gatcgttata gcaaatgatg ccatctaaag cttgttcttc tttcaaccgt tgtactgttt
    10081 tttcagcgat tgtttctcgt gtttccgtgg tgaaagtaat gatatcttcc gtcaaaaaac
    10141 gcacattata ctgttcacac gcgctgataa aacctttcat cctgtatttt ccttgaagat
    10201 cgtcgatttt tgtgatgaac aacagacgct gatgtccttt ttttatcaaa tattctgctg
    10261 ccataaaacc gactttcaca tcatctaaac aaatataaga ggcatttaac tcttcataag
    10321 cagcattgat catcaccatc ggaatgcctt gttctctcaa agcaacataa agtgctaagt
    10381 ttgggttata ctgattactt ttcgtaggct caacgatcaa accatctacc ccataagcaa
    10441 tcattttctt caaacactct ttctccaatt cgtgatcgtt attcgtactg gctaaaagca
    10501 atgaataccc agattctcgt aattcatttt cgattcctcg gataatcgac ggaaaaatat
    10561 aatcagagag atatgtggtg atgacaccga tcgtttttgt tctgttcaat ggtgtttttt
    10621 ctttcttata gctgtcatct acatatgtgc ccgacccttt ttctttacgc agatagccct
    10681 catttaccaa cacagcgatt gcttgccgaa tcgtatgtct gctgacgtca tatatttctt
    10741 gcagcttgaa ttctgaggga atggttgtat ttacttcgta ttcctcacga aggatctttt
    10801 gttttatctc ttctgcaata tcttgatatt ttgccatttg ccttgcccct ttttatttgt
    10861 acgtacatat atcataacga gtctgcaagg tttattcaag caaaaatcaa ggtttcttgt
    10921 gctcataagg catacggaac aggttcacca aattctggaa atccttgctc attccacgta
    10981 aagcattgcg cgttcgcgtg ccgattagga ttgtctaacg gatccccttt ttggttcttc
    11041 tctggtcgag catgatagat caaaatatca aagcacccgt cttctgaaac cgtaaacgag
    11101 ttgtgaccag gaccaaattg cttatttttt tcagaagaaa cgaatactgg ctccgataat
    11161 ttatgccatg attcttttaa taaaagattc ttgtgctcat cagcccacag caggcccatg
    11221 gcatagtttt catctgttgc actaccagaa tacgtaatga atattttccc attgcgagta
    11281 aggactgctg gaccttcatt tactgaaaaa ccccgttttt cccattcata ctcaggtata
    11341 gaaaggagga tctgcttccc ctttaacgtc caaggattat ccatttcgga tagataaaga
    11401 ttagaattcc cagtaatttc aggatcttgt tgggcccaca cataatatag ctttccttga
    11461 gaagagaaag ttgttccgtc taaactgaaa gtttcccttt tcgtttttat ttgtcccttt
    11521 tctatccaat ctgtattcat tggatctata tgctgatttt caagtacgaa catgcggtga
    11581 tgatgagatt catcacgtat agttttatga ttgcttgcag caaagtaaat ataccatttt
    11641 ccattgatat aatggacttc aggtgcccag atcaattcac tcatcggtcc ttcttcatga
    11701 gcctgccata caatcaggct tgcagcatgt tctaattcat tcagtgactt tgcccttctt
    11761 aattcaatca cttggtagcc tggaaccgat cctgtaaaat aataataacc atctgtatgt
    11821 ttatataccc aaggatctgc ccgttgccga acgataggat tattataaag tgtcttcatc
    11881 ataacaaatt tcctcacatt ttctttattc taattacatt ccaagagtat ttttctatcc
    11941 ctacatttaa ttcgtcatgt ttttctattg aaaaatagtt tatttcaggt tttatttttt
    12001 ctggttcttc acgtgtattt ttgtccaaca gatcgctact ggttaatgtg tagtgctctc
    12061 cttgatctag caaattgaaa tcatgttcaa tcgttatctt ttgttgttca tttgtgcgat
    12121 tgatcaagaa aatagtcagc tgattgccgt tatctacgat cacactgtcc acataaggaa
    12181 cattttttat cttttcacaa tcataaacag ggcaaatgat ttgttgtttc aatacttgac
    12241 ctttagcata tttcgataag tgttgcatca cataataagt tggattcctc catgcttgtc
    12301 ctttagcatc tgtcagtatt aaaggtattg tgtttactaa caatgattga caactaatct
    12361 ttacgcgatc tgcgtgtcgt aaaatagcca gccctaatga accaaataat aatgtatctt
    12421 ccatagtaaa gtaacaccaa tcgatgggac taccgacttc aaaatcccta tgtctttttt
    12481 ccggttgggc atgtacattc cattcatcaa acgctagata cattgttttc tcacttctca
    12541 aatatccttt tacataatca caagtagcaa cgatttcttc aatttgacga tcgaatcgat
    12601 aagcttttgc aaggtatgtc gctgtatcat cacgctcatt tttaggaggt ttagtcaagt
    12661 cttcttcatc atacttatca atatagttat gcaaagctaa ataatctaca ttctcataag
    12721 cttcttctaa tgcgatacga tcccattcag ggtacgatgg taaacgtggc gtagagcttc
    12781 caactaagat tgtttcaatt gaatcatcca ctaacttcat gacttttgag gcttcatttg
    12841 ctaacctacc atattcataa gcactcttag cccccacttg ccatggaccg tctagctcat
    12901 tccctaaaca ccataattta acgccaaaag gctttttttg cccatttttt attcttaaat
    12961 cactccagaa ggttcctttt ggaaaattac aatattctac taaatttttt gcttcatcaa
    13021 ttccttttgt tcctaaattc accgtgaata tcggtttcgc ttgtacatga ctgatccatt
    13081 taaaaaattc gtggagtcca aaagaatttg gttctatacc tcgccatgct aaatcaattt
    13141 tttttggtcg ttgttctttc ctacctacag tatccaccca atcataacca gatgtaaagt
    13201 ttcctccagg atagcgaatg acacctaatt ctaattcctt tacataagag aggacatctt
    13261 gtctaaaccc gtcaccatca gctgtttcat ggcccggttc ataaatccct gtatagacga
    13321 cacttcccat atgctcaaca aatgagccaa acagccgaga actgatctct cctaccgtaa
    13381 aatcctctct gatttttact ttcacttctt cgtccaacag ctttccctac ctttcatttc
    13441 ctatagtcaa gataatagat acaagtttcc atacgccacc agcaaagata aaaataaaag
    13501 gagggaaaag aaatatgagc agtacaaatc ctaataacaa tacgcaacaa agaatatttc
    13561 tcaaaaaatc aaaaaatact ttccatgcac tttgttcaaa aatttgtaaa aatgtgatat
    13621 ctttgttttc agaaatcaca tatatccagg ataaaagaaa catggaataa ataagtaaaa
    13681 ctacatatcc actaattgaa agtacgattg tctgttcttc taataattga ttaacaagaa
    13741 aataaataat gatcgttaac agactaagga acaagttctt accaaaatgt tgctttattc
    13801 cttgaaagaa aaaataaatg atatttcctg atccctcacc tttccattgc gccattacat
    13861 aaaaaacagc acatgtagca ggcacgatag taataacagg caaacacacg attacccaga
    13921 gaatacctaa taagatgtaa tcagaaacca caatcaactg tccataaaat ttagtctcaa
    13981 ctattttcat cttcctcact ctttcattcc agacatagct gttccttgaa caaaatattt
    14041 ctgtgcaaaa atataaacca ctagtaatgg cagcattgaa ataaccgttc cagccattac
    14101 aggtccataa aaagaaatgt tctgccctgt aaataaagct aaccctgctg gtaaagttct
    14161 catttttgaa ctacttgtca atatcaatgg ataaagcaag tcattccaac tattcatcag
    14221 cgtgaaaatc cctaatgata aaagagtagg tttgctcaat ggcaacatga ttttcaaaaa
    14281 aatattaaac tcacttaatc catcaatgcg tgctgcctct tccaaagaat ctggtaaagt
    14341 aataaaaaag gaacgcatca tgaagatacc aaatgcagtt gtcatacgag gaatgatcaa
    14401 acctgcataa gtatcaagca tgccaagcaa attgacttca ataaatagag gaatcatgaa
    14461 gacttggaat gggatcatca ttgtcagcaa aacgaaatag aacaaaactg attttccttt
    14521 gaaacgcata cgagcaaatg cataccccgc aagagaatca aagaatacag aagtgatcac
    14581 tgtccctcca gcaaaaatca ctgtattttt gatgtaatct aataaaggga tcgttgtcca
    14641 aaccttaata tagttatcta gggtatagct atctgcaaat attctaggcg gaaatgagat
    14701 aatgtctttt tcgtgtttaa aagaagaaat aatcagccac aaaaaaggaa acgcaataac
    14761 caacaatata actagcatac taatcgttcg aatgattttt ttaccactaa ctgcttttcg
    14821 actattcaca gtaactgtaa tcgagctttc tggtgccata caggtttccc tcctatttca
    14881 tttgtttttc tttccgtgac atgtacacat tagtggagat agctatagtt gcaacaatga
    14941 taaacaaaac cgaagacagt gctgatgcat agcccaattc ataaggtgct gtaaatcctc
    15001 gactgtaaat atattgaaca gctgtttctg tcttaaattg tgggccacca gcggttgcaa
    15061 catatacttg gtcaaacact tgcatcgaac caatcagatt cgttaaaata caaaaaccta
    15121 aggagggaac gatctgaggt agcgtaatgt taaaaaactg ctgtcgattt gttgcaccat
    15181 ccatttgagc agattcatac aaagagggag agattccctg aatagcagtc aagagaatcg
    15241 tcatagtaac accaaaattc ttccaaacag tcatgatggc taccgtcggc atagcaaggg
    15301 ttggatcgcg aaaaaattga gggatcggca agccgacttg tgaaaacaag taaggaatcg
    15361 cccctacatt cggatcaagc aacatagaaa aaataatcgc aattgacgtc aatgaacaaa
    15421 taactggaat gtaaaatgtc gaacgacaaa aacgattgaa tctggtattt ttagataatg
    15481 ctactgctgc taataaacca acgataattt gtgttggcgt ttcgagtacg gtgaaatata
    15541 aagaatgaat caacgaatta actgctcgtt catcactaaa tatacgtgtg aaattttcaa
    15601 aacttacaaa atttcttgat gtaaagaaaa tattcaaatc tgtaaaacta atagcaaatg
    15661 ttcctaaaag cggtatgaca gtaaataata ataaaactaa aaaagatgga gttaaaaaca
    15721 aatacgcagc acgatcagga cgattccaat atttggtgaa tccgttcata tagtccacct
    15781 catctttcat tttgataaaa tatacaatct tggtttaaac gaatctgaat aagatatccg
    15841 aacaatcgct gtgatttttg caataacacc tatctgcaaa tactaggtcg atcattcaga
    15901 tatctgtatc ttgccaacag tctatcctgt ataaagttct gtactatttt tgcagtaatt
    15961 gattgatggc atcatctgca tcgttcatga gtgtctcagg atcttctcct gctaataatt
    16021 tttcaatcag tggattgatg atatcgttat tgatcgcaga gatttgggta aaacctggta
    16081 aatatggctc tgcatagtcc atttgtttcg ataattcaga tacaatagga tcatttttta
    16141 cttcttcatc ttttgctact gaatccaagt aggcaggaaa accattttcg atactccatt
    16201 ttttaccaat ttcagttgta ttccaatatt tcaaaaactc atagacagct tcttttttat
    16261 tattatctgt acttgccgga atggcatatc ctacgccatc taaaatagct gtttttttgt
    16321 ctgaatcact cccttgtggt aaggtagtca cattataatc aatctcgttt gctttcaatc
    16381 cgttatttaa ccatggccca ttgatttcaa tggctagttg accagcattc attaaattgt
    16441 ccatttcggc gccagaaatc gattctggac cagtcttgtc tacatggatc attttttgga
    16501 tcgcttttaa cgttttaagg ttctcaggag acgcaaattc agacttagta taatcatcgt
    16561 tcactaattt accgccattt gctaacatcc agttatataa cggtgcagtt ccatccttat
    16621 tgaagacaaa acctgataca tttttactac tatcagttaa ttttggtgcc atttctgtca
    16681 attcttccca agtagtaggt ggttgttcag tatctaatcc agcttgttta aacaaatcat
    16741 tattccaata catatacatg ccttgtacct gcatggggac aaaatactgt ttaccttcta
    16801 cttcacctaa ttcaatagcc gtttctttaa aatcagattt atctactcca tcatattccc
    16861 agaaatcgtc taatggttgt accgctccgt tttctacata ctgtgcaaaa tctggataat
    16921 ttaatgcaat aaagtcaggt gcttttttag aagaaatcgc tgtaggaagt ttttcattca
    16981 aattggccca agtcatgata tccatttcaa ttttgatgtt tttatcattt gttttattaa
    17041 attcattaac gatttcttgc aatatttcac catcagatgc agtaaaccca ttccaaaaag
    17101 tcaaagtcac ttctttatca gaatcagatt ctgctgaact tccgccaccg caagctccta
    17161 aaatacctgt tgcagctacc aacaaagagc caattaacca ttttttacct ttcatttttt
    17221 gttcctcctt catctgataa acgacatctg accacattcc aagatagcgg tttcaataaa
    17281 caattgattt tcccatcttc agcttttaca tttgttgaat gacttggttt tacttgttca
    17341 cttttaggaa gattagttgc ttttgtatca aatccactca tttcagaaaa atcaatcact
    17401 ccctctaatg ataattcaga caagtgaaca gttagattca tttcttcttc tccacggtta
    17461 actgcaaata tgacaagttc tttgtcattt attacggcta tactttctaa atacggcact
    17521 tcttcaaaat cttccgtcgc atagtttgct gaacgaattt gtgggcttaa cacctctcct
    17581 tgtccataca cagcagcttg cataaatgga taatagatcg tctgtctcca agcttctccg
    17641 tttgtttctg tcctgattgg tgcaataacg tttaccaatt gagcaagaca agcaatcttt
    17701 actctatctg catgtttcaa taaagtgatt aatagacagc ctaatagtaa tgcatcttca
    17761 aaattatagt tatcttctag taaaggagga gcaatctgcc aaggcgctgc ttgctcatct
    17821 tgttccgctg aatgatacca aatattccat tcatcaaagg acaggttgat ctgcttctta
    17881 ctatgtttct tcgccttgac tgtatcgcac atcgctgtca cttcagaaat gaaccgatcc
    17941 atgtctaagg aacgtgcaag ataatgagaa agatcatttt tttggttgcc ataatattga
    18001 tgcagagaca aataatctat ttgatcgtac gcttgatcaa gaactgttaa ctcccaatca
    18061 ccaaatgtag gcatttgccg attcgaactt ccgcataaaa ccacctcaat tgaatcatct
    18121 acgagtttca tcgccttagc cgtttcctct gctaaccgtc catattcttc cgctgtttta
    18181 tgtccaatct gccaaggacc gtccatttca ttccctaaac accatgtctt aattccaaaa
    18241 gggctctctt gaccattcga acgccttaaa tcactccaat aagttccttt aggaaaatta
    18301 caatattcga ctaaattacg agctgcatct actccacgag tacccagatt gacagccata
    18361 ttgatttcgg cccccacatg ctgacaccag ttataaaatt catgaatccc aacttcattc
    18421 gtttcaattg tccgccaagc taaatccaac cttctaggtc tatttgctat gggcccaata
    18481 ctgtcttccc aacgaaatcc tgacacaaaa tttccaccag gatatctaat catgggaatg
    18541 ttcaattctt ttaccaagtg aatgacgtct tggcgaaatc cacttttatt agctgatgga
    18601 tgatctggct gataaatccc ttcatatact gctcgtccca tatgttcaat aaatgaactg
    18661 tacaaccgcg gatcaacttt gcctacacgc ttgacactac taatcaacat tgtggcttcc
    18721 ataactgacc tcctttaatt tatacatgaa taatacacct cgttgaaagc gttgtcaata
    18781 atataaatgt taaacttact tattttttat cgattttaat taaccaaaat attaataatg
    18841 attaaaacaa ataatttaat ataactttta ttaacaaata tgttaatata taagaaaaac
    18901 cttttaagag gtgatatata tgcaactaca aataaacgaa gaatccttac cggtatatga
    18961 agcattagct agtaaaacaa ggatcaagat catccagcta ctttccaaaa aaaagatgaa
    19021 tgtcaaagat ttggcaaaag aacttggagt gagcagtgct atcacgacga tgcacgtgaa
    19081 aaagctagag gaagcaaaca tcatcaaaac agaaaaagta ggacaacaaa aaatttcgag
    19141 ccttcgtgtc gataaaatcg atattagttt tccagaaaaa atattcaatg cttttgatac
    19201 gaaagaaaca tcgataccta ttggtcacta tacaaattat gcaattgaac caacatgcgg
    19261 tttagctacg atccatgatt ttatcgggaa agtggatgaa ccacgctatt ttatggatcc
    19321 tcgaaggatg gatgcccgca ttctctggtt taccagcgga tttgtcgagt atcaggcacc
    19381 taatttttta aatacagagg atacattgga gatgttggaa gtttctgtgg aaatttcttc
    19441 tgaatttcct ttttcaaatg acaattggcc ttcagatatc actttcagcc taaatggtgt
    19501 ggagctagga acatggacga gccctggaga ttttgctgat atccgcggta aatatacgcc
    19561 tgattggtat ccagataatc ttaatcaata cggcttgctg aagacgatcc gaattaccaa
    19621 acacttgacg aatatgaatg gagaaccctt gtcaaacatc acgatcaacg acatccctaa
    19681 agagcaagac acttggcatt tgcgaatcga agtgaaagac gatgccaaac atgtgggggg
    19741 ctgtacttta tttggtaaag gattcggtaa ttacgatcag gatatcaaat taaaagttta
    19801 ctacagctga tatttttctt ttctatttta taaaacaaaa acagccaatc cctatgaagg
    19861 gacggctgtt tttgctttat tgttatttcc agaaatcatc caaaacggaa attggcaaat
    19921 gtcgtttatg ttgtgtcttg ttccaccatg cttcgatttt ttcttgtgct tgagcgggaa
    19981 cttcttttcc ttctagataa tcatcaattt cattatatgt gacacctagt gcgacttcat
    20041 ctgcaatcat tggtttccca tcttcaagat ctgcagtcgg aatttttgtg tataattttt
    20101 ccggtgcatt caatgcttga agcaattgtt tcccttgtcg tttgtccaaa cggaataatg
    20161 gcaaaatgtc ggctccacca tcaccaaatt tcgtgaagaa tccagtaatg ttttctgcag
    20221 catgatctgt ccctaatacg gctcctgctc tttctccagc tacggcatat tgcgtaatca
    20281 tacgttgacg tgctttgata tttcctttgt taaaatcgct gattcccaca cctgcttcgc
    20341 ttaatacttg tacttgtgca tcaacagctg gtttgatatt tactcgcaga ctaacatcgg
    20401 gctggataaa gtcaagagca gcttttgcat cttcttcatc tgcttgttca ccataaggaa
    20461 gacgaacagc gataaattga tagttttcat cttttgtttc ttcacgcatt tcttccatcg
    20521 ttagttgcgc caaacggcct gctaaagtag aatcttggcc gccgctgata cctagtacaa
    20581 aagttttaag aaacggatat tttttcaaat atgctttcat aaagtcaata cttttacgta
    20641 tttcttcttt tggatcaatt gttggtttga cacctaattc attgataatt tctgtctgca
    20701 ggctcattta ttctcctcct atgcttctgt atgaagttct gctacgcttt ttttcattcg
    20761 ttccatgata gccattttat ggttccagca ttcagtagac agatcgactg ggtatttttg
    20821 cggattcaag tctcgtttat actcttccca tagtgaatct agattttctt ttgtatattc
    20881 tttgatttcg tttagtgcag gtaattcgta aactcttttt ccttcgataa agatatcttg
    20941 taaaactggg cgagctgtaa agtctcgaac agttttattg ataaacgtat gcactggatg
    21001 gaacatgaag atagcttctt cttctcgagg gtcttcatcc cataatgtca cgtagtctcc
    21061 ttcagatttc ccatcaaaat ttcgagtgat gcgccacact tgttttttcc ctggagtagt
    21121 tactttttct gcattgcttg ataacttgat cgtatccatc atttttcctt catcatcttc
    21181 gatcgatact agcttgaata ctgcaccaag ggctggctga tcataggctg tgatcaattt
    21241 tgtcccaaca ccccatacat caattttagc tttttgcatc tttaaattca aaatcgttgc
    21301 ttcatccaag tcattcgaag catagatttt tgcttccgta aatccggctg catctaattg
    21361 ttcccgaaca cgcttagata tataggccat atctccactg tcgatgcgta cgccttggaa
    21421 gttgattttg tcacctaatt ctttcgccac gcgaatcgca cttggcacac ctgatttcaa
    21481 cgtatcataa gtgtcgacta agaacacgca gtctttatgg gttttagcgt aggccataaa
    21541 tgcttcataa tcatttccat aagattggac taacgaatga gcatgggtcc cgctagctgg
    21601 tatcccaaat atcttcccag cacgtacatt gcttgttgca tctgctccgc cgatatatgc
    21661 cgctctagtt ccccagactg ctgcatccag ttcttgtgcc cgtctagttc caaattccaa
    21721 tagtggatct tctccgatga cagatttgat acgagctgct tttgtcgcga tcaacgtttg
    21781 gaaattgacc atattcaaca gtgctgtttc tactaattgg cagtgcgcta aaggcccttc
    21841 cacttggatc aatggttcat ttgcaaaaac tagttctcct tcacgagcag aacgaactgt
    21901 tgctttaaat tcaaaatttt gtaaatactc caaaaaacct tcaggataga cttctaatcc
    21961 acgcaagtaa gcaatatcgg aatcattgaa cgtcagattc tctaaataat tgactaaccg
    22021 ttccaatcct gcaaaaaccg cgtatccgtg gttaaaaggc atttctcgaa agtaacattc
    22081 aaaaacagca tgtaaatcag ctcgacctaa ttcccagtat gtctgcatca tattgatttg
    22141 atatagatcc gtatgcagcg tcaaactgtc gtctgggtaa gtattattca tcttgtatta
    22201 ctcctcttgg caagttttca tttcctaccc attataacaa aaaaaattaa taggaaaaga
    22261 aatcttccat tttttctgaa aaagcacatg ttttacggat atttcttgct aaaaatgaac
    22321 tccatctgct aaaactatcc tttcttattg gtatttaacc taaacgagaa agaatactta
    22381 ttttattcaa tgtatccgag acacgaagaa attagcgttt tctatttttc ttacttgata
    22441 aaataagtag attttcctac tacaatcgta gccgttaaga taccgatgat tgaacaaata
    22501 taaccaaatg tctctacaac ataatcaatg gcaaaagttt ttgaagcaat aatacgtctt
    22561 tccttttttg cttcttatcc ccaaagaaac ggatataact tattatgtat tgaatttttt
    22621 tggaaaggcg aattatgaca agatatacct tgcgttgaaa aaactcgcgg aaaaaatatc
    22681 acacgttttt aaaaagatac aaagtaagaa agcagctgtc aatagaatgg aaacaaaaat
    22741 ggcgagacat caatcagtct cgccttgttt tt
//
LOCUS       DARBFO010000036        22510 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000036 DARBFO010000000
VERSION     DARBFO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.037"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..136)
                     /locus_tag="SCK51_001510"
     CDS             complement(<1..136)
                     /locus_tag="SCK51_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252146.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     E"
     gene            complement(227..679)
                     /locus_tag="SCK51_001511"
     CDS             complement(227..679)
                     /locus_tag="SCK51_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEG1252147.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(692..1102)
                     /locus_tag="SCK51_001512"
     CDS             complement(692..1102)
                     /locus_tag="SCK51_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1252148.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1377..1589
                     /locus_tag="SCK51_001513"
     CDS             1377..1589
                     /locus_tag="SCK51_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1252149.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1592..1804
                     /locus_tag="SCK51_001514"
     CDS             1592..1804
                     /locus_tag="SCK51_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252150.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            1944..2099
                     /locus_tag="SCK51_001515"
     CDS             1944..2099
                     /locus_tag="SCK51_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252151.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2096..2320)
                     /locus_tag="SCK51_001516"
     CDS             complement(2096..2320)
                     /locus_tag="SCK51_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEG1252152.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2398..2586
                     /locus_tag="SCK51_001517"
     CDS             2398..2586
                     /locus_tag="SCK51_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252153.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2912..3247
                     /locus_tag="SCK51_001518"
     CDS             2912..3247
                     /locus_tag="SCK51_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252154.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3480..4421
                     /locus_tag="SCK51_001519"
     CDS             3480..4421
                     /locus_tag="SCK51_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HEG1252155.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4423..5313
                     /locus_tag="SCK51_001520"
     CDS             4423..5313
                     /locus_tag="SCK51_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HEG1252156.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5356..6156
                     /locus_tag="SCK51_001521"
     CDS             5356..6156
                     /locus_tag="SCK51_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEG1252157.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6171..7022
                     /locus_tag="SCK51_001522"
     CDS             6171..7022
                     /locus_tag="SCK51_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1252158.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7061..7222
                     /locus_tag="SCK51_001523"
     CDS             7061..7222
                     /locus_tag="SCK51_001523"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252159.1"
                     /translation="MLQIIGDRDMDELSQQESRMVRKISKLIIYRDTERFLTVLGNVY
                     ENPELLEVE"
     gene            7223..7684
                     /locus_tag="SCK51_001524"
     CDS             7223..7684
                     /locus_tag="SCK51_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1252160.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7710..8162
                     /locus_tag="SCK51_001525"
     CDS             7710..8162
                     /locus_tag="SCK51_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252161.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8268..8462
                     /locus_tag="SCK51_001526"
     CDS             8268..8462
                     /locus_tag="SCK51_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252162.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8493..8786
                     /locus_tag="SCK51_001527"
     CDS             8493..8786
                     /locus_tag="SCK51_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252163.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8816..9256
                     /locus_tag="SCK51_001528"
     CDS             8816..9256
                     /locus_tag="SCK51_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1252164.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            9331..9798
                     /locus_tag="SCK51_001529"
     CDS             9331..9798
                     /locus_tag="SCK51_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEG1252165.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            10545..10775
                     /locus_tag="SCK51_001530"
     CDS             10545..10775
                     /locus_tag="SCK51_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252166.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            10934..11767
                     /locus_tag="SCK51_001531"
     CDS             10934..11767
                     /locus_tag="SCK51_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HEG1252167.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11760..13175
                     /locus_tag="SCK51_001532"
     CDS             11760..13175
                     /locus_tag="SCK51_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG1252168.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13187..14716
                     /locus_tag="SCK51_001533"
     CDS             13187..14716
                     /locus_tag="SCK51_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG1252169.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14634..15686
                     /locus_tag="SCK51_001534"
     CDS             14634..15686
                     /locus_tag="SCK51_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEG1252170.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15688..16005
                     /locus_tag="SCK51_001535"
     CDS             15688..16005
                     /locus_tag="SCK51_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252171.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16132..16755
                     /locus_tag="SCK51_001536"
     CDS             16132..16755
                     /locus_tag="SCK51_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEG1252172.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16770..17660
                     /locus_tag="SCK51_001537"
     CDS             16770..17660
                     /locus_tag="SCK51_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HEG1252173.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17683..17898
                     /locus_tag="SCK51_001538"
     CDS             17683..17898
                     /locus_tag="SCK51_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252174.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            17910..18251
                     /locus_tag="SCK51_001539"
     CDS             17910..18251
                     /locus_tag="SCK51_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252175.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18251..18622
                     /locus_tag="SCK51_001540"
     CDS             18251..18622
                     /locus_tag="SCK51_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252176.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18615..19010
                     /locus_tag="SCK51_001541"
     CDS             18615..19010
                     /locus_tag="SCK51_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEG1252177.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19012..19389
                     /locus_tag="SCK51_001542"
     CDS             19012..19389
                     /locus_tag="SCK51_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252178.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19390..19998
                     /locus_tag="SCK51_001543"
     CDS             19390..19998
                     /locus_tag="SCK51_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1252179.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            19998..20339
                     /locus_tag="SCK51_001544"
     CDS             19998..20339
                     /locus_tag="SCK51_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252180.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20554..>22510
                     /locus_tag="SCK51_001545"
     CDS             20554..>22510
                     /locus_tag="SCK51_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEG1252181.1"
                     /translation="MNNDDLILKMILDESGFTAGMNNAVKKLNSFDETIERKSRNSGS
                     SLGSIWKIFAGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNS
                     EIMKNIGKNGLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFG
                     GSTDQVNEAVIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLS
                     QGKISVEEFQNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIE
                     GLNKALVDSDLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALK
                     FMMPFLVPAATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVL
                     IIGAVVGAIAVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEM
                     ASNAVEGVKDAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKW
                     FSDTWQSIKDSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKN
                     VFEILKNVILAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFT
                     NIYFAALNIWTGFKLTLINIWNEVVNQA"
BASE COUNT         8074 a         3306 c         4618 g         6512 t
ORIGIN      
        1 tttctctatc atgtaattta tttagttttt catcattttt ttgttctaat tcataagctt
       61 ctttttttat tgtgttagct ttcgacagtt tagtgattcc tataattaat aaaataagtc
      121 caatcaataa taacattcaa aactcctcat ttctttgata taattattgt agagtttact
      181 cagaaatgag gaaagagtcc atgtgaaagc atgggctttt ttatttttaa tgttgtaata
      241 ctaaatgttc aagattatga ggaagcccta aatattcaat aatttggaac tgactcatat
      301 cagataaatc ttgttcatct attgttctag ttaacatttc aattgcaaac atattagctt
      361 cagcttctat accataaatc atacaactaa ggttgtttgc tctaaaagct gctgtgcaaa
      421 tacctttatg gagtctaacg tgggctattt catgccataa tacaaatctt ttcatagaag
      481 gtgataggtt ttgattaatt aatatcacat aacatctttt attgtgtatc gtttgtccta
      541 atgttttatt ccctaaatca gtttcaatta aatctatatc agaatatttg attattttat
      601 atggattaat cgcatcagat ccaaactcta caaataattt ttctatttct tctaatatcc
      661 agtatttgtt taaatgcata ccaaagccca cctattgacg atatttttta ggagtgaatt
      721 ttttctttgc tttaactttg gttaaggcaa gtgcaagttg aatggcatcg tataattttt
      781 ccttatcttc atcgtctaaa ggttcaccat aataatttgt ttcagcatct gaattcaagc
      841 cttcaagtaa tttttcagct tgtaaagcaa tatcttgttt atctttttct gttaaatcat
      901 aataatggcg tttatcagta cgtcctaaaa gataatcagt tgatacatta aagtaatctg
      961 caactttttg tattttatct ccagatggag tacttttatt ccatctagac atgcttccat
     1021 ttccgaaatt taactttctt tcaagttctg ccaacgtcat tgaacgttct tttaataaga
     1081 tttttatacg gtctaatagc ataggatatg cattcctttc aaaataccga caaaaaaagt
     1141 atttttctgc aaattatcta tttacaaata gagaatttgc agttatacta gttttgtaag
     1201 caagatattt acggcataca aagactaaaa gcaataaact aatagtctcc ccagacatca
     1261 gtaagctcgc tttttgtgcg ctgtattttg tatgcttaca taatagcata tttgcagttt
     1321 ttgtcaaact tattagacaa aaatattaga aaatattcga taagggggta tgaagcatga
     1381 ctttaaaaca gcaagtatta attgctttag taaagaaagg ttggagtcaa agagagttag
     1441 ctcgacgaat gggtatatca attacttatt tacgagatat cttcatcgaa aaaagaaaac
     1501 ctaaggaacg tttaaaacaa atcgaagaac ttttggatat caagttagaa gtagattcta
     1561 atgatcagcc atcagaacag gaggcgtaaa aatggtacaa cgtttgagca aagcggagtt
     1621 taaagagaaa tataagtatt ctgaaagtac ttatcaaaga agaatgaagg aatttaaaaa
     1681 atcacgtttt agtgaggggt ataacgcagt tacttccaaa gaaataacaa ttgatgtcga
     1741 tttatatgaa cagtttagaa catggcgttc taaaaatcga tttagagtta gagctgtttg
     1801 ttaatagcta aaaaatctaa attcaccact tctgaaaaga ggagttgctg ctctcctttc
     1861 aagtttgcca aagtccacgt tttttcattt tggcaactcc tcttcttgga ggtggtgacg
     1921 gcgagagacg aaggagggat gacatgaagg aagaatcaaa aatagggatt ttgaatgaga
     1981 acaattacac aagattaatt attgaaacag aagaagggaa aaaagtagca gaagttacat
     2041 tgaccgatgc aactcctgct gacggttatc gtattcgatt aactcctaaa tatgattaac
     2101 cttttggtgg gtgtggatcg tttccgtgac tatcacggct ttgaattttt ccatctctgc
     2161 catgaataaa tagctcagat ccttgatttt tagaaatctc tctagcgatt tttactgctt
     2221 ctgattttgt attagtatgt gcagtcgctt tagaatttcc agcacctttg acattccaac
     2281 caccattgga attaggtaca acatgttgat tttttcccat agttttcacc tccatttcta
     2341 tagaaatatt ttatcaaata gaaatggaga aattcaataa acgaaaggag aagtgatatg
     2401 gcatatacct tgcaacaaga acatcaaatt ctcggtttga ttaaacaacg caggaaacaa
     2461 ttacaagatg accgtgcagc gcttagaaaa gccgatgagc tatcagatag gcaagctgaa
     2521 ctaattgctt ctgaacttga ggatttgaga atgctagaaa taaaaaatag ggagattaga
     2581 ttatgaagaa gacagacaca ctttttatag gattcatttt ggggttatta gtgattgtag
     2641 cgcaccaaag tattatcggg ggaagcttgt tcgcagcatt gatggtttta atcaatctgc
     2701 ttgattcaaa agaaaggagc aactatggca cgagaagaag cgctaaaaat cggtaaagtg
     2761 attgctgata atcggtgggg aaatagccgc cctattattt taagcaagca acatatcgaa
     2821 aagcaaaaag catggcaaca aataaaaaag tgactctgcc agccagcaaa gagtcacata
     2881 caaatttata aaactaggag aattatatca catgaataag aaaattgaaa atcttatcga
     2941 agaattgaaa cgtgaatgcc aaaagcaagg tgtttctatt atttgtacag cccaaaaaga
     3001 aggggaactt aaaagtctcg tttatggtga gacaacagaa attttacttt gtcttgctat
     3061 gcaagaagaa catttagatg aaaatctccc attgtcagca catattatgc gtagaattgc
     3121 tgtggatgct tacgaacaag cgaaaaatga agaagaaaat caaccttcaa atcatacttt
     3181 tgttattaat aacaaggaag atttagctga tgtgatgact cgtattctca agggggaatt
     3241 tcaatgatga atgaaaaaac aattgatcta ctcattattt tatttttcac gttagtgatt
     3301 gtagcgaaaa gttatgtaat ctttggcttg ttattcgcaa ttaccatgct tgtgctttgc
     3361 gtgttagcaa gcagaaaaga ggttaaacat gaacgagtta gcaaatcttg acaactattt
     3421 aactgatcct gattatgcta aaccaccata tgaagcacca attgatgagg aggatgaaga
     3481 tgagtaaatc taccttagaa atgagccatc aagaatggct tgaagaccgt aagaaaggca
     3541 tcggaggttc ggatgttgga acaattttag gattgaataa atggaaatct ccttatcaac
     3601 tatggctaga aaaaacagga caagtagtac ttgaagaatc aggaagtgag cccgcttatt
     3661 ggggtaatgt tttagaagaa gtggttgcta aagagtttca ggaacgtaca ggcaaaaaag
     3721 tacgcagaag aaaccaagta tttgaacatc cattacatcc atttttaaga gcaaatattg
     3781 atcgggaagt agtgggagaa aatgccattc tggaatgcaa aacagccaat caatttctcg
     3841 gtaaagaatg ggaaggcgaa gaggtaccgc ttagctatct ctgtcaagtt caacattaca
     3901 tgaacgttct aaacaaagac tattgttaca tcgctgtctt aatcggtggt caaaaattta
     3961 tctggaagcg gattgaacga gatcaagagc tgatcgatac aatcactgaa caattagtag
     4021 agttttggga aacaaacgtt cttggaggta tcgagccaat tattgatggt agtcaagcga
     4081 cagctgattt cttgaaagag aagtatgcag atgttgaaga cgttcaaacg acgttaccaa
     4141 ttcatttcga tgaactagtt gaacagaaaa acgaactcaa acggactaag aaagaaattg
     4201 aatcagctat ccgtcaagta gacaacgaga ttatcagcga gctaggtaaa cgtgaagcca
     4261 gtatcggtat cactcaaaaa aacatcatca gttggaaatt tgtccgtacg agacgtatga
     4321 actcgaagaa actagcagag aaatatccag atgtcgcaaa tgatgaagag atttataacg
     4381 ttactgaatc aagaaggcta accgaaaggg agatcaaata atatggcaac aaatgaatcg
     4441 ttaaaaaatc aattggcagc aaagccacag aaacaagttg caccaggtca attaggactt
     4501 aaagctttga tgaatacacc aacaatgaga aagaagttcg aagaggtgct tcatgacaat
     4561 gctaatgctt ttatgtcgaa tgttatgact cttgtatcta atgacagtta tcttgcagat
     4621 agtgaaccga tgtctatcat gagtggtgcg ttaactgctg caacattaaa tcttgggcta
     4681 gataagaatt taggttatgc atatttagtt ccattcaata gtaaaaacaa gcaaacagga
     4741 aaatgggaaa agaaagctca attcatgctt ggctataaag gatatatcca attagcccaa
     4801 cgatcaggta aatacaaagc attaaatgtg atcgaagttt acgaaggaga actaaaaagc
     4861 tggaaccgac tgacagaaga gtttgagttt gatccaaatg gtagaacgtc tgatgaagtc
     4921 attggatatg ttggctattt cgagttactg aatggattca aaaaaactgt ctattggacc
     4981 aaacaagaaa tcgaagctca tcggattgct aacaataaag atcgcgataa gacaaagtta
     5041 agtggtgtgt gggcatctga ttacaatgca atggcacgaa aaactgtttt gagaaatctt
     5101 ctttctaaat ggggaatctt gtccatcgaa atgcaagaag ccaccacatc ggatgagaga
     5161 gtccaaaggg ttcaagaaga cggcagcatt attgctgaaa cagaagttga agaagatatt
     5221 cctgaaagaa aagaagcaga ggttatatct gaagaaaatg aagatgtaca aactggatta
     5281 tttgatgcat ctaatccgcc gttaaacaaa taatgaggga gctttctccc ttgcttttct
     5341 agaggagaaa tacgaatgaa cacaggatat gtcaaattac atcgaaaagt gatgaattca
     5401 ttcgtttgga ccaatcctta tatgtataaa ttatggaact tgtgtttgat gaaagcgagt
     5461 catgagaatc gcaagatact ttttaatgga aaagagatac aagtgaacag cggagaattc
     5521 gtcacagggc gcgatgctat cacatctgag atgaacgaag gcgtgaaacg tgaacatcaa
     5581 gtgaacagcg gttctgtatg gagatggcta aaacaatttg aaaaaaacgg aatgttgaac
     5641 atcaaatcaa ctacgaaata cagcgttata tcaataaaaa actggtcttt gtaccaaagc
     5701 agtgaacaac aaatgaacat caaacgcaca acaagtgaac aacaagtgca cacaaacaag
     5761 aatgaaaaga attataagaa tgaaaagaat aataaaacat catcgtcgca accacgcaaa
     5821 aagcgtgttt acgacaccga ctcagtttac tacattctcg cggaggagtt attcaaacag
     5881 atttgtcaga atcaggaaat caaaaagccg aatctgcaaa gttgggcaga tgacattcgg
     5941 aaaatgatcg agatcgacaa gcggaccgaa aaccaggtac ggggaatgat tgaatggagt
     6001 cagcacaatg tgttttgggc atcgaatatt ttatctgcta aaaaattgcg agaaaaatac
     6061 gacacaatgg cagcgcaagc aaatcgtgat tataaaacaa aacaaactaa aacgcttgaa
     6121 tacgagaaat ttagcacaga tgagttgcct atttgagagg aggcgtaggc atggaaactg
     6181 ttggggaaat catggaaaag ctgatacaga aagtgcttgt ccaacgtggc gaatgtcctg
     6241 aatgcggaca gcctttgtat ggatggcgta cgaagaaccc tgatggctca gaacgttgta
     6301 agccaacctg tatgcaatgt gggtataaag cattgcgagt tcaggaagac ttacaaacag
     6361 aacgaattta caacgagagc ctgaaagcaa gagcaatcaa ttttttcaaa ggtggttctg
     6421 ttgtgcctaa tcaagcgttg tttgattgca cattgcagaa ttatcaaatt gtcgatcaag
     6481 aaacaagaca agcggttgaa gtaaccaaac gctttgttaa ttcagtcttg ttaggaaatc
     6541 caagtcacct tgttttaact ggaaaacaag gaacaggtaa aagtcaccta gcgatggcag
     6601 cggcttggga agtcttgaag cgatcaaact acgataaaaa aatcttattt atcgggttac
     6661 aggaaatgct ggatcaaatc aaattttcgt acaacaatcc tgaactcaga aaaacgattg
     6721 agggatcgtt gattgcagat atcaaaacag cggatttagt catcattgat gatatcggtt
     6781 cagaactagg aaaagatgca tcagatagtc gagcgtttgg cataaacacg ctaaattcgt
     6841 tcttggatgc acgacagaac ctagcaacga ttatcacaac gaacttgctt ggtgaagaac
     6901 tgaaaaaagc ttacggtacg agaacaatat caagaatgtt tgtcaactct gatggattta
     6961 cgatggtatt ttctcaaaca gcagacaagc gcataaaacc agtcaaagga gcgatcgcat
     7021 gaatttgact aaacaagaaa aaagaatctg tcgtcagata atgttgcaaa ttataggtga
     7081 tcgtgacatg gacgaattat ctcaacaaga gagtcgaatg gttcggaaga taagtaagct
     7141 aattatttat cgggatacag aaagatttct cactgtttta ggtaacgtct acgagaatcc
     7201 agagttattg gaggtagagt aattgccaaa aatattagat gcatgttgcg gtagtagatt
     7261 gttctggttt gataagaaca attctgacgt aacatttatg gattgtcgtc agcagtatga
     7321 agaattatca acaggtcatg tgatcaatgt tgatccagat gtggtcgcag actttcgcga
     7381 tatgcctttt gaagatgatg tgttcgatat ggttgtattt gacccgccac acttaagaca
     7441 tgtcggcgaa aactcatggc ttgccaaaaa atatggaaaa ttagacgaac tttggccaga
     7501 agacatcagg caaggttttg cagagtgtat gcgagtttta cgaccttcag gaacattaat
     7561 ttttaagtgg aacgaggaac agataccact ttctgatgtg ttagaggcta ttggagaaca
     7621 gccattgttt ggtaataaac gaagcaaaac acattggctt gtgtttatga aaaaattcag
     7681 ctaacggagg aaacaaagga ggaaaagtaa tgaagattgt attaaataaa tgttttggtg
     7741 gctttgggtt gagccatgaa gcaaaaatgg aaatttttaa aagaaaaaat attgaagtat
     7801 tcccatacat caataacttt agctatgact cagatgatga atacacgaga tacacaggcc
     7861 aaaagctagg ttcgatggat tttatctact atttcaaaaa agacccgaaa atcgacaaag
     7921 ttacaggaat ttatagcgaa attgaccgac tgtacggaat tgctgatgat tccagttttt
     7981 caagtgattc aaacagaggt gataaggacc tcgttgctgt tgtcgaaaaa ctaggaagtg
     8041 aggccagtgg accatatgca agtcttaaag tcgttgatat acccgatggg gctgagtggg
     8101 aaataagtga ttatgacggc gttgaaactg cacattatgg ttttcaaaca ggtagtattt
     8161 agtccactaa tgacaactaa agaggaggca gaaggatgag tgaagatgaa tactttgaac
     8221 agtagtctat taacgacaga gatttttaac tggaaagggg tgtagtcatg gacgcatcgg
     8281 aagtagtaaa taaaattaat agtctcaaag aggttttcgg agacgtcgaa gtagtgataa
     8341 acgcacaagg ctatgacaac gaatttttta agcagattac cgacattagc attcagcaag
     8401 gaatggaaga cgaagacgga aactttatcg atgaagtagc cattttagtg acttgcgagt
     8461 aagtaattga cagctaaaga ggaggaagcg gaatgaaact aaaagacgga ttttactcca
     8521 gcggccacgg catcggcggt ttaatgctag atatgccgac aaagaatcct aaaacacgta
     8581 agaaaccaaa attcaaagtc ggtgacatgg ttcgctgcga agcagaagga ttcatctatc
     8641 catttcgtgg atatgtagaa cacgtctata atcactcagc aatcattcgc attgaaaaca
     8701 cgatggaatg tgataagtgg ttagcgaaaa gcaaagagaa tttagcagtg gtgagattgg
     8761 tggacatgga agttataaaa tcttaatttt gcttttttac aactatccta caacaatgag
     8821 cgttattcca caactggaag gagaagaggc aattgtgaac attttagaga atatagatat
     8881 taagcaaaca aggaaaaatg ctagacgatt actaaaaaga tacagaaatt tagaacgttt
     8941 ggtcggacca gtgaaaatag acttttccgt gatgactgtt accaaaaatt tgaaattcac
     9001 aattgacagt caaaacgaag aaatcattga agcgataagt actcgagatt cggttatcga
     9061 agcattaaca cggctaagta gaatccattt ccaggtactt tattatagtt attgttttcc
     9121 taataagatg tcaatgtatc aaataggaga aaaattagga tattccgata gaactatcga
     9181 gagaatgaag gcagtagctt tggttgaatt tgctgaggcg tataaatcag gagaactcat
     9241 ttcacgtaca aaataaaaaa agtcggattc ctccgactat gaataatatt tccgacataa
     9301 gtattatatc ataattgggg gaatcagagg atggtacttt tcgatgtaaa gaaatatgag
     9361 acaccaagcg cgaaagatgt tgatatggaa cgcacaaaac ataatgtcgc tgtgtttctt
     9421 tcagcatatc tatcagctag atgtagagta gggcaacatc gagaaccaaa agtgacagca
     9481 tcttattcct tggttccacc ttctacagct gatcatatat ttgaagcaga aagaatgctg
     9541 atcgataaag aagaagcaca ggaagaattt gagtatctgc ataaattgtt tgttcgaggc
     9601 tattcagcga tacagcatcc gcataagcct gatgtgactg aaaggcgcaa gaagatattc
     9661 tatgatcgtt atatcaatgg tctgcccatt tatgtaactg ctcaaaggaa taatactagc
     9721 gaagaatcgg ttaaagtaga atcaaacaga attatcatcc aatttgcttc atcgttagaa
     9781 ctggttgctt tcaagtagcc agtttttaca ctttttatac ccttttatta ccaatttggt
     9841 ttccatttta tacctttttt ataccaatca cttacctatt caacgttgta ttatgatagt
     9901 gtcgaaaaat taggaaacag gatcgacaaa ataaaatgta agggaggaaa tctccctcat
     9961 cgtttaatta agcttcgata ggcagcaacg gaaatattaa gaataaggat gtgaatttca
    10021 actccttcta aattgttctt attatctatc atccgttgct gtctattgtt tttttaatta
    10081 ttcacacgat aactaaaggt aagtgaagag aaatgattcc attaataatt tcaatttttg
    10141 cgctttgtct taatgtatat atgattggat ttaaaaatgg gcaaaataaa aaatagtagc
    10201 agccaagaat aattttatag tgtcactgtg gcggaaaggg tagacgctaa gcatgtgtgc
    10261 taggtcaatg cttcggcaac catgcaaggt tcgattcctt gccagtgact ttggcaaccg
    10321 aggcatcggc ggtttaaaaa tataggggtg cgcaatttcg tacgcgtttt gtgcatcgtg
    10381 taggttgcta ttacataact ggttaggtta gattgagatt tgggattcgg tacaaatgaa
    10441 tcgtcaaatg tctcaagcac aggatcggaa acgtccctgc ctgtgcatta catattagat
    10501 cactctttga gtggtctttt tctttacata aaggaggcta cataatgaga aactactggt
    10561 atgtatcgct aactaatgaa tatcctcgaa ccattgatga ttgttcagtg cgtgttgtgc
    10621 gttctgtaca aatcaaaggg aagtactcta ttgtcgaaat ggggagagaa gcagaaccat
    10681 gtgagattga tgcgtgcaag ctcgtttatt gcggtcatgg tttctatgat gaaccaaaca
    10741 ttcaaaataa tattaacaag aatttgaggg attagaatgc aagaaatggc ttaaaaatct
    10801 ccaatcagga caaatcaagt tagacaaagt ttccgattta aagatattga ttgaagctga
    10861 tttaatgcta aaagatattg aaaattagaa aacaaaactc aacctaaaca gaatgtgagg
    10921 tggtgtgtat tgaatggcaa gacaacgtga tccaagacgt gatgaagcca aaagaatttg
    10981 gttagaatcc aacggagaaa agcagttaaa ggaaattgca tctgaattaa atgtttcaga
    11041 ttctcaggtt agaaaatgga aatcgcaaga caaatggagc gctgaattga ttagtaacgt
    11101 taccaatggc aaaagtaacg ttactaatca aggtggcgct cctattggta atcaaaatgc
    11161 taaaggtaac aaaggaaata gccgagcttc tccgccagtg ggtaataaaa acgctttgaa
    11221 aacaggtgag tatgaaacaa tattttttga cacgttaacc gataatgaga aggacatcta
    11281 ttctagtttg aatgatgatc cttcttttgc tttgtccgaa gaaatacgac ttcttaaaat
    11341 aagacaactt cgaatgatga aaagaatcca acaagctgaa gctggactaa atgatgaaga
    11401 agtcgaacga ttgcagcagc taagaaagat taaaactccg attgaaaaaa atggtaaaaa
    11461 gctagaaatc aagcgtgagg ttatgcaaga tgtgcagatt agcagaaaaa aacatcgcaa
    11521 aattgatgat attctttcaa ttgaagattc attgactcgg attagcaacc agttagctaa
    11581 agccatcaag caaatgaatg aactttatat gaatgaatac agaactgatt taattaaagc
    11641 tcagactgat aagatccaag ctgagacaaa tgaaattggc ggaaataatt caggtgaaga
    11701 aatagaagaa tggaaacagg cagttttaaa tgccgcaaac aaacgggcgg tgaaagaaaa
    11761 tgaataatga atttattcct tttgctgata ttggttctgc cattgattat tactatgata
    11821 aaccagtagc tttttgccaa gatattttgc atttgaatcc tgatgaatgg caagaaaatg
    11881 ttttaaatga tttagctgaa ttttcaaagg tttctgttcg ttctggtcaa ggagttggaa
    11941 aaacagcatt agaagcagga gcaatacttt ggttcttaac gtgtcgaccc tacgctaaag
    12001 taatagcaac agctccgaca atgaagcaac tttacgatgt actttgggca gaggtagcta
    12061 aatggttaaa tgatagcttg atcaaaaact tactgaagtg gacaaagacc aaaatttata
    12121 tggttggtga ttcagagcgt tggtttgcta cggctagaac agcgactaaa ccagaaagta
    12181 tgcaaggttt tcacgaggac catatgttga ttgtggtaga tgaagcttct ggtgtgtctg
    12241 atccaattat ggaagctatt cttggtacgc tatcaggttt tgataataag ctgttgatgt
    12301 gtggaaaccc caataatatt gaaggtgtct tttatgattc ccataattca gaccgtgata
    12361 aatacagagt tcataaagta tcaagctatg atagtaaacg tacaaacaaa gacaatatag
    12421 aaatgattct taaaaaatat ggaaaagaaa gtgatgttgc tcgtgtccgt atttttggag
    12481 aatttccgaa aggtgcgttg gattcattta tcagtcttga aacagttgaa ttggctacag
    12541 aaaaacaaat tagtgattct ttagtcaata aaacaaaggt tgctcatatt ggtgtcgacg
    12601 tagctcgata tggtgatgat tctacgattc tctttcctag aattgctacc agggcattgg
    12661 agtatgagaa gtattcaaaa cgtagcacca tggaaacaac aggatatgtc atcaacatgg
    12721 ccaagaatct aatgagtcaa tatccgagta ttgataaagt gatgattaaa gtcgatgaca
    12781 ctggtgtcgg aggtggtgta accgaccgcc tagaagaact tatagaagac aaacattatc
    12841 cttttgaggt gtttggagtg aataacggtt caacatcaga agacgatttt tacgataatt
    12901 taggtactca actatgggga aacatcaagg aaatgttaga agaaaatatg acagcaaatc
    12961 ttaacggaga acagcctgtt attgaattgc cttctgatag ttcgttaatc aaagaattaa
    13021 gtactcgcaa attcaaaatg acaagtagaa gtcgtatacg tttagaaagt aaagatgata
    13081 tgaaaaagcg aaatattggt agtcccgata ttgctgacgc actggcttta gcgttttatg
    13141 agccaccaag tcactatcaa tttattcaat tttaggaggt gagcttttga ttagtacagt
    13201 attatcttta gagagatata agaagctacg tgttaaatat gcaacacaaa ttgaagatgg
    13261 aatgtttgat ccgaatggtt ttatagaaga tatgaaacca ttttttgctg atcgcgaaag
    13321 aaaatatcta gcttatacta gcgaaaagaa tgaaatagat aatagaccaa agcctaacac
    13381 tgatattgta aaagttaata ataaacttca tgctggtatg tattcaattg tcgtagacca
    13441 agcagtcaat cattttactg gtatacctat caaatgggat tacgatgtat cggaacaaaa
    13501 aagaacactc attcaaagat taaaagataa attcttaaaa aatgatatag aacttccgac
    13561 agttccagaa gcttttaaca aattaacaag caatcttgat tccatgagat tcgcaatgct
    13621 tgattctgaa actgcaactt ttcaaggagc ttgtggagta gctttccgat tgctagagcc
    13681 tgtggaagaa gatgatggtt ggaagttaag agcaagtaat attgaacctt ggagagcaga
    13741 aagatatgga aatgccggaa tctatatcaa agaaaagtat gactcttacc agaaaaaatt
    13801 ttttcaagaa atgaaagtta tgacaagaaa taaaattctg acatatgctt gctatggtga
    13861 tttgaatttt gttacaagcg gaacgttcaa aaaaattgat gagaccgata accctttggg
    13921 gacgattacc ttggcagaat ttaaaaataa tacgaatcgt tattgtgatt ttgaagtagc
    13981 tgaggaaatt ggagatgcaa ttgatcgtgc tttgtctgat caacaaaacg aaatcgaaca
    14041 atttaagctt gcgtacatgc ttgttactgg cacaacaatg agcaaaggca ctgctaaaga
    14101 aatgatgaat cagctaggga ttatcaactt aaaagcccct actgcaaaag ctgaatatgt
    14161 cacaaaaaat ttagcaaaag atttcaatga atatcatatg gatctattga aaaaacagtt
    14221 ttacaccatt tgtaaagcaa tcgattttaa cgatgaggtg tttaaatcaa acagttctgg
    14281 agaagctcgc aagtggcaaa tcattagcct agaagcgaaa acaaatacta aagagcaata
    14341 ttttagagaa ggattgaaag aatgcgcaga aacaattgct gcttttctta agtttcatga
    14401 caaagtagaa attgagcctg aaaaaattat ttttactttc tctagatctt tgccaactga
    14461 tttaagttat ttagctgaag ctttacctaa acttgcacca tacgtatcca aacgaactat
    14521 tcagagccaa attccatttg taacagatgt tgattatgaa aatgaaatga tggaattgga
    14581 aagtggaagt gcttatccag atagtgaata caattttggc ggaggtggca atagtgacga
    14641 tagaaacgaa gtattggacc aaacgtcgag aactggagga tcaagcaagg ctcaaacaag
    14701 aaaatcagac acttaaaaaa ttaactagtg tatttcctga agcactgaaa gagatacaag
    14761 caaaactatt atcacaagct gacttacaca atatcactta tccagaaatg atggagtttt
    14821 acagtacaag taatcagaaa aaatatcgtg aatatgtgga aaaaaattat aagtcattaa
    14881 aaatgtatga tgcaaaatac aaagagttta tcgatgaatt ttttccacca tttgactatg
    14941 caaaagtcaa tcgcttatta caaatacgat cagatgtatt taaaattctt gcagaatatg
    15001 cgatggatgc agatgtgaat caatattttt ctgatcgctt agaggaaatt cttcaaagaa
    15061 catattcttc taatgctaat gtttttgttc aacttttaaa cgttgatata ccaaattatt
    15121 taccagaaaa tgaaattaaa cattatttga actatccatg gtcaggaaag acattttcaa
    15181 gaagactttg gggaaatatt tcatcgcttg aacagaaact ctctaatgct attgtaaaaa
    15241 gtgttgctag tggagaaggc gttatacacg cattaaacac catgagatta gactcagaaa
    15301 tttgtgacat gtttaagtta gaagaatcaa agtataacaa agcgatagaa aatctcgttc
    15361 gaacggagta tgcaaaattt gcacaagatg gtattgaaaa atcatattta gaaacaggta
    15421 ttgaggaata caacgtattg actgcaaaag atgagagagt ttgccggatt tgtggaggaa
    15481 aggcaagtaa gaatccctat aaactgaaag atgctgtcat aggtgaaaat cgagcacctt
    15541 tccatagtcg ttgtagatgt acggatgttc ctaatttacc aaaattagga aaggatattg
    15601 atgaagaata tgaccgttta tttggcgatt tattagatga gtttgcacat gattcttttg
    15661 gaattaattt gaaacggagg aagtagaatg aaagattttt ttgaagcagt actaacaatt
    15721 aatgtaaatg ctgatattgc agaagcctac aaaacagcta ttgagtctga gaaccatcct
    15781 aatggcttga gagaccattg gaatggcaat tatgcctacg tggttattgg cgatcaaact
    15841 gttaattatc aagataatac tccagttgat aagaataccg ttaatttaac gattcaatta
    15901 ttatctcatt cattaccaaa tttaaaagaa acagttgatt ggtatgaaaa gatgggatgt
    15961 attgttgtta gaactgacta caaagaagga aagtctagta attaggcttt tttattttgt
    16021 ccgaaatgac gttaaactag cgcaatactg ggcttggttg aatggtgggg cgcaactatt
    16081 aaaactcaaa gcaatgcggg gcgtgaaaac gaatcgtggg gcgaaaggag aatgagcatg
    16141 aaacacaaat cattaatgcc aatgaatttg caatactttg ccgaaggtga cgatcaaaag
    16201 ttttcttttg atgacttcaa atcttttgtg gaatccaatg aagaagctca aaaatttatt
    16261 cagtcacaat ctcaaagtgt tgccgacaaa caattggaag cttggaaaca aaataactta
    16321 gataaaatta aacaggatac catcaaggaa tatgaggaat ctaagaaaaa caagtcacct
    16381 gagcaaattc aactggaaaa actacaagct gaatttgaag cagaaaaagc gttgcgtgtg
    16441 acaagtgata ataaagcatt tgttgcagaa caaattgctg gattagaact agatggagag
    16501 ttaaaagagt ctatttctca atttatgcta aataatcttg ttagttcgga tacagatttc
    16561 actaagaatg ctgttgaagg ttttacaagt gttttgaatg caattaatga gaaacatgca
    16621 gatgcattaa aagaactaca aatgaagtct gcatttggtg gaactcaaca atcgaataac
    16681 caggttcagc agaacaatga aacattaaca aatccagaag aacaattagg acaaattctt
    16741 caacaattta actaggagag tgaaaaatta tgaaaaaaac atctttaaat aatttagagt
    16801 atttggatat ttcaccagcg attaatgcta tgcaagtacc aaatacacct tttttaagct
    16861 atttacttgg tgctggaaaa acagagccag caaactcgac agaaattaaa tggcgtgaat
    16921 atgatatcaa caacgatgat tcttctgaaa aacttgaggg cggagaatat ccagatgctg
    16981 aatcaggtcg aacttggttt aacaactata ctgaaatttt tagaaaatca acctctgtat
    17041 ctggtacatt agatgctatt aatgtgaatg gagtcggaaa tgaattaact aatcaagtag
    17101 cactacgtgg tatggaaatg aaaattgatt tgaaccgaaa attgattact ggtgtaaaag
    17161 ctgatgaaaa tggttctaaa ggtcgtcgaa tgaatgggat tttgaacttg atcaattcag
    17221 caaataaggc agaaacagcc actgcgggtg cagtaacaag aaaagatatc gatactttat
    17281 ttaaattgat gtatgaaaaa ggttatatgg gagaaaaact atgcttgatt tctccagata
    17341 tgcaggagtt aatgactgat gagttagatg gaaaatcaac aaaaattgtt cagttcgggg
    17401 aaagagtaac ttttggattg caaattggaa atatcgtgtc taattacggt acaggtattg
    17461 ctctactaga accatcattg ccaaaaggaa caattgccgc aatcgatact aattatgtga
    17521 aactacgtcc attacgtgaa tggagagcag aagaacttgc aaaaacaacg gattctagac
    17581 gtatcggtct tgtaggtgaa tactctcttg aatacaatgc ttcaaactct ggggcaattt
    17641 taaatttaaa gtctgaataa aagggagtta gtactccctt ttttgatagg aggaattaca
    17701 gtggtaaaaa aagatgaaac taaaaaagat gaagtcgtga aatatagagt aggtaaaact
    17761 aaaaattttg ttgggtttgt tcatcctaaa actcgtagat ttatcacagc agattcaaat
    17821 aacgaattta tcatttctat agatgataaa gaagcaattg caattttgga agatgcaatt
    17881 gatgttaatg aaatttagga agtgatctga tggatgaatc gctaaaaacg gaaatcattg
    17941 agtctacaaa agaagatttt ccagatttga gtgaagaacg cataactaat ttattagaaa
    18001 taattttgct agaaattgaa tcatacaaca cttgtaaaaa tgatatttca tgggaaaagt
    18061 taaaaagcgt gattagtgaa gtgttgtata aaataataaa aaatgaatca gaaaaaacag
    18121 tatcttcagt tagacgtggc gatacaacga ttagttatgc ttcaacaaca aatgatgtta
    18181 gtgagttact tctaggctat ggtgatttga tacgaagagt tattggttgt ggaggattgg
    18241 agttttttta atgaatgaag cagatatttt agaaactact tatgaagata gttgtattat
    18301 tgaaagactg acggacattg aagattctaa tacaaatatt actattcaag attacaaaaa
    18361 agtatatgat aatcctattt cttgcgctct ttcacaaggt caaatcgatg gactagcagt
    18421 catagaagat ggagagatgg taaatgtttc aactgacaca tataaattat ttgttcatcc
    18481 taagattaaa ctaaaaaaag gagatcgaat aacaataact caaaaggcca gtggcttaat
    18541 tttttctcta tttgctacta agccttttta ctatcctagt cattgtgaag tgaatttgat
    18601 aggaagtgag aaaaatgggt aatctcaaat atgaatcaaa tgcagaaaag atcattgaga
    18661 attttaaaaa tatgactgta attgctcaaa aagaaggaat atccttcgtt aacgattcaa
    18721 tgaataaagt cgttagttta gctaaaccat taactcctgt aaaatcaggt aatttaaggc
    18781 gaggatatcg tgtagtaaaa gctagaaaac tatcaagcgg tcgaattgtt ggagaagtta
    18841 taaacaatga acattatttt aaatatgtag aagaaggtca taggactaaa aatggtggct
    18901 ttgtcaaagg gaaattcatg ttaactcgtg caacgaatct tgcaaatatg tcttatattc
    18961 ctcgaagatt taaacaaatg gcaataaaaa tcgttaagaa aggaaagtaa catgaaagat
    19021 gaaatcattg ctgcaatcag tagcaaatta aaagaaatct atcctgatgg gacaatatat
    19081 cttgattcag ttatgcagtc aactaaagat ttttatttcg ttttatcagt aatggaatct
    19141 ggaactgaaa atgtaggaat tgatattcaa aatgtttctt tcttaattga tattgcattg
    19201 attgataata accctaatag aaatttaatt aatgaattag tctcacgttg tgggactttt
    19261 tttaatacga ttacaatcga tgagcaaata ttatttccaa aagcctattt acctgatgaa
    19321 gcagatggtg ttcaacatat tcgttttaca ttagaatttc cacaatatat tgaatggagt
    19381 gaaagataaa tgggagaaaa aagaagtaaa accggaatta tttctgtcga aaaacctact
    19441 tggtttcctt tggaagatga gacagggaat tttccggtat atggtactgc gttgccaatg
    19501 ggaacagcag taagtatcaa accaacagct aattatgaaa caacacaaga ttatggggat
    19561 tcagtggttc aagatcaatt tacagctttt ggtggtgcag aagttgaact ggaagcaaat
    19621 ggatatactc ataaagtttt atctacaatt actggaggga aaattgttaa aggtggtgcc
    19681 ttgcgttctg gagaagatat tgcgcaagat ggtgcgtttg catatagacg taaaaaatcg
    19741 aatgggaaat accgttatac ggtcttttat aaaggacagt ttgctctaga ttcagatgaa
    19801 acatcaacga ttgaaggaag taaagtaagt tttactcatc caacttggaa gggttcattt
    19861 gtagatgtac caggacttgg atatatgtat tcagttgatg aagatgatga aggcgtggat
    19921 aaagcaatga tcggaaattg gtttacaaaa gtagcaattc caattgaaga agcagaactt
    19981 tcaggaggta cagaataatg tcaaaatatc aaacaacgat taaattaacg aaaaaagacg
    20041 aagaaggtaa atatgagcaa gtacaattta aatctgctga atttttacca ggaacagttg
    20101 tagaagatgc tgcaggagtt atggaagaaa tgcaaacagc gactgataag caatcagtta
    20161 aaaaagcttt aagtcgtgct tattcattta ttgcagatac tttatttgag ggacaattta
    20221 ctggtgaaga ttattgcaaa ggaattgatg ctcgtgagat tgcctcattg acaggaaaat
    20281 tattgaagtc tgttactgca ggttttgatg aaacttatac agagacgaaa aaaaagtaag
    20341 tgaggcgctc aagtcacctt catttaagta ttcgattact taccgagaat tagatataaa
    20401 gacgcaatta ctcgaagcag gttggacgtt accagaaatt gaacgtactg acttggatga
    20461 gttaatgcgt ctttttgctt ttagagatgc ggtaaaagag catgaagatg ttgagtacta
    20521 tgataatttc actcaatttt aggaggtgat actttgaata acgatgacct aattctgaag
    20581 atgatactag atgaatctgg ttttactgcc ggtatgaata atgctgtcaa aaagttgaac
    20641 tcttttgatg aaacaatcga aagaaaaagt agaaatagtg gcagttcatt aggtagtatt
    20701 tggaaaatat ttgctggaag ttttctagct agcggagtaa ctagaatcgt aggagctggc
    20761 tttgatctaa tcaagggttc cataagtgga gcggttgatc gagtagatac gatgaataac
    20821 gctctacgaa atttccaaaa tatgggattt agtaattcag aaattatgaa gaatatcgga
    20881 aagaatgggc ttttatctca aggtattcaa ggacttccta ctgctttgaa tgatgcgata
    20941 agtcatgttc aacttcttgc ttcttctaca ggtgatttaa ctcgttcaac tcaaatattt
    21001 aaagctttaa atgatggaat tcttggtttc ggtggttcaa ctgatcaagt taatgaagct
    21061 gttattcaat tatctcaaag tttctcaaac ggaaaagtag acgcacaaac ttggaactca
    21121 atgatcaacg ctcaacttgg acctactctt tctgctattg ctaaaaagat gggaattaca
    21181 atgggagatc tgaaagaagg tttatctcaa ggtaagattt ctgttgaaga attccaaaat
    21241 caattaatag aaatggatac caaaggtggc ggaggactta aatcattaag tcaaatcgct
    21301 aaagattcta ctaagggaat taaaacctct atacaaaatg caaaaacagc tgttacacgt
    21361 ggtgttggtg aggtaataga aggattaaat aaagcattag tagattcaga tttaggtgga
    21421 tttaagggaa ttattgataa agtgggcagt tcaatggaat cgttcttaaa agtaattgct
    21481 gcaaatattc ctacagcagt atcattttta agtaatttat ttaacgaaat tcaaaaattt
    21541 ggttctgctt tgaaattcat gatgcccttt cttgttcctg cagctaccgc ttttggagcg
    21601 ttcatgtttc aacttaaagg tataccagca ataataaaaa gcttcaataa ttttaagaat
    21661 gccataattg gcgttggaaa ttcattaaag attatggggg caatagctgc tgctaatcca
    21721 tttgttttaa ttattggtgc agtcgttggt gcaatagctg ttttcggtta ctttatggca
    21781 accaatgaag agcttagaaa caaggttata tctatttgga atgatgtgaa agattccgta
    21841 attggtgtat taaaaaatat aaaggactgg ggaattgata cttggaattc tgctaaagaa
    21901 atggcatcaa atgcagttga gggtgtcaaa gatgcttggt caggaacaaa ggaatggttt
    21961 tctaacacct ggaaaggtat taaggatggg gccacaggtt tatttgataa aacagtagag
    22021 acttcaagaa atgcagtcga tagtgtaaaa aatgcatggt ctaatatgaa gaaatggttt
    22081 tctgatactt ggcaaagtat aaaagattca gccagagaaa aatggaatgg aatcaaaggt
    22141 tctattatgt cagttgctgg tccattgatt acaggtataa agaatgcatt tttacatgtt
    22201 actttttatc tagagacctt atggaataat ttagttgaga ttggtaaaaa cgtctttgag
    22261 attttaaaaa atgttatact tgcgccggtt ttgtttatta cctccatgat ttctggtgga
    22321 tgggaagaaa caaaaaataa tatgattgct gtttggaata atattaaaga aagtgccata
    22381 aatatttggg aatctataaa gaatatattt gtaagttatt ttacaaatat ttattttgct
    22441 gcactcaata tttggacagg gttcaagctt acattgataa atatttggaa tgaagtggta
    22501 aatcaagcta
//
LOCUS       DARBFO010000037        22031 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000037 DARBFO010000000
VERSION     DARBFO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.038"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            367..555
                     /locus_tag="SCK51_001546"
     CDS             367..555
                     /locus_tag="SCK51_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252182.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="SCK51_001547"
     CDS             545..805
                     /locus_tag="SCK51_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252183.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="SCK51_001548"
     CDS             846..1073
                     /locus_tag="SCK51_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252184.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="SCK51_001549"
     CDS             1138..1710
                     /locus_tag="SCK51_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252185.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="SCK51_001550"
     CDS             1711..2022
                     /locus_tag="SCK51_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG1252186.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="SCK51_001551"
     CDS             2013..2273
                     /locus_tag="SCK51_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252187.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="SCK51_001552"
     CDS             2434..2793
                     /locus_tag="SCK51_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252188.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="SCK51_001553"
     CDS             2790..4433
                     /locus_tag="SCK51_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG1252189.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..5550
                     /locus_tag="SCK51_001554"
     CDS             4468..5550
                     /locus_tag="SCK51_001554"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252190.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="SCK51_001555"
     CDS             5594..6766
                     /locus_tag="SCK51_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG1252191.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="SCK51_001556"
     CDS             6753..7457
                     /locus_tag="SCK51_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEG1252192.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="SCK51_001557"
     CDS             7462..8622
                     /locus_tag="SCK51_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG1252193.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="SCK51_001558"
     CDS             8698..8976
                     /locus_tag="SCK51_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEG1252194.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="SCK51_001559"
     CDS             8957..9328
                     /locus_tag="SCK51_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEG1252195.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="SCK51_001560"
     CDS             9344..9748
                     /locus_tag="SCK51_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252196.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="SCK51_001561"
     CDS             9745..10155
                     /locus_tag="SCK51_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252197.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="SCK51_001562"
     CDS             10216..10803
                     /locus_tag="SCK51_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1252198.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="SCK51_001563"
     CDS             10876..11343
                     /locus_tag="SCK51_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HEG1252199.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="SCK51_001564"
     CDS             11389..11697
                     /locus_tag="SCK51_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252200.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="SCK51_001565"
     CDS             11892..18626
                     /locus_tag="SCK51_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEG1252201.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18630..19502
                     /locus_tag="SCK51_001566"
     CDS             18630..19502
                     /locus_tag="SCK51_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HEG1252202.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            19499..20548
                     /locus_tag="SCK51_001567"
     CDS             19499..20548
                     /locus_tag="SCK51_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252203.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="SCK51_001568"
     CDS             20552..21376
                     /locus_tag="SCK51_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252204.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..>22031
                     /locus_tag="SCK51_001569"
     CDS             21394..>22031
                     /locus_tag="SCK51_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEG1252205.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         7878 a         3297 c         4671 g         6185 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacgtataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg a
//
LOCUS       DARBFO010000038        22026 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000038 DARBFO010000000
VERSION     DARBFO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.039"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(61..1371)
                     /locus_tag="SCK51_001570"
     CDS             complement(61..1371)
                     /locus_tag="SCK51_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEG1252206.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="SCK51_001571"
     CDS             complement(1383..2801)
                     /locus_tag="SCK51_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1252207.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="SCK51_001572"
     CDS             complement(3041..4609)
                     /locus_tag="SCK51_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEG1252208.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="SCK51_001573"
     CDS             complement(4613..4894)
                     /locus_tag="SCK51_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEG1252209.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="SCK51_001574"
     CDS             complement(4891..5814)
                     /locus_tag="SCK51_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEG1252210.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="SCK51_001575"
     CDS             complement(5811..6689)
                     /locus_tag="SCK51_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEG1252211.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="SCK51_001576"
     CDS             complement(6676..8013)
                     /locus_tag="SCK51_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEG1252212.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="SCK51_001577"
     CDS             complement(8016..9119)
                     /locus_tag="SCK51_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG1252213.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="SCK51_001578"
     CDS             complement(9193..10701)
                     /locus_tag="SCK51_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1252214.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="SCK51_001579"
     CDS             complement(10667..11359)
                     /locus_tag="SCK51_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1252215.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="SCK51_001580"
     CDS             complement(11381..12355)
                     /locus_tag="SCK51_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1252216.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="SCK51_001581"
     CDS             12655..13527
                     /locus_tag="SCK51_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1252217.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="SCK51_001582"
     CDS             complement(14109..16307)
                     /locus_tag="SCK51_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1252218.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="SCK51_001583"
     CDS             16539..17531
                     /locus_tag="SCK51_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1252219.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="SCK51_001584"
     CDS             17591..19012
                     /locus_tag="SCK51_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEG1252220.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="SCK51_001585"
     CDS             19005..20504
                     /locus_tag="SCK51_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEG1252221.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="SCK51_001586"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="SCK51_001586"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEG1252222.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6669 a         4494 c         3823 g         7040 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaa
//
LOCUS       DARBFO010000039        21988 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000039 DARBFO010000000
VERSION     DARBFO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.040"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..395
                     /locus_tag="SCK51_001587"
     CDS             <1..395
                     /locus_tag="SCK51_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252223.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="SCK51_001588"
     CDS             398..1147
                     /locus_tag="SCK51_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1252224.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="SCK51_001589"
     CDS             1159..1428
                     /locus_tag="SCK51_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252225.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="SCK51_001590"
     CDS             1433..1597
                     /locus_tag="SCK51_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252226.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="SCK51_001591"
     CDS             1590..1892
                     /locus_tag="SCK51_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252227.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="SCK51_001592"
     CDS             1889..2050
                     /locus_tag="SCK51_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG1252228.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="SCK51_001593"
     CDS             2047..2352
                     /locus_tag="SCK51_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252229.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="SCK51_001594"
     CDS             2352..2708
                     /locus_tag="SCK51_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HEG1252230.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2695..2913
                     /locus_tag="SCK51_001595"
     CDS             2695..2913
                     /locus_tag="SCK51_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252231.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="SCK51_001596"
     CDS             2910..3329
                     /locus_tag="SCK51_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEG1252232.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="SCK51_001597"
     CDS             3326..3883
                     /locus_tag="SCK51_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEG1252233.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="SCK51_001598"
     CDS             3880..4176
                     /locus_tag="SCK51_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252234.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="SCK51_001599"
     CDS             4253..4666
                     /locus_tag="SCK51_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEG1252235.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="SCK51_001600"
     CDS             5012..5386
                     /locus_tag="SCK51_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252236.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="SCK51_001601"
     CDS             5423..5590
                     /locus_tag="SCK51_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252237.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="SCK51_001602"
     CDS             5616..5960
                     /locus_tag="SCK51_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG1252238.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="SCK51_001603"
     CDS             5965..6246
                     /locus_tag="SCK51_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252239.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="SCK51_001604"
     CDS             6349..6663
                     /locus_tag="SCK51_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEG1252240.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="SCK51_001605"
     CDS             6641..8335
                     /locus_tag="SCK51_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG1252241.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="SCK51_001606"
     CDS             8355..9533
                     /locus_tag="SCK51_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG1252242.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="SCK51_001607"
     CDS             9496..10182
                     /locus_tag="SCK51_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEG1252243.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="SCK51_001608"
     CDS             10182..11342
                     /locus_tag="SCK51_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG1252244.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="SCK51_001609"
     CDS             11352..12227
                     /locus_tag="SCK51_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252245.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="SCK51_001610"
     CDS             12224..12535
                     /locus_tag="SCK51_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252246.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="SCK51_001611"
     CDS             12525..12878
                     /locus_tag="SCK51_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEG1252247.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="SCK51_001612"
     CDS             12868..13269
                     /locus_tag="SCK51_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252248.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="SCK51_001613"
     CDS             13262..13666
                     /locus_tag="SCK51_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252249.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="SCK51_001614"
     CDS             13678..14286
                     /locus_tag="SCK51_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1252250.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="SCK51_001615"
     CDS             14306..14668
                     /locus_tag="SCK51_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252251.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="SCK51_001616"
     CDS             14671..14853
                     /locus_tag="SCK51_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252252.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="SCK51_001617"
     CDS             14870..18289
                     /locus_tag="SCK51_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEG1252253.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="SCK51_001618"
     CDS             18340..19077
                     /locus_tag="SCK51_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1252254.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="SCK51_001619"
     CDS             19087..21378
                     /locus_tag="SCK51_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1252255.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="SCK51_001620"
     CDS             21402..>21988
                     /locus_tag="SCK51_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252256.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3245 c         4705 g         6105 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DARBFO010000040        21911 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000040 DARBFO010000000
VERSION     DARBFO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.041"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="SCK51_001621"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="SCK51_001621"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEG1252257.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="SCK51_001622"
     CDS             complement(1172..1867)
                     /locus_tag="SCK51_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEG1252258.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="SCK51_001623"
     CDS             complement(1880..2596)
                     /locus_tag="SCK51_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEG1252259.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="SCK51_001624"
     CDS             2926..3534
                     /locus_tag="SCK51_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEG1252260.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="SCK51_001625"
     CDS             3859..4182
                     /locus_tag="SCK51_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEG1252261.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="SCK51_001626"
     CDS             4364..5734
                     /locus_tag="SCK51_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEG1252262.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="SCK51_001627"
     CDS             complement(5864..7165)
                     /locus_tag="SCK51_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEG1252263.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="SCK51_001628"
     CDS             complement(7519..9477)
                     /locus_tag="SCK51_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEG1252264.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="SCK51_001629"
     CDS             complement(9654..9920)
                     /locus_tag="SCK51_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1252265.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="SCK51_001630"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="SCK51_001630"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEG1252266.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="SCK51_001631"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="SCK51_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEG1252267.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="SCK51_001632"
     CDS             13264..13827
                     /locus_tag="SCK51_001632"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEG1252268.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="SCK51_001633"
     CDS             complement(14080..14601)
                     /locus_tag="SCK51_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEG1252269.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="SCK51_001634"
     CDS             complement(14604..15446)
                     /locus_tag="SCK51_001634"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEG1252270.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="SCK51_001635"
     CDS             complement(15439..16164)
                     /locus_tag="SCK51_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEG1252271.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="SCK51_001636"
     CDS             16340..16636
                     /locus_tag="SCK51_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252272.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="SCK51_001637"
     CDS             17204..18796
                     /locus_tag="SCK51_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEG1252273.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="SCK51_001638"
     CDS             18809..19549
                     /locus_tag="SCK51_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1252274.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="SCK51_001639"
     CDS             19717..21066
                     /locus_tag="SCK51_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1252275.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="SCK51_001640"
     CDS             complement(21194..21784)
                     /locus_tag="SCK51_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEG1252276.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6294 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa a
//
LOCUS       DARBFO010000041        21785 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000041 DARBFO010000000
VERSION     DARBFO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.042"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(109..423)
                     /locus_tag="SCK51_001641"
     CDS             complement(109..423)
                     /locus_tag="SCK51_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252277.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            709..948
                     /locus_tag="SCK51_001642"
     CDS             709..948
                     /locus_tag="SCK51_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252278.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1310..1570)
                     /locus_tag="SCK51_001643"
     CDS             complement(1310..1570)
                     /locus_tag="SCK51_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252279.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1755..2252)
                     /locus_tag="SCK51_001644"
     CDS             complement(1755..2252)
                     /locus_tag="SCK51_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEG1252280.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(2382..3092)
                     /gene="budA"
                     /locus_tag="SCK51_001645"
     CDS             complement(2382..3092)
                     /gene="budA"
                     /locus_tag="SCK51_001645"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEG1252281.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(3105..4769)
                     /gene="alsS"
                     /locus_tag="SCK51_001646"
     CDS             complement(3105..4769)
                     /gene="alsS"
                     /locus_tag="SCK51_001646"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEG1252282.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4977..5639)
                     /locus_tag="SCK51_001647"
     CDS             complement(4977..5639)
                     /locus_tag="SCK51_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1252283.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5649..6464)
                     /locus_tag="SCK51_001648"
     CDS             complement(5649..6464)
                     /locus_tag="SCK51_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1252284.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6725..7168)
                     /locus_tag="SCK51_001649"
     CDS             complement(6725..7168)
                     /locus_tag="SCK51_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEG1252285.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(7199..7312)
                     /locus_tag="SCK51_001650"
     CDS             complement(7199..7312)
                     /locus_tag="SCK51_001650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1252286.1"
                     /translation="MEADSSNTLALLLEKLIRDYPSVQFHIHSGTSDDITE"
     gene            complement(7302..7640)
                     /locus_tag="SCK51_001651"
                     /pseudo
     CDS             complement(7302..7640)
                     /locus_tag="SCK51_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(7628..8005)
                     /locus_tag="SCK51_001652"
     CDS             complement(7628..8005)
                     /locus_tag="SCK51_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1252287.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            8229..9422
                     /locus_tag="SCK51_001653"
     CDS             8229..9422
                     /locus_tag="SCK51_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1252288.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9588..10010)
                     /locus_tag="SCK51_001654"
     CDS             complement(9588..10010)
                     /locus_tag="SCK51_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1252289.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            10593..11087
                     /locus_tag="SCK51_001655"
     CDS             10593..11087
                     /locus_tag="SCK51_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252290.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(11249..12421)
                     /locus_tag="SCK51_001656"
     CDS             complement(11249..12421)
                     /locus_tag="SCK51_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1252291.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12627..13442)
                     /locus_tag="SCK51_001657"
     CDS             complement(12627..13442)
                     /locus_tag="SCK51_001657"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1252292.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(13592..14038)
                     /locus_tag="SCK51_001658"
     CDS             complement(13592..14038)
                     /locus_tag="SCK51_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEG1252293.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(14035..15039)
                     /locus_tag="SCK51_001659"
     CDS             complement(14035..15039)
                     /locus_tag="SCK51_001659"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEG1252294.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(15079..15408)
                     /locus_tag="SCK51_001660"
     CDS             complement(15079..15408)
                     /locus_tag="SCK51_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEG1252295.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(15534..17861)
                     /locus_tag="SCK51_001661"
     CDS             complement(15534..17861)
                     /locus_tag="SCK51_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1252296.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            18503..18616
                     /locus_tag="SCK51_001662"
     CDS             18503..18616
                     /locus_tag="SCK51_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX protease"
                     /protein_id="HEG1252297.1"
                     /translation="MYAPGTILLTIAYLTANRSLAFIIAIHILNNVLGFVL"
     gene            complement(18704..18997)
                     /locus_tag="SCK51_001663"
     CDS             complement(18704..18997)
                     /locus_tag="SCK51_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEG1252298.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(19400..20578)
                     /locus_tag="SCK51_001664"
     CDS             complement(19400..20578)
                     /locus_tag="SCK51_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1252299.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(20682..20996)
                     /locus_tag="SCK51_001665"
     CDS             complement(20682..20996)
                     /locus_tag="SCK51_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1252300.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            21103..21321
                     /locus_tag="SCK51_001666"
     CDS             21103..21321
                     /locus_tag="SCK51_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252301.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            complement(21316..21711)
                     /locus_tag="SCK51_001667"
                     /pseudo
     CDS             complement(21316..21711)
                     /locus_tag="SCK51_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         6452 a         4265 c         3673 g         7395 t
ORIGIN      
        1 aataattgtt cactcaaaag tttttctaga acaattaaaa cagcaggctt cacattcgct
       61 atacatttgt aagaagtaat aattcctgta ctaaaaggca taattgcttt aattattttt
      121 tgacttctct tcaaacacaa gaagtagttg tgtaataatt gcagaaataa ggattccaat
      181 aatagatata aaatttgaca tggaaacgtc tataacatta aaagaataaa gtgttgaaga
      241 aagtaaactg attgaaataa cccctccgta aaaaatagtc tgtgaatata cccacttttt
      301 ttcagaagac gtacttcgtt tggttctaaa acctatccct gcatttctac tcttatcaaa
      361 aaaccaaata tttagaagta ataatattgc tattattaag ataaaattac tgtaaaaatc
      421 caaaagctat cctcctagca actatttaaa tacccaaaaa aattacctct agataatatc
      481 atacctacgt tttcacttat aaattttttc ataaaaaaag cgtttagagg cataatcgtt
      541 aatacagatg gtaaccaatg tatctggttt gaactgggat atcaccctct ccatatatgt
      601 attactaaga ctaatagata ctataaaaga cttgatagtt ttgcattgat tttgcctaat
      661 actgagcata ggtgtatgct aaacgatact ataaggagtg tgtacataat gaaaagaatt
      721 ctgtttatca gtttatcaaa tttattgtta cttactgctt gtgggacttc tgcatcaaag
      781 aacatacctt cagatacgca aatttcgaat agtatcgatt caacaatttc tacaaatgag
      841 aaaaattctc cggtagaaac ttccgatata aaagtttcta cctctgcaaa aaacagggta
      901 aagaaactgt cagcacgtct cctttatttc aaacaaactc tgaattagct tcttcatctt
      961 cttcagcaaa taatgatgat agtttcgcgt ttactcctaa ctaaatgcaa cctttacaaa
     1021 tgctttacga taacaagcct gagctaaatg atcctgatat cagttttgca ttttttgaaa
     1081 tgattggatc ggatgatctt tttaaaacta ctagtttgtc tatccgaaaa taggacagtt
     1141 ctggcagtat aggtttttat cgagtttctc caccaggcct cgttacagag acagattctc
     1201 taggcgaacc ttttgaataa atcaaaaatc atgaacaatc actgccaact aaataaagtg
     1261 ccttcaaaag cttggtcttc tcgcttttga aggcacttta tttaaatctt tatgaaacta
     1321 aagtatgttc atatttgttg ctatagttat atagatattc tgttgtttcc aactcataaa
     1381 cgtgagtata ttttgccact acttttccgc atttttctaa tatttcattg cgatcgacgt
     1441 catgatattc gtctacgcag atcacaacac aattttctaa tttactgatt acttctccaa
     1501 ccactttttg tttcaagcca ataggttggc aaatatattt ttttcctaaa atgatagttt
     1561 cgttattcat atttcatcca tccttaatgt tttttcatta ttcaaaatga taataccatt
     1621 aaaatatgat tgtgaaaatc aataacactt gtgattttat aaaaaaataa aaaaaccgaa
     1681 aaacagaaat gaccactgtc tttccggctc tttttattct tgatttctct tcttttttgt
     1741 ttcaatagac gattttagtt attaataaat gaaaaagcat ttatattaaa atttgctaat
     1801 aaaagatcta catatttttc tagaaatgct tgatcgattt catcataccc tttcgttact
     1861 ccgcaatcga tatctaaaac accgattaaa ttaccttctt ttaccattgg cactacgatt
     1921 tctgattgtg cggctgaatc gcaggaaatg taatttgcat gggttttgac atcatcaacg
     1981 atcaatgttt cttgtttttc agctgattct ccacaaactc cttttcccat cttgatacgt
     2041 gtgcaagaaa ctcgcccttg gaacggtcct aaaatcaatt cattaccatc gaataaataa
     2101 tatcctgcaa agacagtctc aggcaaagtc tcattcaaca atgcagatga atttgctaaa
     2161 tttgctaacc agttatcttc tgcttctaat aatccttgtt gctgtaataa taaaatttcg
     2221 tatgcatctt ttttctgttt ttctgtccac atgataaaaa ccgctccttt ttgatctatg
     2281 gagaagatta tagctcagtt tttttgtaaa cgctaggttt cctcctcaaa aaaacgagat
     2341 acgtcaggtt aatcaccttg aacgcatctc gctgtctttt tctattctgc ttgttggatt
     2401 tctgctgcta aatcagcata atcaatcgtt tcgtgaacaa aagtcggatc atcaactgga
     2461 aaatgctgta caagggacga gatgcggcta atctcaactt ttccttcagc cacttcaaaa
     2521 tccattacat gtccgccaaa tgtatgtgta tcatcgataa aatgcaggtg aaatcccgct
     2581 gcagcaactc cttggaataa ttcaggcgta aagaacccga ctaatgttcc ttggatattg
     2641 ctttgttgga attctggttg agactctgaa atttttgcta gtcttctgta aggtttttct
     2701 tgcttcggca tgatacggat gtgcatattt ttgaataatc cagttatctt cactgccaaa
     2761 aataggtttt ccccggcttc tcttactaat atgtcttttt tcaattgttc tccatctgca
     2821 gtttttgaga cagaaaaact agtatctggc gtaaaatctg taattgctgc atagggggta
     2881 agttcacttc catctaattg ggcaactgag ccatctgatc ttccttgata agctacgcca
     2941 tctaaaaaga tgacttcacc atcaagccca tgcaatgttc ccaatcccag agttcctttt
     3001 tctaatatat gagcaatcgt ttctgttcca tccatcaagc cggccatcaa agctcctaat
     3061 gtcccgtgct ggtataagat tttttcagtc atgtcgacac ttccttaata gaattgatct
     3121 ggtaagattg tttctcccaa tttctcatta tcacggtaat caatcggaat atcaataatt
     3181 acgggtcctt ctgtcgcaaa tccttcttct aaagcttttt ctaattcctc tacacttgta
     3241 gctcggattc ctttagcgcc aaaagcttct gcatatttca caaaatcaac tggaccaaag
     3301 tctacacctg acgcccgctg atatttcatt ttttcttgga actctaccat attgtagtgt
     3361 ccatcattcc aaatcaaatg aacgatattc aattttttac gtaccgccgt ttctaaatcc
     3421 tgggcagaga ataaaaatcc cccatcgccg gaaactgata cgatttgtgt atttggacgc
     3481 accaatgcag cagaaatcgc ccatggcaat gcaacaccaa gcgtttgcat accattactg
     3541 aataacaaat gacgaggttc ataacttcta aagtggcgtg ccatccaaat ataatgacta
     3601 cctacatcta cggtaacagt catatcatct gttacttttg actgaagcgt gttaataact
     3661 tctaaaggat gcaagatacc tgcttctcct tttgattgaa caatgtcccg ctccgttaat
     3721 tttgcttgaa gagatgctaa atattctttt gcatcttctg acacttgttg aggctctaat
     3781 gaacctgtca ataaatcaag agtggctgag atatcaccga tcaattctct ttcaggctgc
     3841 ataaaaggat cgatttcagc tggcgcttca tcgatcacaa tgatccgcgc atctttttcc
     3901 gcattccagt ttcttgcttc atattcgatt ggatcatagc caattgcaat aactagatca
     3961 cttcgtttca aaagcatatc tcctggttgg tttctaaata aacccacacg accaaagaaa
     4021 tgcgcttcta attcacgtga gatcactcca gctgcttgga atgtctctac aacaggcaat
     4081 gctgtctttt caactaattg cctgatggct aacgtttctt tttcacttga tcctcgcata
     4141 cctactaata gtaccggtag ttttgcttct ctaatgcgtt cagctaggta acggatatca
     4201 tctgcagaag cagaacctaa tttaggatcg ctcaatggct tgatgacatt accttggaca
     4261 ggtgcatcca ctacatcttg cgggatactg ataaagctcg cgcctttttt tgaactctta
     4321 gccatacgat aagcattggc aatcacttct gaaattgttt ctggatcttg gatttctgca
     4381 ctgtattttg tgattggctg gaacagtgct gcattatcca tgctttgatg ggtcaacttc
     4441 aatagatcgc ttcgttttac ttgtccggca attgctaaaa ctgggtcacc ttctgctgtc
     4501 gctgttacca aacctgtagc taagttacta gcaccaggac cacttgttgc gataactact
     4561 cctggctctc ctgtaatacg tccgattgct tgtgccataa atgcagcatt ttgttcgtga
     4621 cgagtcacga ttaattcagg accttgatcc tctaattcat taaatacacc atcgattttt
     4681 gcacctggta taccgaatac ataatcaaca tgatgattaa tcaaactttc tactacagat
     4741 ttcgaacctt gtttcatttt ttcagccatt gaacatcgtc ctttttatgc tttattttta
     4801 attattagtt agaaataatc acaaataagt gatacagttt ctaaacctgt ttcatacaaa
     4861 agtatagcac gttttatttc atttgtttag ccatatgttt caaaaaatga aaaagaatga
     4921 cagtttttac ataaaaaaaa ctgtcattct taattgaaag tttcaattta taaagtctac
     4981 agatccatag ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa
     5041 ggacctagta gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat
     5101 agtaaaaaat ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt
     5161 agaacttgat ttggtttcga cgctggattt ctcacctgaa aataccagca gttggtgcta
     5221 aaggaaactg ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa
     5281 aaacgtataa aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc
     5341 caataaaaaa atccctttag ccaaaattgg cgaaacatct ttatgaacaa acgtactggt
     5401 gaaaaactat tttcttttgc taattcttct gtcataccta tcatcacgat gcaacctgga
     5461 ccaataaaaa gtaaagaaaa aataaaggaa aagacattcc ttccatctat cgacaaaagg
     5521 aacgcagctg ctatatacaa gatattgaca caccaaaaac aaaggctact tgccaatgat
     5581 tgatacaacc aataaaagaa ttccatataa acatttttgt caaaacgctg ctctttcata
     5641 atcacctcct attccttcat tcctgatgaa gcgatccctt ctacaaaata tttttgcatc
     5701 gctaaaaata taaaagcaac aggaagaaca gaaacaactg aggcagccat gattaatgcg
     5761 tactctgcat caaatagccc aataaacatt ttcaacccta actggattgt tttattttcc
     5821 gtagaagaca aataaatgaa tggccccata taatcgttcc atgtattaac gaatgtgatg
     5881 atcgtcaaac tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg atagattccc
     5941 cactctgata agccatcgat ccgcgcactc tcacataaag aatctgggat actactgtaa
     6001 tattgcttca acaagaacac accaaaagca ttgaacgctt gtaatagcac caacgaccac
     6061 aacgtatctg ttaaacccac ttttctcatc ataatgaact gcgggatcat ataagactgc
     6121 caagggacag caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc tttaaaattt
     6181 agtttagaaa acccgtaagc agcaaaacta gaagtaaaca actgaatcaa cgtgatcaca
     6241 acgcttaaaa aagctgtatt cttaaaaaat gtcactaaag gaatacgaga ccaaataacc
     6301 agataattct ctaaatgcca agttttaggg atccattgaa tcggaatcgt gaacacttca
     6361 ttatttgctt tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt caataaagct
     6421 aatacaatca gcagaatcat acccaaccat tgaataaagg gcatgttttt tgaacgaatt
     6481 tttgcttttt ttctttcatt tcgaacacct ccaaagttat aatcttcatt tctttgtaat
     6541 gattacaatc cagaacaacg gttattatag ctgattcatt tccgttcttc ttatttttgt
     6601 aagcgcttat ttaacaagtt atgtaaatct ctttattttt ttgatttaaa aatgaaaaaa
     6661 tacaaaagca agactgtcat acgtctcgct tttgtattga aatttcttcg attagagata
     6721 cttattactg ttcaaaagct tctttgaaac ggtgaatcaa ttcattgact acaggtgata
     6781 aaatccgatt ctttttccag actagcatgc agttcgtttc aatcattggg tcaagcggaa
     6841 cgaatgtcac tcgctttgaa tgatgactta cagcaccttc tatgactaaa gcagcaccta
     6901 cctgattctc aattagcgca aatacattga aaatcaaatt gtacgttcct gtgatcagca
     6961 actgctcctt ttttatgcct aaccagtcag caaacattcg ctgtatttct ggacgctttg
     7021 agatcaaaag aggtacattc gcaaagtctt cttttgttat cacgtctttt tttgctaaaa
     7081 cagaattttc taaaatgagc aatccccatt tttcctttct tggtaacaaa atcgtttcgt
     7141 atttctctgt atctactgga tctagcaaaa tcgctaaatc taacaggctt ttttctagtc
     7201 actccgtgat atcatcactt gttccgctgt ggatatgaaa ttgaacgctg ggataatcac
     7261 ggatcaactt ttctagtaat agtgccaatg tattcgaact atcagcttcc acacagccga
     7321 tcgtaaatcg gccgctgaat aattgttttt ttctgttctc gaattcttga gtggtttgat
     7381 tcgttaaatc taaaatttct tctgctcggc ttttttagaa aataacctgc ttctgtcaag
     7441 atcattttgc ggttttcgcg tacaaacagt tctgttccta gttactcttc taactccttc
     7501 agttgtcggc ttaaggttgg ttgtgtaaga tgtagaactt ctgcggcttg agagattgtt
     7561 ccttcttctg caattgtcca aaaatatttt agtaagcgta actccatagc gatgctcctt
     7621 gattttttta cttgatacat tccattttat ttctagataa cacacgatca tatttcttga
     7681 tttttccttg taaacgttcc aaacattctt gcatttcagc aatttttttc gtcaaaagtt
     7741 cactttcttc aatcaaaagc ttacgccgct caaatacagt tgcttctcct tcgctagata
     7801 ggcgaatata ttcaatcaaa gactcgatcg ataatccggc atttcgcata cagatcgtaa
     7861 agtcgaccca ttttaggtct tcttctgtat aatcacggat tccgcctttt gtccgattaa
     7921 ctggtggaat caacccgatt cgttcataat agcgcaatgt atcggccgaa atgccctttt
     7981 cttcactgac ttgtttgata ttcatctgat cactctttct cttaatttct tgccacttta
     8041 cttatgtatt ttatttattc tacaacttta agtgtactcc aagtcaatac tcatttctat
     8101 taaaactaag ctggattatc tatatcggtt gtttgtgtat tatttcaaat aaaaaagtct
     8161 tgttttttta ttcttttttg gatatacttt taacaattta gggaaatttt aggaaaaggg
     8221 gtataaaaat gacaacgtat cagcgaaagg tattagtatc gacatcggcg ggaattgctt
     8281 tagagaatat ggatattatg tttttagctt tttcattatc ttcaatgatt acttcttttc
     8341 atattagtgg tacgcaagcg ggtttcattt caacgatcac taatttgggg atgctcattg
     8401 gaggtatttt ttttggcatc atagctgata aatatggacg tgtgaaagta ttttctcaaa
     8461 cggtccttct gttttcgatt gcttctttat tgatgtattt tgcttcaaac atctacttgg
     8521 tttatctctt tcgctttatc gcaggtgtag gagcgggagg agaatatggg gcgtgtatgt
     8581 ccttgatttc cgaaagtttt tcaaaaaaac aaattggacg agcttcttcg gttgctggta
     8641 ttggtgcgca ggtcggtgcg gctttggctg cgattttagc tgctgtgatc attccaacgt
     8701 ttggttggaa gatgttgtat gtcgtaggtg tactgccggt gttgatggtc ttgttcattc
     8761 gtcgcggctt gactgaacca aaggaattca aatctgtaca atctgccgaa aagaaaacca
     8821 aacttagcag actgtttgaa tcaaaacatc ttgcttggca aacatgtggc cttagtatca
     8881 tggtaactgt ccaaattgcc ggctattttg ggttaatgaa ttggctacct tcaattatgc
     8941 aaaatcgatt agggctttcg gtttctgggt cttcattgtg gatggtcagt actattttag
     9001 gcatgtcttt ggggatgatg acttttggca taatcatgga taaattgggg cctaaactag
     9061 ccttttcttt gtttttaatt tgttcatcct tatctgtcta tctacttgtg ttagcccata
     9121 gccagtggag tttggtagca gccgctgtga tagtcggcta ctttatcaat ggcatgtatg
     9181 gtggatatgg cgcagtgatt agtagtttgt atccaacaga aatccgcgcg acggcgaata
     9241 atttcgttat gaaccttgga cgagctgttg gtggtttttc ttctatcgtc atcggtttct
     9301 tgatggatca ttatagttta actgccgtaa tcttgttttt gagcggtatt tacctagtca
     9361 gtctgctggt cttgacaact cttactggtg ttacagcatt aagggaaagc ttgcagcatt
     9421 gatagttgag tctgtaaatg acaagaaaga ggtgtggaaa aagcttgcat ctgcggtaat
     9481 aagaccagcc aaatcaaaaa catgagacac tgtttccgtt tggctggtcg tattacttga
     9541 tgcagggcag ctttttcaac tgctaatttc cttctatctg gcggcgacta ttctttgacg
     9601 tccgtcattt caaagtactt tactaataac ttagcataac gaaaaactgt ggatacactc
     9661 gtatattgag cgtctgcata atcttccaaa tttttctcag gatgttgata catgaattca
     9721 atcaaatgat aattatatga ttttctgtag aaatgaagat aaataatgta gattgggaag
     9781 ttctcctgct tttccataga aaagcgccga ttattctcat tcttcaaaat gaccttatca
     9841 ggatattgtt ggaacaattc ttctaaatct gttagataat tcaagataga cttacttgat
     9901 accttcaatt cgttttgtag ttcttctatt gaacaccaac gttctgtttc aagcagtaaa
     9961 tgaatcattt taattttctt ttgagaaact tttgttaaaa aagtctgcat attttctcct
    10021 cctgtatcta ctttactata ctacatacaa gaaaaaaacg gatacaaata tcatattgtc
    10081 acaccgtcaa tagtgcataa acctgttatt atttctcttt ttttcctaag tattctattg
    10141 atatgaatca tttaatcgtt tattatataa atgtcaataa tctaataata aaataacgta
    10201 catattttat tattgcatac ttaataacag aactatagca aaaaatcccg tgtatcaaca
    10261 cgggattaga ttaagtttcc tcaatcacta ttcataacac aattattata acaagtaagt
    10321 aaacaattga aaaggacatt tattgaaaac ggttgttttt tacttaagga ggaaatttag
    10381 tgaaaaaatt agttgcgttg tttttggtcg caggaagttt gttgagtggg tgtggtgcta
    10441 ctacgtctac tactcaaagc acaagttcag ctcaggcttc cagcagcagt accacgatct
    10501 ccgaaagtac ggcagattca tcgaaagaac aaattgaaaa gaaaaaagaa gaagagaaac
    10561 aaaaagcgga aaaagctgca ctagaaaaaa aagtgacaga agcggaatct gctttgtcac
    10621 aagctgaagc aaatcctaca aaagaaaact atgcaactgc ttcagcagct atccaagcgc
    10681 tacctgaaaa caaacaagag ttgacaagcc gtcttgctgc aattgattcc acaatcaaag
    10741 caaaagaagc agaagaagaa cggaaaaagc aagaagaaat tgcacgacaa caagcagaac
    10801 aagcgcaaca agaagcaatc gcacagcaac aagcagaaca ggcgaggcaa gaagcaatcg
    10861 cacagcaaca agcagaacag gcgcgacagc aagcagcagc tgaacaggct tctcagcagg
    10921 cacaagccgc agctcctgag caaaataatg aacaaactgt ctatgtcaca ccgaccggtt
    10981 caaaatacca tacacataaa tgtggaaatg gtacgtatac ccctgccact ttaagcgaag
    11041 ctcaagctcg tggtttaacc ccttgctcca aatgttatgg aaactaattc aaaagttata
    11101 ctataataat aaaattcttc tgaaacataa aaaaatagaa gctggcgttc cagatagtaa
    11161 accaaaaata gcagtttaca cgtccatttc gtcagcttct ctcttttttc atttagtctg
    11221 ttccctctgc ttttttggac atcttcattc attctttctc aatcaaatag tacacctctt
    11281 tttcttttcg cagtttatat cttccttttt tcaatttcat tgaaaactgt ttcgaatgtt
    11341 tatgttttac atggatttta agcaggagct cctcatgttg caagcgatag ttcctgcctt
    11401 ttaaatcaga aaatctcact agtccgtatt catcagaagc tttcacaaat ggaacttttt
    11461 ctctagtaat atagtgtttg ccgttgagcg tttttacaga aaagaccatt ccttcgattg
    11521 gtcgtccgtt tgtgttcttt acataaaaag ataaaggata gtttcgctta gcgatggcca
    11581 aagaatctaa ataagcgatg agtagccaaa ctaagccgat tgatccaagt agtataccga
    11641 taaaccaaat aaaaaatttc cattttttcc attgatcaat tccttggatc gctttttttg
    11701 cctcttttgc ttgataagga atacgatgcc ccgtcacaag cagtcgatgt gtattgacca
    11761 tataaggagt gcaggtaagc aaagtcacga gatctctgcc tttttggata cccaaagaat
    11821 ctacttcgtc aggtaagacc acttggattt tttctacttg atacgccaat gtctttccat
    11881 tgatctggat gaaaaactgg tctccttttt tcaacttagg caaatctgta aataattttg
    11941 cttctgggag tccacgatgc ccagaaagaa cggcatgggt gtttttcccg ccagtcggat
    12001 aagaacttcc ttccaataat gacgttcctt tttgcaaaaa tatttccgtt gtctgatcga
    12061 atataggtaa gctgacattg atttttggga tttcgatgac tccaagcata tgttccataa
    12121 aaaaggcatt actaggtaaa tcttttaatg cttttgcatc aactgcttgg ttgaaactgc
    12181 ctatccctgg tgatacgttt tgctctgcca actgttggtt tttctttgtc attttttcct
    12241 gctgtttttt tatttcctct gaattcttat tcgatgcttg cttttgataa tggatgatca
    12301 tttgctgggc aagaaaatca ttcagtgaat cttctacaaa aggataagaa aagatcccga
    12361 tcccaacgac taaaatgatt gccatgaaga tgtccagtat tcttattctg cttttattca
    12421 tcatgtccac ctattttcta atatgtatat gaagaggttg tgaaatcagc gccttgacct
    12481 gagtattagc gaagcaaacg gcaaaatagt tcctcctatt ttgccgtttg ttgagcgaat
    12541 accaagagac cgaccacttg tcacaacctt tctccccatt tgaatcacta tcgtttattt
    12601 atcgtatgat ttttgtttcc aatagtttaa gcttctgttt cttgatggcg ttttttgaaa
    12661 tagatcactg ctcctccaac taaagcagta cctaccaaga tgaatacgat gatccccata
    12721 ccaccagttg aaggcaagat tcctttaggt tggttatcga tcgttgtttc atcagaagtt
    12781 gttggatcat catctactgt aaattcagtg tacgcagctt cattcttagg taatgcgtag
    12841 ccttctggtg cttttgtttc ttgaaggcag tatgcccctt gtttcagccc ttcgattgaa
    12901 aatttccctt ctgaatcaga aatcaatttc acttcttccc agtcagaacc tgtgacttca
    12961 tctgaccaag catatgggta ttctcctgag gcaaggtttg ctattttttt cattaattta
    13021 acagttggat cttttgctct tagaatagaa aattctgcat cggctaattt ttcttgcgtg
    13081 ttcacatctt gtttgatgaa gtggaaatca ccgtagtttt ggacattttc tacagtaatc
    13141 gtgttatccg tagttgtatt ttcccgattg actacaccgt ttggattccc aagattggct
    13201 tcatctgcgc catggatgat accggttgtt tgttgcaatt ttccgttttt tactataaaa
    13261 gtagtttctt tttgtggatt ggttaattga taaccatctg gtgcttttgt ttcaactaaa
    13321 gtataattgc cgtctattaa tccattgaca ctgatggatc cttcagtatc agttgtgaat
    13381 gtcaacgctg actcagcagg atcatagaga gcgacaccag tgttttcatc aaaaccggtc
    13441 acgaattgat tctctgaatt tttgatctgg aattgcgccc ctgctaactt atctacccct
    13501 gaaaagttcc cagctttctg gattttgata ttgctttctt tgatgacatt tttagcatat
    13561 aggtgaatcg tgtctaaagg tttgtcaggg tctagggtaa gcttaacacg atatttggcg
    13621 acgtttcgac accgggcttt ggtgtttcta caaagacgta tacagcatct ttcccatcag
    13681 atttacctgg aagagtcagg ttagctaatc cttcaccaga agtcgttaca cttgcgaagg
    13741 gtgtagcgtc tgctggaact gctgtgtctt tacttggata gtacgtagca gtgatgtcat
    13801 atacgttgaa ttcaacgccg ttcaacggtt cgctattcgg aaatgtcaat tccgttccgt
    13861 tgtttatttg attttctggt gctgaagtga atttcaattt atgcaaatta accgtaacat
    13921 tttctggtgt aggtgatacg gctgcagaaa cctctgcagt tcctaggccg agaacgaaag
    13981 gaagcagcat gatagcagtt cctaaaaacg tccatatttt tgttttgatg tttttcattt
    14041 tatttccctc ctattgtttc ctttttgttg ttttttataa aaccaagctg tccctgttga
    14101 aaggactagc aataacccta aaattgagaa acctccaagc atctcttcgc ttgtttttgg
    14161 aaagcttcct ccattttttt catttgatgg accatttgtc tgcatctgaa ctggcgtttt
    14221 tccattattc gtcggatttg cttttggttc gttgatgatt tccaatggta taggatgaag
    14281 cggatcataa ctgccttttt caacttcaaa gagaatggtt gattgactca atatatatcc
    14341 tttaggcggt ttaatctcca ctagttcgta acttccaaaa gacaatccat ggacagaaaa
    14401 tgttccatct ttactggaag taaaaatcgt tgctcttgaa tcatctttgg ctacccaatg
    14461 atttatcccg ttttcttgcg ctaagtattg gtcatttttt ttgactagaa attgagcatc
    14521 tgctagtttt tgtttcgttt gccgatccac tttgacaaat tgtttgcctc ctgtttggat
    14581 ggcaagatgt gtttctactg tttgattgtc agtcgtcaaa cgtacatcgt tatcaaatgt
    14641 cgttttttca ctgttttctt tcaatgtcat ttggtaacgg atcgttaatt tttttccagc
    14701 aaacgaacta agatcttttc cagaatctga taagacaaaa ccatgtttta ttttttgtgt
    14761 gtgaaatgta tgaatcttgt ctccaccaat caaaaaatcg atcgtttctg gttctagcca
    14821 taactgagga tcagctgtat ctttgatcgt ataggttcct atctcgttga tgttctcagg
    14881 aattgttacg gtgatgagat aagggacttt atccccatct gcaaaatctg tctgcttatt
    14941 ctctactgtt tttgttaatg tgagtgcagc aggtattttt tgttcgctct ttggatacaa
    15001 gtggatggtc gtcaatttat gattagatga atctgtcaca ggaagcacaa cgacaaatgg
    15061 tgctgatttt tcttttactt aatctgaagt ctttatttcc gtaaatcggt aaacagcgtc
    15121 acgaccttgc tcatctttgt ccgatgcact aaaagaagca atgccctctt cctcgtctac
    15181 tgtttttgtc acattttctg ccagtatttt ttcactatca cttttttgtg ctagttttct
    15241 ttgtgcgtct tccacactac ttccttctga ccgcagcttg taaaattcat ccgagacatc
    15301 atatatagcg aaagtaacgc cgttcaaccc ttttactcct tgaaagttat ccgggatatt
    15361 tattccgtcg ttttgactga tacttggcat cttatcgctt tcgaacattc gattatgcaa
    15421 agtaaatgta ataggtgatt ctctagctga cacagtcgga gaacctataa aaaagaccag
    15481 taggataaaa aagagaacag caaatatatc cacacgtaca ttttttgttt ttttcatcgc
    15541 tgttcacctg cttttttctt acgatggata aaataaaaga agccagaaaa aataaagatc
    15601 agtcctaaaa agacaaaggg cagtagacct atcccacctg tcgaaggaag caagagagtc
    15661 ggattgatcg tcaacttagc ggtatctgtt gcgataatcg tataattatt gtttacactt
    15721 ccttcgccta aactaatcgt tactatttgt gatccaatat ctgtccggct gttaactgtt
    15781 agggtataat ccaatgattc gcctaagaca acaccggtaa cgtggagatt tccaaccgaa
    15841 atcgagtgac tttgggcatc ataattaaag gcagcgtcat cgacagtaat cactgcgtta
    15901 gctttataga tggtataagt tcctgatgtg ttggctatat cagtaaatga caacttggga
    15961 tttgctgctt tgactttgtc tattccgcta ttcgttagtt taattgtata gctgccagca
    16021 ttaaccatat tttttgctga ttgtgcatct gctgtgtaat ccatactgct aagtgccact
    16081 tgtactggag taagcgaact agtgacaccc tctgctagct tgacatggat tgacgggaca
    16141 cctgcttttc catcatatgt cttactgcca ctgagctcta ccgcaccgct gatttgctta
    16201 atcgtataga catcagatgt ctgcacgata gtaaataaat agtttgttct ggcagctagt
    16261 ttagctagtc cgctttttgt caattgccaa gacgtatagg ttccaatttc agtaggcgca
    16321 gttacttcaa ctcccttttc attcatcgct ttgaaatcag ttggattcca tgtgatttga
    16381 tcaatactga gatcattgtt taaaagggat gttgattctg ttttatcatt aaaggcatat
    16441 ttaacgatcg gtacaacaga aagaccatca tagtacttac tgccatttgc attgattgat
    16501 actgttgcat ttgtcttata atataggatg acttcagaaa tctgttttcc atttgcgtcc
    16561 tttacgatta catttccatt tgtatctaaa ctagctaaaa cactcattgc tgattggttc
    16621 tttgtttcta tcgctgaaac agctgtaaag gtatatccgt tgatagtagg cgtattagtc
    16681 gtcaggtctt gtgcagcact tccgtaagta agattcatta ttttagaagg agcaatcatc
    16741 tgtccaaaat agtcttgata cttaatagtg attggtgttt gtttgcctgt ataggtcacc
    16801 gtatcgacgt tactttctag ggctgtgaaa gaaccgtttg tttgtgcaaa agaatagccc
    16861 gttactgctg ttggagtgat aaccactttg tcacccacat ggattccaga taatgtagtc
    16921 ccgtacgttc cgatttttgc tgctccacca ttgactgtat aagaaaaagt tcctgtccga
    16981 ttcgcattga cctgaaccgt tgctgcttga taggttggaa aatttgaaac atttccctta
    17041 ccatcaacga tctgcccatt tttgatcgag taccccgtga atggaatatc atttgctaac
    17101 tgtataccga ttgaaaaagt actcatacct cctccggcag gaagccctcc ccatgttgag
    17161 ggtgtaaaag aataactttt atccgcattg ggtgtaactg aatcgatcgt atttccagcg
    17221 gtagccaccc ccatatagat actttttggg aatgtgatag tagtaccatt tactgtggag
    17281 ccaataaaca gattatggat atctggacgg ttatcaataa aagcgattgc tttggttacg
    17341 ccttgtaacg cagttttatc cgcactcatc gatccgttaa ccgcaacaat gactccatct
    17401 ttggcatata tcactgtata tccatctggt atggtatgtc cgttgaaatt cgcagtggcc
    17461 gcaacagtac taaatgtctg gcttctacct gtttctgaat aagctttctc ccctgctgct
    17521 tttgctgctg tgatttcttt ttctgtcgga tcaatcagtt gagtcgagga tgtggttgag
    17581 gttgtctcgt tgttgctaga ctgattggtg ctttctatgg actgtatttc agaatagggt
    17641 ggactatttg tctcctctgt cgtttggtta gcaaaatttg acgtttctgt ataaacgttt
    17701 acataaatac ttgataagct ccctaagaaa gtaaaaatca agatgatcga aatcataatg
    17761 ggatgtaagt attttctttt agatacagct ttgagtctag aaacaaaatg tttttgacat
    17821 atacgttgga ttttattttg agttttctta atcattttca tccctcctct caataaaaaa
    17881 tcatcaaata atcctttaca cgttattcta aatttaattt ttattatttt attgatgtat
    17941 agaaagtttg gggctttttt tctcttttta ttctaaggtt attttagtct tgtcctttaa
    18001 tacaaagatt ttatttttaa ataaaaaata ctcttttttt acgattattg aaaaaaaaag
    18061 ataaaacttg attaattgaa gtattgctta aatttggaag ctatgaggga agaataaaag
    18121 agttaggacg ggattatcta ctatttttct ttgttttaaa tatttgttat ctttgaacat
    18181 aaaagagaaa tgtgtcgact gcagtgttgt gtccctcatc acgaataaac ggtgttcaat
    18241 tagctgatct tgtattcact taaaaaatgg taaagtacga gttgctattc cccattttca
    18301 attttctaac gctcaggaca aaatactgtt ttcacaacct cattggaata aaagcatata
    18361 tattgtttca agaacgaaat cttgttatct ttttaaaggg catctgtcca aaatctaatt
    18421 ttaggaggta cgttttggta tttattcgta caaataaaag agaattatct ttattcatgt
    18481 aacgcatcca attgattttt tcatgtacgc tcctggaacg attttactga cgattgctta
    18541 tttaactgcg aatcgctcat tagcttttat catagctatc catattttga ataacgttct
    18601 tggctttgta ttatagatac agatgacaaa aaaagttcaa gtatgcggtg tagaaccaat
    18661 atcgcatact tgaacttttt tcaatgagta taaattaaaa cttctaattt tcaattccca
    18721 tcatctgata tgcccgaccg tctgaacttc ttttgaaaaa gccaaatgtg attagataac
    18781 gtctttgttc gacataatcg ccactagaaa atacgttcag cagtatcgaa ttaacctctg
    18841 tttctgtata agtcactcca tggtcaaagc gtttcaagac ttctttcaat aggatgtatt
    18901 tcttttttgt tgaacttggt acttgtttta cttttccatc attcatgaat gtgtctaaaa
    18961 ctttcatctt ttcttgctcc gtataagtat aaaccatctt ctttctcctt ttgattctaa
    19021 gaatactcca tttatgttag aatattctgg attttttatt aaagcggtta ctcttaagaa
    19081 agatattatc atagtgctct tttttttact actacttata ggtagattta ttctaatcca
    19141 ttattctccc ctaccaataa ttaaagaaaa aactggatca gggtgttcaa attacatgct
    19201 aagaaaaaag aacccttgaa taccaagggt tctttctcag tctgataaca atgattaacg
    19261 acggatttct ttgatacttg tgaaaagtcg ttaaagtatt gatataaaag gatttttatt
    19321 acctctgact acatattgac taatttgtta aaaaatcgtt tttcaagaat cctgtaatac
    19381 cagtgctttc accgtttttt taactctcta aagaactaaa agcaatcaga ggttttacct
    19441 tttcatttga cgttaagtgg cgataaattt tcgaagttgt ttggtcagta tggcctaagc
    19501 gatctttcaa gacttcccag ctacagtgtt ccgctactaa gcttgccatt gtatgacgag
    19561 ctaaatgagt atgaaatggc ttattaattc ctgagcgatt cacgcaccgt ttgacgtgat
    19621 catttacata ctctcgacga taaggaacac cacgtttact agaatcaaga tacacaaaca
    19681 aatatgtttc ttctataata aacctgtaat gttgcctcat ctcacccaaa gccttaatca
    19741 actgttgtag cttttccata actagaggag ttactagaac tcgtctagca gaactaactg
    19801 tcttagtagt ttcaattcga aaatccttac tcttaccttt aactgccgtt tgcttataga
    19861 acagaatttc atttcttttg aaatctatat ctccaggctg tagagcaagc gcctcgctga
    19921 ctcggcagcc agtgtaaaat agaacatgga aaagtacaga catcggtaat tcctcatgaa
    19981 caatgccata attcaaaaaa gttctcaatt catcgacagt aagaaacttg tcctcaatat
    20041 tatttatggc ttcttttaaa tcagttaccg ttaactgata ttttggcatt ctcacagact
    20101 tcattggatt ttcacttatc aaattctttc tttgacaata tttaaaaatc atatttagaa
    20161 cttgaataag tgattgtaaa ctatttttgg catagccaac atttgttcca ttaacgccac
    20221 attcctttaa ggtaaaaagc aattcttgaa ggcactctgg tgtcaaatca gacaactttt
    20281 tatcaccaat ccatcgaaca ataattttta attgttcctc tcgcttatca taggttattt
    20341 ccttttgttc atttaacgaa cggtaatatt ggaaccattg ctcacctaca acactaaact
    20401 tgtcattgct tgcaacaatt tttttcttaa gcctttgttg tttctccatt tccagataag
    20461 cgagttgcgc ctcacgtttt gaattaaagc cacgtcttgt tgttcgatat gcttgaaagt
    20521 accatgtgat cctaccagta gaactgattt gcttcttgaa tcgatctgtt ttttccattt
    20581 attgattcct cctattttga aggaacccaa caagcagaaa ccctttggaa atctttctat
    20641 ctatgtcatc attgtaccgc atcttagaaa gaaaatgtaa cttataaaga tcgaagtagt
    20701 cgcaattttt ctcgatagtc attcgaatca ctctttctca tccattctaa aacatcatca
    20761 cgaaaatagt gtcctcgaat cccgtaccaa cagggaggaa actcaccaag ttttactaaa
    20821 cggttcagtg tactatcact tatctgaaat agtcgcttta aatcagtatt tgttaatacc
    20881 agaggtaaat gactgactgc aagcaatttt tccgtgtaat cttccagttt tttttgtaaa
    20941 attgaataga actctatttc cagagaatta acatttgttt taacttccaa ctccatcacc
    21001 cttctctctc aaaatcaaaa cgaccaccac ggtacccctt aaaattgtaa aaaaacactc
    21061 cagtactcca tcaaaacgga ctagaaaaga ggaattttta atatgaaaca aggaattgca
    21121 gacataaaaa taattaaaga aattcttgaa aaatctacgg ctaatgcaat agcattcgga
    21181 acaggaatca acttgagtac agtaaaaaaa cttaaaagtg gagagagagc tgaagaaaaa
    21241 ttaaatcttg ccgatgctat taaaatcaca gaatttggca tgaaaaatat gccaaccaaa
    21301 atcgaaatat ggaaatccta gctcttccaa agccatttgt cgttggtacc aaacactgcc
    21361 gagacttttc ccaataatat cgccaatttc acgatcatta agaccgatga agaaggacat
    21421 aaaaatgatt tcttgtcttt gactaggtaa tgctgcaatc gcatttgcca actcctcatt
    21481 ggaaataaag attttcatac caaggacaat aaaagtacca ctactattca aatttggttc
    21541 caaataatag aactctgtac ctaacccttt ttgttccaat atttccattg atatttgccg
    21601 ttggcgaaat ctagcgtact tcttttgaat atttaacgcc tcattgcaga ctactttctt
    21661 acaaaaactg tcaaacgtat gttgaatgtg ggtttcctct gagtcgccca taatattctc
    21721 cccctcaaaa aattcattca actcgtttta cttctatccc tttccattat tactacaatc
    21781 tagga
//
LOCUS       DARBFO010000042        21428 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000042 DARBFO010000000
VERSION     DARBFO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.043"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            109..810
                     /locus_tag="SCK51_001668"
     CDS             109..810
                     /locus_tag="SCK51_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG1252302.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1419..2435)
                     /gene="tsaD"
                     /locus_tag="SCK51_001669"
     CDS             complement(1419..2435)
                     /gene="tsaD"
                     /locus_tag="SCK51_001669"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEG1252303.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2432..2896)
                     /gene="rimI"
                     /locus_tag="SCK51_001670"
     CDS             complement(2432..2896)
                     /gene="rimI"
                     /locus_tag="SCK51_001670"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEG1252304.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2903..3445)
                     /gene="rimI"
                     /locus_tag="SCK51_001671"
     CDS             complement(2903..3445)
                     /gene="rimI"
                     /locus_tag="SCK51_001671"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEG1252305.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3429..4151)
                     /gene="tsaB"
                     /locus_tag="SCK51_001672"
     CDS             complement(3429..4151)
                     /gene="tsaB"
                     /locus_tag="SCK51_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEG1252306.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4342..5322)
                     /locus_tag="SCK51_001673"
     CDS             complement(4342..5322)
                     /locus_tag="SCK51_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1252307.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5346..6830)
                     /gene="galT"
                     /locus_tag="SCK51_001674"
     CDS             complement(5346..6830)
                     /gene="galT"
                     /locus_tag="SCK51_001674"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEG1252308.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6842..7831)
                     /gene="galE"
                     /locus_tag="SCK51_001675"
     CDS             complement(6842..7831)
                     /gene="galE"
                     /locus_tag="SCK51_001675"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEG1252309.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8079..8246
                     /locus_tag="SCK51_001676"
     CDS             8079..8246
                     /locus_tag="SCK51_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEG1252310.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8308..10119)
                     /locus_tag="SCK51_001677"
     CDS             complement(8308..10119)
                     /locus_tag="SCK51_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEG1252311.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10116..10481)
                     /locus_tag="SCK51_001678"
     CDS             complement(10116..10481)
                     /locus_tag="SCK51_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEG1252312.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10644..11039)
                     /locus_tag="SCK51_001679"
     CDS             complement(10644..11039)
                     /locus_tag="SCK51_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG1252313.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11057..12019)
                     /locus_tag="SCK51_001680"
     CDS             complement(11057..12019)
                     /locus_tag="SCK51_001680"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEG1252314.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12019..12231)
                     /locus_tag="SCK51_001681"
     CDS             complement(12019..12231)
                     /locus_tag="SCK51_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEG1252315.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12252..12950)
                     /locus_tag="SCK51_001682"
     CDS             complement(12252..12950)
                     /locus_tag="SCK51_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEG1252316.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12970..13512)
                     /locus_tag="SCK51_001683"
     CDS             complement(12970..13512)
                     /locus_tag="SCK51_001683"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEG1252317.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13644..14648)
                     /locus_tag="SCK51_001684"
     CDS             complement(13644..14648)
                     /locus_tag="SCK51_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEG1252318.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14645..15634)
                     /locus_tag="SCK51_001685"
     CDS             complement(14645..15634)
                     /locus_tag="SCK51_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEG1252319.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15631..16437)
                     /locus_tag="SCK51_001686"
     CDS             complement(15631..16437)
                     /locus_tag="SCK51_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1252320.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16603..17559
                     /locus_tag="SCK51_001687"
     CDS             16603..17559
                     /locus_tag="SCK51_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1252321.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17636..18154)
                     /locus_tag="SCK51_001688"
     CDS             complement(17636..18154)
                     /locus_tag="SCK51_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG1252322.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18242..18391)
                     /gene="rpmG"
                     /locus_tag="SCK51_001689"
     CDS             complement(18242..18391)
                     /gene="rpmG"
                     /locus_tag="SCK51_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG1252323.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18619..19065
                     /locus_tag="SCK51_001690"
     CDS             18619..19065
                     /locus_tag="SCK51_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEG1252324.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19260..21155)
                     /gene="asnB"
                     /locus_tag="SCK51_001691"
     CDS             complement(19260..21155)
                     /gene="asnB"
                     /locus_tag="SCK51_001691"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEG1252325.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6444 a         4549 c         3699 g         6736 t
ORIGIN      
        1 atttgtaaag tattaaaact tgtttctaat gttggtatgt taaaacttta caaattcttt
       61 aaaaaaagtg acaataaata tagtgaacaa taaggagagg actttttaat gcgtgttaat
      121 aaaatcgatt ttcacttttc aaaactcaga tatcaacctg attttgaaca tttctcggat
      181 gaagagttcc aacagatcaa ggaacatact gttcttcgtt cttataaaaa aggacagatc
      241 cttttcgacg atggtgatcc cagacaacgg ttgtatatct tgaccaaagg actcgttcgt
      301 ttagaacgtt atgatgaaag tgcaacttat tactactatg actatgtgaa tacaaataac
      361 ctttttccta tgtcaggaat gtttggagaa gagacctaca cttatacagc tcaagcgatg
      421 actgatattg aaacattcta ccttcctgtt tcctactatg aaaaattagc acgaaaaaat
      481 cccgaacagt tgatctatat gatacaaaaa atttctaaag tagttgaaat acatgaattg
      541 cgtatccaaa tcggattgac ctctagcgcc tttgaccggg taaaacaaaa tttattcatt
      601 ttgaaagaag aattgggcgt accaacaaaa gatggtcata tcaccattcc ttatccgatc
      661 accttaaaag aattagcagt gaatagcggc acgacacgtg aaactgctgg acaagtaatc
      721 aaacaactac gtgattccgg gctgttagat tatgacaaaa agcgttttac tttctgtaag
      781 acagctacta ctccagctat gagagaataa cgcgaacaaa aataagcagt gaccctgata
      841 aatatcaggg tcactgctta ttttcctccc taaaacttct gaaagatccg gcagttttag
      901 atcactggac aagactcaaa gattttctat gtgtgtttca gttatgtaat attcactgtt
      961 tgcaaaaaat actgttagat taactagatg aagcgtcttg tcctcatccg atctagatta
     1021 ttcgatattc ctcgacaata aacgattact cagtgagggc aatcagagga acggataatg
     1081 ataaataagc tgtaaaaaat agcaaaataa caataatgat taaaagtatg taaaaaatag
     1141 ggctttgaca aaaacgtgtc acagccctat ttcgattaaa cggtgtgtac ctgtcaactc
     1201 ttcggtcaca agtcataatg ttcataaaca tgaggagctg ttttttgaaa ttgttccttg
     1261 tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta aacggcgttc aaaagcttgc
     1321 atacgcggta ataagaacag ccaggtcaaa aacatgagaa gccgtttttc ctgggctgtt
     1381 cttattactt gatgcagggc agctttttca caacctcttt attcagataa aaccaagctt
     1441 ggatcagcat tcaactgata gctagcaaaa tttctttttt gcatttccac atgtgctgct
     1501 gctccaatca ttgcagcatt atctccacaa agtcgaagag ggggaatcac tagttctacc
     1561 tcagggagtt tagctgccaa agcatcctgc aatccttcac gcaacccttg attggccgct
     1621 acacctccag ctataatcag ttgcttgact ggataatttt gacaagctcg aagtgtttta
     1681 gtaatcaata catcgatcac acttgcttga aagctagctg ctaaatcatt tttgtctaga
     1741 tcttctccac gctgttgtgc attatgcacc aaattgataa atgcactttt taatccgcta
     1801 aaactgaaat cataattatc ttcatggatc atcgctcgcg gaaaatggta attatctttt
     1861 ccctgatgag ctagctgatc gatttctttt ccgcttgggt aagaaagacc taatactctt
     1921 ccaactttgt cataagcttc tcctgctgcg tcatctcttg tctccccaat gatttcatat
     1981 gatccatctt cttgcatata gactaattct gtatgtcctc cgctaacaag aagtgccatc
     2041 aatggaaact ggaatggttt aaccagacgg gctgcataga tatgccctgc catatggttt
     2101 acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct tagctgcaga aataccaatc
     2161 agcaacgagc cgacaagtcc tggtccataa gtgacagcga ctgccgataa atcttctgcc
     2221 gacacaccag cttctaccag tgcatcttcc aagcataagg tgatttgttc tacgtgatgg
     2281 cggctagcta cttcaggtac tacgccacca aaacgtttat gactgtttat ttgagaagca
     2341 acgatgttag ataagatctc tgttccattt ctaacgaccg ctacactcgt ttcatcacaa
     2401 cttgattcaa tcgccagaat caattcttca ttcatttcat cgtctccgtt tttaactttc
     2461 tgctcataat gatcgcatct tccgtcggat tgcgataata tgatttccgt ttacctaata
     2521 tacgaaactt ttctgattca taaagttttc ttgctgcttc attagataga cgtacttcta
     2581 aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag taattgtaac aaatgccgtg
     2641 catgcccttt tccttgttct gaaactgcaa cagcaatatt tgtgatctcg acttcatcta
     2701 ttactttcga gtatccaata aagccacaga tctggttatc ttcagctaaa agcagataat
     2761 cagagtgagg ctgttggatg tctgccaaaa actgcatttt cgtccaaggt gaaccatgtg
     2821 gataagcatt ttcgctaatc atccatacgc attctgccaa gtacgcttca tcaaaatctt
     2881 tttttctgca aatcaactaa tctcactcat tttctaaaag ttttatcata tccaacgcat
     2941 cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt tgcttgaaac cccaatttcc
     3001 gataaagtct ttgtgcatct tgattactca tacgcacttc taatgacatc gtctcacagc
     3061 gattcattat agcaaagttc tcgatttcgt caataagcag cgaaccgatt cgtttacgct
     3121 gatatgccgg tcgaacggca atattcgtga tatggcaatc taaaccgaac gctcttgaac
     3181 cgataaaacc aatcattctt ccattatcaa agatacctag atatagatga ggaagcggtg
     3241 acttcaattc tgatataaag gcactttttg tccatggcaa ttcgccatta tagacttctc
     3301 gttcgacatc cagcagctct ttgatatctt ctgtagtcaa ttctcttagt acgtaacgac
     3361 gatcgatttg ttcgactgtt ttttctgtat aaatcatttt atcttgaaaa aagcttctgg
     3421 catacgctct aaattttttc aacataactt tcgtctccag gtgtgtgcgt ctccaaccat
     3481 ttttcttctg cttctactcg ttttaaatag cgaggaagaa aagcttgttt ctctgcaact
     3541 ggttcaacta aagcacctaa ttgagccaca gttacaccac ttggaatatc ccatgctgga
     3601 actgtattaa tcatcgcact aggcaatatt tcctttattt cagagaagaa tttgtgacaa
     3661 tcactcccga cgaaataaat agcttcattc agcttttgta gttgtgtgaa caaatcagaa
     3721 gcagaaatat gtacatcttc aagtacagtt tctaatctat tttctttcca gcggtatgcc
     3781 ccagcgtata cattttttct tcgagcatca aacatgggca caataatatt atccacaccg
     3841 atgcaattag ccgcgatggt tttcagacta gatactccta ctagctcttt atctaatgta
     3901 taagcaatcg tttttgctgt cgttacacct atacgaagcc ctgtataaga accgggacca
     3961 tcagaaaccg cgattcggtc aatgtctttt ggacttattt gaagatctgc aaacaattgg
     4021 tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga tatttgtttg tatttgtcca
     4081 agaatttgtt cgtcttctac cacaccgatc gccaaagttt ggtttgcagt atctattcct
     4141 aatgtaatca tatatttgat cctttctgct atgacattct cgccttccat tgtagcatac
     4201 ttttttattc tgaggatgaa aataccaaag gcaaaactag tacaataata catgattatc
     4261 tgctggcggg caaattttgc cccttcctga aacacaaaaa agctgcggta tcactccatt
     4321 ttcccacagc ttttccatct tttatttcgt tgattctcga ataatcaact ctgtttccac
     4381 aataatcttt cttgaaaccg gtgctttgga attacttaat tgaagaatcg tctccactgc
     4441 tagctctccc atccattcgg tataaacttt tacagtagaa agcgagggcg ttacatattt
     4501 cgctacactg acatcattga agcccatcac agaaatatct tctggtacca ctaaaccttt
     4561 ttcttgaatc gctttcagtg cgccaatcgc taatgcatca ttagctgcaa aaagcgcatc
     4621 aggaacatgc ggattcgttt ttaaatattg cttcactgct tgatatcctg actcgacaga
     4681 aaaatctgct tcgataattg gaccttcgtc gtagcctttt tctgccaaaa cttgttgaaa
     4741 tgccataatt ctgggatctt ttagccgctg atggtttttc ttcgtatatt ctgttccagc
     4801 aagcatagcg atatgttgcg ctttatggga gacaaagtgt tcaatgactt tttgaacact
     4861 tgacttaaag tcgacaacta gagagtcgat tccttgagaa gtgccgtcag aatctaccaa
     4921 aagcaaggca ttttgggatc ctttcatttc taataactga gcttcgtcga attttcctag
     4981 tgcgatcgtt ccatctgccg gaagatcact aatggaatcc cacgtttctt tcaataaagt
     5041 aacgtttgct tcttctgcct ttttttcgat tcctaggcga atagataaat aataaagatc
     5101 ttcaagctct tccgtatcgt cgtaccactg gatcaggcga agcgtttgct tagtatttgc
     5161 tgcattttta cgatgtttcg tataatttaa gcgttctgct gcttcaaaaa ttttttgctt
     5221 tgtggctaag ccaacggaaa gatcaggatc atagttgaga actcttgaaa cagtagcagg
     5281 agaaactcct gccaactgcg caatgtcttt aattgttgcc atttgtctct cctccttttt
     5341 tacgtttata aaatcgcagt aaatcgttca aacgctgcct gtcctttttc atctcgcttg
     5401 aatactccag cgtcttccag tacacgtgca aaaacctgac ctacagattt ttgtacgaca
     5461 tcggtgaccg tatctttcgt caaatcagga tgttcttttt ttaattgttc tgcccacgga
     5521 cgatgatatt cagccatctg gttttcttct cctaataaga aatgctctac ttctactagt
     5581 tctggtttca aacgaggtgg taaaacagca agccccatta cttcgatcaa cccaatgttc
     5641 tcctttttga tatgttggac atctttatgt ggatgaaaaa tcccatcagg atgttcttct
     5701 gaaacattgt tgtctcgaag gacaaggtcc aactcaaagt aattgtccct tcttcgtgca
     5761 attggcgtaa tcgtatggtg tggtgtacca tctgctgaaa aagctctgat agatactgtt
     5821 tcatccgaat atgctttcca tttttccaat actgagttag ctgcttcaat aagcaagttt
     5881 ttctctgttg attgcaaacg gatcacagac atcggccatt tcacgactcc tgcgatgact
     5941 tgcggataat cttctagttt gataagtttt tctatcggtg cttttgccat aggaaattca
     6001 tgacgtcctg cctgataatg atcatgagaa agaatcgatc ctccaacaat tggtaagtca
     6061 gcattcgaac caacaaagta atgaggcaag acttcagtaa tccgaagcag tctagcaaat
     6121 gtttcctttg tgattttcat cgggcggtgt tgttctgata aaatgatcga atgttcattg
     6181 taataagcat aaggggaata ttggaatccc cagctttcac cgtctagatt cattcgaata
     6241 atccggtgat tggtgcgggc tggatagttc aagcgacctc tatatccttc gttttccatg
     6301 caaagcatgc aggtaggata gtctactttt accgcctcgc gttctgctgc gatttgtttt
     6361 ggatcttttt ctggcttaga taaattgatt gtgatttcca gttcaccata ctccgactct
     6421 gccggaaatt tgatgttttt tgcgatggct cttgttttga tgtagtcatt tttcttactt
     6481 aaattaaaga aatactcagt tgcttcctct gggcttttcg aatagtgctg tgcaaagaat
     6541 gcattgacga ctgaaggtgg cggtgtgaga aaatccatta attgcgcttc aaaaatctct
     6601 tgttctgctg gcaattctga aagaacacca ttttctcttg ccttagcgac aagctgatcc
     6661 aataagtcta cagattcttc cttaactggt cttacttcaa tgctttctag agcatcttct
     6721 cctaccatcc ctaaaagacg attctgtaaa tataagcggt ccatttccat ccatccgcca
     6781 gcttcgatcg caagtgtggc aaaatcacaa atcgtttgac tgattgacat atctcattcc
     6841 cctattcttc ataaccattt ggatggctga catgccaatc ccatgctgtt ttgataatcg
     6901 ctttgatatc tgtgtattca ggtttccagc ccagaatctc tctcgctttt tcacttgaag
     6961 cgactaatcg gctaggatct ccagctcttc ttggtgctat ttctgctgga atttcttttc
     7021 ctgtcacttc tctagctgct tctagcattt ccttgactga gtatcctttg ctgctgccta
     7081 aattgaagaa gttgctttca tttccttctt tcaagtattc caaagctaaa atatgtgcag
     7141 caatcaaatc ttctacttga acataatctc tgatacatgt tccatctggt gtatcataat
     7201 cgtctccata aacagccaag gcttttcttt gaccaagagc tacttgtaaa atgattggga
     7261 caagatgcgt ttctggtgta tgatcttctc cgatagatgc atctgctttt gctccggcta
     7321 cattgaagta gcgcaaagcg acatagcgca tgccatatgc ttggtcacac cacttcatca
     7381 ttttttccat catcaattta ctttcaccat acggattttt aggattagct ggtgtatctt
     7441 ctgtgattgg agactctttc ggctctccat aagttgctgc agtagaagag aatacgattt
     7501 tattcacgtt gaactcgtgc atgacttcaa gtaagatttg cataccgtat acgttgttgt
     7561 tgaaatacat caatggtttt tcgacagatt ctcctactaa agagcttgca gcaaaatgga
     7621 ttactccttc aatcggttcc ttttcaaata cggaacgtag aaattcttta tcccggatgt
     7681 ctccttcata aaaatgagca tctggatgta ctgcttgttt gtgacctgtg agcaaattat
     7741 ctaccacagc tacttgatac cccttttgaa ctagctggtc tacagcgtgc gaaccgatat
     7801 aaccggctcc gcctagtact aaaattgtca taaaaaacac tcctttgatt agtaaaattg
     7861 tgtttgttga ttctatataa tttaaaaaac gaagtttact tttatggtga aaacaaacaa
     7921 agtaaaattt cgtaaattca tatggtaaat ttataataaa acatttagta aacattttca
     7981 agggtttttt gcaaaggttt acaaacaaac gtaaaactag cctttttttc tcttttgttc
     8041 cataatagat ataacaaata ataaatgagg tggattcaat gaaaaaattt gtatctggaa
     8101 tattagtagg aagtctggca acagccgctg ccgttgctgg tttggttgct tctgtgaaaa
     8161 aaacagtgat cgatccaatt gacgaaaaag aagcaatgat cgaagaaaac cgtaaaaaag
     8221 caatgcgtaa acgcgtttct cgttaataat tatttaaaaa tacgcatgaa gactttctaa
     8281 aaaacagata agtcatcatg cgtattttta tttgtcagtc aatataagtt gtgctatttt
     8341 atctgcctgt ctcagattcc atggatctgt ataaggtcta gctactgctg cagttagcca
     8401 atccacacct tcaacaggta ttccatagca ataaaagtca gactggattt cccctgaagc
     8461 atcaatcacg cgattcgtac ctcgctcaac atctatagct tctgaatgat aacttcctgt
     8521 attatctgaa aatgaaaaag ggcgaacaag tttgttttcc ttcaaatttt ttatgacagg
     8581 attacttgaa cgagaaaagc ttgtttgtgg caatcgagct tcaatcaaat agcgactgct
     8641 gatttcttta tcttgtagag aaataacata gcggttgtgc ttttgctgaa tatcaaatgg
     8701 cggttcgatc aaatgaaaaa tacccgcatc tatcagtgct gctaattctt tcgtccgaat
     8761 gattggcgga ccgatcgtta aaaagctatt tagatgagta aaccatcccc atagtatttc
     8821 tttgtattcc ttagcagaaa aaacgtgcca ttcaatagcc tgccggattg gatcacgcca
     8881 gtcttttaat gcatcaaatg ttgaagcaaa tgctcctgtg caattgcctt tttccgcttc
     8941 ttttatctgc ccttcaataa atattcggct agcttctgaa aagggctcat tctgataggc
     9001 aagaggagtt tctaaatacg accaatccca tctataaggg attagtgctg gatgctttcc
     9061 gagacactcc tctgaagaga gggagagaaa atcatgctca aaatttttct ttggaatcgg
     9121 caagtgatat tcttcgatga ttttcttgta ataaaacaac tcaacgtctt ttttcagata
     9181 gtcaaaaaat gtttcgccag tcaaaaatcc cgactgatgc cattgctcca ggttttcttt
     9241 tgttagtaac cgtggccatg ccattgctcc gccttgcttt tgatttcttc ctcgagggta
     9301 ataagggagc ccccttcttg atccactgta tacaatcggt tccttgcctg atggatgata
     9361 gaccagtttt cccttttcgt tctcgaagcg ccctcctctt gatactgtaa acaatccgac
     9421 ataatcaaaa aaacttaagc ctagacctct taaaaagaca ttttctttag gcggtatctt
     9481 cgaaagatct acatctgcag gattagaagg aagctgatac agtaaaacat ttttctttgc
     9541 gtagtctacc cacactcttt cttcttctgt ctcatcattt tcccagtgac cgcttgctaa
     9601 aacaagctga tcgatagaat agctttgctg atctgtctgt aaaagataac catcttttct
     9661 tttgcttact ttttttacta aagaataaac aaactgaata gaagctggaa attcttcttg
     9721 caactttgag aaaaaccagc gttgatagag tccgtataaa catcgagtag attgctgatc
     9781 tttttccaga cgattgattt cttctataat tagttgctta tttttggtta ccgtctgttc
     9841 gatataatct gtagcaggtc ctttactcca ttgaaacaag ttgggtccct gtgttaccaa
     9901 tcctccactg gacaatgttt catctgtaaa aagcgtcact tgttgagaaa cagaattcat
     9961 taataactcc ggtggctgat tcaaccgcca aactcgacct ccaggaccat tgggatcgaa
    10021 aagaaaaagt tcaagagaat gtctgtcctg attattatgg agaatccttt ctgctacact
    10081 taatcctcta ggtccggcac caatgatccc tattttcatt tgcatgcctc catttcgata
    10141 aattttttca ataaaacagc atggttctga tgttcatttt ttgcaccata aacaaaagta
    10201 atgactggat gttctttcat aagttgactg agtttaaaaa tagcttgctt cttttcttct
    10261 tcgaaaatca attcttgctg gtatttcttt gcaaatgaag caaatcgttc tggtacatgg
    10321 tcaaaagttt tgcgtgcttc attactaggt gcaatttctt tcaaccacag atctaactgc
    10381 gctttttctt ttgaaattcc tcgtggccag aatctatcca cgagtattct atagccatcc
    10441 tctttttcag catcaaggta aacacgtttt aatttgatca tagttgtttc cttctttcta
    10501 ccttttcgct tagcttatat atgtagataa tagcacaaaa taaaaaaaat gaacggcaca
    10561 actatccatt gcgccgttca tgtgatcttg gtcaaaatct aatgattgat tcggttttta
    10621 aagctgtcat acaatcatct gttttatact tttttacttt tgatttttcc agaccatccg
    10681 tcatatccac ccttaagtaa ataaatctct tgataacctg ctttacgtaa tttatttgct
    10741 gtacggatgc tcaaactttt cttttgatcg taaatataga caggctggtc ttttcgaata
    10801 gatcctaatg aatctttcaa aacagtgtaa gggatgtttc ttgcacctaa aatatgccca
    10861 gcatcaaaca catctttttc gcgaacatcg attacttgtg cttttctcat tccttctcga
    10921 aattcttctt ctgtcaaagt cgttgctgaa cgtttggcca ttactcgtag atagacttcc
    10981 caaaggatga ttgcaagcac aatcaatagc aaaatgatat taattaccca taaaaccatg
    11041 taatgcctcc gtttttttaa gcaaatttca aggctaatga ggcagcacca atgacaccag
    11101 cttcattacc aagctctgct aatttgattt ttgttgattc tcttacttgc gggaaagtaa
    11161 attctttaaa atatttctct acacggctac gtaaaaattc tcctgctgcc gatactcctc
    11221 cacctaacac gatacttgac ggatttaatg tattccccaa gtttccgcaa gctaaaccta
    11281 agtagaagca tacgcgatca acaaccatca atgcaaacgg atcgtcttct tgagccattt
    11341 caaagatatc tttacttgtt acttcttctc cgttgtctag cattgctttc agttttgaat
    11401 cccctgcgta ttcttctgcc aagaaacgac ctaggcgtac cacacctgta gcgctagata
    11461 cagtttctaa acatccgcgt ttaccgcaag tacattcgaa cccttcagga tccacagtga
    11521 tatgtccgat ttctcctcca gctccgctca cgccgtgaat caaatttccg ccagcaacga
    11581 tacctccgcc aacacctgtt cctagagtaa cgaatacaac gtctggatcg ttttctccag
    11641 cacctttcca tcgttcacct aaagcagcaa cattcgcgtc attatcgata gcgaatttga
    11701 ttcctgttcc tgcctcgatc agttttttca caaattgaac ttctgtccag tttaagttat
    11761 acgcaccaat aaccgttcca gcttcgctgt ccacgcttcc tggggtaccc atgccgattc
    11821 cgataaagtc ttcggctttc atattatata aattcaaacg atgattgatt gattcaatga
    11881 tttcaggaac gatatgctta ccatcatcca aaatattggt atcgatgctc catttttgct
    11941 gaatctctcc ttctggtgtt aaaattgcaa attttgctgt tgttccacct aaatcaatcc
    12001 caataatctt cttgtccatt acaaatttcc tttctttcgg ctttcttcaa ttcggtgctc
    12061 tctttttaat atgcgccacg ctgtcatgta tgtatcatga tcaatcaaac ggctatcata
    12121 cagattacgc aattcgatag tcattaattc aatgtcatag atgcgtgcac ccacatagat
    12181 aaagatgcca aattttttga acagctgctg tacatcatat aagctctcca tctttaagca
    12241 cttccttcat attatacaag taaaccatat ttttttaaac ccagtaaaat acatattaca
    12301 aaaagaaata caaaaactag tgtagaaaga atcctttggt gtatcgaata gcttgtctgt
    12361 tgtttcggta gacccaaaac attccctaac actaatccgc caattagccc accgacatgt
    12421 ccccaaacat ctacagaggt atccagaaga ccaaaaacaa atgtcaacac aataaataaa
    12481 agaaaatgtc tgactagctg ctggatcgct gggttatgtt taaaatgaaa ccctaagata
    12541 aacaacgcac cgaacaaacc gaatatcgaa gtacttgcac caccagacag aacattcgct
    12601 tcgttaaaag caaagcttgc tgtatttccc attatcccgc tgaacatata aatcaagaaa
    12661 aaacgccaat ggccatagat tgcctcgatt tgctgcccca taaaatacag tatcagtgag
    12721 ttcattacga aatgcatcag tccaaaatgg atgaagattg gtgtcactaa acgccaccat
    12781 tcattgaagt gaacaagata aggaccatac attccggcat aataagggat ctcaagtcgc
    12841 ggaaataatt ccatcatgag gaagaccgct gtctgtatcc ctaaaaaagc ataggtccat
    12901 aacggtcgtc ttaaccatgc acgcaatttt atttgctgtt gataattcat gaaaacctcc
    12961 catttcattt tagtcaataa acaattgttg gatcttctga tcaaagctct ccggttgcca
    13021 ttgttcattc aattgttcac gaaaaacaag gctacatgtt tttttatgaa aattatttaa
    13081 ataccggtcg taataaccac cgccaaaacc gattcgataa ccggctctat tgtagataat
    13141 acctggtaca atgaggagat cgatcatctc cggcaaatag agcttctctg attgtggctc
    13201 aagaacacca aaactcgaac gtacaagaac ggactgttca tccatttcat aaaacgccat
    13261 ctgacgtttc ggcaaagttt taggtacaac tattttcttt tgttcttttt ctgctcgttg
    13321 gataaccggc tgtgtatcca gttcaaattc attcgataaa gtcgtcccta tcacttttgc
    13381 tgacttccag tactcgctgg cgaatagctg tttatagatt cgctgttctt tttccttttt
    13441 cttgccactt ttggccagat ttttcagctt ttcgattcct ttccggcgta atcttacttt
    13501 caattggtcc aaaaactcac ccttccttct ctcataaaat ggtataggaa aaaaagaaaa
    13561 atagcaatcg aactttcaaa taaatgcata aatagaataa aaagaagacc tagaaatgat
    13621 tggccttcct tttttaatga atttcaactg ttcagcgcgt gtttttgtct gataaaaagt
    13681 gcatacagaa aatatggcgc accaaaaagg gcaatcaaaa ttcctgtagg tacttcccca
    13741 cttggtagaa tcactctagc aaccatatct gcactaagca gaaaaatgga accgaacaaa
    13801 ccgctcagaa caaaggtcca gttattttct ttacgtacga ctagcttcgc agcatgagga
    13861 gctagtagtc ctacaaaaga taaactacct gtcaccgcta cactaccgct tgccaaagca
    13921 acagctaaca acaaaaggat tttttgactt ttagccacgt taaggcctaa tccttgcgcg
    13981 cgttcttgac caaatgatag caatacaatc ctctgtgcat aaagtcctcc tagcggaatt
    14041 aagataaccg cccatggaag caatgcccaa acataaggcc agctcgcacc ccagattgat
    14101 cccgctaacc atgcattcac aaatccataa ttttccttag ataattggat cgtgcatagc
    14161 aatgttaatg cagaaaggcc ggcattaacg gcaataccgg acaataagat tcgattcata
    14221 cccaaaaagc catttcgatc ataggccgca agatagacac agcaagcagc taaaacacca
    14281 cctagaaagg ctacaactag caatagaaac ggcagcgaat agtgcgaaaa gaaacctaaa
    14341 taaagcatca ctaaaaatcc tgctccggca ttaatgccca atatactaga atctgccaat
    14401 tcatttcttg ttactccttg caagatataa ccagaaacgc caagacaagc tcctgctaat
    14461 aaactgatca gtaatcgagg aactcgaaat tctctaacaa tcagcgccaa agaagatgat
    14521 tctttcccta tgaacgtggc aaaaagctga tccaggtaaa taaaaggcgt accaatcatt
    14581 aaactaccta tagacaggat gacaagtaca aaaagcaaga aatagaacgt ccaatatatt
    14641 tttttcaccg ttgttccctc cttacttgta tcagtaaaaa cggaacgcca atcaatgcaa
    14701 tgactaatcc aaaaggtgtt tcaaaagggg gattgatcat cctcgctgta aaatccgcca
    14761 acataaccaa tccaccgcca actaaaaaag agacaggtag aacataacga tagtccgttc
    14821 caaccattcc gcgaacaact ggcggaacca acagcccaat aaatgaaaca ggtccgacta
    14881 acgaaacggc aatggctgct aacactgcaa ctagacagaa aacaatcaac cgcgttcgtt
    14941 taggatgact tccaagagct aatgcatgtg tttcactcaa atgaatcaaa gatattgctt
    15001 ttcctaatga aaaagaaacc aaaacagcaa gtatatataa aggaaacaat actgctaatt
    15061 gtttccatgt gatattggca gctccgccta caaaccaaaa agcaagatcc tgctgcagat
    15121 taaagaattg ggtaagtgct tggctgatag cagtaaaaaa agaactaata gctacgccag
    15181 ctaaaatcag ctgtaaagga ttcatgccca ttttcgaaaa ctgcaaaaaa ccaaatacga
    15241 gcaaagttac agcaaaagaa cctgccaatg acattagaaa cacagaaaaa tcagaagctt
    15301 taggccaaaa agcaaaacta agggccattg ccaacgaagc acccgcgttg attcctagta
    15361 agcctgaatc tgctaaagga tttcttgtca ccccttgcat caatgcaccg ctcaatgcaa
    15421 aacaactccc tacaagaaat gctcccgcca cacgaggcaa acgaatattt cgtatgattt
    15481 ggtgactttg ttttgatcta tcaaaatcaa acaacgcctg ccaaaaatct gctttttcca
    15541 cagtcgctgc gccatttgaa agagcaacaa aaaaaataat caaaagaaca aaacagacca
    15601 aaagcaggag tctatttttc ttcatagtcc tcataattga tcctcctgct cttttggcaa
    15661 caaatcatag gttagaaaaa ctggtttatt tgttcttgga agcgtgacaa tcgttgcatc
    15721 aatctcaaat atttttttca tacactcttt tgtgatcact tcttcaggtg taccttctgt
    15781 gatcaactta ccgtttttca ttgctaataa ataatctgaa aaacgtgaag catgattaat
    15841 gtcatgaatc gacatcaaga tcgttgtttg tttcatttga ttgatttcag tcaataactg
    15901 taggatctct aattggtgtg ccggatctaa aaatgttgtc ggttcatcca agatcaagat
    15961 atctgtttcc tgagcaagcg ccatagcgat ccagacacgc tgggtttgtc caccagacag
    16021 cgttgcgata tcccgcatac gaaatccagc tagacctgta gcatccaacg cccactcaat
    16081 tgcttcatga tcttgcgtgt ccatgccact gaacagtttt tgataagggt atcgtccgta
    16141 agatactact tcttctactg tcatacctgt agggtgttca ctagtttgtg ctaataatgc
    16201 aatttttttt gcaagttctt ttgaactgta tgtagagata tcagagttat tgacaatgat
    16261 ctttcctgat ttcacaggta gtatacgggt aaaacttttc aataaagtag attttccact
    16321 tccatttggc ccaatcaaac tagtgatttt cccttcagga atcgtaacag acatgttttc
    16381 aatgattttt ttgttttcgt agccggctgc aatgtcagct gctcttaatc tcgtcatttc
    16441 acccatcctt tcgctgaaaa tcattctcaa ttatgacgct gtttcaacat ttcgtcaagc
    16501 gctactttca tatctatagc caaatgaagc ggttgacagc gttcgaaata tcaggaataa
    16561 taaccaatga gaaagattct caattatgtt aggaggaaag aaatgaaatt aaaaacaaaa
    16621 gtattcacat tatctagcat attgctgctg gctggagtac tgagcgcttg ctccccctct
    16681 tcagataaag aaacagctac aactgccaat tcgacgactt catccaatag tactcataca
    16741 gtcacagata cacttggaca taaagtagag attccaaacg cgcccaaacg gatcatcggc
    16801 agttacttag aagactatct gattgcacta ggggaaaaac ctatcgccca gtggactgtt
    16861 ggcagtggtt ccattcaaga ttatctgcaa gatgatttaa aggatgtccc aacgatttct
    16921 tatgaccttc cttatgagaa agtgttaagc tttgagcctg atttgctgtt gatttcttct
    16981 tccgctactg tcgaaggcgg gaaatacgaa caatatagca aaatcgcgcc aacttatgtt
    17041 gtaaaaaacg gaacagatgt cacatgggaa gaacaactaa aagatgtcgg caaagctttg
    17101 gcaaaagaaa aagaggcaga aaaaatcatt tccgattatc aaaaatccat caagaaaact
    17161 cgagaagaac tagcagataa aatcgagaac aagacagctg cagtgttgtg ggtgaccaac
    17221 aactcagcct ttatggtcag caaaaatcgg tcaagtggaa gaatcgtata cgacgatctg
    17281 gaatttggtg ttcctgatct agtggaagag gtctccaaaa aagccacttc tgactggtct
    17341 gctgtttcct ctgaaaagct agctgaatta gatgctgact acatcatcct tgtgaacagt
    17401 gacaaagatg ctgccatgtt caatgaacca agctggaaga atctaaaagc agtgacagac
    17461 aatcatgtac tgcaattcgg acctgaatct agttggctat ataacggacc tattgccagt
    17521 caggaaatgg tcaatgatat caaagaaaaa ctaaattgat tttaactaaa aaaagcatct
    17581 tcacgattag tcgtgagatg ctttttttct tattaacaaa ctagacgcat agattttatt
    17641 cttcttttga agcaacccta ttttttactc tttcccattt ttgaaaacga tgggcatata
    17701 gattttcttg atccgttatc ccctcatact cctctataag ggcgaagtct gaaaaatcaa
    17761 tattcccaat atacgtatcg ccttcaaacg tgctatcgat caatgttcgc cagatccgac
    17821 cagtgtaagg aagaagttct tgaaaaatcc gactaccacc cgaaacatag atatcttctg
    17881 agatcgtttg actatacgac aaaagttcat cgatcgaatg cattacttcc gcattttctt
    17941 tcgtgcgaaa atttggttgc gttgtcaaca cgatgatatg gcgataagga agtgaaagat
    18001 cccccattcc ttcataggtc tttcttccca tcactaaaat tcgatccatt gtatgttcgc
    18061 gaaagaaacg catatcattt ggcaatcgcc atggtaacaa accatttttg ccgatcagac
    18121 catttttatc ttgtgcccac atggaaataa acatattatc cgctccttat gattttagat
    18181 gttttgagat aaaaaaagtc tcaattcttt gaccagcaag gaattgagaa ctttaaaaac
    18241 attattttgt ttcacggtgc aaagtaactt tgcgttcgcg tgggcaatat ttttgttttt
    18301 ccaaacgatc aggattgtta cgtttgtttt tgcttgttag atagttacgt tctttacaag
    18361 aagtacactc taaagtgatg tttacgcgca tgtttttccc tcccattttt acaagaatcc
    18421 tctaagatga ctctcagact tgattatctt accatgattt tttctagatt gctagtgttt
    18481 ttcacgattt tgttaagtaa aactacttga cgataaatac aacggtttta cttctcaaac
    18541 aaaatctttt tctttttgta taaataccct tacacttgtt tatatagatt atgtttttta
    18601 ataaaggggg atactaatat gccatggaac atgaaggact ttcctgcatc aatgaaaaac
    18661 ttagataaat tgacaagaaa aaaagcaatc gacatcgcaa atgcattatt agatgaaggg
    18721 taccctgata accgtgcgat cccaattgcc attgatcaag caaaaaaatg gtatgaagat
    18781 gctagtgaat cagaaaaacg aactttcgaa aaagaaaaaa atccaagcaa gacggatgaa
    18841 cacgatacga atcctcgtgc tggaaaactg cttgattcag atgtgatcgt agaatatgag
    18901 gaagaacaat ggatcgtgaa atctaaaggg gcaaaaaagg caagcaacca ttttgataca
    18961 aaaaaagaag caatcgaaaa aggaaagcaa gtagcacaaa acaaagaatc aactttgttc
    19021 atttataaaa aagatgggac aaaagaaaaa gaaatcgatt attgaacata tgtaaagggg
    19081 ttgtgacgaa aatcgtgtca caaccccctt atgttcgaaa caatggtgtt cctctgccaa
    19141 ctccgaacca taagtcataa tatctataaa acgggaagag ctgttttttg aaattgtttc
    19201 ttatcactca gtgttaaacg gtaggagaaa acctcttttt tattgatatt tgattttcat
    19261 caaacagtca catagtcgat tgcttctgct tgcggaatcg tttcatctac aaagtagact
    19321 ttataccatg tcaagaaact gaagattgtc cagatttttc tttgctcttc tttacgtcct
    19381 tcgtgatggt catctagcaa cttcatgatt ttttcttgat caaaaaattc ttttgcaaaa
    19441 tctgcttcga ataatgcttt gacttgttcg tagccgtgtt cttctttcaa ccaagctttg
    19501 attggcacag ggaatcctaa tttcacacga ttcgaccatt cttccggaag atgtttgttc
    19561 gcagcttgtc tgaaaatatc ttttgtgttg ttttgattca ataaaaactt acttggtatt
    19621 tgttgagcca atttcatgac ttcgatatcc aataaaggta ctcggacttc caaagaactt
    19681 gccatagaaa gtttatctgc ctttaacaag atatcttttg gcatccattg atgaagatcg
    19741 acatattgca tcttattgac ttcatctttg attccttctg ttttctcgta atgaacagtc
    19801 atgatttcat ccacagatgg tgctgtttga tattttggtg ttaacacttc tgatgcttct
    19861 gattcttcga acactcgagc atgaccgata aaatattctt ttgcaggagc aagtgattca
    19921 tataaatgga tccgtccatg gaagtttttc atttttccga ttttacgtcc taaattatag
    19981 cgtgttcctt ttggtaattt tttcaatcct tgagcaatca cacggatgaa ttttgaattc
    20041 gtatggaatc catacgtttg gtatcccgca aacagttcat ccgccccttc tccagattgg
    20101 actacccgca cactttgtga agcaagattt gctaaaaagt acaaaggtac gcaagaagga
    20161 tttgaatcag gttcatctaa gtgatactgg atcaaaggga atgctttgaa cgacatgtct
    20221 ccgtcgatca ctgctgctgt attatctaag tccaatagtt cggttagttt tctggcttca
    20281 atcgcttcat tataggtttt atcatcaaac ccgattgaga aagaatgatc tggtttcaat
    20341 acagaggtta cataactgga atcaaccccg ctggataaga aagaaccaac ttctacatca
    20401 ctgaccgtat gtgcttcaat agaagcttga acggtttcgt cgattttttc gatccattct
    20461 tgtcgggaat gcgtttcttt tgtttcaaaa tctgcatccc aatattgctg aatgtctatt
    20521 tttccatctt tatacgtata ataatggcct tcaggcaaac gatagacatc tttgaagaat
    20581 gtttcgccgt ttaatggaga atattggaaa gtcatataag gtttcaacgc ttctttgttc
    20641 aattctttgt taaaatcagg atgaggcaga aaacttttga tttctgaacc aaacataaat
    20701 gttccgttca tttctgcata ataatatggt ttgatcccaa agtggtctct tgcaccgaat
    20761 aactcattct tttcattatc ccaaatagca aaagcaaaca ttccgcggat cttttgcaat
    20821 aattctgttc cccattcttc gtaaccatgc aaaaggactt ctgtatctgc atgtgtttga
    20881 aaaacgtggc cagctgcaat caattcttct ctcaaaggct ggtaattata gatttcgcca
    20941 ttaaaaataa tgatctttgt ccgatcttca ttgtaaatag gctgagtacc gccctctaag
    21001 tcaataatac ttaatcttct gaatcctaaa gcaacatgtt tatcgatata ttctcctgaa
    21061 ctattaggtc cacggtgaac gattcgatcc atcattgtat taataattgt ttttttgttg
    21121 tccttgtcgt taacaaaacc tacaataccg cacatttaac tttcacatcc taatataaaa
    21181 ttactgttta tttcattcat atgcatctta tcatagaata aatagcggac gaatattttc
    21241 tctcttacaa tttcgtcacg tgaaaacaaa agatgaaagt ttctgttttt acagaaaata
    21301 aagtcccaat atctaattta ccatgttttc ttttttgagg ctaccttatt ctcttcgtgt
    21361 taaaaaaata ttagtggatg ctatttccaa aaaaaccagc aaaaaaagac acgacctaaa
    21421 gatcgtgt
//
LOCUS       DARBFO010000043        20174 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000043 DARBFO010000000
VERSION     DARBFO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.044"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            149..796
                     /locus_tag="SCK51_001692"
     CDS             149..796
                     /locus_tag="SCK51_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEG1252326.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    925..1141
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1189..3834
                     /locus_tag="SCK51_001693"
     CDS             1189..3834
                     /locus_tag="SCK51_001693"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEG1252327.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4144..5457
                     /locus_tag="SCK51_001694"
     CDS             4144..5457
                     /locus_tag="SCK51_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEG1252328.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5447..6100
                     /locus_tag="SCK51_001695"
     CDS             5447..6100
                     /locus_tag="SCK51_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1252329.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6155..6706
                     /gene="radC"
                     /locus_tag="SCK51_001696"
                     /pseudo
     CDS             6155..6706
                     /gene="radC"
                     /locus_tag="SCK51_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6837..7922
                     /locus_tag="SCK51_001697"
     CDS             6837..7922
                     /locus_tag="SCK51_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1252330.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7919..9811
                     /locus_tag="SCK51_001698"
     CDS             7919..9811
                     /locus_tag="SCK51_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1252331.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            9818..10195
                     /locus_tag="SCK51_001699"
     CDS             9818..10195
                     /locus_tag="SCK51_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1252332.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            10349..11131
                     /locus_tag="SCK51_001700"
     CDS             10349..11131
                     /locus_tag="SCK51_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HEG1252333.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(11257..11988)
                     /gene="erm(A)"
                     /locus_tag="SCK51_001701"
     CDS             complement(11257..11988)
                     /gene="erm(A)"
                     /locus_tag="SCK51_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEG1252334.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(12046..12105)
                     /locus_tag="SCK51_001702"
     CDS             complement(12046..12105)
                     /locus_tag="SCK51_001702"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HEG1252335.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            12497..13159
                     /locus_tag="SCK51_001703"
     CDS             12497..13159
                     /locus_tag="SCK51_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1252336.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13394..13537
                     /locus_tag="SCK51_001704"
                     /pseudo
     CDS             13394..13537
                     /locus_tag="SCK51_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            13687..13887
                     /locus_tag="SCK51_001705"
     CDS             13687..13887
                     /locus_tag="SCK51_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1252337.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(14141..15820)
                     /locus_tag="SCK51_001706"
     CDS             complement(14141..15820)
                     /locus_tag="SCK51_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEG1252338.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(15822..16034)
                     /locus_tag="SCK51_001707"
     CDS             complement(15822..16034)
                     /locus_tag="SCK51_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEG1252339.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            16427..18157
                     /gene="efrA"
                     /locus_tag="SCK51_001708"
     CDS             16427..18157
                     /gene="efrA"
                     /locus_tag="SCK51_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEG1252340.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            18154..19914
                     /gene="efrB"
                     /locus_tag="SCK51_001709"
     CDS             18154..19914
                     /gene="efrB"
                     /locus_tag="SCK51_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEG1252341.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            20009..>20174
                     /locus_tag="SCK51_001710"
     CDS             20009..>20174
                     /locus_tag="SCK51_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252342.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILK"
BASE COUNT         6669 a         3249 c         4178 g         6078 t
ORIGIN      
        1 acgtttggtt gagcttatta ccgcagatgc aagcttttga acaccgtttt atttgtctca
       61 cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt acactaaata
      121 tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac tgccaaaagc
      181 aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg actcaggtat
      241 aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg caacgatccg
      301 cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg atgtcccttt
      361 tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa tcgcattagt
      421 aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa atgaaaatct
      481 aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa ttgatggaga
      541 aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg tgacagtagc
      601 aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg tcaaaggaaa
      661 tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt atgtcaatgt
      721 tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg agaattactc
      781 agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa ctcgtcgtaa
      841 ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac ttgttgacta
      901 aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa ctggcacatt
      961 cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt gaaggtagct
     1021 tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta actcacttga
     1081 gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat acgtcctaga
     1141 atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat gacggaagaa
     1201 aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta tcagcattgg
     1261 ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta ttcaatcgta
     1321 atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga tacgaccttg
     1381 caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg gctgcctggt
     1441 atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag agagcaaggg
     1501 atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga atggaaagaa
     1561 gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt agattacagt
     1621 agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt ttttgttacc
     1681 ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga tcctcaagca
     1741 cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc cttttatcat
     1801 atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac ccgtccagaa
     1861 acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta tcaagcgctc
     1921 atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat tgcagatgaa
     1981 tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca tgatccaaac
     2041 gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga tgatgatggt
     2101 acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc tcgtaaagca
     2161 atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat gatccacagt
     2221 gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac tcaatggttt
     2281 gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga tcaagcagtg
     2341 gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa tgtccatgat
     2401 tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta tcacaaagaa
     2461 acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg ggtacaagat
     2521 cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac aatgggctgg
     2581 ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt agtaaccggt
     2641 tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga atttactggc
     2701 gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca agggcggaag
     2761 atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa gtatggagca
     2821 gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt ccgcttcagc
     2881 tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc ttcacgtttc
     2941 gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg agaaaaatca
     3001 gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt aactgagcta
     3061 tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat ctgggatgat
     3121 ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga tgctgtttca
     3181 aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg tttattgcat
     3241 ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca gggaaattct
     3301 ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac agcttctaaa
     3361 gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc agaagtgaat
     3421 acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa aatcgatcaa
     3481 tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga gttgacgata
     3541 tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg tgcagaaatc
     3601 ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt agaaaaagaa
     3661 ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa tgaacgtttt
     3721 gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa agattattta
     3781 gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca ataagaatag
     3841 aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga acatcgttac
     3901 aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga cataaatttt
     3961 gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg tggatcatgt
     4021 atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt ttctctttgt
     4081 catactttta tttttcttat gtgttctatt tgttataata aaagagaaat gggggaattg
     4141 ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt tggttcacgt
     4201 cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga gagaaaagta
     4261 aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta tttgagaagc
     4321 ctattagaag aggctgggct gacagtaggt acatttactt caccttatat cgaaacgttc
     4381 aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat ccagcttgta
     4441 aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc tggtattact
     4501 gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa agtggacgta
     4561 gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc tgtaccaatg
     4621 ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga tacaattgaa
     4681 gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt aacaggaaat
     4741 attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa tgcaccgctt
     4801 tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat gtggggagaa
     4861 gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc gcttttaggg
     4921 caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta ctgtcaattg
     4981 gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc tcgatggcca
     5041 ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc ccataatacg
     5101 catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata tcaaatcaac
     5161 attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg attgcagcaa
     5221 gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat cgaattaagt
     5281 gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca attcggatta
     5341 gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg ttcactttat
     5401 ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga agtataatgc
     5461 agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt acgaagccac
     5521 acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt taaaattctt
     5581 aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg aatttggcaa
     5641 aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac gtttttcgca
     5701 aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag aagaacatca
     5761 gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt tattgaagaa
     5821 gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc aacgagcaaa
     5881 accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga agaaagaaac
     5941 attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg agatcccggt
     6001 agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa cagctggggt
     6061 tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct cttttcgtat
     6121 cttattttag ataagataga aaggagatgt ttttttggga aaacgattga tcaaagaagt
     6181 acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag ctttatcgga
     6241 tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg tgatgtcgat
     6301 tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg ctactttgca
     6361 tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca aagccttgat
     6421 cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg ttcgtaccag
     6481 ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag aacatttggt
     6541 ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt tcatcggttc
     6601 gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac gttattgtgc
     6661 agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttttaacca tagtataata
     6721 aataatagta gttaaagtag tttaagagga gatattaaag ggatatttta ctttaaataa
     6781 aaattaacaa ggtttataat ttgtagtagt taaagtattt aaagaggtgg gaacatgtga
     6841 aggttcaaag gatagaagtg gagaataagc cgtatccatt gtatttatta ctagataaag
     6901 aataccagct aatagaacca gtaatgaaat ttattaaata cttagataat actggtaagt
     6961 ctcctaatac cattaaagca tactgctatc atttaaagtt gctgtacgag ttcatggaac
     7021 agagaggtgt tattcttaat gatattaact ttgagttgtt agcagacttc gtaggttggt
     7081 tgagatatcc ttcagcatca aatgtaattg atcttcagtc aaaaaaagcc ataagagaag
     7141 aaacgacagt gaatacaatt ttaaatgtag ttatgagttt tcttgattat ttaagtagat
     7201 taggagaatt taaatcaatt gatgtattta aacaagcaaa gggaagaaat ttcaaaggat
     7261 ttttacatca tgttaataag ggtagatacc aaaagaatgt cttaaagtta agggttaaaa
     7321 agaaacagat aagaacattg agatcagagg aagttaagca aattattgat gcttgtcata
     7381 cgaaaagaga taaattaatt ttaatgctta tgtatgaggg tggtttaaga atcggtgaag
     7441 tgttatcgct taggcttgaa gatattgtca cttgggacaa tcaaatccat ttaacaccta
     7501 gagatgttaa tgttaatgaa gcttatatta aattaaggaa ggaaagaaca atacatgtga
     7561 gtaaagaact tatgtcactt tatacagatt acttgatata tgagtatagt gaggaattgg
     7621 agcatgatta tgtttttatt tccttaaaag aaggctattt tgggaaacca ctaaagtacc
     7681 aaagtgttct tgatctagtt agaagaatag ttaaaaggac tggaatagaa tttacatcac
     7741 atatgcttcg ccacactcac gcaacgcagc taattaggga aggatgggat gttgcgttcg
     7801 ttcaaaagag attaggtcac gcacatgttc agacaacgtt aaatacctat gttcatcttt
     7861 cagatcagga tatgaaaaat gagtttaata aatacctcga gagaaaggag cataagaaat
     7921 gaatgcttct agtaaaagga aaattattag tcagagtgag attagcaaaa aaatagctgt
     7981 aatgaatgaa gaaatgcagg ggttttgggc taataatagt tgggatataa gaaaatgtcc
     8041 acatccttct gccatagaat taagtaagaa tcctgcttta aggaatcgtt gggttcgttt
     8101 tgaacgtgtt aaaaatctgt ggttaagaac agaattgaaa tatttttatt tttaccattt
     8161 aaacaatgga atatggaatg caaaaactgt ctggattaga aaaggaacag taattaataa
     8221 aatgttagat ttcttagatt taaagtatcc tagcattact tcaattactg aagttcctat
     8281 tgaaaaagca atgacggagt atagaactta tttgacaaaa cggggtgtta gaattaccac
     8341 tactaattat aagattactg ctaatcaaga aaaaacacct gtaaaagcta attcctacta
     8401 tgttactaat ctaaaacaat ttatggagtt ttatgagaac ttttattttg atggagagga
     8461 gtgggataaa gacgtttggg atagacgtaa cttacctttg ccagatgata aggttaaccc
     8521 aacacaatat gaatatacaa ttaactttaa agggtttcgg aatacatatt ttaaacaact
     8581 tgtaaaaaga tattgtaagt tgagattgaa catggatagc ttttcctatg taagtgatat
     8641 tgcccaaaga cttaaagagt tctttaattt tctggacatg aaatttaaac aagttcagag
     8701 agtacaccaa ttaacgagag tggaaattga agcatattta agtgaactaa acatgatggg
     8761 aataaaacct agtacaataa ctgggaggat ctctatattg gaaggactat ttagtaccct
     8821 tcttaggcta gaatgggatg atgttccttc caaaatatta atttattctg aggactatcc
     8881 gaaaatacca agagcaaaac cacgctttat agatgaattc gtcctagagc aattgaacag
     8941 tcatcttgat aaattacccg aatatatagc tacgatgact atgattgttc aagaatgtgg
     9001 aatgaggata agtgaattgt gcaccttgaa aaaaggctgt ctattagagg acaaagatgg
     9061 agatttcttt ttaaagtatt atcaatggaa aatgaaaaag gagcatatag ttccaatatc
     9121 taaagaggta gctttactta ttaaagttcg ggaagataaa gtttcagagg aatttccaga
     9181 tagtgaatac ctctttccaa gaaaagatgg atcgccatta aaacaagaaa catttagagg
     9241 tgagttaaat aaattagctt atgagcaaaa tatagtggat aaatcaggtg agatttatag
     9301 attccatgcc catgcctttc gccatacagt aggaacaaga atgattaaca acgggatgcc
     9361 ccagcatatt gtgcagaaat ttttggggca tgaaagccca gaaatgacaa gcagatacgc
     9421 tcatatcttt gatgaaactc taaaaaatga atttactaaa tttcaggaaa aactggttac
     9481 caataatgga gatgtgcttg atctagatga agataatgaa gtcgatgatg tagagcttca
     9541 atggttcaag aaaaatataa atgcacaagt gcttccaaat ggttattgta gattgccagt
     9601 agtagcaggt ggttgtccac atgcgaatgc atgcttagat tgcactcact tctgtaccag
     9661 taagcaattc ttaccacagc acgaagaaca gttagagcgt acagaagagt tattagccat
     9721 agctaaggat aaacaatggc aaagacaagt agagactaat agccgtgtta aagagcgttt
     9781 agaacaaatc attggaagtt tgacggggta attatcaatg gataaacaag ttagaaatac
     9841 aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag acaagggaaa aagtagacaa
     9901 agcgatttct aaattttcga ttgaaggtaa agttattaat tttaattcaa tagcaaagga
     9961 agctaatgtt tctaaatcat ggctttataa ggaacacgat attaggcaaa gaatcgaatc
    10021 ccttcgtgag cgtcaaataa cagcaaatgt agtctcaaaa cccaagaaaa gttctcgttc
    10081 ggaggaaatc cttattaaaa ccttaaaaag aagagtaatg gaattagaaa aagaaaataa
    10141 aaaattacag aaccaaattc aaaaattata tggagatctg tataataaag aataattatt
    10201 aatctgtaga caaattgtga aaggatgtac ttaaacgcta acggtcagct ttattgaaca
    10261 gtcatttagg tatatgtcca atctagggta agtaaattga gtatcaatat aaactttata
    10321 tgaacataat cagttacgag gtgaaatcat gagcaatttg attaacggaa aaataccaaa
    10381 tcaagcgatt caaacattaa aaatcgtaaa agatttattt ggaagttcaa tagttggagt
    10441 atatctattt ggttcagcag taaatggtgg tttacgcatt aacagcgatg tagatgttct
    10501 agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa aaactaacag aaagactaat
    10561 gactatatca ggaaagattg gaaatacgga ttctgttaga ccacttgaag ttacggttat
    10621 aaataggagt gaagttgtcc cttggcaata tcctccaaaa agagaattta tatacggtga
    10681 gtggctcagg ggtgaatttg agaatggaca aattcaggaa ccaagctatg atcctgattt
    10741 ggctattgtt ttagcacaag caagaaagaa tagtatttct ctatttggtc ctgattcttc
    10801 aagtatactt gtctccgtac ctttgacaga tattcgaaga gcaattaagg attctttgcc
    10861 agaactaatt gaggggataa aaggtgatga gcgtaatgta attttaaccc tagctcgaat
    10921 gtggcaaaca gtgactactg gtgaaattac ctcgaaagat gtcgctgcag aatgggctat
    10981 acctctttta cctaaagagc atgtaacttt actggatata gctagaaaag gctatcgggg
    11041 agagtgtgat gataagtggg aaggactata ttcaaaggtg aaagcactcg ttaagtatat
    11101 gaaaaattct atagaaactt ctctcaatta ggctaatttt attgcaataa caggtgctta
    11161 cttttaaaac tactgattta ttgataaata ttgaacaatt tttgggaaga ataaagcgtc
    11221 ctcttgtgaa attagagaac gctttattac tttaatttag tgaaacaatt tgtaactatt
    11281 gaaaatagaa agaaattgtt ccttcgatag tttattaata ttagtgacat ttgcatgctt
    11341 caaagcctgt cggaattggt ttttagtgaa aagaacacga tattcacggt ttacccactt
    11401 ataaacaaaa gatcgatact ttttgtagtc cttctttgaa atcaatggtt gatgtcgttc
    11461 aagaacaatc aatacagagt ctacacttgg cttaggatga aaatatagtg gtggtacttt
    11521 tttgagcatt tttatatcca tctccaccat taatagtaaa cccaaagctc gttgcagatt
    11581 ttgcaatctt ttcgcaaatc ccttctcaac gataagatag ctatatttag cctgactttc
    11641 aaaggtaatt cttttgacaa tatccgtact gatgttataa ggaatattac catatatctt
    11701 atagtttata tgttttggga aggaaaattt tagaatatcc gtttgaatca cttttatatt
    11761 ctcagagggg tttaccgctt ctttagtcac ttgacataag cctccatcaa tttctatagc
    11821 agtaactgat cgactcattt tgactagctc tttggtaaaa tgtccttttc ctgatccgat
    11881 ttctattacg ttgtcttgtt tactgatatt cgtgtgattc aatatttctt ttacatgctt
    11941 tttagaagta ataaaatttt gcgtgtcttt agggtttttc tggttcatta taaccttctc
    12001 cttactggtt ataatgaact gacattaagc gttcattata accaattaat tttgatttgg
    12061 ttgataacga actttattaa taacaaatat agaaaaagta cccatacttt tatctccttt
    12121 ccattttttt cattatgaat gagataaagt aatttctact actgcgatac tggtgcacat
    12181 aattgaaacc tccttatgta actgaatatg attgtatcat cttttcattt tagaataaac
    12241 ctttatatgt aattttgcac ttgacacttc agatggacgt tttcttcaaa ataatatgtt
    12301 ttgaaacttg ataagagaag ccactgtctt tactaaaaag taaaggctgc gataaaggac
    12361 agtgacttta ataaagttgc actgttgtat tgtctttgga taaaattgtc taaagggaag
    12421 ttttatcccc aacgaaaaaa catctaaagt ggtaagtaat gaaagaatgt cgataaagta
    12481 ggaatggggg atctttttga atacaataga atcacagttt gataaggttg cagaagatta
    12541 cgatttcgtg aatgagcttt tgaatgatta ttcattcttt gtgtctaata tgtctccaaa
    12601 gaaaggcaga gcattagata tcggatgtgg ctcgggtttg ttagtggaga aattagcaag
    12661 ttattatgat gaagtggtag ggattgatat ttctaatcaa atgctcgatc ttgccaaatc
    12721 taaacgtcaa ctaacaaata cggtctatct gaatatgaat gcagaacaac ttaattttaa
    12781 tgagaagttt gattttattg taagccgaac aacctttcat catttggatg atatagccag
    12841 cgtaatacag caaatgaagg aactgttgaa tgaggaagga agaatagtta ttcttgataa
    12901 tgtatctgaa gtcgagacac cgcctaccta tgtttataaa ttaggggcaa tccaagagtt
    12961 tttaccgcat tgtttcaaat ttggaataaa aaatgcgatt agaatttata atcacaacac
    13021 atctaaatca tggcttgagc atttagcttc agataagtac ctatctgaac aaaattatta
    13081 cgatttatat gaaaagttgt tgcctggatg ccaattccat aaaatgggtt gggcaatggg
    13141 ggttgtctgg acaaaataac ttaaagctgt ggctttgaaa agttgaattt gtgattgttt
    13201 tcagagctat cgggtgcttt agtataaata acaatgaata aaaaatcagc ctgtatctta
    13261 tgttactatt aagtaattta agatacaggt tttactattt ttgaaataag taccttccac
    13321 ttcaaataca tgtattcaaa gtgaaagtat tgatttaaat gattcttttg taatttactc
    13381 ttgataatat gaaattccat cgcagcagga tatgaatttt accaaaagga tacaaaagtg
    13441 tggagaaatg atggggatca ctgtactgga ccacttgatc atcggacgca aacgatattt
    13501 tagtttgaga gaagaaggaa tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg
    13561 aagcaagtgt acaattatat tgtaattttg tttgggaata gaagttgaaa catctgttgc
    13621 acctctatcg tacacgagca atgttacaat ttaaattagt gcttttggca cgaggaggat
    13681 attattatgg aaaacggaac agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc
    13741 tcacgtgaag atggaagcga cgtattcgtt cacttctcag ctatccaagg tgacggattc
    13801 aaaacattag aagaaggtca agcagtgact ttcgacgttg aagaatcaga ccgcggccca
    13861 caagctgcaa acgttgtaaa agcttaattt tatctgtaaa tcaaattgat ggtagacatc
    13921 aatttttacg agagaacaaa gcgagtaaca aaaatacctg ccacaagaga caatttctct
    13981 tgtggcaggt atttttttgt atgaaaaagt aaataaattt aagaggctat gaaagagcca
    14041 ccttgtatca aggaataaga acaaacaaaa atggcttctc atgtttttca tttgttttct
    14101 cttatattca ggacaaggtg ctgatttcat aacctcttat ttattcttca tctggtgtca
    14161 ttaccattgg caaaatcatt ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg
    14221 cgccagtcat cgcttctttc aatttatatt ctgaacagtc aggtccattc aaacattctc
    14281 ttaaagcgtg gaataagatt cgctgagctt cattgatcat ttctcctgat tctcgcatgt
    14341 agacaaatcc acgagagaga atatctggac cagccaagat ttcttttttc ttgatatcca
    14401 ctgtcgcgac agctaataca agaccttctt ctgaaagcag gtgacggtca cgtaaaacga
    14461 cattaccaat atcaccgatc ccgctaccat cgatatacac atctccagcc acaaaatgcc
    14521 cagcaggacg cgcagaatcg ctagttaacg ctaaaacatc gccattttcc atgatgaagc
    14581 agttttctgc aggaacacct acatcttgtg ctagagaagc atgtactttc aacatgcgga
    14641 attctccgtg gacaggaata aaatattttg gcttcatcaa acgaagcatt aatttttgtt
    14701 cttcttgtcc accgtgaccg gaagtatgga tgttattgat ttttccgtga atcacttctg
    14761 caccagcttc agacagcaaa ttgatcaaat gattgacact tgttgtatta ccaggaatag
    14821 gtgaacttga aaagatcact gtatcgtctg gctggatttg gatttgacgg tgagtaccat
    14881 tcgcgatgcg gctcaacgcg gccatcgctt ctccttgaga acctgtacat aggatcatcg
    14941 tttcattggc aggcaagcga ttgatttcgt gtgcatctac aaatgtgccg tctggaactt
    15001 tgatatatcc taaacgttga ccgttgacga tggcattttc catgcttcgt ccgaaaacag
    15061 cgattttacg accagttgcc acagcggcat ctgctgcttg ctgcaaacgg aagatgttag
    15121 aagcaaagct ggcaaaaatg atacgtcctt cgatttttga aaagatcttc aaaatagaat
    15181 gcccaatcgt tttttctgat ttagtaaatg ttggaatctc agcatttgta ctatcagata
    15241 aaaggcacaa gactccttct tccccgatac gagccatttt atgcaagttt gctggttcgc
    15301 caacaggagt gaaatcgaat ttgaagtctc cagtcgctac gatatttcca gaaggtgttt
    15361 tcaccacgat ccctaatgca tctgggatac tatgggtcgt tctgaaaaag ctgacagaag
    15421 tctttctaaa acggatgatc gtatcttcat tgatctcatg gagctctgca tctcgaagca
    15481 agccatgctc atctaatttg tttgtgatca acgctaatgc gagaggacca gcatagatag
    15541 gaatatttgc ttgtttcagc aagtagggta ttccgccgat gtggtcttcg tgcccgtgag
    15601 taatcaccaa ggcttttact ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga
    15661 catagtcaat tccaagtaaa tcgtcttccg ggaatttgat cccggcatca atcaaaatga
    15721 tttcatcttg gaattgtacc ccatacgtat ttttcccgat ttcacctaat ccaccaatag
    15781 caaagacgcc tgtctcgtta tttttgatgg aaagtttcat attaaaactc cactaatgag
    15841 aaatcagcat tttctttttc gtactctaga tgacttcctt ctagaagttg aacgaattca
    15901 atattgtatg gggtattttc ttccacacgt tggcgagcgt ctacatcgct ttttgcctca
    15961 atgtaaagag attttgtttc ttcccgttta gggttgcgta cttttgtttc ttgataataa
    16021 actttataga tcattgtttt gtctccttta tctgcgtaaa gcagtgattt agctagttga
    16081 tttttttaga caacacaaaa cgaggggaca agccacctgg tttttcatat gctttgacta
    16141 gaatactgga ttcgagtcaa agagacaagc ctcgtttttg cttatctgtt cggatatttt
    16201 ttcaaccgta ccttctgtat aaaaaatata caacaattaa ttgttattct agcataaaac
    16261 agcaaataag tatagaaatc aactagattg aaagaaaaaa tcaatgttta tcatcccgct
    16321 ataatcataa tgactttttt atgagagaag tgttctatta ataggctcgt tgtgctatca
    16381 tttttagtaa tgaaaatgta acaaaagatt aaaaggagat gagaaagtga aactaatgtg
    16441 gcattatacc atgcgttata agaaattctt gtttttcaat ttcatatgcg tatttggttt
    16501 tattttgatt gaattaggac ttcctacgat tttagcacgt atgatcgatg taggaatccg
    16561 caatgatgat tttgattatg tgaagcaaca agggatattt atgatcgtta ttactgtaat
    16621 cggtattatt ttgaatatct tgttaggtta ttttggttcg aggattacga ctaatatcgt
    16681 agcagatatt cgagatgatt tgttcgaaaa gatccaaagt tattcgcatc aggaatacga
    16741 aaatctaggc gtatcttcat tgatcactcg tacaacaaac gatgcttatc agatcatgtt
    16801 gttcctgcaa aatattttac gtatcggttt tatggcacca atgatgttca ttgtcagttt
    16861 gtacatggtc atgcgtacaa gtgtgacact cagtttgtat gtagtaggtg cattaccttt
    16921 actattattg gcagtcgtag cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca
    16981 aaaaaactta gataaaatca acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat
    17041 tcgtgcattc gtcaatgaaa agtttgaaga aaaacgtttt gctgtagtca atgagaatta
    17101 tgcttctagt tcaaaaagct tattccgttt gatggctgtt gcacaaccgg gtttttattt
    17161 cttatttaat atcgtaatgg tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg
    17221 cgagcttcgt gtaggagatt tgattgcgtt tatcgagtat atattccatg ccttgttctc
    17281 attcatgtta tttgctagtg tgttcatgat gtatccaaga gcagctgttt cagctggacg
    17341 tatccaagaa gccctagatg ccaaaccttc gattgtagaa gatccaaatg gtatatctga
    17401 tacaaaaact aaagggtata tcgaatttca aaatgtaaca tttgcttatc caggccattc
    17461 gcaagagcca gtgatacgaa atgtcagttt tacagcctct ccaggagaga ccgttgcttt
    17521 tattggaagt accggtagcg gaaaatctac gttgatccaa ttgatccctc gcttttatga
    17581 tgtgagtgaa ggaaagatct tgatcgatgg agtagacgtg agagattatc ggctaagcaa
    17641 gttgcgagat aagattgggt ttattccgca aaaagcgctg ctatttactg gaacgattgc
    17701 agataatttg cgatacggga aagaagatgc tacgcaagaa gaaatggaac gtgcagctga
    17761 gattgctcaa gcttcagaat tcatttctcg aaaaccagat ggatttaatg aacatttatc
    17821 agaaggtggt tccaattttt caggtggaca aaaacaacga ttggcaatag ccagagccat
    17881 catacgccgt ccggaaatct atatttttga tgacagcttt tctgcattag attatcaaac
    17941 ggatgcgaag ttacgtgctc gtctgaaaaa agaaacgaca gaatctactg tattgatcgt
    18001 agcacaacgt gtgggaacga tcatgcatgc agataagatc atcgtattaa atgaaggaga
    18061 agtcgtcggt atggggacgc atcgggagtt gttggaaaaa tgcccgattt attatgatat
    18121 tgccgcttct caattatcaa aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa
    18181 cgtttagctc gatatatcag accgtatcgt gagacttttc ttttagtgat cctatttaca
    18241 gttctgacag tggctttcaa cgtggctttg ccatatgtta caggacttcc tactacagaa
    18301 atcagccgta atattgccaa tggcgaatca atcaattttt cttatatatt caaatgtctc
    18361 atttggatca cagttgttgg aatagggtat tgtatttcgc aattactttc tggtgttttg
    18421 atgacaaatg tcgttcaaag tgccatgcat gatttgagaa gagacataga agaaaaaatc
    18481 aatcgtcttc cggtttcata tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg
    18541 acgaatgatg tagatgcagt cagcggtgct ttacaacaag cttttattgg tatcgtaaat
    18601 gcgatattgg gaattacgtt ggctgcagca atgatgtttt atatccagcc gatgatggcg
    18661 ctgatctcta tgatcatgat tccaacatcg atctggatct caaaacgagt gatcaatgct
    18721 tcgcagaaat attttcaaag catgcagaat tctcttggtg agctaaacgg ctacgtccaa
    18781 gaaaatatga caggttttag tgtattgaaa gtatatggac gcgaaaaaga aacattggaa
    18841 ggattcaaaa aagttaatca cagcttgaaa tactttgggt ttcgtgcagc attcatttct
    18901 ggcttgatga tgcctctagt tcaattaact gcctatgcta cttatattgg tatggctgtt
    18961 ctaggaagct tttatgttat tactggggcg atcgttgtcg gtcaactaca agcattcatc
    19021 caatatattt ggcaaatcag tcagccaatg gggaacgtga cacaattgtc ttcagttttg
    19081 caaagtgcat cagcggcaac gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa
    19141 gaaaacaaag tagatgcttc actgccagaa acgattttag gagatgtgac attcgatcat
    19201 gtcgattttg catatgatcc gaagaaacca ttaatccaag atttgaattt tgaagtgaaa
    19261 gctggtcaaa ccgtagctgt ggtcggtccg accggtgccg gcaagacgac attgatcaat
    19321 ctattgatgc gtttttatga cgtagacaaa ggagcaataa ggattgatgg aatcgacacc
    19381 aaatcaatga gtcgcagtga tgtgcgttcg ctatttggta tggtcttgca agatgcatgg
    19441 ctttatcaag gaacgattgc agataatatc cgcttcggaa agcttgatgc gacagattat
    19501 gaagtagttg atgctgcgaa aacagcaaac gtagatcatt ttatccgaac gatgccagat
    19561 ggatatgata tggccatcaa ttctgaggga gacaatgtgt ctttaggaca aaaacagttg
    19621 ctgacgattg cccgagcagt ggtctccgat cctaaaatct tgattttaga tgaagcaaca
    19681 agttcagtag acacccgttt agaagcgttg attcaaaaag caatggatcg cgtgatggag
    19741 ggacggacaa gtttcgtcat cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt
    19801 ctagtgatgg atcaaggtca gatcattgaa aaaggaacgc atgagagttt attggcacaa
    19861 ggtggatttt atgaaaaact gtataacagc caatttgctg aggaagcaga ataagtcata
    19921 taagaataaa acagtttaaa acttattttt tgttctctat tcttgataat ggtaaagtaa
    19981 agttatcaag aaaagtgagg acacggagat ggaacagttt ttaaatgaaa aaattactga
    20041 agccaaaaga gaagttgatc gtttaacgca cgtctgtcag acgaagcctt ttgattcagc
    20101 tgattttttt gaaaatgagt ttcaaatcga acgcttggca gcacaaatag aagtgtacga
    20161 attaatttta aaaa
//
LOCUS       DARBFO010000044        19476 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000044 DARBFO010000000
VERSION     DARBFO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.045"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(22..996)
                     /locus_tag="SCK51_001711"
     CDS             complement(22..996)
                     /locus_tag="SCK51_001711"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEG1252343.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1076..1951)
                     /locus_tag="SCK51_001712"
     CDS             complement(1076..1951)
                     /locus_tag="SCK51_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEG1252344.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2081..3988
                     /locus_tag="SCK51_001713"
     CDS             2081..3988
                     /locus_tag="SCK51_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEG1252345.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4529..7807)
                     /locus_tag="SCK51_001714"
     CDS             complement(4529..7807)
                     /locus_tag="SCK51_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEG1252346.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8078..8623)
                     /locus_tag="SCK51_001715"
     CDS             complement(8078..8623)
                     /locus_tag="SCK51_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1252347.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8635..9327)
                     /locus_tag="SCK51_001716"
     CDS             complement(8635..9327)
                     /locus_tag="SCK51_001716"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEG1252348.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9346..9630)
                     /locus_tag="SCK51_001717"
     CDS             complement(9346..9630)
                     /locus_tag="SCK51_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252349.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9643..10206)
                     /locus_tag="SCK51_001718"
     CDS             complement(9643..10206)
                     /locus_tag="SCK51_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG1252350.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10434..11096
                     /locus_tag="SCK51_001719"
     CDS             10434..11096
                     /locus_tag="SCK51_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEG1252351.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11220..12416
                     /locus_tag="SCK51_001720"
     CDS             11220..12416
                     /locus_tag="SCK51_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEG1252352.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12517..13893)
                     /locus_tag="SCK51_001721"
     CDS             complement(12517..13893)
                     /locus_tag="SCK51_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1252353.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13930..14796)
                     /locus_tag="SCK51_001722"
     CDS             complement(13930..14796)
                     /locus_tag="SCK51_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HEG1252354.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(14885..15910)
                     /locus_tag="SCK51_001723"
     CDS             complement(14885..15910)
                     /locus_tag="SCK51_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1252355.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15928..16602)
                     /locus_tag="SCK51_001724"
     CDS             complement(15928..16602)
                     /locus_tag="SCK51_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1252356.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16786..17679)
                     /locus_tag="SCK51_001725"
     CDS             complement(16786..17679)
                     /locus_tag="SCK51_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1252357.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17753..19450)
                     /locus_tag="SCK51_001726"
     CDS             complement(17753..19450)
                     /locus_tag="SCK51_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1252358.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLAKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5683 a         4189 c         3446 g         6158 t
ORIGIN      
        1 ttctttccct cttttttttg tttattcgaa ttctttttct tccagacgga ataacggact
       61 catcggtgta ttttcatgga tgcgtttgac cgcttcaccc attaattctg aacatgtaac
      121 gattgttaag ttttctggat gacgattttc ttctgtgaaa acagaatcag tgatacaaat
      181 atctttgatt ggtgcattgt ctaaaatact tttcgcatga tcggacaata atccatgtga
      241 cgcacaggca tatacttctt ttgcgccgtt ttcttttaat acatttgctg ctcgtgcaag
      301 cgttgctcct gtgttcaaga tatcatctac catgatacat gtctttcctt gaacatcacc
      361 gatcacataa ccaccctcag ttgaagcatc atctgcatga tctacgatgg ctaatgtgct
      421 gttcaaatat tctgaaagac tacgtgcacg ctgaacaccg ctgttttttg gcgaaacaac
      481 tacgatgtca tctcccgtca gacctaattg acggtaataa tgagcaaaca acggcatcgt
      541 aaataaatta tctacaggaa tatcaaagaa tccttgtact tgaaccgtgt gcagatctaa
      601 agtcagtact cgagttgccc ctgcttcttc gatcaagtta gcgatcaatt tcgcagtgat
      661 cggttcatga ggtttcgccg tgcggtcttg cctagcatac ccatagtaag gcaatacgac
      721 attgatcgtt ttcgcacttg ctcgcttcat tgcatcgatc atgatcagta gttccatata
      781 gtagtcattg acaggtgcat tcgttgattg aacgatatac acatgatccc ctcgaatact
      841 ttcttcgata ttgatggaaa tctcgccatc actaaattgc ttcacgacgc ttttacctaa
      901 ttctgttccg aacactttcg cgattttttc agctaatgga cggttgccat tcaaactaaa
      961 aatcttcaac gtgtcatcct gatacttatt actcatgtag tcctccccaa aacttctcta
     1021 ttcttcgata gtttaacata tttttccaag attttctgtt aatttcagca aattcttaag
     1081 gaagtgaaac tgttccgtta ctccaactgg caggaatgac tttatgataa tcgccccagt
     1141 tgtcgtgtac tttgtaataa cgttctcctg tttcagtttc aaaataagcg tacgctgtca
     1201 cccaatgatt gccatatgta ctgccaagta ctttcaaaat accaatcatc acaggtttgc
     1261 cttcacgtat acgttttgta gctcgctgcc atgaacctag aagagttgct cgtgcacgct
     1321 catgattgcc attcttcttt aaaaaactag aaatccctgt actaacctga aaaggaatcg
     1381 taggaagtcc tagtggttgg atctggctac gcaatgcctt cgtcaaaacc aacgaatcag
     1441 ccgtattttt cttgcgaatc tcatttggaa gcatccaccc cttacgatag tcttgataat
     1501 aggcaaggag aactgctgcc gcgtaagtac cacaaagata gcctgagggg gtagcccatt
     1561 tcttatacct caaaaaacgt tcttctggca atcccaccca gcaatttgcg agttcctttc
     1621 ctgacctttt ttcttcgatc aaaacgggaa tatcttcttt tctgttgatc catctagata
     1681 actcgattgg ctcttcattg atcagcttcc attttttaca agaaagaccc tcttgataag
     1741 tatattttgc tggagaatat tctaccggat gtcctgattg atcgataatc acataacttt
     1801 gatcttcaaa aaccagagct aaaaaaagca atttactttt tcttgactgc agctgctgag
     1861 tgctggctaa ttttttaatt tttttatcta ctttgaaata taaaggttta tccactactt
     1921 gattcagttg ggatcgcctc ctttttttca ttataagtga aggattgaaa aaaacaaatc
     1981 agtttgattg ttcgaatttt caatttttta tgttctattt gttaattcct tcattttgta
     2041 agataatggg cacacgaaac aaaaaaggag cgatcgatga atgaacaaag aacgattgaa
     2101 acaagatttc tatgaagcag tcaatgaaga atggcaaaaa acagcaaaaa tccctgcaga
     2161 taaaccagct acaggcggtt tccaagattt agttgacggg atcgaccaac ttttgatggg
     2221 agaaacagac gatctgcttg cccatccaga aaaagtgtct tcagaaaaaa tgaaacattt
     2281 ccttgcatat tatcaactgg caaacgatta tgaccgaaga aatgagttgc atgctcaacc
     2341 gcttcaacct ataatcgaaa aagttgaatc attgcagtct ttcgacgaat tgaataagca
     2401 atttccagaa tggctgttgg atacattacc attgccattt aatctggatg tagatgcaga
     2461 tatgaagaat gcgcaaacga acgccttctt tgcttatccg ccttctttat tccttccaga
     2521 taagacgtat tatgcatcag accatcccaa cggtgcacaa ctaataggcg ttttctttga
     2581 tatggctgtt cagttattag agcttgccgg caaagaaaaa agtcaggcag aagcaatcac
     2641 ggaacaagca gtgcaatttg ataaattgat tgctcctcat gtaaaaagtg cagaagaaaa
     2701 cgcagattac agcaaaatgt ataatccgca atcttttgat acatttatta aatacacaga
     2761 taaactggac ttgaaaaaac ttgtcacggg tttgattggg acaactccag ataaagtgat
     2821 cgtaactgat ccagtgtact tcgatcattt gaatgacatc ctgacagaag ctcattttcc
     2881 gttattcaaa agctggatga tcattaaaac agtacgttct cttagtggtt atctatcaga
     2941 agaattccgc caagtcagcg gaatctattc tcgcgtattg tctggaacag acgaagcaat
     3001 gcctcagaaa aaagcagcgt attaccttgc ctctggacaa ttcgatcaag tagttggcga
     3061 ttactacgga aaaaaatatt ttggcgaagc tgctaaaaaa gacgtagaac aaatggtgaa
     3121 aaaaatgatc gccgtttata aaaaacgtct agaagaaaat gactggcttg gggatgcaac
     3181 aaaagaaaaa gccgtcataa aattaaataa cttaggtatc caagtaggct accccgataa
     3241 gatcccagca ttattcgacc agttcaaaac agtacctgca gatcagggtg gtactttgct
     3301 ttcaaatact cttgttttca gcaaactcac attaaaagat cgtttcagca aatggaacaa
     3361 accagtggat cgttcagaat gggaaatgag tgctgacacg gtcaatgctt attatcatcc
     3421 atttcggaat gtgatcgtct ttccggcagc gatccttcaa gcaccatttt acagtctaga
     3481 acaatcaagc tctgctaatt atggtggaat cggagctgtt atcgctcatg agatttccca
     3541 tgcatttgat aataacggcg ctttatttga cgaatatggc aacttgaaca actggtggac
     3601 agaagaagat cttacacaat ttcaaaacaa agcacaagca atgatcgaag aattcgatgg
     3661 tctgcctttt gctgacggaa aagtaaacgg aaaattaacc gtttcggaaa atattgctga
     3721 tgcaggcggt ctaagctgtg cattggaagc agcaaaaacg gaatcagatc tttctatcga
     3781 ggaattcttt gtcaattggg caacgatttg gcgtacaaaa gcaaaaaaag aataccaaca
     3841 acttttactg caaatcgatg tccatgcccc tgctaaactc cgagcaaacg tgcaaccaaa
     3901 aaaccttgac gaattctatg aaacatttgg tatcacggaa caagacgcta tgtatctagc
     3961 gccagaaaaa agagtgcgaa tctggtaaga ggttgtgcca gaagtggtca gcttcaagaa
     4021 ataagaagga atttgggaaa atcgcttctc aaatttttgt gacatttcgg cttatttttc
     4081 gaagaagtta cttctggaat accatgtatt agaggttgtg aaaaagctgc ttagtttcaa
     4141 gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt tgacttacta
     4201 ccgagaaact ggcttttgaa caccgtttat cgagaggttg tgaaatcagc gtcctgacct
     4261 gagtattaga gaaaaaaacg gaaaaatagt tcctcatatt ttgccgtttg ttgagctaat
     4321 accgagggtc agctgattga acaccgttta gtggagagat tgtgaaaaag ctgtcctgca
     4381 tcaagtaata agaacagcca atcaaaagca gcaaggagaa cctcatctat aaaaagatag
     4441 aaggaagtga gacatctatt ggtctcattt ccttctattt ttactttcct cagtcaattg
     4501 ttttacatca taaaaggaga aattccactt atttttttcg ctgtttcatg acataaacaa
     4561 ctacaccgat taacaaaatt cctccagcta atgctagcaa cgggttatta aattctcccg
     4621 tttttgggag tccttgagtg ccattttttt cactttcttt tgctgtccta tccggatgag
     4681 aaggttttcc atttggtttt ccatttggtg attcatttcc tggattttct ctttctggtt
     4741 tgtcatttcc cgggggcaca tcggcagttt ctcgcacagt gatcgctttt tgctgggtaa
     4801 cagattctgt atgagcagac catttttccc catcaaatat ttccgaacga taagtgactt
     4861 ctagcgtata ttctcctttt gaaggtgctg aaaaaatagc ggtatgatta gaaaattgag
     4921 cacttgactg tgtttcaacg atttggatct tttctgggaa ataacgcata tcttcaaaga
     4981 caggcgttcc ttgatttggt gtgaaagttg acacaaaaac tgtaaaaggc ttcagttcaa
     5041 tgatatttcc ttctgcattc tccgccacac catttgcttc ttgtataata atcggattat
     5101 tttgttcatt tggttttgtc tgatttggtt cttgtacgat tgcatactct ggatattccg
     5161 aagacggtga ggtttctccg tcaaaaatag cacctacaga taatggaaga ccatccactc
     5221 tttccttttc tgtttgacga ttccccggtt taatctgcat cagtccatga acatggatac
     5281 ttccattttc atagcgcgta acaggctgct gaccttcttt cgaacatgta tttagagact
     5341 cgatccaatc ttttgatatg gtatgtccaa gagtattttc tgattgtttc tctcgttcat
     5401 tccaatagta gttcaacgag tggtagattt gatcttcgtt ttgattcaat gggcggatct
     5461 gctctctcat ctcaagattc tctgttcgga aagataagat accagaagca tggtagtctg
     5521 tgtttcctgc tgaagaggta taaagagcca cattatatga accgttatta aaacttgtac
     5581 tgctattgac gataatatct gttccgctat tggaatcgat tcctttcgca agattttcga
     5641 aagacaaact attgatcaat tcatgagggc ccgttaaaga agaaccgccc attttgaatc
     5701 cattaccgtt accttctccc tcaattcctg gtacccagcc gtttcgataa gcaactgagt
     5761 ttttgatgat tacttttccg atcgaacctg tttcattttt agcaaaaagg tcccaaccgt
     5821 catctgcatt atgatacgcg atacaaccgt caaatacatt tccttctcct attgtcagtt
     5881 tggccgcaaa tccatcggca tcttcaaatc ctggatcggc attttcaaaa gatgtacagt
     5941 tcttgactaa gttatgcgcg ggccatgctt cgaaaggatc atttgagcta ccgctgattt
     6001 ggattcctgt atttccattc cgataagcat gaatctcttc gatcaaatta tgatttccag
     6061 aaatctgtaa tcctttttgc atagctgcac tatttgtaat gtcaaaccct cgtaaacccc
     6121 agtagtgact gaacagtgtc atcccatttc cagttccttt aaaatcaagt acaggacgct
     6181 tgttttctgg atttttctct gcaatcaact gaatcgggtt ttctcttgtc ccgttaacgc
     6241 cttttggaat cgtcaatcct ctttcgaagg aataagttcc aggcgccatg acgattgtct
     6301 gcccagcata tgcgtatctc aatgcttgtg tcagagataa tggattttgt ctgcttccgt
     6361 ttcctgcatc tgacccttct ggtgttacat aaactgtcga atatttaggt ttacggtagt
     6421 ccactgtatg aagaatctct tttgtttcat aactagtcat ctctacgtat tcttctggcg
     6481 gatagttttt atctggcgta aaactgaaag taaattcgtt cgttccttcc atcagttttg
     6541 tctgataatc aaattcccga ttcgcagtga tagccaagtt ttttgcgttc ttaagcacct
     6601 ctcctgttgc tttattttta attgtcaata atccgtcagc atttgctcta aaggacatag
     6661 tatgatcttg gtttcctgaa tgattactag aggtcacagt gtaaatcgga tcaatcaagc
     6721 gagtaggtct ttcttgccca ggaggatctg tcgcaggatc tgagtaagca acttcgattc
     6781 cttctacctt gatcgtcata ttccttgctg cagcaaatcc tgcataaata accgattcat
     6841 ctgaagcgta taatttttcc cagtcataca tgatgtcaga agccatttct tctcctgttt
     6901 caggatcgta ataatatgac tctaaaccag tattcgtttt tttcatctct acgtccagtt
     6961 tagtaatcga gttcgtggcc ggaaacaaat tcccatgacc gtttccgaat atattataag
     7021 aacctgtttc tttttccaag aaccctgctg aagtctctaa aggtgttgtt tccactgaga
     7081 cactcccagg agctggcgca gcttgcggat cttcagatga tattcctgtg attgcccgtg
     7141 tccccaatcc aagtcgcatc gtatatttgc tgccgctcgt gttgaccact tcttgtctat
     7201 ccgagtccca gacatattcg atccgactgc ctagtatggc aaatgaattt gaataaaatg
     7261 cctgcccaaa caaatgattt tttggtactg cgtctcttac cattaaagca aatccttctt
     7321 gggcattcgt ataggtccaa gattctattt cgatcgtcgc ccgcatccta aagttgatgt
     7381 ctttggggat tgcttgataa taataggtca ttccatctga tccgcttgac tggaattttc
     7441 cgccgttatt cgtggaagag agccgcacgc cgtcttctaa tggttcaatg gcatacctac
     7501 tagcacttgt gctttctccg aaaacaaccc ctttccactc ttcatttgct tcttcagatt
     7561 gcttttttgt cttttcattt tctgattctt tcatcatagg atcatttcct tcttccgtat
     7621 ccatatcttc actagttctt ggtacttcct ccattgttga catcccttca atgttcacgt
     7681 gtgcattttc ttcgttcaca gactgttgtt cgttagcagc aacttcaacg acagaaatta
     7741 aaaccggggt actgagcata aggagattca tcattagcgc actgtatttt atacgttttt
     7801 ttaacaagtg tattcctccc gtaacaaatt agaaagcgtt tacttaaagt attacctaat
     7861 ttatttttac tgtcaataaa aaacaaaatt ataataagtg tattttataa aaagaaaaaa
     7921 tataacttaa gttgttgata ataaagagat ttattaacgt aaaaagaata agttttttta
     7981 aaataacgta taaaaaagag cataaaacaa agtcgtttgt tttatgctct tcttttatta
     8041 ttttgtgctt tttattttat caaatcactt tgttttttta taaaatttct gcgcctagtg
     8101 tggactcaaa atgtctcaat gcccattcat gtccaacggg atcgaaactt gccacggcat
     8161 ctttgaaaat atggagtttg tatcctagat tatacgcatc gactgctgta tgcaacacac
     8221 agatatctgt acatacgccg gtcaaataaa tatctgtgat ctggcgttct cgcaaacgga
     8281 tgtctagatc tgttccacta aaagcggaat aatgccgttt gtctatccaa taaacatttt
     8341 cctcttgctc atagtcttgg tataaacttt ttaaagagcc gtacaattcc cgtccacccg
     8401 ttcctatcac attatgcgga ggaaataagc ggttctccgg atgaaaggaa tcttttggat
     8461 catgcgcatc aatggcaaaa acgacaaaat ccttttgatc aataaacttc tttgtatgag
     8521 cagtcagctt tttttcaatt acttgtcctg ctgtcccagt agtcagcttt ccatcctctg
     8581 caacaaaatc atatgtgtag tcaatcgaga tcaatgcgtt catcttagtc ctccttatac
     8641 gagatgtttg acaaattcga tcaccatttc tgctgatcgt ttgcctgcgt cttcgataaa
     8701 ttcgtcaaag ctttgtgtcg ccgagtgatc tgctgtgtcg ctcattgcac gtacgatcaa
     8761 aaatgggata ttgaactgac gagctgtttg tccaacagcg gctccttcca tttcgcaagc
     8821 taatgcttca gggaaattcg ttaagatttc tttgatttta tctggcgagt cgacaaatgt
     8881 atcacctgta acgatcaatc cttcttttgc gttcaaattc gttgcttttg ctgctttaac
     8941 catttcactg cgcaaatatg tgcttgcttc ataatataga ggcattcctg gtaattgtcc
     9001 tggtttgtag ccaaatcctg ttgcatctgc atcaaaataa gctactttgt ctgatataac
     9061 gatgtcacct acttgtaagc cttcaccgat tccgccagct gaacctgtat tgatcaccat
     9121 gttcacgccg tattgttgga tcaacagact tgtggtaata gatgctaaga cttttcctat
     9181 accggaacgt acgacgatca cttcgtggtt acctaatgaa cctgaaataa ataaagcgcc
     9241 tgcgcgttcc catgataatg gttcgcttag gttctctctc aaaattttta cttcttcttc
     9301 cattgcgccg ataataccga ttttcatctt tttaattcct tctttctata taaatgcgat
     9361 tgctaaaaaa accacacata acattaggat aacaataata agagatttca tcaaaaaaga
     9421 gttccatttg gtcgtctttt cccgttcgct gatccttgtt ttcgtgattg gtttattctt
     9481 tttgctttct ttacgataaa agctggcaat ctttttttct tcttgctgat aagctgtttc
     9541 tgcttttcgc tgttttttta atgactcgct tgcttgtgct tgttgtcgct tgcgaagttc
     9601 gcttcgcgta ataagcggtc ctttggccat aagccgatcc ctctactttc ctttggtcaa
     9661 tagttcccag tattgaatcg cataaatcgt ctttgcatca caaatcaact gatctttcat
     9721 cgcttgttgc gcctcttcaa gagttagatg atacaactcc agtacttcgt cttcatcttg
     9781 tgctaaagga ttctcgacct tctctacccc ttgtgcatga taaatatgaa ggacttcatt
     9841 tgcaaaacct ggtgaaagat acatcgaagt aagatggctc agcgacttcg cacgatagcc
     9901 agtttcttct tctaattctc tagctgctgt catttcggga ttttgtcctt ctcctggatc
     9961 gatttttcca gctggaatct ccaaaatcac tttttctaaa ggttttcgaa attgtttgac
    10021 aagaagcaga cgatcttgtt cgtcaaaagc aatgatcccg acaccaccag gatgaaatac
    10081 cagttctctc ttcgaagttc ctccatctgg caaacgaact tcgtctacag caacatcaat
    10141 aatttttccg ctatagattt ctttgcgact gattgttttt tcttcaaatt gctcattgtt
    10201 cagcatatct tctcctgctt tctttgtaat cataactgct tttttttcat ttttcaattt
    10261 ttctcaaaaa ctccttatta gtttaccata gataaacctt agattttctc taaatataga
    10321 aaatccttct ttagtccgat actctttcta gtagtccgta tgctaaaata agtatgtaat
    10381 cgaataacga aaagaattca atactcaacg ataaatgaca tggagggttt attatgataa
    10441 aacgaaattg gccgtggctt ctagcattgg tcttgctatt tctctttgtc cgaaacaata
    10501 gtttttcttt tgttctagga cttttattga taggtgtagt tttagttttg tggggactct
    10561 ttggttcgcg caggaaaaag ggaaaacaag aagaaattcc tgcactgact gacgaacttg
    10621 aaactcacta tgaaaaaagc ggtatgacag cgaatgaaat ttcatttttt cgacaaacaa
    10681 tgaatcaaac aaaaaatgag atcaatcaat tgcaaatgaa tatgcagcag acagccaaac
    10741 ttaaagcaat cgatctccgc catgatccag tcaaagcagc gaaagcttta ttcaaagaat
    10801 tggtgaaaga accaaaccga ctgcatgaag caagtcagtt tctttatacg catctgccga
    10861 atctagttga tttgacaaat aagtataatg agatcaatga ccatgagatc aaaaacaaac
    10921 agacctatga aaaactagaa gaaagcgcgc agatcatcga ccagctgtca gctttaatcg
    10981 cacaagatta tcaaaaattc gttgctgagg atctagatga tatcgacgta gagatatctg
    11041 ttgcgaaaca gagcttaaag cgtgataata aatatgaacc agaacaaaag gattaactct
    11101 tttgttcata aaagaagact cggaacattc agatgatgtc tggctgtttc gagtactttt
    11161 tctaaagtag agacattcat gtaagaatcg agaacagatt caacaaggag gaatttttta
    11221 tggatcataa accgactgaa acaaaagatg ttacaccagt taacgataca cttgaagatt
    11281 tattgaacaa cccgttttca actccaacag atacgctgac cacttcacaa caagcggaga
    11341 ttgatcagct ccaagagcaa cagacagctg ctcgtttaat tgacaaacta ccggcggaac
    11401 gccaagaaca agctaaacag ctagcagcaa aaatcgatgc taatgatgca cagtctgtca
    11461 tcagctacgg atcggctgcc caagcaaaat taagtgaatt ctcacaatcg atgttgaatc
    11521 atgtgcaagc acaagatatt ggtcctgtag gagattcctt aactgaactg atgtatcgtc
    11581 tccaagaagc caatcctgac gaactgcgtg ccggagaagg gaacatcttc cagcgtgtct
    11641 tcgggaaggt caaacaatcg atctatgaag tgacagcaaa atatcaaaag attggtgcac
    11701 aaattgacaa gatttcagtg aaattagata aagaaaaaga cggtctgcta aaagataatc
    11761 tgatgttaga acagctttac cagaaaaaca aagactactt tgatgcactg aatatttata
    11821 tcgctgctgg agaattgaaa atggaagaac ttcaaacaac gatcattcca gaagcgatga
    11881 aaagagcaga agaaactggc gatcagatgg atgttcagat tgccaatgat tacacgcaat
    11941 tcctagatcg tctggataaa cgtacgcatg atcttcgctt agctagacag atcacgatcc
    12001 agcaagcacc acaaatccgt ttgatccaaa acacaaatca ggccttggct gaaaagatcc
    12061 aagcatctat cgctacagcc attccgctgt ggaaaaacca agtcgtgatc gcattgacct
    12121 tgcttcgtca aaaagatgca gtaactgctc aacgccaagt ctctgaaaca acaaacgact
    12181 tattgaagaa aaattcagaa atgctgaaaa tctcagccat cgaaacagcg aaagaaaatg
    12241 aacgtggaat cgtggatatc gagacccttc aaaccactca aaatgatttg atcgaaacaa
    12301 tccaagagac attgcgtatc caaaaagaag gaaaagaaaa acgtcgtcat gcagaagtag
    12361 agcttggaca catggaagaa gatctgaaac aaaaactgtt ggatttaacc caatagatca
    12421 cctttcgctt gttactaaaa caaacgccca acaacagttt attacaactg ttgatgggcg
    12481 tttttcttat ctttcttcta acttctccat tggagtctat ttctcattca tcttgctgtg
    12541 tccatagcca tagccaaaat aaatgatcag tccaataacc attacgatac cgaacacgat
    12601 ccatgtcaca gtttgcagac gaagcatcaa tacaatacaa gtcaagatgg agacgattgg
    12661 caatagcgga acgaatggca ctttgaattc tccagctttt ggtttgccaa acatttttct
    12721 caacttcagt aagcctagac taacaagcat caaataggcg agcgccatga tattcgttag
    12781 ttccgctaat acattcaatg ggaaaaatcc ggcaaagaat acagctgcca caccagcggc
    12841 ataagtcgct tttttcggtg tacgatgctt ttgatcgatt tctgtcataa acttaggaag
    12901 taagccatcc ttgctgacag caaacagtaa acgagcaagt gaatacatca tcgagatcgt
    12961 taccgtcagt agcgttaaaa ttgcccctac agcgatcacc gcgcccactg ttccatgtcc
    13021 tacatacctc attgcaaaag ccactgggtc attgactcct agttcagtat acggaacgat
    13081 tcctgtcaag atcagcgtca cgatgatgta aaggacagta gcaatcagta tcgaaccaat
    13141 gattcctctt ggcacatctt tttgcgggtt tttgacttcc tcggctgcca ttgcaacggc
    13201 atcaaatccc aagaaagcaa aaaacacaag agctgcacca tctagcacac cggaaaaacc
    13261 aaaaggcatg aatggctgcc agttatcagg tttaacgaaa aatattccta caatgataaa
    13321 caacgcgatg ataccaaatt tgacatatac catggcatta ttcaaacgta atgctttctt
    13381 ggcttcaagc gacacgatgt aagtgacgaa tatcactacg agcaccgcaa tcaagtcaat
    13441 gtatgtacca ttttccggat tgtacccgcc agttaatgct tgtggaagtt cggcccctaa
    13501 acttttcaaa aaaccttgca tatagccaga ccatccggaa gcaacagagg aaaccgctag
    13561 taaaaattcc ccgatcaaca accaccctgt cagccaggca gcaaattcac caaatacaac
    13621 atacagatag gcatatggcc caccaatcac tggcactcgc gaagcaaatt ctgcgaaact
    13681 tagtccagac aaaatgacaa cgattgcagc taagacaaat gaaagtgaca gtgctggacc
    13741 agcattttga ttggccgcaa cccctgtcac cacaaatatt cccgtaccga tgatcgcacc
    13801 aattccaagc atgatcaaat cagacgtttt tagttctttt ttcatctcac tcgtatgatt
    13861 gagattgatc tctttcttac gaaacaaatc cattgaagtt cctccccatt ttaggttgga
    13921 aaacagcgat taaactgttt tcatataccc taaaaggata ccacctaaaa tgactaaaag
    13981 gcaaccaaca ataacataaa tgagctcttt tttcgttttt ctttcgccaa ggatgaaaat
    14041 cccacctaaa gtagagataa tgatccccat ttgtgaaagt gagaagccaa cagctaaacc
    14101 aagtgatttg actgttagaa gcatgcagat gtttcctaag ccccataata atccacagat
    14161 catgtttttc cacacaaact gatcgacttt aactttacgg aaagcaaaga agcttgcgcc
    14221 taagatcatt ccgatacttt gaggtaagat aattgacatc gcatccaaat tcgcccagtt
    14281 gacgatgatg gtatatacac cataaccaat cgtagagaag atcaatgctc taaaaccctt
    14341 accaaaatca agcattttat tatctgtatc tatttttcct tcgtcatctt gtcgggcagt
    14401 caaatacgca ccgcttacaa gaagagctaa agcaataata ccgtatacat aatctttcgt
    14461 ttgtgtccat tcatggaaga aaacggctcc cgcaatcgta ttcaaaatta attgaaaacc
    14521 agtagacaat ggtagcccta cagaaacacc catgtacttc attccatgga actgtccatt
    14581 ttgtccaaca ctccaagcta agccggataa aaatccgatc aagaaaatcc aaggggtgat
    14641 tacaggacta acaacgaaaa agacaactaa agaaaacaga aatgcaccga tcgtcattcc
    14701 tagtgtttgt tgattggcat cgccaccaat tttaccactt actaaaccaa tactacccca
    14761 tgccaacata gggactaatg ctattaaaat ttccataacc aatctccttt tatttcacat
    14821 gataagaaac ttatcagcga aaacgttaac aacacaagca ctttttttcg tttctaacga
    14881 ttttctaacg tttttcatta taaaaattta agattgggaa aaataaagtc attgttgtcc
    14941 cttttccttc agttgattca gccgtcaaat ggatacctaa tttttcggcc agattttttg
    15001 ctaaataaag tcctaatcct gttgaatgtg ttttgctaag tcgaccattt ctcccagtaa
    15061 atcccttatc aaagatccgt ttcaagtctt ccgctgggat accgatccct gaatctttaa
    15121 ctgaaaggct gactccttgc gcttcctttt ctatgctcac aatgatgttt ccgtgatccg
    15181 gcgtatattt gatggcatta ctaagcaatt ggttgaagat aaaagcgatc catttagcat
    15241 cggtcaaaac agtatgatcc tcaccaacga tctcgtagcg aatatttttt tggataaaat
    15301 aatttgcctg actgcgtatc acaggctgaa tgattccttt tagtccatat tcttggatga
    15361 ggtagtcccg cgagaaactg tcgagtcttg cataatacaa aacttgttct acgtattcat
    15421 cgatttttga taattcattt tctagtaatg tatacttttg atcgtcaata tcgaactcga
    15481 tcgaacgaac tagcaattta gaagcggaaa gtggcacttt gatttcatgg acccatgagt
    15541 caatataatc tttttggtct tgttgattac tgattgctga ttgcattact tcttggtgtt
    15601 cacggatcag ctgattgata tattcttcta ccagtttttc ttcttctgtc ctcccaccac
    15661 tcaaataatt ttgcaaaggg gaagtttttt gtatgtcggc taattttccc caccatcttc
    15721 tcttgctcaa gtaaccaccc agcagataaa aaatcagaaa gacgccctca atcaaagcta
    15781 aataagcaat tgtgctcaaa tcaactttga tgtttggcgc cagccacatc acaaagatcg
    15841 tcaatgcaat aaaaaacagc catcccaaga tcaatgtcca ctgatctttc aaatatttaa
    15901 aaaaattcat cttaaccgct catcctttca gggaacgata tatccctgtc ctacttttgt
    15961 ttcgatatat ccttggatac ctgcctgttc gatttttcgg cgtaaccgat tgatgttgac
    16021 tgtcaatgta ttgtcatcta caaatcgttc gtcatcccac agtgctcgca gcaatttttc
    16081 ccgacttaag atctttccat tgtgcttcat caaaataaat aatagacgat attcattttt
    16141 gctcaaatca atgatctctc cgttgatttc catacttccg ttgtcaacat tcaacgtaat
    16201 gccattgtgg ctcatcattt cactagataa ttctgtgtag ttgtaagaac ggcgaagtaa
    16261 tgcattgatt tttgcaatca gtacatcgat ttggaaatgt tttgtcacaa aatcatctgc
    16321 ccccatgttc atagccatga tcatatccat attcgtattc ctgctggaga taaaaatgat
    16381 cggtactttc gatacttcac gtattttttg acaccaataa tagccatcca aaacgggaag
    16441 attaatatct agtaatacaa gctgtggttc ttctctttca aagtctgaga acacttgatt
    16501 gaaatcggtc gttccaaagg tttcaaattg ccatttctca agttcctcca tgatcaattg
    16561 tcgaatgatc tcttcgtctt cgactaccat gatttttgcc ataaaaaatc cctccatctt
    16621 tcttcttgct actacaatat caaagaagcc cttgtgaaac aagttggctt acctggaaaa
    16681 tgtcatttta gcaaacgtta aaaaaaactg gaaatatgcg atttaatcgc gtttgtctct
    16741 tccaagcagc ttaaatttat tatgttattc ttaataattg gtctattatc tgttattttt
    16801 ctttcgttgt ttttgtcttt taaattttcg ataacaccaa taaccgaaaa gcgttagcac
    16861 tagcaatata cctagaagga gtataagtaa tcgataaaga tggtattttt ttgtctgttc
    16921 tatctttgaa gagacttctt ctgtaaaggg aactcgtctt cctgtcacta gtaatcggtg
    16981 agtgttcacg gtgtaaggtg tacaagtgat aagtgtgact agatcctgct tttctctaat
    17041 tgctaatgta tttgtttttg ttggaaggac aacaattttc tcaacgactt cataagcgag
    17101 catccgacca tacacttcaa gatagaatcg gtcatcttgc tccatttctt caagatccgt
    17161 aaagagtttt ttctcagcaa taccgctatg ggcggtaatc acggaatgtg tactctctcc
    17221 tccagttgga aaagaggtgc cttgcaggac tgtcgctcct ctatctaaca aaagatcatt
    17281 cgtttcatca aataaaggca agctaacatt gattttcgga atatagatgg ctccgatcat
    17341 atgttgttcg taatattctt ttcctggatt ttggacattt ctaaccgctt gctcaaaagg
    17401 atctttgact tgtcccattc ctgggattac tgatgtatga gcaagcgtct gatttttctt
    17461 ttcatgcact gctaattgtt gctcatgttt ttcttcttta tcgcgttcaa gctgcttttg
    17521 ataattctcg atccttcttt gatccaaata attgctgaga gcatctgcta aaaatggata
    17581 tgtaaaaact gcagctccgg caagaaacat cactgccata aataattgta ataaaagact
    17641 gatttgggtt tgaggtctat tacctatttt ctttttcatt ttcttcacct tcttatttaa
    17701 aaaaactgca gcaaccttta taaaaccgat tgctgcagct tatttttgtt gcttatactt
    17761 cagacttcat tttagattta cggtaccagc tgtacgctcc aatcatcaaa cttattccga
    17821 ttagtaagaa tgccaagagc ccgttcccac cagtagcagg aagtgacccg cgtttcatat
    17881 tcggtactct ttctggccct ggattttcta ctcctacgat ctgaatttct tcattatagc
    17941 ttccataaga gatgataaat tcagtaaagg ctgcctcacc tgttggaagt acataacctt
    18001 ctggcgccat ggtctctttc atttgatagg ttccataatc taacccttga acctcaaagt
    18061 atccatttcc atctgcgaca aattctgttg cttgtccgct tgttgcccac tcaattctat
    18121 ctgctcctga ttcataaaca gctaaaggcg actccccttt ataaaatatt gcgtattcag
    18181 aacggtcttc attccacaat tcgaatcttg catctttaag caattcactg cttgatgcat
    18241 catatttttc aaactttctt ccacctgttg tgactttctc tggcggctct actccttctt
    18301 cataatcaaa ttcatcattt cgtccgattt caataaccgc tgtgttgtta tggaaatcat
    18361 ccgcttctag ttctgtggaa accttcaaat tatacgtgat cgttaaatct cctccacgtg
    18421 catctgctaa tctacctaaa gtatctactt tggctactcc attttcttct gtcaaaatcg
    18481 tcacgacaaa cccgttgctt actacttcta ctatataatc ttgtcctttt gttaaaatcg
    18541 tctcgccggc tcgcacttct atttcctgat cataataagt tagaccaact gtaggcgtat
    18601 ctgtgatttt gtaatttttg ataacagcgc ttccgttttc aaggacagaa tcgatgaaat
    18661 aaggaatagg tgctgtgatc gtgtaagaag ccgtttttcc tctttctaga tcaatataag
    18721 aaatcgtttc ttgttctcca gcttcattaa taatcgttac ttgtctaagg tcgctttcct
    18781 ctacattcat ctgtttatct gcttctctga tttcgttttt cggatacaaa tgcacatctg
    18841 tattgtatgt gtctttccat gtagaaccat cccacacgcc atccaccata tcaggcatca
    18901 tcactggcat cataacaacc attggagacg cgattctgga aatatgagct ggcgaataag
    18961 tttctaagaa aagatagact ttatctctag aagaaggtgc ttcttccgtc acttggacgg
    19021 tagagaatac cgcttctcca gcttcattcg ttgtttgttt gtcaatactt gtcagtcgat
    19081 attttaaaaa ccaactttct gtgtgccgtt tttgaatcca ttcaatcgct tcggcagaat
    19141 taagaccgtc ttctcgttcc ggtgttaaag gattatctcg aatcaaatcg taatatactt
    19201 ccgttacgtc aaacaaatca aaatcgactc ctggaatatt ttcttcacca aaatccgtag
    19261 aaaccaatcc gctattcggt cgttcttccg gaacagatga gaaacctttt ttgtgtaatg
    19321 taatcgtcac ttcacttggt ttttgtgcga cttcttcggc tttggctagt tttccgttcc
    19381 cgaaaatccc tattcccgca gtaacgagta acagtacact cattcccgtg acaatcattt
    19441 ttttattcat ttttttctcc ccgttctttt tttatt
//
LOCUS       DARBFO010000045        19199 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000045 DARBFO010000000
VERSION     DARBFO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.046"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            101..2542
                     /locus_tag="SCK51_001727"
     CDS             101..2542
                     /locus_tag="SCK51_001727"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEG1252359.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2872..3576)
                     /locus_tag="SCK51_001728"
     CDS             complement(2872..3576)
                     /locus_tag="SCK51_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252360.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3763..4008)
                     /locus_tag="SCK51_001729"
     CDS             complement(3763..4008)
                     /locus_tag="SCK51_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252361.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4025..4876)
                     /locus_tag="SCK51_001730"
     CDS             complement(4025..4876)
                     /locus_tag="SCK51_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1252362.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5042..6046
                     /locus_tag="SCK51_001731"
     CDS             5042..6046
                     /locus_tag="SCK51_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEG1252363.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6287..6643)
                     /locus_tag="SCK51_001732"
     CDS             complement(6287..6643)
                     /locus_tag="SCK51_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1252364.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7011..8198
                     /gene="tuf"
                     /locus_tag="SCK51_001733"
     CDS             7011..8198
                     /gene="tuf"
                     /locus_tag="SCK51_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEG1252365.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8386..10977)
                     /gene="mprF"
                     /locus_tag="SCK51_001734"
     CDS             complement(8386..10977)
                     /gene="mprF"
                     /locus_tag="SCK51_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEG1252366.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11003..11764)
                     /locus_tag="SCK51_001735"
     CDS             complement(11003..11764)
                     /locus_tag="SCK51_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1252367.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12042..12563
                     /locus_tag="SCK51_001736"
     CDS             12042..12563
                     /locus_tag="SCK51_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEG1252368.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12622..13482
                     /locus_tag="SCK51_001737"
     CDS             12622..13482
                     /locus_tag="SCK51_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEG1252369.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13667..15982
                     /locus_tag="SCK51_001738"
     CDS             13667..15982
                     /locus_tag="SCK51_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1252370.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16164..18005
                     /locus_tag="SCK51_001739"
     CDS             16164..18005
                     /locus_tag="SCK51_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEG1252371.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18029..19138
                     /gene="rpoD"
                     /locus_tag="SCK51_001740"
     CDS             18029..19138
                     /gene="rpoD"
                     /locus_tag="SCK51_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEG1252372.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT         6226 a         3392 c         4028 g         5553 t
ORIGIN      
        1 ttaaggattg ttaaaattta atcttagaaa aaaataactg gaacaagagc tatttgtttt
       61 tacagcaaag aatggtatat ttttgtagga ggtggcttac ttgaaagaag tcgttatgat
      121 agatgctgca agaacgccga taggaaagta tcgcggaagt ttaagtccat ttactgcagt
      181 tgaattaggc acacttgtga ctaaaggatt attagataaa acaaaactga aaaaagataa
      241 aatcgatcaa gtaatatttg gaaatgtcct gcaagctggt aatggtcaaa atgttgccag
      301 acagattgct ttgaacagcg gtctccctgt agatgtccca gcaatgacga tcaatgaagt
      361 ttgcggatct ggtatgaaag cagtgatctt agcacgccag ctgatccaat taggtgaagc
      421 agaacttgtg atagccggcg gaacagagag tatgtctcaa gcacctatgc tgaaacagta
      481 tcagtcagaa acaaatgaat atggtgaacc aatttccagt atggtcaacg acggattgac
      541 tgacgcattt tcaaatgcac atatgggatt aaccgcagag aatgttgcaa cacaattttc
      601 tgtgagcaga gaagagcagg atcgctatgc cttgtcgtcc cagttgaaag cagcacatgc
      661 tgtcgaagcc ggtgtatttt ctgaggagat catcccagtc aagatttctg atgaagacgt
      721 gttatctgag gatgaagcag ttcgtggaaa tagtacattg gaaaaactgg gcacgttacg
      781 tacagtattc tcagaagaag gaactgtaac agcaggaaat gcttccccgt tgaatgacgg
      841 tgcctctgtg gtgatccttg catccaaaga atacgcagaa aataataatc tgccttattt
      901 agcaaccatc aaagaagtag cggaggtcgg tattgaccca agtatcatgg gaatcgctcc
      961 aatcaaagcc attcaaaaat tgacagatag atcaggaatg aatttatcaa caatcgatct
     1021 ttttgagatc aatgaagcat ttgcagcttc ttctatcgta gttagccaag aactgcagtt
     1081 agatgaagaa aaggtcaata tatatggagg agcaatcgca ttaggccatc caattggtgc
     1141 aagcggtgca cgtatcttga ctacattagc ttacggattg cttcgtgaac agaagagata
     1201 cggtattgct tcattatgta ttggtggagg tttaggcttg gcagtcttgt tagaagcgaa
     1261 tatggagcaa acacataagg atgtgcaaaa aaaaaagttt tatcagctta cgccatctga
     1321 acggcgcagt caattaatag aaaaaaatgt attgacacaa gaaactgcgt tgatttttca
     1381 ggaacaaaca ctttctgaag agctctccga tcacatgatc gaaaaccaag tgagtgaagt
     1441 ggaaatccca atggggatcg cgcagaattt tcaaatcaat ggcaagaaaa aatggattcc
     1501 gatggcaaca gaggaaccat ctgtcattgc agctgcaagt aatggtgcaa aaatctgtgg
     1561 aaatatctgc gcagaaacac ctcagcggct gatgcgaggg caaatcgttc tatcaggaaa
     1621 atcggagtat caagcagtga tcaatgcagt taatcatcga aaagaagagc tgattctttg
     1681 tgcaaatgaa agttatcctt caatcgtgaa acgcgggggc ggtgttcaag atatctctac
     1741 tcgggaattc atggggagtt tccatgcata cttatctatt gattttttag tggatgtaaa
     1801 agatgcaatg ggcgctaaca tgatcaactc gattcttgag agtgtggcaa acaagttacg
     1861 ggagtggttc cctgaagaag agattctatt cagtatttta agtaattttg ctacagaatc
     1921 attggcttct gcctgttgtg aaatcccatt cgaacgctta gggagaaaca aagaaatcgg
     1981 tgaacaaatt gccaaaaaaa tccaacaggc aggggaatat gcgaagcttg acccttaccg
     2041 tgcagcaacg cacaacaaag ggatcatgaa tggtatagaa gctgtagtgg cagctacagg
     2101 aaatgataca cgtgcggtta gcgcttcgat ccacgcctac gcggcaagaa atggattata
     2161 ccaaggatta acagactggc agatcaaagg ggataaacta gttggaaaac taacggtgcc
     2221 tttggctgtt gccacagtag gcggagcttc caatatcctg ccaaaagcaa aagcatcttt
     2281 agctatgtta gatattgatt cagccaagga actagcacaa gtaattgctg cagtagggtt
     2341 agctcaaaac ctggctgctt tgcgtgcatt agttacagaa ggaatccaaa aaggccacat
     2401 gggacttcaa gctcgttcat tagcgatctc aattggggct attggtgagg aaatcgagca
     2461 agttgcgaaa aaattacgtg aagcagaaaa gatgaaccag cagacagcta tacaaatatt
     2521 ggaaaaaatc cgggaaaaat aaaaaagcaa tatctttaga agaataaagc aagatttaca
     2581 gttattttat ttactgcaga tcttgctttt ttctctatca gaaaatatgt ttattattag
     2641 aattttttat agttaaggtt aggcatctta agaaatcagg caactccaag atatcttcag
     2701 gtagaagaga agtcgcctaa cattgtagga tcataggtga tttgttggac aggtcaagct
     2761 gatatttgag cctgatgtta gaaggaattt ctttttactt gctgtagaaa ttattatatg
     2821 actaaaaagg ctgggatttt aaatcccagc cttttatgac aaatagaatt tttatcgatc
     2881 agtaatcgct gtcgctgcaa attgttcgat caaaggaatc aaagtcaatt tgactttgta
     2941 tttccgttcg agtgcttttt cgataacgaa ctcattttca gtagtcatag aaatagttgc
     3001 taatttacga ggttcatcag catttttttc attaataact acttcatgtt cattttgttc
     3061 tgtgtaaaat ttaggatact cagcaacggc ttttgaaaaa tcagcaaaac ttaaagcggc
     3121 attcgtatca gttgagcgtg tattttcagt tgtgtccgct tttggttttt ccgttttttc
     3181 ttctggcaca ctgactgttt cttctgtaac tggtgtcgca acagcttgcg cagttggttc
     3241 atcgtttttc aatcgttgat cattccagta agacacgatt tcaataagaa gattgtcacg
     3301 gaatcgttcg aaaatcccgt tttctcctga acgaattctc tttaatacag tattgaagtt
     3361 gtcatcgatt ggcacgttga tccgtgtatc ttcatttgtt ttcccagcta atccgatcgt
     3421 taagatttga cggtttttgg gatcaagcat gacttgtaac ggcaaaccat aacgtcgcaa
     3481 gctctcttct tctagagaac ggaataattg tcctcttaca ccatccaatg aaggacggaa
     3541 ttgatttaat aatttttgtt tctgtttttt cttcatatgt tctctccttc gctcataccc
     3601 tttcgattat aagaaaaaaa cagccaaatg aaaaggagta atgcaaaaaa acttgcgtat
     3661 acgcaaatta caacagaaaa atttgcgtat tttcccgttt tttgaaagaa aaataaaaaa
     3721 tggaacggat tatgtatata tacataatcc gttccattct ttctattttt tcggtatcaa
     3781 ggcaaaaaca ggtagttcag gtgaataaat gacttcatgt tcgtgccagg gtttgaccat
     3841 tagcgaatga tcgaaccgtt ttttcagttc ttcgatgatt tgatcatgtg tccagtttct
     3901 tgcaggaaaa tgttggattt tctctgcatc gatcaaaaag acaaaatcgg aatgaaaagt
     3961 ctgggtaact tcttccgaga gagaattcaa gcgttcgatt ggatccataa aatcaccttt
     4021 cgacttaaaa ttgggtagta tctgggtctt ttgctttgcc gactacacca tctaactgat
     4081 cgatttgttt catttcttct tctgttaatt caaaatcaaa taattcagta ttttctttga
     4141 ttctgcttgg tgtcactgac ttaggaagag gaaggaattc gtgttgcagc gaccaacgca
     4201 aagcaacttg tgcaatcgtc ttgtcatgtg cttcagcgat ttgtttcatt tcaggcacat
     4261 cgaagatttt accagtgcct aatggactat atgcttcgag gatcatatca tgtttttttg
     4321 catactcaac tacagttgtc tgtaattcgc ctggtgcaag gaaaacttga ttgaccatcg
     4381 gcataatttt tgctgttttt gctagtgtat ctagatggtg gggtaagaaa ttgctcacac
     4441 cgattgcttt gattttacct gcttcataca gttcttcaaa tgctcgccat gtatctgcat
     4501 ttgcttgttc ccaattatca cgaaaagcaa cagggtttgg ccagtggatc aaaaacagat
     4561 ctaaataatc tgtttggagc ttcttcaatg attcttcaaa agaagacatc acaagttcat
     4621 agctgtggtt tgcattccat aattttgttg tcaagaatat ctcttctcta ggaattccac
     4681 tttccttaat cgcttggccg acgctttctt cattcttgta tccttgggca gtatcgatat
     4741 ggcgataacc tgcagccagc gcttctttaa cagaggaaac agcgacatcc ccatcaggtg
     4801 tttgccatgt gccgaatcct actaccggga tttcgtaacc atttgataga cgataagtgc
     4861 ttgttaatga attcatactt tcagctcctt ttttattcgt tcaactataa gtgtagcgca
     4921 tttttcgaaa aaaagaatcc attttgtttc tactcttgaa atttttgttt aaaagaaaat
     4981 gattttgatt ctggtatttt tgattttttt tccgtaaact aagacagaag ggaatgagaa
     5041 aatggatatc tatttaaaaa aagcaattct gcatattatt gacagagaaa ctggttctcc
     5101 agtcttttca caaaaagagc tggatctgac aaaagaatat attcgtgatt ttcttcaaaa
     5161 gaagatccaa aaaatctctt ctgctcaaac aaaaactgga caactagcag aagaaagcat
     5221 gatcgttgaa tcacttaaaa tcgctcaaga aaattttgtg gaagcaagtg aagcaatcgt
     5281 tcagcggtgg ttcgatatct accaagaaag tgaagaagca cctagtgcag atgtatttgt
     5341 tgtcttgtat gaattagata cagtgatgta cttatcttta ttgaaagtca attatcgtga
     5401 ggcttacact cactttgttg aagcagacga agcagggatc gacaaccgct tgattttgaa
     5461 tcgagcaatt ctaggggcga aaacacaaaa agcagatgaa gcattcgctg tgaatttgac
     5521 tgattggacc tacgaattaa ttgaaaaaaa atatgagttt tctggcaaaa aagagcttta
     5581 tttttctgga agagtgatcg aaagtgttcc tgcaccttct ttagaagaaa atgtaaaagt
     5641 gatccaaaaa gtagccaagc atcttggaaa aaaatttgag acagaagaat ttgatatcat
     5701 ggctgatttg aaagaagctg tctatgatac gatagaagaa aaaggtcggt tggatcatga
     5761 gatgattgcg gaaaaagtct tcaaggaaaa catcacggca aaaatggctt ttaaagaaga
     5821 agtacaagag caaggatttg tcccagaagc acctcctgta aaagaagtac aagaaatttc
     5881 tgagaaaaaa tttggaaaac aaaaattcaa gctatcaaac gggatcgaac tgatcgtacc
     5941 agtagatgta tatcgcaacc cagatttgat tgaatttgtg aataatccgg atggtacgat
     6001 atccgtgatg atcaaaaatg tggatgaagt attgaatcgt ttataaaaat gactagaata
     6061 ttaggtgttc aagtaccagt gatcagttaa atgaattgtg tcaagagaag gatcggaaaa
     6121 gacgaatata acaaaaaaag agcgtgagac aaaagtaaag aacacttttg tctcacgctc
     6181 taaacacgta taaacggcgg aagcagaagc aactccttct tatttcttgg agttaaacgc
     6241 ttctgtccca gcctcttttt atctaataaa attttaagac catttactaa ttatcgattt
     6301 tttctgccgc atttttaggc acttcagaag cctttatttc ttcccagctt aatacacctt
     6361 tacgaggatt gactttgagt ttaagatacg cattcatctt taatggatgt tctctttctt
     6421 cgcgaagcgt cacctgtttt ttttcaccat ctttattata agccgcttgc ttgtaggtat
     6481 aagtcgtgaa tttctctccg ttatctgctt gggtagctga catttcgcca ttcgtcgtga
     6541 tttttgtata ataagcttcg ccgccgtaaa aataatgata agctgcaaaa cttccgccga
     6601 gaaatatgcc taaaacgaca agtacgccaa ttaatttttt catgtacttc acctctttcc
     6661 ttattattaa cgaaatagaa gatttcgtct attgaaagtt gttggcaaca gctgaatttt
     6721 aaacttttcc tcattttgtt cttattgctt caaaagaaag ttaaggacta tcttgaagtt
     6781 tttctcaaaa aacacatttt tttatttata aaaggtactt ttttcgcgta taagacagac
     6841 gagtgctctt agttgacttt gaccaatatt gacgtataat aagcttagtt cgaatcttac
     6901 aaaaagtaag tcggacgtat tctttttgat ttagtcaaaa aaagactata ataaaaaggt
     6961 gagagctgaa atttttcagt gatctatatt atttaggagg tttgtaaatt atggcaaaag
     7021 aacattatga cagaagtaaa ccacacgtta acattggtac gattggacac gttgaccatg
     7081 ggaaaactac tttaactgct gcgatcacta cagtattagg caaaaaaggc ctagcaaatc
     7141 ctcaagacta tgctagtatt gatgcagctc cagaagaacg tgaacgtggg attacgatca
     7201 ataccgcaca cgtagaatat gaaacagaaa aacgccacta cgcgcatatt gatgcaccag
     7261 gacacgcgga ctatgtgaaa aacatgatta caggggcagc acaaatggat ggtgcaatct
     7321 tagttgtttc tgcaactgac ggtccgatgc ctcaaacacg tgaacacatt ttattgtcac
     7381 gccaagttgg tgtaaaatac ctgattgttt tcttgaacaa agttgattta gtcgatgatg
     7441 aagaattgat cgatttggta gaaatggaag ttcgcgagtt attgagcgaa tatggtttcc
     7501 caggcgatga cactcctgtg atcaaaggtt ccgcattaaa agcattgcaa ggcgatccag
     7561 atgctgaagc tgctattatg gaattgatgg atacagtaga tgaatatatc ccaacaccag
     7621 aacgtgatac agataaacca ttactattgc cagtggaaga cgtcttctca attacaggtc
     7681 gaggaactgt tgcctcaggt cgtattgatc gtggtgctgt tcgtgtcggt gatgaggtag
     7741 agatcgtagg gatcaaacct gaaacacaaa aagcagttgt aacaggtgta gaaatgttcc
     7801 gtaaaacgtt agattacggg gaagctgggg ataacgtagg cgtgttgtta cgggggatcc
     7861 aacgtgacga tatcgaacgt ggacaagtac ttgctaaacc aggttccatt actccacata
     7921 caaaattcaa agcagaagtg tacgtgttga caaaagaaga aggtggacgt catactccat
     7981 tcttcaacaa ctatcgtcca cagttctact tccgcacaac tgatgttaca ggaacaatca
     8041 cattgccaga agatacagaa atggtcatgc ctggtgacaa cgtgacgatt gatgttgact
     8101 tgatccatcc aatcgctgtt gaaaacggaa cgacattctc aattcgtgaa ggcggacgga
     8161 cagtaggttc aggtatcgtt acagagatcg aagcataaga aacgcgactc gaatattttg
     8221 gtaatgaaaa atgtttaaaa taaaagagcg gtccaaacag gatttctgtt tggaccgctc
     8281 tttgttcttc aattgtataa gaagagattg tagaagcatg aactttcgtt ttacttcagt
     8341 ctcttttatt catcatgaga gtgttcatct atcggtatgc agtttttaat actttcttcg
     8401 tatccatttt ttcaaaccac ttattctacc ttgtgtctgc tcaactggcg cattatcaat
     8461 aatcagcaaa gcaatcataa catatgcaat ccaactatct ctagaatata gagtataacg
     8521 tgagacccag tttgtagcgt acttgctttt atattctctt aatccatgga aagaataaaa
     8581 atgagaacca aactcgtaaa ccaagtatgc aatcctttcc tgtaaaaaac tctttctgga
     8641 agttccgaca tttgaaagag gggccatccc taaattgaac cattggatat gttcttcttt
     8701 catataggca aacaaatgca agaacaagaa atccatactg ccagaaggtg cttttttaga
     8761 atcatatcgc atcagatcaa tcgttcctac tttattgttc gtgtaagtag gcatgatcgt
     8821 tgcaaaagcg acaatttctt cttgggtatt tttgacaact gcaataggcg ctctttgtag
     8881 ataatcttca gagaaaaagc ctaaagaaaa ccctttttcc tttcgtgaac ccaaccaatt
     8941 atcagagata tttttgaaag tactcatttg ctcagtagaa aatggtggtt gtaagacatc
     9001 aaatgtaaat ccttctcgtt cgatccgatt caagacagca cgagtgccct tcattttttt
     9061 ccctgaagtt gtaaaactat tcaggtctac ataagcttct tcccccatct taatgaaatc
     9121 gtagccgaat tcatgcaaaa tcataacaat ctcttcgctt gtttcgtaga aaactggtaa
     9181 atagcatagt cgatctgttt cttcgataaa agcttcgatt gcttctggaa aatcttcttt
     9241 cttaccagaa ggatcaccca ttacaataca tttattattg aagcaagcga attggagaag
     9301 aacagttggt tcaccgtctt tttcataggc aaacatccgc ttatctttta agaagatcaa
     9361 ttgactatcg gagtttcctc cataagttgt aaggatcttc agggcctttt cttcattaaa
     9421 ggcttcgcca atctgtttct tttctccttg aaggaaatga acaaatagaa cgatcatgaa
     9481 actaacagcg atgatggcca acaatcccga aaaccaaatc ttttcagaag gaaataagaa
     9541 aaaggaaata aaatggtgac gccgatgagg gaaatccggc agattgtata ccccaataac
     9601 gatgtaaagc aaggacagtg caccaataat gattccatcg atcgtacgcc actcccatga
     9661 atagatcaat tgttcccgaa ataattcgtt tttagaagca agaatgatca gaagcagaat
     9721 agacaagaaa accaccggtg tgaaactaaa atcacttaat aaaacataaa gtaaagctag
     9781 aacaatcaag aagattgtag gcaaatatgc tcgtttcaca cgtgctgcga tccctctgcc
     9841 cataatgatt aaaagaaatc ccaatagtat actaggaaat tggataataa aatgaaattt
     9901 caatgggttc aaactatgaa gccagcgaaa ctctgtaaaa gcttgcggga tagtggcaga
     9961 aagtactagc atgattccag aaaagtataa taagacaacc acgattttat gcgcaatttc
    10021 tgttgctaat tgtttaggga taccagaata acgctgatca aaagtggaac cgatattttt
    10081 gaagaaaaag acgattccaa tcaaaaacgg aatgatgtag tagaacaggc gatagagcaa
    10141 gatccacaca acgactattt ctcttggcac accaatagcg gacaacccga taatcatcat
    10201 tacatcgaaa ctcccaattt ctcctggaat catagacacg attcctatca ctgaagcagc
    10261 aatgaataat ggaattgttt gccacagatc aatccggatt tccattaaat aaccaacaga
    10321 aaggaaacta aacaggacac cggtccattc caacaatgaa actagcaata gctgtccttg
    10381 agttttaagt gaggtaccac caaggaatcc tttctttttg aaagtagtaa ataaaaagac
    10441 gacaggaaaa tatagaccgc cgccgatcaa ccatatccaa tattgcttca aaaaaggagc
    10501 aactggcgtg aagactacaa gtaaaaaaga aagcagactg taaatagaaa gtccagccat
    10561 caaaaacaag aaaactttcg ataatgcttg gatcacgttt ctgctctctt tcttttgtcc
    10621 atatagttca gagcgaagcc ccacactaac aaacccgccg aaaccggcaa tattgttgat
    10681 cgtattgatc agccagcttg tctcaaacag gtaacgagga ttctgctttt gttcaagcat
    10741 ccgattcaaa ataatatcat aaccaaccat aggtaacaca gagataagac caatcaatag
    10801 cattagtccg cttttccata caggtatagt ggcaaacgtt tctcctaatt gctggataga
    10861 tagagtttta ccgatggaca tcaattcacc gacaataatg acaatgacag aaatcaaaaa
    10921 gattgttttg aacagtccta ggtggttttt cagccattgt atggtatttt ttaacaaata
    10981 aatcaccttc tattcctaat agttatgttt cagaaagaaa ttgaagaact tctcgattga
    11041 agcgttcagg atcttttcta gctaattcat gtccttgttc tttcacaagc acaaaggaag
    11101 cttttggtat agttttagct atatacaaag aatgttttag cttgattaca tctttttttc
    11161 ccacgatgat caaagtgggg gagttgattt tttttaaatc attttctgta agcccgatgt
    11221 catgcagcaa aagtttgata actaaaagat ttttgcgaag acttgggcga aataaagaga
    11281 gtaaccatac ccatgcataa tgaatattac tgatgactct ggcagaaaaa cgaacgcctt
    11341 taacaagcgt gtttccagaa tttaaaatca atcgatgaac ttttttaggg aagctgctgg
    11401 caaataccag tgctagattt gctccatcac taaatccaag aaaatctgcc tgatcgattt
    11461 tttccagcaa catgatcgta ttcagatcct cggccattaa ctgaaaattc aacatagatg
    11521 cttcgtttgt cgaacgacca tgtcctcggc tatctactag atacacggta tagtagcgtt
    11581 cgagtacagg gacctgttct gaaaaaaagc ggccgctgcc gtcattgcca tgcagtaaaa
    11641 agagagggaa gccttgaccg ctcttttcat agtaaatttc gctcccatct ggcatttttg
    11701 catatttctt ttcttttttc acataatcac ctactgtgta aagtataacg attatatcag
    11761 tcattgggca attgttttta ctgaaaaaca acgatcattt tcgtaagatg tcaaaaaata
    11821 caggaaaatt cattttttct taaggcgtac acagaaatca aggaagaaat agccaagaag
    11881 ataaagaaat agtaagataa aaaaagatgt caaaataact aggtgatgaa atgaagcgaa
    11941 aacgattatc tattatccgt attctttttt acatgatcat tctgacggtt attggtgcgg
    12001 tgttgttttt atgaggaaga acttatcgtt cggtaaaacg agtggaaagc tatcaaatag
    12061 aaataacaac agctgcaaaa aattatcata tggaaaacta cgagaatttg atcaaggcga
    12121 taatattgac cgaatcaaaa ggaaaaggga cagatctcat gcagagctcg gaaagtctgc
    12181 atggagaagc tgagatcatt gataatccag ctgacagtat tgatcaaggt gtcaaatatt
    12241 tggcggaaat gatagtagaa gcaaagaaac aaggatgcga tttatggaca gctgtccaag
    12301 catataattt tgggaaagat tatattgcct atgtaaaaaa tcatggcgga gaaaacacag
    12361 tagaattagc agaagcgtat tctaaagatg tgctgagccc acttttagga aatgaggaag
    12421 ggaaacaata ccgatattgg cggatccagt ccttgcttta caatggcggc tatctctatc
    12481 ataatggagg taacatgttt tatcctgata ttgtcaaaat gaatgaaaaa ataatagaga
    12541 cctatgaaaa agtctttaag taaaatagca ggaaatgttc aatcatgaat aaaattaaat
    12601 gaaagaagga gtgcgcaaat agtggaaaaa aggcaaaaaa tggaaaatca agaagtgggg
    12661 tacacaacca aagactggat gttacggttt gtgaagggaa tgtttatcgg ttcaggattt
    12721 atcttgccag gagtcagcgg tggtgcatta gcagctgtct ttggcatgta tgagcggctg
    12781 atttcttttt tagcccacct tacaaaaaat ttcaaagaaa atgttttgtt ttttctgcct
    12841 gttggtctag gcggagtgac aggcatcttt ctactttctt ttttagtcag ttttttatta
    12901 ggtgcgtatg aaacaacgat attatggttt tttgtcggat gtatcgctgg aactgttcct
    12961 gcgttatgga aagaagcagg gaaaaaagga agagatactt ctgatgtgat catcatggtt
    13021 gttacttttg ttctggctta tttgttttta ttatacggcg ctcaattatt tgacgggcaa
    13081 gtcgatcaga attttggaac atggctgatt gccggcggat tgattggttt gggtatgatc
    13141 gtcccaggat tgagcccttc taattttttg gtgtatatgg gattgtataa agcgatggct
    13201 gacggattta aagaagtacg acttgaagtg attgtgccga tcggaattgg tgggattctt
    13261 tgtgttcttg gattgtcaaa aatcatggat tacattttct caaaggcata tagcaagcta
    13321 ttccatttca ttttaggtgt agtttttgct tcaacgatta tgatcattcc taccgattac
    13381 gcgggattgg gagttgctgg ggtactagct tgtcctgttc tttttatagc cggtacagca
    13441 ctaggttggt ggatgagccg tttggaggaa caatataagt aaagatagat tgataataaa
    13501 aacagcacac gggtgattca aacaaaaagg ttggcatctt ttttgtaaaa tcattcgtgt
    13561 gtttttctat aaattttata aaaacaagtt gtttttaatc ttggaacacg ctagacttaa
    13621 acacgtgatt atttttgaaa gttggcggaa aatggagaaa aagaaaatga atggacttac
    13681 acagcaagag gtagttcaga aaatggcttc tggccagcaa aatgactacc aggaagatgc
    13741 atcaaaaagt acaaaacaaa tattcagcga taatatattg acgttattca attttctgaa
    13801 ctttggtatt gggatttgtc tgttatttgt aggtgcgtat tccaacatgg cgtatttagc
    13861 aattattgtc gtcaatatcg ttattggaat ttatcaagaa atacatgcaa gaaatcttgt
    13921 acggaaatta tcgattgttt cccaatcaga agtactagtg atccgggacg gtaaagaaca
    13981 atcgatttct actaaagagc tggtattagg agatttggta aaaatcggag caggagaaca
    14041 gataccagcc gatttacagg ttatatctgg acgggcagaa gcaaatgaag ctcttttgac
    14101 aggcgaatct gatttgattg tcaaagaaac tggagctgaa ttgctttctg gaagcttttt
    14161 gacaagcggt tctgtcatgg ctgaagttat ccgagtaggt gcagataatt atgctgtccg
    14221 cttgactagc gaggcaaaga ctcacaaagc tattcgttct gaacttgtag attcgattcg
    14281 caaagtatcc aaattcacta gtttcgtgat tattcctttg gggatcatac tatttttgga
    14341 agcactgttc atccgcgatg cttctatcaa aatttctgtt attgcatctg cagcagcttt
    14401 gttaggcatg cttccaaagg gtttggtatt gctaataagt attgcattaa cgacaggggt
    14461 aaccaaactg gcaaaaaaac gaatccttgt tcaagacatg tattcaattg aaacgctggc
    14521 ccatgtcgat acattatgct tggataaaac agggaccatt actgaaggga aaatgagtat
    14581 ccagcgggta gaagcactac atccgctgta cgagaaagcc atcccagaaa ttatggggac
    14641 gtatctggca gaaagcagtg ataacaacat cactatgcgg gctatgagag attattttga
    14701 gctttctaat cggtatgatg tgacaaacaa agtcgcattt tcttctgatc gcaaatgggg
    14761 cgcgatggaa tttccagaac tgggagtcgt gtacgtagga gcgcctgaac gtttggtgga
    14821 agaagatcgg cttccggaca gtctaaaaac agcccaagcg aatggctatc gcgtacttat
    14881 gttggggatc gctccaaatc agcaattgaa cgaagaaaaa ccagcatttg ttgagccggt
    14941 tgctctgttt gaaatcgatg atccaatccg aaaaaacgct caagaaacac tagcttatct
    15001 tcatgaggaa ggtgtggatc tgaaagtgat ttctggagac aatcctttta ctgtctccaa
    15061 tatcgctaga cgtgcaggat taccgaatta tgatgcgtat gtcgatcttt ctcaaattac
    15121 tgaagaaatg gaagtgcgag aagctgctca tcgctatacg gtttttggac gagtgagtcc
    15181 gcagcaaaag aaattgctgg taaatgaatt gaaagaaagc ggcagaacgg ttgcaatgac
    15241 aggcgacggt gtgaatgatg ttttggcgtt aagagaagca gattgcagca ttgctatggc
    15301 agaaggtgat ggagcaacac gtcagattgc gaatctggta ttgttggact ctgattttac
    15361 tacattgcca gaagtcctgt ttgaaggacg ccgagtggtc aacaatgtga cgaaagtctc
    15421 aggcatcttt tttatcaaaa cgatttattc atttatttta tcgattatat gtgccgttac
    15481 agcaattgga tttccgttta tccctatcca aatcacgctg attgatttag cgattgaagg
    15541 gtatccttcc ttctttttat cttttgaaca agataaaaga aaaatcactt accgttattt
    15601 gcctacagca cttattaatg ctttaccaaa tgccttgctt gtagtagtga atatcattgc
    15661 ggtgtatcta cttggaaaaa cattcgcatg gggaaatgct gaaactacta ctttgatgta
    15721 ttacttgctg attgggatca gttgcatggc agttgtaaaa gcttgttatc ccttcaaccc
    15781 attgcgcatc ttcctagcag taacgacgat cgtagggata tatgtagctg caatgctgtt
    15841 ccatggaatt ttggaagtaa acttagggtt gggagagagc gtaggatact tctttgtatt
    15901 catgctcgta aatattgttc ttcgagtatt gtactggaag gctgatattc aaaatcagtt
    15961 actgaaaaga gtagcaaatt gatgcttaat gagatagaaa tgtgttatac ttgaaaaagc
    16021 aatgaaatat gatacaatca aacatgtata attgtgtcct ttaaaatcgc actgatttca
    16081 gttagtgcga ttttttaccg gttattctcc tgaaatatct tctgttaaac ccaaaagata
    16141 tcaatagagc agggggtgaa aaaatggctc aacggattcc tcaagaggtt atcgaaacag
    16201 tacgcagtca aacgaatatc gttgaggtga ttggacaata tgtccagttg aaaaagtctg
    16261 gtaaaaatta cttggggctg tgtccttttc atgaagaacg tacaccgtct ttttctgttg
    16321 cagaagacaa gcagattttt cattgctttg gttgtggtaa aggcggaaac gtgtttactt
    16381 ttcttcaaga actagaaggt ctaagttttc cagaagcagt ggtaaaagtg gcagacttcg
    16441 aacagatacc gttagaagat caatggcgtc agcgtactat agctgtccaa aatcctgaat
    16501 ctgctacggg gaaactgatt gctgctcatg aaaaagcagc ggaaatctat catcacatgc
    16561 tattgcatac aaaagctgga cagccagctt tagaatattt gcaaagtcgt gggttgtctt
    16621 tagaattaat tgaagaattc aacataggtt ttgcacctaa tgagcgttct tttcttcagc
    16681 aagtattcaa gaatgaatca atttcaaatg aattgttaga acgctctgga ttgtttgtca
    16741 cgcatgatga tggccgtatg tctgatcgtt tttatcaaag agtgatgttt ccacttcgta
    16801 attctcaagg ccagacgatc ggtttttcag gacgctggct tgcacctgaa aaagggcagg
    16861 aaaaagatca gcccaaatac ttgaatagtc cagaaacaga actgttcaat aaaagacaag
    16921 ttttatttaa tcttgataaa gcaagaagcg atatccggaa aaacggagaa attttgttgt
    16981 tcgaagggtt tatggatgtg attgctgcat ggcaggctgg tatcaaaact ggattagctt
    17041 ctatggggac aagtctaaca gctcaacaga tccaacaaat ggaaaagctg acgaaagaat
    17101 tggtcttttg ctatgacgga gacaacgcag gttttgaagc aaccaatcga gggattgaat
    17161 tgcttcggca aaacagtcga ttagcacttt ctgtcgtagt cgttccagaa aagcttgatc
    17221 ctgatgaata tctccgtaag tatggagaag catctttcca agaattagtg gctcatgggc
    17281 gcgaaacagt ctttactttt aaatcacgtt atttaaaaca agggaaaaat ttagataatg
    17341 aaaaagacaa gatcgaatac ctcgaagaat tactagttga acttagccga gttgtttcac
    17401 caatcgaaca agatatgtat ctatctcaac tgtcaacaga gtttcagatc ggcagagaga
    17461 cgatacaaaa acaactgcgc gatcttcgaa ggacaaatca gcaaaatcgt caagaacctc
    17521 aggaaagtca acacactcag gttacgagac aagaaactgc acggaaaaag agacctttga
    17581 cgcaagtaga aaaggcagag cagattctat tatatcgtgc atttaaagaa gtttctgtcc
    17641 gtaatatttt aaaagaacga gaatttcagt ttattcatga tgcatatgca gaagtttatt
    17701 ttttattcga tgcatatgtc tcacaacatt atgaattcaa ccttgcagag tttttagact
    17761 ttttgcaaga tgaaaattta aggcggttgg tggtggaaat cgaatattta ccggtggcag
    17821 aagaaagttc gccacgagaa atccaagact tgctagatgt gatcagcaaa tctggcttag
    17881 ctgatgaaat aacattaaaa aaacaaatgc agcaagaagc cagacgaact ggaaacaaac
    17941 agcttgagct tgaattgacg attgaaatca tcaatttaac gaaacagtta aaacaagcca
    18001 aatagctact caaggggggc cttcttttat ggcaaatgga acagataaaa aaacgtatga
    18061 agcagcagta gcagctttca tcaaagaaaa caaacccaaa cgacaagttg tatatgatga
    18121 tttatccaat cagcttgcta caccattcac tttgaatgca gatgaaatgg acaaattgat
    18181 ccaaaagatt gaagacgcag gtatcagtgt ggtggatgaa aatggtgatc catcaattca
    18241 cagcttgaag agtgctgaga aaaaagctga acaagcaaaa acagaagatc tttctgcgcc
    18301 aaccggtgta aaaatcaatg acccggttcg tatgtatctg aaagaaattg gccgtgtctc
    18361 ccttttaact gcggaagaag aagtagcgtt agcattgaaa atcgaagaag gcgaccaaga
    18421 agcgaaacag cgtctggcag aagcaaacct tcgtttagta gtaagtatcg ctaaacgtta
    18481 tgtaggacga ggcatgcagt tccttgactt gatccaagaa ggaaacatgg gactgatgaa
    18541 ggcagttgaa aaatttgact accgcaaagg atttaagttt tcaacctatg ctacatggtg
    18601 gattcgtcaa gccatcactc gagcaatcgc tgaccaagca cgtacgatcc gtattccagt
    18661 tcatatggtt gaaacaatta ataaattgat acgtatccaa cgtcaattgc tgcaagacct
    18721 tggaagagaa cctacgccag aagaaatcgg tgcggaaatg gatcttccaa cagaaaaagt
    18781 ccgagaaatc ttgaaaatcg cgcaagaacc tgtttccctt gaaacgccaa tcggtgaaga
    18841 agatgactct cacttaggtg atttcataga agaccaggaa gcaacaagtc cagcagaaca
    18901 cgcagcttat gagttactaa aagaacaatt ggaagatgta ctagatacat tgactgatcg
    18961 ggaagagaat gtccttcgct tacgttttgg tttagatgat ggccggacga gaacattgga
    19021 agaagtaggg aaagtatttg gtgttactcg ggaacgtatt cgtcagatcg aggctaaagc
    19081 attaagaaaa ttgcgccatc cttctcgttc aaaacaatta aaagatttct tagaataagc
    19141 gtggactatt gaccagaaga gaaatcagta aaactgtttg gtttatttta aaagaaaaa
//
LOCUS       DARBFO010000046        18740 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000046 DARBFO010000000
VERSION     DARBFO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.047"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            256..531
                     /locus_tag="SCK51_001741"
     CDS             256..531
                     /locus_tag="SCK51_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252373.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="SCK51_001742"
     CDS             544..2616
                     /locus_tag="SCK51_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEG1252374.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2781..4667
                     /locus_tag="SCK51_001743"
     CDS             2781..4667
                     /locus_tag="SCK51_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1252375.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="SCK51_001744"
     CDS             4679..5626
                     /locus_tag="SCK51_001744"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEG1252376.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="SCK51_001745"
     CDS             5646..6173
                     /locus_tag="SCK51_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG1252377.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="SCK51_001746"
     CDS             complement(6236..6889)
                     /locus_tag="SCK51_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEG1252378.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="SCK51_001747"
     CDS             7021..7863
                     /locus_tag="SCK51_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1252379.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="SCK51_001748"
     CDS             8021..8917
                     /locus_tag="SCK51_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG1252380.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="SCK51_001749"
     CDS             8920..9633
                     /locus_tag="SCK51_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEG1252381.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="SCK51_001750"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="SCK51_001750"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG1252382.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="SCK51_001751"
     CDS             10168..10395
                     /locus_tag="SCK51_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEG1252383.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="SCK51_001752"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="SCK51_001752"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG1252384.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11681)
                     /locus_tag="SCK51_001753"
     CDS             complement(11181..11681)
                     /locus_tag="SCK51_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEG1252385.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="SCK51_001754"
     CDS             complement(11960..12358)
                     /locus_tag="SCK51_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1252386.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="SCK51_001755"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="SCK51_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEG1252387.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="SCK51_001756"
     CDS             13471..14238
                     /locus_tag="SCK51_001756"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEG1252388.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="SCK51_001757"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="SCK51_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEG1252389.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="SCK51_001758"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="SCK51_001758"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEG1252390.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="SCK51_001759"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="SCK51_001759"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEG1252391.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5092 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt
//
LOCUS       DARBFO010000047        18681 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000047 DARBFO010000000
VERSION     DARBFO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.048"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            202..1077
                     /locus_tag="SCK51_001760"
     CDS             202..1077
                     /locus_tag="SCK51_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252392.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="SCK51_001761"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="SCK51_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="SCK51_001762"
     CDS             complement(1642..2973)
                     /locus_tag="SCK51_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1252393.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="SCK51_001763"
     CDS             complement(3122..3835)
                     /locus_tag="SCK51_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1252394.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="SCK51_001764"
     CDS             4010..5434
                     /locus_tag="SCK51_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1252395.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="SCK51_001765"
     CDS             5733..6440
                     /locus_tag="SCK51_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEG1252396.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="SCK51_001766"
     CDS             6427..6750
                     /locus_tag="SCK51_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEG1252397.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="SCK51_001767"
     CDS             complement(6839..7876)
                     /locus_tag="SCK51_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1252398.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="SCK51_001768"
     CDS             complement(8197..9180)
                     /locus_tag="SCK51_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEG1252399.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="SCK51_001769"
     CDS             complement(9595..10365)
                     /locus_tag="SCK51_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1252400.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="SCK51_001770"
     CDS             complement(10358..11293)
                     /locus_tag="SCK51_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1252401.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="SCK51_001771"
     CDS             complement(11609..13258)
                     /locus_tag="SCK51_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEG1252402.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="SCK51_001772"
     CDS             complement(13375..15978)
                     /locus_tag="SCK51_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEG1252403.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="SCK51_001773"
     CDS             complement(15990..17039)
                     /locus_tag="SCK51_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEG1252404.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="SCK51_001774"
     CDS             complement(17065..17379)
                     /locus_tag="SCK51_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEG1252405.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="SCK51_001775"
     CDS             complement(17395..17841)
                     /locus_tag="SCK51_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1252406.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..>18681)
                     /locus_tag="SCK51_001776"
     CDS             complement(17842..>18681)
                     /locus_tag="SCK51_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1252407.1"
                     /translation="KRSFMISVDLLEEIEERFCIHFNEDEIAYVTLHIQSLLDRYKPD
                     KTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRKVTDELVISTIPIE
                     NTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCFFTHQIKDNQVNVL
                     NIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFIKKPVICVYVNPEG
                     IDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLKTSSSFEKLVYILK
                     EGL"
BASE COUNT         5721 a         3767 c         3061 g         6132 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt t
//
LOCUS       DARBFO010000048        18223 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000048 DARBFO010000000
VERSION     DARBFO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.049"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..152
                     /locus_tag="SCK51_001777"
     CDS             <1..152
                     /locus_tag="SCK51_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1252408.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="SCK51_001778"
     CDS             complement(230..763)
                     /locus_tag="SCK51_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEG1252409.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="SCK51_001779"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="SCK51_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEG1252410.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="SCK51_001780"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="SCK51_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEG1252411.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="SCK51_001781"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="SCK51_001781"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEG1252412.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="SCK51_001782"
     CDS             complement(4488..5066)
                     /locus_tag="SCK51_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEG1252413.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="SCK51_001783"
     CDS             5388..5858
                     /locus_tag="SCK51_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEG1252414.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="SCK51_001784"
     CDS             complement(5966..7192)
                     /locus_tag="SCK51_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG1252415.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="SCK51_001785"
     CDS             complement(7946..8146)
                     /locus_tag="SCK51_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1252416.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="SCK51_001786"
     CDS             complement(8280..8888)
                     /locus_tag="SCK51_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEG1252417.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="SCK51_001787"
     CDS             complement(8973..9443)
                     /locus_tag="SCK51_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEG1252418.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="SCK51_001788"
     CDS             complement(9491..10405)
                     /locus_tag="SCK51_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG1252419.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="SCK51_001789"
     CDS             10551..11354
                     /locus_tag="SCK51_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEG1252420.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="SCK51_001790"
     CDS             11532..12479
                     /locus_tag="SCK51_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEG1252421.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="SCK51_001791"
     CDS             complement(12547..12840)
                     /locus_tag="SCK51_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEG1252422.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="SCK51_001792"
     CDS             complement(12868..13533)
                     /locus_tag="SCK51_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEG1252423.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="SCK51_001793"
     CDS             complement(13673..14305)
                     /locus_tag="SCK51_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1252424.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="SCK51_001794"
     CDS             complement(14312..15379)
                     /locus_tag="SCK51_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1252425.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="SCK51_001795"
     CDS             complement(15376..16107)
                     /locus_tag="SCK51_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEG1252426.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="SCK51_001796"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="SCK51_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEG1252427.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="SCK51_001797"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="SCK51_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEG1252428.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         5416 a         3690 c         2911 g         6206 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat ttt
//
LOCUS       DARBFO010000049        17894 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000049 DARBFO010000000
VERSION     DARBFO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.050"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(50..1516)
                     /locus_tag="SCK51_001798"
     CDS             complement(50..1516)
                     /locus_tag="SCK51_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEG1252429.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1903..2499
                     /locus_tag="SCK51_001799"
     CDS             1903..2499
                     /locus_tag="SCK51_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG1252430.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2557..3039)
                     /locus_tag="SCK51_001800"
     CDS             complement(2557..3039)
                     /locus_tag="SCK51_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252431.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3145..3342)
                     /locus_tag="SCK51_001801"
     CDS             complement(3145..3342)
                     /locus_tag="SCK51_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252432.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3637..4395
                     /locus_tag="SCK51_001802"
     CDS             3637..4395
                     /locus_tag="SCK51_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEG1252433.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4455..4883
                     /locus_tag="SCK51_001803"
     CDS             4455..4883
                     /locus_tag="SCK51_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252434.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4929..5001
                     /locus_tag="SCK51_001804"
     tRNA            4929..5001
                     /locus_tag="SCK51_001804"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4962..4964,aa:Gln,seq:ctg)
     gene            complement(5151..5522)
                     /locus_tag="SCK51_001805"
     CDS             complement(5151..5522)
                     /locus_tag="SCK51_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1252435.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5737..5922
                     /locus_tag="SCK51_001806"
     CDS             5737..5922
                     /locus_tag="SCK51_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252436.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6005..6193)
                     /locus_tag="SCK51_001807"
     CDS             complement(6005..6193)
                     /locus_tag="SCK51_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252437.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6217..6480)
                     /locus_tag="SCK51_001808"
     CDS             complement(6217..6480)
                     /locus_tag="SCK51_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252438.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6638..6895
                     /locus_tag="SCK51_001809"
     CDS             6638..6895
                     /locus_tag="SCK51_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEG1252439.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6966..7157)
                     /locus_tag="SCK51_001810"
     CDS             complement(6966..7157)
                     /locus_tag="SCK51_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252440.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7298..7618)
                     /locus_tag="SCK51_001811"
     CDS             complement(7298..7618)
                     /locus_tag="SCK51_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252441.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7641..8075)
                     /locus_tag="SCK51_001812"
     CDS             complement(7641..8075)
                     /locus_tag="SCK51_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1252442.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8410..9048
                     /locus_tag="SCK51_001813"
     CDS             8410..9048
                     /locus_tag="SCK51_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1252443.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(9372..9455)
                     /locus_tag="SCK51_001814"
     tRNA            complement(9372..9455)
                     /locus_tag="SCK51_001814"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9419..9421),aa:Leu,seq:cag)
     gene            9983..10483
                     /locus_tag="SCK51_001815"
     CDS             9983..10483
                     /locus_tag="SCK51_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1252444.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(10673..11017)
                     /locus_tag="SCK51_001816"
     CDS             complement(10673..11017)
                     /locus_tag="SCK51_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEG1252445.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(11171..12091)
                     /locus_tag="SCK51_001817"
     CDS             complement(11171..12091)
                     /locus_tag="SCK51_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1252446.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            12278..12769
                     /locus_tag="SCK51_001818"
     CDS             12278..12769
                     /locus_tag="SCK51_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEG1252447.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(12793..15168)
                     /locus_tag="SCK51_001819"
     CDS             complement(12793..15168)
                     /locus_tag="SCK51_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEG1252448.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            15494..16120
                     /locus_tag="SCK51_001820"
     CDS             15494..16120
                     /locus_tag="SCK51_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG1252449.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            16143..16847
                     /locus_tag="SCK51_001821"
     CDS             16143..16847
                     /locus_tag="SCK51_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1252450.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            17039..17872
                     /locus_tag="SCK51_001822"
     CDS             17039..17872
                     /locus_tag="SCK51_001822"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEG1252451.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         5557 a         3490 c         3015 g         5832 t
ORIGIN      
        1 aaaacaggaa ctgctgcaaa taagcaattc ctgtttttat gttcattgat taatgaatag
       61 agcccattag ttttttgatt ggttctttca cagtaaatag caagatacca ccgacgattg
      121 ctactgcacc gataataccg aagtaagcag attctgttcc cggtgtgtag aagcgtgcaa
      181 tttgtgcatt gattgcttgt gaagaagcat cagctaagaa ccaaatagcg attgtttgtg
      241 attcaaatgc tcgtggtgct aattttgttg tcacagacaa accaactggt gaaagacaca
      301 attcagcaat aaccacgatg aagaaacttc ccaacaacca caatggactt acttttccac
      361 ttgtaccata aagcataccc ggcaacatca atagcaagta cgataatcct gcaaataaaa
      421 gtccaagaga gaatttcact actgtagatg gttgacgttc accaagtttc gtccacatcg
      481 ttacgaatac tggtgtcaga ataaagatga aaattggatt caatgattga taccagcttg
      541 cttggatcgt tacaccaaat agcgtagatt gtgtacgttc actagcaaat aaagctaaga
      601 tagatgaacc ttgttcttgt aatgaccaga aaatgattgc tgcaaggaat aatgggatat
      661 aagcccatac tcttggtttt tcttcagcag ttacatcttt tgaagtcagc attttgataa
      721 agtaatacaa cggtaagata atccccaata cactgattgt attgacaaaa aagtcaattg
      781 tcagatgacc agttagttgt gccccgccga atactgcagc gataacaaca agtgcgatga
      841 tcaagttacg gatgaatcct ttacgttctt cagttgtcaa aggattcggc ggtgttgtac
      901 ccactccgtc taatgtcttg cgtccttgga tgatgtattg taataatcca aagaacatcc
      961 caatagcagc taaagagaac cctaaatgat agttatattc ttgaccgatt gtaccaacga
     1021 ttagtggtgc taataatgcc cctaagttga cacccatgta aaaaatcgaa aatcctgcat
     1081 ctcttcgcaa gtcagtcttt gaataaagat gtccgaccat gccagaaaca tttggtttca
     1141 aaaaggcggt tccgataata atcaatgcca tcgaaacaaa caatgtactt acaccaaatg
     1201 gtaaagccaa gacgatgtgg ccaagcataa tgaaaactcc accgaagaat acagtgcgtt
     1261 ttgctcctaa cactcggtca gatacccatc cgccgacgat actagacatg tagatcaaag
     1321 atccgtagat cgacatgata gctaatgctg ttgctcgtgg taatccaagt cccccgttgg
     1381 caactgtatc gtacatataa tagataagta atgcacgcat accgtaataa ctaaatcgtt
     1441 cccacatctc agtgaagaac aaagttgaca atcccttcgg ctgcccgaag aagcccttgt
     1501 cttgctgttt ttccataatc tataccccaa ttctttaaat tttcagaaaa tattatacat
     1561 ctttaccgaa aaatttaact aaaaaactta tttaagaaat ataaactaat tttctataaa
     1621 agcaagtaat ttttaatggc aatatcataa aaaaaagaag atagagattt aataattata
     1681 tatcagctgt ttttttcaaa taagcaaaaa ttcctaaaac acaacaatga aagcgataac
     1741 taagaaaatc cttacataac gatttttctt attaaaattg ttttttgaac ttcgcttatt
     1801 ttttgtgagc atatctgttt acttactttt tgtaaggtgc tatacttcat ttcgtaatca
     1861 attaaaaaaa tgcaaaacgc aaatcaaagg agaaatttta ttatgacaac ttttactgac
     1921 actttgaaaa accgccgttc aatctatcat ctaggacgta atgtttcact ttctaatgaa
     1981 gaattaacaa ctttgatcaa agaagcaatt aaagaaagcc caactgcttt caatgctcaa
     2041 tcaacacgcg cagttatcct tttcggagat gcacatgaaa aactttggga aatcactgaa
     2101 gaagcacttc gcccattgac tccagcagaa gctttcccaa atacacaaaa caaattagct
     2161 ggtttcaaaa atggttatgg tacagtatta ttcttcaaag atactgacgt ggtaaaaggt
     2221 ctacaagaac aatttgaatt atatgcagac aacttcccag attggtcaga acaatcaaac
     2281 ggaatcgcaa cagctaatac ttgggtagca ttagttgata aaggattggg cgcaaacttg
     2341 caacattaca acccagttat tgacgaagca gttgcaaaag aatggaatat cccttcaaac
     2401 tggaaactac gttcgcaatt agtattcggt tcaccagaaa caccggctgg ggaaaaagaa
     2461 tacatgaacg atgccgatcg cttccgcgta tttggataat tttatttgaa ataaaaaaca
     2521 gaggttgtga caaacatctt gtcgcaacct ctgtttttat tctggatcaa tcataatatc
     2581 aatctctgcc tcatcgttta agcgttggga caaaaatgga actaattctt ctaatgatgc
     2641 agaaaatttc agcatcggtc ctcgccatcc gtttcggaaa aaattcattt ttacgatttt
     2701 agaacctttt ggctgatata atacaatttt attgatctca gaaaaaggaa cacctttttt
     2761 gtcaaagcta ttcaaaacaa gtccttcttc tgtgattcct ctactgtcca acaaaaaact
     2821 aataaaaatc attgctgaca agatcccttt tgccaattca tctgctgtag gatgactgat
     2881 gagtgtatac caaatgacta gtgacactaa gaagggtatc accaagtaga cccatcgatt
     2941 tcgcacagtc cgataaataa tttcttttcg aaccagccaa agaaataaaa acatcacaac
     3001 aacgaataaa aacaatagaa ccgtaaagaa tgttaccatg acaactcgcc tcgctttttc
     3061 ttctccagaa aataggaaaa gaatactgat ttaccttctc aaagaaattg gacttgcagc
     3121 acgccgcacg tccaattgtt ttctttattc aaatgtatca tattttgcaa caaccatgct
     3181 tgctttttca tctaataatt cttggtcctc tgcagcacat tgggcaactt tgaccacggc
     3241 gcagtttgtc atttttgata caacttcacc aatgaccgtt tttgtaaaac caattggttt
     3301 gcatgcatat tgttctccta aattaatcat ttcttccgtc atcaaaatcc tcctaaactg
     3361 ctggaacttt tctccacatt accactgcta tttctttgga gatttaactc attgattttt
     3421 atttacagtt aaaaatcaac cttacaggca ttatagcgta ctgttttcgt tttcacaatt
     3481 aaaacagctg ttgtatttag aagaattttt ttcaaaaaat taaaacatat tccttaaaaa
     3541 ctaattattt cctacatatt aaaatataat acttccattt ccaataaaaa tacatttata
     3601 ataggaaaga taaacagctg aaaagggaga tttcaaatga agaaaaaaac gttcaaaaaa
     3661 ctattgtttc tagggggcac agctgctctt ttattaagcg gtacaagtta ttacctacat
     3721 atccattctc cagaacatac aatgaacact gcacaaaaaa gttcaacaca aaaaacgaaa
     3781 aagacaaaag agaaagcaga agttttacta gatgttccat tagagagcca gtttgattct
     3841 ccttctttag aaaatggctg tgaagtcact tcgctttcta tgcttttatc tttttatggt
     3901 tatgaaacaa ctaaaaacca gttagctgag caattggatt acgtccctgt tttcaatgca
     3961 gatgggactc atggcgatcc taatgaagga tttgttggtg atatcagtgg cggagactgg
     4021 gctatggtcg tctatgttcc cccagttgcc tcccttgctc agcaaatcgt ccaaacggat
     4081 tatcacactt ttccaaaaac cgatgctcaa ctcgatgata tcaaaaaagc actatctaaa
     4141 ggaaatcctg tctggacttc tgtgactatc aattttgaag tcccagatga aactgatttt
     4201 atgacttgga ccacaaataa tggagaagta agagtcactc ctcttgttca tgcttgtgta
     4261 gtcactggat atgacaaaga aaatatttat gtcaacgatc cctatggtgt aaaaaatcga
     4321 gcagtaccga tcaacgattt ttcggcgatt ttcactgcaa tgggtggaca gatgctaact
     4381 ttggagaaaa gctagtaatt caaacaaaaa tcccttatac tcaagatacc aaacctttga
     4441 agggagcgta gataatggaa aaattcacac ataaaaaaat ggatccaaat gaaattccga
     4501 ttatttttgt ccgtgactgc aagggaaatg ttcaaggtaa agtctctatt aatgagtgga
     4561 acgagcgtcg cagacctgct accctcaatg aactggaaat caaattgtat cgtcaatctc
     4621 ttgtttatta cgcagatcaa gagtacgaga aagccaccga tttattgaaa tttctaattg
     4681 ctcgaacaga gtatactcgc tttgaatata ttgaacgttt agcaaacatc tatcatatta
     4741 tgaatgaacc tgtcaaagaa tatcagttat tggacaccgt cttgtcagta gcagaactga
     4801 ttgcccttcc tgctggatta gaaaagaaac tagtacgccg ccttttacga gtaaagcaac
     4861 aactatccga tcaagaaaaa taacttgcgt ttttttagga aagtgctatg attattttgt
     4921 tgaataaatg ggatgtagcc aaactggtaa ggcacttgac tctgaatcaa gcaattgtag
     4981 gttcgaaccc tgccatccca atttgatgtg cttgctgaaa aaatgaaccc cctgtaaaag
     5041 aacaatcttt ttgcaggggg attattgtat tatttttact acttgtattt agtttacata
     5101 attaaattat gaataataaa atcgggcgta ttcttaatac aaaaactgat ttacccaata
     5161 attgctaatg ctttctccgg aactttttct ctaggaactt cctcatacgt ttctatgtgt
     5221 gctcctttat tgctaatttt caaataatga ttttctttta acacagaaag accattaaat
     5281 tcaaccgttc gttctttccc attggcatca gcagctttct gcacatacct tgctgtacca
     5341 tattcattta cttcctctgg tttttgtgtc tttacataga cctctccttt ttcaactaat
     5401 ggatttagct gatcaagtat accagggatt tctccataac ttccttcagt ataatatttc
     5461 agtccaaata gcaaaatacc actaatcgcc gccagtccaa tcaaatattt aatcactttc
     5521 atctttctac ctccttgtta tatattcagt atatgggtca gttaaagaaa ttccatccgt
     5581 cccaagttgg agatttttct cctactttag aagcgagaat cgagtgtttt tttagtaaaa
     5641 atgatcgtta tttgatatta ttgacgtgtg gataaatcag tttttctcgt tttgtccatg
     5701 taaaaaatca tgttgatagc aaaggatgtg ttcaaaatga atcaagaaga attatttcaa
     5761 aaagtcaagg aaatgattaa gaacggcaat ttcgatggcg caaaacgatt tatcgaagaa
     5821 cataaagaac aattaggacc ttataaagag aaagcccaaa atcttttaaa agatgtaaat
     5881 atcgacagtg tcaaaaacaa atttaaaaat ctattcaaat aagtaaaact ttcacttttg
     5941 taaaatcaaa ggaggacaat gctgctgcat cgtcctcctt tggttctttt ctgaatactt
     6001 catactaaaa taaacgtgtc ggatcgtaat gataagtcac attgccgttc agtgaggctt
     6061 ttcccaccac atatttacga ttctccaact tcagccacgc ttctcgaaaa gccattaatt
     6121 cttctctttt tacatcaatc ttttcgattt ttccatcgat caaatcttga ataagttgtt
     6181 catatggatt catttttttc ctccatctgc tgtcaatcaa taagttgtaa aaataggttc
     6241 atatgctttt ttggtatcta gatcttctct gactttgact gtcgtataaa taattttttc
     6301 tactgggata ctataataac ttccttcacc attattgaat cggtgaacgt ctttctttgc
     6361 taaaatacca tttattttat tcgtcagtac acgttgttcg acactgttac ttacaacata
     6421 gtaaatcacc cgaatattat tgatcaaatg caaatcgatt tctaacacta catgatccat
     6481 gaaaatccca ctccttcttt cactttgatt atagcaagtt tctcctctaa tttcaaaatc
     6541 gatacagatt ttaccggata aatccacaaa actgcttgcg ccatttcgca caaagtgtta
     6601 ttttgtatca tggactttat ttatcggagg caatagtatg atcgcgatcg gtcaatttgt
     6661 tttttatatt ccttttttta ttatgataag cattcttttc tattacataa aatggacaaa
     6721 aaagaagttt tcagttttac tggcctcttt acctgctgta tattttactt atcaaatttt
     6781 ttcttttagg cattgggaaa caacgtctgt attaataaca cacatcatcg aattgacgct
     6841 ttcagtaatt tttcttatta tttggatcta ttttttatat aagaatcaga attgaatccc
     6901 tcgcatgttt aaaaagaaaa cgattaaaga tgcagcaaat ttctgccgat ctttaatcgt
     6961 tttttctatt ctgttatacc aaaaaaatgt ttgaatcggt gaaggatcgt tccttgtttt
     7021 aattctataa attcttgtgc gatcgggtac ttttcttcca aattttcttt gttcttttcg
     7081 atcaggcttt tcgcttcttc atatttttta tctgcacata gtgcttttac ttcctgtaat
     7141 acttttcctg cttccatttg cctcaccctc ttgccatttt attgctttaa gttttattta
     7201 agttatacgc attattctag tttcatccca aataaccttt tttcattttt actcctcgaa
     7261 ctaatcagtt ttctaaaggc tgattggttc gtctttttta tttttcctcc atttcattct
     7321 gctcttctgt taatttatct aatgctttga ttgatgctgc ggctcttgca taatcatagc
     7381 tgtataattc cataaattta tcaggcagaa caaaccacgc tggatgataa ccgttagctg
     7441 tgacagggaa tcctttatct tgttggttat ctccatccca tctcagagca acaccttttt
     7501 ttcctttcca ctttactaaa gcgatcgagt atcccttctt tgtttgtgtg ccgtcttcaa
     7561 taattgctaa aatttctatt ttttcttttg gcgcacggac ttcttgagga cgaaccattt
     7621 tctattcctc ccttatctca ttacttcacg accaacacac tgcaaggggc atgtgatacg
     7681 acataagaag cggtcgatcc aactaatgct tgttgtatag caccttttcc tgtcgctccc
     7741 ataacgatca aatcaatatt ttcttgtcca gggatgacat tggcaatcaa atttcttgga
     7801 tctccagttt caactattgc gtggatctca gcaatgccgt actctttggc atcattgatt
     7861 tttttcagca tggctacttc tgttctttgt ttttcatctg catagatttt tgaaaaggca
     7921 taggcactat gggtcagctc aactttgttg atgattgaca cgataaataa gtgggcttca
     7981 ttgtcctttg ctaagtcaag ggcttcagcg aacgctttga tcgattgtct tgatccgtca
     8041 actgctacca tgatgttctg gtatctttta ctcattggaa ccgcctcctt tttcttttat
     8101 tgtaacatct caagcaaaaa tacaaaaaat atacgcaata tgtaaataaa atgatgaaca
     8161 aaagcaattt ctttcaagtt atttatacat ttcttttctc atcagaacac ctgttctaat
     8221 cccattattt ctgcattatg tgaaattcct catcattttt cacatcgagc gaacatcatt
     8281 ctgttacaaa aaacgtggat ttgtcataaa ttgtcatgct tcttttacct atgaaatatt
     8341 ttcacgtgtt aaagtattcc tcgttgaaca caacaaataa aactttttaa cgaataaagg
     8401 agatttttca tgacatcact taaaacttta ctttttggaa caactttggc tgccggcgct
     8461 gcattcttca tgggaacgac tgctcatgcg gacgaagctt ataccgttca atcaggtgat
     8521 actctatcaa cgatttctca aaaatacgtt ggtgataact ccttgatcaa tgccattgca
     8581 gaatcaaatt caatttctga tattaacctg atctactcag gacaacaatt aacaatccca
     8641 acagaaggct ctgcacaagc agctgctgaa cctcaagcag ccgttcaaga agcaccagtc
     8701 caagctgaac ctgttcaagc tgaacaacct gttgtacaag aaacggttca aacggaaaca
     8761 caagcagcgc ctgttgctga aacacaacct gctcctgctg taacagaaac tgctgctact
     8821 ccagcaagta caagctctgc aaaagaatgg atcgcacaaa aagaatcaag cggttcttac
     8881 acagcaacga acggacgtta catcggccgt taccaattag attcttctta cttaaatggt
     8941 gactactctg ctgcaaacca agaaagagta gcagaacaat atgttacttc acgttatggt
     9001 tcttgggaag cggcgaaagc attctgggaa gcaaacggtt ggtactaaga ttcattattt
     9061 gaacgtttaa acacattcaa aatatagtaa gaggtcatgc gatttattct ttcgcatgac
     9121 ctctttttga gtttaagttt agtctttatt ccttaactat cgtttgccag tatttcttgt
     9181 atcagaatct ataaatctac tatttgaatc aatctattag gttttaaact gtcccacctt
     9241 cacacaaatt ttttttgatt tatttgaatc tggttccgtt gtttaaaatt tttcctatat
     9301 tcttttagat aatatgttat tttctttctc atcgctccag gtgtaccttc tctttataaa
     9361 aaaacaaatt atgcggctaa gaagactcga acttccacga ggtcaccctc actagcgcct
     9421 gaagctagcg cgtctgccaa ttccgccata gccgcatcac atcttttagt ttaggattac
     9481 atgtgttttt tgtcaagaac atatttttgt tctttttcat ttctgtacgc cctcccttat
     9541 gccatctaca caaataattc atccgagcaa ctccatcatc tattgagggt tggtgaaaaa
     9601 atctgtttta tccatatgat cgatttcaat gttaaacgaa cgactctggg attagtaact
     9661 attttatttt tttcttcatt tcttttttcc tctttttctc gattcagcta tcttctttca
     9721 tttggatatt gattatttct tctgttgatt actatccttc tggaattcta gacgctgaag
     9781 taattcacgt tttctttctt ctagagatca taagtaacga atgaacgaac ttcaaataat
     9841 ttttttattg taattcatat ttgtattgtt cgatgattct taatacgata tgacacaata
     9901 aataaaaata ggcatggcta tttacaacaa tagtgctata gtagaaaaca aatgaataag
     9961 gttacttaaa gaggtgaata cgatgaaact gatcaaacta tgggaagcag atttaaagaa
    10021 agcttatcag ctacaaacta gctttggacc aaatgagagt ggatttatca acaatgctta
    10081 tggctataca tttgctgaat ttttagatta tgtaaaaaaa tgcgaaaaaa cgtctttagg
    10141 aatcgatctt cctgagagtt acgttcccgc aaccgtcttt attttagaaa gtgatgaaca
    10201 tgagtatgta ggaattttta atttgcgcca taaactgaac gaaacattaa gaaacggcgc
    10261 tggccatatt ggttacggca tctcccctaa ttatcgtaaa aaaggatatg ccacacaagg
    10321 attagcatta gtactacaag aggcaaaaaa aatcggcatt gatgaggcac ttttgtctgt
    10381 aaataaaaaa aatcttggca gtcttaaagc acaacaaaaa aatggggctg cgatttatcg
    10441 tgaagatgag aaagaatact atacgaaaat caaactgatg taaaaaagaa ggattccaaa
    10501 aggaaaggga tttgaatgtc ctttatcaat agaacacatt tatttaatta acttataata
    10561 tagcagacaa aattttataa acaaagaaac cttttatctc ttttcttcta gaaaggataa
    10621 aaggcttttt tgtttttcct atttgcttct catagctagt cagccaagat ttctatcgta
    10681 tccccaacgc aatccgtgca taacggctca tcttatctgt cgtccatgct ggataccaga
    10741 ctagtttgac ttctgctttc gttacttctg gcacttcttt taatgcgtca tggatagatt
    10801 ctgttaaaat atctgctaat ggacagccca tagttgttag agtcatctta atgactgttt
    10861 ctccattttc aggattgaat tctatatcat aaatcaagcc taaattgacg atgtcgattc
    10921 ctaattcagg atcaatcacc atttccaagg cagcaagaat acgttctttg attagttcta
    10981 tttcttgtcc agaccgttct tggtttgctt cgctcatcgt acgacactcc ttaacacgat
    11041 ttttatttat catactcttt ttttagctgc ttgtaaatga gaatttagaa gtaagattct
    11101 cgtttaaatt tataaaaatg ttcttattag atgtaataga ttaaatttat tctaaaataa
    11161 acctgattcc tcattttttt tgtgaacgat tttttatttc tggcaatacg ccagttgttc
    11221 gatacatttt ttgttcgttt tcatttgcaa accggccaaa tactgaatga gttggtaaaa
    11281 tattgataaa tgcatcgtcg tcgatttcat gaacgatctg atcaagatca tacatttcgt
    11341 agcgggtgat gaccatcatg atcatgttcc cttgtttttt tgaatagcca ccgacagacg
    11401 gcagtaatgt cattccacgt accatccgtt ttgctagttc ttctgttacc tcttctggct
    11461 tgacggtaac gatcattgct gttacttttt gatgactcgt gtggatggca tcaattactt
    11521 gagtcattgc ataaatggaa ataatcgtat acaaggcact ttcccagtta aagaagaagc
    11581 ctgcgatgac tacaatgata ccatttagaa tcagcatgta gtttccgact gtttttccag
    11641 ttgtttgtga aagtacaaga gagatgatat ccatcccacc agttgtaaag cccattttta
    11701 aagataatcc tacgccaaga cctaataaaa caccgcctac tagggcattc atcaaaatat
    11761 tatcagtgat ctgtccgact ggaagcagca tcgtcatcac cgatacacat acaacattca
    11821 caaaactaag gatcgttgct cgttttccta ctttgagaaa ccctaataca aacacaggga
    11881 tattcaaaag aaaaataaat gttcctgtgt ctatcttgat atgaaaccat tcatataaaa
    11941 gcgaggaaat gatttgagcg atcccattca tccctgcaga aaagacattg gctggaatca
    12001 aaaaataatt taaagcgacc gcagcggtca atccggttat caaaataacc actattttct
    12061 ttaatctttc atttttatga tacgcagtca taattttact cacaagcacc tctcccctac
    12121 tttggtattc caaatgtaag aataacagtt tatctattaa aataaaatac ttcgaactca
    12181 aatgagaaga attttagcaa acaggtaaga atctgcccta atcctatgat aagatagatg
    12241 tatggtaaat atttatatgt atatcaggag ggttcctatg gcaaaagatg ctagcttcga
    12301 cattgtatca gaagtcaata cagaagaagt aaaaaatgcg attcagcaaa caacaaaaga
    12361 attgtccaat cgctttgact tcaaggattc tacagtagaa atcaaattag agaacagcaa
    12421 tctagttgta ttgagcgatg atgatttcaa aatcgaacag atcaaagatg tattatttgg
    12481 taagctgatc aaaagaaatg tgccaatcaa aaatatccat ttttccgaat cagaacacgc
    12541 tctaggtggc aaagctcgac aaactgccga tttggtcaac ggaatcgatc gtgaaaacgc
    12601 gaagaaaatc actacagcta tcaaaaatga aaaactgaaa gtaaaagcac aaatccaaga
    12661 agatcagatt agagtgacag gtaagaaccg tgatgatcta caagctgtga ttgctttatt
    12721 acgtaaactt gacttaccaa tcgagttgca attcactaac tatcgttaat caaaaaacga
    12781 ggctgacgtc tgtcattcct cgtttttttg atttactgct tcttttgata accaaaaacg
    12841 ctgtattcct tcttccaatt ccccaacact tcgtacttct cttgctgttt gccaatgttg
    12901 tgggaataaa cgtcgataag aaaaactaga aaatcgttga tctgccaata caacgattcc
    12961 ttgatccgcc atgccacgaa tcactcgacc accagcttgc aagactttgt tcattccagg
    13021 aagctgataa gcgtaagcaa atccaaggtg ttccttctca tcataatatg atttgatcag
    13081 ttcctgctca tgattcatct gcggcaaacc tacacctacg atcatactgc cgatcaatcg
    13141 gtcccctctc aaatcgattc cttctgaaaa aatcccgcca agtacgcaaa atccaatcaa
    13201 tgtttcagcg ggatttgttt gaaattttgt taaaaatcgt tcgcgttctt cctcattcat
    13261 gtccgtttct tggattagca cattgctgtc gggatattgc tgtctgaatg cttccaccgc
    13321 aagatctaaa tattgataag aaggaaagaa aacgaaataa ttcccgacct ttccatcagc
    13381 aaaagcatga atcgtctcta ctaattttgc cagactgtgt tccctttttc gataagtcgt
    13441 ctctaggtaa tttgcgatga aaacttgctg gtttccttct gggaaaggac tcggcagttt
    13501 gtaagccaga cttttttgtc cccccagtgt ttcctgataa taagagagtg gtgaaaaact
    13561 agcagaaaaa agaatactgc tttttccttt atccaacgac tgctcaagaa acaagcttgg
    13621 atcaatacaa aactctttta ctatcagatc gcgatacgtt ttttcgactg ttgtttcgta
    13681 gtgatcatca taaaattcac tgaccttcaa aaaatgaagc aaatcaaaat aataaggaag
    13741 caattgttct tgtgatgggt gctcaggaaa ttctgccagc cattctttaa ttttttcgct
    13801 taattgataa cctgccttta ctaaagattc agccggtgct ttttgatgat gataatccca
    13861 atgatcttct tttgctattt ctcggatcga atcaaattct tttaatagtt tgttgaaacg
    13921 gcgatgaagt tttttgaatt cctttggtat agcttcctta cttcgtttcg ttttttcata
    13981 agatagttct gccgaataca tctctcggga acgattcacc aaattatgtg cttcgtcaat
    14041 caaaaacaga tagtcctcgt ttttttcatc ttcaaaaaaa cgtcttaaat aaacagttgg
    14101 atcaaataaa taattgtaat ccccaatgat tacgtcacac cagatactga catctaatga
    14161 tagttcgaaa ggacaaagtg tatgctttct tgcataagtt tcgatgactt cacgagtgat
    14221 ctgattctca tgattcaaaa gatcccataa accttcatta atccgattgt aataaccatt
    14281 ggcatatggg cactgatctg gattacaggt cgtctcatct aaaaagcaga tcttgtcttt
    14341 cgccgtcaaa gtgacacttt ttacttcagc tcctgtatct tttaatgctg tcatagcatc
    14401 ttcagctact tgtctagtta ttgttttagc cgttagataa aagatctttt ctccctcttc
    14461 ttcccctaca gctttcaaag cggggaataa cgtagagatt gtttttcctg ttccggtagg
    14521 tgcttctaca aacaatttct gtttcgtacg gatcgtttta taagcagcta ccgctagttc
    14581 acgctgacct ttcctatact catcatgggg gaatttcaaa gccatcaaag aggcattgcg
    14641 tacagtgcgc cagttttctt gaaaaacaag ccattcttta taatcttcag tcagcttttt
    14701 aaaaaaagtt tctaactgct ctcttgtctg atgttcaacc tttctcgtga tcacttcttc
    14761 tgccgtctgg aagtaagtca attgcagatt tatttcttgt aaatcatgtt cttgactata
    14821 gatataggca tacacccttg cttgatgaaa gaaaagttcc acttgttctg gttctaaatc
    14881 ttcaaatcgt ggttcggagg ttttgatttc atctatataa taaataccgt ctcttttgaa
    14941 aataccgtct gcacggcctt caacgatcag gtaatattca tctaattgaa ctgttgtttt
    15001 taaaaagact tctttttcat agttttctcc cgcatctttt tggagcttgc ggtggattct
    15061 cgctccttct aatgcagtat ggttactttt tttacgattg tcgatacttc cacgtcgcaa
    15121 aataaattct actaagtgcc gcacagatat ttttgactgt ttgatcattt ccaccacctc
    15181 tttctctcta agattacctg aaaaaaccta gtttagcaaa aagagctctc tttccaaaca
    15241 taaaaaaaca ccctgctgat taggcaggga tataggatat agaatttgga tttttggatg
    15301 aatctaatgt aacaaggaca tattaaaatg ctatgaaaac ttactttcct tctctttaga
    15361 atatccaaat acttttcata tagtctgata attcagataa gtaaagagaa ataagtacct
    15421 gtttttgttt cctagcaatg aatgcgttat actaatggat gaaagaagtt ttggctatca
    15481 aggaggaatg cacatgcata tcgaaagatt aaaaacagga atgattgaag aaaactgtta
    15541 tttggtctac aacgaagaag cacttttgat cattgaccct ggagaagatg cggaacgaat
    15601 caaacaagaa atcaaaaaaa caaaccaaca gccggtagcg attttgctta cccatactca
    15661 ctacgatcat atcggtgcag tggaaccttt acgcgcgtat tatcaaatcc cagtttatgt
    15721 cagcccacta gagcaaaaat ggctaggtga tccgattttg aatctttctg gactcggtag
    15781 acatgatgat attgctgatg ttattgtctc gcctgcagaa tatgaatttg aaatgaaaac
    15841 ttatacttta ggaaacatgt cttttcgcgt cgtccctacc ccagggcatt ctataggaag
    15901 cgtcagcttt atttttgatg attttgtcgt ttctggcgac gccctgttta gaggaagtat
    15961 cggtagaacc gatctgtata cagggaattt agaacaatta cttcatagta ttcggaccca
    16021 gctgtttgta ttacctgatg aatttgcagt ctatccagga catggcgatg caacgacgat
    16081 tgaacaggaa aaaaggacaa atcctttttt caatcgttaa aacgacttag gaggagaata
    16141 aaatgacaga aactacactt tatatcgtac gacacggaaa aacgatgttc aatacaattg
    16201 aacgagtaca aggctggtgt gatacacccc tcacaaagca aggacaagaa ggcattcatt
    16261 atttaggtcg cgggttaaaa aatgtcgact tttcatttgc ttattctagt gacagtggtc
    16321 gtgcaatcga aactgcccgt attattctag gagaacattc taagggaaaa gaaattcctt
    16381 attttattga aaaaaggatt agagaatggt gtttcgggtc tcttgaaggt ggatatgaca
    16441 tggaaatgtg gggtgtgatc cctcgtgtat tgaattttaa aacatatgat gaaatgttta
    16501 ccacagaagt ttcttttgaa caaattgcta atgctgtata caacttagat accgctgggt
    16561 gggcggaaac ttatgaaaca ttgcgagacc gtgtatggac aggatttgaa gatatcgccc
    16621 atcactgcga aaaaaatggt ggtggaaatg ttttagtcgt atctcatggt ttgacgatcg
    16681 cctttttgct taatctgatc gatcctaaac agcctgttcg tgctgggctt ttaaatggca
    16741 gcgtaacaaa ggtcacttat gcaaacggtc gattttctat ccacggcatc aatgatacgt
    16801 cttatatcga taacgggaaa acacatagtc gcgactcgtt tctataatgg ggatatttta
    16861 atttttacca gagaatcata aaaggcaaag tctgaacgag ggctttgtct tcttttttct
    16921 cttcgtgtac ttatctgctt tatttctcaa gtttttctta atattaaaaa acagattggg
    16981 tttctcaaaa aataacggta tactataaga tgtatcttaa ttgggaagga aggaatttat
    17041 gttttttgca aatattatca aagcagtgat tctcggtatt attgaaggaa taacggaatg
    17101 gctgcccatc agtagtactg gtcatttgat cctagcagat gaattcatca aactggatgc
    17161 cagttcacaa tttatgtcga tgttcaatgt cgttatccaa ttaggggcga tcttggctgt
    17221 cgtcgttctt tattttcaca agttaaaccc attttctcca agtaaatcaa cggtagaaaa
    17281 aaaagatacg tggacacttt ggtttaaagt gatcgttgct tgcataccag ccatcatcat
    17341 tggtttgcct ttggatgatt ggttagaagc aaatttccac aagttcttac cagttgccat
    17401 tgttttgatc gtttacggta ttgcctttat tattgtcgaa aaacgcaaca aaaacaaaac
    17461 acctcgttgg acttctctga atgactttac gtaccaagca gctcttcttg ttgggttatt
    17521 ccaagtcttg gcacttgttc ccggaacatc ccgttctggc gcaacgatcc taggatctat
    17581 cttgattggt gcttcgcgtt ttgtcgctac agagttttca tttttcttag ggattcctgt
    17641 catgtttggc gcaagcggct tgaagatcgt caaatattta gctgaaggga attcattcaa
    17701 aatggaagaa actgttgttc ttcttgtagg aacattcgta tcttttgtgg tttccgtctt
    17761 agctatcaaa ttcttactaa gctatttgaa gaaaaacgac tttaccgctt ttggctggta
    17821 tcgtattatc cttggtatca ttttgattgg ttattggttt attgcaatgt agaaaaaacc
    17881 agaacacttc tctt
//
LOCUS       DARBFO010000050        17433 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000050 DARBFO010000000
VERSION     DARBFO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.051"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(28..726)
                     /locus_tag="SCK51_001823"
     CDS             complement(28..726)
                     /locus_tag="SCK51_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEG1252452.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(719..2071)
                     /locus_tag="SCK51_001824"
     CDS             complement(719..2071)
                     /locus_tag="SCK51_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEG1252453.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2294..3694)
                     /locus_tag="SCK51_001825"
     CDS             complement(2294..3694)
                     /locus_tag="SCK51_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEG1252454.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3855..4592
                     /locus_tag="SCK51_001826"
     CDS             3855..4592
                     /locus_tag="SCK51_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEG1252455.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4717..5196
                     /locus_tag="SCK51_001827"
     CDS             4717..5196
                     /locus_tag="SCK51_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEG1252456.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5193..5960
                     /locus_tag="SCK51_001828"
     CDS             5193..5960
                     /locus_tag="SCK51_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252457.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6190..6759
                     /gene="lepB"
                     /locus_tag="SCK51_001829"
     CDS             6190..6759
                     /gene="lepB"
                     /locus_tag="SCK51_001829"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG1252458.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6863..7465)
                     /locus_tag="SCK51_001830"
     CDS             complement(6863..7465)
                     /locus_tag="SCK51_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1252459.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7571..8040
                     /locus_tag="SCK51_001831"
                     /pseudo
     CDS             7571..8040
                     /locus_tag="SCK51_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8146..8730
                     /locus_tag="SCK51_001832"
     CDS             8146..8730
                     /locus_tag="SCK51_001832"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEG1252460.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8733..9806
                     /gene="prfA"
                     /locus_tag="SCK51_001833"
     CDS             8733..9806
                     /gene="prfA"
                     /locus_tag="SCK51_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEG1252461.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9799..10638
                     /gene="prmC"
                     /locus_tag="SCK51_001834"
     CDS             9799..10638
                     /gene="prmC"
                     /locus_tag="SCK51_001834"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEG1252462.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10631..10810
                     /locus_tag="SCK51_001835"
     CDS             10631..10810
                     /locus_tag="SCK51_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252463.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10832..11857
                     /locus_tag="SCK51_001836"
     CDS             10832..11857
                     /locus_tag="SCK51_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEG1252464.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12032..13276
                     /locus_tag="SCK51_001837"
     CDS             12032..13276
                     /locus_tag="SCK51_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEG1252465.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13429..14376
                     /locus_tag="SCK51_001838"
     CDS             13429..14376
                     /locus_tag="SCK51_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1252466.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14510..15139
                     /gene="upp"
                     /locus_tag="SCK51_001839"
     CDS             14510..15139
                     /gene="upp"
                     /locus_tag="SCK51_001839"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEG1252467.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15278..15520)
                     /locus_tag="SCK51_001840"
     CDS             complement(15278..15520)
                     /locus_tag="SCK51_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252468.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15641..15713
                     /locus_tag="SCK51_001841"
     tRNA            15641..15713
                     /locus_tag="SCK51_001841"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15674..15676,aa:Thr,seq:cgt)
     gene            complement(15832..16470)
                     /locus_tag="SCK51_001842"
     CDS             complement(15832..16470)
                     /locus_tag="SCK51_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG1252469.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16593..17225)
                     /locus_tag="SCK51_001843"
     CDS             complement(16593..17225)
                     /locus_tag="SCK51_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEG1252470.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT         5950 a         3086 c         3450 g         4947 t
ORIGIN      
        1 tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc
       61 caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc
      121 atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc
      181 ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc
      241 accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa
      301 ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta
      361 gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc
      421 tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat
      481 caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc
      541 accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat
      601 ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc
      661 gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt
      721 agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca
      781 atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt
      841 cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt
      901 ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca
      961 tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt
     1021 aattggcatt taataaagcc actaaagaaa atggataagg agctagacca atcatatcga
     1081 ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga
     1141 tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt
     1201 tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat
     1261 tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca
     1321 cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac
     1381 aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt
     1441 gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt
     1501 cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg
     1561 gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca
     1621 ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa
     1681 ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt
     1741 ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa
     1801 ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca
     1861 aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga
     1921 cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac
     1981 ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa
     2041 ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac
     2101 gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc
     2161 ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa
     2221 aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac
     2281 ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc
     2341 gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca
     2401 tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata
     2461 aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt
     2521 ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa
     2581 cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt
     2641 cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc
     2701 cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg
     2761 ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg
     2821 aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct
     2881 tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag
     2941 ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata
     3001 gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca
     3061 tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt
     3121 caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac
     3181 gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta
     3241 attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac
     3301 gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag
     3361 aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata
     3421 tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc
     3481 cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat
     3541 ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat
     3601 aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc
     3661 gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt
     3721 ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat
     3781 acagctttcg attaaaatag aaatggctta taataaggaa aatcctagaa aaaagaagga
     3841 ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa
     3901 taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc
     3961 ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt
     4021 ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt
     4081 atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc
     4141 atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa
     4201 caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc
     4261 gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc
     4321 tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa
     4381 agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct
     4441 ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg
     4501 ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa
     4561 aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa
     4621 aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag
     4681 gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg
     4741 aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat
     4801 agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact
     4861 acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca
     4921 agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc
     4981 tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt
     5041 acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat
     5101 aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc
     5161 caaacgtata accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg
     5221 gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata
     5281 cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa
     5341 accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta
     5401 ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc
     5461 tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata
     5521 atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt
     5581 atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa
     5641 atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact
     5701 atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca
     5761 tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat
     5821 tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc
     5881 tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc
     5941 aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg
     6001 tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga
     6061 gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa
     6121 agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg
     6181 aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct
     6241 attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac
     6301 cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg
     6361 gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc
     6421 ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag
     6481 tatgatgaac cttatctgga tgagttcaaa aaagaatttt cagaagataa gttacaagga
     6541 gaatatgctt atagctcagg attccaagca caagcagaaa gttcatccac ctttacgagt
     6601 gactttgaat atactgtgcc aaaaggaaag tatcttgttc taggcgataa ccgcttgatt
     6661 tcaaaagaca gccgaatgtt tgggttagtc gacaaagata tgatccaagg aaaagttgtt
     6721 ttccgttatt ggcctctttc agaaattaaa atcatgtgat gaaaaaataa aaagaagctg
     6781 tgacatgttt tttgtcacag cttctttttc cgaaaattcc ttcttatttc ttggagttgc
     6841 tcactttttt tgcaatctca ttttaagctt tttggctgat ggataagaaa agcgaagcgt
     6901 ttttgttcat aaatagcttt tccccacgat ggaaggtatg actagtagca gagacaaagc
     6961 ccgttttttc caatcgatta ttcaattctt cttggctaaa gccattatgg actttcggat
     7021 gactaatttg ttcattcttg tcaaagtcta cgatgatcaa ttgaccgcca ggagctaaaa
     7081 tttcataaag ttttgtaaga atattctccg tatcaggaat gtgtaaaagt acaagtgata
     7141 gaaggatgag attagcttgg acagcaggaa attcgacaga agcatcagcg tggatcgttc
     7201 ggacattttt cagatctgct gcttggatct tttcttctgc cattttcagc atagtttctg
     7261 aagcatcggc aatgatcagt tctttgaatc tttccgcaag cggtaaacta acaagcccag
     7321 ttccgcctcc gtaatccagc aggacttttt ggtggctgtc gcgcggtaga taagtagtta
     7381 gttcttcgcg aataatcttg gctaatgctt gacggtcagg ggaatcataa tggttagcga
     7441 tggaatcaaa aatatttttt gtcatcaaat agctccttac gtaaaacttt gaaattttaa
     7501 cttattatag cacgtgaaaa gaaagaaagc atgctatact caattcaaaa agggagaatc
     7561 tagaaagaat atgccgatat cgtttgtaaa agatagagag gaaaaaggaa agtgtgtccg
     7621 agaaatatta ctagacttgc ctgagtggtt tgggcttcct gaatcaacgg aaaaatatat
     7681 cgaggaatca agtaaactac ctttatggtg tgaaaaaaga aaagaagaat atttaggctt
     7741 tatcactctt tcacagacaa gtgaagatac tgccgaaatc tattctattg tatgggaata
     7801 aaaaaagact atcatcacca aggattagga acaaaacttt ttaaagaagc agaaggttac
     7861 gcagcaaaac attacaaata tttgcaagtg aagactgtag acgaggggca ctactctata
     7921 tatgatcaaa ctatttgttt ctacgaatca ttaggatttt caaggttgga agtttttcca
     7981 aatttatggg atgaatggaa cccatgttta gttcttgtca aaaaattgga acaaaagtga
     8041 gcaatagatt taatactaat ctgtgcttga tgaaaattct cactgtaaaa aatgctgaaa
     8101 atcaagtata ataccatagg aacaaactgg aaggtgaaaa agagcatggc acaactattt
     8161 tttaagtatg gcgcaatgaa tagcggcaag acgatcgaga tcctaaaagt cgcacataat
     8221 tatgaagaac aaaataagcc agttgtctta atgacaagcg gtattgatac aagagatggt
     8281 ttaggcgttg tttcaagccg aatcggactg aaacgtaatg cgatgccgat atttgaagaa
     8341 acagacgtat ttgacgtaat tcagaaaatg gaaagcaaac cctattgtgt gctgatcgat
     8401 gaagcgcaat tcttaaaaaa gaaacatgtc attgctttta cgagaattgt ggacgaattg
     8461 gatattccgg ttatggcatt tggtttgaaa aatgattttc gaaacgagtt atttgaagga
     8521 tcaaaatatt tattgcttta tgcagacaaa atcgaagaaa tgaaaacaat ctgttggttc
     8581 tgccacaaaa aagcaatcat gaatttacat tatattgatg gaaaccctgt ttatgaaggc
     8641 gatcaagtgc aaattggcgg aaatgaagct tattaccccg tttgtcgaaa acattacttt
     8701 cacccatcaa tgattacaga aggagaataa gcatgtacga tcaattacaa gcaattgaag
     8761 accgttatga agagcttgga gaacttttaa gtgacccaga agtgatttct gatacaaagc
     8821 gttttatgca gctttcaaaa gaagaagcaa acacgcgcga aacagtcgaa gtttatcgtc
     8881 gttataaaga agtagtacaa ggaatcaccg atacagaaga actgcttggt gaaaaactgg
     8941 atgatgaaat ggctgaacta gcaaaagaag aattatctga attaaaaaaa gaaaaagaag
     9001 tcttagaaga aaaaatcaag atcttgcttt taccaaaaga tccaaatgac gataaaaata
     9061 tcatcatgga aatccgtgga gcagcaggcg gagacgaagc agcattattc gctggcgatc
     9121 ttttcaatat gtaccaaaag tatgctgaag cacaaggctg gaaaacagaa gtgatggaag
     9181 ctaacgttac cggaatcggt ggatataaag aagtgatcat gatgatcacc ggagacaacg
     9241 tatattcgaa attaaaatat gaaagtggtg cgcaccgcgt ccagcgtgtt ccttcaactg
     9301 aatcgcaagg gcgtatccat acttccaccg caacagttgt cgtgatgcca gaagctgaag
     9361 aagtagagat cgatattgaa gacaaagata tccgtacaga tatctatcat gcttcaggtg
     9421 ctggcggaca gcacgtcaat aaaacagcat ctgctgtacg tttgactcac ttaccaacag
     9481 ggatcgttgt tgctatgcaa gatgaacgtt ctcaaatcaa gaaccgtgaa aaagcaatga
     9541 aaatcttgcg tgctcgtgtg tacgataaga tccagcaaga agcacaaagc gagtatgatg
     9601 ccaatcgaaa atcagcagtc ggaactgggg atcgttcaga acggatccgg acgtataatt
     9661 tcccgcaaaa tcgtgtgact gatcatcgta tcggattaac tatccaaaaa ttagatcaga
     9721 ttttagcagg gaaattagat gagatcgttg atgcattgat catgtatgat caaacatcaa
     9781 aactggaagc tatgcaaaat gggtaaaatg acctatcgag aagtcctgtc acgggcttct
     9841 tcttttttag aggatcatgg gaaagaagca tatagtatcc aatttttatt tttggaacga
     9901 aaacaatgga aaaaacttga ttggctcctt catatgaacg aagagatttc agaagaagaa
     9961 cagcggttga tcgaaaccga tctgcgactt ctgcttgcag atcatccacc tcagtatttg
    10021 ctaggatatg cagattttta tgatcatcgg ttaaaggtaa ctgaagcaac actgatccct
    10081 cgaccagaaa cagaggagtt agtcgaatgg tgtttagacg agacgcctgg tgtaccgtta
    10141 gaagtgatag atattgggac agggactgga gcaattgcta ttagtttgaa agcagcaaga
    10201 aaaaattggc atatatctgc tgtggatctt tcagaagaag cattagaagt agcgaaagaa
    10261 aatgctcaga aagaaggaac gaaaatcagt ttttatcatg gagatacatt aggaccagta
    10321 atggatcagc aatttgatgt gattatttct aatccgccgt acatcagtcg gaatgaatgg
    10381 gaactgatgg atgaaagtgt ccggtcattc gaacctaaaa tggccctttt tgctgagaac
    10441 gatggattag ctatttatga aaaaatagca gttgaagcac caagcgtact aaaatcaaat
    10501 ggaaagattt ttttagaaat cggatttcgc caaggtgaag cagtaaaaaa aatatttcag
    10561 caagcatttc cagataaaaa agtggcgata aaaaaggatc tttttggcaa tgaacgaatg
    10621 atccaagttt atggttgaag ttgtggatga aaaatttttt atttgttttt ttattataaa
    10681 aaaacactta tttgacaagg atgtaaacga ctttttggtt tatcaacaag tttatccact
    10741 attgacaaat agattatcca caaacagtgg aaaacttgag gataaactca gacaaaataa
    10801 gaatgtatga aaaaggggtt gtggataaac tgtggaaacg aaacgatatg atgaaacaga
    10861 attgcaagag gcagccgaag cactgaaaga aggagaactt gttgcttttc ctaccgaaac
    10921 agtatacgga ttgggcgcca acgctctttt accaaacgct gtcaagaaag tgttttctgt
    10981 aaaagggaga ccgcaagata atccattaat cgtccatgtc gcctcttttg aacaagtaaa
    11041 agagtatgtg gacaactttc atccggcagc agagaagatt gtgaagaact tctggcctgg
    11101 accattaact ttgatattta agatcaaaaa agacactttg ccctcagttg tgacgggagg
    11161 attatccaca gctgcatttc gtataccgga taacaaaaaa acactagaag taatcgaact
    11221 ttccggtgtt ccgcttgtcg gtccaagtgc aaatacgtcg ggaaaaccaa gtccaacgac
    11281 tgcagatcat gtatatcatg atctacaagg aaaaattacc gggattattg atgatggagc
    11341 tacgcgaatt ggtgtagaat ccactgttct tgatttaagt gatccaacag ccatgcctat
    11401 gattttacga ccaggtgcag taacgaaaga acaaatcgaa gcagtcatcg aatctcctgt
    11461 tgctattgat cagcatcttg taaaagaaaa tgaaacacca aaagcccctg ggatgaaata
    11521 caaacactac tcacccgata cacgtgttct gatggtcaga gatggcgatt ggtcaactgc
    11581 agttcaatgg gcaaaaaata agaagatccg tgcaggtgtc atagcaagcc ccgaaattgc
    11641 ggatcaagtg cggacagata ctgcagctgt ttatatgtat aacgacaact cagtagaagc
    11701 ggcggctaaa ggattgtttg caggtctaag aggattagat gaaccaacac ttggattaga
    11761 tttgattttt gtacaagtgt atccagaaac tggattgggg aatgcttaca tgaatcgctt
    11821 gaaaaaagca gcaggacaaa attattttga aaaatgatca attggttatt gtcgcattct
    11881 tttagtatga tacaatgcta tcaagtaaat cttctatcag tgaacgaaaa actaagcgta
    11941 actaatcgta atagaagaga tacgaatatt tggcaatttg tttttgatgg gaaaaataaa
    12001 attgtaggaa aataaaaagg agctgttgtt tgtggtagat tacaaaacgt ttgaccctga
    12061 tctttgggct gcgatcgcca aagaagaaga aagacaagaa cataatttgg agctgattgc
    12121 ctctgaaaac tttgtctccg aagcagtgat ggctgcacaa ggaagtatct tgacaaataa
    12181 atacgcagaa ggttatccag ggcatcgcta ttatggtggg tgtgaatttg tcgatatcgt
    12241 tgaaaattta gcgattgacc gtgcaaaaga attattcgga gcgaaatttg cgaacgtcca
    12301 gcctcactcc ggctctcaag ccaacacggc tgcttatctc gctttggtag aaccagggga
    12361 cacgatctta ggaatggatc tttccgcagg cggacatcta acacatggat caccagtcaa
    12421 cttcagtggc aaaacctatc actttgttgc ttacggtgtt gatccgacaa cagaagttat
    12481 tgattataat gtggtacgta ttttagctag aaaacatcaa ccaaaattga tcgtagcagg
    12541 tgccagtgct tacggacgaa cgatcgactt tgcgaaattc agagaaatcg cagatgaagt
    12601 aggcgcaaaa ctgatggtag atatggcaca tatcgcagga ttagtagcag caggacttca
    12661 tcctaatcca gttccttatg cagatatcac aacgactacc actcataaaa cattgcgtgg
    12721 ccctcgaggc ggcatgattt tgacaaacga tgaagcatta gcaaaaaaaa taaatagtgc
    12781 ggtatttcct ggtattcaag gcggccctct agagcatgtg attgcgggta aagcagtagc
    12841 attcaaagaa gcattagatc ctgcatttaa agaatatagt gaacaaatca tcgccaatgc
    12901 aaaagcaatg gtcaaagttt tcaatcaagc aattggaacc agagtcattt caggggcgac
    12961 agataatcat ctgatgttga tcgatgttcg cgagttaggt ataaatggaa aagaagcaga
    13021 gagcattttg gacagtgtga atatcactgt aaataaaaac tcgattcctt ttgaaacact
    13081 aagtccattc aaaacaagcg gtattcgaat cggcacgcca gcaatcacta ctcgcgggtt
    13141 caaagaagaa gatgctgtaa aagtggctga actagttgtc aaagcattac aagcaaaaga
    13201 tgataacgct caactagacg aagtcaaaac aggtgtacgc gaattaactg aaaaatttcc
    13261 gttgcataaa aaataaaaat ttaaattcca ttagtaaaca gaaaagttca atgttgggct
    13321 tttctgttta ttttttggga aaacggattc tttgacaata tcggtgatgc tctctagtat
    13381 atgaggtgta attttagtct tcacactaaa tctcagggag gaaaagtcat gactgatcgt
    13441 atctcgttag aaaaggaagc agtagcattc agtgatgcaa atgcgcctca tccgcgtatc
    13501 tatgaactag caccggaaga aggaagagtt ttactagaag aagtacaaga atcacctgtg
    13561 aacaaatatt ccgtcgatat tgaagacttg atggtggata ctggcgaatg gggaaaactc
    13621 aatattcgtc tcgtacgacc agaagggaat atggataaat taccagttat tttttatatt
    13681 catggagcag gctgggtttt tggcagtgct caaacccatg ataaattgat cagagaactt
    13741 gcggttcgta cgaattccgt cgttgtcttt ccagaatata gtcgatcacc agaagcaaaa
    13801 tatccaacag caattgaaca aaattatgct gtgttgcaac agctgaataa attagccaag
    13861 tcaaaaaatc tggatctcga gaatctaatc gttgcaggag attcagtagg tggtaacatg
    13921 gctacagtca tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc caagcaattg
    13981 ctgtattatc cagtcacaaa cgctgatttt gatacagatt cctatcatga attcggagaa
    14041 aactattttt tgacaagaga aggaatgatg tggttttggg accaatatac gacgaatgaa
    14101 gaagagcgtg ctgaaatcac agcttcgcct ttgagagcaa atataaatga actaaatgat
    14161 cttccttcag caatgatttt aacgggagaa gcggatgtat taagagatga aggtgaggct
    14221 tatgcgcgca aacttcgtga tgcaggggtt cctgttacac aagtacgctt ccaaggaatc
    14281 atccatgatt ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg tgcagcaatg
    14341 gcattatcta cttcttggat caagaaaaaa gaataagctt tgcttgtccg caggaaaact
    14401 tcttttcaaa aacggatttt cttaggaaaa atggcgagtt tgactagtca atcacttgat
    14461 tttgttttac aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa tgggcaaatt
    14521 tcaagtgatc gatcatccat tgattcaaca caaactaact attattcggg acaaaaactg
    14581 cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg catatgaagt
    14641 atcacgggat atgccattgg aagacatcgt gatcgaaaca ccgatcactg aaacaacaca
    14701 aaaaacgttg tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag gtattggaat
    14761 ggttgacggt atcttggaat tgattccagc tgctaaagtg ggacatatcg gtctttatcg
    14821 agatgaagaa acattggaac cacatgaata tttcgtcaaa cttccagaag acattgatgc
    14881 acgtcagttg tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa tcatggcaat
    14941 cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg tagcagcacc
    15001 tgagggagta aaagctttgc aagaagcgca tcctgatatt gacatctata cagcttctct
    15061 agatgaaaaa ttgaatgaac atggttatat cgttcctgga ctaggggatg cgggtgaccg
    15121 tttgtttggt acaaaataag taaatacgaa aaagggtgtg acacaacttt tgtcacatcc
    15181 ttttttggtc atttttaacg tttgatgcag aacagctttt tcacagcttt cgtttgcata
    15241 taaaaaaacc atcccaaaaa tgcagagatg gtcaaattca aatctttttt agttctttgt
    15301 agttaacgat tactacaccc ttactttgcg tcatcaagtt cgcattttct gcagtttcat
    15361 caatctcaac aacagcggaa tttttcaatt gctttgaaat cgttccagtt tgcgacttgc
    15421 cgtgaattgt gaacattacg tgagtattga tcttgaattt ttcaccaatt tccgatggta
    15481 cgatagttgt atcaaatttt ccaaaagttg ccattttcat aaaacctgcc tttcacatca
    15541 attataccac gttttatgaa aaatatcatg aaattaaact aattttccat cggactcttt
    15601 acaatcagcc aaatagagta tataatttct tttggataag gccagcttag ctcagttggt
    15661 agagcaacgc actcgtaacg cgtaggtcgt aggttcgatc cctatagctg gcattaaaag
    15721 agaagacaga tatatcaagg atttttaatc cattgataca tctgtctttt tgtttaaagg
    15781 aaataaagta tgctttctta gcaatagagc atgtattatt tctataaaaa tttaggagaa
    15841 taaaacgact agcaatatag ctgtgaaatc aaatatcaca tgaactgccc aacttgtaat
    15901 gatcgagttg cttttaatgg cagcttggtt gaataacaat cttgccaccc cttgtatcaa
    15961 aaggatgtgt gctacagtgt gtaaaatatt tccattatca taagtactgt aatggataaa
    16021 tccaaagatg attgctgaca agaaactagc gacaggaatg ctgaacttac tagaaaaaat
    16081 agcgagaaaa tacactgaga acaattcttc tccaagtatc atgataggca aaataataaa
    16141 taataacggg ctgaaagcct catttattgg attgcctgtt aaaccccatt tcaaaatttg
    16201 ttgcaaaaat accgagactg cggcactgat tactacattg ataaaaaaat atttggctat
    16261 atttttgaca ggagcgacag gtttcgagaa catttttttc gtatcttttt taccaaaaac
    16321 aagtgtaaat gctataaatc cagtgatagt taaagaaaaa atgctaataa tggatggcgc
    16381 aaatagcaac cctaacaaac cgagtacaac gacaatagtg cccaatatcc cttttttaag
    16441 ttctattttt tttcctaaaa tgttattcat gataataact cctactattc agtttttgaa
    16501 gatagagtca gtatacatga agagttaaaa taggcaatgt ttttttatgg atatttgtat
    16561 tgctaaaaat ttatcttata tattcatttt gattatacta cttttatttg taaaaatttt
    16621 ctagcaaaac caagtgcttg caactgatta agcgtacaat tttttagatg ttccgagcgt
    16681 acttcaatca tttcaaagga gttgctactt aaatcgatat tttttaaatc agtatcaaaa
    16741 aagctagcat tttctaaatc attagtagaa aacattattt tagtgaggga acagtctaca
    16801 aaatctgcgc ttgataagac agagtgctca aaggtacttt cttttatgtt tgaaccagat
    16861 aaatttacat agtccatttt acaattctcg aataacgtgt tcccaatata actttccatc
    16921 aatttagtac ctgttaattt acaattgaca aatttcacgc gatgcaacat gctttttgtc
    16981 aatatacagc ctgaaaaatc acagttttcg aatatacagt cggtaatctc cattctttga
    17041 aaatcattgg aagcaaaaaa tgcatacttg aaaatagatt tgtaaataat tggatggcag
    17101 attatttggt tttcgatgac ctctttttgt gaaaggatat tatacagttc aaaatcttcg
    17161 ttttcataga tatgaaaaaa ggatccttta ttcaagcttg cttcattaac tttaggaaaa
    17221 atcatattta attacctcca ttttcataaa gtcatattaa caaaagctaa accaacttac
    17281 attagcagaa aatgagatag gttttcgcct ttttcgataa cgatagtgtc gctatctctt
    17341 gaaacgttct tagaattgcc ctgagagaca caaattaggc gtaattctaa taaaaatctt
    17401 agaataaaaa tttttactga tttaatttag aat
//
LOCUS       DARBFO010000051        16563 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000051 DARBFO010000000
VERSION     DARBFO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.052"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..257)
                     /locus_tag="SCK51_001844"
     CDS             complement(<1..257)
                     /locus_tag="SCK51_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252471.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAF"
     gene            complement(273..923)
                     /locus_tag="SCK51_001845"
     CDS             complement(273..923)
                     /locus_tag="SCK51_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252472.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(934..2106)
                     /locus_tag="SCK51_001846"
     CDS             complement(934..2106)
                     /locus_tag="SCK51_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEG1252473.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2125..2997)
                     /locus_tag="SCK51_001847"
     CDS             complement(2125..2997)
                     /locus_tag="SCK51_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1252474.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3019..3939)
                     /locus_tag="SCK51_001848"
     CDS             complement(3019..3939)
                     /locus_tag="SCK51_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252475.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3939..4208)
                     /locus_tag="SCK51_001849"
     CDS             complement(3939..4208)
                     /locus_tag="SCK51_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252476.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4205..4585)
                     /locus_tag="SCK51_001850"
     CDS             complement(4205..4585)
                     /locus_tag="SCK51_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEG1252477.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5052..6515)
                     /locus_tag="SCK51_001851"
     CDS             complement(5052..6515)
                     /locus_tag="SCK51_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEG1252478.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6579..9596)
                     /locus_tag="SCK51_001852"
     CDS             complement(6579..9596)
                     /locus_tag="SCK51_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1252479.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9747..10586)
                     /locus_tag="SCK51_001853"
     CDS             complement(9747..10586)
                     /locus_tag="SCK51_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1252480.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10731..10976
                     /locus_tag="SCK51_001854"
     CDS             10731..10976
                     /locus_tag="SCK51_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252481.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11039..11461
                     /locus_tag="SCK51_001855"
     CDS             11039..11461
                     /locus_tag="SCK51_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252482.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11555..11872)
                     /locus_tag="SCK51_001856"
     CDS             complement(11555..11872)
                     /locus_tag="SCK51_001856"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252483.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11899..12105)
                     /locus_tag="SCK51_001857"
     CDS             complement(11899..12105)
                     /locus_tag="SCK51_001857"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252484.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12086..12268)
                     /locus_tag="SCK51_001858"
     CDS             complement(12086..12268)
                     /locus_tag="SCK51_001858"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252485.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12282..12632)
                     /locus_tag="SCK51_001859"
     CDS             complement(12282..12632)
                     /locus_tag="SCK51_001859"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252486.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12658..12960)
                     /locus_tag="SCK51_001860"
     CDS             complement(12658..12960)
                     /locus_tag="SCK51_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252487.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12963..13196)
                     /locus_tag="SCK51_001861"
     CDS             complement(12963..13196)
                     /locus_tag="SCK51_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252488.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14182..14504)
                     /locus_tag="SCK51_001862"
                     /pseudo
     CDS             complement(14182..14504)
                     /locus_tag="SCK51_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14627..15742)
                     /locus_tag="SCK51_001863"
     CDS             complement(14627..15742)
                     /locus_tag="SCK51_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG1252489.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15748..16146)
                     /locus_tag="SCK51_001864"
     CDS             complement(15748..16146)
                     /locus_tag="SCK51_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG1252490.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         4400 a         3451 c         2578 g         6134 t
ORIGIN      
        1 aaaaaggcaa gtgcttcttt gtaggcattt tgactgtatt tttttgataa agcatgacaa
       61 atttcttgat aaaatagttg actgcctacc gaataccgat tattgtgtat ccgccacaaa
      121 acgaacacac tgatttgaat cgacgggcgt aaaaaagaaa aattctttcc ataagaaaaa
      181 gaacgtcctt cttgggtttc ttttgcttgt tctgttggta cttcttgtac ctctcttatt
      241 gattcttgtc tctccataac cttctccttt tcttattctt ctaaaagctg ttgattaaag
      301 ctacttccca aattttgcat aaacgtgatt ttctttgacg ctggatcaaa ggttacgtca
      361 aaaatattca tggcattatt ccaggaacta tcaccaattt tattgcgata cgacaccaca
      421 acgagccctt ggatagtcgc tttttgtgtg ctttttaaat aaacagaaca tttctctaat
      481 tcagaagaca ccccgtttga atcaccatag gaaacgcctt ctttgaaata ttgcttcatc
      541 agtttgtcag aaagatatgg tgcacaattg ttttttcgtt cttgaaaaga atcaggctca
      601 taattaaaca gaacctcgaa cgcttctgtc actgtttttt gaaaggtttg aatgtttcct
      661 ttttctgttg aatcattgtt tttcaattct tcatttgctt tttttaacgt cgtgacctcg
      721 ctttctactt gttgaagttg ttctttgagt gacgttacaa ggtcttcctg tcctttcttc
      781 tcttcttgga acttgtttat ctccaccgtc agtttctctt cctttgctgt tagctgtagc
      841 ttttcatgaa taagaaaaaa tgtactgcct acaccaaata agaacaatcc gcctacacaa
      901 acataaataa atcgtttctt catttgacac ctcttatttc tttatcggtc gagcaaaaca
      961 aatatcacca ccattaagca aatacccaaa ggataaattg acttgccctt cacctacacc
     1021 gttagtattc attccccctt gttcgattac tttcgtagaa agtccatgcc aatcttccgc
     1081 taaaacagct gtgtgtccat catttcccac accagaacct aagttcacaa tgataatatc
     1141 gccagctttt gcctcgctct caggtatttc ttgtaagtat tgcttacttc ctcttgcgtc
     1201 gctcgtcatt gagccggtaa accacccaac gttcgcaggc gttttatacc ctgctttcgt
     1261 taaaacaagc cacacaaagc tcgaacaatc ggcatagcca tttttatctg gattttcgac
     1321 actaccaaat tgtgcccgtt gcggttgtga ataatgaaaa taaccaatca gactttttgc
     1381 tacgtttaat acattactat cggaagaact gcttgttcct gttgtttcta gacaataatc
     1441 ggtcaagtct ttacttcctg cgtttgcccc attttctaat gtaattgaag cgacgggatc
     1501 actgtcattc tttcctagtt ttgcgtacca ttgattcgca aaagtaatac gattccccat
     1561 cattggtatc cctgcacgct caaatttcac ttgccactgt tctgttgctt cctctacgct
     1621 ttttgcttgt aatgcaactt gtgaagcttt ttctgttgtg tttagttcat gaatcaaaaa
     1681 ctcaacttgt acacctaaat ctgaccactc tttgtttcgc tctttggcaa aatttttcaa
     1741 agcagtcgtt cgccctgccg tccattgtgc aatcccatgc ccagataatg gatcgtttgg
     1801 tcgttcaatc gctgtgggac tcatactcga ctcttgttga aaattgccta acatacccgc
     1861 tagaccctct aatgtcgtat ttggaatatg tttttgtact tcctttgcaa tttgaagccc
     1921 gtttgtctcc atgccttggt tcgttaatga gatagtactt gcgacaattt cggttggact
     1981 acagtcgatt tcttcctctt cagaaccact actaatcaaa agtatcataa acagaaaacc
     2041 aaacatacat agtaatagtg tgggtagaca accgaaccca caccataaaa gaagtttttt
     2101 cttcatgttt gatccttcat tcgcttagtt cattttttgt gttgcttttc ttttgcgcca
     2161 aaaagcaaaa ccgcttcctg aagccaataa tccaccaagt gctgtcgcaa cgagagacac
     2221 ttcttcgcca gtcttaggca atactttcat ttttccatca gaccctttca ccgcaatatt
     2281 tccgcttgga agttgttcga ccttgtctga aatgggcgtc aagccatttc ctgattcttt
     2341 ataagcgaca ccatcaacga cggagacaat tttttctcct gaagctgttt caattggtgt
     2401 acttggtgtt ggaatatggg ttaattcaga cgcattattg gtttcaattt gaacattttg
     2461 tccacctgtt tgttggctgc caatacttcc atctggtttt actacgacgt cctgtactgg
     2521 aggcgtagtg ttcgtccccc cattattggt aggtggtgtc gttggaggag tcgtatttcc
     2581 ttcattgcct cctggattcg acggcttttc tggtgtctct gtattaccac catttcctgg
     2641 gttttcctca acgggtggaa cttcaggtgt tccttcccct ccgccagtat ctggattttc
     2701 tggtacttca ggcgtttctg tgcctccacc tgtatctgga ttttcttccg ttggtggaat
     2761 ctctggggtt tcaggagttt ctggtatttc gggaatttct ggggtttctt ctcctccatc
     2821 tgttggtggt aaaacaggtt cttcaggaac ttcaggtgtt tcttccgttg gtggaataat
     2881 ttctgtcggt ggtacacttg gatcacctgg tgacgtagta tctgcatacg cactggttcc
     2941 taccatacct agcaatacag ttgcactaaa taaacttcct aaaatcattt ttttcatgtg
     3001 tttttcctct tctcttattt attgatttat ttcaaaatca tctagttcgg gaagcgtgga
     3061 aactagataa tttttttcct gttcttccaa ttgaaaagac actttttttg tttcttgtgt
     3121 ctttttctcg gtttccaatg taatgacata cgcaatttgc caatgcttgc cttctttctt
     3181 catttcccag gataaaatac ttttgacttt ttccttttga ggactgattt gtttcgcaac
     3241 tttttccgtt agaaacgatt gaagcttttt cctataccgt tcttctgcca tttcacccga
     3301 ataataattc gtaataaaat aacgactaaa tgtatcaatt tgtggttgtt ttgtcgcaag
     3361 ttcttccgca aatgcaatgc gttcttgtaa ctggtgctcc tttttcgtta gttgttcaat
     3421 ctctctttgc gttgaaagtt gcataataaa aagaccacaa gatacaacga cgataaatcc
     3481 aaacaaaagc cattgaaaag acattttttt cttctgaaga gagccgttgc tttgtgtaaa
     3541 cgctgtttct acagaaagtg gtgttttctt ttcctttttg atttttgttg gtcgacgttc
     3601 gttcttttgg tctttcttta cttgataaaa cttcggtaat tcggaattta aggtttgaat
     3661 aaaggtttgt gcttcttcac ttaattgccc attttctgtt aaagattgtt caatcaatga
     3721 aatcaaatga aactcttcta ctggaaaatc atacgagcct tcaaatactg gaatttcatg
     3781 gttttctgtg tagatttcca aggtacacca gccaatttcc gctcgtgcca atagctcttc
     3841 attttctctt ttcaattgaa taaatgcctt atctaatgca atttttttat ctgttagtaa
     3901 agacatgtta tcaacttctg taaatcgaat catcatcatt agtcaaacac ttcttccacc
     3961 acttctaccg catacaccac ttgaataggg ttatttaaag aaaatttcac ccgatattct
     4021 tgattgttca aactgacgat atagctatcc tcatcttcag cataataggt atattcgttg
     4081 cctttgactt gaaaatattt ttggatcgct tgtctttgtt gccattcgcc aatctttgga
     4141 aaataggcga aatgatagcc aaataaaacg cctatcccta ataaaacaat tcctgttact
     4201 ataatcatct ggtttcctcc tgtacctgtt tatcttctat tgactctttt gtcggtaagc
     4261 aataaagaac cagtaacaca agagagccaa tcgttgggac aaaatagaaa aaaataaatg
     4321 cccaatgaaa acctgcgtct cgtaaacgtc gaacgctgag agaaagagac gggacaagaa
     4381 ctacgaaaca atacacaatc aatacgccta tcaaacaacc aattattgtt tcagaagcat
     4441 tcaacgctcc tgctagtcca atcgctaagc ctaggccaaa cccaatgagg gcattcatta
     4501 acgcaacgag ccaatactct tttcttgtgg ctttcccttt gtaattaaag ctattctgcc
     4561 aaaatttttt ataatttgtg atcatatctt tgtcctttct aatgtttatt tttgattcta
     4621 gtttcttctg ttttactaac aatgaaactt gccataaaaa aggtggccaa tacaccacca
     4681 gctagaaatc cacttacaaa taacaaaatt ttttcctcct ttctaaaaaa tttcgccata
     4741 aaaaggcaaa gtatgaacac gataaatacc atgtacatac tttgcctttt tatgcaatat
     4801 atgttaactc tccaagcaat tactgatttt taaattcttt ataaataaaa taaaaatcgt
     4861 ttgagtaaag aaataagtca atgaagaccc aaacgataaa aaatattcct aatactatat
     4921 ttaataaact acgttctaga tagatacgag atttcaaatt aaaaaacgaa aatgagagat
     4981 acaaatatat caaatattta aaaagtaacc acattttcta taccactcct tatcagaaac
     5041 agccttattc tttagaataa gactgtttct gggttgatta atttgtcttt tgttaaacta
     5101 ccatctaagg caagttctaa atgtaaatgt ggtcctgtac tatttcctgt tgaacccaca
     5161 taaccaataa tttgtccttg ttcaactgtt tgtcctacgt tcacgcaata ttcctgttgg
     5221 tgcgcataaa gagccacact tccgtcttca tgctcaattg tgacataatt tcctgacaaa
     5281 gtatgatacg aagcttctat gaccgttcct gccttgctcg catagattgg ttctccttga
     5341 ttggctgcca tgtctaatcc tctatgaaat tcttctcctc gccaaccgaa ggaactagaa
     5401 attgaataat tttttagagg taccatatag ccttctttgc ttagttgtgg accagctact
     5461 tctttatcat atttgattaa gtcataggtt tcgattaacc catttaattt tttatcgtag
     5521 ctagtgtctg ttgcgtatct tcctgttaaa aatttagtcg cttcctgata ggtttttgcc
     5581 ttacttttcc aaacaccatc ataaaaagag gtatttccaa ctaagccttc ttttaacagt
     5641 ttggcatagt cttttaagct ttcttcatag ctctcatatt gccgaaaagt tgcttgggtc
     5701 gtataaagtg tcccattgcc taaatcttct tgtgtcgcaa aagagacgcc ctttccgtta
     5761 tgtgtgcctt tgattccaaa tagattgtag tttggtgcct gagccaattg actttgtccg
     5821 cttgcggatt ctaaaatggc ttgcgcgatc ataacagaag cgtataaatc attttcttgt
     5881 ccaattttac gagcagattc accgatttta cggataaacg tttctacaga cgcgtctttt
     5941 tcaaaatgaa tagtcccttc gttatcttgt gtgctggaag ggactaattc ttgtatcggt
     6001 gcgcttggtg cttggtggta gttttttgtc tcttctgaac cgattgattg tgcctgcggt
     6061 acgtcattca taggtacagt cacactctgt tcaggtgcct tttgtgggcg ttccgttttt
     6121 gttggcggtt ccgcttctgt ttcggtactt ggtttttgag gagtctgtga gctttcatca
     6181 gaaggttgct catttggttc ttctgacggt tcctcctcag gttgttgatt gtcttctggt
     6241 ttgtctggtt gattcggtgt ttctggttgt tcttcagaac ctgttggtgg agcgttcatt
     6301 tctggattat ctggtgtatc tattgtatct tcagtcggtg tttctggttg attttcttct
     6361 ccagtggaat ttgttgtcgt ttcttcagtg atcgttgcag attgcgaatt tgtcagttga
     6421 ttgttgcctt gttcattagc caaaaccgct ggcgtatacc caaatacttg acctactaat
     6481 aacaaagcag ataaacattt actcgttttt ttcacaagat tgcctccatt cttcaatcaa
     6541 tcatctagag acagctgatt gctgtctcta gtcatacttt aattgctttg tttcatttta
     6601 cgacgaacca gaacagcccc cacaccaaca aaaagaaaca gtcccaatgc aattaacaca
     6661 tcgttcactg tttctcctgt ttttggtaag ctttgttttg gtgtgctcgt cggcgtttgt
     6721 ttgacagggg ttgtcggcgt ttctttttta atgccttttg ctgtcaatgt ttgtttttca
     6781 ttcttcaaat caaagtgacg tgcttcttcc tctttatggt ctttatcctt atagacaata
     6841 tgagttgcga ccaatttctc cccgtctttt aacgtgcctg ctttgtagtc aaaggatact
     6901 ttaaactctt cagacgcttt agaaacttta cgtgcagttt ctaccgtacc aacgactgta
     6961 tcttttccat ttttatcaat cttatggaat tgagtcaccc agtatttggt ttcccctacc
     7021 gtagattctg gaaattcttg cgatacatga tcgatcaact cttggtcttt gtctggatca
     7081 aatgtttggt cgccattcac tgtaataaag cgtgtttcga tttttggttc tttcgcagtc
     7141 actgtttgat ctttattggt taaatcaaaa tgacgagcga attcttcttc ctcttctttg
     7201 tcgttataga caatgtgcgt tgcgacaatc gtgtcaccgt ctgaaaccat gtctgctttg
     7261 tagttgaacg tcacatcaaa tgtttctttt tcatcggtca cttttcgtgt ttcttttacc
     7321 gtatctaata cagtgacttt tccatcggca gtaattttat ggaattgcgt cacccaatat
     7381 ttctctgtcc caaccgcaga tttagggaat acttgggaca ctttatccac taattcttgg
     7441 tcttttttcg ggttgaaatc ttttccacct gtcgttgtca caaacaaagt agaaatttct
     7501 ggttggtctt cttctggaac cacgatttct tcgtttttca cagtaattgg ttgtacttct
     7561 ccgtctttga ccgttactaa aatcgtttct gtttgtcctg acgcttgccc ataatgctca
     7621 gggaacacaa tttccgtaaa tttatacgta ccgtctggta attttagttt ttctggttct
     7681 tcttttgttt catacacaaa ggacacattt ttattatgtg cagtcatacc ttcaatcagg
     7741 aatttcgctc ctggtaattg tagatgattg attgaatcga ctttattgaa gtctacttca
     7801 cccgttttta acaagttgtc aatcacatct aattcaaacg taagcacagg cgtcgtgtta
     7861 tcgccatagg ccacagagaa cgtggtttgt tcttcagaag gttgacggta gttgtctggc
     7921 acattttttt caatcaattg gtatttgttt tgatcgccat tgtagaattt tttgtttgcc
     7981 ttccagtgtc cgtctttgtc agtttttact gaatccaaga cttctccttt tttcacaagg
     8041 actgtttctt tatctggcgc agtaatatct gttttggcga taatatcaaa ttcgatattt
     8101 tctgcaggaa cggtatcata aacaggtttg ccttgattcc aggattcttc cgcattatag
     8161 acttctttga ctttctttcc ttctggaatg aactgttgta agtcattttt tgcagttaac
     8221 gtcacttctt tgtttggttc aatcgtaatc gtatgagttt gtttgtctag aatataccca
     8281 gctggtgctt tgacttcttt gacatccaat ttattttgaa catattttcc gtctttttca
     8341 tagaatgcag ggatttcttt taaggcgatt tcccctttgg catccgttgt atattgtttt
     8401 gtttctcccg tatcatgacg tttgatttca tacgtcacat ttggaacttt tttccctgtg
     8461 acagcgtcta gtttggttag tttgaagtcg ccatttttta gaactttaat agttagtcca
     8521 gctctattag gatctggcac ataaagcttt cctagatttt gagcaacagg atctacatag
     8581 gcaaaacttg aacctacccc tctagaattt ggatccagtg aagaaacaga aattcgtccg
     8641 tcatttgagt tttgatttgc ttttataaac aaactatttc ctgattttct tacttgaacg
     8701 ttagctgtgt tttcaacaac cgacccatcc cattgagaaa acacaccttt tgtatcctta
     8761 acttctactt cttcccctac tttaaccgtc actgtttggc tatcaaaaga aggatcttca
     8821 agtaaattgt ttatttttct atctacagtc tgtttgaaag cttcataatt agattgtcca
     8881 atagttccat aattaacaaa tgaaccacct aaaacttccc aaatatatgt ttgtgctaat
     8941 acatagttat ccattgaacc actagaatag tagccaaaat aaccaattaa agaaatcttt
     9001 tttctaatag cacttgtcgt aattaaattt tcgagtggtg tcacatcata ttgaataccc
     9061 gcactcgtga ttgcgccttc ttgaatgcaa aaaacatctt ctccgtcaat ttgccagcga
     9121 accatgtaat catgagtata cattgttcct tcaggagtaa ttcgagtcat attcacatct
     9181 ttacgaccat tttcagtcaa tgttagattg gtagcacgtg tctgtgcatt cttttccaag
     9241 attgcttgcc attcttcaga ttcttgcgcc aatcgttctt gttcagaaaa agaagtcgct
     9301 ttttgaatat cttcaatggt tataaagtat ttctcataac cttcagggac ctcttgagtc
     9361 attacattgg gcggagaaac atcttctgtc gtgtcctgcg tagttgtctc ttctgttgtt
     9421 tcacttgtgg tttcagtact gatcgcttct tcactacttt ttgattcttc agtcttcatt
     9481 acttcttcag tagaatttga ttcatttatc gtttctgcaa atgccgttac agatcctaac
     9541 atgggattga gaaatgtaac gaaaattgcc aaccaagttg ccattctttt aaacatattt
     9601 ttttcgttcc tttcatttta tttttcagtc ttctaaagta atttcttatg gacttcgaca
     9661 ccgaaacatt tttatcctcc tcaaaacaaa aaaagactag aacttataat tctagtcttc
     9721 atcaaccact tacttttctt ttatttttag tagaaatcta cagtgtgttt ttgtgacccg
     9781 tcactgtaaa taataacatc caccataaaa cctttgttgt accactgcga atttggatcg
     9841 ctaacctctg cattagccca atcataggct tcatctacag tgttaaatac ctttccacta
     9901 ttaccaatct ctccaactac aattgttggc tcttcaggtg tcgttgtgcc accatttccg
     9961 ccttgatcgc ctccaccagg attgcttggt tcttcaggcg ttgttgttcc tccacctgtg
    10021 cttgggttag aaggagttgt ttctcctcca gtatttccac cattacctgg ttgtgtaggt
    10081 gtttgagtag tgccaccatt gttttctggt gtacttggcg ttgttgtacc accagttgtt
    10141 cctgtttgat ctccagctgg tgttgttggt gtcgttgtat ttggtgtcgt ttgtgtattt
    10201 gaagtcgttc cttgtcctac tggttgattc ggatcaactt tgtctttctc ttgaatcact
    10261 gtttgagcca cagtttcacc tgcgctgttt tgtacacttg ctacattgcc attaaatact
    10321 aatttatttc ctgcatagat taaatccaca tttgtaatct tattcatgtc cgctaatttt
    10381 tggacagtaa tatttgttgc ttgactgatt gcgcttaggg tatctcccca gacaattgtg
    10441 tattcattcc ctttaatttc tgctttgatt tgttccgccg aacgaggtgt ccaaactttg
    10501 ttttctgctg cttcagcatt cgttcccaca gcaagactaa ctcctccgac tacgattcct
    10561 agtgccaata ctttcaacat tttcattgtt tttcgcctcc gatttgaata aatttattta
    10621 caaaaacata gtaccaaact tatgtctatc agtcgatata ttttttgtag atttttttat
    10681 tttttgatat actaactttg gtactaaaat aactaaaagg aatgtcacac atgcaactta
    10741 ataatttaaa tgaaatacat gattgggctc aaaataattt acttaatcgt aaacaagctg
    10801 ccaaaatcac tggacaaaac tatgatgctt tttctagctc tttacgtaga aataatatca
    10861 aaccatttct agaaataggc gacgaacctg gtccttccat cattcgctta tatctaagag
    10921 aagatattga aaaatacgca aaacagctcg aagaaaaaag acagaaacgt caatgaggcg
    10981 ttttctgtct tggatttaca agtaaaacaa taataaacac ttttacaacg gaggttctat
    11041 gaataagaaa tacattgttt cagttggttc aatcgtactg attgtactcg ctattattgc
    11101 tttttctgta tccaacaaaa cagaaacaca gccacaaaat acggttcaat cgactactac
    11161 aagtcaatct agcgatcatt ccgtctatcc ttcactaaat agtttaaaag aaacgcatgg
    11221 acaagattta attgatcttg gttcttctca aattactact gtcttatttg aaaagaaaaa
    11281 tattgcgttt attactccta aagaaggaac tgaccttgaa ttttctatca aagtaacaaa
    11341 taccaaagaa aatttattct ccattcctga cattcaatat acccctaaca agttaaagat
    11401 tggggatacg tttggtttgg ctaaagtaaa tgaacaatac tttgcttact cactgaaata
    11461 agtttctaat agcaagagga aaaaccctct tgttattttt atcacataat cattttataa
    11521 aaacgcttcg aaaatatcaa catttttgaa gaaactattt ttctaatttc tcaatttctt
    11581 ccaagggatt ttcgctaatg acccatacag gcacagattg ctctcgaggc tccccttctt
    11641 ctaaccacca acctgcataa tgaacttctt cctcctcagc attaccatga taccaaccaa
    11701 caaaggattc atctacaacg aatgaaattt cttcttctgg atcaaataat tcatttttcc
    11761 atgcttcaat tgcttgttct ttggtgtact tttgtttgtt aaatacacca caaatttcgt
    11821 catctacata aaaaatttca aaatcaaaat tactcgttcc ttttactggc atcttaactt
    11881 ttcctccttt gactcacttc aatagatata ccactcatgt tcattccatt gtttactagt
    11941 taattggagt tttatcttag ggctttgtgc actctttcca aaaacttgag cgtcgttttt
    12001 ttcttctaac caaaaaaggt aatttcttac attcggtact aaatgttttt cctgcatttc
    12061 ttcatgaaaa acatataatt ctgcttcatt tttcaaccaa ttcattattc attccctcat
    12121 tcacttcttc ataccgccat tcagatagtg agatttcttt ttgtttctcc acttgtccta
    12181 taaaaatcgc tgtattcaac aagctgatca cacttaataa taaagcaatg aaactaatgg
    12241 ttacacttac tctttcacat tttgtcattt tgtttccttc tttactcgtt tgttatttct
    12301 tgtaataggt ctaataactc cttcaccgaa atttgcaaat ggttcgcaaa aatttcaata
    12361 aaagcactcg cacacattac cagaccttca actgatatat cactgcgaca ataaaattct
    12421 ggagaaacac tgatttctga ttctttctta caattgaacg aaaaaactat ttttgaatca
    12481 aattccggta attcttccac aactatgttt tgggattcta ccaagaaagg gagtgcctgt
    12541 tctaatgctt cttctggtgt ttgagcagat agaataattg cttcttgttc ttttatttca
    12601 ttgttctcaa atactttata actttttttc attgttagtt cctctttcat tcctctctca
    12661 attagctaag ccgttactgg tacgccattc tctaaattta gtatcgctaa tccttaatgc
    12721 tcgtcgaata tcaatcattc gataacctat tgcacgaagt tctttatatc ttttagcagt
    12781 tagcgtctct aacgtcattt caggcaatgg gttcgaatca aaaatagaat tcgaaccgat
    12841 tatttgggcc aattgttgta cttgtaacag ctcttctgaa cttaattcag tgattgctcg
    12901 attgcctatc attgcataca ttttctgtcg aatatacttc ttttcttgtt tagacaacat
    12961 ttctatccac tcctattttc attatcattt gttacctcca cttcagtaaa tccacaaata
    13021 aatgaattca tattccaagt accattccct tcaactacaa cttttctcct gtcctctttc
    13081 ggatattcaa gaatcagacc gtttaccaat atgtttttca ctactactaa tttgtttgta
    13141 taccgtggaa ttgctgttcc gatatacttc actttatttc ctacgctaat tgtcatttcc
    13201 tcactcctat tcttttttat ccaaaacttt ttcctgtcct attcctgcca acgaatatac
    13261 ttcactgcat gcttaaacaa aacaaaatgt tgccctgttt tatttccgtc ttttacttgt
    13321 tccacataca gttcatattt ggtcacacct tttaaaacgc cagatacttt ttttcatcca
    13381 ttgtcacaat tagttttttc atcaatcgat tgatactgca ctgctcattt ttcttgttca
    13441 tatagtattc ctttcttttt caaaactagc ctcttttgtt tactagtttt catcaatcat
    13501 caacgattta acagattttt tctttcgcag ggtttctagc gtcagcaacc ccaaactcta
    13561 aaaaagtgcc tttgctccct tgaaaaacga gcggacgatt gtactcgtca tcatatgttt
    13621 ttcatactac ctgatccacg caaataacct ttttttgttc tgtgccacac ccatccatac
    13681 gtatcgcttg agattcacca tactttttta ggtggtttat ccttcctaag tccctgttag
    13741 acacgctgat taacttagaa agtcctgttt gtatccaatt ttcaaagatc tcccaaaaga
    13801 aataagtagg tttacgtgta tcgcttctgt tcaaagtacc tgtttgtact tgagtatttc
    13861 tactcccctc gttcaaggga ttccttccac cagctagctt ttcctttcat catcaaatgt
    13921 tcctgaaaag catttacgtt tcgtgtccgt gtgcttactt attctttttt cgatagcgat
    13981 tatccgctcg ctactcgcta ttttatactt tgtttgttag ctcaaataaa aactaacaaa
    14041 caaaaaaggc tagtttcttt ccgttttatt tttttaactg tcgataaata gttgttagtt
    14101 taattccaag ttctaaaacc aatacgccca acaaaaccac aggaaaaaca attggggcaa
    14161 acaagtaaaa tcggatagtt ttcatgtctt ccctctttct ttttggtgtc gctgtggtcg
    14221 ttcttgccgt tcaatacttc gatacatttt ttattcatta tttgctcgtt ttgttttcct
    14281 ctggactgct tttcttgctt tttgaatact ttatacgctt ttcgtgcctg aattaatagg
    14341 tcttgtcggc gtttcattcg tgagaaaaca atatttgaaa ccatcaaatc ctggttcgaa
    14401 caaccacgat tcgcaataat tttcccatca aaaaaaatag ttttcacttg agaaaaaaac
    14461 agttggaaat cttgttttgt tgctaatttt tttgtcaaaa tcactgtgct tttccctgtt
    14521 gaatctacaa tgattcttcg aacaatccga ttcgataact tccaacgttg ccgattaaac
    14581 aatgttactt cacaacaaat atgacgtaag tgattagggg tcattttcat gtgtccaatt
    14641 cctccaacca attatgcatc gaaattttgg gcaattgtga cagcccataa atctgcgttt
    14701 ccaggtcttc ataactacaa gaacgcttgg cttgaagtaa tgaatattct gcttgatctg
    14761 atagttttgt tgccattgga tctagattca attctcttac acaacattcc caaaaattca
    14821 atagcgctct aaaataaaat ccttttcgat tctgaattgc ttttccttca tgacgagcaa
    14881 ttttttcatt ttgaatacac cgttgatacg ttttgcttaa atcttctgca aatgtttccc
    14941 ctaaaattcg atactcgctt ccatcagata aaccatgtgt ttgattatac tttcgttgcc
    15001 gcttttctat aattcgttta ctgtcgtata tcatgtttag ctgctctttc gcttcacgcc
    15061 attcagctga actgcgaaca atccatgtca aaacatcttt cggaacaaac gtactttcgc
    15121 tgtaatgagc aataaactct ttttgccaat catcagccat ttcaacagga ctagtcgtat
    15181 cgatttttgt atctagattt tctgtatcta gattgttaga agaattatta aataattgtg
    15241 cagaattttc acatggattt ttttcgtcaa aatcatcgaa aatcgctaaa ttatcaacag
    15301 attcctcctc ttcttttcct tgtgcagaat ctacactggt tgagtcaaca ggacctaatg
    15361 aatacacatc attttctgtc gtatctaatt ctcctagata caaccgagct ttgtttcttt
    15421 ttcctgtttg ttgatcaaat cccatatcct ctatttctag tagctcatat tctttcaatt
    15481 cttttttgat tgtattaaca gaacgtctgc aacaattcaa tgcattggct aattcttgtt
    15541 ccgtaaattc aaaataaaca aaaccttgac tatctacttg atttcgttga atggctaaat
    15601 ctaagcgtga ccgaaaaacc atatacgcga tttttgcttt atcagacaat gagcgatatt
    15661 tttcacttgt aataaatacc tttggaaatt gatagtataa ttcattgtat ttacgagttg
    15721 cggtaaatct ttttgttgcc atcgttatta cacctcatct tgtccatata ttaagaaaga
    15781 attttggtgt tggtagacac gaacatcttc cattgtagca atcaattctt ttagataaat
    15841 tcgaccgaat tcaattgtta gtaagtcgta atcttctaat tcatttttga tttcttcaat
    15901 ttcttcttca gacacattga gtaaaatggc taattcttga ttggtataag aaatatagac
    15961 atgtttcttt tcatcaatcc gtttttcaat taaggattct tctaagtact cattcagtaa
    16021 gctataggcg tattttgctt gtgcagtcat cgcataatat tttgacgatt ttataaagaa
    16081 tagttttggc atatgaaaag ttgcttgtgt cagcacatct gttgcgcgaa atttgttttt
    16141 taccatattt tttcctccta gaaaaacacg caaaaaaagc tctgtataaa tcgaattaca
    16201 cagagtttct tcttgttgat atttaatttc taaaatacgt ttcgggtaaa aactaaacac
    16261 ttactaatat ttagattgta gaatcttcgc cttcatttat tcattttaaa gagaaaaaga
    16321 tcaagtgaaa tgtgcccaaa agtttgattc tattaatttt taaacaccat tcataatata
    16381 tttcctataa tgtaaaaagc gtcgatactt attaaagcat cgacgttatt tttatagtaa
    16441 atttatcaaa atttttgcca acattttttc cgattttttt ccctatttct atctttcctt
    16501 atctttctta gttaatatta tttgatgtta atatatcact actaatatta aaaaacattg
    16561 att
//
LOCUS       DARBFO010000052        16360 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000052 DARBFO010000000
VERSION     DARBFO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.053"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..248
                     /locus_tag="SCK51_001865"
     CDS             <1..248
                     /locus_tag="SCK51_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1252491.1"
                     /translation="LKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEE
                     LKSAIDSYIYYYNNERIKQKLNWQSLVQFRKTTVTVV"
     gene            437..1105
                     /locus_tag="SCK51_001866"
     CDS             437..1105
                     /locus_tag="SCK51_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEG1252492.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1305..1691)
                     /locus_tag="SCK51_001867"
     CDS             complement(1305..1691)
                     /locus_tag="SCK51_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252493.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1663..3045)
                     /locus_tag="SCK51_001868"
     CDS             complement(1663..3045)
                     /locus_tag="SCK51_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEG1252494.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3201..4388)
                     /locus_tag="SCK51_001869"
     CDS             complement(3201..4388)
                     /locus_tag="SCK51_001869"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEG1252495.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4395..5426)
                     /locus_tag="SCK51_001870"
     CDS             complement(4395..5426)
                     /locus_tag="SCK51_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG1252496.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5442..6896)
                     /locus_tag="SCK51_001871"
     CDS             complement(5442..6896)
                     /locus_tag="SCK51_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1252497.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            7071..7976
                     /locus_tag="SCK51_001872"
     CDS             7071..7976
                     /locus_tag="SCK51_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEG1252498.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8067..8744
                     /locus_tag="SCK51_001873"
     CDS             8067..8744
                     /locus_tag="SCK51_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG1252499.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8741..8911
                     /locus_tag="SCK51_001874"
     CDS             8741..8911
                     /locus_tag="SCK51_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252500.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            9025..9921
                     /locus_tag="SCK51_001875"
     CDS             9025..9921
                     /locus_tag="SCK51_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1252501.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9976..11346)
                     /locus_tag="SCK51_001876"
     CDS             complement(9976..11346)
                     /locus_tag="SCK51_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1252502.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11584..12330
                     /locus_tag="SCK51_001877"
     CDS             11584..12330
                     /locus_tag="SCK51_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG1252503.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12327..13166
                     /locus_tag="SCK51_001878"
     CDS             12327..13166
                     /locus_tag="SCK51_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG1252504.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13167..14117
                     /locus_tag="SCK51_001879"
     CDS             13167..14117
                     /locus_tag="SCK51_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEG1252505.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14258..15010
                     /locus_tag="SCK51_001880"
     CDS             14258..15010
                     /locus_tag="SCK51_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252506.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15029..16180
                     /gene="queG"
                     /locus_tag="SCK51_001881"
     CDS             15029..16180
                     /gene="queG"
                     /locus_tag="SCK51_001881"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEG1252507.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5321 a         3044 c         3279 g         4716 t
ORIGIN      
        1 aattgaagga gtacaaaatc tttcaaagca tgtctcggaa agggaactgc ttagataatt
       61 cacttatgga gaattttttt ggcttattaa aacaagagat ttttcacggg aaagtttata
      121 actgtttcga ggaattaaaa tcagccatcg atagctatat ctattactac aataatgaac
      181 ggataaaaca gaaattaaac tggcaaagtc tcgtccaatt tcgtaaaaca accgtaacgg
      241 ttgtgtaaaa atatcatttt agagcataaa aaaaaggagt ggatttctcc actccaaaaa
      301 agtctaactt ttgggggtca ctacagtgtc acagtccttt ttttagtaaa aggatacatt
      361 tcttccttgt atattccatg cgtgttcgtg agaaagcata tataatttaa ttaacagata
      421 gttaggggga gaaaaaatga ttttttcaat tacaatgaaa ctggtgttcg ggttgatcgg
      481 tttattatta gtggtacgtt tgcttgggaa gaaatccatg tcagaaatca caccgtttga
      541 tttagtctat acgcttgttt taggtgggat cttagaagaa tcaacctatg atgacaatgt
      601 ccatgtaggg catgtcctat ttgccattgc gctttgggca gtaatgattt atggtatcga
      661 acgtatcgtc caaaaaaatg aaaaggtcaa tcgttgggtc aaaggcgagc catctgttct
      721 aatcaaagac ggcgtcatca acatgacaga gttgacaaat aaccatatcg agatggagca
      781 gttgcgagca atcttaagac agcaagaatg cttttctctt gaaaatgcca aacatgttat
      841 cctagagaat gctggtcaaa tgagtgtatt gaaaaaatca gatgaagaca aagctttatc
      901 gatcctgctt gtagatgaag ggcaaattca gcataaagtg cttcagtcca atcaattgac
      961 tgaagcatgg cttatggaga acttgaagaa agaaggctat gcagatgtaa aacagctgat
     1021 ttatgttgaa tggtcagaag aaaaagggct ctacatacta aataaagaag acgtcatgca
     1081 tgaatcttac cgaatagatg gatagataga cagaggttgt gaaatcagcg ccttggcttg
     1141 ttactcaaaa ctaagcgact ttttcacaac ctctgatatc taatatacgt tgttccagaa
     1201 gttgcttctt cgaaaaataa gccaaaattc cacaaaaatt taagaagcaa ttttcgaaaa
     1261 atcctttctt attttttgaa gttgatcact tctgtcacaa cctctcatgt cacaaaccat
     1321 ttttttgttt cctttaactc aatataccgt acgtcttccc acaatatcgg ctgatggtca
     1381 atcataaagt aatcttcata ggcttcacca atgaagtgcc cttcgacctc atccaagagt
     1441 cttccaaatt catcttttaa attgagctga atgattactg ctttacgaga taaaaaggct
     1501 tctgtcagta tactatcgat ttcttgcaat gtcatttgtt gtttaggaag aggctgtttg
     1561 cgtgcttctt cttcattttt tttgataccg ttgactagtt cttccatcgc aaaagcagtc
     1621 ccccatttta atccaaatgg ccgatctcga tactcgttat agtcaatgaa tttttttgct
     1681 gtccgtctca tcttttccac cttctaatcc actcatacca cctgcatgcc cgccaacgag
     1741 agaactacgt gcgatggcgc gtcctccttg caagagacta tgcgcatgga tgattgattg
     1801 aaagccgtat ttttttctaa tcgtatcgac aacaaaatca actttcgctt catttatctg
     1861 cttttcagga tcttcaaaaa ggttaagctg taaacctgtc gcgtatacga ggtcggaaca
     1921 gttcacgcct acacgccgaa tatcctggta gtggtaatgc tggtggaaaa tggtcaataa
     1981 agcatctgct agatctctgc tagcatttgt cgcttcgatt ttcatttgct ggtgaaaccc
     2041 ggtcttgcca tttggatcaa gatagcctaa agagtatcct acccataagc tgactacttg
     2101 tgtttttgct gcagatcgtc tcaagcgagt gccgacttgg tcggccattt ctttgataac
     2161 gatctcgatt tcttctcgtc ttgtataatc tctatctagg atttgactat ttccgatact
     2221 ttttgatttt ggtgtatatt cttgtcccaa aaaacttcga tcgatgcccc aagcatgggc
     2281 ataaagctga gtaccgatga cccctagctt gcttttcaag tgataataat ccgcgtgagc
     2341 taaatcataa acagaatgta tacccatttg tttcagacgt tttgctgttc gtttgccgat
     2401 tccccaaaag tcagtgatat ctgagatttt ccataacttt tctggtacat cttcataacg
     2461 ccattcagca aacatttgct ttccgtcttt tgagccattg tctaaggcta gtttagcaag
     2521 tagcgggtta tcgccaattc ctatagtcgt gtagattccg gtctgctcaa acacatcttt
     2581 atggatcagt ttagccatgt cgtacgcatt tgttttacca aagagtgcca tgctatttgt
     2641 tacatcaagg aagctttcat cgacggaata gacatgatgg ttcgcttcgt ctgcatattt
     2701 tttatagata gaattgattt ctctgttctt ttccatataa taggccatcc ttggggcaac
     2761 aatatataaa tcacttggat aggggaaggg aagatctctg gcacggctga tattggtgat
     2821 gccgtaagct ttttttgctg caggactgga agccaagatt aatccgctac cccgtgtttc
     2881 tgtcgaatcg gacggatagg acatgactac taatttagtt gtaaggggat caagatcctt
     2941 ttcaacgcac tctacggagg cgtaaaaaga tttgcagtca atacataaaa tatctcttga
     3001 aggttctttt tcataaacaa ataccggatt ttgattcgcc cacataacaa tcacctcttc
     3061 aacacaatta gaacacacgt tctctttccg tgtcaataaa aaacagcaat tttcaaatac
     3121 gagcaaaagg ctcacatttg gaaattgctg agaggttatt tttatctatt tgtttttagc
     3181 tgagatcgtt gattttttta tcaaacatga acagcttctg gatcgatctt tgttgtccca
     3241 tttccaatgt gtgcaacata aaattccgga gcatagccga caactttcat gtattcatca
     3301 tagacattgt ttttgaaggc aggtatattt tcttctttga caagagcaat cgcacagcca
     3361 ccaaatccag ctcctgtcat tcgtgcacct aagacacctt cttgtttttg agcagcatct
     3421 actagtgtat ctaattcgat cccagttact tcataatcat agcgaagaga gtggtgagaa
     3481 tcatttaaca gttgaccaaa tgcttctaag ttcccagctt ctaattcagc ttttgccttc
     3541 aaagtccgct gattttcaga aacggcatgg cgagctctgc gtatcaatgt atcatcgccg
     3601 atttggtcaa tagttgattc aaattcttct tcatctaagt ctcctaaaga agagataggc
     3661 aaagtttttt gcaaacgtgc caaggcttct tcgcattcgc tacggcgttc attgtatttt
     3721 gaatctgcca attcccgccg cttattcgta ttcatgatga cgattgcata accatctagt
     3781 ttcactggaa ccatttcgta tttcagcgta tttgtatcta ataagattgc atgatctagc
     3841 tcaccgaagc cgatggcaaa ctgatccatg atacctgaat tgacaccgat gaattcattt
     3901 tctacttttt tacctgtttg gactagttcc aagcgatctg tttttaaatc aaacagatct
     3961 tctaatacaa cacctactaa taattctaat gaagcgctac tggaaagtcc tgcaccatta
     4021 gggattgttc cttccactaa caactcaaac ccatgatcga ttgagtgtcc agcttctttt
     4081 aatttaagga ccattccttt tacgtaattt gcccagtttg cttttttatc atattctagc
     4141 tcatctaaag taaaactaat gacaccgata tcgtcaaagt ttgtggaata aactaatatt
     4201 ttattatcgt ctcgaggtgc tgctacacct tgcgttccat aagtgatgga agcggggaag
     4261 acatggcctc cattgtaatc tgtgtgttca ccaatcaaat tgatgcgtcc tggtgagaag
     4321 tattgtgtag tttttttagg tccgaatgtt tcggcaaatt ttgtgtttaa gcttcttttg
     4381 atttccatga tgagttattc ctttcttgtt ttgattttga attctgtcac tgtttcaaat
     4441 actgatccag cttttaaatg aatcgaacca aatgctgaga agcgctctgc acctggagct
     4501 gtttgagtct caaatgtgat gccaccgtga tgtgccagtt ttcggttgcg catttctggt
     4561 gtttctgtgc cgaaatttgc agtgaaaata accacactag aagcatctgt agcaatacct
     4621 acttggattc ttttatcggg gctagtcaag cgagcggctt ctttacccaa gccggtttct
     4681 tttaagaaaa atggatgatc gattccgtca acaagatttt tttgatccaa atcactagca
     4741 aaaacggacg ctaatttttt aggtatttga aaatcgaaag cacttcctgc agcattttct
     4801 tttaccccta taggaatact atctgttcgc aaaggagcat aagcttcgct attgagccaa
     4861 agctcatgtt gatcgattga ctgagaagca tctcccgtca agttgaagta aacatgattt
     4921 gtcggattga ataaagtatc ttggtcactg gtgccgcgag aggtcactcg ccagatatta
     4981 tcttttgtca aagtatagcg gatttccaca tccattgtac ctggaaaacc atgttctccg
     5041 tcgggacttg tagtagtaaa aatgacagaa gcttcatttt cgccattcaa aatgacataa
     5101 gaccattttt ttaattcaaa tccaggagca ccaccatgca gattatgtcc agtttttgga
     5161 tctgtggcta attggtaagt ttttccattg agagaaaagc ggccattttc gattctccct
     5221 gcggtgcgac cgatagaagc accgatatac ggatcttttt cgatatattc ttcagcggtg
     5281 tcaaatccca aaactaattc tcgatcattt gattttaaac tgacgattct agcacctaaa
     5341 tcagagatag aaagtgctag cttgtttttg ttctccaagg ttatcaagtg ataaccttgg
     5401 ccaaaatctt tttctgtaat cttcattttc aagatccttt cttaatggga aggaactact
     5461 gcatcgtgaa gttcatcttc cattgtatca gctggagcca atttgttttc caattcctca
     5521 acgatttgtg catgtttttt ctctgtcaat gtaatcttcc aagcaaaaat aatagcggag
     5581 aaaatcatca aagcagcagg agcatagaat gcataagctt tgaaagtcgt tacgccatga
     5641 gcagtgatat cagcagcagt agcattacca gtcatcccag aagcgatagc gacaaaccct
     5701 acgatcccgt tagacaaagc accagcgatt ttatctagta aagggcgaat tgatagagta
     5761 acagcttcgt tacggatacc atttttccat tgaccatatt caacagagtc tgtgatcgtc
     5821 atcaaagcgg ataagaaaat caattgttga gggaagtaga acaatactag cccgatgata
     5881 acgattggca aacttgaacc tgcgatcgta aagaaaaagt aagaagaaat catcattgcg
     5941 atccctccaa ggtaaacgta gcgacgagag atgacgcgtg taagcattgg gaatagtgga
     6001 acagctacta aaccaagtaa ggcagcaact agaccaacga tccaaaattc agttggttgg
     6061 ccaagtacat atttgaagaa gtagaataat acggcagttg ttgctacatt ggcaatcgca
     6121 taagcgatat aagcaagggc aagccacatc aactgatcat tttgagcaac tgctttgaac
     6181 acatctttga tcccagcttt ttcattttgc tgacggatcg cagtatcgac ttcttttgtg
     6241 cccaaggcag tgataatcgc agtaccgcct gaaataacag cagcaatcac accgaaccag
     6301 aaccagcctg aagcgccttc aatatgttta tgagtaacaa gataggtaaa gaaagtaacg
     6361 atagggacaa cgactagagt agtaccattt gcacctagag aagagccgaa acgagcaaac
     6421 gtagcaaatt tttcacgttc ctttgtttcc gtactcaaag ctggaatcat tgaccaaaaa
     6481 gcgatatcct taaatgaata aaaactatcc aataagatga aaacgattgc aaataatatg
     6541 ataaataaag tcgtattatg agtagctaag ccaaagaaat tggtaaagat tgctaccaag
     6601 agtacggaac taacaacgcc gccgataact aaccatggtt tgaatttacc gtattttgta
     6661 cgagtattat cgatgatgcc accaatcaaa ggatcaaaaa taatttcaac taagcggata
     6721 cctacaacta agcttgtaac tgtagcaatc atttttgcat tcgttgcctt atctgttcct
     6781 gcgaacatct ggcttgtcac gaataccata aaataagtgc ttaatgtaac atagaatgtg
     6841 tcgtgtccaa aagcgcctaa agcgtaggac acacgttgga tcattttgct tttcataatc
     6901 tgtattcctc tcctgttcaa tatataatac taagtaaacc ggttacaacg aataacaaag
     6961 atcttttaat gacttaaact taacaagaaa tcgcttcctt tttaatatat ttctgtgact
     7021 tttatttaac tttttgttgc attatactaa aggagtaagg agggatagac gtggagcaat
     7081 cgattttttt aaaaaaggaa catcaacaag tagaaaaaga attgtatttt gatttttgtg
     7141 gtttctctaa aacgatgccg tttcattctt ttggtcctgc tattcgcaat gattacatcc
     7201 tgcatgtcgt gatggaaggc aaaggatctt atcatgtgaa aaatcaacaa ttccaattga
     7261 aaaaaggtga tctgttcttg attcgtccag gagactcgac gttttactta tcagatggag
     7321 aagatccgtg gatgtattgc tggctttcat ttggtgggag tgctgcagaa gaaatcattc
     7381 ggcagtcgct tttcagagaa gaccaataca cgatggtctc ttctgacata caaaaatata
     7441 tcgacattat tttagaatgc atgaaagata cggaagaaac cttggaaaat gaattacagt
     7501 taacagcatt aacctaccgt tttttatcag tccttttaaa agatggcgga aaggtcagcg
     7561 gtggtgtaca aaaggttcat tcctctttag ctgttgaaac agtcaaatac atcgaggcac
     7621 attatgctga aaaattgtct gttgaggaaa tcgcccagca tttagcggtc aatcggagcc
     7681 atttgtcgcg agtattcaaa aatcatatgg gtaccagtat caaggaatat ttgatcgggg
     7741 tacggatcaa tcgaggggcg tttcttcttt ctctgacgga tgaatccgtg gaaagtattg
     7801 cctatcaagt agggttcaat agtctagtcg tatttagccg aatgttcaaa aagagtaccg
     7861 gagaaacggc tacgaactat cgtaaacgta tgaaaaacga aaattttaag aatattgctt
     7921 tggatacctt gaaaaaacag ttggaaaaac aaaccgcagt ttcttggaca acctgattag
     7981 tctggttaaa atgacaagca tcggcaaaag aaacttgtgg cggttatgct acactgtaaa
     8041 gcgaagacta aaaaggagga aaagggatgc gtacacttat catcattagc catccaacga
     8101 tcgaagaatc tttaaatcag caatttttca aagaagcttc tgctggttta ccagctactt
     8161 ggcatcattt ggaatcaagt tatccagacg gaaaaataga tatagagact gaactacagc
     8221 tcttggccca gcatgatcgg atcattttcc agtttccgtt ttactggtat agttctcctg
     8281 ctcatttaaa aatttggcaa gataatgtct tggaacgagc agcacatgta ttaaaaggga
     8341 aagaactagg aatcgtattg acaacaggtg tggcggaaaa agaataccaa gctggaggaa
     8401 aagaagaata tacgatttcc gaatttttga ggccgtatca gcgaatcgca aataaattcc
     8461 atatgaccta tcttccgcca ttcgttttag cacaatttat gtatttatcc caggaaaaac
     8521 gttgggagaa gctaattgct tatcagcagt atctctcatt ggaaggcaaa ccatcgctga
     8581 ctcagcggat tgactggttt attcaaagag tacaggaaaa tcagaaaatg caggaagaag
     8641 aatctgaaaa acaagcatat atcgtagaag cactgactga ggcaaaagag cagatcgaag
     8701 acttgacctt tacactgcaa gaaatgaaag gaacttcatt atgacaaatg attggctgat
     8761 ccaacaatta gatgaattaa gcgaaaaatc agaatatcaa gaccgtgcgc tcttatatga
     8821 aataaagaaa ctgatggctg agatagacga acgaacaatg cagctgcaag gagaaatcga
     8881 cggacaatct tggaatcatc tcaactggtg aaattttctt ataaataaca aagataaaaa
     8941 tgtgcacagc gacaattatt tgctacaata agaatatgaa gcgtttgata acgctattat
     9001 acataaaaat tggtggtgaa aaaaatgtat gatgaaaatg agtggggaga aaatgttcgg
     9061 atgacccgaa gaagacagca aaaacaggca gctcgtaagc ggatgatcat gattggggca
     9121 gcagttgccg tgtttttgat catagcagga ggagttgcgg gtctatattt tcatggaaaa
     9181 aattcgtcag aagcgaaaag tacgaccata acggaaacaa cgacaaagaa aacagagacg
     9241 aaacaaacaa gtgagtcatc aactaaaaca aaagagacac aaacttctga agaaaaaaca
     9301 gcaaatggtt tgccagagca acttcaagca atcgtaaatg attattcgaa aaagatcgaa
     9361 gaaaagacgc ctgcattgat tgaagaatac cagacagaaa ttcagggaaa tcaagaggga
     9421 atagctggtt tatcagcagt tgctaaccaa aaagcacgtg agcttcaagc aatctccgac
     9481 gaagggatca gaaaattacg tgcagcgtat caggcagcag aaaataaaga cggtgtagac
     9541 ttagatactt tgatcaatca attatcggct aattatacga atcatgtcgc taagatttcc
     9601 gatatttatt tgcagactag cgcctctctt caagcagagt caacaagcac acaagacaca
     9661 acatcttctg attctgagac agcagagtcg gtagaagctt ctgaaatgca agctcgaaca
     9721 acacaagaca caacagattc ctctgaaact aataccaaca gcgcgacttc acaggctgcg
     9781 acaaccgtag ttttggaagg agaaggtcca aatcaaatag ccgaacgaac tggcgtacca
     9841 gtagaaacga tcttgtcgct gaatgggatg agcatggaga attatttcct gactcccggt
     9901 gagacgctaa aattgaattg ataaaagcaa taaaaaagaa cgaagcttcc actaaggtga
     9961 agcttcgttc tttttttatt ttccggttct tttttcgcgt acaaagtaat ggatcgtcat
    10021 gtaaagcaag aaaacgagtc caataatcaa tgagattctc gtttcaggat tgataaacat
    10081 gaaaatcaat acgatagcta aaaaagcaag tgtcacataa ttcgtataag gagatagtgg
    10141 cattttaaaa ggatgatcat gcattttatc tgcgtgcttc tttttgaagc gaatctcgct
    10201 gatcaaaatc actacccaag gaaccatacc aggcaaaata cttgagctgt atacgaaaac
    10261 gaaaatattt tctgactggt tgatgaacat tggtagaaga taatttaaga tcagaccaat
    10321 taagattcca cctgagattg ctacgacagg ataaaaaggc gcaccgtgac gagatagttt
    10381 cagaaatttt ttagacatct ttccatttat ggcaagtgta tagaccatac gacttgcgct
    10441 gaaaatccct gaattgcaac ctgataatgc cgctgtaatc acgacaaaat taatcagtcc
    10501 tgcagcaaat gtaatgccga ctttagaaaa agtttgtaca aaaggtgaac caatctcact
    10561 caattgattc cacggataga tactcacaat gataaaaata gcaccaatat aaaaaatcaa
    10621 gattcgaccg atagtggact ggacagcttc aatgatcgtc tttttaggat tctctgcttc
    10681 accagcagtc attccgatca gttcgattcc ttgataggaa gcaacaacga tcgataatgc
    10741 aaagaaaaat cctttgacgc ctccagtgaa gaatccacca tttttccaaa gatttgaaat
    10801 ccctactgcc tgaccatgat taccaaatcc aaataagatt accccaagac ctgcaatgat
    10861 catcaaaatg atcgttatga ctttgatgat cgaaaaccaa aattctaatt cgccaaacgc
    10921 cttgacagaa ataagatttg ccagcataag aaacaagacg acaatgattc cgggaataat
    10981 tgccggcaaa tcaggccacc aataattcat ataagatcct acagcaatca cttcacttat
    11041 tcctacgaca atatattgaa aaatattact ccaagcggtc aagtagccag ccacaggatg
    11101 gatgtactcg ctcgcgtaat ttgcaaaaga accagtcgaa ggatcaacgt aaagcatttc
    11161 gcctaaagca cgcataatca aatacaaaaa gaatcctgca atcgcatagg ctcccatcac
    11221 tgagggccct gtccaacgta tggttgagga ggcacccata aacagaccga caccgatcgt
    11281 tccacctagt gcgatcattt ggatatgacg ggcactaaga cttcgagtta acttttcttc
    11341 ttccatacaa aaactccttt cgttgttgag tcaacaagaa aaaataatca aacgtttcaa
    11401 cacatgtttt tcattattag gttagcctta ttgaactctt gatgtgattc tacagaaaat
    11461 tttcataaaa tgcgaggaaa attcgaaaaa aactttttca tcattctact tgaaaatgct
    11521 ttccatttta gttaaactat atttaaataa aaagttaggt aaacctaaaa aggagtgtaa
    11581 gatttggaac gtacggccat tgtaattcaa gacctttcag ccgcttatca agggaaaact
    11641 gttttgaaaa ctatcaactt gacgataaat ccacaaaaaa ttaccggtat tatcggacca
    11701 aacggtgcag gaaaatcaac atttatgaaa tctttgctag agttgatacc agctactact
    11761 ggtaaagtca ccttttctgg acagccggta aactcagtcc gtaaaaaaat agcctacgtg
    11821 gaacagcgaa gtgaattgga tctttccttt ccaattgatg tattaggcgt tgtactttta
    11881 ggaacatatc catctttacg aattggacaa agacctggga aaaaagaaaa agaacgtgca
    11941 agacaagctt tgaaaaaagt agggatggaa gaatatgcca aaagacagat cagcgaacta
    12001 tcgggtggac agcttcagag agtttttatt gcaagagctc tagcccaagg agcagaatgg
    12061 atctttttag atgaaccatt cgtagggatt gatgcgttaa gtgaacgaaa gatctttgac
    12121 atcttgcagg aattgaagaa ttcaggaaaa acgattttga tcgtccatca tgatcttcat
    12181 aaagtagaag aatatttcga tgaggttatt cttttaaata aacagctgat cgcttccggt
    12241 ccagtacaag aagtctttac atcgaagaac cttcaattgg cctatggtga gatcatcaga
    12301 catttagtaa aaggaggaga gaaaaaatga tccaatcctt tattgatgga ttaatgaatt
    12361 atcaattcct gcaatacgct ttggtcactt ccatgcttgt aggacttgct tctggagtca
    12421 tcggttcttt tattatttta cgagggatgt ctttgatggg agacgctatc tctcacgcag
    12481 ttcttccagg tgtagccatt tcttatatgt ttggttttag ttacatcttt ggagccactg
    12541 cttttgggat gctaacagca gcagtgattg gtttcgtgac tcagcggagc cgattgaaaa
    12601 atgatacagc gattggaatt gtctttagct ccttttttgc attagggatt atcttgattt
    12661 catatgccca gagtgcgaca gatttgtatc atattttatt tgggaatgtc ttagctgtta
    12721 gggaaagcga tttgttgctg acagcactcg ttagtggaat cgtcttgata tttgtctttt
    12781 tcttttataa agaattgaag atcacatcat tcgatccgac aatggcaaaa gcttatgggc
    12841 tgaacacatc gctgatccat tatcttttga tgttcttttt gacattagtg gctgtagtca
    12901 gtttacagac agtaggaaca atcttggtga ttgccatgtt gatcacacca gccgccacgg
    12961 cttacttgct aacgaaccat ttactgaaaa tgatcattac agctgcagga atcggtatgc
    13021 taagtgcagt tgtcggtgtg tttttcagtt atagttacaa ttggccatca ggagctacga
    13081 tcgtgttagc atgtaccgca ttttttatcc ttgctttttt attttctcca acaaaaggaa
    13141 ttttatttca gaaaggaagc agatagatga aaacaaaaaa atcactcttt ttaatcttgg
    13201 cagttagttt cttggtatta gccggttgcg gaaagcaggc ttctgaccaa gcggatgaag
    13261 gaagtaaaga aaagttgtca gtcgtggcta ccaattcgat cttggcggac atggcaaaag
    13321 aggtcggaac agatcaaata gatatccaca gtatcgtacc agtcggaaca gatccgcatg
    13381 aatatgaagt actaccagaa gacatcaaaa aggcaagcga tgcagatgtt attttataca
    13441 acggtttgaa tcttgaaaca ggtaacagct ggttcgataa cttgatggaa acggctaaaa
    13501 aagaagaagg aaaagattat tttgcagtta gcaaaaatgt agaacctctt tatttaacta
    13561 gcggtgaaga acatacaaaa gcagatcccc atgcttggct agacctatct aacggaataa
    13621 aatatgtgga ggaaatcgca cgtatattct ctgaaaaaga tgcagaaaat gcgacactct
    13681 ataaaaaaaa tgcagaagca tatgtggaaa aactaaaaga attagataca caagcgaagg
    13741 aaagttttgc ttctatcgaa gagaacaaaa aattattagt aacaagtgaa ggggcattca
    13801 agtatttttc acgagcatat gatttgccag cagcttatat atgggagatc aatacagaaa
    13861 gtcaaggtac gcctgatcaa atgaaagcga tcattgatca gataagagca aaagaagtac
    13921 cggttttatt cgtggaaacc agtgtagact caagaagcat ggaacgggta gcaaaagaaa
    13981 cgggtttgaa aatctatgat aaactgttca ctgattccat agcaaaagaa ggagaacaag
    14041 gagattctta ctatcagatg atgaaatgga atattgaaac gatccatgaa ggtttgtcac
    14101 aaacaaaaga aagctgaaaa gtgcaaggtc tagcttgagg agcagacctt gcatttttta
    14161 taagagatat gaaaaaaaat tatgctgttt ttcgtcgtac agataccttt tttgaagaaa
    14221 ttccgttata gttagagtaa ggaaggaagg tgtggctatg aattttacac gtaaagataa
    14281 atatacgatt aaagaagaag tctttcgatt gatcagccag caggaaaata ccgacaagga
    14341 tcaagacctt caatttcttg aagcagaatt agctaaatta ttcatagcaa atcaaaatga
    14401 gacaacagaa ccagatgaag agctgtacat aggcaaatgg ttgtttgatg aatttcagcc
    14461 tttagatgaa aataccggta tcggttattt tgtgttcctt tctccagtcg gcttcacacc
    14521 tggcgtatta gttaaacaaa acgacgaaat aaccgtcaaa gcatggcctg cattattttc
    14581 agtcatcaaa gcagaaggag taatcaaaaa gatccagtcg gcctattata aaagtcaatt
    14641 tattgccaac agcaaattaa tgaatatgtt ttataatcgt ctagtagaaa ttgctaggcc
    14701 tgggaaaatc ccgcaaacat tcacgtacac acagcaaaaa aatctctatg aagaagtatt
    14761 gtattcccgc gatctgtctg agttttcgcc atgggaagag gtaaggctcc atgaactgtt
    14821 aagtacaaaa ccagtttcat tgaatttttt taatgcatta gataacaaaa gaaaattaat
    14881 ttcaacaaat tattgggaag tatttgctgc aagaaaaaaa tggatggaag aaaagaagat
    14941 tgccgatcag cagatccagt ttgtccagct gaaaaaagaa aaactatatt atgggttata
    15001 cagccgttaa cagcaagcga ggaagaccat gacattaaaa gaagaaatca tacaagaaag
    15061 caaacgatta ggaattgaca aaatcgggtt caccacggcg gaaccttttg attcattaaa
    15121 agaatcgttg gaagaacaaa aagcagctgg ccatacttct ggctttgaac atccaaatat
    15181 cgatgagcgt ctttatccag aaatgacatt tgatcagccg aaatcgatca tttccattgc
    15241 acttgcgtat cctactagga tccatgaaga agtgcctagg gatgaaaaac gcgggcagtt
    15301 tgctcgtgct tcttggggga tcgattacca tgatatactc agagaccgtt tgaaccgatt
    15361 gatcgaattc atccaaacaa gagcagaaaa atggcaaaaa gaagaggaat ggcgtctagc
    15421 tccgcaagta gatactggag aactgatcga tgtcgctgta gcacagcgag ctggtttagg
    15481 atttattgga cgaaacggat tgttgatcac tgaagagttc ggttcatttg tctatcttgg
    15541 tgagatcgtt actaatattg tatttgagcc tgataaacca ggagaatttg gttgcggtga
    15601 ctgtatgcgc tgcgtgactg cttgtccgac aaaagcattg cttggtgacg gaagaatgaa
    15661 tgcccagcga tgtttgtctt atcaaactca gacaaaaggg atgatgccgg aagaatatcg
    15721 taaaaaaatg cgaaatgtca tttatggatg tgatatctgc cagttagtct gcccttacaa
    15781 tcaaggaaaa gattttcatt ttcatgaaga gatggaacca aaagtagacg aagtatatcc
    15841 aaaattaaag ccaatgcttt cgatgtctaa taaagacttc aaacagcaat ttgggcattt
    15901 agctggttca tggcgtggga agaaaccttt gcagagaaac gcattgattg ctttagctaa
    15961 tctaggtgat cgaacggcat tgcctgagat ttcagaatgt ttaacagatg tccgccctgt
    16021 catcagagga acagcagctt gggctattgg acgtctagga acaaaagaac cggaaaaatg
    16081 gcttgatgta ttagatgaaa tattagctaa agaaacagaa gaagtcgtta taaacgaatt
    16141 ggaacatgcc cagaaattat tgcggagaaa gttaaaataa aaaaatagtt aacattattc
    16201 aaagtttttg tccagtaatt gttgtaacaa ttactggttt ttttgtattt tttacatttt
    16261 tttaagagaa tttgataaaa atcaaaaatt tttttgcttt tttttatgga ttattgcagt
    16321 gaatgttaca gaatctgcgt ttttcattca aaaataaaaa
//
LOCUS       DARBFO010000053        16165 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000053 DARBFO010000000
VERSION     DARBFO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.054"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            79..1125
                     /locus_tag="SCK51_001882"
     CDS             79..1125
                     /locus_tag="SCK51_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEG1252508.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="SCK51_001883"
     CDS             1474..2106
                     /locus_tag="SCK51_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HEG1252509.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="SCK51_001884"
     CDS             2694..4133
                     /locus_tag="SCK51_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1252510.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="SCK51_001885"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="SCK51_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="SCK51_001886"
     CDS             4895..5110
                     /locus_tag="SCK51_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252511.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="SCK51_001887"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="SCK51_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5770..6681)
                     /locus_tag="SCK51_001888"
     CDS             complement(5770..6681)
                     /locus_tag="SCK51_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1252512.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="SCK51_001889"
     CDS             complement(6700..7704)
                     /locus_tag="SCK51_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEG1252513.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="SCK51_001890"
     CDS             complement(7701..9824)
                     /locus_tag="SCK51_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1252514.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..12276)
                     /locus_tag="SCK51_001891"
     CDS             complement(9829..12276)
                     /locus_tag="SCK51_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1252515.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12263..12652)
                     /locus_tag="SCK51_001892"
     CDS             complement(12263..12652)
                     /locus_tag="SCK51_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1252516.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12712..13215)
                     /locus_tag="SCK51_001893"
     CDS             complement(12712..13215)
                     /locus_tag="SCK51_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG1252517.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13228..13452)
                     /locus_tag="SCK51_001894"
     CDS             complement(13228..13452)
                     /locus_tag="SCK51_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252518.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13556..15466)
                     /gene="ltrA"
                     /locus_tag="SCK51_001895"
     CDS             complement(13556..15466)
                     /gene="ltrA"
                     /locus_tag="SCK51_001895"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEG1252519.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         4791 a         3203 c         2884 g         5287 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    10801 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    10861 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaatccttga ccccttgtga
    10921 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    10981 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    11041 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    11101 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    11161 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    11221 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    11281 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    11341 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    11401 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    11461 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    11521 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    11581 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    11641 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    11701 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    11761 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    11821 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    11881 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    11941 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    12001 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    12061 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    12121 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    12181 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    12241 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    12301 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    12361 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    12421 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    12481 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    12541 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    12601 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    12661 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    12721 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    12781 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    12841 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    12901 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    12961 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    13021 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    13081 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    13141 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    13201 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    13261 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    13321 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    13381 aattgccagt aagactaagg attgggcatt ggttaaaaac cattgatata tggataggta
    13441 agttcctgat attataaata tctcaaactt ttccccccat tcacacggag cgtgcgactt
    13501 tcaccgcact ccgcgttcca tcgtgtttat attgaaaagt atttatgtag tagacctata
    13561 tcaagtttaa ttcttgattg ccagctaata ctctatattt atttagtctt atgagctgga
    13621 ctttatttag cttcaataga gctatatatt tttgtattgt ttcttctgtt ttagcgtgta
    13681 ttagacggtg taccgcctct tttaccaaaa taagattttt ataatcgtca tttccaccag
    13741 ctccagttgg tacaatatga tgacaatgta tttcattagc ttgttgaaat tcttccagag
    13801 ttatagcaca ttttccatgt tgagcagaaa atagcgaaat tcgattgtct gcgtattcta
    13861 ttgtgcgatt ttccaaagat aatttcatta actggtacat cagtaattta tcaacactta
    13921 gattggtgtg gataaatttt cttccctcta gagtgtagca attcgctaaa tacacctttg
    13981 ccattggttt tctgtgttgg atatatccaa cagggaaaat aggttcgccc tcgccagcaa
    14041 cataccgaag cattttagat ttaccatatc ttgttgcctc ctttgtcgta agttgtctgc
    14101 ctgtttttct caatctgccc cttttgtttg tacctaatct gttagtgaag atagccatta
    14161 cagctctatt cagtttcata caatccagat ttacattcgt tgcaatctga taataatttt
    14221 gcatacctaa taccattgaa ttataaagtc ttgtttcttc taattccgtt ttcccttttg
    14281 agggagaagc aattttcttt gcctgtgata ccagcttgtc tttttggttt ttgaattgtt
    14341 tatcacttat atgtgattta acaactaact tctttcttct cggtatcatt ttaatcttga
    14401 aaccaagaaa gtcggagtat ctttttctaa catttacaat tcttgttttt tcttgtgata
    14461 cttccaattt taatcgttgt tcaatccatt gagtgattgc tattttggct ttttcagcac
    14521 tttccttgta gcggcagaaa actctaaaat catctgcata acgaaccata tacatttcct
    14581 ttaactttgt tttcttcatt tctttgtaag cgttgctttt acatgggcta ccgcttttat
    14641 tgatatgaac cacatagttt tttgtaacag gattttcctg ccattggctt tctacccaat
    14701 ggtctaactc attaagaaca atgtttgcta gtagtgggga tattattccc ccttgcggag
    14761 ttcctttctc tggatatgtt attgtaccgt tagtcatttt gattggagct tttaatatcc
    14821 tttttaaaat gaatatcaag tgcttgtccc gtattcccat agcccatatc tgttttatca
    14881 gcttggaatg gttcacgttg tcaaagaaac ccttaatatc aaattcaatc acataatgta
    14941 gctggctgat ttgtaggcgg ttataggttg cttttattgc attttcaact gacctattag
    15001 gtctaaaacc atagctgttt tcactaaatt tagcttcgca gattggttct aatacttgtt
    15061 ttatacattg ttgtataagc ctgtcccaca tacaaggaat acctagaggt ctggttttac
    15121 catttggttt tggtatttct tttcgcctca ctggcttagg tcgataccca tgtggactgc
    15181 ctgcaacaat gtatcttact tttttgacaa ctgtttctgc tggcaatttg ccaatatctt
    15241 taattattgt accgtctgtt cctgctgttt tactgcctgc atttgctttg atatttcgat
    15301 atgccagtaa tatattttct ctcgatagga taatatccat aagatgagtg aaaatatctc
    15361 cattcttact tttggcatat aaatcatcaa aggtgttttg catttcgtaa tattcagcgt
    15421 gtcttaaatc ttctacacac aatgtttcat ttttattttt aggcataagg cttcactcct
    15481 ttacttgaaa ttgagtgagc ttttttatct tactcgtaat ccttattgaa acaacataat
    15541 acttttctaa tatatacacg acttgagggc attcctccat tctcattaca agaatttctt
    15601 aggttcgccc tctgcttttc agcattagtt caactgctta ttgttcttcg tcagtttatt
    15661 ctcttgatga gaacccaata cctttcctcg ttccgataat cttatctata catatatatc
    15721 cttaggtcac tagctataag cctgctggct tgataccgcc tgtaacgtta tatggcattt
    15781 cataacctat attcttactt tgccacacca gctacgattt ctcgtacctt tgcatttcta
    15841 caaagttcaa atttagaccc gtacattcgc tgtttcgtca gttccttata gaacattcta
    15901 accatagata ttttatagac ccccgacctg ttggatacgc tacccacctc tggatagttt
    15961 tcgatagaag atatatgttc atcaactatt acggtatctt tcagccgtct ttaccgagct
    16021 tcaaacattc tgagtttcct catacaatgc ttgtcggagg ctcagggcag gcgtttcaga
    16081 gcgttacttc atcattccac ctgtcagatt atcagttctc ctaataacca aactttttcg
    16141 ttcttttatt agtgccttag ctttt
//
LOCUS       DARBFO010000054        16091 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000054 DARBFO010000000
VERSION     DARBFO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.055"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            138..1475
                     /locus_tag="SCK51_001896"
     CDS             138..1475
                     /locus_tag="SCK51_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEG1252520.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="SCK51_001897"
     CDS             1523..3652
                     /locus_tag="SCK51_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEG1252521.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="SCK51_001898"
     CDS             3627..4316
                     /locus_tag="SCK51_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252522.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="SCK51_001899"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="SCK51_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="SCK51_001900"
     CDS             complement(5546..5740)
                     /locus_tag="SCK51_001900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252523.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="SCK51_001901"
     CDS             complement(5790..6230)
                     /locus_tag="SCK51_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEG1252524.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="SCK51_001902"
     CDS             complement(6234..7079)
                     /locus_tag="SCK51_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEG1252525.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="SCK51_001903"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="SCK51_001903"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEG1252526.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="SCK51_001904"
     CDS             complement(8703..9650)
                     /locus_tag="SCK51_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG1252527.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="SCK51_001905"
     CDS             complement(9873..10223)
                     /locus_tag="SCK51_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEG1252528.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="SCK51_001906"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="SCK51_001906"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEG1252529.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="SCK51_001907"
     CDS             11646..11966
                     /locus_tag="SCK51_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1252530.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="SCK51_001908"
     CDS             11988..12452
                     /locus_tag="SCK51_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1252531.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="SCK51_001909"
     CDS             12657..13262
                     /locus_tag="SCK51_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEG1252532.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="SCK51_001910"
     CDS             complement(13301..14590)
                     /locus_tag="SCK51_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEG1252533.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="SCK51_001911"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="SCK51_001911"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEG1252534.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15700..16091
                     /locus_tag="SCK51_001912"
                     /pseudo
     CDS             15700..16091
                     /locus_tag="SCK51_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336126.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT         5079 a         2950 c         3282 g         4780 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga tatggatgat attgaagaaa actatctaaa tggactggga
    15781 aaacaaatat atttcagata cttaagctat ctaaaaatga aataagacat agtaattttt
    15841 tagctttttt gtttgctcca aatgaaaccc acaatttatc tgatgagttt tttaaaatgt
    15901 ttttgaaaag gtatatagat tctaatgatg ataccaaagc tgctatcaat tattttgatg
    15961 ctttacttaa tagctatgaa gatttaattg tttacagaga aaataagaat attgatattt
    16021 tattggtatc ggaaaaaaat aaaatagtgg tgtgcatcga aaataaaatt ctgtcttcgg
    16081 agtctggagg g
//
LOCUS       DARBFO010000055        15948 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000055 DARBFO010000000
VERSION     DARBFO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.056"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(387..809)
                     /locus_tag="SCK51_001913"
     CDS             complement(387..809)
                     /locus_tag="SCK51_001913"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEG1252535.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(823..2229)
                     /gene="atpD"
                     /locus_tag="SCK51_001914"
     CDS             complement(823..2229)
                     /gene="atpD"
                     /locus_tag="SCK51_001914"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEG1252536.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2252..3154)
                     /locus_tag="SCK51_001915"
     CDS             complement(2252..3154)
                     /locus_tag="SCK51_001915"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEG1252537.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3168..4724)
                     /locus_tag="SCK51_001916"
     CDS             complement(3168..4724)
                     /locus_tag="SCK51_001916"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEG1252538.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4747..5289)
                     /locus_tag="SCK51_001917"
     CDS             complement(4747..5289)
                     /locus_tag="SCK51_001917"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEG1252539.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5276..5800)
                     /gene="atpF"
                     /locus_tag="SCK51_001918"
     CDS             complement(5276..5800)
                     /gene="atpF"
                     /locus_tag="SCK51_001918"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEG1252540.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5891..6106)
                     /gene="atpE"
                     /locus_tag="SCK51_001919"
     CDS             complement(5891..6106)
                     /gene="atpE"
                     /locus_tag="SCK51_001919"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEG1252541.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6159..6878)
                     /gene="atpB"
                     /locus_tag="SCK51_001920"
     CDS             complement(6159..6878)
                     /gene="atpB"
                     /locus_tag="SCK51_001920"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEG1252542.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7180..10023)
                     /gene="rnr"
                     /locus_tag="SCK51_001921"
                     /pseudo
     CDS             complement(7180..10023)
                     /gene="rnr"
                     /locus_tag="SCK51_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(10026..10781)
                     /locus_tag="SCK51_001922"
     CDS             complement(10026..10781)
                     /locus_tag="SCK51_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1252543.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10884..11120)
                     /gene="secG"
                     /locus_tag="SCK51_001923"
     CDS             complement(10884..11120)
                     /gene="secG"
                     /locus_tag="SCK51_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEG1252544.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11238..11516)
                     /locus_tag="SCK51_001924"
     CDS             complement(11238..11516)
                     /locus_tag="SCK51_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEG1252545.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11629..12597)
                     /locus_tag="SCK51_001925"
     CDS             complement(11629..12597)
                     /locus_tag="SCK51_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1252546.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12812..12856
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12880..13854
                     /locus_tag="SCK51_001926"
     CDS             12880..13854
                     /locus_tag="SCK51_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEG1252547.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13859..14434
                     /locus_tag="SCK51_001927"
     CDS             13859..14434
                     /locus_tag="SCK51_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1252548.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14501..15640)
                     /locus_tag="SCK51_001928"
     CDS             complement(14501..15640)
                     /locus_tag="SCK51_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEG1252549.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4356 a         3443 c         2856 g         5293 t
ORIGIN      
        1 ctgagccaga atgtggttca ttctgactca gatttgattt tgcacaactc atcagtcctt
       61 attgacaaag agcccaaaag tcttgtcgca gtcccttatt ccaaataaac ggtgttcaat
      121 catctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttccatttg
      181 tttctctaat actcaggaca aaacgctgat ttcacaacct ccgatatacg gtgttccaga
      241 agtagcttct tcgaaaaata agctgagatt tcacaaaaat ttgagaagca attttcgtaa
      301 attccttctt atgtcttgaa gctgaccact tctgtcataa cctcttttta ctgttcttaa
      361 acacggataa acatccgcat ctcattttat gagtgcttag atactttgat acgattgatt
      421 gctcggtgca acgcaacagt tgcccgtttc aattcgttcg tatcttgttt tgcttttgct
      481 tcttcgattt ggcgttctgc gcgttgtttt gcacgttccg cacgagatac atcgatatca
      541 cgttcacgtt ccgcactatc agcaacgatt gaaacaacat tatcacgaac ttccatgatt
      601 ccgccattaa cagcgatcca atcgacgtgg gtatctgaat ctgttcgttt tacacgaact
      661 tcatcaatcg tcaatggaac gatgattggt gcatgtttag gcaaaatccc aagctcacca
      721 gctgtcgtac gtgcaacgac gatagctgca tggtgatcat agaccaaacc ggcaggagtt
      781 accacattta ctgttaaata ttgatccatg gggcacccct ttctagtagc ctagtgtttt
      841 cgctttttct acgacgtctt cgattcggcc aacacttcgg aaagcttctt caggaagatc
      901 atcgtatttg ccttccaaga tttcgcggaa tcctttcact gtttcttcta cagggacata
      961 tgaacctgga agaccagtaa attgttcagc cacgttaaag ttttgtgaca agaagaattg
     1021 gactctgcgg gcacgagaaa ctaacacttt ttcttgatcg gataattcat ccatacctaa
     1081 gatagcaatg atatcttgta attcacgata tcgttgcaat acgtgttgga cttctgttgc
     1141 tactttataa tgttcttctc ccacgatttc aggtgctaaa gcacttgaag aagaagccaa
     1201 tggatctacc gctggataaa tcccttgttc tgttaattta cgttccaagt tggttgtcgc
     1261 atccaaatgg gcgaatgctg ttgccggcgc cgggtcagtg tagtcatcgg ctggcacata
     1321 gattgcttgg atcgatgtga tcgaaccttt tttcgtagat gtgatacgtt cttgcaattg
     1381 acccatttct gttgccaatg ttggttgata accaaccgca gaaggcatcc gtcctaacaa
     1441 ggctgatact tcagatccag cttgagtgaa acggaaaatg ttgtcgataa acaacagtac
     1501 atcttgacct tctacatcac ggaaatattc cgcaatcgtc aaaccagtca aagcaacacg
     1561 catacgtgca cctggtggtt cgttcatttg tccgaacacc atggctgttt tttcgataac
     1621 tcctgaatct ttcatttcat aatacaagtc attaccttca cgagtacgtt cacctacacc
     1681 ggtaaataca gaaataccac catgttcttg ggcgatatta tggatcagtt cttggatcag
     1741 tacggtttta ccaacaccgg caccaccgaa tagtccgact ttcccacctt ttaaatatgg
     1801 ggcaagcaaa tcgataactt tgatccctgt ttccaaaatt tctgtacttg tacttaattc
     1861 gtcaaaggct ggtgcttttt tatgaatttc gcttctttcc gcatcttctg ggaaaggtgt
     1921 ttctaaatcg atcgtatctc ctagtacgtt gaatacgcga cctaatgttt ctttacctac
     1981 aggaacagag attgctttgc ctgtatcaat gacttccatt cctctttgta aaccatctgt
     2041 tgattccatc gcaatggtcc ggatcactcc gtcccctaat tctaacgctg cttcaagaac
     2101 aacttttgat ttattttcgt catttttata aacaactaat gcgttgttga tatctggtaa
     2161 ggattggtct aaagaaaatt ccacgtcgac aacgggaccg attacttgaa caatcttgcc
     2221 tgaactcatc ttttttcctc caatctcaat actattctag tgccgaagct ccggcaacaa
     2281 tttcggtaat ttcttgggta atagcccctt gtcttgcacg gttgtaagaa accgtcagct
     2341 catcaatgat attagcagca ttatcagttg ctgttttcat tgctgtcata ccagcagcat
     2401 gttctgctgt tttggcgtca acgatcgcac cgtagatcaa actttcagca tactgaggca
     2461 ataactgaga caagatttct tcttttgaag gttcaaaaat atattcttgt tcgaaagtag
     2521 ttgcttcttc tggatctaag tctgagattg gcagcatttt ttctacacga aactgactag
     2581 ttagcgaatt gatatgatgg ttataacata cgtataattc atcgaatact tcgttttggt
     2641 acatagttgt tgccatattt acgattttgc ggacttcatc aaagcttggc tgatcagata
     2701 gattgcgcag ctcatatgct agattgatac cgcgtgcttt aaagaagtct gcaccagttc
     2761 cgccaattgc gatcatcacg taatcatcgg gtgattggtg gtcttcttct aaaatagaca
     2821 tcgtttgttt caatatcgag ctgttatatc caccgactaa accgccatct gcagtgatga
     2881 cgatataacc cgttttctta acaggacggc tgagcaacat gctgttgtaa ttgatatccc
     2941 ctctaggatt ttctgaggca atatcattta actgagtagc agtcaaatgc gtaacgattt
     3001 ctcttacttt tgttgcataa acctgaaact tctgtgaaga agcttcagat tttgtcaact
     3061 tagaagcaga caccatctgc attgcccgcg tgatttggct cgtttttttc gttgaagcga
     3121 tacgtgtctt gatttcattt aatgaagcac ccattcatct cacctcacta tgcattttga
     3181 atggatttca atttatcttc tgtagaggaa ttatttcctt ttgtagctaa gaacatgtct
     3241 ttatattcct gaatcgcact gttcaacgct tcttcttctg gaagatcttt tgttgtacgg
     3301 attgtttcaa agatatctga atgattggta tccagatatt cgaataattc atgttcgaag
     3361 tctaaaatac tgtctacagg gatactatct aagaatccat gtgtcaaagc atacaaaatc
     3421 actacttgtt tttcaacagc tagcggtgca tgcaattttt gtttcaagat ttctactgta
     3481 cggcgtccac gatttagttt tgcttgtgtt gctgcatcca agtcagaacc gaattgtgta
     3541 aatgcttcta attcacggta acttgctaag tctagacgta aagtcccagc aacttttttc
     3601 attgctttga tttgcgcaga accccctaca cgagatacag atagtccggc atcaaccgct
     3661 ggacgagtac ctgcgtagaa caagtcgctt tctaagaaga tttgtccatc agtgatcgaa
     3721 ataacgtttg ttgggatata agcagagata tctccagctt gtgtttcaac aaatggtaag
     3781 gcagtcattg aaccgccacc taaatcatca cttaattttg ctgcacgttc caataagcgt
     3841 gagtgtaagt agaaaacatc ccctggataa gcttcacgac ctggtggacg gcgaagcagc
     3901 aaggaaagtt cacgataagc aacagcttgt tttgaaagat catcaaaaat gatcaagaca
     3961 tgtttcccgt tgtacatgaa ctcttcaccc attgcagtac cagcatatgg tgcgatataa
     4021 agcaacggcg ctggttgaga cgcaccggca ttcacaacga ttgtgtaatc cattgcgcca
     4081 tattttttca atgtttcaac ttgtgtacga actgtagaat ctttttgtcc gattgctaca
     4141 tagatacaga tcatatcttg acctttttgg ttgatgatcg tatcgatcgc aatggaagtt
     4201 ttccctgttt tacggtcacc gataactaat tcacgttggc cgcgtccgat tggtacaaga
     4261 gcgtcgattg cttttagacc tgtttgcatt ggttcgttaa cagatttacg ttgcataacg
     4321 cctggtgcca ttgcttctac tggacgagct ttgtcagtaa cgatttcacc tagtccgtca
     4381 attggctgac ctagcggatt aactacccgt ccaatcaagg cctctccaac tggaacttcc
     4441 atgatctttc ctgttcgttt tactttatct ccttcacgaa tggactcgaa atcgccaagg
     4501 ataatgatac ctacatcatt tgattctaag ttttgcgcca ttccgtatga gccatttgaa
     4561 aattcaagca actcaccact catcgcgttt tctaaaccat gtgcacgagc gatcccgtca
     4621 cctacataag ttacggtacc gatttcttcg actgaaagca cgttttgata attttcaatt
     4681 tgttctttaa tcaaggcact aatttcttct gctttgatgg ccattcgatt cacctacctc
     4741 tttgtactat ctatttaatt gattacgcat attttctaat tgcgtgcgaa tgcttccgtc
     4801 gattactcga tggttagctt caacaatcgc gccaccaata atagaagaat caacgatttg
     4861 ttttaattcg actgattgat agttcattgt ttttgcaaca ttcttttcta attgtattcg
     4921 ttgttcgtct gaaagtggaa ctgctgttgt gacacttccc aacagcaaac cttgatgttc
     4981 atcatatcga cgttcatatt cttcgatcat gaacaaaaga tcatccattc ggttatacgc
     5041 ataaacaact tctaaaaagt ttttaacgat tccatcatag ccactaacaa gcttgtccat
     5101 gatttctcgt ttttcatgag gttctagacg aacatcactt aacatattac ctaatccagg
     5161 tacttgttca tagatttgac gtaagcttac gagatcttga taaatttctt ctgctttatt
     5221 tgaatcgatc gccagttcaa ataaagcttt cccgtaacgt cttcctactg tatatttatc
     5281 tagtttcatt tgaagaacct agaccttcga tatattgatt gattaatgat tcgtgcattt
     5341 ctggagataa ttctttgttc aagatttttt ccgcgatttg aagagatagt tctgcaacat
     5401 cgtcttttac agagttcagc gctgaatcac gttctacagt gatatcagct tgggctttac
     5461 tttttagacg agctgcttct tcttgagcat ccttcaaaat attttggcgg cttaattctc
     5521 cactttcttt cgcatttttg atgatatcag ctgcatcaga acgagaggct aacaattgtt
     5581 gttcgcgttc ttgttccatt ttcgctgagt tgatgcgaga ttgttctgca gaatccaaat
     5641 cattggcgat cttgtcttca cgttttttca aaatatcgct gattggtccc caagcaaaat
     5701 gcttcaagag agccagtagt atcaaaaatg agccgctgac aacgatgaca tttcctaaca
     5761 tcgcattgcc aacttctgca attgccaatt gattcagcat aggcgagcct tcctttcttt
     5821 gaagtacttc tagttttcat gtaaaaaagc agtaacaaac caaattgttt ctgccttaat
     5881 actgattcaa ttaaacacca aaaactagaa tcaaggcgac aactacccct aagataggaa
     5941 cagcttcgac tagagctacc ccgataaaca ttgttgtacg gaattgacca gacatttcag
     6001 gttggcgagc cattgattcg attgttttag agataacttg accgttacca taaccggctc
     6061 cgattgctgc tcccatgatt gcgattgctg ctgcgatata attcataatt gttttcctcc
     6121 taaattagat aaatagtagt ttgtttttaa aacgaatatt attcttccac ttcaatctta
     6181 tggctcatat aaaccataga taaagtaacg aagacaaatg cttggatgct tccgataaac
     6241 aaggagaatg cgatccagac catctctaat ggaattgcta acggcaatga gaaccagcct
     6301 aagcttatta ccatttttgc aatcaaggtt agcaaaactt cccccgcata aatattccca
     6361 taaagacgta aagcaagtgt taataagttt gtaaattctt caatcagttt catcggaaac
     6421 ataaaactat atggtttcaa gaacgaattt acgaaatatc ctttgaagcc taactttttc
     6481 accccaaata agtgagtaag taagatcatt ataaatgcca aagttaaagt aacaaacggg
     6541 tctgctgtcg ggcttttcca aagtgttgtt tcgttaggta aaacgatttt tgttactaaa
     6601 ccgatgttgt ttgcaacaaa aacaaataaa aacaatgtaa atgccaacaa atggaaattg
     6661 ctcaattctt ttctaggcag attatctgtt acgattcctc gactaaaatc aatgacccat
     6721 tctatcgcat tttgtttgcc tgttggtttc aacttaagat ttcttgtaaa aaagtaagct
     6781 aagaagaaaa caatcaaaca ggtcagtaac accattatac aaactgtacc atcaaaccaa
     6841 attggtccaa tgtggaacgt tagcgagcgt tcatccaatc catttcacct cttttccccg
     6901 aaattcacat aaaatcaatt catgtgcgta tctggttttt tccaatcggt ttctaaccac
     6961 acggtatcat accaccatga aacagaaaat tcaaaacatt ttcatgaaaa ttagacactt
     7021 ttttatttca gtcaaaaacg aagcgaattt ttctttatac acggtgctcc tgatctaaga
     7081 aaaaacctat gataatcatg ttttatcaga ttaaatgcta acaaatttta tcattaagta
     7141 aaagcaaaag caccgaatat gcgaaaaagg ataatgaaat tatctagaga aattttttaa
     7201 tgttcgatca atttgtcgat cgatatcttt tcgtttcaaa tcttcccgtt tatcataaga
     7261 ttttttccct ttcgccagac caatcaatac cttggcatag ccgttacgaa tatagacttt
     7321 taaaggaaca acagtgattc cagtattttt caattcattt tcaagccgga tgatttgttt
     7381 tttgtgtaat aataactttc tcgtgcgcaa cggatcatga ttaaaaatat ttccttgttc
     7441 ataaggactg atatgaacat tatgcaagaa agcttctccg ttgcggacgc gaataaatcc
     7501 atcttttaga ttgatccggc tgtttcgtat cgacttgatc tcggttcctt gcaagaccat
     7561 ccctgcttcc atcgtgtcga tgatcgaata atcatggcga gctttcttgt tttgtgcaat
     7621 taattttccc tcgccttttg gcatagctgt ttcctccaac tcattttatt ttttcttttt
     7681 cttcttttta acacctttgt aaaatggctt cttccctttt ttcttaccga acgtttgctt
     7741 actatcgcta tatcgtttgt tcttcttgcc atttctttct gggcgttttc ctgcttgttt
     7801 tccttcgcgt tttcctgatt ttccatttcg tgtttttggc cgttccaacg ctgtaacctc
     7861 ttctgcggaa acaagttcaa aatctactgc tcgttcatct ggatcagctt tgactacttt
     7921 tactcgcact ttctgtccga ttttaaaagt cagaccagtt ctttcaccta caagagctaa
     7981 atgattttca acaaaatgga agtagtcctg tttcaattca ttgatatgga tcaatccctc
     8041 tactgtattc ggcagttcga taaacaggcc gaatttcaca acagagctga tgatcccatc
     8101 gaattcttcc cccactttgt ccatcatgaa ttctgctttc ttcatggaat ctacttcacg
     8161 ttctgtttct actgcacgac gttccatctt agaactatga tctgcaattt caggaagttc
     8221 cgtagcccac gaactttgat tgctttcact tttatcttca ctgtaagaac ggatcagacg
     8281 atggacgatt agatcgggat agcgtcgaat aggagatgta aagtgtgtgt agtaatctgc
     8341 cgctaagcca taatgcccaa aattgtcttc tgaatagcgt gcttgttgca tgcttcttag
     8401 caacatagta ttgataactg cagcttctgg tttgtcttct actccttcga tcacatgctg
     8461 taaatctttt ggtgtgatcg ttcctttcgt tcctcggacc agaatgccca aagctgaagc
     8521 aaaatcaaag aagcgctgca ttttttcttc ttttggctgt tcatggattc ggtaaataaa
     8581 cggcagattt aatttattat aatgttcagc taccgtttca ttggccgcaa gcatgaaaga
     8641 ttcgatcaag cgttcaccaa ttcctctgct tcttagctgg atatcatttg ggtgtccttc
     8701 agcatcaaca aggatttttg cttcgcgatc ctcaaaagaa atcgcgccac gtttgattcg
     8761 catcgtttca aggattcgat gcaattcacc catttcttgg aacataggta cgagctgagc
     8821 atagcgttgc atcgtttctt cgtcttgatc ttctaagatt tgattgacag ctgtgtaggt
     8881 catccgttct gttgtttgga tcacacttgg gaaaatatca tgagagacta cttgtccatt
     8941 tggatcaatc tccatttcgc aactcatggt cagccttgga acatggggat tcagtgaaca
     9001 gataccattc gataaacgtt gcgggatcat cggaatcact cgatcagtca aatatacact
     9061 tgtccctctt tcatatgctt ctctgtccaa ttgactgcct tgtgtcacat agtaagaaac
     9121 atctgcaatg tgtactccaa gaaaataatt accgttatct aattttttaa ccgtaactgc
     9181 atcatctaga tcttttgcat cttctccatc aatcgtaaca atgagctgat ttcttaaatc
     9241 tctacgtcca gttatatcct tttcgctgat ttgatctgga acttcatctg cttcagacaa
     9301 tacatcatca ggaaatttcg tagggatacc attagccacg acaattgaga ggatatccat
     9361 gccagggtca ttcttatgac caatcacttg cttgattatc ccttctaaac tagtcgcata
     9421 ccctttttca gggtaatggg tcagttcgat cagcacaata cttccgtcaa ctggacgaac
     9481 tccctctgct gctgcatata ctttaaattg gttgagcttt ctatctttcg ggataacaac
     9541 gccgtaaaga tccgtttctt gtatttcttc ttctgaataa gcaacgaatt cccctgctaa
     9601 ctgagtagtc gcccggtctc gaatctcgac tactttcccc tctgctccgc gatccatatc
     9661 tgtatttgct cgcttcatga tatcgattgc tacggtgtct ccatccattg caaaaccagt
     9721 cgcttctttt gggatataga tgtcatcttc ttcgggatca atcgttacaa atccaaatcc
     9781 tcgttcattt gctcgaaatg ttccttcgat caagattggt tttaaaggaa gtttcacttt
     9841 tcctttttta ttgaaaacta tactctgttc tctttccatt tgagcaatcg tttgtactag
     9901 taatttaaaa tcatcgcttt tttggaaccc taaaccttca gcgatttctt ccatagaaaa
     9961 actgcgtttg cgactgcttt ccatgaaaaa gagaattttc tcttttatcg tttcttttgt
    10021 catatttatt cctcattcca aggtaatttt tctaaaaatt ccaagacgtc tttttctaat
    10081 tcgcgtcttg ctgtaccaac tgtaatcaca tgaccacttt caggatacca tcgaagtgtc
    10141 actcgctgac ggctcaactt gcgtgcagtt tcaaatacgc cattagggtc gatcatttca
    10201 tctttcccag cttgagctaa aaatatcggc tgttggatat ctgacaacct actttctacg
    10261 attgctgcct gatcttgtat atccattaat tgttgttcag ctaacgagcg gtaagcaact
    10321 gctttttcat tgatttcttt ctctgattta cctgctgtct tcagcacttg acgtacgtat
    10381 ttttcaaagt tttcaggcac gtttgttttt acaggagaca aaggcgaaca gaaaaaaccg
    10441 ccgccaatca ctgattcttc ttctagtgct cgtacagcaa agatgccgcc cattgataat
    10501 cctaatacgg caatatcaga aaactgctca gactgtaaaa aatggatggc ttttttagtg
    10561 tctgcccacc agctttctgc tttttcttgc aaaatatcat atggctccat tgttccgtgt
    10621 cctttaaaaa gcggcgcata aactgtataa tctgcttttt ccaaaaatcg tgcgagcatt
    10681 cgaacatcgt ttggacttcc tgagtaagcg tgaagcagca aaaccgctcg ctttccatgt
    10741 tttgcataaa ggggttttgg taattgtctc actcgtctca cctctttcgc ctcattttac
    10801 catagtttct ttcataactt cttcttaagc gttctctttg caataaaaaa agccgcccgc
    10861 cgttttgcga gaggcttatt caactacttc gaagataaat atgctaggac aaataataag
    10921 atcatccaga tagctcccat aactgcagtt gatcgctgca tgacagcttc aaagccgcga
    10981 gctttttgtt tgccgaatag ttgatccgca ccgccggtaa atgcactcgc tgcactattt
    11041 tgcttgcttg gctgcatcat aatcgcaatg accataacaa tcgataaaac gatcacgatt
    11101 cccaaaatta aattatacat atcgcagcct ccttcccaaa agatatcgtc tctttaactt
    11161 tatcatagat aaaagtccgt gacaactcta tataaatgga ataacagctg aaagttcaaa
    11221 actttcagct gttattctta tgactgacgt aagacgtctt gatattctgg tttcttttca
    11281 aactcttttt ttgcataagg gcataaagga atgatctttt tcccgtctcg tcttgctaat
    11341 tctactccag tatacactag cttttcagct aacttttgac cacgatatgc tggatctaca
    11401 aatgtatggt caatgatcat gatgtctggg cccgcatctg accatgtcat ttctccgatc
    11461 tcttgttgtt catcattgaa taaagcaaga cgattttttt cttctttgat ttccatatgc
    11521 cgcactcctc aaataactga tattttttta ttcctacctt attttagcag tctcctgatc
    11581 tataaacaat tatttatcct cgaattactt acaaaaaaga agagaaaatt aattcatcgt
    11641 tattctttgt tcaataaatg aaaaaatcac tgtccaatag gttttcggat ctttcatcga
    11701 tgcttccaag tgttctgctt ctggaacaag cagcagtgct ttatcccctg ccgtagcatt
    11761 ttgaatcgtg tacgctgctt ctgtagggac aaaatgatct ctttcaccat gaatgaccaa
    11821 taaaggcaga tggttgctgc caagctgtct cgtagctgat gcttgcttta aggagtaacc
    11881 ggcgtatttc tgggcataat gattcgcata tctcatgacc atttttttag gaaagacagt
    11941 gattttactt aacatatatc ggaattctgc ataaacagaa acatatccgc tgtctaaaat
    12001 aaatcctttg accgctgatg gaagtttttc accacttgcc atcatgatcg tagaagctcc
    12061 cattgatccg ccgtgtaaaa taatggacgc attcggctgt tcatctaaga tgagttggat
    12121 ccaggcaatt aaatctagcc gatctagcca tcccattccg atgatttctc cttcactttc
    12181 tccgtgcgca cgtaaatcag gtacaagaac gttgaatcct ttctctgcat atcttttacc
    12241 tatatgagac atgtctcttt tccctgtgct tcggtaatca tgtacgcaaa tgacccattt
    12301 attctgctta tttggatgga tgaacatctg accagctagt ttcaacccgt cataactttt
    12361 gattgttttg ggtgttccct gctcccaaaa gctttcacac tcttcttcta atttttgtcg
    12421 ttttttttct actgacgtga ctgcagcagg gtccatcaaa cgatgaccag tgtcttcata
    12481 ccagtcatat cctctctgta ctcccgctct tactaatttc tttgcataaa aataagaaag
    12541 aaaatggtaa ctgatcccta taattatcag aataagaatg aaataaaaaa taaccattgt
    12601 acacccgccg ctttctgcca aactataatc taactattat ccaatgtacc gatccatagg
    12661 attctttatt ttcggcacac gtaaattcca tttctttttc attgtatcat ttacttaaag
    12721 ttctcctcaa tagatttact tgtaagagag catttacatt cgcaataaaa gccagtatac
    12781 taatttcgtg cattataatc aagacgctcg tcttggactg gttcggaaac ttcccagaat
    12841 aaaaaactaa gtatctctaa agaaaggaga ttggaaataa tgcgaaaagt aattattgat
    12901 tgcgatccag ggatcgacga cactttagct ttatcattag ctgtcaaatc ccctgatatc
    12961 gaagtagttg ctattaccgt agtttgtggg aatgtccctg cagatatagg tacgcagaat
    13021 gtgctgaaat gcctggaacg ctgtgatcgt ttagacattc ctgtttatca aggaatggaa
    13081 aaacctttga agcaaccttt catcagtgca caagacactc atggtatgga tggtttaggg
    13141 gaaacaaatt tcccgatgat cttgagaaaa caggcagaac ctctccatgc cgttgatttt
    13201 ttggctgatt atttcaaaga aaagacaaat acatcagtca ttgctttggg tcctttgacc
    13261 aatatcgctt ttgctttgaa agtcaaccca aatattggga aacatatgga tcgttttgtc
    13321 tcgatgggtg gaacttacaa aagccatggt aactgttcgc cagttgctga atacaattac
    13381 tggtgtgatc ctgaagcagc ttcttatgta ttcgagaatc tcaaacaaac gatagaaatg
    13441 gttgggttag atgtgacaag agaaatcgtc ttgacaccga ctatcctcga atactgctgt
    13501 caaatgaatc ctgaagaggg agagtatcta aaagcgatca ctcgttttta ttttgacttc
    13561 cactggaaac aagaaagaat tttaggatgt gtcatcaatg atccgttagc tgttgcttat
    13621 tttatcgagg gggccatctg tgaaggtttc aaaagcttca ctgctgtgga aactcaagga
    13681 ataagtcgtg gacaaacatt ggttgatcgt tatgaatttt ggcagaaacc tgctaacagt
    13741 aaaattatga caaaagtaga tactcgtctt tttttccgaa agtttttaac cgttctttta
    13801 aatgcacaag aagaaacgat tatgaaagat ttggaaaggt taaagatggg ctaacaaaat
    13861 gaataaaaac aaaatggcaa ctcgcgtact tacagtgatg gctttgtgta ttgggatcaa
    13921 ttatattgga ggaacgattg ctttatggct tcgtttgcca atctatctcg attctatcgg
    13981 aacgattttt gctggtgcat tgcttgggcc tgtacctgga atgctgacag gattgtccag
    14041 cggtatttta agtggagtga ctacagatat tttttctctg tattattcgc caatccaaat
    14101 tctcacaggc ttgcttgctg gattgatttt gccaagatat ctcggcaaag ataaaaacag
    14161 cttgaaactg ttattactcg ctttgatttt gtcggtacct ggaacgatcg tttcttcgat
    14221 cattaccgtt cagttatttg gcgggatcac ctcctcagga tccagtatga tcgttcagct
    14281 attacatggg ttaggtttaa gtcagacaac aagtgtgatc ttggtacagg caggtacaga
    14341 ctaccttgat cgtttgttgt ctatcgtcgt tgtttcatta gtcatcacta tgttgccaca
    14401 acgcttgata tattcaaaaa gaaaaatagg ctgaataaac agagctggat tagcaaaata
    14461 atgctaatct ggctctgttt tttcatttat taaaaaaatc ttacgctaag taggataaaa
    14521 taattccttg atatttttca atcatatcca aataattatc gatttcaaca tactcatcta
    14581 cttgatgagg caaagtaacg acaccaggcc caaagacgac aaagtcgaag gaatggttag
    14641 ctttcgtaaa ttcggctgca tccgttgttg ctgccgctcc aactaaaggt aatggctggc
    14701 tgaattgttg ctgaatgcaa tgaatcaaag gagaatctgg atcagctttg acaggtattt
    14761 tgttgtaatc gatcatcaat tccaaatgat agtctgtctc ttgattcaat tcattgacga
    14821 ttgattgcag caatgcaatg attttatcat ttgggtattc tggaatcgat cgaatgttcc
    14881 cttggagctg cgcatggctt gggatgctgt tgacttgatt cccgccactg atcaatgtaa
    14941 cattatggat tgtttttcct aggacaggat tctcgattgt ttcagcgagg tgattcattt
    15001 cagcatttgc tttcgtaatg aattcattta ggtgattgat tgcattataa ccttggtctg
    15061 gcatagagct atgcgcttct tttccgtgcg aggtgactgt atagttgatt gatcccatgt
    15121 gcgtatacat caaactgtaa ttagttggct ctccgatgat caaggcatct aagtcatcta
    15181 catagcctgc tttcgtgagc tgttccccac ctagttcacc cacttcttct ccgacagtgg
    15241 ctaataattt gactgttccg ttaaatggtt ttcctgactc tttcagttcg atcatggcaa
    15301 tcaccattgc tgccaagcca ctcttcatat ctgtagcacc acgtccgtac aaacggttcc
    15361 catggatttc tgctgcaaag ggtgcatagg tccaagacga ttcatcacca gccgccacga
    15421 catccatatg cccagatagg cctaacactt ttcctgattg gcccttttgg taacgagcaa
    15481 tcaaattatc tctaccatct ctgtaagaaa caatctctcc tgttatgtca tgctttgcca
    15541 aaagcttatt caaataggct gctacttcac cttcgtttcc attcactgat ttaattcgga
    15601 tgatctcttg taaaatcgcg attttttctt cttttttcat ctaatcctct cccttgccat
    15661 ttctttttat tgtatttgag tgtgctcgaa ttttcaaata ggagcaacca aagtaaaaat
    15721 gaagaaagag agccacttta tgaaaaatat gctgctactt ccctcctatt ttatttatac
    15781 gagaggactt gctcatagat tcctattcta ctgttctatc cttaaaagct cttatatgat
    15841 gctagttatt gttagctttc ctttttcaag gatgatcttt tcttgtacca ttctttgtaa
    15901 acatcatata cagaagctag ttgcaaaaaa aactctctac ctgctgat
//
LOCUS       DARBFO010000056        15715 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000056 DARBFO010000000
VERSION     DARBFO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.057"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(275..641)
                     /gene="ssrA"
                     /locus_tag="SCK51_001929"
     tmRNA           complement(275..641)
                     /gene="ssrA"
                     /locus_tag="SCK51_001929"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(812..1585)
                     /locus_tag="SCK51_001930"
     CDS             complement(812..1585)
                     /locus_tag="SCK51_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1252550.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1729..2079
                     /locus_tag="SCK51_001931"
     CDS             1729..2079
                     /locus_tag="SCK51_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEG1252551.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2060..2776
                     /locus_tag="SCK51_001932"
     CDS             2060..2776
                     /locus_tag="SCK51_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1252552.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2776..4224
                     /locus_tag="SCK51_001933"
     CDS             2776..4224
                     /locus_tag="SCK51_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1252553.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4294..4803)
                     /locus_tag="SCK51_001934"
     CDS             complement(4294..4803)
                     /locus_tag="SCK51_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252554.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4793..5518)
                     /locus_tag="SCK51_001935"
     CDS             complement(4793..5518)
                     /locus_tag="SCK51_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252555.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5741..7861)
                     /locus_tag="SCK51_001936"
     CDS             complement(5741..7861)
                     /locus_tag="SCK51_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG1252556.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8091..9269
                     /locus_tag="SCK51_001937"
     CDS             8091..9269
                     /locus_tag="SCK51_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEG1252557.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9285..10043
                     /gene="aroD"
                     /locus_tag="SCK51_001938"
     CDS             9285..10043
                     /gene="aroD"
                     /locus_tag="SCK51_001938"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEG1252558.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10066..10551
                     /gene="ybaK"
                     /locus_tag="SCK51_001939"
     CDS             10066..10551
                     /gene="ybaK"
                     /locus_tag="SCK51_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEG1252559.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10622..11875)
                     /locus_tag="SCK51_001940"
     CDS             complement(10622..11875)
                     /locus_tag="SCK51_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1252560.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            11993..13342
                     /gene="gdhA"
                     /locus_tag="SCK51_001941"
     CDS             11993..13342
                     /gene="gdhA"
                     /locus_tag="SCK51_001941"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEG1252561.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13454..14800
                     /locus_tag="SCK51_001942"
     CDS             13454..14800
                     /locus_tag="SCK51_001942"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEG1252562.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15108..15494)
                     /locus_tag="SCK51_001943"
     CDS             complement(15108..15494)
                     /locus_tag="SCK51_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1252563.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(15543..>15715)
                     /locus_tag="SCK51_001944"
     CDS             complement(15543..>15715)
                     /locus_tag="SCK51_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEG1252564.1"
                     /translation="VETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQR
                     RFEGTLRYSLGY"
BASE COUNT         5132 a         2813 c         3163 g         4607 t
ORIGIN      
        1 tgaaggaaca tgcttaaacc ataattgtgt ttttccttcc atcagcataa aagttcacct
       61 caaaaagagt gtacgaacaa aagttctttt tgtaaacctt aaaatagatt ttcctgaata
      121 cgtattgggg atttctaaat gtttgaatgt ttttttgaat gcaaaatcaa tgcaaaaaat
      181 tttgtatttc tctacttttc tctccttgac caaaataaaa aaacgttgat ttaacaacgt
      241 ttttcataca tgtttatctg tctcaaccat acatatatgg agacggcggg agtcgaaccc
      301 gcgtccaaac acatcgccac ttaaaaatct acgttcatag tcaactcatt taaggattca
      361 ctttacagct tgccgagtga caggcattct gtatcgctag tctgatgttc tctttttcaa
      421 cttacagacg gaaagttgaa acgtatccca ctaaagttag gacccttact cgagcacatg
      481 ggcgatgccg agaggatcta cgctaactgt ttttaggcag ctaaagcgta agagttgttt
      541 tcgtttttag cagttatatt taactgtaac gtttttacgt agacgtaacc tacgaaacgc
      601 aattcaagct cgactgtgcc tgtcgaatcc gtaacgtccc cgttagctaa caagggttac
      661 gtgtattttt catacaaaat tagtatagca tatattggga taaattcaat aaaaaagcga
      721 atcatatccc attatttata taataactgc ccctttctta agaaaaaata agcgattttc
      781 ttaagaaaag aggtagcttc aaaaaaagat cttaaatcag atcaaaatac ttcaatgcgt
      841 ggacaattcc gccttctgta tttttctttg tgataaaggt agaaagttct tttaatccgt
      901 cataggcgtt gcccatagcg attttgttgt cgcaagcttc caacaaggca aaatcattcg
      961 gaccatcgcc aaatccaaaa gtaggaactt tttccagctg aagttctttt tgcagacgtt
     1021 tgacacctgc cccttttgag attcctttct tcactgtatc gatcgagtaa ggaccattcc
     1081 gataaaaagt tagctcagga aaatgttcgt gatagtattc gtcacctgat tgagataaga
     1141 tcagcaacat atttactgtg ttttctttaa agcctaaagg atcaatttcc ggaacatctg
     1201 aatgaatgaa gtcgtaagca ttgatcattg tttggctatg accggtacac cagattttct
     1261 gatcgttata gtaagaaagt tcatgaccgt tcaactgtac atgatcatac attttttgac
     1321 attcttctga agaaaatgtt tgagaataga tttcttcgcc gtctactcga atgaaagaac
     1381 cattcatggt gaccgcagaa gtaatgcccg catcttccat gatagagtgg atttctgctt
     1441 ctgtacgtcc tgtagcgatg acggggagaa cattgttttc ttttaatgcg ttcatagctt
     1501 ttgccacttc gggtgtgatt tttgatttcg catctagtaa tgttccatca agatcaaaga
     1561 aagtgattgc acgataattt gacacaaata aaactccttt tttattggaa taagtttatc
     1621 ttaacaaaga atgaacaaaa gacaaacctt tcgtgaagaa ttcctgaaca tattggttca
     1681 gtcaacaatt tagagtatac tataagaaga gaatttagga gggaaagaat gatttttttc
     1741 agtattggta tcttattctt aataattggc agtctttttc ggattctgcc ctcaaaagga
     1801 aatctgcctt tttatgggta tcattcgcct cttgccgcaa aaacagacgc tcattggcgg
     1861 ctggctcaaa agacaagcgg aaactggttt tttcttatgg gcttactgat ggcattgatc
     1921 ggctattatt taaagacatc tgggcatacg aattattttc tgattgaaat gcttctgcta
     1981 gtatttccaa ttatgcctat ttttattatg acagaaaaga aattacagaa atatgacctg
     2041 gaaactggag ggaacgataa tgaatatttt aatgattgaa gataatcaat ctgtttctga
     2101 aatgatgcag atgtttttct taaatgaagg ttgggaagca actttcaagt atgatggaaa
     2161 agaagggctc gatgcctttt tagaaaaccc acaacattgg gatatgatta cactagacct
     2221 gaatttgccc acactggacg gtatgctagt aggaagagag atccgcaaag tttcaagtac
     2281 tgtgccgatt atcatgctga cagctagaga ttctgaaagc gatcaggtga tcggcttaga
     2341 gatgggggca gatgattatg tcacaaaacc tttcagtcct ctgacgctta ttgctcgaat
     2401 gaaagctttg catcgccgta gtgagttggc agaagcaaga gaagaagcag cagtttcaga
     2461 tgatcatttt gatatcgaga ctagtcattt caagatgaat acgaaaacac gagaagcgta
     2521 tctggacaac cggccaattg aaggactgac gcctaaagag ttcgatctat tgtttacttt
     2581 agcaaaaaaa ccacgtcaag tattctctcg ggagcagttg cttgaattag tctgggatta
     2641 tcagtacttt ggtgatgaac ggacagtgga cgcccatatc aagaaattac gacaaaaaat
     2701 cgaaaaggtc ggaccacaag tcatccaaac tgtttgggga gtcggctaca aattcgatga
     2761 ttctggtgtt gcctaatgcg ttatttgtat cagcaactct tagctttttg cggaatggtc
     2821 gcgttgatct tattgatcgt tggagtttct tttacacaac tgacaaaaca aacgattgaa
     2881 gaaaacaatt accagcaact gtttggatat gctgaatcag ttgaaaagac aacgcaaacc
     2941 tatgccgata cgctcccgca gtttagtcaa gatcaggcgt ttcaaaatgc attgctgctg
     3001 acagagcaag tactgacaga acaaaatgtg aactttatat ttatcgataa aaacgaacgt
     3061 gtcatttatc cgactaatac agctttactg aattttacct taacagctga tcaatggaaa
     3121 aatctgagga atggacgtca agaaaaattc acatcgaata aaaatatttt aggacaagca
     3181 caggcgacct cttacgcact tgtgccattt aatttgaatc atgaattcta tggagcactt
     3241 gtagttagtc aacctgcgcg aaatatcgat aacagcgttc gtccaattac actgaacctg
     3301 ttcaaaggat ttattttttc cagtatcgtt gcagttattg ccagctatgc ttttgcagca
     3361 ttccaagtta agcgtatcaa tcggttaagg aatgcgacga aagaagtgac gaatggtaat
     3421 tttgatgtac agctccccgt acatgacaaa gatgaatttg atgagttagc agatgatttc
     3481 aataaaatga cgaattcatt gaaagaatct caagcagaaa ttgaagaaca agaaaatcgt
     3541 cgaagacaat ttatggcaga tgcttctcac gagatgagaa cacctcttac gacaatcaac
     3601 ggacttctgg aagggttaga atacaatgca atccctgaaa atcagcgaga aaacgcgatt
     3661 aaattgatga aaaatgaaac agagcgtctg atccgtttgg tgaatgaaaa cttggattat
     3721 gaaaaaatcc gtacgaatca aatcagtatg gtcattaaaa aattcaatgg aacagaaaca
     3781 ttgaaaaata ttgttgcaca gttagaagct aaagcagaag cagcaggaga tacattgaca
     3841 ttgaaagccg ttgatgatat tgatgtttac gctgattatg accgatttgt ccaaatcatg
     3901 gtcaatatta ttcaaaacgc gatacagttt actgaaaacg gccaaatcac gattacttta
     3961 gaaaaaggct atcttgaaac gatcatcacg attgaagata cgggaattgg aatgtctgaa
     4021 caacaaatga aaagtatctg ggatcgttat tataaagtag atccttcaag aaaaaataca
     4081 aaatatggtg aatcaggatt aggtttacca atcgtgcagc aacttgtacg cttgcataaa
     4141 ggaaaattag aagtagaaag cgaactagga aaaggaacaa aattcgctgt tcgtttgcct
     4201 gacgtagaaa tcaaaaaaga gtaaaaaaca agcggtataa aagatcatga atcgatcttc
     4261 tataccgctt gtttttacta taaaatgatt gatttagccc gcgggctcta aacgatcaat
     4321 ataaggactt tgagcaatag tagcatcatg agtaaagagt aagatacctt gattttcttc
     4381 atgagctaaa taagaaagca agtggagtag ctcttggatc tccaatacac taagttcatc
     4441 aaatgtatct tcaataacaa tattgtttcg cttggaaagc aaggcatgaa gtaattgcag
     4501 ctttactttt tcaaaagacg tcaactcagc tgccgcacaa ttaagcaagg aaggagagat
     4561 atcaagtttt tcttgatagg ccaaacgatc ttgtttgatt tctttttccg gtacatccaa
     4621 caggacatgg tctttaagat tcagataagg aaacatcggc caattttttt ggatgatcgc
     4681 aaaattatcc agttttgcaa caagaacctc ttttttcaag tgctccaata ttttatgctg
     4741 tttttcgaga ggacacaaaa taacggtcaa gtgttcagca tgaatcaaat attcaggttt
     4801 cattacgtcc aggcctccta ctttgcaaat gatggataaa aaaagtgaaa gggaatagac
     4861 taagaatgag acagcatcca ctgagcccaa taagagtttg ggcaaatccc ttaagtatat
     4921 ctgtgaaaaa aagatcattt tggaaatcga tggtaaaaaa agtctgttga tccatcggaa
     4981 ttgtaagtcc gcttaccgaa gtcaagatat cacggatatc tggcagttca gtagtcttta
     5041 agacaagaaa attgatcctt tgaagaagcg cttcataaac tgactggaag cttacgatcc
     5101 acaataaaac caagatacaa caaagaaaga gaggaaagaa cagctgccag aaaataaaaa
     5161 ggaataattt ggttttagaa agaccagtca ggcgccaatt gatcaattcc tgcttgtttt
     5221 gcctgactga atagtaaaag aaaaagataa aaaggaaggc aaatagaaaa aataaactga
     5281 atatgccttt tatataaaat cggctagcta attctaaagc cggaagaagc tctgtagatg
     5341 cagaagcgcc aataaatgaa agccgatctt ggacttgatt ggctaaagcc gattgcaaat
     5401 ggtaaagagt caggcatgct atcaataaaa aagcagaaaa gcttgaaaat aaacaataga
     5461 gtattgtctg ctttttgtaa taaattaatc gagcagtagc atagcggatt attttcatac
     5521 ttttcacctc tataaaaagt atagaaaatg aatatgaata aagtatgaaa ttcctttagt
     5581 gaaatcacga attaaaacca gaaaatattt tgtaaaaagc aagaggttgt gaagaagctg
     5641 ccctattcta ttaccgtaga tgcaagcttt tgaacactgt ttattcgaaa agaggagcag
     5701 cgacaataat tttgtcgctg ctccttttaa atttattttg tcattcggaa gaacttgtgg
     5761 tcgttccgtg aagttcttgg tacgtttgcg ttttataaag atcaacagtt gattgtccgt
     5821 tgtgttcact aaagacacta gttgacttgt caccaagaga tttttcagct tcttttaatt
     5881 tctctaagcc attcttgtag tcataatcac tggtatcaat tgggtctaat ccactattcg
     5941 tatagaaccg aagaagatca ccattattga tttggtcaga aacagctaac tgtttatcaa
     6001 ctttttcttt cagtgaatca atttcattct gtacttcctc agtaggttca gtaatagctt
     6061 caccagtttc gttactatag atcgtaccac cgtagtaagt atattttggt gtaacaaaat
     6121 caccatcacg gaaagaaaca agctgttcat gatctttcga gaatagatct tgtcctaact
     6181 gaatataatt ttttgtatct acacctagta agtgaagtaa tgtaggcaag gcatcaactt
     6241 gtccgccgta agtgtgattg attccgccct gtgtttgtcc agggatatgg atcatataag
     6301 ggacacgctg catttgtgca ttgtcgtagt ttgtccaagt agaacttgtt tttccaacta
     6361 attcagctag atttttattt cttgaattcg agacaccgta atggtcaccg taaaggacga
     6421 tgactgaatt atcatacaag ccgctttctt tcaaataatt gaagaattct tgtacggcgg
     6481 tatctaaata attggctgtc gcaaagtaac cgttgatcgt ttcatcattt gtattggcta
     6541 acgggaatcc tgcttcgtca ttggtgaatt ttgaataagg ataatggtta gatacagcaa
     6601 tgaattttga ataaaatggt tgttgcagac gttccaagta ttgtacggat tgttggaaaa
     6661 atggtttatc atgcaacccg tattggaacg aattgtcatc attaacatca taataagaag
     6721 aatcaaagaa ataatcatat cctaaattct tgtatgtctc gtttcgattc cagaatgtac
     6781 cagcgttccc atggaatacc gcactagtgt aaccttgtgt ttgacttaaa atatcaggtg
     6841 cagcttggaa agtatttttt ccgcctaatt gtgtgaacaa ggagccttga ttcaagccaa
     6901 acaatgaatt ttccatcagc gtttccgcat cgcttgtttt tcctgctttt acttgatgga
     6961 aaaagttgtc aaaactgaac gtgctgttgc tgtggtaaag actattgatg aaaggtgtaa
     7021 cttcatgctg gagaccattc tcatctgtca atttataatc gatcaggaat tgctggaagc
     7081 tttctaaatg gatataaatc acattttttc ctttagcgag cccgaacaaa tcgtcatttg
     7141 gtgctgcata atgttctttt acataatttt ctacttcttt catatcattg ggacttgctt
     7201 ctgcccgcac ttggtttgtc tggtaagttt gaacaccgtc ataaaccatg aaagcattca
     7261 agccaagata tttgaccaaa taatcacgag agaatgttcg agtcagcaat tctggacgat
     7321 cagcttccgc cataaaaaga tttcctgaga aaatcataac tgctaatgta gagatcgcaa
     7381 aagccattct tgctcggatc gggcgagggt ccaacttgat tttttttgca atcagcagaa
     7441 cagtcaagat aataaaatcg atccaataga tcacatcata tgggcgaaac agtcgaatgg
     7501 cactttctcc cagaccactc gctactttcc cagcacctaa aatcgtgttg atcgtaatga
     7561 agtccgtgaa ttctcgataa taagtgacat tcgaaaaaac aagcaatgtc aaaagaaaat
     7621 aaatgaccat catcgtgata taagcaactc tagggcgtct gatataaaga gcaattccaa
     7681 gtaataacaa tgtagttgca atagggttga taaacaagat aaagtattga atgccacttt
     7741 cgattccgag atggaaatca actaaatagg caaaaatcgt tttcaaccaa agtaaaacga
     7801 ctagtaaaga aaaaaatccg agcctcttat tcaaaaaggc aggtgttttt atacatttca
     7861 agataatctt cctttctgca cttcttaatc acagaagtgt catgaaacca tgttacacag
     7921 ctcacttatt ctcatttatt ttactagtat gacaatatag tgtcaattga aaccagaagt
     7981 ttatgctgtt tcattaaggt tttctttaag cttcatgaaa aaatgagcag taagacaagt
     8041 cctatgtttt ttgttatact tgggaagaga aagctaaagc aggtgaagga atgaaagtaa
     8101 aagtaaaaaa aagagcaagc aatcgattga aaaaaggata tccactgatc caaaaagaag
     8161 accttgctca tccccaaaaa gaaaccacgc aatgggtgga attagttgat tcgcaaaacc
     8221 aatttatcgc aaaaggatat ctaggaaagc agaataaggg aatcggttgg gtattgagcc
     8281 agcagaacat cccatttgat caatcttttt ttgaagaatt ttttgtgaag tcaaaagaaa
     8341 aaagaacttt ctttttttca gatgattcta ctaccgcttt tcgtatattc aatggagaag
     8401 gagacggaat cggcggtctg attgttgatt attatgcagg ttatgctgtt ttttcttggt
     8461 ataacgaaac gttatatgca catcgtgaat tattgattca agcttttcgt gaaatctacc
     8521 cggaaatcgt aggaggctat gagaaaattc gatttgaatc agtaaatctg cctgagagcc
     8581 agttcattta cggaaaagag gcaccggaac ccttgatcgt aaaagaaaac ggagtaaaat
     8641 ttgccgttta tttaaatgaa ggattaatga ctgggatctt tttagaccaa aaagaagtac
     8701 ggggatcatt agcagaaggt ttcgcttctg ggaaaacagt tttgaacatg tttagttata
     8761 ccggcgcctt ttctgttgct gccgcaatgg gaggggcaat cgaaacaaca agtgtcgatc
     8821 tagcaaaacg cagtttaaaa aaaacacagg aacagtttga agtgaatggt ttggatgtcg
     8881 atcagcaaaa aatcattgtt atggacgtat tcgagtattt caaatacgcc aaaagaaaac
     8941 aatttttata tgacatgatt gtattggatc caccaagttt tgccagaaat aagaaaaaag
     9001 tctttcgcgt agcaaaagat tatggggaac tggtcaagga tagtctggat attttagcag
     9061 aagacgggat acttattgcg tctacaaatg ctgcgaatgt ctcgatagaa caattccaaa
     9121 caatgattga agaggaattc catgccaaaa atgtttcttt tgaacaactt gctttatatc
     9181 gtctgccaaa agatttccag acgacggata cctttttaga aggcaattat ctgaaagtgt
     9241 ttgtttatca aattaaaaat aaccattaga aggagcgatg cagaatggtt tgggtaaagg
     9301 gattgaatat aggaagcggt agaccaaaga tttgtgcgcc tcttatcggg gccgtccgag
     9361 aggaagtatt acgtgaagca gttcttgcac gtgaagcagg ggccgattta gtcgaatggc
     9421 gagtcgatca ttatcgtgaa gttttttctt ttgatgaagt agcagaaaca ttggttctta
     9481 tccatgagat gcttgatgga ctgccgttat tatttacgtt tcgtacctta gctgaaggcg
     9541 gagaacaaga catatcgatc cgtcagtatc gcgaccttta tgaacatgtg atacacacag
     9601 gattagtaga tatggtcgat atcgagttgt tcaaagtaga aagtctagga aaaagactga
     9661 tagaggaaat caagtcttta aacattcctt taattatcag tagtcatgat tttaaagaaa
     9721 caccagcaga ccctgtcttg ctttaccgat tgaatgttat ggaacatttt ggagccgata
     9781 tcggaaaact ggcagtaact cccaataatg aacgagatgt tttgcggttg atggagttga
     9841 ctagacgggc aaatgctttt gtctccatgc ctattattac gatgtccatg ggaaatcttg
     9901 gtaaaatttc tcgtctggct ggcggaacga cgggttccgt tatgacattt ggttccttaa
     9961 gcagcgattc tacttctgct ccaggacaat taccggtcaa agcattgaag caaattattc
    10021 aattattaaa tgaaccgttg tagaacaagt ttgaggtgat attttttggc aaagaaaaaa
    10081 atagtaaaaa cgaatgcaat ccgtatggtc gaacaaaaaa agattcccta tacagagcac
    10141 gaatatgagt gggacgaaag ccacctaagt gcgtcatcgg tcgctgaaca gcttccagaa
    10201 agccaatctc gtatatttaa aacactggtt gctgtgggca atgtgacggg gcctcttgtt
    10261 gctgtgatac cgggagaagc cgaattgaat ctaaaaaaat tagcaaaagt ttcagggaat
    10321 aaaaaagtag aaatgctcca tttaaaagat ttggaagcaa cgaccggata catacgtggt
    10381 ggctgctcgc ctataggtat gaaaaaacta tttcctactt atttagatca aattgcagaa
    10441 agctatgaac aaatcattgt ctcggctggc agaagagggc tacagatgga gttagcacct
    10501 caagatatct gtgcattaac gagcggacaa tttgcagata taaaacaata aatcatacgt
    10561 ataaaagaaa gaaggaggcc gggatgttgt ttgtccagtc tccttctttc ttttttatac
    10621 attatgctct ttgcccggca ttcattcctg ctacatgccc tgtagcgaaa gccgctgtga
    10681 tattaaagcc gccagtataa ccattgacat ccagtaattc tcccgcaaaa tacagaccat
    10741 caatgatttt actctccaat gttttaggat aaatttcctt tagagaaacc cctccgccag
    10801 ttacaaatga tttctcaata ggaaaagttt tgttgattga tagcttaaac tctttgcata
    10861 gttgaacaaa atcttgaatt tgtttttgag aggcttgttg tcctgtcaga tgatccattt
    10921 caagccgctc aagaaagaaa actaacaatc gctcaggcaa tataccatgc caagcattga
    10981 ccaagttctt tttggttgct ttactttttt ctgtcaatat atgaataagc tcttgtttgg
    11041 tttgtgtagg gaaacaatcc agagaaagtg tcactggttc tcccgttttc tctaattctt
    11101 tattgacaaa acttgaacaa cgcagtgcag ccggaccaga aataccgaaa tgggtgaaaa
    11161 gtaaatccat cgtatgttca gtcaatacac gacctttttt atttaaaacg cgtaaagtga
    11221 tgtcttgtaa agaaagccct tgaagtgttt tctcctgaat atacggttca tccgatatca
    11281 agggggactc tgtcgcataa agaggcgtca ccgtgtgacc cactcgcttc acgatcttgt
    11341 acccatctcc agtagaacca gtagatggat aagtccgtcc accagttgtt aagataatgg
    11401 caggagcatg gaactcttcg aattccgtgc gtataccgta aattttatgt tctttgtgga
    11461 tcaatttctc tactcgagta gagaatagaa gcgtcacatc tagttctttc agacgattga
    11521 gcaatgcttc gataatcgtt tttgatttat ttgttacagg aaacatccgt ccatgatcct
    11581 cttcttttaa atgtacgcct tgtgattcaa agaagttcat gatatcgaaa ttattccatt
    11641 gcgcaaacgt actatataaa aactttccat ttccaggaat atgtgcaatc aaatcatcaa
    11701 caggacgatt gttcgtaaca ttgcaccgac caccacctgt catcagtagt ttttttcctg
    11761 cttttttgtt tttttcgatc aagagtacgc gtgcgccttg ttcagctgca ctgatcgctg
    11821 ccatcatccc gcttgttcca gcgccgacta cgatcacatc atacagtttt tccattccct
    11881 cacctcaagg aaagtgtagc atatctgaag aaagaaaaca gaaaaaaaat gttttgagcc
    11941 aaaagagaaa atgagctaaa atgagggaag ataagcaaag gagcgtggaa cgatgacaaa
    12001 cgcaacagaa tatgttcaag caattcaaga aaagctacat caaaaggatc aagggcaaga
    12061 agaattttta caagcaatcg atgaattcat gcccacggtt atgtcttttt tggacactca
    12121 tcccgaatat atcgaaaaga atatcttaga acttttaacg gaaccagaaa gagttattca
    12181 atttcgagtg ccttggcaag atgatgaagg aaactggcga gtaaatagag ggtaccgtat
    12241 ccaatacaac tcagctatcg gcccttataa aggtggattg cgcttccatc ctagtgtcaa
    12301 tctaagtatc ttgaaatttt tggcttttga acagattttc aagaatagtt taaccggtct
    12361 gccaatcggt ggaggaaaag gcggaagtga cttcgatcct aaaggaaaat ctgataatga
    12421 gatcatgcgt ttttgtcaaa gtttcatgct ggaattgtca aaacatatcg gcccatcgat
    12481 tgatgttcct gcgggagata tcggtgtagg agctagagaa atcggctatt tgttcggcga
    12541 atataaacga ttgaaaaaat atgatactgg tgtgttgaca ggtaaacctt tggatttttg
    12601 gggaagtaag atacgtacgg aagctactgg ttatggatta gtgtattatg tcaaacatct
    12661 attgaacgaa gaaaaagatt catttaatca aaaaacagtt tttgtttccg gaagcgggaa
    12721 tgtagccatt tatgcaatcg aaaaagtgca agagctaggc gggaaagttg ttacatgttc
    12781 agattctagc gggtacatct atgatccaga aggaatagat gtgaaactat tgaaagaagt
    12841 caaagaaatc aatagacaac gtttgacgga atacgcaaac caacgtcctt ctgctattta
    12901 tcatgctgga gaatctgttt ggacattgaa agaaaaagcg gatatcgctt tgccatgtgc
    12961 tactcaaaat gaaatagacg aaagcttggc cgaaatctta gtagaaaatg ggatcaaaat
    13021 cgtagcagaa ggcgctaata tgccatgtac agtaaaagct gtttctgttc ttcatgaagc
    13081 aggcgtgtgg tattgtccag gaaaagctgc taatgctgga ggagtagcag tatctgcttt
    13141 agaaatgagt cagaacgtcc agcgattgac ttgggaaaga gagcaagtgg atgagcagct
    13201 ggatcaaatc atggaaacga tttaccatac gtgtcgccaa acagcagccg agtacggcga
    13261 cgaaaaaaat ctgcttcttg gagcaaatgt tgctggattt gaaaaagttg ccaaagcaat
    13321 ggcagcagaa ggtttagttt gaaaaatgaa actttcttgg aaatattgtc agcattttgt
    13381 ttatttgctt gaaaaataat aaaaaaaaac gtaaagtatt acctgtagag taaacctata
    13441 aggaggagaa caaatggcac acattcattt tgactattca aaagtagcac cgtttgtcaa
    13501 cgaacaagag ttagggtaca tgcaaagcca agtaacagca gcccacaacg aattacggga
    13561 aggtactggc gcaggaagcg acttccgcgg ctggatcgat ttaccaacga actatgacaa
    13621 agaagaattt gcacgtatca aagaagcagc taaaaaaatc caatctgatt ctgaagtatt
    13681 agttgttatc ggtatcggtg gttcttattt aggcgctcgt gcagcgatcg acttcttgaa
    13741 tcacacattc ttcaatttat tagatagcaa cacaagaaaa gcaccacaaa tcttctttgc
    13801 cggaaattca atcagctcaa catatattgc tgacttgatc gaagtaatcg gtgatcgcga
    13861 cttttcagta aacgttattt caaaatctgg aacaactact gaaccagcga ttgcgttccg
    13921 tgtattcaaa gagcttcttg ttaagaaata tggacaagaa gaagcaaaca aacgtatcta
    13981 cgctacaaca gataaagcaa aaggtgcagt taaagtagaa gcagatgcag aaggttggga
    14041 aactttcgtg attcctgatg acatcggcgg acgtttctct gtattaactg ctgttggttt
    14101 gttaccaatc gctgttagtg gtgcagatat cgatgcattg atgcaaggtg ccgctgatgc
    14161 aagcaaagca tactcaagcg acaaactaga agaaaacgaa gcttaccaat acgcagcaat
    14221 gcgcaacatt ctttaccgta aaggcaaagt aactgagttg ttgatcaact atgaaccagg
    14281 aatgcaatac ttctcagaat ggtggaaaca attgtatggt gaatctgaag gaaaagacca
    14341 aaaaggcatc tatccttcaa gcgcaaactt ctcaactgat ttgcactctt taggacaatt
    14401 catccaagaa ggacgtcgca atatttttga aacagttgtg aaggttgaaa aacctcgtaa
    14461 atcaatcaca attcctgaac aagcagaaga tttagacggt ctaggctact tgcaaggaaa
    14521 agaagtggac ttcgtaaata caaaagcatt tgaaggaaca ttgcttgctc atacagacgg
    14581 tgacgtgcct aacttgctag tcaaaattcc agaaatggat gcttatacat tgggttatac
    14641 aatgtacttc tttgaaattg ctgtaggtat ttcaggttac ttgaatggaa tcaacccatt
    14701 cgaccaacca ggtgtagaag cttacaagaa aaacatgttc gcacttctag gcaaaccagg
    14761 ctttgaagat cttgctaaag aattgaacga acgcctgtaa gataagggtt tttcgctaga
    14821 tttaaataat cagtgataga aacatgttcg ctttaaaatg aaaactggta cccatttagg
    14881 tactcaaaca tactgcatcg tttattcaga ataaggggct gtgacaagac gtttgtcaca
    14941 gtcccttgtt tgtatagaaa tcgattgatt gccttgctct ttagtttgtt ttaggacttt
    15001 aagaacagtg aggctgactt cgaaggttaa ttctgttttt ttgttttcta gaatcagtaa
    15061 gcaaaaaagc actcacttcg agtgcttttt tgcgatatat tcttagctta agataggcct
    15121 aatttttctt ttacagatga gggtacctta tcgctggtaa ttactttttt ctctatgatc
    15181 cgcttttcgc ttgctttcaa ctgcaagatc gttccttcgg ggtaagcaga accattggca
    15241 gagtcaccag aagtaatgac atcgaaatct aactgtcttt tttcaccgtt ttcagcataa
    15301 gcatctacgt tgtatttaaa acctcttcca attacttccc cattatctgc tttgatatca
    15361 gtttctgcag gcattggagc tttgactacc gcataataat ctttacctac ataagtagac
    15421 tgatagtagt cccaaccttt ccatcctata aaagcaatca atatgataac gatcaaccct
    15481 aaaatttttt tcatgaaaga tctccttttt tatttttatt atacccgtgg tgctagttaa
    15541 ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc tcgacttaat
    15601 agaacactgt aagccagtaa tatgctttca aatctactct ctagcccttt aacagaacgt
    15661 gagttgaaat tttgacaacc taatttttct aaggaagaaa aaacagtctc aactg
//
LOCUS       DARBFO010000057        15643 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000057 DARBFO010000000
VERSION     DARBFO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.058"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(89..316)
                     /locus_tag="SCK51_001945"
     CDS             complement(89..316)
                     /locus_tag="SCK51_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1252565.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(419..3007)
                     /locus_tag="SCK51_001946"
     CDS             complement(419..3007)
                     /locus_tag="SCK51_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEG1252566.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(3027..3686)
                     /locus_tag="SCK51_001947"
     CDS             complement(3027..3686)
                     /locus_tag="SCK51_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1252567.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(3735..4238)
                     /gene="trmL"
                     /locus_tag="SCK51_001948"
     CDS             complement(3735..4238)
                     /gene="trmL"
                     /locus_tag="SCK51_001948"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEG1252568.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(4459..5325)
                     /locus_tag="SCK51_001949"
     CDS             complement(4459..5325)
                     /locus_tag="SCK51_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1252569.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            5434..6075
                     /locus_tag="SCK51_001950"
     CDS             5434..6075
                     /locus_tag="SCK51_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEG1252570.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(6137..7498)
                     /gene="mgtE"
                     /locus_tag="SCK51_001951"
     CDS             complement(6137..7498)
                     /gene="mgtE"
                     /locus_tag="SCK51_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEG1252571.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(7516..8415)
                     /locus_tag="SCK51_001952"
     CDS             complement(7516..8415)
                     /locus_tag="SCK51_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG1252572.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(8417..9214)
                     /locus_tag="SCK51_001953"
     CDS             complement(8417..9214)
                     /locus_tag="SCK51_001953"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEG1252573.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(9192..9896)
                     /locus_tag="SCK51_001954"
     CDS             complement(9192..9896)
                     /locus_tag="SCK51_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEG1252574.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            10027..10602
                     /locus_tag="SCK51_001955"
     CDS             10027..10602
                     /locus_tag="SCK51_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEG1252575.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            10720..11397
                     /locus_tag="SCK51_001956"
     CDS             10720..11397
                     /locus_tag="SCK51_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEG1252576.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(11476..13284)
                     /gene="pepF"
                     /locus_tag="SCK51_001957"
     CDS             complement(11476..13284)
                     /gene="pepF"
                     /locus_tag="SCK51_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEG1252577.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(13460..14458)
                     /locus_tag="SCK51_001958"
     CDS             complement(13460..14458)
                     /locus_tag="SCK51_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEG1252578.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(14684..15337)
                     /locus_tag="SCK51_001959"
     CDS             complement(14684..15337)
                     /locus_tag="SCK51_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEG1252579.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         4485 a         3376 c         2704 g         5078 t
ORIGIN      
        1 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
       61 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
      121 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
      181 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
      241 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
      301 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
      361 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
      421 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
      481 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
      541 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
      601 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
      661 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
      721 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
      781 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
      841 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
      901 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
      961 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
     1021 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
     1081 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
     1141 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
     1201 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
     1261 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
     1321 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
     1381 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
     1441 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
     1501 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
     1561 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
     1621 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
     1681 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
     1741 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
     1801 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
     1861 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
     1921 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
     1981 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
     2041 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
     2101 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
     2161 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
     2221 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
     2281 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
     2341 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
     2401 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
     2461 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
     2521 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
     2581 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
     2641 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
     2701 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
     2761 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
     2821 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
     2881 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
     2941 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
     3001 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
     3061 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
     3121 gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa ctcacttagc
     3181 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
     3241 agtgggccat ctttatgttg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
     3301 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
     3361 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
     3421 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
     3481 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
     3541 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
     3601 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
     3661 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
     3721 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
     3781 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
     3841 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
     3901 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
     3961 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
     4021 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
     4081 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
     4141 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
     4201 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
     4261 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
     4321 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
     4381 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
     4441 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
     4501 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
     4561 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
     4621 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
     4681 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
     4741 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
     4801 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
     4861 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
     4921 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
     4981 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
     5041 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
     5101 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
     5161 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
     5221 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
     5281 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
     5341 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
     5401 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
     5461 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
     5521 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
     5581 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
     5641 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
     5701 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
     5761 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
     5821 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
     5881 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
     5941 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
     6001 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
     6061 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
     6121 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
     6181 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
     6241 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
     6301 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
     6361 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
     6421 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
     6481 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
     6541 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
     6601 tcgacatacc caaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
     6661 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
     6721 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
     6781 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
     6841 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
     6901 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
     6961 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
     7021 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
     7081 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
     7141 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
     7201 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
     7261 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
     7321 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
     7381 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
     7441 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
     7501 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
     7561 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
     7621 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
     7681 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
     7741 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
     7801 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
     7861 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
     7921 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
     7981 ctttgcaaaa agcatcaaac cagatgtatc ccgatctagt cgcgtcacca catggattac
     8041 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
     8101 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
     8161 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
     8221 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
     8281 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
     8341 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
     8401 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
     8461 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
     8521 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
     8581 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
     8641 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
     8701 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
     8761 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
     8821 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
     8881 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
     8941 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
     9001 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
     9061 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
     9121 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
     9181 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
     9241 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
     9301 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
     9361 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
     9421 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
     9481 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
     9541 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
     9601 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
     9661 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
     9721 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
     9781 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
     9841 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
     9901 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
     9961 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    10021 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    10081 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    10141 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    10201 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    10261 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    10321 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    10381 accggaaagc ttacaacaaa acgggctgaa ttccctattg aagaaggact tttagctatt
    10441 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    10501 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    10561 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    10621 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    10681 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    10741 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    10801 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    10861 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    10921 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    10981 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    11041 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    11101 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    11161 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    11221 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    11281 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    11341 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaaa
    11401 aatacgtcga ctcccttaca agagagccga cgtatttttt tcgtttctat tcgagaaaat
    11461 caaaaataga aagccttata aacgatcgat caattcttct aattcattta gacgttgttc
    11521 aaacatcgac attgcatctt caatatatgc agcctgtgtc atatcaactc ctgctttttt
    11581 cattacttcc acaggataat cgctgttacc tgcttttagg taagccaagt agttctctag
    11641 tgcctctggt tcctggttta gtattttctt cgccagtgct gaagcagcag aaaagccagt
    11701 cgagtattgg aaaacatagt aattataata aaaatgcggt atccgtgacc attcgaattt
    11761 aatttctggg tcttcttcga ctgctggacc ataatatttt gcattcaatt taccataact
    11821 atcacttagg tattcactag tcaatggcac acctttttca tcttccgtat gcatgaaatg
    11881 ctcgaattca gcgaattgtg tctggcggaa aactgttcct ttaaacccat ccaagtaatg
    11941 gttgagtaca taagcccgta cacgagggtc tttttctgtt tccaataaat actccgttag
    12001 gatattttca ttcgttgtcg aagcaatttc tgctaaaaag atggagtagt cgccgtacac
    12061 ataaggctgg ttcgaacgag tgaaataact atgaacacta tgtcccattt cgtggacaag
    12121 cgtaaatagc tgatccagcg tatcatgcca attcaataaa atatatggat ttgtgtcata
    12181 gcttcccgaa gaataagcac cgcttcgttt ccctttattt tcgacaacat cgatccaacg
    12241 ttcagagaat gctttttcta cgatggccat gtattcttca cccattggtt tcagtgcttc
    12301 taaagctttt tcttttgctt cttcgtacgt aaaggtaatt ggagcttccc ctaagaccgg
    12361 tgtataaaga tcatacatgt gcagtttttc cacttctaat aaacgtttcc gtaattccat
    12421 gtatcgatgt aacaaaggca aatgcttgtt taccacgtct accaaagtat cgtatacact
    12481 ttcaggaata tgattattgc tcaaagacgc ttctctggca gagctgtaat tacggacttt
    12541 cgctttaaaa ttatgtcctt ttatatgtgt gcctaaagta gaagcaaatg tatttctaaa
    12601 ttgttcgtaa acactgtaca atcccttaaa tgctgcttcg cgcacccttc gatccgtgct
    12661 ttctagcaac tgaccataca cgccatgaga taattggact atttcaccat tttctccttc
    12721 aatcgttgga aaaactagat ctgcattatt caaaacagca aatgtatcac ttgaagcatc
    12781 aaagatttca cctgctccag caagaagaga ttcctgctca gcagataaga catgggctcg
    12841 attatctact atttgctgga tataatggcg atagacttcc aattttggtt cttctttgaa
    12901 atattgccaa atctggtcat ctgacaattg caatatttct ggttcaaacc aggaaacggc
    12961 ttcactaact ttcgaaaaca gactgcttgc tcttgcataa agcgcttggt aatctgtatt
    13021 tccagtgtct tgatcgtttt taaggtgcgc atatacataa atgacttcag tttggcgata
    13081 aaccctcaat acgaattcaa tcgcatttaa aaattgagaa gcgccttgat caagtgttcc
    13141 tttgtgtttt tcagattgtt ttaactcttc tgataattcc aaatatttct catcaaactc
    13201 ttggtcgctt gaaaagatct tggtcaagtc ccaagttaaa ttttcaggca attcttctcg
    13261 ttttggcaac tgctttactt ccatgaaatc cactcctttt cttcccttag ctctatctat
    13321 caaaatagat catagtctaa catccacatg tcacagcatc taacttttct actcattcat
    13381 gtgtctgctt ccatcaattt tagcataact aaatcttgtt ttacatagaa aaccgattcc
    13441 tagcctattt ttatacttac tattttttca aagaagacaa gtgtatgcat tcgttaaaaa
    13501 actgctgata gaccatctct tcatcaatca taggaaaaag ccattccctt ttatgttctt
    13561 ttatttggca aagccatgag ttgaaatcag gaactttctg gttttccaag gcttcttcgt
    13621 acaacattcg ataaagaaaa acctgttctt gtaaataaaa gaaaaaccga cttttttgat
    13681 agatccacga tgatagatgc aaaagatgtt tatgacgcaa gtagcattgt tcttgtatgg
    13741 atactgtatt cttttgcttg tgaataagtt ggcgtgtcag ccatttcttc ccatcttcac
    13801 tcactttttc tatttttttc atttggttca ttttaatttc gttactagtg aatatttctt
    13861 taaggtccct actagaaaaa gtccagcagc tactgcaaaa ggaaatatgc cctgatacag
    13921 tctctgtaat atgatgatac aaataaagct tttgtttctt gagatcagct ttccagcaat
    13981 gcactcctct ttttctatta tagtagcagc aacttttttc tatcaatgag aattgcgatt
    14041 gtccaagaaa agaattacca agtatccacc atacttggta actgttattc aaataatttt
    14101 gggttctttc aataaatctc tggtgagaca gtctagaaca ttggatttct attgctagat
    14161 tcgccaacaa aatatccggc ctttgccgca acctcggcaa ataggcttcc aaaaatactg
    14221 gttctactga ctgtcccagc caatccatga agcattcctt taactgtaag tgctcttttg
    14281 attctctttc actaaagacc gaacagttat ctgatttttg atgggcaaaa tgagcacatt
    14341 ttttctttcc ctttttcaaa atcactcttt ttcgacagcc acgacaataa tatttctttc
    14401 ctttttctgc atgtttagca aaacagcact tattatattc attcattgca agcagcattt
    14461 ttctccctct gaacttattt acgtctaaga atttatttac acgaaaaaag agggcgtgaa
    14521 aaagctattc tgcatcaagt aataagaaca gctaaacaaa aatagttccc aatatttttc
    14581 gtttggttgg gcttgttacc gcagatgcaa gcttttgaac acatttatac gaaaaaaaga
    14641 ctgcgacaca atttttgtca cagtctctta ctataatgct gatttataca aaataaaatt
    14701 tcgttcgctc gatagcatta cgcgccatca gacattgtcc atattcattc aatgtttctg
    14761 gtgtacgatc gctcttttca gcaaactcca aaattcgagc aatctgattc tctacatccg
    14821 cttggttgag attttccatc cagaagtgaa cgactaggta atagcgctct tcgaataaat
    14881 acaaatcagt ccaagcagac tctagttcga catctgttgc aagttggatc acttgttcaa
    14941 aatcttcaaa agcaaaagtt gcttgtcttt cctgttcttc tagggcgctt ccagataatt
    15001 cttcctccgg atcgctgcca ataatctgct gacgaagaaa gtcactcaca tcttctgaat
    15061 caacagtaga atcttctgat tgagaaattt cttcatccgg aagattttta ctgataaaca
    15121 gttctaatcc gtctcctttt ggcaaaactt ggaaagtgac agcttcgctt cctcggaact
    15181 cctcttcgat atcaacttct tccaaaatac tataaaagaa attttcgatt tctttgtgat
    15241 tgccaagtaa atccaaaaat gaaacgcccc gttcctctaa atcttcatga ccaattagta
    15301 cacggatcgt attttcatta atatgttcca tttccatttt gctacacctc actttgccac
    15361 aatgtttaat atagattcat tgtagcgaaa tcagcttaaa tgtaaagaaa aaaaatctcg
    15421 ctgttaagcg aatttaataa aaccagcgga tttttcaacc tttcaagcta tcttatttaa
    15481 ctataacatt tttggcagac gaatactata tagccgactt ttcagctaat tttccctctc
    15541 tatttttcgt gcaatcatca tcttctacta cctttttata catctaatca agaatacttt
    15601 ttgtaaaaac aaaaaaactt ggaaaaacta tctttgtaat agt
//
LOCUS       DARBFO010000058        15625 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000058 DARBFO010000000
VERSION     DARBFO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.059"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            212..562
                     /locus_tag="SCK51_001960"
     CDS             212..562
                     /locus_tag="SCK51_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEG1252580.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            661..1191
                     /locus_tag="SCK51_001961"
     CDS             661..1191
                     /locus_tag="SCK51_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEG1252581.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            1345..2496
                     /locus_tag="SCK51_001962"
     CDS             1345..2496
                     /locus_tag="SCK51_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEG1252582.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(2605..4644)
                     /locus_tag="SCK51_001963"
     CDS             complement(2605..4644)
                     /locus_tag="SCK51_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEG1252583.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4702..5259)
                     /locus_tag="SCK51_001964"
                     /pseudo
     CDS             complement(4702..5259)
                     /locus_tag="SCK51_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            complement(5798..6004)
                     /locus_tag="SCK51_001965"
     CDS             complement(5798..6004)
                     /locus_tag="SCK51_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252584.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(6020..6634)
                     /locus_tag="SCK51_001966"
     CDS             complement(6020..6634)
                     /locus_tag="SCK51_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEG1252585.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(6781..7464)
                     /locus_tag="SCK51_001967"
     CDS             complement(6781..7464)
                     /locus_tag="SCK51_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEG1252586.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(7573..8031)
                     /locus_tag="SCK51_001968"
     CDS             complement(7573..8031)
                     /locus_tag="SCK51_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEG1252587.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(8115..9878)
                     /locus_tag="SCK51_001969"
     CDS             complement(8115..9878)
                     /locus_tag="SCK51_001969"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEG1252588.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(10099..11559)
                     /locus_tag="SCK51_001970"
     CDS             complement(10099..11559)
                     /locus_tag="SCK51_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252589.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(11767..12693)
                     /gene="glsA"
                     /locus_tag="SCK51_001971"
     CDS             complement(11767..12693)
                     /gene="glsA"
                     /locus_tag="SCK51_001971"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEG1252590.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(12716..14143)
                     /locus_tag="SCK51_001972"
     CDS             complement(12716..14143)
                     /locus_tag="SCK51_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1252591.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(14174..15388)
                     /locus_tag="SCK51_001973"
     CDS             complement(14174..15388)
                     /locus_tag="SCK51_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEG1252592.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         4476 a         3114 c         2686 g         5349 t
ORIGIN      
        1 taggatgacg ttttggtcga ctaaatttta ccaaaagtga gtctcttttt gagttgaaac
       61 gaagaaaaag tgttagtaga aaatcgcctt cgattttcca ccaacacaaa atattataga
      121 acctttgttt ttttgaataa tcgctttttc ttctcttttt cttatatact ataagtagta
      181 aataatttga aaaaaagaaa ggtgactgat tatggcgcaa aatcgtattt ttacgacaag
      241 ttttgcaagc gtttatccta tgtatgtagc aaaagcggag agaaaagatc gtataaaaga
      301 ggaagtagat gaaatcattt gttggctgac gggttattcc caaacagaac tagaatatca
      361 actacaaaaa caagtgactt tcgaacaatt ttttgcggaa gcccctgtcc ccaatccttc
      421 acgttccttg attacaggtg ttgtttgtgg tgtacgagta gaagacatcg aggataactt
      481 gatgaaagaa attcgttatt tagataaaat gatcgatgag ctagcaaaag gcaaaaaaat
      541 ggaaaagatc ctcagaagtt gatttttcca gagatattct tctccttcgc tcattttgta
      601 acatttaatt tggtaaaatg actagttcac gttaaaataa gtcaaaggag gttttcttat
      661 atgtttgaga cgattaaaca aaatattcaa aaatacgcct tattaagagc tgctgtttat
      721 attatttctg ggattgcgat tgtgctaaac ccttctgctg tttttcattt tatcggttat
      781 ctgatcaccg cctatcttgt tttgcttggg ttgttgaatc tttttgaggc ttaccgtttt
      841 aaaaggaaaa caggttcttg gagtatcggt ctgttcagtg gtcttttttt cctgatctta
      901 gctctgatcg tctatgcttt tgcacctgca atcgtttcta tcctgccgat tcttcttgga
      961 ttagctgtta tcatcaacag cttgttccag ttgttcttca gcatgaatac aaaagctaaa
     1021 agttggagtg tttatagtag tattctgttg ttgattggcg gattcgtctt attgttcaat
     1081 ccattcaaaa gtttgatgct tctattccaa atattcggct ttattttgat tttcatgggc
     1141 atccaggaga ttatcaattt catcagaata agaaaaatga ctatacgtta atcattttta
     1201 gttattaaaa tagaccgtat aaaaaccaac aatccaaaaa aatcttttgg attgttggtt
     1261 tttttgtgag tatattttcc tattttttca ataatgtatg ttatgctaag ttcgggctat
     1321 attttataca aaggaatgat gagaatggaa tttattggta ttttatgcct gatattgatt
     1381 tcaacaacga ttggctcaca tatctcgcgg agattaggta tcccagcagt gattggacaa
     1441 ttacttgttg gtgttttgct aggccaagct ggattgaatt gggtcaaacc tgatattcta
     1501 gtccatgact tttcggagat tggtgtgatc ttattaatgt ttttagctgg tctggaaagt
     1561 gatttatctc ttttgaaaaa atatttccga cctggtatgt tcgttgcttt gcttgggatc
     1621 atcttcccgg ttgtttttgg ctggttgaca ggcaaagcat tccaagtttc gggaaatgaa
     1681 gcgattttct ttgggattat tcttgcggca acttctgtca gtatttcagt agaagtatta
     1741 aaagaactaa acgtagtgaa tacaaaagaa ggatcaacaa ttttgggtgc ttctgtggta
     1801 gatgacattt tagtcgtttt agttctaagt ttcagcctat cttttctaac gggggaagct
     1861 tctagcaatg cttctttacc gcttgttttg attgaacagc taatttattt tgtttttatt
     1921 ttcttattag tcaaatggat tgcgcctttc ctactttcat ttgcagaaaa agtgtacgca
     1981 aattcttcta ttatcatcgt atcgctagtt atttgtttag gaatgtctta cgtagcggat
     2041 cttgttgggc ttagctctgt cattggtgct ttttttgctg ggatagctgt cagtcagaca
     2101 aaggttaaag aagaagtata ccacaatgta gaggctttag gatatgcagt atttatacca
     2161 gtattttttg tcagtgtagg attagaagtt gatttttctg actttagtga acaactactc
     2221 tttatttcta tattgacgct tgtagcgatt ttgaccaaat tgcttggagg ttattttggt
     2281 gctcagattg ccggtttttc tcaacatagt tcattgatgg tcggtgctgg tatgatttcg
     2341 cgtggagaaa tggctttgat cattttacaa attggacagc aaagtcaatt gatcgaacca
     2401 cagtactatt cacctttagt tatcgtgatt ttattaagta cacttatttc tccacttgtt
     2461 ctcaaatatt ttacaaaaaa agtttatgaa gtgtgagtaa atcaaaaaga agttgtgaaa
     2521 gagacatcgt gaaaaccgtt tatttgaaaa aaggactgtg acgataacgc cacagtcctt
     2581 ttactgtact taaaaagcaa ttttttattg ataattttgg ttgaggtatt gcaatagttc
     2641 cgatgctcgt ttggttgctg aatcatcatc ttctttattt tccaacatgc tgaccatcat
     2701 ataaccttgg ttatcaagat tgaaagcaaa caagaaactg ttctcttgcc cctttacatc
     2761 ctgtttttct ttgatttccg ctgtaccagt tttcgctgcc aacggaatcc ctaaagcaga
     2821 aagagaatgt gctgtaccat ttacatcttg cacaacttct ctcagatctg gcacgatcgt
     2881 ttgtactgct gtttcgctga tgacattctt cttatctttt gtctctttat ctgcaatcaa
     2941 tttaggatag acaagtgtgc cattgttggt aaaaacagaa tacattgctg cttgctggat
     3001 aggagtaatt agaagttcgc cctgtccata tccagtatca gctagcaaga tatctgagtt
     3061 aaagctatct tcattagaaa tttgtgctgg attcatacta attggcaaat caaggtcttc
     3121 accaaaaatg aatttatcta agcctgtacg aaattttttc tcacccattt tcaacgtttc
     3181 ttgtgccgca tatatattat cggaatagat caaagcagtt tttaagtcta cttgtgatac
     3241 atcactactg acacgcgttg ttttaaaaga tccccaagaa ctgtcttttt gccatttaag
     3301 cccgttgatc gtcaacactt catttggatc gatagtgccg ttgtcgagac cgattgctgc
     3361 tgtaatcatt ttaaacgtag atccaggagc ataacctgtc gcaaatcggc tgatgaatgg
     3421 atgttcagga ttttcttcat aagctttgta atcttcttgt gagatcccgt ttgtcatttt
     3481 gtttggatca tagcttggag agctagcaag cgcaagaaga tcaccggttt ttggcgttgt
     3541 cgcaacagtt gatgcagctt ttcctcctag actgtcaaaa gctatttttt gtgccttcgc
     3601 atcgattgtc aatttgatat ctttgccatt ttgaacttca tgttcgatca gaactttttt
     3661 ctctacaccg tctgcatctg taatgcttaa tttcccacct gttgtcccac gaagatcctt
     3721 atcaaaagcc atctccaaac ctgagcgacc gatttttcca ttactgctta attctggatt
     3781 cttatcaata tcttctgctg tgatatctcc cacgtaacca atcagctgag cagctgcttc
     3841 acccaaagga taatatctgc cgtctacttc ttgaatggta gctccagctg gaagttctgg
     3901 cgttgctcca tcaatgattt tcaatgggac aaagtaatcg ggttgtaccc agctttgtga
     3961 aatcgcctga ttgatagcat cttctgttaa gtcgaaggag gaagcaatcg ctttgatatt
     4021 ggctgttttt tcgcctccat ctccaagttt acttggaacg actcctaatt gttttagttt
     4081 gcctgttgtt gctaatggtt caccatttcg atctataata ttccctcttg ctgcttcttg
     4141 tgtggtcagg cttactttgt catttccttc catttctgga aaaaccaagt tgggttgcca
     4201 gttgatcgtg gtttgaccat catttctgtc taatgttcct ttataagaaa gatctttcaa
     4261 ttcacctaat gaggtattca tctttgcttt atatgaaaaa ctgtaagtag aatcgtcttt
     4321 tttatctact tttagattcg atatctgaag tcctttgaca tcagcagcac cgtatatatt
     4381 ctggtattta tctaagatct ctttttcaga taatgcactt ttatttgccg ccttttttga
     4441 tgtcatttct gcagctttgt tataatctcc cttgttcaaa gcttggacaa attgctcaac
     4501 tgtcttttct ccagcttcta ctgcttgggt ttcttgatag tgctggtagt aaaaataacc
     4561 gccacccgca gctgctatta agatcactgc gccggcaata taagcgcctg ttcgattttt
     4621 gccgtgcttg tcacttcttt tcattttttc ctccttctct tgcttgtcat tcctttattt
     4681 cactatttat acctgtttat tctaacatat gtctttgagg aaatccatac cacaatccag
     4741 aagcacactt tctggaaggt tcgtttggat actaccaagt aatttcccaa ccgctttggg
     4801 tagcttgaat attgaagtta cgtctttcag ctgctgagag atagctttga tcacttgctg
     4861 ctgccttctg atcctcccga agtctccttc tttgtcttcc cgaaatcgag catactggag
     4921 taaagtatta ccatccatca cttgctctcc tttgtttata tatacgccat ctaaatctag
     4981 agatttttct gcatcgatct tgactccacc aggaaacagt tcattaacgc aatcgataaa
     5041 atcttgaaat gtgatacttg cataataggg agcttcaaat ttgaaatttt cctttaatgt
     5101 ttgatttaat aaatcaactc ccccataaga gtaagctgca tttatcttat tcattccgta
     5161 acctggaata tcgacatatg aatctctcat gatagagatc agttttgctt gttgtgtttt
     5221 ctggttgtat tgtgcaacca gaagtgaatc cgagcgacct ccaccaatcg atcttttttc
     5281 accgcatctc ctcctcacac agcaatccac ttgtcaggtg aggagcgagt ttgtctcatc
     5341 aaataagtca gtcacaaaaa tataaagaaa aggtcagaaa ccttttcgaa caagaattct
     5401 gacctagcat catctcatcg tccgagtttt agcactatac aacgtaaaga aaacattctt
     5461 agctattgga aaaagcctta atccaacagt ttctgtttta cccattggcg actttcgacg
     5521 tttctgggca acagcctatg tttgtatagg agcctcacct aacaaggtta ttactatatt
     5581 ctatttacga gttcatgata gacttttcct aatgattagg caatggaatt tgaaatgaaa
     5641 tagtgaaatt taacaatctt taatattttt tagaaaaaaa taacaaataa gttgaaagaa
     5701 tcccgttgca aattctttca gttgcaccag gattctttga taatttttta gttttatgtg
     5761 tttttttata tgttttttca cttttcggaa taaatattta tttgttaaag taattaaaca
     5821 atccgctaat cgtttgatct ggcatttcta tttccttacc atctaaagta acagtagtaa
     5881 cgacaaaatg gtcttgcaat ggcggaactt ctaagtactg cccttgcaga aaatcattca
     5941 ctgtttcata ttcttttttc atttcttttg aatctgtcga tggtttatag cttattttta
     6001 acataactag tgctcctttt tattcgggac gtgcaatcgt gaaaatttct cctattgctg
     6061 cataatcatt tcctgccaaa cggcttacag cattcaattt tcttggatcg atccttcctg
     6121 tcttatcatc atagactgtt tcatttaaat gatatttctg tacttctagc aaaaataaat
     6181 cagctgttac tttattttta ttccgtatct cgacatgttc atataaggat gtttcaaaac
     6241 gaacttttgc ttctgtcaat gctggtactg aaatgattac tgaatcagtt aaagtcaagt
     6301 cagtcaggta cagttcgctt tcctcattag gcagattggc tgctgtttta tttacttctt
     6361 ctacgttgtc ctcatcaagg atatggataa cagcttctct tgtctcaaga atattctttg
     6421 ctgtgtcttt tactgcatct tgttttctct ggatcgccaa ggataagatg ggtcgattcg
     6481 aactgacgat attgaagtaa ctgaagggtg cgatattcac tatatctttt gctgatttag
     6541 tcgttactac tgctactggt ctagggatga ctgaaccaat cagcaacttg taattttcac
     6601 gttctgtcaa ttctgaagga ctccgttcaa acatggtttc gccttctttc tataaaattc
     6661 gttaaaaagc agatcttgaa gacaaaatga aaagtaatgc ttttttcatc ttcttagaat
     6721 ctgctttaaa ctatctatca atttttcttt tttgtcaaga atctgtgccg tgcgtgtttt
     6781 ttagttattt gctttacttg cggcttcgac atatctttta gtgatcaatt gcggacgggt
     6841 gataatagat ccaactacga ctgaaaaagc acctaattca tatgctttct ctacttgttc
     6901 aggagagtca gtatgtcctt ccataattac tgggatatcg acttgttcta tgatttgatt
     6961 caataaatca aaatcaggtg tctgcgtctt tcgtgaatat tctgtatagc ctgctaaagt
     7021 cgtagacaaa agatcaaaac catcttcttg cgcttgaagc gcttcctcaa aagtcgaaat
     7081 atctgccata gctaaccgac cgtgttcgtg aatcatttgg acaagttcag cagttgtttt
     7141 ttctgtcgga cgtttacgag aagttgcatc cagtgcaatg atttctgctt gcgtctccaa
     7201 aagttcttcc acttcttttt ctgtcgcagt aataaacact tctgacccat cgtaatctct
     7261 tttgataatc cctattaccg gtaaatcaac gattcgcttg atttcattga tatcttctgt
     7321 cgtatttgct cgaataccaa ctgctccgcc ttcttttgca gccaaagcca ttctccccat
     7381 aataaaagaa ctgtgtagcg gctcgttttc caaagcttga caagaaacga cgactccgcc
     7441 tttgatttgt tccagtgtat ccacaatatc tcctccagta atgttatata aaaatacagc
     7501 ttcatataaa aatgtaaccc ttttctattc aggagacaat aatgagctat ttctttcagc
     7561 agacctttct tactaaaaga aaattacttc aaattcttcg tatacgacca gcttctcttc
     7621 tgtaaattct atgttcaacg cttctttata agcatgttta aaaaactcag catggacttt
     7681 ccgccaataa gctaatgtcc gatccccttc tccttctttc caagcatgct ctttcgtgac
     7741 ttcactaaaa ggcaagatgg tcacatttat atttttgatg atacaaactg gttcattttt
     7801 tccattcagg accacgtcta attgaccaac ttttggtaaa ggctcattct cttgtttata
     7861 cagatcgtaa ccacttgatg taccagtctt tatctttttt acaactagtt cgctcaactc
     7921 gtctgccatt ttttctgtat ccccaaatga aaagaaacct aatttttctg attgataact
     7981 aggatatttt tcttggaact ttttgacata ttcttctact tgctgataca taacatccac
     8041 ccctttgctt tttataatga aaaaaccatc cagatactag tataaacaat tatctggatg
     8101 gtgagcataa tgaattattg aatattcaag acacgcagac tatcttcaac gatttgtttt
     8161 ggtgttaaat gataacgttc ataaagctgc cctacaggaa cgctatccgt gaattctttc
     8221 tctgcaccaa aatttaatac gttcattgaa gaatcaccat aataacgact gatattttcg
     8281 ccaaatcctc ctgacaaact accatcttct aatgtgatga ctaattcgtg tgtttctttt
     8341 aatttatcta atgtttctac gtcattttct gtgatcgttc ctggattgat caacgttgct
     8401 tcgattcctt tttttctaaa agcttttact gtttcttttc ctaattcaag aaaaccgcct
     8461 aaagctaaaa ttgctacttt tcctccatta tttactgttt gataggctgg aatactataa
     8521 tcatttgcga tagctgtacc atgttctaca ccatgtgtag gaattcggat tgctacaggc
     8581 tgcgctgttt gttcgaacgc ccaatctagc atagagatca tatcctcttt tgttgctgga
     8641 gcaagatatt tgatgttcgg gatacttgat aagtaaggaa tgtcaaatgt tccttggtgt
     8701 gttcggtcac tagctgagat cgttccgcca ccgactaaaa taatcactgg attctcgttg
     8761 atcgctaaat catgtgataa ttgatcatat gcccgttgca agaaagtact tgattggaag
     8821 acgactggac gtactccctg agaagccaaa gcagaagcga aagtgataga gtgctgttca
     8881 gcgatcccca cgtcaaaata atgatctgga tgtttttctt ggaatgtttt cagtccaaag
     8941 gcgcctggta tcgctgcatt gactgcggca ataggagctg tacttgtttg cattttctta
     9001 tccaaataat ccatgatgat catattaaat gtttctccag aagttacttg ctttgcctcc
     9061 ccggtggaca gatcaaatgg tacatgccaa tgatatttca ttttattatc gattgccggc
     9121 tgataaccgc gacctttttc cgtatggacg tgaagtacaa tcggatgatc aatatctttt
     9181 acttcttcaa ataaacggat caccgtttcc agatcgtttc cattatccac gtaacgataa
     9241 tcaaatccca ttgctttaaa taggttattg ctgcttttac cgttggtttg acgaagctca
     9301 gctaagtttt tatataaact gccatgattc tcatcaatag acatctcatt atcgtttact
     9361 aatacgatta gattggattg taaaacacca ccattgttca atccttcaaa agctaagcca
     9421 ccacttaggg agccgtcacc gataaatgct accacatttc ctgattcttt cttcagatct
     9481 ctcgctttag ctaaacctgt tgctaagctg atagatgtag atgtgtgccc cactgtaaaa
     9541 tagtcatgtt tactttcatg ttggttggaa tatccgctca cttcacggta atgtcctttt
     9601 tcaaaaccat tttttcgacc agttaaaagc ttatgaccat aagactgatg ggaaacatcc
     9661 caaacgattt tatcctttgg tgaatcaaat acataatgaa acgcaatcgt taattctact
     9721 acacctagat tcggaccaac gtgcccacct acatcgctgt ccttttgcaa gattttttgg
     9781 cgcatctctt ctgccaactg tttcatttcc tcaacagata attttttgac atctgcgggg
     9841 ctattgattg catccaatac atgtaatact gtactcataa tacattctct cctccgtact
     9901 atcttctttt aatgaaaata gatttgttac actttatcta ttctactact tagagttgac
     9961 tctaagtcaa tagcatttta aaaaaatttc agagaaagct tgttatataa gtagatcttc
    10021 gcacgaatca ggactcaaat aaaaaccttc tacagacaat taaaaagctt tgtagaaggt
    10081 ctcctaatta aggtctcatc aagaaatatg tttttttaat aggtgaagtt cctgagcaat
    10141 atgatacaaa tggatgtttg caccttttaa tgagattgga tagatctttg tcttttcagt
    10201 ttctaatcca ttttgatagc tagcagcatc gcacaaaacc actctttttt tcgcacatag
    10261 acattcatca ggcaattctt ctaatgaacg aaataaaata ggatttattt cagcgatgcc
    10321 ttcttccgca tacaaatgaa attgggttcg ataaaaaaat tgtttgactg aactttctga
    10381 aacaacaaaa atttctattt cctgtctccc cgcttgttca ctgttgatca aagaaagact
    10441 tttgacgaat tttcgaatga aattttcatt ccatcttagt tggatttgat ttttcttttt
    10501 ccactcttcc aatatttcta caatttttcc gtacagaagt tgttcttttt ctgatagaag
    10561 atagatcctt tccggtaaaa atacttgtcc atctgcccac aacgtacgga caaattgtaa
    10621 aaaggattgc ttgaaaataa aattttctgc atcgtcgatt tcagcgatac taataatctg
    10681 taaaatcaat tcattcagtg ataggtcatt gagaacaaaa tttttataaa caacttcgaa
    10741 atcttttctt aacagctcca tgttctcatt ggtataattt ctactgttga acaaagaaaa
    10801 aatataatag acatcttctt cttgtaagtc tagaattcct ttttgctctt ccaatccttg
    10861 acagactaat tgaaataacg gcttcgtttc tgcgcttttc ttatcttctt ctttcagtcc
    10921 aaggggaaat gcctttcttc cgattgcgat ctctattcct cgtatgataa aggacttttc
    10981 atcttccgaa tatcttcttt taaaaaaatg agtctctaca taatcaatca actcgttaca
    11041 agcttcttct acatacggat ttttattttc atagccttcc caattgacat aacgtactaa
    11101 agctagcaag atgttccgcg agtcaaattc tgggataatg atttctttat ttttcaaata
    11161 atcattttct ttaaaaaaat ccaatacgat ttttttgatt gtatatactt tgctcaaaga
    11221 aataaattca agatcagcca gttctgtact cgtgaatata ccattaaaat aataacttaa
    11281 aaactttaaa aaataagatt gaccgtacag aaactgtgtg agctcagaaa gagcaatacc
    11341 atgtttgaat acgagtttat acttatgctt ctctcttact tgttctttta tacaatattc
    11401 tagacactcg gctagacttt ctagatcgtg aaggatcgtt ggtgtagtca cccttagaga
    11461 aatagctgtt tgttcgatgt ctatttcttg gaattcaaat aattgttcac acagatacac
    11521 ttttctaaat atatttcgct cgatgtaatt ttccagcatt cctgatacac gcctccagtt
    11581 tttcttatga ttttatttaa tcatatctgt aaatcgtatt tttgtttcct ttttatttag
    11641 aaaagccctt tcaaaaacca ataaaaaaag aaactgggac aaaagtcaaa caagctcaca
    11701 atttcagcct cattgtaggt agctttgagc cgtttggact tttaccccag cctctttcaa
    11761 gaatggttat tcaaagatgt cagcgtctag ttctttgact acatctttca acaattggat
    11821 accagctgcg ctgttaccaa atggatctag tgccgggctg aagacaccta tgccgtaacg
    11881 atttggcaca gctgccatca acccgccgcc tacaccgctt tttgcaggta caccaacatg
    11941 gacggaaaac tctcctgatt catcatacaa accagcagtt gtcataattg atttgacgat
    12001 tgtagcactt tcttcagtaa tgatttgttt gccattccaa ggagcaattc ctttatttgc
    12061 cagcacagca gctaattttg ctatgcctgt agcggtgaca ttgagggagc attgtctgaa
    12121 ataagcatcg agtatctccg gtacgtctcc ttcgatcatt tgattacctt tcatatagta
    12181 agcaagagag cgattgatat cacctgttct tgattcagaa tgatagattt cttcattcaa
    12241 tgtgatatta ggatcatcac agatctcttt cataaatgac aagattcgct caaatttttc
    12301 atctgcatct ttaccggcaa tcaacgaggt tgttgcaatc gctcctgcat taacaaaagg
    12361 attcattgga tgtttgcgat gattgatttc catattcatg atagaattga aggcaaaacc
    12421 ggttggttcc gtgttgattg tttgaaagac tttttctatt ccgttgtctt tgattgctaa
    12481 taacaatacg ggaacttttg acatgctttc gatggcaaag cgcacatcgg cgtctcctcc
    12541 ctcaatcagt tgttttgtat ccaaatcata aatcgccata cctaattgtt tgggatccac
    12601 ttcagctaat gctggaatgt aatccgccac ttgtcctgtt tgataatagt ggaaattttt
    12661 tccaatcaat tgttctaatt cttttacttc catttgaata accagctttc attttttatt
    12721 tttctgcatg ctggaattta tgacggcatg cgtagatcgt aaatggaata actaagacga
    12781 taagataaga aacaatcaga atcgtttcat aaatcccatg ctgtccagct gcgatactag
    12841 caggtggaac aaatgagata caaaaagcaa aaagtgaagt caaaagacct aaacaagcaa
    12901 cgattacttt tacaactttt ccgcctggaa cctgataggc tctttcaaga ctgtcatctt
    12961 taaaaaccag cttcaaatag cccgcgaaaa acataaagta caacatcagg tagattacaa
    13021 cagtcaatga catcgctgca agaaatgaaa gattattccc cccgccgctt aatgtaaaga
    13081 cggctgccca gattgttgca acgatcccat ttgcgatcat caaatgagca ggaacaccgt
    13141 gtgtgttttc ttttgcaaaa tattcaggaa gtaatccttc ttctgctgct acgtgaagtg
    13201 cttttgaagg agaggttacc caagaagcaa tttctccgat gacacctaaa caaacaagaa
    13261 tcgcaagaat tttcgcaatc catgcaagat ttggatcaat gtgtcttaat aggaattcta
    13321 acgattgaat cacccctgta ttaagagaca aaccatgttg aggtactgta gtagcgacgg
    13381 tcaatccgcc aaaagtatct aaaatgattg ccagaataac caatacaaac attgctaatg
    13441 gataatcctt tttagggttt tccatttcat ttgcatgact ggcagatgtt tctacgccca
    13501 tatatgctaa aataaaggaa acaaaaacaa ccagcgtagg caatttcgaa aaatcaggaa
    13561 tcaatgtttt catagacagt atcgtttgta gtggagcgcc tgaagcgaca taatggatcc
    13621 ctaagccaaa caagactaat gctggcaaca agataccagc tacaaaaccg atccgagcaa
    13681 tcaatgaagt gtatttggtt ccttttaact gtgagagtaa aatagcccag aaaataatca
    13741 ataggattgc caatttcaga tatttattgt tttgtatagc accaataccc gttgcatagg
    13801 ataatgcacc agttaagaaa tacagcatgg tcaagtagcc aactgttatc tcaaaccatt
    13861 ggaaaaagat tgcggcaaaa ccgaatcgtt ttcccaatgt gcgactgacc caagtataga
    13921 caccgccttt ttcccatcct ttcaccgttg ccatctctgc tgcgcacaat gccacaggga
    13981 taaaccatag caaaccgcct aataacaaga aaaatactaa gctgaagcct gaggtcgcga
    14041 atgtgggata ttcatataat gaaacgacca ttgagatggt catagccaaa aagccaaaga
    14101 acgttaattt ttttgcagga attttcgttt tgctttgttg catgaatctt cctcttttct
    14161 atttgaattt gtttcaattg agtttgttgc caaagaagaa aaagtccaat ccttcttctt
    14221 gttcttcttt tgtagtagtg atcgttgtaa agtgcatcgc cgattttaaa atcactgttg
    14281 tcagcacata ggcatacacg gcagtaatga tgacaccaat cacttgaatc cctaagaagt
    14341 accagctgct tggattcgtt agtaaatggc tgttagatgc aaagatccct atcagaatcg
    14401 tacctgtgaa tccgcccatg ccatgaacac cccacacatc aagtgcatca tcccattgtt
    14461 tctttttacg aaaatcaaca gcagcatgac agacgatacc cgcaatgatc ccaatcagca
    14521 tggcactttt tgcttctaca taaccggcac aaggcgtaat ggctgccaat ccagcaacgg
    14581 atcctgttag tacgtcgaca aaatcaaagc catttccttt tacttttgca atgatcagcc
    14641 aagtaatcat cgcaaatgca agagcgatga atgttgaacc aaatgcattg actgcttgtt
    14701 catctgctcg aagtgcacca ccggaattaa aaccgaacca gccaaaccaa agcagtcctg
    14761 taccgatagc agctgccata agactgctat gctgacttct ttctcctaaa ggaatctttc
    14821 tttgaccaag tgtgaggata catgctaaag agccgaatcc agctgtcgtg tggatgactg
    14881 ctccaccagc gaaatctcta aatcccatct gatccaaaaa tccgccaccc catatccagt
    14941 ggcaaacagg gaaataaatc agcagattcc aaaaaataac aagtataata tagcctttta
    15001 aattcaatct ccctgcaaaa gctcctgtca tcaaaggaac ggtaatcacg ataaacatca
    15061 gttggtaaag aaagaacatt aaaaatggaa tcgttgcacc atgaaacatg ttggggaagc
    15121 ttgtaacatg tctcatcaaa aagaaatctg ccgggtttcc aataacaccg ccgatatccc
    15181 gtccaaaggc caatccgaat ccaccaaata cccacataat cgttgttatc ccaacggaga
    15241 gaaaagattg agacatgatc gttagtatat tattcttccg tgctaatccg ccgtaaaaaa
    15301 aagctagacc aggagtcatc aggcaaacca ttgctgtaca taaaatcatg aatgctgtat
    15361 ctcctgtatt gataggtaat gtattcattt ttttcgcctc ctttctacga ttacgagtat
    15421 aaaagaaggt gtagttgttt ttttcttctg tttttttgaa ttaatacttg catttataaa
    15481 agacagacaa ttaataaaag cttgatgtgt tgtgctcttt ttcgtttttc acacaaataa
    15541 agacaaaaaa taaacggtct tctttttata gaaaaccgtt tattttcgtc tttttttatt
    15601 tttaaagata ttttttcata aggaa
//
LOCUS       DARBFO010000059        15606 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000059 DARBFO010000000
VERSION     DARBFO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.060"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1040)
                     /locus_tag="SCK51_001974"
     CDS             complement(<1..1040)
                     /locus_tag="SCK51_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1252593.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGV"
     gene            complement(1252..2148)
                     /locus_tag="SCK51_001975"
     CDS             complement(1252..2148)
                     /locus_tag="SCK51_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1252594.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(2141..2821)
                     /locus_tag="SCK51_001976"
     CDS             complement(2141..2821)
                     /locus_tag="SCK51_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1252595.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(2822..3604)
                     /locus_tag="SCK51_001977"
     CDS             complement(2822..3604)
                     /locus_tag="SCK51_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1252596.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(3597..4514)
                     /locus_tag="SCK51_001978"
     CDS             complement(3597..4514)
                     /locus_tag="SCK51_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1252597.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(4678..5001)
                     /locus_tag="SCK51_001979"
     CDS             complement(4678..5001)
                     /locus_tag="SCK51_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252598.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            5789..9016
                     /gene="fss3"
                     /locus_tag="SCK51_001980"
     CDS             5789..9016
                     /gene="fss3"
                     /locus_tag="SCK51_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEG1252599.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            9129..9443
                     /locus_tag="SCK51_001981"
     CDS             9129..9443
                     /locus_tag="SCK51_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1252600.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            9456..9830
                     /locus_tag="SCK51_001982"
     CDS             9456..9830
                     /locus_tag="SCK51_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1252601.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            9840..10175
                     /locus_tag="SCK51_001983"
     CDS             9840..10175
                     /locus_tag="SCK51_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HEG1252602.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            10258..11598
                     /locus_tag="SCK51_001984"
     CDS             10258..11598
                     /locus_tag="SCK51_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG1252603.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            11676..12350
                     /locus_tag="SCK51_001985"
     CDS             11676..12350
                     /locus_tag="SCK51_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEG1252604.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            12343..12507
                     /locus_tag="SCK51_001986"
     CDS             12343..12507
                     /locus_tag="SCK51_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252605.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            12583..13725
                     /gene="dcm"
                     /locus_tag="SCK51_001987"
                     /pseudo
     CDS             12583..13725
                     /gene="dcm"
                     /locus_tag="SCK51_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            13715..14005
                     /locus_tag="SCK51_001988"
     CDS             13715..14005
                     /locus_tag="SCK51_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252606.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            14264..15448
                     /locus_tag="SCK51_001989"
     CDS             14264..15448
                     /locus_tag="SCK51_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG1252607.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            15445..15582
                     /locus_tag="SCK51_001990"
     CDS             15445..15582
                     /locus_tag="SCK51_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEG1252608.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         4965 a         2840 c         3185 g         4616 t
ORIGIN      
        1 actcccaatt tgagtctttt aactgctgca ttgagatgta atactagatt attgatcaat
       61 tcttcatcag gataaaattc atttaattta attcttacta actcttgtag ctcaacaccc
      121 atatctaaat aggaaagctt cgtttgatct aagtcgtgat tcatagcatt tttttcaatc
      181 gaagtaatgt gaatgtcaac ataagcttgt tcatctattg gcacaataat tgaaaatgct
      241 ttttcaatca ttttgattaa aataagtgta ttctttgaga tttcttcttc agaattatta
      301 ctacgtgata agaaattgct tgtttcaatc ctttctatcg caatagcaag gtggatagct
      361 aaagtttgaa attcataatc tgtaaatttc aattgtgtct tattcatgta tcgattaagc
      421 aattcggcta cttttttcaa tatgtcctta tttaagaaat tattcgcttc accaatgaaa
      481 ttgatattca agtctaagag atctgtttta ctgttagatg ctgtaaagcc accccaataa
      541 tagttcacaa cttctgacat caaccttctt ttactttttt catcagcttt gatgcgcacg
      601 ccatttcgat ctgaaactaa ggacatccca tgcttgttta gtctttttct aacatctttc
      661 attgtgcttt ctaccgtcga cctgctcacg tataattcgt cagcaagatt ttgaatcttt
      721 agagaatcat tagtattcaa taatttagca aacacataag ttagacggtc ctccggagag
      781 tctgcgatat tgattttatg actggataat aaatttcgaa gtgtttcttt ttttccaata
      841 agcgaaacgc cttttttagg ttgaaccaac atttgtacat ctggcatttt ttggattatt
      901 tctttcaaaa ttaaaatatc tttgctaatt gttttcgtag atacatataa ttgttctgat
      961 aattctttgt aagttgtgtg ctcattttca atcaaaaagt atagtatttt catttgccgt
     1021 tcgctaaatt cgatgcccat tatcttcacc tcctgaatac agcttaagtg tacaattatt
     1081 atctctattt tcatacagaa gaaagttctt gattaatgta gaagtttcta atattcttga
     1141 ttatttcaaa tcaataaaaa agcctgcaat atttatatca caggcttttt tctctattaa
     1201 atatatataa gataatacat ttttctacta gttctcacta atcttgtata attaaaccct
     1261 ccgcaacttg acggagatgg agaagatgcc cccgtctaat ctagcggtca tctctcctcc
     1321 cattccttcc accaattttt ttgacaggta aagcccgatt ccgctagatt ccgtatttgt
     1381 gcggctggta ttttcggtat aaaaacgctg gaagactttt tcaacagagg tttgcgttcc
     1441 tggtttgata ggattggcca ctgttaatcg aatgaatgga tcctcttttg aaaatgtcac
     1501 ctttgcttgt ccatctgcat aacgcaataa attctgtatg atattttgca gcactcgcag
     1561 caacatgagc ggatctgcta atacagttgt atttatcgag tgttcagcga acgctacctc
     1621 gatatttttc tcagaaaatt gttcataaaa agagaaaaat acttcttcca cctctttaga
     1681 aaaagaaagc gtatggagat ccatttcttt ttgcttcgtc tctattcgag caagatcata
     1741 aaactgatcc gtcatactga gtaatcttgc aacagattgt ttgatgatcg ttaaatactt
     1801 ctcccgtttc tccgtatcct ctacctgttc cagtaattgt acataaccgt taatagcagt
     1861 caaaggagtt ctgagatcat gagaaaggcc gacgatcgat agtctgatgt tttcttccat
     1921 ttcttgaaat gcttgtcctt tttgttcatt ttcatccaac catgtattca gtgcatctac
     1981 taggtccatt agattctttt ctcgaaattc gactgaaaga cgaccaccat attcttgtcg
     2041 ttcagaaaga gtggcgattt tcttagttaa tctcttgatt tccctttgca ggaagaaaat
     2101 aaaacttccc ataaagataa caacgagaag caacagccat ttaatcaaat ctaaacccca
     2161 ctccccacac cgtatgaata tacacagcat gtgcctttgc tagcttccct cgaagattgg
     2221 aaatatgcat attcactgtt ttctcactat caaaatatgg ttcttgccaa actgtttcat
     2281 aaagatttgc tcgcgaaaat acttttttcg gatgttctaa aaaaagcttc agcagatcga
     2341 attctcttcc tgtcaaatgg acagccacac cattcacatt tacatccctc gactctttgt
     2401 ccagtaagat ttccttgtaa tagatgcgtt gcgacagatc ttttacttgg acagaagtct
     2461 gatgacgaag ttggatgccg attcgtaaca aaagttcttc gatatcaaat ggtttcgtca
     2521 catagtcatc cgctcctgct gccaacaagg aaactttatc tctttgatcg ctttttgctg
     2581 aaagaacgat gatcggaaca ttcgatgtct gtcggatttc tgctaacaat gcttcacctg
     2641 aaagccctgg aagcatgaga tctagaagga tcaaatcgac aggatatgtg cttaacaata
     2701 attttccttc cgtacctgaa taagcatcaa gaagtttgta tctctgttca agaacctcct
     2761 ccagtaagga atgaacagct tcatcgtctt cgataatcaa aatagtttcc agcaggacca
     2821 cctacttaat gtcagatttt tggaaaataa acattcctag agctgatgat accacgattg
     2881 ttactagact gaccagaatc attttcggtg cttgcgaata agttgcttgt tgcgcaaagt
     2941 ccgtatacat gagccacgtt tccgtaaacc actccgttag aaattccaag cctttgattt
     3001 ttgcgaaaac accgggaatg aaggccgttc ccatgatata aagaaaagaa agaagcaaac
     3061 tgacactggt acttctagtg atgaatgtca aagccactgg aacagataat gtccctaatg
     3121 tcagcagaag caatacagga aatactttta gaacataatt tccatagttt ttgattccct
     3181 caacttcacc aaatccgaaa gcaagtgtgt aagcaagcat ggctagtccc gtgatcaaaa
     3241 ccacgccaag tgttgtgatc aaaagcgtaa cgagaagttt cgacacataa taagtcattc
     3301 gattccgtcc taaagataac gtattgcgaa tcgtaccgtt ttgaaattct tccccaatat
     3361 aaaagcacgc aaatgctgcg aaagccagaa gaatccatgc agccattttt tctaccatta
     3421 aaatccctgt tgcaccgttg atgactgtat ccgttgcttg gtttaattca ttgccgtatt
     3481 ttgtatgcat ataccattcg ttccaagaat aaaaaagcgg aagtacgacc atcagtccta
     3541 gtgagaccca cattttacgg ctcttccata cttttatttt ttcagcagtt aatagattaa
     3601 acatgtggat tgcctccagt taattgtaag aaataatctt ccagtttttg cccaactagg
     3661 tgaataccat ccacaggaac ttttgcttct accaatgctt gattgatggc tgctacttgg
     3721 tcaattcctt caaaaagctg gataacacgg ctagattgta caaaataatc cctataacca
     3781 agagagtcga gaaccgttac tgcttgttcc gtcgccgaag tgtcaatttt tataaattga
     3841 cgagattctt gtgccaattc ttctttcgat aattctttga tcaagtgtcc ttcatgcaaa
     3901 ataccatagt gagtggcgat ctgtgacaat tcatctagca agtggctaga aagcaagacg
     3961 gtgatgcccc gttcttttac caaacgttgg atgatttctc gcatttctgc catccctgct
     4021 ggatccatcc cattcgtcgg ttcatccaaa atcaaaaatt ctggattcgt gatcagtgca
     4081 tttgcaatag ctaaacgctg gcgcatacct aaagagaaat tctttgcttt ctttttccct
     4141 gtattttcca atcgcatcat tttcagcagg tcttcgattt ctctatccga cacaccgccg
     4201 ttcgctgctt ggatacgtag gttatctctt gcagttaatt ctggataaag agcaggggtt
     4261 tcaatcgatt gacccatacg tcgtcttgct gcctgcaaat ccgtcgttcc aaataattcg
     4321 atcgaacctt catcaatcga gatcaaaccc ataatagttc tcataaaggt agacttaccc
     4381 gctccatttt ccccaatcaa tccatagatc attccacgtt tgatttcgat tgatacttta
     4441 tctaatgctt tggcagcacc gtattttttg gttataccag ttgctttcaa gaccgtttca
     4501 tttccaaaat ccatacttat ccttctttct tattgatgag tgaagtatag ttgacgggta
     4561 taaagaaagc agccagaaat gtataaagaa agtgtaaatt ttccaattga atgtcaaaaa
     4621 aaacaatcgg acaatacgtg tctttcctat tatcaggcag tttgttatac gcttgtatca
     4681 gtttcctgat tctttctttt cgaccaaagt cgtttctctg tattcctctt cactgaaaat
     4741 ttttgcttct ttcagatcaa gcttgagtaa ttttaccaga ccggttataa ggataactat
     4801 agcaagactc cctcccagaa aaattagact gtctcgcaga cgtggttgcg ttgtcttcca
     4861 atcaaagagc gcaccaaaca gtgctaatcc aagtggactg gccaccatgg taatcgttgt
     4921 tatcgttgac ataagtcgtc cttgtatatg cgttggaact tctcgctgaa gccagaccat
     4981 ataaaaagca aaagaaaaca ttaatcctta aaaacggact tatgaaaaat aatacatgcc
     5041 gtttgggatt tacaattagc taccaaataa gaggttatga caaaagactt ttcacaatcc
     5101 cttcttccga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca caatatttat
     5161 caaacatgag aagcaatttt tgtaaattcc ttcttatttc ttgaagctga ccacttctgt
     5221 cacaacctca gaagcggata aataatattg tttgatgata ctttatcttt ttcccgtttg
     5281 tttatagatc gaatgatatt atagaccaag aatcctacct attaacatag ctaaaaacac
     5341 aaaaaaggca ctttccaaat tgtggaaagt gccagaaaag ttaggattaa ggtaactgcg
     5401 ctcagcattg cttcgcccag tacgattctc gtaactcgtg aactcgaacg ataatcgcaa
     5461 ttaacgtttt gagaactgtg aagctttacg ggctttctta agacctggtt ttttcttcgt
     5521 atcattgaat aagtttgaaa aaccttgatt tataaaggtt ttcccgtaaa tatattttat
     5581 tatatttgat taaatttgaa atgtttgtgt tctaaaatgt gttctaattt aaaggcagta
     5641 tatttcgtat tttagattaa atttttaggc tcattcattc tcaaatccat tctattcagt
     5701 caataaaaat tttaaaaaga ctttataaac aaagatgttc aagcgagcat cttatttttt
     5761 tgtgcttaat ttgaaaggag aacatttcat gtataaaaaa caaaaaaaga aattatgtgt
     5821 attaactatt ttgagtttgg ctgttcaagc aattgttgga gcaattgctc ctacgattac
     5881 ttttgctgat gaaattaccc atccacaaac ggtaaccgta gaactagatt tagcacatca
     5941 atatgctgtt gaaggaactt ttagtgatgg tcgtccaatg tcagaagtca ctgttccgca
     6001 ttatgcagtg tacaacggct ttaaacaaga tattttctgt attgaaccag gagtcccgat
     6061 ttacaatgag ttcactcctg gctatgagaa aaacccgtta ccagatatgt ccgataaagc
     6121 caaattagtt tccgttttat ggaaaaaagt aggtacagat gcagatactc atattgtggc
     6181 acagaagatg atctggcaag aagttaatgg gtataccctt cactcaataa aacgatcaga
     6241 tggtagtgcc gtaaatatcg cagccattga ggcaaaaata aatcaagcca ttgacgatta
     6301 tcagaagaaa ccaaccttcc ataatagtac ggctaaaaca gtattagggc aatcgactac
     6361 tgtgacggat acgaataatc tgaatttgtc agagttcgat gaggttgtgg aaaatgccgc
     6421 aaacattgat tatcgtgtga atggtaatca gttgataatc accccaaatg ccaattcaaa
     6481 agaaagtggt gtgctgacgc ttaagaaatc tgctggtacc ggaacacctg tcgcctataa
     6541 aaaggctggc caacagactt taatggccgg ggcgattgat aaacctaata cctacacaat
     6601 caaaattgat gtggaaacgg agggttcttt aaagatcaaa aaagtcgata aagaatcggg
     6661 tgatgttgta ccaggaacgg ttttccattt agactttgga aaaactttac ctgcaaaaga
     6721 cgtgactact gacaaagaag gcattgctac attggatgag attcctcatg gtacaaaagt
     6781 aaccattacc gaaaaatcag taccggcgcc ttatacgatt gataccaccc ccatgactac
     6841 caccattaaa gcaggcgaaa ccatttacgt gacttcaaaa aacgcacggg aaaaagggca
     6901 aatcattctg gataaaagcg gagttgaaac agggagtgat ctttggaatg acaattattc
     6961 cttagctggt aatacgtttg ccattcgcaa agacagtcca accggtgaaa tcgttcaaga
     7021 aatgaccacg gatgaaaacg gtcatgcgga aacaccaaaa gagattgcta atgcgttgga
     7081 attgggaacc tattatgtga ccgaaactaa agctagtcat gggttcgtga atacgttcaa
     7141 acctgtaaaa gtcgaattga agtatgccaa tcaaaccgta gcccctgtta ccagtaacgt
     7201 aaaagggcaa aatcaagaag tcactggcga aaccactttg acgaaagaag acaaagatac
     7261 tggggataaa gcacaaggta aggcagtctt tgagggtgct gaatacacct tgtttactgc
     7321 taaggatgga aaagctgtta aatggagtga ggcttttaaa ccagagttag cgaagggaac
     7381 gaaaggttct gatgaaacgg tcactttgac tttagacgaa aagaaccaag ttgccgttaa
     7441 acatttagct atcaacgagt attattggca agaaacaaaa gcgcccgaag gatatagcct
     7501 tgatgaaaca aagtatcctg tatccatcaa aaaggtggat gatagcgaaa aaaatgccgt
     7561 gattacccga aatgttacgg caaaagaaca agtgattcgc tttggctttg atttctttaa
     7621 atttgctgga tcagctgcgg gcactaccga aactggcttc aatgatctga cctttaaagt
     7681 gtcgccattg gaagggacca atgaaatcac aggtgccgaa gatgaagcga ccacagctta
     7741 taacgagcaa ttagggtttg atggttatgg caagtttgaa aatcttcctt atggggatta
     7801 tttacttgaa gaagtagaag ccccagaagg ttttcaaaag attacgccat tagaaatccg
     7861 ttctactttt aaggaaaaca aagaagactt tgtgaagagt gagtatgtct ttaccattac
     7921 cgaacaagac caaaaacaac caattaaaac ggtgaccgtt ccttacgaga agctgacgaa
     7981 caaagcattt tctgttagtt tgaaccgttt aatgctctat gatttgcctg aggaagaaga
     8041 tagtttaact tctcttgcga cttggaaaga cggaaacaaa gaattaactt ctctggattc
     8101 taccgagcta gttgataaat tgagttacaa tctacatgaa atcaaagaag actggtatgt
     8161 ggtagcgcaa gccattgatg tggatgcgac aaaagctgcc caagaaaaag atgaaaaagc
     8221 caaaccagtg gtgattgcag aaacaagcgc aacgttggcg aacaaagaga aaacaggaac
     8281 ttggaaaatt cagcataaat taaccgccga acaagtttta aataaaacta ttgtcttatt
     8341 caactatgtg tatgaaaata aggaagcttt tgaagcaggc gataagccag tagcgaagga
     8401 tgttagcttg aataaccaag cccaaactgt cagttgtaca gtggaacacc atgtttctat
     8461 ccaaacaaag gcccacctag aaaacggttc tcagaccttt actcatggtg atgtggtgga
     8521 tatgtttgat gatgtgtcga ttactcatga tgtactggat ggctcaaaag aagctttcga
     8581 aacaattctg tatgctttat taccggatgg tacaaacaaa gaaatttgga aatctggcaa
     8641 gattgattat gaagtaaatg ataaagaatt caccaaaact gtgcttgcga aaaaagtaga
     8701 taccggaaaa tatccagaag gaaccaagtt tacttttgcg gaaatcaatt acgataaaga
     8761 tgggaatatc aatggaaaac acaatgaaga tttgaaagaa aaatctcaaa ccttaacacc
     8821 aaaagaagtt ccaaccatac taagtacgcc gaaacaaccg gaaacaccaa ctgttccaag
     8881 tggctctcaa gaatctagtc ccacagtgaa gaccttccct caaaccgggg agaaaaattc
     8941 caatgtttta ctgttcattg gttttacttt gatttttgcg acggcgggct attatttctg
     9001 gaatcgccgg aactaaggtg atgagatgat acaagtaaat cctaagaacc aacgtgcgcg
     9061 accgcctcct aaaaaagtag tcgcgctatt tttgtatcca aaactaacaa tgaaaagagg
     9121 aaaagaagat ggaattgaaa tttgtcgtgc cagatatggc tgaaaccttt ggaaagttaa
     9181 gttatgccgg tgaaggagaa attataacag aaggatatgg acggaatact acggtgattg
     9241 gtcgcagtta tcatttgtat tccagtaagc aacgggccga tgatattgag gtggtagtag
     9301 ctgcggaagc cggtgaaaag gattttgatc aagaccaacc gctaaaagcc gtgaatcccc
     9361 atttggttgc caaaggctat gagattgaga atcgtgggtt tactgattac gtgttgtatg
     9421 ttgatgattt agtgaaagca taggaggaaa agaagatgag attaacagaa ggtattgtcg
     9481 tagattcagg tttaacattt gggaagttgc gtttttccgc attacgccga gaagtacgga
     9541 agcaaaatga ggatggaaca attggtaatg aggtaaaaga acgaacctat aatttaaaat
     9601 cctctgcaca agggcgaatg attcaagtta gtatcccagc taatgtaccc ttacgcgagt
     9661 ttgcttatga tgcagaggta gagttggtca atccaatcgt ggataccgtt gccaattatg
     9721 tttttcgaga aggaactacc gttaattggt tcattaaggc ggatgattta gtcttgaagc
     9781 ggcagccgaa tcaaggaaat cctacgaacc aaaatgaaag aaagaaatag gtgaagatta
     9841 tggaaattaa tgttgaacat attttagatt gtctcgatca atatggaaaa ggcgagctga
     9901 ctgaggaaca actcgttaaa gcattaacct ctgatgaaaa aatgtttctg attatacatc
     9961 aaggtttact tggagaaaat agcgacagtg aagaagactc tgatgtacta gactggttag
    10021 gagaagaaga gtctttcttt atagttgtcg aagtaaatga aaacttatgt cggcaggcag
    10081 aatccatttt ggaagaaatt ggtgtggaga tgccggatgc tattgaaggc tttttaaatc
    10141 aattggttga aacgaaacaa ctcccctcct cgtagcagta aatgactagc ataacttttt
    10201 gaaattgcct aatgaaatcc tattgggctt tttcgattgg aggtggaaac catcaaaatg
    10261 tacaaaggac atcgcatacg cgcaggagat cagcacttgg tttatcactt tgttcttggc
    10321 tggctactcg ctctgttcat cggttggatg agcgtctttt attttcaaga gtttagacaa
    10381 tttgatattt ctaagctatc actttctact attgaaatcg tttggtctat aaaagattta
    10441 gtatgtctgc ttggaagtct cgctttttca ggagctatga tattgcttta tattcacttt
    10501 tttcttgatc attggcgaag cttgtggcat cgacaaaagc tagctcggat gattctagag
    10561 aaccattggt atgaagtgaa acaaactcaa agtgaaggct ttttcaaaga tttgaatagt
    10621 agccgaacca gagagactat cagttacttc cccaaaatct gttatcgaat gaaagatgga
    10681 ttactgtcta ttcgcgttca aatttcactt gggaaatacc aagatcagct tttaaaattg
    10741 gaaaagaagt tagaaagcgg actgtattgt gagttagtag aaaaggaact caaagactcc
    10801 tatgtggagt atactttact gtatgatatg atcgccaatc gaattgggat agacgaagta
    10861 gtggcagaaa atgggactct gcgattgatg aaaaatcaag tatgggctta tgattcttta
    10921 ccccatatgt taatcgctgg tggcacgggt ggagggaaga cctatttcct cctcaccatt
    10981 atcgaagcac tattgaagtc agatgcagaa ttgtttatcc tcgatcccaa aaatgcagat
    11041 ttagctgatt taggtacggt gatgcctcat gtttattcac aaaaggaaga aatttctgct
    11101 tgtgtggaag atttctatga acgtatgatg gctcgtagca aggcaatgaa agaaatgtct
    11161 aactacaaaa caggagagaa ttatgcctat cttggacttc cacccaactt tttaatcttt
    11221 gatgaatacg tggcttatat ggagatgtta acgacgaaag aaagtgcggt gattttgaat
    11281 aagctcaaac aaatcgtgat gttgggtcgt caatctggtt tctttctgat tttggcttgc
    11341 caaaggccgg atgcgaaata tttaggagac ggaatccgcg atcaattcaa ctttcgcgtg
    11401 gctttgggtc gtatgagtga actcggatat tccatgatgt ttggggaggt cgaaaaaaac
    11461 ttctttatga agcacatcaa aggtcgaggt tatgtggata caggtggcag tgtgattagt
    11521 gagttttata cgccacttgt accaaaaggg tatgatttct tagattcaat tagtaaatta
    11581 gaaaagatga atgattgagt gatcttcaat actgacttta tacttcaagt aacgtataat
    11641 acttttatat agtaggaaga aaggggataa ttaatatgga aagtaatgat tcttatttag
    11701 agtatgcaaa agcaaataag gataagttta tttcttatat tatcaaaggg aaatcccccg
    11761 aagttggacg agacgcagtt tttatggcag ggagtcctgg ggctggaaaa tcagaggttg
    11821 ccattggatt agctgagaat tatgataacc atgtgattat tgatgcggat tactttcgca
    11881 cccagtttcc tgattataat ggaaaaaact ctagtctctt tcaaaaagca tcttcttggt
    11941 tggtagagca ggcatttaga tatgtcttag atcatggcta ttcttttatt ctggatgcaa
    12001 cttttgcaat attaagtgct gaaaaaaata tctcgcgggc gttgaaaaaa cattttcgag
    12061 tgacgatttt ctatgtttat caagacccta aaatcgcttg gaaatttaca caagaacgtg
    12121 aaattgcaga gggacgtcat gtgccgaaaa aggcatttat taacgctttt ttcaaatcaa
    12181 gagagaacat tgcaaaagta aagagtaaac atccagatgt attgcttcat atcataataa
    12241 aagattatac gaataatatt tccgaagttc attatgctac ggataatcta gaattatttt
    12301 taccactagg gtatagtagg aaagatttgg aggttcaatt acatgactga gaaagaacaa
    12361 gtaacgaaaa ttgtaaagaa atacaataag agcattgcgg atttatcgga aaatgctact
    12421 gcaaaagagt tcaaaacggt gattaaatat gtagccgatc aggccaatga aaaacaacgg
    12481 aaattagttg gattaaataa aaaatagtag gacaaattaa taatgtatga gccaagagca
    12541 aaatgttctt ggcttttttc tttgcaatga atgaggtaat gtatgagatt tttagattta
    12601 ttcgccggta ttggtggctt tcgtttaggt atggaacagg cgagccatca ttgtatcggt
    12661 ttttgtgaaa ttgataaatt tgctagacgt agttataaag caattcatga tacaagtaag
    12721 gaggtggaaa tgcatgacat cacaagtgta tcagatgagt ttattcaatc cctcggacca
    12781 gtggacatcc tttgcggcgg atttccgtgc caagcttttt caattgcggg aaagcggcaa
    12841 agattttccg atactcgagg tactttattc tttgaaatcg ctcggttcac cactcttctc
    12901 cgacctaagc ttttattcct tgagaacgtc cgaggattgc tcaatcacga aggaggggct
    12961 acgttcgaga cgatcctccg aacgctggat ggattggggt atgacgtgga gtggtaggtg
    13021 cttaactcaa aggcctacat tccccaaagc cgggagcgga tattccttat cggacattct
    13081 cgagacgcat gtaccgaaca agtatttcct atcattggac cgtcttcaac atctgatcaa
    13141 aacatcagaa atttggtaaa tatcaatcct tccaatcgag gaatgggtgg acaagtctat
    13201 ggttcagatg gcgtagcgcc aaccttaaca ggtgatgaag gaatcaaaat cgcactgcca
    13261 gcaagggatg gaatctctgt agcaggacta ttaccgggaa agtttgaaca aggtaatcga
    13321 gtttatgaag ttactggaac cgcccccacc ttgtcaacga aacaaggtgg tacaaaaatc
    13381 atgattcgaa aaaatgaaac agaatatcaa gaggtgaagc cagaagacag tgtgaatctg
    13441 gcttttccta attccacaag tcgccgaggt cgtctgggaa aacagagtgt tcatacctta
    13501 ctaacaggag accaacaagc gatcgtcacg gatcagtatc aaattcggaa gctaacacct
    13561 agagagtgtt ggcgacttca gggctttccc gattgggctt ttgatcgaga ttctcaagtc
    13621 aattcagata gtcagttgta taagcaagcc ggaaactctg taacagttcc ggtcattttt
    13681 gatattgcca gaagattaaa agagttggat gtacatggtt tatgatgagt tgattggaga
    13741 aatttattgg gtgattggaa agattcagtc agacccagaa ttagaagaac aattgcgacg
    13801 gttaaaattt gatattcgca agaatggtgt caaagtcgct ggcgatccat atttaatgaa
    13861 tgaggagacg gatgctcgaa ttgaaataaa tcaagtaatt gcagaatttg agcggattgc
    13921 ggaccaagca aaagaaccag atattcgtca atacttgtta gaaatgaaag cagagttgga
    13981 agtaaatggt attacagatg agtaacctaa accaacacaa ctgactgtta ggcgctgacc
    14041 gcggaggatt cgcgaagcag aaatcctccg tggtgctgtg cctgttcatg gacggcgtta
    14101 gccgaccatg atgtctcacc cccgtatcta acaggggggt acaaaaacac aaaaaataag
    14161 taagcaacca ttaagaatgc ttgttgtatc aaagatagta agtaatttgt ataggtgtta
    14221 actcatttga aaaagttaac accttgaatt tggaggtgag aacttggcac aacggaattt
    14281 agattatcgg ctattaaaag accgacgcaa cgaatacggc gtttcacaaa ataaattggc
    14341 tacggcttgt ggacttagtc ggccgtactt aaatcagata gaaaatggtg gcgtaaccgc
    14401 atctataaaa accatgagga aaatttttga ccaactggaa agctttaatc ccgatttgcc
    14461 tttgacgtta ctgtttgact atgtaaggat tcgctttccc acaacggatg cacgaaaaat
    14521 tattcaagaa attctccatt tgaaatttga gtatatgctc catgaagatt atgcctttta
    14581 ctcctatcaa gaacagtatg tcatgggtga tattgtggtg atgttgtctc atgaagaaga
    14641 taaaggtgtg cttttagaat taaaggggcg aggttgccga caatttgaaa cttttttact
    14701 cgctcaaaag cgtagctggt acgacttttt cgaggattgt ctaaaggccg gtggcgtgat
    14761 gaaacgcttg gacttggcaa tcaatgatcg agtggggcta ttggatatac ctgagctaac
    14821 gaaaaaatgt cagaaggaag aatgtatctc cttgtttcgt acctttaaaa gttatcgttc
    14881 cggggaactt ttaaaggctg atgaaaagga cggcatgggc aataccttat atatcggcag
    14941 tctcaagagt gaggtttatt tctgtttgta tgaaaaagac tatgaacaat atatcaagtt
    15001 agggattcca ttagaccaaa ctgagacgaa aaatcgtttt gagattcgac tgaaaaatga
    15061 ccgtgctttt catgcgattc aagatttgtt aaaaggtcgt agcattgaga gcaccacgtt
    15121 ttccattatc aatcgttact tgcgatttgc ggataaagta gaaggtaaaa ggcgaaccaa
    15181 ctggcccttg aatgaacaat gggggcgttt tattggacgg aatcggaaag agattcaatt
    15241 aacttccgag ccaaaaccgt ataccattga acgaaccttg aattggctag gccgacaagt
    15301 cgcccctacg tggaaaatgg ctaaggagtt ggatcgattg aatcggacaa cctatatcca
    15361 ggatatggta cagaatgcac gcttatcaga ccgacataaa aagattttgg agcaacaaag
    15421 tatggcaatt gaaaatttga ttatatgaaa agaggaatcg tcatgggaat tgtaaaagat
    15481 atttttttgg tatttggtgg cagtatgctt ggtgttactg tgatgtgctt gatgaatgca
    15541 agtgcagcag cggatcgagc aatggaaaag aaaggaaagt gacaagatga atttcggaca
    15601 aaattt
//
LOCUS       DARBFO010000060        15230 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000060 DARBFO010000000
VERSION     DARBFO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.061"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            278..787
                     /locus_tag="SCK51_001991"
     CDS             278..787
                     /locus_tag="SCK51_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1252609.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="SCK51_001992"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="SCK51_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1252610.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="SCK51_001993"
     CDS             2322..3302
                     /locus_tag="SCK51_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1252611.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="SCK51_001994"
     CDS             3424..4842
                     /locus_tag="SCK51_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEG1252612.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="SCK51_001995"
     CDS             4913..6382
                     /locus_tag="SCK51_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1252613.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="SCK51_001996"
     CDS             6392..6859
                     /locus_tag="SCK51_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1252614.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="SCK51_001997"
     CDS             6895..7767
                     /locus_tag="SCK51_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1252615.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="SCK51_001998"
     CDS             7848..10382
                     /locus_tag="SCK51_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1252616.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="SCK51_001999"
     CDS             10538..11311
                     /locus_tag="SCK51_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1252617.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="SCK51_002000"
     CDS             11304..12095
                     /locus_tag="SCK51_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1252618.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="SCK51_002001"
     CDS             12105..12596
                     /locus_tag="SCK51_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1252619.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="SCK51_002002"
     CDS             12614..13024
                     /locus_tag="SCK51_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1252620.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="SCK51_002003"
     CDS             13028..14428
                     /locus_tag="SCK51_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HEG1252621.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="SCK51_002004"
     CDS             14451..14825
                     /locus_tag="SCK51_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1252622.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5286 a         2135 c         3046 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag
//
LOCUS       DARBFO010000061        15115 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000061 DARBFO010000000
VERSION     DARBFO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.062"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            118..2715
                     /gene="adhE"
                     /locus_tag="SCK51_002005"
                     /gene_synonym="adhC"
     CDS             118..2715
                     /gene="adhE"
                     /locus_tag="SCK51_002005"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEG1252623.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2944..4050
                     /locus_tag="SCK51_002006"
     CDS             2944..4050
                     /locus_tag="SCK51_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEG1252624.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4067..5095
                     /locus_tag="SCK51_002007"
     CDS             4067..5095
                     /locus_tag="SCK51_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEG1252625.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5101..5580
                     /locus_tag="SCK51_002008"
     CDS             5101..5580
                     /locus_tag="SCK51_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEG1252626.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5596..6891
                     /locus_tag="SCK51_002009"
     CDS             5596..6891
                     /locus_tag="SCK51_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEG1252627.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6902..7726
                     /locus_tag="SCK51_002010"
     CDS             6902..7726
                     /locus_tag="SCK51_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG1252628.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7891..8790
                     /locus_tag="SCK51_002011"
     CDS             7891..8790
                     /locus_tag="SCK51_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1252629.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8916..9785)
                     /locus_tag="SCK51_002012"
     CDS             complement(8916..9785)
                     /locus_tag="SCK51_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEG1252630.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9938..11398
                     /gene="rhaB"
                     /locus_tag="SCK51_002013"
     CDS             9938..11398
                     /gene="rhaB"
                     /locus_tag="SCK51_002013"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEG1252631.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11395..12651
                     /gene="rhaA"
                     /locus_tag="SCK51_002014"
     CDS             11395..12651
                     /gene="rhaA"
                     /locus_tag="SCK51_002014"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEG1252632.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12665..13510
                     /gene="rhaD"
                     /locus_tag="SCK51_002015"
     CDS             12665..13510
                     /gene="rhaD"
                     /locus_tag="SCK51_002015"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEG1252633.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13512..13823
                     /gene="rhaM"
                     /locus_tag="SCK51_002016"
     CDS             13512..13823
                     /gene="rhaM"
                     /locus_tag="SCK51_002016"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEG1252634.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13905..15056
                     /gene="fucO"
                     /locus_tag="SCK51_002017"
     CDS             13905..15056
                     /gene="fucO"
                     /locus_tag="SCK51_002017"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HEG1252635.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
BASE COUNT         4865 a         2593 c         3420 g         4237 t
ORIGIN      
        1 tatttaaaaa taactgtgaa aaaaatcaca aaaaagtgtt tacatagaaa aataaatatt
       61 gtatgatata catgtgaatt atttaacaaa taaccatgac ccattaggag gataatgatg
      121 gctaaagtaa tagaaaaaga aaaaacgaag acaattgacg tacaagcaat gattgatgat
      181 ttggcagaaa aagcaaatgc agcattgaaa gaaatggaaa gctttgatca agaaaaagta
      241 gaccatatcg tgcatgaaat ggcaatggca gctttggatc aacatatgcc attagcgaag
      301 atggctgtag aggaaacagg ccgcggtgtt tacgaagata aagcaatcaa aaatatgtat
      361 gcgtccgaat atatttggca taatatcaaa catgataaaa cagttggtgt gatcaatgaa
      421 gatgtccaaa aagggttgat tgaaattgct gaaccagtcg gtgtggtttg cggagtaact
      481 ccaacaacga atccgacttc cacaacgatt ttcaaatcaa tgatcgcgtt gaaaacaaga
      541 aatccaatcg tttttgcttt ccatccaagt gcccaaaaat cttctgcaga agcagcacga
      601 gtggtacgtg atgctgctat cgctgccgga gctcctgaaa attgtatcca atggatcgaa
      661 catccttcga tcgaagcgac ttctatgcta atgaaccatc caggtatcgc gatcgtctta
      721 gcgacaggtg gagctggaat ggtcaagtct gcttactcta caggaaaacc agctttaggt
      781 gtaggtccag gtaacgtacc agcttatatt gaaaaaacag caaaaattaa acgtgcagtg
      841 aacgatttga tcgtttcgaa aacatttgat aatggaatga tttgtgcttc tgaacaagct
      901 gtgatcgtgg ataaagaaat ctatgcagca gtaaaagcag aattccaagc acaccaagtt
      961 tatatcgtaa aaccagatga attgcaaaaa ttagaagatg cagtgatgaa cgaaggaaaa
     1021 tatgcggtca atccatcaat cgttggacat tctgcgatgg aaatcgcaaa attggcagga
     1081 atcagtgtac caaaaggaac gaaaatgctg attgctgaat tggaaggcgt aggcccagat
     1141 tatccgctat caagagaaaa actttctcca gttcttgcca tgatcaaagc taacaacacg
     1201 gaccatgcgt ttgatttgtg tgaaggtatg ttgaatcttg gaggattagg acatacagca
     1261 gttatccatt cagaaaacga agagttgcat gtcaaattcg gtcttcgaat gaaagcttgt
     1321 cgtattttag tgaatacacc atctgcagaa ggcggcatcg gtgatatcta taacgaaatg
     1381 attccgtcat tgactttggg atgcggttct tatggtaaga actctgtttc tcgtaacgtc
     1441 tctgctgtaa acttaatcaa tgtcaaaact gtagcgaaac ggagaaataa catgcaatgg
     1501 ttcaaattac cgccaaaaat cttctttgaa aagaactctc tattgtactt agagaaaatg
     1561 gaaaacgttg aacgtgtcat gatcgtttgt gaccctggta tggtccaatt cggttactgc
     1621 gatacagttc gtgaagtatt ggcacgacgt aaaaatgatg tgaagatcga agtcttctca
     1681 gatgttgaac cgaacccatc gacaaatacc gtatacgcag gaacgaaact gatggcagac
     1741 ttcaagccag atacagtaat cgctcttgga ggcggttctg caatggacgc agctaaggga
     1801 atgtggatgt tctatgaaca cccagacaca tctttctttg gtgcaaaaca aaaattcttg
     1861 gatatccgta aacggacata taagatcgac aaaccagaaa aaacacaatt cgtatgtatc
     1921 ccgacaacat cagggactgg ttctgaagta acgccatttg cggtcatcac tgacagtgaa
     1981 acacatgtga aatatccgtt agcagattat gcattgacgc cagatgtcgc aatcgtcgat
     2041 cctcaattcg tcatgtcagt tccagcatct gtcacagcag ataccgggat ggatgtgtta
     2101 acacatgcaa tcgaatccta tgtttcagtc atggcttcag attatacacg tggattgagc
     2161 ctacaagcaa tcaagttagt ctttgactat ttagaaaaat cagtaaaaac acctgatatg
     2221 gaatcacgtg aaaaaatgca caacgcttct acaatggctg gtatggcgtt tgctaacgca
     2281 ttcctaggta tttgtcactc agtcgcacat aaaatcggcg gggaatacgg gatccctcat
     2341 ggacggacaa atgcgattct tttaccgcat attatccgct ataatgcaaa agatccacaa
     2401 aaacatgcga tgttccctaa atatgactat ttccgtgcag atacagatta cgcagatatc
     2461 gcaaaattct taggtctgaa aggcgaaaca acagaggaat tagtcgaagc tttagcaacg
     2521 gctgtttatg aattaggaaa atctgtcggc atcaacatga gtctaaaagc tcaaggagtc
     2581 acacaagaaa cactagatac aaccgtagac cgtatggctg aattagctta cgaagaccaa
     2641 tgtacgacag ctaatccgaa agaaccattg atctctgaat tgaaacagat catcattgat
     2701 gcttacaacg gataagcaaa agaaagcgta gaaaaaagat aagaaaatag atgaaaaaga
     2761 acttggaaaa cgttgtttct ccaagttctt tttttaattt ccaagtaact gaaagttact
     2821 tttgttattt aacatttagg tcaattttag acagtttttg ttcaaaaagc gatatttata
     2881 gtggacggta ttactgctat attaaggata taaaatgaaa gcgtttaaaa aggtggcgta
     2941 aagatgatta ttaaagaaga gatttttgta cctaaagaat tagtgaaaga aaaaattgac
     3001 ctgctcgttg aaaacctgac aacgatcaaa gacgataatg gtgaattttt gttggacttt
     3061 gatggactga aagtagatga caaaagctgg acagtgtgga attggccaca aggtgtcggt
     3121 ctatatggta tttataaaaa ttaccagatg acaaaaaatc ctcgcgctta tcaagtggtc
     3181 aatgaatggt ttgaggatcg tatggaagaa ggcgctccgc ccaaaaacgt caatacaatg
     3241 gcaccgctat tgactatggc ttacttgtat gaagacacga aagattcacg ctatttgcct
     3301 tatttagaac aatgggcaga atgggtcatg aatgacatgc ctcggacaaa tgaagacggg
     3361 ttacagcatg ccacatatgg accggaaaat aagaatcagt tgtgggacga tacattgatg
     3421 atgacagtgc ttcctttggc aaaaatcggg aaactgttga atcgcccaga atatttagaa
     3481 gaagctcgtt accagtttat gatccatatc aaatacttga tggataaacg aacaggattg
     3541 tggtatcacg gctggacgtt tgaaggaaat cataactatg ccgaagcatt ttgggctcgt
     3601 ggaaactgct ggttgacgat tgctattcca gagatcattg aaatcttaga attatcgaaa
     3661 gaagatgcat tgcgcaaatt attgatcgaa acacttgaag cacaagtgcg tgcattaaaa
     3721 acttatcagt ctgaaagcgg tttatggcat acgttgttgg atgatccttc ttcttacgta
     3781 gaatcttctg caactgcagg atttgcttac ggtattttga aagcggtcca taaacgttat
     3841 ttaccacaag aatataaaga agtcgcttat cgtgctatcc aaggattgct tgaacaaatc
     3901 gatgaaaaag gagaagttca aaacgtttca atcgggacag gaatggggga cagtttagat
     3961 ttttatcgga atatcggaat cacagcaatg ccttatggac agtcgttaac ggtgctttgt
     4021 ttgacagaat tgctcgtctc ttattgttaa aagaaaggaa acccatatga aaaaaacatc
     4081 actgctgttt gcagcaagtt cgcttagcct aggtctgcta ggtctaagcg gctgcggaaa
     4141 taattctagt gtggcaactg tcgcagatgc agatgtaaat gagatagtta ccttgcgctt
     4201 tgcttatgca agcaacagcc agccagtaat tgatgcaatg aatgaatttg ggcgtttagt
     4261 aaaagaaaaa acagatggtt ccgtacagat ccagtatttc ccagatggac aactgggtgg
     4321 agaacgtgaa ctgatcgaat tgacacagtc aggagcagta gatttcacaa aagtaagtgc
     4381 ctcagcgttg gaaagtttct ctaaagatta ttcgattttt tcagttcctt atctattcaa
     4441 tgatgaagaa catttcttta aggtgatgga taatgaagaa ataatggagc caatctacca
     4501 atcgaccaaa aatttaggat ttaccggatt gacctattat gattctggtc agcgcagctt
     4561 ttatatggta gacggtccga tccatacgcc ggaagatttg aagggaaaga agatccgtgt
     4621 gatgcaaagc gaaaccgcta tcaaaatggt agagctttta ggcggttcgc ccgtacctat
     4681 gggaagtgat gaagtgtaca cgtctcttca atccaatttg atcaacggat cagaaaacaa
     4741 tgaatttgtc ttgtatacag ctggacatgg tggtgtggct aaatactatt cctatgatga
     4801 gcatacacgt gtgccagata tcatcatcat gaatgatgcc atcaaagaac agctaactga
     4861 tgagcaggaa aaagccattg aagaagcagc gaaagaatca acggtttttg aaattgaagc
     4921 ttttgcaaaa gcaattgaag aagaaaaagc tttagcacaa gaagaacatg gagtccaatt
     4981 caatgatgta gataatacac cgtttcgaga ggcagttgcc ccacttcatg atatattcaa
     5041 aaacgatcct acctacaaag acctttatca aaagatccaa gagcaaggag cgtagctgtg
     5101 atgaaaatga ttttaaacaa ggtattagag atcctgggag ctgttttgct tgttgggatg
     5161 gtcgctgttg ttttatggca agtattttcc agaacggttt taggaaatcc aaatacggtc
     5221 acggaagagc ttgttcgatt cggtcttgtc tggtttgcca tgttgtcttc cgcgtatgtt
     5281 gtgggacaaa aagcacattt agccgtaacg gttttaagtg aaaaattaac tggcaaaaaa
     5341 agtaaaattt tagaagttgt tgttcaaagt ttatttctgg tatttgctgc gatgatcatg
     5401 gtgtatggcg gatggaatgc tgtcacattg acaatggggc agatctcgcc ttctttaagt
     5461 attcctatgg gatatgttta tctgtcagta cctgtatcgg gggtactcat cattatctat
     5521 agcctgatca atctaatcga tacattaagc aacaagccag caattcaaga aaaaatataa
     5581 aaatggagga ataaaatggc aatagaagcc ggattagtgt tattttttgt atttgttgtg
     5641 ttgcttgtat tagggatgcc gattgctata agtattgcat tctcatcgat gatgacttta
     5701 ttgttagtta taccgtttga tgtttctgct tttagttcag ctcaaaaaat ggtgggaagt
     5761 atcaatagtt tttcgctgat cgctattccg tttttcgttc tctcgggcat tatcatgaat
     5821 aatggaggga tagctaagaa actagtggat tttgcgatgt tgttcgtcgg gcgcgtgccg
     5881 ggggctttgg ctcatacgaa tgtgttaggg aatgctttat ttggttccat ctctagctcg
     5941 gccattgccg cttctaccgc gattggcgga gtgttgatcc ctcagcaagt ggaagaagga
     6001 tatgaccgga agtttgcgac agcggtcaat atcgcgtcag ctccgacagg tatggtcatt
     6061 cctccaagta ctgcttttat catgtattca cttgttgctg gtggtgcttc gatctctgct
     6121 ttgtttatgg gaggttatct cgtaggcgct ttgtgggcgc taggaatcat gctagtagca
     6181 ttcatcgttg ctaaaaagaa taaataccct gttgtcgaaa aaggacagat ggctcatgct
     6241 ggaaaaataa taaaagaggc gattcctagc cttctattga ttgtcatcat tatcggtggt
     6301 atcttgacgg ggatatttac agcagtagaa gcttcagcga tagctgtcat ttactccctg
     6361 ttgattgcta tgttttacta taaaacggta aaactaaaag atatacctaa tatgttgttt
     6421 caatcagtag caatgtcagg gacgatcatg ttcttattgg ctacttcttc catgatgtct
     6481 ttcgccatgg cgtttaccgg tattcctcag gcaattagtt cgttgattat gggcgtaacg
     6541 gataataaat ttattattct gttattagtc aatcttgtgt tattactggt gggtatgttt
     6601 atggatgtgg cgcctgctat cttgattttt acaccaatat ttttaccaat cgtcacaagt
     6661 gtaggggttg atcctgtaca ttacgggttg ttttcaatca tgaatctgtg tgtaggctcc
     6721 atcacgccgc cagtaggtac aggactatat gtcggtgcaa gtgtaggagg cgtaaaagca
     6781 gaggcaatgc tgaaaccgct tcttcctttt tatggggtta tcttagtcgt cttgtttttg
     6841 attacttact tcccgcaaat cgtcatgtgg ctacccaata tgctgagtta ggagaatagt
     6901 tatgttgaac attacaatta gaggacatga tctttccgaa gtccaaacga tagaagatct
     6961 tgcagaaaaa acaaaagaac aagggattca tacactgcag ctcgccttag gtctttcgtt
     7021 tcctgaaata ccttctggcg caaatgagat caattctggg atgggaaatt atgtcaaacg
     7081 agttttagaa aagcaagaag tgaccgtagg aattttaagt tgctatatca atatgattca
     7141 tccagattta acgatacgac aagagttgct gacaaagttc gagcagtatc ttcgctatgc
     7201 ttcttccttt ggtgcgtcga tggttgcttc ggaaactgga tgtgttttgc cggaaatcca
     7261 atatacggaa gaaaatttca cagatgaagc atttgctgaa gctgtgtccg ttatccgtcg
     7321 cttggtaaaa gctggagaaa aataccaaat gatggtgggg atagaaccag gtttgaatca
     7381 tccggtgtat tctttagcaa gggtagagca gctgatccaa gcagtagatt cggactatct
     7441 aggaatcatt ttagatccga ccaatttgat cacaagtagt gattatcaag aacaagtcca
     7501 attggtagaa gaagcttttg aacgttttgg tgaaaagata tgtgcggttc atttgaaaga
     7561 ttttcgtgtc gaacaggaaa aaatcgtgcc ggtaaatttg ggagacggtg tgatcgagta
     7621 cacaaaaatc aaagaaatca tcatgaagaa tcgtccgtac ctgtatgtgg ttcttgaaga
     7681 aacaaaagat gatgggattc gttatggaag aagtcttctt gaataatgta aagaagacgt
     7741 tggttaacta atatttagaa tggagggggc ggggctagaa gatgccacgt ctcttctttt
     7801 ttataagagt atataaaagc agctcttttt gattacaaaa aacgtggtat aataaaattg
     7861 tgcataagtg aacaaaagga ggtgcggcac atgctccaaa agaaaatcag cggtgaacaa
     7921 gtcaatacga tggtttctga tcagtatgaa atctatcatg tcaaagatgt cgtcagccaa
     7981 aataaaacga tctatcatca tcatgatttc tatgagatcc atgctacatt aaagggagaa
     8041 gcgacttttt atttgaacgg acgacaattt acgatcaaag caggaagtgt gctgctcatc
     8101 cattccaatg atttgcatcg tatcgtccgc caatcttcag atgtattcga gcgtgtctat
     8161 atctttgtca ccccttcttt tctagaaagt cgttccacgc catggtctaa tctatcggct
     8221 tgttttcaac caatgggaga gaagaggagt cgtgtattgc aaatagaaca ggaagtattg
     8281 aagcaaaaat tatcatttat cgaccatcct cctgataaaa aaatttacgg tgaagacata
     8341 aaatatgaac aagctctggt agaatacttg atttttctca atcaagttgt acaaaaagaa
     8401 gagagtgcgc aagaacagaa tgctgtagtt cctaatgagc gtgtggaaca aatgttacaa
     8461 tatatctcaa aaaatttatc tcatccgctt tctttagagg aaatggaaaa gcagtttttt
     8521 gtcagcaaat accatatcac acgagaattt aaaaagtaca ctggatttac gtttcatcag
     8581 tatgtaatga agaaaaagct gatctatgcc aagcacttgt tgcgagaata ccgcagctct
     8641 agtgctgtat atagtaaaag tggtttttct tcttatccgc attttctgaa agcattcaag
     8701 agagagttcg gcataacgcc taaagaattt ttgaaacgtg atacacagaa acagttggtt
     8761 cattatgaac attttgaaga tgaacaatga gaatgatcac taaaaaataa ccgcagtagc
     8821 tgctgttgtt tacatagaag cagaaactgc ggttatttta tttttatcta tatatttcaa
     8881 aatattttga aaaaatgacg tacatattat atgaatcagc tgaatttacg aaagtctctc
     8941 ggcgatagtc ctgtttttgc tttgaacagg cggctaaagt aagcaaaatt ctcaatccct
     9001 atttcttcgg aaatcattcg aaccgtaaat tctgtatacc gaagaaggtc tttagcgtaa
     9061 gaaagttttt tgctgatcaa ataatcgatt ggtggagaac cgataaattt ggaaaaatct
     9121 ttgctcagct gatatttatt caataagaat tgttgttcta attgatcaag tgtaatcgtt
     9181 tctttgaaat gttcgtctaa gtattgcttc agtttgtaaa cgtaggcggg aatatcctct
     9241 tcttcgaaat ctagctgata cttttgcaaa atcagttcat tcagcaactc gtggatagct
     9301 acagaggact ggaaatcggt acgggcattc gtgccttgtt gcatgtggat caattctgtg
     9361 atcaaatggt ggatcttgtt ttctgatgga tctcctgttg aatggaaggt gttatggccg
     9421 tcttttgtga actcctgata aaatgattgg acgttctgac cattcaaatg gatccaatcc
     9481 atttcccatg gttcttcgct gatcgtttga taacgctgat attcttggca gtcaataaaa
     9541 aataagtctc cagcttttag ctgttgctga ttatcattat atgtaagcga acctttgcca
     9601 gacaaagtaa actttatcaa gtaagaaggt aaatgtgctc gttcagtata gtaagggatt
     9661 ggtgctttga agtaaccgat ttcttggata taaaaaaata aattcctgct tctttgtgta
     9721 ggcgtcaaga tcactctttt tgagtcttcc gaccatgagt ggttggcttt ttcccagtaa
     9781 tccatgtaaa cactcccaac ttgtcaagat aatgcaatta tataccaaaa tagtgtgaag
     9841 caaaatataa gattctgtat tatgatagca ttgtcaataa atgataacgc tttattaata
     9901 aagtgctaat caacaataaa gtaaaagagg tggagaaatg aatccaacat ttgttgccat
     9961 cgatatcggt gcatcaagcg gacgactaat gaaaagtacg agggatgcaa aaggttcttt
    10021 gatcttggaa gaagtacacc ggttcaaaaa cggctttcgg aacaaagacg gtcacgatcg
    10081 ctggaagatc gattatctta tcaaagagat tttgattgga tttgaaaaat taaagcagac
    10141 agggattgat cgcctttttg cggggatcga tacatgggca gtcgattact gtctgattga
    10201 tgaacaaggg gatcggataa atgatccgat tgcttatcgg gacaagcgta ccaatggagc
    10261 agtagaacag ttttctgaaa tctattctct agataagttg tatgagcaga cagggattca
    10321 gatccagcct ttcaatacct tgttccaatt gtttgctgaa gaaaaagaaa atttggcaaa
    10381 agcacataaa ttgttactga tacctgatta tttagggtat gtctttactg gtaacatggt
    10441 gacggagaaa actaatgcat ctactatgca gctgctgaat gcagggacaa aagaatggga
    10501 aatagaactt cttgaaaaaa tcggtgtctc gaaggaatta tttccaccat tgacagagcc
    10561 gggaacgata ctaggagatc tgcaaaaaga acaattttca aaatatgatt tgccagatgt
    10621 gacctttatc acagtagcaa gtcatgatac ggcgtcagct gtattaggaa cgccaggtca
    10681 aggagaacgt tggggttata tcagcagtgg gacatggtcg cttttaggga ttgaaacaaa
    10741 gatcgccaat gtttctcgca atgctttttc tgaaaattat acgaatgaat ggggtgccca
    10801 taacacggtg cgttttttga aaaacatcat ggggatgtgg ttgatccagg aagttgcccg
    10861 aatgcaagag tacgagtaca gttatgcgga attggcagag cttgctgcaa aagaaaaacc
    10921 gctgcagcaa gtgatggaaa tcaatgaccc tcgatttttg aatcccccaa atatgataga
    10981 agagattcag caatactgtt ttgaaacaga gcagccagtc cctgtctcat cgggagctat
    11041 cgctcgttgt gtgtatgata atcttgctct ttgttacgca caagagctga tcaaagtaga
    11101 acacttaact ggaacgaaat tggaaaagct gcacatcgta gggggcggat cgaataatcg
    11161 tctactgaat caattgacag cagattatgc cggaatcatt gtagaagcag gacctagtga
    11221 agccacagca atcgggaatg ttgccatgca aatgctcagt acaggtcagt tttcgaatat
    11281 agaacaagca agaaatgtaa tcaaacagtc gtttccatgt gaaacttttt acccagatga
    11341 aaataagcaa aacacgatgg aatggtacgt gaataaaatc aaaggagtaa tcgtatgaac
    11401 acgattgaaa gaaattacga acaagcaaaa gaaaaatatg caactatcgg tgtagataca
    11461 gatgctgtac tagaaaagat acaggacatc aaaatttcta tgcattgctg gcaaggagat
    11521 gatgtcaaag gtttcttgac accggatggc gaattgaccg gtggaatcat ggcgacaggg
    11581 aattttccag gagccgcacg aacacctgaa gagcttagac aagatctgga gaaggcttat
    11641 tcgttgatac cgggcaaaca caaattgaac cttcatgcta tctatttgga tacagaagaa
    11701 tctgtcgatc taaatgaaat tgagccgaaa cattttgaaa aatgggtaga gtgggcaaaa
    11761 aaagaagaaa tcggtcttga cttcaatccg actttctttt cgcatccaat gatgaaagac
    11821 ggctttacat tggctcatcc agataaagaa gttcgtgaat actggatcga acacgggaaa
    11881 agaagccgca aaatcgcgga gtactttggg aaagaaactg ggcaaacaag tatcaataac
    11941 ttttgggtac ctgatgggat gaaagataat ccgattgatc gttttacccc acgggaacgt
    12001 ttaatggaat cactcgatga aatttttaca gaagagttag atgaacggta tgtacaagat
    12061 gcagtagaaa gcaaactatt tggtttaggc gctgaagctt atacagtcgg ttcccatgaa
    12121 ttttatatgg gttacgggtt gactcgtgac aaacttgttt gtctagatgc tggacatttt
    12181 catccaacag aagtgatttc caataaatta tcttcattag ctttattcag caaaggaatc
    12241 atgttgcatg tttcccgtcc agtgcgttgg gattcagacc atgtggtttt gatggatgat
    12301 gaattgcagg aaatcgctaa agaattagtc cgtaacgaat tgttggaaaa aacaaatatc
    12361 ggtctggatt tctttgatgc aacgatcaac cgtatcgctg cttgggtcat cggtacgaga
    12421 aatacacaaa aagcactgct gaaagctatg ctggaaccag ttgagcgatt gaaagaaatg
    12481 gagctggctt ttgactttac ttcaagaatg gcttatacgg aagaattgaa agatttcccg
    12541 tatgccgatg tatggaatta tttctgttac aaaaatcaag ttccagttgg tttggattgg
    12601 ctggaagaag tccaggaata tcaaaaagac gtgttggaat taagaaaata ggagtgtggc
    12661 tatattgaaa aaaatagata ttttacaagc gccttatgtg aaagaaatga tcgaaacgac
    12721 gtcgaatctt tatcgcttgg gatgggatga aagaaatggc gggaatattt cttatttact
    12781 aaaagaagaa gagatcacac cgttcttgga tcctgaaaaa gtgatccgcc gcatctcgat
    12841 gatttttgat gccagccgtt tagcaggatg ctattttatt gtaactggtt caggaagata
    12901 ttttaaaaat gtagcgagcg aacctgccga gaatcttgga atcgttcgag ttgctgaaga
    12961 tggcaaaaca ctggaacttt tgtgggggct ggaaaacgaa tgccttccta caagcgagtt
    13021 gccaagtcat ttcatgagtc atatcgcccg tttagaagta gacccagata atcggatcgt
    13081 tatgcattgc catgctagtc atctattagc gatgagtttt acgcatgaat tagacgaacg
    13141 ttcctttagt cgtacgctat ggcaaatgtg tacggaatgc ttagttgttt ttccagaagg
    13201 aatttccatc attccgtgga tggttccggg aacaaatgag attggggaag caacagctga
    13261 aaaaatgaaa gaaactcgat tggttttatg gccacaacat ggaatctacg ggtcaggaaa
    13321 agacatggat gaagtctttg gtttgatcga gacagcagaa aaggcagcag aagtctatac
    13381 gtacgtcaaa gcgcaaggac caatcttgca aacaatcacg gacgaaaacc tgtggcattt
    13441 agcagatgcg tttggtgtca ctccaaaagc aggttatttg gaggaagttc atacgaaagc
    13501 aggtgtctga catggtgaaa aaagcttttt gcatgaaagt ttacccagaa aaacaagaag
    13561 aatacaaaaa aagacatgat gaactttggc cagaaatgcg tacagcactt cgtgaacacg
    13621 gggtcatcga ctattcgatc tatcttgatc cgagtacaag tttgttattt ggttacttag
    13681 tgatcgaaga tgaaaagtta tgggaaaaaa tggcaaatac atctatcaat caaaaatggt
    13741 ggaagtacat ggaagatatc atggagacaa atccagatgc ctctccagtc agttgggaat
    13801 tgatgccagt ctttgaactg tagcaacaag caactgggca accggttgct tggggttctt
    13861 tcgtttttat tgtgaaaaat aatacaaatt aggaggaata gaaaatgagt aatcgaatga
    13921 ttttaaatga aacatcgtat tttggaaaag gagcgattga gtcgatcgtc cctgaatttc
    13981 agcagcgagg attcggcaaa gcagctgtca ttactgacaa aggattgatc gagcacggta
    14041 tagcaacaaa agtaacagaa cttctagata aagcagcaat cgcttatgct ttatacgacg
    14101 ggatcgtacc taatccaacg atccaaaatg taaaagatgg agtggctttt gttaaagagg
    14161 cagaggcaga ctgtttgatc gcaatcggtg gaggttcgcc aatcgatacg gcaaaagcca
    14221 ttgggattat tttgacgaat ccagagtttt cagacgtcat tagtttagaa ggtgttgctg
    14281 atacgaaaaa tccatgttta cctatattag ctataccaac gacttctgga acagcagcag
    14341 aagtaacgat caactacgtc atcacagatg aagtaaatca ccgaaaattt gtttgtgttg
    14401 atcctcatga tatcccaatc gtcgcgttca tcgacagcga tatgatgatg ggaatgccga
    14461 aaaaattggc tgcatcaaca ggtatggatg ctatgacgca tgctattgaa gggtttatca
    14521 caaaaggcgc ttgggaaatg actgatatgc tgcatttaaa agcaatcgaa atcatcggcc
    14581 attctttgga agcttctgta gatggcgatc aaaatgggcg agaaaaaatg gcattaggac
    14641 agtatattgc gggaatgggc ttctcaaatg tcggtttagg actggtacac gggatggctc
    14701 acccgttaag tgcatggtac aacattcctc atggcgtagc gtgtgcagca cttttgccaa
    14761 ctgtcatgaa atataacaaa gaatatactg gagaaaagta ccgtgagatc gctcttgttc
    14821 tagggattaa aggagcagct gaaatgtctt tagaagatgt aagagaagcg gcttgtggtg
    14881 agatcgatcg gttgagcaaa gcggttggaa tcccagaaac catttctgaa ttaggtgtaa
    14941 aagaagcaga tattccagca attgcagaag atgctttgcg agatgtatgc acaccaggaa
    15001 atccaagaga aacgacagta gaagaaatca ttgctttata tcaaagtttg atgtaaaaac
    15061 gaagcggatc ttcaacatta aattgcctgt aaagagaaat ggtagtttat atctg
//
LOCUS       DARBFO010000062        14911 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000062 DARBFO010000000
VERSION     DARBFO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.063"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(392..1120)
                     /locus_tag="SCK51_002018"
     CDS             complement(392..1120)
                     /locus_tag="SCK51_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEG1252636.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1098..2672)
                     /locus_tag="SCK51_002019"
     CDS             complement(1098..2672)
                     /locus_tag="SCK51_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG1252637.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2901..3839
                     /locus_tag="SCK51_002020"
     CDS             2901..3839
                     /locus_tag="SCK51_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1252638.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3851..4762
                     /locus_tag="SCK51_002021"
     CDS             3851..4762
                     /locus_tag="SCK51_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1252639.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4786..6258
                     /locus_tag="SCK51_002022"
     CDS             4786..6258
                     /locus_tag="SCK51_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1252640.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6309..6920
                     /locus_tag="SCK51_002023"
     CDS             6309..6920
                     /locus_tag="SCK51_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1252641.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6950..8863
                     /locus_tag="SCK51_002024"
     CDS             6950..8863
                     /locus_tag="SCK51_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEG1252642.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8991..11972
                     /locus_tag="SCK51_002025"
     CDS             8991..11972
                     /locus_tag="SCK51_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HEG1252643.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11969..13630
                     /locus_tag="SCK51_002026"
     CDS             11969..13630
                     /locus_tag="SCK51_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEG1252644.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13670..14602
                     /locus_tag="SCK51_002027"
     CDS             13670..14602
                     /locus_tag="SCK51_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEG1252645.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4849 a         2355 c         2996 g         4711 t
ORIGIN      
        1 gaaaactaaa aaaatatgcg gcgcacagaa cgcgccgcat atcggactaa gttttattca
       61 aatttgtctt caccaacacg atttgacgaa gagcctattt aaataactac atccatttat
      121 tctatcttct aaaatagcct ttttcatttc agttaaatag ttcagcgcca tttttgcact
      181 cgacttgcat agtagttttt tccatattcc tcaacctcag cgggggttga agacataagg
      241 aaaatcctat gcgttatgct caactggatc tgattaaata ttctaatgta tttttctcta
      301 ccatcattaa atggttaata gccaaaaatc accacttttc aatggctcct tgttgtataa
      361 gtataactag aagatgaaca cgtctgagaa attatagaag ctttttacgg taattactgg
      421 ggctgtgtcc agtagttttt ttgaagagaa cagaaaaata gctcacgtga tctattccta
      481 ctttttgact aatttcatca atagataaga atgtgtcttt cagtagagca cttgctcttt
      541 tgattcgaaa gtctagcaag taatcatgaa aggaataacc tgtttctttt ttgaaggccc
      601 gtcctaaata agcgggatgg taatggaatt gtgtagctac atctttgagc gtaagctttt
      661 cactataatt gttttgaacg taatcaatca cttgttgaat tgactcatta aaagaatcat
      721 cagttagaga gttttgatta gctgctgatt tacgcatttt ttcttcttgt actaacttaa
      781 ctaaataagg aaaatcaatg ggtttcgtta tgtaactttt aatagataaa tcaatcccct
      841 tttttgcata ctgaaaattt tggtgggcac taatgacaat actagtaaag tagcgattgg
      901 atgtttgttg caaagattcg attaattcga gtccagaaat tttccccatc gtaatatctg
      961 tgataagtaa atcaaagcgc tggttttgca ggtttttaaa tgcttcgtct ccgtctgtgt
     1021 agcagtgatc gataatgatg tcggggactt tcttcaaaag ataatgcctt atgccttcta
     1081 caattgtaca atcatcgtca acaattgcaa ttctcaacat gcatattccc ccctttttgt
     1141 attttgatag taatcgttgt tccgacgtgt agttggcttt ctataacaaa acgtgtatct
     1201 ccaggataga gatgctttat acgtcgaaaa gaatttgaga tgccgatatt ctcactataa
     1261 ggaatatcat cattaagttt ttgttgaata tgctccagct tgtcttggtc cattcctttc
     1321 ccattttcaa cgatttttaa cacataatgg ttggttgttt cagttgcata gacagtcaaa
     1381 cgattttcct tttttgatga atcgaagccg tgtttaatat aattttcaac aaatggctgt
     1441 aaagaaagtt ttggtaacat taattgatca atatgatcaa tcagctgata gtcaaagtta
     1501 tgtcgataac gacgttgatg tagttgcaag gagaggtcga tattaattaa ttcctctttc
     1561 aaatcaatta tgtcgggctg atatgtatta ttgcgataca acttgctcaa taaatatata
     1621 ctgtcgctga tttcttcgtc atcattcaat atggcagtca ttcgaataaa ttccaaatta
     1681 ttaaatagaa aatgaggctc gatttgatat tgaagtgctg catactttgc ttcttgcttc
     1741 aaccaattat tttcataaag tgttttttga tatgtagcat tatctaaata aaactgattg
     1801 atatgcatag cgatttcttt caaatcaccg actttgtttt cgatattgat tgacttgtag
     1861 tctcctgtag atgaatgatt taaggaagta atgatttcct gatactgctg gatatagtta
     1921 cgaaaacttc tatttaataa ttgatgaata gcaagaataa acaaactcat taataagaaa
     1981 atcatgattg ccagacctaa taaggtttgt gacgaataat ttggattttc atatacttgt
     2041 aatgtgtggt taccgatagc tatctgttgc gtttttttcc atttagaagg cgagatgtct
     2101 tcagataaat tgacggaatc aataccatta gacaaagaat aattgtaatg tgttggaaag
     2161 agactctttt ttagcgcgtt gatcgaaacg tctagaatca atgtagcatc gggcatgtcc
     2221 tcactaatga aacttaatga acgaataaaa gaaatttttt cgttttttgt aggtgcgtag
     2281 gcacttaagt atttttcgta atctaatgag atagatatat ttttaacttc atcatcttgc
     2341 acaagttttt ttaagttatc tactaaatag gtaggggagc gatcctttga caaaatatct
     2401 tccatgtatt cagcaggtga tgagaggtag tagtcattaa ggttttcttg gaaatcataa
     2461 ttttgataga tctctgtcac gataccggag agaatacgat tatatgtttc catatagtgg
     2521 gtgatttgct gaatgttttg caggttttga tttttttctt tttcaatcac attgatatag
     2581 gaaaagaaca atgtgaaaca ggcaagtaaa gtaataaaaa aaatcaatat gtatgtaaaa
     2641 cgcataataa cttttgataa atcgtttttc attcaaagtc ctcattttga taatctctaa
     2701 ttttatgaat cattatagca catattacaa gtgatgtaaa cgctttgcaa acagggctac
     2761 ttatgattgt gaatacagtt tccgtttaaa aactattcta ttgtagaggt ttaataaact
     2821 aaaaataatg tataaaaaat taatgtaagc gattaaccaa gtgggtataa tcaaggaaac
     2881 gctgagagag gagagacaaa atgaagctaa agaaagggtt attaggaaat ctaaaaaaaa
     2941 accgagcatt gtttattatg cttttaccag gtgcgttatg gttatttata ttttgctata
     3001 taccaatgtt tggagttgta atcgctttta aagatttcag aatttcatca aggggattct
     3061 ttacaagttt gttcaacagt ccttgggttg gctgggaaaa tttcaaatat ctctttgctt
     3121 cgcaagatgc ttggatcatt actcgcaata cggttgttta taacgttgta ggtattttgc
     3181 taagtacttt ttgtacagtg tcattagcaa tcattctgag ccaaatggcg aacaaacggt
     3241 tattgaaaaa gatgcagaca tttatgattt ttcctaattt tttatcatgg gtagtcatca
     3301 gttactttgt ttacgcattt ttgagtacgg ataaaggatt aattaatatt ttactaagca
     3361 attttggtgt ggaacccata aattggtata gtgaaccgaa gtactggcct tttattattg
     3421 tttttacgag tttatggaaa ggcgtaggct atggatccat catttattac gcgtcaatta
     3481 tggggatcga tactagttat tatgaagctg ctttagttga tggtgccagt aaatggcaac
     3541 agataaggaa tgtagtcttg ccacatttaa gatcgattat tgtcattatg ttgattttgt
     3601 ctgttggtag tatttttcga gctgattttg gattgtttta tcaattgcca agaaattcgg
     3661 gggctcttta tgatgtgacg aatgtattag atacttatgt ctaccgtgga ttaatgaata
     3721 gcggagatat cggaatgtct acggcagcgg gattatatca gtcatcagtt ggctttgtct
     3781 tgattttgat tactaataag atagtcaaca agttagatcc tagttcagca ctattttagg
     3841 aggaatcaaa atgatatcaa aattaaaacg gcagatgtac ttttcgctgc cagtcgaacg
     3901 gtttttcaca atattactaa tcttatttac tcttagctgt atttttccct ttattttcgt
     3961 aatcgtcatt tcattaacag atgaaaaagc attacaactc aacggttacg aattattgcc
     4021 ggctcaatgg agcactgatg catatcaata cttaatacaa gatggcggac agttacttcg
     4081 gtcattaggc gttacaatta tgattacagt gattggaacg ttgattactg tttttatgac
     4141 gggaacctat gcttatgttt tatcaagagc ctctttccct taccggaagt tctttacttt
     4201 ttatcttttt tttaccatgt tatttgcggg agggatggtt ccttcttact tagtgatgac
     4261 gaagatgctt ggcttaaaaa atacaatttg ggctttgatt ctacctcttg cttttagccc
     4321 atacaatgtc attattttaa ggacattctt taagaagtcg attccagaat cgattatcga
     4381 atccgctaaa atggatggct gtagtgagtt tcgagttttt ttccaaatcg ttttgccttt
     4441 agcgatccca ggggttgcaa caataggttt attttcaagc ttaggttatt ggaatgactg
     4501 gttcaacgca ttgctgtata ttgacaccaa caaattgatt ccattgcaat atttgttaat
     4561 gaagattcaa aattcaatgg aattcttagc aaataataat gacattacat tagcgcagca
     4621 acaagcaatt caaaatagcc ttcctcaaga atcaacacga atggcaatgg tcgtagtagc
     4681 tactttaccg attgcaattg tttatccgtt cttccaaaaa tactttgttc aaggattgac
     4741 gatcggtgga gtgaaagaat aaaaaagaat aggagatgtt aaaagatgaa aaagaacatg
     4801 cagaaatatt tgattggtgg tgcttctgta ctaacccttg gcctattagg tgcttgtggt
     4861 agcagtaatg gcgaagcaaa taatgaaaca ggaaccacaa aagttacttg gtatcaggtt
     4921 ggagaagaac ccaaagattt tgatgaggtc atgacaaaag tcaacgaaaa attaaaggaa
     4981 gaaatcaatg tagagttgga tatgcggctg attccggacg gggattatca gcaaaagctt
     5041 ggtgtgatga tcaattcagg agaagaatat gatatttgtt tcgtcaatgg cacagattat
     5101 gtgaattatg ggaacaaagg tgctttcatt agtctcaacg atcatttgga taaagagttg
     5161 aaagattatg cagctgaatt gaatgaagga tttattgaag gtggggcaat taatggcgaa
     5221 acttatggat tgccagtcaa taacgatatt gccacaacgg aatattggac ctttaataaa
     5281 gatttggtag atcaatacca attggatgta gagaatgttg aaggtgtaga aacattggct
     5341 ccagtattag aaaagtttgc agaaaaaaat actgatacga atattacgcc aatcggtatg
     5401 acccgtacca gtgtctgtat tccttatgac aatattatcg gtggtggtgt accgctagcg
     5461 gttgcatttg aaggcgatac acaaacagtt gttaatactt atgagactcc agaaatgatg
     5521 acttatttga aggctatgca tgagttttat aaaaaaggat atattttgaa agatgcagcc
     5581 actgttacga cagatccatt tcgaattgat agtaaaaact ggtttttagg tcgtaactca
     5641 agtggttatc aaccattagc acaaaaaaaa ttagaagctc tagcgggaca tggattaatt
     5701 ttaaaaccag ctggaaaagc ttttaaaacg actggatttg caagagtggc aatgcatgcg
     5761 gtatctgctt caagtaaaca tccggaagca gccttgaaat tgttgaatta tgtgaatacc
     5821 aatcaagaag tagcggattt aatggtttat ggaattgaag gaaaacacta tgatcgcaat
     5881 gaagatggtt cgattacttt aacaaactct gatgattatt atcaaggcgc ttgggcattg
     5941 gtaaatactt atctagtttc aaaaacttca ccgattacag aagaaagtaa aaaactagaa
     6001 gaagaaagta aaaagtttca aaatgaagca ccgatgtctc ctattttagg tttctctttt
     6061 gacgcttctg attttcgaac agaaattgca agtgtacaaa atatcgtgtc acaatatgaa
     6121 gtacttttaa gctcgggaac agttgaccct gaacaatatg taccagagtt gttagaaaaa
     6181 ctagaaaaag ctggattgga taaaattcag gcatctgctc aagaacaata cgatgaatgg
     6241 cgaaaatcta acaaataatt ttttactaaa tgaaaagaat agactaagca gttagtgggg
     6301 ggattcaaat gaacgaaaac aaatggacca aaggatttaa ttgggttttt caattgttgt
     6361 tactaaactt tacttatttt atactaattt gtttaggatt tggaatttta ggttttttcc
     6421 ctgctacgat agctttgatt gctggcgttg tgaatcgaaa aaaatatggc ttgcaagagt
     6481 ctgtgaagtt catgagtaaa atctataaga aaaattttgt tagatataat ctatattgct
     6541 ggtcagcaac tttgttgttg gccggcaata ttactacgat ctggctttgt tggcagcaaa
     6601 gcgcaccatt gttttattta ttaagtacat tattatcggt aattacactg ttcctagtct
     6661 ttgcaatgtt gaattgggtc gtgatcaaaa gtcttgggtt gtggatgatg ggagaccgac
     6721 tgattgacta tttgcttttc acagcaacga tgttgccaac ggtagtcatg caaggcttgg
     6781 tgcttggaat ttatttccga atattgatga ttttgcctgt gatttttctg tttggcggaa
     6841 cagctgtaac ggtaatattg attagtaaat taactgaaag agcgttagca aagttgcctt
     6901 gtcagataca atcgcactaa ttttttcaca aaaaggagta tttctttcta tgaagttaac
     6961 tgataaacaa gtagctaata tagcctttat ccagcaactt gagattccat tgacacaaaa
     7021 agatgaagaa caaatagact tgatttttac tgacacaaag gctaatgtgc gacaaatcaa
     7081 gattactaaa aatgagcagc aacgaatttg cttgtcttat tcacatgaag acttgctgta
     7141 tcgaggattg tttatctgcc ttccacgttt tttagctcaa ggaggagaga tgccatacat
     7201 tgaagaaacc tcgatcattg agaccagtta ttcattagat gcttctcgta atggcgtgat
     7261 gaataaagag actctaaaaa aattcatttc ttacttagcg ttgatggggt acaacacatt
     7321 gtatatttac acagaagata cctatgaagt agaagacctt ccatattttg gttaccttcg
     7381 cggagcgtat acaaaagaag aagtgaatga aattgttgaa tttgcttctg gttttggcat
     7441 agaagtagtt ccatcgatcc agacccttgc tcacttgacc caatttttga agtggtatgc
     7501 tcaacaagaa ctgatggatg atcaaaatac tctactgatc ggtgaagaaa aggtttatgc
     7561 ggcaatcgaa aagatgatta ttgcctgcaa agaaatgtat cagacagagc gtattcattt
     7621 gggaatggat gaagcctatc aagcaggtct gggacgatat ttaagcctgt atgggtattg
     7681 tgatcggaca actttaatga ttcaacatat ggaccgtgtg ttatcaattg ttgataaata
     7741 taagatgaaa ccaatgattt ggagtgattt catttataag ctattagatg aaaccggtaa
     7801 agataggtat tacaatcctg attttaaact caaagcatta gcagctaaag gattaccagc
     7861 aggattaacc tatgttcatt gggactatgg ttcagatgaa gtttcctcct atgagaaagt
     7921 aattgataac catttgaaat tttgtgacat tgatcattac gttttagcta gtggtgccca
     7981 tatctggggg agactagccc cgaatcatgg gaaaacagaa gcaattgttt ctgcagctgt
     8041 aaaagcttct aaaaataaag gtgtacgttc tgtgatgtta actacttggg gagatgaagg
     8101 gcaggaaaca gagcattggc attccttaat tggggcatta aaattagcag attatgtcta
     8161 ttctgaagaa gagtggaatg attttgagga aatctacgat cgtttattag gaacaggtag
     8221 ttatcaatta atgtccgaat tacgatattt cgatgaagtg gataccatta caaataacaa
     8281 cttacaaatg gcttccgtga gtaaactgct actttggcaa gatccgttag ttggtatata
     8341 tgactttcat gtagccgctc ataacaaaat tgccactaga acaattggta cttattatca
     8401 tgagttagct cagaaattag atatgaataa aaattggaat ccaaaacaac caattttaac
     8461 aactattcgt caacgatata tacttttagc tgaaatttta gcacaaaagg cagaactagg
     8521 catctattta caacactgct gtctggtaga agaccttgca ggattaatgg aattagcgaa
     8581 tcggtgtgaa gcactagcca atcgagtaaa tgaattggca tctattcatg aaataatttg
     8641 gtatcaaaca tacaaaacct atggttggga agtattagaa caacgatatg ctgcattagc
     8701 tcatcgtctg aaaaccgttg cgcgtcgtat taaaaatcat cttcaggggc aagatgccct
     8761 agaggagctt ttggaagaac gattaccgtt ttgtcaatat caagtgccgc ttgaaatttc
     8821 tggctttaat tatcggcaaa gctcggtgtc aggctacaat taacgaagga atcttcccgt
     8881 tgaacggacc acttgctcat ctaatgggaa agtggttcgt tttctattca taagtagctt
     8941 accactaact cttttattaa gaagagaaaa aaagtaggaa gtgagaaaat atgttatcgg
     9001 actattatca aaatctgtca gttataagtg tcaatacaga accattacgt aattattata
     9061 ttccttatgc aagtaaagaa gaagcaaaac aagagttaga aagggaaaaa acgagccagt
     9121 ttatcgatct caatggtaca tgggattttc agtattatcc ttctgtccaa cagttaccgg
     9181 aaaatttttt tcaagaggcc tttactgaaa gctatgatag aatttctgtt cctggagttt
     9241 ggcaaacaga gggatttgac ttacctcagt atatcaatgt atcttatcca attccttttg
     9301 atccgcctta tgtacctaat gatacccctt gtggtgtata tcggcgtcag tttcaaattg
     9361 agcaaacaga tgcacgcttt tacttaaatc tagaaggagc agatagtaat tgttttgttt
     9421 ggataaatag aaactttgta ggctatgacc aagtgagtca tagtacatct gaatttgatg
     9481 tgaccaagta tttacaggtt ggggttaatg aaattcaaat tctagtgatg aagtggtgtg
     9541 atggtaccta ctttgaagat caggataagt ggcgaatgag cggtctattt cgtccagtgt
     9601 atcttcttca acgaccaact atgcatctga caagttatca actacggacg ttgactgatc
     9661 ctaaaaaagg aagcggaaca ttaatggttt cttttcaacg atttactcct attgaagtat
     9721 cgttacctat ttcttacgaa ttattccatc ctgatggacg tctcatagcg aaaggggagt
     9781 gttactcgga acaacaaata aatttggatg ttgaagattg ccttttttgg acagcagaaa
     9841 cgcctaaatt atatacatta cttttaacat gtaatggtga atattttaaa gaaaaagttg
     9901 gctttcgagc actgacgatc aagaatcgta agttattgct gaacggaaca cctattaaat
     9961 tatatggagt caactatcat gacgtgaatc agcaaggcac gccagtgatg acccgagagc
    10021 agttacgtgc agatttgtta ttaatgaaaa aattaaatgt gaatactgta cgaacggcac
    10081 attaccctaa atcaccagat ttttatgaat tggcggatga gattggtctg tatgtaatca
    10141 atgaagcaga tttggagtgt catggtgtgg ttaatctata tgatactgaa gcgaattatc
    10201 aactaattgc taatgatccg acattcgaaa agccgattat ggaccgagtg atccgttcta
    10261 ttcttcctta tcaaaatcga agctctatca ttatgtggtc tatggggaat gaatctggtt
    10321 atggagtaaa ttttgaaaaa gctcaagctt tagcacggaa attggatgat tcacggttga
    10381 ttcataatga acgctcgatt gaaccagtta ttgaccaagt gaatgatttt agtaacttgg
    10441 atgttattag tcaaatgtat cccccactgg aagatgttca tcgtcattgt caaagaagtg
    10501 atactgaaaa accttttttt atgtgtgaat atagccacgc tatgggcaac ggtcctggag
    10561 atcttaaaac gtacttcgat ttgattcaac agtatgattc ctttcttggc ggttgtgtct
    10621 gggagtggac tgatcatgca atcaatagag gaactttaga aacgccatct tatttatacg
    10681 gtggtgattt tggtgaaaaa tatcacgacg gcaatttttg cgtagatggt ttattgaccc
    10741 cagatcggca attaacagta ggtgcattgg agtaccgtaa tattcattcg cctattcaac
    10801 tgattgctgt agatgaggag ctgcaagaag taaccttgag caatataact gcttttacag
    10861 ccggttcagc atttaacctt tctcttgaat ggtatataga tggaaaaaaa acagtttatg
    10921 aagtaatgaa tcctttcata cttgagcctg gcaaaagttg ttcattgccc atcacagtac
    10981 ctacaacgat tcatggtgct catacggttg ctgtttcgct atctgtttat aaaaatgatg
    11041 agctttatgg acagcaacag ctctttctta aaaaagaaaa aatgagattg ccaagaactg
    11101 ctcatcataa tgatgcacca caaatttcag taaaaggcac agagctgata atcaaaggag
    11161 aatcctttat ttatatatat caattagcaa aagctgcgtt cacttctgta aagatcaatc
    11221 aaaaggaact tttggaacgt cctactcaat tttcaatttg gcgtgcacca acggataatg
    11281 atcgtaactt agtaaacaaa tggaaagcag ctggatatgc tgaaatgtct ttagaaatca
    11341 gaaaaaaaga ctggaaattc gtagatgatc atctcctgat cgaaacgtct tatcgaggag
    11401 ttattccagg attacagcca atttttacaa tgcacataca gttgaaaata aatggtgcag
    11461 gcgtaatcaa ccaaaaagta tcagtggaga aaaatccgat ttttccagat ttccctcgct
    11521 ttgggatgga ctggccgttg aatgatactt tttcgaaggt ttcttattgt ggctatgggc
    11581 cgtatgaaag ttatcctgat aagcactatg ctgcgatgtt tggtatttat gagatggaga
    11641 ttgaggaatt tgatcagcca tatatcaaac cacaagaata tggtaaccgc tggcaatgtg
    11701 attgggtatc aatcaaaaat catcaaggat ctaccatgac ggtttatgga aactctaatt
    11761 tttctgctct gccttatagt caagctcaat taaccgaaaa gtcacaccat tatgaattaa
    11821 taaaagaaaa tagaacttgg ttcaatttaa gttataaaca aaatggtatc ggctcacaca
    11881 gttgcggtcc ttatttagaa gatcgtttcc gttttatgga agatactttt gactggaata
    11941 tagtgattac tttcaaagga gaaaatagat gaaagaaaca aagatcaatc aagtcaaaaa
    12001 aaaattatct cgttatcaag aagttgttta tcgaaaccat tgcttaaaat ggtcaaaaaa
    12061 taagctgaat gttcgtttta atggattatt ttatcgtttc gaacagttag aagaagtctg
    12121 gaaaccggta acgaatttcc aatcatgtcc tgtacagctc ataaatgatc caaaagaaac
    12181 atacgattta tgtataacac acctttataa ccagcatttg aaaaatgacg gattttattt
    12241 aaaggttact aatgacaatc aacttacgat tgaatcagca aaccttcgag ggtttcaata
    12301 tgctatggaa gccttattag agagtttttt ttcggtagga gatcaattat tattaccgga
    12361 attgattctg aaacatgaac catcagtgaa aattcgagga atcattgaag ggttttatgg
    12421 tatcccctgg acacatgaaa tgcgcctctc tcttttttca tatatgaaag ataaccaatt
    12481 gaatactttt atgtatgccc ctaaagatga tgaattatta cgtaaaaaat ggcgtgaact
    12541 ctacgatgcg caagaattag acaaatttaa ggagctttta tctgctgctg aggctagcaa
    12601 tatcgatttt tggtacttaa ttagtcctgg aaatgatatt gatattacgt gtgaagaaga
    12661 cattcaagtt cttctgaaaa agttagaaca actgattgag cttggcgtat ttcaatttgg
    12721 tctattaatg gatgatattg actatcaatt aaaaggtgct gctaaacgcc gttttcgaga
    12781 gccagcattc gctcatgcct atttagtgaa tcgagtagag gaatatctta gcactcggtt
    12841 gtccaatcat gaattagtca tttgtcctac agaatatgat aatcgtcatg gttctcgcta
    12901 tttagcaacg ttaagtcaag aaataccaga acaattaccg ttcttttgga ctggcccaag
    12961 cacgttagcg gcgtcaattt ctacagaaga gctgcaggaa atggcttctg tctatcaacg
    13021 gccgatgctg atttgggata atattccggt aaatgattat ttagaggata aagaactatt
    13081 gttcatgagt ccatatgaga atcgaacacc gaatctttct aaggagagat accaggttac
    13141 aggagtagtt tcgaatccaa tggctcaatt agaagcttca aagtttacga tcaatagtat
    13201 ggctaattac ttatggaact gtgagcgttt tgatcctttg gaaacttgga cgtcagtcgt
    13261 tgaaaaagta gttggaagta aactacaacc agcttatctt accttaacca atgcttttcc
    13321 aaatcattac acaagcagtc tgctttcaga tgaagaatta ctgctagcca aagacccaga
    13381 atgggtcaca aaagaaatgg ctgctttagt ccaagcagta aaatggatcc agcaagaaca
    13441 acatacatct agattgagaa cagaattgga gccttggtta caagcaatca cagaaagttg
    13501 gactttctgg caagaatggc aattaaaaaa agatccaaaa gaagcagaag aatggttggc
    13561 taagaaaaaa acacaccgca taggacgtga tttagtaact gcccatttag agcaaattat
    13621 caaaagttag atatcttaaa aaataaaaag caagaaaaag gagaacaaaa tggtcaagag
    13681 attaattagt gcaaactaca gtgatgtcaa gaaaatgacg tcaaaagagt tgaagcaatc
    13741 catcaaagcg agtgaaggac gggtgattgt ttgtgaaacg gttgctaata ctcttccaca
    13801 atctggagaa attacgaatg cagaagttgc gtcagcattt ggtgcagatt tgattctttt
    13861 aaatggtttc gattgttatc gtcctgcagt ttttggacta ccaaatgtga cgattgagaa
    13921 ggtttttgat ctacagcaaa cgattgaaaa cccgattcct aaattaaaag aactagtcgg
    13981 tcgtcctgtg ggaattaatt tagaacccat tgcccctaaa acaaatatga tgagtgagaa
    14041 agtagaaatc agccaaggaa gagcaagctc tgttgaaacg atacaaaaag cagaagaact
    14101 aggagtcgat tttatttgct ttaccggtaa tccaggcact ggagtaacca atttagaaat
    14161 tactaaatcc gtaaaaatgg caaaaaagta tttttcggga ttgattattg ctgggaaaat
    14221 gcatagcgcc ggctcagaag agccagttgt gaataaagag gcgattattt cttatgcgga
    14281 agcaggcgca gatattttac tagttccttc agtagggtct atcccaggct ttttagagca
    14341 agacctaatt gaagcggttc ggttggcaaa aaaatatgat ttgttaacga tgtctgcaat
    14401 cggtactagt caggaaagtg cccgaccaga aacattgcgg caattagcca ttatgaataa
    14461 aagttgtggt gttgatttac aacatattgg agatgctgga tacgggggat tggcaccagc
    14521 agaaaatatt ttcgaaacct cggttgcaat tagaggaatg cgacatacca tcaactgcgt
    14581 tagccgctcg atcaatcgct aacaaaatta aataaaaaat ggttaaattg attatttaag
    14641 tgggaacata cttgagatat cagctaactt ctgtcacttt aaaaaaggga ctgtgacaga
    14701 agtgaaaata gacaaaaaat atccgaacaa gagactagga tagcagaaaa actatcgttt
    14761 tgcaacgagg accaactgga ccgtaggagc aattctctta ttgttcggat atttcgcttg
    14821 ttcaaggact tatgtctgta gtgccctttt ttggtaggaa aaatggtagg gaaccatatc
    14881 atttttaatg aaaatcaagc atttcaaaag t
//
LOCUS       DARBFO010000063        14717 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000063 DARBFO010000000
VERSION     DARBFO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.064"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(191..757)
                     /locus_tag="SCK51_002028"
     CDS             complement(191..757)
                     /locus_tag="SCK51_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1252646.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(757..1713)
                     /locus_tag="SCK51_002029"
     CDS             complement(757..1713)
                     /locus_tag="SCK51_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEG1252647.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1713..2369)
                     /locus_tag="SCK51_002030"
     CDS             complement(1713..2369)
                     /locus_tag="SCK51_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEG1252648.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2739..4757
                     /locus_tag="SCK51_002031"
     CDS             2739..4757
                     /locus_tag="SCK51_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1252649.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4827..5033
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5184..7598
                     /locus_tag="SCK51_002032"
     CDS             5184..7598
                     /locus_tag="SCK51_002032"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEG1252650.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7946..9499
                     /locus_tag="SCK51_002033"
     CDS             7946..9499
                     /locus_tag="SCK51_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEG1252651.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9880..11664)
                     /locus_tag="SCK51_002034"
     CDS             complement(9880..11664)
                     /locus_tag="SCK51_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1252652.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11679..13400)
                     /locus_tag="SCK51_002035"
     CDS             complement(11679..13400)
                     /locus_tag="SCK51_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1252653.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13500..14261
                     /locus_tag="SCK51_002036"
     CDS             13500..14261
                     /locus_tag="SCK51_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1252654.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4783 a         2718 c         2925 g         4291 t
ORIGIN      
        1 agctgtcctg catcaagtaa taagaacagc caatcaaaaa cagcttctca tgtttttgat
       61 ttggttggac ttattaccgc agatataagc ttttgagcac cgtgaataaa gaggttatga
      121 aatcaacgct ctaacctgag tattagagaa aaaaacgcaa aacatatcat ttatgttttg
      181 cgttttttat ttatcttttt ttctcaatac aatataaaat aggcggctgg ttcttttggt
      241 tgataaaccg ataattcagc acattatatg tttcctgtgg cagtttggaa caaaaagaat
      301 caaccatatc cagttctttt tctccacctt catgaccata gtagacgaca aggatcaatc
      361 gtccgcgagg cacaagtcgt ttcaatattt cttccatagc tgtgcgtgta gtctctggca
      421 tagtgatgat tgctttatca ctttttggga gataccctag attgaaaatc cctgctttga
      481 gattttgttg ttcagctatc acttctccta acgtttcgtg gccttgtaga aacaatgaga
      541 cacgttcttc taactgacgc tgttctagcc tttcacgagt agcatcgatt gcttgttgct
      601 ggatatcaaa actatagaca tgaccagttt ttccgatgtg ttctgctaga aaaaccgtat
      661 cgtggccatt gcccatcgtt gcatctacga cgtgatcccc tggttgcaag atttcttgca
      721 gtaatgtatg gctgaaatgc aaagcagttt gcaacatcag acgctcacct ttttccgtac
      781 atcatatttt ccttggaagc tatctctgcg aagaagttct tcatcaattg cattcaagac
      841 ttcccatttt ttcaagctcc acattggtcc aatcaaggaa tcccacggcg catcccctgt
      901 caaacgatgg atgatgatct cttttggtat catttccagc tggtcgcaaa tgacattgac
      961 atagtcagga cggctcatta attgaaggcg accttcatga tagtcccgca acattctcgt
     1021 tttgcgcatc agatgcatca aatggagctt gattccttgg atatctgaat ctaaaatggt
     1081 gcgtctgaca ttttctttca tcatttctaa actttcgcct ggtaatccgt tgatcaaatg
     1141 cgtacaaaca cggatattat gtttgcgtaa tttagccacg ccatctaaat aagtttgata
     1201 atcatgtgct cggttgatca gttttgaagt ttcttcaaaa gttgtttgca atccaagttc
     1261 tacccataaa tacatccgct cgttcagttc agcaaggtaa tcaactactt catctggcag
     1321 acaatccggt cgtgtaccga ctgataatcc tacgacacct ggtaaattga ctacttgctc
     1381 aaatcgttct ttgattattt caactggcgc atgggtgttt gtaaaatttt ggaaataaac
     1441 gatgtattgt tggacatgag gccatttttg atgcatcatg tcgatttctt tctggaattg
     1501 gattggaaga gggtcttcag gtgcaacgat catgtctccc gaaccagata cactgcaaaa
     1561 agtacaaccg ccatgggcaa cagtcccatc tcgattcgga cagtcaaatc ctccatcgat
     1621 cggcaccttg aaaattttcc cgccaaactc atttcgtaaa gcgtaattcc aagaatgata
     1681 tcttttattt ccttctgaat aaggaaaagt cattaaaagt ccttctttct gtaagagtta
     1741 tctatttgtt ttcttttttc tctatacact ggataagtca ggcacagcca agaaagactg
     1801 agtaagtaac cgccgatgat atcagtagga aaatgaacgc ccagataaat gcggcttatc
     1861 ccgacactta ctataataaa catcatcagc aattgaagca atgtgcgtaa ggttcttttt
     1921 tgaacaaaag ctggaagcag aaatagtaaa gaaccgtaaa gaatcatact gcctgctgca
     1981 tgtccactag ggaaactgta acttgtttca actactaagt gcgccaatga aggacgctct
     2041 cgcatgaaaa atagtttgat caccgggttg atgattccag caattacaat aaatccagaa
     2101 acgaaccaaa atgtttctgc cctttttttc ttataccaca tccataaagc cgcacaagca
     2161 aataaaatga tgacggtaag aggattaccg aatttagtca taaagagttg gtagctgttc
     2221 agatcaggat aagaagctct gacaatgcct gtaaaccact gatcgaaacc agaaagccac
     2281 gacagatgga actttacgat ccagcccaaa atgaaaaaca ggacaagaaa gaagctacca
     2341 gccacctgaa atgatgtttt atatttcata caattcctcc tttgaagcaa tgacttatta
     2401 tagcattttt ggtccagcga aaaaaacggt aaagagataa aaccaatgta tgattcctct
     2461 ttgaataacc aatattctta taaaaaaaat aactgtaatc ggaaagacat tttttggtaa
     2521 tgcaaatgaa atgttaggga aataaaatca gtgtttttcg aaatcctttt aaacaagaac
     2581 gtagacgtag gaagaacttg aaaatgaaca gaagttgttc attacggtaa attacaatat
     2641 gattacaaaa aacaacaaag ctcttaaata acttttaata aatattaaga gaagttataa
     2701 ttattaataa tagataaatt gaagttggag gatagaagat ggaaaacatt gcaagaaaag
     2761 aacggagacg tttagaacag accaaacgtt ttcgaaaagc aaaacgaggg gcagcaatcg
     2821 tgggaactgc gatggtaggc tgttctgtcg ctgccccatt agtacagccg gttcctgttc
     2881 aagcggatga aacacctgtt cagttcggcg caagaatcaa tacagcggca tttatcacag
     2941 aaattgctac ttatgctcaa ccaatcgctc aagccaacga tctatatgca tcagtgatga
     3001 tcgcacaagc agttgtagaa agcggttggg gcagtagtgc gttatctcaa gcaccttatt
     3061 ataatctttt tgggataaaa ggcagctatc aaggacaaac cgtctacatg gatacgcttg
     3121 aatttttaaa taatcaatgg gtaaataaaa aggagccttt ccgacaatat ccttcttttg
     3181 ctgaatcttt caatgacaat gcctatgtac ttagaaatac ttcatttggc aacggttatt
     3241 actatgccgg tacttggaag agcaacacga aatcctatac agatgcgaca gcttgtttga
     3301 cagggcgtta tgcgactgat cctggttacg caggcaaatt aaataatatc atcgcaactt
     3361 acggattgac ccaatatgat acaccagcta gcggaaacgc aggcggtgga gcgactacag
     3421 gtggcaataa tactggaagt gcaggtagta ctggcggttc aagcaattct ggtaatacaa
     3481 actcaggttc agcaacttcg accacttata ctgtccaatc tggtgattct gtttggggaa
     3541 tctcaaataa attcggtatc acaatggatc aattgatcca atggaacaac atccaaaaca
     3601 atttcattta tccaggacaa aaactgacaa tcaaaggtag ccaggaaaat ggctcttcga
     3661 cgaataattc cggtaacaat accaattctt caggaaatgc tggtacatca aacggaggac
     3721 aaacaactgg tgcgaaatac actgttcaat ctggtgattc tgtttggaag atctcaaatg
     3781 atcacggaat tacgatggct caattgattg aatggaacaa tatcaagaac aatttcgttt
     3841 atccaggaca acagctaatc gtatcaaatg gcggttcttc taatgctgga gcaaatactg
     3901 gctcaaatac tgggaataca agtggctcta gttcgtccag ctcaagttct tctaatacag
     3961 cttcaggaac gaaatataca gttaaagccg gagactctgt ttggagcgtc tcaaataaat
     4021 acggaattac gatggctcag ctaatccaat ggaacaatat ccaaaataac tttatctacc
     4081 caggacaaca actgactgtt tcaaacagtg gatcaacaaa cagcacgtca ggtaatacag
     4141 gttcgtcaac caatacaaat aacagcagtt cagttaatac tggcagcagt tcgtctcaag
     4201 gcacttatac cgtgaaagca ggtgaatctg tttggagcgt ggcaaataaa aacggcatca
     4261 cgatggatca gttaatcgaa tggaataata tcaaaaacaa cttcatttat ccaggacagc
     4321 aattgatcgt taaaggtgga acttcggcaa atacaaatac aggttcaact acttctgcag
     4381 cgaaaccaaa tacaccgaat acgtctgcta cgacaagtac atcatccact ggtaatacga
     4441 tgtataccgt gaaagcgggc gagtcagttt ggagcgtagc gaacaaacat catatcacga
     4501 tggatcagtt aatcgaatgg aataatatca aaaataattt catctatcca ggacagcaag
     4561 tgattgtgaa gaaaggttca tctcaatcaa caaaccaaca atcagttact ggttcgaaaa
     4621 cctatactgt gaaatctgga gaatctgttt ggagtgttgc tgacagtcat ggaattacaa
     4681 tggctcaact aatcgaatgg aacaatatta agaacaactt tatttatcca ggtcaaacgt
     4741 tgattgtagc taaataaaat agctgttcga agaaataacg attgaattcc acaaaaatat
     4801 ttgttactat aaggctactt aaatatgaca acacttgaaa aggaagtagt aaaaagactg
     4861 aagtttcaga gagcgcatgg ctggtgagaa tgtgcacgga tgcttttgaa ctcgcctttg
     4921 agttgccctt gctgaatacc cgagtaagca agcggcggtg acggccgtta caacgttcga
     4981 ggccttttca ctaaggtaaa attaggtggt accgcgtaga ttacgtccta taaaaagagc
     5041 aatcttttta taggattttt ttatttcttt tgtgaagttt gagaaaacaa agcaaaagga
     5101 aataaaaata aacagattct gccggttttt tcgttatatt tataatagaa acaagtgttt
     5161 tcaatagaac aggaggacag caaatgagtt acaatcataa agaaattgag aaaaaatggc
     5221 aaaaatactg ggcaaaaacg aatactttca acacccatga tgatcctgaa aagccaaaat
     5281 tttacgcact ggatatgttt ccctatccat ccggtcaagg gctacatgta gggcatcctg
     5341 aaggttatac ggcaacggat atcctttcac gtgtgaaaag aagtcaagga tacaacgtgc
     5401 tgcatccaat ggggtgggat gcattcggtc tgcctgctga acaatatgcg ctagatacag
     5461 gaaacgatcc agctgagttt acccaaaaaa atatcgaaac attccgacgc caaatcaatt
     5521 ctttaggatt tagttatgac tggaatcgcg aagtgaatac aactgatccg gagtattata
     5581 aatggacaca atggatcttt acgaagcttt atgaaaaagg tctagcttat gaagcagaag
     5641 tagcggtaaa ctgggtacct gaattaggaa cagttatttc aaatgaagaa gtgatcgatg
     5701 ggaaaagtga acgtggtggc tatgatgtag tcagaaaacc aatgcgccaa tggatgctga
     5761 aaatcactgc ttatgctgat cgcttattgg atgatctaga attagttgat tggccagaaa
     5821 gcattaaaga aatgcaaaga aattggatcg gtcgttcagt aggagcaaat attgaattta
     5881 aagtggctgg tacggataaa tcctataccg tatttacgac acgaccagat actctattcg
     5941 gtgcaactta ctcggttctt gcacctgaac tagatcttgt tagagaaatt acgacccctg
     6001 aacaaaaagc tgctgtagaa gcttatatcg aagagacagc taaaaaatca gatctaaaga
     6061 gaacggatct tgcaaaagaa aaaacaggag tatttactgg tgcttatgca atcaatccag
     6121 ttaatggcaa ggaaatccca atctggatcg ccgattatgt attatcttca tacggcacag
     6181 gagccatcat ggctgttcca gcacatgatg aacgtgactt tgaattcgct caaactttcg
     6241 gactagaaat tcttccagtt atcgaaggtg gagatgttga aaaagctgct tacacagaag
     6301 atggtacgca catcaactct gaatttctag acggcatgaa caaacaagaa gcaatcgata
     6361 aaatgaatga atggctagaa gaaaatggcg tagggaaaaa agaagtcagc tatcgcttac
     6421 gtgactggct attctcccgt caacgttact ggggtgaacc aatcccaatc atccattggg
     6481 aggacggaac agtcacagct gtcccagaag aagagctacc attacggttg cctaagacaa
     6541 aaaacatcaa gccaagcggt actggagaat ctccattagc aaatatcgag gagtgggtca
     6601 atgtcgttga tcctgtaaca ggtaaaaaag gccgtcgcga aacgaataca atgccacaat
     6661 gggcaggaag ctcatggtat tacctgcgtt atatcgaccc tcataacaaa aaagaactag
     6721 cagattatga aaagttgaag cgttggctac cagttgacat ctacattggt ggagcagaac
     6781 atgcggtact tcatttacta tatgcacgat tctggcataa attcttatac gatatcggtg
     6841 ttgttcctac aaaagaacca ttccaaaaac tatacaatca aggaatgatc ttaggcgaaa
     6901 acaacgaaaa aatgtcaaaa tctcgtggta atgtagtcaa tcctgatgat gtggtcgaaa
     6961 cttacggtgc agatacactg cgcatgtacg aaatgttcat ggggccatta gatgcttcga
     7021 ttgcttggag cgaaaatggc ttagaaggaa gccgtaaatt cttggatcgt gtatggcgtt
     7081 tgatcgttga tgagaacaat aaaatgcgtg accgtatcac tacattgaat gatggcaagc
     7141 ttgataaagt gtatcatcaa acagtgaaaa aagtgacaga agactatgaa aatcttcatt
     7201 tcaatacagc aatctcccaa ttgatggtct ttatcaatga agcctataaa gtggacgctc
     7261 tgccttatga atatatcgaa ggatttgttc aactactcgc accgattgct cctcacatgg
     7321 gtgaagaact atgggcaatc cttggaaatg atggtggtat ttcttacgca ccatggccaa
     7381 catacgatga agcagcactt gttgaagacg aagtagaagt tgttttccaa gtaaacggta
     7441 aagttcgtgc gaaaagcaat gttccccgag acttaggaaa agatgagcta gaaaaagcag
     7501 cattagccaa tgaaatagtg caagaatata ttgaaggcaa aacagtcaga aaagttattg
     7561 cggtaccaaa caagttagtc aatatcgttg ctaattaatt ggctctttgt caataaggac
     7621 tgatgagttg tgcaaaatca aatctgggcc agaatgaact tcattctgtc tcagattttt
     7681 tgttatttca aattgattaa ttgattgatc aaaaaggttt tgattttcat gttctcaaac
     7741 acgttcaatg tctttttctt ctgaatggtc ttgtaagaga tctctgtaat catcaaattt
     7801 ttgtctatta atcttagtat tttttgatgt ataaactacc tgaaattgag agtacatacc
     7861 aaattcataa attttttatt agtgggacta gtgattgtat ggattttgac gcataatgaa
     7921 tgaagtatga gaaaaggaga gacaaatgga tcaagaacta tttattatcg aagtattatc
     7981 agctaaacaa aaaattatcg agaaagcgaa aaaaagagcg aaaagaataa tgttcaagct
     8041 tttaagctta gtatttttct ctggtttgct ttttttcgga gctgtatcgt tcctatatcg
     8101 ccatatgagt gcagtagtcc gttcagctga agggatgcaa aaattaaata atttgtatca
     8161 cttttcgtgg ggagttggcg gaataacaat tgttgtgctt attgtttttc ttctacttat
     8221 ttggctccgt taccatcaat tgtcgcgtca tttcgatcaa tggcttagtc aaagagcaca
     8281 acagttttta cataataaac caacttctca agatgatgac tatctttatt ttgagaagac
     8341 ttcgacgagt caagcttttc ggatacaaaa aagtgattgc cagttattgc tgagatcact
     8401 tgatgattta cctatctact taggcgaaag caaatcaaaa ttcggaatga acacattcca
     8461 tattttttta ctagaaaacg aacccagatg ggaaaaaagc cagtcttcgc ctggtggata
     8521 cctgacaact aagatgagta cattagtcac tggttttttc ctcattcttt tagctggaac
     8581 aatctattat gggagataca ctcagccaaa aatgtcttct catataaatg ctgcttctca
     8641 catgacaatg aactcaacta acgatacgca tatacaaaag acaaatgaag ggttgttagc
     8701 tgatcctaag caaaaggtaa atttgacaac acaaaagata aatgatctca aacttgatac
     8761 agagacgcac tctctctata tgacaacaga tagtggtgct aattgggtat ttgtgccgat
     8821 tgattcgcaa tggttgcgtt ttggcgaata caccctgaca acggggctga ttcctgaagg
     8881 ttattggatg gacaacacat atgatatttc cgccgatttc tcatggttta tctattcttc
     8941 tggggatgat gtacgtctgc tgacttcaag agataatggt aaaacatggc aaacgtctat
     9001 tatcacccaa tcagttcagc cgatacgtta tcgaaaagcg gtattttatg gcaacgatgg
     9061 tctatttatc tgtacatcag ccagtgggat gtctgcagaa actttgtaca tttacactac
     9121 tatggatggc ggaatcactt ggataaaaag tggaagtaca atgatcaatc agccagtaca
     9181 aaatgtcagc ttcgttacaa cttctttagg ttttgtttcg acacgtgaaa gtttgtttta
     9241 tacaaacaac ggaggacgct cattcaaaga atcagttctg tcgattccgg aaaactatca
     9301 gacagggggg atagatcttt ttcaatcacc aaatgaagtt actcaaataa gcgcgaatac
     9361 gttagagaca aaattttatt tgttgaaaaa aggaccaatc gatgcaggga aaatgtttgc
     9421 ctgcctcttc cgttcaactg ataacgggga aacatggcaa ttcgttcagc aactatcgca
     9481 agtagaacaa ggagattaaa gaagagagat tgtgagcctg acgttcagca ccgagtcaca
     9541 agaaacaaat tcaaaaaaca gcttctcata tttgttgaat agtgtaactc gtgattcgga
     9601 gttgacaaga gcaacaagaa gcgattttga agtatagctt ctcgtatttt acaagatcgt
     9661 gtcttattgc cgataggttg tctcttgaac accgtttatt cgtaaaaagg gattgtgaca
     9721 agtcttttgt cactacttac cgaacgaaac tgaaggagct atccaaaaag tatcattagt
     9781 attattcttt gtgtttctag tggcaccact agtacattta aaaaagctat cgatcatgga
     9841 ctaatcatac atgatcgata gctttttaga taatgtttgt tacgctatct gaaattgact
     9901 attataaaga ctggcataaa gcgttggtga ttggagtagt tcagaatgtg ttcctttttc
     9961 gacgatatcg ccattcttca ttacaaggat catgtctgca ttttcaatcg ttgacaaacg
    10021 gtgtgcaatc acaaaacttg tcctatcttt tgtcacagct tccattgctt gctgaatcaa
    10081 tgcttctgtt cgtgtatcta cgctagaagt tgcttcatct aaaataacga caggaggatt
    10141 cgctagaatg actcgagcaa ttgtcaataa ttgttgctgt ccttgagata acgtgccgtt
    10201 ttcgctagaa atgatcgtat catatccgtc tggcagtgtt cgaataaagt gatcacattg
    10261 tgcgatttca gccgcttcga taatttgttc tcttgtggca tccattttcc cgtaggcgat
    10321 attatcagct accgttcctt caaacagcca ggtattttgt aaaaccatcc caaaagaacg
    10381 acgtagttct ccacgtgaaa gcttggtaat atcaagaccg tcgaattcga tctttccttt
    10441 attcagttca taaaaacgca tcagcagatt gactaaagtc gtttttcctg caccagttgg
    10501 accaacgatc gctaccgttt gttttggctt tactgaaaaa tcgacttgat tcatcaatat
    10561 tttatctgat gtgtaaccaa actgtacatt tttgaaagct atttttcctt tcggttgttc
    10621 taaaagtctt gcatctggaa ggtctggaat ttcatctgtt tcatctaata tctcaaatat
    10681 cctatcgatt gctgccattg cggattggat cgagttgatc acataagaag cggtagaaat
    10741 tggttcagaa atctgattga tgtactgaag ataagcttgt aagaagccga tcgtcacacc
    10801 acctgataga acaagcatag caccgaatac cgcacttatg atgaaggcga gttgattaat
    10861 gaaacgaatg gccggataga tagcacagtt cataaattga gcacgtttaa aagcgttgta
    10921 ttgcttttta tttacttcgt gtatagcagc ttctgcatga gcttgctgat tgaatgtttt
    10981 gaccactaga ttaccagcta agatttcttc cattcgatta tttaaaacac ccagttcata
    11041 ctgattctgt tcagaaatct gtttgttttt gttcgcaatc ttagttgtaa caattgaact
    11101 gccaacgatc agcaataaga cgagcaaagt aagcttgctt tcgatataaa acagcatcag
    11161 accagcagca gctattgtta cgacagaagt aaaaaattga ttgatacctg tcaataacac
    11221 ttgagaaagt tgattgagac cggtagttgt ccgactaatg atgtcaccta cttgatgatt
    11281 gtcaaaaaac gacatcggta atgttttgaa tttttttgtg atttcttttc gcaccctcaa
    11341 tgtgatcttt tcgcttaacg ctgccattgt ccgttcctga ataaatgaag tgatgctgct
    11401 taagactgaa cagccgatca gcaataagac agggaacaac aatgcttgtt tgatatcact
    11461 gaaagaaagc tgtccaatac ctgtttttct tatttctgat aagagatcat cgatcccgat
    11521 tcccatgatc aagggcatgg ctacgactaa taggttcccg aataaactgc atagaaacaa
    11581 tccgtaaaaa tatttatgtt caggtttgat cattttccaa aaacgtatca aagcacgcgt
    11641 gttcaacgtt tttttctttt ccatcgtatc ccttctttct atattccttg tgattttgca
    11701 aactcctgat aagtttggca attagacagt agttcttcat gagtaccttg tccaacgatt
    11761 tttccttcat ctaaaacaat gatgttatct gcttgttgga ttgtactgag gcgttgagca
    11821 acaatcacga gagttttctc cgacatgtgt ttatgaaggg ctgctcttaa tgctgcatct
    11881 gttttatagt ctaaagcgga gaaactatca tcgaatacat agatctctgc tggtttgatc
    11941 aacgctcgag caatacatat ccgctgtttc tggccccctg aataattaga accgccttga
    12001 gcaacaaagc tgtccaatcc gtcaggaagg gtgtccacta gttcttctaa ttgagcgatc
    12061 ttgatcgctt tagacaactc ttctgttgag gcatattcat ttcccatctg caagttgctt
    12121 aaaattgtcc cgctgaataa gaaagccttc tgagggacat aactgatgtg ggagcggatc
    12181 tgttcttgag tcatttgagt aagaggaata ccaccaaggg ttacttttcc tatcgtaggg
    12241 tcactaagtc tcaagagaag tttcgccaaa ctactttttc cagatccggt cccgccaacg
    12301 atcgctgttg tttttccttt aggtattgtg aaatcaatat cttctagaac tggatcagag
    12361 atttcagtat atttaaatgt gacatgttca aaagcagctg catttgtctg atcaaaggtt
    12421 ggttcagcta ctgttttgat cgtctcttcc tcaatttcga tctctgcttg cagtatttct
    12481 tctatccgtc gtaatgaggc aaacgattta gggataatga ccaataccat agctgcgatc
    12541 atcaagtaag caagtgtcag catagagtat tcgactacag cagtcacgac accgatttgt
    12601 aatgctcctt ctgctacaag atttccgcca aaccacaaaa tagaagcata aacgattccc
    12661 atgagcaaaa aagcaattgg agtgacccaa gcaaatattc ggttcacttt gatcatttga
    12721 ttagcatagt cagcaaagct cttattcgtt cgcttttctt catatgattg attatcaaag
    12781 gcacggatca tattaatccc agtaaagaat tctctcaatg tgatggtgat ccgatccatt
    12841 tttggctgga tactcaaaga taaaggtgcc cctcgtttca tcagtcgata aacaatccat
    12901 acataaaaaa caacagagat caatgtaacc catgttagta atagagaata acgaaacatc
    12961 atgagaatgg tgaaaacgca aataaccggt gctggtaaga tcatttggca aaataaaaca
    13021 atcatttgct ggacattatc gacatcattc gtcattcttg tcaaaagaga tccagaacta
    13081 aaacgactga tatccttcat tgacagctgt tggaatttac ggaaatattt ctcccgcgtt
    13141 tggaatccaa acctagcgga agccaatgct gaaaagtaac tagaggcaac tgcagcgatt
    13201 gttcctagaa acgctacaag cagcatttca actccaattt tttttataac tgaggcttcg
    13261 ccacttgcta tacctgtgtc gatcagcttt gctactaatt ttggtacacc taatgtaccg
    13321 gcgatttcta aacagatcat cacaaaggta aggagagcga gtcctttgtt tttctgtaag
    13381 aatgttttta agatagacat attattcctc cttttttcct gatacttttc gattctatac
    13441 cttcaagtaa cttgaaggtc aatagaaaaa agaataaact gaaaaagagg tgagaaaaaa
    13501 tgaaaaaaat gtatacgatt ggccaagtat cggaactatt tgatttgcca aaatcaacga
    13561 tccgctattg ggatgaacaa ggattaatcc gttcaagtag acaagaagag aatgattatc
    13621 gattgtttga tatcgatgat atttttatgc tttatgatat cgttttttat agaaaattag
    13681 atataccgat caaacagatg aagaatttat atggaaaaac attgacggag ttatatgaga
    13741 cacttgatga aacagaaaga agaatccata aagaactggt ggtgatgaaa caaaaacaaa
    13801 aagagataag ggagcaaaaa aaacagttga aattgatgat cgatacaaac gaggaagaat
    13861 ttcctgtaga agagatccct tttgactgta tgatctcaac ggagtttgaa gatattgtgg
    13921 aaatcaaaaa atttctacct aactattcaa gctttgggat gatgagtatg ccagcttcca
    13981 gttcacagac gatgtatgga ttttttatcg accctagtga agtacattta tttactcaag
    14041 acgtgatttg ggagaaaaaa gatacggctg tttatcgtcg attcttattg aaatccgaga
    14101 tgaaccatgc ggaacgtaat aatattttag aaatcagaga aagaatgtat gaaaaaggat
    14161 ggaaaacagg agaagtgatc ggccagtatc tattgacgaa tacggatgaa aacaatatca
    14221 gaactgaata ttatcatgca tggatagaaa tgaaaaaata ggaaatatgt gtttaagata
    14281 actcgaaaat gatggttgtt ttagttataa agaaaaattt tctggttcga ttacgggttt
    14341 aatgtggggg attattagtc gtggatagat agaattttag ttttttagag gaaatatgtc
    14401 tttaaaaatg gaatggttgc aattcttagc gatatatacg atgtaattgt cctcttaaaa
    14461 aagtatggaa aaataattgt attgggtgtt atgtataatt catggtgaac tgagactaat
    14521 gagaagaaaa attcatattg ttatgagtga gattgtatca gatgagaatg ataaattaaa
    14581 taggtgcata aaagcattac acttaaaata cattgttgct tacattatta caatatgtta
    14641 ataaggcaaa aataaataga tggattgaat aaacatattt tttaatcaaa tagtccctac
    14701 tgtgactttc ctcagct
//
LOCUS       DARBFO010000064        14561 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000064 DARBFO010000000
VERSION     DARBFO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.065"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..777
                     /locus_tag="SCK51_002037"
     CDS             <1..777
                     /locus_tag="SCK51_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1252655.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDE
                     FFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            926..1264
                     /locus_tag="SCK51_002038"
     CDS             926..1264
                     /locus_tag="SCK51_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252656.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(1469..2044)
                     /locus_tag="SCK51_002039"
     CDS             complement(1469..2044)
                     /locus_tag="SCK51_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEG1252657.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2776..3357)
                     /locus_tag="SCK51_002040"
     CDS             complement(2776..3357)
                     /locus_tag="SCK51_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1252658.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(3540..4166)
                     /locus_tag="SCK51_002041"
     CDS             complement(3540..4166)
                     /locus_tag="SCK51_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1252659.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4188..5519)
                     /locus_tag="SCK51_002042"
     CDS             complement(4188..5519)
                     /locus_tag="SCK51_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEG1252660.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5674..6075)
                     /locus_tag="SCK51_002043"
     CDS             complement(5674..6075)
                     /locus_tag="SCK51_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG1252661.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6161..6367)
                     /locus_tag="SCK51_002044"
     CDS             complement(6161..6367)
                     /locus_tag="SCK51_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252662.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(6462..7964)
                     /locus_tag="SCK51_002045"
     CDS             complement(6462..7964)
                     /locus_tag="SCK51_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEG1252663.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7982..8437)
                     /gene="argR"
                     /locus_tag="SCK51_002046"
     CDS             complement(7982..8437)
                     /gene="argR"
                     /locus_tag="SCK51_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEG1252664.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(8535..9152)
                     /locus_tag="SCK51_002047"
     CDS             complement(8535..9152)
                     /locus_tag="SCK51_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEG1252665.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            9373..10746
                     /locus_tag="SCK51_002048"
     CDS             9373..10746
                     /locus_tag="SCK51_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1252666.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10821..11933
                     /locus_tag="SCK51_002049"
     CDS             10821..11933
                     /locus_tag="SCK51_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEG1252667.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(12005..13642)
                     /locus_tag="SCK51_002050"
     CDS             complement(12005..13642)
                     /locus_tag="SCK51_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEG1252668.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13810..14418)
                     /locus_tag="SCK51_002051"
     CDS             complement(13810..14418)
                     /locus_tag="SCK51_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1252669.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3992 a         3022 c         2614 g         4933 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtaga tgaatttttt
      661 attcaatctg tcgcatctaa acgaaataat attcctagaa aatcactgga ttatcgaaca
      721 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt aatttgacaa
      781 ttaagaaact aaaaaaataa tccaagaaac attgaaccta tggatccaaa agctacccaa
      841 atactatagc taaccccccg ctaccttcaa ttttgcagat aaaacgcttt gggagaaaat
      901 ttcaaagaca acaggccatc ctatcatgcc cgattataaa caaaaagctg gctattcctt
      961 ttgctctgac aaatttcaat tgggaaagtt cacgaaacta gcaggtgaag aagtttcaac
     1021 tgttcggatc aaagaatgcc cgcttcaaat agaaacattg gttcaacgaa tttcaactag
     1081 acaagacttt gctattgtag aacgtgagat cgtagctatt tttgtagaag aagggatttt
     1141 acatgatcca actcatatca atgtggataa atggcaacct cttatctata aattcagaga
     1201 atatacaact gccaaccagt cgttaacttt gaattttaga ttccaagaat tcaaagagtc
     1261 ataatgataa acatcgttag gtataaataa aaaagactcg cagcacgagt aaaaaacaaa
     1321 tagaagctat acattagaaa ggtcgactgt gaccctatag atgcagtata tgcagcttat
     1381 gagcatttct tctgttctct ttgtttttgg gatctgtgct gaagtctttt tcgttttttg
     1441 ccgctctttc tttgaaaaat cgattatgtt ataccttctc cattactaat tcaggcatct
     1501 gctgagtaat gatttttcgc gcaaaagaaa tatctgttac ccaaggttcg atttgttctt
     1561 cagtcaagac aagcggcatt cggtcatgga ttggtgcgac tgtgcggtta ggttcagtag
     1621 tcattatgat tgattctgcc attttttcat cctgatggct acgataaaaa ccgccaatat
     1681 acactatttt ttccttattt gaaaacataa atttgtcttt ttcttcattc cactcataaa
     1741 agccactcat tgggaaaaca caacgatttt gcaaaaatgg ttgatgaaag gtcttttttt
     1801 cttctactgt ctctgcacgg gcattaatca tcaattgtcc ttttttaaat ccggtaaatc
     1861 cccacttcat gatttccgga gtgaggatgt tttcttcctc ttttcttaac gtcacaacaa
     1921 gatttgacgg aaaaacttct ccagctgcta attctgctgc ctgcttcggt tgtttcgtct
     1981 gggcttctcg ataataattc tgaaggagct ctgactctag atcaaaaaaa taacgaccac
     2041 acatcctaat ccctcccaat ctttttttct atcatacact gcaacaagaa gttcgcacca
     2101 acaaaacgct ttcaattttt gtagaactaa aaaagttgtg acaagtgaca aactctccag
     2161 tgaatggtat tctcctgcca actcctcggt cacaagtcac aatattcaac aaacatgaga
     2221 agctgctttt tgaaattgtt ccttgtgact cggagtgaaa cggcaggctt acgacctctg
     2281 ggtaaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtag
     2341 aaagccattt tcacaaaatt tttcttaata ctcgaaacta agcgactttt tcacaacctc
     2401 ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat
     2461 atgagcagct atttttattt ggctgttctt attacttgat gcagggcagc tttttcacaa
     2521 cctctccact aaacggtgtt caatcagcta accttcggta ttagctcaaa aaacggcaaa
     2581 atatgaggaa ctattttgcc gtttgtttct ctaatactca ggtcaaagcg ctgatttcac
     2641 aacctctaat acacggtgtt ccagaagtag cttcttcgaa aaataagccg aaatttcaca
     2701 aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttctgt
     2761 cacaacctct aattattaaa gaaccttttg cgaagttaac aatctttctt tgatcaattg
     2821 aaaattctgc ctaagtagtg atagatcctg tgtgactttc aacaataaga tacttccctg
     2881 gatttcagca atcaacgctt tggcttgacg gtctgcttct tcatcggaca gtttttcttc
     2941 agaaagtgcc aggatatttt gtttcgtata cttgacccat tgattgacta attctagtag
     3001 caacagacgg tattcttcat ccactgtact caactcaata atcaaattac ctacaggaca
     3061 gccatgataa gtgatctgtg tctcatgatc agcgaaaatc cagtccaaca tttcttctaa
     3121 tgcttcttct ggctgttttc cagcatttaa gatgtcttcg aacaattctg tctgccatac
     3181 gtccatatgc gcatggatca ccgctagaca aacttctttt tttgaagaaa agtaataata
     3241 aaattgacct tttcctgtat cagaagctgc tagaatatct cggattgacg tttcatgata
     3301 gcctttttta taaataagat ctttggtcgc ttccaagatc ttttcctttg ttttcaatga
     3361 tattctcctt tttcatttct catagtccta tattatacag taaattcatc attttttctt
     3421 cttaatcgtt taaaaaagac tgtgccaaat tatctgacac agccctcact ataaaactgt
     3481 tctcatatag ctagatgttc attgattcaa gaactgcgag gacaaaagca attcctatct
     3541 tatgatagtt gctctaatac ttcctctgtt gtcatcactt gatctgcaat aaagccaaag
     3601 ttcgtcaacg ctgcatccat tgcttcttgg ccaggagcac caattgcatc tgtcgctacc
     3661 acaacattga atccactctc taccaattca cgcatatggg aatcgctaca caagtttccg
     3721 ttcattccac caagaattac tgtgtcgatt ccattttttc tcaattggag cgccaaatca
     3781 ttgctttctg gtccaaagat tttatgagga gagctgatga tcgtattttc atccaaataa
     3841 ggtttcaatt cttcgataaa atcggctcct gaaccttctt ccactgattc atattgactt
     3901 tttctagcaa acatctgctg ctcaagcatc atatgttctc ctgcaccgcc aaattgccat
     3961 ttatggtcat gtgggtaaaa ataatgagga gaaataaaga gtttgtatcc tttttctttt
     4021 gcttgtttta ccatttcaat catattttcg atcgtattgt atttttctaa gatatctttt
     4081 gtcaaaccaa agccaccgcc tgctggtttc aagaactcga cttgcgggtc agtgagaact
     4141 actgctgtat ttttattatt gaacatggtg gattctccat tcgttattta ttttaataca
     4201 tcatttagag cttcactcat tgtcggatga gaatagattt gatctctcaa taacgtataa
     4261 ggaagttttc cattcatagc tagcgaaatc aagttgatga cttcatgaga atcttccgca
     4321 tagatacttg cccctagaat caagtcagtt tcagggtcga ctaaaacttt gaatacacct
     4381 ttaggatctt ctaatacttg tgctttaggg acaccgcttg tcatatactt gaataatttg
     4441 taattgacgc cttgtgcttt ggcttgtttt tctgttaaac ctacattgga taaagtcgga
     4501 gtgataaata cactgtaagg cacagctttc cggttattcg ttgttcgttt attctcgccc
     4561 ttcaactgat ccataatgat tcgataatca tctagagaga tataagtgaa ctgcaatccg
     4621 cctttgacgt ctccaatcgc ccaaatgttg tctgcatctg tacgaaggta ttcatccaca
     4681 gcgattgctc cgcgatctgt tagtttaaca tctgtatttt ctaatccaag tgatgctgta
     4741 cttggttttc ttccagttgc agccaaaact tttgacgctt ggattgttac ttcttggcca
     4801 tcttctgtaa aggtgacggc agctgttgat tcttggtctg ctacttgatc tacagataca
     4861 cctagatgga attcgattcc cgcatttgtc atgtcttcta aaatcatttg cgcgatatct
     4921 tcatcttcac gtggaagaaa ttcagaatgg gcatcaagaa cgacaacttt agatccgtat
     4981 tcattgaaca ttgatgcaaa ttccaagcca atatatccag ccccgatgat cactaatttt
     5041 tccggtaatt gttcctgatc catcgcttgc gtagaatcaa tcaagtagcg acttgtattc
     5101 aatcctttga ttggtaaaat gattggttca gaaccagtat tgatgaaaat ccgttcgcct
     5161 tcaacaatca ctttttcacc attattcgtc acttcgattt gatggttaga aagaaaacgt
     5221 gctgtaccgt ccatcactgt cccagttgcc tcatcgctga tcatatgata atttttggcg
     5281 cgaagcatgc ccgtcaattt ttctttccga gcaacagcct ctgtaaagtc gattcctctt
     5341 tctccattga aaatcaatga tttagaagga atacagccga tgttgataca tgttccgcca
     5401 tacatacgtg ttgacttttc aacgactaaa accgattcac cttttgaagc taacgccttc
     5461 gctaaagtct taccgccctt tccaaaaccg atcacaatat tttgatattt ttccatcatt
     5521 tttccctcgc ttctttttta cctaccgatt ggtacaaaaa tatgataacc gataattgtc
     5581 tattctccaa atcatctgct ttatttgtca aaaaaaacca gacaaaaagc ggtcctttca
     5641 cgtttttagt ctggttcgat ttcctttatt cttctaaaca atcgttactt ctacatcgat
     5701 atttcctcta gttgctttgg agtaagggca gaattcatgc gcatcttctg caattttttg
     5761 ggcttcttct tcgctgatgc catcgatatg cacttctaat actacaccga tctgatagcc
     5821 gccatcatct aaattataaa gagagacacg tgcttttact gtggactctg ctgtcattcc
     5881 tgcttctttg atcatatgtt gtaccgcccc attgaaacaa gaagcataac ctgctgcaaa
     5941 taattgttct ggatttgttc cttctgcatg gattcctgga ggagtgattt tcatatgcat
     6001 cgagccattt ggtgcctcga cttctccatt gcgcccgcca tggttgatca ttgctgtttc
     6061 gtatgctttt ttcattgtac tgctcctttc gattgaccat ttgctacttg gctataatta
     6121 tagtgtatga caagtaaggc gctttctaca aaaattctgc ttattcgaat accagaatgc
     6181 cgtcttctac tatttttcca aaaaacaaaa aaatactact cgtgaacaac agcgtcatat
     6241 ttgttccaaa ataaatagca atcttttcta cataatcttt tatatgattg attttaagat
     6301 acatatatac cagaaagttg accccataaa ccaatactgt taaaaaaata accatattcg
     6361 ttaccatttt ttcctccttt tgctggtctc cctaccagca ctcgatttaa ccataccact
     6421 ttttttgccg tccaagcaac tagcctttca aaaagactgg tttattctgc tgttaatgat
     6481 tcaagatcag aaaaatgtcc gaataatttt gctaatttta ctaacgccaa cttgttcttt
     6541 ccgaattcat cattttctat ctgtaatact agtaacggct catgtaagct taaacggatc
     6601 aaaaaccagc cgtttccgta tggctcttct agatcatatc gaataccttc ttcattttcc
     6661 gggtcgaagt tcatcccttt ttcttcttcc aacagtcgct tcatttcttc taatatcttg
     6721 tttccttgtt cgcgtggttc atcgctgaac aattttaatc ggatttcttg tacttctacg
     6781 ggctgtttca actctttgat ttgttcatct aatgtgatac cttttctttt taattctggc
     6841 aaaagcatca ggatctttgc gatgacatat gcaccatcat ccagaaaata gttttccttg
     6901 aatgcagcgt gtccgcttgt ttcgatggct agctgtgtat ctttcccaag acggttaagt
     6961 tctaatgctt tattgattac atttcgatag ccggaaatat aacgaacttg gatgcctcct
     7021 ttttcttcaa tgaatctttg tagatgagaa gaagtagggg aattcgtcac gattcttgtt
     7081 cctggatgct cttttaatac gattgtactt aatactgcga tcaaattatt tcgattgatc
     7141 actgttccag aagcactgac aactgcagaa cgatccacat ctgtatcgaa aatgatccca
     7201 agatctgctt catttgctaa aacagcctgc ttgatacttt ccatcgcttc tttattatca
     7261 ggatttggga tatgattagg aaatcggcca tctggttcca aaaattggga acctgttgaa
     7321 tccgcaccta agctattcaa tactttttca gcaaagaatc caccagcacc atttccggca
     7381 tcaacaatga ttttccaacc atttagtggt ttgctttcta gtgttccaat ccccttttta
     7441 atttttgtga ctaaatcttg cgcataaggt gtcaaaaggt ctgctttttc tacgcttcca
     7501 gtttgttcgc tttttatttt ttgctgctgt gataggataa aagcaatatc ttctttttct
     7561 gctcctcctt ctgaggtaaa gaattttatt ccattataat aatagggaag atggctagca
     7621 gtcagcataa ccgcagcatc gcacgagaac tgttcaaatt gagtggacat aaacatagca
     7681 ggcgtagtag ctaattcgaa atcgatgaca tgtatccctt gttctttcag actttggatc
     7741 aacgcctctt tcagtgctgg tccgcttatc ctactgtcat gaccaatccc aatcgtccat
     7801 tttttatttg gatcggttga tttcttcttt ttctttatcc agtttgctag tccccagccg
     7861 atctttttta ccatgtctgt cgtcaagttt acttcatatc cttcaactgg taatgcgatt
     7921 cctcgaatat ccgatccatt ttgcagcgct ttcatatctt tcatataaaa gtcctcctca
     7981 tttattccct cattttttcc agcttttctg caaatcgctg tgcttcttct tctgagcggc
     8041 aaataataat gaatgtatct acaccggcca tcgttccgac aacttcaggg taagccactt
     8101 catctagcca attcgttacc acatcggcac catttcgttc agtcaatacg ataaccatga
     8161 actgaacaga ctggatacgc aaaacctcgc gtctaattgc tgtacgcaaa cgttcttcat
     8221 ttaaagaaac agatggctgg ttaaataatg tatagcgtac tattttgttt tcattttgac
     8281 tttttactaa cttcatttct cgaatgtctc gagaaatcgt tgcttgagtc acatcggcgc
     8341 ctgcttcatg cagccgcgca agcagctctt cctgtgtccc aataggttgt tctgtaatga
     8401 tctggcggat cagtgcttgt cgctgtttct ttttcatatt agacacctct caatgttgat
     8461 tatagcaaaa gataagtaat atttcttcat attttggtat tttttacttt tattttttgt
     8521 tttcattcag gttgctaacc tttttctatt ttaacacgca aaatcgtctt cattttttca
     8581 ggtgctgatt ttcttggatc gctgatgtaa atctctttgt gttctttaga agctctttca
     8641 tagccttctt ttgcgatgaa tgcttctaat ttctcaaacg ttgctggttc ttcggcatag
     8701 ggcccgacgt gtaagatttg ggcacaaaga ccttcttcca tctttttgaa tactagttgt
     8761 tctttcaagt cttccggaag tttgctagaa cgctgaatgg cttcttgtgc tatcgcttct
     8821 gtaacaaagt caggctgtcg gatcatcaat tgataagaaa aatgttcttt tttcatcact
     8881 ggttcatgca aatacttttc ttgcagtccc cattgtcctt ccagtggata gacagtatac
     8941 atctgataat caggaatcag ccaatttttt ttaggcgaca tgcgtattgc atagctgact
     9001 gcatatagac aggctactct tcgtttaaag tcttcctgat tcgggtcgcc gatccctctg
     9061 atgctaaaga atggtttttt aggaacctct agtatttgcg ggcttttttt catactatag
     9121 ctcttgtctt tacgccattc aattttttcc atttttcttc ctccgcgaaa ttccatttag
     9181 ttatttatta tcatacctct ttttgagtga cttggctaat cgtgttgaca ctatttttca
     9241 gtttgtgcta gaataagtga agtaaataaa tcgtacagat aacacgtttc ggcgtgtcga
     9301 gagagaggcg gtttctattt ttataataga aatcactctc tttttttacg aaatttttta
     9361 gaggtgatgt aaatgacaaa acaaaaacag ctgcttttag tacctgcagt aattgcaaca
     9421 gggatgatgt cttttgcggg cgtattgatc gagacggcca tgaatgtaac tttcccaact
     9481 cttatcagac agtttgattt aacgactgca caagtccaat gggtaacaac gatttatctt
     9541 ttgatgattt cgatcattgt tccgctgtct acttatttga atcgcaattt tagtctcaaa
     9601 aaactctttt tagcttcaaa tctgcttttc ttggcaggta taatgatcga tttcttctct
     9661 cctaccttca tggtcctatt gtttggtcga ttgctccaag gtgcagcgac aggtatcgct
     9721 cttccgttga tgttccatat tatcttgaca tttgcccccc tcaatcgacg aggcgcgatg
     9781 atgggtgtcg gtactttgac aacggccatt gctccagcaa ttggccccac ttatggaggc
     9841 attatgactt cacattttac atggaatcat atcttcctgt ttcttgtgcc cgttttgatt
     9901 ctttctttga ttatcggctg gtttgctatt cccgagatgg cagtacaaaa aagtggttat
     9961 ttggattgga taagcgtttt aggaatttgt cttttgttta gtggaagtct aacatttttg
    10021 agtacgttgg gaacagtttc cagctggatt gctttaatca tcggcggtat tggctggttc
    10081 cttttttact atcgaagtaa aaaagtggag catccactga tccgactgtc tattctttca
    10141 aataaaacat tccgcttgtt tttgattggc ttcttagttt atcagttttt attacttggt
    10201 gtatcttttg tcttgccgaa tttccttcag atcgttcaag gaagtagtcc ttttgtcgct
    10261 ggattagcga tgcttcccgg cgcagcggtt ggtgctttgc tttctccttt ttccgggaaa
    10321 atgctggatc aatatggtcc gaagaagcca atttttgctg ggctgattct ttctttgatt
    10381 ggttggctag cattattcct actcatagga cacgcttcag tgggtgtatt gattgcttgt
    10441 catgtgtttt atatgatcgg tatcggatta tcttacagca atatgatgac caccggaatg
    10501 aatgcactga atgaagaact acaaggagac gggaacgcaa tgttcaatac cttgcagcag
    10561 ttttctggcg ctgtggctac ttcattagta gcggtcatta tcaattacgt ccagcatcat
    10621 acttcccata actatgaagt gtctactaca ttaggatcaa aagcagcatt aggcgtcttg
    10681 ttgctgttgc ttctcgtaag ctttgctcga ttcgcctatt atctgttctt tgctaaaaaa
    10741 gcgtagattc catatacggc aggtgttgca tctgccgttt ttttgtgtga aaatggttgt
    10801 ttgtaaaagg aggctttttt atggaaaaaa ttgccattat cggtggcggg atcattggta
    10861 tgactctagc cagttactta gatacatcaa aattcaatgt tgctttattt gatgaaggga
    10921 caggacaggc aactaaagct agcgctggga ttatctcccc ttggttgtcg aaacgcagaa
    10981 acaaacaatg gtatcgtctg gctaaagacg gcgctgcttt tttccctaaa cttataaaag
    11041 atttccaact tgatcaaacg atttacgcac aatcagggac acttttgctt cgacctgaaa
    11101 aagatttagc tgaattggct gttcttgccg agaaacgaaa agaagaagcg ccagaaattg
    11161 gaaacatcga atggctttca cctgaacaga catctcaagc acttccgtta ttgaaaccag
    11221 cagtttcact gcgtatcagt ggtggtggtc gtctggatgg aaaagcatat ttagaacatc
    11281 ttggtcagcg tttagcacaa aaacaaatgg agcagatcca ggcaaaagtc actttgcata
    11341 agaagagcga ttcttggctc atcaagggac cgtttggaga atacacagct gatcgtgtaa
    11401 ttttgacacc tggcccggca cttaagcctt tattacaaga attaggctat gctgcagata
    11461 tacgtccgca aaaagggcag ctcttagcat ttcagacatc ttataagatg agtcaagact
    11521 ggcctgttgc tatgcttgac ggagaagctg atttcattcc tttcatggat ggaaagatcc
    11581 ttttaggtgc tacccatgaa aacgatcaag gctgggattt ggctcctact agcgaagctt
    11641 ataagggatt gactaagagt acagaacagt tcttgactga tccagatcag ataaaaaact
    11701 ggccatacga gttccgtgta ggtacccgtg cttatacgtc agatttttcc ccatttttcg
    11761 gcgtactgcc agatgatcca agcttactgg ctgctagcgg tctaggctct tctggcttga
    11821 ccacaggtcc ttatatcggc tatctattag ccaacttctt aaatactggt cattggtcta
    11881 cggaagatta tcaaaaggac atctccacct atattacgaa aaatccatca tagaaaaaca
    11941 gctttttcaa gtaaggatcc cgagcgatat tgcgcatgga ttacttcttg aaaaagctgt
    12001 tattttagtt caatttctga atcaaattct ccaaactttc ttttgcttca tctggcaaga
    12061 gatcttttcc tatctgagct tcaatgaatc gtaaacgaat atcaagtcgt tgtttcacca
    12121 gatttttata ggacgtatcc tcaaaagaaa caggaaaatc tggtataggt aaatagtgga
    12181 tgtcttcaaa tggaccaaac gacttgaatt ctgccgtttg ttgcacgatt ctttcaacga
    12241 ttgacaatgt cgtttcaggt ttgatttttt tatcatcgaa atatccagta ttcaggacat
    12301 agcaggctgt ttgctgtttc aaaaacaact ctcgaaaagc ttgatagtct tccctaagag
    12361 gatagacacg gaaaggatta gcaaatggtt caatgactag ctcatctagc tgtctttttg
    12421 tagtgatatt ttccgcgtct gatcgttttg ttgctaatgt tgcaccaaaa gacgctgcta
    12481 aaactgggtc attgatcttg ataattggcg gaagcgaatc atctttcatg atccaaaaaa
    12541 cagcatcgat cttttcgcta agatgatcga ctcggtcggg tgtcgcataa cgtgatttga
    12601 ctgtccgccc atttccgttt cgtatatctc ctgttactag gactttttct cctttacttg
    12661 tacatgtcac gcccacattc tgacaagtaa ggaaagaaga aatggccgga tcatcagacg
    12721 gatagtcttg tgttttatca aagtaagaag gttccaaggc agtagcaaaa ccattttttc
    12781 gagaaataac gaaagcatcg tcatgcaaga tcgaaacaga gtgaccttcc gtatgttttg
    12841 ctaaggtaat cgttgatttt ccagaaccgg acaacccaaa tacagccatc gtataggtct
    12901 ggtcattact agcatattgt ttcatcccac cgtggcaggc aataaagcca ttgcgatttc
    12961 ccaatgccca agcaagcgtc aaagtcgctt tttttaattc tccaaagtaa cgtaatccta
    13021 gcacagccgc tgtattatgg agagcatcaa acaaaatgag tccatttgga aatgtttcat
    13081 gtgtccattc gggatcacaa aagatataaa gatctccctc atcaaaggaa acagactttt
    13141 gataagtctc taagatcttc gaattcgctg gctgaaaatt cagcaaataa gaatacaaat
    13201 tgttctcaaa tccttctgga atcagcaaat gactttgcac agaaaattct tcagataggc
    13261 caacgattac ttttgtatgg taaaactggc gcttgcgact ttgatacaca gcttctctta
    13321 aaattttctc gtatttgctt tgttccttgt ctaagtcctc aaagatccgc cgagcacttg
    13381 ctgttcttcc cacaatgctt ccatcattga atactagtgt cctaacatca gctggtaaat
    13441 ctagttcttc agcatgtgag attgttaaat ctgtgatgat cgttcctgga ctcccttttg
    13501 ctagttgata agctgtcttc ctgtcaaaaa cttcatgaac attattaccg taaaatgtcg
    13561 tttctacaat cgatcgaata ctggataaca gtggatttcc acttgtgatt tcgtttaatt
    13621 cgagactctc ttttgtactc atggctcctc ctcattccct aaacaaaaaa acgttttctt
    13681 tattatacat tattcgagtt tttatttgta ctgctgcgct gtgggaagca aacgagttat
    13741 ttttcacaca aaaaaaccga gaacttgaat aaacaagttt ctcggtttca tcattttcct
    13801 tttatcggct tagtaccagc cgtttgctaa ccagaatgtt ttagcagcat cccatgaacc
    13861 ataacgagat gttacatatt gttctgctac acgttcttga ttggcagctg aatagtcgcc
    13921 gtttaagtat gaagcatcta attgataacg gccgatgtaa cgtccgttag tagctgtgta
    13981 tgaaccgcct gattcttttt gtgcgatcca ttcttttgca gagcttgaag ttggcgctgt
    14041 tgcagcagct ggggcagcag gagctacttc aactgtttca acaggtgcaa cgtatgtatg
    14101 ttcttgcgtt gctgtttcag ttgtagatgc tgtattttct gttgttttgt tttctgttgt
    14161 tgctgaagaa tcatttgaag ttgggattgt taattgttca ccaacataaa tgcggttgat
    14221 gtttgcgatc gaattttttt cagcaattgc atcgatcatt gagttgtctc ctgcaaattt
    14281 ttgtgagatc ttagataatg aatcacctga ttttactgta taaacttcat cagcgtgagc
    14341 atttgtagtt cctacaaaca ttgcagtagc accagcagca aaagttgtac ctaaaacaat
    14401 tttcttaaat gaattcatat taaactcctt tttttctccg ttttttattc tttcgacgct
    14461 attaacttta tcatttagaa atatttcata ggtaaaagaa acatgacatt taatgacaaa
    14521 ataacgaatt atttaatatt tgtaacattt aaacaaatat t
//
LOCUS       DARBFO010000065        14483 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000065 DARBFO010000000
VERSION     DARBFO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.066"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(287..649)
                     /locus_tag="SCK51_002052"
     CDS             complement(287..649)
                     /locus_tag="SCK51_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252670.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="SCK51_002053"
     CDS             complement(723..1103)
                     /locus_tag="SCK51_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEG1252671.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1167..2360
                     /locus_tag="SCK51_002054"
     CDS             1167..2360
                     /locus_tag="SCK51_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1252672.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2437..3021)
                     /locus_tag="SCK51_002055"
     CDS             complement(2437..3021)
                     /locus_tag="SCK51_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG1252673.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="SCK51_002056"
     CDS             3222..4220
                     /locus_tag="SCK51_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1252674.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="SCK51_002057"
     CDS             4332..4841
                     /locus_tag="SCK51_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEG1252675.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="SCK51_002058"
     CDS             complement(4871..5530)
                     /locus_tag="SCK51_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEG1252676.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="SCK51_002059"
     CDS             complement(5532..6296)
                     /locus_tag="SCK51_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEG1252677.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="SCK51_002060"
     CDS             complement(6304..6981)
                     /locus_tag="SCK51_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEG1252678.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="SCK51_002061"
     CDS             complement(7018..8403)
                     /locus_tag="SCK51_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG1252679.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="SCK51_002062"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="SCK51_002062"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="SCK51_002063"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="SCK51_002063"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEG1252680.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="SCK51_002064"
     CDS             9342..10199
                     /locus_tag="SCK51_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1252681.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="SCK51_002065"
     CDS             complement(10280..10615)
                     /locus_tag="SCK51_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252682.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="SCK51_002066"
     CDS             complement(10682..12052)
                     /locus_tag="SCK51_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEG1252683.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="SCK51_002067"
     CDS             complement(12130..12666)
                     /locus_tag="SCK51_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1252684.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12975..14456
                     /locus_tag="SCK51_002068"
     CDS             12975..14456
                     /locus_tag="SCK51_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1252685.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4461 a         2884 c         2353 g         4785 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 gctatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc
    12781 cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca
    12841 aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa
    12901 cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata
    12961 aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg
    13021 tcattcaaaa aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac
    13081 ggacaatcaa agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg
    13141 atgaatttat ttattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta
    13201 aaaagttagc tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta
    13261 actatttgat gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg
    13321 aacaacttta tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct
    13381 taaaaaaatt ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg
    13441 agttatctat ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt
    13501 ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat gagcaaatag
    13561 aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt aactttattc atattacgaa
    13621 acaaacaaca ggcatatttt ccaaaaactt cttctgaaat cactgaattg atggacttat
    13681 tgcagcatac acatgattat tcagaatact ttacttcttt ttcagtgagt cgcccttgtg
    13741 aagagacaga aacagagaga gcctacttta attatttttt ctattgtttt tttccaaatc
    13801 aaatctcttt tcgacaaaaa caggagattg gttttttatt ttccacaagc aaaaacctta
    13861 tttgtcgaca agtcggtcaa ttgatgactt acttaactca acagtttcct ctcaaccatt
    13921 tagaaaataa aataccaatt tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac
    13981 tgttgggtga atccagcagt agtttttcta cactttgctt tccagaagcc acgtccaatc
    14041 attctagttc ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa
    14101 aaaatgggga aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac
    14161 aaacagaaac gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa
    14221 attatcatct tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg
    14281 catattcaga acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa
    14341 cggagacttt ctatctggat acaacgcaca atacagtgtt atttggcaag ttaataaaag
    14401 cggttcatga actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa
    14461 attcttgtca catcctctta ttc
//
LOCUS       DARBFO010000066        14342 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000066 DARBFO010000000
VERSION     DARBFO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.067"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            108..489
                     /locus_tag="SCK51_002069"
                     /pseudo
     CDS             108..489
                     /locus_tag="SCK51_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            620..1570
                     /locus_tag="SCK51_002070"
     CDS             620..1570
                     /locus_tag="SCK51_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1252686.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2088..2543
                     /locus_tag="SCK51_002071"
     CDS             2088..2543
                     /locus_tag="SCK51_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1252687.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2941..3486
                     /locus_tag="SCK51_002072"
     CDS             2941..3486
                     /locus_tag="SCK51_002072"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252688.1"
                     /translation="MELAEPGHYDEKWQNWKLESLPIFPDRYDFEVAKDKGKQFKIVA
                     ELLKKANTIIVATDSDREGENIAWSIIHNANAFSKDKTFKRLWINSLEKDVIRSGFQN
                     LQPGMNYYPFYQEAQTRQIADWLIGMNASPLYTLNLQQKGVQGTFSLGRVQTPTLYLI
                     FQRQEAIENFKKEPFFEVEAK"
     gene            3580..3912
                     /locus_tag="SCK51_002073"
     CDS             3580..3912
                     /locus_tag="SCK51_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HEG1252689.1"
                     /translation="MGNQEGIIADVQTKEKKTNSPSSFSLSSLQSKVNQLYKATASQT
                     LKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIRSN
                     NSTQILTN"
     gene            complement(4063..5042)
                     /locus_tag="SCK51_002074"
                     /pseudo
     CDS             complement(4063..5042)
                     /locus_tag="SCK51_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5047..5814)
                     /locus_tag="SCK51_002075"
     CDS             complement(5047..5814)
                     /locus_tag="SCK51_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1252690.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6304..6729
                     /gene="lacA"
                     /locus_tag="SCK51_002076"
     CDS             6304..6729
                     /gene="lacA"
                     /locus_tag="SCK51_002076"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEG1252691.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6746..7261
                     /gene="lacB"
                     /locus_tag="SCK51_002077"
     CDS             6746..7261
                     /gene="lacB"
                     /locus_tag="SCK51_002077"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEG1252692.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7272..8204
                     /gene="lacC"
                     /locus_tag="SCK51_002078"
     CDS             7272..8204
                     /gene="lacC"
                     /locus_tag="SCK51_002078"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEG1252693.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8207..9187
                     /gene="lacD"
                     /locus_tag="SCK51_002079"
     CDS             8207..9187
                     /gene="lacD"
                     /locus_tag="SCK51_002079"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG1252694.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9215..9532
                     /locus_tag="SCK51_002080"
     CDS             9215..9532
                     /locus_tag="SCK51_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1252695.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9532..11238
                     /locus_tag="SCK51_002081"
     CDS             9532..11238
                     /locus_tag="SCK51_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1252696.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11380..12786
                     /gene="lacG"
                     /locus_tag="SCK51_002082"
     CDS             11380..12786
                     /gene="lacG"
                     /locus_tag="SCK51_002082"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEG1252697.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12809..13081
                     /locus_tag="SCK51_002083"
     CDS             12809..13081
                     /locus_tag="SCK51_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEG1252698.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13102..14001
                     /locus_tag="SCK51_002084"
     CDS             13102..14001
                     /locus_tag="SCK51_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEG1252699.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4685 a         2363 c         2765 g         4529 t
ORIGIN      
        1 gacactatcg ttcttcatca acgtaagtta gatttgttga aagagcagaa aaaaggattt
       61 ctacaaaaaa tgtttgtttg atattttaaa tagaaaagag agataaagtg aagtttttaa
      121 attttttgaa ctataaatat ttaaactggg tattatatgg tattacatta ttttttatta
      181 tttattactt ttttaccgat gatgatattt ttttaatttt agcaatactt tttcttgttt
      241 catctaattt tagtgataac ttaattgaag ttcttacaga accagaaatt cggtctatga
      301 cggatgaaga acaacagatt attaaagcta tgatttcgga aaataagaaa gacgtagtag
      361 ttatcaaaaa gattagagag tatacaaatt taggtgtttt acaagccaaa aaagtatatg
      421 aaaaaaaaaa aaagggtcta taattacaaa ttagataata cattagaagc tggagacaag
      481 attgtctaga taatgtgtga gataaaattt ttggcaggtt atcatacata agacaaaatg
      541 tttttaaaac tgataacgta tttattccac aacaattacg catttatttc tgagaaaaat
      601 ataaagcagg aggattacaa tgctgaaaac ttattcctta acaaaagata cattattagt
      661 ttcgtcagaa tcaaatatca aaaattcttc tgttgtttat gtttatggtg acgaacgaga
      721 atatatcgaa tcttctgaac ataaatatga ctttaaaata ggcaatatac taacgtttga
      781 tgatcgagtt gcatatgaca cgatgataga taagaacgat gaaaaaagtt tgctcgtaag
      841 ttttttgttt cccgaggtac atgaaggtgg gcatctagat accctgacga catctgttgt
      901 tttcttggta tttaaaaata agttgataat ttttacaaaa caaaaattaa aattcattcc
      961 cgaactctta tccaatatgg ttttacgtga tgtcaatcat ttcgtggagg aagtactttt
     1021 aaagatcatg caaaaagatt tctatatgat gcttaacgac cttcgtggag tcaaggaaaa
     1081 aattgatgat ttagactcag aaatatcaac gtccggttca ctcaaaccaa cgtttggtga
     1141 tttattgact cttcaaaaat acatgatagc tttgtcaaca acttataaag ctaaccataa
     1201 agcgcttgat ttcattaaaa aaaatttcac aactattgac gatattgatt ttagtactaa
     1261 aaaaataatt gatgatttat acgatacaag tgatacaatg gatagaatca ttttaagtta
     1321 cagccaatat ttggacaaat tggaaaatat gataaacaat atgatttcgt atcagctaaa
     1381 catgattatg aaaaccctaa ccgagatatc aattgttttg actattccag caattatttt
     1441 tggcttttgg ggaatcaatg tccatgtccc gtttgaaaag tcttcttatg gtgttttagc
     1501 cgtattgatt atctctgcta tactaagtct aatttgctgg cttttgatgc gacgcaaaac
     1561 atatttgtaa agtatttgtg ataccctaat atgatttaaa gcagaatttc tttgttgact
     1621 ttttaaaagg ctgattcctt tcaataattt aatagcttca gcggctgctc agaagtcaat
     1681 aaattggact atttttattc agttacttag gcaacatttg agatacgatt gcgattataa
     1741 aaattgatat accaaccaat ggctgcttgg aagactgaca tattagcctc gatatcatcg
     1801 aataactaaa tacagaaaaa tacaataaat attatcaaac tagggactat actagaaaac
     1861 atcagtacat tccttaactc agggtctata attagaaaat cgcccctttg aaaactgatt
     1921 aaaacagccc ccattatcta gtgactagat aatgggggct gtttttttgt taagtgatat
     1981 aataaaccat aaaagaagtt tagaaatacg gtcctggtca tcaataaata aacaatttaa
     2041 ttaattattt atttaattat ttaatttcag aaaggtatac atgaatcatg gtgcaacaaa
     2101 ttgtcctacc catcaaagac tccagcgtct taaagatggt acaagataca cttctcgata
     2161 gtttccgtgc tggtcgccga aattacaccg tttttcaagt tggtaaggcc acgctactac
     2221 gtgtgagtga tgtcatgaca ttaaaaaaat cagatgtcta taatccagac ggctctgtca
     2281 aaaatgcaac ctttattcat gataaaaaga ccggtaaagc aaacacgtta tatttaaagc
     2341 ctgttcagca agacttgttg caatatcatg attggctggc ccaagaaaat atcaattctg
     2401 agtggttatt tccctcgaca gcccattatg atcttcatat cactgagaag cagtttcata
     2461 aggtgatggc gcatgttggt gatctcttag gaatcaactt ggaagacaaa gagaaaaaat
     2521 tgcgagtcag aaaaccgttt tgagatatga acagcatttg ccttcacgcc ttagcgattt
     2581 taaataatag ccttgaaagt gattatggaa aagccctcta cgctcaatgc aaaattgacg
     2641 tagaaactat ttttgatcga ttgaaaggtg tatttggtat gtgaagaacc catgtcagag
     2701 gcaagaacgt tgcacctact gacattggaa tgatgcttat gagcatgaat ctaaccaaat
     2761 tatcccttga ggctagaaga aaagcggaag cttttcaaca caaagcagcc aaaaataaaa
     2821 aacgcgacga agcaatcaca tttatgatta ttcatcgcgt tttttatatt tagagctagt
     2881 tatttgctag acccactatt tacagatgaa acgtttatca cctttggttt tgggcattta
     2941 gtggaattag cagaaccagg tcattatgac gaaaagtggc aaaattggaa acttgaatct
     3001 ttgccgattt ttcctgatcg atacgatttt gaagttgcaa aagataaggg aaagcagttt
     3061 aaaattgttg cagaacttct caaaaaggca aatacaatta ttgttgcaac agatagcgac
     3121 agagaaggtg aaaatatcgc ctggtcgatt atccataatg caaatgcctt ttcaaaagat
     3181 aaaacattta aaagactatg gatcaatagc ttagaaaaag atgtgatccg aagcggtttt
     3241 caaaatttgc aaccaggaat gaattactat cccttttatc aagaagcgca aacacgccaa
     3301 attgccgatt ggttgatcgg catgaacgca agccctttgt atacgttaaa tttacaacag
     3361 aagggcgtac aaggtacatt ttcactagga cgtgttcaaa cgcccacctt ataccttatt
     3421 tttcagcgcc aggaagccat agagaatttt aaaaaagaac cttttttcga ggtggaagct
     3481 aagtaaaaca agggtcattt aagggcgttc taagccccac acagcgtttt aaaacccaag
     3541 aggagctttt tagcttttgt ttcttctaaa caagctaaaa taggcaatca agaggggata
     3601 attgctgatg ttcaaaccaa agagaagaaa acgaatagtc cgagttcgtt ttctttaagt
     3661 agtttgcaat caaaagtcaa tcagctttat aaagcgacag cgagccaaac tttaaaagct
     3721 atgcaaggac tgtatgaagc aaaattattg agttatccaa gaacagatac accatttatt
     3781 acagagaacg aatttgctta tttaaaagcg aattttggca aatatagcgg ttttttagga
     3841 cttgatcttg aaatgttgtt atcattgata atgatacgtt caaacaacag cacccaaatt
     3901 ttgacgaatt agtgaaactc tatgaaaaag acgtagtaaa acatgcaaca tcctattcta
     3961 atcagctggt aaagcttaat taggttatat aagcttgaaa ataaagggtt tgtccaatat
     4021 ggacaagcct ttttcttatg ttttgccaca gatttgccac acttattgtg taagagtatc
     4081 aaggataggt ataatttgat actttgtttt tttagtgatg tgcaaataaa tttctgttac
     4141 acgactacct ctagaatgcc ctacacggtc ttgaattgct tctaggggta ttccttactc
     4201 tgctaagaaa ctaatatgcg tatgacggaa tatatgggtc gtaatatgtt tattaaaact
     4261 aagtttatta tcaatatcaa ttcctgctcg ttttggatta ccttttcgat ttaattttcc
     4321 attttcaggt gagttgagga aattgttaag gacactataa tgaagaggtg ttccattata
     4381 gtcgggttta tgacgacttt tggaagtaaa aatccatcca ttatctttat aatcaggatt
     4441 gaatctcata tgttggtgat ttaactcaat ttcctcttta agaatttcta tagccctttt
     4501 agataattgg acatttcggt aactttgtaa gttttttaca ttaccttcat agctattttc
     4561 tgcagctcct ccgtgagtgg actgactaat gatgatattt tgcttttcaa aatattgata
     4621 ggcaccaata ttttggagtt tcgactctgt aagtcaacag ggccattagt tagaatacct
     4681 attgggatgc ggtttttatg tagagccagc agtagttgat gtatctcagg aatcatcgta
     4741 atctgatcga gttcatattc atagtcagct tggaagtcta gagcttctgt cctagaaata
     4801 ctatcgatgc caaattcttc catagtcttc tgacagcgaa aaattctaag ctgctcaata
     4861 ctgatgagtt ttttcgtgta tttaggaaaa gcaacatcag accaatatct gaaacgtttg
     4921 taaatgttat ctatttgttg gatatcaatc gttggaaaac atttctcaac gctacgttta
     4981 aaggggagtt gatggtcata aagagtgtca tcaaggtcaa aaacaatatt tttataagtc
     5041 ataattctag tccgctttta aaatagtagt gtactggcta aattcctcta agtgttgagg
     5101 gctaatctgg ttatctgtaa tgatggtatc gagttgatct agattataga agttaaagaa
     5161 atcgtatcga tcgaatttag tactgtctac taataagaat ttttctacag cattgtttag
     5221 ggcaagttgt tgaatcacac cttccttgtc actatatgta gcaatagaat tatgggtaac
     5281 agcatttgca ctaacaaaag ctttggcaaa tctaattgct tttaaatttg tcgaagccat
     5341 tgaacctaca aaagctccag ttatttctct atattcaccg ccaagaagca ataaatcaat
     5401 ggtttcgcta tcatttagaa tcaagaacac agggagacta tttgtaatga cacgaatttt
     5461 ataaccttta cgacctttca attctaatgc cagttgttca agtgtagttc caggtccaat
     5521 aaaaatagta tcgccatctt tgattaaaga gcaggctttt ttagcaatgt caattttttc
     5581 ttttgtattt agacttttct tctcgatatg tgtcttttca agtggctttt tagaggaaag
     5641 caattgtgca ccaccatgag tacgtgttaa aagtccatca gcttctagag catcaaggtc
     5701 tctacgggct gtcatatcgg aaatatctag ttcatctatt atttctttta tggttatggt
     5761 cccatcaatc tttaacatat ctaaaatttt ttctaatcgt cgttttttgt tcatatgaag
     5821 acccttcttt cataaagtaa tttttttcca aagataattc tcttttaatt gtatcataaa
     5881 agacaatatt ttcaaggtaa aacaaacaat ttcaaacaaa aacaaatgtt agatgatgaa
     5941 ataagaacag aggattgacg tatattagct taggtcagat tttgtataag acgaaaataa
     6001 agtaggacct cttaatcagt aagttataga aagtaaaaga cttttgtaat acttgaatag
     6061 atatttcacg tccattttgt gatggattaa atgaacaaaa atgaacaata atttaacggt
     6121 gttatctatt ttttaaaaaa acaaataaaa aaaacaaaaa ataaacaaaa aaagtgttgc
     6181 tttttgtttg aatgcttgat attatataaa caaagaaatg atgaaaacgt tttttcgaac
     6241 attttgcaaa atgttttcta cttctacgta gcatttcttt ctaaaattta ggaggtggtc
     6301 aaaatggcta ttgttgttgg tgcagatctc aaaggtacga gattaaaaga tgttgtgaaa
     6361 aattttcttg tagaagaagg ttttgaggta attgatgtga ctaaggacgg acaagatttt
     6421 gttgatgtta ccttggcagt tgcctctgaa gtaaataaag atgagcaaaa cttaggtatt
     6481 gtaattgatg cttatggagc tggtccattc atggtagcaa ctaaaattaa aggtatggtt
     6541 gcagcagaag tttcagatga acgctcagct tatatgacac gtggacataa caattcacga
     6601 atgattacgg ttggcgctga aatcgtcggt gatgagcttg ctaaaaacat cgctaaaggt
     6661 ttcgtcaatg gtaaatatga tggcggacgt catcaaatcc gagtagatat gcttaacaaa
     6721 atgtgttaag aaaaggagtt aaggaatgag aatcgcaatt ggatgtgacc acattgtcac
     6781 agatgaaaaa atggcagtat cagaattctt gaaatcaaaa ggacatgagg tccttgattt
     6841 cgggacatat gaccacgcac gtactcacta ccctatctat ggtaaaaaag tcggtgaagc
     6901 agtagtaagt ggtcaagcag acttgggagt atgtatctgt ggtacaggtg tcggcattaa
     6961 caatgcagtc aataaagttc caggtgttcg ctcagcgttg gttcgtgata tgacatcagc
     7021 tatatatgca aaagaagagt taaatgcgaa tgttatcggt ttcggtggaa agattactgg
     7081 tggtcttctt atgaatgaca tcattgaagc tttcattgaa gctgagtaca aaccaacaga
     7141 agaaaataaa aaattgattg caaaaatcga acacgttgaa acacataatg cacatcaagc
     7201 agatgaagat ttctttacag aattccttga aagatgggac cgtggtgact accacgatta
     7261 aggtataaca aatgattctg acagtcacac taaatccttc agtagacatc tcttatcctc
     7321 tagaaacatt gaaaattgat acagtgaata gggtgaaaga tgttagtaag acagctggtg
     7381 gaaaaggctt aaatgttact agagtcttgt atgaatctgg tgataaagtt actgctacag
     7441 gtttcttagg tggtaaaatt ggtgaattta ttgaaagcga attagaacaa tctcctgtta
     7501 gtccagcttt ttataaaatc tcaggaaaca ctagaaactg tattgctatc ttacacgagg
     7561 gtaaccaaac agaaattttg gaacaaggac caacaatttc tcatgaagaa gcagaaggct
     7621 ttcttgacca ctatagcaat cttattaaac aatcagaagt tgtgacaatt tcaggaagtt
     7681 taccttcagg acttcccaac gattattatg aaaaacttat ccaacttgct tcggatgaag
     7741 gagtagcagt cgttttggat tgttcaggtg cacctcttga aacagttcta aaatcgtcgg
     7801 ctaaacctac tgccatcaaa cctaataatg aggaactttc acagttgctt ggaaaagaag
     7861 taacaaaaga tattgaagaa ctcaaagacg ttcttaaaga gtcacttttt agtggtatcg
     7921 aatggatagt ggtctcatta ggtcgaaacg gtgcctttgc aaaacatggt gatgttttct
     7981 ataaggtaga tattcctgat atcccagttg ttaatccagt cggatcaggg gactcaacgg
     8041 tagctggtat tgcatcagct ttaaatagta aaaaaagtga cgctgactta ttaaaacatg
     8101 cgatgacatt gggtatgtta aatgctcaag aaacaatgac agggcatgtg aatatgacta
     8161 attacgaaac actaaactca caaattggag taaaagaggt ataaaaatgg tacttacaga
     8221 acagaaacgc aaatctttgg aaaaaatttc agataaaaac ggtgttatct cagctttggc
     8281 atttgaccaa cgtggtgctt tgaaacgttt gatggcacag tatcaagata cagaaccaac
     8341 tgtagctcaa atggaagaac taaaggtttt ggttgctgat gaacttacaa aatacgcttc
     8401 ttcaatgttg cttgaccctg aatatggact tccagcaaca aaagcattgg ataaagaagc
     8461 aggtcttctt cttgcctatg aaaaaacagg ttatgataca tcaagtacaa aacgtttgcc
     8521 tgactgcttg gatgtttggt ctgcaaaacg tatcaaagag caaggcgctg atgccgttaa
     8581 gttcttgctt tactatgatg tagacagctc agatgaactt aaccaacaaa aacaagctta
     8641 tattgaacgt gttggttctg aatgtgtcgc tgaagatatt cctttcttct tggaaatcct
     8701 tgcttatgat gaagaaatct cagatgcagg ttctgttgaa tatgcaaaag tgaaacctcg
     8761 caaagtcatt gaagcgatga aagttttctc tgatccacgc tttaacattg atgttcttaa
     8821 agtagaagta ccagttaacg ttaaatatgt cgaaggtttt gctgatggtg aagtagttta
     8881 cagtaaagct gaagctgctg acttctttaa agcacaagaa gaagcgacta accttccata
     8941 catctaccta agtgcaggtg tatctgctaa attgttccaa gaaacattgc aatttgctca
     9001 cgactcaggt gccaagttta atggtgtgct ttgtggacgt gctacttggg caggatctgt
     9061 agaaccttac atcaaagaag gtgaaaaagc agcacgtgaa tggttgcgta ctactggatt
     9121 cgaaaatatt gatgaactta acaaagttct tgttaaaaca gctagtccat ggactgataa
     9181 agtatagtaa aaacataaaa cggaggatat tgttatgaac agagaagaga tgactctctt
     9241 agggtttgaa attgttgctt atgctggaga tgctcgctct aaacttttag aagcgcttaa
     9301 agcggctgaa aatggtgatt tcgctaaggc agatagtctt gtagtagaag caggaagctg
     9361 tattgcagag gctcacaatt ctcagacagc tatgttggct cgagaagctt ctggggagga
     9421 acttccatac agcgttacca tgatgcatgg tcaggaccac ttaatgacta ctgtcttatt
     9481 aaaagatgtg attcatcacc tcatcgaact ttataaaaga ggagcaaaat aatgcataaa
     9541 ctcattgaac ttattgagaa agggaaaccg ttctttgaga aaatctctcg aaacatctat
     9601 cttcgtgcta ttcgtgatgg atttatcgct ggtatgccag tcatcctttt ctcatctatc
     9661 tttatcctta ttgcctatgt cccaaatgct tggggattcc actggtcaaa agacattgag
     9721 accttcttga tgactcctta tagctattcg atgggtatcc tagcattctt tgttggtggt
     9781 actaccgcta aagctttgac ggactcaatg aaccgtgacc tacctgcgac caatcagatt
     9841 aacttcttat ctacgatgct agcgtctatg gtagggttcc ttttgatggc agctgagcct
     9901 gcaaaagaag gaggcttctt gacagccttc acgggaacaa aaggtctttt gacagcattt
     9961 attgcagcct ttgtgacagt taatgtttac aaggtctgtg tgaaaaataa tgttactatt
    10021 cgtatgcctg aagaggttcc accaaatatc tctcaagtat ttaaagactt gattccattc
    10081 actgtagcgg ttgttctcct ttatggattt gaactcattg ttaagggaac tcttggtgta
    10141 accgttgcag aatcaatcgg tacccttctt gctcctctct tctcagctgc agatggttac
    10201 cttggaatta cacttatctt tggtgcttat gccttcttct ggtttgttgg tatccacggt
    10261 ccttcaattg tcgaaccagc aatcgctgct atcacttatg ccaatatcga tgccaacttg
    10321 catcttatcc aagctggaca acatgcagac aaagttatca cttctggtac tcaaatgttt
    10381 attgttacca tgggtggaac aggagctaca ttgattgttc cattcttgtt catgtggatt
    10441 tgtaaatcag aacgtaaccg tgccatcgga cgtgcctcag ttgttccaac attctttggg
    10501 gttaatgagc caatcttatt tggtgcacca atcgtattga atccgatttt ctttgtaccg
    10561 ttcattttcg ctccgattgt caacgtttgg atctttaaat tctttgttga cacattgaac
    10621 atgaactcat tctctgccaa ccttccatgg gttactcctg gtccgttggg aattgtactc
    10681 ggtactaact tccaagttct atcatttatc ttagccggac tcttggtagt tgttgatact
    10741 atcatttatt acccatttgt taaggtatac gatgaacaaa ttcttgaaga agaacgttct
    10801 ggtaaaacaa acgatgctct taaagaaaaa gtagctgcaa acttcaatac tgctaaagca
    10861 gatgccgttc ttggaaaagc aggcgttgct aaagaagatg tagcagcaaa caataatatc
    10921 acaaaagaaa ctaatgtcct tgttctttgt gcaggtggag gtacaagtgg cttgcttgcc
    10981 aatgccttga acaaagcagc agcagaatac aatgttccag tcaaagcggc agcgggtagt
    11041 tacggtgctc accgtgaaat gttgccagag tttgacttgg ttatcttggc tccacaggtc
    11101 gcttcaaact tcgatgatat gaaggctgaa acggataaat tgggcattaa acttgcaaaa
    11161 actgaaggag ctcaatatat caaattgaca cgtgatggac aaggtgctct tgcatttgtt
    11221 caacaacagt ttgattaata aatttgtaaa agttatttga gacgagatta ttctttcaat
    11281 tccttataat attgttaacc atatcattgc atttcatttc cttggtttcc ggaactcaga
    11341 atatcgtctc aaatgctttt tttgaaagga tttatagtta tgactaaaac acttcctaaa
    11401 gattttattt ttggtggtgc tacagctgct tatcaagcag aaggtgcgac tcacacggat
    11461 ggtaaaggcc cagttgcatg ggataagtat cttgaagaca attattggta cacagcagag
    11521 cctgcaagtg atttttacca taaatatcct gttgatttag aattagctga aaaatatggc
    11581 gttaatggta ttcgtatttc aattgcctgg tctcgtattt tcccaactgg ctatggcgaa
    11641 gtaaatgaaa aaggtgttga attctaccat aatctttttg ccgagtgcca caaacgtcat
    11701 gttgagccat tcgtgacttt gcaccacttt gacacaccag aagttcttca ctcagatgga
    11761 gatttcttaa accgcgaaaa tatagaacac tttgtagatt atgccgcttt ctgttttgaa
    11821 gagttcccag aagtaaacta ctggacaact ttcaatgaaa ttggcccaat tggagatggt
    11881 caatacttgg ttggtaaatt ccctccaggc attcaatatg atcttgccaa agtcttccaa
    11941 tcacaccaca atatgatggt ttctcatgca cgtgctgtga aattgtataa agataaaggt
    12001 tataaaggcg aaattggtgt agtacatgcg ttgccaacca aataccctta tgatccagaa
    12061 aatccagcag atgttcgtgc cgctgagtta gaagatatca tccataacaa atttatcttg
    12121 gatgcaacct accttggaca ctattcagat aaaacgatgg aaggggtcaa ccacatccta
    12181 gctgaaaatg gtggagaact tgatcttcgt gatgaagact tccaagcact tgaagcagct
    12241 aaagatttga acgatttcct tggtatcaac tactacatga gtgattggat gcaagccttt
    12301 gatggtgaga ctgaaatcat tcacaatggt aagggtgaaa aaggaagttc taagtaccag
    12361 attaaaggtg ttggtcgccg aatcgcacca gattatgtgc ctagaacgga ttgggactgg
    12421 attatttatc ctgaaggctt gtatgaccaa atcatgcgag tgaaaaatga ttatccaaac
    12481 tacaagaaga tttacatcac tgaaaacggt cttggataca aagatgagtt tgtagataat
    12541 accgtttatg atgatggtcg tattgattac gtgaagaaac acttggaagt tttatcagac
    12601 gcaattgcgg atggtgcaaa tgttaaaggt tacttcatct ggtcacttat ggatgtcttc
    12661 tcatggtcaa acggatacga aaaacgttat ggactattct atgtagactt tgacacgcaa
    12721 gaacgctatc ctaagaaatc agctcattgg tataagaaag tagcagaaac tcaagtgata
    12781 gagtaatatt acttgaaaat gtaaaaaatt gttatccgat actttagaaa aagtacaaca
    12841 cttatttaga ccaggctttg aaaaggtata tattgttagt ttcgaagatt gtcctatgat
    12901 tcctgagctt gaagctaccc cgttgctaaa atgtggtaaa tggtatgttt caactggaaa
    12961 agagtggatt tgccattctg atttagagtt atcagcattt gaatgggaat ttttacaatc
    13021 actcgatgta gaaatacgtg agactattca cttcgaggta aattatttgc ctttccaata
    13081 ataaaaagaa aggaattagt tgtgactatc gaattaaaaa acgagtatct tactgttcag
    13141 tttaaaacac ttggtggtca actgacttcg attaaagaca aggatgggat agagtatctc
    13201 tggcaagctg atccaaatta ttggaatgga caggcgccta ttctatttcc tatctgtggt
    13261 agtttacgaa atgattgggc tatttataga cctcaagaaa gacccttttt tacaggtctt
    13321 attcgtagac atggttttgt tcgaaaagaa gaatttattc tggaagaagt caatgataat
    13381 agcgtgacct ttagtattaa accaaatgct gagatgcttg ataattacct atatcagttt
    13441 gaactgagag ttgtttatac cttgaatggt aaatcaatta gaaccgaatt tcaggtaacg
    13501 aacttggaaa tggaaaaaac aatgccttac tttattggag cccatccagc attcaatagt
    13561 cctttagtag aaggtgaaaa ctatgaggat tattatattg aatttagtga ggttgagtct
    13621 tgctcgatac caaagagttt tcctgagaca ggattattag atttgcaaga tcggacacct
    13681 tttttggaaa atcaaaaaat tctcgatttg gactactcac ttttttccca tgatgccatt
    13741 acacttgacc agctcaagtc tcgtagcgta acgcttcggt caaggaagtc tggtaaaggt
    13801 cttcgagtag attttgatga ttttccgaac ttgattctgt ggtctacaac taataaaagt
    13861 ccattcattg ctttggaacc ttggagtggt ctctccactt cacttgaaga aggtaatttc
    13921 ttagaagata aaagacaggt aacgaaaatt tcacctcaag aaacttctag aaaaatctat
    13981 gatattacta tccttaatta aaatttgtta ttatttgagt cttcaataag atttgaatac
    14041 tcttaccttg tgatgataga ttggtcttct ccgtgccagt cttacatcgc aaggttttta
    14101 agttttttgt gggtctatgt caactgtagt gggtgacgaa aagctaatat ctaaagacag
    14161 tctggtagta aaaaataaga ttatttacta cttcttgatt taaaataata gtggagccat
    14221 taatttgtgg caaatttaat ttaatatttt gcctcaaatt agccacactt tttatagaac
    14281 taagaggaaa aatgaaattt tcttcctatt aatatagttg tttgaaatct taaatatagc
    14341 gt
//
LOCUS       DARBFO010000067        13810 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000067 DARBFO010000000
VERSION     DARBFO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.068"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(221..1423)
                     /locus_tag="SCK51_002085"
     CDS             complement(221..1423)
                     /locus_tag="SCK51_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1252700.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="SCK51_002086"
     CDS             complement(1426..1620)
                     /locus_tag="SCK51_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG1252701.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="SCK51_002087"
     CDS             complement(1667..2515)
                     /locus_tag="SCK51_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HEG1252702.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="SCK51_002088"
     CDS             complement(2720..3070)
                     /locus_tag="SCK51_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252703.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="SCK51_002089"
     CDS             3665..4111
                     /locus_tag="SCK51_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEG1252704.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="SCK51_002090"
     CDS             4735..5727
                     /locus_tag="SCK51_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1252705.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="SCK51_002091"
     CDS             5743..6627
                     /locus_tag="SCK51_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1252706.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="SCK51_002092"
     CDS             6676..8283
                     /locus_tag="SCK51_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1252707.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="SCK51_002093"
     CDS             8284..9768
                     /locus_tag="SCK51_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEG1252708.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="SCK51_002094"
     CDS             9945..10937
                     /locus_tag="SCK51_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1252709.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="SCK51_002095"
     CDS             10989..11858
                     /locus_tag="SCK51_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1252710.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="SCK51_002096"
     CDS             complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="SCK51_002096"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEG1252711.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DARBFO010000068        13398 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000068 DARBFO010000000
VERSION     DARBFO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.069"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(14..706)
                     /locus_tag="SCK51_002097"
     CDS             complement(14..706)
                     /locus_tag="SCK51_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG1252712.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="SCK51_002098"
     CDS             complement(1143..1712)
                     /locus_tag="SCK51_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1252713.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="SCK51_002099"
     CDS             complement(1786..2109)
                     /locus_tag="SCK51_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252714.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="SCK51_002100"
     CDS             complement(2263..3075)
                     /locus_tag="SCK51_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252715.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="SCK51_002101"
     CDS             3429..4277
                     /locus_tag="SCK51_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEG1252716.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="SCK51_002102"
     CDS             4422..5363
                     /locus_tag="SCK51_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEG1252717.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="SCK51_002103"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="SCK51_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="SCK51_002104"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="SCK51_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEG1252718.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="SCK51_002105"
     CDS             complement(9827..12013)
                     /locus_tag="SCK51_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEG1252719.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="SCK51_002106"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="SCK51_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEG1252720.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="SCK51_002107"
     CDS             complement(12235..12675)
                     /locus_tag="SCK51_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEG1252721.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="SCK51_002108"
     CDS             complement(12749..13288)
                     /locus_tag="SCK51_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1252722.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2380 g         4446 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaataa
//
LOCUS       DARBFO010000069        13234 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000069 DARBFO010000000
VERSION     DARBFO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.070"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            169..1038
                     /locus_tag="SCK51_002109"
     CDS             169..1038
                     /locus_tag="SCK51_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HEG1252723.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1247..2272
                     /locus_tag="SCK51_002110"
     CDS             1247..2272
                     /locus_tag="SCK51_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEG1252724.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2311..3261
                     /gene="manA"
                     /locus_tag="SCK51_002111"
     CDS             2311..3261
                     /gene="manA"
                     /locus_tag="SCK51_002111"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEG1252725.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3355..5058
                     /locus_tag="SCK51_002112"
     CDS             3355..5058
                     /locus_tag="SCK51_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1252726.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5060..7093
                     /locus_tag="SCK51_002113"
     CDS             5060..7093
                     /locus_tag="SCK51_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1252727.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7104..7544
                     /locus_tag="SCK51_002114"
     CDS             7104..7544
                     /locus_tag="SCK51_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1252728.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7744..8871)
                     /locus_tag="SCK51_002115"
     CDS             complement(7744..8871)
                     /locus_tag="SCK51_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1252729.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8874..9551)
                     /locus_tag="SCK51_002116"
     CDS             complement(8874..9551)
                     /locus_tag="SCK51_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1252730.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9815..11581
                     /locus_tag="SCK51_002117"
     CDS             9815..11581
                     /locus_tag="SCK51_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEG1252731.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11596..12033
                     /locus_tag="SCK51_002118"
     CDS             11596..12033
                     /locus_tag="SCK51_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1252732.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12046..13197
                     /locus_tag="SCK51_002119"
     CDS             12046..13197
                     /locus_tag="SCK51_002119"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEG1252733.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4494 a         2300 c         2732 g         3708 t
ORIGIN      
        1 aacagctttt tcacaacctc tttttataag agtttttact cattaattta caaaaaacaa
       61 gcaaaatgcg aattttctca catttttttt aacaaaatca aagttattgc ttgaaagaag
      121 aagaatccat cgttaaaata aagcttgacg tagaggggag caagaaatat gaaagatttt
      181 caattggggg ctgccaccta tttaaaaagt gtagccattc gggtgaaaga ccgagataaa
      241 atgattgatt tttacaagaa cgttgttggc tttgatttga aaagagaaga aaacgaatta
      301 gccattttag gaacagaaga agctggaagt gaaatgttgc tgctggaaga aagtcctaga
      361 gcgaatgatc acatgggggt gatcaaaaag ctgaaccgtt tttcattgat tatccctagc
      421 acagaagaaa tggcagatat cctttgtcgt atccgtaaaa tgaattatcc aatcaaagaa
      481 gctttagaag atcatggacg tatggggatt ttgctcgtag acccagaaga gaatgaattg
      541 gaaatttacc atgaagagaa taagaacgac caaagagaaa ttccagaaac aatggatcaa
      601 gaagcattat taagaaaatc tgaagaaaaa ttcagtaaac tatcaacagg ttcttatttt
      661 gaaaaaatcc atttaaatgt tactaactta acaaaagaac gccaatttct taatcatgta
      721 cttggtttga aggtacataa tgaagaagag ggcatccatg tattgaatga aggagatttc
      781 catgttggtt tgacagaagc tagcggaggc gcaattgatt tgcctacaga tgaagtgatt
      841 ggacttgatt ttctgaaatt catgatcgat actgaaagca tggaaggact gattgctcac
      901 ttgcagcaac tatcagttga tttcttctta gacaaaaagc acacagtact cacaatctat
      961 gatgctatag gaatcgaatg gtggttcgtc agtgagccaa aagaaaagaa taaaactgac
     1021 attcgatgcg acgcttaact ttttctttct taacgattgt cgcatgtctc gtcattttat
     1081 gaacggtcat actatcgctt attctagaga tagaaagaga aaatagtata ccgggggaaa
     1141 ctgcgcggtt tttcgcgcag tttttttgtg taaaaaaggt tgattcctca tagattaagc
     1201 tttaagcttg gactgtaaga atctgataga aaagcaggag aaaaagatgt atccacacac
     1261 gaaacagctt attttgaaaa agatggaaga aggattttat ccaggtgtag cagtcgcctt
     1321 cataaaagaa aatcaagtag aaacatttac agccggcttt gcccaagtcg ttcctgaaaa
     1381 gcagctgatg acagaagatc tgttgtttga tgcagcctca ctgacgaaag tcgttgcaac
     1441 cacttcatta gttttacgtc tattagaaga tggaaagata gaactcgatc aaccgcttca
     1501 taaatatctt cctgaatttg aaaatcagac aatcactttg cgtcatttac tgacacatac
     1561 ttctgatatc aaaacatgga taccaaacag agataagcta tctcaggaag aattgatcgc
     1621 cgcttatttg aagctagaac caggtgagca actaggaaaa gaagtaaaat atacagatgc
     1681 cggtacgatt cttcttgggt tgatgctaga ggaaatttac caagaaactg cagtggaaat
     1741 ctttcaagaa caagtattgg aaccattagg aatgatgaac agcttatttc ttccttttcc
     1801 ttccaaaaaa atcgttccca cagaacaact gccttcgggt gaggttttgc gtggacaaac
     1861 tcatgatccg aaagcaagag ttttggctga acatgcaggt aacgcgggtc tctttattga
     1921 tttgaaagac agcattactt ttgtccaaat gtatttgaac aagggaaaaa tccgtaatgg
     1981 tcgtttttta gaagaagaga cgattgattc tttactgcaa gatcaaacac cgaaaaaaaa
     2041 tggctatcgt tctttaggat gggacttaaa aaaggatgta gaagacggtc atgcgattct
     2101 ttttcatact ggttatacag gtacgttttt cttgattgat ataaaaacac aagaagcgtt
     2161 gatatttttg tcaaatcgcg tacatcctgt cgatcatcgt gcggagtatc tcatatgtcg
     2221 agatgaaatt gttgaaaact ttttgaaaga gaaagcggta ttaccgaaat aatgctataa
     2281 tagctatgaa aaatgagagg agtttgaatt atgcaggaac ctatgttttt aaaaccagtt
     2341 tttcaagaaa aaatttgggg aggaagtcgt ttacgttcag tctttggttt tgatatccct
     2401 aacgataaaa tcggagaaga ttgggcgatc agtgcgcatc ctcatggtgt cagtgtagta
     2461 gaaaatggcg aattcaaagg gaaaagatta gatgaactat ggtcagaaca caaggaattg
     2521 tttggccatc cttcagaacc agtgtttcca ttattgatca agatcttaga cgcagaagat
     2581 gatctatctg tacaagtaca tccagatgat gcctatggga tgaaacatga aggtgaatta
     2641 ggaaaaactg aatgctggta tatcatcgat gctgaacctg gtgcagaaat catctatggt
     2701 catcatgcaa agacaagaga agaactagct gagatgatca aagatgggcg ctgggatgac
     2761 cttttgaaga aagtaccagt taaaaaaggt gatttctttt atgtaccaag cggaacgatc
     2821 catgctattg gtaaaggaat catgatttta gaaacacagc aaagttctga cacgacttac
     2881 cgcgtgtatg actacgacag aaaagatgcg caaggacaaa caagagagct gcatatccaa
     2941 caatcgatcg atgtaacaac tgtccctgcg aaaacaccag aattacaaat caaagaagtc
     3001 cgaaaaggga attcatcgat cgtcacttat ttagaaactg agtttttcaa tgtatatgag
     3061 tgggatatta aaggaatcac atctttcaaa aaacaagccc cttatacatt gatgacggta
     3121 atcgaaggag caggagacct tgtcgttgac ggaaaaacat atccattaga aaaaggaaca
     3181 agctgtatta ttccaaatgg cgtttctgag tgggcggtcc aaggtgaact agcaatcatt
     3241 gcgactgaac caggcaaata gttcccagat agctgaaagc agcagacggt ttttcgtttg
     3301 ctgctttttc tacataaaat ccgtccatca ataaatagaa gtatggtgaa aaagatggaa
     3361 tacaaatgga aagcaaacaa cagcagtcgc gtgtctatga aacaactgag aaaagctgga
     3421 catcacttaa atgaaatgat catgccaaat ctaagtctgt ttatcgcttg gggaatcttg
     3481 tcattagcaa caaaaggtgt gtctggcgat ttgcacacca cattagaaga ggtatgccat
     3541 tggatgatcc agctgctttt acccatcttg atcagttatt caggcgggaa atcggtaggg
     3601 ggacaaaaag gggcagtcgc cggtgcaatt gcttctttag ggttgatcgc tcgttccaat
     3661 gctccacaaa tcgtaggagc gatgattatc gggccgcttg ccggcatcgt atatgaacaa
     3721 tttgtttcta gattccacgt gaaattcaag gctggatatg aaatgcttgt cagcaaccta
     3781 ctagtaggac tgactggtag cgttatttgt ataattggaa tcgtttttat tgaaccagtt
     3841 ttaaatagtg ttcacttgtt gctcgcctcc gttgtgtctt ggctagtcac aatgaatctg
     3901 ttaccacttg tcagtctgat cttagaacct ctaaaagtac tatttttcaa taatgcaatc
     3961 aatcatggtg tattgacacc tttaggaatc gaattctcac aaacagtcgg gtactcttcc
     4021 ttatttttga tggaggcaaa tcctggacct ggtttaggga tcttactttc tatcctttgt
     4081 tttgcagaaa aacaggaaaa agtaaatgcg agtggagcat tgatgatcca tgcgattggc
     4141 gggattcacg aaatctattt tccatttgtc ttgctgcgac cgtatctatt tttagctgta
     4201 atggctggtg gggcaagcgg cacgttgata tttcaatggt tagatacggg attagctgca
     4261 cctgtatcgc ctgggtcact actcgtgatt tttgccaatg cgccttatcc acaactagct
     4321 ggggtaagta tagggatagc aatcagtaca tttgtgacct tcatttgtgc agcgatacta
     4381 ttgaaattac caaataaaga aaaagagaac aaaacggtca aagaaagggg agaaatcttg
     4441 gaaccatcta tcattcattc cgtcattttt gcttgcgact caggtatggg atcaagtgct
     4501 atgggagccg ccctgctgaa aaagaaatta gcagcagaaa aaatcgatct ttctgtcgga
     4561 tacagctcta tctatgaatt gaataaccaa gtaaatcagc tggttatcgt gcaacatgaa
     4621 ctagcgggtc ttgcaagaga aaaagtacct caaagtcaaa tctatgtact ggatcacttt
     4681 ttggaaacag atatcttgct gtcacgaata aaagaagcga tccaatttcc aaagcgggaa
     4741 gaaaaaaaag agatagcaga tatcacagaa caaaaaaagt cgcctgctaa tattgtattt
     4801 ttgtataaaa acaacagacg cggctcacaa actatgggca tgacgatttt aagtcaatta
     4861 gcaaaaaaag cgaaaaaaca gtttgttatc tcaaaagaat caatagaaaa aatcaaaatc
     4921 caagaagaaa cgatttatat cgcacaaaga gaactagctg taactgaaca gctagaagaa
     4981 aagatccctc ggctagttct agttgaacat tgggtagcta cgaatgaata cgaaaaactc
     5041 gtgaagggag aaatataaga tgtatttatc gcccagagaa aaacaattat tagcagaatt
     5101 tctcagtagc ccgtcacctg tatctatcca aaaaatgatg aatttattga aggtcagtaa
     5161 acggactatt taccgcgaat tagataatct tgaagtctca ttgaaatcgg taaatgcttc
     5221 tttgaaaaaa gcagctagag gctcatttgt cattgaggca tcagaagaag tattagagca
     5281 attaaaagaa aatacagatg ggaaaagtaa tgacttatcc actgctaaac gacaacacgc
     5341 gattttagcc gagttgttac tgacgacaga gccgttgagt cttgctcatt tcatggaatc
     5401 atatcgcatc agtaatacca ctttttatgc agatatcaaa cagttggaag aaagtattcg
     5461 ccaactgcct ttggaaatca ttcggaacca gggatacgag atcgaaggac cggaaaaata
     5521 ccgccggctt ctgacagcga atgtattaga attagaaatc aatgaatatg aactttttca
     5581 tagtatttct ttcgatgcat cactgaatta tttttttcga ttcgttgatc cgcaacatct
     5641 atctcttgca cgcaaagtaa tcggggaaga attgatccaa aaaaagacca atctaagtga
     5701 tagaaagctt gaacatttgg ttttgatgct gacattgacg atggagcgtg tgactaaaaa
     5761 tcatttactg acaaatgaga catataatag tctggcaaac aaagaattac tacagacagc
     5821 taaacgattg ttttcacgga tcgcttctga gacaaaacaa ctttatccag taaatgagat
     5881 cgtctttttt gcaggattat taaatgactt cacgaactca tttgatcaga atttctttga
     5941 agagacgttc gatacacagc ttgcttattt agtaaaacaa ctgattcaag aagtaagtga
     6001 agagacgaat gtccattttt atgaagatga aactttgtac aaaatgctgt tgactcattt
     6061 atctggtgtg ttttcaagag ccgttttaca agaagagaca ctgaccaatc cgattttaga
     6121 aaaaatcatg gttcaatacg aggaagttgc gaatgcaatc cgtaaagcag tagagcggat
     6181 ttttcaagaa aaaaaattat ccgaagaaga gatcgcttat atggtccttc attttgcgaa
     6241 ttcattggaa cgaagtccga aagaaagcaa tatcagtatt gcaggatttt cgccaagtgg
     6301 attagcttct actagcatgt tggaactacg attgaaaagg tattttccct tcatccaaca
     6361 aattcatttc tatcggattg ctgatttaaa aaaaatcgat ttacaagaag aatatgatgt
     6421 tgttatttct acatctattt taccaggata cggtggaaaa tatattttag tctccccgct
     6481 actgctagaa gatgaagtca agcagctaaa agaagctttt tcgcatattg agaagaaaaa
     6541 aaggcaaaaa attacgtata acagcaataa cgagtggatg atagaagata cttatgagga
     6601 aacaatgcat ttcatggatc aaatcaatca attactcggt cgttttttta tccaagaact
     6661 ggataatcct gagacgattt ccgaaacgat tcgcagctta tttccttatt ttcctgaagg
     6721 actagtaacg gatgaagagg tggttcatcg ccggctgctt aagcgatatg aacaagcacc
     6781 tatcggtatt cctcatacaa atatggggtt attccataca tccagctcat tgatttctaa
     6841 accgatcttt tgtattttca atttgaaaca tccgttgacc attgaaggga tggatagaca
     6901 gccagtcgat ttgtcccgta tgttagttat gctggcgcct tcgccaatcc aagaaaaaga
     6961 cgggattctt ttaggaaaga tcagtggagc aatcatcatg aatgacttga acaccgagat
     7021 cttccgctca ggaaataaag aaatcgttta tcagttgtta gcgaaaatcc tgattgaaga
     7081 aataaagaaa tgaaggtgaa aagatgatac agctaccagc agaaaaaata ttgctcgatc
     7141 aatcctatca aaattggaaa gaagcgttga ccgagatcgg ccagtggatg acccaacaag
     7201 ggggaatcaa accggcatat atccaatcaa tgatcgaaag acaagaaaaa gcgagtgtct
     7261 atatcgggaa tttcgtagcc ttgccacatg ggactgacaa agatgaagaa gtagtagaag
     7321 aaggaatcta tttagtccaa gtgcctgatg gtgtggactt tggaacaaaa gaggaaccaa
     7381 aaatcgcgac actcttattt gtagtggctt taaaaaaaga acatcagtta gcggtgctcc
     7441 aagatttagc ctttttctgt tcagacatcg accaagtaat ggcgctcagt gatgcgaaaa
     7501 ccattgaaga agtccaaatg atttttgaac atggacatat ataaccaatg ttcacagtaa
     7561 aaaaagctgc cttgcatcaa gtaataactt caaccaagat aaaaataaga ggagctattt
     7621 ttgttgggag gttcttatta ctgcggatgc aagcttttga aaactattta ttctagaaaa
     7681 ccgtttattt gaaatagggg acggtgacaa ggtttttgtc accgccccta ttatttttgt
     7741 gttttaatag ctctttttta tatagatagt gcccatacct atgaataata aaacgatact
     7801 ccatatacca agtgtcaaga taccattcat gaaagtaacc tgttctgaag ctagtggcga
     7861 gaaaatatct cctcctatga tcacattttg aaaatcaaca taagaagttg gcaagtaatg
     7921 actgattgat tctatccatc caatcgaaga aaacaagcga tactctgcta agaaaagaca
     7981 aagaagtgta cctaaaagat tgactagaga atgttcaaat aaagcagata gaagaaaact
     8041 gattgtactt aaaaagagca cccacaacaa caggaacaga aagctctgga aaagatactc
     8101 atacatcgga aggattcgta tctcctgacc aatgatcgtt cctacaggat aacggaagtc
     8161 tcccaaacca tttttgatta ttactagtat agagacgatc aaaaatggca tcacgagact
     8221 tagcatagct gcaccataat aaatggtaaa acggatgcta tgtttcttta ccatactatc
     8281 cggtaataga ttgactagag agattacctg ctttcttttt tcataagaga tgatggaaga
     8341 aagaaacaag gcggttatac ataaaatcaa aaaactagaa atcattcctg aaaaaatatt
     8401 ttcgtagtaa tttgctaaag ggagatgcgc tggcaagtca aaaatagaat atctttggat
     8461 ctgatgttct ttcatataac ggagcaactc gacacgtttt tgttgttcaa tgattggaat
     8521 tccttgtctt tgaccagaaa tcagtcgatc cagtatatct ttttcataat gataagttgc
     8581 ttcgaccatt tgctggacat tccctgtgtt ttttgcatgt aagattgctt cgatcagttt
     8641 gttgcttttc tctaaacttt ccacaaattc tttctcattc tcatcttctt tcaacgtttc
     8701 aatcaaacgt tgcatctctt catagttatg attcagttca tcgaattctg tataagcaag
     8761 tttatgatca aaaaaaagtg ggaatgcaaa gagcacgacc aacaatagag gaaataaaaa
     8821 gtttactttg ttttttagta agagattgcg ttcaaatgta agtaaagaca tagttacacc
     8881 tcatataatt tacgataaat agctaaaatc tcttccatat ttgaggattc tgtgacgatt
     8941 tctccatttt ttagaaaaca tgctttagaa caaatcgatt ggatattcgc gatagcgtgg
     9001 gaagaaagaa gaatcgtctt cccctctttc tgccattgtc tgatgaggct attcaagata
     9061 tcaatacttc ctgggtctaa tccgttcaac ggttcatcga ataacatgac agatgaatca
     9121 ctgatcaagt aagcagcaag caagacatgt tgtttcattc ctagagacat ttttccaatc
     9181 ggcaaagtaa ggtactcagt catcttcaat cgttggaaaa cagtttggca ttctactttg
     9241 ctctgccaat agtatttgat caaagtcaaa taatcaacag ccgatagata gtcggataac
     9301 tgattcgttg attctagaaa aaagaaagac cggcgataat ctgtataagc agtaggagag
     9361 atgttgtgaa gggacaattg atcataagtt gttggcagca gttgggcaat gccattcagc
     9421 aaagttgttt tgccaaatcc gttcggggca atcaaagcag ctacttctcc tgattctact
     9481 aaaagattca attgtcgaat cacacgtttt ttaccacgtt tcatgttcat gtttttgatc
     9541 agtaatgtca tagataggcc tccaatataa aatttaataa cataatagaa tggaaaacat
     9601 ctaaattaaa tgctaacaaa agttattgtt tgcatattat aaaaaaatta aaataacagt
     9661 tttatttaaa attgaagttt tctgataccg aaatcttttt attttgctga catactcaaa
     9721 cctttggcac aaagtttcgt gccaaagtac gactatttct ctttttagta agcggataca
     9781 attagagtat caaaaggaga gagggagaga caaaatggaa cggacgattc ccaaaaacaa
     9841 catttcgcta aaagcgaggg tacaaaaatt aggcagtact ttatcaagca tggtcatgcc
     9901 aaatattgga gcactgattg cctggggtgt attaacggct ctatttatcc cagatggtta
     9961 tttaccaaat gaggctttgg ctaccatggt aagcccaatg ctaacttatc tgattccttt
    10021 attgatcggt tatactggcg gaaaagtaat tgctggtgac cgcggatcag ttgttggggc
    10081 aattgcaacg atgggtgtga ttgtcggaac agatattcca atgatgctag gcgcgatgat
    10141 catgggacct cttggtggat atgccatcaa gaaatttgat cagctattcc aaaaacggat
    10201 caaatccggt tttgaaatgc tagtcaataa tttttcagca ggtcttatcg gttttggatt
    10261 agcgttatta ggtttttctg cgattggtcc agttgtcgat gcattgactc aagcaatggc
    10321 aaaaggtgta gagatcattt tatctgccca tttgattcca ttgacaagta tttttattga
    10381 accagcaaaa atcttatttt tgaataatgc aatcaatcat gggattttga caccattggg
    10441 aactgaacaa gtagcagagg ctggaaaatc tattctattc ttgcttgaag caaatcctgg
    10501 tccaggactt ggcgtattac ttgcttttac tttgtttggc aaaggagcag caaaatcttc
    10561 agcaccagga gccatgatca ttcacttttt aggcgggatc catgaaatct attttcctta
    10621 cgttatgatg aaaccgttat tattcttatc agttataggc ggaggaatgt caggaagttt
    10681 cattttccaa ttgatggatg ctggactaag agcgccagct tctccagggt cgatcattgc
    10741 gattctagcg atgacaccgt tcaattctta ccttcctgtt attttgggcg tgattgttgc
    10801 cgcagctgtt tcgtttggta tttctgcagt aattttgaaa gcggatgcga aagatacaaa
    10861 tgaagacttc gaaaaatctg ttcaagagac aaaggcagct aagcaagctt ccaaaggtgc
    10921 ccaaggtatt cctcaaggag ctacattctc tggcgtacaa aaaatcattt ttgcttgtga
    10981 tgcaggaatg ggatcaagtg cgatgggcgc ttcgatcttg agaaaaaaag tacaggaagc
    11041 agacttgcct caaacggtga ccaatcaagc aatcagccaa ctgacagatg actcgaatac
    11101 attgatcgtt actcaggtcg aactacaaga aagagcaaaa caaaaagcac cgagcgcgcg
    11161 ttttgtctca gtagaaaact ttttgaattc acctcgatat gatgaaatca ttcaagaatt
    11221 aagcagcaag cagaaaacat cagtttctga aattgagaat gagccagcag aagagtttct
    11281 ttcagcaaac atcaaacaaa tcaaagagat tctacttgtc cacgatgatc gttccggatc
    11341 agcaacaatg gggatgaccg tgctcaaaga tatattagac aagaaccatt taactatccc
    11401 gttaagaaaa gtcaatatcc aagatttaaa agatgaacct gattcactga tcgtagccaa
    11461 agaaagttta gcggaaaaag tacagatggc tgcacctcaa tccattcatt tatcagtcaa
    11521 cagcatggtc aatccgcaaa aatacgaatc gattgcacaa tatttgaaac agacagctta
    11581 agggaggaaa acagcatgca aagtttgcca ttcgacatga tcgagctcaa tcgatctttt
    11641 aaaaccaaag aagaagcgat ccgctattgc ggtcagatgc ttgtagaagc agggtgcgtt
    11701 gatgaggaat atatcgatgc catggtagat agagacaaaa tgttatctgt ttatatgggg
    11761 aattttatcg cgattcctca tggaacagat gaagcaaaaa aatatgtgaa aaaatccggc
    11821 atctgtgtcg ttcaagtacc agatggtgta gattttggta ccgaagaaga agaaaagatt
    11881 gcgactgttt tgttcggtat tgcaggagtc ggagatgagc atttgcaact tgtacagcaa
    11941 atcgcacttt attgcagtga tatggacaat gttgtccagc tagcagacgc gctcagcaaa
    12001 gaagaagtaa cggaaaatct agcaattgct taaggagaga gaaaaatgag agcagtacat
    12061 tttggtgcag gaaatatcgg tcgaggattt attggagagg ttttggcagc taatggattt
    12121 gagattactt tcgtagatgt gaatcacacg atcattgatg ctttaaatga aagaaaagaa
    12181 tacgaaatcg aattagcaga tgaatcaaaa gaaaaaatca ctgtaaaaaa tgtcaaagga
    12241 atcaacaatc aaacaaatcc agaaaaagta atccaagaaa tcgctgaagc agatttagtt
    12301 actaccgcca ttggcccgaa tatcttacct tttattgctg agttgattgc aaaaggtatc
    12361 caagaaagag aaaaggaagg aagcacaaca tctttagata tcattgcctg tgaaaatatg
    12421 attggcggtt cagcatttct agaaaaagaa gtgtataaat atcttccgga aacatcattt
    12481 actgacaaat atatcggctt tccaaatgca gcagtcgatc gaatcgtccc attacaacac
    12541 catgaagatc ctttattcgt ccaagtggaa cctttcaaag aatgggtcat tgacgatagc
    12601 caaagaaaaa ataaaaaaat ccaattaaaa ggggttttat atgtagatga tctagaacca
    12661 tatatcgaac gcaaactatt tagtgtcaat actggtcatg cgactgtcgc ttatacaggt
    12721 gccttgttag gttaccagac cattgatgaa gcaatgcaag atgcgttagt tgtggcacaa
    12781 ttaaaatcag ttttgcagga aacaggaagt ttgctgattg ccaaatgggg gtttgatgca
    12841 gagcagcatc atgcgtatat tgaaaaaatc attcatcgct tccaaaacaa aaatatctct
    12901 gatgcgatca cacgtgtcgc tcggacacca ttacgtaagt taggttatca agaaagattt
    12961 acacgtccag tgagagaact acaagaacat aacttgactt gcccacattt gacggcaaca
    13021 atggggatta tttttaatta ctatgatcct gaagatgaac aaagccgtca attgcatgaa
    13081 atgaagatcc atgaaaactt ggaacaattg attcaagaag tcacaggaat caatgatccg
    13141 aaaacaatcg ggaatatcaa gcaaaacgtg aaccgttatg ccaagcaagt cgcttaaaat
    13201 aggatacgaa aaaggggaat gtgacgaaat tttt
//
LOCUS       DARBFO010000070        13226 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000070 DARBFO010000000
VERSION     DARBFO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.071"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            115..1854
                     /gene="yfcC"
                     /locus_tag="SCK51_002120"
     CDS             115..1854
                     /gene="yfcC"
                     /locus_tag="SCK51_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEG1252734.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2011..3195
                     /gene="iadA"
                     /locus_tag="SCK51_002121"
     CDS             2011..3195
                     /gene="iadA"
                     /locus_tag="SCK51_002121"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEG1252735.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3306..3509
                     /locus_tag="SCK51_002122"
     CDS             3306..3509
                     /locus_tag="SCK51_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEG1252736.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3610..4272)
                     /locus_tag="SCK51_002123"
     CDS             complement(3610..4272)
                     /locus_tag="SCK51_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1252737.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4285..5787)
                     /locus_tag="SCK51_002124"
     CDS             complement(4285..5787)
                     /locus_tag="SCK51_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1252738.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6335..8014
                     /locus_tag="SCK51_002125"
     CDS             6335..8014
                     /locus_tag="SCK51_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEG1252739.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8141..8620
                     /locus_tag="SCK51_002126"
     CDS             8141..8620
                     /locus_tag="SCK51_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEG1252740.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8839..9519
                     /locus_tag="SCK51_002127"
     CDS             8839..9519
                     /locus_tag="SCK51_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEG1252741.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9527..10858
                     /locus_tag="SCK51_002128"
     CDS             9527..10858
                     /locus_tag="SCK51_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1252742.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10873..11835
                     /locus_tag="SCK51_002129"
     CDS             10873..11835
                     /locus_tag="SCK51_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEG1252743.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11946..12284
                     /locus_tag="SCK51_002130"
     CDS             11946..12284
                     /locus_tag="SCK51_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEG1252744.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12363..13070
                     /locus_tag="SCK51_002131"
     CDS             12363..13070
                     /locus_tag="SCK51_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEG1252745.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4199 a         2335 c         2903 g         3789 t
ORIGIN      
        1 aagtgatttt ctaataaatt attcatgtgg aaatgtcatt tttactgtat aattcatttg
       61 attatccatt ttttattcat caaaattact gaaaaaagaa aggaagaaat aaatttgaag
      121 aaaacaaatt cttcaggaaa accgaaacgc gacttcacta aattatcgac accccatacg
      181 tatgtcatta tttttggtgt agtcatcttt gcatggatac tgacttttgt cgttccagca
      241 ggaaagttca gcacgcaaga tattgaatac aaagatgcaa atggcgaaac aagtactcgt
      301 actgttttac gtcaagattc cttccgttat gcttacgagc tggataagtc gtatgtgttt
      361 gatcaattag aagaactgca agatcatcca gcagaacgtg aaaaactgga tgttccggaa
      421 aaaggattgg aaaaagtcat tgctgacgga gaaaaaaatc ttacgcagga aaaactagat
      481 gaaatctcct tgacagatga tgtgctttac gatcagtacg gcgaaaatat ctatgatact
      541 tctaaaaaat tgcataaaac agccaaaata tggggaacag atgattttgg cggctttggt
      601 ttcttaaatt ttgtttttga aggactagta tctggagata agtatggatc tgctgtaggg
      661 atcgctgcat tgatccttgt tgttggaggt gcttttggta ttattatgcg taccggagcg
      721 atcgacgcag gtatctatgc gtttatcagt aaaactaagg gactggagcg tttagcttta
      781 ccgctattat tctttgcttt ttcattcggt ggcgctacat tcgggatggc agaagaggta
      841 attccattct caatggtgat ggttccattt gtcattgcgt taggatatga ctcgatcgta
      901 gcagtaaccg tgacttatgt cgcatctcaa gtcggaaatg cgacctcttg gatgagcccg
      961 tttagcgtag cggtggctca agggattgct ggaatccccg tgttgtctgg tgcgacattc
     1021 cgattgatta tgtgggtagt tgttactgca ttagccgctg gttatatgat gatatatgca
     1081 gaaaaaatcc gtaaaaaacc agaaagttcg ttgacttaca aatcagatga ttatttccgt
     1141 tctcatatca aaaagacatc tgatgaaaat aaaaaattca tgcttgggca taaattgatc
     1201 ttattagaga tgttagttgt ccttgtctgg atcgtttggg gcgtgactca aaaaggctat
     1261 tacattccag aaatcgcttc tcaattcttt gtgatgggat tagttgctgg aatcattgct
     1321 gttcttttca aattagatgg aatgaaagtc aatgacatcg cttcttcttt ccaatctggt
     1381 gcagctgatt tagctggtac agcaatcgta gtaggtatgg ccaaagggat tcttcttgtc
     1441 ttgggaggat ctgatgcatc tgttccttca gctttgaata cgattttgca tgggatcggg
     1501 acggcgttga ccggtgttcc agctgtcatt ggtgcttggg cgatgtacat cttccaaagt
     1561 ttgttcaact tggttgttac atcaaattca ggacaagcag ctttgactat gccaatcatg
     1621 gctccactgg cagatttagt aggtgtatcc agacaagtag cagttcttgc ttatcagctt
     1681 ggttcaggtt ttatggacgc ttttacaccc gtatcagcca gcttgattgg tgtgctaggt
     1741 gttgcacgga tcgaatgggc aaaatgggca agattccaaa tcaagatgca aggattcctg
     1801 tttgttttag gaactatttt catcatgatt gccattgcaa taggtctgca gtagtactgg
     1861 agacctgtaa ataaaaaagt aatccaaaca gtagataagg actccaagaa ccgtcatttt
     1921 acggtaaaat caaaggagta aagatcctat cgtttggatt attttttaag atcctatcat
     1981 cagttatact taaagaaaga ggaaaaatgt atgaaaatca ttcgacaaat tgaagtgttt
     2041 gcaccagatt acttaggaaa aatggatgta ctgattgccg gtgggaagat tttggctgta
     2101 gaagaacagc tagaaggcgg ctacgaagga gtggaagtag aagaactttc aggtgaggga
     2161 aaagtactaa ccccgggttt cattgactgt catttccata ttttaggtgg aggaggagaa
     2221 ggcggttatc aaaatcgaac gccagaagtc acattgagcc aattgactac agctggtgta
     2281 acgacagttg tcggttgttt gggaacagac ggagaaggta gagatatgac tgcgttgatc
     2341 agcaaagcga aaggtctgga agcagaagga atctccactt atgtatacga aggttcgtac
     2401 cgattgccgg taaaaacagt aacagattct atcatcaaag actttttgac aattgataaa
     2461 attatcggaa tcggagaaat cgcagtatcc gatcaccgtt cttctcagcc aagttttgaa
     2521 gagttcgctc gtgcagtagc agatgctcgt gtaggaggca tgttatccgg aaaagctggg
     2581 atcatcaatg tccacttagg cggaggaaaa agaaagctag agctattgac acgaatcgta
     2641 gaagaaacag aaattccgat cacacaattt ttgcctactc atgcaaatcg cacaccagaa
     2701 ttatttgaag cttgcgtggc atttgccaaa cgtggtggca cgattgactt cactgctagc
     2761 gaagatcctg atttttggga gaagacagat ggtgaagttc gcttcagcaa agcactgaaa
     2821 cgattgatcg aggaagatgt ttctttagat aatttcacga tgacctcaga tggacaagga
     2881 agcttgcctt attttgatga aaacaatcat ttcctagggt taggagtagg aagtgccaag
     2941 gcattgctag tagggatcaa agaagctgtg caaaaagaaa gtatcccttt ggaaatcgcc
     3001 ttacgagcta taaccagtaa tccagcacgt atattgaaat tggataaaaa aggaaaaatc
     3061 gaaattggtg ctgatgcaga tctgtgtatc ttggataaag aaacgcttga tattgataca
     3121 gttatcgcta aaggagaaat aatggtccaa gaaaaagaag tcaaagtatg ggggactttc
     3181 gaaaaaagtt tttaatcaac agctctaact atctttttta gaaaacgagc gttctgtttg
     3241 tctgtatctg ttgttgatgt taccctgaag gtagatcaat aaacaaacta agaggaggaa
     3301 gcacaatggc agatttaaaa ggacgttcta aagatatgaa agataaagta gtcggtgaag
     3361 gaaaagaagc atacggcaaa gcaacagacg acaagagcaa agaaacagaa gggaaagcac
     3421 aatcactagg tgctgacatc aaagaaaaag ctcgaaattt aggcgacgaa gtcaaagaca
     3481 agttttctcg aaaagacgaa aaaaaataaa aaccacttat tactaattga atcaagcaaa
     3541 taaaaaatcc gaaccatatc tgcgagacag atatggttcg gattttttat ttgatcatta
     3601 tcctgttttt taaacttcga ttttttgaaa ttttttattt tttagttcaa agacttcatc
     3661 acagcttttt gctacttcaa tctcatgagt cacgatgata atacagcgat tttgttcatg
     3721 ggcgatttct tggaaaagtt tgacgatatc ttgtgaagtt gtttcatcta gatttccagt
     3781 aggctcatcc gctacgatta gttgatgttc acagcagata gcacgaacga tagcgactcg
     3841 ctgctgctga cctcccgaaa gtttggtcac ctgttttttt gctaatattt catcaattcc
     3901 tacacgagcc agtcgatcta aagcaacttg tcgataattc tcttgcttgg tcttagtgat
     3961 ttccattgct gctatgacgt tgtctagagc cgataaataa ggaagcaagt tgtaagcttg
     4021 gaaaataatc gagacatcat ttttacgata acttcttaac ccgattttgt tcagtgaacg
     4081 atcttgatag tatacctttc cttcttttgg tgcatctagt ccagaaataa gcgaaagaaa
     4141 agtcgttttt ccagaaccac ttgctcctaa aatagcgtac atttttccag cttcgaaatt
     4201 cagttctaca tctttataaa gcgcatcttg cggatcttga taccaaaatc ccaattgttc
     4261 tgtccgtaac atcaaattcc tcctttagtt taataggatt tttcttgggt ttaatcttaa
     4321 tatccctata gaagctaaaa agacagaaaa cagactgatt ccaaatgcta ctccggctaa
     4381 tagtcccaat tgtgcaggtt tgacattgat ttccaattcg ctgacttgat cagaaacagc
     4441 aaatggatta ttcgtggttc cttgttgacc atcgcgttga ccgccttgtt cttgtctgtt
     4501 acctggcatt tgtccaccgc caggagcttg ctggttttgt gtcgtggttt ctgtctgttg
     4561 gtcaagtagc tgctgtccca cggcattcgc gacgacgttt ccgctaagcg aagcaactcc
     4621 taatgcaaga atcatacaaa tagccacttc ggtaaacaat tggagaatga ttttacttct
     4681 ggattctcct aaagatagca agacgcctaa ctcatgccga cgctcccgaa tggtaatcat
     4741 aatgatcaga gtaagtatga taataccggc tacagccact agtaaaacaa catttttaga
     4801 aaaacttgcc acgttgttta aaggttccaa cattgactga tacatggaat catttgattg
     4861 taagctaaat gtatctgtgt cgatcagctt ctcagcttct ttgagaaact catccatttt
     4921 atctggatca gatagagtgt aaatagcaga atctatcgtg ttatcgtcag atgtcccatt
     4981 tagttcattt gcaaaagtgt aagaagtgta aagtgtattc gacggattca tgaagttgaa
     5041 tttcataccc atactgccgc ttgtttcgct ggattcataa attcctttga tcgtcatttc
     5101 ataagtggta tcttcatctt cagtactcgt gattgtgaaa gtatctccga ctgccaggtc
     5161 atttgtttct gctagagtag aatcaatgac tacattgttt gtgcctttgt cgctgtcatc
     5221 tattccttca ccatccgtga tttttgctgt tccatcagaa aaattactat tttgtgcagt
     5281 tgctgacaca ccactgattt gaaagtctgc ttgagtcatt tgcggattcc cgcccattcc
     5341 tccagcaggt tcaccgcctg catcagtagt ttctgaactg tcttcacttg aatcagaact
     5401 agtgatggca gttattccat cttttgccat agctgaggcg gaactttcaa aggaataact
     5461 ctttacatta tctaattcag ctattttttc cgcgtccgtt aaggaaactg gtgtaagaga
     5521 aaaactgcct ggatctggac gttcttcagt ggtagaaccg cttgaagaag tttgttcctg
     5581 ttttttaaat gaagcttctc gattggcact taaagtcact gttgctccaa cttctttttg
     5641 tgcttgattc gctgcttctt ctgcagcgct tcgaatcgtg aggccagcta gcacaaaaat
     5701 aagaatggct gtgaacacgg caaacaataa caatgaacgt ccccatttta cttttgtact
     5761 tttccatgca cgtttcataa aattcatatt ttgcatccct cctagtagat gcatttgata
     5821 atagacaacc tatcttaaag ctagcttaaa tcttatgctg tttgcacacc atcaacatga
     5881 ggttctgtat atataaaaag tcactatagt tattgcaaaa aatgtggtaa aatgaaaaaa
     5941 gtatgtttca aaccgacgaa gaagaaatga aagaggaata gcaaaagaaa tcctagatgc
     6001 tacagaggga tttttacgtg catagttgtt ctaacaattg ctgatactag cgatttagtt
     6061 tatttgttgt ataataatgg ggaacagctg aatacttata taagtattct ggctatagcg
     6121 tttcgcaaaa gatccatttt gttaatgaag tatatccaaa ataaaatgcc catccaactg
     6181 tcagaacgcg gatgtgctgg taattttcct gctgattctc cataagttga gaaatagtga
     6241 ttttgcatat gcttcattgg aatggctcat atatttagat aaagaagaaa atcgacatct
     6301 ttaaacttgg atgtcataag aagaggtgaa gtttgtgagt accattaaaa ttatcccatt
     6361 aggcggcgtt cgtgaaaatg gaaaaaacat gtatgtcgca gaagtgggcg aggatatttt
     6421 tattttagat tgtgggctaa aatatcctga aaatgagttg ttggggatcg acgtcgtaat
     6481 ccctgatttt acgtatttag aagaaaacat tgatcgtgta gctggtgtct ttttaacaca
     6541 tggacatgca gatgcgatcg gggcacttcc ttacttgttg tcaaaaattt ctgtaccagt
     6601 attcgggaca gagttgacga tcgagttagc aaaattgaat gtcagtcgta atgaagcagc
     6661 caaaggattt aaggatttcc atgtggtaga tgagcataca gaaatagact ttggtgaaac
     6721 agtagtgagc tttttccgta ccactcatac gataccagat tcagtgggga tcaatctgaa
     6781 aacaaaagaa ggtagtatcg tttatacggg agattttaaa tttgaccaaa cggctatccc
     6841 aatgtatcaa acagattatg cacgattggc ggaaatcggc aaagaaggag tcttagcctt
     6901 actaagtgaa tcggcaaatg cagaaaatcc tcagcctgta gcttctgaag cccaaatagc
     6961 agatgaagta tttgatacga tcaaatattg ggaagggcgt atcatcgtcg cttgtgtagc
     7021 aagtaatttg caacgagtgc aacaagtatt ggatgcagca gaaaaagcac aacgaaaagt
     7081 tgtcttgact ggtcaagatt tcgaacgaat tattcgcaca gcgatgcgtt tagaaaaact
     7141 tcaattacca agtgaagatc tgcttgttac gttgaaagac atgaaaaact atgatccaga
     7201 agaactgatc attttagaaa ctggccgaat gggtgaaccc atcaagtctc tgcaaaaaat
     7261 ggcaaacggt acacaccgta atgtcaagat tgaagaagga gatttggttt atatcactac
     7321 tacgccaagc atcgcgatgg aaaccatcgt ggcgaaaacg gaagatatca tttatcgagc
     7381 tggtggaaca gtgaagttga tctctgagaa tatgcgcgtt tctggccatg ccaatcctaa
     7441 tgacttgcaa ttaatgatca atttgatgaa accaacatat tttgtgccag ttcaaggaga
     7501 gtatcgtgaa ttagctgctc atgcagattt ggctcatgca gtagggatgc catacaaaaa
     7561 tatctatatt actggacgcg gagacgtgct ggaatataag aacaagcgta tgagtgttgc
     7621 aggaactaca agtgcagaaa atgtaatgat tgatggatta ggcataggtg atatcggaaa
     7681 cattgtttta cgtgatcgaa aaatcttgtc agaagacggg attttcgtag cggttgtcac
     7741 tatcagtcgg cgagaaaaga aaatcatttc aaaacctcaa attacttcac ggggattcgt
     7801 ttacgtaaaa gctagccgtg atctgatgaa agaaagcagc aatatgattg aaacaatcgt
     7861 tgagaaacat ttagaaagca atgattttga gtggagcaaa ttaaagcaag acatccgaga
     7921 tcaattaggc cgttacttat ttgaacaaac aaaacgccgc ccagtgatct tgccagtcat
     7981 tatggaagct actcaacgtc gaaataaaaa ataaacaaaa agagtaagat gaacctctct
     8041 atagagtgct cgtcttgctc tttttttgcg aaaaacaaat aatttttgaa tacagcatcc
     8101 ctcgctttat aataaacgat agaataaagg gagtgaatca atgatgagtc aaacaaaagt
     8161 cgaacgttat ttagaagaac aaaagatcag ctatcaacca cttgatttta gtcatgtggt
     8221 agaagaatct ttcacagaag aactacaaaa aagagggatt gatccagctt tgatatgtaa
     8281 aactctcgta gtgaaaggaa acaaaactgg ggtgatcatc gcattgattc ctttgaacga
     8341 tcacttagat tacaaaaaaa cgagacaagt gacgaaagat cgtaaagttg gttttcctgg
     8401 aatggatttt gtattaatgc atacaggata tccccacgga gcaaacaccc ctattggaat
     8461 aaaactcgca catcctgatt attgtttttt agcagacagt tctttaaaag aaaaagaaga
     8521 agtaattgtg tcaagcggtg aaatcgggag aagccttcgg ataaaagtag ccgatttaga
     8581 aaagatcgtc catcctattt acgctgattt aattagttaa gggacgagaa gagaagctaa
     8641 gcagagcaga gacctcaaac ttgagattat atctgcttgg tacttggtgc atcctgtatt
     8701 atctgttaca attgctttgt acatactgta ctatcaatcc aatgaaaata tatttagcag
     8761 ctatattttt tcattgatgt gcaagtgatg aaatcgtttt taataaaaaa gatacagaga
     8821 aaacgcgagg tgtaaaatat gaaaattacg tatcatggac attcctgttt aacaattgaa
     8881 atggaaaatg gccaacgcgt gatgattgac ccgtttatta caggaaatcc attgacagac
     8941 ttaactgcag aagaagtaaa gacagattgg ttgctgatta ctcatggtca tagtgaccac
     9001 ataggagata tgattcctat tgcaaaagaa aatcaagcca caattatcag tattgttgag
     9061 attgctgatt atgcgaacag cagaggagta gattcattcg gtatgaatat cggtggaaaa
     9121 catgcgtttc cattcggcgt ggtaaaattc gtacatgcac agcatagttc aagctatgaa
     9181 gtagacggta tcgttcagta tatgggcgag cctagcggaa ttattattca agcagaagga
     9241 aaaacgattt atcatgcagg ggatactgct tatttcagtg atctaggatt gcttgcagaa
     9301 gaattcgata ttgatgtcgc tttcttgccg attggcgata actatacgat ggggccagaa
     9361 gatgcagcaa aggctgcgca aacaattcgt gccaagaaag tagttccaat ccattacaat
     9421 actttccctg tcattgaaca agatcccgaa tcttttgtag agttactgga agatagtgaa
     9481 ggaaaaataa tgagcgtctc ggaaacgctg gaagtgtagg cagaaaatgg cgacaaaaca
     9541 tgatttgatt ttagaatata ttgaaagttt acctgttggc aatcgaattt ctgtaagaag
     9601 catcgcaaaa gaattgaatg tcagtgaagg aacagcgtac cgtgcaatca aagatgcaga
     9661 aaatgtcgga ctagtttcaa caattcaacg agtagggacc atccgtatcg aacgcaaatt
     9721 aaaaaaacat attgaacgtt tgacatttgg cgaggtcgtc aggatcattg aaggggacgt
     9781 tttaggcggg gcggcaggat tagacaaggt cttgaataaa ttcgtcatcg gcgcgatgac
     9841 taaacatgcc atggaacgct atattacgcc aggatcacta atgatcgtcg gcaaccgggc
     9901 ggaagtccaa aaattagcat tggaagatgg cgccgcagta ttgatcacag gaggattcaa
     9961 tacgactgct gagattgcaa agctagctga tgatcttgaa atgccgatct tacgcacctc
    10021 ttatgataca tttacagtcg caaccatgat caaccgagcg ttaagcgacc aattgatcaa
    10081 aaaagatatc atgcttgtca gtgatatcta tatgccagct gaaaagactc attatttgca
    10141 gcagacagat acgatttctg attatcagcg tttgtcagaa gaaacgcagc attcgcgttt
    10201 tccagtcgtc aaccgccatc atcgtttgat ggggattgtt acggcaaaag atgttttagg
    10261 gaaatcgcca aaccagatca tcgatcgggt gatgacgaaa gaaccaatca gtgtaaagaa
    10321 aacaatgagt attgcctctg ttagccatca aatgatctgg gatgggctag aagtcatgcc
    10381 ggtcgtttcc gatgacttga cattggaagg cttgattacc cggcaagatg taatgaaagc
    10441 gatgcagctt gtccagcgac aaccgcagat tgcagatacg atttcggatc agatatctgg
    10501 aaatatccat acgattgata ctgaccgtga aggaaatcgt ttggaaaacc ctaagttcaa
    10561 attcgtagta gcaccacaaa tggtcaatgg cgttggaacc atttcttttg gcgtacttag
    10621 cgaaatcatt tctgatgtcg cacaaaaaac aatggtgatg aaccaaaaac gaaatatctt
    10681 gattgaacaa gtgaacctac attatttgcg gctgatccaa ttggaaagcg aattagacat
    10741 tcgtccacgt gtattggaaa tcggcagacg ttccgctaaa ctggatatcg aagtgtttat
    10801 tgaaaacacc atcgtttcca aagcaatcgt tgtttgtcaa gtaatggaac gttcttagaa
    10861 agcaggagtg aaatgacagt acagcaagaa atagtagata caataaaaaa atacgataaa
    10921 atcattatcc acaggcattt gcgccctgat cctgatgcat taggatcaca agtaggatta
    10981 gccgaactat tacgggaaag tttcccagaa aaagaaatac gtcaagtagg tgaaacgatt
    11041 gaagggcttc gctttttagc agaaatgcaa gaagttgaag atgtcttcta ccaaggagcg
    11101 ctagtcatcg tgactgatac agctaattca cccagaatca gtgatcaaag atatcagttg
    11161 gcagaaaaac tgatcaaaat cgaccatcat cccaatgatg acccttatgg agatcttgtg
    11221 tgggtaaata caaaagcaag tagctgtagt gagatgattg cagaatttgc tttgatgttt
    11281 cctgaatctt taaagatgaa cgttaaggct gcacggttac tttatgcagg aatcgtcgga
    11341 gatacagggc gctttttgta tccctccacg acgtcccgta cattagagat tgcttcgata
    11401 ttgagaaatt atccatttga tgcaagtgct ttgaacagag agattgaaca aatgccgatg
    11461 aaagtcgcta aactttccgg ttatctttat caaaatatcc aggtggatga aaatggtgca
    11521 ggaagaataa tccttccgca agaaattctt gataaatatg gaatcgatga ttcagagaca
    11581 gcttcgatcg tttcacttcc tggagtgatt gacgaagtgc tggcttgggg gatttttgtt
    11641 cagcagcctt cagggtatta ccgcgtacgt ttacgttcta aggggccgat catcaatgaa
    11701 ttggctaaac gccatcatgg aggaggccat ccattagcaa gtggtgcgaa tgcaaaagat
    11761 ttagcagaag tagaggaaat ctataaagag atccaagata tatgccaatc gtataagagc
    11821 agaaaaacag aatgatactt cgttcgaaaa cgtgcctttt gtagtaaaaa tcacaaatgt
    11881 atcttttcaa gatcttcttt ttccgttaca atagtgctca ctaaagattt taaaggagaa
    11941 agaagatgac attaccaaat tgcccagaat gtggctcaga gtacacatat gaagatcgag
    12001 gcttgttgat ttgcccagaa tgcgggaacg aatggaacaa agaagaacaa gaaactgtca
    12061 tagaagacgg attagtggta ttagatgcaa atggaaaccg tctgttagag ggcgatagcg
    12121 tgacagtgat taaagacttg aaagtaaaag gtgcgtcagg tgccattaaa caaggaacaa
    12181 aagtcaagaa catccgttta gtagaaggcg atcataatat cgattgcaag gtagaaggat
    12241 ttggaccgat gaaactgaaa tcggaatttg tgaaaaagaa ttaaagaata ttcaaggtct
    12301 tttcacattc ttttcctact actaatgata aaataaagta aacgaaaagg agtgaaacga
    12361 aaatgtatgg ttatggaatg tatggttttt tagatccgac atttcttttg gtcattcttg
    12421 gattagttat ttccggaatt gcgtcagcgt acgtcaatag tactttccga aaatacgatc
    12481 aagtgagaag caaaaacaat gtgactggta cacaagcagc acaatatata ttacaaagtc
    12541 aacagataaa taatgtcggt gttcaacaaa ttgccggaga tctcacagat aactacaatt
    12601 caggtaataa gatgttgagt ttgtctcaag caactgcaca gtctacttca gttgctgcga
    12661 taggtgtagc agctcatgaa tgcggacatg cggtacaaga tgcttcgggt tatgtcccat
    12721 tgaagattcg tgcagcactt gttccagcag ctaactttgg ttcgatgatt tcgttcccct
    12781 tgattatggt aggtgtattg cttagctgga actctacgtt aatcaatatc gggattttcg
    12841 ctttttcttt agcattactt ttccaattgg tgacattacc agtcgaattt aacgcgtctc
    12901 ggcgagcaat ccaaattttg agtgaaggcg gactactgac ggaagaagaa gtaccgatgg
    12961 caagacacgt attatttgca gcagcactga cttatgttgc agcagcgctt tcgacattcc
    13021 tgcagctgtt gcgcttgatc ttattgttcg gcggcaaccg aagagattaa aataaagaag
    13081 ttgtgaaatc agcgctttga tctgagctaa taccgagagt tagctgattg aataccattt
    13141 atttggaata agggctcttt gtcaataagg actgatgggt tgtacaaaat caaatctggg
    13201 tcagaatgaa cctcattctg gctcag
//
LOCUS       DARBFO010000071        12838 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000071 DARBFO010000000
VERSION     DARBFO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.072"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            6..305
                     /locus_tag="SCK51_002132"
     CDS             6..305
                     /locus_tag="SCK51_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1252746.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="SCK51_002133"
     CDS             378..1739
                     /locus_tag="SCK51_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEG1252747.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="SCK51_002134"
     CDS             1761..2021
                     /locus_tag="SCK51_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252748.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="SCK51_002135"
     CDS             2035..2787
                     /locus_tag="SCK51_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEG1252749.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="SCK51_002136"
     CDS             complement(2881..3486)
                     /locus_tag="SCK51_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1252750.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="SCK51_002137"
     CDS             complement(3539..4513)
                     /locus_tag="SCK51_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEG1252751.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="SCK51_002138"
     CDS             complement(4891..5610)
                     /locus_tag="SCK51_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1252752.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="SCK51_002139"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="SCK51_002139"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEG1252753.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="SCK51_002140"
     CDS             7161..8558
                     /locus_tag="SCK51_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1252754.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="SCK51_002141"
     CDS             8639..9079
                     /locus_tag="SCK51_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1252755.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="SCK51_002142"
     CDS             9227..9553
                     /locus_tag="SCK51_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1252756.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="SCK51_002143"
     CDS             9554..10303
                     /locus_tag="SCK51_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252757.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="SCK51_002144"
     CDS             complement(10383..10838)
                     /locus_tag="SCK51_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEG1252758.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="SCK51_002145"
     CDS             complement(11265..11540)
                     /locus_tag="SCK51_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252759.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="SCK51_002146"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="SCK51_002146"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEG1252760.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4109 a         2211 c         2621 g         3897 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagctt
//
LOCUS       DARBFO010000072        12784 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000072 DARBFO010000000
VERSION     DARBFO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.073"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="SCK51_002147"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="SCK51_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEG1252761.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="SCK51_002148"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="SCK51_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEG1252762.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="SCK51_002149"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="SCK51_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEG1252763.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="SCK51_002150"
     CDS             1642..2274
                     /locus_tag="SCK51_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEG1252764.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="SCK51_002151"
     CDS             complement(2276..3181)
                     /locus_tag="SCK51_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1252765.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="SCK51_002152"
     CDS             complement(3266..3925)
                     /locus_tag="SCK51_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG1252766.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="SCK51_002153"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="SCK51_002153"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEG1252767.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="SCK51_002154"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="SCK51_002154"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEG1252768.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="SCK51_002155"
     CDS             6168..7253
                     /locus_tag="SCK51_002155"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEG1252769.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="SCK51_002156"
     CDS             7250..8314
                     /locus_tag="SCK51_002156"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEG1252770.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="SCK51_002157"
     CDS             complement(8563..9366)
                     /locus_tag="SCK51_002157"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEG1252771.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="SCK51_002158"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="SCK51_002158"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEG1252772.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="SCK51_002159"
     CDS             complement(10244..11251)
                     /locus_tag="SCK51_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEG1252773.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="SCK51_002160"
     CDS             complement(11258..11902)
                     /locus_tag="SCK51_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEG1252774.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="SCK51_002161"
     CDS             complement(12002..12760)
                     /locus_tag="SCK51_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEG1252775.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
BASE COUNT         3620 a         2706 c         2441 g         4017 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tata
//
LOCUS       DARBFO010000073        12734 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000073 DARBFO010000000
VERSION     DARBFO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.074"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="SCK51_002162"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="SCK51_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEG1252776.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="SCK51_002163"
     CDS             complement(322..891)
                     /locus_tag="SCK51_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEG1252777.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="SCK51_002164"
     CDS             1077..1979
                     /locus_tag="SCK51_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1252778.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="SCK51_002165"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="SCK51_002165"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEG1252779.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="SCK51_002166"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="SCK51_002166"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEG1252780.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="SCK51_002167"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="SCK51_002167"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEG1252781.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="SCK51_002168"
     CDS             8547..8732
                     /locus_tag="SCK51_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEG1252782.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="SCK51_002169"
     CDS             complement(8794..9990)
                     /locus_tag="SCK51_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG1252783.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="SCK51_002170"
     CDS             complement(10160..11596)
                     /locus_tag="SCK51_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEG1252784.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="SCK51_002171"
     CDS             11889..12698
                     /locus_tag="SCK51_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1252785.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DARBFO010000074        12715 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000074 DARBFO010000000
VERSION     DARBFO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.075"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            3..935
                     /locus_tag="SCK51_002172"
     CDS             3..935
                     /locus_tag="SCK51_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEG1252786.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1007..2128
                     /locus_tag="SCK51_002173"
     CDS             1007..2128
                     /locus_tag="SCK51_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1252787.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(2210..3982)
                     /locus_tag="SCK51_002174"
     CDS             complement(2210..3982)
                     /locus_tag="SCK51_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1252788.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(3985..5697)
                     /locus_tag="SCK51_002175"
     CDS             complement(3985..5697)
                     /locus_tag="SCK51_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1252789.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            5812..6480
                     /locus_tag="SCK51_002176"
     CDS             5812..6480
                     /locus_tag="SCK51_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEG1252790.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(6547..7335)
                     /locus_tag="SCK51_002177"
     CDS             complement(6547..7335)
                     /locus_tag="SCK51_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEG1252791.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(7418..8890)
                     /locus_tag="SCK51_002178"
     CDS             complement(7418..8890)
                     /locus_tag="SCK51_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1252792.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(9020..10213)
                     /locus_tag="SCK51_002179"
     CDS             complement(9020..10213)
                     /locus_tag="SCK51_002179"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEG1252793.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     regulatory      complement(10211..10290)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            complement(10479..10550)
                     /locus_tag="SCK51_002180"
     tRNA            complement(10479..10550)
                     /locus_tag="SCK51_002180"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10515..10517),aa:Glu,seq:ttc)
     gene            complement(10562..10635)
                     /locus_tag="SCK51_002181"
     tRNA            complement(10562..10635)
                     /locus_tag="SCK51_002181"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10599..10601),aa:Ile,seq:gat)
     gene            complement(10648..10718)
                     /locus_tag="SCK51_002182"
     tRNA            complement(10648..10718)
                     /locus_tag="SCK51_002182"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10684..10686),aa:Gly,seq:tcc)
     gene            complement(10723..10795)
                     /locus_tag="SCK51_002183"
     tRNA            complement(10723..10795)
                     /locus_tag="SCK51_002183"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10760..10762),aa:Phe,seq:gaa)
     gene            complement(10799..10871)
                     /locus_tag="SCK51_002184"
     tRNA            complement(10799..10871)
                     /locus_tag="SCK51_002184"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10836..10838),aa:Asp,seq:gtc)
     gene            complement(10890..10963)
                     /locus_tag="SCK51_002185"
     tRNA            complement(10890..10963)
                     /locus_tag="SCK51_002185"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10927..10929),aa:Met,seq:cat)
     gene            complement(10976..11065)
                     /locus_tag="SCK51_002186"
     tRNA            complement(10976..11065)
                     /locus_tag="SCK51_002186"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11027..11029),aa:Ser,seq:tga)
     gene            complement(11088..11159)
                     /locus_tag="SCK51_002187"
     tRNA            complement(11088..11159)
                     /locus_tag="SCK51_002187"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11124..11126),aa:Glu,seq:ttc)
     gene            complement(11181..11268)
                     /locus_tag="SCK51_002188"
     tRNA            complement(11181..11268)
                     /locus_tag="SCK51_002188"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11232..11234),aa:Ser,seq:gct)
     gene            complement(11293..11366)
                     /locus_tag="SCK51_002189"
     tRNA            complement(11293..11366)
                     /locus_tag="SCK51_002189"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11330..11332),aa:Ile,seq:gat)
     gene            complement(11380..11450)
                     /locus_tag="SCK51_002190"
     tRNA            complement(11380..11450)
                     /locus_tag="SCK51_002190"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11416..11418),aa:Gly,seq:tcc)
     gene            complement(11452..11527)
                     /locus_tag="SCK51_002191"
     tRNA            complement(11452..11527)
                     /locus_tag="SCK51_002191"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11492..11494),aa:Asp,seq:gtc)
     gene            complement(11546..11619)
                     /locus_tag="SCK51_002192"
     tRNA            complement(11546..11619)
                     /locus_tag="SCK51_002192"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11583..11585),aa:Met,seq:cat)
     gene            complement(11632..11721)
                     /locus_tag="SCK51_002193"
     tRNA            complement(11632..11721)
                     /locus_tag="SCK51_002193"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11683..11685),aa:Ser,seq:tga)
     gene            complement(11745..11818)
                     /locus_tag="SCK51_002194"
     tRNA            complement(11745..11818)
                     /locus_tag="SCK51_002194"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11782..11784),aa:Ile,seq:cat)
     gene            complement(11832..11905)
                     /locus_tag="SCK51_002195"
     tRNA            complement(11832..11905)
                     /locus_tag="SCK51_002195"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11869..11871),aa:Met,seq:cat)
     gene            complement(11933..12005)
                     /locus_tag="SCK51_002196"
     tRNA            complement(11933..12005)
                     /locus_tag="SCK51_002196"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11970..11972),aa:Ala,seq:tgc)
     gene            complement(12025..12098)
                     /locus_tag="SCK51_002197"
     tRNA            complement(12025..12098)
                     /locus_tag="SCK51_002197"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12062..12064),aa:Pro,seq:tgg)
     gene            complement(12108..12181)
                     /locus_tag="SCK51_002198"
     tRNA            complement(12108..12181)
                     /locus_tag="SCK51_002198"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12145..12147),aa:Arg,seq:acg)
     gene            complement(12192..12277)
                     /locus_tag="SCK51_002199"
     tRNA            complement(12192..12277)
                     /locus_tag="SCK51_002199"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12241..12243),aa:Leu,seq:taa)
     gene            complement(12294..12365)
                     /locus_tag="SCK51_002200"
     tRNA            complement(12294..12365)
                     /locus_tag="SCK51_002200"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12331..12333),aa:Gly,seq:gcc)
     gene            complement(12377..12449)
                     /locus_tag="SCK51_002201"
     tRNA            complement(12377..12449)
                     /locus_tag="SCK51_002201"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12414..12416),aa:Thr,seq:tgt)
     gene            complement(12467..12548)
                     /locus_tag="SCK51_002202"
     tRNA            complement(12467..12548)
                     /locus_tag="SCK51_002202"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12512..12514),aa:Leu,seq:tag)
     gene            complement(12556..12628)
                     /locus_tag="SCK51_002203"
     tRNA            complement(12556..12628)
                     /locus_tag="SCK51_002203"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12593..12595),aa:Lys,seq:ttt)
     gene            complement(12632..12704)
                     /locus_tag="SCK51_002204"
     tRNA            complement(12632..12704)
                     /locus_tag="SCK51_002204"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12669..12671),aa:Val,seq:tac)
BASE COUNT         4089 a         2717 c         2245 g         3664 t
ORIGIN      
        1 ctttgttaaa attaaaagaa aaggaatata ttattgacga tgctgtttct gtccctctta
       61 ttttaaataa acgagagaaa aataatttaa agaaaaaaat gctcattcca tttgattcag
      121 aagtgaaagg attactcaat aaactaacta tttctaatat agactcgact agggaaaaaa
      181 tcataaaaat atttgataaa attaataatt tattcaagaa tgacaatgct gaattaagga
      241 agggtctttt agagtttaaa cttcaagaac tagaacttca ttactcttat ttagtgaaaa
      301 ttaccgaaga aaaagaacaa caaaaagcaa taaaagaaca gatgatagaa gaggaaaaag
      361 ttcgtagaga aattgatcgt gaaaagaaac gtattgataa agaacaacgt caattcaatt
      421 ctgaaatttc caaattaatc gcatatatgc aaaaagctaa tgcagatgtg gaaaaagaat
      481 tatatgcaaa taaaattcaa gaactggaag aaaaattaaa agaattagaa gttatacgtg
      541 aaaatgtatt acaaagagaa ttaaatacta gagccggtta tgtgtatgta attagtaata
      601 ttgggtcttt tggtgaagac atatataaaa ttggtatgac tagaagatta gaacctatgg
      661 atagaataaa agaattgagt agtgcatcgg ttccatttga atttgacgtt catgcgatga
      721 ttttttcaga ggacgctccg tctttggaaa ctaaattgca taatcatttt agaaaacaag
      781 aagttaataa aattaatcaa agaaaagaat ttttcaaagt ttcactagac gaaatagaga
      841 aagttgtttt agaaaattat aatggaacag taacatttac aaaattagct aaagctgaac
      901 aatatagacg atctttagaa ttgtcaaaag attaaatata taaaaagcct tcgggctttt
      961 tcttttaaca attaatagga acgtatattc gaaaggaata gaaataatgt ggttagaaaa
     1021 aataaatgac aaaaaattta agtacaccga acgttatacg gatcccttaa ctgaaaaaaa
     1081 gagaaaagtg tccgttactt tatctagcaa ttcacgccaa gcttggaatc aagctaactt
     1141 acttttgaat gaaaaaatcg cagaaaaaat aaaacgaaat gaagaattac cactaacttt
     1201 tggagaatta aaaactaaat gggatgaaaa atacaagcca actgtaaaag aaagttccta
     1261 tcgaacaact caggtttatc tatcattaat ttcaaaatat ataaaagatg atgtacttgt
     1321 aaaaaacgtt aatagtaatc ttatacaaga tatgctagat tactactatt ttgaaaaaaa
     1381 tttatcatat aattatgtaa tacatttaaa aacttttaca ggaatgattt ttaaattcgc
     1441 caaccgtcga tatggattaa aatataatcc cgtaaatggg gtttctttag caagaaaacc
     1501 taagacaaac gaagaaataa aaaaagaaaa agatggttat ttatcaaaag aagaagttca
     1561 aatgattgct agtaaacaaa aaagttcaca tcaacagtca cgttattctt taattactaa
     1621 atttttgttt ttaactggac tacgttatgg tgaattgata tctttgactg aatctgatta
     1681 tgatggtaat aaaattacgg tttctggtac ttatgattac gagttaaaaa ttaaaacaac
     1741 aactaaaaat acaggttctt atagaaccat tgaattatca agtaatgcta aagaaatcat
     1801 agatcaatta attgaagaaa acaaattaat taagaataat aaagataaat acattttcat
     1861 ctctaaaaat ggtaacccga tatctattca agcatataat caatctttaa gagcagtatc
     1921 caaagaatta aacctagata aaaaagtttc ttctcacatg ttaaggcatt cccacatttc
     1981 tctacttaca gaattaggaa tcccattaaa agccattatg gatcgtgtag gacatgaaga
     2041 ttcaaaaaca acattaaaga tttatactca tacaactaaa aatatgcaaa atcaactagt
     2101 agaaaaacta ggaaaaatag aaatttaaat tgcccctttt ctgcccctta taaaaacaaa
     2161 caacataaaa tatataaaaa gaaccccgtt ataacgaggt tcttttaatt taactttcaa
     2221 atacaaattg attatgataa agttccgcat aaaatccatt ttctgctaat aactcgtgat
     2281 gactgccttc ttcaatgact tccccatcac gcaagacaac gattctatct gcattcaaga
     2341 ttgttttcag ccggtgagca atcacaaagc tggttcttcc tttgattgct tcatccattg
     2401 ctttttggat ccttgcttct gttaccgtgt ctacattact tgttgcttca tccaaaatca
     2461 ataaagatgg atttgtgatg atcgttcgag caatactgat caactgcttt tgacctgtac
     2521 tgaataagtt attttcttcg gatatctctg tatcataacc tttttctaac gtcatgatga
     2581 agtcatgaat attcgcttgt tttgctgcat tgatgacttc ttcatctgaa gcgtccggtt
     2641 tgccgaatgt gatattgtct ttgatcgttc cggagaacaa tacagattct tgcaaaacga
     2701 ttcctacatg tctacgtaat gcatcaagat cataatctcg gatatctatt ccatcgatga
     2761 ctactgaacc atcattgaca tcgtaaaaac ggtttagcag attcatgatc gtcgttttcc
     2821 ccgatcctgt tggaccaaca agagcaacca tttctccttt gtccacattg atcgagacat
     2881 ctttcaagac tagtttttct ggagaataac caaaatctac gtgctgcaac gcaagacctt
     2941 tttcaacacc atcaaatgct ttacagtttt ctggtttgac ttcatctttt tcatcaaata
     3001 tttcattcaa tcgtcttgcc cctgtaatcg ctaattggat catgctgtat ccagatgaaa
     3061 tctgcatcag tggttggtaa tattgttgtg aatactggac gaatgtcaca actaagccta
     3121 atgccaccga acgttccaaa tcaccattta atgctaacca gccgccgaaa aagataacta
     3181 tcgctgtatt gaccagtgac attccttgca tcatcggaaa tagtagacca gaataagctt
     3241 gtcctttgaa ggttgcttcg cgaacacttt gattatgctc taagaatcct tcaatcgttt
     3301 cttcttgtaa gccatttgtg atgatgacac gttgtccgct gattttttca tccatataac
     3361 catttagttt ccccacttca tcttgctgaa gatccacata tttccgtgct ttgcggataa
     3421 tcaagacagc tatcaaaacg gctactggag tggaagcaat tgtagcccaa gctaactcta
     3481 cattttggcg gaacatcatg atcaagatcc cgacaagtaa aacagcattc gtgatgactt
     3541 gaagcaaagc ttggttcaaa ctattttgaa tattatctaa gtcactggta aaacgactta
     3601 aaatttcacc gtcttgatga gaatcaaaga aacggatcgt caatttttcc aatttgttga
     3661 atagaccgat acgcattcgg ttcgttgact tccctactac ttgtgtgaac aaaatactat
     3721 agataaagtt ggctgcacta gctagtacat aaaacagcaa gagtttccat attacagaaa
     3781 caaatttgct tttatcgtct acgccttgca tcaagcctcc tacgtaatta gctaattctt
     3841 gaaaagcttc cccgatgtat tgaggtgcct ttacttgtag ataggtagca atgatcactg
     3901 tgataaagat aaagaaaaag gagagtttgt accgcttcaa atagtgatag aaaaatttac
     3961 ttgcttttat tagatcagtc atacttactc ctccgttcct ttttgagttt caaagatttc
     4021 ctgatagaca cggtttccag ctaccaattc ctcatgggtt ccttcgccga ctaatctgcc
     4081 ttcatccaat actaagatgc ggtctgcttt gacaacagaa gcaatttttt gtgcgatgac
     4141 aatcgtcgtt gttcccttca aatctcgatc gagtgcttct cggaccagct tttcagagcg
     4201 agcatccagt gcactcgtac tgtcatctag gatcaagatc tttggattac cgataacgcc
     4261 acgagtaatg gataaacgtt gcttttgacc accagaaaag ttactgctac gttcttctat
     4321 tggtgcttcg taacgatcag ataacttttc gatgaattct tttgcttgag caatagaaga
     4381 agcatgttcc atctcttttt ctgttgcatt ctttttccct tggcgcaagt tttgagcaat
     4441 cgttcctgag aataaaattg ctttttgcaa aacaaaggaa accgtttgtc ttaaactctt
     4501 ttcatttact tgacgaagat ctacaccacc tacttcaact ttgccttctg tcggatcaaa
     4561 tagacgaggg atcaattgtg ctagtgttga cttcccggca ccagtcgccc cgacgatacc
     4621 aaccatttct ccaggtttga tagaaaagct gatatcttta agcgtatctg tatcctcacc
     4681 aggataacga aaagaaacat ggtcaaaagt gacagaaccc gtcaactctt gttctggaac
     4741 gtctaaataa gtaagatctg gttcagcatc taaaatttcc gcaatccgct tcagcgatac
     4801 ggctgctctt gaagtcatca tcatcatcat tccgccaata atgattgcca tcataatctg
     4861 catcaagtag ttcatgaatg aagcaattcc accaattaac gtaggatcat ctttaacaag
     4921 atcgctcaca aaaaagatcg ccccaaccac agccaaattg gcagccagca tgaaggacgg
     4981 gatcatcaca gaaaataatg taccgactat gatgttgtgt tttgttaagt cctcacttac
     5041 tttgctgaat gacttcaatt ggttttcttc ttgtacaaaa gatttcacta cacgaatccc
     5101 tagtaagttt tcttttgcaa tgctattcac acgatcaatc agtttttgaa tgatcatgaa
     5161 gtgcttgccc atccttgtaa atgaaagtgc cgtaatcaga aataccgtaa cgataagcgc
     5221 tacgatgatc caccataatt gcggcatcgt gatcatagct aaaatgaagg caccaataaa
     5281 taagaatgga attcgaaaaa gtgattgcaa agaaatcatc actaaatttt ggatttgcgt
     5341 gatatcattt gttagacgaa caacaagatt tccagcagag aactgttcga tatttccaaa
     5401 tgaaaatgtc tggatctttc gaaatgcttc ttctcggata tctgcactta cgccttgagc
     5461 tacttttgca gagaaaatcg tgttcgttac acctgcggct aatcctagca aagccagact
     5521 gatcaggtaa ataccaattg ttttcatttc atcattatct tctgtaataa tagcttccaa
     5581 tacttgctgt agtaacttag gttgccataa tgctgctgca accatgactg ctacggacag
     5641 caaggaaata aaaacagcta ctttatattt tttcgcatgt cgtaagatta atttcattcg
     5701 ttgattacac ccctttgttt aaattgacag gactaattta acactgtaaa attagaccga
     5761 ctaacaattt accatgttta aaaagataat gcaatacacg aggttgataa aatggaaata
     5821 aagctttctc aagcaatcat cattcaggcc gcctttgata tcctggctga aacacaagct
     5881 ctttcgcagc tttccatgcg aaacatcgca aacagaattc atgttcaagc acctgcttta
     5941 tattggtatt tcaaaaacaa acaacagctc ttgcaaataa tggctgaaac aatggaagaa
     6001 gaattagcgc ttccagatcc aactttgcct tggaatgaac agttgctgca gtatatggaa
     6061 aattactatg acctttatac acgtttccct tgtggggcag aattagaaat caatactgtg
     6121 cccgcttttc cgagtcgatt agaacatttg gagcaaatga tccaattact tgttcgtgaa
     6181 ggattttcag tcacccaaag tcatcaggcc attgcctctc tccacaattt attgattgga
     6241 cagttaatgg atcagcagca tgaacagcag cttcgacaaa aaattatgga cggaaatgaa
     6301 tttttgaaag aaacagttct atttatgcgt aattatgtag ttgaaaatca attagaggga
     6361 tttaaagaaa gtatgcagtc ccatgcagaa ttgaatgaaa aagcactctt tctaacgagt
     6421 gcacaaacct atattgaagg cttatgccaa caaaataaaa gaagagaaaa cgagcagtaa
     6481 aaaagctcct atagagatca ctatgtttgt gatctctata ggagctatct ttttaagact
     6541 ctttttttat ttttcttcca ttagccacgc caatgcacgt tgaatccatt ggtcccagaa
     6601 gatccagtta tgatcgccag gtccttgttc aaaggtgacc gccaagcctt gttcctgcat
     6661 agcagatacc atttcctgat ttgcctgata aagaaaatca gattccccac aagccatata
     6721 aaatcttgga actggttttc cgctttttac tagttctttc aataagtatt gcggatcatt
     6781 ttttgaacct tcgatttgat ccaatggtcc aaagatccct tcccaaaaag atgctttacg
     6841 aaccatcaaa agatcatcca ttcgatttcc tacagaaaca gcgcctgaaa gagagattac
     6901 tgctgcgaaa ttttctgatt ttgcaaggcc tagttttaac gcgccatatc cgcccatcga
     6961 taatccagcg gcatatgttt tttctctttt tcttgttaat tgcgggaata attcatgaca
     7021 aatctcaggt agctcttctg caatgaatgt ccaatacttc atgtcataaa ctgtatctgt
     7081 gtaccagcca agatcagtag atggcatgat gacagctaat ccgtagtttg ctacataacg
     7141 ttcaatcgac gtacgtcttt cccatacact atgattgcca cccattccat gaagtaaata
     7201 taaaacaggt atatccgttg tctcttgctc tgaactggtg ccaatcacct tttttgttgt
     7261 ttgcggtagt atcacattta gattgacttc catttctaac acattcgaat aaatatttgc
     7321 ttgaataaat gccatatttt cgccttcctt agtaagaagt tatttttgaa taactttaaa
     7381 tttgaatgat tcggctacgt acatatctta accgaaacta ctgttttatt ttaccacttt
     7441 tttcatgaac gaaaagcgtt aagaccaaag caatgatcac gagtaacaag ccaactagat
     7501 aaacggaatg taacccatca taaagaattt ttcttaatgg taacaaaata tccgcatcaa
     7561 tatatttagc cgtttgtgga ttcaccagtt tattcatgat atctgcatca tttgttaaat
     7621 ttgctgctgc aagttctcgt ttagttaccc catttatcaa taaaccaaaa atagaaacca
     7681 tgactgtttg accaattgtt cgaactaacg tattaaaaga agttgctaca ccaagctggt
     7741 cacgagatac actattttgt gcactgacag tcgaggtagt catggtcaca cctaagccta
     7801 taccgatgac cgttgatata gcaaaaaacc ataaatagtt acttctgaaa ggcataagat
     7861 acaaccaagc gcccccaata gctgcgatta gtagtccaat tctcaagacc caatgagttt
     7921 tcatttgagt catccacttg cttgcaagga aagaaccgac catccagact agtgataatg
     7981 gtgctaatac cagcccacca attgctgcat tcagccctaa tacaccttgc atccacatag
     8041 ggatataaac atcgattccc atcaaaaaac cactagccaa tgctgcagcc aagttgacca
     8101 caacaaatag tggatttttg aataaatgca tcatgatgac aggatcttta gctctttttt
     8161 cggcaaaaac aaaaatacct aacattacga tactacctag cagcaaagca gaaacaagcg
     8221 gactcattcc ttcgtctcct aataattgga accctaacag caatgagaca agcatggcca
     8281 tcaaactaaa actaccaaaa atatccattg gtttagactc atgtatgatt tttggttcat
     8341 ttaaatatag ccaaatcaaa ataattaata gaattccgat cggcacattg ataaaaaaga
     8401 tccagtgcca gctgatcgtt tcaacgatga atcctcccgc aagcggtcca aaaacactgg
     8461 caatccccca ggctgaacta ttcaaaccta aaacttttgc tcgcttttca atagaataaa
     8521 gatcgccgat gatcgtcaaa gctactggca tcattgcccc agcacccatc ccttgaatcg
     8581 cacgagcagc aatcaaccca atcatgcttt gagataaacc gcaaaataac gaaccgataa
     8641 taaacaataa tgtcccaaaa ataaagattg gtttccgtcc cactttatcc gcaagttttc
     8701 cgtaaatcgg agtaagcata gcgtttgtca gcaaataaat cgaaaatacc cagttcatga
     8761 tttccattcc atgtaatgaa ccgacaatcg ttggcatagc ggttgttacg attgtacctt
     8821 caatagccgt catgaacgtt gcaatgaaaa tactcactgt gaccaaagta acatttgttt
     8881 cttttttcat cttattcctc cctttcatta tgacaggatc taaaaataga taaataatta
     8941 ctatcttttt caagaggtta actgccataa aaaaagctgg aacaaccacc acttggcgat
     9001 cgttccagct gaatataact tattttgcta aacttgcttt caacgcatct actttgtccg
     9061 tatgttccca aggcaaatct acatctgtac gaccaaaatg accgtaagca gctgtttgtt
     9121 tgtaaatcgg acgacgcaaa tcaagcatct cgatgattcc tgctggccga agatcgaagt
     9181 tttctctcac tgcagcaatc agttcttctt ccggaaccgt tccagtaccg aaagtattga
     9241 ttgagatcga aacaggttga gcaacaccaa tcgcataagc tagctgtact tctgctttgc
     9301 gagcaagccc tgctgcaaca atgtttttag caatataacg tgcagcatag ctggcagaac
     9361 ggtcaacttt tgtcgcatct ttaccagaaa aagcaccgcc accatgacga gcataaccgc
     9421 catacgtatc aacaatgatt tttcttcctg ttaatccggc atccccttga ggaccaccaa
     9481 tgacaaatcg gccagtcgga ttgatataat atttcgtttg atcatctaat aattcagctg
     9541 gaataacttc cttcactact ttttcgatga catcatgacg aattgtttca ttatctactg
     9601 catcatcatg ctgtgttgaa ataacgattg tatctacgcg ttccggttgt ccttgatcat
     9661 catattcaac cgttacttga gattttgcat ctggacgtaa atacgttaat tcatttgatt
     9721 tacgcaaatc tgccaagcgt cgtaccagac gatgactcaa agcgattggt aaaggcatca
     9781 attctggtgt ttcatctaca gcaaacccaa acattaaacc ttggtcacca gcaccaatct
     9841 catctaatac gtctttttta tcttcatccc ggatctcaag tgcttcatca actccttgag
     9901 caatatcagg agattgttca tcaatcgcaa ccaatacagc tgctgtatct ccatcaaatc
     9961 cgaattttgc acgtgtataa ccaatttctt ttattgtttc ccgtacgact ttttgaatat
    10021 ctacatatgc cgtcgtagat atttctccaa atactaaaac aagaccagtt gttacagacg
    10081 tttcacacgc tactcgtgct gttggatctt gttttaaaat tgcatccaag attgcatcac
    10141 tgatttggtc agcaatttta tctggatgtc cttcagaaac agattctgat gtaaataagt
    10201 gtctttctac cattataatg attcccccta gttaaattcg gttacaaggc atctccgtcc
    10261 tgcggatcct ttcgggaact tgcaacgaac caagtatagc agataaaaca gttttctgct
    10321 cgtatttttt catttttttg cttaaaaaat ataaaaatat tttacattag aactgtccaa
    10381 aaaagcttag cacaatcatc cataaagata actctgctaa gctttttaaa atcattcttt
    10441 cagataaaaa gaaaaagata ctcaaaaagt atcttccatg acccgtacgg gattcgaacc
    10501 cgtgttaccg ccgtgaaagg gcggtgtctt aaccgcttga ccaacgggcc aataaaataa
    10561 atgggcctaa atggactcga accatcgacc tcacgcttat caggcgtgcg ctctaaccag
    10621 ctgagctata ggcccataaa aactaaaagc gggtgacgag aatcgaactc gcgacaacag
    10681 cttggaaggc tgtggtttta ccactaaact acacccgcat aatggcgcgg gacagaatcg
    10741 aactgccgac acatggagct tcaatccatt gctctaccaa ctgagctacc gagccaaacg
    10801 gttccgacgg gaatcgaacc cgcgatctcc tgcgtgacag gcaggcatgt taacccctac
    10861 accacggaac caatgttttt agtttttaat tgcgggagca ggatttgaac ctacgacctt
    10921 cgggttatga gcccgacgag ctaccagact gctccatccc gcgataatat gaaaaaggag
    10981 gataagggat tcgaaccctt gcacggtttt acccgcctga cggttttcaa gaccgttccc
    11041 ttcagccgga cttgggtaat cctccatgta aaactatatt aaattaatga cccgtacggg
    11101 attcgaaccc gtgttaccgc cgtgaaaggg cggtgtctta accgcttgac caacgggcca
    11161 ggtaaaacaa aatattgaaa cggagaagga gggatttgaa ccctcgcgcc ggtttcccga
    11221 cctacaccct tagcaggggc gcctcttcag ccacttgagt acttccccaa aaccaataag
    11281 attttgtact aatgggccta aatggactcg aaccatcgac ctcacgctta tcaggcgtgc
    11341 gctctaacca gctgagctat aggcccataa aaaactaaaa gcgggtgacg agaatcgaac
    11401 tcgcgacaac agcttggaag gctgtggttt taccactaaa ctacacccgc atggcggttc
    11461 cgacgggaat cgaacccgcg atctcctgcg tgacaggcag gcatgttaac ccctacacca
    11521 cggaaccagt gtttttagtt tttaattgcg ggagcaggat ttgaacctac gaccttcggg
    11581 ttatgagccc gacgagctac cagactgctc catcccgcga taatatgaaa aaggaggata
    11641 agggattcga acccttgcac ggttttaccc gcctgacggt tttcaagacc gttcccttca
    11701 gccggacttg ggtaatcctc catatgaaac ttacaacgcc acaatggacc ttgtaggact
    11761 cgaacctacg accggacggt tatgagccgt ctgctctaac caactgagct aaaggtccag
    11821 gctcttaaaa atcgcggcgg aggggatcga acccccgacc tcccgggtat gaaccggacg
    11881 ctctagccag ctgagctaca ccgcgaaaaa gatattcatt atttaaatga aatggagcct
    11941 agcgggatcg aaccgctgac ctcctgcgtg caaagcaggc gctctcccag ctgagctaag
    12001 gccccgtgtt taaatttata attatcggga agacaggatt cgaacctgcg accccttggt
    12061 cccaaaccaa gtgctctacc aagctgagct acttcccgtt taattaacgc gcccaagagg
    12121 agtcgaaccc ctaacctttt gatccgtagt caaacactct atccagttga gctatgggcg
    12181 catatttgaa atgccgagga ccggaatcga accggtacgg tgatcactca ccgcaggatt
    12241 ttaagtcctg tgcgtctgcc agttccgcca ccccggcgcg attgctttgg caaagcggaa
    12301 aacggggttc gaacccgcga cccccacctt ggcaaggtgg tgctctacca ctgagctatt
    12361 tccgcgtgtt taaagatgcc ggctaaagga cttgaaccct cgaccctctg attacaaatc
    12421 agatgctcta ccaactgagc taagccggca tgtaaaactt aaattatgcg ggtgaaggga
    12481 cttgaacccc cacgccgtaa ggcgctagat cctaaatcta gtgcgtctgc caattccgcc
    12541 acacccgcaa aaatatgagc cgtacagggc tcgaacctgt gaccctctga ttaaaagtca
    12601 gatgctctac caactgagct aacggctcat atggaggtta acgggatcga accgctgacc
    12661 ctctgcttgt aaggcagatg ctctcccagc tgagctaaac ctccatggat aactt
//
LOCUS       DARBFO010000075        12540 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000075 DARBFO010000000
VERSION     DARBFO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.076"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            97..570
                     /locus_tag="SCK51_002205"
     CDS             97..570
                     /locus_tag="SCK51_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEG1252794.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            579..806
                     /locus_tag="SCK51_002206"
     CDS             579..806
                     /locus_tag="SCK51_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1252795.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1442..1813
                     /locus_tag="SCK51_002207"
     CDS             1442..1813
                     /locus_tag="SCK51_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252796.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2069..2311)
                     /locus_tag="SCK51_002208"
     CDS             complement(2069..2311)
                     /locus_tag="SCK51_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1252797.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2343..3245)
                     /locus_tag="SCK51_002209"
     CDS             complement(2343..3245)
                     /locus_tag="SCK51_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1252798.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3258..3446)
                     /locus_tag="SCK51_002210"
     CDS             complement(3258..3446)
                     /locus_tag="SCK51_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEG1252799.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3460..4023)
                     /gene="amaP"
                     /locus_tag="SCK51_002211"
     CDS             complement(3460..4023)
                     /gene="amaP"
                     /locus_tag="SCK51_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEG1252800.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4061..4954)
                     /locus_tag="SCK51_002212"
     CDS             complement(4061..4954)
                     /locus_tag="SCK51_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1252801.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5032..5955)
                     /locus_tag="SCK51_002213"
     CDS             complement(5032..5955)
                     /locus_tag="SCK51_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1252802.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6004..6354)
                     /locus_tag="SCK51_002214"
     CDS             complement(6004..6354)
                     /locus_tag="SCK51_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1252803.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6387..7289)
                     /locus_tag="SCK51_002215"
     CDS             complement(6387..7289)
                     /locus_tag="SCK51_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEG1252804.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7282..8139)
                     /locus_tag="SCK51_002216"
     CDS             complement(7282..8139)
                     /locus_tag="SCK51_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEG1252805.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8473..9282
                     /locus_tag="SCK51_002217"
     CDS             8473..9282
                     /locus_tag="SCK51_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1252806.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9322..9819
                     /locus_tag="SCK51_002218"
     CDS             9322..9819
                     /locus_tag="SCK51_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1252807.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10204..10277)
                     /locus_tag="SCK51_002219"
     tRNA            complement(10204..10277)
                     /locus_tag="SCK51_002219"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10241..10243),aa:Ile,seq:cat)
     gene            10464..10787
                     /locus_tag="SCK51_002220"
     CDS             10464..10787
                     /locus_tag="SCK51_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252808.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            10951..11205
                     /locus_tag="SCK51_002221"
     CDS             10951..11205
                     /locus_tag="SCK51_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252809.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            11275..11520
                     /locus_tag="SCK51_002222"
     CDS             11275..11520
                     /locus_tag="SCK51_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1252810.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            11596..11961
                     /locus_tag="SCK51_002223"
     CDS             11596..11961
                     /locus_tag="SCK51_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252811.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
BASE COUNT         3616 a         2333 c         2139 g         4452 t
ORIGIN      
        1 aaacgatata tttttattgt ttttcgataa aaaatagttg ttttacaata attattctgc
       61 tatattttag ctatcaaaga taaaagaggg ataaaaatga aatttaaaac attgctatta
      121 aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact tttttatact
      181 cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt atttgtttct
      241 gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt gaaactattg
      301 aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact aaaaaaaatc
      361 gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa atttatgaaa
      421 gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt cgtattcatt
      481 ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca tgcgatttta
      541 cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc gttaatttag
      601 atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa gtgggcatta
      661 cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt ttttccactc
      721 tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc tataaaaatg
      781 actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat aaaaaataat
      841 ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc agatttttta
      901 tttcgattga ataccgtttt atctataaat aagatatgta ctgactcata caaagggggc
      961 cttttcttct ccatgtacta tcacttttcc tgatacaact ggtttcctgt tacgttgtag
     1021 ttggatctgc ttttagtata atactttatt cattccacca gactgatagc agtttgagca
     1081 gtctaaataa tcatgtagta ttacaggcca ttctgtttca taccttgaag tgcttaaaac
     1141 ttcagctaat ggcccattgt gttaactctt actccattct tttttagtaa gctcccatat
     1201 atactgtaaa gccttctata tattatgtca tgttgctttt taacatttac tatgtagttt
     1261 tgataaaatg atttgaataa cagtgatgga caattctttt ttatttttaa cattattttg
     1321 ataataatga ttatttattt aaaaatacat tagataacat ttgttacgac acgaaaaagc
     1381 ttcacaaaca aatatcgtta ttttaaaatg agggcgaact cacacaatta ggaggaatag
     1441 tatgccattt attgtttact tttttctttc attactcgtg atctatattc cattaccaac
     1501 atttttcctg ctcgtcaatc gtctagcaga gcaacatgat acgattgctg ctcttacgaa
     1561 actttgttta cttctatttg tttgtctaga ttactttacg agctattatt ggctaaaaca
     1621 gacaagagta aaaaagagat attatttctt ccttgtcttt ttcaatctct ttgagtttct
     1681 gatccattgc tttttacttc atcagtatca tacgatccat ttgtggatca tctgggtttt
     1741 caaagtggtc gtattatttt gtatgttcac ctttcctatc tcaagaaaat ttagggaggc
     1801 attgttttat taaagaagct tctctgtatc aataaagaca catttaagga aaaactttta
     1861 aaaatctaat tgggaatttg cccgcataaa taagaggctg tgacaaaagt cttgtcacag
     1921 cctcttattc cgtataaaca gtgttcaaaa gcttgcatct tcgataatat gcccaaccaa
     1981 atgaaaaata tgagaagcta tttttgtttg gctgttctta ttacttgatg aagaacagct
     2041 ttttcacgac ctctttttaa aagtcttttt acagttttcg agcaaagaat gatacgatta
     2101 gtactaaaat gatggcacca atcaatgatg gcacgattgc cattcctgca atacttggtc
     2161 cccaagaacc taataatgct tgaccaagtg ctgatccaac taaacctgct acgacatttg
     2221 caataatccc catcgaagaa cctttattcg tgattgctcc tgcaattgct ccgataattc
     2281 ctccgacgat aagtgaccaa ataaatccca tgtttcgttc ctcctaatgt tttacttcct
     2341 tattactgaa cccgtggttc tgtattttct tttacttttt gtgcgccgct tccagctgct
     2401 gattttactt tatctgtttg attcgaagca aattcaccgg tttttttcgc tgcgtcagtt
     2461 actttgtctt gcaccgtttc ttgatctttt tcaaattctt cttttgtttt gatgtccact
     2521 acgttagcgt tgacttcgat cacttctaaa tgtgtcatat tttttacttc tttgccgatt
     2581 acttcttgca tttcttcaaa gattttttcg atatctttgc catattccac aatgacatcc
     2641 atatcaactg ctacttgttt tttaccgact tctgtttcga tcccagccgt tacattatct
     2701 gtattcacaa gttttcctgc cacattagag aagaaaccac cgtttgcaga taataaaccg
     2761 tctacttgtt caattgcgat tccgatgatt ttttgcacaa ctttatcttc ataattcagc
     2821 tcaccgcgca ttgaatcttc ctgttcagaa tcatgctttc cttggttatt tgtatgatca
     2881 gaaccaagtt ttccttgtcc gtttgtgtgg tcagaactaa gtttttcttg tgctttcgca
     2941 tggtcagaag gatgtttttg ctcttccaca ggtttagaag gatcgattgc atcatgttgt
     3001 tctggaactt tagattctgg agttcctgga tgatgaggtg catgcttttc tgcatcatgt
     3061 ttcttctctt ctactccttg ttgaacgtta gggactactg gatctggatg tgcagatact
     3121 ggtgctgttt tgttcttttc atcgttcaca ccatttgatt ggtttggtgt cgttgggtct
     3181 ggatgtgcag acactggttt ttggttgttc attttattgt tattattttt gttattgtta
     3241 ttcatggatg tttcctccta agatttaaaa tagtgatcta agattttcgt ttgtttcaga
     3301 taaaaaccga tagcgacgcc gatcgctgcc atgatcaaga tcaataaggt cttgaaaaag
     3361 cccacggtga tccataaaac ggctaatatc aatccaagta ccccaaagat gatagggagc
     3421 ttgtatgtcg aaaatagttc tttcattttt ggtccccccc tattctactc gtttgcggtc
     3481 attgcttcgt ttagctggtt cttcaaagcc tgtgtatttc accgatactt tcactggctc
     3541 ttttgttccc aatatgtgtt gtacttcatt ttcgatttct ttcatcaatg ttcctgtttt
     3601 accaatgagt gctgaagttc gtttcaactc cccttttaca ttgacggcga tcttgttttt
     3661 cgttgctttg acatttactt ttggtgaatg gatgaattca tcttcatgca agtgagaacg
     3721 aaccattcct tcaattgctt ttttatccaa cgtcaactcc ccatcaccat gtttcagtag
     3781 aaagtttccg attgtttttg gataaaacag gaaaacaagt aacgtgataa ttgtcaaaat
     3841 agcaaatgca acggcaagcc aaaatacata ttgttgcacg tatgaattag tgatggaaaa
     3901 gaatcgaaac ttctctagct gaaaaggcaa tggataggtt gcacttaata gtatcgcaaa
     3961 caaaagcaca gatagcaaaa gtaatgttcc gatcactccg atcgttttta cacctttatt
     4021 cattttcgca cctcctcata ggggtgaaac gatcaacttc ttataagttg atcgcttctc
     4081 caccagttac tccgtaaatt tgggctgtaa cataacttgc tttattcgat gctaagaaaa
     4141 cataaactgg tgctaattct cctggctgac ctgcacgttc tagtaaactt tgctgaccaa
     4201 attcagggat cgaatcagaa ggttgtcctt cgtctagttg taatggtgtc cagattggac
     4261 caggtgctac accatttaca cggattcctt taggtgcaag ctgttttgct aaaccgatcg
     4321 taaagttagc gattgaagct tttgttgctg cgtaatctaa taaatgttcg cttggattga
     4381 atgcttgaac agaagttgtt gttacgattg ctcctcctgc tgggatatgc ggaacagctt
     4441 ctttcactaa agcaaacata gcgatgatgt ttatcataaa tgtgtctttg acttgctcaa
     4501 gtggtaactc agcaatagac tgacaagaaa tttgctgtcc tgcgtttagt accatcgtat
     4561 ctagcccgcc aagttcactc acggctttat ctatgatttc tttaggtgct tgttcgtcac
     4621 gaagatcaaa aggtagtaag acaatcttac gtcctgcatc tttcacgtat tttgctactt
     4681 cctctgcatc tttttcttct cctgggaaga attgaagagc gatatctgcc ccttctctag
     4741 caaatgcaat cgctgctgca cgaccgatcc ctgaatctcc accagtgatt agtacacggc
     4801 ggttttctag ttgattattg ccttcatagg attcttctcc acaatcagga acaggaatca
     4861 tattctgctg taaagctggt gtatcctgat cttgtttagg aaatttctcc gtatggtaca
     4921 agtttcgcgg gtcgattaat tttggttctg tcattgctgt attcctccta gtttttaaat
     4981 tgtattgcca aaacaaattt ctttgtttga tacctgtttt aaatagattc tttaatttgt
     5041 ataatctttc cggtataaat ggtaacctaa aactactgct gctatcaaag agccaaagac
     5101 tacgagtata aagcctagag cagaactttt acccggtaat cctcctgtat tcattcccca
     5161 aatccctgcg aacaacgccg gaacggaaat gattaatgca gctgaatcta gatatttcat
     5221 gatatgattt agattattgt ccatcatccc gttgaataga tcaccaatac tctctaataa
     5281 atctcgatag atctcgatca ttttttctgt ttgtcgctgt ctcaaatgga catcatagag
     5341 tagctgttca tcgtctaaac gtttgatgaa agtttcagaa ttccataatt gatccattgt
     5401 ttcccgttga tctcttaagg tatgatcgat atatacgatg tctctttcaa gatctgcctg
     5461 tttatacagt tcttcatttt ccgttgtttt acgagctgct ttatccaaag cgtcaattcg
     5521 ctttttcatc tctcttagtt ctttcacgta atgggtgtaa atatctaata atgtatacgt
     5581 gatgatcttt tcaaacgagt ttaaatgatt tccatgcttt gtcagcagcc gatccacaaa
     5641 ttcggttttt ctatcgatac aagtaatcaa gagattatcg gaaagtacaa aagatattgg
     5701 cgttaatttt ttttcgatac gattatctga ctgatctaga ttaatcacta ccaaagaaaa
     5761 tggatgctct agtttcgtct cataaaggcg ctccatccga gaaacttctt ccggatattc
     5821 ggttcctacg aaaatatcat caggaagctc gaattcttcg attgcttgct caatctcttc
     5881 gggctttgca ctatcaagta cgagccaatt atattttttt tcatctgttt ctattacatt
     5941 cttttcttca tccacaaagt agtatctcaa tgtatacctc ctttttggac tttctcgaaa
     6001 ctattagctg aagaagcagt tctttaattt gatattataa tcgagtactg cgatttcatt
     6061 ttctaatgaa aagctgtcaa gctgtttgtt gaagtccatg ttcaatcgtt taccgaaata
     6121 attttgctct aatttagatg tatagatgtc caataatgga tgctcgattt tagtcgcata
     6181 ttctccaaat tccgaaacaa aaggaatgat ttgaaaaaag ctgccttttg caaaagcacc
     6241 tttgtccctt agaaatggat cggaatcatt gataagcgcg agtgcgattt ttgtattttc
     6301 cccaaccttt tcaattaacg tctctactgc atttttagac attgtaattt ccattcctac
     6361 tccccctctt cagttgttgt cttctattat ttaacaacta catctgtttt tggttgaact
     6421 tttgcatatt ttggatattt agggaattta gctacacaac ggaaagccgt catccctgca
     6481 tatttttcgt aatatttctt cgttggccct tcaaaatcct tgctccacgg tttcacatgt
     6541 cctgaaaaat ggataatgta aggattgttg cgagtttctt cgtattcacg ttcacctgct
     6601 gctgttggat gtttttttgc ttttgccatg atatagcctt gtgcattcca acgcggatga
     6661 agtggtaacc aacggtcatg taagatagca tttaatgcat cttggtcatg gaatcgtaat
     6721 ttttctggat tatgttcgat gaaatctaat actttttgcg taatattttc atctaaccat
     6781 tttttcacat tgatcagcat caaacctgag ttgaaataac gcattgattt agctgaaatt
     6841 ttcatttttt ccagtcgttg atggaaccca gcgtcttcaa cagctgctac gattgaatca
     6901 ccgaagtcaa gacgccataa tttagagata tcttgtaaag cgatcatgtc gcaatccata
     6961 tataagatac gttcaacttc tgtgccgcgg aacaattccg gaatcgcaat acgataataa
     7021 gctgtttctg gaatacgatc gctgattacc acatttgtaa agaattcttt attgattttc
     7081 aagaattcca catctgagtt cattcgtgcg tttttcactg agaagcgtaa aagctgttta
     7141 ctttcaaaat cgatatcatc gtcaatcaca tagaaccgaa caaatttaga cgggttgcaa
     7201 ttatctaata ctgagacaaa taatgcagct aaatgtggta cgaattttgt gttacaactt
     7261 gcgactactg ctatttcttt tttattcatt gatctcgcct actttcgtta gagtactgtg
     7321 tgctaatttt tttaagtata aattggtata cggatgatct ttcaacgtat tccaaggttt
     7381 atcatgtcct gtaaagtgaa cgatcgaagg tttttcattt ccttctttat actgacgttc
     7441 atatttttca tttggtgctg gatgacgttc aaaaatcagc gaagtctgca tgttccattt
     7501 tggatgaagt tgttcccaat cttcataaag tacagcattc aaagcatctt ggtcgtgata
     7561 gatgattcga tcgccatttt cactgaggta atggatagtt ttttctgtaa tttctttttt
     7621 attccattga tcaatgtcaa tcaccatcac tcctgaattg aagtacaagt catcggaatc
     7681 gattcctaat ctttccaaag ctttcacttg gcctggatcg ataatagcgc cgattgtttt
     7741 tccatttaac ggctcatcat atagttttac gatatcgtct agtactaaca catctgaatc
     7801 taagtaaagg actttcttat aatcctcttt agctaataag tttggtaaag aaattcttaa
     7861 gtaagcggta gttgtgatat ggtcactgac taggaagtct tcgtagatat ctttttccac
     7921 tgtcaggaat tggatacttg cattacttga atatttattt accgtttctt ccaatccttc
     7981 tttactatat tctgatagac catcgtcaat cacataaaat tttatgcgtc ttgttttatt
     8041 gcaattttct aatgcagttg cgatcatcac gctcaagtaa ggagcataat tttcgtctga
     8101 agcagttacc acaggaacta cgccgtatct cttctccatc aaaatcactc ctttttctaa
     8161 tctttttcta acctgttcta catctagaat attcctcatt tggttttaaa gcaaaatata
     8221 tgctttgttt tcattgaata ttttaaaatt tttttatcac aaaacgcatc ggtaagcgct
     8281 aactattcgt ttttcatggc tttttattca aaggtattga ccaaaaacta ctatatatag
     8341 ccaccttttt cttaagaatg ttttttgaaa aaattcataa tgacaaagca agttagtttc
     8401 atttttttaa attctcattt aatcttaagg taacaggaaa atagatatgt aaaaagaaag
     8461 taggaagata aaatgaaact agcagcgatt gatttagacg gtaccttgct tgatcgaaat
     8521 ggaaagatac cagaagaaaa tatccaagca cttcggaaat ttgatgaaaa tggcggaatc
     8581 gtaaccgtcg ctaccggaag gaacagcatt tctgctaaag atattttttc cgaattagga
     8641 atcggtggct atttgatttc ttccaatggt tcactgattg ctgaaatgaa ggatggaaaa
     8701 atggcgaaag ttctcagacg ttcaaaaata gatagcgata cactgagaaa agccttcttc
     8761 tatgctcaag aagcgaaaat atccatcatt gctagccgag aaacccaaga tgatcagctg
     8821 acattcgatg aatcaaatct agtcaaagat gacccttatt atgaacattt caatttacag
     8881 aaccattctt ttgatcaaat tttaaatatg ttggaagatc cttctcttag ctatcttaaa
     8941 ttagcattaa cagataagag tgaagaaaaa ctacagaagc tacaaagaga tctgcaagca
     9001 gatggtatcc aatcagtatt ctctgatccg catttcttag aaatcacgcc tacaggtatc
     9061 actaaagctc attcacttgt ttttctggcg gattaccttg gaatcgaatc gagtgacatc
     9121 atggcttttg gcgatcaaga aaacgacatt gctatgttga attacagcgg gttgagtatt
     9181 gcgatgggga atgcacagcg acaagtaaaa gaaattgcaa accaagtaac actgacgaat
     9241 gaagaagctg gtgttgctta tattttaaaa aacttctatt aaaaagaata aaatgacgat
     9301 atgaatgtag gaggaaagaa aatgttagtt attattgata tgcaaaatca tattcttgat
     9361 ccaaacagcg aattttatat tccagagtca gatcaattgg cagaacgtat tgctcaaaga
     9421 ttagctaaag cacgccaaaa taatgaatat attctcttca cacgggatat accagttgaa
     9481 ctgaaggaca aagaagaaga acgagaagat ttgcaattga tccctcttct ttctccacaa
     9541 gaaaatgaac gagtgattaa aaagtattac tttacgattc cacctgaaac tttagctgaa
     9601 atccaggaaa ctttatttga aagcaaagaa gaacaaaaag agatcgaagt agtcgggatt
     9661 gaaacaaact tgtgtgtact ctctaatctg ataggcctgc agagtgcttt tcctgaagca
     9721 gacttttttg ttgatccaat gttagtctcc agtagaaaac acggagaatc cgctttagag
     9781 ctgttgaaag atttcaacgt ctctatcgtc gaatcataaa aatatctatc ttcatttacg
     9841 atatttaaca cacaataaaa aaagcacgca gaactgtttt agtcctacgt gctttttcta
     9901 tcaaagtaga ttggcttttt tatgacacag agtgaaacgg caggctcacg acctctttct
     9961 taaatggtgt tcaatcagct gaccctcggt attagcttaa caaacggcaa aatatgagga
    10021 actatttttc cgtttgttaa gctaatactc aggtcaaaac gctgatttca caacctcttc
    10081 cttaaacggt attcaaaagc ttgcatctgc agtaataaga acagccaagt aaaaacataa
    10141 gaagctgttt ttacttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc
    10201 ttatgtacct tgtaggactc gaacctacga ccggacggtt atgagccgtc tgctctaacc
    10261 aactgagcta aaggtacatc ataaataaaa tttatttttg ttacttttca actatagcac
    10321 gaatgacaaa ctctcacaag tttcaaaata taagtaaaat aatattgtta ttcttatttc
    10381 ttttacttgt gtcataacta ttttgttact ttaattttta tttgcttatt ttagaaaaaa
    10441 tgttattctt ttatttattc tcaatgttag gagtgattat tttgccattt attgcgattt
    10501 tattcgattt agttgcggca gcagcttatt ttctccaatt aaattatcaa acctcaggaa
    10561 ttctgtttat tggaatgatt tttcaaggtg tgattaccct tttgcttctt gtcatgatgc
    10621 tcagctataa aggaaaaaga tatgcccgta ttcaaacaaa agtatttgtt aaatatgttt
    10681 ccattcgcta tgggatcatc attctttcct ttttagtgaa tgcgatcgtc ctctttttat
    10741 atgtattgaa ttacttagat atcaatccat tgattttttc tcgatagata agacacattc
    10801 agcggaacag ttcctgctta tcagcagaaa actgttctgc tttttcattt ttcattgatc
    10861 tattcttctt ttattttcct agcatttcat ttgtgatgta tgcaataaca tgttatcttt
    10921 tattatatga atcgttgagg agtccgttac atgattacgt tggtgagagt tttgttttgg
    10981 gtgcctgcgg tcgcactggt cgcaagtatt gtttatctta tgaattggaa caaagaacgg
    11041 ttttatttag ctattttgac tcttccggct atctacttta tgtggaaagt ttttaattac
    11101 aactactttg aacccgatag tgtttttata gaagaattat caggactggt tttgtcttta
    11161 ttgatcgtca ttctttattt gatacgttta aataaaaaac attgagtact attgattgca
    11221 tgtgtagctt tctatagtca ttataactat tctactataa tgaaaggtga ttaaatgagt
    11281 atcgaagaag ctattttact ggatacttta cgttgtcgtc tgaatttctt aagaactggc
    11341 cgatctttat ctcaagtagc tgtttctagc gcgttaagta ttcctttatt caaatatcgt
    11401 aagtacgaag aaggagaaga aattccaagc atcattgaat taatctatat cgctgacttt
    11461 tacgatattt ccgttgatta cctaattggt cgctcagaaa cagcaagcaa actgaagtga
    11521 ttttttccag ccttggtgct ggttttttca tttaacgtct aaggttaaca atctatatat
    11581 aaaggagaca tttctatgcg attatggcat gaaaaattga tttcaaagct tcctcgtcaa
    11641 cagcttttag gacaacatcg ggaatgctgt gcattaagag gaaatggctg gggaaagaaa
    11701 catgctactg tggattatgt ttttcactat tctccttata gattattcca atatcatcag
    11761 cttgtcatgg atgaaatgga aaaacgtggt tacagcccag caccagagtg gaaaaatcct
    11821 atgtatcgtg gcaaatcatg cgaagcattt tttgatttac ctactgtaga gcgcaacgtg
    11881 ccgatttatc cggaacacga tgaagtttat ctagcagaat gtttagcaaa tttggagcaa
    11941 aaaggaattt atcttaccta atataactag aaaagccttc tatctcctct tacttcatta
    12001 aaaagcttta accatcttag atcaagctaa aatgtcgtaa gttttagggt tatcttcatc
    12061 ctcacgtgca aaactaaaag cgtactctag ttttggttca tcgccaactt ccctagacat
    12121 aaatgaacat ctcctgacgt ggtttaaaag taccgcatct tcgacgtctg tatacgatgc
    12181 acagcacaga ttgcttcttt tgatctttct tttgattttt ctgttgcttc ttctacacct
    12241 tcttcgatta ctacatcgat gcttacaatt gcttctgcct aatattccca tgcatcgtcg
    12301 atttaagcct ctgtagtcac tgtgatcgtt tccacatttt aactcctctg tcgtggcttc
    12361 agttgatttt gtgatcagtt cagttgctaa gccagagtca gaacaacctg ccattgctag
    12421 cgttaatatt gatagtaaaa atagtttttt tatcataagg ttccccccct gaaaatagac
    12481 taaaataata acgctttctt tttttgtttt cagacgaaaa ttaataattt ctaaatattc
//
LOCUS       DARBFO010000076        12210 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000076 DARBFO010000000
VERSION     DARBFO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.077"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(82..639)
                     /gene="frr"
                     /locus_tag="SCK51_002224"
     CDS             complement(82..639)
                     /gene="frr"
                     /locus_tag="SCK51_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEG1252812.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(642..1364)
                     /locus_tag="SCK51_002225"
     CDS             complement(642..1364)
                     /locus_tag="SCK51_002225"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEG1252813.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1500..2381)
                     /locus_tag="SCK51_002226"
     CDS             complement(1500..2381)
                     /locus_tag="SCK51_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEG1252814.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="SCK51_002227"
     CDS             complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="SCK51_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEG1252815.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3621..4100
                     /locus_tag="SCK51_002228"
     CDS             3621..4100
                     /locus_tag="SCK51_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEG1252816.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4317..5408
                     /gene="phnW"
                     /locus_tag="SCK51_002229"
     CDS             4317..5408
                     /gene="phnW"
                     /locus_tag="SCK51_002229"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEG1252817.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5401..5529
                     /locus_tag="SCK51_002230"
     CDS             5401..5529
                     /locus_tag="SCK51_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEG1252818.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5533..6078
                     /locus_tag="SCK51_002231"
     CDS             5533..6078
                     /locus_tag="SCK51_002231"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEG1252819.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6532..8223)
                     /locus_tag="SCK51_002232"
     CDS             complement(6532..8223)
                     /locus_tag="SCK51_002232"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEG1252820.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8643..9590)
                     /gene="arcC"
                     /locus_tag="SCK51_002233"
     CDS             complement(8643..9590)
                     /gene="arcC"
                     /locus_tag="SCK51_002233"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEG1252821.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9705..10724)
                     /gene="argF"
                     /locus_tag="SCK51_002234"
     CDS             complement(9705..10724)
                     /gene="argF"
                     /locus_tag="SCK51_002234"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEG1252822.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10815..12044)
                     /gene="arcA"
                     /locus_tag="SCK51_002235"
     CDS             complement(10815..12044)
                     /gene="arcA"
                     /locus_tag="SCK51_002235"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEG1252823.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3343 a         2528 c         2203 g         4136 t
ORIGIN      
        1 tttttttaat ttttaaataa aagaaatata aataaaaaca gaggaagcgg aatgatccgc
       61 ccctctgttt ttatgatttt attaaacttc caataattct ttttctttat cagcagcgat
      121 cttatctact tctttgatgc tgttatctgt caatgtttgt acgtcttttt ctaatccgcg
      181 caaatcatct tcggtaatat caccattttt ttgttgtttt ttatattcat cgatcgcatc
      241 acgacggatg ttacgaatcg cgatttttgc attttctgct tcttttttaa catctttagc
      301 taattctttt ctgcgttctt ctgtcagttg cgggataact aagcgaatga catttccatc
      361 attggttgga ctgattccga tatcacttgc cataatagct ttttcaacat cttgaagaat
      421 gctcttgtca aaaggtgtga tcattaaaac acgtgcttct ggaatttgga ttgatgccat
      481 ttggttcaat ggtgttggcg caccgtagta gttcacagta atacggtcca aaaggctagc
      541 gttcgcacgg cctgcgcgga tttgcccaag ttcacgttga agattttgtg ctgctttttt
      601 cattttttct tttgcttctg tcataattgc atctgccatt tttatttccc ccttacagtt
      661 gttccgatat tttcgcctaa gattgcacgg cgaatatttc cgtgttcatt taaattgaag
      721 acaagcagtg gaatatcatt atccatgctt agtgaacttg cagttgaatc catgacttgt
      781 aatcctttgg caataacttc cagatgagtc agttcttcaa attttactgc atttgcatct
      841 actttcggat cagcagaata aacaccgtct acattgtttt tagccatcag gataacatct
      901 gcgccgattt ctgccgcgcg taatgctgct gttgtgtctg tcgagaagta cgggttgccg
      961 gtaccgcctg caaagataac gatccgtcct ttttctaaat gacgttctgc tttacggcga
     1021 atatatggct ctgcgatttg acgcatttca atcgaagttt gcacgcgtgt cggtacaccc
     1081 acgttttcca acgtatcctg taaagctaag gcattcataa ctgtagctaa catacccatg
     1141 taatcagctt gagcacgttc catgcccatt tgtgctccga tttgtccgcg ccagatattt
     1201 ccgccgccca caacgattgc catttcaacg cccagctcgt gaacttcttt gatttcttgg
     1261 atgatttctt tgattactgg tggtttgatt ccaaatcctg cgtccccagc taaagcttct
     1321 ccactcaatt ttaagactac acgctgatat ttcggtgtga ccatgttcat tcctccaata
     1381 ttttctattg ccattttacc atacttagat gatttcgaca ctctaagact ggtttttctt
     1441 gttttgctta aaaaaaggga gcgcacaaac cgtgcgttcc cgtctgttat tcaacagaat
     1501 tattttttca cttggctcat aacttcttct acaaagttat cttcgcgttt ttcgatacct
     1561 tcgccaactt cgaaacgtac gaatgtttta acagttgcgc cttttgatgc aacgtatttt
     1621 tcaacagtca tatcaggatc tttaacgaat ggttggtcca ccaaagcgat ttctgctttg
     1681 aatttcttca agcgtccttc taccattttt tcaacgatat tcgctggttt gccttcgttc
     1741 aatgcttgtt ctgtaagaac agttttttca tgttctaatt cagcttctgg aatttgtgat
     1801 tcgtttacat aacgaggatt gatagctgct acgtgcatag ctacgtctct tgcaactgtt
     1861 tcgtcagttg ttccatctag aacagtcaat acggcaatac gtccgcccat atgcaagtat
     1921 ccaccaaatg cagcattgtc gtctttttct acaacttcaa agcgacggaa gctgattttt
     1981 tctccgataa cttgagttgc ttcgatcaag tcagattcaa ttgtaccttt atctgttttg
     2041 attttcattg cagcttccat gtctgctggt ttgttttcag caacaagttc tgcaatttct
     2101 tttactaaat cttggaacat ttcgttttta gaaacaaagt ctgtttctga gtttacttca
     2161 acgattgctg cagtgttacc tttcacagca actgaagcca atccttcagc tgcaatgcgg
     2221 tcatttttct tcgcagcctt tgccattcct ttttcacgta aaagatcaac tgctttttcc
     2281 atgtcgcctt ctacttcaac taatgctttt ttcgcatcca tcattcctac gccagtcatt
     2341 tcgcgtagtt cttttaccat tttagctgta acgtctgcca tgtttaactc ctcctgtgtg
     2401 tttttctttt taaaaaaagc tgcttcaaag gagagtgagg cttgtcgccc agcctttgaa
     2461 acagctccat cattcaaaat tattctgctg aagaattgtc gccttcaaca acatctacga
     2521 tttcttcgat tgatgttgct gtgttttctt ctgtgaaatc ttcttcaact acttgatctt
     2581 caccttggtt tccttcgatg aaagcatcag ccatttttga agtaatcaat ttaacggcac
     2641 gaattgcatc gtcgtttgaa gggattacta cgtcgatttc atctggatcg cagtttgtat
     2701 caaccatagc tacgattggg atatttaatt tgtgtgcttc ttgaacagca atacgttctt
     2761 tacgtgggtc aacgatgtac attacatctg ggattcttgg catatcagcg ataccgccta
     2821 agaatttttc aagacgttca cgttctttgt tcaaaccagc aacttctttt ttaggtagaa
     2881 cttcaaaagt tccatcttct tccattgcat tgatttgttt caaacggctg atacgttttt
     2941 ggatagtatc ccagtttgtc aatgttccgc ctaaccaacg atggttaacg aagtattgtc
     3001 ctgcacgagt tgcttcttct ttgattgctt cttgtgcttg tttttttgta cctacgaata
     3061 atgctacgcc gccttcttca gcaacgctct tcatgtaatc ataagctgca tctactaatt
     3121 taactgtttt ttgcaagtca atgatgtaga ttccgtttct ttctgtgaag atatatttct
     3181 tcatttttgg attccagcga cgagtttggt gaccaaagtg tacgccggct tctagtaatt
     3241 gtttcataga aattactgcc atgtttgttt cctccgattt ggttttattt ttcctcttct
     3301 tatcttctac cttgccggca aactttttca agcacctacc gacaatccaa taagaatgtg
     3361 gatttgctga tgcataaagc accctcagct attatacaga caaaagatcc gtatcgcaag
     3421 agcaaatata cttttctgct caacattcaa tagtttacct gaaagataaa aagaattcaa
     3481 gaaaaacagt agatttctct ttgtttcttt attatccttt ttccataaaa taattttagg
     3541 tataaaagac gaatattatt gggcaatcct tctttttttt gttatgatac atgtgaaatc
     3601 tttcgtaaag gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt aaacaagtca
     3661 ttgaagaatt cccgatccgt acgcaagaag aactattaaa caaacttggg cattatggcg
     3721 ttaccgccac gcaggcaacg atttctcgag atatccgtga tctaaaaatc gtgaaggcac
     3781 ctgatgaaaa tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact cctgagacta
     3841 ggactgagga agaggatcgg ctggttcaaa tgattgaaga cattgtattg aaagtcgaac
     3901 gagtccactt tttgacgatc gtcaatacct tgccagacaa tgctcattta tttgcatctg
     3961 ttttagatga gatcaaacct ccctctattg tcagtacaat tgctggattc gatacgatca
     4021 tggtcatctc ggaaaatgag gaggacgcaa aacgagtaga agaatttctt caccatccaa
     4081 ctgaagtatc acttttttaa tcagagcacg tattcctaat atggaatgcg tgttttttta
     4141 ttttcaatat tttccttatt gaataaacct ttacactctt tacaaaaata catcattttt
     4201 tattcaaatt tttcaacacc ttgattgaat cacaataatc atcatttata ttggatgtta
     4261 acaagagcag cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga aatcatatgt
     4321 ataaattatt aacttctgga ccattgacga cttctgaaac agtgaaaaat gctatgctga
     4381 tcgatcattg tacttgggat caagaataca aagaaattac acaatggata agaaaaaaat
     4441 tattggatct tgctcaagta tcggaaggaa tatacacaac ggtcttaatg caaggtagca
     4501 gctccttcgg ggtagaatcg gtattaagtt ctgtgatcag gccagaagat actgtactga
     4561 tttgttcaaa cggcgcatat agacaacgaa tgatcaacat gtgcgaatgt ctgaaactga
     4621 actataccgt ttatgaggta tcggaacatc aaatcctcca agctcatgtg attacacagc
     4681 tgattgaaga gaatccgtct atctccgcac tagcaatggt ccatagcgag actacttccg
     4741 gtattttgaa taatttggaa gagattgctg ctgtcacaca agaaagagat cttttgttta
     4801 tagtggacgc gatgtcaagc tttggtggga taccgatttg tgtagccgaa ctagggattg
     4861 attttttgat ttccagctca aacaaatgta ttcaaggtgt tccaggcttt tcttttgtac
     4921 ttgctaataa agcggaactg gaaaaacgaa aaaatgttag ccgaagtttg tcacttgatc
     4981 tatatgacca gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc acttccccta
     5041 cccatacggt tttggctttt caacaagcgc tgaaggaact agaggcagaa ggaggaatcg
     5101 aaactcgata ttcacgttat ctcgccaata atcatgtgct acgacaaaaa atgacagaga
     5161 tggggttcca gcctttcatt caagaatatc aggggccatt catcacctct tttctttacc
     5221 ctaaggactg gcgatttgat tttaatcatt tttatcagta tttaaagcaa aatggatttg
     5281 tgatctatcc cgggaaaatc agcgatcatg aatgttttcg tatcggaaat attggcgaga
     5341 tctatccgga agatattttg gaactgacag caattatcaa agactataag gagaacgtta
     5401 atgattaaaa ctgtaatttt tgattgggca ggaacaactg tggactttgg ctgtatggct
     5461 cctgtccacg cttttagaaa tgcttttcta gaaaaaggaa tccaactgac tgacaaagaa
     5521 atccgctaac cgatgggaaa attaaaatgg gatcatatcc aacagcttct ttccctccct
     5581 tctgtcaaaa aacaatggat agctatttat ggacaactac ctcaggaaaa agaggtggag
     5641 gaattgtatc aacaatttga acattatctg tttaaagatt tggtcaagca ttctaccttg
     5701 aaacccgaca cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa tattggttcc
     5761 acgaatggat ttaatgctcc tatgatgaca atcgtagcag aaaccgcaaa aaaagaaagg
     5821 tattcaccag acttcatggc aacttttgaa gacgtcgaag ggtatggtcg accgtttcct
     5881 tatatgatcc ataaaaacat gcagtacttc aaaaacaagt cggttgatga agtaatcaaa
     5941 gtcggagata cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt gacagtaggt
     6001 gtaattgaag gtagttcgct aatggggatt tcttatgatg agtaccaaaa attagagatg
     6061 gggaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagcc ggcgctgata
     6121 tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac atgaatccag
     6181 aatgactttt atacaaaata agaggttgtg aaaaagctgt cctgtatcaa gtaataaaaa
     6241 cagccaagca aaaatagctc ctcatatttt tcgtttggtt aggcttatta ccgcagatgc
     6301 aagcttttga acaccgttta ttcgaaaaaa aaaagggagc tgtgacacga cttttgtcac
     6361 agtcccctta tttgcaaaca aacagtgttt aagagacaac ctctccacta aacggtgttc
     6421 attcagctga ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc tattttctct
     6481 tttgtttctc taatactcgg atcagaacgc tgatttcaca ttctcttttt cttacatctt
     6541 atctggtgca tgaagtccta gcaaacgaag gtcttctttc aataatacag tcactgcata
     6601 aactagagct aagcgtgctt ctttttgttc atcatcagct aaaatcttcg tatgagcata
     6661 gtatttgttg aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg acggttcgta
     6721 tttttcccca gcagacaaga ccgtttcagg gtatttttga actaatttaa ccacttccca
     6781 gctggtatcg tcattcaatg catagtctgc tgtttctgaa gggacaaatc ctgctttttc
     6841 caacaggctg actgcacgag catgtgtata ttgcacataa ggtccagttt ctccttcaaa
     6901 tcgaacaact tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt tcaaatcgtg
     6961 gaaaatgact gccccaaccc caacttgttt agctacagca tctttgtttt ccaaatcagg
     7021 gtttttctcg ctgatttgtt ctttagcaga ttggattgct tcatttaata cttcttctaa
     7081 taagacgatc tttcctttac gagttgataa ttttttacca ccttgcgtga tcaaaccaaa
     7141 tggaatatgg tgcatgtcgt cagaccagtc aaagcccatt tccttcaaga cagctttcaa
     7201 ctgtttaaaa tgatagcttt gttcattccc tactacatat agagattgtt tgaagtcgta
     7261 agtacgttta cggtacaatg cagcagctaa gtcacgtgta atgtatagtg tcgcaccgtc
     7321 tgacttctta atcaacgctg gattcaggtc ataagcagat aggtcaacga tctctgcacc
     7381 tttatcctcg ttcagcaaat gtttttcttc taaaagtttg acgatttcat ccatcttgtc
     7441 attgtagaaa gcttcaccgt tcaacgaatc gaaacgtact tccagcagat catagatttt
     7501 attgaattct ttcatcgatt catcacggaa ccattgccat aattgaatag cctctttatc
     7561 gccttcttcc agacgtttga accaagcacg agcttcttca tttaattctg gttctgtttc
     7621 agctacttca tggaattgga cgtataaacg aagcaattca ttgattggtt ctgctttaac
     7681 agcttcttct gtaccccatt ttttgtaagc gacgatcagt tttccaaact gtgttcccca
     7741 gtcacctaaa tggttgatgc ggattggctg ataaccgatt ttttccataa tgaaaccgat
     7801 cgaattaccg ataactgtcg aacgcaaatg tcccattgag attggtttgg caatattagg
     7861 agaagacatg tcgattggta ctgttccttg attcccgatg ttgctatccc cataatgttc
     7921 tttttctttc actactgttt gcaaaacttt tttgctgata agttctttat tcatgaaaaa
     7981 gttcaagtaa ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga ttttttctgc
     8041 taaatctgca gcgatttgct gtggcgcttt tcggtaaact tttgctaatg aaaaggctgg
     8101 aaatgcaaca tctccgtgtt cagctgattt tggattttct agtaattgct cgacttgttc
     8161 cagtgttaaa tcatctttga cagcgtcata gacagctttt gcgacaagat ctttattatt
     8221 catctttatt cctcctaaag tgttcccttg attgttaaac aaaaaaaatc tctagcgacg
     8281 gcatacttag tatgccatca ctagagacga gatttcccgc ggtaccactc caattgtcct
     8341 aaaaaaggac cagctccatc tgatatcggt agacgaccgt ctgtttaaaa cagaattctc
     8401 caagtgcgct tcgttgtatc ttcttcattc ggcttccacc acttccgaat tcgctagcca
     8461 agaatgatac aagtactctc ttgttcaaca aatcttagtc ttattaaact ttcttgaatt
     8521 atatcagaaa aaaaaacgaa aggctagtcg tttcataaaa aaacaatcca gatggcctaa
     8581 aggagaagaa ataccacctg gatagctata aatcatttcg taaatacgaa agacttaaca
     8641 aattaatcag ctacgataat cgttccttct tcagaagcta aaagattctc gatattttct
     8701 aaagaagtga tgattgcctt agcgtttggt tttgcttcaa caaatgcaat agctgcttct
     8761 actttcggaa gcatgcttcc tggagcgaat tgcttttcat caatgtattg tttcatttct
     8821 gaaacagtaa cagtttctaa tttcttttga tctggttttt ggtaattgac atacacattg
     8881 tctactcctg tcaatacgat cagcaaatcc gcatcgatga tttcggctaa tttttctgaa
     8941 gcaaaatctt tatcgattac ggcttctcgt ccttctaatc cagtagctgt ttctacaaca
     9001 ggaatcccac cgccaccaac agagactgtg ataactcctt gatcgaccag cgtttcgatg
     9061 acacgtgctt ctttaatcga gatgggttta ggggaagcga ctacttttct ccatccacgt
     9121 ccagcatctt ctttgaatgt cacagtggag tcagcattca tttgttcttt ggcttcttct
     9181 tccgtgtaga atggaccgac tggtttactt ggatttttga atgaaggatc attttcatcc
     9241 acaataactt gagtgactaa agaaaccaca tctttatcga tccctttttc cttcagtact
     9301 tcgcccatgg cgttttgtaa ccagtaaccg atagatcctt ctgtcattgc aacacatgta
     9361 tctaaaggca ttgccggtgt tttctcagaa tcagcagctt gctgctgaat caataggtta
     9421 ccgacttgcg gaccatttcc gtgggagatg atcaattcat ctccttgttc gataaatttc
     9481 actaaatact ttgctgtttc cattaacgct tcttgttggg cttttgcact tgcatccgta
     9541 gacaagatgg cattgccccc taaagcgaca actacttttc gtttggccat ttcattcgtc
     9601 ctttcttcag ttctaccgaa ttttctagat aacaaaacga gccagcgttt tgtgattttc
     9661 cctcaatgct gactcgtttt ttcgtgagat gaatgtggtt atgcttaaac gcgagggata
     9721 aacaagttac ctaatgttgc tgccatgatc gctttgattg agtgcatacg attttctgct
     9781 tggtcaaatt gacgagcata tttgctgcgg aagacttcat ctgttacttc catttcagtg
     9841 atgccaaagc gttctttcat ttgttcaccg taaacagtgt ttgtatcatg gaaagctggc
     9901 aagcagtgca agaagatcaa acgatctgtg ttgtgtgttt tttctaccat ttccatattg
     9961 atttggtaag gtttcaacaa tttgatacgt tcttcgaatt tatcttcttc acccattgat
    10021 acccatacat cagaataaag gacgtctgct ccgtctacac ctttatcaac atcgtcggta
    10081 atcatcaatt gtgcaccgga tttttcagca aagccttctg ccattttgac gatttcttct
    10141 tcaggttgta attcttttgg tgcaacgata cgcatattcg ctcctaaaat tgcacctgtg
    10201 actaataaag agttagccat attgtttcga ccatctccgc agtaagcaac agtgatacct
    10261 tctactgttc caaagttttc ttggatagtt aaaaagtcag cgatcatttg tgttggatgc
    10321 cattcatcag tcaagccatt ccaaactgga acaccagaga attctgctag ttcctctacc
    10381 attttttgac tgaatccacg gaattcgatt ccgtcaaaca tacgtcctag tactttagct
    10441 gtatcttctg tggattcttt tttacctaat tggatatcat ttgcacctag atattcagga
    10501 tgtgcaccta gatcgatcgc tgcagtagta aatgcagaac gagtacgtgt agatgttttt
    10561 tcaaataaaa gcgcaatatt ttttccttct agatagtgat gtgggatacc acgttttttc
    10621 aagtctttca agtgttctga aaaatcgatc aaatattgta attcttcttt tgtaaagtct
    10681 ttttctgcta ataagcttct tccttggaat actgattctt tcatattaaa cattctcctt
    10741 tagattgttt ttatttaaga ttaaagagga aatcccgacg gcagctgaca gtaagactgc
    10801 cgtcaggaaa caaattactt caagtcttca cggacaagtg gttggctcat gcagcgtgga
    10861 ccaccacgac ctcgtgacaa ttcacttgaa ttgatttcta acactttgat gccatagcta
    10921 cgaagcaatt cgttcgatac ataatttcgg ttataagtga ctacaacacc tggagcgatt
    10981 gccaatgtgt ttgatccatc attccattgt tctcgaggtg caacgatctc atctccatta
    11041 ccagttggga tcagtacaag atcatctaat ccaagagcag ctttcatcgt ttcgtgtaag
    11101 ttatctgaat gagtaatctt gatatcatca ccatctggtt cgattgtaaa gacatccatc
    11161 ttaccgtctt tactttgaat tgctggatgg atagtgaatt tatcgtgatc taccattgta
    11221 aatacggtat ccaaatgcat cattgcacgg ttgttcggaa ttttgattgc caacactttt
    11281 tcaaatcctg aattttttgc gaataggtgg cgtgctaatt tttctaaagc tttcgcatta
    11341 gttcgttgag aaatcccaac agctactact ttatcgctta agatcaattc gtctccgcct
    11401 tcgatatgat ctggattttc acgatccaac catacttcga ttcctttatt tgcaaatctt
    11461 ggatgatgtt tcaaaatata ttctgtaaac atcgattcac gacgacgtgc ttcaaaagtc
    11521 atcttattga ctgtcatccc atttcccatt gatgcggaag ggtcgcgtgt aaagtacaag
    11581 tttggcattg ggtccatgta aaatggatag tcatcgtctg ctgacagatc atatagactg
    11641 ctagaacgta cttcgatatc ttttgtacga accccagcca tgattttatc taccatttct
    11701 tgcgtttcca ttgatagaag gaactcatga agaccctctc ttacagcatt cgatgcaata
    11761 tgagattcat ccaacatttt attcaagaat tgttctttga cattgcctgc gtcaatcgct
    11821 tctgccgcta atttttccaa gtaaagagtc tcgacgcctt cattttgcaa tgtcttcgca
    11881 aagttgtcat gttcttcttg agcaatcgga agatatggaa tatcatcgaa caacagacga
    11941 tccataatat ctggtgtcaa attttccact tcttgtccag gccgtttcag taaaactgtc
    12001 tttaatttcc ctatttcaga gaaaacgtga ataggtttat ccatgttgtg ttgcctcctt
    12061 ctttttgatt acatgtatag gatatcctcc ttttgtaatc actttcataa ttttgtctca
    12121 ttcaaaatga gacattcctc acattaatat tcgataatta aataattaaa cgtttttcta
    12181 tttttaagcg taatttattc attttttatt
//
LOCUS       DARBFO010000077        12180 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000077 DARBFO010000000
VERSION     DARBFO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.078"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(142..306)
                     /locus_tag="SCK51_002236"
     CDS             complement(142..306)
                     /locus_tag="SCK51_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252824.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            601..1929
                     /locus_tag="SCK51_002237"
     CDS             601..1929
                     /locus_tag="SCK51_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEG1252825.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2131..2964
                     /locus_tag="SCK51_002238"
     CDS             2131..2964
                     /locus_tag="SCK51_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEG1252826.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2980..3717
                     /locus_tag="SCK51_002239"
     CDS             2980..3717
                     /locus_tag="SCK51_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEG1252827.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3872..4840)
                     /locus_tag="SCK51_002240"
     CDS             complement(3872..4840)
                     /locus_tag="SCK51_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEG1252828.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5072..5905
                     /locus_tag="SCK51_002241"
     CDS             5072..5905
                     /locus_tag="SCK51_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEG1252829.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5939..6778
                     /locus_tag="SCK51_002242"
     CDS             5939..6778
                     /locus_tag="SCK51_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1252830.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6804..7364
                     /locus_tag="SCK51_002243"
     CDS             6804..7364
                     /locus_tag="SCK51_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1252831.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7563..9284
                     /locus_tag="SCK51_002244"
     CDS             7563..9284
                     /locus_tag="SCK51_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEG1252832.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9449..10591
                     /locus_tag="SCK51_002245"
     CDS             9449..10591
                     /locus_tag="SCK51_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG1252833.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10607..11818
                     /gene="thiI"
                     /locus_tag="SCK51_002246"
     CDS             10607..11818
                     /gene="thiI"
                     /locus_tag="SCK51_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEG1252834.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4152 a         2042 c         2530 g         3456 t
ORIGIN      
        1 ggagtaaata aagcttatgc aaagaaaaag aaatccaatc acttgggttt ctttttcttt
       61 tattattatg atgaatcaag ctttctgttt ttgtcacgta tgttgtaatc tttacgaaag
      121 agtagagttg gctactcata atcaaccata cttcccatca tcgctaaata cgaaataaat
      181 cagcattcct acaccaatca aagcacaagc tgcaccaaga aaagggatga cattcccaaa
      241 gttatctgta ttcataagta tgccaccacc cacaaacaaa aagccgccag caatcaggtt
      301 tttcatttta ttcatctcct ttcttacgtt atagtatttc cttctttcaa tgagtatttt
      361 gaaagctatc ttattgtatg atgttcttct aggcctcttc tttattatac aggccatttt
      421 cgttatctca tagaaatatt cctttttatc tgaaaactat tgtataccgt atgacatttt
      481 tcttcatgaa gttgtaagta tattctcatt caaatgaaaa tttcatcata gaagagattg
      541 tatttctaaa ttcttaagag taaaatacga agtataactt ggatttggaa ggatgattgg
      601 atgaaagcta ttacgcctga tatgacaaca aaatttaaca aacagtttca agcaaatatc
      661 aaacagcagg cattgcagcg ggcagttgtg aagaacggaa ttactgcttc tgctgaaaat
      721 gtttcagcac atgtggagaa tacaccggta ttttctatag atttggctac ggggaaagtg
      781 gctaatcaaa tgcaatcggg gcgttgctgg atgtttgctg cactgaacac attccgtcat
      841 aagatgctga atcaattcca tttaaaagag tttgaattat cacaaaatta cacttttttc
      901 tgggataaat atgaaaaggc aaattatttc tatgaaaaca tcattgcaac aggaaaagag
      961 gcaatcgata gccggaaagt agctttctta ctcgctactc ctcaacaaga tggcggccaa
     1021 tgggatatga tcgtttcatt attccaaaaa tatggcgttg tgcctaaatc ggtgatgccg
     1081 gaaagtgcga atagttcaaa ttctcgagat ttgaataact acttgaacaa attactgcgt
     1141 aaagatgcag tagtgttacg gaaaatggtt gctcaaggag aaacattaga cgctattgaa
     1201 gaaaagaaag aagaaatgct agaaagcatt tataacttct tagctatctc attaggaaca
     1261 cctccaaaag aattcgattt tgagtaccgc gatgaagaaa agaactatca tcttgaccga
     1321 ggactgacac cacaaatttt ttatgataaa tacatcggtg taaaattaga tgattatgtt
     1381 agcgtgatca atgcaccaac aaaagacaaa ccattcaatc gttcttatac agtagagatg
     1441 ctaggaaatg tcgttggcgg gaaagaagtc aaatatctga atgttgatat ggaaactttc
     1501 aaaaaattag cgattgctca gcttgaagaa ggagaatctg tttggtttgg ttgtgatgta
     1561 ggtcaatctt ctactagaac tagtggcatc atggccttag atgcttattc aatggatgat
     1621 ctatttgata cggattttac aatgactaaa gctgaacgtt tagattttgg agaaagtctg
     1681 atgacccatg cgatggtatt gaccggtgtt gacttagtag acgggcaatc aacaaaatgg
     1741 aaagtcgaaa atagctgggg cgaaaaagtt ggaaagaatg gcttctttgt tatgagtgat
     1801 gcctggatgg acgaatatac gtaccaaatc gttgtaagaa aagatttatt gacgaaagag
     1861 caattaaatg cgttcaatga agaaccaatt gttctatctc cgtgggatcc aatgggtgct
     1921 ttagcataga cgagtatttc aatgaagagt atgtagtaaa gataaaaaga gctggcttaa
     1981 gccagctctt tttatcttta aagtccattc cctgtttttt atggtccctg tgctataatt
     2041 ctaaataaag gataaggatt ggaaataatc cttttatcat taaaaatatg atgacgaagt
     2101 agaataaaat tgggagtagg ggggaaagtc atggatatcc aaactgtttt atctgatctg
     2161 aaattaaatg gtaaagtgat tccagttgtt ggtggcgatg ttaatcagac ctatcgtata
     2221 aaaaccgaac atcgtgctta ttttttaaaa atccatccga atgtaaaaaa aggatttttt
     2281 gaagcagaag tagacggatt aaaagaactg tcagcattcg tacgtgtacc ggatacgtat
     2341 atgctaggtg aaacaagtga aggcgcttat ttgttaatgg aatggatcga gccgggaaaa
     2401 ggggatcaac gagatcttgc tgcagcttta gctaatctcc atcagcaaac agcgccacaa
     2461 tttggttttc gtaaagataa ctatttgggt acacttgttc agaaaaattc ttttgaagaa
     2521 gattggtgga cttttttctt taaagatcgg cttgaatccc agatttcttt ggcagaagaa
     2581 acgaatcgat ggaatgtaca gcgtcaagaa aagtatctgc gatttaaaga acgtgtattg
     2641 aaatcggttg aaccaaaaaa gatcactcca cgcctcttgc acggagacct gtggagtggt
     2701 aatgtgttct ttgaccagca agggcatcca gtttttgtcg acccagctgt ttcttatggg
     2761 aatcgggaac aagatattgc aatgagccag ttattcggtg gtttccgacc ggaattttta
     2821 gatgcttatc aaactatatt tccattagaa aagggatgga aagaccgact gccgatttac
     2881 cagttatatt atcttttggc acatttgaat atgtttggag aaagttacgg ttcccaagta
     2941 gatcaattac tagaaaattt ttaagaggag ggcgtgtttt tggcaaaaaa atataccaaa
     3001 aagcatgaag tttcatatta tgagtgtgac atcaaccaaa cgatgacatt tccttcgatg
     3061 ttaggagtag ttatcaaaac gtctgaagat caaagcgacc agttgggaag aggcagtgaa
     3121 ttcgtcaata gctttggact tacttgggtc atcacgaatt attccatgga gattaatcgt
     3181 ctccctagag tcggtgaagt aatctcagta acgactcaag ccatggagta caataagttt
     3241 ttctgttatc ggaacttctg gatccatgat gaagatggga aagaattagt caaaatagaa
     3301 tctgttttcg tattgatgga ttttgtgaat cgaaaaatga gcagtgttaa tgaagagatc
     3361 attgctcctt ttgagagtga gaagatcaag aaaatcaagc gtcaggaaaa aattgaaaag
     3421 attgattcgg gcttcatgtt gccatatcgt gttcgttttt atgatatcga tagtaatcag
     3481 catgttaata atgcgatgta ttttaattgg atcattgacg tattaggata tgactttttg
     3541 actacgcatg ttccagaaaa agtaattatt cgatttgata aagaagtaga gtacgggaat
     3601 gagatcgaaa gccattttga acaaatccct atcgaaaatg gtgtcaaaac aaaacatgaa
     3661 atccgaatgc aagaacagct gtattgtgaa gcaaatatta tatggaaaaa taaataaaaa
     3721 agaggttgtg acagaagggg gcaacttcaa gaaataagaa gaaatttacg aaaattgttt
     3781 ctcaaatttt tgtaaatttt cgacttagtt ttcgaagaag ctatttttgt cacagcctct
     3841 ctttttaagt ttagctatta gataatgatg tttagttttg gataaacgaa atcaattctt
     3901 cacccgacaa accatagttt acagcaatca atagttttaa acgtgctttt tgactattaa
     3961 tactgttaca gaaaataatc cccatttttt ctagttcttt tcccccgcct tcataatcat
     4021 aaacaggctc tgcgattcca ttgaaacaac gagaaacaag tactacaggg attttttgga
     4081 caagaagctt ttccaaagca gctaaggctt gtggagggag attccccgca cctaaagctt
     4141 cgattaccaa accgtctact ttcgtatggg cgatggcttc caataagtcc ccttgcatcc
     4201 cagcatatgc tttaacgatc gggatagtcc catctacagc ggaaatgtcc agtcgctttg
     4261 tttctaaaag ttcctgcaag aataaaattc gattcttagt tactaagccg atgggaccca
     4321 atgtaggtgt tcggaaagta gcaacatttg tggtatgggt ctttgtcacg tagcgtgcag
     4381 aatggatctc atcgttcatg accaccaaaa cccccttatc tagcgcttct tcacaacttg
     4441 cgacacgaat cgcactttca aaattataaa ggccgtcact tccaagttca ttacttgaac
     4501 gcattgcccc agtcaaaaca attggtaatt tgttgccaat cgttgtatca agaaaaaaag
     4561 cagtttcttc taatgtatct gtaccgtgtg tgatcaccac tccggatata cctgatgcat
     4621 aagcggacaa gatacgttct tttaatacca acatgtgaga aggcatgatg tgtggtgagg
     4681 gaagattgaa gatatcttca actaataatt ctacatgctt ggggattgtg atttctgcat
     4741 ctaataatgg gtttgccaca tcaggactaa caccgcccgt gagcatgttt tctttcatgg
     4801 cgatcgttcc gccggtgtgt agtacaagta tttttttcat tttacagact tccttacgaa
     4861 taattttgca tagccgttaa atcttattgt atactatata aaagagtttt gctatcagtg
     4921 aagaataacg aatagcctaa cttatcataa aaaagctgac cttattaaaa tggaaaagac
     4981 tcaaccttga cctcctaata caatggagta tctggttgag tttcttttac aagaaagcag
     5041 ccaaggaaat caatcacgaa ggtggccgga aatgatcaga atcggattaa catcgtttaa
     5101 agaacatgac aaattaactg gtaaaaagaa taacactctt tatgaatatg cagggtttct
     5161 acccttagtg gaattagata ctgcctatta tggaattccc cgaaagtcct cagttgagaa
     5221 ttggataagt gaagtacctg aatcatttcg ctttgtagtc aaagtctatc aaggaatcag
     5281 cggtcaagga gactggaagc cttattatca gaccgaagaa gagatgattg cgcatttttt
     5341 agatgttatg tcgccaatgg tagatgctgg aaagatgttt tgctttttag tccaattccc
     5401 ctcatcattc aaatgtacga aagaaaatgt ccagtacttg cgcaaactta gaaagtggtt
     5461 tgagggttat cctgtttcta tagaattgcg ggattattct tggtacagcg atgaatttct
     5521 tgaacgtaca agaaacttta tgtctagagc cgctttttct ttagccatca tcgatgagcc
     5581 acagttgctg aatacaacga ttccttttga tccatttgtc accaatccga actttgctct
     5641 ttttcgattc catggtagga atgcgcaagg atggcaagca aatgataaag agtggcgtaa
     5701 aaaaagaacg ttatatcgtt attcagaaaa agaactgcaa gaactagcac atgaagtcaa
     5761 agtagttgat tctaagacaa aagaggtagg tgtgattttt aataataatt ctggtggtga
     5821 tgcggctgaa aatgcattag cactgcaaaa aatattaaat atcaagccag atggactcaa
     5881 tccaacccaa atggatctgt tttaagggct tattttattc aagaaagggg cgaagtctat
     5941 gatcaaagct attttttttg atattgacgg aacattggtc accaatcgtt caaaagcgtt
     6001 ggaatcaaca aaacaagcaa ttgaatatgc tcggaaaaac ggcattttat gcggtgtagc
     6061 aacggggcgc agcccagtaa agatcaaaga aatcattgat gaattggaat tagatatgta
     6121 tgtcgtgtat aatggtcaac tggtttttac tgcagatcgc acgattatcg accacccttt
     6181 tgaacaaaaa gtattggaac atattgttga atttgcagat gagaaccatc gacaaatcgt
     6241 ttttggtgcg agaaatcggt tagatggatc aacgacgatg ctattaggac aatcgatttt
     6301 tatcaagcga ctagtcagct ttttaccgcg taaattcccg gtacgtctga tgaaaaaaat
     6361 cctccaggtt tttagccctc accgacaaaa ggatcgttat gaaacgttag agatattaaa
     6421 agaaccaatt tatcaatgta tattgctcag tccggagtca gaacagaaaa agttagaaca
     6481 tcgctttccc gagtgtacgt ttcagcgttc aaatacgtat actgtagata tcatcccaaa
     6541 aggtggttca aaattactag gcatccaagc tttcgcaaat gctgtcgaaa ttgaaatgga
     6601 agaaattatg gcattcggcg atcattacaa tgatatcgag atgctgaaag gtgtaggaat
     6661 tggagtggcc atggggaatg ctcagatcga agtaaaacat gctgcagact ttgtgactca
     6721 atcaaatgaa gaagatggga tatacgaagg actgaaacac ttcggtctga tttattagtc
     6781 aatgaaagaa ggaaaaaatc agtatggaag cagagacacc ttttcaaaaa gtagaaacat
     6841 tccacaaaac ttttgatcca agaagaccag agatacccac ggcttttaca gaaaatgaag
     6901 ctcatacacg tgctgcgttc aaaatagaag aacttgttga atttgttgca gctacttcac
     6961 ctaataaaga acaactgcat gaagctgtta tgctgctgca cgaagcatta gataaagcag
     7021 aagaaaaaat acttggtaaa acacagtggg gaagtgatcc gctggttgaa caagtagatg
     7081 cactgacaga tatcctttac tttacttatg gttctttttc attactagga atagtgcctg
     7141 atcgaatatt cgaaatcgtc catcaggcaa atatggggaa attatttcca gatggaaaac
     7201 ctcattatga ccctgtaaca aataaagtgt tgaaacctga aaactggcag atggaatatg
     7261 ctccagaacc taaaatcgcg gcagaaatcc aacgtcagat tcaagaagcg attgctaaga
     7321 agaaatccgc tgatataggc atgtgcacaa atgaaaaaga gtgatcagag tttcactatt
     7381 tttgttatct ttatacgatg gaaaaacaaa agattttcgt ttaagttagt gaatgcatga
     7441 aaatagtatg ctataatcta gaatagatgt cttttgcacg agagacgtat tatttggatt
     7501 tatgccaagc agaacgccat ctgttgcata aaagatgcat actgaaaacg gaggaaccat
     7561 aaatgggaaa taatgttatc atcggaatcg tcattgcaat cattatagtc gccattgtcg
     7621 tctttttggt tagttacttg atgaagaaaa aaaaccaaga tcgattggat gccttagaaa
     7681 aacgcaagga agaattattt gacttgccag tgattgaaga agtcgatgat gttaaaaaaa
     7741 tgcatctggt tgggcaaagc caaaatacct ttcgtgaatg gagtcaaaaa tggaatgaca
     7801 tatctacttc ctcgttcgct gaactagaaa gtcaaatttt cgaagtggaa aacctcaatg
     7861 aaagttttcg gttcttaaaa ggaaaaaaag cagttgttga agcagaagct acaatggacc
     7921 aaatggagaa acaagtgacg gaaatccgtg aaggtctaaa ggaattgcgg gaaacggaag
     7981 aacgcaactc actagctgtt cagcaggcac ttgacgtcta tgaagaactg aaagaaaaag
     8041 tcagagaaga cgagcatgaa tacggaccag ctctttttga attgcaaaaa cagatcaaag
     8101 tgatcgaaaa agaatttacg cagtttgtga ctttaaacac ttctggagac cctgttgaag
     8161 cccgcgaggt tttggataaa gcagaaaaac atacgtatga agtggaagct ctgatgaaaa
     8221 aaattccacc gctgtatgag gagctccata ccactttccc tgaacaactt gaagaaatca
     8281 gtgatactta cgagaaattg atggaagaac aatatgtatt cccagaagaa gatcttgctg
     8341 aagatatcgc aaaagtaagt cgaagaattg aaaattcatt agccaatctt gaaaaaacag
     8401 aagttgaaac ggtcgaattt gaaaaccgtg aaacagctga tttgattgat tcgttatatg
     8461 atatcttaga acgggaaatg gaagctcaga gatatgtaaa aactaaccaa tcaacgattg
     8521 cagagtatat caaacataca acaaagaata accgtcaatt attgattgag ctggatcaca
     8581 catctcaaag ctatacattg aaccacaatg aaattggccg tgtacgaggt ttccaaaccg
     8641 aagtggaaga aatggaacgt caaaatgaac agatgattcc acagatccgt caacacgaaa
     8701 ttccttattc agaagtgcgc acgttctata aaacagtctt caaagtctta gaagatatcg
     8761 aaaagcaaca agtagagatc gacgactcat tacatgaatt gagaaaaggc gaaaaagaag
     8821 cgcaagaaaa gatcgataca ttcgaattca aattgcgcag tttgaaacgt tttgtagaaa
     8881 aacaacgttt gcctggcttg ccgaatgatt acctagaatt cttttttgtt gctactgacc
     8941 gtattgaaga actaagtgtt gtcttgaata aaattcgagt aaacatggaa gaggtcaatc
     9001 gtttagttgc tctatgtgaa gaagatctag aattattaga taagaagaca catgatttag
     9061 tagatgcagc agcattaacg gagcaaatgc tccaatatgc aaatcgttat cgtcatacac
     9121 acgaagaagt gagagaagca attgataaaa gctactattt attcaataaa gaatatcact
     9181 atcaagaagc attagacgaa atcggtacag cattagagcg agtagaacct ggtgctttca
     9241 aacgcatcga agatttttat tttaatcatc cagacttagt ataaagcaaa aaagttagta
     9301 aaccagcaag agcaagacgg attattgtct tgttcttgtc tttttttgta tatttgaaaa
     9361 ttgaaacaag tgaaaatttc acctgctctt gaaagcacct tggacaaccg ttataataga
     9421 aaagaatttc aaaaaagagg ggtttcttat gatatatttt gataacagtg caacaacccc
     9481 aatctttccg caaagcttag atgcatatgt aaaaaccagt cagcggatta ttgggaatcc
     9541 atcaagcttg catgatttag gcaatcaagc taaccggttg ttgcagcaag caagaaagca
     9601 gatcgctgat ttgatccatg tagaaccaga agaaatctat tttacttcag gcggaacaga
     9661 aggagataac tgggtattaa aaggaacgat gatcgaaaaa agagaatatg ggaatcatat
     9721 gatcatttca ggagtcgaac atccggccgt cagcgagaca gcagaacaat taaaagaatt
     9781 aggaatcgaa gtaagtatcg ccccagtgga taaacgcgga tttgtacgtg tcgacgaatt
     9841 gaaagaatta ataagaaaag agacagtatt agtctctgtt atggctgtga ataatgaagt
     9901 aggatcgatt cagcccatcc aggaaatcag tgatcttttg gatgattttc ctaaaattca
     9961 tttccatgta gatgctgttc aggcgattgg aaaagtacca ttctcgcaat ggttgacaga
    10021 aagagttgat tttgcaacat tttcagctca taaattccat ggcccacggg gaactggttt
    10081 catttattgg aaaaagggtc gtcgtttagc accattactg acaggtggag ggcaagaaag
    10141 aaatcagcga agtgggacag aaaatgttcc aggtattgtg gctatggtta aagctttacg
    10201 tttatcatta gagataaagg aacaaaaacc tgagcagaca caacgcttaa aagtagctct
    10261 aatcaatgcg ttgaagacgt atgagaaagt gacgattttt tcagaaggaa ctgactatgc
    10321 accgcatatt ttatgttttg ctctaaaagg aattcgcggc gaagttctcg tccatgcctt
    10381 agaagaaaaa caaatatttg tttctactac tagtgcctgc tcctcacgta aacatatggc
    10441 aagcagtaca ttgcacgcaa tgcatgtacc aaatgatctg gctacatctg ctgttcgaat
    10501 cagcttagat gaaaacaatt cgcttgcaga agtcgagcaa tttatgatta ttttcaagca
    10561 gctttataag aaattttctg caatcaatta aaagaggagt gttcttgtga aatacagtga
    10621 aattatggtc cgttatgggg aactttcgac aaaaggaaag aatcgaaaaa gctttatcat
    10681 gcaactagca caaaatgtca gacaagcttt gaaagacttt ccagaaatca aaatccatgc
    10741 tgatcgggac cggatgcatc ttttattgaa tggcgcagac gggcagctag ttattccaaa
    10801 actagcaaaa atatttggga tccaaaattt ctctccaagt attcgtgtag aaaaagatgt
    10861 cgaagttttg aaacaagctg ttcaagacat catgaaagaa atttatactg ggaaagaaac
    10921 atttaaaatt atcgctaaaa gaagcgatca ccaatttgaa ttgacaagta atgaattgaa
    10981 tcaaacatta ggtaatgctg tttttgatat tttcccgcat atccaagttc aaatgcgtca
    11041 accagatatt ccattacgtg tagagattcg gcgtgatggc gcttatctat cgtatgaaac
    11101 gatcaaggga gctggaggat tgcccgtagg aacaagcgga aaaggaatgc ttatgctttc
    11161 tggggggatt gattctcctg tagcagggta ctttgccatg aagcggggag tggaaattga
    11221 agctgttcat tttgctagtc cgccgtatac aagtgaacaa gcactgcaaa aagcaaaaga
    11281 tctaacagcc aaactagcac cttatgtggg aacgatccat tttatcgaag tgccatttac
    11341 cgagattcaa gaagaaatca aaaagtcagt gccacaagga tattggatga caattactcg
    11401 tcgaatgatg ttgcgtttga ccgatgaaat tcgccgtatt cgtcatggtt tagtcatttt
    11461 gaatggtgaa tcattaggtc aagtggcttc acaaactttg caaagtatgg tggctatcaa
    11521 tgaagtaacg aatacgccaa tcattcggcc tgtagcgaca atggataaac ttgaaatcat
    11581 tgaagtagca gaacagatcg atacatttaa tctttctatc cagccttttg aagattgctg
    11641 tacgattttt gctcctccac agccaaaaac acgccctcaa ctggaaaagg tccatcaata
    11701 tgaagagcgt ttagcaatag atgaaatgat ccaacgagca ctagctggct tgaagatgga
    11761 agagatacaa gcagaatcgt ctaatgaaaa aactgaagaa ttttcagaat tgctataaga
    11821 ggttatgaaa atagtgtttt gaccagagta ttagagaaac aaaacgaaaa aatagctcca
    11881 tgtattttac cgtttttgaa ctaatgtcga agattagctg attgaatacc atttatcaag
    11941 aagttatgga aaagccggtt cttttttact aactgaaaaa taaaatttta tttagagatc
    12001 aagctacagt tgataaatga tataaaaaag agaatttccc atgaaatagc gaggttgtga
    12061 cagaagtggg cagcttcaag aaataagaag gaatttacga aaattgcttt tcaaattttt
    12121 gtgaaatttc ggcttatttt tcgaagaagt tacttctgga acaccgtgta tcagaggttg
//
LOCUS       DARBFO010000078        11802 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000078 DARBFO010000000
VERSION     DARBFO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.079"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1696)
                     /locus_tag="SCK51_002247"
     CDS             complement(<1..1696)
                     /locus_tag="SCK51_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEG1252835.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKK"
     gene            1819..2325
                     /locus_tag="SCK51_002248"
     CDS             1819..2325
                     /locus_tag="SCK51_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEG1252836.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            2404..3126
                     /locus_tag="SCK51_002249"
     CDS             2404..3126
                     /locus_tag="SCK51_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1252837.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(3204..4604)
                     /locus_tag="SCK51_002250"
     CDS             complement(3204..4604)
                     /locus_tag="SCK51_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEG1252838.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(4774..5748)
                     /locus_tag="SCK51_002251"
     CDS             complement(4774..5748)
                     /locus_tag="SCK51_002251"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEG1252839.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            6101..6661
                     /locus_tag="SCK51_002252"
     CDS             6101..6661
                     /locus_tag="SCK51_002252"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEG1252840.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            6678..10199
                     /gene="mfd"
                     /locus_tag="SCK51_002253"
     CDS             6678..10199
                     /gene="mfd"
                     /locus_tag="SCK51_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEG1252841.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            10214..>11802
                     /locus_tag="SCK51_002254"
     CDS             10214..>11802
                     /locus_tag="SCK51_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1252842.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKI"
BASE COUNT         3541 a         2190 c         2551 g         3520 t
ORIGIN      
        1 gtttcttcgc aagatcttcc gtcgtcattg aggcagtatc gcgtagtaaa tggatatatt
       61 cttcttcaaa atttgctccc ttttggttgg cgtaagcata gatgcccaaa ctaaagaggt
      121 agccaaatgt gtatgggaag ttataaaacg gtacgtcatc aataaagaaa tgcaattttg
      181 cagcccagaa atatggatgg taagtagcta aagctccttg atagccttct ttttgcgcct
      241 caaccatcat ctctgtgatc ttatcttctg agactaatcc ttcttgccgt gcttcataga
      301 atcgattttc aaaaatgaat cgggaatgga tattcatgaa catagctaaa gcattttgca
      361 gttttgtatc tagcaaattg attttttctt cttttgtttt tgcgactttt aatgtagcat
      421 cggctacgat cagttctgcg aatgtacttg ctgtttctgc gacattcatg gcatattcgc
      481 gatttaaaga aggtaaatcc cacatcgtgc tgctatggaa agcatgtcct agttcatgag
      541 caagtgtcgc aacctcatta acagaattac tgtaggtcat aaagatcctt gattcttttg
      601 tttctggtaa ctcagtacaa taaccaccag gacgtttccc aggacgatct tctgcttcga
      661 tccagctctt ttcaaaagca gattgggcaa atttagccat ttttggactg aatttgttaa
      721 agttttcgat aataaatgct gctgcttcgt caaaactaaa ggttttttct ttcaaatcgc
      781 ctaaaataat aggtgcatct tgatcttgcc attccatttt ttctttaccg aaaagattag
      841 cttttctagt caagtaatta acaaggggtt gtttgttttt ctggattgta tcccacatga
      901 cagacaatgt ttctttcttc aaccgattgt actctaatgg tttttgtaga aaatctgtga
      961 caccatgaag ttcatagtca gaaagtctaa aaccatctaa atgattcaaa gtatccgtga
     1021 agaggggtgc cttttctttc caggcttctt cccatgctgc gaaaagtgtt tctctgactt
     1081 ttggatctgg atcgcccatc atcttattga aggcttgtcc agcagataat tcgactactt
     1141 gtccatcttg ttcgaaggga atcgaaatgc tggctacaat cgtatcataa tgactgctcc
     1201 aagcatttag accatctaaa gaaagcgtat ttataatgtt ttcttcagct tctgataata
     1261 attgtgagcc atcacgacga atctcgttta aacgaaaagc aattgtttca aacgaagatt
     1321 gagaaagcat ctgattccaa tcattgtcag atatttctgc aaatttcttt gacaatacag
     1381 tttcggctga ttgcagacga ggcagcattg cgtaaagctt gcctgataag acctttgcat
     1441 ctgaatcatt gacattggca gaaagcaatg cagtaatata gctgctgcat tgggtaaaac
     1501 cattcgtaat cgtttcttgc aattgaagaa tagcgtttaa ctgctctgca ttgtcagaac
     1561 taggtgacca ttttgttacg cggtgataat actcgttcat ttggtcttcc aattgcttca
     1621 tccgttggtt caaagcatca gaatgactgc ctccggaaaa aatagaatct agattccaat
     1681 ttaatgaata actcatgaaa aaactccctt catattgtaa tacaaaatta agtatagcac
     1741 ttttttatgg aagaagtctc tatgaaaatg attgttgttt cttcaaaaaa agtctaagat
     1801 aaaatacaga ggtgacttat gaattcttca aaaaaacaaa tgaaaaacgg aaatatttat
     1861 ttagtaatcg cactgatagt cgttataatc ttattttaca gttcttctca aacttacgga
     1921 cagcaatcac aagtttcgcg attagaaagc tggctgccta atcagccatt taaagacaca
     1981 ttagcgggta tacgttttaa ttatggtgga agtatggtga gtatcaacca tctgggctac
     2041 gccaaatttt tagagttctt tttaaggaag ggcgcacatt tcggtaccta ttttattttg
     2101 ggaggcagct tgtttttagg tgttttcccc aagctaaaaa tttggtggct gacagcgatc
     2161 cttgcttggc tttcagcaac agggtacgca ggtttggacg aattccatca gatgatgacc
     2221 ggtggacgaa cacctatgtt ccaagatgtc atgttggatt cgatcggtgc tctgactgct
     2281 gtgatattat gtgtgctttt tgcggcattc aagaagcgtt tatagattgg ctgtggcttt
     2341 gtttttttgt gaaatagtac tagaatagaa gtgtattctc aaaagaacaa gggaggaaaa
     2401 atcatgtcag gacatagtaa atggcataat atccaaggaa gaaaaaatgc ccaagatgca
     2461 aaacgaggga aagtctttca gaaactttct cgtgaaattt atatggcagc taaagcaggc
     2521 ggtcctgatc catctatgaa tccagctttg cggcttgttg tggacaaagc aaaagctgca
     2581 aatatgccta acgacaatgt ggaacgggcg attaaaaagg ctacgagtgc cgtagatgaa
     2641 acgaattacg atgaaatcac gtatgaagga tatggtccgg gtggagtagg aatcttagtc
     2701 catgcattga cagataaccg taatcggaca gcgacaaatg tccgagtagc ttttactcga
     2761 aacggtggat cactgggaga aacgggttca gtcagttact tgtttgaccg caaaggatat
     2821 atcgctattg aacgcgatgg gttgactgtt gatgaagaga cgatgtttga ggatgtatta
     2881 gaggcggggg cagaagatct gcaaacttct cctgaagtat tcgagattta tactgcacca
     2941 gaagattttg ctgctgtacg agatgagttg gaaaaagagt tcactttggc gcaagcagaa
     3001 ttgacgatga ttccacaaac gacgattgat ttaaatgagg aacaaaaaga gcaattggaa
     3061 cgtctagtag ataagctaga agacgatgat gatgtttcag aagtctttac tgctgctgaa
     3121 atgtaagtag cacattgtta gataataaaa agaaagcacc tgtaccaatc gtacgggtgc
     3181 tttcttttta tatattattt ttgttagaag accaatacag gcgttccttt ttcgaccatc
     3241 ccgaataatt ccttcattac acttggtggg gtgttgatgc agccgtgtga accgcgggtt
     3301 ttccataagt ctccaccgta ttcaggttgc cagtctgaat cgtggatacc aacacctgtc
     3361 cagtcaatcg gcatccagta gtttaccgga ctctcgtaag gtgttccatc gtcatttgtt
     3421 ccttttaaag ttgcatcttc ttctttattc caaacataga agactccagc aggtgtaggt
     3481 gtagttggct ttccggaaac gatatctgtt tcaagggcca cttttccgtc tttgtaatac
     3541 cacatatgct ggttctccag atcaacttcg atatacgtat cttcgatcaa tggatgatca
     3601 gcggttgtgc caccttgcac aatgggtgaa cgagtaaaat cttggcctgc cagaatcgct
     3661 tttttcaatg cttctgtctc actgtctgtt tggatcgtcc aactgtaagt gccaactggg
     3721 accgtaactt ctcctctttt tgtactttta aattttgtgt cattcgtact tgtgttatac
     3781 tttgtgccta aatctgttac atattgacgt acttgttctg tatctaaatc gacttttcca
     3841 tcattgtagg tcagccaact cataatatca cttgatggga tctggaaagt ctcaccgttt
     3901 atactgtaag tagcttttac atttgcgata gcattcatgg atgcgagttg ttctttcagt
     3961 gagctgtctt cagatgttac ttttggtttt tctttgaatt cagtcaattc aattgtatct
     4021 ttaccagagt ttaccgcact ttttaattgt ttgaccgcag catcgacgtc gatcgtattt
     4081 ccattgactt caggcttgat aacgaaagag tcacctgctt tttcgatcgt tgcatcagta
     4141 gtaggtgtcc ggtctttgtt caaatcagtc agcttagttg tcaatgtctg aacggtagtg
     4201 tctagttttt ggtggttttg actgacagga tctatttctt gtttttctgc cgctgagaca
     4261 taagtcattc cccaagtcca tccgttttgt tcatttagga tcggagaaag ctccgaagtg
     4321 aaatcatctt tgtagccgag ctcggatttt ggtacttcct gccagacttt tccatcttct
     4381 ttaatagcca gttttttatc agaataagct tcttttaatt tttcatttgc tttttcgact
     4441 gttagattgc tgacatttat tccatttatt tctgtattcg gcaaaaaatg ctttgtgtag
     4501 tatgtactgc gataggtata aaagctgatc agcactaaga gaacaccaag aactgaaagc
     4561 aaagcaacat gtttcgattt ttttcgatgc gaagatcttg tcatatcgac aactccttaa
     4621 gtaaaagttt agggtggaac ggtctcatga tagcataatt tagaggaaaa gacgaaggtt
     4681 atctctactt tatgaaaaaa tttagaaacc ataagggtga taaaataaaa aaacggaaaa
     4741 caccgaatag atgttttccg tcatgttcaa agattattca gcgtctagtt tagcaaaagc
     4801 ttcatctaga atttctttta attgcttagc agaagcagcc atacggtctt gctcgctgtc
     4861 acttaaagga atttcgatta ctttttggat accttgacgg ttgataacag caggtgcacc
     4921 aatgtagatg tcgttcaatc catattcgcc attcatgaat actgataaag gaagaacagc
     4981 attttcatca tctaagatgg cacgagtgat acgtgctaat gctactgcga taccatagaa
     5041 agtagcgcct tttttctcga taattgtgta agcagcgtca cgtacgccaa agaataggtt
     5101 aaccatagct tcttcatcga tttctgggtt gttttttacc cattcataga tttgcaagcc
     5161 ggcaacattt gcatgtgacc aaactgggaa ttctgaatct ccgtgttcac ctaagatgta
     5221 agcatggacg ttacgtgcat caacgtcaac taattccgca agtgcttgac ggaaacgagc
     5281 tgagtcaaga gaggtacctg aaccgattac gcgttcttta gggaaacctg agaatttcca
     5341 agttgagtaa gtcaaaatat ctactgggtt agcggcaact aaaaagatac cgttaaatcc
     5401 tgattcaacg atttgtgtga caacatcgcg gttgattttt aggtttttgt ttactaagtc
     5461 taaacgagtt tcacctggtt tttgtggtgc gccagctgta ataacaacta agtcagcatc
     5521 atgtgcgtct gcgtaagtag cagaatagat ttttttaggt gaagtaaatg ccaaagcgtg
     5581 agacaagtca attgcgtctc cttcagtttt agcagtattg atatccacaa tacctacttc
     5641 ttgagcgata ttttgagtaa ctagagcaaa ggcatagcta gaacctacgg cgccgtctcc
     5701 aacaagaata actttttgat gatctttttt ttctgcagct gcagtcattt agtatcaatc
     5761 cttccattga tttgtttttt cccatgctta tgctaacatt tctcaagccc catgtcacgc
     5821 tatttggcta taaaatataa cagataacaa ggttgtatta tatgtaaaag cttacatgtg
     5881 cgttatttca ctatttttat atatcaatta tttcattaaa ctttgaagaa atttttaatt
     5941 tctttatcat ttattaacga ttctctttta gccgaaacgc cttgttttgt gttatactat
     6001 gcattgacaa gtgtaaaaaa gaaaaaagct cgatggactg gaccgctaat tagcagtcca
     6061 gccttttgtg ctgcttttta gaattaggtg gaaaacaaaa atgaaaatga tcgtcgggtt
     6121 aggaaaccca ggtaccaaat accaatatac aaaacacaat atcggcttta tggtcgtgga
     6181 taaaattgca cgcgagcatc aagctacttt caaaaaaaat ccgttcgaag cagaagttgc
     6241 cgaatttttt cataatggag aaaagatcct tttagtcaaa ccacaaacat tcatgaatga
     6301 atctggtcgt gcagtcggcc cactaatgac ttattttggg atttatccag aggaattagt
     6361 cgtaatctac gatgatttgg atctggcagt gggaaaaatc cggctccgtc aaaagggaag
     6421 tgccggcggg cataacggta tcaaaagtat tatttcccat ttgaatacga atgtctttga
     6481 tcgaatcaag gtgggaatcg gcaggccaga agggaaaaaa acagtagtcc agcatgtgct
     6541 tagtcctttc agcaaagaaa atcagccttt gatagaagaa agcatgtgtc aatctgttaa
     6601 agcagtcgaa tatctcatag aagggcattc gttcgtggat gcgatgaatc gattcaattg
     6661 aggaaggagg aaaaggaatg gatattattc aattagtcgg aaaagatccg caagtaaaag
     6721 aatgggtcag caatttagaa gaaaaaatgc ctagacaact aatcacagga ttagcgggct
     6781 cagcgaaaac acttctattt gctcgagcgt ttaagaaaac aaataaaaac atgattattc
     6841 tgatgccaaa tttgtattat gcgaatcagt tagcagaaga tctgcaacat gtgattccgc
     6901 aagatcagat ccatttattt ccagtggatg aagtgctttc tgctgaaatg gccttttctt
     6961 ctccagaagc gcgaggcgag agagttgcaa cgcttaacgc acttcaagaa aaaaaagcag
     7021 gtatctattt gttgccagtc gcatctttgc acaaacgact gccgaataag aaaacttggc
     7081 tacaggcgca attgaattgg catattggtg atgagataga cgtggaaaat cttcctcgcc
     7141 aattgacttt gatgggctat gtacgagaag agatggtcgc aaaaccggga gaattcagta
     7201 ttcgaggaag cattatcgat atttacccgt taacgacaac ctatcctgtt cgtgtagaac
     7261 tatttgatgt agagatagat tctatgcggt attttgaagc agaaacacaa cgctcaatcg
     7321 aaaaaacgga agaaatctgg gtttccccaa cgagtgaaca agtctacgga ccagaggatc
     7381 tagaacatgg aatagagcaa atgcaaaaaa tgctagaaaa acggcttgct gcgaccacag
     7441 aagaagcaga ccgcgagttt ttgcaagatt acttcggtca gctgacttca agctggcaag
     7501 caggtgtccc gacagaagaa gcaaaattct actcagatct tctttaccaa gaatctgtga
     7561 cgatcttgga ttatttccct tcaaatagtt tactagtcgt tgatgattat cagcgaatca
     7621 tggaaacaaa tcgggaaatc gaaagggaag cggcagaatg gcagactcaa aaaatcagcg
     7681 aattgcgggt gttttctgag cagacatttt cagcagatat acaaaaaatc gtgcagaagg
     7741 aaaagttcac cacaaccttt ttctcactct tccaaaaagg gatgggaaat cttcgtttcc
     7801 aagcaatcca taattttcaa tatcggacga tgcaacagtt cttcggacaa atgccattgc
     7861 tgaaaacaga aatggaccgt tggcgcaagc aaaaacagac tgttgtgatc tttattccga
     7921 caaaggacag aatacgcaaa gcagaacaaa tgcttcgaga tgaagacata ttagtagtag
     7981 aaacagaaaa aaatgcattg atcagaggac aggtacaatt agttgaagga gctttgcagg
     8041 ctggttttga acttcctcaa gaaaaaatag tagctatcac agaaaaagaa attttccaaa
     8101 aaacgacgaa aaaacaaacg agacgtcaaa cagtaacaaa cgcagagcga ttgaaaagct
     8161 ataatgagtt aaaagcagga gattacgttg tccatgccaa tcatggtatc gggaaataca
     8221 ttgggatgga aacgctagaa gtagatggcg ttcaccaaga ctatatgact attttgtatc
     8281 aaaacgacga caaactgttt attcctgtta ctcaactgaa tctgatccaa aaatatgtcg
     8341 cctctgaagc caaagcgcct cgcatcaata agctaggcgg aagtgaatgg acgaaaacca
     8401 agcgaaaagt ctcttcaaaa atcgaggata tcgctgacga tctaatcaaa ttatatgccg
     8461 caagagagtc agaaaaagga tatgcttttg gacctgatga cgcctaccaa aaagaattcg
     8521 aaaatgcctt cccctactct gaaactgatg accagttgag aagtgcagcg gagatcaaac
     8581 gggatatgga aaaagaaaaa ccaatggatc gactactcgt aggtgatgtt ggttatggga
     8641 aaacagaagt agcgcttcga gcggccttta aagcgataaa ggaaagcaaa caagtagcct
     8701 ttttagttcc aacaacgatc ttggcacagc agcactatga aacgatgctg gaaagatttg
     8761 aaggtttccc cgtaaatatc gggctattaa gtcgtttcag aacaagaaag caacaaaaag
     8821 aaaccatcga acaactgcgt acaggtcaag ttgatattgt tgtaggaacc caccgaattt
     8881 tgtcaaaaga tattgaattt agcgatttgg gtctactgat cattgacgaa gaacaacgtt
     8941 ttggtgtaaa acataaggaa cgattgaaac agttacgtgc acaagtggat gttttgactc
     9001 tgactgcaac accgattccg agaactttgc atatgtcgat gttaggcgtg cgggatcttt
     9061 cagtaatcga aacaccgcct gcgaatcgtt atccgatcca aacgtatgtt atggaaaaaa
     9121 atcctggggc tattcgcgaa gcaatccatc gagaaatggg acgaggtgga caagtattct
     9181 atttatataa tcgggtagaa acgatcgaac aaaaagtaga agaaatccaa gaactcgttc
     9241 cggaagcacg gatcggttat gctcatgggc aaatgacaga ggcgcaatta gaaaatacgc
     9301 tttttgaatt tattgaaggt caatatgatg ttcttgtcac tacaaccatc attgaaacag
     9361 gagtagatat cccgaacgca aatacgttgt tcgttgagaa cgctgattat atgggattat
     9421 ccacgttata ccaattgcga gggcgggtag gaagaagcag ccgtgtcgca tatgcttact
     9481 ttatgtatga gcagcagaag atccttaacg aagtcagtga aaaacgtctt caggcaatca
     9541 aagattttac agaactggga tctggattca agattgcgat gcgggatctt tccattcgcg
     9601 gtgcaggtaa tctcttaggt gctcagcagc atggatttat tgatgcagtc ggtttcgata
     9661 tgtattctca aatgctaagc gaagctgttt ctcgtaaaca aggaaagaat agtcaagtgg
     9721 agaagaccac tgttgagatc gacctcggtg tcgatgcgta tctacctgaa acatacgtgg
     9781 cagatcaacg gcagaaaatc gagatctata aacgaattcg tgaacttgat tcgcaagaaa
     9841 tgctagatga actagaagat gatctgctcg accgttttgg agaatatcca gaagaagtag
     9901 cacatctctt gacaattggg cagatcaaaa tggatggcga tcgagcgctc ctagaaacga
     9961 taaggaaaca ggaggcgcat atagtcttta ctctaagtaa agttggaaca aaacgctata
    10021 gtgtggaaca gttattcgaa gcactgacag cgacaaaact aaaagccagt ctaggcgttg
    10081 aaaaagaaca gatggtgata cgcttgacga tccccaacca aatggaagaa gcagtatggc
    10141 ttcaggaaat ccaaaagttt gtcaaagcgt tgcgtgagca aaaatatctg tcagcgtagg
    10201 aaggagcaat gatttggatc ataaagaaat gcggcgcgtc atgcaaggag catttgtatt
    10261 gacgattgct tctttcatag caaaagtatt aagtgccctt taccgaattc ccctccaaaa
    10321 cttagtcggc gacgaaggtt tttatgttta tcagcaagtt tatccaattt atgggatagc
    10381 aatgacttta gctttatccg gtcttccgca gttcatatca aagtacgttg cagaaagaaa
    10441 gaatcctttc gagcgaaagc aagctttgca aaatctttat cccttagtat tttggacagg
    10501 tgtctttttg tggttgtttg tctttgcatt tagcagctgg attgcttgga tgatgggaga
    10561 catacaattg aaaccgctga tccaagtagt ttcctttacc tttttgctga tgcctggtct
    10621 atctttttat cgagggaatt tccaaggaag attcttgatg gaaccaactg ctgtttctca
    10681 agtagttgag cagttcgttc gtgtttttgt gattgtctgt agtgcgtttg cttttagaaa
    10741 gcttggatgg acggtgtacc aaacagggac ggcagcaatg agtggagctg tttttggcgg
    10801 tttatgcgca tatgggatcc ttcactacta tgaacaaaag attcatggag gagcgttaag
    10861 cttccgacgg tttccattgt tcaaacagcc gtcaaagccc ttgatccggc gttttctggt
    10921 agaaggcgga ctggtttcga tttatagtgg attgcttatc tttttccagc tgatcgactc
    10981 tttctttgta aaaaatgctt tggaagttta cggactgtca gaatacggtg cgaagatagc
    11041 aaaaggggtc tatgatcgag gtcagccttt agtacaatta ggtttagtga ttgccactgc
    11101 attaagtgcg acattcctgc ctgcgttgac tcgtcatttg accaaccgga tctatcgtca
    11161 gtttttacag acagccaaaa tctatcttag attgacgacc gctttagcat tagctgcgtc
    11221 tttgggatta gccttgctgc tcccttatat caattatgcg ttgtttaaag attatgcggg
    11281 aaatgcagcg cttgtattat ttgttttttc cattgctttc actgcagtca tccaagctta
    11341 tcaaagtatt gctcaaagca agaactcgtt tcgcccttct ttaaaaggcg caggatgggg
    11401 actattggta aaaggattga ctacttcttt tctgaccggt ttattaggaa cagcaggtgc
    11461 aagcctgtct acccttttag gattaggtgt gacgctttgg tattttgttc gaattgaaac
    11521 aaaagagcta aatgcttttt ggaaagaacg gcattttgga cggaagttga tgttcagcct
    11581 tctttgtatg acagtagtac tgctgctgta ttatggtgta ttgaatatag tatttggtcc
    11641 tgtaactcat cgttccactg ctttttttgt cagcgtgata ggagttggga tcggggcttc
    11701 tgtgttcatt tatgcagtga ttcgtcttcg tctgtttaca gtacgagaat ggctaatgtt
    11761 gccttttggt aagaaaatcc tgcgattaaa taaaaaaatt aa
//
LOCUS       DARBFO010000079        11705 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000079 DARBFO010000000
VERSION     DARBFO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.080"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(163..648)
                     /locus_tag="SCK51_002255"
     CDS             complement(163..648)
                     /locus_tag="SCK51_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEG1252843.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    738..1083
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1128..1769
                     /locus_tag="SCK51_002256"
     CDS             1128..1769
                     /locus_tag="SCK51_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1252844.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1788..2423
                     /locus_tag="SCK51_002257"
     CDS             1788..2423
                     /locus_tag="SCK51_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1252845.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2441..3256
                     /locus_tag="SCK51_002258"
     CDS             2441..3256
                     /locus_tag="SCK51_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1252846.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3575..5011
                     /locus_tag="SCK51_002259"
     CDS             3575..5011
                     /locus_tag="SCK51_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1252847.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5200..7647
                     /locus_tag="SCK51_002260"
     CDS             5200..7647
                     /locus_tag="SCK51_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEG1252848.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7877..8944
                     /gene="rlmN"
                     /locus_tag="SCK51_002261"
     CDS             7877..8944
                     /gene="rlmN"
                     /locus_tag="SCK51_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEG1252849.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9137..10240)
                     /locus_tag="SCK51_002262"
     CDS             complement(9137..10240)
                     /locus_tag="SCK51_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1252850.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10793..11341
                     /gene="aac(6')"
                     /locus_tag="SCK51_002263"
     CDS             10793..11341
                     /gene="aac(6')"
                     /locus_tag="SCK51_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEG1252851.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3880 a         1970 c         2522 g         3333 t
ORIGIN      
        1 aataaatatg gatttggcta actatggaag aatactcgta ttagttttca gcgttcatac
       61 tagttgttac attgtttgat tggccaaaag aatttctgat atattttgtg ctaaataaag
      121 aaaaagacgc ttgaattggg aaataagaaa actactctat cattacaaat tttcataaac
      181 tggcagatgt tctaagtaaa caagtatctt ttcaaagtcg gaaaaatcca taatcaacgt
      241 agtggtattg atatttggat gaaagcctac ggtatcttga cgatcaatag cagcatcgat
      301 cacgacttgg atttttttct ctttgtcgtg catcaatgct aatggagtca ctgtacctgc
      361 tggaacgcct aggaggtcag gaagctgttc ttcagaaaca aaagataagc gcttttcttt
      421 taattgttca gctagttgtt ttaaatcggc gtgcttttca tcaggcaaga tgacaagata
      481 gatttgtttt ccttttcttg atttcaataa tagatttttt acttgcggac caggtaggga
      541 aaaagggaca tttttgactg acgtaatcgc aggatgatcg actttttgaa aagagatacc
      601 cagttttttt agtagttcat aagcttcttg ttcggttgca cgactcatcg cgcatcaccc
      661 tccttttttt attagtgtat cataaaaaag agttgacaaa tgaaaaataa tcatgtaaat
      721 tacacgtaaa gttaaaacaa atgacatcga aaagaagagt agccgttagg tgtttttaga
      781 gagtctctgg ttggtggaaa gaggcagcag ttaaccgtga aacacatctt ggagtcgatt
      841 ttctgaagcg tatagtagga aaatccgggg agcccgttac agctgcggtc gttgctagat
      901 gaccagataa ggctggcttt tccgtgagga gtcggtaaaa aaaggtggaa ccacgtgatt
      961 aacgtccttt gatcttcgga tcaaaggact ttttgttttc atctgaacgg caacgactgt
     1021 gtgaggtttt ttctgcaagg aaaaaataaa cagaggtgga accacgtata atacgtcctc
     1081 ttggcatttg ccaagaggat ttttttattt taaaggagga aaagaagatg gagtatttga
     1141 ttgaagttat gccgcaatta ttagtaggag cactcacaac attgaaatta tttttattta
     1201 ccttgattgg ctcattacca ttagggattg ttttagcatt aggattgtct ttgcatttta
     1261 agccgctacg ctacttgctt caaacatatg tatgggttat gcggggaaca ccgctgttgt
     1321 tgcaactgat ttttattttt tatgggttgc cgacgattgg gatcgttttt gatcgtttcg
     1381 aagcagcact cattgccttt atcttaaact atgcagctta ttttgcagag atctttcgtg
     1441 gagggataca atcgattcct caaggtcaat atgaagcagc gcaagttttg caattaagtc
     1501 cctttcaaac ggtgacgaaa atcgtcatcc cacaagtgac aaagattgtt ttaccttctg
     1561 tagggaatga agttatcaac ttggtcaaag attcttcctt gatctatata ttaggtttag
     1621 gtgacgtaat gcgtgctggg aaaatcgcga tggaacgcga cgcgacttta ctgcctttgc
     1681 taggcgtggc aatcatctat cttacattga ctggtattct aacaatcata atgaaacaag
     1741 ttgaaggaaa attaaattat taccgctaac tgataggagg aagaaatatg attgaattga
     1801 agaaagtgaa caaagcattt ggtgaaaaaa aaataatcaa agatttgaat ctaatcattc
     1861 cagatgggaa aacattagct atcgtaggtc cttccggtgg cggtaaaacc acgcttttac
     1921 gtatcttggc tggcttagaa agttctgaca gcggagaatt ttatttagac ggtgtaccgt
     1981 tcaacccatt cgagcttaaa atgaaagaac aagttgtagg agtcgtattc caagacttcc
     2041 agctatttcc acatctgaat atttttgaaa acattacctt agcaccaaaa atggtattga
     2101 aacagacaaa agaagaatac accaaaaaag cagaagaatt agcggcttca ttagggatta
     2161 gtgatttatt gaatatctat ccttatcagc tttctggcgg acaaaaacag cgggtagcga
     2221 ttgccagagc attggctatg aatccacggg tattggctta tgatgaacca acaagtgctt
     2281 tagatcctga attacgccag caagtcgagc gtttgatcct atcattgaaa gaagatgggg
     2341 taacccaaat tattgtcacc catgatttag tatttgcaga aaatgtcggt gatcaattac
     2401 taaaagtttc cccaattcat taagaaagga gaagatagag atgaaaaaaa ccagttttct
     2461 attcgctttg atcgcaggtc ttcttctttt tgcaggctgt tcgaacaaaa aaacttcagc
     2521 agataattgg gcgactatca acgaagagaa aaaaatcgtt ataggtttag acgatacatt
     2581 tgttcctatg ggatttagag ataaagacgg gaagctcaca ggatttgata ttgatttagc
     2641 caaagcagtc tttgaacaat atgggattac ggtagacttt cagcccattg actggtcaat
     2701 gaaggaattt gaactgaaca atggaacgat cgatttgata tggaacggct attccaaaac
     2761 gccggcaaga gaaaagaaag tgcaatttac aaaaccttat atggaaaacg atcaagtgct
     2821 gatcacacct aaagaatcag gaatcacaaa ctttggacaa atgaataata agagattagg
     2881 tgcgcaaaat gggtcatctg gttatgatgt atttacgaaa caaccagaag tattaaaaga
     2941 gatagtcaaa aaccaagatg ctgttttgta tgatagtttt aatgaagcat tgatcgatct
     3001 gaaaagcaga cgggtcgatg gtctgctgat ggataaagta tacgcagact attatttgaa
     3061 acaaagaaac gaactagaag acttcaacat aacaaaaggt tcttatgaga gtgaggattt
     3121 tgcagttggt gcacgcaagt cagatagtac attagtgaaa aaaatcaatg aagccctaga
     3181 tcaactagag aaaacaggtg aattccaaaa aatatccgat aaatggtttg gcgaagatgt
     3241 tacgccgaga aattaacaaa taggggactg tgacaaaaga cgtgtcacag tccctttttc
     3301 cgaataaaca gtgttctcct gccaactcct tgtgactcgg agctgaatgg caggctcaca
     3361 acctcttggt aaacggtgtt caaaagccag tttttcggta ttaagccaaa tttatgcaaa
     3421 aatgtagaaa gccattttca caaaattttt cttactactc gaaactaagc gactttttca
     3481 caacctcctt tacataattt tattttgttg tggaaggggt ttcgtgttat tctaaaagag
     3541 gaacgattga aggttatagg aaaggaagag atgaatggaa cacaaagaac ttacctcatt
     3601 tccaaaagat tttctgtggg gctctgcttc tgcagcgtat caagtagaag gagcttggca
     3661 agaagatggg aaaggagaat cagtctggga tcgatttgta cggatacctg gaaagacatt
     3721 taaaggaacg aacggtgatt tggcagtaga ccactatcat cgttataaag aagatattgc
     3781 tttaatgaaa gaacaaggtc tgaaagctta ccgcttctct gtagcttgga cacggatttt
     3841 tcctaatgga cgaggagaag tcaatcaagc tgggctcgct ttttatgaga gattgatcga
     3901 cgaattgatt gaaaacgata ttgaacctat gttgacttta tatcattggg atcttccaca
     3961 agcattgcaa gatgaatata atggttggga atcacgtcag atcattgaag atttcactca
     4021 ttatgcagaa acattatttg aggcattccg cgggaaagtc cattactgga ttagtttgaa
     4081 tgaacaaaat atcttcactt cattaggtta tttgttagca gcacatccgc caggtgtgac
     4141 agacccgaaa cgaatgtatg aagttaacca tattgctaat ttagcaaatg catctgtcat
     4201 caataaattt cacgagttag aaatgcctgg aaaaatcgga ccaagctttg cgtatacgcc
     4261 aaattatcca attgacagta atcctgaaaa tgttctggct gctgaaaatg cagaagacct
     4321 gatggccaat tattggatgg atgtctatat gtggggaaaa tatccgattg ctgccatgag
     4381 gttcttagaa gaaaaaggat gggcaccaac gattgaagct ggcgatgcag aattgctaga
     4441 atctgctaaa ccagattttc ttgggatcaa ttactatcaa acggcgacga atgcattcaa
     4501 tccattaaac ggagttggtg ctggaaaaat gaatacgaca ggaaaaaaag gttcatcaga
     4561 agaaactgga gttcctggaa tgtataaaaa agttgagaat ccatttgtcg aacgaacaaa
     4621 ttgggactgg gagattgacc cagaaggatt aaggatcgga ctgcgtcgta tcacaagccg
     4681 ttaccgcatg ccggtactga ttacggaaaa tggcttagga gagtatgaca agttgacaga
     4741 agataaacaa atccatgacg actatcgaat caattactta caaagccacg tcaaagccat
     4801 caaagaagca atttcggatg gagcagaagt cctaggctat tgcacctggt cttatacaga
     4861 tttgcttagc tggctgaacg gttatcaaaa gcgatatgga tttgtatatg ttgatcaaga
     4921 cgaaacacaa gaaggatctt tggaacgata taagaaagac agttattact ggtaccaaaa
     4981 actgattgaa gaaaatggag agaaccttta gttcacatac ttaaaacaag aaggtacggc
     5041 tggatatggc cgtgcctttt cttgataaaa tcatttagaa attaagaaaa actgtaaaat
     5101 aagctgaaaa atgaaagaaa gcgtcagttc tttcaaacaa aactttagtt cccttacttt
     5161 tttttgtata atgaaacttg atgaaatgag ggataataaa tggcacaaaa gaaacggcct
     5221 actaaaaaaa ggaaaaagac gaaaaaaacg aaaaagcaaa aacagcagca ggaacaaatg
     5281 ctgacgattg gtgttgggtt tgccttgatt ttatttgctg tcttcggctt tttaaaatta
     5341 ggttttttag gaatattgat tgcaaacggc tttcggatca ttgcaggaaa tacttaccaa
     5401 atcctctgtc ttttattagc cgtattagga ttttggattg tcattaagaa tacagagttt
     5461 tcaattggta aaaaccgacg ctggtttggc ggcatactct tttatttcgg tatcttatta
     5521 ttgttacatg cccatttatt tggcaaactc catactgggg aaccaaatat catggggacg
     5581 acatgggatt tgttagccag tgatataaaa caaagtcaag tagacaataa cgttggtggt
     5641 gggatgattg gagcaatcct ttatcatttt acttacttct tgattgcaca gcctggaagt
     5701 tatttagcag cagtgctatt actggcagga ggagcattcc tgttcagtaa tctggaaggc
     5761 tatcagctgt taaatggtat ccaatcaatt ggagaacgag tacaggaatt attggaaggt
     5821 gatcctgcca agcaagctcg aaagcaagcg gcaaaggaag agagaatgaa acagcgcgca
     5881 gaagcgaaag aagcaaggcg cttagctgca aaagaagctg cagaaaaaga agctgcagaa
     5941 tatgagaaga aaaaagcggt gagccagcaa agggaaaata ctgctgatga atggcaggaa
     6001 ccagaaaata cagagccgga gcagctaagt tttgttccaa tcgacagttt tcaagaaaat
     6061 atccatcctg cgaatctaga gaaaccagta ccagatacac caaaacagac caatacagca
     6121 gaaggttttt cagatgagtt gccagaggat gacggtacct cattagaatt tgagattgaa
     6181 gcagaacagg aaaatcaaga ctatgaatta ccgacagttg atttgcttga ttctatacca
     6241 actgtggatc aaagtgatga gtataaaaaa attgaaaaaa atatcggcgt attagaacaa
     6301 actttccaaa gtttcggtgt cgatgccaag gtcgtcaaag ccagcttagg accatccgtc
     6361 acaaaattcg aagtccagcc ggctgtaggg gtaaaagtaa gtaaaattgt gaacttaaca
     6421 gatgatatcg cgcttgcttt agcggcgaaa gatgtccgga tggaagcacc gattccaggt
     6481 aaatcactga ttggtattga agtaccaaat ggaaagatca gtatggtttc ctttagagaa
     6541 atcatcgaag cacaaccaaa ccacccagat aaattactag aagtaccttt aggacgtgat
     6601 gtttctggac gggtacaaac ggctgatctt tcgaagatgc ctcacttact cgtggctggt
     6661 tcgactggta gtgggaagtc agtagcaatt aatgggatta tcacaagtat attaatgcgt
     6721 gctaagcccc atgaagtaaa actgatgatg atcgatccga aaatggttga attgaatatg
     6781 tacaacggca tacctcatct gttgacgccg gtcgtgacga accctagaaa agcagcgcaa
     6841 gccttacaaa aagtagtaca agaaatggaa gaacgatatg aaaaattcgc ggcaaccgga
     6901 gtaagaaata tttctggtta caatgaattt gttcaacaga aaaacttgga aaacggaacg
     6961 aaacatccaa ccttaccatt catcgtagtg atcgtagatg aattagctga tttgatgatg
     7021 gtagccagca atgaagttga agatgcgatt attcgtttag cgcaaatggc tcgggcagcg
     7081 gggattcata tgatcctagc tactcaacgt ccaagtgtgg atgttatcac aggaatcatc
     7141 aaagcaaatg taccttccag aatggcattt gcggtatcaa gcggtacaga ttctcggacg
     7201 atcattgatt ctaatggggc agaaaagctc ttagggcgag gagatatgct attcttgcca
     7261 atgggtgaga ataaaccgat ccgtgtgcaa ggagcattca tttctgacca cgaagtagaa
     7321 cgggtcgttc aatttgttac agatcagcaa gaagctcatt atgaagagaa aatgatgccg
     7381 acagatgaag ttgaaacgac aggtgctcca gaacagccac aagatgagct gtttgaagaa
     7441 gcaaaagcac ttgtcgttga aatgcaaacg gcaagtattt cccttctcca aagaagattt
     7501 cggatcggtt acaaccgagc agcacgttta gtagatgaac tggaagctca tggagttgtt
     7561 ggcccgtcag aaggaagcaa gcctcggaaa gtattcattg agcaggaaga aacatcagaa
     7621 acgttgccgg acgataatga catgtaaaac ttgtgtgatg aagggttgaa aaacgaataa
     7681 ccaataaaaa gacaaggcat gatgatacca aaagaaaaaa tggttttttt ccatcttttc
     7741 taatacggta tgatgtcttg tctttttagt ttttgtgaga ttaaagttct attcgattta
     7801 tttgtgaagt attgcattaa ggaaatgaat catgtatagt atgtagcgaa actttgagaa
     7861 aaagagggaa aatactttgg aaaaacagtc catttatggc ttgacgaatg aagaattaat
     7921 caattggttt atagaaaacg gcgagaaaaa attccgagct gcccaagttt gggaatggtt
     7981 gtatcaaaaa cgtgtaagta atttcacaga aatgacgaat ctatcgaaac aattgatcga
     8041 aaaattatcg gagcatttta tcatcaatcc tctaaaacag atggtcgtcc aagaagcttc
     8101 ggatggtaca gtaaaatatt tgtttgaact ccctgataaa aacatgattg agactgtact
     8161 gatgagacaa gaatatggct tgtcagtttg tgtgacgaca caagtaggat gtaatatcgg
     8221 ctgtacgttt tgtgcaagcg ggctgctcaa aaagaaccgt gatctgactg ctggagaaat
     8281 tgttgcacag atcatgatgg tccaacatta ttttgatgag cgcaatcttg gcgaacgcgt
     8341 ttcacatgtc gttgtaatgg ggatcggtga gccatttgat aattacgaca atgtaatgga
     8401 ctttttgcat atcatcaatg atgccaaagg attggcaatt ggtgctcggc acattacagt
     8461 ttccactagt ggattagcac acaaaatcaa ggaattcgct aataacggct tgcaagtcaa
     8521 tctggctatt tccttgcacg cgccgaataa tgaggtgcga acatcgatca tgcggatcaa
     8581 ccgaagtttc ccaattgaga aattgatgga agctgtggat gagtatttag agaaaacaaa
     8641 ccgtcgaatc acgtttgaat atatcatgtt gaatcaagta aatgatagac cagagcatgc
     8701 gcaacaatta gcagatctat taaaagataa gaaaaaatta gcatatgtca acttgattcc
     8761 atataatcca gtcagtgaac atgatcaata ttctcgcagt ccaaaagctg atgtattgag
     8821 attttatgat gtattgaaga aaaacggcgt aaactgtgtg atacgtaaag aacatggtac
     8881 agatattgat gcagcttgcg gacaattgcg aagcaagcaa atgaaaaaaa cagttgccaa
     8941 ataatcgtat cctactcgtt ttttcaatta ccataaaagg agcagttttc ttaattaagg
     9001 aactgttcaa cttaaataag agaaaagatt ctatagcgaa agctataaag ctttttttga
     9061 caagaaaaac cctcatcaag caaagaaatg tcctcaattt gatgacagcc tgcttgatgg
     9121 gggttttagc aattatttaa ctgattttct tatatccttg ctggctttct tgtgtagcat
     9181 gataagtttc tacgtcaaga atggcctgtt taaatttttc tcgggtgatt tcataaggca
     9241 catgtttaat atcatttgtt gccatggctt tatctaagat tgcttctaaa tcggtcgtga
     9301 cattgagatt catgtcggtt aacgatactg gaagttggtt ctttttcata aacacataga
     9361 ttttatcaaa caaatcgaac tgctgatcca tcatcaataa ggtcagtaca ccataactga
     9421 cagatgctcc gtgaaggtat ccctccattt tttctagaac agtcgtaccg taatagaatg
     9481 agtgtgccat agcagaatta tagttattgt taactaaaac agaaaccaat ccggtagtaa
     9541 caatgatatc cgaaacgact tgttcaattg catatgaggg acgatcgtcg gcactatctt
     9601 tcaatgcttg cgtaccataa gtcaagagac gatcaatgca catttttccg atttggattc
     9661 ctaatctatc cgtgtagcct agatcatcgc cacgagcaga aaaagtagat tcgacttctt
     9721 tggataaagc atcgccaata cctgcccata agtatttatc aggtgcttcg gcaataattg
     9781 ctgtatcaat gaaggtgtgt acaggtggtt ttgaaggaaa gaagagtccg tccattacgc
     9841 cttcttcgtt atagatgaca cataccgcag tgacgggcga acagttcgaa ccaatagtgg
     9901 gaaaggtgaa gactggttta gctagttttt caccaatgac ttttacagta tcgcaagcac
     9961 ggccaccacc gactgcaaac aggacgtctg ctttttggac actttcatgc tggatcaact
    10021 gttcaatatt tccataagtt gaattacccc cgtaccagag actggcgaca acctctattt
    10081 cctgtgtcaa agctactttt attttgtttt ctgcctttga aagtgcagtc ttccctccaa
    10141 tcagcactac ttttgtccca tattctttga cgattccatt tgctttttca tacgcatctg
    10201 ttccaatggt ataatttggt aagaaaacac tttgactcat attcatttcc ttctttcttc
    10261 gttgaaacgg gttaaaattt taaagagtga tcgatctgac tgcggctgta gatggagtag
    10321 aggtgaacct aagacaggat aagtgatgaa taaggtggca tgaacacctt gaaatcatca
    10381 ataagtatcc taaaaacgaa caagtacaaa aacattgaaa acgacaataa tgaatttttg
    10441 ttcctcttag tatttctacc gattcccttt gcttttttgt aattatctaa aaattctaaa
    10501 aattaagatt attatatgaa aacaggtttt atttgtcaat tcataattgg ttttttttaa
    10561 agcaaaagat aatctatatc atgatgaaca aacagagttt tttcctccaa ttgatggctt
    10621 tcctgatctt tcctattttg aataaagtgt ttctgtataa tttttttatt ttataagtaa
    10681 gataaataat gaaagaatag agatactgat tggtagctaa agatttttga gcgagaacat
    10741 ttcgtttttt caaaaggact gttagaataa gcaagaacaa gggggaagac gtatgataat
    10801 cagtgaattt gaccgtaata atccagtatt gaaagatcag ctttctgatt tactgagact
    10861 gacttggccg gaagaatatg gagacagctc ggcagaagaa gtagaagaaa tgatgaatcc
    10921 agaacgaatc gcggtagcag cggtagacca agatgagtta gtaggattta ttggtgcaat
    10981 ccctcaatac ggtatcacag gttgggaatt gcatccatta gttgtagaaa gctcccgacg
    11041 aaagaaccaa ataggtactc gattagtcaa ttacttagaa aaagaagtag cttccagagg
    11101 aggaatcacg atttatttag gtacggatga tttagaccat ggaacaacgt taagtcaaac
    11161 ggacctgtat gagcatacat ttgataaagt ggcttccatc cagaaccttc gtgaacatcc
    11221 gtatgaattc tatgaaaaat taggttataa aatcgtaggt gtcttaccaa atgcaaatgg
    11281 ctgggacaaa ccggatattt ggatggcaaa aacgattatt cctcgaccaa attctcaata
    11341 ataaaaagag gttgtgacag aagtaatcag cttcaagaaa taagaaggaa tttacgaaaa
    11401 ttacttcttg aatttttgtg aaattccaac ttatttccga agaagctact tttggaacac
    11461 cgtgtaccag aggttgtgaa aaagtcgctt agtttcgagt agtaagaaaa attttgtgaa
    11521 aatggctttc tacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca
    11581 ccgtttattc agataaggga ctgtgacaag acttttgtca cagtttcttt ttgtttattc
    11641 ttttttatct ttgtatggca ataatagaca atataacgaa aattatttaa tggcttttgg
    11701 aagac
//
LOCUS       DARBFO010000080        11669 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000080 DARBFO010000000
VERSION     DARBFO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.081"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(37..216)
                     /locus_tag="SCK51_002264"
     CDS             complement(37..216)
                     /locus_tag="SCK51_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252852.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(597..1304)
                     /locus_tag="SCK51_002265"
     CDS             complement(597..1304)
                     /locus_tag="SCK51_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252853.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="SCK51_002266"
     CDS             complement(1297..1734)
                     /locus_tag="SCK51_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252854.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="SCK51_002267"
     CDS             complement(1749..2000)
                     /locus_tag="SCK51_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252855.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="SCK51_002268"
     CDS             complement(2000..2206)
                     /locus_tag="SCK51_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEG1252856.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="SCK51_002269"
     CDS             complement(2643..2903)
                     /locus_tag="SCK51_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252857.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2978..3181
                     /locus_tag="SCK51_002270"
     CDS             2978..3181
                     /locus_tag="SCK51_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEG1252858.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="SCK51_002271"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="SCK51_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG1252859.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="SCK51_002272"
     CDS             complement(3810..4709)
                     /locus_tag="SCK51_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEG1252860.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="SCK51_002273"
     CDS             complement(4722..5315)
                     /locus_tag="SCK51_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEG1252861.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5391..5540)
                     /locus_tag="SCK51_002274"
     CDS             complement(5391..5540)
                     /locus_tag="SCK51_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252862.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5556..7760)
                     /locus_tag="SCK51_002275"
     CDS             complement(5556..7760)
                     /locus_tag="SCK51_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEG1252863.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="SCK51_002276"
     CDS             complement(7771..9231)
                     /locus_tag="SCK51_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEG1252864.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="SCK51_002277"
     CDS             complement(9292..11664)
                     /locus_tag="SCK51_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252865.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2433 c         1958 g         4386 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtccg
//
LOCUS       DARBFO010000081        11229 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000081 DARBFO010000000
VERSION     DARBFO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.082"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            191..1450
                     /gene="lysA"
                     /locus_tag="SCK51_002278"
     CDS             191..1450
                     /gene="lysA"
                     /locus_tag="SCK51_002278"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEG1252866.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1437..2237
                     /gene="dapF"
                     /locus_tag="SCK51_002279"
     CDS             1437..2237
                     /gene="dapF"
                     /locus_tag="SCK51_002279"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEG1252867.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2240..3121
                     /locus_tag="SCK51_002280"
     CDS             2240..3121
                     /locus_tag="SCK51_002280"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEG1252868.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3118..3834
                     /locus_tag="SCK51_002281"
     CDS             3118..3834
                     /locus_tag="SCK51_002281"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEG1252869.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3840..4535
                     /gene="dapD"
                     /locus_tag="SCK51_002282"
     CDS             3840..4535
                     /gene="dapD"
                     /locus_tag="SCK51_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEG1252870.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4551..5552
                     /locus_tag="SCK51_002283"
     CDS             4551..5552
                     /locus_tag="SCK51_002283"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEG1252871.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5549..6910
                     /locus_tag="SCK51_002284"
     CDS             5549..6910
                     /locus_tag="SCK51_002284"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEG1252872.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6870..8021
                     /locus_tag="SCK51_002285"
     CDS             6870..8021
                     /locus_tag="SCK51_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEG1252873.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7996..9165
                     /locus_tag="SCK51_002286"
     CDS             7996..9165
                     /locus_tag="SCK51_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1252874.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9340..10719
                     /locus_tag="SCK51_002287"
     CDS             9340..10719
                     /locus_tag="SCK51_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1252875.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            11000..11229
                     /locus_tag="SCK51_002288"
                     /pseudo
     CDS             11000..11229
                     /locus_tag="SCK51_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3738 a         1956 c         2505 g         3030 t
ORIGIN      
        1 gtataaatag cttctaatag ttcgggtatt ttgtttttat aaccattttt attcttattt
       61 ttaaaaaaat atttcgaaat atactagaca attcagaaaa ttttgtttaa tattattcca
      121 ataaaattca aatctttatc aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag
      181 gaatgaaaaa atgacagaat tgataattgg cggagtcaca gcaagcacat tggcagaaaa
      241 gtataagaca cctctgtatg tgtatgatga agaaaagatg agacaaacga tgctgtcatt
      301 caagcgaggc tttgtatcga aacgttttga tacaaaagtg ctttacgcct ctaaagcatt
      361 ccaaacgatt gagatgctgc atttagttcg tgaatatgga ttcgggctag atgttgtcag
      421 tggtggggaa atctataccg ctttgcaagc tgattttcct agagatacta tctacttcca
      481 tggtaacaac aagacgccag aggaactgat ctatgcactt gaaaatgatt tgttgcatat
      541 tgttgccgat aatttgatgg aagttgaatt attagcagaa ttaagccaac gctatttaaa
      601 aaaaatctgt atcatgcttc ggttgaatgt cggagtagaa gcacatactc atgaatatat
      661 cgtcactgcg catattgatt caaaattcgg catgagctat gaaagtaaag agtgccaaca
      721 agcccttcag ttgataaaag aaagcagcta tctagagtta gaaggatttc atgctcatat
      781 cgggtcacaa atttttgatg ttactgcttg gttagctgaa atcgacaaat tgacgaatta
      841 cttaaccgat tttgaccaac ctctttcctt gaatcttggc ggagggtttg ggattcgtta
      901 tacacaaaat gatcagccga tcccagtcga agaggcacta aaaaaactga ttgaatatac
      961 ggaagaagct ttggaaaaac gaaaactgac cattcgtcaa ttactgatcg aaccgggtag
     1021 aagtattgtt ggagaagctg gtacaacact atacacgatt ggtttcatca aaaagacacc
     1081 acataaaaaa tattactttg tagatggcgg gatgacagat aatatccgtc cagctctgta
     1141 tcaggcagaa tacgattgtg atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt
     1201 gacgattgca ggaaaaatgt gtgaatcagg cgatgtggta attaaagaag cttatttgcc
     1261 gaaagcagaa tcgggtgatc ttttagctgt ttattccacg ggagcttatg gatactcgat
     1321 gagcagcaac tacaatcgag cgacacgacc agcagttgtt tttgtcaata atggccaatc
     1381 aagactgatt gttcgcagac aagcattttc tgatctacta aggggggaag tttcctatga
     1441 tccttcataa atataatggc tgcggaaatg attttatctt attagattac gcagaagaca
     1501 cagattatag tcaattagcc gcttctttat gcacaaaaga aaaatatgat acagacggat
     1561 tgatcgccgt aaaaatcgat ccgttggaaa tgatctatta caacaaagat ggcagccgcg
     1621 cgccgatgtg cggaaatggc attcgctgtt ttgctcgata cgtaaatgat cagaacatga
     1681 tgaatgaaca ggaattcaac gtagagacat tagcaggcac aagaaaaatt acggtgttgg
     1741 atccctcacc tttttattgc agcgtagata tgggagtgcc agatgattct ccggaaaaaa
     1801 tcaaagctaa tcaaacttat ccaataaaaa accaggaaat agagatcaat ggtgaaaaag
     1861 taacgatcac tagtttattt ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc
     1921 gtgaggaagt ctggagaaaa agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa
     1981 aaaccaatgt caactttgta gaagtagtca atgaacaaga attgatcgtc cgtacctatg
     2041 aaagaggagt aggttggaca cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg
     2101 ctaaggaaac tgggaaaatt tcagcagatc aggtagttgt tcacttggaa caaggagact
     2161 tgatgatctc tggtgaggat gcgatacaaa tggctggccc agcagttcat gaatggacaa
     2221 gaatattgga ggaatgaaga tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa
     2281 tgaagaggga tcagtagatt atgctggttt ggaaaaattg atccaattcc atttagatga
     2341 acaaacagat ggattattag ttttaggtac aacaggagaa tcttctacgc tcacacaatc
     2401 tgaggaggag cagatccttc aactgacagt aaagaaagtg gctggccgtg tgccggtgat
     2461 tgcaggagct ggaacgaata atacgaaaga aacgattgaa aaagcgaagc attttgcttc
     2521 tttgggggca gacgcacttt tggtcatcac cccttactac aacaaaacaa gtgatgcagg
     2581 actagctgct cattttacag caattgcaga agcctcaccg attccgctta ttttgtataa
     2641 tgttccttct agaacaggca tgtcgattcc aatccatgta ttagtaaatt tagcagaaca
     2701 tcctaatatc attggtttga aagaagcctc aggagatatg gcttatgtga tggatgccgc
     2761 tcggttgatt ggagaagaat tcttccttta cagtggaaat gatgatttga tcctaccagt
     2821 aatgagtgta ggaggaagcg gtgtaataag tgtatgggcg aatatccaac ctaaaatagt
     2881 ccatgaatta gtaaaagata cgcaggacgg aagatggcag caagcaaaag aaaagcaatt
     2941 aaatgcactt gaactgatcc atgccttgtt cagtgagacc aatcctattc ctgtcaaagc
     3001 agccatgtca ttattagact taccttccgg acctctacgc ctgcctttag tttctctttc
     3061 aaaagagaaa aagaaacaat tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg
     3121 aaaatcggac tgattggcag tggacagatg ggaaatagag tcgaagaagt tgcacaagaa
     3181 agaggagata cggtccttct tttacggacg gcacctaaag aagcaactac gcttgctttt
     3241 acggaactgc cagaactgct tattgatttt tctcatccag ataatttaga gaaaatcctt
     3301 tcgtatagct tatcatatca acttccgtta gtcattggca cgacaggtta tacacaatca
     3361 cagttccaag aaataaaaga acatgccaaa catgtacctg tgatgtatag tgctaatttt
     3421 tcgttcggca tcatggtgat gaatcaacta atcaagcaag cagctgcaat gttgcaaggc
     3481 tggcagatcg aactgattga aaaacatcat agtcgtaaaa aagatgcacc atctggtact
     3541 gcaaatatgc tgcttcaaac aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg
     3601 caagggaaaa aaagagaaga aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta
     3661 cctggtgaac atgatgtatt gtttgctgct actgatgaaa tatttacgat caaacatgaa
     3721 tctttttcta atcgaatctt tgctgcaggc gcagtagaag cagcggactg gttgaaagat
     3781 cagtcaccag gttattacaa attagaagat ttattaatgg acaaaggggt gtaaaaaacg
     3841 tggaagctca agaaatcatc cgctacattc aaacggcatc taaaaagacg cctgtgaaag
     3901 tctatctgaa tgtaaccgag ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa
     3961 gcagcttcgt tgtttttggt gactatgaga cgattgctcc ggttttagaa gctgaatcag
     4021 aaaaaatcat tgaaatcgaa atcgaaactg ctgcaagata cagcgcagtt cctttgctag
     4081 atattaaaaa aattaatgct cgtatcgaac caggtgctat tatcagagat caagtgtcaa
     4141 tcggcaacaa tgccgtgatc atgatgggag cgattatcaa tattggtgca gtaatcggtg
     4201 aaaacacgat gattgatatg ggtgctgtct taggtggacg agcgacagtg ggcaaaaact
     4261 gccatattgg tgcaggagct gtcttagcag gtgtgatcga accagcatcg gccaaaccgg
     4321 ttatcgtaga agacggcgta ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata
     4381 tcggaaaaga tgcagtcgtg gcagcaggag cagtggtttt agaagatgtg gcagcagaaa
     4441 ctgttgtcgg tggaatacct gcacgtgtat taaaaataag tgatgatcag acaaaagaaa
     4501 atacagcatt gattgctgcg ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa
     4561 tagatacctg tattgtgggg gcaaccggat tagtcggcca aacgatgctc aaagtgttag
     4621 aagaatatga ttttccagta ggacgattga aattattagc ttcaaaacgt tcagccggta
     4681 aagtttgtgt attcaagaaa aagaaacaca ctgtacagga attgacagaa acttcatttg
     4741 aaggatatga tttagcgcta ttttctgcag gtgcttctgt atccaaagag tttgcgccaa
     4801 tcgcacgaag tcagggacta tttgtcatag ataattcctc agcatggcgg gaagattcgg
     4861 atattccatt aatcgttcca gaggtcaatc tggaagacta tacattgaat cggctgattg
     4921 caaatccaaa ttgttcaacc attcaagcgg tacttccgct taaagcttta caagaagcct
     4981 ttggtatcca gcgagtcaat tattcgacct atcaagcagt ttctggttcc gggcaaaaag
     5041 gcattgaaga tcttcatcgt acgcaaaatg acgaggaacc agttttgtat ccttataata
     5101 ttagccaaac ggtgattccg gaaatcgatc agcctttgga aaatgggtat acaaaagaag
     5161 aacagaaaat gataaatgag acccgtaaga ttctgcatca gcctgctttg ccagtatcag
     5221 caacctgcgt acgtgtgcca atcgaaaatg gacatggagt ctctgtggca gttcaactgc
     5281 aaaaagagtt tacgacggaa gaaattagag agtgtctcag ccagtttcca gggattcaat
     5341 tagaagatga tctagcagca cataaatatc cgacgagcat tcttgctaga ggaacagata
     5401 tggtttatgt tgggaggatc agaaaagaca cttctttaga gaacggattg ttgttctata
     5461 cgactgccga taacatacgc aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt
     5521 taatgcagaa aatagcggag gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg
     5581 cggaacatcg atgtctcatg aagagcaatt caaaaaagtc cgtacaattg ttctttcaga
     5641 tcccaatcgc caagtgatcg ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa
     5701 agtaacagac ttactttatt tgatttatgc ttaccttcag catcatgttg attggacacc
     5761 cctttggaaa aggatttgtg atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt
     5821 acctattgaa gatgaactag cacgcataca gaaacaactg gaagacgaaa agataactga
     5881 agattattta gtaagtagag gtgaatatct gactgctcaa ctgatggctg agtatcttgg
     5941 ttaccagttt attgacgcaa aagacctgat tttctttgac tatgatggga aaatcgatca
     6001 ggaaaaaaca gcacaggaaa tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc
     6061 agggttttac ggggcgaacc ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga
     6121 cattaccggc gcgatactgg cgaagatgtt gcaagcggaa agttatgaga attggacgga
     6181 tgtttcagga atcatgatgg ctgatccgcg aatcatcgat catcctaaaa tcatcgagga
     6241 aatctctttt agagaactga gagagatggc ttatatgggg gctaacgttc ttcatgaagc
     6301 agctgttttc ccagttcaag aggctgatat ccccatccaa ataaaaaata ccaatgatcc
     6361 tttcgctcaa gggacaaaaa tatcgaatca cgaaatagaa aaggaaaatg gactgacagg
     6421 aatagctgga cgcaaggatt ttctttccat cactttattc aaacgtcata tgtccgatga
     6481 aatcgggttt atctggaaag caatgagcat ttttgctaaa catgagatat caatcgaaca
     6541 tattccttca gggatcgaca atatcggagt gatcgtctca gcagaagcga ttgccgataa
     6601 gttatttctc atcacgaaag aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat
     6661 cgaagactta gctttgatct ctgtcgttgg gggtccgcat aaagaattga ttggattatc
     6721 tgggaaagtg ttatcgatat tgaacaaatt agaaattcgc acatcgatct tatcacaagg
     6781 agcacaggag ttgaatctga tcatcggtgt accgaataat caatatgaaa cagtagtgaa
     6841 gggtatctat gaagggatgg tgaatacgga tgctgaaaga atatcgaatg gcactccatc
     6901 aaattcctga aatcgggttt catgaattca agacaaaaaa atatttatat gaacaagtaa
     6961 aaaacagcgg ggccgttatc catgaaatcg gcgaaacagg actactcctt tattttgata
     7021 atcagcaaga agaaacgatt gcttttcgaa cagatatcga tgcactgcca gtcaaagaag
     7081 aaacgaatct ctcttttgct tctaagcatg aaggttatat gcatgcatgt ggtcatgacg
     7141 gacatatggc gatgctttta gggttgactg actttataac taaacacatt gaggatctta
     7201 tttataatgt agtactgatc tatcagccat cagaagaaat ttccggtggc gcacaaagcg
     7261 ttattcagtc aggattgttg gataaatatg aagtacaagc aatatttggc tttcatctat
     7321 ggcctggact agctaaggga gaagttttct ctcgaccagg acctttgatg gcacaaagca
     7381 gtgaaacaga cattatcatt aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa
     7441 tcgatagtct ggaggcagct gtacgatttt tgaagaatgt ttatgatttt gaccggcaat
     7501 taccggcaga gtggaatcat ctaatgaaat ttggccaaat ccaaggtgga acgatccgca
     7561 atgttttagc tggagaagtt attgtatccg gaagtatccg ttcttacagt tcagatatgc
     7621 aaaattattt gaagagggga ttagaccatt tagcagaaca gtttcactct acagctacta
     7681 tttcttttcg ctataatgac ggctatccag ctgtaatcaa ccatagaggc ttatatgcag
     7741 cactgttaga ttcaaacaaa ctacatgaat tagatgctcc agttcttcaa gccgaagatt
     7801 ttggcatata tacgcaacaa tatccatgtg tatttttctt tttaggtgtc ggtgataccc
     7861 ctgctttaca tgatccgacg ttcgattttg atatggatgt tttagaaaat ggtgtagaat
     7921 ggtacaaaac gattcttttt acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag
     7981 aggagcgagt aaataatgat gaatttatcc aatcgattta gtcctaggct ttctagaata
     8041 gaagtatcaa aaatacgaca attcgatcag caaatatcgt cgataccaga tgtgatcaaa
     8101 ctcaccctgg gagaaccgga ttttccaact cctgaacatg tcaagcaagc aggaatagca
     8161 gcgattgaag aagacttttc tcactatacc ggaatgagag gattagagga actgcgggaa
     8221 gcggcttgta tatttcagca acagcgttat ggattaactt atgatccgca aacggaagtt
     8281 ttgacgacag ttggtgcaac ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa
     8341 ggagacaagg ttcttattcc agcgcctgct tattcaggat atcagccatt agtggaacta
     8401 gctggtgctg aattgatccc gatagatact tctgacacag ggtttgtctg tcagccggaa
     8461 caatttgaac gagcatttga acaatatgga tcagctgtca aagcagtcat tttaaactat
     8521 cctaacaatc ctactgggac gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt
     8581 ttaaaaaaat atccggtatt tgttataagt gatgaagtat atgctgaatt gacttatagt
     8641 ggaacgcata tgtccatagc tacttatctt ccagaacaaa caatcgtgat aagcgggtta
     8701 tccaagtcac atgcgatgac tggttggcga gtgggcttta tttttgctca aaaagccctt
     8761 attgacgaac taattaaagt ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa
     8821 aaagctgcgg ctgaagcgct aataaatggg gtgaaagata gcgagaatat gaaagagcaa
     8881 tatcaaacac gaagagacta tctggtccag caattggcgc ctttaggatt tgaagtgacc
     8941 cagccaaacg gtgcatttta cttgttttgc aaactcccag ctgccattca aactgactct
     9001 tggcagtttt gtgtagaatt agcggaacag gcaaaagttg cgtgtatccc aggaattgca
     9061 tttggcccag aaggagaagg ttatatccgg atttcttatg ctagcagcat ggaaaaatta
     9121 catgaggcct gcaaacggat caaagacttt ttaacagaac agtgattcat agattttatc
     9181 aaaaaaacaa tcgttaattt tgcggtatat tctgcaagga gacgattgtt ttttaaattt
     9241 agttcaaatg aacgcgtttc ttttgattca taactaatgt aatgttaagt ttaaattgtt
     9301 tagaatgatt ttaaaagaat attcaaggag gtttcatcaa tggaaaaaat caaagttgtc
     9361 attgtaggtg catctcatgg agggcatcaa tcgatcttgg aactattgac acgatacggt
     9421 gaaaatgtag atatcacgct atttgaagca ggagactata tttccttcat gtcatgtggt
     9481 atggagctgt atttagaaga caaggtaaca gatgtaaatg atgtacgtaa tttcagacca
     9541 gaaaactttc cgcaatccaa tgttcatatt ttaaataatc acgaagtaaa aacgatcgat
     9601 gccgataaaa aaacagtaac ggttactagc gcatcagata acgaaacaaa agaatatcct
     9661 tatgacaaat tgattttgag ttctggtgtc aaaccaaaca atctgccagt accaggagca
     9721 gatctagaaa atgtctactt gatgcgtgga tacaattggg caaccaaaat caaagaaaaa
     9781 ttaaatgatc cggctattaa aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa
     9841 gcagcagaag caagccgaaa agctgggaaa gaagtcgtat tgcttgatgt gatcgataga
     9901 cctttaggca cttatttaga tgctgagatg acagatattt tagaacaaca cctaaatgaa
     9961 aacggtattg aagttttgac aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt
    10021 gaggcaatca aaacaagtga aaaagaaatc ccagcagatc tagtcattca agctgccggt
    10081 gtgaaggcaa atacagaatg gttaaaaggg atcgtcgact tagatgaacg tggctggatc
    10141 caaacaaatg aatacttgca aacgaatctt ccagatgtgt atgcagtagg agatgctaca
    10201 cttgcttatt ctatcccagc acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt
    10261 agagaagcac gttatgtagt aaaacatctt tttgaagaag taccaaacaa accgttcggc
    10321 ggagtcgtag gttcctctgc attaagtgtc tttgactatc attttgcagc aagcggattg
    10381 aatagcttta cagcagcaaa agcaggagta gatgtacgag tgtcttatta tgaagatacg
    10441 ttacgaccaa aatatgtacc agaagaacaa ggaaatccaa aagtgtccat ccaattattc
    10501 tcggatccaa tgacccatca attgctagga ggagcagtat tatctaccta tgatgttaca
    10561 ggtcaaggaa atgtattggc actagcgatc caaaacaaac tgactttaga agaactagca
    10621 gaagctgact tcttcttcca gccaggcttt gaccgacaat ggagcatgtt gaatctagcc
    10681 gctcaacaag cgttaggaga agaaccattt gtagaataag aaaacgaaat atttgaaaag
    10741 aggttgtgaa aaagctgcct tgcatcaagt aataagaaca gccaagtaaa aacagcttct
    10801 tatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10861 ttcaaaaaag ggactgtgac acgatttgtg taccgacccc caaaagttag accaaaaaat
    10921 ctaacttttg gaggtcggta ttttttatgg ttaaatatac ttttgaactt aaattaaaga
    10981 tcgttcatga ttatttggat ggcaaaggcg attggaccgt aaaaatccag atcaagagat
    11041 tgaagcggaa atgcttaaaa ttcgggagaa gcacaaggat tatggctgtt tacggatgac
    11101 caatgaacta agaaaccgcg gtttctctat caataaaaag aaagttcagc gcttgatcaa
    11161 gaagctagga ataaaagtaa cggcatacac gcgtaaatca cgtcgctaca attcttatcg
    11221 aggaaaagt
//
LOCUS       DARBFO010000082        10922 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000082 DARBFO010000000
VERSION     DARBFO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.083"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..281)
                     /locus_tag="SCK51_002289"
     CDS             complement(<1..281)
                     /locus_tag="SCK51_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1252876.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
     gene            423..1541
                     /locus_tag="SCK51_002290"
     CDS             423..1541
                     /locus_tag="SCK51_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1252877.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1669..3045
                     /locus_tag="SCK51_002291"
     CDS             1669..3045
                     /locus_tag="SCK51_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEG1252878.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3446..5026
                     /locus_tag="SCK51_002292"
     CDS             3446..5026
                     /locus_tag="SCK51_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEG1252879.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5224..5447
                     /locus_tag="SCK51_002293"
                     /pseudo
     CDS             5224..5447
                     /locus_tag="SCK51_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5652..5972)
                     /locus_tag="SCK51_002294"
     CDS             complement(5652..5972)
                     /locus_tag="SCK51_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEG1252880.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6120..8354)
                     /locus_tag="SCK51_002295"
     CDS             complement(6120..8354)
                     /locus_tag="SCK51_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1252881.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8833..9099
                     /locus_tag="SCK51_002296"
     CDS             8833..9099
                     /locus_tag="SCK51_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEG1252882.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9099..10826
                     /gene="ptsP"
                     /locus_tag="SCK51_002297"
     CDS             9099..10826
                     /gene="ptsP"
                     /locus_tag="SCK51_002297"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEG1252883.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1911 c         2215 g         3317 t
ORIGIN      
        1 gcttcttcaa tcaatatttt tcctaaccct tggtgtctgt attcagaaag aatcgccatc
       61 cgctgtaatt tcatttttcc gttttctaaa ggcaaaagac gtacagttcc ttgcggttct
      121 tttttatctg agtacaggac gaagtggata caatgggctt catctttgtc tatttctcga
      181 gacaaaggca caccctgttc gactacaaaa acttgatgcc tgatccgtac agcatctaag
      241 taaatattgc tcattgtatc ttttgtttgt acaactttca tggtctagcc accttccttt
      301 cgtatagtat actggaaaat aaaacaaata aaaagtatgg caagttttca aaaataaaaa
      361 aagaataaaa gaatttttaa cgaatataat atatactagt aagaaagaaa ggaggaaatc
      421 tattgtttga aaaacttaga cagtctaaac taatgttttg gtcgcttgaa ttattgatca
      481 tagctgcatt gatcatgatt tcatcagaaa taaactttat tttcgaaccg atcgggacat
      541 ttttttccac gttatttgca cctgtactga ttgcaggctt tctttactat ttattgaatc
      601 ctattgtcaa gctgttgatg aaagcaaaaa tcaaaagaat atgggctgtc gcaattgtgc
      661 tgctcctttt agttgcagca attatttgga ttttacttag tgtcatccca agtctagtac
      721 agcaaatttc ctcattggca tctaatatgc ctgatttcat caaacaggta gaaagctggt
      781 taaaagaggt agccgaattg cctttgttca aggaagtaga tatcaataaa tattttgagc
      841 aattagacat ttcttacggg acaatcattc agcaattcct tagtggagta tccaatagtt
      901 taggttcgat cgttggaacg atcgcttcgg caaccgtagt aatcattaca gcaccattca
      961 tcttgttcta tatgttgaaa gacggagaaa aactagtgcc aaacatccaa cagtttttcc
     1021 cagaaaaaag aagaaaccaa attgtggagt tattggggca actgaataaa acattggcta
     1081 attacattag cggacaagca atcgaatgtt tattcgttgg taccttcaca tttttaggat
     1141 atatggtaat tggtgtggat tatgctttct tatttggggt cattgcggga ttgacaaacc
     1201 taattccata tttaggacct tatctaggac tagcacctgc atttctagta acagtcttca
     1261 atgatccggt aaaagcatta ctttgttgtg tggttgtgct aatcgtccaa cagttggatg
     1321 ggaatatcat ttatcctaat gtaattggga aatcactgaa aatccatcca ttaacgatca
     1381 ttatcgttct tctagtagct ggaaatattg ccggattgct tggtatcttc ttaggtgttc
     1441 ctttttacgc aatctgcaag acgatcattt cctatataat caaaatcgtc aaagaagaca
     1501 agcaaaatga aaacaagaag aaaattgcta cgacctcgta ggtagtttta gtacacttgt
     1561 ttttattgca aagaaaattg ctttgtgata ccattttaac gtggctatag tgccacgttt
     1621 ttttctatcg taaactaaaa attaatttta taaggagaga atgcgattat gaatgctgac
     1681 cctgagagtc agtcgctgtt agcgcagatt ttattattga tcgttttgac attgatcaat
     1741 gcttttttag cagcttccga aattgctgtt gtttcgatca ataagaatcg aatcgagcaa
     1801 aaagctgatg aaggagatgt gaaatctcaa aagcttttaa aaatattgca gaatccaaat
     1861 aatttcctat cgacaatcca agtaggaatc actttggtca acattttgtc tggtgcttca
     1921 ctagctaata ctttatcgga acggttagct ccggtattag gtggcggcac agcggcaaaa
     1981 aatattgcaa atatcattgt gctggctatt ttgacctatg tatcgattgt ttttggagaa
     2041 ttgtacccta aacgtattgc tttaaataaa tcagaagaag tcgcaaagtt cacttcaggg
     2101 ctgatacgct tgattggtgt agtagctaag cccttcgtct ggctgctttc tgcttccaca
     2161 agtttattgg cgcgtatcac gccgatgact tttgatgatg aagattcaaa aatgaccaga
     2221 gatgaaatgc gttatatgct tgaaaacgaa ggcgtattga ataatgaaga attagaaatg
     2281 ctccaagggg ttttttctct tgatacgaaa gttgcccgag aagtcatggt cccacgaacg
     2341 gatgcattta tgattgatat caacgacact gtggaagaaa atgtgaatga agttttatcc
     2401 gaaaactatt caagaatccc tgtttataac gaagacaaag ataaagtagt cggaatcctt
     2461 catacaaaga atttactaaa agctgcccat aaattcggct ttgataattt agatatcaag
     2521 aaaatcatgc aggagccttt attcgttcct gaaacggtct ttattgatga tctgttatat
     2581 gaaatgaaaa agacgcagaa tcagatggct attttattag atgaatatgg cggcgtcgtt
     2641 gggttagcaa cactagaaga ccttttagaa gaaatcgtcg gagaaatcga tgatgaatca
     2701 gatgaagttg agaatcttta tgaaaaaatc gatgaacacg aatatatcat ccaaggacgt
     2761 atgctgattg atgagttcaa tgaagctttt gacagtgatc tgcatatgag tgatgtagat
     2821 acgatggctg gttatttgat tacagcatta ggcatgattc cggatgaagg cgaaaagtta
     2881 tcgtttgatg tcgataatat tacgctggtt tctgaagaaa tggaaggttc tcgaatcctt
     2941 aaaatccgtg tcatttttca tgatccggaa gaaacagaag cggaacctga agaagaacga
     3001 agatacttca gaaaagaatt cgaagatgac gagccaagaa gataattgga aacttggtta
     3061 tgaaagacgt gttgcttgaa taaacaagca acacgtcttt tcttttagtc agctgacaaa
     3121 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
     3181 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
     3241 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
     3301 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
     3361 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
     3421 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
     3481 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
     3541 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
     3601 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
     3661 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
     3721 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
     3781 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
     3841 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
     3901 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
     3961 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
     4021 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     4081 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     4141 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     4201 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     4261 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     4321 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     4381 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     4441 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     4501 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     4561 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     4621 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     4681 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     4741 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     4801 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     4861 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     4921 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     4981 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     5041 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     5101 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     5161 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     5221 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaagaa gagatgttac
     5281 gattatgtcg attattagat gtaaaagact gggacaaaaa cgagcaaatt gcacaaaaag
     5341 tacgatcaat catccataaa gaggaaaaag ttaaaaataa agacacgcgt agaactgaaa
     5401 atgaaaagag aaaagtacaa aagaaaagag aaaaatcaaa aagctagaaa gaaaaagagg
     5461 ctgagacaga agcgtttaac tccaagaaat aagctggaat tcacaaaaat ttgaaaagca
     5521 attttcgtga atttcagctt atttccgaag gagttgcttc tgctttcgcc gtttatacgt
     5581 gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctcttta attattatgt
     5641 tcaaaatttg tttacacggc taatgtagcg cctaattcat gttttggaat cgagatttca
     5701 acaaggcctg ccaattcgat tactttgatt tcatcctcgt ggtaaacggc gtctttgatt
     5761 ttatctaaga aataattttt agcatcttcg atttctttcg gctggatttt acgtttgtca
     5821 ttgatcagca gaatttcatt atgttcaggg gcttcagtgt agacaatagt ggtatttccg
     5881 gctgtgtaga ccttgactag atgggcatca gtgttttcaa gctggtttaa tacaagacgg
     5941 gaatggctgt ttgttacatt taccaatttc atggcgctca cctcttttat gtatttttaa
     6001 ttgcttgttt ctcttattat aatttctttt ttataaaatg aacagtattt tgacttttct
     6061 gaaaaaagaa tgagacggca tttctcaaag aaataccgtc tcacgctata aaactggatt
     6121 tattcagccg tttcttcggc tgattcttta gccaaacgtt ctggtttaga agtaacgatg
     6181 atttttccat ctttatccag ttttgctttc aactcatgga tagaagggtg atcaagataa
     6241 aattcagcga taccatcttc gatttgttct tggatcgtgc gtcgaagagg acgagcaccc
     6301 atagatggat catagcctaa gtcaactaat ttttctttga cattcgtagg aacttcgatg
     6361 tgaagttgct gtgctgccag catttggttg acatcgtcta gcattagtgt gacgattgtc
     6421 atcaagtttt cttttgataa tgcactgaat tcgatgatcc cgtcaaaacg gttcagaaat
     6481 tctggtgtaa agtagttgtt taattggttc aatacagaac gagtaacacc ttcgcgtgcc
     6541 gcaccaaaac caacgtttgc ttctacttta ccagttccgg cattacttgt cataatgatg
     6601 atcgtatctt taaagctgac cgttcttcct tgtgcatctg tcaatcgacc atcatctaag
     6661 atttgcaaga acatatgcaa aacatctgga tgagcttttt ctacttcatc taacaagaca
     6721 agactgtaag gatttctccg tactttttct gttaattgac cagcttcttc gtatccgaca
     6781 taacctggag gagaaccaat caatttagag acactatgtt tttccatata ttcagacata
     6841 tcgaaacgaa tcattgaatc ttgtgaaccg aatagttcgt aggcaagttg tttcgcaagc
     6901 tctgttttac ctacaccagt tggtccaaca aataagaagg agccgattgg acgattctgt
     6961 ttgtttaatc caacacggtt tcggcgaatc gctttcgcta ctcgatctac agcgttatct
     7021 tgtccgatga catgagcttt caagtcatct gctaagtttt tcagttgtgt ctgttctttt
     7081 tcttttaatt cacccactgg aatacctgtc cgttgttcaa cgattttttc catatctttt
     7141 tcggtaatca caggtgtttc ctcttcagtg agctgacgtt ctttcatttt ttgaagttta
     7201 ttgatctgat cgcgataata agctgctttt tcgaaatctt cttctcgaga agctaaaact
     7261 ttttgttctt ctgcttcttg cagttttttc tcgattgttt taggatcaac gatttggatc
     7321 gtcaagtttt tcttagaacc tgtttcatct agcaaatcga tggctttatc cggcaagaag
     7381 cgatcttgga cgtaacgatt ggataaagtt gcggctgctt cgatggcctc atcagtatat
     7441 tttacatgat gatagtcttc ataacgtttt tgcaaacctt tcaaaatact gatcgtttct
     7501 tcgacagttg gttcatctac ccgtacaggt tgcatacgac gttctaaagc agcgtctttt
     7561 tcgatgatgc ggtactcatt taaggtagta gcaccaacca tttgcaattc tcctcgagct
     7621 aaagctggtt ttaaaatatt accagcatcc atatttccat caccagcaga accagcaccg
     7681 acgatttcgt gaacttcatc tatgaatagg atcacatttt ctgcttgacg gatctcttca
     7741 atcaattttt gcatacgttc ttcaaattgt cctcgtatac cagtaccttg gactaaagat
     7801 acgacatcta aacgaatgac ttctttgtcc attaattttt gcggtacatc accatctacg
     7861 attttttgtg ctaaaccttc aacaactgct gttttaccaa cacctggttc tccaatcaag
     7921 acaggattgt tcttggttcg gcgatttaaa atttcaatga ctcgtttgat ttcgtcatcg
     7981 cgtccgatca caggatcgat atcaccttgt cgtgcttgtt ctgtgatatt gattccgtat
     8041 tctcccaata aaccttctgt attgccttga ggtggctgac caccattgaa gttgttgttt
     8101 ccgccaaatt gtgttggtgg tgtttgctca taatttcctt gctgttgcat ttggcgagac
     8161 atggaacgga aaagatcgtc caaactgcca aatccaaatg gatcattttg ggccatatta
     8221 gtcagacctc cattttgttg atttttgatt ttttgataac agctctgaca gtaatcgagt
     8281 tgtgtccgct gaccattgac agtggcatac aagtgaatcg ttgcctcatt cttgccgcag
     8341 ttttgacaaa gcatgaatca ttcacgtcct ttcacaaaaa tgggtacatc catattttac
     8401 atctcgtttt tcatgaggta aaagaataac ccctagtttt ttaagagatt tatgaggtag
     8461 aaaaggtgaa atctctattg accattcctg acctttgact cttattatac taaaaataaa
     8521 aagggaagca agtaattcgt ttcatatgaa aacaaagagg gtcaaaggaa agaatgataa
     8581 acgtcaggag aagagaaagc gacaagaaaa aggaaataaa cacgtttgtt ataaaatgtg
     8641 aagaaaagga tgaaaaagaa tccggaataa tttgaatact tttatctgaa attgaatgaa
     8701 aactgttgta tctaacgaaa aaaaatgtta atctttacag atgaaagggt ggcatttgtt
     8761 aggattctct gaattactaa aaaagaaaac cctcacataa aaacaacact cacttaaagg
     8821 agaccgatta atatggaaaa gaaagaattt cacgtagtag cagaaactgg gatccacgct
     8881 cgtccagcta cactattagt acaaacagca agcaaattta actctgatgt aaacttagag
     8941 tataaaggta aatcagtaaa cttgaaatca atcatgggcg ttatgtcttt aggtgtaggt
     9001 caaggttctg atgttactat tactgcagaa ggtgctgacg aagctgatgc aatggcagca
     9061 atcgttgaaa caatgaagaa agaaggctta tctgaataat ggttgaaatg ctaaaaggga
     9121 ttgccgctag tgacggtgta gccgttgcaa aagcttacct gctagttcaa ccggatttaa
     9181 cattcagtaa ggcaacagta gaagatactg cggctgaaga agctcgtttg gatgctgcat
     9241 tagctaaatc tactgaagaa ttgcagcaaa ttcgtgaaaa agcagcgcaa agcttaggtg
     9301 aagcagaagc gcaagtattt gatgcccatt tgatggttct ttcagatcca gaaatggtag
     9361 gtcaaatcaa gcaaaatatt aaagacaaca gcgtaaatgc tgaatcagcg ctaaaagaag
     9421 taacggatat gtatatcggt atgttcgaag caatggaaga taatgcttat atgcaagagc
     9481 gtgctgcaga tattcgtgac gttgctaaac gtattttagc gcacttatta ggcgttactt
     9541 taccaaatcc atcaatgatc aacgaagaag tggtcgttgt tgcacatgac ttgactccaa
     9601 gtgatacagc acaattagat cgcaattttg taaaagcgtt tgtaacagac attggtggac
     9661 gtacttcaca ttctgctatc atggcacgtt ctttagaaat tccagcaatc gttgggacaa
     9721 aagaaattac tgctaaagtc aaagaaggcg atattttggc tgtcaacggg atcgaaggcg
     9781 atgtaatcat cgatcctaca gatgaacaaa aagctgaatt tgaaaaagca ggtgcagatt
     9841 acgcagcaca aaaagcagaa tgggaaaaat taaaaaatgc agaaactgtc acagctgacg
     9901 gcaagcattt tgaattagct gcaaacatcg gaacaccaaa agatctagtt ggtgtccata
     9961 ataatggtgg agaagcagtt ggtctgtacc gtactgaatt cctgtatatg gattctccag
    10021 acttcccaac agaagatgac caatatgaag cttacaaagc tgtattagaa ggtatggaag
    10081 gaaaacctgt agttgttcgt acaatggaca tcggtggaga taaagaacta ccatatctgc
    10141 aattacctca tgaaatgaat ccattcttag gatatcgtgc attgcgtatc agtttatctg
    10201 aacaaggcga cgaaatgttc cgtacacaaa tgcgtgcatt gcttcgtgca tctgttcatg
    10261 gtaatttacg tatcatgttc cctatggtag ctacactcaa agaattccgc gcagcaaaag
    10321 cgatctttga agaagaaaaa caaaaattga taagtgaagg caaggaagta tctgatacga
    10381 tccaagtcgg catcatgatc gagattccag cagcagcagt cttagcagac aagtttgcaa
    10441 aagaagtaga tttcttctct gtaggtacga acgatttgat tcaatacaca atggcagcag
    10501 accgtatgaa cgaacgcgtt tcttacttgt accaaccata taacccatca attttacgtt
    10561 tgatcaaaaa tgtaattgat gcagcacacg ctgaaggaaa atgggcaggt atgtgtggag
    10621 aaatggctgg agatcaaaca gctgttccat tgctagtagg tatgggtcta gatgagttct
    10681 caatgagtgc gacatctatt cttaaaacgc gtagtttaat gaaacgcttg gacacagcga
    10741 aaatggctga acttgctgat cgtgcattga aagaatgcga tatgatggaa gaagtcgttg
    10801 aacttgttca tgaatatgta aaataaacaa cgtatcaaaa gctggtgtgg cttgtaaaag
    10861 agctacgcca gtttttttgt tcttttttaa gaaaacgttt ttgtcagtaa gaatagacaa
    10921 aa
//
LOCUS       DARBFO010000083        10726 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000083 DARBFO010000000
VERSION     DARBFO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.084"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(243..1091)
                     /locus_tag="SCK51_002298"
     CDS             complement(243..1091)
                     /locus_tag="SCK51_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1252884.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1090..2157
                     /gene="gnd"
                     /locus_tag="SCK51_002299"
     CDS             1090..2157
                     /gene="gnd"
                     /locus_tag="SCK51_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEG1252885.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2159..3160
                     /locus_tag="SCK51_002300"
     CDS             2159..3160
                     /locus_tag="SCK51_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEG1252886.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3157..3441
                     /locus_tag="SCK51_002301"
     CDS             3157..3441
                     /locus_tag="SCK51_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEG1252887.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3474..4271
                     /locus_tag="SCK51_002302"
     CDS             3474..4271
                     /locus_tag="SCK51_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1252888.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4285..5118
                     /locus_tag="SCK51_002303"
     CDS             4285..5118
                     /locus_tag="SCK51_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1252889.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5136..5993
                     /locus_tag="SCK51_002304"
     CDS             5136..5993
                     /locus_tag="SCK51_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1252890.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6015..6524
                     /locus_tag="SCK51_002305"
     CDS             6015..6524
                     /locus_tag="SCK51_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1252891.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6561..6962
                     /locus_tag="SCK51_002306"
     CDS             6561..6962
                     /locus_tag="SCK51_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252892.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7074..7427
                     /locus_tag="SCK51_002307"
     CDS             7074..7427
                     /locus_tag="SCK51_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252893.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7461..7904
                     /locus_tag="SCK51_002308"
     CDS             7461..7904
                     /locus_tag="SCK51_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG1252894.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7921..9006
                     /gene="uxuA"
                     /locus_tag="SCK51_002309"
     CDS             7921..9006
                     /gene="uxuA"
                     /locus_tag="SCK51_002309"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG1252895.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8999..9712
                     /locus_tag="SCK51_002310"
     CDS             8999..9712
                     /locus_tag="SCK51_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1252896.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9919..10068)
                     /locus_tag="SCK51_002311"
     CDS             complement(9919..10068)
                     /locus_tag="SCK51_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252897.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10464..10664
                     /locus_tag="SCK51_002312"
     CDS             10464..10664
                     /locus_tag="SCK51_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1252898.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3589 a         1696 c         2320 g         3121 t
ORIGIN      
        1 acaaaaataa taaaaaaaag aaaagataaa ttttttacta ctgtatacta actttgttat
       61 cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat
      121 acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac
      181 taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg
      241 tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct
      301 ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata
      361 cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca
      421 tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca
      481 gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc
      541 aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc
      601 gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca
      661 atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc
      721 gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta
      781 gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg
      841 atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat
      901 ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca
      961 cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg
     1021 gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg
     1081 gagacgctca tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt
     1141 aatgttaaag atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct
     1201 atttacaaaa aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa
     1261 attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac
     1321 catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat
     1381 gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc
     1441 atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca
     1501 aaactcggac caggagacat tattattgaa ggcggaaact cttattataa agattcaatc
     1561 cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga
     1621 ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt
     1681 ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt
     1741 gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg
     1801 caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa
     1861 caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg
     1921 gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca
     1981 ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt
     2041 gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta
     2101 gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat
     2161 gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca
     2221 cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc aagagattcc
     2281 aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc
     2341 attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg
     2401 tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat
     2461 catacctgct ttagttgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc
     2521 catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg
     2581 ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa
     2641 taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta
     2701 tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca
     2761 agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta
     2821 tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag
     2881 tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat
     2941 cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt
     3001 agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga
     3061 agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga
     3121 acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt
     3181 ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt
     3241 tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca
     3301 tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat
     3361 catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa
     3421 aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt
     3481 atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg
     3541 ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag
     3601 aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc
     3661 ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc
     3721 tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg
     3781 cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac
     3841 aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt
     3901 tagttggagg attaggagat aaatacgcaa gaaatgcggc agcacatgct atgcatgacg
     3961 ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg
     4021 ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt
     4081 tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata
     4141 aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc
     4201 ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca
     4261 gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt
     4321 gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta
     4381 cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa
     4441 gcgatgattg ttacagccgc tttgcaaatg atttatatgg ggggtttttc tccaggagga
     4501 tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg
     4561 gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac
     4621 ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta
     4681 gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca
     4741 tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct
     4801 gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga
     4861 gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg
     4921 gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg
     4981 gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg
     5041 aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac
     5101 tatgatgacg gattttaatt taggaggaat gggaaatggc tgaaataatg gataatcaaa
     5161 caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg agatggcatt
     5221 ttgcaaacga aattcctcac tcttttgaac gttacttggc gccatcacta ctatacggaa
     5281 tgatgccaat cttgaaaaaa ctttataaag atgaagactc tttaaaagca gcatataaac
     5341 ggcagttact gttcttcaac actcaattaa gctggggtgg cggtgtgatt actggcctaa
     5401 tgtcttctat ggaacagcaa agagcagaag aagagtatga agaaaaagag atcttgatgc
     5461 aggatgactt gatgtataac acgaaagcgg gattgatggg ggcactagca ggaatcggag
     5521 atgcaatcga ttcaggaacc gtccaatata tttttatcgc aatcgcggta ccttgggcac
     5581 aacagggaag tcctttaggc gcactttttc cttttatctg ctttgggttg tatcaggttt
     5641 tactaggggt atttttcgca agacaagctt tcactatggg ccgtaatgct acaggcctaa
     5701 tgcaaaacac tggtgttcag acagcgattg aaatgctatc agttttaggg ttgtttatga
     5761 tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc attgaaattt gcgatatccg
     5821 gacgtgagtt tgttatccag gaaattcttg atcaaatcgt gccagggata ttacctttag
     5881 ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc acacaagcat
     5941 tattatggct gactggaata ttaattgtgc tagcaggaat cggtatttta taaaaagaag
     6001 ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg
     6061 ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg
     6121 aagttgtagc aaaagataaa tttatgagag atctttacaa aaatgcagga agccgtacag
     6181 gacaaaaaac aattgtcatt acaccagaga aagctaaatt ctattgggat gaatatcaat
     6241 ttaaagaata caattgtatt ttgatcgcaa aaacagtcca aacgatttac gacttagtaa
     6301 aacacggaat ccccatggaa gaattgaata ttggagggat tgctcaaaaa aatcctgata
     6361 cagatctttt agtgacgaaa tctgtttatt taaataaatc agatgctgaa aaattgaaag
     6421 aaatgaatga acaatatggc gtaaagaata tttattttca agccactcca tctgcaccta
     6481 aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa
     6541 aagataaaga gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt
     6601 atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat
     6661 tagtatcaga tatttacagt atgagaacag atgtgacagt cattgcttac gatacgctag
     6721 cctaccgatc aaaaaatatt tatgttggag atatcgaaaa agcagagaaa gttatctgta
     6781 cttattacga tacacccgtt catgcattag gaagttattt catgtttgat tggaaagacc
     6841 aaaggaaaaa aacaatctac tctatattat tgtccttcat tttgttgttc agtttaggtt
     6901 ggtggggaat gatgatatat aacaaaaatc ctcatcacgt attcgatctg ctatccgtat
     6961 aaacaggtat cactgtacta gcatttggga gctacttttt cttattggga aaagcagctc
     7021 gcggatggcc tagcagacaa acttttattc gaaatacttc atccatatta acgatgctag
     7081 aaatgatccg tacgattgat gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt
     7141 atggagaaaa gggcttagac agcgtgagat cgggtatgaa aaaagaggct attctttttt
     7201 atctagactc tgttggggca gatacaccgc tacagttttc gggaaattat ttttcaaata
     7261 aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat tatattttta
     7321 gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa aacagattta cgagggaaaa
     7381 catttaattg gcaaaatgcc aatcagatta ttgctctttt tcgataagta aaaagtgcaa
     7441 aacaaggagt gagaagacta atgttggaaa tcatgatcgt tacacatggt aagttgagcg
     7501 aaggactgaa agatgcggcg gaagtaattg tcgggacatc gagccatatt gaaacaatgt
     7561 gcttagagtc tggggaggac atccaaacat tcggagagaa ggtcaaagaa aaattaacta
     7621 tgatcaacac agcagaaccc atgctagtat ttgttgatct aattagcgca agtccgtaca
     7681 atcaagcgct cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc acttatgtca
     7741 tagggggcgt gaatctgcca atgttgttag aagcaatcaa tcatcagatg cttcacacac
     7801 cgatagaaga agcggtggaa caaattgtca aacaaggaaa agataattta actgtttggc
     7861 atgcttcagc ggaagaaaaa aaattagatg aggacgattt ttagaaggaa ggctgaaaag
     7921 atgaagtggg gatttagatg gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt
     7981 cgccaaatac cgggaataaa tgggataatt ggaactttgc tgactaaaat gcctggagat
     8041 gtttgggaga tttcagaaat tgaggagttg aaagcatctg ttgagagcca aggactggaa
     8101 ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca aggcagggac agatgaacga
     8161 gattactata ttgataacta tatacgaaca atcaaaaact tgtctcgatg tggggttcga
     8221 ttgatttgtt atagcttcaa accaattttt ggatgggcga aaactgattt gtttaatgaa
     8281 aacaacgatg gaagctattc tcttttatac gatcagaaaa tcgtcgatca gatggagcca
     8341 ggagaaatgt atcaattgat acacagccag tcaaagggat tcaaattgcc gggttgggaa
     8401 gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa
     8461 gaattatttg aaaatctgac ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca
     8521 gatgtaaaaa tggctatcca tccagatgat ccgccatggg aactttttgg ttttcctaga
     8581 gtgacgaaaa atcttggcga cttgaagaaa attctaaatg cagtagattc tccatataat
     8641 ggactaacgc tgtgcacagg atctttaggg gcaaatccgg aaaacgattt agttgaaatc
     8701 atccatgagg taggttcgcg catccatttt gtccacttca gaaacgtgga gtttttagga
     8761 gaaagaaagt tcaaagaaac ggcacatccc tctacagaag gctcccttga tatgtatcat
     8821 attatgaaag cgttggtgca agaagggttc gacggtccta tacgccctga tcatggtaga
     8881 accgtgtggg gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc aatgggactc
     8941 acttacatgc aaggtctata tgaggcgata agtaaggaaa aaccactggg agagcattat
     9001 gaataaaatc aagctcttca tctttgatat ggatggcctt ttattagaaa ctggacgttt
     9061 agcttatcgt gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt
     9121 ctattattta ctaactggcc aaacagaaat ggcgataaga gaacaaatgg gcattttgta
     9181 cggagaagat gttccatata tcaaatggcg agaagcaatc aatcaatata aagaaaaaat
     9241 cgtgaaagaa gataagagag tatatacgaa aaaaggagca gaagaaattt tatcttttgc
     9301 aaaagaaaga ggaatccata caatcgtggc ttcgtctaat acacgggtaa aagtagaaat
     9361 gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa atcatttcag gggatgatgt
     9421 aaaaaaagga aagccggaac cagagatctt tttgaaagct tgttcaaaaa tgaatattcc
     9481 tccaagtgaa gcacttgtat ttgaagactc gattgcaggg atcgaagcag caaggcgagc
     9541 gggcattttg tcttttctca tcaaagatca tttgataggg ttgcccgatc ataatgggaa
     9601 gcataagttg aaagtagatg taagtttata tgaatccaat aaagcagact atgaatttta
     9661 tgacctgcat caagcaaaac gatttttaga aagtaaaggg ttgtatctct aataaagagg
     9721 actattttct agtcctcttt atttattgtc ctgcacgact gagggaacag atgaaaaaga
     9781 agtaattttt tattttgaag tatctacctt atttgtagtg aacaatttag aacaaacaca
     9841 aaacgcatct actgttatct accagtagat gcgtttaaag agaagaacga gaatcgtcca
     9901 tatgagggtt tccactcctt atgcagattt ttttaattct tgcactgctt gttcgatcgt
     9961 aacacctgtc gctacatgat tggtattttc tttgtcaaaa acgacaaata gattcaaatc
    10021 tgtatccaag gttactcgat attgtatatc tttgttatag aatgtcatag ccattctcct
    10081 ttctttaaat acttgctgaa aggcaacgaa cgcgttgata acgaaattcc atttataagt
    10141 ataccaccaa ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag ttgcagataa
    10201 aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga
    10261 ttcgaagaca atagaagaca atgaatttta tatttcattg cattatcctt gtgttattag
    10321 aaaaagcatg atatattaaa tgagtaatct attttgaaac gtaatttgag cgatatatct
    10381 acatagataa aactcctttt gcctatcaaa tatttattgc aacgaaaaaa cgtacattga
    10441 tacgtatcag gaggaattta tttatgaata acggtacagt aaaatggttc aacgcagaca
    10501 aaggttttgg ctttatcact ggtgaagatg gaaatgatgt atttgcacat ttttcagcta
    10561 tccaaggtga cggcttcaaa actttagaag aaggccaagc agtgtcttac gatgttgaag
    10621 atggtcaacg tggccctcaa gcaacaaaca ttgttaaagc ttaataaaaa ttgactttta
    10681 accgtctttt cgaaagaaaa gacggttttt ttgtacgaaa aaattc
//
LOCUS       DARBFO010000084        10708 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000084 DARBFO010000000
VERSION     DARBFO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.085"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(25..162)
                     /locus_tag="SCK51_002313"
     CDS             complement(25..162)
                     /locus_tag="SCK51_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEG1252899.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="SCK51_002314"
     CDS             complement(159..1343)
                     /locus_tag="SCK51_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEG1252900.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="SCK51_002315"
     CDS             complement(1599..1853)
                     /locus_tag="SCK51_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252901.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="SCK51_002316"
     CDS             complement(1878..3020)
                     /locus_tag="SCK51_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEG1252902.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="SCK51_002317"
     CDS             complement(3080..4432)
                     /locus_tag="SCK51_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG1252903.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="SCK51_002318"
     CDS             complement(4503..4847)
                     /locus_tag="SCK51_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEG1252904.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="SCK51_002319"
     CDS             complement(4857..5231)
                     /locus_tag="SCK51_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1252905.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="SCK51_002320"
     CDS             complement(5244..5558)
                     /locus_tag="SCK51_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1252906.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="SCK51_002321"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="SCK51_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="SCK51_002322"
     CDS             complement(8976..9638)
                     /locus_tag="SCK51_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252907.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="SCK51_002323"
     CDS             10255..10485
                     /locus_tag="SCK51_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252908.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT         2957 a         2366 c         1814 g         3571 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactga
//
LOCUS       DARBFO010000085        10674 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000085 DARBFO010000000
VERSION     DARBFO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.086"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            202..2433
                     /gene="pflB"
                     /locus_tag="SCK51_002324"
     CDS             202..2433
                     /gene="pflB"
                     /locus_tag="SCK51_002324"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEG1252909.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            2677..3438
                     /gene="pflA"
                     /locus_tag="SCK51_002325"
     CDS             2677..3438
                     /gene="pflA"
                     /locus_tag="SCK51_002325"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEG1252910.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            3523..4449
                     /locus_tag="SCK51_002326"
     CDS             3523..4449
                     /locus_tag="SCK51_002326"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEG1252911.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            4631..5524
                     /locus_tag="SCK51_002327"
     CDS             4631..5524
                     /locus_tag="SCK51_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEG1252912.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            5658..5783
                     /locus_tag="SCK51_002328"
     CDS             5658..5783
                     /locus_tag="SCK51_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1252913.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5917..6549)
                     /locus_tag="SCK51_002329"
     CDS             complement(5917..6549)
                     /locus_tag="SCK51_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252914.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(6697..7239)
                     /locus_tag="SCK51_002330"
     CDS             complement(6697..7239)
                     /locus_tag="SCK51_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEG1252915.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            7374..7763
                     /locus_tag="SCK51_002331"
     CDS             7374..7763
                     /locus_tag="SCK51_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252916.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            7767..8531
                     /locus_tag="SCK51_002332"
     CDS             7767..8531
                     /locus_tag="SCK51_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1252917.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            8660..9607
                     /locus_tag="SCK51_002333"
     CDS             8660..9607
                     /locus_tag="SCK51_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1252918.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            9737..10477
                     /locus_tag="SCK51_002334"
     CDS             9737..10477
                     /locus_tag="SCK51_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252919.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         3760 a         1788 c         2119 g         3007 t
ORIGIN      
        1 aatccttata cgtttgcaaa aaaagatgaa agtgttcaca atgcttaaat gttggtatgt
       61 caacgttgat ttaaatatat gtaaataaaa cataaaaatt gttaaataaa aaacaagaaa
      121 acccttgcaa agatagggtg ctgtgttata ctaatcacgt aaaaactgtt acataaaaca
      181 gaaatttagg aggtccatac aatggagcaa tggaatggat ttaaaggtac caaatggaaa
      241 gaaggcgtag acactagaga ctttattcaa aataactaca ctgaatatcg cggtgatgac
      301 agctttttgg aaggcccaac agaagcaaca gaaaaattat gggatgaatt gcaaaagtta
      361 aatgatatcc aattcgagcg taacggtgtc tatgatatgg acacagaaat cgtatcaact
      421 attacttcac acgatgcagg ctatttggat aaagatttgg aaaaaatcgt cggtctacag
      481 acagacaaac ctttgaaaca agcattcatg ccgttcggcg gaatcaatat ggctaacaat
      541 gcgctaaaaa gcaatggcta tacaccaaac gatgaattga cacatatttt cactgactgg
      601 cgcaaaacac ataatcaagg tgtatttgac gcttatactg ctgaaatgcg tgctgctcgt
      661 aaaaacaaaa tcattacagg gcttccagac gcttacggtc gcggacgtat catcggtgac
      721 tatcgtcgtg tggcattgta tggaatcgac tatttgatgg aacaaaagaa aaaagatcat
      781 gatatgactg gttatcatac aatgagtgaa gaagtgattc gtttacgtga agaaatcact
      841 gaacaatatc gtgcattagc gcaaatgaaa gaaatggctg cgaaatatgg ctatgatatt
      901 tctaaaccag ctgcaaatgc aaaagaagct gtccaatggc tatacttcgg atatttagca
      961 gcaatcaaat cacaaaatgg tgctgcgatg tcaatcggac gtatctcagc attccttgat
     1021 atctatatcc aacgtgattt ggataatgga gtaatcacag aaaaagaagc acaagaattg
     1081 atcgaccact tagtaatgaa attacgtatg gtcaaattcg cgcgtacacc tgaatataac
     1141 caattattct caggattccc tatctgggct actttatcca ttgcaggtat gggattagat
     1201 ggtcgttcat tagttacaaa gaatgatttc cgtctgcttc atacattaac aaatatggga
     1261 ccttcaccag aaccaaactt gactgttttg tattctgaac accttccaga aggattcaga
     1321 acatatgcta gcagaatcgc tatcgaaagt tcttcgattc aattcgaaaa tgataatcag
     1381 cttcgtgaac aatggggttc tgatgattgt gcgattgctt gctgtgtatc aggaacagtt
     1441 gtcggaaaag acatgcaatt ctttggcgct cgtgctaact tggctaaaac actattatat
     1501 gccatcaatg gcggagtcga tgaagtgact ggcgcccaag ttggaccaga atatcgtcca
     1561 atcacatcag aaatactaga ctttgaagaa gtgaaacata acttcatgaa tatgatggat
     1621 tggttagcag aactatatgt caatacgttg aacatcattc attatatgca tgataaatat
     1681 tcttatgaag cagctcagct ggctttgatg gattctgact tgcaacgcac atttgctaca
     1741 ggtattgctg ggatctctca tgcggcagac agcttgtcag cgatcaaata tgcaaaagta
     1801 aaagcaatcc gtaatgaaaa aggaatagca gttgattttg aagtggatgg cgacttccct
     1861 aaatacggaa atgatgatga ccgtgcagat gaattagttt catggatctt ggaatacttc
     1921 atgggacaaa tcaaacgtca taaaacttac cgcaactcaa aaccaacaac ttcattattg
     1981 acgattactt caaatgttgt ttacggtaaa aatacaggaa atactcctga tggccgtgaa
     2041 gctggaaaac cacttgcgcc aggagcaaat ccaagttaca atgcggaaca aagtgggtta
     2101 ttagctagcc tgaactcaac agctaaaatg aattacggct gtgcattgga tggaatttca
     2161 aatacccaaa caatcaatcc ttcaggtctt ggaaaagaac aagatacaag aatcgaaaac
     2221 ttgcgtaatg tcttagatgg atacttctca aaaggagctt atcatttgaa tgtcaatgta
     2281 ttcagtaatg atttattgcg tgatgcaatg gaaaatccaa tgaaatatcc gaacttgacg
     2341 atccgggtat ctggttatgc agttaaattc cgtgacttga caagagaaca acaattagat
     2401 gtcttgatga gaacttcaca cgacagaatg taaaaagttc gtgaaaaaag tttttcgact
     2461 tagcgtcgaa aggacgttga atagccatcg catcaaaatc gaataatcag tatgcttatt
     2521 tgaaagaaac gatttacagg ggcaggactt gtatgttccg tccctgtaaa ttttacacat
     2581 aacgaagcgg caattgcgaa aaattttgtt cagaaagctt cgcttcgaca gctaacttgt
     2641 acttaaaatg aaatcaaaga aaagatgtga acttaaatgg aagaaaaaac aatcggctat
     2701 gttcattcta tcgaaacttt cggttcagta gacggacctg gactgcgctt tgtcgtgttt
     2761 atgcaaggat gccgcatgcg ttgccaattt tgtcacaatc cagatacttg gaatattggc
     2821 ggagggaaag aatacacagc agatgaactt ttagataaag cagaacgctt cagaccttac
     2881 tggggagata aaggtggtat tactgttagt ggtggagaac ctctattgca gatcgatttt
     2941 ttgatcgaac tatttaaaaa agcaaaagag cggaagatgc atactacatt ggatacatgc
     3001 ggaaaacctt ttacgtatga agaacctttc ttttcacgtt ttcaagaatt gatgaagtat
     3061 acagatttac tgttatttga catcaaacac atcgataatg aagagcataa aaaactaacg
     3121 cattgggaca atgaaaatat tttagaaatg gctcaatatt tgtcaaaaat caacaaaccg
     3181 gtttggattc gccatgtact agttcctgag cgaagtgatt atgatgaata tttgatccga
     3241 ctagacaact ttattcaaac cttgtcaaat gtagacagag tagagattct tccttatcat
     3301 acgatgggaa aatacaagtg ggaaacactt ggtttgaaat atccattaga agggatcgaa
     3361 ccacctacaa aagaacgtgt cgaaaatgct aaacgattgc ttcatacaga agagtataca
     3421 aaatatttaa cgcgataggt cgcatcggaa agaagataag tattgcttct ttccgttttt
     3481 ttgctattat taaataggag aaatcacaaa ggagcgaatg acatgtcaaa aattttagtt
     3541 tttggtcacc aaaatccaga tacagatgca atcggggcag ccattgcgtt ttcacacttg
     3601 caaaatgaat taggtaaaga aacagaagca gttgctctag gtacacctaa tgaagaaaca
     3661 caatacgcat tagaccactt tcagctatct gcaccacgag tagtagagtc agctaaagca
     3721 gaaaatgcag atgtcatgct tgtggatcat aatgaattcc aacaaagtat ttcagatatc
     3781 agcgaagtaa atattttagc tgtagtagac caccatcgta ttgcaaactt tgaaacagct
     3841 aatccattgt actatcgtgc agaaccagtt ggatgtacaa gtacgattat tttaaaacta
     3901 tataaagaaa accaagtaac aattcctaaa gaaatcgcag gaatcatgct ctcagctatc
     3961 atttcagata cattgttgtt caaatcacca acatgtacgc ctgaagatga agcagctgca
     4021 aaagaacttg cagcaatcgc tgaagtcgat ctaaacactt atggacttga aatgttgaaa
     4081 gcaggaacaa atctaagtga taaatcagct gcggttcttc ttgatttgga tgcaaaaagt
     4141 ttcccaatgg gcagttccaa cgttcgtatc gctcaagtca atacagtgga ccttaatgaa
     4201 gtgatggatc gacaagcaga gctagaagca gcaatggaag atgaaatgaa agcaaatggc
     4261 tacgatctgt tcgtattagt tgtcacaaac atcttagaca gtgattcaga attacttgtt
     4321 gctggtaaag gaaaagaaaa agtagaagaa gctttccaaa caactttgac caacaaccgt
     4381 gctttgctta aaggtgtcgt ttcacgtaaa aaacaagtcg tacctcaatt gacggctgta
     4441 tttagctaat caaatggtag tatgaagcat tcaaaatgaa tggataagcg attgatataa
     4501 aaagaaacat agaacatact aaaggccgaa gaaatggagg agaataacag tcaattatga
     4561 ctcttgttct tcctcctttt tacgtaaaag ccttttaagt aaaataaaac ggatagaaaa
     4621 gagaacatat atgaactatt attcaaaaaa taaggaaaac ttttatcaaa aactgaccga
     4681 tgatccgctt ttttcttcat tgaccgatta tctatatgaa catcgagaac aagaaacgat
     4741 tctaagagaa ttgaaaaaag aattttcgca aaataaattt tcccattttc ttgatttatt
     4801 gatcgatgca ggattaatca agagggaaga acgaagatat catttgaact ttccaatatt
     4861 tgatcctaaa gattacttgc aacaagccac ttcggctgct gaaaccatag ctgaacaatt
     4921 gaagagactc tctgttgatg aacaaaaatt agcgatggga gaagttattt gggcgtattg
     4981 tttcgaagat gaacggaaag aagcgtattt ttatggagta agaaacagcc gagagacaga
     5041 attgctgagg acaactgcag gaaatgagaa gtaccgtttt atcacacttt cctctataga
     5101 acacttcccg cttactttgg ctaattactt ttttgtacag aagaatcagc ttcctgtaac
     5161 gaaggcattc aaagaattag ccgaacttat tggagacgta aatgaatcgt acttttttga
     5221 tcaaatagaa gttatcgttg acaggatacg taagaataaa tataaaaatc gccggcctag
     5281 tattttccat cagtctctac ttgtgacgaa tactataaag gaagaagaat cattcacatt
     5341 agaattacct attgtcgaaa aaaacaattt tgagattgaa ttaccaacat tggatccttc
     5401 attgacaatg gaggaaatgg cctttcttaa acgacaaatc ttttctgagc tgagcaagaa
     5461 atttataccg catgcatttt cttatatcaa agaatatgga acagttttag tgagtaaaac
     5521 ctaagagagc agtaagcaaa aattatactg actcttgcag gaggagcctt tctcttcctt
     5581 tatggttaaa agaattattt gagtattttg cctatctacc acctattaga ttcactacat
     5641 ggtctggaaa caaaggaatg caaaaaaatg gtgagaaatg tggaatgaca aaggaagtag
     5701 tgataagaaa agttagattt ttaaataatc aatattatga ttcagtcaag tatggcattt
     5761 tatgggaaga actagctgat tagattagga agtccatttg ataatccaga atcactaaga
     5821 aacgaatggc gagtcattaa taatttatcc aatcttgggt tattctttta gattgaagtg
     5881 tttaaaacta aaaaagcatt tatgaagtcg taatagtcag gcttcaaaat gctttttatt
     5941 ttgtttgttt tttctttgcc acataaatac acctagactg acgatgaaga gcatatccaa
     6001 cagatttcct gcgacttgga tcgtcgaagg gttggtcagt ggcattaaag caaggacaaa
     6061 aaaggaaaac aagctgtaaa taaaacaggt gacaggaact aatcgaaaga agagcggaca
     6121 tttcaattgg ctaaaaatga gtgaaaaaat aaaataagta aaaatggata aaatcacgac
     6181 taaaccattc atgataaaga agaaaagcgt attgtgatta aacgaataaa aaagaattgc
     6241 taaaaaagaa aacgttttaa atgcaatcaa tacgcttgaa gcttcgttta acgtaccagg
     6301 atgtcgatga aaaaaatagg caccgaaata aacagaaaac agtaagccgc aaagagaaga
     6361 caaaatcaaa aaaaacggat tttcttcggc ttctgtgata tcagaatgat ccaaaagcaa
     6421 ccagcctaat gcactagaag gcgcatgtga ggtcttaagg tatgaataac atagaaaaaa
     6481 taagatcgtc aaagttgcaa aaaaagagcc taaatgtaaa aaattagtgg ttctacgttg
     6541 aatttccata atatctccaa attaaattga taagtatata aagtataata acaaattgat
     6601 gagtatatgt cgatgttttt taatcaaaat aataaataaa aactgtctaa catttaaaat
     6661 ttaatttaga tattagacag tttttaagtt tgtgagttat tttccttgtc gttttctcac
     6721 ttttttagtc cagaaaccac ctgaaatata ttttggctca taagaaatga taaaagcacg
     6781 ctcttcgatt tctttgatca ggcgatacag ttcacgttcg ctttttctag gagaaagtat
     6841 ttcgagaact agccgctcac cttccagccc cattgcagaa gtctgtgtca caccataacc
     6901 gtgttctctc agtatacccg gcaaactttt atcttctgcg ctatttgctg gcaaaatcgt
     6961 tgtgaccatg atgtatccta atgccaattt gtcttcaatc aaaattccga cactgatacc
     7021 tactgcgtaa cctaacgcat agacaaataa attcagtgga ttatctaaac gatctaaaac
     7081 aagggataag ccgacgatat aaatcgtgat ctcgaccata ctgacaagtg gagcaatcag
     7141 tcgatagcct ttcattgtca acatgaaacg taacgtgttc agagtgatat aagcaaaatt
     7201 aatgaggaag atcatcccca acatttttgg atcaatcatt tgtaaaactc ctttcattta
     7261 tataacaaca atatcaaaaa aaatcgtaag tgaagaaaaa ttgaaacttt atacatacaa
     7321 aaaaatgata aaatgattat aatagaatta taaagatagg ttaggtgatt aaaatgaaaa
     7381 cggtcaatcg tgtgttttgt gccagccacg tgctaagtct ccttctcagt atatacttag
     7441 gtgtctattc gattcaacag ccaattgagg tagagaacat tgtttttcag atgtcgctga
     7501 cagatggttt aattttaagc gtttttttct ttttgatact gtttattggt aatattttcg
     7561 gtagcgttgc cagttttttg aatttttcta tttatccact cctttcgcta gcattaggag
     7621 tggtgggact ttgttctctg tgcttatttc ccgagccatt cagtcctgca tttatattat
     7681 ttggtacact gaacctgttc cagacaactg ttggagcatg gttgctatgg cgatcaggaa
     7741 atttaatgaa aataggtgag taacaattga atccaattat attgaaacgt tctgtccatc
     7801 atattccagt gttagaagtt gttctgaaag aattaagaga ggaacgtatt cccgttgtca
     7861 tctattatca cggatggcag acaagtaagg agttagtttt gacgcaagga agaaaaattg
     7921 cgcagcaggg cattcgtgtg atcctgccag atgcgatgaa tcatggggag cgcaaacagc
     7981 cggtttcttc tattccatcc tatacatttt gggatagtat ctacggaaat ctgtttgaat
     8041 ttgacacact tatcaagcat ttagaaaaaa gaggtcttct acatgatcag cttgcagttg
     8101 gcggggtttc gatgggggga atgacaacat gcgccttgtt ggctaaacat cctcatatag
     8161 tagcgggcgt gtgtttgatg ggttcccctg cacctttgga atatggaaaa gaaatcgtaa
     8221 ggcgcgcatc ggaatataat tatgtcgtac caaaagacta ttttgatttg atttcatggg
     8281 ttaagagcta tgatttgtca ttgcagccgg aaaagttagc tggacgtcct ctgttctttt
     8341 ggcatggaca agctgacgaa aaaatccctt atatacaaac ggaacgattt gtagaaaaaa
     8401 atcggcataa gtcttacgga aaagaaattg tttttcgttc ctcaaaaaca cagcgacata
     8461 tggtacaaat accactaatg gaagaagctt ccactttttt agcaaaaaat ttgataaaaa
     8521 agtataaata attgaaacgg aaaatcactc atgacgatag aggattttcc gttttttatt
     8581 ttgggctata ataaactctg ttaggaaaat ttggtataat tgatagtaag aatgacaaca
     8641 tgttagaaag gaagagagca tgcccacttt agcagatgtt gcaaaacgtg ccaatgtttc
     8701 aaaaatgacg gtctctcgag tcatcaatca tccggaacag gtgactgacg aattgaaaga
     8761 gttagttttc tcagcaatgg ctgaactgga ttaccgtcca aatattgctg caaaagcact
     8821 tgtcacgaat cggtcgcaga tcatcaaatt atttatactg gaagaaatcg acacaactga
     8881 gccctattac atgaatttgc tgatgggaat cgctaaacgg atcggtaaag cgcattattc
     8941 tctccaatta gttacaaatg atgccttcga cgtcggttct tgtgatggat acatcatcac
     9001 aggtgtcaga aaaaaagact tcgattggat cgaccgtttg gaaaaaccag ttgtcttgtt
     9061 tggggaaaat tctgaaggat atgattatgt agacagcgat aatgaatatg gtacagcaaa
     9121 agcaacgcaa tatgccttag aaagagatta tcaaacgatt atttatatcg gtatcgaagt
     9181 ggacgagcct tttgaaattt caagggaaac tggttattta tctgtgatga aggaagttga
     9241 tcttcctagt aaaatcttgc ggtttccaaa tcattcgaca gaagtagaaa gatatatgac
     9301 agaaaactgg gcagatttta cagataaaac ttgttttgta tgtagttcag atcggctagc
     9361 actaggagca gtaagaggga ttataaatgc tggaggacac ttgcccgaag aatttggtgt
     9421 gattggattt gatggggtat ttttagacca aatcagttca cctaaattga caacgatcaa
     9481 gcaagatatt atacgtatgg gtgaagtatg tggagaaatg ttgttaaaaa aaatagaaga
     9541 aaaaggggcg agtcaaggtt atcgtcgttt ttttcctgaa ctagttatca gagagacaac
     9601 gaattaagtc ttattactat atatatattt atgaaattca tcgctatttt cttaaaataa
     9661 tcagcgcatt gctactgaaa acaatgaatc tgtgctatta ttgagaaaag ttccattttt
     9721 gagaaggatg gtttgaatga aagaaatcag acgaaaacat ttccgttttc ctgcgtacga
     9781 tgatgagtta ggcgtaaaat taaccaacga caagaagcga gtattatttg acggacaaga
     9841 agatcttttg actgaacaga atcagtcccc gtttgaacag atgaaagaat ttcatttcac
     9901 gaacacgaac accgagaaaa tccgtcgcaa gcgtcgtgca acaaatggat tgaagtcagc
     9961 tccgcgtaag gaaaatttgg aaaagcataa aggaaaatta ccggattata ccggccatta
    10021 ccaatcagaa atcacatcga caggacgaaa aaaacttttt ggtgattcct ctcgttctgt
    10081 cagacaaagt gaaacaagga cagagtccaa gcatactaca cgaaacgcat ctgttgacac
    10141 gaaggtgcgg gagcgttctt attttgttcc taaatatgta cccgcatcga ttatcccaga
    10201 ccctagagaa agcaaaattt ctgaggatca tttaatcgaa tcgatgaaaa aaacggatga
    10261 ctcttatctg ttgttcgata cagaaccgac gccgtatcaa gaaaaaagaa atggtgagcc
    10321 gtctgtacga acattcaatc gaacaaaaac gttagaggaa acaaagcagt cagaagaaaa
    10381 aaaatcgaaa aaaagccatg gtatcttaga acgctcatta caaggattga tcgatgatca
    10441 aggaaactct ttgggagaaa atagctattt taaataagtg atatacaaat ttatattcgg
    10501 ctacagttgg aaaggaaacg ctgcggcgtt tccttatttt atactctatc cgaaagttta
    10561 tatcgtatcc tcaaattttt ttgagtttaa gcgaattttt tctcccaata agtaagaaaa
    10621 taatggcaaa ataaaaaaac aaactatttt cgcagatcgt ttgcaaaaat agtt
//
LOCUS       DARBFO010000086        10280 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000086 DARBFO010000000
VERSION     DARBFO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.087"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            170..319
                     /locus_tag="SCK51_002335"
     CDS             170..319
                     /locus_tag="SCK51_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEG1252920.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            330..1844
                     /gene="dltA"
                     /locus_tag="SCK51_002336"
     CDS             330..1844
                     /gene="dltA"
                     /locus_tag="SCK51_002336"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEG1252921.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1841..3046
                     /gene="dltB"
                     /locus_tag="SCK51_002337"
     CDS             1841..3046
                     /gene="dltB"
                     /locus_tag="SCK51_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEG1252922.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3091..3324
                     /gene="dltC"
                     /locus_tag="SCK51_002338"
     CDS             3091..3324
                     /gene="dltC"
                     /locus_tag="SCK51_002338"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEG1252923.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3327..4595
                     /gene="dltD"
                     /locus_tag="SCK51_002339"
     CDS             3327..4595
                     /gene="dltD"
                     /locus_tag="SCK51_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEG1252924.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4736..5818
                     /locus_tag="SCK51_002340"
     CDS             4736..5818
                     /locus_tag="SCK51_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEG1252925.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5818..6621
                     /locus_tag="SCK51_002341"
     CDS             5818..6621
                     /locus_tag="SCK51_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEG1252926.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6691..7431
                     /locus_tag="SCK51_002342"
     CDS             6691..7431
                     /locus_tag="SCK51_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG1252927.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7442..8446
                     /locus_tag="SCK51_002343"
     CDS             7442..8446
                     /locus_tag="SCK51_002343"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEG1252928.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8491..10188)
                     /locus_tag="SCK51_002344"
     CDS             complement(8491..10188)
                     /locus_tag="SCK51_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252929.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3305 a         1701 c         2084 g         3190 t
ORIGIN      
        1 taaaagtacg agaaattata aagaaaaaag cagtgatttg atgtgctatt tttataaaag
       61 ctatgtgaca aaactgttag taaaaaaacg gtttctgtaa gctagacaca tgtttctttt
      121 tgtcaaaatt agttacactt tgtgtgaggt tatatgagga gggaatccaa tgaacaaatg
      181 gcgttctaat ccaaaattga attattgggg aaaattcatc attcgaacaa ttttttatag
      241 tgcagtactg ctgttcctga tttatctgta tcactataaa aatattcaag gaggaacttt
      301 tatctataac gaattttagg aggaagagta tggaaatcaa aacgattatt gaagcaatcg
      361 atgattgggg aataaatgaa cctgatcgag ttgcttatca ggcggatgag acgcatactt
      421 ttggtgaatt gaaacaagca tcagacgcac ttgcttatta tttagaaaaa aaggtagaag
      481 gcgatggacc aattgtcgtt tttggcaatt tggagtttga aatgattgtc tcatttctag
      541 gggttgtcaa atctggccat gcctatttgc cgattgaaga gcatacaccc aaggagcgta
      601 tcctatcgat ctttcgggtg gctaaaccat ctatggtcat cagtatcggg gactggatag
      661 aagatattcc ttcggttcct gtcataacaa aaaaagaatt tcaagaaatc aagaagatac
      721 cagtaggatt ttatcccaga aatagcgtaa aaggtacaga aaatttttat attattttta
      781 cttctggtac tactggagaa ccaaaaggcg tacaaatcag tcatgataat ctagtcagct
      841 ttgttcaatg gacgatggaa gactttggga tcaagcctgg tatgcatttc ttagcccaag
      901 cgccattttc ttttgattta tcggtatttt ctatttatcc tgcattagtt tctggcggaa
      961 tgctgaagcc gttaaataaa gcagttgttc aagactttcg tcaattattc gcgacattgc
     1021 cagccttgaa gttgaatgtg tgggtttcta ctccatcatt tatggatatt tgcttgatgg
     1081 aacctacatt caatggagaa aatgtgccgg aattggacat gtttttattt tgcggagaag
     1141 aattgacgaa aaaaactgca gaaagtctac ttgatcgatt cccaaatgca cggatatata
     1201 atacttacgg tccaacagaa gcaactgtag ctatttcaag tattcagatt gaccagcatg
     1261 tactagatag ttatgaccga ctaccgattg gctacgtaaa aaaagacacg aaagtttcta
     1321 ttgtccaaga aggtaaacct gtttcaaaag gagaaacggg tgaaatcatt attgcaggac
     1381 caagcgtatc aaaaggttac ctgaacaatc ccgaaaagac agctgctgcg ttcttttgtc
     1441 aaaatgactt agcaagttac catacagggg atgcaggtag actagatgaa tatgggcttt
     1501 tattttatga aggacgtatg gatcagcagg tcaaattaca tggataccgg attgaactcg
     1561 gggatatcga acattatctg ctccaagaca accgaatcaa acaagctgtc gtcgtaccaa
     1621 aataccaagg aacaaaagta cagcagctgg ttgcttttgt cgtattagaa aacaaaacga
     1681 aacagccgga ttttcagttg actaaatcta tcaaagaaca attactagaa gttgtaatgg
     1741 attatatgat tcctcagaag ttccattatg tcgatctgct gcctcagaca gttaatggaa
     1801 aaatcgatcg aaaaaaatta acagctgagg tgaatcctga atgatgttac ctcatatgat
     1861 cccttatgca gagccgtttt atttttttct tttagccatt gcgcttgtac cgatcgttct
     1921 atctttattg atatgggaaa aaagattacc agtttatcaa tcagcagtga cacttttctt
     1981 tttatttgtc agctttggag gtaattattg gcaacaggga gcggcgctga ttgggtatgt
     2041 tttgtggcag acgatacttg tatggggcta ttttgtttat cggcaaagga aaaatcagac
     2101 aggatggttt tttgccgccg ttattttagc gataattcca ctgtttttaa cgaaagtgac
     2161 tccttttttt gaacatggaa aagcatcgtt acttggtttt ttaggaatct cttatttaac
     2221 ctttaaatct gtacaaatga tcatggaaat aagagatggc atgatcaaaa catataagcc
     2281 atttcgttat atccagtttc tgctgttctt tccaacaatc tcttctggac caatcgatcg
     2341 ctataggaga tttgaaaaag atcagctcca tccgcctgtt cgtgataaat atgttgatct
     2401 tttgggaaaa ggcgtccaaa atatttttgt cggtttttta tataaattca ttatcggtta
     2461 ttattttggt caagtactgc ttcctgtagt gggacgcatt gccttacagc atggtggtat
     2521 ttcatgggca ttagtaggtt atatgtacgt gtacagtatg tatttgtttt ttgattttgc
     2581 tggctatagc ttatttgcag tcggtacaag ctacatgatg ggatatcaga caccaatcaa
     2641 tttcaataaa ccttttttat catggaatat caaagaattc tggaatcgtt ggcatatgac
     2701 gctgtccttt tggttccgtg attatgtata tatgcgattg atgttcttca tgatgaagaa
     2761 aaaatttttt aaaagcaaga tcgtaatgtc aaatatcggt tattttgctt tgtttcttct
     2821 aatgggtata tggcatggat tgacgtggta ctacatcgta tatggactgt atcatgcttg
     2881 tctgatctgt ctgacagatg catggatccg atttaagaag aagcataaag agcagttgcc
     2941 ttctaacaaa tttacgcatg gcttagccgt atttttgaca tttcaagcag tatgttttag
     3001 cttcctgatt ttttcaggat ttttagataa actttttttt agctaacgaa tagtgacata
     3061 ttaatttata taacaaagga gaaaaggaaa atggaagaac aaattttatc aatcttagaa
     3121 gaaataacag gaacagatga agtaagaaaa aatcgtgata ttgatctatt tgatgaaggg
     3181 ttgcttgatt ctttagcatc tgttcaatta ttagtcgagc tagacggaga gctggatatc
     3241 caagtccctg tatctgaatt cgagcgagag gattggagca caccgaataa aatcattcaa
     3301 caagcaactg cattgaaggg ttgagtatga aaaagaaact ttttgctatt ttcggcccgg
     3361 ttgttttagc tgttggctta ctgtttgtgt tttttacctc ctcctatcaa gtagatatct
     3421 ctaatccaga aacgattaga cgtgcctctt cttccatgtc agtgaacgtg ttgaaaggag
     3481 atgcgataaa aaattatgca ttatctgaaa aacaatatat tccttttttt ggctcaagtg
     3541 aattaagcag aatcagtcca tttcatccgt ctgttctggc agaaaaatac cagcgaaact
     3601 atcgtccatt tttgctggga gcacccggga cacaatcgtt aagccaatat atgatgatgc
     3661 gttctgcagg agatgctatg aaaaacaaga aagtcgtctt tattatttct cctcagtggt
     3721 tcgttaaaaa tggtgtaaaa actgattact ttaatacgta ttattctgaa cttcaaacat
     3781 acgactggtt gttttcaatg aagaaagtaa cgcctgctga tcgctattta gctagaagat
     3841 tattaacttt ctcaaaagta aaagagaatg atacgttgac ggcgattctg caaacgatca
     3901 aaaaagggaa gctaccttta cctgaatctt tgaatcagct gcgcagccaa tggaacatgc
     3961 tgaagagaga ggatgaagta ttcagcaata ttgggttgaa agatcgacag caaaaaatcg
     4021 atcatgagtc aaaacgatta ccaaaacaat accaagaaac agaattatcc attttagcaa
     4081 atcaaattgg agaacgagaa acgacgaata atccattcgg gttgaaaaac gatttttata
     4141 cgcatcgtat ccgagcccac gagccagagc tcaaacagtc acaaaaaaat tgggattatc
     4201 gtttttcgcc agaattttct gattttcagc tggtcttgga tcaattagca aaaaaccata
     4261 acgaagtatt gttcatcatc ccaccggtta acgagaaatg gagcgattat accggactat
     4321 ctcaagaaat gttacaagga tttgccaaaa agatcaaatt tcaattaaat agccaaggat
     4381 tcaatcgaat agctgatttt gtcaatcaag cgggtaccaa ctattttatg gaagacacga
     4441 tccatctagg ctggaaaggc tggttggctg cagatcaaca gatcagaccg tttttagaag
     4501 aaaatcatat tactgctagt aagtatcatc ttgacgatgc tttttttagc aaatcttggc
     4561 aacatcaaat accagataaa ctacaattaa aataaagaaa acaggcgagt gttgctcgct
     4621 tgttttcttt atttttgaat attctgaata aaagataaaa ataccaagtt tgcctgaaca
     4681 aaaatcataa gcaatggtat aatcacaata tgaatataga aatgggggaa aacatttgga
     4741 tacaaaagaa gaacagctac ttattgattt aacttctgca aaaggagtac ctggaaatga
     4801 agaagaggta agagaagtat tcagagaata tgccaaacca ttcgctgatg atatttttta
     4861 tgacggttta ggcagtgtga ttgccaaaca tggtgctggc ggaggcccca aggtctttat
     4921 ttctggacat atggacgaag ttggttttat ggtaacaaaa ataaccgaaa aaggattctt
     4981 ggaattccaa actcttggag gttggtgggg gcaagtcatg cttgctcagc aagttgaaat
     5041 caaaacacgt gaagggaaaa tcgtccacgg tgtgattggt tccaaaccac cacatgtttt
     5101 aacacctcaa gtaagaaata aaccttatga aataaaggat atgttcattg atattggcgc
     5161 atcaagccaa gaagaagcca aagagtgggg catacgtcct ggagatatgg tcactccata
     5221 tattgaatac aaacgaatga atggcagtaa atatttactg gcaaaagcat gggataatcg
     5281 tatcgggaca gctgtttctc tgcgagtatt agagaatcta tctaaagaag cacacccaaa
     5341 tgttttattc gcaggaagcg atgtgcaaga agaagtaggc ttgcgaggtg ctcgaacaag
     5401 tacacacctt gtaaatcccg atattgcttt tgctttagat actggtacag caggagacac
     5461 tccaggaatg acaccaaaag aagcagattc gattttagga aaaggcccac agattttgat
     5521 ttttgatgca tctatgatcc cgcataaaaa attattgaat tttgttattg atgtagcaga
     5581 ggaaatggat attccattcc aatatacagt gatttctggc ggaggaactg atgcaggaca
     5641 aatgcatttg acaagagatg gtgttccgtc tgtagccatt accgttcctg ttcgttatct
     5701 gcattcccat acatcagtga ttcatgaaga tgattatttg aatacagtga aactagtcac
     5761 agaattagtg aaacggcttg atagtgaaac agttgaaaaa attcggagtt atgaataatg
     5821 aataatagaa tacctatttc tgaatatcgg aaaaaagcac gagtttcttt ggaaaatcaa
     5881 tggggaatca atacttggct tgttttctta tctataatat ttacgagcgt catccagaac
     5941 ttttttgacg gtttcagtcc ttttccggaa gaaagcatgc agcagcactt attgagtttt
     6001 ctagtcgata atttactttt attcgcattc aactatgcat tgttttacat tgcgctagta
     6061 gttgtacggg gtggcagacc aaaaagtaac cttttatttt cagttttcca aagaaagtat
     6121 tttggttcga tacttggcat caatttgatc aattcagtca tcaactggct actcaacagt
     6181 ttaatctttc tgcccatgtt tttaatcagc ggagtgagta cttatatgca gctgcttttc
     6241 agttcaaatg cggtacctac gggtgtcatg ccagaagatt ttcttgatat cagctttgtt
     6301 ttatcgatga tagggatgat ttttgtttca gcactcatca tgagtattgt tggcggttta
     6361 ttccaatttg ctgcatggac aaagttcgac tatcctgatt tgtcggttat tcaatgtttg
     6421 aaatatggct ggtttttatt gaaagataga attggtacgt atattttgtt gcagctgtct
     6481 tttatcggct ggtatttgct aggagcaatc gctttgttca tcggtctttt gtgggtcatt
     6541 gtttatgtga atgtgtcagt tgcagaattt tatgaacaag cacggattga aaaaggaggt
     6601 ccagacgcct attttactta gcatgtaagc agtttattca tcaaaaaaat aacttttgtg
     6661 gtaggataaa tctttgaggg ggaatgtacg atgaaattaa ctgttttagg atgcttagga
     6721 gcgtatcctt ataaaaatca agggacaaca ggttatttgc ttcaatctga cgggttcaat
     6781 ttgttgatcg atgcaggtag tgctacgctg atcaaattac aagaacacct tgatccactt
     6841 gatctagatg ctgtgatcct tagccattat catcatgatc atattgctga ccttggtgta
     6901 ttacagtatt actggcaact tcatcctaag agaaatccgg agacattgct ttcaatttat
     6961 ggacacacaa aagattccat tcactttgaa gaattgacgt tggaaaatgt ctcaaaagga
     7021 catccttatt ttgaagctga agaattaaaa attggtcctt ttctgattac ttttatggaa
     7081 acaatccatc cagtagtatg ttacgccatg cgtatcgtag aagaaacaac ggggaaagta
     7141 ttcgtattta caggtgattc aggttatttg gaaagtttcg ctgattttgc gaaagatgct
     7201 gatttgtttc tagcagatac ctatcttttt gaaggaaatg aacatcatca tgcccatttc
     7261 acttcaaaag aagctggaga acttgcgaaa tcagctaacg taaagaaact tgtacttaca
     7321 cacctacctc agtttggtga tttagaacaa ttgaaaaatg aagcgcaaaa ggcagcaggc
     7381 aaagggattg atatagaact tgctgcagtt gacaaagtgt ttaacattta ggaggaagaa
     7441 catgattttt gtaccaaatg aaaaccacga cccaagaatc aatttagcaa ttgaacaata
     7501 tttgctgcaa gaaatgcctt tagatgaacc tattttgctc ttttatatca atgaaccttc
     7561 aatcatcatc ggcagaaacc aaaatacgct tgaagaaatc aatcgagatt atgtggaaga
     7621 aaatggcatt catgttgtcc gtcgcttaag tggaggcgga gctgtctacc atgattttgg
     7681 aaacctgaac tttagtttta tcatgcctga tgatggggat tcattccgtg actttgcgaa
     7741 agtaacacag ccaatcatcc aagcacttca tgaattgggt gttgctgggg cggaattgaa
     7801 aggccgaaat gatctagtca ttgatggcat gaaattttct ggcaacgcga tgtatgctac
     7861 caacggtcgg atgtttgctc atggaacaat tatgttcgac tctgatatca atgaagtagt
     7921 caatgcatta aaagtaaaaa aagacaaaat cgaatcaaaa ggaatcaaat cgatccgttc
     7981 tcgtgtaacg aatatcaaac catttcttcc agaagaaaag caaaaaatga caacagaaga
     8041 atttcgtcaa gaaatcttgt tgaaaatttt cggcgtagag tcagtagaag aagtaaaaac
     8101 gtacgaattg actgatgaag attggaaaaa aatcaatcga atctctgagg aatattatcg
     8161 aaattgggat tggaattacg ggaagtctcc agatttcaat ttctctcgcc aaaaacgttt
     8221 ttctatcggt tcgattgaag tccatttgaa tgtctcagaa ggaatgatca aagaagcaaa
     8281 aatctttggc gatttcttcg gactaggaga tatctctgac gtggaaaaaa aattggttgg
     8341 acagaaatat gataaagcat ctctatcaaa aatagtcagc caaattgatg tgaagaaata
     8401 ttttggtgcg atagaaccag aagaattgct taccttactt tattgaaaat aaaaaatagc
     8461 ggaatccaca tatatggatt ccgctatttt ttatacagga ttcgggtaat ttcgagagag
     8521 ttgaacagtg atccgatcaa tgaaagtatc tttttcctgc gttagtgttc ctgtgtaacg
     8581 atccgctacg tctttcatca tcattaagtc aaaacgttcc tgatcatcta attcttctgt
     8641 gatgcctcga ctaaataggt ttgtatcgta atgtgcacta tgttcgcaac aaatgacgaa
     8701 atgttcctct tgttcgtgta tcgacaaata aatttttcga tacagtggat tttcgatggc
     8761 atgagtttct ctaaaggaat gctctaaagc gtggatcaaa agttgagcca gatcgtcatc
     8821 gttgatagga agttcttcgg gaagatctgc ttggaaggtt atctgaatat tccgtttggc
     8881 agctaacttc tggtaacgcg ctaagataag atttgtcacc tggtggttgc aaaaagaaca
     8941 cagcatttcg aaattttttg cttcttctaa tatcttttcc acatagtttt tagcttttgt
     9001 ttggtccgta tgcattaaat cttgcagatt ctccatctct tggacaaatt ccaggcgaag
     9061 tgtatttact tcttggatat gctgtcgaag ctgctcataa tgttgctcca acaaatcatt
     9121 tttgattttt aagaaatcta cttgtctccg atctttgtcg gtatttaaga tgtgatcgat
     9181 tagattgtag ctgataataa cgattagaat gataatatat aaaatcgtag accaattgat
     9241 cgttgtttga tagataccta atgccgtttg gatgtaaatc aagcaatgga taatggctac
     9301 caagacgatc caaggtgtac aagctacata aaaacggtta ttcttatatg cttcgccaat
     9361 gcatgcaagt gaagtgacta aagtgcttat aacactagca atattagcaa tcggcattaa
     9421 ttcaggaagt tctgcaggag taaaagttgc ataaagcaga acgccgataa aaatcaaaag
     9481 ttggaacgta caaaaaattc cgtaccattt tttatagtaa aacattcggc tccaatagta
     9541 gcagatgatg aaagctgacg ttaagataat aaataaatga gataaaaaag aatgaacgaa
     9601 tggttcaaaa agaataccac ttaaaatatc ttctgataca gcttcaaatc ctaatgccaa
     9661 agcaaagcaa cttaagagaa taagtgagta ttcaagacgt ttttgtttat acatcaagta
     9721 gatcgtatag aaaaaggtcc ctatagcgat aaaaacagcg attatcatcg tcaatacttg
     9781 cggcactgat tgtgaataaa taaaactaat caatggtcca gttgttccta gataaacttt
     9841 cggtggcagt ccagcatagt tctgataagg agatgagact gtaagggtca atttttttcc
     9901 gatataattt gctggtaaat ctatcacagc caatgagagt ccgggattgc tttcttcctg
     9961 ataatctttc cgttggtata aaatcttttc gcctagatat acagtaatcc attgatggtt
    10021 tcctttgatt agtaaatttg cttcgtccaa cggtttttca agtgtcgttt ccataatgaa
    10081 tgtttcgtct ttatccacgg taggaatcgt acgagtgtac ttgctgtcaa acttaaaatc
    10141 aggatcatct tttgtataga aaacccacga atcttttatt tctttcattt gattgctgtc
    10201 cgcaggagaa aaaataaaat aaaagaaata agcaattcct gttaaaaata acaatgataa
    10261 tataatgatt atgctccact
//
LOCUS       DARBFO010000087        10193 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000087 DARBFO010000000
VERSION     DARBFO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.088"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            120..626
                     /locus_tag="SCK51_002345"
     CDS             120..626
                     /locus_tag="SCK51_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEG1252930.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="SCK51_002346"
     CDS             643..1542
                     /locus_tag="SCK51_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEG1252931.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2513
                     /locus_tag="SCK51_002347"
     CDS             1629..2513
                     /locus_tag="SCK51_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEG1252932.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2705..2776)
                     /locus_tag="SCK51_002348"
     tRNA            complement(2705..2776)
                     /locus_tag="SCK51_002348"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2741..2743),aa:Arg,seq:ccg)
     gene            2836..4170
                     /gene="rpoN"
                     /locus_tag="SCK51_002349"
     CDS             2836..4170
                     /gene="rpoN"
                     /locus_tag="SCK51_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEG1252933.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4319..5410
                     /locus_tag="SCK51_002350"
     CDS             4319..5410
                     /locus_tag="SCK51_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEG1252934.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5451..6452
                     /gene="gap"
                     /locus_tag="SCK51_002351"
     CDS             5451..6452
                     /gene="gap"
                     /locus_tag="SCK51_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEG1252935.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6562..7752
                     /locus_tag="SCK51_002352"
     CDS             6562..7752
                     /locus_tag="SCK51_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEG1252936.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7856..8611
                     /locus_tag="SCK51_002353"
     CDS             7856..8611
                     /locus_tag="SCK51_002353"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEG1252937.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8727..10025
                     /gene="eno"
                     /locus_tag="SCK51_002354"
     CDS             8727..10025
                     /gene="eno"
                     /locus_tag="SCK51_002354"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEG1252938.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2112 g         2630 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
     2641 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
     2701 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
     2761 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
     2821 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
     2881 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
     2941 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
     3001 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
     3061 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
     3121 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
     3181 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
     3241 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
     3301 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
     3361 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
     3421 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
     3481 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
     3541 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
     3601 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
     3661 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
     3721 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
     3781 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
     3841 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
     3901 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
     3961 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
     4021 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
     4081 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
     4141 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
     4201 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
     4261 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
     4321 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgctgga
     4381 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
     4441 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
     4501 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
     4561 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
     4621 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
     4681 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
     4741 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
     4801 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
     4861 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
     4921 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
     4981 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
     5041 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
     5101 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
     5161 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
     5221 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
     5281 catggcaatc gcgggcggaa aatcaaaagc caaagcaatt cgagcttata tgaaaaatgc
     5341 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
     5401 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
     5461 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
     5521 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
     5581 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
     5641 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
     5701 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
     5761 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
     5821 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
     5881 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
     5941 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
     6001 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
     6061 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
     6121 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
     6181 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
     6241 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
     6301 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
     6361 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
     6421 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
     6481 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
     6541 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac
     6601 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
     6661 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
     6721 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
     6781 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
     6841 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
     6901 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
     6961 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
     7021 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
     7081 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
     7141 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
     7201 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
     7261 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
     7321 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
     7381 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
     7441 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
     7501 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
     7561 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
     7621 gactcagccg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
     7681 ggtggcggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
     7741 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgatca aaaggtgaag
     7801 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
     7861 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
     7921 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
     7981 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
     8041 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
     8101 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
     8161 ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct atctttgcaa atggtatgac
     8221 accaatccta tgctgtggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
     8281 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacaag tgtcaaacat
     8341 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
     8401 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
     8461 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
     8521 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
     8581 attcttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
     8641 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
     8701 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
     8761 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
     8821 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
     8881 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
     8941 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
     9001 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
     9061 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
     9121 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
     9181 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
     9241 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
     9301 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
     9361 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
     9421 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
     9481 aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga aaaaacaact gacgaaatga
     9541 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gacggattag
     9601 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
     9661 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
     9721 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
     9781 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
     9841 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaacgctggc caaatcaaaa
     9901 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
     9961 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    10021 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    10081 cttagttaag taatcgaact ctctacttgc tgaaatatcg gctctttgtc aataaggact
    10141 gatgagttgt gcaaaatcaa atctgagtca gaatgaacca cattctggct cag
//
LOCUS       DARBFO010000088        10107 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000088 DARBFO010000000
VERSION     DARBFO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.089"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            219..1454
                     /locus_tag="SCK51_002355"
     CDS             219..1454
                     /locus_tag="SCK51_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEG1252939.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(2453..2713)
                     /locus_tag="SCK51_002356"
     CDS             complement(2453..2713)
                     /locus_tag="SCK51_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEG1252940.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2740..4041)
                     /locus_tag="SCK51_002357"
     CDS             complement(2740..4041)
                     /locus_tag="SCK51_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1252941.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(4333..4791)
                     /locus_tag="SCK51_002358"
     CDS             complement(4333..4791)
                     /locus_tag="SCK51_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1252942.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(4804..6135)
                     /locus_tag="SCK51_002359"
     CDS             complement(4804..6135)
                     /locus_tag="SCK51_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HEG1252943.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(6146..7588)
                     /locus_tag="SCK51_002360"
     CDS             complement(6146..7588)
                     /locus_tag="SCK51_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1252944.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(7575..8339)
                     /locus_tag="SCK51_002361"
     CDS             complement(7575..8339)
                     /locus_tag="SCK51_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEG1252945.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(8443..9318)
                     /locus_tag="SCK51_002362"
     CDS             complement(8443..9318)
                     /locus_tag="SCK51_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1252946.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(9573..9657)
                     /locus_tag="SCK51_002363"
     tRNA            complement(9573..9657)
                     /locus_tag="SCK51_002363"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9621..9623),aa:Leu,seq:caa)
     gene            complement(9675..9746)
                     /locus_tag="SCK51_002364"
     tRNA            complement(9675..9746)
                     /locus_tag="SCK51_002364"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9712..9714),aa:Gln,seq:ttg)
     gene            complement(9756..9828)
                     /locus_tag="SCK51_002365"
     tRNA            complement(9756..9828)
                     /locus_tag="SCK51_002365"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9793..9795),aa:His,seq:gtg)
     gene            complement(9890..9974)
                     /locus_tag="SCK51_002366"
     tRNA            complement(9890..9974)
                     /locus_tag="SCK51_002366"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9938..9940),aa:Leu,seq:caa)
     gene            complement(9995..10065)
                     /locus_tag="SCK51_002367"
     tRNA            complement(9995..10065)
                     /locus_tag="SCK51_002367"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10031..10033),aa:Cys,seq:gca)
BASE COUNT         3264 a         2123 c         1570 g         3150 t
ORIGIN      
        1 gtatttatct ggcacaacat ataaatcgat caaaaaattg ttgcgtgttt ttttgaaaat
       61 tggagtttca acttgattac aaaatttaaa tttagaagtt gtgaaaacag cgagagacag
      121 tttaatattc aaaatataca taggctactc gaacgctgtg ttttttagta tactagtaag
      181 aaacgaaggg atagactcat tccaataagg aggatttcat gaagacaatt ctttttgctc
      241 ctgcagtatt caatttagct gagacaacac gaatgattga ggttgccaaa gctatgaaag
      301 accgttatta ctgtgtattc tttggctttt ctaaaacttt tgctcctttg atcacagaaa
      361 gtggctttga gtatcattta ctagcgccga tccttacgaa aaaacaagaa gaacaaatca
      421 taaaatttga ccagttgcgt acacttaaaa acccttttac caaagaaatg gttgcctctc
      481 gtatcactag tgaacagagt ctgattgcta agtttgagcc tcgactgatt gtgacaggtt
      541 cgaatgtgac aattttttta tcggcccgca ataaaaagat tcctttagta tatgttaaac
      601 cctatgcttt gagtgaacct ttttttcaat cagagcatgc gctgatgccc cacgagttac
      661 gtttcttcgg ccctttaagc aatccccttt ggcactggac aaaaaagcgt atagtaaata
      721 cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa agaaatgctt cctttaccaa
      781 aaacaagttt acaaatgatg gatgctgatc taaatctcat cacaacgcct aaactatttt
      841 tggaagaaga acagctgccg gaaaattatc gaatcgttgg tccaattttt gctaagctca
      901 gtacttcctt gcctgaagaa gtgctaactt ttatacagcg aaagcaacaa gaaagaaaac
      961 gtattatcta tttcgcaatg ggtagttcag gcaatccgcg tatgttacgc aaaattttgt
     1021 tcttcttaca aaaacaaccg gatctagcgg ttattgcacc tattgactat ctgcttgatg
     1081 acattcaatc tgactcagaa gatcttttaa taacagaata tctgtctagt catcttttgc
     1141 atgaattcat tgatttttct tttatccacg gcggggaagg gaccgtccaa actgcctgtg
     1201 aatccggcaa accgtttatc ggtgtgggga tgcaatttga gcaaagctgt aatattgact
     1261 attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc agtgactgaa agaaaactgc
     1321 agattgctct gcgcgaagtt tcttcaccca agattcgtca gcaggcacgc acgctgcaag
     1381 ccgtttttcc aataaatggt ccacaacaag caattcaaga aatcgaaacc ttcctgagca
     1441 accacccaat atagtttcaa gtagaaaaag cttttttaat agagactcat cagtaatttc
     1501 gctattcgag acccagacag tgaaaataca aaaaaagtaa atagtggatt gaaaagttag
     1561 agttacttaa attacggtgt ctaaaaaaca caaaatcatc cttaaaataa aagatctgac
     1621 gctatccaaa tgagcatagg tagcgtctgc catttattct tagtcaaatt ctttgacacc
     1681 tttctctggt tggatcatct caacgtttct tcaaaagaaa gaccataata attaggcaaa
     1741 tacattttct taaaatgtgt catatagatc tagaagcatc gctttgtaga gatttgtgcc
     1801 gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt accactttat ctttgataat
     1861 caacctccct cttctgttga gacaatgatc tcgcaatccg atgcatactc ctatataaac
     1921 tgccagcaat ccccacatat tgagtagatc atgtcagaat gttctatgtc gtcagcaacg
     1981 atacactaaa agtcacgttc accatgactg attgctattc ccagcacaat tatttctata
     2041 cacgtttcat gatttgaata aacactcaca tcatacatat taccattcat acaccatact
     2101 gcagccccta ctatatggtt gtaagctccc aaatctatat ttgacttgat cactttggta
     2161 gtagatctac taattctaaa tccattgctg ataaaatttc cattttttcc tccttctttc
     2221 tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa agggctgtga caaaagcctt
     2281 gtcacagtcc catattccga ataaacggtg gtcaaaagct tgcatctgcg gtaataagtt
     2341 caaccaaacg aaaaataagc caacatttca caaaaattta agaagcaatt ttcgtaaatt
     2401 tcttcttatt tcttgaagct aaccacttct gttacaacca cttttagaac atttaaagct
     2461 tcgatgtgac aaaccatcta tgctgctcga tcataaaatt gatagcaaaa attccacaaa
     2521 gtacatatgt cccccatgaa aacaaaacat tcatatgcgg tacaatgatc tttggaagaa
     2581 tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt gatttgaata tgatatgtag
     2641 ctagcatatt caatataagg tgactagccg ttaataaaaa gataaaaaca aaaaataaaa
     2701 tcagccaata cattgtttcc caccttctaa aaaccaaact tagacagtct ggacttcgtt
     2761 ttctccacga ctttgttcat ctgacagcat ctttttatca taagctttta caaatggata
     2821 atagataata aatgctatca atgtgttgat catgatcaag ataacaacac gccagtcatt
     2881 tcctgctgaa agataaccgc cgattggagc tggtaatgtc catgttggca aaacagtaaa
     2941 tctattgacc atttctaatt tgactgcaaa ataactgata atagtcaaaa ctaaaggaac
     3001 taagttgaac gggatgaaca tgtaaggatt caacatcata ggagctccaa aaattatggg
     3061 ctcattgata ttgaagatac caggaataat agaaaaacga ccaacctctt tcaagaattt
     3121 tgatttacag aagaatataa acaaaacaga taccacgatc gttgctcctg caccaccaat
     3181 tgttaccgtc cattgataaa actgttcagg agcgatatag ggtaattggg tggcagctgt
     3241 accatctgcc aatgcctttg cattctcatc caacatgatc agccacattg gacgaacaac
     3301 agcccctaca atactcatgc catggatacc aaatgaccac aacaaatgga tcaaaaacat
     3361 tggtagtaaa acaccaagca aattattccc taaaatattg gttaaaggct cgaataacga
     3421 taataaggca gcgtttacat caaagcccgc gacgcttcga atcagccaag taattgttaa
     3481 aatgactgca cctggaatca atgcctcaaa actacgggca acagcaggtg gcacttgttc
     3541 tggcattttg atagtaatat ttcgattttt aaagaaacga tatacttcca ctgcaaaaat
     3601 catagataag atccccgcaa acatcccaga tgctcccaaa gaactcatag gtaagaccca
     3661 gccaagaggt gcctcttcag gagttaaaac gtttacagga acttgtaaca taaagaacgt
     3721 tcctaaagat aatataccac ctgtcacact atctaatcca tatgatttag ctagatccga
     3781 acccataaca aaagttgcat aaatagccat caatcccatc gtcatcctaa agggtaaaac
     3841 aatcgttgcc gaataagggg caataaattc attccatcca ggaaacggta aattgataaa
     3901 gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt gaaacaaccc cattacgaat
     3961 tgctaataaa tgacgctgtg taccaatttt tgccattggt gggacaagtt tctcttcgac
     4021 ccatttcatc agcttttcca ctaaaaccac tccttttcaa taaaatatga accattcatt
     4081 gcatgacaac gcaaagaatg ttcataacta attatataat taagaaattt tatttcaata
     4141 tattctctta aataatcaaa tttattttta agaatcctga aaataagttt tctaaatcat
     4201 aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa tgattaaatc tagtgattga
     4261 aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa atatcaccct atttaacttt
     4321 tttcatcgta cactaaaatt caaaacaact atctttaatc tttacttctt ggttttgttt
     4381 taataaattt ccttcaaaaa tgcctggaga aactacaatg atcaccgtat ctttttgtgc
     4441 ttgctccaac ttcttcaaat ctataatagc taacagtgtc ccttctttta cctcctggtt
     4501 actttcaacc cgtatctgaa acggacttcc ttcgagttca actgtatcta aacccatatg
     4561 gatcagtatt tttctgccag attcactttc gatcgttatg gcatgtcggg taggaaatat
     4621 attatcgatt attcctttaa ctggcgcatg gatatatcca tcatgattcg ttatagcgta
     4681 tccaactcct atcatttttg aagaaaagac ctcgtctttt actgctgtta atggaataat
     4741 ttgaccacta gctacagctt ttagaatgtt tcttgtttta aagaaatgca ctttttatca
     4801 cctctacttt aatgctggcc aatatttttt gtttactttg atcatttcat ctagtatttt
     4861 ttttgcgact ctggcatttg gtacggtttg attcaatgta aatgcttgta aagctttttg
     4921 ataagaacct tcaaagtaag catctactag taacttctct actgcaactt gtgcctccat
     4981 caatccttta tgaaaatcac tgattggttt tcttagtgta attggctcca caccagttgc
     5041 accaacatat gccggtattt caacaactgc atctgaccgt agattcggaa tcgccccttg
     5101 attaggaacg attagcataa agcgacgacg atcatcattc aacaaggaaa tagctatatc
     5161 gacgatatat tgtccatgct ccccaaaatt aaagttcaat acttcttcca caataccgcg
     5221 gcgtatttta tcagccattt ctttggtatt tttctctcgt ccatccatga ccatattagc
     5281 acgtgtatac tctttgtcag tatgctcaac gaccatatca ggatataagt aatactctaa
     5341 gtaattattc gggatattac ttggaaaatt tttcgtgatg actgacatca tttgaaaggt
     5401 tttttgccaa gttttatctc ctatattaaa atcggcgact tgcatttctt ttgttattag
     5461 tttttcaata atctctggca taatttctcg ttgcaattct ttatcataaa tcgatgtata
     5521 ccagccaaag tggtttaaac cataatacgt aacgatccaa ttcttacgat cataaccgta
     5581 attcactgca atcgtttctt caattgaaat agtcatatca caggcattaa tcatttttac
     5641 attcggatat ttacgacgaa cagcttcaga aacgatcgac tctggatttg tataattcaa
     5701 aatccatgct ttgggggcat agtcttgtat atgatcgaca atttccaata aacctttgat
     5761 tgagcgcatc ccataagcaa atcctccgag tccacacgtt tcttgtccta ctaatccatg
     5821 tttaagtgga atcttttcgt ccttttctcg catctccaat cctccaacac gaatttgtga
     5881 gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca tccgttgcac gaatgcgaat
     5941 atttgaaaaa ccttttcttt tcagcataaa tttaacaatc aagtacatat ccatatttct
     6001 ttcttcatca atatcatata aacgtatttc agataatggc aaccgttttt cattgttcaa
     6061 aatggcttga acgattcctg gtgtatacgt actcccgcct ccagctatag tgataatctg
     6121 ttttttcatc atcatctttt cctttctatt cttctaataa tttattgaat tctgccataa
     6181 cataagtcac ttctaagccg atgataattt gataattttt ttcagttggt ttaatgatcc
     6241 cgcttatccc taattgcttg attcgctgtt cctcgaccat ggtgggatcc actacctcta
     6301 cccgtaggcg tgtattacaa ctatagacat ccaggatatt ggcttttcct ccaaacgctt
     6361 gtagcatttt tgttgccata tattcataat ttttacttgc taaccctaac tctgtttctt
     6421 ctaaggttac ttcattgcca aactctgcct tagcttcacg tcctaatgtc tgaatgtttc
     6481 tcttattgat caaataataa aaagtaacgt aatacaaggc aaaaaagaaa attccgattg
     6541 gaagaatcaa aatagcattg gtagcgattc gaaaattgat gagataatca acaatacatg
     6601 ctccgaatgt aaaaccaatt cgtatattga ataagtaaca gacaatccct gctaaaccgg
     6661 caaaaagtgc atggatcacg tacaacatag gagaaagaaa catgaaacta aattcaacag
     6721 gctcagtgat atttgcaata aatgaagttc ctgcaccgct tcccatgatt cctttgacca
     6781 tctctttatt ttctttataa gctgctttat agatagctag acatatagct ggtataccaa
     6841 acatcattac tacaaaaaaa actgataaaa acagtccagc ctgaggatct ccgttgataa
     6901 agcgtgtcat ctctcctctg aaaacttcct tgctaggaga tgtatagctt cctaaatcgt
     6961 aataaatata cgtattcaac acatggtgca gtccaaaggg gatcagcaaa cgattcaaaa
     7021 acataaatat ccccacacct aatgctccca tatcaacgag cagttttgca aaattatcaa
     7081 tcccaatttg gatgagcggc cagataaagc ccaaaaaaac agctgcgatt gtttgtagaa
     7141 tcatcaccat agtcaatggg aatttttcgc ccgcaaaata agaaaaaact actggtaatt
     7201 tttgttcttt aaagcgatta tacgtccatg ccgctactaa accggaaata attccaccaa
     7261 aaatccccat attcacttct gggtccataa tttctaagcc ttttttcaag gtagcaatcg
     7321 ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt tttgacaaaa ccgattgcaa
     7381 ctcctatagc aaataagata tccaagtttc caaaaacggc atttccagct gcaccaatta
     7441 ctgggatgtt cagcatatcg tcagctgata aacgcatgat caatcccgct aatggcatcg
     7501 ctgcaactgg aagtagcatc gctcttccaa gcttctgcac atttgattta acattcattt
     7561 ttattcctcc tctattaaat atcctcatat gttactaact caatcaaagg atcatttttc
     7621 actttttgag caaatgctga atgaattaaa aattcgactt ggtgggtacg aggatacaca
     7681 aaacttgaac catttaacaa ttcctgatcc aaatacgctg cgtgacacat gacttcgatc
     7741 gttttatctt ctctcaatat catgagtaaa cttgccaaat attcttccac actcgtattg
     7801 atttgtcgtt ttttttcatc agtttctcct acgacatcga aacaaggctc aaaataatcg
     7861 acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa ccggcaaatc atattttttt
     7921 gctaatgtaa caactacctc ctgattttca tccaaagtat gagtatgatg atgactgtct
     7981 aaatgagttg gtatgatccc cgcatgataa attttttgaa tctgagcatt ccattcctga
     8041 tacagttgat ctttgtcaat gttaaatgga tgctgataat aagataagga tctgaactct
     8101 ccgctttcga taagagtatc gacagttttg aggagtggtt tgccacacgt caatgttaaa
     8161 tggactccta cacctagcct aggcatttcc tttcttagtt tcacagcatg ctcgaaaccc
     8221 ggcatatttg ccatcaatgt agttgatgtc aaaattcctc gctgataagc atccataatc
     8281 ccataattca caccatggct atagccaaaa tcatccgaat taaaaatcac ttttcccatt
     8341 tcattctcct tcttttttct actaaaatag gcgcaaccaa ataatcatag tccatttact
     8401 atgtgtcatt tggttgcgcc tatagatagt tacgccctta ttttaataga ttcttgctag
     8461 atgaattgcc aaatagatga tttcattttc ttgaatggca agttgaaatt gctgttcgat
     8521 gatatcttta atctttagtg ctgtacaata cgctttgcta tgtttaaatg tttcagtaaa
     8581 aaaaccaata ttttctactg gcgtttcttc ctgataaatc aagcgtttaa ccaggaattt
     8641 gatatgaacc attaagcgat gataagtcag tgattcttta tcataaacgt ttgaatagga
     8701 tttttctaga tgggacaaaa tcgtggtcgt catttctact atttttaagc tctttaaact
     8761 attaggctct cctagctcag cattaatgat atgcatagtg atgaatccta cttcatttga
     8821 ccctaactcg gtttgatatc gatgattgat tcgctcgacc acttctactg ctatatcata
     8881 ttcatttgga taaaggaatc tcatttctgg caacataatt gctgtgacat ctctatcttc
     8941 tctttttcgt tgaatagcaa aataaatatg atctgtcaat gtaatataga tatttgaatc
     9001 taatggagag gagatcctag ctgttatcag ttcaatcact tcttccgcaa aatccataaa
     9061 ttcgattgga atctgcgaga ctatctcact gaatcgtttg atcttttctt tagaatctaa
     9121 tacaaataat ttgtcagcat cctcaacatc aaatggctta ctcactttag cagaaaagcc
     9181 aatccctttc cccatcgcaa ttttttctat tccatcgtca tcgatcaata cagcattatg
     9241 attcagcttt ttcatcacat acaccgttaa ttcacctcct ctatatgata aaacaaaaca
     9301 acttgcagac tccttcatac atatttttgt ctccaaacaa tttttaaaga caaaaaacga
     9361 cctaagaaag aaccctttct taggtcgtgc ctaattgaat agtagcaccc tacataattt
     9421 acaaaaatag tttaacacat aataaaagcg ttatcaatat cttttttact cgttttatga
     9481 taatacttgt atcgaattga tttggaggta attacataaa aacaaaaaaa atatagcaac
     9541 tctctataag aaagttgcta tattgataat tataccggcg gccggggtcg aaccggcacg
     9601 tcctcaacgg acactggatt ttgagtccag cgcgtctgcc aattccgcca cgccggcaaa
     9661 tttaaaaata ataactgggg tagctggatt cgaaccaacg catgagggag tcaaagtccc
     9721 ttgccttacc gcttggctat accccaagaa taaagggcga atgatgggaa tcgaacccac
     9781 gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc acattcgcca tcataaaaga
     9841 tgttatttaa tttctcaata aaaaactaag atgaagacat cttagtcaat accggcggcc
     9901 ggggtcgaac cggcacgccc tcaacgggca ctggattttg agtccagcgc gtctgccaat
     9961 tccgccacgc cggcaagttg atttattagt actaaggcgg taaccggatt tgaaccggtg
    10021 atgaaggttt tgcagacctc tgccttacca cttggctata ccgccattat actattatca
    10081 atctataaaa taaagaataa attatag
//
LOCUS       DARBFO010000089        10090 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000089 DARBFO010000000
VERSION     DARBFO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.090"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            162..392
                     /locus_tag="SCK51_002368"
     CDS             162..392
                     /locus_tag="SCK51_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEG1252947.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="SCK51_002369"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="SCK51_002369"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEG1252948.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="SCK51_002370"
     CDS             1803..1979
                     /locus_tag="SCK51_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEG1252949.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="SCK51_002371"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="SCK51_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEG1252950.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="SCK51_002372"
     CDS             2474..2941
                     /locus_tag="SCK51_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1252951.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="SCK51_002373"
     CDS             3026..3910
                     /locus_tag="SCK51_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1252952.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4024..4260
                     /locus_tag="SCK51_002374"
     CDS             4024..4260
                     /locus_tag="SCK51_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEG1252953.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="SCK51_002375"
     tRNA            4348..4428
                     /locus_tag="SCK51_002375"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="SCK51_002376"
     tRNA            4435..4506
                     /locus_tag="SCK51_002376"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            4631..4801
                     /locus_tag="SCK51_002377"
     CDS             4631..4801
                     /locus_tag="SCK51_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252954.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5021..5728
                     /locus_tag="SCK51_002378"
     CDS             5021..5728
                     /locus_tag="SCK51_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1252955.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="SCK51_002379"
     CDS             complement(5788..6432)
                     /locus_tag="SCK51_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEG1252956.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="SCK51_002380"
     CDS             6559..6846
                     /locus_tag="SCK51_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252957.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="SCK51_002381"
     CDS             6954..7274
                     /locus_tag="SCK51_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1252958.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="SCK51_002382"
     CDS             7355..8668
                     /locus_tag="SCK51_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG1252959.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="SCK51_002383"
     CDS             8665..9384
                     /locus_tag="SCK51_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEG1252960.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="SCK51_002384"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="SCK51_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEG1252961.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3363 a         1669 c         2152 g         2906 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaacgaaaa
//
LOCUS       DARBFO010000090        10086 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000090 DARBFO010000000
VERSION     DARBFO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.091"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(247..759)
                     /locus_tag="SCK51_002385"
     CDS             complement(247..759)
                     /locus_tag="SCK51_002385"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEG1252962.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="SCK51_002386"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="SCK51_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEG1252963.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="SCK51_002387"
     CDS             complement(3212..3655)
                     /locus_tag="SCK51_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEG1252964.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="SCK51_002388"
     CDS             complement(3656..4444)
                     /locus_tag="SCK51_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1252965.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="SCK51_002389"
     CDS             complement(4441..5382)
                     /locus_tag="SCK51_002389"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEG1252966.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="SCK51_002390"
     CDS             complement(5559..6146)
                     /locus_tag="SCK51_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEG1252967.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="SCK51_002391"
     CDS             complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="SCK51_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEG1252968.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="SCK51_002392"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="SCK51_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEG1252969.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="SCK51_002393"
     CDS             complement(9063..9998)
                     /locus_tag="SCK51_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1252970.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DARBFO010000091        10074 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000091 DARBFO010000000
VERSION     DARBFO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.092"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(538..762)
                     /locus_tag="SCK51_002394"
     CDS             complement(538..762)
                     /locus_tag="SCK51_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252971.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            953..3730
                     /locus_tag="SCK51_002395"
     CDS             953..3730
                     /locus_tag="SCK51_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1252972.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3811..4230
                     /locus_tag="SCK51_002396"
     CDS             3811..4230
                     /locus_tag="SCK51_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEG1252973.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4242..4712
                     /locus_tag="SCK51_002397"
     CDS             4242..4712
                     /locus_tag="SCK51_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1252974.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4729..5508
                     /locus_tag="SCK51_002398"
     CDS             4729..5508
                     /locus_tag="SCK51_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1252975.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5495..6316
                     /locus_tag="SCK51_002399"
     CDS             5495..6316
                     /locus_tag="SCK51_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1252976.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6333..7382
                     /locus_tag="SCK51_002400"
     CDS             6333..7382
                     /locus_tag="SCK51_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1252977.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7395..8399
                     /locus_tag="SCK51_002401"
     CDS             7395..8399
                     /locus_tag="SCK51_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1252978.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            9032..10051
                     /locus_tag="SCK51_002402"
     CDS             9032..10051
                     /locus_tag="SCK51_002402"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEG1252979.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3383 a         1656 c         2098 g         2937 t
ORIGIN      
        1 aaaaaatata tcaagtaaat gtttatagat gaaactctat ttacttcttt tttaatttgt
       61 tcttgcaatc ttttttgcta cgtaattttt gtaaaaaagt gggttgagag aaatctaatt
      121 gattttcaaa gaaggagtat gaaaatgaga aaaatattta ttcgtttatt gtgggtacta
      181 tcttagagga ataaataata gtggagaaaa ctatggtttg gcgcagattc tatcaggttt
      241 gatggaacga agacaacctc ccgacatcaa gtcaaaagct acttgagatt ttgatctgaa
      301 aataaaaaaa gaaaacaagg taggaacatc aatgtttcta ccttgttttc gtaatcaatg
      361 ggaaaaaact aagagtggtg tacgtaatca ataaaaagga tggtgacaac agtacctctt
      421 tactctctga aagcatcata actcgttttt cctcattatg ccaataaatt gttgttatac
      481 cacatataag gatttgtaat agctaaaaac agacaggaaa gttgaaatct gtgaatctta
      541 ttttgtatgg aaggatttat agaacgcaaa aatcaagata aagatgaata aaaatcctgt
      601 ttcggctata tattgcgtat ctacgccaat aaaacgagtt tgttctgtca tagccatata
      661 aagcaaaggc aaaaatacaa ctacagcgag ataagatccg atgatttctc gaaaagtttt
      721 ccaggaagta tgtttcctgt ttttatattt atccgatttc attgaatagt catagcctcc
      781 ttattctttc atatgttgaa attccagtat aaaaactgga aggaactgta cgttttttag
      841 aataaaaatt ataagggata agtaggctgc tgtctataat aagatatatt tttcaattct
      901 ttattaaaga ttttattgac aaaatgaatc cgttttctat gatggaggag gggtgaataa
      961 gttgttgaag aaggaaattt tatgttactt agagaaccag acagcctttt tcgatttaga
     1021 acatatgaac gaggtcttta cagcacaaag gttagctgat cattttgggg taaaaagaaa
     1081 cacagtcagt cattatttga accagttaaa tgatgagcaa ctactagtca aaatcaattc
     1141 tcgaccagtg atttatttcc acaaagaagc ttttgaaaaa caatttttta agttacgcac
     1201 gaatttttat ttttctatcg atgatctcaa agaggaacag ccttttttcg cgcaaccaaa
     1261 agacttattc tcttttatga tcggatacga cgccagtttg aaagaaagta tcgaacaaat
     1321 caaaacagct ctttactatc cagatggcgg attaccactt ttgattacag gcgaaagtgg
     1381 cacaggtaaa agttatttag ttaatttggt ctatcagtat tgtctgcttc atgatctgct
     1441 agaagattct gcaccattca tcacttttaa ttgtgcacaa tatgcagata atcctgaact
     1501 tttgactagc aatttgtttg gacatgttaa gggagcgtac actggtgcag aagaaaataa
     1561 aaaaggggct tttgaagcag cagatggtgg gatcttgttt ttagacgagg tacatcgctt
     1621 aacaccagaa ggtcaggaaa aactgttcac ctatcttgat caaggtgtga tttatcgtat
     1681 gggagaacct aatttgtcac gccggatcaa aacacgtctc ttttttgcta cgactgaaga
     1741 gattactagt catttcttaa caactttcat tcgaagaata cctatccgaa tcgaactacc
     1801 tgcacttaat cagcgtagtc gtaatgaacg gctagaactg atttactctt tttttctcga
     1861 agaacaaaga aaaatgggga gccctttgtc agtcagtgga ccagctttgt cacttttaac
     1921 aagtcagaaa ttatccggaa acataggaga gctgaaaaat atcgtcaaag tttcagcagc
     1981 aaaagcatat gctgaacagc gtgaacagcc tgaaatccat gtcacgatcc atcatttgca
     2041 aaaagaacta cttgcgcagc ctattactaa aaatacagca cagcaaggaa tctatattac
     2101 gcaagaccag accttagagc aattaatgga gaagcaacag cccgaacagc agcgaatcgt
     2161 taaaagtttt gagcaaatcc taattgattt caagaaaaat aactgtctgc tatattcttg
     2221 cgaaggaaaa ttaaagcaag aagtgattca attatttgat tttttgctgt ttgagaccag
     2281 tagacaagaa aaacacgagc tgctggttta ttggacacaa tctatccgag agacctttcg
     2341 acaaatggaa actgcctatc aaatcaaatt cgatggaaac agtgtctatg ccttgagtta
     2401 ctatctctat cagcgaagca ctatcaaatg gttaccggaa gaacatgata ttatccaact
     2461 aatcaaagaa ttagaacaac aagtttctca gtcttatcca tctagttatc actatgtcca
     2521 acgaattctg gaattaagta agatcaaatt agatattgaa gacacaagta tggatcggat
     2581 tttactgacg atctatctaa aaaaagcgaa atggtcaaaa gaaaaacgac tgccaaaagc
     2641 gatgattgct gctcacggtt atgcaactgc tagcagtatg gcaaatgtag taaatcgttt
     2701 attacaagag gatctatttg aatcatttga tatgccacta gatgtcacac cacagcagat
     2761 tgcttcagaa atccttgatt attgcgaaaa cagcgatgtt tcaaatggat tgatcatatt
     2821 agtagacatg ggatctctga aagaaatcca tcagtttttt aaaaaacagt tatccgtacc
     2881 gcttttgatt ttgaataatg tgacgacacc tttagcaatc acagtagggg aatgcctgca
     2941 aaaaaatgta tcattagaag agatagcaga agagactgtt cgacagatcc agccagaatg
     3001 gcgactgctc tatccagagg aaaacaaacc caaagcactc attactactt gcttcactgg
     3061 aatcggtacg gcggtccact taagtgaact gctagaaaaa agcttaccta cggcttgtca
     3121 gttgaaaatc atcccatatg aatatcagca attaaaagat aagaaaaaca gtgacccatt
     3181 attttccata tacgaagtcg taggaatgat tggcaccaca gatcctgata tcaccaatat
     3241 cgactatctt tcattagaag acctcatctc gggggaaaag atgtctgtac tagctgaatg
     3301 gttggacact actatgaatg cttcagagaa ggaaatattc aatcagcgca tcattcgtaa
     3361 tttttcattg gaaaaagtac tggattccgt gactattcta gatacagaga aggtgatggg
     3421 agaaatcgat cattttatga gagaattaga ggtgcagttg aatcgaagca tcagcaatcc
     3481 caaaaaatta gcgttgtatg tccatgtgag ctgtctcatt gaacgattga tccgacaaat
     3541 gccaatcgaa acctatcaag gattagagaa atggaagcag tgtcaaaaag aagggttatc
     3601 tgctattaaa tctgcgttta gtgtcattga agagcattat agtgtcatga tccccaattc
     3661 tgagattgcc tatatctatg atattttgag tgaagataca gagtttctct cgatagaaga
     3721 agaattttga acataagcaa gttgttgcca ctaattttgg catgcaactt gcttttttag
     3781 tatgtgtaag tgaaaagagg agatgaaaaa gtggatagag aaattatctt agcttcccat
     3841 ggaaaatttg cttcagggat attagattca ttagagctga tctatgggaa aaatcattct
     3901 attactgtat tcgattgcta tactgatgag aattttgatt tagcggaaac agtaaggcag
     3961 ctttttatga ggtacaaaga taaagaaatc attgttctga cggatctttt tggcggaagt
     4021 gtcaacaacg aattcttaca atatatcaat cgggatcatg tctatctcgt tgccggattg
     4081 aatttgccac tattgattga gctagtatct cggttggacg ctgaagtaga gacaactgtt
     4141 ttgatccagc aggtgctaac tgattcaaaa aaaatgatcc agttttgtaa cgagcggctc
     4201 aatcagaaaa tagaagaaga cgaattttag gaggaagaaa aatgattgta ttgacaagag
     4261 tcgaccatcg gttattacac ggacaagtag ctttttcatg gacacaaaca ataggcgcag
     4321 actgtatttt gattgctaat gatgatgtac ctacgaatga gatacgaaag acaacgatca
     4381 aattagccaa gccacaagga gtcaaactag tgatcaaatc aattgatgac tcgattgccg
     4441 cgttgaaaag cggggtgaca gataaatata agttattcat cgtagtggaa tcgatcgagg
     4501 atgcatataa gctggcagaa gcgtatccag aaatcaaaac catcaactta ggagggatca
     4561 aggcaaaacc tggtactcgc agcatcggaa aagcggtaaa tattttacca gaagaggagg
     4621 aacgcttgaa acgattaatt gacaaacaaa tagaagtaga aatccgtcaa gtaccagcag
     4681 ataaaaaagt ggatgtaaga gaagttttgt aagaaaggaa gaacctcaat gattgtacaa
     4741 gcagtcttat taggaattgt tgcatttgtc gcacaatcag aatatgcact agggacatcg
     4801 ttgctttctc gtccgattgt gacggggctg tttacaggaa tcgtgttagg tgatgtaaaa
     4861 actgggatca ttatgggcgc aactttagag ttagcattta tcggatcgtt ctcagtaggt
     4921 gcttcgattc cacctgatgt tgtgaccggt gggattttag ggacggcttt tgctattaca
     4981 gctggagccg gtacagaaac cgcgctttta ctaggtttgc cgattgctac gttgacattg
     5041 atattgaaaa atatttactt agggcttttg attccaatta tgaaccataa agcagacaat
     5101 tatgcagaag aaggaaatta taaaggagtc gagcatatgc acttgcttgc cggtatcggt
     5161 ctttccttga tgcttggtgt tgtggtaatg gtttcttata tggtgggaag caatacgatc
     5221 ggaaatctat taaatatgat tcctgacttc gttcaaaagg ggttgtcagt agctacaggg
     5281 ttgattccag ctcttggttt tgcgatgctt gctcgtttgc tgatcaacaa acaagttgca
     5341 ccatattttt tcttaggttt tgctatagct tcttacttag agattcctgt gaccggagta
     5401 gctatttttg gtgcgatttt agcagttgtg gtagtcaata ttatgaatgt ccgtcaacta
     5461 ccacttcaaa caactgcagg ggaggtagaa gaagatgaag aattctgaga tcaaaaacag
     5521 caaaataacg aaaaaagagc taaatcacgt attttggcgt tcttttcaaa tggagttttc
     5581 ttggaattat gaacgtcaga tgaatatggc ttatgtttat gcaatgattc cgattttgaa
     5641 aaagttatat cagtcgaaag aagaaatggc agcagctttg aaacggcatc tagagttttt
     5701 taacacgacc ccacatatcg ttacactgat tttaggaatc acggctgcaa tggaggaaga
     5761 aaataaggat gatcctgaat ttgatgtgac tgcgatcgat agtatcaaaa catctttgat
     5821 gggacctctt gcagggttag gagattcttt cttttgggga actttgcgat tgattgccac
     5881 aggtgtgggt acctctttag ccatgcaagg aaatatttta ggccctatct tgttcatttt
     5941 gattttcaat attccgcaca ttctgtttcg ctatctagca acaggttggg gatatcgctt
     6001 aggaacgggt tttttgaaaa agatccaggc aaacggtatg atggaaagtt tgacattagg
     6061 cgcgtctatt attggactga tggtcgttgg agcaatgact gccacgatga tcaccatcaa
     6121 tattccttta aaaatcggtt caggagaaaa tgctgtgact gttcaaagta tttttgatga
     6181 tatcgttcca aatatcttat gtcttggagc atttggcata gtgttttatc tattgaagaa
     6241 agaagtgaaa ccattaacga tcttgatcgg actagctttc tttggaattt ttggttcgtg
     6301 gatagggatt ttttaaaaaa ggagcagaga aaatgtttac gatgcaagat tatatctatg
     6361 aagaaaagga agtactaagt acgatcttga aaaagaatga tttttcgaca agagaacata
     6421 tgaaaaaaac agtgaattta ctgatcttag caacgggttc atcttataac gcttgtttag
     6481 cagcgaagcc agcattggaa tcttatggtg atcttactgt cgatattcaa gaaccttttt
     6541 attttttcaa ttatggaaaa ctttctcctt caatcgatac tgtgattgct gtgtcacaaa
     6601 gcgggaaaag tgcttctact gttgaagcag taaaaatgat acaaaaacaa gggttacctg
     6661 ttgttgctat cacaaatgac gtgcaaagtc cacttgcttt agaagctgca caaattattg
     6721 atttaggtgc cggggtagaa tcagtaggct tcgttacaaa aggctattct gcgactgttt
     6781 tacagttact actcttaggg ataggaattg gaataagtaa aaataaaatc agcaaaaaaa
     6841 tagaacaaga ctacatgcaa cagttgcgca aaataatcaa ccatttacca gcgattattc
     6901 aaaaaacgga agaatttttt gatggatatc aaagtttatt tcgtttagca cagcgtttcg
     6961 tcaccattgg atatggtcca aactggggaa cagctaaaga agcagagacg aaattgactg
     7021 aaacaatccg cgttccgtca caaggatttg agttagaagc ttatatgcac ggcccatacc
     7081 tggaggcaga tgcttcgcat ttgctattct ttattgaagg ggaaagtgta aataaggaac
     7141 gttcgcaaaa gttgcaaaga tatatgagtc ggtacgttgg agagactctc accattacca
     7201 caaaaaaagc aagaaacgag aaaacacttg gattagcaat agaatgtgat gaatatctgt
     7261 ctgtattagc gcttgtcgtt ccgtttcaac tattcgctta taaaaccgca gtagctaaag
     7321 gtattgactt gaacaaaaag atttttgaag actttgatac agtcttgaaa agtaaattat
     7381 aagaggtgga aatcatgctg aaattcaatg aagaagaaca aatcaaagca ataaaaggcg
     7441 cactagcttt acgtccgcaa gtagaagaaa tcatcgataa actttatgcg gaaaaatttg
     7501 acgctgtcta ttatctagga atcggtggaa cgtatgcttc ctcgatgcaa gctgtcactt
     7561 atatgaatgg gaaaagcaac ttgcctgtct ttgtacagca tgctgcagaa tattacacta
     7621 ctggaaacaa aagattaaca aaagattcta tcgttatttt gtcctctgtt accggtacaa
     7681 ctcaagaagt agtgaaagca gtagaacaaa tcaagaaagt aggagcgaca ctgattggat
     7741 ttatcgataa agcgaacagt aagttgtctc agctatgcga ttttgtggtc acttatccag
     7801 ctcctggaac agaacaaatc aaatttttta tggcagcaga tcgattgatg tatttgaatg
     7861 gagaattcga agcttattca gaatactatg aacagctcga acgttattta ccaacagggt
     7921 tagtggaagc agagaaaaaa gcagatgcat ttggcttatc atttgcagaa aaacatcgac
     7981 gtgatagtat gcattatttt attggagcag ggaatcaatg gggagctgtt tattcatatg
     8041 ccatgtgtta ctgggaagaa caaagctggc ttccatcgaa atctatccat gctgccgagt
     8101 ttcttcacgg gacgttagag atcgtagaag aaacaacccc agtcacatta tttttaggcg
     8161 aagatgaaca gagagtgtta tctgaacgtg tggctaaact tttgccaaaa atttgcagca
     8221 attatacatt gatagacaca aaagactatc cagtagaagg tatcagtgaa aaatatcgcg
     8281 gacgtgtttt gtctttctta ttgatgcatg tagtgacaca acgcatcgat gctcatgtgg
     8341 agcagttgaa ctgccatcca ttagagatac gccgctacta tcgtcagttt gattattagg
     8401 atagactgga taaaaagaat ataaaaaata aagagagcag cgataactgc tccctttatt
     8461 tttttaaaat attggcatca attacgttat aaaacacatt ttttgcatta tctacagttc
     8521 aacttataat ctgtttaatg ggacatgtgg gtgccaacca ttttttgtta tgcaatagta
     8581 ctatttagtt gttgcatgga tccgtagtaa aagtccaagt acaacgagtt gcgccagttt
     8641 acatagcgtt ttgtatccag aatgttgact aaaacaaaag ttcctatgtc aaccgtcaac
     8701 agagacaatc tgtccgttgc aggacctgac agatttagaa atgttcaatc tgtctcttat
     8761 gtattttaaa tcaatactca ctaggagaag tttcctaacc tcctaatagt aatgctggta
     8821 gtaacattgt caaaactgct tcctcttagg aacaagcaaa gcataacgag tgtaaaaata
     8881 aaatgtagac tattgtaaca tatcattgat tatttcaagt aaaaacgata aaagaagtaa
     8941 acaaacactt aaaaacaatg gaaattagtc ccaactttaa gaaatgtata ctataatgga
     9001 aattatcaat tatttatttg gaggaatatc catgaaacaa aaaatcgcag ttcttggtcc
     9061 tggatcttgg ggcactgctt tagcacaaac attggcagaa aacgggcatg atgtccgtat
     9121 ttggggaaat gtacctgaac agattgatga aatcaataag tatcatacaa atcagcattt
     9181 tttacctgat ttgacgatcc cagaatcgat tatcggatat aaagaattgg cagaagcagt
     9241 ggataacgca gatgctattt tatttgttgt tccaacaaaa gcgattcgtt cagtcgcaca
     9301 ggaacttatt ggtaaaatga atactaagcc aatcattata catgcaagta aaggactaga
     9361 acaagatacg cataaacgaa tctcagaagt tttggctgaa gaaattccag aagaaaaaag
     9421 acaggcaatt gttgtattat ctggtcctag ccatgctgag gaagtggcag tacatgatat
     9481 cacaacaatt acagctgcaa gtattgatca aaaagcagca gcctatgtac aaaaactgtt
     9541 catgaatgat tatttcagga tttatacaaa taatgatgtg attggcgtag aaactggtgc
     9601 agcactgaaa aatatcattg caattggtgc cggcgctatc catggattag gattcggaga
     9661 tgatgcaaaa gcagcaatca tgactcgagg attggcagaa atcagccgtc ttggtgtcgc
     9721 gatgggtgcc aatcctctga catttatcgg tttgagtggt gtgggggact tgatcgttac
     9781 ttgtacaagt gttcattctc gcaattggcg tgccggtaac ttgttaggac agggacataa
     9841 gctggaagaa gttctagaaa acatgggaat ggtcgttgag ggtgtggcaa cgacaaaagc
     9901 agcagtagag ctagctcaac agctaggtgt tgagatgcca attactcaaa caatctataa
     9961 tgttttgtat aatggagaag atatcaaaaa agcagcaaaa gaaatcatgt tacgtgacgg
    10021 aaaaatggag aatgaatttt ctcttcgtta aatagatgat caatagttaa tcgt
//
LOCUS       DARBFO010000092         9814 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000092 DARBFO010000000
VERSION     DARBFO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.094"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="SCK51_002403"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="SCK51_002403"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEG1252980.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="SCK51_002404"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="SCK51_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEG1252981.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="SCK51_002405"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="SCK51_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEG1252982.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="SCK51_002406"
     CDS             2857..3732
                     /locus_tag="SCK51_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEG1252983.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="SCK51_002407"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="SCK51_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEG1252984.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="SCK51_002408"
     CDS             4629..5063
                     /locus_tag="SCK51_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEG1252985.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="SCK51_002409"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="SCK51_002409"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEG1252986.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="SCK51_002410"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="SCK51_002410"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEG1252987.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
BASE COUNT         3435 a         1693 c         2197 g         2489 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaat
//
LOCUS       DARBFO010000093         9713 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000093 DARBFO010000000
VERSION     DARBFO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.095"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            33..656
                     /locus_tag="SCK51_002411"
     CDS             33..656
                     /locus_tag="SCK51_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252988.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /locus_tag="SCK51_002412"
     CDS             1106..2155
                     /locus_tag="SCK51_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1252989.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="SCK51_002413"
     CDS             2152..3033
                     /locus_tag="SCK51_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1252990.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="SCK51_002414"
     CDS             3036..3806
                     /locus_tag="SCK51_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1252991.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="SCK51_002415"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="SCK51_002415"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEG1252992.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="SCK51_002416"
     CDS             5117..6280
                     /locus_tag="SCK51_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG1252993.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="SCK51_002417"
     CDS             6397..6807
                     /locus_tag="SCK51_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1252994.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="SCK51_002418"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="SCK51_002418"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEG1252995.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="SCK51_002419"
     CDS             9380..9664
                     /locus_tag="SCK51_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252996.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT         3371 a         1646 c         2087 g         2609 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgt
//
LOCUS       DARBFO010000094         9625 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000094 DARBFO010000000
VERSION     DARBFO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.096"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            99..293
                     /locus_tag="SCK51_002420"
     CDS             99..293
                     /locus_tag="SCK51_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252997.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..861
                     /locus_tag="SCK51_002421"
     CDS             562..861
                     /locus_tag="SCK51_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1252998.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(958..1392)
                     /locus_tag="SCK51_002422"
     CDS             complement(958..1392)
                     /locus_tag="SCK51_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1252999.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1624..2334)
                     /locus_tag="SCK51_002423"
     CDS             complement(1624..2334)
                     /locus_tag="SCK51_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEG1253000.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2324..3184)
                     /locus_tag="SCK51_002424"
     CDS             complement(2324..3184)
                     /locus_tag="SCK51_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEG1253001.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3189..3827)
                     /locus_tag="SCK51_002425"
     CDS             complement(3189..3827)
                     /locus_tag="SCK51_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEG1253002.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3842..4141)
                     /locus_tag="SCK51_002426"
     CDS             complement(3842..4141)
                     /locus_tag="SCK51_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1253003.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4184..5668)
                     /locus_tag="SCK51_002427"
     CDS             complement(4184..5668)
                     /locus_tag="SCK51_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEG1253004.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5688..6149)
                     /locus_tag="SCK51_002428"
     CDS             complement(5688..6149)
                     /locus_tag="SCK51_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1253005.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="SCK51_002429"
     CDS             complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="SCK51_002429"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEG1253006.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7452..8222
                     /locus_tag="SCK51_002430"
     CDS             7452..8222
                     /locus_tag="SCK51_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1253007.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8495..9322)
                     /locus_tag="SCK51_002431"
     CDS             complement(8495..9322)
                     /locus_tag="SCK51_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1253008.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         3002 a         1960 c         1504 g         3159 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat
      901 cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta
      961 tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc
     1021 catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc
     1081 atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt
     1141 caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat
     1201 aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc
     1261 attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt
     1321 tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga
     1381 atcaatattc atttttttct tcctttctta tacaattctc cctctaataa tacagttctt
     1441 tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt
     1501 cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt
     1561 cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca
     1621 tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa
     1681 atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca
     1741 aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa
     1801 cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt
     1861 aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt
     1921 cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata
     1981 agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt
     2041 aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga
     2101 tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc
     2161 tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg
     2221 gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag
     2281 atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc
     2341 cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt
     2401 cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca
     2461 aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact
     2521 ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat
     2581 cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta
     2641 ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg
     2701 aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag
     2761 aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt
     2821 tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg
     2881 ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag
     2941 aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc
     3001 tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc
     3061 gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg
     3121 aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag
     3181 ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc
     3241 ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca
     3301 aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca
     3361 atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca
     3421 tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt
     3481 tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc
     3541 tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca
     3601 acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta
     3661 tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt
     3721 gtgcccactt caataatatc tactacatca ccaactttca taacatctcc taatgcatgt
     3781 tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc
     3841 tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg
     3901 acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat
     3961 tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg
     4021 aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca
     4081 cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca
     4141 tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt
     4201 ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt
     4261 gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca taacctagga
     4321 ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac
     4381 caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga
     4441 ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa
     4501 ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg
     4561 atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc
     4621 caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa
     4681 cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga
     4741 atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca
     4801 taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt
     4861 tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca
     4921 tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt
     4981 gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa
     5041 atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt
     5101 cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc
     5161 ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa
     5221 aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg
     5281 tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca
     5341 tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc
     5401 caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga
     5461 ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg
     5521 ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta
     5581 ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg
     5641 tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct
     5701 gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca
     5761 tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt
     5821 gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa
     5881 cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt
     5941 tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct
     6001 acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca
     6061 agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc
     6121 aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg
     6181 gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact
     6241 caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt
     6301 taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat
     6361 cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg
     6421 actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt
     6481 caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat
     6541 tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt
     6601 ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt
     6661 cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga
     6721 ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt
     6781 gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa
     6841 ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt
     6901 ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca
     6961 tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat
     7021 gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt
     7081 tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt
     7141 ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact
     7201 tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt
     7261 ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg
     7321 tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag
     7381 atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg
     7441 aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg
     7501 aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat
     7561 ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca
     7621 attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg
     7681 aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc
     7741 tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt
     7801 tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt
     7861 atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat
     7921 taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg
     7981 tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca
     8041 acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa
     8101 agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg
     8161 aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt
     8221 aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg
     8281 ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa
     8341 tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat
     8401 caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg
     8461 gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct
     8521 ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta
     8581 aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca
     8641 atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg
     8701 ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga
     8761 ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt
     8821 tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta
     8881 agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg
     8941 catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta
     9001 aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt
     9061 ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt
     9121 cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta
     9181 agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac
     9241 aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta
     9301 atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta
     9361 tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt
     9421 aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt
     9481 gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc atggcgagaa
     9541 agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa tgacgacagc
     9601 acacctatca attaatataa ttatt
//
LOCUS       DARBFO010000095         9419 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000095 DARBFO010000000
VERSION     DARBFO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.097"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(93..761)
                     /locus_tag="SCK51_002432"
     CDS             complement(93..761)
                     /locus_tag="SCK51_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1253009.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            895..1182
                     /locus_tag="SCK51_002433"
     CDS             895..1182
                     /locus_tag="SCK51_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253010.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1598..3136
                     /locus_tag="SCK51_002434"
     CDS             1598..3136
                     /locus_tag="SCK51_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEG1253011.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3133..4098
                     /locus_tag="SCK51_002435"
     CDS             3133..4098
                     /locus_tag="SCK51_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1253012.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4085..5407
                     /locus_tag="SCK51_002436"
     CDS             4085..5407
                     /locus_tag="SCK51_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1253013.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5470..5574
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5648..6391
                     /locus_tag="SCK51_002437"
     CDS             5648..6391
                     /locus_tag="SCK51_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1253014.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6406..7398
                     /locus_tag="SCK51_002438"
     CDS             6406..7398
                     /locus_tag="SCK51_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1253015.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7410..8147
                     /locus_tag="SCK51_002439"
     CDS             7410..8147
                     /locus_tag="SCK51_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1253016.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8137..8859
                     /locus_tag="SCK51_002440"
     CDS             8137..8859
                     /locus_tag="SCK51_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEG1253017.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8943..>9419)
                     /locus_tag="SCK51_002441"
     CDS             complement(8943..>9419)
                     /locus_tag="SCK51_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEG1253018.1"
                     /translation="NSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQ
                     EAPVQAEQPVVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGS
                     YTATNGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3089 a         1613 c         1984 g         2733 t
ORIGIN      
        1 atttttttat aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc
       61 tgtaaattaa ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca
      121 agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt
      181 tccagatact tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac
      241 agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag
      301 aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct
      361 gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata
      421 cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc
      481 cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt
      541 tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca
      601 gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct
      661 gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa
      721 atgattccgc acaattctgt aaataaattt aattttttca tttctagcct cttttctttt
      781 gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg
      841 aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga
      901 gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt
      961 tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat
     1021 caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt
     1081 tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct
     1141 ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa
     1201 gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac
     1261 agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta
     1321 ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc
     1381 aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag
     1441 ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc
     1501 ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa
     1561 atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt
     1621 aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat
     1681 ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa
     1741 aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc
     1801 cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact
     1861 tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga
     1921 cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa
     1981 agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt
     2041 aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat
     2101 tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc
     2161 cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga aacctcgata
     2221 ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc
     2281 tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat
     2341 cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa
     2401 accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat
     2461 ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa
     2521 aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca
     2581 attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa
     2641 gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga
     2701 tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat
     2761 ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta
     2821 ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct
     2881 caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg
     2941 cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa
     3001 taaattaagc ggaaaaataa cggaggatca aatgattgat atgaattacc aagtcaatgt
     3061 cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa
     3121 ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga
     3181 aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt
     3241 ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca
     3301 tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg
     3361 cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag
     3421 caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt
     3481 gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct
     3541 agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc
     3601 ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca
     3661 ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg
     3721 cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa
     3781 attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg
     3841 catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat
     3901 ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct
     3961 atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa
     4021 agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa
     4081 ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc
     4141 ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg
     4201 gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg
     4261 taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga
     4321 agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca
     4381 attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc
     4441 tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag
     4501 acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc
     4561 attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct
     4621 agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc
     4681 attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg
     4741 cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg
     4801 tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca
     4861 gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat
     4921 tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt
     4981 ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa
     5041 gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta
     5101 cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca
     5161 agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc
     5221 taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga
     5281 gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa
     5341 agagttggat agctatatca tggggtatcc gacattggca agtgatctct cctatttatt
     5401 gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta
     5461 aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta
     5521 gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc
     5581 gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg
     5641 taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg
     5701 gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt
     5761 attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa
     5821 tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat
     5881 ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt
     5941 gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa
     6001 agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt
     6061 gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa
     6121 gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt
     6181 ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg
     6241 cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc
     6301 gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa
     6361 agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg
     6421 cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc
     6481 aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact
     6541 aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat
     6601 gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa
     6661 gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat
     6721 ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta
     6781 tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa
     6841 agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca
     6901 aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat
     6961 atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta
     7021 aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt
     7081 acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg
     7141 aaaaaaggct atcaggaagc tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa
     7201 tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat
     7261 tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca
     7321 gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact
     7381 aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag
     7441 tcattgaaaa cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta
     7501 catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag
     7561 tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata
     7621 cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa
     7681 acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt
     7741 atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg
     7801 ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc
     7861 ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt
     7921 tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag
     7981 caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg
     8041 agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga
     8101 agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat
     8161 ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc
     8221 aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa
     8281 gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag
     8341 ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat
     8401 gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat
     8461 gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa
     8521 gcttacccta atcactattt gatccattgg tattcttctt tggactatca acaagactat
     8581 attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg
     8641 caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca
     8701 gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa
     8761 cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact
     8821 tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta
     8881 gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt aataaatgaa
     8941 tcttagtacc aaccgtttgc ttcccagaat gctttcgccg cttcccaaga accataacgt
     9001 gaagtaacat attgttctgc tactctttct tggtttgcag cagagtagtc accatttaag
     9061 taagaagaat ctaattggta acgtccgatg taacgtccgt tcgttgctgt gtaagaaccg
     9121 cttgattctt tttgtgcgat ccattctttt gcagagcttg tgcttgctgg agtagcagca
     9181 gtttctgtta cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg tttttccgtt
     9241 tgaaccgttt cttgtacaac aggttgttca gcttggactg gtgcttcttg aacggctgct
     9301 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
     9361 taaatcaggt taatatcaga aattgaattt gattctgcaa tggcattgat caaggagtt
//
LOCUS       DARBFO010000096         9374 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000096 DARBFO010000000
VERSION     DARBFO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.098"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..410)
                     /locus_tag="SCK51_002442"
     CDS             complement(<1..410)
                     /locus_tag="SCK51_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1253019.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFL"
     gene            complement(400..2574)
                     /locus_tag="SCK51_002443"
     CDS             complement(400..2574)
                     /locus_tag="SCK51_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HEG1253020.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYFSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2590..4932)
                     /locus_tag="SCK51_002444"
     CDS             complement(2590..4932)
                     /locus_tag="SCK51_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253021.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4954..7497)
                     /locus_tag="SCK51_002445"
     CDS             complement(4954..7497)
                     /locus_tag="SCK51_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1253022.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7507..7905)
                     /locus_tag="SCK51_002446"
     CDS             complement(7507..7905)
                     /locus_tag="SCK51_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1253023.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7986..8270)
                     /locus_tag="SCK51_002447"
     CDS             complement(7986..8270)
                     /locus_tag="SCK51_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253024.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8294..9292)
                     /locus_tag="SCK51_002448"
     CDS             complement(8294..9292)
                     /locus_tag="SCK51_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1253025.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2669 a         2004 c         1466 g         3235 t
ORIGIN      
        1 agaaattgat tcaatgtctt ttcaaaaaag aatgtttttc tctcaatttt tttgacgttt
       61 ctatcatgaa tcacgagaaa acttgggaca cttttaacat ggtaacgttg taaaaaagtt
      121 tgaagttctt cttgtcttgg ttcttttctt gtttcaattc gatatacatt cctagagaat
      181 tttttgtcgt atctttccaa gaaagattca acttcttggc attttttaca atcatttctt
      241 gctagataca caaaaaatga ttgattcgtt ttttgaagtt ctagtacttc ttctaaatta
      301 ctattgatta gttttccatt tgattcttcg ttagttttta tcagtttaaa acaaccaaat
      361 aagaggaaac taacaataag tatcctaaca ataactttct tatttcgcat attgtttctc
      421 caaatttggt ttccgaatat agacgactcg cggtggttcc tggacgtctt cagctgttcg
      481 atacaccaca aattcgcctg ttctcaatgc tcgaatgcga ttcggattga atttaaagcg
      541 gtctacgtct cgtttcgtgc ctctttctcc tgtccaatca acctttccca tatcagaata
      601 acctgcttct tgtgtcgtca tttccgttaa ttcaatgtct tcttctgtcc ccattaaatc
      661 tgcccaataa tcaatttctg tcgggtcatt tgtcttccca ataaagaacg tattcacatt
      721 cccaatcaac tgttttcgaa ggacaggatc aattctatca atgtccgcaa gcgtttgagg
      781 ggcatacgcc gcatgcaccc cagcgcctcg tcccttattg actaagtcta taaggttatc
      841 atttagataa gaagctggtt cgtcaaatat tactaggact ttcgagcgat tggctgtttc
      901 tcctgcttgt ttatacactt ctgacgcaaa gaaattaata tctcccacaa gcatttgagc
      961 caatgtccga ataaactcac tataaatcaa tccgtttaac gagatataaa caatctttcc
     1021 ttctttaaca acttcttgaa tatctaattc tctttttcct ccagaagtat caaagagttc
     1081 accaagctca ctatccaata acatattgat attggtctgt aaattctcag aagtcgcata
     1141 caaataaaac gggttcttct tatgttcgta acgaacaaac aaccgtttaa acaacgtttg
     1201 ctcgatttct gtcatgagag aaatgttttc ttttaataat agataatacg tttctaagtc
     1261 aacggtttta cgatcatgtt caacgattcg ctcttcactt aactgttcca catcggcaaa
     1321 atcacgttct gttttgtcca tcgcttcatt ctttttcgtt tttttcttcg tggttttctt
     1381 cttttctacg accacttttt ctttaaataa atctggattt ttcaactgaa tacgatccgc
     1441 aaacaaatgt aagacgtttc gtggcaataa ataatgttgt aaccaaacta gatttctttc
     1501 aattttatgg ctcttttgga actcgtctaa aagctgaatc gttacctgta ataacgcttt
     1561 ggctgctgtc gaataataca cgctttctgt ttctgctaga ttggttaatt tatctcgaat
     1621 ggcaatatca ttgccatttt ttacaggatt ataacgcaaa tcgccaccgt ccacaaagac
     1681 ttcaatcggg cgattgtatg cctctgccac ttcttttaac gcttgtaaag tctctcctgc
     1741 tcctttccca tcgactaaga taacggggat acgaaactta cacgcatgtt ctgctagtaa
     1801 ttggatcacc gtcgtttttc ctgaacctgt agtcgctgga ataatcacat gttggttcaa
     1861 ttcactaaag ttcatcacat aagggagccc atactcatct gttcctaata gaatcacttt
     1921 ggctaatgaa gccagttgtt tattccgcaa agcaacgtct tttatgacaa ttgctacctg
     1981 gtacgcttgc tgatactctg ctgtatattt ttgggactcc tgaaatttat tcttccgacg
     2041 ttccttgtat tctttactat ttagcaaagc tgtttgccgt tctttctttg aaacaacttt
     2101 tcttttttca ataaaagacg catacgcaat caaacaactt cctgcacctg tcccacctaa
     2161 aaggagggca aaaatcgttt gtccattaaa ctgcaacgca tgagaaaaca agtttaatgg
     2221 aaataataag taacacgaaa aataccctct aggaagaaca aaaataaaac aaaatagatt
     2281 taatagcgta aaacctaacg caaaaataaa aaaaagagag cttcttcgtt ttaagaagct
     2341 ctcatactca aagtcataaa tatgtgcacg tgtctctttt ttatctatca taaagacaat
     2401 cgctaaagga atactgccta caataaaaaa tggatataga caaacaccta ataaagtgac
     2461 gataaaataa agttgttgaa accgactttt tggcgtttct ttttcttgtt ttcttttgcg
     2521 atcgacagaa aacatttgct caaaatgtgt cggtcgtttt ggtgcattca tcatactatc
     2581 gatttcctcc tatcgttttc gttgtttcat ttctcttcga taaatcggtt gtttttctcg
     2641 ttttcgtttc atttttgctt gagttgctcg aaactcactc cgtgtcttcg gtgcttgttt
     2701 tggtgatttt gtttgtttca tggttgagta tgctttagat acggttgaag gcgctttggt
     2761 ttgtgctgtt actcgattga ctgctgacat atgttcccga accccctctg gacgtcgaac
     2821 agatttcggt gtggtagaac ctgttgctcg tggtacattc ccttttctta gtggtttttg
     2881 taccgcatgt ttttgtcctg caactgtccg ggttttcatc gaacttgttt ttgttacggg
     2941 agtaagtgcc tccttccgac gacgacttgt ttgtccctga cgacgttttc gatcgttcaa
     3001 ttctgttcta ggcgtatgct tctggccata agacttgtgt ttttgctttg gattcgctaa
     3061 tgacaccacc attgcagaac cctgattgcg atacggacta cgagacgcac cttttctact
     3121 tctttctctt agactaggat tgccttgcaa ttctcgttgt aataaatcgg ctgtttttct
     3181 tgcctgtttc tccattgctg ttcgttgcat acgttcttga ctttgctctt ctgcttgttt
     3241 cgcttgatca tgcaagtctc ttgctttttg tgccatacgt tccatacgat ttggtgcttt
     3301 atgacgaggt tgttgtgcgg attcacgccg aaaagcactt cgtgtggctt gtttcgccgt
     3361 ttgtggcata cgaacgtctg agccaccccc acgttttcgt tgacgttctt gtctcttccg
     3421 tgcgttttct ttttcttttt gtttctgtgc ttttctcgct tctttgcgtt ctttcaaccg
     3481 ttgtttggct tcccttctca attttgcttc tgccccaaat ggatttcccg caaaagctaa
     3541 cgctcctcga acacccattc ttccagtaaa tagactgcct aaaaagcctc ggaacaaata
     3601 aattcccaac ggcgcaaaca aataaacaat gatcaagaga attttttccc atggatcgtc
     3661 ttgtgtacta cttaattgga acccgtaact aagaaaagaa gaaaaaagga tacatgcaaa
     3721 acctgtcatt ccttttaaaa acgtcaaaat accaaaaact ttggcatagt taccaaacac
     3781 attcattgtg acaagaaaca atcctaaaaa aagtaagaac ggcaacatcg gtactaacaa
     3841 tagttgcata gttccaataa tcaatcgaaa catacttaag acaaaataaa caacggtttg
     3901 aatcagattc acgacaaaat aaaacaaggc aaatgctgta tttttccaac tattgtaata
     3961 ttgtatgttc ttgtttttca gtgtatctgc ttcatactct gtgactttgt catgaatttt
     4021 ctcccctatt tttgtaccta aatcattgtc caataaaaga ttgattcgat catattcttt
     4081 tccagcatat ttgactttct tctcacgaat tgtgtctaca ttggttgtgc cataatttaa
     4141 caacagatat ggttcataaa cattactata aaagacccga gccgccatca atttgcctgc
     4201 cgtttccact tttttatccg ctgttacctc ttctgtttct tttgtttcat aatttgtttc
     4261 ttcttcaggt gttaacactg gattgacctt cacgaacgcc gtttccactg tagaatctaa
     4321 agaaaaaaga acatcaaaaa gattcttatt tgtcccagaa tcttttaata cgcttaaccc
     4381 agtaaaaata aggatcgtca taagaaagac acggagtgct tgtttcattc gagcctctcc
     4441 agcatatttg attgccatca taattgccga aaaactgatt ccaatagttc ctgcaatact
     4501 taacatattg tccgcaattt tttgtgttaa gttttgaatg ggttctcgaa tggcggaaat
     4561 aatatctaat tcaaagagaa attcaaccat tgaagcattg aattctccta acatttttac
     4621 gccactccaa acaaaatttt tcatcactcc tgcaacattg tacgatagct tttttttaag
     4681 gttcattgac tcatagtcat tggtcatcaa ttcaaacgaa ttctttttgt aatttttata
     4741 caattgatcc acatcagtcg ttacttcttt tacttctact ttatcgttgg cattctctcc
     4801 ttcagaacga tcggtttcag tgacacccgc accatcattc atttgatcgg cagaaacagt
     4861 agccccttgt agacagaaaa aaagaccccc taataaaagg agcgtccacc atttcagaga
     4921 tttttttcgc aaaaaaacca cctctttttt gttttatcgt tgtttttcat tttctaacat
     4981 tgcttgaatt ttttcatctt gattttcagt agaagaaaca gccaataacc aactatcaaa
     5041 caacacatca atggcaatcg gttgatttct tcccaaatga tcctggaata aacaggttcc
     5101 tgtttgtaat tgttccatca tttctaaatt ggctgaattc acttccatat caaagaagga
     5161 caatagtttc tctcgtgctt ctttttgttt cggacgaaac gcaaatttgt aagaaattaa
     5221 ctctttttta tcttcttgat cgtaatcttt aaacgcttgc acaacaaggt aaatatccgt
     5281 attattcgca cgtccttttc gcaagctatc atcaatcaaa tagcttcctt gtgcggtatc
     5341 ttcataccct tttgcctcgt cataaataat cgcagcgtct tcttctttgt ttgtagaaaa
     5401 gatatacgta aacttcataa tgacttccat aattgccatt cctgcaagtt gttcgctatt
     5461 taatcgaccc gaatcaatct ctttttgtgt cggaatgcgt attccttgtg tccctaacac
     5521 gttgatttgt gaatcaaaac gtatcggcgt actgtgctca ttcccaatga gaatctttcc
     5581 taaaccaacg ctatgcccta aaatcaattt agctagttct ttgtcacttt ttcgaatttc
     5641 ttcgataact tgacttaacg aatgcttacc tggcatggtg taaactacat cgatggcgtc
     5701 ttggatcacc gttttcttgt ggctcgctgt ttctcgattg gtatctactt ctcctaacat
     5761 tcctaacaca tttttagcgg tttgaattgc ctcttctttt ggtaaaaaca atagaggatc
     5821 aagcatccct cgacagcttt catctgaaga taacgaaata aaattaattc gttcatacat
     5881 agccacaaac tctggtatgt gtccgaattt ttcaagtgcc tttaaaaaga acttttgggt
     5941 ttcatttttc ggatcaatat aaagaatttt ttgacctaaa aacatcagcc ataaaaaaat
     6001 atattttaca aggaccgatt ttcctttacc tggtggacca ataatcaacg tatttccatt
     6061 ggtatgttcc gcgccttcaa tacttttctt tgttaaattg ggataatacc atactagttt
     6121 actagatagc gctaacgctt ctttcgcaga tttaaacttt tttgcaataa caatccgtcc
     6181 taaaggaaat cctttcttat ttcctacttc tttctttaag tccaagccat aatctgccac
     6241 aaaacctgtc gtagcgacct gctcgtaact acgaaaacgt tgcgtacttc caattaagca
     6301 ttgattaaac aacgttagct gatccgctac cgatcgataa aatttgaaat ctgtagactc
     6361 aatcgcaaat tcaatatctt tgactcgttc ttccaattct tcttttgaag aagcagatac
     6421 aaccagcgtg acggtagaat aacacaatcg tttttctttg cgcttcagct tatcttttac
     6481 atccattaac cgttctgtac caaaatttaa tacatcgtct gaatctaaaa tgccgtcaac
     6541 ggtctcatat tctttttctt gttcataaat ccgtccagcc attttattaa tttttcgttc
     6601 atcagatttt gaatgctcaa atcgaagtcg aatatgcgta tcaattggaa aagataagct
     6661 atcctgaaga ttttgaataa agctactccc atacatggct aaaggagcgt ccgtttgact
     6721 taaaaacgtc gaataatgcg tttgattcaa ctgttcaatc gcaatgtatc ctgcttcatt
     6781 cgaaatgacg ccctcgcaaa tttccattgg cgtaagctgt ctttcaggca atttttcttc
     6841 tgatcgatgg aaaaaataat aataaatttg tccaatctct tgggcagata agcgctctat
     6901 cggcttataa ttggacaatt ccgtatagag ctccttttct aatgtttcat acgttggtaa
     6961 cacactactt ataggaactc gttgtcccgt aatacgatag ataaactttt taaaaacatg
     7021 cttaagcaat tgaaaatatt cgatcggatc agacactttc atttctgtag tgaaacgcac
     7081 aaacagatac gttcgatact cgtttaattg cacttcgtct tttaaaatct ttcctgcctg
     7141 ttcaaagtac actttcccaa gatctgcgca ttctcccttt accaaatctc gaatggtttc
     7201 ttcaatgtgc gtatctaggt taaattgacg tggaatattg ataaaatgat agtcatattc
     7261 atcatgtcca agtatgcctt cgccatcttg aagatagttc ttgaaaaaat caatgtcatt
     7321 taaaggaaat acttgggaag aaagttgata gcctacccac acatcatcat ttttggtcaa
     7381 aacaaaattt cggataatgg cgtctatcgg aaattctaat cgaggaagct tcgttttttt
     7441 ctttttgaac tgccaaatcc cgataataaa gacgaccaaa agaaacaaaa acgccattta
     7501 ctcgactcac tccttccatt tgtgtaggga atacgtcact ttttcatgga aaaacggcac
     7561 atacatttca tgttcaaagc gttgtttccg tttctgataa taggacgttc gcccaattaa
     7621 atactcgaaa aagtgtttat tgtcccattt cattgaaaac aatgtccaaa cgagaacacc
     7681 aatcccaaaa atcataatca accaggactt tcgaaacagt gtctgcaaga aactaatttg
     7741 attcacatac gcaatcaaaa ataggatcaa aagaatgaag agaaataccc cacctatgac
     7801 taattgttcc atgcgaatgc cattagaaat ccagggaatt ttttgtcctt taatcccgta
     7861 aattttgtga gggtgcttaa actcctttgt atagttgtat tccatgttca ctctacctcc
     7921 ttttacaaaa aaagaagcga ccagaagttc taatcttctg atcgcttttg cagtttgtct
     7981 ttttcttatg ataattgtat ccagggcaca atcatttgcg ttacccccac gttctcttca
     8041 ctatccttaa taaagataat gttctttagc cattcaccaa tatcaacggc attcgataac
     8101 aataagacga gcattatcaa aataaagacg ccaataatgg caaacgctgc ccgcgcaaag
     8161 ccttgggtcg cataagcacc aaccattaga acaatcgcaa cgattgccaa tagccaaatg
     8221 acttgtccag atacatcatc aaataacgat ttaagatcaa aaagtttcat ttccattctc
     8281 ctctaagttc attttattca tttaacgtag taacaaaata actatcgcca cttgttttta
     8341 cttccattga aaagtttgat tggaactcgt ttcctttctc aaaatagaaa cgatacttcc
     8401 cttgcaaaaa gtagtttcct tctttatttt ttactgcatg atccagttgt acattgatca
     8461 attcgccgcc ttctaaccct ttttcgttag aaatcaatgc caatttttca tcatttttga
     8521 catataactc aaaaaaccgt ttcacaaatg cttccagctt gatttcttcg ctttcagtga
     8581 ccgcttgccc agaagaataa aatcgatttt cctgatagtt cacagaattg gtattcttac
     8641 tctcagaaag attcaaaaat tgaacgccat caagtacttt taaaacattg ttttgataac
     8701 tgactggaaa agaaagttga ctgcttttcc aatgttctcc ttctgtaaaa gtaacctcat
     8761 aaaataggcg atacattgct tgtgtcaaat cgatttcttc tagtttaata aagcgaacat
     8821 ctttcgctac tcgattggta gccgtcgtta caaagcctag atcagacgct tccaatcctt
     8881 gtgctaaata atgattgacg gaagcctccc attcgtttcg tttttctggc gaataagtaa
     8941 ataacctttc aataaaatgt tgtccctcgt acttcaattg ttccgtagaa gacaaggttt
     9001 ggttgacttc agttaacaat gtttctggat tgacttgttt tttcactgct aaacgcgtta
     9061 atgtatccac tcgtccaaag tttaggagga gcacgacacc cattaacaga aacaaacaaa
     9121 aaaaagcgcc tgaaaataca ctcatggcac ttttttgcga aaattggatc attcgatctg
     9181 acggttctgt tttctttttt tggaacaaaa aacgtttctt ttgtttcttt tccttctgtt
     9241 gtttcggtgg cttagtttgg aacgcttgtg gattaaattt tgcattactc atgagaaatg
     9301 ccataccgtt ctaacaataa tttcggtatt ttgtccacaa tcactacttc gtggacaacc
     9361 acacttgccc cctc
//
LOCUS       DARBFO010000097         9357 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000097 DARBFO010000000
VERSION     DARBFO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.099"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="SCK51_002449"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="SCK51_002449"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEG1253026.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="SCK51_002450"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="SCK51_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEG1253027.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="SCK51_002451"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="SCK51_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEG1253028.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="SCK51_002452"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="SCK51_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEG1253029.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="SCK51_002453"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="SCK51_002453"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEG1253030.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="SCK51_002454"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="SCK51_002454"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEG1253031.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="SCK51_002455"
     CDS             complement(8053..8523)
                     /locus_tag="SCK51_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEG1253032.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="SCK51_002456"
     CDS             complement(8569..8994)
                     /locus_tag="SCK51_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEG1253033.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="SCK51_002457"
     CDS             complement(9061..9330)
                     /locus_tag="SCK51_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEG1253034.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
BASE COUNT         2355 a         2095 c         1621 g         3286 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttt
//
LOCUS       DARBFO010000098         9072 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000098 DARBFO010000000
VERSION     DARBFO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.100"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..83)
                     /locus_tag="SCK51_002458"
     CDS             complement(<1..83)
                     /locus_tag="SCK51_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1253035.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKE"
     gene            284..1498
                     /locus_tag="SCK51_002459"
                     /pseudo
     CDS             284..1498
                     /locus_tag="SCK51_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1677..2006)
                     /locus_tag="SCK51_002460"
     CDS             complement(1677..2006)
                     /locus_tag="SCK51_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEG1253036.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1996..2268)
                     /locus_tag="SCK51_002461"
     CDS             complement(1996..2268)
                     /locus_tag="SCK51_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEG1253037.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2719..3522)
                     /locus_tag="SCK51_002462"
     CDS             complement(2719..3522)
                     /locus_tag="SCK51_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG1253038.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3500..4624)
                     /locus_tag="SCK51_002463"
     CDS             complement(3500..4624)
                     /locus_tag="SCK51_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1253039.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4640..5455)
                     /locus_tag="SCK51_002464"
     CDS             complement(4640..5455)
                     /locus_tag="SCK51_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1253040.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5445..6317)
                     /locus_tag="SCK51_002465"
     CDS             complement(5445..6317)
                     /locus_tag="SCK51_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1253041.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6402..7691)
                     /locus_tag="SCK51_002466"
     CDS             complement(6402..7691)
                     /locus_tag="SCK51_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1253042.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7919..8563)
                     /locus_tag="SCK51_002467"
                     /pseudo
     CDS             complement(7919..8563)
                     /locus_tag="SCK51_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
     gene            complement(8863..9021)
                     /locus_tag="SCK51_002468"
     CDS             complement(8863..9021)
                     /locus_tag="SCK51_002468"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253043.1"
                     /translation="MTVHWEGAHRPIARETQGGICEKMTEEILYQRLGSTSRNLIVSL
                     GHSMGISH"
BASE COUNT         2815 a         1918 c         1445 g         2894 t
ORIGIN      
        1 tattcttttt caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt
       61 aattttctag tatgattatc cattgagaga ctcctttggt aggatgacgt tttggtcgac
      121 taaattttac caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa
      181 aatcgccttc gattttccac caacacaaaa tattatagaa gcaaaatcaa ttctacctta
      241 gatgttctta tatcactcta tcagtcctat ttgacaaaga gccaagaaaa agacgttggt
      301 cgtcgatgct gttctctcgc ctactcctcg tgcttgtaga agttgtggtt ctactgtggt
      361 agatggaaac gggaaagcaa ttatagtgaa aaatgggaag aaggaaacga ttgtccgttt
      421 tgaacaatac aatcatatgc ctttggttac gcgcctaaaa aagcaacgct atacctgtaa
      481 aaactgtcgt acccattgga cggctcaaag ttattttgtc cgactcagcc attcaatcgc
      541 agatcatgtt cgatataaaa ttgcttcttt actgactgaa aaagtatctt tgtcttttat
      601 tgcgaaaagt tgtcaggtat ctttgactac cgttactcgt acattgaaag agtttaaaag
      661 ctatttacca aagccttcta agaagattct ccccagagta ttgatggttg atgaatttcg
      721 ttcgcacgct tccatagaag ataaaatgag ctttatttgc gcagatggtg aaacaggaaa
      781 cttaattgct gtcttgccta cgcgtaaatt acctcgatta acaagctatt tcttaggctg
      841 caccaatcca gaagaagtag aattcttagt cacagacatg aacgccgctt acttccagct
      901 taccaaacgt gttctgccaa atgcgaaagt agtgattgat cggtttcata ttgtcaaaca
      961 catgaatcag gcactcaatg agttgcgtat ccgtgaaatg aatgagcttc gtaaagcagg
     1021 acagaaaagt caggcagaaa aactgaaaaa gaactggcgc tttctgctaa aaaatcgtgc
     1081 aagcatcaac cattatgagt acaaaacatg gaaaagtttc cgagcaccaa aatacccatt
     1141 tctcactgaa gcaatgatga ttgatcgatt gcttgagttt tctgcgcctc taaaggaggc
     1201 gtatcccttt tttcatgaat tagtcgaagc ctttcgagac aaagaccctg acttattttt
     1261 ctccttattg gcagaacttc ccgaaacgtt agatgacggc tttcgggaga agcttcaaaa
     1321 ccttctgacc tatgaagaag gtatcaccag cgcaatgatc tatccttatt ccaatggaaa
     1381 aattgaagcg aagaataccc acataaagac aatgaaacga gtatcctacg gatttaaatc
     1441 attcgagaac atgagaatta gaatcttttt gatcaatcaa ttaatcaatg taagataaca
     1501 aaaaatctga accagaatgt ggttcattct gacccggatt tgattttgca caactcatca
     1561 gtccttattg acaaagagcc aaaaatctga gccagaatga ggttcattct gacccagatt
     1621 tgattttgta caacccatca gtccttattg acaaagagcc cttcttttta gtattattat
     1681 aattcttcca tgcgttcttc cagtcgctgt aaatcgcttg gacttaattc cccaattttt
     1741 ttccatttga cctttttgtt gtttttgggt aattcgttta gagttcctac gtcaatccaa
     1801 gattcttttt ttagtcctgc ctgtttccaa tcccttattt caacgtattt cagctttatt
     1861 ttatcggatt tagtatgata ttttgaagta attgacagaa acgaaattgt gtccagtcca
     1921 ttttttagaa tatagacagg ccggcgttta ccgccctctg cagctgtgaa agcaacgtag
     1981 acggttgcta tatcattagt attcatcttc attgaaccat tcttcaattt tttcgtcagt
     2041 gtctagtttt tggacaggca atttttccgc taatctttga atattatgga ttcgtttact
     2101 tttttccgac aattcaatag aaaatgggat ttttccttgc tcaacaattt gcttataaaa
     2161 aacggtgacg gctgttttgg ggctcatgcc tagttcatca ataatgcttt ctgcttcatt
     2221 tttcaatttt tcgtccatag aaatttgtaa cttagccatt ttctccacct ctttctgaca
     2281 aacacattat atcacggaaa gagttcaaat ggaattaata caactacttt tgaactcctt
     2341 gaaatgcagt gtatttatct aaatataagg aaaggatata gtgctaaatg gccttgatag
     2401 atagaaaagt ttcaaaatcc gccacagcga taattgaagc gaacactcaa cgacacacag
     2461 aacaggaaaa gactcctaaa agaaccaaat taactaggac gtgtctgaaa actccacaaa
     2521 gatgtggtat gctctggtaa aaaacgaatg gcacaaccac gaagaccgat atggttcttg
     2581 gaaaactttc tccacccttt ttgtaattgg agggatagtg gtttattgat agcgattttc
     2641 caagttcttc aagtggaacc agacattgag caccacggct aaaaagtgaa tgaagtcttt
     2701 atttttaatc aataattttt aatgattacc agtaattggt tttttcatgc gccctatttc
     2761 tatttgatgt ggagttaaat aatgctgata agtaaaaatt tttccgtcaa ttaaactgcc
     2821 actagtgaat cctgttctat tggtaaaaag agaatagtct gggtattcat ctacaccaaa
     2881 tgataaagat tcagccataa actgtttttt accagcaaat tcagcactgc taccttgatg
     2941 gatatgtcct acaaaaatac ctaaaatatc actttcggca atcaattcag caaaaccttc
     3001 cggaacttca gttacaatag atggatcttc ccacgacaat ggatgatgaa ccaataaaat
     3061 cgttcccttt tcagcaggtg tacccaacca ctcttgtaat tgaatcatct gtcctatcga
     3121 tatttttcct tcgtgctggg tatcttgagc agaatccaag gaaataattc gtaacccatt
     3181 ttgattcgta caatggatat actctcccgt ttcattgctt tcaacactca ttccttcaaa
     3241 gaaaacactg cgacgatcat gattcccacg acaaaaatag taaggtgtgg ttgggaaata
     3301 ttgatccaac attttccgta agacttgata atcagaaatc cccccttcat gtactaaatc
     3361 acctgttaat agaataaaat cgtacactgt taacgagagt tcttgtaaca atttttccaa
     3421 tgccaactgt ggatttttaa ttgttggaaa tagttcagca gtaaacaagt tagtttgata
     3481 gttttctcta aaatggatat cagagagatg caaaaagcgc atggctattc cctctttctt
     3541 caataaaata atcaggatat cctaaatttt cttgcgtctg ataatcaaaa aaatgcattc
     3601 tttcaggttc tatgcggaga ttgactgcat caccaatctg ccaagtaggt ttttcattaa
     3661 ggacaatcat ttctatacca tcgactttaa cggtgtacgc ataactttga cctaatagct
     3721 cacgatactt cacttgaccg tgtaaatcgc ttgtttcttt tgaaaaggtt acgtgttctg
     3781 gtcttatgcc taaatacaat gagccactaa cctgaagaag ttgtttttgc caagctggat
     3841 tcaatgttaa aaattgactg gctaccttta atgttccttg atcataagta actggcataa
     3901 tattcatgcc tggcgaacca ataaaatttg cagtaaaagt atttttaggt ttcgtataaa
     3961 catttttcgg cgtatcaatc atctgaattc taccttcatt caataaaaca atccggtcag
     4021 ctaacgtcat cgcttcaatt tgatcatgag tgacgtagac aaaagtttgt ttataacgtt
     4081 gatgaatttt taccaattct tttcgtgcat ccaatcgtaa acgcacatcc aaatttgata
     4141 acggttcatc taataaaaaa taattactcc gtttaaccac cgcacgagcc aatgctacac
     4201 gctgccgttg ccctcctgac aaatctttag gaagtcgatc ttggtatggc gttaaaccta
     4261 acatcttcaa agcttcttcc aaacggtgct gaatgtcagc tgtcttaatt ttattcgccc
     4321 ttaatgcaaa aataatattc tcctttacag tcatgtgagg atataaagca taattttgaa
     4381 aaaccattgc gatctctcgt tctccacaag gtagcttatt tgctagttgt gtacctaaaa
     4441 aaagctctcc actcgtaatt ttttccaaac cagcaatcat tctcagcaaa gtagattttc
     4501 ctgaaccaga tggtcccagt aacacaattc gttctccagc ggccacagct aaatcaattt
     4561 cttttaaaat ggtatgttct ccatattttt ttccgatatt tcttaattca agcgtttctc
     4621 ccatgatttg tcctccaatt tatttaatac ctgcagaaac aaaagtcccg ataatatatt
     4681 tttgaaaaat tgcatacaag actaacggta caaacattgt aattaaacta atcgccattg
     4741 ctacggtaaa atttgtccca ccttcagaac taatataggt ctgcatcgcc aacggcaaag
     4801 tataattatc tactgtccgt aaaattaata ccggccaaac atactcattc caagaggtaa
     4861 taaaaaacca aataccggta gaagtaatcg catgtctagt aagaggaaaa acaattttac
     4921 tcatgacggt tgtttgtttg atgttatcca atgccgccac atctagaagg ctcactggaa
     4981 tttcccgcat tgtttgcgtc aataaaaaaa tgcccatcgc atcagcaaat tgtggtatta
     5041 tcacacccca agtagtatca attaaattaa gttttgcaat caacaaataa ttaggaatca
     5101 tcgcaacagt aaaaggaatg aacatcgtac caatgatgat aaagtaccag attttcttcc
     5161 ctttgattcg gcaataagta aaactatatg ccgcaaaaaa agctacgcct aacttaacaa
     5221 ccgttataat tgtagcaata aaaaaagtat ttctcgtaat tttgataatt ggaagatttt
     5281 cgaacaaata gcaaaaattt tccaaggtaa acggctgtgg tatcaaacta gtaatcgtct
     5341 gataggcatc tttcaatggc ttaaaagaat tggccataat aaacaataat ggatagataa
     5401 taatgattat gatgaacata aataagagat gtggcaacca gcgtttaatt ttcatagtaa
     5461 accccctttt ccacaaattt aaatattacc cctaagaaaa cagcaaatat taataaagtt
     5521 aaaactgaaa ttgctgaaga ttctccgact ttatacattt caaaagcctt ttgataagca
     5581 taatagatca cgtttgcact accattattc ggtccaccac gagtgatgat cttaatcgga
     5641 gtaaacacat actgcagacc ttgaacaatg gtcatcacca ataaatatag tcctgtcgaa
     5701 ctactcatag gcaacacaat tgtaaaaaaa atgcgccaga tgggtacacg atccaactta
     5761 gctgcttcga tcatctcttg atcaattccc acaattgctg caaaaatgac aatgaaatta
     5821 taaccaaaaa ctttccaagc agtaatacct gcaatcacag caattaccca accttgtaaa
     5881 ttcatccaca taggcagtct taaaccaaaa tttcccaaca agacagcaat tggtcccgat
     5941 accggattta atatccatgt aaaaaccatc cccgccacta ccaaagaaat cacagctgga
     6001 ataaaaaata atggtttata caaattctgc cctttttcta taactattcc taataaaaag
     6061 gatgtaatat atgggaacac acagctcagc aagacaaata gaacggtata ggacaaagta
     6121 ttcttcaaca ctttatgaaa gactggatca gtcagcacct ttacatagtt ggcaaagcca
     6181 acaaattctt tagtgggggc caccatattc cagtcaaaaa aactcaaata gatcgtataa
     6241 atcaatggat aaaaaacaaa cacgcccaac aaaataattg ctggcgccag atacaaataa
     6301 cctacatagc gcttcatttc cgacagcctc ctcatcctta tgtcaaaagc agacgacctt
     6361 gtttttcctt tcttacaggc gctgtcgtct gcttgctttg cttatttgag taactcatta
     6421 atttcctttt gagtagcttt catcgtttca ttgacggaat tacctgataa aattttatcc
     6481 cgcatatcca acaatttttg ttcagcttct aatccagcat ctccaggaaa tgaactccaa
     6541 ctgaccatct tatccatctg atcaattgct ggtatcatca tctgattttc agctaagaaa
     6601 gcttttaatc cattttgggc gtctgcaaca tctttacgtg gtggtaaata accagtacct
     6661 tctgtccatt tagctaaaga ttcaacacta taaagatatt ttaagaaatc ccaagttgct
     6721 tcctttttct tatccgtttg tgatgtaaca gctaacattg cgccaccagc aggtagttta
     6781 acttttttcc cttcccaact tggtgactca actgctttag cagtaaaagt actatttttt
     6841 tgaatgttgt ccctttgagc aatagtggta tagaccatcg cgacgttacc atcaataaag
     6901 ctttgcacac cttgttccca cgtagtgtgt aatgccgtct ggtcttttac catcctttga
     6961 tataattcat aagcagcgat accatcttta gaagcaaagg tagctttgcc atcttttatt
     7021 acacttgccc cattactttc taaaattgct tgttgtgccc aattatcagc aggttcttga
     7081 atatacacgc catactttcc agttttttct tttattatct tagaaaaagt ttgaacttct
     7141 tcccaagttt ttggaccatt ttcatccaag cccgctgctt ttaatatatc ttgatttaaa
     7201 tataaaaccg gattactaat ggagtaagga atacccactt gtttaccatc tttttcagac
     7261 agtgccatga tattgtctaa aaaatgatct ttaaaataat cagcattatc cggatccact
     7321 tcttctacta aagattgagg attagtatat ttgaaatttt ctgcaaaata atctttaaaa
     7381 gcccatccca cttgtactac gtccggtatt tctttggagg caacagaagc ttgtaaattt
     7441 tgcatcaagc ctttatacat atcaggatta tattttgcta caaccttcac tttgtcttga
     7501 gattgattgt atttatctac taattcattc actgtttttc cacctagggt atccgcatta
     7561 acatgccaat actctatcgt tacacgaccc tccgattttt ttgttgtctt ggtattccca
     7621 ctaccacaag ctgccaaaga gatgacactt aaaacagaca caacccctat aacaagattt
     7681 ttctttaaca taacattaac ttcctcctaa tttctttatg gccatttttc taatcaagaa
     7741 atattatgaa ctaccaatgt aaatttctag ttaattttct gtaaataatt cgaaatttgt
     7801 gcatttcggg gtacagggtc actggacata tccatcaagc taagaataaa gcccccatat
     7861 ggtcatgtat cccatctcca aatctcttaa ctcctctgtt ttgattataa aatagtttat
     7921 tctgtgctcc tttcttccat aaataggccc atgcacaacg tgaggttata ttataaacac
     7981 cacagcgcgc aaggtactca atcgtttcta gttcattgac tgtccataca gaaaatgtca
     8041 atcctagctg ctgccccact tcacgggcca acttagttaa ccatccttca tgaatattga
     8101 cgatttgaac gctttgatag gctaatttct gtaaggtatc gatcaaatct gcttcggtaa
     8161 tatccccaag ttttttccct tcgaactgat tttcaatatt ataaaatata tgctttcccc
     8221 aaatcgcagc ccaagataat tgtacatagc cactaagaac aatttgtggc cagatttgat
     8281 aggtttgtgc cagcgaaaaa acggccattt ccaatccctc ttcttttaaa tcacagttaa
     8341 tggtcatggt tgccttctca ttggccactg cttgaaacac ctgctcaagg gttaccgaat
     8401 ctgggacaac ttcttacctg tcatgggcta gtattagcgt accatctgtt tgctgacgca
     8461 catctacttc aaaacaaagc tggcgatcca aacaaaagcg aagaaatgcc cagctattct
     8521 cctccgtatt gtcactacca gagtgtgcag tgatcatggt cattttgacc cctcctttaa
     8581 ttccgtgatt ttctcccaca cgtccactat accgatgaag cgttaatttc ttgttaattt
     8641 cttgtaaatt ccctgcgttt tttcagtaaa ctagttgtaa atcttgcatg ctccttccta
     8701 cgaactcatg aatcgtcgat tttgcccttc tagctagttt caccaattaa gtagttctac
     8761 attccccgct tttaacctac tacacctttt tttgttttca gctattcctt cttcttgctg
     8821 aaagtccttg aggttctttc ttacaggata ccatcaaggt gttcaatggc ttattcccat
     8881 tgaatgaccg agacttacta tcagatttct gctcgttgaa ccaagtcttt gatacaggat
     8941 ttcttccgtc atcttctcac aaataccacc ttgcgtttct ctagcgattg gtcgatgtgc
     9001 accctcccag tggactgtca ctaccccttt ttgctatgcc tagcccacaa aaaaagctgt
     9061 cctcacagac ag
//
LOCUS       DARBFO010000099         8944 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000099 DARBFO010000000
VERSION     DARBFO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.101"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(342..698)
                     /locus_tag="SCK51_002469"
     CDS             complement(342..698)
                     /locus_tag="SCK51_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEG1253044.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(712..1122)
                     /gene="spx"
                     /locus_tag="SCK51_002470"
     CDS             complement(712..1122)
                     /gene="spx"
                     /locus_tag="SCK51_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEG1253045.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1469..3655)
                     /locus_tag="SCK51_002471"
     CDS             complement(1469..3655)
                     /locus_tag="SCK51_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEG1253046.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            3873..4556
                     /locus_tag="SCK51_002472"
     CDS             3873..4556
                     /locus_tag="SCK51_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEG1253047.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4637..5356)
                     /locus_tag="SCK51_002473"
     CDS             complement(4637..5356)
                     /locus_tag="SCK51_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG1253048.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(5668..5874)
                     /locus_tag="SCK51_002474"
     CDS             complement(5668..5874)
                     /locus_tag="SCK51_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253049.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6119..6721
                     /locus_tag="SCK51_002475"
     CDS             6119..6721
                     /locus_tag="SCK51_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEG1253050.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            6862..8142
                     /locus_tag="SCK51_002476"
     CDS             6862..8142
                     /locus_tag="SCK51_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1253051.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8155..8883
                     /locus_tag="SCK51_002477"
     CDS             8155..8883
                     /locus_tag="SCK51_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1253052.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
BASE COUNT         2598 a         1673 c         1470 g         3203 t
ORIGIN      
        1 gatagaatca agcttcgcaa gtggttcttt tttaacaact cagttatctt atcatgtcat
       61 ttctaacatg tcaacaacta ttttatatgt tttttaaaat actattttat tgctttgcta
      121 cttaaaacaa cagaaatgaa tatatcatgt aaatttacac atgtcaatat atttttaact
      181 ctatatcccc acctagattt gaattttgac cacataaatt gagcatgtct ctatagtaga
      241 aaaataaagt caaaaaaaga acacataaca cgcttgatgc taagtgttcg actgttacaa
      301 ctgtatgaaa tgctcagtga cttgattaaa acagttaaag tctatttatt taagataagc
      361 tgttccatca actgctcatt gtatccaata aatagaactt catcgataat aatcaaagga
      421 cgtctgagga gattggtatg ttgtgtgcat aaaaaaatca gttgtttttt tcctacacag
      481 gaaaggtcaa tcgcaatgtt gtgaaaagcc tcagaccaag tcgcaaatag ctgttctgct
      541 tgcacttctt caaaactgca taattcttct gcttcttctg tacttagatc gcgttcttct
      601 aagtcaacaa attgataagg caattgatgg tgttctaacc attgtttcgc cttcagactt
      661 gttacacagt gcgctgatcc atagactttc acttccacga aggctccctc cctatgaatt
      721 taagatgtta ttgttttgta aatagatcag atttctggca acacatcgaa tttcacgagg
      781 gacaaatgac cggatctcat cttcattaaa tccgattagt attctttttt catcaatcaa
      841 aataggtcgt ctcaataatg aaggatattc tttgaataag acgatcattt gctttaaagt
      901 taattcgtta aaatctatgc ccagcttttc gtaaattttc gaccgagtgg aaataatatc
      961 ttgaactcct ccttcagtcg ctgccaagat ctttttgatc tcactttctg ttaacggttc
     1021 ggcaaaaata ttcttctctt caaattcaag ttgattcgct tgcaaccatg ctcttgcttt
     1081 tctacaggaa gtacagctag gcgaagtata tacttttatc attttctcac tcatttttaa
     1141 ttataatcca attctacagt aatttctttt aaaataccaa ttattagcta ataatatata
     1201 aaaaaaaatc aaaataactg attatttacg tttgaataat gagaattttt ctatatattc
     1261 tctattttga taatagtatt ctcattttca aaataaataa gggaggtgtg acaaaattct
     1321 tgtcacacct cccttttccg aataaactgt gttccagaaa tagcttcttc gaaaataagc
     1381 tgaaatttca caaaaatttg agaagcaatt ttcgtacatt ccttcttctt tcttgaaact
     1441 gaccacttct gtcacaccct ctcttctatc actttccttt tttcacacga ctcccggttt
     1501 ctttgataaa tctcgatact ttcaattttc ttgaattttt catttgaggt acacatagat
     1561 acagacgatt tcttgctcta gtcatcgcaa cataaaatag tcttcgttct tcttctatcg
     1621 tcgtcactgg ttctgctttt atttcattaa agtcgattac tttcttttct gtcgctccgt
     1681 tataaggcaa acttccttcg ttacaaccaa tcaaaaagac atttcggtaa ctaagacctt
     1741 tacttttgtg aatgctctgg atacatagtg catcaagttt tccttgtctg taagcaatca
     1801 agcgggtttt taattcttta gctgtcgctt gggtttcttg cgataaggct tggagtcttc
     1861 tgaaagtagc atgctgcttt acttcttctc gtatccattc ttgatcactc tggtcgattt
     1921 ccttagctat acgtggcaga tgaagatagc tttgaacaag cgaatagatt tcttgatgct
     1981 ggtctgtttt acgcaacgct tcctcaaact ctcggatttc tgcagatttc gattttaaat
     2041 tggggcttat cgacaaaata taatctgcaa cggctatatt ttgatattta ggagaaaagt
     2101 attgatcgca ttcacttttt ttcaggtagc agtctgccat cagctgccgg agcaattgct
     2161 ggtaggcctg atttttcttt tgcagattca gtgctgtttg ccaatgaaca attttttgat
     2221 agaaatgttg gaaagacttg ataccaaaga taccttcata tggactatct gcttccagta
     2281 caaaaggaat attcgttttc agcagagatt ggacaagctg ctgcgtttgt gaagctgatc
     2341 ggtataatat gattgattcc ttatatttcc catttttttg aataaatgtc tgaatttgtt
     2401 tgattagcca ctgactttct gctttagtat cagaaaacaa ataggcttcc ataacacctg
     2461 cttctttttg tgctggaatc atcgcctttg gtatccgatc atttttatta tagctgatca
     2521 atctatttgc tccttggaca atagctgccc gagaacgata attagtcaat aagaaaagag
     2581 ttttggtagg atattctttt tcaaagttct tgatgtatgc tggctctgat cctctgaatc
     2641 catagatact ctgatcatca tctccaacaa caatcaactt gatcctgttt tcctttgtca
     2701 tccgtttcac gatttcccat tgaagtggat tagtatcttg aaattcatca atgaatagat
     2761 aatcatattt ttggccgata taatgctgga tctcctcatt ctccaaagcg cttttcagat
     2821 tttggagtac atcattgaac tctagcaatt gctggcttct tttttgttct tggagttccg
     2881 tgagcaatcc atacagtcgt tctgatatcg gctgtcgtgc attcttatca atggaatcat
     2941 atttttcttt aactaactga accatttctt ctactgaaag gttcatattc tgccaatagt
     3001 ctaaccgaga agcaatcgtt ttttctccat attgatcaaa tatataaaga ttatatttac
     3061 gtatcagctg ttgcaaaaca gcattcactt tttgtgcagt atcctttgaa aagtctcctt
     3121 gtattccttg cattgctact tcaggaaatt ttcgacaaat ttcattgtat atcctaaaaa
     3181 agatactatg gaatgttccg atatggacag cggtttttct ctggctgaaa ttatctactc
     3241 gtttttttag ttcatttgca gcgttccttg taaaagtgat cattagtatg cgttcttcgt
     3301 ctacttgttt ttgcgtcatc agataaacag cttttgcaca gattgtttgc gttttgccgc
     3361 tcccagctcc tgctatgatt cgaagatgtt tcttcatatc atatgtgata gctgcttttt
     3421 gttcttgatt gaagcgatta taggaacgtt tctccatcat tttaaagaaa gagctgtttc
     3481 cttcactttc ttttacttgt acttcttcct caataagtcc gggtgcttcg ccataagccg
     3541 cttcataggt agcaatgatt tcttcttgta gccgaaagac ttgttgaagc tttttatact
     3601 tgttctccca gcgtttcaat tctttttgta atcggagaat ttctttttca ttcaactgat
     3661 tgatttcctt cccatctttc ttctttgaat ttgtttattt caagttggtt atttaaagtt
     3721 tcgttgtcta ttttttcttt atactaacag acctttctaa aaatgaaaat gggaagcgat
     3781 atgattttag ttttctgaaa atttttcctt tttttcaatc gtttatgcta agataaggaa
     3841 gacaaaaata ttcataaaag gtgatgaaaa aaatgcagac ttcctttcac gaggggcgta
     3901 caataggact tttgctgaca tttatcggag gagccatgga cagttatact tatatccaat
     3961 atggtgcttt tgcctctgct caaactggca atatcattct atcgatcatc caagcatttg
     4021 acggagaatg gctaagtgtt ggtaaaaaag tattgtctac ccttttcttt ttcttaggga
     4081 ttcttctaac aaagtatcta attgattatt ttagaaaaaa agaaaaacat ttctggcgct
     4141 tgtttgttct atattatgaa gcagtgattt ttttctttgt tagtttgaca cccgtccatg
     4201 agcatcccgc attagtaacg attttgattg cttttactgc atcgatccag tgggtagctt
     4261 ttgataaaat caacgggcgt gcctacacta atttatttac aactggaaat ttaaaagggg
     4321 tagcaacgaa cttatatgat tattgtattt ctaaagaaaa agcagatttt gatcgatttt
     4381 tccactatct aagtgtcgtt cttgccttta tcgcaggagc tattgtcctt gttttctgtc
     4441 atcatctgtt tggcgcccat tcgattttga tcgtttccat tttatttttg atcatggccc
     4501 tcactcagac atttcattta tggcaatttt atcatacgaa tcggttcact atttgatctc
     4561 aatgaatcaa aaaaactaag caagatgctg ctcacagaga aatatctgcg agtgagcaat
     4621 ttgcttagtt tttatattat ttatttgtaa agattccctt tgctttcggg ttatttgtag
     4681 tgtagcggat cgactgggca tctgcctctt ctgtcatttt ctttgcccgc atcttgattt
     4741 cttgtacgat ttgttctcca catgccggat cggctacgaa atcatatttg tccccatcga
     4801 tcactatttt gggacatata tcaaactgtt cataccactc ttcgtatcgt cgattgagtt
     4861 ctttatagta ggaatagagt tcaggatctt gttctagctg ttcatattcc cggcctcttt
     4921 ttttgattct cgctaacata gtttcaaagg atacacgaat ataaaccagt agatctggtt
     4981 tcttttgtgg cgaaatttct tctaactcct tcagcatcgt ttctaaaaga ccttcatact
     5041 gctttacttc tacttcagaa acacgcccta aatctgcatt caaatgaaac agcatactat
     5101 cttcgtagat ggagcggtct agtacgttgt catcatggct gaaagcatct ttgattgcca
     5161 aaaagcgttt gttcaaaaag aaaatctgta ataaaaaagt atacttttct ggattagaat
     5221 aaaatagcgg caatacttca ttatcttcca cgttctcata aaaaggtttt gtttctaaat
     5281 cttgggcaag catttcagtt agtgtgcttt tccctgcacc gatcgttcct gcaacaatga
     5341 tcacaattat cggcatcctt cctgtaataa aatctgttct tcattttact acttcattta
     5401 tagaaataaa agccttgact ttatctgctc cttaaaagct acttctctaa aagtagccaa
     5461 aagtaactcc tattcatgaa aataaaggct gtgacaaaag tcttgtcgca gccctttttt
     5521 caaataaaca gtattctcct gccaacacct cggtcacaag gcaatattca taaaacatga
     5581 aaagctgttt tttaacatta ttccttgtaa ctcagagcta aacgggagac tcacaacctc
     5641 tttatgctct caacatttta tctgttttta ttcttctatt gtacttgatt tgcgtacaac
     5701 taactgccag tgcagattat gagcggtatc attgtcttct actgcgcatt tcatgacacg
     5761 tatcatcaca ctattttcat agattttttc tatttttcct tttattggtg attcaaataa
     5821 atcagattta aatgcatatg tttcgccgac ttcaaacaat tgttcatttt ccataagaat
     5881 ccctccatca ccttttataa ggtcagtaat tttttaacat aaatcactcg aaaaagcaca
     5941 aacttgtcat aaaaagtact tttcgtatca aaactgtcac tttttttggt aaggttcttg
     6001 gaaaacgaag atttttttag gagatcatga cggttttgat gtcaaaacca ctttttatga
     6061 aacaaaagac gttttttatt ttttttagca taaaatttgt ggtggaggta cgtatgagat
     6121 gaaagataaa gttgtgaaca aattattaga agccttattc agcggagaca aatcggaaga
     6181 agatatggtg tatgtccaag taaaatttgc cttagaagtt ttattgaata atcttggaaa
     6241 actagcggtc gttctagtct tttctctggt gacaggctca tgggcggaaa caggaatcac
     6301 ctttctttct tatatctgca tccgcagata tgcttatgga ttgcattccg actctgaatt
     6361 cgtttgtctg ctgtggacac tactttattt atggggcgtt cccttggtga tgaaacatct
     6421 gcaattgact atctccttcc caatgatggt ctttttgtta atcagctgct ttttgttact
     6481 gcttcgctat ggatcaagag gtacagcaat caatcccatt gaacccgaga aacgtccccc
     6541 attattaaag aaggcgatca gcatgtttct aattttttct ctcattactt tatttttctc
     6601 agcttcttac ttttctacct atttgctact tgggattgtt ttagaaatag ctacgttgct
     6661 tcctattaca aactacttga tgaattttgg aggaatcttt catgaaaaaa acaatcatta
     6721 aacaattaac taacttatct gacttttttg tatctggctg ctgctttggt atgagttctg
     6781 aattcaaaat ccctgaaaaa ttaaaaaaat aatacgctaa ttgtgctata atttggtaaa
     6841 aatcaatggg agctgtactt tatgttttta tggtccatag cgttgcaata tttgatttac
     6901 acaatttgta tcttattgat tgaccatcac atccgataca aatatctgtt tgcgggagtc
     6961 attctgattt cttgtatttt gattggatgg gtctattcga ttttagggat ttttactgct
     7021 ctaattgcta taggaatgct gatgcttact tttttcttct tttcgacttc gcgtcaacaa
     7081 ttggtttacg cgcttccttt tgggttattg acgtcgcttt taggagatca cttaacaagc
     7141 atcgacgatt tttttgtttt gaaatcttcg gttattgaac caggagattc attgcagtat
     7201 tttcacattg tcttatccgc ttttttatct gctttattag ctttttgttt aaaaaaatcg
     7261 ttagagactt ggtttcacac tgtggaccgc cgtatgatcg gtacgatcgg gacattgata
     7321 ttatttacat actatatcat tattttttat acccgttttt ctggtgaaac acctgatgtc
     7381 ttagctttga atacgagttt tttcattcta tatctgtgtg ttggcctgat catcttggtt
     7441 tattattgga aaatctctaa taagaaatta gctgatcagt tattagagca acaagtcagg
     7501 atgcaacagg attatatcaa agatctagaa aaaaactatc aagacttgag agaattcaaa
     7561 catgattacc aaaatctgct tttttctctt cacagctata tttcagaagg cgacttagaa
     7621 ggattacggt cttattatga tcaaacgata ttacctacac gccagatgat gaaccttttt
     7681 ccggccaatc taagtatgtt ggataatatg aaagttcctg agatccgtag tttactgtca
     7741 ttaaaactca tgatggcaca ggaaaaaggg ttgatcgtcc agcttcattt tcctgagcca
     7801 atcgaactgg atacgaaaca tacggtaaac cttgtacgta tgcttggtat tatcttggac
     7861 aatgcgattg aaggagctag tgctgcaaag aagaaaaaaa tcgaattgac catactcaag
     7921 aagaatcatt ccgtgatgat tcgtgtgatc aatacaacaa caaatacatt accgttaaac
     7981 cagttgaaac aaaaagggtt ctcaacaaaa acaaatccaa aaaacgaagg ccttggcttg
     8041 tttatcttgg atgaattgac aaagaccaat ccttatcttt tccttgaaac atcaattgaa
     8101 aaccagcgtt tttctcaaac gatttatata caaataaaat aaaggagtaa tgatatgatt
     8161 cccatttata tttgtgaaga cgatccaaaa caattagaag ttttatcaac ggtgatcaaa
     8221 aatagaatca tgattgaaaa cttagacagt tatgtccata tttctacctc tgatccggaa
     8281 gaacttctca atgcagccaa gatcagaact tcttcttttg gtatttactt tttagatatc
     8341 gaactagaag acagtgaaat ggatggtgta gcgatcggta aattgatccg tgaattagat
     8401 cctttaggaa aaataatcta tgtgacttcc catacggaaa tgtcgatgaa aatcttaaaa
     8461 agcaacatcg aaccgactga ttatatcgtc aaagaagatc tttatgattt aaaagaaaat
     8521 gtcgagcata tcctgagcaa aattttcgaa gattttcaaa tgtcccaaat ccaaaaagat
     8581 atcttcaaaa tagagtttaa tgatgagatc aaatttttac cgatcaaaga catccaatat
     8641 ttttctactt cgctaggagc gccacataaa ctggaagtcc atttgactca tgcccaatta
     8701 caattctatg agaagatcaa agaaatcgag aaaatgcatc ggaattttat ccgttgtcat
     8761 aaatcctatg ttatcaatac acaaaatgtg cggtcgatta ataaaaaaac cagagaagtc
     8821 acgttggcaa acggcgagac catcccagcc tctatccgtg gattaaagaa attgatttca
     8881 taaaaagaac cgaatagttt ggaccgacta aatgaccagc cgatccaaac ccttcggttc
     8941 tttt
//
LOCUS       DARBFO010000100         8900 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000100 DARBFO010000000
VERSION     DARBFO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.102"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(254..2146)
                     /locus_tag="SCK51_002478"
     CDS             complement(254..2146)
                     /locus_tag="SCK51_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1253053.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2143..3870)
                     /locus_tag="SCK51_002479"
     CDS             complement(2143..3870)
                     /locus_tag="SCK51_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1253054.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3886..4536)
                     /locus_tag="SCK51_002480"
     CDS             complement(3886..4536)
                     /locus_tag="SCK51_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEG1253055.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4740..5036
                     /locus_tag="SCK51_002481"
     CDS             4740..5036
                     /locus_tag="SCK51_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG1253056.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5208..5828
                     /locus_tag="SCK51_002482"
     CDS             5208..5828
                     /locus_tag="SCK51_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEG1253057.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5920..7086)
                     /locus_tag="SCK51_002483"
     CDS             complement(5920..7086)
                     /locus_tag="SCK51_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1253058.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7098..7451)
                     /locus_tag="SCK51_002484"
     CDS             complement(7098..7451)
                     /locus_tag="SCK51_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEG1253059.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7579..8601
                     /locus_tag="SCK51_002485"
     CDS             7579..8601
                     /locus_tag="SCK51_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEG1253060.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8620..8781
                     /locus_tag="SCK51_002486"
     CDS             8620..8781
                     /locus_tag="SCK51_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253061.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
BASE COUNT         2700 a         1827 c         1624 g         2749 t
ORIGIN      
        1 tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
       61 taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagataca aaacggaaaa
      121 atagcttctc atattttgcc gttttttgag ctaataccga aggacagctg attgaacacc
      181 gtttattcgg aaaaagggac tgtgacacga cttttgtcac agcccctaaa aaaaataata
      241 aatttgtact attctatgaa gcttcttctt cagaaaactg actgttataa agatctgcat
      301 agaatccgtc tttttccatc aatgtatcgt ggtttcctgt ttctacgatc gaaccatgat
      361 tcatcacaat aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca acaacgaaac
      421 ttgtccggtt ttccaatagg cggttcatcg ctttttgaat cagtatttct gttcgtgtat
      481 ctacactaga tgttgcttca tcaaggatca ggacatctgg atttgctaag aatgcccgcg
      541 caattgtgat tagctgacgc tgcccttgcg aaatattgct tgcatcctcg tttaatacag
      601 tgttatatcc ttctggcaat ttacgaacaa aatcgtctac gtgagcagct ttagcagcct
      661 caatgatttg ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca tagatacttc
      721 ctgtaaacag ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat tcatcacgtg
      781 aaagatcacg ggtatctaca ccatcgtaac gaatactacc tccagaaaca tcgtagaacc
      841 gttcaagaag gttgatcagt gtcgtcttcc ctgcaccagt cggacctaca atagcgacca
      901 tctcaccagg tttgacatcc aagttaaaat cagtcattag caagttatct ggtgtgtagc
      961 cgaattgaac attttcaaaa cgaactttgt atggtgtatc tttttcttct ggaaggccag
     1021 atttttcatt ggacatctct tcttcatcca acacttcaaa tacccgttcg gctgaagcaa
     1081 ctgttgattg gatagtgttc atcaaacttg caagttgagt gattggttga gagaactgtg
     1141 ttgtatattg caagaacgct tgaacatccc ctaatgtcat gttcccattt gcaactttta
     1201 ctccgcctag tacagcgacg aacacatagc ctagattttt aacgaaattc atcaacggca
     1261 tgatgattgc cgaaataaat tgagctttcc aaccagcggc atacagattc tgattttctt
     1321 tttcaaatac ttcctggtct ttttcagtat gattgaatgt tttaacaatg gtatgaccac
     1381 catatgtttc ttctacttgg ttattcaata aacctaaact tttttgctga tctgcgaaaa
     1441 acacttgtga tttcggcgct acgatcatca cgatgatcaa gcttaaaggg atcatcaata
     1501 acgcgactaa cgtcaattgc cagctgattg aaagcatcat cacgatgacc ccaatcaatg
     1561 tgactgcact agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg atgttatcca
     1621 tatcattgat tgctcgagac atgatatccc catttgtatg tgtatcataa taggaaatcg
     1681 gtacacgatt cattttggct tccaaatcat gacgtaattc ataaactgtt cgttgggaaa
     1741 cacgtgtcat aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa tacatcacga
     1801 ttactgtcgc aatgatctgc gcgattttat caaagtctat cggaaatttt gttacttgtt
     1861 ggccttgttg catctgttga gcgccagcca ttacaccttt gaatatttct gtggttgctt
     1921 tccccaagac ctttggtgtt tggatttgga agatcacggc tgctattgct agtacaaata
     1981 cagcgatgat cgcaaccata cgtttagaca tatagccaaa caatcggcgt accgttcccc
     2041 agaaattttt aggcttttca ccttttacac cgagatttct gccaggtcca ccatgtcctg
     2101 gaccgcctgc tggtccatga gagggacgtg tgtttgtttt actcatgcta agtcctcctc
     2161 tcttagttgt gaatgaacga tttcttgata agtttcgttt gttgctaata gttcttcatg
     2221 tgttcctttg ccaaccagtt ttccttcatc tagcacaagg atcatatctg catccattac
     2281 tgtacttact cgttgagcaa ctaaaacggt gatactttct ttcatattcg ttttcaatgc
     2341 ttggcgaaga ttggcatccg ttttgaaatc caacgctgag aaggaatcat caaacacata
     2401 gatatctgct gttttgacta atgcccgtgc aattgccaga cgctgtcttt gtccaccaga
     2461 gaagtttcct cctccttgtt ccacatggct atctaagcca tcttccatct ctgaaacaaa
     2521 atctttagct tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg catccggctt
     2581 accatactgc atattgtctc ggatcgttcc agagaacaga actgcttttt gaggaacaaa
     2641 gcccattaat tctcgcaaat tatattgagt catgtcacta atgtccgttc catttatttg
     2701 gatcgaacca gattcgatgt catataatcg agggatcaaa ttgactaagg tcgttttacc
     2761 tgaaccagtc ccccctatga ttgcgacgat ctcgcccgac ttggcttgga aatcaatatc
     2821 ttccaatgca agtttttctg ctccatgata tcgataattg acatgattga aggccaatgt
     2881 tgcgtttttt ccagcaaaag aaactgtttt aggattttca gggtcatgga taccgatttt
     2941 ttcatccaac acttcattaa tacggtcagc tgaagcttgt gcacgaggca ccatgataaa
     3001 aatagcggaa agcatcataa agctgatcaa gatctgcatc gcataagtca taaatgcaat
     3061 cagattaccg acttccaaag tcatatctgc tatgtagtgt ccaccaaacc aagtgattgc
     3121 tacgtttgta ccgctcatga tcaatgtcat cattggcatc atcaatgcga cgatcgtatt
     3181 aactttgatt gccgtatttg tataatcttt attcgctaaa tcaaaacgat tttcttcaaa
     3241 gcgtgtctta tcgaatgctc gaatcactct gacccctgtc aatccttctc gaaataccag
     3301 gtttaaacgg tcagttttct tttgcatact tttgaataat ggaacagcaa agtacatgat
     3361 tccgccgatc aaaataatca ttactgggat gacaactaag aagatttttg tcaactgatg
     3421 atctttttga taagcaagta tgctcgcgcc aatcaacgtg attggtgcat tgatcatcat
     3481 acgtaaaaac atttgtgtaa ccatttggat ttgagtcaca tcattggtcg ttcttgtgat
     3541 caaagaggcc gttccatatt tatcaaacgc atcttttgtc agactctctg attttttata
     3601 gacatccgta cgcaattgct tacccagttt ctgtgattca cgagtagcaa aaaatgtatt
     3661 tgcgatagca gctaagatac tgatcaaaga aaagccgatc atcacaaaac ctgtatgcca
     3721 aatataatcg acatctcctt gcgctacccc tttatcaata atattggagg taagtgtcgg
     3781 caaatataaa tctgccagta cttggatcac catgaaaact gcagctgcaa ttgctgaagt
     3841 caaggagatc cgttttacga tttttagcat ctagttccct tctttctatt tatatactcc
     3901 gtttttcagc caagaaagtt tcaaattgaa ttcactgatt gcttgctcta ctgagtaatc
     3961 ttcagatgtt gaaaataagc gatacgaatg cgcaattgtt gtaaatacag tattcatcac
     4021 taattggatc agtaattcca attcatgatc attttttact ttcagcttcg tccgatcgat
     4081 caattcaagc aactcttttg catcttgttt gtgttcttta tgtggatcgt gaggcgtttt
     4141 ttggcgactc gtatgaggag cgacttttct tgaggcacgg taatccatgt tcataaacaa
     4201 gttacgataa aaagaagcat tctcgccttc caaaatctca taaatcattt tagaaaaata
     4261 ggattcgaat cctttgaata gatcaccatc ggattctttc attagttgta acatatcttt
     4321 agagctgtca cgtctcattg tctcaaagta ataaaaataa agatcctctt tatcttcaaa
     4381 atactgatag aaactgcctc tagggatatc cgctaatttg acgatattag caatcgaagc
     4441 ttcttttaag ggtactctag aaaactcgat tctcgcagcc tcgattagtc ttttttgttt
     4501 ttctttaggc aaatggaaaa atgtctgtgt cggcatatgg atcctccttt ctttcaagat
     4561 gacacattgt catatgacgc tgtgtcatta taacacctcc tacctgtttt gcaagacatt
     4621 tgcatagttt ttttcattat catccgatac attaaaaaag tgataagagg aatccttcca
     4681 tttgaattaa tgagagttgc ccattaaaat agccacatac taaacaaagg atgagataaa
     4741 tggcccaatc aattagtgtc caagacttca ataaattgcc tctcgatgct gacagcatcg
     4801 ttttagatac aagaaatacc ttagattaca caaaaaacca cttaagccac tcgctttcta
     4861 tccccgctgc tatcttgcca agacaattaa aaaatctaga tccaaagaaa ctttattata
     4921 ttctaagcta tacaggcagg cgatcagaag cattagccga tcaacttact gctaatggtt
     4981 ttcgtgctgt ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt gcttgaatat
     5041 tgcttgttag attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc ttttataccc
     5101 tgtctttttt tagttattta ttcttcaacc ttatcaatat gacttgatag ccttttcttt
     5161 ttctcatagt tataacaatc atagcaataa aaggagaagg taagcatatg gcatctgttt
     5221 tagtagtcaa aggtcatccc ctaactgcag aagaatcgcg cacagttaag gcattgacgt
     5281 cttttttaac aagttataaa gaaaatcatc cagatgatga agtgactgtt ttggaacttt
     5341 atcgcgatga tattccggaa atcgatgaag aattattatc aggttgggaa gcattacgtg
     5401 ctggtgcaga attcacttcc ttatcagaaa gtcaacaaca aaaaattgct cgtttcaatg
     5461 aattgactga tcagttcctt gctgctgata aaatcgttat cgcaaacgcc ttatggaatc
     5521 taaatatccc aacaaaatta aaagcttggt tcgataccgt caatgtagct ggaaaaacgt
     5581 tccgctatac agaaaacggg ccagaaggat tagtgacagg taaaaaagca ctgcacatcc
     5641 aatctaatgg cggtgtatac aacggacaag actttgcctc tcaatatgta aaaggtattt
     5701 taaactttgt aggcatcgat caagtagatc agctattcat cgaaggaatc gattatgacc
     5761 ctgaccgtgc agatgagttg atgcaaaatg cattagataa agctgctgct ttaggtaaat
     5821 cattttaaaa attgagataa atgcatacaa aagagacaga ggaaaaaatc ttcaaagcag
     5881 catttgtacc ttggatattt tcccatctgt ctctttttgt tattctttct gctttttcaa
     5941 cggattttct tcataaagag aagagattgt tgtgtaccat tcaaaaacat gagtaatcac
     6001 tactttagct gctgcataaa caggaatccc caagatcaca ccgatgattc cgaacaattt
     6061 cccagaagtc agcagaacta ataaaatcgt tactggatgg attgctaact gattccctag
     6121 tactaatgga gagatcaagc gtccttctat tgtctgctct actgcaaaaa cgatcagtac
     6181 tttgatgatc atgaatggac ctgctacaat ggctaaaaag atagccggaa tcatcgctaa
     6241 aaatgatcct aagtaaggga tcaggttcaa aaaacccgca ataatcccca acgtgacagc
     6301 atactctaaa ccaatgaccg caaaaccaat cataaacatg attgccacag caaaagccac
     6361 agttagttgt cctcttatat aagaagaaac ttgactattc atttcactca gcacattcaa
     6421 cgtcggtttt tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta attgtttacc
     6481 gtctttcaat agataaaata agataaacgg catcgtcagc agagccacta aaatagtcgc
     6541 taccgcgccg acaaaacttc caagactctg aactgtactc ttagatatat cttggatcat
     6601 atcgcctgcc gaattcatga acttatcacc tgctgtttcc aactgttcgc gaaattggtt
     6661 gaacagcggg tcccgcaaaa tctcggttaa tttattatca atcgtatcta catattgagg
     6721 gaagttactg atgaagctca ctgtttgttc ctgaatctta gggatgatca ctacgctccc
     6781 ccatgcgatt agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa gccttggtac
     6841 ttttcgcttt tcaaagtaat caactaccgg attcatcaag taatacaaga ttcctgctaa
     6901 aatgataggc agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca aatatgaaac
     6961 ttttgtaaat aaaaagacga tcagcaatat taacaataca acaagtaagg ctgtgactac
     7021 ttgattattt aaaaaccagc gccaaaacca cgataccggt ctgttcttct tattttttcc
     7081 ttccataatt cctcctgtta gttataaaaa atttggcttc ccaaaccaat agcaggcagt
     7141 ctatgcggat gaagatagat tccatttgca atttgtcctt cttctggaca agcatcaaaa
     7201 atcaatgata cgtgagctac tgtctctaaa ttcccattgg ccatcggacc gacataatcg
     7261 atcgtatata cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga aaaaggttcg
     7321 tcttttgata cttcttgaat caccgaatat tttcgtaagg cagctgttgc tgttttatcg
     7381 aacaaaatca gcatgggttc ttccttgttt attgcttctg tcccaatttc tgtaatcttt
     7441 gcctgaatca ttgcactctc tcctttaatt gctttcaaaa acagtataac acattctcat
     7501 tctttagccg tgtggaataa ttatgtgata gaataaaaac gaaaactatt tcattacaat
     7561 taggaggaat cagctcatat gatcgaaaag ataattttag gaacctatac gcgtcgcgaa
     7621 agtaaaggaa tctatacgat cgaccttgat acagaaaaag aagaattagc caacctttca
     7681 ttagctgttg aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg caatctttac
     7741 acagttacta gtgtggatgg attaggtggt gccggtgcgt acgataagga ttttcatttt
     7801 ttgaatgcgg taacagagtc tggcgctcct ttgtgttatg tagcggttga tgaagcacgt
     7861 caattactat acggggcgaa ctatcataaa ggtgaagtaa atgtctacca tatattagga
     7921 gatggcgaac tgaaagcagc agattcaata ttccatcaag aagcgacagg tcctcataaa
     7981 aaccaagatc atgcgcatgt ccactatacg gatctgactc ctgaccaacg attagtcgta
     8041 tgtgatcttg gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg caaattgaat
     8101 cttgtaacta ccttcactgc agagccagga acaggccctc gccatcttgt ctttcatcca
     8161 aatggacaat tcgcttattt atttggtgaa ttagacagca cagtccgcgt gctttcttat
     8221 aataaagaaa caggtgcttt tgaagaatta cagaaaatat cgaccttgcc agaagaattc
     8281 gacggtgaaa atggaggggc agctatccgc atttcaaatg atggcaagtt cctttatgct
     8341 tctaaccgag gacataactc gattgctgta ttcgcagttt ttgaagatgg ttcccacatt
     8401 caaaacaagc aaatcatctc aacagaaggg gattttcccc gcgatttcgc actagatcca
     8461 agcaataatt ttgtcgtgtg tgccaatcag aacaccgata atctgacatt atatcgccgt
     8521 catcctgaaa caggattact ggaattactg aaaaaagatg tctatgtacc agaatgtgtc
     8581 tgtgtttatt ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca agacgtatta
     8641 gacagactac gggaagaact aaatattcct ttctttgatg ggaaaatcga agataaagag
     8701 tatagcgaag aggaatatca gaaactcaaa gccgatttac agaactactt tgaacaatat
     8761 gtacagaatg tagaaaacta atcagctttc catactatct gaaaagaaca tcaggacaag
     8821 acttttccgt ctatcctgat gttcttttct acaaataaaa aaccgccgag atggttagac
     8881 tcgacggaaa aggagtattt
//
LOCUS       DARBFO010000101         8827 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000101 DARBFO010000000
VERSION     DARBFO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.104"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            81..659
                     /locus_tag="SCK51_002487"
     CDS             81..659
                     /locus_tag="SCK51_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEG1253062.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            663..1184
                     /locus_tag="SCK51_002488"
     CDS             663..1184
                     /locus_tag="SCK51_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG1253063.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(1263..1817)
                     /locus_tag="SCK51_002489"
     CDS             complement(1263..1817)
                     /locus_tag="SCK51_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG1253064.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            2070..2345
                     /gene="rpsP"
                     /locus_tag="SCK51_002490"
     CDS             2070..2345
                     /gene="rpsP"
                     /locus_tag="SCK51_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEG1253065.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            2357..2608
                     /locus_tag="SCK51_002491"
     CDS             2357..2608
                     /locus_tag="SCK51_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEG1253066.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            2733..3524
                     /locus_tag="SCK51_002492"
     CDS             2733..3524
                     /locus_tag="SCK51_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253067.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            3694..4215
                     /gene="rimM"
                     /locus_tag="SCK51_002493"
     CDS             3694..4215
                     /gene="rimM"
                     /locus_tag="SCK51_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEG1253068.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            4205..4966
                     /gene="trmD"
                     /locus_tag="SCK51_002494"
     CDS             4205..4966
                     /gene="trmD"
                     /locus_tag="SCK51_002494"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEG1253069.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            5102..5449
                     /gene="rplS"
                     /locus_tag="SCK51_002495"
     CDS             5102..5449
                     /gene="rplS"
                     /locus_tag="SCK51_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEG1253070.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(5581..6717)
                     /locus_tag="SCK51_002496"
     CDS             complement(5581..6717)
                     /locus_tag="SCK51_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1253071.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(6833..7747)
                     /locus_tag="SCK51_002497"
     CDS             complement(6833..7747)
                     /locus_tag="SCK51_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEG1253072.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(7838..8266)
                     /locus_tag="SCK51_002498"
     CDS             complement(7838..8266)
                     /locus_tag="SCK51_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEG1253073.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(8271..8645)
                     /locus_tag="SCK51_002499"
     CDS             complement(8271..8645)
                     /locus_tag="SCK51_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1253074.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
BASE COUNT         2861 a         1556 c         1618 g         2792 t
ORIGIN      
        1 aatgtctact gccggagctt ttctatcagc aatttttatt ctttactaat acaattactc
       61 taaacgaaga ggtggcacag atgagatgtc cgtggagtga acaaacagaa agtatgcggc
      121 aatatcatga tactgtctgg ggtgttcctg aatatgatga ccaaaagctg ttccgaaaat
      181 tgatgcttga tattaatcaa gccggattga gctggcagac gattttaaat aaaagtgaag
      241 cattcgatga agcatatgat cgattcgata tcaaaaaagt cgcaaattat ggagaaacaa
      301 aaatcagtga actaatggaa aacaaaggaa ttattcgtaa ccgaagaaaa atcgaagcag
      361 ccgtccaaaa cgccaaagca gtcttgaaaa ttcaagaaga aatcggcagt ttttcagagt
      421 atctttggtc gttttctaat ggaaaaatcg ttgattcttc ttatcattca caagaagaag
      481 tcccaacaac aagtcctctt tcagatgaat tagcaaaaga tctaaagaaa agaggattta
      541 aatttatcgg aagcacaaca atctatgctt ttttagaagc tgtcgggatc atcaatgatc
      601 accttgacag ttgtttccgc aaagaagagg tcgtacgtat cggcagaaag ggcgaataaa
      661 gcatgcgaaa aatcatattt tatggtgcta taagtttaga tggctatttg gcaacgaaaa
      721 cggatgatct tcaatggctt tttgacactc caacaggtaa gcaaactact tatgaagctt
      781 tttatgaaac aatcgatacg accatcatgg gcagaaaaac ttatcaggaa gcaaaaaaat
      841 atttggagac cgaagcaatt tatccagaga aaaagaattt cgttttttcc acccacgcac
      901 aattacaatt gccagatgct gaggtcgtaa acgaagatcc tgttgctttt ctaaaaaatc
      961 tacaaatgac agaaggaaaa gatatctggg ttgtgggtgg cggtcgattg atcaaaccgc
     1021 tattggaaca tcaaatgatt gatgaatggt atatccagat tacccctgta ttattagggg
     1081 acgggattcc tttattccaa aaaggaacat atgaagcgcg ttttgaaatg ctagacacga
     1141 cacgattcgg tgaatttatc gaattgcatt atgtacgaaa gtagaagtaa ataaaataaa
     1201 aaaggcaact atatgcagaa atgtcctgta catagttgcc ttttttatta ccgttatgct
     1261 ttttactctg ttaatttgtt agaaaaatct agatacgcat ctaaaatcgt ttggaagaaa
     1321 cttaatgttc cttcaacgat attaccattt tcatcaatta gattcgcaac gtttccgata
     1381 tatgcttccg gttgctgcaa ggttggcaca ttcaaaaaga caagtgattg gcgtagatgg
     1441 tggtttgcgc caaagccgcc gatcccacct ggtgatgcag taacgactaa acctggttta
     1501 tgatcccaaa cactgtgtcc ataaggtctt gagcctacat ctaaagcatt tttcaatact
     1561 gctggcacag aacggttgta ttcaggagta acgaaaacga caccgtctag tcccctcact
     1621 tcctcacgaa aacgagtcca ttcagcagga gcctgttctg gagtttcgag atcctcattg
     1681 taaaatggca gatcatcgat ttttacaaat acactttcgt acccttcagg aagcaatttc
     1741 gcaaaagctt ctgctacttt tttgttgtaa gaatcttttc ttaaactacc aataaaaaat
     1801 ccaatttttt ttgtcatacg atttttcctc cttcaaacta tctataataa tcttacaaaa
     1861 gataagtaac tggagcaaat aaaaacactt actgacaaaa gctacataga agatagatta
     1921 cttattttta acaaaaaaga agtctaaaat cttctatttt ctaaaaaaaa tcgaatttaa
     1981 tcaagctgta aagaaaaaaa gctttacagg gtaaaaaaaa actgttacac tacatcttgt
     2041 gaataaaaca atggaggtgg aaataaataa tggcagttaa aatccgttta aaacgtatgg
     2101 gttctaaaaa gagtccgttt taccgtatcg tcgtagctga ttcacgttct cctcgtgatg
     2161 gacgtttcat cgaaactgta ggtacttaca atcctttgaa agaccctgca gaagtagttt
     2221 taaaagaaga cttagttctt gactggttat caaaaggtgc acaaccttct gacactgtac
     2281 gtaacatcct ttcaaaagaa ggcgttatgc aaaaacacca cgaagctaaa ttctcaaaga
     2341 aataaggtga ccggatatga aagatttaag cgacttagtc ttaacaatcg ttcgtccgct
     2401 agtcacttat cctgatcaag tcaagttaga cgtcgtagag tcgaaagact tttacgagta
     2461 caatctaaca gtggctcctg aagatattgg ccgtatcatt ggaaaacaag ggcgtgttgc
     2521 taaagcaatc cgcacaatcg tttacggtgt tcggatcaat gcaccgaaaa aagtgcgttt
     2581 gaatatcatc gatgataagg aaaagtaaat aaacgtgctg tactcgaatt ttcgagtgca
     2641 gcttttttgt tttttttatt aattgaagaa atgattattt ggcaaaacaa gcaagaaagc
     2701 gtatccttat aggaggaggg agtgaacagc caatgagaaa gaaaatgtta tttattccat
     2761 ttgctctatt gttaagcgct tgtagttcgg ttactcaaga atcagacaat acacatctat
     2821 caagcagtat atctgaaact gttaccagtc cagaaaaaag tagtaccaaa acggcttcca
     2881 ccactgaaca aacaatggct acctcaaata atgagaaaaa gactgcttta gaccagttga
     2941 aagaacaaca gccaaatgtt cccatgcctc tagatgtacc agtctcttca ggctatctca
     3001 atatcgcagc gacacataca aagcaaggtt attctatttt atattacaga acagatcgtc
     3061 ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc tattgcaacc tatctatatc
     3121 aatatgggtt tgcttctagt caagaaacaa tccaagtttt gcagccattt gagatcgaca
     3181 caaatggtca gcaagtggac ttaggttctc gaatcacagg gtatcagcaa ggtgcagcag
     3241 gttcaagttt tcttgaatgg caagaaggaa actggcgtat tcgaatccgt gggaataata
     3301 tagaaggcca agatccctta ctattagcta aagaaatcgt agcttatttg gaagaaaaca
     3361 gtctaccagc acctgaacaa tttggtaaaa ttactgttga tatgggtgat acgacgaatc
     3421 gagccgtaga agtaagctgg caagaaccga aaaatgccta tacgatcact catcaagatc
     3481 ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt gtgaaatcag cgcgctgatc
     3541 tgagcccatc atttaactcc aagtcacagg aagtaatcaa aaatagcacg ccgcattttt
     3601 gcataatttt ggcttaatac cttaaaagaa aactagaatt gcctcttcac acatgctaaa
     3661 ataaaacaga caaaaaagaa atggggatat aagttgactg aatacttgaa tgtaggaaaa
     3721 atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta tctcaacaac ggatttccca
     3781 gaagaacgtt acaaaaaagg aacggtgcta acactattcc aagacggaaa agcacctgtg
     3841 gaattaacag tcaaaagtca tcgcaaacat aaaaattttg acttattgag ttttgaagga
     3901 catccttcta tcaatgatgt ggaaaaatat cgggatggca tattaaaagt gtcaaaagac
     3961 aactctgtag atctagctga aaatgaattc tattaccatg agatcatcgg tctgcatgta
     4021 ttagaagatg gaactgagtt aggtaaagta aaagaaatct tgtcaccagg tgccaatgat
     4081 gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc ttccatatat cgcttctgtc
     4141 gtcaaagaag tcgatttaga agctggagtg gtccgtgtag aaattccaga aggactgatt
     4201 gacgatgaag attgatgtat tgactttatt tccaagaatg tttgaaggtc ctatggggga
     4261 atccatcatc ggtaaggctg tagataaagg attattagat atcaatatct ctaacttccg
     4321 cgattattca gataacaaac atcagacagt agatgattat ccatacggcg gtggagcagg
     4381 tatgctgctc aaagtccagc ccatctatga taacatccaa gccattgaaa aagctgcacc
     4441 ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt aaacgattcg atcagaaaat
     4501 ggcagaagaa ttttcacaag aagaacactt agtattcatt tgtggtcatt atgagggata
     4561 tgatgaacgt atccgcacat tagtgacaga tgaagtttcc ttaggagatt atgtccttac
     4621 tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca gttcggcttt tgccagatgt
     4681 gttagggaat cagacttcag ctcaaacgga ttcttattca accggtttat tggaacatcc
     4741 ccaatatact cgcccggcag aatataaagg gatgaaagta ccagaagtat tgacaaatgg
     4801 aaatcataaa ttgattgaag aatggcaatt aaaagaatca ctaagaagga cctatcaacg
     4861 tcggcctgat cttttggaaa cacttgaatg gacacctcaa atgacgaaat ttttagaaga
     4921 aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc aagtgacttt ttctaaaatc
     4981 ttctttacag taggttgata atatgttagt attttatggt gagtgcaaaa gcacttaact
     5041 attacgatat tccgctgtga gaaaagctca taagaatatt tggaataagg agaagaaaac
     5101 catgaatcca ttaatcgaag aattaacaaa agaacaatta cgttctgaca tcccagcgtt
     5161 ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt gaaggtactc gtgaacgtat
     5221 ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct ggaatcagcg aaacttatac
     5281 agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca ttcccattgc atacaccacg
     5341 tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt cgtgcaaaat tgtactacct
     5401 acgtgcatta cacggaaaag ctgctcgtat caaagaaatc cgtcgttaat catcgttatc
     5461 agactgagaa agaacccttg gtattcaagg gttctttttt tgttctctgt ttttttatat
     5521 taacgatttc tatttgggac atttttgatt aataaaaagg ggcaaaaaag gggcaacaag
     5581 ttacagtttt tctatatctt ctttcaattt ttcttttact ccctttgtca catgcaaata
     5641 aatcttttca gtgatatcac tattttcgtg cccaacgcga tcctgaatag catacagagg
     5701 agttcctagt tccgctaatt ttgaaatatg ggtatgtcta aaaatatgtg aactaagttt
     5761 tttatcaatc ttcatgtcag ctttatgatt tctcaaatag gtgttaattg ctgttagttg
     5821 gaaaggagtt cctttcgttg tttgaaatag aaattgacca ttcggattta attctagaag
     5881 ctcattgtaa atagctatcg ctttttttgg taaatcaatt tctcgcattc cagcagcagt
     5941 ttttgtagaa tcagattttt tcatatcagc tattgaacgt tctctataca tcatcgtccc
     6001 atttataact actgatgcat tattattggt aatgtgtaca tcgtctttac ttaatgcgat
     6061 tgcttctcca ggcctcattc cagtcaaata tagccactgg aaaaggagag aatatctttt
     6121 attgtgtgaa gttgtgaaat ttactagtct tttatattca tcatcttcta aaaacttatc
     6181 tttaattttt atcgtttttg actctcgttt ataatcaata actacttcgt tgataggatt
     6241 tttttctaca tagccttttt tcatagcgta tgaaaaaaga agattcaatt tggatttaat
     6301 tacactgacg tacttatttg acaagtcatc tttatatatc atatcttcaa aaatattatt
     6361 taaatctact gtattaatac cagaaacgat gtaagtctca ggtatctttt tctttatagt
     6421 gtttaagata ttattcgtag gataatatgt agactcctta acttgtcttt tgtaaataac
     6481 taaccattct tcgacaagct catgaaatgt tagatcaggc ttctgaagtg ttttttcgtt
     6541 taattttata tcaatttttt tatttaactc tagcaatgcc actttctgag tttctcgaga
     6601 tttattttta taagtaatgc tcactttttt tctttttcta gtttttggat caatatatcg
     6661 ctcattatat ttatatactt tttttccgtt tttatcagtt ttggtttcaa tccacattgt
     6721 tttcatctcc tatttgttgc tataatagga atggataagt aagccaatta tagcaggttt
     6781 atttttcatc acgtccacaa actttggcga gggagggggc gtgttttttt gtttagtagc
     6841 tacctttcca tgaagtatct gcgaagaaca cttcaaatgt tgttccatca tttttcactt
     6901 caaaatatgc tgttccttgt gcggatttac ccggttggat ggtttccgaa aagtaatctc
     6961 tactagctaa atcagattta atatctgatg aatcataaaa ttctaaataa cttgaacttg
     7021 catcaaaagg tgtagtccct gtgttttcta caagaaaatc tacctttgcg taaaataggc
     7081 cttctggttt atagaaatca tcaccgtaat cttttgatac agactggaca gttacgttaa
     7141 tagagttacc attttcagaa gtaaaaggaa ctgcttctcc tatactatgt gcaactgatg
     7201 ccgtctgggg ttctgaagac gaagttgtag ttgtagttgt agtagagctt gaatctttgg
     7261 attttccatc tttatctaag aaagttcgtt gattataata tacaacctgt tcagtttttg
     7321 gactaaccca aattaacatt gtttcatcat cagaatcagt tgaataagta tattggagca
     7381 tctctaattt ttttatatct tttgcactat ttccatgttc aattaattca gagtgctctt
     7441 tataataatc tccttgtgca tcctttgaaa tagatacaat taattcatcc cgtgtgatag
     7501 cattgtctaa ttctttgtaa acaaaatcat ttgtccattt acgtaacggt gaaccaagtg
     7561 tctgataaac ttgagttaaa tcatcaccaa cttttatttt atgaacgtct gatgaagtta
     7621 gtttttttgt ttcatattca gatgttgatg aaacagtatt ttctttagtg ctagcagaat
     7681 taggactagt ggagcatccg cttaatacca agcctaaaaa aagcaaccca aaaacaattt
     7741 ttttcatttt taattcctca cttctatgat atgatttttt tgtaggatct tagaaataag
     7801 gtttttagtc cgtgttgcag cacggacttt ttttacatta tataagaaag cgataagcgc
     7861 tttctggaag tccgtaaaga ttctttaatt cctcaattcg tttaggatat tgatcattgt
     7921 cttctttgta tagagaaaca atgagattag cagcaaagca attagcttcg ctttcagatt
     7981 tgcttctaga tgttcttgtt gatacatagt aactggataa gccacgatga aaaatagcgt
     8041 gacctaattc gtgagcgcaa atgtagaatc tttcctcaga gtctctcagt tcatcattta
     8101 agaagattat tgcacgacct ctaatttctt gaaactgtcc tttgggattc tcgataaaag
     8161 gaacgtattg aattttaatg cccatctttt cacaaatata aaaaggatta gcggactggt
     8221 atttccgctt caactcctcg actaaattaa tcgtatccat ctccataagc tcacatcttt
     8281 ttgccttttt ctttgtcttc tttcacaata tcccagaagg ttccaataag gatatctttt
     8341 acgcgttgga cctgttcagg cgtcagtgtt tctccgccat aagacatatt gacatttgaa
     8401 tctagtaatt tatcaagttc aactacttca tcttttgttg cccagtcagg aacttggttt
     8461 ctaccgagta aataatcagt agttacatta aaataatctg ctacaagttg tagtctttta
     8521 gtactaggag ttctattttt ccattgataa atcgtgtttt taggtatatt taattcttct
     8581 tccaattgag ttatacttat gcctctttta tgagctaatt cctttattct atctagtaaa
     8641 ttcattttcg cacctcacaa gctacgaaaa cactaataaa aaaattagtt aaaggtgttg
     8701 acaactaata aaagtgttag tatactgttt tcgtaagcta aaatattagc taaaaagact
     8761 tgataactaa taaacacttc acggtcggca aactctgaaa tgtaaattac taggcatttc
     8821 tgtgtct
//
LOCUS       DARBFO010000102         8798 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000102 DARBFO010000000
VERSION     DARBFO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.105"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..776
                     /gene="menA"
                     /locus_tag="SCK51_002500"
     CDS             <1..776
                     /gene="menA"
                     /locus_tag="SCK51_002500"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEG1253075.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1590..2936)
                     /gene="gor"
                     /locus_tag="SCK51_002501"
     CDS             complement(1590..2936)
                     /gene="gor"
                     /locus_tag="SCK51_002501"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEG1253076.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3070..4323)
                     /locus_tag="SCK51_002502"
     CDS             complement(3070..4323)
                     /locus_tag="SCK51_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEG1253077.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4329..5252)
                     /locus_tag="SCK51_002503"
     CDS             complement(4329..5252)
                     /locus_tag="SCK51_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEG1253078.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5473..7965)
                     /locus_tag="SCK51_002504"
     CDS             complement(5473..7965)
                     /locus_tag="SCK51_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEG1253079.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7972..8439)
                     /locus_tag="SCK51_002505"
     CDS             complement(7972..8439)
                     /locus_tag="SCK51_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEG1253080.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8575..8648)
                     /locus_tag="SCK51_002506"
     tRNA            complement(8575..8648)
                     /locus_tag="SCK51_002506"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8612..8614),aa:Arg,seq:acg)
     gene            complement(8706..8779)
                     /locus_tag="SCK51_002507"
     tRNA            complement(8706..8779)
                     /locus_tag="SCK51_002507"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8743..8745),aa:Pro,seq:tgg)
BASE COUNT         2440 a         1867 c         1461 g         3030 t
ORIGIN      
        1 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
       61 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
      121 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
      181 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
      241 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
      301 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
      361 ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
      421 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
      481 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
      541 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
      601 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
      661 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
      721 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
      781 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
      841 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
      901 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
      961 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
     1021 cgtttatcag aggttgtaaa atcagtgttt tgtcctgagt attagagaaa caaatggaaa
     1081 aatagttcct catattttgc cgttttttga gctaataccg aaggacagct gattgaacac
     1141 tatttgttcg gaaaaagtga ctaactatga cacaattttt ttcacagtcc cttttttcac
     1201 ttgatttgat tcgtaccagc gaacttgacg agaactttct tggtttggta actatatggt
     1261 attcttttaa catcctcgtt tgcttcttct aatttattta tacaaagtca cactttttta
     1321 aacatctgtt cttcgataca tttctatttc ttttaaacat cgataccaat attttgaact
     1381 taagagtgca taccaaatgg attcatttac tcttcgtcag ttacgaagat aatcaaaatc
     1441 tccaacttcc ctacttattt cgttttgaga attatcgaag caatcttaca taaataacta
     1501 aaaaacaaaa aaccagaaaa tcagtatact gattttctgg ttttacatgg tttgttcaat
     1561 aaaaagctgt tggaactgtt aatattaaat tatcgcatcg ttacgaattc ttccgctcct
     1621 gttggatgga tggctactgt attgtcaaag tcttctttag ttgcacccat cttgatagcc
     1681 acggcaaaac cttgcagcat ctcatcgaca ccaacaccaa tagcatgtaa gccgacgatt
     1741 ctttcttctt ttccgacaca gatcaatttc atttcgcatt tttggcgata atcatttaat
     1801 gcaaagtaca ttggggtaaa gcgtgaacga taaactttca attcattttc gccatatttt
     1861 tccaatgctt cttcttctgt caaaccaatt gttgcaattg gtggatgcgt aaagacgact
     1921 gtcggaacaa gtttatagtc aagataagaa tctttctttc cattaaataa acgttctgat
     1981 aaacgtctac cagctgcgat tgcaacagga gtcagatcta atttaccgat cacatcacca
     2041 acagcaaaaa tatgttcatc cgttgtattt tggtatttat ctactttgat aaaccctttt
     2101 ttgtccaatt ccacatttgt attttccaga cctaattttt cggtattcgg tacacgtcca
     2161 ccggcaaaaa gtacacaatc accagttaag gtttcaccat tttcaaatag caccgtatat
     2221 tcgtcattgt ttttagtaat ttctttagca atatgatgtg cgtaagtttt tattccagct
     2281 tcttggtaca tttggatcaa attgtccgaa agcattttat caaacgttct aagtggtcgt
     2341 tcttttcgat atgcccacat gacttcacta cctaaaccac gaaatacacc tgaaagttca
     2401 gccgcaatgt atccagcacc taaaacgatt gctctcttag gtaactctgt taattcaaaa
     2461 aatccatttg aatcaattgc gtattctcca ccaggaatgg tcattttact tgcacggcct
     2521 ccagtagcga tcaagatttt aggagcacga taggtttcac cgtttacttc gatcgtttga
     2581 ctatcgataa aagtagcgta accttttata gcctcgacat gattgctatc taatcctctt
     2641 tgataagcac cgtgtagaaa atcaatatat ttttctcgtc tttctactag accggcgaaa
     2701 tcaacatttt ttaaatctgc ttggataccg taacttgagg catctcgttc aattgtttcc
     2761 aaaatcgtac ttgcttgcca cataactttt ttaggtacac aaccgacgtt gacacatgtt
     2821 ccgcccaatt cattcgcttc aatcagcaaa acttttgcgc catgcatccc tgctctgttt
     2881 gctgaagcaa taccgccaga gccgccacca attacgatgt aatcatatat ttgcattttg
     2941 ttcctcctta ttgaagagat caatcaaaac ctttatttaa cgatcctttt tctacagaaa
     3001 acaagtgtaa cacttttctt gaattttgta tgcgaagatg cttaaaacag ggatatatca
     3061 gctttttcct tatttacgtt ttcggatcat atctccaggt ttcaccggct gtgcgactgg
     3121 catagttaaa atcatcattg gatttggtgc tcggtcaatg gattcgcctt cttcattatg
     3181 cataacttca acggtttgac tgaaatgact gaatcctgga ccataaaatt cgatttcatc
     3241 tcctacagaa aacaagtttc tctgacgtat cgtagcaatc tgtgttgcct catcataaga
     3301 taatacttca ccgacaaatt tatatactgg gattttacga cgttcaccga ataattgttc
     3361 gttttcagtt ggtgtattgt agtaaaaacc tgtagctaat tctcgttgtg ctactttcca
     3421 taactcgtcg atccattctt gcttgcagac atagttttca ggatcttcca tataactgtc
     3481 tacagccgct ttatagacat ttgacaccgt agatacataa tggatcgatt tcattcttcc
     3541 ttcgatcttg aagctgtcta cgccgttttc aatcaattct ggaatatgtt cgatcattga
     3601 catatccact gcactcatcg agaattcttc ttccacttcg cctgtatcag ttaaactgtt
     3661 tcgttcttga ccaaaaggca tatcgaacaa tccatacttc cagcgacaag attgagaaca
     3721 tccgccacgg tttgcatccc gcatcgacat atggttggac aatgtgcatc gaccagaata
     3781 agagatacac atggcaccat ggataaatgc ttcgatttcg gtatccgtat ttttacgaat
     3841 ctctgccact tcctccattg aaacttcacg agctaaaacg acacgttcca acccttcttc
     3901 tttccagaat tccaatgtct caaaattagt tgctgaagct tgagtggata aatggatcgg
     3961 cagacctggg gcttctgtcg cacaaatttc gatcaaagct ggatcagaaa caatcactgc
     4021 agaaataccg acatcacgca aactgcggaa aaattcccct gctccatctt catttccttc
     4081 atgggtgacc atattagcag caacgtatac cttcgcatca tgttcttttg caaaagcaac
     4141 tccttcagcc atttcttcat aagtaaaatt acctgcacgg cttcttaaac catatgcatt
     4201 cccgccaata tatactgcat ctgctccata atggatcgcg gtttttaact tttccagtgt
     4261 tcctgctggc gctaggattt ctggtcgttt caattttctt tttactgtca tttctttcat
     4321 catactcctt atttgatagc agaaggatca atataaaaga atcctgtatc taattcacgg
     4381 ttttcaggat gcaatttact gatttcgcta gtcatttttt ccgctttttc ttgatcccag
     4441 ctaccttcta aaatttgttt ttttgcttcg gcgaatagtc gagtgatgtt tacgaatttt
     4501 tcgcctggag taaataatcc gtccaatttc catgtacagt aatgatgctg ataaagttca
     4561 ttcaattcat tcgagagatt cagatcattg ctggcaaaaa tatgtgtgcc atgactatct
     4621 tcataaatag aataatgtgt ctcttctttt ttaggttccg agataaaaag acctctttca
     4681 cgatcttttt gttcgtcttg tttcgtatag ttatagtaat tctgtagcag tggacgtttg
     4741 gattgatgga tgcatgtagc accatacaca agggtttcta caggaatatc gagtttttct
     4801 tccatcgcct tcatctcttc aaaaggaact tcacgagcaa gtactgctcc aactgcgcct
     4861 tttttcgccc agaaattgat ttgacgtgcg ctcgttacta atgtttcagc atcataaata
     4921 tacggtatac gcaattcagg attttttttc atcgtataaa taatacctgt atcaccaact
     4981 gtgatcacat ctacgtttat ttcttttaaa aaagccaaat attcaggtac atttttcatt
     5041 ttttcaggat gaatgatccc atttacagca atattcgctt ttttaccata cttgtgtgct
     5101 aattctacca attgtcgctg ttcttctcgt gtaaatgaag caggcagacg caatccaaat
     5161 tgctcttcac cgaaatatag tgtatctgca cctgcagcaa gcaactgttc tgcttgttca
     5221 atagtttcta ctgtggttat gagttcgatc ataattttct cctcctacaa cttcataata
     5281 gtagcatagc tctaaaaact ttccaatact tcttctattt aaagtcggat ttttgaaaat
     5341 tatttcacaa aaaatcaaaa taaagttcga tatattctgt ccaaatataa aaagagattg
     5401 tgacaaaaat cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca
     5461 caggccaagt aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt
     5521 gatcttacct tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag
     5581 caaagcctcg ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc
     5641 accatattca ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt
     5701 cacttgaata tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat
     5761 tgtatggatt tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa
     5821 gaattcaggt cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg
     5881 atctttagta acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc
     5941 tccgatattt gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga
     6001 atctgttaaa tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc
     6061 tttctccact tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa
     6121 ctgtccacct tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact
     6181 gtatttttcc ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat
     6241 gacttctgaa agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat
     6301 gaatgaacca attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg
     6361 tgaaactgct ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag
     6421 ttctaacaag cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc
     6481 cgatacaact gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt
     6541 tgcttcaatt gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa
     6601 ttgagcagca ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc
     6661 atcgatttct agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac
     6721 tttcgctgct gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat
     6781 ataacgaaca gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg
     6841 gtgctcttca tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg
     6901 ctcatctact tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt
     6961 ttgatattca tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg
     7021 tttcaagata ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg
     7081 caattcatca atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt
     7141 cattcgatct tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag
     7201 catcatcagg cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc
     7261 taacccttca gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt
     7321 ctttgtccgt cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac
     7381 tgggtccagc ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt
     7441 aggtgttcct tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat
     7501 tccttttcca gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt
     7561 tgaaaggctg atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa
     7621 taagctcatc aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct
     7681 gctagctaaa gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg
     7741 gtaagaacgg ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc
     7801 attcaaatcc atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc
     7861 tagtaaaaca tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc
     7921 aatttctaat actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc
     7981 atcttcataa cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc
     8041 atattcattg aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt
     8101 ttttgtaatt atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact
     8161 gattgtatca tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct
     8221 tacttttgca attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg
     8281 ttggatggta aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag
     8341 gtctgccatt tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat
     8401 atatgattca attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta
     8461 atattgacct taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa
     8521 ataaatcctt tagaatcaac aaaaaaaaat acggaatata ctattccgta tcgacgcgcc
     8581 caagaggagt cgaaccccta accttttgat ccgtagtcaa acactctatc cagttgagct
     8641 atgggcgcat attcaattta aaaaaattat aaatcatttg aaaacaaaaa gcaagataaa
     8701 aaaaatcggg aagacaggat tcgaacctgc gaccccttgg tcccaaacca agtgctctac
     8761 caagctgagc tacttcccgt ttaaaaaata aaaaagac
//
LOCUS       DARBFO010000103         8495 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000103 DARBFO010000000
VERSION     DARBFO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.106"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..174)
                     /locus_tag="SCK51_002508"
     CDS             complement(<1..174)
                     /locus_tag="SCK51_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1253081.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFLM"
     gene            complement(594..1715)
                     /locus_tag="SCK51_002509"
     CDS             complement(594..1715)
                     /locus_tag="SCK51_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEG1253082.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2043..3452
                     /gene="istA"
                     /locus_tag="SCK51_002510"
     CDS             2043..3452
                     /gene="istA"
                     /locus_tag="SCK51_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEG1253083.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3449..4177
                     /gene="istB"
                     /locus_tag="SCK51_002511"
     CDS             3449..4177
                     /gene="istB"
                     /locus_tag="SCK51_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEG1253084.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4305..5216)
                     /locus_tag="SCK51_002512"
     CDS             complement(4305..5216)
                     /locus_tag="SCK51_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1253085.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5233..6240)
                     /locus_tag="SCK51_002513"
     CDS             complement(5233..6240)
                     /locus_tag="SCK51_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEG1253086.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6237..8366)
                     /locus_tag="SCK51_002514"
     CDS             complement(6237..8366)
                     /locus_tag="SCK51_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1253087.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8371..>8495)
                     /locus_tag="SCK51_002515"
     CDS             complement(8371..>8495)
                     /locus_tag="SCK51_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1253088.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1729 c         1486 g         2681 t
ORIGIN      
        1 cattaaaaag ttagtaaata attgccctct aagttccacg gtctgttcat taataacttt
       61 atatcctcgt ttttcaaaaa atggctttgc ggtaatggaa gcgtgggtag tgattttttg
      121 cataccagtt tctaaaacat ggttttccaa ttcatctgca attttagtcg caataccttt
      181 tccttgatgg ctatgttttt gtcacaactt cagtattcct gtaacaatac ggatttcagt
      241 atacaacaac agcttttgaa aagatacttg caaaaggatt aagatatcta tgacctgcta
      301 tgctcactga tgttgaaaag aatgttttat taggattgag gtaagcaaga tagtgaatat
      361 gtccggaaag cctgtaaaat aaggcttttc ggacatatgt gaagttataa aaagatagcg
      421 aaaaatctat ttagtatcac taatgttgcg aacatatcgt cagaatagtc tgacatttat
      481 ttcttgctaa aaatttcaaa aaagtcaagt gctgacgcag cggatgacta ttttccagaa
      541 aattttagta tgtattcaaa aatcaaacgt gacaacagct attaactaca tcctcatcca
      601 ggtctgtaac gtttgaacca ctcttctgcg ggctcaaaca ttaagtaatg gaaggtacgt
      661 ttcaattgaa acagcgtcgc tgtcgtattc aactcaattt ttatcacata agtgagaagc
      721 gttgcgatta aggtgaggat gacttgattt atcgcccctt gttcactatg tgaatatagt
      781 cgtttaatgg tcagattttg ttttaagtgt ttgaacaaca attcaatctg ccaacgggct
      841 tgatacatgt cggccacttc gtttggcgat acatcaaaga gattggtcac cattcgcagg
      901 attttttgtc ccttggtaag aaccgttacc aacctaaaac gctctgtcac atggttgaca
      961 ccgccgacaa gaatcacttg ttggtcactg attatcgtcc catcacgggt tgttgtatct
     1021 gctacttcaa tttgatccaa gatggtaatt tttgtattag attttgtccg tgtaacgaag
     1081 aaatagccgt cacgatttag tttgtctaac aacttgtaat caaagtagcc gcgatccacc
     1141 acatacgttg cttgcgtttt atcaacaaga acttcaaggt gattacggtc atgctcactc
     1201 gcatttgtca tcgtaaaact ttcaggatac tgattggttt tatcaagata acagagattc
     1261 aaatgtagct tgatgccaga ctttgttttc cgaaacttgg cccatggaaa ccatgctttg
     1321 ttcaacggaa tagtcgttga atcaatcaat tgtagactgg ttgtttttga gcttggctgc
     1381 tgagcagaaa ttctggcgac taattgcgta aaaatttcca tcaatacttc ttgcttgatt
     1441 tctggcgctc tacgagataa cgagctgtaa cacagggaat caatgccaag ttccttgcag
     1501 aggcgctgat ctaaaaaggc gcgatcaatc tcgcgatagc ttggcaattc ttcataaacc
     1561 gcatgaagta acactttaag cgtcgtttgg aagtctagtt ttttactgta gaaatcaaag
     1621 tcgctaatta agctttgaga gttttctgat aaaccagaaa aatttattgc agaaaaccat
     1681 ttttgaaatg aggtttttac gttatactta tccatgcgtg agtccttttt attggtcttg
     1741 gataagtcat ccgagaatag tataaaggac ttttttgcgt ttggatagct ttgtattagg
     1801 cttttgatgg gttaaaaagt ctttgcaaca ctagtaattt agtatttatg tataaattga
     1861 aagatatttt accaccactt agtcttctta aaagtgatcc aacattttgt ttgtggcaga
     1921 atttaactac acactaatgc agccgaaagt acacaactat gcagcagttt gactgcgaac
     1981 tggtttcctt ccatcataag tttgaatgac ttagtgatgg aggtacagaa aggttgttaa
     2041 ctttggatga tattaaaaca ataaaacatt tgagaaataa tcgtgatcag tcaattgatc
     2101 agataagaaa agcaatgaat atcaattggc gaacagcgaa aaaatacgcc gatagtgatc
     2161 aactattcat tacagatccc actcgaaaaa acaaatccat gatggatgat ggttggggag
     2221 aaatcatcgg tagttggcta gaagaagacc tacgtttacg cagaaaactt cgtagaacgg
     2281 ccaaagctat ccatgaacaa ttgaaacctt tgggtttccc tggctcctat cgaacagttt
     2341 gtgtatacat ttctcaatgg aaaaagcagc gttacgacga aacgaattac caagctgtcg
     2401 atcgtcttga tcatccgatc ggagaagcac aggtcgattt tggaacaatg gaagttatgc
     2461 atgaagatca catgaaagat atacaacttc ttatcatgtc atttccctat agtaatgcga
     2521 cgtccgcaag tgccatgcca gctcaaaacc aagagtgttt cctggaagca ttaaaacaac
     2581 tatttaatca atgcggcggc gttccaaaaa cgatcagaat agataactta actccagcgg
     2641 tgaaaaaaac aaaatcaaaa tttgaagaag cacaactaac cgatgaattc ttacgtttca
     2701 ccattcatta tggttgtgat gttcaagtct gtaacccacg tagtgggcat gaaaaaggac
     2761 atgtcgaaaa taaagtgggt tatgtccgat acaatttctt ctcgacttcg ccaatcatgg
     2821 actcttttga gtccctaaat aagcaacttg aagaacaatt aaaagcagat cgaaaaagga
     2881 cgcattattc aaaacatcaa ttaatcgaag atttgtggaa gaaagaaaat aaagcgttac
     2941 attctcttcc acaagaggat tacccaattt ttaaagaagt ggaagctaaa ctaaatcgtt
     3001 atcacgaaat gaaattggat caaacggtta ttcatattcc taaagcatat ggctattctc
     3061 aattgactgt tgttttgact tggaataaat tcaaagttct tacgcctcat ggagaaatat
     3121 taatgaacga atatcgcccc tatatgaaca aaaaaagatt gatcccatgg agtgacatca
     3181 tgaaacaatg gagtaataaa ttgaatgtcg ttagttattc gaggtattgg aagtacttgc
     3241 ctgcacgcct tcaaaattat ctagcgattg ataataatca cctgaaatac agacggataa
     3301 aacaattact aagtttatta gcgacctacg atatccaaca aattaatgaa caattttacg
     3361 aattagtcgt tcaagaagac gccccctctg actttgatgt gaattggtca aactatgatg
     3421 cattaacaaa aacaggaggt gtgcaaaaat gattggacag acgttagaag attattgtaa
     3481 aaagcttcga ttaagccata tcatcacgaa cataccaaga gcttctttcg aagctccaga
     3541 acaatttatt accgatttat tccgtttaga actggagacc agacagcaga tcaaattaga
     3601 acggatggaa aaattagcca aatttcctgt accaaaatcg ctaaagaact atgaatggca
     3661 cgaagatatt tttctaccat ctcatctaga taaggagacc cttgtctctc ttgattttct
     3721 aaaaaggcaa gaaaacattg tttgtgttgg ctctcctggt acagggaaaa cacatctagc
     3781 gatcgcatta gggagaaaag cctgtagtga aggtgtttca accaaatttt tccgagttaa
     3841 caatctcgta gagcaattag agaatgcatt aaaaaaacaa cagctagcca gtttcaagaa
     3901 gcgattcgaa acaacagaat taattattct tgatgaaatg gggtacttac cattgacaaa
     3961 agaaggagct gaacttctct tccaactgat ttcagattgg tatgagcaaa agagcattat
     4021 tattacctct aatttagaat tcagtcaatg gaatcgagtg tttatggatt ctcgattaac
     4081 agctgctctc gttgatcggc taatccatca cgctcacatt atcagcttca aaggcgaaag
     4141 tttcagattg aaaaattcat tatctaaatc atattgaagg aaaaaactag ttgcagcaaa
     4201 actctgcact ttcggctgca tttttttgta aaaagctatt gcacaacaca aacatttgaa
     4261 cgacgtaatt ccaaatgttg gattgctttt agataatctt ttatttattc acaattgcta
     4321 atagtccaat tttcattttt gtatagtttt aatctatact gaaaaacact tgttgttttg
     4381 gacaactcat caagatactc aacaccaaca atcacttgaa tcgctttacc cttttgtttg
     4441 taaataggat tcactaattc cacaaatttc aaattagtat taattggttg catcgcgtct
     4501 ttttcaacat aatgctctag ttctttttct gaggctgctg gataaagcct gaaaaacgtc
     4561 tctaaaaact cggtgatttc ttccgttgtc tttgaatcaa tttttgaatc actttttact
     4621 tgtcttactt tatgttttgc cttttctggc ttaccaatca tagtgggtaa actcgttaca
     4681 atatgattgc ctttttcatc ttcaaagatc atcagacgat atgcagaaga aattgattgt
     4741 gtctttttcc cttctgtaat cttttgatct accacaaatg aaacgacaaa ttcttccgag
     4801 tgtttttcaa catctagaat ttttactgat acaacctcgg aactagtggg aatatccgct
     4861 cgaaccatat cttggctcaa agcgagccct tcttctgtaa ggtactgctc aagattagtc
     4921 attctctttt caattacctc cttgttattc ttccatgaaa aatattcttt cacaaaatct
     4981 acagtaaaat tttctactcc agaggtattc actattttct ctttcacgac cactttctca
     5041 tgagttgtat gctgatcaat ggccgtgaag tttttgtaga ttccaaaagc aaggctacaa
     5101 gacaataaaa gccaaagggc aagcaccatt tttcgatgct tgcccacgga tactactcgt
     5161 tctttcttct tttttggaat cttttccttg ttacttcgct caatttttat cttcattttc
     5221 cattcctctt ttctattgtt tataccgacc agcaccaata atgtgctgtt gccaatagct
     5281 atcggtaaga ttggtccaac ccaccggatc tcctgcatga aacattctgt tctctcctgc
     5341 ataaatgccc acatgagtca cataagtacc ggcatcataa gttgaatgaa agaaaatcaa
     5401 atcgcctggt ttggcatctt ttaagtcaat atgagtcata gcatcatatt gatcctgagc
     5461 cacccgaggg agtttcaaac caactttggc ataacaccat tgagttaaac cactacaatc
     5521 aaacccagtt ccgggacttg acccaccaaa tacataagcc gttccttcat acttgaacgc
     5581 ctcgtcaaaa attccttgga ctgttttatc atcaaactgt gttgtagtta ggtactgctt
     5641 cactaataag caataaaaca tattgccata gttataccgc cacccactat tttccttaat
     5701 cgcgatggga ttgggataat cgactttttg accacccgat ttttcttttg aaaaagattc
     5761 ggctaaagca aaactatgct tctttccgtt tttcgcgaca taatccaaat acccgtcacc
     5821 atagttataa gcctgaatga tactatcttg atcgcatcct agttctttgg aacacttaac
     5881 caaagacgaa aaatagagac acacttgttt gatggatgcc tcggtactta atgaattcgg
     5941 tgataaacct gctgattcag aactttgcat gacatcttcc attgtacctc cagattcgac
     6001 ttgaataatc gccaaaagaa tcgaaacata ctctgttata tctgattctt tggcatactt
     6061 ttccaccatt gaacggtgtt tcaaaactac ttctgagaca ctactacctc caggagaagt
     6121 tccagaacta ccacttccct caccgtcctc accaaacaac aaacccacac acatcaagat
     6181 gcccacaaaa gagatggtca ggaaggttaa cgaaataata ctcaattttt ttaagttcat
     6241 cttttctcac ctggctttgg ttttgggcga gtgttctttt ttctttgtac ttgttgattg
     6301 gttcgctttg gaaaagtgaa tctctgtttc acttgttgaa aggaattgtt cttgcttagt
     6361 ggctgagtgc ttccgtttgg aatgacttct tgactagaaa gtttccgttt gatttcagga
     6421 ttcgaatgta cttgtaccct tggttttaaa gacctatttt ctggtagttc atttttctgt
     6481 ggttttatct ccttttttat cggaacattt tcccttcctt tctcatgacg attccatgct
     6541 tcgttcaata ctttgcgttt ttcatccatt tttgcgtctc ttcgttgtcg ttgtttttct
     6601 cgcaactccc ctcgatttgc tttttcttcg aaaggccctc gcttaaagtc ctctggtact
     6661 tttttcgtct tttccatgcc tttatgaagg ctgtatttca agttagttgg tgcatcggtt
     6721 acttgatttt ttgcttgttt cgctttgtcc ttcacgattt cttttgtatc gagtactttt
     6781 ccaacttttc taccaactaa atttattcgt gattctttct tcgtgcccac tggttgttct
     6841 tgttttttca gatcagaatt tagctcgtta atgcttctcg cggaatggtt ctttctattg
     6901 gacactctat tgatttttga actacctttc ccttttgtag aactggtagc tagtttccca
     6961 ccaactgctg caccgccacc aaaagcaaaa gttcgaccca catttcgatt aagctgtcgc
     7021 aattttcgat tcatcaacat ttgcggtttc cgcatgactc gccttcccat tgactgacta
     7081 tcattacttt gaaggttaaa catgctcata atgtcaccta atttgaaata gataccggca
     7141 aaggtcacaa tttgtagaaa agctaccata aagaagggat aggtggccga aatattgaaa
     7201 aacatcgtgg agatactgaa tgcagtcgta atgactaagg taactcccgc tcgcatcata
     7261 atcgtattga acaaacgaat aatcgccctt ttgcccaaac tctcatacgt cggcaacatc
     7321 gacaataaga aactaatggg cagaaacatc gcaaagataa tgaaaaggat ttgtgagaac
     7381 aacataatcc ctgtcagaag aaacacaaaa aaggaaatcc caatattaaa caacactaaa
     7441 aagaccacgg ttcctaaacg gttcatggtc ttggtaatac tgagattcgc attgtcatta
     7501 tcttcaatct ctgctttgac tgcttcttcc cgatccttac ccttattttc atccggactc
     7561 acggataaaa tcttattaac ccgaccctcg ccaatttctt ctatattgga atcatcgaat
     7621 tgtaatagta accagggttg ttgaacttga atcgaaaata aactgtctcg aatcaaatcc
     7681 acactatctt ttccctggct ttctgaatta ggtacaacaa tcttggttcc aaggctaaga
     7741 gccgcttcac taacgtctga gctaaagtca ttgatttttg taatataggt gggcgcatac
     7801 gcaatgaaag aacccgagag tagaaaaatc atcacaaaat taatgacggt tcggactgct
     7861 ttggtagttt ctcgttttaa taatccggta tacgccacat aaactcctag agctaaaatc
     7921 atcaataata ggaacccaaa gtagaatccc gaagtctgta atccattttc agtgataccg
     7981 gctaaagttt ggatattctt tccaatactt ttagccgtat ccgaaatgaa atccaacttg
     8041 taagcttctt gaaccacata gcccgtggcg tttgataaat acagacttac tgtccaaata
     8101 aaattggtaa tggcataaag accatacatc acgcttttcc ctaagccatc cccccaattc
     8161 caaggtaacc aatcccaaga actatccaca aagaaatcca gttgataatt gttcagagaa
     8221 tatttggaat aaaggtttcc ggcttcaact gtatcatcaa ccagacctgt cgcctcagct
     8281 accgtaccga ctaaactcaa tagaactgtc agagtgacga tgctaataaa aacgattccc
     8341 acaatccgaa aaagtttctt tttcatcccc tcactccatt ctctcttgaa ccggcggacg
     8401 agtgtcaaaa gcatggaaga gttcttcaaa gactggatga acttgaacca cacctactcg
     8461 cccatacaag tcttgcatca agcattgacc gtttt
//
LOCUS       DARBFO010000104         8348 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000104 DARBFO010000000
VERSION     DARBFO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.107"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            205..684
                     /locus_tag="SCK51_002516"
     CDS             205..684
                     /locus_tag="SCK51_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253089.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            717..2945
                     /locus_tag="SCK51_002517"
     CDS             717..2945
                     /locus_tag="SCK51_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1253090.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3074..4717
                     /locus_tag="SCK51_002518"
     CDS             3074..4717
                     /locus_tag="SCK51_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEG1253091.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4790..5335)
                     /locus_tag="SCK51_002519"
     CDS             complement(4790..5335)
                     /locus_tag="SCK51_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEG1253092.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5358..5843)
                     /locus_tag="SCK51_002520"
     CDS             complement(5358..5843)
                     /locus_tag="SCK51_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1253093.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5833..6645)
                     /locus_tag="SCK51_002521"
     CDS             complement(5833..6645)
                     /locus_tag="SCK51_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEG1253094.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6784..8316)
                     /locus_tag="SCK51_002522"
     CDS             complement(6784..8316)
                     /locus_tag="SCK51_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEG1253095.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
BASE COUNT         2815 a         1589 c         1643 g         2301 t
ORIGIN      
        1 aaatattcca acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga
       61 gatggtgttt ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct
      121 ctataatgtc gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa
      181 ataaaaggat gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta
      241 atcgttgtag aagactatga taaagttgat ggaagaaatg cattaaatat ggatgcaaaa
      301 gggctttcac tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag
      361 gtatggcgac atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt
      421 attttagatt tagcgatctt agctgcgcaa ggaagtttat acttccaaga tgcttatcgt
      481 tatgaaaaat tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac
      541 cgaatgacgg tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat
      601 tacgatacac tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt
      661 cttttagatg agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg
      721 agaatttctc actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga
      781 agaaaccgaa aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag
      841 ggaattggca agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt
      901 cggggaagac acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag
      961 tgacctgttt tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag
     1021 attctaataa ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg
     1081 aagctgtact gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa
     1141 aattagcaga gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct
     1201 attacccttc tgaaatcgtt cgagcgattg aaaaaggcta tttattagag attcaagagc
     1261 cgactgtcat ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg
     1321 ggatgttgaa tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca
     1381 ctacaaatcg aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc
     1441 aacatgcgga aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga
     1501 aaactatgat ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg
     1561 atgaaactgc aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt
     1621 gggcaaatac ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt
     1681 ataaattatc cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg
     1741 gattgctact ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg
     1801 agcatacaaa agaaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa
     1861 atgctgaaga aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg
     1921 agggagaaga gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa
     1981 aagaacgcag tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt
     2041 ctcaagaaat gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga
     2101 ataaagaagc gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc
     2161 accgaccagc tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc
     2221 caatagtaga atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga
     2281 cgtaccaaaa aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg
     2341 atttgagaaa ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg
     2401 tacggatcga tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag
     2461 cagcgattgc cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag
     2521 atacagcaga ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag
     2581 acggatttca atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg
     2641 atggtgctgc acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac
     2701 tgttgcttaa tattagtgac ggtcagccaa aagcattgcc ggattataca ggcaaaaaag
     2761 caaaagaaga tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct
     2821 ccgcagctat tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg
     2881 tagatattac tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc
     2941 tatgataaaa taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag
     3001 ttaccggtaa caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa
     3061 ggagtgaaac gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat
     3121 ctatcctgct agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg
     3181 tgaaaaactt aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta
     3241 tcaatctcca tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag
     3301 attcggaacg atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa
     3361 agtgattatg gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc
     3421 gaaaaaatct aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg
     3481 caaagaaccg aataattgga catcgatttt cggcggttct gcttgggagt attctcatga
     3541 aactggacag tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag
     3601 cgagaaattg aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga
     3661 tggatttcgt ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac
     3721 agagaaccct ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt
     3781 gaaacatgtt tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc
     3841 agaagaagga ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga
     3901 tcatatccat atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaaca
     3961 tgcattaagc gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga
     4021 aaaccatgat gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc
     4081 tatgtctgca aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta
     4141 tcaaggacag gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc
     4201 agtagattcg aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc
     4261 attggatatc attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc
     4321 ggaacaatat gctggttttt ctacacatga accttggctg atcactaatc caaataccaa
     4381 aacgatcaat gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa
     4441 catgatccgc attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct
     4501 tcatgaacat ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat
     4561 tatggtaaac ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc
     4621 ctggacatta ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc
     4681 accttatgaa gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta
     4741 gttggacttt gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg
     4801 ctctaggacc accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa
     4861 gggttttcaa acgaggtctg acaggtactt ggacatgctt gatatgcata ccgattgatg
     4921 tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga
     4981 actggtcaaa agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga
     5041 caatttcgaa attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat
     5101 gttcacaacc ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac
     5161 ggatgatcca ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt
     5221 cgcttgtgct gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata
     5281 tgtcattaac tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct
     5341 aaaaccttct ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt
     5401 ataggaaagg caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc
     5461 actccagccg gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact
     5521 aagcttccta ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca
     5581 gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct
     5641 gaaatcaaat cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca
     5701 taaagaaaag cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc
     5761 ggtacaggga tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag
     5821 atcgttaatt gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac
     5881 acttccggta tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt
     5941 actttctgct actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga
     6001 agaaaaggca caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac
     6061 ctcataatga tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc
     6121 gcctttgatc acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat
     6181 ttctgttaac gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg
     6241 cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt
     6301 gatcacagct tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg
     6361 ttttgttttt atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat
     6421 tttgatacca tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc
     6481 caaagatata gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa
     6541 ggcgcttagt ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc
     6601 accagcaaat ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat
     6661 gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct
     6721 catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa
     6781 tagttaggac ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt
     6841 agctgttcgc gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc
     6901 atggacttgc tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat
     6961 cggttgtgga gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga
     7021 atctaataaa aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg
     7081 ccgatctttc aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca
     7141 ccaatcgcca taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg
     7201 tcctttagac agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga
     7261 atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact
     7321 tgttttcaaa ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc
     7381 atatacttga agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt
     7441 ctggttgttt atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg
     7501 atgctcctta gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat
     7561 tggccggtga gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat
     7621 cataggaaat cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg
     7681 agagatgtga aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca
     7741 tttcgtccgc catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt
     7801 accagaagta tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga
     7861 caaagcaggt tgttccatta aataaagaat aggaatctgt tttgattttg ttccatttga
     7921 aatttctctc actcgctttt ttgtctcatc atcaaataaa atgaaagggt cttctaaacg
     7981 atcgataaat acttccattg ttgttgttgg tagatgataa gaagtcatga caggcactgc
     8041 accaaggtaa gacacagaaa cagctagaag gtatgtgtca aaagcggaac tcttataaat
     8101 aataactttg tctccggatt tcactccaag atgagccaat tgataagccc gtttcaacac
     8161 actttcgcag cattttttgt aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg
     8221 ctcatcaaag ataatcgggg tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa
     8281 taagttcaaa ggggaatacg ttatggtctc catcattcac actccattat cttttgttaa
     8341 gtaatata
//
LOCUS       DARBFO010000105         8200 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000105 DARBFO010000000
VERSION     DARBFO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.108"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..866)
                     /locus_tag="SCK51_002523"
     CDS             complement(<1..866)
                     /locus_tag="SCK51_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HEG1253096.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDE"
     gene            complement(872..1978)
                     /locus_tag="SCK51_002524"
     CDS             complement(872..1978)
                     /locus_tag="SCK51_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEG1253097.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            complement(1978..3114)
                     /locus_tag="SCK51_002525"
     CDS             complement(1978..3114)
                     /locus_tag="SCK51_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG1253098.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(3146..4597)
                     /locus_tag="SCK51_002526"
     CDS             complement(3146..4597)
                     /locus_tag="SCK51_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEG1253099.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(4641..5405)
                     /locus_tag="SCK51_002527"
     CDS             complement(4641..5405)
                     /locus_tag="SCK51_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEG1253100.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(5433..6131)
                     /locus_tag="SCK51_002528"
     CDS             complement(5433..6131)
                     /locus_tag="SCK51_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEG1253101.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(6143..6922)
                     /locus_tag="SCK51_002529"
     CDS             complement(6143..6922)
                     /locus_tag="SCK51_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEG1253102.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(6938..7882)
                     /locus_tag="SCK51_002530"
     CDS             complement(6938..7882)
                     /locus_tag="SCK51_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG1253103.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7928..>8200)
                     /locus_tag="SCK51_002531"
     CDS             complement(7928..>8200)
                     /locus_tag="SCK51_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253104.1"
                     /translation="NMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQV
                     TATATYDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2324 a         1728 c         1207 g         2941 t
ORIGIN      
        1 aattcgtcac ataatggatt gttactaggt attttatcaa atgtctgtgc tatttcatga
       61 tttattctct gtgctaaaac aatcatataa tctatcatca aataatccac catcatatca
      121 ttatttttcc aataatttaa aaaaaagtct ctaattatag caaacatcca tctagactct
      181 tgattacatg ctaatgaata acccgcaaaa tatcctgatg ctacggatgc gtgagcataa
      241 tcaggcctcc taatagacca aatatcgata tcaaaatact catctggtat tggttttgta
      301 caataaaatg tggcgtctaa ccacaaacct ccatgttttg ctagcaaaga taatcttaat
      361 aaatcagaat aatttgttct cgtaatgatt cctttgtttt tcttttcttc cacccatttt
      421 ggtatatcaa catattgtct ataattatct tctgttaaaa ttataacagt atgtttccct
      481 gcatttcgtt ttattgattt tacacaagct tttacaattt ccggcgattg atccaatccc
      541 tgccaccaac aaacccaaat gcagttagaa tatggtgatt ttgattgatt aattatttga
      601 ttttgactat aatcatatgt tttcaaaaaa tctgaaaaag tcttttcata atacttcagc
      661 attacctcat gtttttttaa taaatgttcc ttctgttttt tactttcatt atatccatta
      721 cgattcatga cttgaatatc aattttagct tgtaacgtgg caaatgcttc cttccaagat
      781 gttatctttg caattcgtct tgttgcattc aattcttcgg atatgcgatg tataattctt
      841 tttatacttg aatgtttctg tgtcatattc tttaacctct ttgtattaat tcaaataact
      901 cataatattt tgattctaag attttgtatg ctgtttgaat attataatct gcctttatta
      961 aaatcgaacg ttctgaatct cgatgagaga tatctacatt tgcaatctct ctcgcccatt
     1021 cttttgccga ttgatttaaa cttatgaaat tggtcttatc actgaaagca acttctgtag
     1081 gaactttctc cgaaaagaaa cacggtaaat ctgcaaattg agcttcaata cccactaacg
     1141 gaaccccttc aaataatgat ggcaacatta atacgtcaaa tgcttgataa aatttttcta
     1201 tatcatttct atttcctaaa aatttaatat catcatctat gccacttttc tttgcataat
     1261 tttttatttc ttcttccata tcaccagttc caataattaa taatacggaa ttattcttaa
     1321 tagcgtgaaa ttctttaaaa atttcaatca aaaaacgatg atttttagaa tatgaaattc
     1381 ttccaatatg tcccattaca aacgtctgac ttgaaattcc taatttcatt cgtacatcat
     1441 ttctaatact ttcatcataa taaaattttt tagcatccac tgcatttgga atcaaagtga
     1501 atttatcatt tcgaaacaaa aaatcacctg ctacttttcc acatgaaaat tcatgtgtca
     1561 aaacagacgt taacttacat cgataatatt gctttaaaag atatttaaaa tctctatcta
     1621 tcgatgtatt atggctatgt gctatccgta tcggaatccc agcttttttt gcagctttta
     1681 aaggtaaata actcatcgca tcaatatgag aatgaacaat ctcatattct ggatgatcct
     1741 taaaaaattg ttccatccat ttaaaatatt taggataatt ttgtggataa agtctcggtg
     1801 cataatagac ctttcctcca agacttatta tttcctcatc ataatcactt ttatatggac
     1861 gatgtgtcaa aaaatcaaac tgaattttag ttcgatctat attcctataa tagttcatta
     1921 gcatcgtctc caaccctgca cgatgcatat cattcacaca ctgtaatatt cgaatcatta
     1981 tatatttccc ctatacaaat ttaatgtttc ttgtacgatt ttatcccaat tatatttact
     2041 acaaataaac tcagtagctt gattcttcaa aactttaacc atttccgatt gattgcatgc
     2101 ctcttctaat cttatcttta aatcacttac atccgatttc ttaaaaatca tagccttgtc
     2161 ttctactact tccgtacact ctgagatatt tgaaactata caacagttcc cataactcat
     2221 tgcttctaac aaacttaatg gcattccttc taaatcactt ggaagtgtat agatatacgc
     2281 attactatat aattcttcta attcctgtcc ttgtacaaat ccagtaaata taattcgctc
     2341 atcacctttt gctaattcct ttagttcatt tgcaaattca tctgtatcag agcttcctcc
     2401 ggctattata agttttttat ctgtctgaac atctttaaat gcctcaatta gataccgaat
     2461 tcctttttcg ggaaccaatc gccccaaaaa caaaatataa gaatcttttg ttaatccata
     2521 cttttctgta atcaatcctg ctgattttat ttcaattttt ttaacgccat ttggtataaa
     2581 ccttgttttt cttccatata tatcttcaaa ataattttgg acgctttcac taagtacaat
     2641 tatttcatct gcaaatttta cagcacactt ttcccctaac atgatgtatg tactagcaag
     2701 cttgttccat ttttctcttt gatgatctag accatgaatt gttgcaacac atcttttccc
     2761 aaaaagtttt ggcaaccaca acatagcaca tggtccttct gcatgaaaat gaacaacatc
     2821 atattttccg aatgctgctc taattccacc aaatactgaa gaactcattg ctgcaattcc
     2881 ttttatattt aatgtaaaaa tagtctttaa ttttacacct ttatattctt tatttttatg
     2941 ctggtcaaat tcctttccgc taacatgatg accacttcga ttatagcaag tcacttcatg
     3001 cccaagtgca accattcgcg ttgccaattc ttctaccact atctcaattc caccttctct
     3061 tgatggaatt cttttatgac ctagcataca gatttttaat ttttttcttg ccatcacgtt
     3121 ttactccttc tatagtatct ttatcttaca tggaaccgtt tctatgaagc acagttttga
     3181 ctgtcctaaa caaaattcta aaatctaatc ccatactcca attcctgata tattctgcat
     3241 caagttttac aacctcttcg aagtctgtga tatcacttct accactcacc tgccacattc
     3301 cagtaatccc aggtttcatc gcaattctcg cacgatgatg aaaatcatac ttctcatatt
     3361 ctgacttcaa gcaaggtcta aaccccacca ggctcatttt tcccattagg acattataaa
     3421 attgtggaaa ctcatccaat gatgtagatc gaataaattg tccaattcct gtcttctttg
     3481 ttccatcagg aagtatttta ttgccaatga ttcttgggtc aaaatccatc ttaaacatga
     3541 gattattact catcttattt tctttcatca actctgcttt gcgttcttct gcatccatat
     3601 acatactacg gaacttatac atcttaaatg tcttaccatt ttgaccaata cgttcttgtg
     3661 caaagaaaat cggccctggt gatttgatgt aaataactgg tgctacgaag ataaaaataa
     3721 cacctgttgc aatacatcca ataattccag caagaatgtc aatcgttctc tttataaatg
     3781 cttgcctcat cgtcataaca ttaatactag atgtaataac agtataatca ccaactcggc
     3841 caataaattg ttttccacgt gtactatcag acacttttgt aagattcata tgtacagcaa
     3901 gtcccatttc cgaacatctg gcgattaatt ctttgggata tggaacactt tcgtcaacat
     3961 tgatgaatac ttcatctacc caattctgta aaagatattc cgctgcatta tcagcatttg
     4021 caactactgg gacacctccg atggtagttc catacatatc tctgtctact acaatcacac
     4081 cactaagttt atacatgcca taattcttct ctttaagatt acgaactaca ttttttgcaa
     4141 tcttttgaga tgttacaata actaacgata catcttcacc gtttttcatg cggtgatgca
     4201 acaacccttt ccaaagtata cgaattatat atgttaataa tccatacacc acccccatta
     4261 taaataaaga aactctggaa taatcatttc catcctgcat tgtaataagg tataaactgc
     4321 acacaagtaa cagcataaaa gattgcttta tagaagctac agcctcttta taatatcctc
     4381 tttttaaaac atttttatat gcctcaaata aaaatataat cgtaagattc tcaagttcta
     4441 taaaaattgc catgtggcga taaataggaa tcacatacgg attccagtca ccctgtcgca
     4501 ttacatatga tatataaaaa gcaagctgga cacaaaataa gtctaataaa ataaaatcaa
     4561 tatgtttaga ccaaccactt gagttttttc tatacatctc tcactctctc ctcatttcct
     4621 ttttatctac gaataaagaa ctaaaataaa ccaaacttct tcttttttac ctcttcatag
     4681 attggataat tgatctcatc cccgttgatc agatcctttg cgacttgttt catctgtact
     4741 actttttcct taccaaactc cttggcaatc tgctcatagc actcttttaa gtaaaaggtg
     4801 cgcttagaga ccccgtgtgc atcagaagca gccatctgca cgagattatg tttgaccatt
     4861 gttttggctg ttttctggat atctttgcca aatacaccaa cataacttgg cgcagtaagt
     4921 tgggccaaac atcccatatc taggaacgga agcaaatgat ttgggtcttc tcgaaaatgc
     4981 gcgttgcgtt ctggatggac gatcactggt atctttccta gttctaacaa ctcaaaaaaa
     5041 agccgttctg catataacgg cacttccagt gtcggaaatt cgatcagaag atatgtatca
     5101 tctaaatctg taaataaaat ttcatctcgg tggatgtctt cgatgagcgt tccggtaata
     5161 cgtacttctt gtccttccaa taacgtcaaa ggcaactgtc ttttctctaa ttcggcttgt
     5221 aacgaagcga ctagagaaat gacttgtgat ttttcattgc tgtattttcc attattgtgg
     5281 tgtggtgtgc ataaaatatg cgtaatgcct tgctgaatcg ctttttcagc cattgcaata
     5341 cttgcttcta agttttctgc cccgtcatcg attcctggta ggatatggca atgtagatcg
     5401 atcatttctc ttgtctcctc tcttcttatt ccttaatttc cgtagtaata accagcatct
     5461 ttgctgtgtt ctgctccgtt gtatacgaca cctaagactc gtgcttgtac catatctaac
     5521 agttcttttg ctttagtcaa tgattctttt cgtgacgtat tttcccgtac gaccaataag
     5581 gtgccatccg tttttgaaga catgatctgt gcatctgtta cggcaacgac tggcggcatg
     5641 tcgaagatca ctacatcaaa tagttgtctt gcttctgcta aaatctgatc cattcttggc
     5701 gaacttagta gttctgatgg atttggtggt ttaggtccac ttggcagaat cgacaaattg
     5761 tcgactggtg ttcgttggat ctcatctgct acacttcctg atgaactaag aacagtactt
     5821 aatccgcttg cgttgttcaa cttgaacgtt ttatagatga cgggcttacg taagtctgca
     5881 tcgactagca gcacccgttg accagatttg gcaaaaacaa cggcaatgtt tgctgcagtg
     5941 gtcgatttcc cttctccagg tcctgatgaa gtcacaacaa tcgttttgat ttgctgacca
     6001 gctgcggagg caaactggat attcgtccga atcgtacgat attgttcagc gattggtgaa
     6061 gagggattgg tgagtgtgac caaactgact gcatgagttt gggctgcttt ttgtttctgt
     6121 gttcgtgcca ttttctttcc tcctagactc ttgagcggct tctgcgtgat ggcgctgttt
     6181 tatcgtttgt agcttttggt tgtgtctgag ctggtccttt tttacggata ccagcattca
     6241 attctttcgc tgacatttct ggaactgttc ctaagattgg gaatcctaat gtatctgtca
     6301 catatttttc atctttcact gtccgatcga gtaattccaa taagaatgcc agacctacac
     6361 ctaccatcat tcctaacatt aagccagtca ttaaattcaa tttattattt ggcgaaacag
     6421 gtgtcgtatt ggcaactgca cctgaaatga tcgagatttt atcaacattc atcacatctt
     6481 ttgcgttctc ttggaaaact tgtgaagttg tgttagcgat ctgttgagca gccactgcat
     6541 tcgtacttat tgcctgaatc gagaacattt gtgaattttc tgcctgatta actgagatgg
     6601 catctttgat ttgttcagca gtcattttca cattgtcttc tgtttctaaa cgatctttga
     6661 cttcattgag cactaaatca ctgacgatca tatctttata cgtattgatc atttgcaggt
     6721 tcgtattgac atcattgaca tttgcagtgt ctgattgtgg cagtgtcacg atcaattgtg
     6781 cttgtgaact gtattttggt gtcaagatga aaaatgttac gattcctgag atggccaagc
     6841 cagcaaacat tgagatgatg attattacca agtgtttgcg taatgtttca aaaatttctt
     6901 tcaagctgat tgtctcttcc attttttctc cactttccta tttattctct agattcattt
     6961 gatcttttaa ttcttttttc acacgatcaa gttcttgatc actgactttt tgataggaga
     7021 tattatcttg catgaagcct tctccttgca gctgatcttg cttaatcgta ccaaatgcct
     7081 cccgataatc taaagcaatt tttttcattt catcaaagtt catgtctgtt ttcatattcg
     7141 actctagagc attcaaaacg gattgatagt tgctgacacc gtttagactc aatacttttt
     7201 tcgctacgcc ttctacgatc tttctttggc gcccttgtct accgtaatca ccatttgggt
     7261 cttcataacg catacgagaa taagataatg cctgatcccc gttcaatgta attttaccga
     7321 ttggaaagtc atagccatct tgtgagaaag tgaggtcatt attgacttcg ataccaccaa
     7381 ctgcatcaac gagttccttt agccctttca tattgataga gacatagtga tcgattggga
     7441 tgtctacata tttttgtacg gtatccatcg ccatggcagc tccgccgaat gcgtaagcgt
     7501 ggttgatctt gtcttttgtg tcacgaccta cgatgtcgac atacgtatca cgaggaatgc
     7561 tgacgatcgt tgtctgtttg tcttcaggac tgactgtggc aaccatcatc gtatctgatc
     7621 ttccttgatc gacccgacct aaatctccag tatcaatccc catcaataga acagagaaag
     7681 attcttgttt gtccagatca actggtttcg ttcgttctcc ttgggttcgt tccactgatt
     7741 cgtaagtttt ctcaatagaa tgtgagagat ccagatataa ttttgctcct actcctacaa
     7801 ttgctaatac taatacgata agaactgcta aaataatcag aaatattttt ttccctcttg
     7861 attttcttct tcgtcttgcc atagtttcct ccgtttgcta accttgcttg aatcaacgtt
     7921 ttaactgtca atagcttata ttgctatagc ccatttgctc agcaacagtt tgtgcttttg
     7981 ttgcgtatgc tgcgcttatt ttttcattcc ggatctgctc tatttgactg agcaccgtca
     8041 aatattgatc gtaagtagcg gtggcagtca cttgaccatc ctttaacatc gtatcgaata
     8101 actgatctag ttgcgtgact tgcgcgatct gaatatcagc gaatcctaga tattgcataa
     8161 caaccgtttt ccaaggacta tctttaaaaa agctcatatt
//
LOCUS       DARBFO010000106         8174 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000106 DARBFO010000000
VERSION     DARBFO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.109"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            118..510
                     /locus_tag="SCK51_002532"
     CDS             118..510
                     /locus_tag="SCK51_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253105.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(683..865)
                     /locus_tag="SCK51_002533"
                     /pseudo
     CDS             complement(683..865)
                     /locus_tag="SCK51_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            882..1049
                     /locus_tag="SCK51_002534"
     CDS             882..1049
                     /locus_tag="SCK51_002534"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253106.1"
                     /translation="MENTFELYNKTTVDKLIPILVPTDLNGHDVLLRFNGFLGEDYRY
                     LKISDPQPLTE"
     gene            complement(1092..1667)
                     /locus_tag="SCK51_002535"
     CDS             complement(1092..1667)
                     /locus_tag="SCK51_002535"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEG1253107.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1822..2348)
                     /locus_tag="SCK51_002536"
                     /pseudo
     CDS             complement(1822..2348)
                     /locus_tag="SCK51_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2805..4316
                     /gene="gadC"
                     /locus_tag="SCK51_002537"
     CDS             2805..4316
                     /gene="gadC"
                     /locus_tag="SCK51_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HEG1253108.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            4329..5729
                     /locus_tag="SCK51_002538"
     CDS             4329..5729
                     /locus_tag="SCK51_002538"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HEG1253109.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5894..7334)
                     /locus_tag="SCK51_002539"
                     /pseudo
     CDS             complement(5894..7334)
                     /locus_tag="SCK51_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            7727..8174
                     /locus_tag="SCK51_002540"
                     /pseudo
     CDS             7727..8174
                     /locus_tag="SCK51_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT         2611 a         1340 c         1466 g         2757 t
ORIGIN      
        1 ttcttttaag taaaaaatat aggtgatttt ttttgacttc atatttataa taaagacacg
       61 taaatcaatt atttttcatt ttactttaac taataatgtt tatttggagg gattattatg
      121 ttagatgata gattgacgtg ttctaattgt ggtacattat tttactatga ggaagttaga
      181 aatattgtaa aacatagaaa aaaagaaatg cctatacgtt gtccaaattg caatgaagtt
      241 attaagcgaa agttgtctca tggttatttt gtcagctacg aactatatga agaaagtaaa
      301 ataaatctga aaaaaaaacc aaaatactat aaaaatttga aaactggagc tattataact
      361 gaaaaagaat ttaaaaattt agtgactgaa gaagaaagca ggttttataa tttgtctggt
      421 aatatttgcc ctggatttat ggatcagatt attgaaaatg agtcaattga atcaattgaa
      481 aactatgttc ctgttgactt accaaaatag aaaaaataat cctgttttta cagtgaatta
      541 ttttaatttt gattttcaat aaaaatatat tacaataata atcgtaatag ctaatgaaac
      601 aatttctcaa tctttttacc actccaaaca gggagcgtca ataattttgt gtaaataact
      661 cgtccttctg caaaataatg ggttactcag taaacattga agctaatgta tccgtcactt
      721 gttggaagcc tttatggctt ctatttaaga atttttgatt gtatgtatca aagatggata
      781 ctaggaaacg ctctagtgat tcttcatttt gaaactgctc ttttctgcgg ctgtatttct
      841 tgatttgttt attgaaagat tcgattgtag aaccagaagg aatggagaac acttttgaac
      901 tttacaacaa aactactgtt gataaattaa ttccaatttt agttccaact gatttaaatg
      961 gtcatgatgt tttacttcgt tttaacggat ttttaggtga ggattataga tacctaaaaa
     1021 tttctgatcc tcaaccttta acagaataat aaaaaacgtg taatcaatta tttgattaca
     1081 cgttttttat ttcaatcttc gttgtagtag tggttgcgac ataccgaaag gtaatcttca
     1141 ttacctccta ctacaatctg ttcacctgaa taaactggaa taccatcaac aatacgagca
     1201 ttcattacag cctttttatt gcaaaaatgg caaatagttt taatttcctc aattttatca
     1261 gcaaatgtca ataatgcttc ggatcccttg aagagattat tttgaaaatc attcttcaat
     1321 ccatagcaaa ttactggtac ttcctcattc attacgataa gtgatagatc aataatattc
     1381 tctctactta aaaattgaga ctcatcgacc aaaatacaat caacttttcc gtactgatca
     1441 ataatctcca ctaattctcc gtacacatcc atttcagaag taattgcaat agctggtttc
     1501 gaaacaccta tacgactctc aacttttcca aatcctgatc gagtatctat tgcgcttgtc
     1561 attatgataa ctctcttccc ttgttcttca taattatgag ccactttcag tagctcaata
     1621 ctctttccac tattcattgt tccatatctg taaaataatt gtgccattaa atttattccc
     1681 ttcttttaca attcaattat tccttaattc catctgcttt tatgcagctg ctcgtttatt
     1741 ccttgatatg tctataacat ctactatccc taaaaccaat ttataagcca ttagagatgg
     1801 tattcctgaa catctctcat attgtttttt gagtggtata tcatctgaaa aactgaagga
     1861 atcatgaaaa tagcgacata cttatcattt agagttctaa ctttaaatgc atttacttct
     1921 tcagtcagca ctttagtcat gttggacatg gtttggggcg tgtagtgatg cccatacatt
     1981 ttctcaatca agtctgcgat ttccgacatg gtaatacctt ttcggaagag gtgaatgacg
     2041 gtctcctcta atgtgtcatt cgtccgtcta taagcaggaa cagtctgttg cttgaactcg
     2101 ccattgcggt cgcgcggaat ctggagatga agttccccat actcggtctt gaccgtacgg
     2161 tcataggagc cgttacgaga attacccgtg ttaaaaccaa tgcgatcgta cttttcgtaa
     2221 tcgaggaaag ccgttaactc cgttttgaga agcgtattga cagctttttc caaatggaca
     2281 cggaataatc cattcaaatc gcctttgttc gctagagtct ttaggatttc tgtagtaaaa
     2341 tcgttcatat ggaagtcctc ttttctgtga atgtgttgtg gtaactctat tctacagaag
     2401 ggacttcctt ttttctattt acacaaaata ttttacactc tcaacaaaag tatcttcgat
     2461 taatacttgc tcaagttatg tgcagctacg aaaaagtagg taccgaggta ctttcttttt
     2521 cgcagctttt tttggactta tgagaagtac attcgctgga agaatacgat ataaaacgag
     2581 ctttttcact aaagatatta atagagtgaa agaaacaagc ttttgttgtc agttttttca
     2641 cttttctaaa aatgaaaata aaaaattctg gaagtggaga agaattttgc ttgctatact
     2701 ttgtcttgta gaaaagaggt ggacaaaata atgcaagctc aaataagcga ttttccataa
     2761 ccttggtagg aatgacaaat taaataggaa agggaagtaa taatatgaat aaaaaaaagt
     2821 tatctctatt cggttttttt gccctgacag cttccatggt tttgactgtt tatgagtatc
     2881 cgacctttgc aacatcacaa ttacatttgg ttttctttct tcttttggga ggtattttat
     2941 ggtttctacc agttgcttta tgtgctgcag aaatggcaac agttgaagga tggaaagatg
     3001 gtgggatttt cagttgggtt agtcaaactt taggagaacg ttttggtttt gcagctattt
     3061 ttttccaatg gtttcaaatt acagtaggtt ttattacaat gatctacttc atactaggag
     3121 cgctgtcgta tgtaattaat tttccagagc ttaatacaga tccatttatc aagtttattg
     3181 gtgtattagt cattttctgg gtactgactt tttcacagtt gggcgggacc aatcgtaccg
     3241 ctaaaattgc taaagcagga tttgtcattg gaattgttat tccttcgatc attctctttg
     3301 gtttagcagc agcctacttt atcggcggta atccgattca aatggctatt aatgcgaaag
     3361 cttttattcc tgacttttct cagctttcaa ctttagtagt atttgtatct tttatattgg
     3421 cttatatggg cgtagaagct tcagcatcac atattaatga gctggaaaat ccccaaagaa
     3481 actacccatt ggcaatgttt ttactagtga ttttggccat cgccttagat gcaatcggcg
     3541 gtttctcagt agcagcggtt attccacaac atgacttgtc tctcagtgct ggtgtaatcc
     3601 aaacatttga aacgcttatt ttacatttta acagtcattt acattggttg gtaaaagtga
     3661 ttgctctcat gattgcctgt ggcgttatgg gtgaagttag ttcttgggta gttggtccat
     3721 cacgagggat gtttgcagct gctcagcgag gtctgttacc agttttcttc cggaaaacta
     3781 ataaacaaga cgttccagtt ccgttgatta tgatacaagg aattatcgtt actatttggg
     3841 cagcagtctt aacttttggt ggaggaggca acaatttatc attcttagta gcaatctcct
     3901 taactgttgt catttatctc gtaggatatt tactattttt cctaggttac tttgtattag
     3961 tttttaagaa aaaagactta aaacgaactt acaatgtacc aggaaaaact gttggcaaaa
     4021 cgattatcgg tggaattggt tttattttat cagtgttcgc attgcttatc tcttttgtac
     4081 caccagcttc aattgcagct agtgagcacc gaacatacca aatcatacta cttattagct
     4141 ttgtagtgac tgttctttta ccttttatca tttatgaagt acatgataaa agtgctcaca
     4201 caactattga ggaacctact cacgtgaaag ctgaagacgt taatcccgct gtttatccag
     4261 ctgctagagg agagcaccac gttacaaaaa atgatgaaca tattttaaaa cattaggagg
     4321 aattcattat gttatacgga aaagataatc aagaagaaaa aaactatttg gaaccaattt
     4381 ttggctctgc aagtgaggat gttgacttgc ctaaatataa gttaaacaaa gaatccattg
     4441 aaccacgaat tgcttatcaa ttagtacaag acgagatgtt ggatgaagga aatgcgcgat
     4501 taaacttagc tactttttgt caaacgtata tggaacctga agcagtgaaa ttgatgaccc
     4561 aaacgttaga aaaaaatgca attgataaat cagaataccc acgaacaacg gaaattgaaa
     4621 accgctgtgt aaatatgatt gctgatttat ggcatgctcc aaataatgaa aaattcatgg
     4681 gaacttcaac gatcggctct tcagaagcct gcatgctggg tggtatggcc atgaaatttg
     4741 cttggcgtaa acgtgctgaa aaattaggtc ttgatattca agcaaaaaaa cctaacctgg
     4801 tgatctcttc tggttaccaa gtttgttggg aaaaattctg tgtatattgg gatgtggaac
     4861 tgagagaagt cccaatggat gaaaaacata tgtcaattaa tctagatact gtcatggatt
     4921 atgtggatga gtacacaatt ggtattgtag gtattatggg tattacttac actggtcgtt
     4981 atgatgatat caagggtctg aatgatttag ttgaagctca caataaacaa actgactata
     5041 aagtatacat tcatgttgac gctgcatcgg gtggctttta tgcaccattt actgaacctg
     5101 atctagtttg ggattttcaa ttgaaaaatg ttatctcaat taattcttca ggtcacaaat
     5161 atggtttggt atatccaggt gtgggttggg tcttatggcg tgaccaacaa tacttaccag
     5221 aagaattagt atttaaagtt agttacttag gtggagaaat gccaactatg gctatcaact
     5281 tctctcatag tgcagcacaa ctaattgggc aatactacaa ctttgtacgc tatggctttg
     5341 atggttatcg tgatattcac caaagaactc atgatgttgc tgtttattta gccaaagaga
     5401 tcgaaaaaac tggtattttt gaaatcatta atgatggatc agaattgcca gttgtgtgct
     5461 ataagctgaa agaagatccc aatcgcgaat ggacactata tgatttatct gatcgtctgt
     5521 taatgaaggg atggcaagtc ccagcctacc cattacctaa agacttggat caattaatta
     5581 ttcaacgctt agttgttcga gcagactttg gaatgaacat ggctggtgat tatgtacaag
     5641 atatgaacca agcaattgaa gagttgaata aagctcatat tgtttatcat aaaaaacagg
     5701 atgtgaaaac gtacggcttt actcactaaa attcttcaga gaaatataaa agaagttaat
     5761 taactaataa aaggagttgt gattaccaca cagctcctta aatctatttg aataaatata
     5821 tgatattcat ttgttacact ctcaatctta gctgttgttc tattcatcat tactcttatt
     5881 gtaagtagtt cagtcaaatt tgcttgttca attgatcgat aacagtatga attgtttgaa
     5941 aagtaatcgg atatataatt gttttctcac tacctaagcc ttcttggtat aaagaactat
     6001 cgcaaagtat aatccgctga gtacaaaaga aagtgtcgtc tactatttct ctaagttgtt
     6061 tacaaatgat attactaata gaaaagtgtt ctcccaaaat gtcttctaac tgttgtcgat
     6121 agtataaata cctaaagtct gacggagcta ctatgaacac ttcaattgga gatttccgtt
     6181 tatgctcatt taaaagactt aaactaatgg ttagtttacg aatagaattc tcattccaac
     6241 gcagaaataa attgttttgt tttcgccatt gttcaagaat ttcatgaact tctaagtaca
     6301 aagcgcgttg ctcttcattt aacaagtaag tcttatcagg ttgaaatatc tgaatatcag
     6361 cccaaagtga gcggataaac gggagaagtg ctttatcaaa aagtatctga ttttcttcag
     6421 atatattcag agataagata agtttttgac tgagctcaat caccaaaggg ttatctgcta
     6481 catggttttg ataaacaacg ttcaagtctt tttgtaacaa ttcaaagtta tcgcatagat
     6541 aatttctaga gttgaataga aagtagatat aaaggcattc atcttcgtca tgacataaag
     6601 agaaagaaag ttgctctaat cctttctgta ttagctgaaa caagggagta tgtcgggcaa
     6661 ctcttttttc ttcctgacta aaatatattg ggttagtctt agatctctgc aaaccgatag
     6721 cgatacctaa taaaataaat tgtttttcaa ctgtagggta acttcttgaa aaaaaatgtt
     6781 tttctacata atcaatcaat tgttgaccgc tttgaataat tgatttattt agctggatca
     6841 actctgtccg atttgtctca tagattatcg ttagtaacaa acttcgataa tccttttcag
     6901 gaatttcaaa atggccatca gcatttaagt actgcatttg tttaaaaaaa ttaatcaact
     6961 catttcgtaa gttgtaaact ttgcttaacg aaatgaaggt agtatcagct atattactcc
     7021 aattgtgttc ccctgataaa taatgtccta gtacattgaa aaagtcagag cgctgataga
     7081 tgagttgagt gagtgccaac ctcgaaaacg aagagtcgaa tgcgactgta aagctacttc
     7141 tagatttctc cactgaaata atttgctgat ggagtatgtc ggtaatgcat tccaagtcgt
     7201 tagtgacaga cagtgagtgg gcaaacgttc aattgatctg ctatttcgcg aatctgaatg
     7261 gtcccttttt cgtgtaattg ctcacaaatg taaacttgac gaaaaatact tttctccaga
     7321 taggtttcca acatagtact cctcctagtc attttataca taactattat aactctacca
     7381 atcggaatag tcctgtaata tattgaatca aaaatataaa ttataaaata attagatggg
     7441 gtatttggtt taagtatccc ttatatagta ataaatttat aggtgtgatt ctcgaattaa
     7501 tacccgaaaa tgtgagagaa tttttatgga ttttttagga tacaaattaa ttatttttgc
     7561 ttttatcctg attaccagtc cagtactgtc ggtatcccat tttgcagcaa aatcatgttg
     7621 ataaagtgac aagaagactg atgagatata ttatccaaat tgagaagata attaatcgga
     7681 ttcttgagta taccctactt tttaagaaag tagggtatac aagcgtatga ataaagacat
     7741 aactttccct gtgatggacg ttcggcttgt tccaattacc aaaatacagg cgaacgatta
     7801 taatcccaat cgagtagcgt ctccggaaat gaacttattg gagctttcta tcattgaaga
     7861 tggctttact caaccattag tttgttatta caaggaaaaa gaagagaagt atatcttggt
     7921 tgatgtgctt cataggtatc gtgttgctaa ggaaagactc aacttaaaag ttgtaccggt
     7981 aacagttatt gataaaccca tataagaacg tatggcttca acaattcgac acaatcgtgc
     8041 aagagggacc catgaaataa gaggaatgga aattgtcatc caaagattag taaaaaatgg
     8101 atggtcagac aagagaatat ccaaagaatt aggcatggac attgaagaaa tttttcgctt
     8161 taagcaagtg tctg
//
LOCUS       DARBFO010000107         7782 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000107 DARBFO010000000
VERSION     DARBFO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.110"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..338
                     /locus_tag="SCK51_002541"
     CDS             <1..338
                     /locus_tag="SCK51_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253110.1"
                     /translation="SVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKS
                     ISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNN
                     NFYFYRNKL"
     gene            complement(464..1141)
                     /gene="nth"
                     /locus_tag="SCK51_002542"
     CDS             complement(464..1141)
                     /gene="nth"
                     /locus_tag="SCK51_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEG1253111.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1148..1846)
                     /locus_tag="SCK51_002543"
     CDS             complement(1148..1846)
                     /locus_tag="SCK51_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEG1253112.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2114..3298
                     /locus_tag="SCK51_002544"
     CDS             2114..3298
                     /locus_tag="SCK51_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEG1253113.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3304..3747)
                     /locus_tag="SCK51_002545"
     CDS             complement(3304..3747)
                     /locus_tag="SCK51_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1253114.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3973..4260)
                     /locus_tag="SCK51_002546"
     CDS             complement(3973..4260)
                     /locus_tag="SCK51_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253115.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4431..5783)
                     /locus_tag="SCK51_002547"
     CDS             complement(4431..5783)
                     /locus_tag="SCK51_002547"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEG1253116.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6056..6502)
                     /locus_tag="SCK51_002548"
     CDS             complement(6056..6502)
                     /locus_tag="SCK51_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG1253117.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6738..7664)
                     /gene="cysK"
                     /locus_tag="SCK51_002549"
     CDS             complement(6738..7664)
                     /gene="cysK"
                     /locus_tag="SCK51_002549"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEG1253118.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2105 a         1583 c         1270 g         2824 t
ORIGIN      
        1 tttctgtact tttaagcact actcttaaag gaaaatttat ggaaagaagc tctcatgcaa
       61 tgattctcac ttttttatgg ttgttgttca ccagaatcta tatttttctg atgagcctga
      121 aaccgcccaa atccattagt ttatgtttag ttataaatgg tattttttta ttatcactta
      181 tcatttttat tacaccttta gatagatacg tcacttattt atataatcct ttggaatact
      241 ggacttattt tataggtata ttggaaggca ttattttcta catcgggtat tttcccaata
      301 aaaataacaa cttttatttt tatcgcaata aattgtaata agttaccatc ctagctactg
      361 agtatgtaca tttacttttt catgcaaaaa agataaacga aacagctttt ctccataata
      421 acaagataat tcatacattt atttgcaatt aaagaaacca cgtctacagg cgtggtttct
      481 tttttagcgc cttttctttt agacgcatcc gatttttccc atcttgacat attgatagca
      541 aaggacatac ctcgcacttg ggatttcttg ccgtgcaatg atatctacca aaaaaaatca
      601 gtgtatgatg tgtctttacc cataattctt gtggtacttt ccgcatcagt gtttcttcta
      661 cttccatgac cgtagcatct agtttgcaga tcctcaggcg ttttgagaca cgttccacgt
      721 gggtatctac tgcgattgct ggtatcccga atgcatcccc caatacgaca tttgctgtct
      781 ttctgcctac accaggaaga gacatcaatt cttctcttga cgttggcact tgcccatcga
      841 aacgttcaat cagctgctgt gcacacgctt tgatgttttt tgctttattt cgatacaaac
      901 caatcgtttt tattttaaga atgatttcat cgattgaagc ttctgctaac gcatcgggtg
      961 tcggaaaaga ggcaaacaaa tctggcgttg ctttattcac tgatacatct gtcgcctggg
     1021 cacttaagat cactgcaatc aataactcaa atggattatt gtgcttgagt tctccatgtg
     1081 cttcgggaaa cattccatac atcgtttcta gagcttccat tgttttttgt ttgcttaaca
     1141 tattccgcta ctcctccaac caatttttca gtgagacttt cggcaattct tcttgttgag
     1201 ctgactgttc tacttctctt tgaagaagct gctgttttct tcgtttttgg tcttcttcca
     1261 cttgttgttt tgtacgtaga ttttttcttt cccacgatag caaaatgcgg tcaacgtaat
     1321 taaaactata agcctgattt aatacggctt cccgcagtgc tagcttcaaa atttccggct
     1381 gataatggtc ttcttctagc cattgtccga tccgctggaa ctcgatcgaa gaaagcgggc
     1441 gtccaaattc ttgttcaaat aattgataca ctgactggat ttctttttct tgtgtttttt
     1501 cttcgtattt tttttcttgt gtttttaaat actcacctaa tagatcatag atggggtaaa
     1561 gattatatcg atctgctttt ttaccgttga caatcgtcgt ttctatcttg ataaatccat
     1621 ttgttacgag acgatccagt atttgaaaaa tctgatcttg tttgattccc atatccatag
     1681 caatcatctg caaatcaggg aaagtatctc cttcaagctg tgccatatgc aactgtagaa
     1741 caaataaaaa ttcttcagat tgtagaccga tcctatgata atttttaagt aaaagattag
     1801 atatgactgt ttgtccagcg cttaagtaat tttctaaatt caacatgatt tctcctccgc
     1861 tcaatcacag tatacgtaaa aaaaacatgg gaagcaagaa caggtttctt ttctatttac
     1921 ttcactgatc ggtcagattt acaatgtcgt ctacttgatt tttgtttgtt catgtttttg
     1981 atgcgtatct tcacttttcg gaataaataa attaagtaga tttcgctgtt ccttagaatg
     2041 ttgacaagga aacaaaatta ctatacacta tttttgtttc caagttaaca ttattgcata
     2101 aaggagtttt tacatgtaca gtttcacaaa taaacattct aaagtttttg gatacgtctc
     2161 tgtcttattc actggattag gatttactat cgtcagtcct gtacttccgt ttttaacgct
     2221 gccttattct cactccatac atcagcaagc tttctatatc actttgctca tgtctgttta
     2281 tgctctagct gccttcttat ctgcccccat tctcggtagc ttaagtgatc attttgggcg
     2341 tcatccgatc cttattatca gtttattagg atcaagtatc ggatatttgc tttttggttt
     2401 aggaaattcg atatggatgt tgttccttgg tcgtatcatt gaaggattga cggcaggaga
     2461 aatctccacc ttatatgctt actttgccga catcacggaa cctaatgaaa gaaccaaagt
     2521 ttttggatgg atgggtgctt tagtcggtat aggaacaacg ctgggtccta ttataggggg
     2581 tctattagct gagttgggga attctgttcc tatatttatt ggagcactct ttactttctt
     2641 aaatgccgtt tatggctaca catttatgct agaaagcctt ccaataaaaa aacgttcagt
     2701 agatttaagt ttctcccatg taagaccttt tcaccaatta caacaattat tcaagatttc
     2761 atccgttatt cctttattga cagcaggttt tgctgtttgg ttggcagctg gctctcttca
     2821 atctatcttt tctcaatttt cgattgacgc ctttcagtgg aaggctgggt tggttggttt
     2881 aagcttctct ttgattggaa tcttggatac tgtctcgcaa ttgttcatta tgcctcgatt
     2941 gctcaaaaaa ttttcagaac agcagatcac aagaataggg atgttcagtg aaatcctcgc
     3001 ctatctattc atcacattat ctggaattct tctactgccg attttgtttt tatttgggat
     3061 tatctgttat ggtttcggtg attctatttt tactcctgta ttcaatggcc aactatccaa
     3121 ttctgtctca gataaccagc aaggtcttgt aatgggcggt acccaaagta tacaatctct
     3181 atcgcgcgtc atcggcccgc taatcgcagg acagctttat gctgcagcac catttcttcc
     3241 gacggtattc ggatttttgc ttatgatttt tgcctattat cactacaata aaaaatagta
     3301 gcttcatttg ctacttcgta taaattctct gtatcgaccg ataaaacgaa atactgtgtc
     3361 aaattcttct tttgacatat tttcaaatac agtagcatct cgctgtaccc atttttgatg
     3421 gagttgctcg tgtctattcc ttattttttg tccgattggc gtcaattgaa aatagatttc
     3481 ttttttattc tcttcgcttt gataagtgtt tatgatccct ctagctatca gtttttttgt
     3541 tagcttactg atggctcctc tagtcatgta actagcattc gctaattttg tcacgttagg
     3601 atcagggatt ttcccgatat gttcaatcac ttctagttca tttagagtaa atcctgcaaa
     3661 ttctttttcc atttctggtt tattacgatg aataatctca ttagctaaat ccagatattc
     3721 ttctattagc agttgtcttt tatccatttc tacaccttct ttttctttca ttatattgtt
     3781 tttttgtttc ctattcaaca aagataaaaa tattttgtag gaaaacccaa tctgaaacca
     3841 agaaaaaaag gggttgacaa agcttgtcat aacccctttt tctgattaaa agatagtaaa
     3901 gagacagtca tcggtaattg tgatgatttt ttgtcatcac ttaaggatga gcggcctttt
     3961 caaaaactgt ctttaaagat catcatcagt ttctagcaca tatggctgac cttcatatgt
     4021 gtactctgta ttcaatgtgc cgtcattttc atctgagtag atgcgtctac tgaaaggaaa
     4081 acgatgagaa acattatctt tggcgtcatt tgctggcatc gtgtaatatt cctcttgcgt
     4141 ttcattcaac tgagagagta cttgatctgc caactcatca atattgatcg ttaatttctt
     4201 cgaagcactt ttataatctt ctaaaaatgc atcttgttgg acaaaatatt ctttattcat
     4261 cttctttctc acctttcttt tcaaaatcaa ataaagctgc ttgttctttt atgtaacagc
     4321 tttatctttg ctgcttattg tgacaaaaaa ttcgaagaaa gacaactaat tgtacttttc
     4381 acaaaaaaag acagagggtc gtgacaaaat tgtgtcacaa ccctttttct ttattgtgca
     4441 gtttcgattt cgcctagcgc tgcttgtgca agtaggttca agtaattcca tgggcggtca
     4501 aaatgtggtt ggaagaagaa atctgaaagt gcgagatctt ctacagtcat tttattttga
     4561 acagctaaag acatggtatt tgctgattgt gtaatgtcgt atttggacat gaactgagca
     4621 cctacgatac gattcgttcc tttttcatag actagctcca tcattacttt ttcagtagtt
     4681 ggcatgaact ctggacgata attatcttca aatacagtcg cttggacttc tagatcgttc
     4741 aaagttgcgc tttctttggt cacacctgtc gaaccgatcg tccagccaaa taggtaaagt
     4801 cctgaagttc cttgagtacc acgatacgcc attttatgat ctgtcaagtt atttcccaca
     4861 agcaaacctt gacgaacggc atttgttgcc aaaggaatat agttttgcgt gccacttgga
     4921 ttgtagttga ctactgcaga gtctccagct gcaaaaatgt ctggattact tgtctgcatg
     4981 tattcattca ctttgattgc cccgtttggc agtgtatcta cttttccttc gatcagtttt
     5041 gtatttggac ggaagcccac acataaaatg accatatctg tatcaaatgt atctgttggt
     5101 gtagctactt gtttcacttt tccttcttca tctgtgataa attcagtaac attttcgcct
     5161 agcgcaagag taacaccgcg gtctgtcaat tctttttcta agatatctgt gaatggttta
     5221 tctagatatt tattcaagat gcgatctaat ccatcaatca atgtcacttg ttttccagat
     5281 tcgacaaaag cttctactaa ttcaataccg atatatccac caccaacaat tactacacgt
     5341 tttgcttctt tcgcttgtgc aataatttca tttgcttgat tgtagttttt gcataggagt
     5401 acatttttcg catcgattcc actaatcggt gggataatag gccatgaacc agttgtcatc
     5461 actaatttat cataagaaac cgtttctgtt tctcctgttt tcagatcttt tgccgttact
     5521 gttttatttt ctgtatcgac gtctgttaca tcgtgttcca ttttaactgt tgctcctaat
     5581 gaagataatt cttcaggatt tgagtagaac aagcctgctg ggtcctttac tactccgccg
     5641 acataaagtg cgataccgca agataagaaa gaaatattgt catttctttc atatactgtc
     5701 acttctgctt ctggatgatt ttttaaaata ctttttacag ctgcagtacc tgcatgagta
     5761 cagccaatta ctactacttt catttatggt ccctccgatt aacattttgt ccttcgacaa
     5821 aactactata acagaaataa aacggataca acagtttaat gcttgtgaat tcacaaaggt
     5881 ttgtttgtag tttcattcct tttcaagcat aagaacccaa tatctacaag attcttattt
     5941 taataatatt cacttttata tctaagattg tgaataaaag acaaaaaaat aaaaaagcta
     6001 gcgttttttt tacacagcta gcttttttta ggtataacac ttgtagaaac tgtttttaca
     6061 tttgcgcctt ttttgctgac tctttacaat ccggacagag tccatacaat tccaaccgat
     6121 gttcattaat ctcaaaatca gtcagtctgc ttgcagccat ttctatatct tcaagccctg
     6181 ggtaatgaaa atcaacaatc ttaccgcagt tttcacaaat tacatgataa tgacgctttg
     6241 aactgaaatc aaatcggcta gaagcatcgc cgtacttcat ttcttgaaca aatccaatcg
     6301 aagtaaataa gcgaaggtta ttgtaaacag tcgcaacact catatttgga aaccgatgtt
     6361 caagtgcttt ataaatctca tcagccgttg gatgggaatg atgttcaatc aaatattcta
     6421 aaaccgcgta ccgttgaggt gtgattcgaa tgttctcttt ttttaacttt tcgataacct
     6481 cttcgactaa cttattttcc atgtaaacac cccacgttgt tattctcgtc ttatcatact
     6541 atttttacat tttaaaatcc acttaacatg aaaagaaaat tcaattttat tgtattttaa
     6601 tgaaaaaaga cgtaaaacag tcgcgttcac tattctacgt ctctattcaa ataatttttc
     6661 tcatcataaa tttttgtgtg ttcttttcat ctttaaaagt tttttacata aacctatctg
     6721 gagaaaaacc aagtgcctta tatctcttgg taaagggctg tagataaata acgttcacca
     6781 ttgtcgggaa ccacagctaa taccttcttg ccttttccta attcttttgc gacttttagc
     6841 gcggcagcaa ttgcagcgcc agatgagatg cctactaaaa tcccttcttt tcgcccaact
     6901 tctctagctg tttccatcgc ttcgtctcca ctaatagaaa ggactttatc atacacagaa
     6961 gtatccagtg ttttaggaac aaaacctgta ccgattcctt ggattttgtg aggtcccgct
     7021 tctttccctt ctaaaatagc agattctgct ggttctaccc cgattaattc gattttcgga
     7081 tacacacgtt ttaattctcg gccagcgcca gtgatcgttc ctcctgtacc gatacctgca
     7141 acaaacgcgt ccaagccatt tactccaaat gcttgatgta tctcaggacc agtcgtcttc
     7201 tcatgcacaa gaggatttgc ttcattttca aattgtaatg gtaaaaagta gccgttttct
     7261 tttgccaaac gttctgcttc tctgattgat ccagaaatac catccgcacc aggagtcaaa
     7321 atcaactctg cgccgtaccc tttcatcaat aaacgtcttt cgatactcat cgtctcaggc
     7381 atcacgatga ttactttata tccttttgca actcctacca tcgacaatcc gattcctgta
     7441 tttccagaag taggttcaat gatagtatca cctggtttca gtagaccatc gtgttctgct
     7501 ttttctatca tacttaaagc aatacgatct tttacacttc ctcctggatt aaaaaactca
     7561 agctttacaa aaacatctgc tgaatcttct ggtacgattt tatttaattt gacgattggc
     7621 gttttgccaa tcaattcggt gactgagtta tagatagctg tcatcttcaa ttcctccttg
     7681 tctttgctct tcttacttat cataatacaa tctgttcatt tatggcgagt gattcgtctt
     7741 tgactagcaa aaaaaagaca aagatatacc atatatggta at
//
LOCUS       DARBFO010000108         7649 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000108 DARBFO010000000
VERSION     DARBFO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.111"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..179)
                     /locus_tag="SCK51_002550"
     CDS             complement(<1..179)
                     /locus_tag="SCK51_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEG1253119.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
     gene            complement(192..1295)
                     /locus_tag="SCK51_002551"
     CDS             complement(192..1295)
                     /locus_tag="SCK51_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEG1253120.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1385..2341)
                     /locus_tag="SCK51_002552"
     CDS             complement(1385..2341)
                     /locus_tag="SCK51_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEG1253121.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2622..4556
                     /locus_tag="SCK51_002553"
     CDS             2622..4556
                     /locus_tag="SCK51_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1253122.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5013..5429
                     /locus_tag="SCK51_002554"
     CDS             5013..5429
                     /locus_tag="SCK51_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEG1253123.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5443..6009
                     /locus_tag="SCK51_002555"
     CDS             5443..6009
                     /locus_tag="SCK51_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEG1253124.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6032..7018
                     /locus_tag="SCK51_002556"
     CDS             6032..7018
                     /locus_tag="SCK51_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEG1253125.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7173..>7649)
                     /gene="scm"
                     /locus_tag="SCK51_002557"
     CDS             complement(7173..>7649)
                     /gene="scm"
                     /locus_tag="SCK51_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEG1253126.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2192 a         1466 c         1578 g         2413 t
ORIGIN      
        1 ttattgatag cggtagtcgc taaatcaaaa actagcattc caataaaaaa taaaaaagtg
       61 tttagccaat gtatctcatg aaaaaaagcg atagagaata atacccctac cgcaaaagga
      121 aatacactag ccacctttgt tcgtatttca actacttcta aaaagatttt tagtgtcatg
      181 gttcgtactc cttattttag ttcagaacgg tcacccatcg tgtaatggga gtcctccgat
      241 aatttgaagg tgtctttgat tggatcggtc acatatactt tatcgtcttt tgtcacgaag
      301 atcgcttccg tcccgttatt caattcgctt tcgacgtatt ccagtccttt tttcacaccc
      361 attgaaaaga ctgctgtaga caacccgtca ccatcaattg atttatctgt tatgatcgtg
      421 acacttgcga tatcattgtc gaacggataa cccgtttttg gatcaaacaa atgatggtat
      481 tttttgccat ctacttctaa ataacgctcg taaattccag aggtaactaa tgttttgttc
      541 cgttctttaa tagaacctaa aacagatccg cgagccatgt tcggatcttg gatgccgact
      601 gtccagtctt ggttttcgcc tcttgggctg tggcctaaga catagacatt accgcccaag
      661 tcaacgatgg cagtggtcac tccttgtttt ttcaagactt tcactacttc gtccgtaatg
      721 taacctttgg cgattgcacc taaatcgatg atcattcctt tttcttttag ataaacagtt
      781 ttttctttgt catttagttc gatcttatga taatccacta atttcaatgc ctgatcgatt
      841 tcttcttggg aaggttttct ggcgtcatca aaaccgattc gccataattg tgtgattgca
      901 ccaatcgcca tatcgaatcc gccttgagaa tcttggctat attcgtaggc ccgcttgact
      961 aacgtgtaga cgtcatcaga aacttttact ggtttgatcc cagcttgctc attcacttca
     1021 tcgatctcag aacctttttg attgatcgtg attttatcac caagttcttt tactcgatcg
     1081 aaggcaggtt tcaatgctga ttctttgcct tcatcataca cacggatacg aacgtaagtc
     1141 cctaacaaga actgttcatc agaatatgga tctttcaata tcttggtagc tgctgaactt
     1201 tcagtggttg ttttatttgc tgagttgctg cagccagtta gcaccataaa agagatgccc
     1261 agtgtgatta aggaaaatag tagttttttc cgcatctttt tcctctcttc attttgaaag
     1321 gtgagacgac tgcctaatct ggctatcgtc ccaccctcca acttttatta gtatgattat
     1381 gtgattattt ttctgttacg atattatcta cttcgattgt ttgagtgtcg cctttttcag
     1441 ctgcattcac taattgttgt gcgtacatga tgaatgaatg tgagctgtgt gtagcacctg
     1501 taacaacttc tacattagca ggtgagccat cttcttcgcc cattgctttg acgaattcgt
     1561 cgttcagagt agggatgtaa gtttttggtt ctgtaccgct ttttgctttc atattttcat
     1621 tatattctgt atcatcttgt tttgatttac cgtctttgtc gatataatca aagtttgatt
     1681 cagttacttt gccgccagca actgtcattt cgaattgcac acggtagccg ttagagtagt
     1741 tcttttcttc aagcttgtaa gtgccgtctt tcaaatcagc accattgtcg atttcgattg
     1801 tatctgtgtt accagcttga gcagcttgga tcaattgttg tgcgtagttt tggaagcttt
     1861 cagatgagtg agtagctcca gttacgactt ccacaccgct tgcgctttgt gcttttaaga
     1921 attggtcatt caactcagga atgtattttt ctgggtttgt gccagatttt gcttccatgt
     1981 ttttgttgta ttctgcatct tttgtttttg attcgccgtt ttcattgacg ttatcatatt
     2041 ttgattcagt gattttgccg tcttttacaa ccatttcaaa gactgcacgg taaccatttg
     2101 aataattttt ttcttctaat ttatatgtgc cgtcttgcaa atctccgcca gctacaactt
     2161 ttgcagatga ttctgttgtg ctttttgcag tcgaagattg tgctgtagat gattcagaag
     2221 aagagtttga gcttccgttg tcatctgcgc cgcaagcgcc taacactaga gctgaaaatg
     2281 ctaatacggc aaatccagtt acaaaacgtt ttttcttcat tttaataccc caccattcca
     2341 tttctagtcc acaatttttc tttgtgattc attatacatt ctaatctttt tagcctataa
     2401 gtcaactatt tttgataaat aacccatgta atatttcaaa cgttttcttt cagcaaattt
     2461 cgtgaaaaaa cgctttattt taaaatttga aaaaaatcca aaaggagtta gacaaaagaa
     2521 cagaacatct atataatata tggagattgt gtaataagta tcaaactaaa agaattatca
     2581 tatattcatt atatagatag tctataagga gcggatgatc aatggcaaag caaaatattg
     2641 tcgttgtagg agcaggatat gccggtgttt cagctacaaa atacttggca aagaaactga
     2701 aaaaagaaga tgtagcaatt actctgatcg atcgtcattc gtaccatact atgatgaccg
     2761 agttgcatga agtagctggc ggacgtgtag aaccagaagc aatccaatac gatctgcagc
     2821 atttgtttgc tcgtcaaaag aatgtacaac ttgtcacaga cactgtaatc ggtatcgata
     2881 aagaacgaaa aatcgtcaag accaaattag gttcctatcc ttttgatcag ctgatccttg
     2941 gaatgggcgg agagccgaat gactttggta cacctggtgt caaagcacac ggattcaccc
     3001 tttggtcatt tgaagattct gtaaggattc gggaacatat tgagaagaca gtagcaaaag
     3061 ctgcattgga accagatgat gaactgcgta aagccatgct gactttcgtt gtctgtggtt
     3121 ctggatttac cggtattgaa atgattgggg aactgatcga ctggaaaaat cgcctagcca
     3181 aagattataa attagatcca aacgaaatca cactgatggt cgtagaggca atgccgacga
     3241 tcttgaatat gttggatcga aatgatgctg caaaagctga acgctatttg cgtaagcaca
     3301 acgtggaatt attattgaat gcaccaatcg tagaagtggc tgctgatcat atcaaattga
     3361 aagacggacg caacattccg actcacacac tgatttggac agctggtgtc aaaggaactt
     3421 ctgacgctgc tgactttgga ttagaatcag cacgcggaca gcggttgatc gccaatgaat
     3481 atatgcaggc aaaaggctac gaagataaaa acatctatat cattggtgat ttagtctttt
     3541 atgaagaatt cccaggaaca ccgacaccgc agatcgttca agctgctgaa caaacaggac
     3601 acacagccgc agcgaatatc gtggcttcga tcaaaggcgg agaaaagcat aaatttaaag
     3661 gaaactatca aggatttatg gtttctattg gtgccaaatg gggggtagct aacctatttg
     3721 ataagatcca tttaagcgga tttttagcta tcgtaatgaa acatatcgtg aatttgaaat
     3781 acttctttga tattcgttct ggttattata tgttccaata tttaatgcat gagtttttcc
     3841 atatcaaaga tgatcgcaac gtgactcgag gacattcttc tcgttacggc aatgtcttat
     3901 ggagtgtgcc acttcgtatt ttctatggtt tagtttggtt cgttgaagct tccaaaaaaa
     3961 tcattgggaa tggtgaagtg ttcaaaccaa gtacttggtt tggtgaagga tcatggttca
     4021 ctggaaacag tgtgttccct tggacatggg aatacaatgt aacagatgtt gccagtggtg
     4081 cttctgctgc tggtgatgca accacagctg ccagcggagc aggggcaagt gccggagaag
     4141 cagcgactca agctgcacat tttggtttga gttatgcata cggtgaagaa ccaatggctg
     4201 tgattgagaa gtcaccagac tggttcacaa agatcatgca agtgatcatg ccaaacaatg
     4261 atgtagcaat gttcttccaa aaattcatgg tattctttga aattgccttg gcattagcat
     4321 tgattgctgg attattcaca tggctatgca gtgcgacaac gattgtattg gtgatcacgt
     4381 tctgcttatc aggcatgttt tactgggtaa acatttggtt catttttgtt gctttcgcat
     4441 tgatgaacgg ttccggacgt gcgattgggt tggatcgctg ggtaattcca tggctacaac
     4501 gtacactagg taattggtgg tacggtagac caagttccca gtacggcaag aaataaatag
     4561 ataaagaacg ttatttgaat gcaagatttc aatagaaagg ggctgagaca aaccgtcaca
     4621 gcctcctttt tatgtaaaat taaaggaata agatgcccga gataagcctc catgagcaag
     4681 acacaggtgt cacaacctct gttacaacct cttgttataa acggtgttcc agaagtagct
     4741 tcttcgaaaa ataagccgaa atttcacaaa aacttgaaaa gtaattttcg taaattcctt
     4801 cttatttctt gaagttgccc acttctgtca caacctcttg ttataaacgg tgttccacct
     4861 gctgcttctc ggtaataagc ccaaagaatt agaaaacatg gaaagctgtt ttctattctt
     4921 cgttcttatt actcaaagca agacacaggt gtcacaacct cttgttttta tgctaaaatt
     4981 gggtgagtta atctggaaaa aggagaacaa aaatgagcct taaagaattt gctgaaaaaa
     5041 gtctgctcaa accttgggat gtttttatta ttttgctttt gactgttctt tcttttttac
     5101 cagtcatgat ttttggtctt cagcaaaaca ccgcaacagc agaaaaagaa gcggtactgc
     5161 gtgtggatgg cacagaaatc aaaacattcc ctttgatggc tggagaagag agcaacacat
     5221 atacttacaa agatgaacac ggagattata acttgatcga gatagatggc gatcgagtac
     5281 ggataaaaga agcagattgc gatgaccaga tctgcgtacg tcgaggctgg gcaacgaaaa
     5341 atggcgaaac catcgtctgt ttgccacata aattagtgat cgaagtccgt tcaacagatg
     5401 gaggtgacga agacagtctg atttattaga ccttcacaca ccatgagtaa attacaaaaa
     5461 atgatttata tttcgctact tgttgcacaa ggagtgatca ttggtttatt ggaaaatatg
     5521 atcccttatc ctttttcttt tgcgcctggt gcaaaactag gcttagccaa tctaatcacg
     5581 atcgttgcca tttttaccat gaaaaaacaa gacagcttct tactactttg gctacgacta
     5641 ttcctgacta ctttgctagg cggaacgatt tccacattcc tttatagtat gagtggtgct
     5701 ttgcttagtt attttggaat gttattggtc aagcaattag gaccaaaacg tgtcagtatc
     5761 atcggaatta gtgcggccgg cggtttcatg cataatgtcg gacagctttt gatggcttct
     5821 ttcattgcac aatcatggac agtcatgttg tacttgccag tcctttcagt tttagggatt
     5881 ttatcaggga ttgcaatcgg gatcgcagcg aattatcttt tacgacatgt gcagacactt
     5941 caacgattcc aaaaagatta tgagcaggga accaaacaaa aatggagccc aatgtttacc
     6001 aaaaaataat cgtcacacaa ggagtttttt tatgaagcta catccaatgt gggaaaacta
     6061 tcctgcttta agtaaagaat tgacagaaac actaaaacgg atggaagaaa ctgtccgcct
     6121 gaaaaacaaa cctgtcgaac aagccatcaa agaaacgatc catgccggcg gaaagctgtt
     6181 gcgtcctgcc tatcagctat tgttctcaca atttggacca gagcaagatc gagacaaagc
     6241 catcgctttg gcagcttcta tcgaaatgct ccatacggcc acactgatcc atgatgatat
     6301 cgttgatgaa gcggatctgc gacgtggtca gcctaatatc cgtagtcagt ttggtaatgt
     6361 cgtttctgtc tatgcaggag actatttatt tgtctgctgt tttaaactat tgtctgatta
     6421 ctccacttcc ttgaaaagtc tccaattgaa ttctcgcagt atggaaaaag tattgaatgg
     6481 cgaattaggg cagatggatg atcgctataa ttatgaactt tctgtaaaag aatatttgga
     6541 aaatatttca ggtaagacag ctgaactgtt ccaattaagt tgttctgttg gcgcttttga
     6601 aagtggaaca agcgagcgct tcgcaaaaaa agcaggagat atcggacttt ctatcgggat
     6661 ggcctttcag attatcgatg atattttaga ttatacaaaa gagagccagg aaatcggcaa
     6721 acctgtacta gaagacatgc gtcaaggggt ctattcttta ccgttgatct actctttgca
     6781 aaaaaacaaa ccaaagctac taccttattt agagaagaaa gcggcaatga cagaagaaga
     6841 tgtcgacgcc gtacgcaaaa tcgttgaaca tacaggaggc gtggaagaag ctaggaagtt
     6901 agcggcctct tacacagaaa aggctttgaa ggaaataaag aaattacctg ccacctctct
     6961 tcgaacaaaa gagaatctct tttcccttac ccaactgatt ttggatcgca aagactgaat
     7021 agggaaaggt tcgatacgta aataaatttc tcagaaggaa atctacgtgt tttttgacaa
     7081 attgaattgt agttcttgta tttatgataa aaagcaaaaa taaagagcaa cgtccgttag
     7141 cgacgttgct cttttttatc ttgaatattt ttttaccttt tcgatttctt ataactcata
     7201 aaagtgattc ttaaaccgat tacaagtaat ataacgcctg cgagtgttat ccaattattc
     7261 gattgacttc cagttttagg aagtgctctt tttgatttgt ttccattgtc attgcttgtt
     7321 ttattagtta agttaaagtt cgtcctgtta tccgaacttg tttttgagaa ggagacactt
     7381 gcatgacgtg tatcgcttgt ggtagacgac gtgtctgtac tatcgcttga agttgatgaa
     7441 tctgagctat cacttgtggt agaggatgta tctgtactgt cacttgaagc tgacgtgtcc
     7501 gtgctatcac ttgtagtggt agatgaatct gagctatcac ttgtggtaga ggtagtatct
     7561 gtactgtcac ttgaagctga cgtgtccgtg ctatcacttg ttgtggtaga tgtatccgta
     7621 ctgtcgcttg aagctgacgt gtccgtgct
//
LOCUS       DARBFO010000109         7601 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000109 DARBFO010000000
VERSION     DARBFO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.112"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(943..1356)
                     /locus_tag="SCK51_002558"
     CDS             complement(943..1356)
                     /locus_tag="SCK51_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEG1253127.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="SCK51_002559"
     CDS             complement(1812..2297)
                     /locus_tag="SCK51_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253128.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="SCK51_002560"
     CDS             complement(2436..2651)
                     /locus_tag="SCK51_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEG1253129.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="SCK51_002561"
     CDS             2875..3675
                     /locus_tag="SCK51_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1253130.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="SCK51_002562"
     CDS             3694..5562
                     /locus_tag="SCK51_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEG1253131.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="SCK51_002563"
     CDS             5555..6046
                     /locus_tag="SCK51_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEG1253132.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="SCK51_002564"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="SCK51_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEG1253133.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..7601
                     /locus_tag="SCK51_002565"
     CDS             6606..7601
                     /locus_tag="SCK51_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEG1253134.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2709 a         1213 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg a
//
LOCUS       DARBFO010000110         7551 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000110 DARBFO010000000
VERSION     DARBFO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.113"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            135..2606
                     /locus_tag="SCK51_002566"
     CDS             135..2606
                     /locus_tag="SCK51_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1253135.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2709..4445)
                     /locus_tag="SCK51_002567"
     CDS             complement(2709..4445)
                     /locus_tag="SCK51_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEG1253136.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4442..5146)
                     /locus_tag="SCK51_002568"
     CDS             complement(4442..5146)
                     /locus_tag="SCK51_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1253137.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5277..5780
                     /locus_tag="SCK51_002569"
     CDS             5277..5780
                     /locus_tag="SCK51_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEG1253138.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5740..6078
                     /locus_tag="SCK51_002570"
     CDS             5740..6078
                     /locus_tag="SCK51_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEG1253139.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6099..7517
                     /gene="ffh"
                     /locus_tag="SCK51_002571"
     CDS             6099..7517
                     /gene="ffh"
                     /locus_tag="SCK51_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEG1253140.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
BASE COUNT         2420 a         1396 c         1601 g         2134 t
ORIGIN      
        1 cctttttttt tgctatatga caggaatgaa aggtgtttgc aagcttaatg tgaggaacac
       61 tttccatatc tgttctatac tggataggag aagtttggaa atatccagaa aacgaaatca
      121 atcgaggtag ccacatgaag aataaatgga taaatcgaag taaaaaaaag aaagtaacac
      181 aaagatcatc agataaagtc atccgtcttt ttccatcgta taaagaagga ttaacggcag
      241 atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat cccacattca
      301 aaacaaatca gcaaatcgta ttggaaaaca tatttacgta tttcaatttg atctttctta
      361 tcttagccat tttgctttgt ttagtagaat cctataagaa cttgactttt ttaccagtta
      421 tcatagccaa tacaggaatc gccatttttc aggaaattcg atccaaaaag atcttggacg
      481 aactaaatgt tttaaataca gcaaaagtca atgtcatacg agacgggata gaacaaacaa
      541 tagatataga agaccttgta atagatgaca ttgttatttt agagactgga tatcaaattc
      601 cagcagatgc agaggtcgtg gaagggaaac tgcaagttaa tgaggcttta ttgacaggcg
      661 aagcagatga aataaccaaa gaaataggtg atccattaat gtccggcagt ttcattgttt
      721 ccggaaaagc ttacgcccga ttagacaaag ttggagcaga ttcgtatatt tcccagctga
      781 cagtcaaagc caagacaatg ggagaaggag aacagtcgga gatgattgct tctcttaatc
      841 aattgatcaa atggattggg atcatcatta ttcctatcgg tatcctgctt ttttctcaaa
      901 gttacttttt taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa gcagcattga
      961 tcggtatgat acctgaagga ctgtacttgc tgacgacgat tgcattagca ttaagtgcga
     1021 ctaaactagc aaagcaaaga gttcttttac acaatatgaa aagcattgag acattggcaa
     1081 gggtaaatgt cctttgtgta gataaaacag gaacgatcac agaaaataaa atgtctgttc
     1141 aaaaaatgat cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa ttggaagcat
     1201 gcattgctga ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa gctgttaaag
     1261 catattttac aaaaaccact gatcaatcct atcaccatgt tattcctttt tcttctgtcc
     1321 aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg cccgaaatgg
     1381 ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt ttctttcgct gaaaaaggct
     1441 atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga agaaccagta ttgacagaag
     1501 cagtcacacc attaggctat attctgattg ctaatcctat cagaaaagaa gcaagagcca
     1561 catttgatta cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga gataatccgc
     1621 tcaccgtatc caatgtagct gtccaagcag ggatttcaca tgctgatcag tacgttgatg
     1681 catctaaatt atcagaagaa gaatatgaag cagctgtcga aaaatataca gtttttggac
     1741 gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgat aaagcgtaac aatacagtag
     1801 ctatgacagg tgatggcgtg aatgatattc tagccatgaa agaagcagac tgcagtatag
     1861 cgatggcttc cggaaatgaa gccgccatgc aggcctcaca agtggtctta cttgattctg
     1921 acttttcacg catgccagaa gttgttgcag aaggacggcg cgttgtgaac aatattgagc
     1981 gatcagccag cttgttttta gtgaagaata ttttctcctt tttgctctca ctgttttcag
     2041 tgatctttgc tctgacctat cctttagaac catcacaaat cacattgatt agtttgttta
     2101 ctatcggact tccatctttc ttgcttgctc tagaagaaaa caaaaagcga atcagaggga
     2161 aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg actaacagat atgatcgtag
     2221 tcggtgcgtt ggtgatctgc ggggtgactt tgaatctaaa taaaacagat gtttctacag
     2281 catctaccat gcttttgatt gcagtagggt tcctcgttct ctataaaatc tgttcaccgc
     2341 tgaataaatt cagaagtcag atcattcttt tctgtgctag cggtaacttt ttttcggtca
     2401 tctttttgca taagctgttt tcaatcaccg gtatctcagc tgtatcactt ttgctgttga
     2461 gcattctatt tttctcggca gattcgattt ttaggcattt gaccacattg gtagaatatt
     2521 tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag tcgcgtagct aaaaaatttc
     2581 tatcgatatt taaacgagaa aattgacgaa aagtatctca aaaaagagta aaaaagaaca
     2641 accactcgtg tatctttcga agcaggatga gtcatccaac tttaaaacgc acgagcagtt
     2701 gctctttttt acattttcgg aatggtaacg gtgaaagtcg aacctgttcc aagatgactg
     2761 tctacagtaa tcgtaccatt aaggagttgc acgtagtctt ttacaatcgc taaccctaat
     2821 ccggtcccgc ctgaatgtcg gcttcgcgcc ttgtctacgc ggtaaaaacg ctcaaagatc
     2881 cgttcctgat cctcaatgtc gatacctata ccaaagtctt ggacagaaaa cgatagatca
     2941 tctgttgcct gatagcggat aataatctcg ctgtcgcttt ttgaatattg gacagcattt
     3001 tcaattagat ttttgataat tggataaagg atatctgttt tagtagtaaa tggttcgttc
     3061 tttccaatca atcgaatcgt caaccgtttc ttttcgatga ttggctgata attttgaacg
     3121 atttgctgga aataaggttc tgtataaatt gtctggatct cgtaagaatt ctcgccatct
     3181 tttgacagct ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc tttttgcatg
     3241 atctcaagaa aagaagtcag agtttgcggg tcatcttttg caccatccag taaagtctcg
     3301 gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt gagagacgtt gccaacgaaa
     3361 tccttttgta gcttttctaa acgtcgcact ttcgttaagt catacgccac ccctagaatc
     3421 tgtccggtac cttctgaatt agtaaagtag cgcaaactca agtctaatgt ctgccgacct
     3481 tctgtaaggg tgatctcctg atggatcagc ggatgctcag gagtcacttg atgaatcagc
     3541 tgaatgagtt ttggctcttg aatgacttct gtgtaccgct gttcaggttg atagcttacc
     3601 acgtttaaat gtagctgcat ctttggatta agcagtagta aacgagaatc agcatcatca
     3661 atcaggaata cgccaatcat cagttcgttc agtaacgtat ataattgctc ttctgtggta
     3721 gtacacgccc gatacatctt actcatttgt tcgctcaacg cattaatcgt ctgatacaat
     3781 tcttcccatt gatcgggtgt ttgcatgatt acttccgcct gattaggatt ttgcaccatt
     3841 ttttttagta cgggcagcac ggttttcaat ggctggtttt tttgatagat cagataatag
     3901 atcattcctg caagaacaag gaagaaagac agaaagaaga aaaagaccca acggcgaaaa
     3961 ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga tacgtaaaat ggcttctaac
     4021 tggccacttt ttttgactgg taatgcgaca tataaaagat cctcattcaa agtagtactt
     4081 ttgcgcaaag cgctacctag gttcccacca ttcaatacgg ctttgatttc gggacgggag
     4141 tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata agatctttcc agtagcatcc
     4201 attaaagtaa tccgttcgtt cgactgatgg acgaattctt ccaaagcagt tttgttttgc
     4261 gctgcttgga ggttttcgac atctaattgc cggatcagca gttgtgcttt ttttgtcagg
     4321 tagctttctt gctgttccac gatctgctgt tgaaaataat gggaaatcaa ttgccagcca
     4381 ccaaaaaaca aactaactaa aagaataaga aagaagagac gttcataaat ttttttccat
     4441 ttcatcgttt gggttcctga aacttatagc caaatcctct aatagtcacc aagtactttg
     4501 ggtgttttgg atctcgctcg attttttctc gcaaatgact aacgtgtaca tctacgattc
     4561 ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag caatgtgtct cggtcgatca
     4621 cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt gttagttcaa
     4681 tcagatggtc gtctacggtc acttgataat tggtcaaatc agcagtgatt tgtccaatcg
     4741 atagatattc aggctcagct tcatcttttg attgcttacg aggttcaatt cgtctgaata
     4801 ttgctttcat acgggctaat acttcgcgag gactaaatgg tttggtcaaa tagtcgtcag
     4861 cacctatttc taggccaatg atccgatcca cttggtcatc tttagccgtc agcatcaaaa
     4921 tcggtgtttc atttttttct tgacgcagcc gctgagtgat ctccatgcca tccatcgttg
     4981 gaagcatcac atctaaaatg atgaaatcaa atgattgttc taatgctaat tctaatcctt
     5041 cgcctccatc tgtggcactg gttaccttgt agccttcttt ttccaaattg aacgtcaaga
     5101 gcgttacaat agaaggttca tcatcaacaa ctagtatttt tttcattcaa atctccttta
     5161 aataggtttc attctgttta tattttagca tagaatcagt acaaagggct attttacaaa
     5221 agaaataaat ggtataatag gaaggcactt tgctgaatag aaaggaagag cagtcaatga
     5281 ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta tgatggacag aaaaaagaag
     5341 taccagaatt gatggaacta gttgaaactg gagaagctgt catggtctgt ccagaagtga
     5401 tcggcggatt accaattcct agagatcccg ctgaaatcat tggtggaaat ggattcgatg
     5461 tgtggaaaga tcaagcaaaa gtatggacga caagcggcga agatgtaacg gaagcgtaca
     5521 aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat ctagtcatca
     5581 tgaaggaaaa aagcccttca tgcggaaccc attcgattta tgacggaagt ttttccggaa
     5641 taaaaaaaaa tggtgccgga gtggctactg cttattttat tcagcaaaaa atgactgtgc
     5701 tctctgatga agaatggctg aaaactagag gtgagcagaa tggaaatcga aaagaccaat
     5761 cgaatgaacg cgctctttga attttattcg accttattga cagaaaaaca aatgaactac
     5821 atggaattat attatgcaga tgatttttca ttaggtgaga tcgcggaaga atacgaagta
     5881 agccgacaag cagtatatga caatatcaag cggacaagca agatcttgga ggattatgaa
     5941 aaaaaactcc atcttttttc taattatatt gttcgagaac aggtattgga aaatatgact
     6001 gcatatatta cagataagta cccggaagat aaaaaactac ttgaatatgt acagcaaatt
     6061 caagcaatcg aagaataaat aaaaggaatg aagaaaatat ggcatttgaa agcttaactg
     6121 aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca gaagctgacg
     6181 taaaagaaat gatgcgggaa atccgccttg ccttactaga agccgatgtt aacttgcaag
     6241 tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa gtgctggaaa
     6301 gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg atcacacttg
     6361 gttctgaaac agcagagtta aataaatcac caaaaatacc aactgttatc atgatggctg
     6421 gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt agccaattat ttaaagaaaa
     6481 atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata tcgaccagct gcgatcgacc
     6541 aattgaaagt attaggtcaa cagctagacg ttcctgtatt tgatatggga actgaagtca
     6601 gcccggtaga gatcgtccgt caagggatgg agttagcaaa agaaaagaaa aacgactatg
     6661 tattgatcga tacggcagga cgtctgcata tcgatgaaac attgatggat gagttgaaac
     6721 aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc atgactggtc
     6781 aagatgccgt caacgtagct gatagcttca accagcaatt aggtattaca ggggtcgtca
     6841 tcactaaact tgatggggat actcgaggcg gggcagcact atcgatccgc tcagtgacag
     6901 gcgcaccaat caaattcatc ggttctggtg aaaaattgac tgacttagaa gtattccacc
     6961 cagatcgtat ggctagccgt atccttggta tgggggatat gctgacattg attgaaaaag
     7021 cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg cgggaaaatt
     7081 cattcgattt caatgacttt attgaacagc ttgatcaagt gatgggtatg ggcccactgg
     7141 aagatctaat caaaatgatc ccaggaatga atcaagtccc tggaatcgaa aacgtcaagg
     7201 tagatccaaa agatgttgaa cgcaaaaaag cgatggttta ttctatgacg ccagcagaaa
     7261 gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc ggttctggaa
     7321 acagtgttgt tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga aaaatgatgc
     7381 agcaaatgtc caaaggggat atgaacatcc caggtatgga ccaaatgttt ggttcaggcg
     7441 tcaaaggaaa actaggcaaa atggcgatga atcgaatgat caagaaaaat aagaagaaaa
     7501 agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac agactgaaaa t
//
LOCUS       DARBFO010000111         7548 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000111 DARBFO010000000
VERSION     DARBFO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.114"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..715
                     /locus_tag="SCK51_002572"
     CDS             <1..715
                     /locus_tag="SCK51_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEG1253141.1"
                     /translation="NIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVT
                     GIVNSAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNAL
                     KDTIKGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNL
                     VKTPSVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTE
                     LTRQMKRDAGAVGGTLYAT"
     gene            705..1445
                     /locus_tag="SCK51_002573"
     CDS             705..1445
                     /locus_tag="SCK51_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1253142.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1442..4204
                     /locus_tag="SCK51_002574"
     CDS             1442..4204
                     /locus_tag="SCK51_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1253143.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            4217..5125
                     /locus_tag="SCK51_002575"
     CDS             4217..5125
                     /locus_tag="SCK51_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HEG1253144.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            5125..5745
                     /locus_tag="SCK51_002576"
     CDS             5125..5745
                     /locus_tag="SCK51_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253145.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSRTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            5749..6198
                     /locus_tag="SCK51_002577"
     CDS             5749..6198
                     /locus_tag="SCK51_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253146.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKAKLDGLANITFEKVGTV"
     gene            6198..6692
                     /locus_tag="SCK51_002578"
     CDS             6198..6692
                     /locus_tag="SCK51_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253147.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            6706..7137
                     /locus_tag="SCK51_002579"
     CDS             6706..7137
                     /locus_tag="SCK51_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253148.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            7139..7276
                     /locus_tag="SCK51_002580"
     CDS             7139..7276
                     /locus_tag="SCK51_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEG1253149.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEEEADKIISKEESA
                     S"
     gene            7314..>7548
                     /locus_tag="SCK51_002581"
     CDS             7314..>7548
                     /locus_tag="SCK51_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253150.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHT"
BASE COUNT         2730 a         1037 c         1595 g         2186 t
ORIGIN      
        1 taatattaaa cagtttttca aagatacttg gaaaaatatt aaagatacgg catacaatac
       61 gtggatttct ataaaaaata ccatgattaa tacatggaat aacattaagg actctttctg
      121 gaatattgtt actggaattg ttaattctgc tgaaaatgca tggacaaatc taaaaaatgg
      181 tgtttcaaat gctattaatc gggtaaaaga aatctttaat tcgttaaggg aagtcaattt
      241 atttgaaatt ggcaagaaca tcattgatgg acttataaat ggtgtaaaag aaaagtggaa
      301 tgctttgaaa gatactatca aaggtattgc aggtaatatc aaaaattgga ttaaaggagc
      361 tttagatatt cattctccat caagatggat gcgtgatatg gttggtaaaa atattgttca
      421 aggtattatc gttggtattg ataaagagca aagtaagtta gatcaaacca tgacaaattt
      481 agtaaaaaca ccgtctgttc aacctgtagt aaatggaata agtcacctac caattacaca
      541 aacaaagcaa aatatatctg ctagtgaaac aaaagaaatt catttacatt taaatgttta
      601 tggagattta cctgattcaa tggttcggaa gattaccaca aaaattaaaa cagaattgac
      661 aagacaaatg aaacgagatg ctggtgcagt aggaggaaca ttatatgcaa cttaagagag
      721 gacaattttt tattaatcaa cattattctt ctgaatttaa tgtgtatatt caaaacaggc
      781 ctgcttctgt ttcagctagt cgagtaattg aattgagaga gcgagaagga aatgattcta
      841 tcattattga taaaggctat tacaaaaatg tgactagaaa gattgagtgt tactataaag
      901 caccttcaat tgatatggtg caggagtggg aagatcgaat cactgaatgg ttagatatgt
      961 cgtcttatag tgattttatt ctttactatg atgaacaata tatttatcaa gctgtagtaa
     1021 tagaaggacc tgaatttaaa ggaacaagaa aaacaggaaa tattgtccct tttgaattta
     1081 cagtgagtat tcgaccattt aaggaaaatt acaatggtag atttactatt cgacaaacag
     1141 aaacttttga aatttataac ccagagaagt attcttcaaa accgcttatt aaattgagtg
     1201 gttctggaga tgcttctttt tatattaata aggataaata tgatttgaaa tcgttggaca
     1261 gagaattatt tatagattct aaattagaag aggcttatcg aaaattagat ggtaatttag
     1321 aacatcagga tcaggtcacc ttatttttag attttccatt tttatatcct ggaaaaaatg
     1381 aaattaaatg gacgaaaaat attcattcat ttgaaataat gcctaggtgg tggagaaaag
     1441 tatgaaacca agaatatata gtcctactga aacagatttc tcaacgaatg gcttaggaat
     1501 tttaaaggat tgtacaagat gtggaatata tgaagtagct aatggaaaat atgaattaga
     1561 gttggattat cctttaggaa ctagatttga tgaatatttt gaaaatgact atcaaataaa
     1621 agcaaagcca aacgatcaag aagagtatca tatctttttt attgatgata aagatataga
     1681 tactttttta aatacagtaa ctatttatgc tcagagtcgt acaaatcgac ttggaagacg
     1741 ggtagtcact cttgtggaag tggactctaa aactggtcaa gaagcgatat caattattga
     1801 aactaaaatg gataaaaaat ctgacatacg actttattct gatattacgg ctgtttctag
     1861 cacaatcttt gaagcgagaa atgttttaaa ttgtattgct ggtgaacaag gatcattgct
     1921 tcaatattgg ggcggagaaa ttaaacgtga accatttaag ctttctttgt taaagcgaag
     1981 aggtcgcaat aatgttggaa cgattcggta tggaaaagat atgtctggtt tgaaggtcaa
     2041 attagattgg acaggtataa aaacaagaat tattccatat gctgatcctc aaagtgaagt
     2101 aggtacgact aatcgaattt atggttcgcc agtagatagt gcctatatta ataactatcc
     2161 tgatgtctat acagaacatg ttcagtttac agaagaacaa ggagtaaaag acattaagag
     2221 cttaaataaa atagctaaaa attactttaa aacgatcaat cctggctgtg ataagcctaa
     2281 agtttctatt acggttgaat ttgataagtt gactgatagt gaagaagcga aagaatttgc
     2341 gaagattaga aattatggtc tgtttgatac atttaaaata tatcataaaa aatataatat
     2401 ttatcttgaa tcaaaggtta gtggactaca atacgattct ttatctgaaa aaactttgaa
     2461 attagaagca ggagacattc aagttgcttt ttatcagcaa caagctgtaa ctattcagga
     2521 taaattaaaa gattatgcaa cgaataatta tatgagtgat tttaatgatt atgtttcttc
     2581 aatgattaca ggtcaaggca gtgcaggagg atatgtagtt ttatggccaa aagaaaaacc
     2641 ttccaacatc tttattatgg ataatccaga tttagaaaag gcaagagaag tactaagaat
     2701 gaataaaaat gggattgctt tttctaagaa aggatggcgg gggcctttta attcagcttg
     2761 gacattagat agtatattta atgccaattt tatacaaact ggattaatca aagcagatat
     2821 ctttcaaaat tcttttaaca agactggaga tgtattgaaa ttagttaatg gactactcca
     2881 aatttggaac aacaaaaaga aaataatgga gttaactaaa aaaggaatgg aattttggaa
     2941 ttctaagggt tcaattggaa cgattggaac aactgattct gttggtaatc cttttcctgg
     3001 ggcttctact ccaaccccta ttgaagataa ttctttagtt attcgtacaa atggagacgg
     3061 caaatatatt ttgatttctc ctaaagtagg aaaaggatta gttttattag gaaatggtaa
     3121 agcaatttat tttggagact tagatgtaca aggaaaactt acagttaaag gaaaagaaat
     3181 aattcctgga caaggtggtg gtccatcggg aggtggagaa tctactggtg ggtatcctag
     3241 tgaattaaaa acagatgcag aaaagagagc ttggagaatt tacgatattt tgtgtaataa
     3301 cggatttacg aaacaatctg catgtggaat attggggaac attcaacaag aaacaggagg
     3361 aacttttgat cctgatactg ttcaaatagg tggaccagca tacggattag ttcagtggga
     3421 tggttcttca tatcctcttg ttggtccagc aacatgggat ggaaaagttt atgttcaaaa
     3481 cttatttaat gctgcaggta ttaaagaacc aataacgagc ttagatgcac aagttcgatt
     3541 gcttatttgg acatttacaa atggacaatg gatgggcgta gtacaaccta cgacggttga
     3601 tggatttaag gcctgtactg atccaagaca agcagcatat gcttttgaac gaaactatga
     3661 acgtccggca gcgacacatc ctgaacgtca agattatgca gttaactggt ataacaaatt
     3721 taaagattta aaaccaggag gagctactgg agaagcagga ctaaaacatt tagagtcttt
     3781 gattggacaa cgaattggca atggtcagtg ttatggcttg tctgcagaat attcaggata
     3841 tctgggtggt tgtggcatgg gtgctggaac aaaatatggt ttaactcatg tgataggaaa
     3901 tacttctgca gcatctgata ttggtattgc ctatgattgg tctgctgttg gttggaaagt
     3961 gattcaaaac cctagatatg atcaattagt agttggtgca attattaatt gggcaagagg
     4021 tggacaagtg ggttcatggt ttgcagatgg aacttatgga catactggcg ttatccgagg
     4081 cttagctaat ggtcgtatgc aaacttatga acaaaacaca gagttaggta tgatttgtgg
     4141 aaagttagat cgccagtatt atagtgcaag tgcaatttct tccattgtca taccaccaaa
     4201 atagggaggt gatttaatgg caaagtggaa tgtcatatta agtacaacag aaccctataa
     4261 ttacgtgggg atgattcaag ttcgacaagg caataagaat acagaggtta tggaagcgac
     4321 tatagttgaa aatggtcttc cctacgattt atcagaatgt aaggtatatt ttgaatcagt
     4381 tgtaggtggg aaatatccag tccaattaga aacaaaaatt gtggatgcta aaaaagggaa
     4441 aattaactat atttttgata aatattccat gcagtgttta catcgacaaa cagccaattt
     4501 cattatattt aaaggagaag acttgattgg aacaactcaa gacttctctt attttgtcat
     4561 taatgctgtt tcaaaaacag aaggagaaat gggttcttat tggcaatcaa tcgaagattt
     4621 aattgcggat atgacggact ttattaatga aaataaaggc gattttacgg actggatgaa
     4681 tgaaaggaaa gaagaatttg aacgttggag acaagaacag gaacacagtt tccaagattg
     4741 gagagaagga caagaatcag actatttggc atggtttgaa tcaatcaagg atattttaaa
     4801 gacgattgat ccaggtggag tcatgttagc cgaactaacg gatgcgcgag tagatattca
     4861 aggagttcgt catgcgtcta tttcagagcg tttgctggca gatatggact atttatatca
     4921 gaaattgcga gcaacgcttt tcacgattga atacggggag attgaagtga ctgatatttt
     4981 gcaggatgat ctcttttcag ataatcatga agtcgaaaaa gttggaactg tagcattccc
     5041 gattgaagaa ggagccttga tcatcgcaac cgttgatgat ccaaagcaaa atgtgttcac
     5101 tcttgagaaa gttggggtga tctaatggct aaaactaaac gaatgatgga aacggatgaa
     5161 aaaacgggtg tacaacgcca attctttcca atcacacatg cttccgcagt tcttgggtta
     5221 gaagaaataa tagcaggaga agcaacagtt ttatctgtta atggaaaaat tggagccgtc
     5281 gtcattacta aagaagattt gggattagag aatgttctca cagaattacc ctatgcaagt
     5341 gaagaagatg acggcattat cacggcagaa atgtatcaaa aaattttaaa cagtggagaa
     5401 ggtgactacg tgttgccagt tgccactatc gaccgtttgg gtggcataaa agttggtgaa
     5461 ttattgacga ttgatgagac agggaaagtc tctgcagtca gacaatctga tgtcaatttt
     5521 tcgctggagt taaaagaaaa actcgattca ctgaaaaatt atactgctgg agaaaacatc
     5581 actattgatg aagatggaac aattagttct acaggtggta gtaggactgg tggtgtcaat
     5641 cagtcatatg tagatcaaaa attccaagaa gctgtaaatc aagcagaaaa ttacacaaac
     5701 gaaagaattc caaattttac ttttgaaaaa attggggagg tatagagaat gacagatatc
     5761 gtaaaaataa aacaaagcaa tgtacaggtt tatccacaaa ctcattggaa tgctatagaa
     5821 ggcaaaccaa caacggtgaa gggagataaa ggcgatcctg gccaagcagc aacaattacc
     5881 ataggaaccg tttctagtgg ttcaaccgct tctgttacga atgtaggaac atcatctgca
     5941 gctagattta attttgtttt accaaaggga gataaaggtg atcctggaat aaatgccaca
     6001 actacagctg tagctacaac tacagcaaat gggttgatgt cgtccacaga taaaactaag
     6061 ttggatggaa ttgcagctgg agcacagaaa aatccaggta atgctacaac tacgacagca
     6121 ggtttaatgt cagcaactga caaagctaaa ctcgatggat tagccaatat tacatttgag
     6181 aaagtgggga cggtttaatg gctgatattg tgcaattaaa agaagacgga gttgctaaat
     6241 acttaaaaac gcatgtagaa gctattgatg gtaaagaagc attggtgcag acagatggag
     6301 accaagccat tgcaggacat aagaattttt caggttctgt aacaatcaat aataaacgtg
     6361 ttttgacgac tgatgataac agatatgaag tggtaaatct ggttgtcact aacggtaata
     6421 cagggacagc aaagctttat cgtgaaggaa aaacagtttc tatatatttt gtggctttaa
     6481 acggaaaaag tagtggcggg aatgattcgg ttattttaac tgttccagaa ggctatcgga
     6541 caccaattag ttttgaacaa ctggttgggt caatagaccg ttctactttg aacagtgctc
     6601 aactatctat tggcgcagac ggagccatta aatggagaag aaattcaagt tatggatcag
     6661 cttattcatt tgttatcact tattcaattt aagggaggaa atctaatgaa agtagtttac
     6721 aaatcaatca agccttacgg attcgagcaa atcattttga acaatcaaga aaatatccct
     6781 gaaaactgta cagagattaa accaccagtt cctaactgga gaccaagatt tgattttgat
     6841 aaaaaacagt gggttgagtt agctactgaa gaagagaaaa aagggaatgc tgttgatagc
     6901 gtagatgaat tggctaatat taaggcatta tacgaaacgc tgaaggcaga aaatgatgaa
     6961 ttgaaacagc taaattctaa agcaatgcta aacaatgtag caatcaagca agaaaatgtc
     7021 ttattgaaag aaaagtcaga aagtttagcg cagttgaatt caaaaacgat gcttgcttct
     7081 gtacaaaata ccaaggaaat cgaagaaatc aaaaaacaat tacaaggtgg gaagtaacat
     7141 gtattcatat gaagatatca aactaatgta tgactggggc tgtttcacta acgaacaagt
     7201 aatggttttc gttccgttgt gcattactga agaagaagca gataaaatca ttagcaaaga
     7261 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     7321 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     7381 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     7441 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     7501 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatacag
//
LOCUS       DARBFO010000112         7359 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000112 DARBFO010000000
VERSION     DARBFO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.115"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..692
                     /locus_tag="SCK51_002582"
     CDS             <1..692
                     /locus_tag="SCK51_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253151.1"
                     /translation="CIFVLLLHGRVINLVNRKSTTKFANSFWFLKNINKTVEKNGVYK
                     GDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETYSQVLKRIMSQETSFIYELSK
                     SNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRILKSLFDLKLAKATIMGIERD
                     SYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLKCRGLDFIGTLGWTETYYEKI
                     YKEIIEQYFPK"
     gene            875..3550
                     /locus_tag="SCK51_002583"
     CDS             875..3550
                     /locus_tag="SCK51_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HEG1253152.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3732..3971
                     /locus_tag="SCK51_002584"
     CDS             3732..3971
                     /locus_tag="SCK51_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253153.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3987..5360
                     /locus_tag="SCK51_002585"
     CDS             3987..5360
                     /locus_tag="SCK51_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1253154.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            5364..6998
                     /locus_tag="SCK51_002586"
     CDS             5364..6998
                     /locus_tag="SCK51_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1253155.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            7161..>7359
                     /locus_tag="SCK51_002587"
     CDS             7161..>7359
                     /locus_tag="SCK51_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253156.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2625 a         1142 c         1458 g         2134 t
ORIGIN      
        1 tatgtatttt tgtcttatta ttacatggaa gagttattaa cttagttaat cgaaaaagta
       61 cgactaaatt tgcaaactca ttctggtttt taaaaaatat taataaaaca gtggaaaaga
      121 atggagttta taaaggtgat tttcattttc aaaatgctga gactattgaa cgagaacatc
      181 attatttcga tttattagtg aatacagtgg tagaattagc gaaatgggca aagtcaaacg
      241 aaacttatag tcaagtactt aaaagaatta tgagtcaaga aacttcattt atctatgagt
      301 taagcaagtc aaatttgaaa tatacttata atgaagatga acttgaaatt ttagataatc
      361 cagaaaattc acaatggatt aaagagttga agagagaaga ctttattgag acactaagaa
      421 ttctaaaatc gttatttgat ttgaaattag ccaaagctac catcatggga attgaaagag
      481 attcatatta ttgggaggaa attggactat atgctcccca gattgaaggt gaagataaag
      541 agaaaaggat agacaacatt attaactata ttaatagttt aagtgaacct ttaaaatgta
      601 gaggacttga ttttatagga acattaggat ggacagaaac atactatgag aaaatatata
      661 aagaaataat tgaacaatat tttcctaaat aatttaataa ggattgattt atcgtaaaag
      721 aaaaaagttg tgatttccaa aatcacaact tttttctttt gtcaagattt tgagtgggat
      781 atatttttgt agaactgaag atcatgaaat tatgagaggt tggaggtaat gtagtcatac
      841 aaataaaaat tttttagaaa gtgagtggta attaatgaaa atgaatcaca cttcaattca
      901 gaagcattta caactgttaa atacactggg gatttcttat gttaatttga gaactttttc
      961 tacattaaac aagaaaaccg tttctcatat tttttctact gcagattctc aagcaattat
     1021 taactattta gaaaaacaga aattgtctga aggtacagta aacataactt ataatcctat
     1081 taaacaagag gtgattacac aaaaaaagca ttctgtgaga gacaatgata ttgaaaaaat
     1141 tcggtttgta tttattgata ttgaccctaa aaggaaagag ggaagtgcaa cagatagcga
     1201 aaagaaaaag gcagaaaatg ttatggaaca agtagaacgt tatttgaaag aaaagagaat
     1261 agaatcattt gtaaaagttg atagtgggaa tggctaccat atttttcttc ccataaatga
     1321 acaaccaaat aatcacgaga cgattcttac tattcagaat tttcttcaat tacttcaccg
     1381 aagatttgga gtagaagatg aagttgatat tgatacttct gtttataatc cttcaagatt
     1441 atgcaaatta gttggtacac cagcggtcaa aggaaagtcc acaaaagagc gtccgcatcg
     1501 tttatctagt ttcctttcta ttcccgataa catagataga agtaattgtt ttaaaattct
     1561 tgaacagata gttgaagaaa attccatgat gtcaacctct agagtaaaaa gccatccatc
     1621 ttttagagaa gtgttacctt ttattcaagc agactataaa caatggttag ctcactatcc
     1681 tgaattaact tataccataa aagagggcga tcataaagga gttaccttac ttatatttga
     1741 cgaatgtcca ttaagaaaac acacgaataa ttctaatggt tcttgtttgt ctgttacggc
     1801 tgataataag attcgatttc attgtctgca tgcttcggat aaaaataaag atattcaaga
     1861 ctttgtaaaa aaatatccgt tgccaaaacc tgtaaatcct tctgtagaat cattagaatc
     1921 caatcaagaa tattattttg accagtttaa actgaatcaa gaaggagtat ttttggtgaa
     1981 agaaggtcaa gaagaaaaag ttgcttgtcc gatgtttatt aagcaaatac gcaaaaatat
     2041 cgaacaaaaa gtcattacac ttgttcttgc ctattatgtc gatcattgtt gggatgaaat
     2101 agaaatagaa ggtggatgtt tacaaactaa taattttaaa caattagcaa aacatggctt
     2161 aatcattaaa gctagaagag aagctgaagt cgttgacttt ttacagattc aacgggagaa
     2221 tgctccaatc ttttttgaac atgaatctct tggctggaaa aaagaaaatg aagcaatgat
     2281 ttttcagtta caagattctt ttggacgtga aattccttct gtattaacta aaaactcatg
     2341 ctaccaatta acaactaatg gtacagaagc ggaatttgac gcattggttc ataagtatgt
     2401 tttaggaacg aacttagaac ttgcttggtc gattggtatt gcttctattg tgttaggcta
     2461 tttaaacgtt acgaaggcta atactttttt gagtttacta attaacctac agggcaagag
     2521 cacaacgggt aaaaccacta cgttacactt tattgcctct ttatttggaa atccaactac
     2581 ggttcttaga aatatgaatg ctaccaataa agccattata aagctagcat caaacaatca
     2641 gggaggtgtt ccactaatac tagatgagtt aggagcagca actcaaaata actttagctc
     2701 gttgatctat caattagctt caggagaaga aaggctaaga ttagattcta attgtcagct
     2761 tatgccacaa gagacttttt ctgttttgac ccttacctct tctgaagagc gtttggaaaa
     2821 ttatttcaat gagaaattaa ctggtttgaa agttcgatat atcgaatttt ctgatatcaa
     2881 gtttacaaag gatgcgaatc acgcagagaa agttaaaagt ctttcttcta atacttttgg
     2941 ttggggtata aagttatttc ttttgaaact ctttgaacaa gggattacaa aaattgaagc
     3001 aacttttgtc gaagctcaaa aaaaactatg ggctgtttta cctgaacatt ctttgaagca
     3061 acgcattggt aataatctag ctttgattta tagtggagcc ctcctaattc aagatatact
     3121 gggctatcct ttacgattag agcatataca gacggtgctt attaattcgt acgaacagtt
     3181 aattgtagat actagtttag gaagtgagca aacggtattt gaccgagtga aaacattgtt
     3241 ggtgatgaat agccataaat ttttaacaaa cagtgacaac aaacatacgc ccagtacgat
     3301 ttggggaaaa gtatcaattc aaaaaaatgg ggatattaag gtaaatattt ttcctatccc
     3361 gtttgaggac ttgttaaggc gagaatttca agtaaaggat ctaacctatc tttttaggca
     3421 attgcttgat tcgggtaata tgtgtgcaga aaaagggaga agaacgaaac gtattcacat
     3481 gaatcgtcaa gttcttccaa catacgagat tctattacct tcttcactca aatcgtattt
     3541 tcgattgtaa tttagaataa accaaagtat gaaagtgagg caccatgtta tggaaatatt
     3601 tcttattgat ttagcaaaac aactgattac ttatctgtat gatagcgagc aaacccaaac
     3661 ggaagtaaat gaagcttgat ctatgcttca tttacttcat tcaatcaaac atgaaagaaa
     3721 ggaaaattct aatgactaaa aataaaatga cactaaaagc agaggttctt ctgtatattc
     3781 aagaacattt ttcaaatcaa gcctttttta caaaaccaat ttacttggat ttcgaaataa
     3841 gaggggtatc tgctggatct atcgggggta ctcttcaagc gttgaagaac gaaggatatt
     3901 tagaaaatca ctttgtgcaa cgttcgttca atagaagagt cgtgaaaaaa tggtatttag
     3961 ttcattcata gagaggggaa tcaagcatgc gtgtggcttg ttatgatcga acgtctgttt
     4021 atgaagaagc gaaaattagt aatgacgctc aatatgatgc agtgaaagag tatgttttag
     4081 agaaatatga acaaattccc caacgatttt cagacaaagg agaatcaggg gaaaaagcgg
     4141 agcgtccagc ttttcgacag cttagagctt gggtttgtgc gtcagaagaa gagggggtga
     4201 tctatgtttg gcgttatgat cgattggcac gtcaagccaa aatagcctta gaatttattg
     4261 agttgtgcca acaacatcag gttcaagtgt attcgatttc tgaacctctt cctgcttctt
     4321 tctcacacgc gtcaacgaat aaactatttg tgcaattatt gttcactttt gcagaacaac
     4381 aacgaaacgt cattattgag aatgttcgtg ccggactttc ctataaaaag caacaacggc
     4441 aatatttgtc ttctgcggta ccatatgggt atcgtttagt gaatgggaaa atccaacaag
     4501 aagtacacga agcaaggatt gttcgctata tttttcaact ttatttgacc aaaaaatatg
     4561 gctacaaaaa gctttgtcaa cgattgacgc aacaaaagct tttcttccgc gaacgtcctt
     4621 ttcagcccta tcatatttat agtatcctaa aaaatccatt gtactatggc gaaatcaaag
     4681 gaggatcttt ggggaagtat ctaggaacct ttgaaccgat tctctcgaaa gcaacctttt
     4741 tacaagccca agagattcgc caatcaaggt gtacagctaa aaaagatacg tatctgtact
     4801 tattacgtca aaaaattcgg tgtcccttct gtggtcgtca tctgagttcg aagtatcaat
     4861 ggaatacaaa gaagacgaag acgcttcatt attatcattg taccgaccga agctgccaag
     4921 gaatttttct tcgggcagaa agggttgagt cagaagtatt ggaaaagata caggcttttt
     4981 ttcaatacga aggcgtctat caagccttga ttgcccaatt aacgcaacaa ttagccgaaa
     5041 agcgaaaaga tgaaaaacgc aaagtaatcc aacaaaagaa acaagaagca caatggatta
     5101 aggcatttga agagggcaat tgttcactgg aagaactcaa gcaacaactg caagctcttc
     5161 aaaagccgat gtctacttct tcgtttcttt cactccaaca gtgtgaagca aaagtgaaag
     5221 aatggcttca actaaaagac ctctctagtc gacaattatt atgtgactat gtttgtcgga
     5281 ttgaggtaac aaaggataaa acagtttgtg ggatttactt ccgacaactg acacaaaata
     5341 ttttagaaag gaacgaatga tctatggaaa tgatacaaac caaacgagtc gcattgtata
     5401 ttcgcgtgag tacacaagaa caagctttag aaggacatag tattcaagca caagaacaaa
     5461 atggacgaca gtttgccgaa cggatgggct atgaagtggt agaagtttat gccgatgaag
     5521 gaatcagtgg gaaatccaca aaaaatcggt tcgcctatca acgaatgatg aacgatgctc
     5581 ggaaccatcg ttttgatcta ctagtgattt ggaaattgac ccgtctcgga cgcaatatgt
     5641 tagatgtctt acaaacagtc gaggaattat tgcgatatga cgtaggatta cattcgatct
     5701 cggaacaatt tgacgtgacc acttcgacag gtaaattgat gttacaactc cttgggagtt
     5761 tcggtgaatt tgaacgcaac caaattgcgg agaatgttca aatgtcaatg caaagcttag
     5821 tgaaagataa aaagcgttat gcaggaggcc gacgtttagg ctatgtcagt ggtattgaca
     5881 cgaatggtca aaaacaattg gtcattgaac cagaagaggc gaaaattgtt cgtctgatct
     5941 atgcaaaata tttaggcgga gcagggtatc gagcgattgc caattttttg aatcgtcaag
     6001 gatatcaaac agtcaaaaag aatgcttttt ctaccacggc agtcaaagat atcttacgca
     6061 ataaaattta tgcagggtat ttagaatatg cgcgctacat caattgggaa accaaacgac
     6121 ggaaggggaa gaatccgaga ccgattttag tgaaaggaac ccatgaaccg attatttcgg
     6181 aagaagatta tcaaaaagta caagaacgcc ttgctttaga aggaaaacaa cccaaatgga
     6241 accatacagg tgaaaatgta ttgacaggct tgttacgttg tccagaatgt ggcgcaccga
     6301 tggcagcgag taatgtcaca aataccctaa aggatggcac aaagaaacgg attcgatatt
     6361 attcttgttc agtctttcgg aataaaggag caagtgtgtg tcatgccaat tcgattcgtg
     6421 ccgaacaagc ggaaacattt gtcgccgacc gattaaagga gatcgttcag ctaccagaag
     6481 ttcttccccg attggtggca gcgttaaatg aagaaatagt gcgacaaagc caaccattgg
     6541 aacaagaact cgtggtgtta ctggaacgaa aggaagaatt gaagactaaa attgaaaaat
     6601 gggaagctgc tttggaagat agtcctgaat tgtttccgat gttaaaagac cgcttagacg
     6661 aattgactga aaaacgacga cagttgcaca ttcgagaaaa tgaaatctta gggatctttc
     6721 aacaaggaga gccgattcaa gtcaaagatg tccaacggat attgactaat ttggatcgtt
     6781 ttcttgccca gagtgagaag aagcaaatca aagccttgta tcgtaccttt atagagaaaa
     6841 taacctttga tcctaaccat aaggagatgt tggaaattac gatgaaattc acgcatgccg
     6901 tggtacaaca attgaatgag caatatcaaa tagccgtatc caaaacaaag gatacggcta
     6961 tttttgtgtt aaaaacaccc tttacgttaa cgatctagtc taggttatat caaacaacgt
     7021 tagaggggcg ctaacgcttc tctagcgatt tgaaatcgtt agagaagtag ttagggaggt
     7081 ccttatcata aatagggata tttattaatg aatatttgaa atataattgt atataagaat
     7141 taaaaagagt ggggatagat atgaaaaaga tgggtaagtg tattttatgt gaaaaataca
     7201 ctgaacttac taaagaacat gtaccaccta aaaaatcagg gaatactgga ggtaaaaaga
     7261 ccagaactgg taatttagat gatttcctta agagcgattt tacaaaaggt gattttccga
     7321 aggggattaa aaggaaacct caaggaaatg tttattata
//
LOCUS       DARBFO010000113         6924 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000113 DARBFO010000000
VERSION     DARBFO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.116"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            94..1248
                     /locus_tag="SCK51_002588"
     CDS             94..1248
                     /locus_tag="SCK51_002588"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEG1253157.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="SCK51_002589"
     CDS             complement(1270..2022)
                     /locus_tag="SCK51_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1253158.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="SCK51_002590"
     CDS             complement(2386..5052)
                     /locus_tag="SCK51_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1253159.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="SCK51_002591"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="SCK51_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEG1253160.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2044 a         1422 c         1252 g         2206 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacca
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagcgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaa
//
LOCUS       DARBFO010000114         6643 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000114 DARBFO010000000
VERSION     DARBFO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.117"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..859)
                     /locus_tag="SCK51_002592"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="SCK51_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="SCK51_002593"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="SCK51_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEG1253161.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="SCK51_002594"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="SCK51_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="SCK51_002595"
     CDS             complement(2143..2373)
                     /locus_tag="SCK51_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HEG1253162.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="SCK51_002596"
     CDS             complement(2603..3421)
                     /locus_tag="SCK51_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1253163.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="SCK51_002597"
     CDS             3582..4271
                     /locus_tag="SCK51_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEG1253164.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="SCK51_002598"
     CDS             4285..5787
                     /locus_tag="SCK51_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1253165.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="SCK51_002599"
     CDS             5800..6276
                     /locus_tag="SCK51_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1253166.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="SCK51_002600"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="SCK51_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DARBFO010000115         6570 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000115 DARBFO010000000
VERSION     DARBFO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.118"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            127..579
                     /locus_tag="SCK51_002601"
     CDS             127..579
                     /locus_tag="SCK51_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253167.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="SCK51_002602"
     CDS             593..1282
                     /locus_tag="SCK51_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253168.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="SCK51_002603"
     CDS             1407..3758
                     /locus_tag="SCK51_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEG1253169.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="SCK51_002604"
     CDS             3838..4233
                     /locus_tag="SCK51_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253170.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="SCK51_002605"
     CDS             4558..5175
                     /locus_tag="SCK51_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1253171.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="SCK51_002606"
     CDS             5216..5515
                     /locus_tag="SCK51_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEG1253172.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="SCK51_002607"
     CDS             5549..5716
                     /locus_tag="SCK51_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1253173.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="SCK51_002608"
     CDS             complement(5761..6366)
                     /locus_tag="SCK51_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1253174.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2110 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt
//
LOCUS       DARBFO010000116         6567 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000116 DARBFO010000000
VERSION     DARBFO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.119"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(7..1164)
                     /locus_tag="SCK51_002609"
     CDS             complement(7..1164)
                     /locus_tag="SCK51_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1253175.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1167..1523)
                     /locus_tag="SCK51_002610"
     CDS             complement(1167..1523)
                     /locus_tag="SCK51_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253176.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="SCK51_002611"
     CDS             complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="SCK51_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HEG1253177.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2991..3830)
                     /locus_tag="SCK51_002612"
     CDS             complement(2991..3830)
                     /locus_tag="SCK51_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG1253178.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            4015..4902
                     /locus_tag="SCK51_002613"
     CDS             4015..4902
                     /locus_tag="SCK51_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEG1253179.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(5486..6181)
                     /locus_tag="SCK51_002614"
     CDS             complement(5486..6181)
                     /locus_tag="SCK51_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEG1253180.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(6165..6563)
                     /locus_tag="SCK51_002615"
     CDS             complement(6165..6563)
                     /locus_tag="SCK51_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEG1253181.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         2065 a         1301 c          966 g         2235 t
ORIGIN      
        1 cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat
       61 ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta
      121 aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac
      181 aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa
      241 tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat
      301 gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa
      361 ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa
      421 aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc
      481 tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat
      541 cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt
      601 tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc
      661 tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag
      721 gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg
      781 ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat
      841 ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag
      901 taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt
      961 ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa
     1021 tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg
     1081 attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct
     1141 taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat
     1201 tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt
     1261 atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt
     1321 tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga
     1381 atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca
     1441 tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg
     1501 atatcaataa attcttcaaa caaagtacat actccttttt atctattatt tagacttatt
     1561 ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag agatactacc
     1621 atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt
     1681 tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca
     1741 ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt
     1801 agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct
     1861 cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta
     1921 ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca
     1981 attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt
     2041 ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct
     2101 gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta
     2161 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt gctactttca
     2221 ctactcgtac tctccgtact gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc
     2281 gtactctctg tactgctttc accgctcgtg ttttctgtac tgctttcact acttgtgcta
     2341 tccgtactgc ttggcggtcc aacaactgtc caactgcttg gtttcaaatt ctccgtgatc
     2401 gtcatctgtc catcataaaa aacaatcaaa tcaactttat ctttactctt gatctcatca
     2461 atcgatagtt ctgcagaaat cattattgta tactcaatac cagcggtcca ttgtacgtct
     2521 tttaaataaa cttccccaat aactttttct gaacttccat taccatccca accatatgtt
     2581 agaacagcat gtacatcact agcattcacg cttttgtttg gtttgactgg aatagtaatg
     2641 acttgttgtg tcatgtcata aacaatccga tcagttttca cagtaaatga aagattctca
     2701 tttgcccgtt catttgcaga agtactgatt ccaatcattg ctgcaattat actcagccca
     2761 agaataacaa ataatacggt cgctgttttc ttcatttcta tcactctcca tataattacc
     2821 ttctatttga taaaggaatt atataaaatg aaaatgatga gaacacgaca gtcagttgtg
     2881 aaaatctaac aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa
     2941 tgacagactt aaaaaagcaa gaccgatatc agccttgctt tcttcatctt ttattgataa
     3001 acagcagtgt atttcttcat ccgctttgtt ttatatgcat agagtaacaa accaacaatc
     3061 aagactaaca agaataatgt agttgatata gatgcttcag ccccaaagtt tcccccagta
     3121 attattgtat tttgcgattg gatcagagtg gagaaaaaac cttgttcttg cgttccgctg
     3181 actggtatac ccaaaatagc tccttgtgtc caattccaag taatatgagc accaatagcc
     3241 gtaaataaac ttcctgtact gtagataagt gcgctgaaaa agcaacctgc taaaaagata
     3301 ttgaaaactg ccagaaatgt catgttagga ttcaatccgt gcaaaagagc aaataatgca
     3361 gcagatccaa tcattgctat taacggacca aatctaagca tctgatgctg aaaaaagcca
     3421 cggaataaaa gctcttctga tgcccccact gcaataaata aaataaaact cgttatcagc
     3481 ggcacaatgt ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata taatggacta
     3541 gtaataagca gaaacactcc aattccaaca acgagacctc ccgctaaata cccccagtct
     3601 tttcctcgaa cagaaacacc ataatcttct attctttttc cttcgacctt actagcaaga
     3661 aataaagcta gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc tccaatccat
     3721 tcactatttc caataaacat atccgcaagt gcattattga caagaattcc taaaacaaaa
     3781 ccaactaaga ctattaaaac agatccaatc aaccgaataa tcctgatcat ctttcatccc
     3841 tcctttatat taagtttaac aacccttttc tttcttgtct cgtgaaaact atgtaaatgt
     3901 tctccatcat agagagaata gatttttacg aactaagaat atctacagca accttacgaa
     3961 aatgaaagac tgtctcccta attcttctgt tagactgttt ttaataagga aaggatggga
     4021 aattttttga aaaaacaact actaattatg tgcctttcag catgtgcaat tttactattc
     4081 tcaggttgta gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac acttacagtt
     4141 caggattttt atgataaccc aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg
     4201 attgtcttca aagcatttga tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca
     4261 aaagaatata atgaacaaat caaaaaactc ggtccaaact acaaggaaca attaaaagca
     4321 gttggccaaa cagaagagac atataagaaa ctctttaaac agatgctggc tttccaatac
     4381 ggtttgaaag ccaatgtaaa attaacagat aaagatctag acactgcttg gaaagaattc
     4441 tatccagaag ttagtacaca gatcattcta ttttcgacta aagaagaagc agataaagca
     4501 aaaaaagaag caaatgaagg ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat
     4561 aaaacattaa aggaaactga tggaaaaatg aattttgatt caaccaatcc agagattcct
     4621 actgaagtaa aaaaagcagc atttaaacta aaaaacgggg aagtttctga tatcattcct
     4681 gtaacagatc caacgactta tcagcaaagc tattatctag taaaaatggt aaaaaaacag
     4741 gataaaggtt ctaataaaga caaatacaaa tctgagctag aaaaaattgc taccgaagca
     4801 aaaactcttg atacggaatt catggataaa acaatccgta aagtgatgaa aaaagacaat
     4861 gtaactatta aagatcctta tgtaaaaaat atattcaaat aaacaaattt gacccaattg
     4921 gggatgtggc aaaatttgag aagtgatttt tggaaattcc ttcttatttt tgaggcaaac
     4981 cacttttgcc acaacttcat cggggccttt ttttatttgt accaaaacaa atcactggat
     5041 ttgttacaat catgaggata taatttaaaa aagtttttta tttctatcac atgaatcaat
     5101 gcgccattaa gtagattctt tcatgcttcc ctattttttc acttgccgta tcgcttcaaa
     5161 aacaactatc ataaactcaa actcgactga aaagttattt taaaaaagac taaaaatatc
     5221 ccttctggac ttgttcctct tattctgtta tactagagaa acacgaaaga tatttttatg
     5281 cgacactttt catttgtttt tatgaaattt attcaaaact ggcgtaactt cagagttaca
     5341 aggatgtatt agaggttagg gaatacatcg tttaaatgag aaataagtaa tagtcaccca
     5401 aactttaaat taatgaatat tgaatcgtca gatagcctcc tcgagttttc gagaaggcta
     5461 tcgtttattt tatggagaaa ttgattcaga agtaatcagc gacctttttt agttcaccaa
     5521 tcgtttctgc ttggaaagtt tgcacccaat cttcttggaa gcccctcact gctttatcaa
     5581 tcgagggcat ctctaatact tgatctagta gctctttacc taacgttatc gcatgagctc
     5641 cagatcgaat ggctgtagtg acttgttcac aatttttgaa actagctgct aatatttttg
     5701 ttggtctttg cgtacgttcg atctcagtcc gcaacgaatc aatcactttc atagcatcaa
     5761 tgccttgatt ttgcatcctg ttataataag gtgcaatata atctgctcca ttattaatag
     5821 ctaaataccc ttgtaattct gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt
     5881 tcaacttttt gatcgcttga aggcctgctt gattcaccgg caccttgata aacgtatctt
     5941 ttcccagttc ttctatgata actcgagcat cctctatcat ttcttcttcc gttcttccta
     6001 ttgtctgaat atggatagga atctgtccga ttattttttt gatttctttc atatgcgaga
     6061 agaagtttga cgctccttct tttttcagaa ttgtaggatt tgttgttacg cctgttaaat
     6121 tcaaaaactg ttgacaagaa taaatacttt ttgtatttgc tgtatcaagc atgatttcca
     6181 tatagctttc ccccattctg atcctcttga tcgattttaa tgattgtccc ctttcttaaa
     6241 tgcggcattt ccttatcttc tacatacatt gttcctggaa gttccggctc atgagatcca
     6301 tcaaatttta tcgtgatatg tcctaattca ttcaaattag tttggacaac atctcctact
     6361 gctgtgatta catacatttg tttatcaaaa cagagcttca tatcacaagt tatttctttt
     6421 cttacctgat tcaattcaat cttataacaa taatctgtta atgtttccgg tgcttcatct
     6481 ccaaataaaa tcattatttt ttctttcctg aatacttctg cttctatacc aacatctgtt
     6541 acaaccgttt caaagatcgt catctct
//
LOCUS       DARBFO010000117         6519 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000117 DARBFO010000000
VERSION     DARBFO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.120"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(109..324)
                     /locus_tag="SCK51_002616"
     CDS             complement(109..324)
                     /locus_tag="SCK51_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEG1253182.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(343..579)
                     /locus_tag="SCK51_002617"
     CDS             complement(343..579)
                     /locus_tag="SCK51_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEG1253183.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            837..2990
                     /locus_tag="SCK51_002618"
     CDS             837..2990
                     /locus_tag="SCK51_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEG1253184.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2959..4374
                     /locus_tag="SCK51_002619"
     CDS             2959..4374
                     /locus_tag="SCK51_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEG1253185.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            4407..5441
                     /gene="sppA"
                     /locus_tag="SCK51_002620"
     CDS             4407..5441
                     /gene="sppA"
                     /locus_tag="SCK51_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEG1253186.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            5443..5964
                     /locus_tag="SCK51_002621"
     CDS             5443..5964
                     /locus_tag="SCK51_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG1253187.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            5961..6347
                     /locus_tag="SCK51_002622"
     CDS             5961..6347
                     /locus_tag="SCK51_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEG1253188.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         2292 a          960 c         1191 g         2076 t
ORIGIN      
        1 agctttagta aaaagttctg tccattccaa acttttgaaa agataaagaa catacctatg
       61 ttcgtaatcg acacaaacca atcttgaggt ttgatatttt ttaattgttt acgcaccggt
      121 ccacatgctg aatccagcac ctaataaagc tccacctaca ccacaagcag ctattgcata
      181 aggtcctccg gctgaacaaa gttttactcc tgctgttgcc ccagaaaaag tagtgcctaa
      241 aactcccacg gcactcccac cttgaatatt tttcaactct tctgaagata atataaatgt
      301 tgtttttgtg tttttaatat gcatactact tattctcctt ttttattttt ttcgagcatc
      361 ccagccagct gcagcacctc cagcgacagc tccatataac cctccaacaa caccgcaagc
      421 tggtccgcaa attgctgttc ctaaacctgc accacctact gcccctgtgg taagtccacc
      481 tacaacttgc cccaattttc cccaagcatt attgcctcca ccaacagttt gtgcatttcc
      541 acaatactta gttcctttac attttgcatg tttaaacacg cttctttaaa taatattttt
      601 tacggtgcta ctgtaacata cgtatttgat taacagtaaa aatcttcctg aaacttatca
      661 agtttattct tgaatagtaa aatttccaaa ataaatttga ttcaggaatg aaaaagttag
      721 tgttcaagaa ctgttttcaa agtttttgtg gaattatgag acaatatttt cactgtatta
      781 aagctatatt gagaattttt ctataaaaat tttttagaag gagttgtgtt agataagtga
      841 taaaatcatt tccttttgtg cagcaacaag atgaaaaaga ttgtggtgtt gcttgccttt
      901 cgatgatttt aaaatatttt aaaactgata tcccgattca taaattacgg gacctatctg
      961 ggactgattt agagggtact tcagcatttg gattgaaaaa gacatttgaa aaattgtctt
     1021 ttgattgtat ggctattcaa gcagatagtg agatctggaa agaaagagat cttatcttac
     1081 cattaattgc acatatattg gttgataaca gctatatgca ctacgttgta atttataaaa
     1141 cgaagggaaa cgtactatgg attgcagatc ccgctaatgg aaaaaataga aaaacgattg
     1201 aagcgtttca aaaagaatgg acaggtattc ttttattacc tatgcctaga gaaacttata
     1261 ttcctattaa agaaaaagta gctggattaa gttctttttt gcctataatt tggaaacaaa
     1321 aaggattagt attccacatc gttttagcat cgattttcat tacgtttttt ggcataggaa
     1381 gctcatatta ttttcaaggt attcttgatt ttttcatccc taatcaagca cgttcaactt
     1441 taaatataat ttccattggc ttgatcgctg tctatttatt tcgggtattg tttgaatata
     1501 gtcgtagtta tcttcttatt attttaggac agcgaatgag tatggcaatt atgcttcagt
     1561 atttcaaaca tgtgttaact ttaccaatga atttttttgc tacgagaaaa tcaggggaaa
     1621 ttatttctcg ttttcttgat gccagtaaga ttattgatgc tttagcaagt gctacgttat
     1681 caatattttt agatattggt atggtgttgc ttgttggggt tacgttagct attcagaata
     1741 gtacgttgtt tttaatcact ctatgctctt tgccttttta tgtagttgca atattcgctt
     1801 tcgtgaaaag ctatgaaaaa tcgaacaggg aagaaatggc agccggtgca gctgtaaatt
     1861 ctagtatcat agaaagtctt aaaggaatag aaacaattaa agcatatagc ggtgaagaga
     1921 aagtatataa ccgtatagac catgaatttg ttaaattaat gaaaaaatca tttcgtacgg
     1981 ttacgcttga taatattcaa caaggaatta aacatttgat tcaattaatt agcagtgcat
     2041 taattttatg gctaggttct tattttgtta tggataatag aattagtcta ggtcaactta
     2101 tcacatacaa tgctttacta gtatttttga cagatccttt acaaaacatt attaatttac
     2161 aagtgaaaat gcaaacggct caagttgcaa ataatcgttt aaatgaaatt ttcgcgattg
     2221 attcagaaaa atcgaaccag tcttttgaat atatgctttc tgaacaatta tttcaacaag
     2281 atatcactgt aaaaaacgtt tcattctcgt atagcatgaa ggccccaacg ttgaacgaaa
     2341 tttcatgtac aattccttcg cacagtaaaa cggctatagt tggagttagt ggttctggaa
     2401 aatcaactct tgcaaagtta tttgttcgtt tttatgaagc atcagaagga tcgatccatt
     2461 atggaaacat taatcactta gacattcctt ttcagatatt acgcgatcga gtgacatatt
     2521 tgcctcaaga gtcatttttc tttagtggaa caatcattga gaatcttata ttcggtttag
     2581 atagcccacc aagtttcgaa caaataatag aagtttgtga gatagtccaa ctaaatgaat
     2641 ttattaatca acagccatta cgttacgaga cattgttgga agaaggtgga aataatcttt
     2701 caggagggca acgacaacga ttagccttag cacgggcttt attaaaaaat ccggacatat
     2761 taattttaga tgaagcaact agtggattag ataccttgtt agaacatgca attatgaatt
     2821 atctcctcaa tttaaaagaa aaaacgatta tttttattgc gcatcattta ccaatagcaa
     2881 aagcatgtga tcaaatttta gttctccatg aagggcattt ggtagaaaaa ggaacgcacg
     2941 atgaacttcg ttttgccgat ggtatctatc aaaaattatg ggaaatctag gagagggaaa
     3001 caatttatgc agaataataa ttggttcgaa cattctagta tctatactaa acaacatcat
     3061 agcttctacc gttgggtgtt ataccctgta attctttttt tatgtattat tggattgttt
     3121 ttaacctttg ctaaaaaaga ggttgttatt cggacaacgg ctcaattgaa ccccgaaaaa
     3181 atagagaagt tacaagtgcc attagaagct aaaataatgg aaaacttcct aaaagaaaat
     3241 caatttgttc ataaggacga tgtgttggtt agattggatt gttctttaat agagaatgaa
     3301 aaggctcaga tagaacaaga aaaccaacgt attactcaac aaataaagat ggctcagcta
     3361 tttattgaaa gtataagtaa aggaaaaaat ttgttttcaa cggatgacag ttttggctac
     3421 agtaatcaat taaagagcat gttgtcagaa aaagaatcac tccgctacgc tttgaagcaa
     3481 agtgaattaa atgatcaaaa gcaattagaa gtatacgaaa agacaaaaag acaactagaa
     3541 aaacaaattg agagttcaga tagtaaatta caagaatggc aacaagtaca ggtagcttgg
     3601 agtaataatc aatcattaaa agatttttca aaagaaatga tggcaaacta tgagaattgg
     3661 caagaacaac taaataatgt ttctgatgat caaaaaaatc aagtgaaact gacaatttca
     3721 gcaagcataa atgaacaaat tgagcaacta aaaaaagaag tagaacagta tcagtcagaa
     3781 aaagctaaat tagttaaacc aactacttct gagaatgaca gaattagtca aacggaaaaa
     3841 ggaaagcaag agctagaaca agcaataaca actgtaaaac aaacagttac agagctaaat
     3901 gaaaaacaag agaagaacca gtcagttatc aaaagtttgg acgatcaact ttcaaaagga
     3961 atactaaaag ctcctgtatc tggaacggtt catttaaatg aagaaacaaa gggacaagca
     4021 gaactagcca aaggaacagt gcttgcagaa atttatccga tccacgaaaa atcaaaaatg
     4081 aaatttacag cacttttacc tgcgaatgaa agtacctata ttaaagaagg gatgaaggtt
     4141 cattttaaat tggatcaaaa aggtggggct ccgattacga tcgacggaac tttagatgaa
     4201 atttcagaaa attctactgc cactgagaaa ggtgtttttt atgcagtgaa aggaacgtta
     4261 aaaacaactc aatcgtttcc gtttcgttat ggattaactg gagagttgtc tttaattgta
     4321 ggagaaaaaa catattggca acaaattgtc gatgtgtttc taaataaaaa ataattattt
     4381 ttactaagaa tataggagga gtttatatga ataaaagaag atggattgct gtgggagtag
     4441 cgtgcggatt attgttacta tccattatcg tcgcgctaat tccaggaaaa gataaagagg
     4501 aagcatcaaa tacaacccta acaggaataa ataagatttt ttacggatct aacgaaatca
     4561 cagaagaaac gttagaagaa ggcgcatcaa ataaaaagat tgtcaaacta tccgtcaatg
     4621 gagttattgc agatacaggt gaaagcaatc tttttagtag ggaacaatat aaccatcaga
     4681 attttttaac acagctaaag aaaattcaag aagacaaagc ggttaaggga gtgttattag
     4741 aagtaaattc acctggggga ggaatatatg aaagtgctga gattgctaaa gaaatggcaa
     4801 aaattaaaaa gctagatatt cctatctata ctgcatttaa aaatacagcg gcaagtggag
     4861 ggtactacat ttctgctggt tcagataaaa tatttgctac ggaagaaaca acaactggat
     4921 caattggtgt cattatttct ggtttaaatt attctggctt attagaaaaa cttggcgtaa
     4981 ctgatgcgac gtataaaagt ggtgcattaa aagatatgat gtcacctcaa cacaaacctt
     5041 cagaggaaga gaataaagtt atacaggaat ttgtgatgag tgcttatgat cgttttgtca
     5101 atgttgtagc aaaaggaaga aatatggata ctaatgcagt gaaagaactt gcggatggaa
     5161 gaatttatga tggtaaccaa gcagtagaaa atgggttggt ggatcaaatt ggttactctg
     5221 aagatgcatt agattcccta aaaaaagaga agaaattaac tgatgcgacg gttatagaat
     5281 ataaaaatga cacaactggc tttgcttctt cttggttagg taataagatt gctgaatggc
     5341 aagggctaaa ggcaactaca tcagatcgtg taatttcagt ttttgaaaaa cttggatttt
     5401 ctgatactcc aaaaccaatg tattactatg gagggctata aaatgaataa gaagagtggc
     5461 attgaaactt tgcctaatta tgtatttgcg ggattttggt tacgattttt tgcttttctt
     5521 attgatttaa tttgtattag catcatcacc catgctacta taggtctctt ttacaaaatc
     5581 atgggactgg aatctgacaa atccattatt actttttatg gtatagcgtc attagctatt
     5641 tatttaacct attttgtact tttgactaag ctgaattcag gacaaacaat tgggaaaatg
     5701 atttttggga taagagtagt gagctttgag gaaaaagagc taagttggca aactatcttt
     5761 attcgtgagt gtatatgtag gtttatatta aaaaattttg tatttcaaat tgggtatttg
     5821 gtggctgctt tcacgcccaa gaaacaacat attggggatt atttttctaa cacgtcagtg
     5881 gttgttattc gttcattaga agctattaac tatggtcaaa aaatttctaa ttctaaaaca
     5941 cttaatctgg agagaattaa atgaataaga gaaaaacaga gtttattttg attttaatta
     6001 gtggaataac agggatttta tgtagtcttt tagcattgat gggtggtatg tttctatctt
     6061 ctattgattc aactacttta aatactacag aactaaccag taaggatata gatatgttag
     6121 tcaatctaag cgggaatgtt gttttatttg gaggaattac cctagttttt tcgataatga
     6181 ttttcataat aggtttttta gtaagaaaaa atcaaaaaac agtggtctat ggtattagtt
     6241 tactcatttt atctattatt ccattttttc ttttaacatt attgtgggta attcctggag
     6301 ttttaggtat tattgcggga ttgatgcttc taataagaaa atcttaatta ggcaatgtgt
     6361 agacaacgtg aaacgattaa tcaattaaaa gctaaatacg ttttgagaaa tgataacttg
     6421 tagtcgatgg aagacatgta gataaaaaaa ctacgtgtct tttatttatt ctaatttttt
     6481 cgaaaaaaag acactctctg attgttgtat taatggaaa
//
LOCUS       DARBFO010000118         6496 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000118 DARBFO010000000
VERSION     DARBFO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.121"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            149..1060
                     /locus_tag="SCK51_002623"
     CDS             149..1060
                     /locus_tag="SCK51_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HEG1253189.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1547..1813
                     /locus_tag="SCK51_002624"
     CDS             1547..1813
                     /locus_tag="SCK51_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253190.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            1885..2226
                     /locus_tag="SCK51_002625"
     CDS             1885..2226
                     /locus_tag="SCK51_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253191.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2281..2700
                     /gene="ssb"
                     /locus_tag="SCK51_002626"
     CDS             2281..2700
                     /gene="ssb"
                     /locus_tag="SCK51_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG1253192.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            2777..3073
                     /locus_tag="SCK51_002627"
     CDS             2777..3073
                     /locus_tag="SCK51_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253193.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3088..3519
                     /locus_tag="SCK51_002628"
     CDS             3088..3519
                     /locus_tag="SCK51_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEG1253194.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            3701..4006
                     /locus_tag="SCK51_002629"
     CDS             3701..4006
                     /locus_tag="SCK51_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253195.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
     gene            4410..4898
                     /locus_tag="SCK51_002630"
     CDS             4410..4898
                     /locus_tag="SCK51_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1253196.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            4885..6189
                     /locus_tag="SCK51_002631"
                     /pseudo
     CDS             4885..6189
                     /locus_tag="SCK51_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347576.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            6232..>6496
                     /locus_tag="SCK51_002632"
     CDS             6232..>6496
                     /locus_tag="SCK51_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1253197.1"
                     /translation="MTKLGITLDDNIYDEKILLSGGEKQKISLARALIKSASVIILDE
                     PFRNLDNETRDNLRDYILNTKKEVCYIIITHDHILDNFLDSNDE"
BASE COUNT         2457 a          850 c         1241 g         1948 t
ORIGIN      
        1 tggagtaaaa caagtgtaga ggaaacttga agacttgttc gaacacaaac ttacaagaga
       61 ggggagtaaa gcagttgcag aggaatcagg aagatttgtt cgaacagaaa cttgcaagaa
      121 agcattatta aattgaaggt gatgaaatat gaagtattta ttgcatggct attttattga
      181 ttattctact cgcaatagta gtactttagc gattctatgt ttgcttcatg aattgagagt
      241 ggttactaga aaacagctat acaaactatt gaattttgac agatcaatta gtaaacaagg
      301 agtaacgaat gctttagtga gattatatga aaaacagtta atcgatcgaa aaaaatatgg
      361 aacagaaatg tattattatt tgacgaaaga gggacatcaa agcataggtg ggtattatac
      421 gttgccgaaa gttcctgaat ataatttgaa tcaccattta caaattaaca attatttgat
      481 taaaggaatg gcattggcaa aagaccaccc acatttaaaa cggattattt cagaaagacg
      541 taccgtctat gaaacgaagg atttaaatcc taagacaaaa ggaagaaaat tttttgtgcc
      601 agattttatt tttcgttttc gagacaaaga aaatcatgag atcaattggt cctttgaaat
      661 tgagctaacg ttgaaaacac gtcgtcgcta ccgtgaagcg atttttccga aatatattcg
      721 tgagttgcga acacgtcctg aatcacattt aatttatgtg acacctagtg gcgcaattca
      781 agaagaactt catgcgttta agcgtatttt tttcaagcgc gaaactgaaa aagatgcgag
      841 tcaacaagaa ttgtttgagc ggttgcatat tttttcagct ttagagtttg aagagggatt
      901 aaaagcatta ttagagaatg atccagcagt caattggcgg acagtagaac gagaaaaaat
      961 ttatccgaat ggcgctgttc aaagtcgttt gaatttattt gaagaatcaa ctgaaaatga
     1021 ggaaaaacca aaagtgaagt ttacgcctga agtattgtaa aagagggtgt tcgcttcgct
     1081 ctttttttgt gccagtttgc tggcaccgtt cagacgcaag cgtctgaacc ggctcgtttt
     1141 taggaaggac taacttgttt gatttatccg ttgtcaaaga agtggacgtt cgagagtccc
     1201 taatcgtata gcagtcattc ggcgagtata acctaaaaca taacctgcca attgccaagg
     1261 gtaaaatgca cgctgcgcgc ccttggcaat cgtcagaaca tgttttcgag ccggttatgc
     1321 cgaacgaccg ctatacgaaa agggcctcta aacagaagtc cactttgatc cttgacaact
     1381 gaaacccaaa atcaaacaag caggccttcg tctaaaaacg agccgaatga agaccttggg
     1441 ggttgcaccc ccaaaccccc gtgcccccga cacactaccg aaaaggtggg ggtgggtggc
     1501 gaaaaaatca aataaaacat tctattcaag aaaggaagaa acaaccatga caactaaaaa
     1561 cgagcaactg aaagaacagg caagaaaaga gttgcaagaa agaggtttga ttattgaggg
     1621 gagctttgaa ggagattttg aaacctatat tggttgttat gcaagaccga taaacaaacc
     1681 tacagcctta gacccaatga acgaacaaga agtaaaagaa caagaaaaac atgcagtgaa
     1741 tggttttccg caagatttca ctgaatggta tgagtgggaa attaacaacg ggaagcttga
     1801 aaacttctta taaaagtcag atgacgtgca aagagaatga ttctctttgt acggtaaaaa
     1861 tataaagaaa gaaggaaaaa gaaaatggca aaaaacacac cttatcaagt aggagacatt
     1921 ttttatagta gttggggcta cgaacaaacg aacgtgactt tttggcaaat tgtgaagtta
     1981 acagaaaaaa cagcatggtt tcagcctatt caaaagaaaa cggtcgagca ttattcgtcc
     2041 atgagtggaa aagttgttcc aatgaaagac aagtttatag agtatccttt agcaaaaact
     2101 aaagacagta ttgtaaaaaa acgaattgat ccagaaacct atcctgatat gttatgtggt
     2161 tatcgtagtt gggacacgtt tcatagatat aaaaatcaag ctgtttatga aagtagttat
     2221 tattaagggg atagtttccc ctttcaaaaa taataaatag aagaatggag agaaaaaatc
     2281 atgatgaatt cagtgaattt agtaggacgg ttaacaaaag aagtggattt aaaatacaca
     2341 ccaaaaggca atgcgacagg cacatttatt ttagctgtta atcgaaatta tacgaatgca
     2401 aacggcgagc gtgaagcaga ttatatccgc tgtgtcattt ggcggaaagc tgcggaaact
     2461 ttagcaaaat atactggaaa aggaagtttg attggcatta atggacgtct tcaaacgagg
     2521 agctatcaga atcagcaagg acagaccatg tatgttacgg aagttctagt gacagatttc
     2581 tatttgttag agagtaaaga aatcaatgag caacggtcaa aatcaatggc gaataatcaa
     2641 aataattctt caattccagg aaatgagatt caaatttcag atagttattt gcccttttaa
     2701 gtgaaaaata attttgttct atacctgatt tcttcaggta tagaacaaaa aataaataga
     2761 gatgaggcga ttttttatga aaatcattga cagttacgat caacgaacca tgcaatattt
     2821 gggctcgttt gattatacag atgaaaatat tattaattat gttgcaagtt tacctcctta
     2881 tcaaagcgtt cgcttggtag attttgcaag tgatgaattg gtattaacaa cgatgggcaa
     2941 ctttttagat tatgtcggag acaaaaaatg gttggaaaaa attttgtcta aattgattgc
     3001 gaaacaaaca ggagaagaac ccataaatgc gattcaattg ttcaatcgat atgaacaaga
     3061 aaacaatgat taaagaggag agaaaaaatg aaaagattaa aacgaagtat ttttattgtg
     3121 ctagcattga ttgattttgt tgggacaggt tgtgaagcaa ttgacgttca aaaagctccc
     3181 ttatcggaaa taaaacaagc aataacaaaa caagatgaag aacaaccgct aaatacacaa
     3241 gtagaaatag tgaatcaaaa taaaccgagt ttttctgaag aagatttttc gttagaaaat
     3301 ggaaccaggc aatttttttc agatttggat cgtttaaatc gggtaggtgt agcaagtgca
     3361 cttttgcata aaagcaggat gccaacagaa gaacgagacc gattatacat taagccaact
     3421 ggctggaaac aaaagaaaat gaagaatggc gattatcttt ataatcgttg tcatttgatt
     3481 ggctatcaac gtgcgacaag tttccccata agtatgtaat acgaaacggg aggattaatc
     3541 ttaagattaa tcacaactaa cctatttgac agttggaatg aaagctatca tgtctgctga
     3601 atcaactggt ctgatactcc tacatgcaat aaagtctact aggaagttat tgtatgaagc
     3661 taggtaaagg cggcagaaca atttctaagt gtggaatcat atgaagttgg atatgccggg
     3721 acgctataaa ttactcaggg tgagactgtt tttaattaaa ctgacgaact tgcgaatgta
     3781 cgggtctata accgacttat attcgaaaga tataagattg cgaatgcaaa gaacattatc
     3841 gaaaagtaag aatgttggtc atgaaattcg agatggaaga ctagcggtgt atctgtcaag
     3901 agttggtaaa acatgtttga ttgattcaga tgagagtggg aatgcgacaa aacgttttac
     3961 agaagaaata gagcaactag cagaactgaa tatacgatat ttttaaatac tttagcggag
     4021 aataaaaaaa tcctctacag aaaactacgg agggcaacat gtgctattgt ttcaacaata
     4081 catttttaat ttttaccata acaaaaaagt acttgaaaaa aaacagaaat atgttaaatt
     4141 aaaaatgaaa aaatggaagc ggttacttat tcttttgaat ctaagataaa aattcaaaag
     4201 aataacataa cccaaatatg attatacttg agattctaaa tatttatcga aaaggaattt
     4261 gttttaagga aaaatgattc tatggacggt tattgattta tatttaccaa atggctaata
     4321 aaatagtatt tcttatattg tatagttttt gtttttatgt gaaaacatga gcaatagaat
     4381 tgtactagta ggataaggat ggatggaaaa tgaaaaaaac tatttttgtt ttagcgattt
     4441 ttatatgttt tgggctactt ttggtaggct acttgttgtt taattctaat gaaatgagaa
     4501 ctatttctac gaatgacttg ttaaaaaaaa gtgaattaaa ggataaaaaa aaagaatctc
     4561 ttgcctttat aatgtttact caagaagagt gtgagtattg tatagcaata aatccaatca
     4621 tagatagtgt aaataaggaa actttgaata agataaagaa atatgatata tctgtagatt
     4681 ttcaatctaa acaaaagtgt aaaattaagt ttgttccgac tattgttgca tatagtaaga
     4741 ataaagaagt ggggagaatt gacaatgaat tagataattt gtatcgaaaa aagcaagatg
     4801 gttcaattgt aatagacaag aaaaaaacaa agacattgct tatagattgg atgaaaaaaa
     4861 tggataaaca aggagaatat ttgtatggag aagattaaac agacatttcc gttaattaat
     4921 aaatattgga ataagaaaaa aatttttata ttttttatta cgtttctgct gtcatcttta
     4981 ttttctctaa ttttcccgta tctatttagt aatgttattg atttatattt agagaatgag
     5041 ttatcacaga atattgtgcg aatagtaacg attcttatat ttcagttatt tttagtctta
     5101 attaaagaag tgtatggata tataaaaact atttatgcat ttgaagttga tttatcaatg
     5161 aaaattgatt ttcttaaggt tttacaaaaa aaaaatcttt ttttttcgca aacgaagcat
     5221 tcaggagaaa ttcaatttag gatgttttcg gatatttcta ctatcgtaaa tactatcttt
     5281 attttttctg ttgaattacc tgtgataggc gttttagttc ttgtagcggt atttattata
     5341 agtaaagtga atcttatttt tctgattttt tcactaataa tgatgggagc tatggtgtta
     5401 cagtataatt tatttcaatt accgcttaag aaaacaatcg agaatacaaa aagtgttaat
     5461 caaagtatta caggagaatt gaatgcacat tttgatcaat tagaaattat aagcgcatat
     5521 aattttgaga atatctctat tttaaaattc gattctagat ttaaagaatt atttaatgtt
     5581 ttcaaaaaac aaaataaatt actaacactg ggaagctgtt tggcacaggt tattttccaa
     5641 ttatggattg gaggaatatt tcttattgat gttattttat tagctaaagg aacaataagt
     5701 attactaatt ttgtaatagt atttatgtac gctaacatgg tccctacttt atggactact
     5761 ttaattagta ttatttggaa atggcaagac tattcagtgt cattaaatag atattacgaa
     5821 tattattcaa ataacaacaa agtggctggc acaaaaaaaa taaataaaat aaattctatt
     5881 actggtcaat cattaaattt ttcatataac aaaagaaagg tattaagcaa tataagtttt
     5941 tcaactgaaa tgaataaact attcatgata tcaggagaaa atggaacagg gaaaacaaca
     6001 ctgatgaaat tatttactaa acagttgccg gttagtaata atcaattatt cgtcaacggg
     6061 atagatatta atatgattga taacgaatca ttaagaaggt taatttcaat cgcaacacaa
     6121 ataccatata taattaatag ctccgtgaag gagaatattt taatggggac taaaaactat
     6181 gataaaaaaa aattcagtaa attaatagaa ctattgggaa taagtaaatt tatgacgaaa
     6241 ttaggtatta ctttggacga caatatttat gatgaaaaaa tattattatc tggaggagaa
     6301 aaacaaaaaa taagtttagc tagggcgctt attaaatcag cttcagttat tatattagat
     6361 gaaccattta gaaatttaga taatgaaact cgcgataatt tgagggatta tatactaaat
     6421 acaaaaaaag aagtttgtta tataattata actcatgatc acattctaga taattttttg
     6481 gattcaaatg atgaaa
//
LOCUS       DARBFO010000119         6376 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000119 DARBFO010000000
VERSION     DARBFO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.122"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(182..1144)
                     /locus_tag="SCK51_002633"
     CDS             complement(182..1144)
                     /locus_tag="SCK51_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1253198.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1146..2585)
                     /locus_tag="SCK51_002634"
     CDS             complement(1146..2585)
                     /locus_tag="SCK51_002634"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEG1253199.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2770..4716
                     /locus_tag="SCK51_002635"
     CDS             2770..4716
                     /locus_tag="SCK51_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEG1253200.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4980..5852
                     /locus_tag="SCK51_002636"
     CDS             4980..5852
                     /locus_tag="SCK51_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1253201.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            6148..>6376
                     /locus_tag="SCK51_002637"
     CDS             6148..>6376
                     /locus_tag="SCK51_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1253202.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALECFEQYKENKRRCGRTEIIFPAEE"
BASE COUNT         2041 a         1102 c         1129 g         2104 t
ORIGIN      
        1 acagttgtgt aaaaatatca ttttagagca tgaaaaaagg agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacaag ttgacaactc ctttttgttt acaaatcaat
      121 tggatatcta caaattgtat tttctaacga attttataga cattaaaaaa ttaatttaac
      181 gttaaatact tcctccagaa agtattgaga ttggtaaaat ataatcctta tttgtctttt
      241 cgcctttaat ttttttcaga agaatatcaa caataagact tgcaatttca tcaattggtt
      301 gtcttattgt tgtcaattgt gggaaaaatt tctcaataaa agaggtacca tcgtatccaa
      361 taatttttaa atcttctggg atattaagtt taagttgatg agcaattttc atagtgagaa
      421 tggctgttaa atcatcagaa acaaaaatac catctggttt gtgatttata atagttgact
      481 ttatttccat ttcacgacgg atagtagaca gattattagg aattttgaaa atttttccat
      541 ttggattttg aaaagaaaaa cctagtgcac gtaattctgt gggagaatcc gtgttatcat
      601 ttcctgtaat cataatggta ttttgacaac catttttttg taaagtctta gctgcaagtt
      661 ttcccccttc aaagttatct gaagaaacaa tggaaacatt aggagctaaa tttctatcaa
      721 aagcaacaat aggtgctctc acacgctcat aatcagcaat tccaagatta tggcttgatg
      781 agattatgcc atcaacttgg ttggcttcaa gcatttcaat gtattctctc tctttagcag
      841 gatcattttg actattgcaa ataatcgttt tatatccatg tttaaataac tctatttcga
      901 gatactcaat gagttcagag taaaagatat tactaatatt aggaaaaata agaccaataa
      961 gctgaggtga ctttccttga agtccacgtg ctaaattatt tggtttataa ccaagagttt
     1021 tcatagcttc atgaactttt tgttttgttt tttctgataa atatccctta ttattaatga
     1081 cgcgtgaaac ggttgttggg ctaactccag ctaattcagc gacatcagtt aattttgcta
     1141 ccattttaat attttaattc aaagtatttt ccagtaggtt cgcctgattt gatttgaata
     1201 ccattttggt gattttcagg gaatacacga ctagtaaaca ctttttctcc tttattaata
     1261 aaaatctcaa tgattgagtt atctacaaaa atgttagcag ttgtttcttt agtatcaatt
     1321 gagcattcac gaatcgtacc aaagtcagtg gcatattgaa tgcctgcttt gctgcgatct
     1381 aaaataattt ttccttcttt ggtatctaca atcaagctaa gtccatttcc tttactatca
     1441 gagaaaagaa gtaactccga tttctgattt ggaggaaaag ttagttcaag ctcataagta
     1501 ttatttgttt ctgcttttgc tgtaaattgt tcagaactag cacgtaatga tgtaatagct
     1561 tctactggat attggtaaag tttaccatct tttattgaaa gttcttttac aagactcatt
     1621 gctccctgat aatcatactt atcagttgga taatcgatat caggtagtcc aatccaactg
     1681 acaatcagag cacgaccatc aggagtattg aatccttgag tcgcatacgc ttcaaatcca
     1741 taatcaagat tttgaatttc tgaagcgccc acaagtttta ctttttctat gtcaagcgat
     1801 tgacagatct taaaagtatt cggatagata ttcccataat ttagctcaga tttatccaac
     1861 ccttgaggac aataaatgag aacaggtttt ttgtctacaa atactagatt tggacactca
     1921 atcatgtatt ctgaaccagt gccgccaaaa tcaaggttgc caacttcctc ccatgtttcc
     1981 acgttattgt catccgcttt gtaaagtttg ataaagcctg atttttctaa attttgtgct
     2041 ccaacaatcg catacaattg tccttcataa ttaaacattt gtggatcacg aaaatgatca
     2101 gttacatcag caggtttatg aataagaaca ttctcacctt tcgtaatgtt gtagtcttta
     2161 tccatccatg ctccgatttg aagtgtatca cgttcccaat tttcatttcg aacgtttcct
     2221 gtatagagta agaatagatt cccctctatt tcatatgcag aaccggaata agcgccgtgg
     2281 ctatctcgtt ttgtgtctgg aagaagttct gctcctgtta catgaaaatg aactaaatca
     2341 tctgattcat tgtgtaccca ttctttcaac ccatgagcag ctccaaatgg ccaattttga
     2401 taaaaaagcg tatattttcc gttaaaaaaa gaaaaaccat taggatcgtt taacaatcct
     2461 gttttaggtt cgatatgata gctagcacgc caaggtgatc gttttacatt atccttaatt
     2521 ttatccatct catctttagt ccaatcagaa taagcacgat atcgtatttc tcgaggtaag
     2581 ttcatatttt actccttcaa tttatctttt tacaactagt atggtaaacg ttttccgttg
     2641 aaaaatcaat catttcattt aaaaaaataa actatatgac aaacgtttga catattttta
     2701 agatatgcta aaataaattt gcatttcagt gaaccgtttt cacgtaaaaa taaaaaagga
     2761 gtgattcgaa tgaattacag taaagtagct tccgacgtaa tacaagcggt cggaaaagat
     2821 aaccttattg cagctgcaca ctgcgctaca cgtcttcgtc tcgtactaaa agatgacacg
     2881 aaagtgaatc aaaaagcttt agacgaaaat ccagatgtga aaagcacatt taaaattaat
     2941 ggacaatatc aagtgattat cggtgcaggt gatgttaatt tcgtttatga cgaacttatc
     3001 aaaaaaactg gactttcgga aatttcaaca gatgatttaa aacaaattgc tagtaaagat
     3061 aaaaaattca accccattat ggcattaatt aaattattat cagatatttt tgttccaatt
     3121 atacctgccc ttgttgcagg tggtttatta atggcattac aaaacttttt aacagctgct
     3181 ggactttttg gaccaaaatc aattgaagaa atgtatcctg cgattgaagg tatttcctca
     3241 atgatccaac tgatgtccgc tgcaccattc atgttcttac caattttagt aggtatttca
     3301 gctgctaaac gttttggagc aaaccaattt cttggtgctg caattggtat gatcatgaca
     3361 acaccagatc ttggtggctc aactgaatat tggaatattt ttggttatca tgtttcacaa
     3421 acaaactatg cttatcaagt tattccagtt ttagcagctg tttggatatt atctatatta
     3481 gaaaaattct ttcataaaaa attgccatca tctattgact ttacattcac gccattatta
     3541 tcagtaatga ttactggatt tttgactttc acagttattg gccctgtaat gttattgttg
     3601 tctaatggaa ttacagacat tattgtttgg ctatataaca ctactggatt tattggaatg
     3661 ggtatttttg gaggaacata ttcacttata gtcatgactg gtttacatca atcatttcca
     3721 gctattgaaa ctcaattact ttcagcgtgg acaaatggta tcggacatgg agactttatc
     3781 tttgtagttg catctatggc aaacgttgct caaggtgctg caacatttgc catttggttc
     3841 ttaactaaaa attctaagac aaaaagtctt gcttcctctg cgggtgtctc agcacttctt
     3901 ggaattacag agcctgcctt gttcggagtt aacttaaaat accgctttcc attcttttgt
     3961 gctttaattg gttcaggaat tgctacagca gtagcaggat tacttaaagt agttgctgtt
     4021 tcacttggtt cagcaggttt cttgggattt ctttcaatta atgcaacatc tattccattt
     4081 tacgtattgt gtgaattgat tagctttgta gttgcttttg caattactta cttctacggt
     4141 aaaacaaaag ctgctggtat ctttaaagca gaagaaattg ctgaacaaac accagttaat
     4201 tcttcaaaag tagcaacaac agatccaata tctactgatg ttgaatcaac acaaacaaaa
     4261 gtttcatcag aaacaattgt tagtcccatt tctggtaaag tgattagtct ttcttcagta
     4321 aatgatccag tcttttcatc agaagctatg ggaaaaggga ttgccattaa accaaatgga
     4381 aacacggtcc attctccagt agatggtact attcaactca cgtttgaaac tggacatgct
     4441 tatggtatta aatcaaataa tggtgctgaa gttctaattc atattggtat tgatactgta
     4501 tcaatgggag gaaaaggatt tacacaaaaa gttactacta atcaaaaagt tagaaaaggc
     4561 gatattcttg gagcatttga ccgttcaatg attgagaaag aaggtcttga tgatacaaca
     4621 atgattattg tgaccaatac ttcggattat tcagaagtaa atatagtagc tgaaaataca
     4681 gtcactgagg gtactgattt acttgaaatc aaataaatat atttccctga gtaaagatgg
     4741 aattttcttt ccaaaatcaa ataatataga ttaatcaaat atttgagaat aacattttat
     4801 ttaatctttt catatatcaa ttgtatgaaa aattattaga attttaaaaa atgataaaaa
     4861 ttcaaaaaga aaaaatagta gttatttact tgtcatgtta ctaaataaaa aaataattta
     4921 aatatgatat atagtcattt atttagtgat ttcattctaa aataaaggga gaaatgtcta
     4981 tgctatacgg tgcaattgaa gcaggcggca caaaatttgt gtgtgccatt ggcgatgaag
     5041 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatgcctt
     5101 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     5161 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     5221 aattggcatg gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga
     5281 tttcttggac aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtggca
     5341 aaggattgag cagtgtcgtt tactatacga tcggtacggg tgttggcggc ggtgccttac
     5401 aagaagggcg ctttatcgaa gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc
     5461 ctcatccaaa agatgacttt gctggcagct gtccatttca tggcaactgt ttagaaggga
     5521 tggccgctgg acccgccatc gaaaaacgct tagggaaaaa aggacaagag gttgcagaag
     5581 atgatcctta ttgggaaatc gaagcctcct atattgctca atgtgcctac aatacaacct
     5641 tgatgttttc tccagatgtg atcatttttg gcggtggtgt gatgaaacaa cgccatatgc
     5701 tgcaaaacgt ccacgacgca tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt
     5761 taagcaaata tatcgtgacc ccagcattag aagacaacgc tggaacgctc ggttgtttgg
     5821 cattggctcg agaagcggtc ttacattcat aaacgaaaaa aggctcttcg acatttagtg
     5881 ttggtagaga aagatacaac tgaaaattta gtcataacca gttaggcaga gtgcttaact
     5941 ggtttttgtt ttacttggaa gatcaagcct tgaatgagat aaattcttgc tcgaaagtta
     6001 tggaaatttc gatagccgta ggccacttgt attgtgcaat agttttttac acgccgtttg
     6061 tataattaag cgagacaaat aaaaaagcca tttatgttac actcaaatcg taatacccgc
     6121 taaagcatac aaggagagtg aacataaatg acttataccc atcttacacc aaacgagctt
     6181 gtaatgatag aagcatattt tcatcaagaa actccggttg ctatcgttgc gaagcagctt
     6241 aaacgtggac gccaaacaat ttacaatgtc tataactttc tcaaatgtgg tggaacagca
     6301 cttgaatgct ttgaacaata caaagaaaat aagcgacgtt gtgggagaac cgaaatcatt
     6361 tttcctgctg aggaaa
//
LOCUS       DARBFO010000120         6300 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000120 DARBFO010000000
VERSION     DARBFO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.123"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(66..1544)
                     /locus_tag="SCK51_002638"
     CDS             complement(66..1544)
                     /locus_tag="SCK51_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1253203.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(1842..2777)
                     /gene="murB"
                     /locus_tag="SCK51_002639"
     CDS             complement(1842..2777)
                     /gene="murB"
                     /locus_tag="SCK51_002639"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEG1253204.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2850..3770)
                     /locus_tag="SCK51_002640"
     CDS             complement(2850..3770)
                     /locus_tag="SCK51_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1253205.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3883..4635
                     /gene="xth"
                     /locus_tag="SCK51_002641"
     CDS             3883..4635
                     /gene="xth"
                     /locus_tag="SCK51_002641"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEG1253206.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5499..6032
                     /locus_tag="SCK51_002642"
     CDS             5499..6032
                     /locus_tag="SCK51_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEG1253207.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1901 a         1203 c         1048 g         2148 t
ORIGIN      
        1 cttttctttg aaaaaaacat aaatcagcta ataaatatgt gagacaaagc tgccatccat
       61 aaatactagc tgattttttc agttaaatgt ctattttttt ctctgatgat ggtatttcca
      121 aaatattccc agtcatctgg agtcggtgga tttttccaaa ttatctgttc tttattaaat
      181 ttttcaagta aacaatcaga taaataaata ttcgtacgag aagtgattcg actctcaatc
      241 acaatattgt aatttttaaa tcctttcact tgagaaataa tgtaatcggt ataagcagat
      301 ccttgcgaaa aatcaataca gacatgaatc ggtgcttcaa aatatgatac cggacaaata
      361 ccgaccaata aaaagagata atcataaaat aagcgaatca tgaaacgttc atcttttccc
      421 aagaaatagc gtttattctc ttgtatgacc ttttggattg ctgtactgaa tattggatac
      481 gtctcgaaga aaaactgtag ctgcttttta gacgtaaagc tagtcatctc aaagtcgaat
      541 atccaatttt ttcgattgac atgcatcata cccatacgaa gttcttttcg tatatatagt
      601 ctttcttctt cagagatttc aatcgaaaat ggtaattgat caatcaattc cactgacatt
      661 ttttgactaa tcatgtggat atcatgaaaa tgagcttccg caagtccaaa tgcgccatct
      721 aattcaaatt cttcttccgt gatcaaaaac aacatgatat aagaaatctc atcaatgatc
      781 tgctctttag ggatatgaag aaaagaaata tgtgataaat ccttgatgat cttcattcct
      841 tctgagctct taaagtattc atctgaaaag aaacgttttc cgattggttg attcccatag
      901 gccctcaaca aactaatagt ggttagcaaa ttgaatttga ttcgttttgt tgcagacagt
      961 tttaaattga acagatattc aaaatattgc acgatactct ttgcttcatt catgacgctt
     1021 tctctaaatg gcatagaaaa accattaaag accccgaaat ataccccaaa taatacgttc
     1081 cgtatgttta tttcttcccc aactaatatg tttttcttta actttatttg atgctgttca
     1141 agaaaagaga ctaattctct tctttttaca taggctcttg atatgcttaa aaaatgattc
     1201 tctgcaaatt tttcgatcgt tacttttgaa tccacgatct ccgttaacag tcgagcaatt
     1261 tttgatcgtt gaaagtagat tcttcttaaa tttttgatat ccgtcaaatt cagacgttcg
     1321 acatggatct ctgcttcttc tgaaatttgg atctctggct ttgttttgaa ttcagtaaat
     1381 tctttattta attctatgat ataatttctt gttttaaatt tagacaaacc taatgtttca
     1441 tataacttag gcagcactac ataattcttt ttctcaaatt ctaaatatct agctagcagc
     1501 agtttttctc tatcttcctc ttctataagg taatccaact tcatatccta cacctcaaaa
     1561 aatgcctcat accaattact tgatatgcag atgaaagttc atcttttcac ctatactgat
     1621 tttatgatgg attatgcttg ttttttcatc agttattctc attttttatc ttttttttga
     1681 gaatattgca aaaaatggaa aaatttatga ggaaatattc tatatttttt gttttattgt
     1741 gttaaaatgt acaagatttg cgccatttgc gtaaacaacc acaaaaaaga gcaggacggc
     1801 caccgtcctg ctcttttact tattttaagt tccattactt attaattggc taaacttctg
     1861 cttgtttctg atattgcttg tttttctcca atgatccgca cttctgtttc taaagacaca
     1921 tcaaattttt ctttgattac ttcttggatg tgcgcaatca attcaatata atctgtcgct
     1981 gtcgcatgat cgatattgac gatgaatcca gcatgttttt cagagacttg tgcaccgccc
     2041 catttcaggc cttgcaatcc tgcttgttgg atcagctgac ctgtatagtg tcctactggg
     2101 cgcttaaaga cacttccgca tgaagggtat tctaatggct gttttgattg acgcaattcg
     2161 gtcaactcat ccattcttgc tttgattgct tcataatccc ctgtttgcaa actgaatcga
     2221 gcttccaata cgatagcacg taattcttgg actttactgt gacgataaga aaataccatt
     2281 tcgtcttttg tcaaagtttt caatgttcca tcttcaagca aaagatccac ttcagcaaaa
     2341 acatctttga tctctccgcc atacgcacct gcattcatat aaaccgctcc accgacactt
     2401 cccgggatgc cgcaagcaaa ttcaaatcca gtcaatgatt ctgccaaagc tgcataagtc
     2461 gtatcaatca gtgaggcacc tgcttccgca atgaccatcg ttcctttgac atgaatctgt
     2521 ttcatttcag tcagcatgat gactacaccg cgaatcccgc catcttgaac gatcagattg
     2581 cttgcatttc ctaataccat ccaaggtata tcatggattc ggcaataatc gatgatttgc
     2641 tttacttctt cttgtttttt cgggaatgca aggacatcag ctggtccacc tgttttcgta
     2701 aacgtatagt tcatcaatgg ttcatcaaac aatatagtaa tcgtggctaa gctttcaact
     2761 atttcttctt tattcactga ggattttcct ttcttttctc aaacgtcctc attattctat
     2821 cacgcttaat acaatcttgc cagtcttgtt caaagttgat gacggtagtg atctttaagc
     2881 acttcctcac ataattcatg acttcttaca acttcttttt gtttaagcaa ttctgtttcc
     2941 gatttaccta aacggatctg agaaaagaag gcctctacca ttggctcaaa cccgcgtttg
     3001 actagcgtgt ttgtccagtc gccaaattct ttcacttttt ttccatcagc ctcttgaatg
     3061 acaagctggg tcagatcgtt tacttcataa gtaccatttt tacttgttac ttgatatttt
     3121 tctatattag cgccacttgc taaatccatc gttaaaatag ccgtttgatt ttgtgtttct
     3181 aattgtaaaa tggcatattc caaaaattcc tttgtttctc ttatctggta ttttgttctc
     3241 aaaatgggtt catctaataa atacacagct gtatcaataa gatgtaaaaa caaatcataa
     3301 aggacgaact ctgtcgtttc ttttgccgct attcgatttt tttgcaggtg gatcaaccgc
     3361 ttatctggga tacgtttcaa ttcttctacc ataggagcaa agcgccggtt gaaaccgatc
     3421 atcaaaatta tttggttctt atcagctaat tcttgcaatt cacgaacttc atttagatgg
     3481 acactcagtg gtttgtcaat ataaacatgg ataccatttt ctagacattt tttggctaat
     3541 tcataatgta cttttgttgc tgcgtggatc atacatgctt cgattttttc ttctaaaagc
     3601 tcatcgatcg tttcatatag atgttggaac ccgtactctt tttgaattct ttcttttgtt
     3661 tctttatttc ttgttgcaaa ataaaaatca agtgttcctt gttttttcgc ataagttggt
     3721 aaatatgctt tttctgcgat attccctaca ccgattacac caatcttcat ctactttcct
     3781 cctggatcat ttagttactt tcatcttaca ccatttttta ctaaaatgag gtacaatagt
     3841 atcgcaaagg taaggaaatt aattcgaagg agtgtgcttt tattgaaatt tatctcttgg
     3901 aacgtaaacg ggcttcgtgc catcgtaaat aaaaattttt tagaagtttt tcatgagtta
     3961 gacgccgatt ttttctgtct gcaggaaacg aaactccaag caggacaaat tgatttagat
     4021 cttccaggtt attatcaata ttggaattac gcggaacgaa aaggctattc cggcacagcg
     4081 atttttgcta aaaaaccagc attgaatgcc acttacggaa tgggtatcga tatccatgat
     4141 acagaaggcc gtctgattac tttggaatat tccgatttct ttcttgtaac ctgttacacg
     4201 ccaaattctc aaagcgaatt aaaacgttta gattaccgat tagaatggga agaagccttt
     4261 tataattatt tggaaaatct aaaaaaacaa aaaccagtca tcgtgtgtgg ggatctaaat
     4321 gtcgctcatc aaaagattga tttgaaaaac tggaaaacga atcaaaaaaa tgctggattc
     4381 actccagaag aaagagcggc tctttctcga ctgttggata acgggtttat cgatacattc
     4441 cgttattttt atccaaccca ggaaggtgtc tactcttggt ggaactaccg attcaattca
     4501 aggaaaaaca atgctggttg gcggatcgat tactttttga cgagcaaaga tttagagcca
     4561 ggactagctg atgcaaagat ccatacggac attatgggca gtgatcattg tccagtagaa
     4621 ttagatttga aataaagagg ttgtgaaaaa gtcgtttagt ttcgagtatt aagaaaaatt
     4681 ttgtgaaaat ggctttccac attttgcata aatttggcat aataccgaga aactggcttt
     4741 tgaacactgt ttatcgagag attgtgaaaa agctgtcctg catcaagtaa taagaacagc
     4801 caaacaaaaa tagttcctca tatttttcgt ttggttgggc ttattaccgc agatacaagc
     4861 ttttgaacac cgtttattaa gaggttgtga aaaagctgca ctgcatcaag taataagaac
     4921 agccaaacaa aaatagttcc tcatattttt cgtttggttg ggcttattac cgcagatgca
     4981 agcttttgaa caccgtttat taagaggttg tgaaaaagct gcactgcatc aagtaataag
     5041 aacagccaaa caaaaatagt tcctcatatt tttcgtttgg ttggacttat taccgcagat
     5101 gcaagctttt gaacaccgtt tattgagaag ttgtgaaatc agcgtcctga cctgagtatt
     5161 agagaaacaa atggcaaaat agttcctcat attttgctat tttttgagct aataccgagg
     5221 gtcagctgat tgaacaccgt ttagtggaga agttgtgaaa aagtcgccta ctttcgagtc
     5281 gtaagaaatt tttttgaaaa tggttcctca caactttgca taaatttgac ttaataccga
     5341 gaagtcatga tccccatcta actccaaatc acaaggaatg agttcaaaaa ctagcttttc
     5401 tcaacaaata ttacataaaa aattctatgt tttttgttaa atttatttgc ttttttcaaa
     5461 agtatgttac agtaatttcc caaggaggtt ttacagaaat gaattttgtt gccatggatt
     5521 ttgaaactgc caatcatcag ccttatagtg cttgttctct tgcgctagtc atggtaaaaa
     5581 atagccagat cgttgatgaa ttctatacat tgatacagcc tgagactcct tttttctggc
     5641 gaaatgtcca aattcacgga atccatcaag aagacgtacg caatgcgccg aaattcccag
     5701 aagtctggca aactatccaa ccatattttc aaaaaaatcg tttagtcgtt gcacataatg
     5761 ctgcttttga tacaaaggtc ttagcgggat gcttagatta ttatcatctt gaacagccta
     5821 attatctttc tttatgtaca gtgaaaagta gccgtcgcct gtttcctgaa tttccaaatc
     5881 atcgattgaa cactgtgtgc gaaaatttga acatccaatt agatcatcac catgatgcat
     5941 tggaagatag tcgtgcatgt gcggaaatcc tgctttatca agaaaaaaaa ttcgggacag
     6001 atccattgaa aaaacttgtg actataaagt aaaagaatac ttatgaaaca cacaaagagg
     6061 tcgtgagcct gccgtttcac tccgagtcac aaggagcaat ttcaaaaaat agcttctcat
     6121 gtttattaaa tattgtgact ggtgaccgag gagctggcag gagaacaccg tttattggag
     6181 aggttgtgaa aaagctgttc tgcatcaagt aataagaaca gccaaacaaa aatagcttct
     6241 catatttttc atttggttgg acttattacc gcagatgcaa gcttttggac accgtttacc
//
LOCUS       DARBFO010000121         6287 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000121 DARBFO010000000
VERSION     DARBFO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.124"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..177
                     /locus_tag="SCK51_002643"
     CDS             <1..177
                     /locus_tag="SCK51_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253208.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="SCK51_002644"
     CDS             190..690
                     /locus_tag="SCK51_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG1253209.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="SCK51_002645"
     CDS             787..2634
                     /locus_tag="SCK51_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEG1253210.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="SCK51_002646"
     CDS             2637..3533
                     /locus_tag="SCK51_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1253211.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="SCK51_002647"
     CDS             3583..3972
                     /locus_tag="SCK51_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1253212.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="SCK51_002648"
     CDS             3959..>6287
                     /locus_tag="SCK51_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1253213.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DARBFO010000122         6267 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000122 DARBFO010000000
VERSION     DARBFO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.125"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..361
                     /locus_tag="SCK51_002649"
     CDS             <1..361
                     /locus_tag="SCK51_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG1253214.1"
                     /translation="NQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            377..547
                     /locus_tag="SCK51_002650"
     CDS             377..547
                     /locus_tag="SCK51_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253215.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            997..1530
                     /locus_tag="SCK51_002651"
     CDS             997..1530
                     /locus_tag="SCK51_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253216.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            1542..1745
                     /locus_tag="SCK51_002652"
     CDS             1542..1745
                     /locus_tag="SCK51_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253217.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
     gene            2067..2447
                     /locus_tag="SCK51_002653"
     CDS             2067..2447
                     /locus_tag="SCK51_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEG1253218.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            2429..3343
                     /locus_tag="SCK51_002654"
     CDS             2429..3343
                     /locus_tag="SCK51_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEG1253219.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            3303..3581
                     /locus_tag="SCK51_002655"
     CDS             3303..3581
                     /locus_tag="SCK51_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253220.1"
                     /translation="MNEEQTTNKNYRSKTLKDIENEIKRQRAFLAPYTQERIQTTMDS
                     QVKKLHQALLDQRNQEQKKALIQRSMDILAPLCCLVLTLLAFWLVWGE"
     gene            3588..4277
                     /locus_tag="SCK51_002656"
     CDS             3588..4277
                     /locus_tag="SCK51_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253221.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(4398..4484)
                     /locus_tag="SCK51_002657"
     CDS             complement(4398..4484)
                     /locus_tag="SCK51_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG1253222.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            5160..6110
                     /locus_tag="SCK51_002658"
     CDS             5160..6110
                     /locus_tag="SCK51_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HEG1253223.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            6131..>6267
                     /locus_tag="SCK51_002659"
     CDS             6131..>6267
                     /locus_tag="SCK51_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEG1253224.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     V"
BASE COUNT         2316 a         1094 c         1164 g         1693 t
ORIGIN      
        1 caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata aaaatgttaa
       61 tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg aagaaaatga
      121 atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac aaaaactact
      181 cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac tgcaagaaaa
      241 actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc tttctgaagc
      301 aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac tttggaaata
      361 actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt tactaggaat
      421 tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac atggtgaatt
      481 gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa aagaaacaga
      541 taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga gtgtttattc
      601 ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt attaaaaata
      661 acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa gcaaccgcag
      721 aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa agtgagcgcc
      781 tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca agaatttaat
      841 ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat aaattatctt
      901 tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac aaagaaaacg
      961 ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac tttaggggta
     1021 gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta tgcttcagaa
     1081 aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt tattgagtac
     1141 actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga agattttgca
     1201 aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa ggaatataca
     1261 aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt ttgggcagga
     1321 gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc tttgttaaca
     1381 ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa agaatgggac
     1441 aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc agctggaaga
     1501 gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg atatctaaat
     1561 cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact attcgtattt
     1621 ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg caattataca
     1681 aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag tctaaaaaaa
     1741 aataaatcga tacattttag aagaataggg ttgtttgaat attacaaagc aacaatttca
     1801 ccttcggtga aataagcgaa actttacaaa aagtggctag caaatgctag cctttttttg
     1861 tattgtggag cgtggatttt gagggggatt caagggggag cttgcgctcc tttgacaagc
     1921 ggttatttgc accaaatacg gaggtatttg gctagcaaat atagagcttg ccaaaccctt
     1981 ttagtgagct ttactcattg taccttttta ggtacaatgg tattgcgaac gatacgtccg
     2041 taggacgtac tgaaagggtg ggacttatga gcgaacaaca tcaaaatttg gctagcgatc
     2101 attctaaaaa atatacctat cgatccgagc caaaacaaat cagttttcga gtgagcgaat
     2161 ccgaatttgc aaagctgaag cagtcagctg aagctttgca aatgagtgtg ccggctttcg
     2221 tgaaagccaa ggcacaaaac gcgcgtctgg tgacaccgaa agtggcgcca gacattgccc
     2281 aggcaatcgc gcgcgattta gccaaagctg gagggaatat caatcaaatt gccaagtggt
     2341 gcaatacaca ccaacacgac gttgctcctg atgatgctca acgtttatcc gaaaacctaa
     2401 aaatcatgca aaaggagcta caaaagatat ggcaacagtt aaagtaagtc gaaccacttc
     2461 atgtagccga gcaatcaatt acgcagaacc acgagcaacc gtcaaaacag gaataaactg
     2521 tgacattgat tatgccaaaa gtgaaatgaa acaaatacgc acgctttatg gaaaagacga
     2581 tcgtgtacaa gcacacttat tgattcagtc ctttcgacca ggagaaatta ccgctgaaaa
     2641 agccaatcaa ctaggggcag agtatgcgaa aaaaatagcc cctaaccacc aaatcgcgat
     2701 ttacacacat acagacaaag accatattca taatcacatt gtcattaact ccgttaatct
     2761 tgaaactggg aaaaaatttc aagcccatgg gcaagcattt ttaaacaaat gttatgacct
     2821 aaatgacgag atttgtcttg aacatgactt aacaatcacg gaacgaggaa agaaaaccga
     2881 aaaacgaacc atgtctgaaa ttaaattaaa agaaaaaaat gaaccggttt ggaaagacga
     2941 aattcggttt gcgattgatc aaacaatgaa aaacaaaaaa actcgaacgt atacccaatt
     3001 ttgtgattca cttaaaacat tcggcattca ttgcttgaat cgcggaaaaa attttactta
     3061 cgaattggtc cacaaaaaga aaaaagtacg atccaacaaa ctaggaaagg attacgaaaa
     3121 ggagacgatt ttacatgagc tggacagacg agaacgaacc tacaatcaac aacgaaccaa
     3181 cgagctacga agagctcgag ctagcattcg cgaaactgca gaagaagtac gaagagaaca
     3241 acgaactttt acgcctgaac aatcggaaat tagaccgact cgaagaaacc atgaacaaca
     3301 cgttgaacga ggaacagacc accaacaaga attatcgctc taaaacgtta aaagacatcg
     3361 aaaacgaaat aaagagacaa cgtgcctttc ttgcgcctta tacccaagaa aggatacaaa
     3421 ctacgatgga ttcacaagtc aaaaaactcc accaagcgct attagaccag cgaaaccaag
     3481 aacaaaaaaa ggctttaatt caacgatcga tggatattct tgccccctta tgttgcttag
     3541 tactaacatt attagccttt tggttggtat ggggagaata aacagacatg gcattctatt
     3601 taccctatct actcatttct gccagtattt taggaattat ttggcttagc tataaggctt
     3661 atcaaacaag atattcatta aaaggctcta aaattcgatt taagcgattt ttattggtcg
     3721 gggttatatt tgccttatca atcgttttaa gtggtttatt cggcgtttta gagggtaata
     3781 aacgtgtttc aagaagcatt ttattagggg atatcactca aaagtatgaa gcagcatgtg
     3841 ataaaaagaa aaaagagcaa gcattagcac aaaaaatgga ggaatttacc gcttgttatg
     3901 aggacatgaa caatattttt gtggatcaag agaaacgttt aacagataaa aacatgaaaa
     3961 aactcactcg tttttatcaa aacttaccag aaaaacagca aaaagaggta caagaaaaat
     4021 atgagcaagt aaaaaaagac gtgcaatatt ttaaagatac gcagacagaa gaagcttgtt
     4081 gtaacttatt tagcgataca atcccttttt caaccagtga acaagaacga aaagaacggc
     4141 aacaatcggt cacatacgaa cgttatgaaa cactgcttca gcaagcaaca aacatacaaa
     4201 atccaacgaa aaaagaaacg gcattaaatt atctagaaag tgtcaaggaa tggttggatc
     4261 agcaacaaca aaattgaatt tgattagcaa tcatgttaaa ctgaacctgc aaatgatcaa
     4321 acattagata cacgaaagcc ccagagaggt cggattctct ggggcttttt ttcaggttga
     4381 tgtcgccttt attgatttta cttcttgttt cggttattca accaataact gaataaagag
     4441 acacaaactt ctacaagaag ggggcaaatg aaccgttcaa acattagata cacctccttc
     4501 cagtcgcaag actgtcggat aggcgacaag ttattatacc ttacaaaata aaatctgtgt
     4561 ggtgttttta agtatccctt attttcgggt tgttagggct tgccctgacc gcctgtaagg
     4621 cgctttaaga acaagataag gttttttacc caagactgtc ttaaaacgac ttagacgagc
     4681 tatcagaggc ttcccccacc tccgttactg acttgttttt agcaaaggag cctttaggcg
     4741 actgcgcagt acaatgtgag agcatagcga tcacactttt ttagcgaccg tagggagcaa
     4801 ggttttgaat tggcagtttt gaaaaatgcg cgaacctgtt ggtagaaaaa agaagtggtt
     4861 tttaggcggg aaattttgtc accctctttt ataatctctt tttaaacctt tttttaaacc
     4921 tctttaccgt cggctgtgtc cttactcctt caatggatac agcgttttat aaactacatt
     4981 ttgtctgctt ttaaactaca ttttgtctgc ttttaaacta cattttgtct gcttttaaac
     5041 tacattttgt ctgcttttaa actctttttt tgcactcacg tatgttataa tatgcattat
     5101 cacagacaaa acgttgttta taacgtctgt atccattgaa ggagtaagga ggaatatctt
     5161 tggcaaatga aatcgtgaaa taccatcatg aattaaatac cattccgttg cggaaattta
     5221 caccagttga aatgaatcta tttttctcta ttgtgtctag aatgcgcgaa aaaggcgata
     5281 aaacagtccg tcttactttt gaccaactaa aagatttaag taattataaa gcaactgcta
     5341 ataatcgttt cattaatgat ttagaaatga cttatgataa attaatggat ttacgttttg
     5401 gtaaaagaag tgctaatggg ctcgatatag atagattcgt tatgtttagt gagtttaaaa
     5461 tacgtggaac tgctaaagat ccatatgtag acatacaagt ttacgaaaaa gctttacctt
     5521 tactaaacaa tttagatgag tgggttcgct attcactcca acaatttaac gaattacaaa
     5581 gtagctatgc aaagactatg tttcgattac taaaacaata tcgaactaaa ggttttgctt
     5641 atttttctaa agaggatttt catgaactat tagatattcc aaaaagttac tggaacaacc
     5701 aagcaaacgt tgataatcgt gttataaagc ctatacgaca ggaattaacc gcaatcttca
     5761 aaggattatc aatcaaaaag aaatacggaa aagggcgagg aaaacctgtc gttggctatc
     5821 aatttacgtt taaaccagaa atcaaaaaag cagatgattt taataaacat gcaccaacca
     5881 aagaagaaca aaataaagca ttggctttag ctgataaaca aaaagaatac gaagaacctg
     5941 ctacaacaat taacgaaaaa atcaaacaga agaaacgaga acttgaactc ctccaacaaa
     6001 tcaaacaact agaaaaagag aaagaagctc ttgaaaaaca aacagagtcc gcactttcga
     6061 aaaaagagaa aaaaagttta ttatctgatt tagataattt attccagtag caacctagga
     6121 ggcaaccagt atgcaaccac aagaaacctt tacaataaag gaattgtcag aactttttaa
     6181 gatgagtagg caagcaatat cgaaacatat ccaaaaatta gattcttcca tgatagctaa
     6241 aaatgagcgt ggttataagg ttgtatt
//
LOCUS       DARBFO010000123         6173 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000123 DARBFO010000000
VERSION     DARBFO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.126"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            725..811
                     /locus_tag="SCK51_002660"
     CDS             725..811
                     /locus_tag="SCK51_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG1253225.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="SCK51_002661"
     CDS             complement(932..1621)
                     /locus_tag="SCK51_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253226.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="SCK51_002662"
     CDS             complement(1628..1906)
                     /locus_tag="SCK51_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253227.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="SCK51_002663"
     CDS             complement(1866..2780)
                     /locus_tag="SCK51_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEG1253228.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="SCK51_002664"
     CDS             complement(2762..3142)
                     /locus_tag="SCK51_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEG1253229.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="SCK51_002665"
     CDS             complement(3592..3879)
                     /locus_tag="SCK51_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG1253230.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="SCK51_002666"
     CDS             complement(3899..4123)
                     /locus_tag="SCK51_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HEG1253231.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4781)
                     /locus_tag="SCK51_002667"
     CDS             complement(4578..4781)
                     /locus_tag="SCK51_002667"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253232.1"
                     /translation="MAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKGNKKG
                     ELKLNPNYYNSQNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="SCK51_002668"
     CDS             complement(4763..5296)
                     /locus_tag="SCK51_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG1253233.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="SCK51_002669"
     CDS             complement(5317..6159)
                     /locus_tag="SCK51_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1253234.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1152 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DARBFO010000124         6002 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000124 DARBFO010000000
VERSION     DARBFO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.127"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..395
                     /locus_tag="SCK51_002670"
     CDS             <1..395
                     /locus_tag="SCK51_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253235.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="SCK51_002671"
     CDS             398..1228
                     /locus_tag="SCK51_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEG1253236.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="SCK51_002672"
     CDS             1244..2095
                     /locus_tag="SCK51_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1253237.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="SCK51_002673"
     CDS             2092..2451
                     /locus_tag="SCK51_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HEG1253238.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="SCK51_002674"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="SCK51_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="SCK51_002675"
     CDS             2624..2929
                     /locus_tag="SCK51_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253239.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="SCK51_002676"
     CDS             2929..3240
                     /locus_tag="SCK51_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEG1253240.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="SCK51_002677"
     CDS             3237..3410
                     /locus_tag="SCK51_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253241.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="SCK51_002678"
     CDS             3407..3823
                     /locus_tag="SCK51_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEG1253242.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="SCK51_002679"
     CDS             3823..4491
                     /locus_tag="SCK51_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEG1253243.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="SCK51_002680"
     CDS             4488..4865
                     /locus_tag="SCK51_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253244.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="SCK51_002681"
     CDS             4862..5161
                     /locus_tag="SCK51_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253245.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="SCK51_002682"
     CDS             5151..5309
                     /locus_tag="SCK51_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253246.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="SCK51_002683"
     CDS             5403..5819
                     /locus_tag="SCK51_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEG1253247.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DARBFO010000125         5809 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000125 DARBFO010000000
VERSION     DARBFO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.129"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            450..1604
                     /locus_tag="SCK51_002684"
     CDS             450..1604
                     /locus_tag="SCK51_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HEG1253248.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="SCK51_002685"
     CDS             1598..2020
                     /locus_tag="SCK51_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253249.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="SCK51_002686"
     CDS             3143..4075
                     /locus_tag="SCK51_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1253250.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="SCK51_002687"
     CDS             4092..4607
                     /locus_tag="SCK51_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG1253251.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="SCK51_002688"
     CDS             4903..5184
                     /locus_tag="SCK51_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253252.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="SCK51_002689"
     CDS             5186..5602
                     /locus_tag="SCK51_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253253.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DARBFO010000126         5790 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000126 DARBFO010000000
VERSION     DARBFO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.130"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(99..1229)
                     /locus_tag="SCK51_002690"
     CDS             complement(99..1229)
                     /locus_tag="SCK51_002690"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEG1253254.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="SCK51_002691"
     CDS             complement(1877..3079)
                     /locus_tag="SCK51_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1253255.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="SCK51_002692"
     CDS             complement(3139..3342)
                     /locus_tag="SCK51_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEG1253256.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="SCK51_002693"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="SCK51_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="SCK51_002694"
     CDS             4879..5691
                     /locus_tag="SCK51_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1253257.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DARBFO010000127         5615 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000127 DARBFO010000000
VERSION     DARBFO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.131"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(60..2009)
                     /locus_tag="SCK51_002695"
     CDS             complement(60..2009)
                     /locus_tag="SCK51_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEG1253258.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2051..3109)
                     /locus_tag="SCK51_002696"
     CDS             complement(2051..3109)
                     /locus_tag="SCK51_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HEG1253259.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3228..3683)
                     /locus_tag="SCK51_002697"
     CDS             complement(3228..3683)
                     /locus_tag="SCK51_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253260.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4267..5070)
                     /locus_tag="SCK51_002698"
     CDS             complement(4267..5070)
                     /locus_tag="SCK51_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1253261.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2077 t
ORIGIN      
        1 tcttacgacc catcgtttta ttctaaggtt ttgtagtaaa tatttcttga atttcatagc
       61 tacgcccctc caatcgaaaa tacaatcttg ttttctttac tttcagcaac ggaaggcttt
      121 gcaataattt cagcattaga atcacctttt ccttcaaacg ctccggcttc actcaactta
      181 acatcgaata tttttgcgtt tggataagta attattgctt cagtaatcgc ttcttcaaac
      241 tcagattcaa ctaaatgcaa gatattcctt gcaccccctg atttagaatc ctcttgtgaa
      301 gccatgtcaa aaactaagtg catggagaga tcgtctgcat aaaaattttg accatatact
      361 ctttctttcc taggtggaag acgtaaatct atatttaaat gttttcgtac taattcagca
      421 ctcttcaaca tttcattata agcaatttcc agaagagttt gttcggataa agcccaaaat
      481 ggaactattc gtccaaatcg gttaataact tcaggcttta gaccttcacc aatcaaggct
      541 tgtttgatcc ctgatgcgac ttttcggtat tctcttttta gttcagtgct gtattcatta
      601 gaattttcct tgtttgtaac acgagatagt gagctatatc ggtttaacga atcgaacatc
      661 tccgcacctg catttgttgt cgcaaataaa attgtatttg acaatgaaac ggtgtcgtga
      721 acttctcgat catcccatcc tctttcagta aaacttatcc taccttcatc taaaattgat
      781 aaaaaggcat cgagtgtttc tacaccagct ttctcatatt catcacacac aataatcgca
      841 gaaggatcat gccttacatg agtacctata agtcgtctaa aacgcattgc atcatctttg
      901 gttttgtagt ccggcatatt aactaagaga actttatttt catcgccata aagtcctctt
      961 gccacggctt tagccattgc agttttaccg acccctgttg atcctgtaaa cagagcagtt
     1021 gcttttggtt gctttcgacc tttaaaacga tcaaatccaa gcttcatacg tttaaacaac
     1081 attgttatag tataaactgc taacggttgt ccttttatgg actttaattc ctcttgaagt
     1141 ttttccatat caaaatcaga atctagctga atattatact taccaaatat ttgcaataga
     1201 ctttctcgat tataagcatt tccgtctact cgatcttttg caatcaaatc ctccattaca
     1261 gcaattgatt tagccggttc ggacaaatcc tcacgataaa gtttgtttgc ttgtacgatt
     1321 tcttgaagta attcatcagg aatcctttgc gataatctaa aagaagcctg aggatcatct
     1381 ttaacagagg atgtatttag ccattgtctc aaaatatcga cgacaacatc gctttttagt
     1441 tcattcaatt ctaacggttg tagccgtctc cctaaggcat catctctttt gatatagttt
     1501 tcatattctt ttcttgtagt agcggtaatc atttgaagtg attcatttgc taacggttct
     1561 tttagcgaat cacccccaat tttattgcca ctcccaaaag agctaacaac tttatgaaac
     1621 tcgtcaatga agaggacaaa ttcattatct ttgtcctcag cttttaaaat tcgctcatat
     1681 tctttcaatc ttggaattaa tgtttctaaa cgtgtttgga ggacatcatc tcccccaaca
     1741 cgcaattgtc cgattgataa tttatatact tgtactttct ttccatcttg tcgctgtctt
     1801 tcagcccatt cacttgcaag tgctgtttta cctgttccgg cttcacctaa caagatagct
     1861 tgcggtgttt ccggtctatt caagatcagt gacaactcat ggagttcgtc atcacgtccc
     1921 actaacttat gttttggtgg tttcattggt gtgagagcat gattaaattc ggtttgaata
     1981 ttcttaaaaa actcggtact attttccatc tgtttgtgtt cacttcctct aaaataagaa
     2041 taatttactt ctagtcagtc aaagttaggt acttaaatgt cgtcttcatg tctccaaaag
     2101 aatctttgtt aaagtcagca tttttgtatt gatcaatcaa tttttgtgta tcattgattg
     2161 ctttaatttc ggtctccatt cgactgtcta gttgacgaat actttcttgc gaatctttat
     2221 cgtcaggatt ttgttgtaaa cgttccagat actcagcttt tgtggcttca attttcttga
     2281 tgttagtatt tgaagcattt aactgatctt ctagcttatt tacaacatta ttcttacttg
     2341 taactgctac aacttttgaa taatcaatat cgccatcttt ggtaagaaag cttccttgat
     2401 agacattagt tgaatctgaa tatggattta ttctgaaatt gatattatca ggagtattaa
     2461 caggttttgc ttttttccca aaagaaatta ctccttcatt attaacatca ttcatagaat
     2521 attgagaaag tgcttttgta actgaatctg cttcattcac attgactttg gtatcatcgt
     2581 cagctgtatc atcaccagaa attagtgata cttggtttgc aataaaaact tgataagctc
     2641 tttcctctaa tttacctttt aggaataaga aagcatcccc atcagtactt aacatcccat
     2701 agctcatttc aagcttctta ggacgatctt tttcttttgt tgccatatat aagttatagt
     2761 tttttccttg cgtagataac aactttctag cgttttggga gtaaccaagt tttactactg
     2821 ttacatctct attttgatct gtccacacat ctgaaatagt gatttctgct cctgatcgag
     2881 caaactccaa tgaagaccct ataggtgttg tattgttcac gagataataa ttatagtttc
     2941 ctaattgata aatgtagcta cccaagcctg ttaataaaaa aagcactcct agaaaagttg
     3001 caagcataat gcgtaatccg tgacgtgttc tcttatcctt atcaccaagc aaccctccaa
     3061 taatactgta ttgatctttg gtagatgttg aattgacgtt tttttccatc agtttgtctg
     3121 ccccctttca taaaagagca aacaatcaga cgttttttcg gtaattctag gttcgggatt
     3181 ttgaatcatt tcggtatatt cttccccatt aatatttaac acaaaaatta tcttcctttc
     3241 ttcgttcctt tcttttgctc tttattttcc aaataatcaa tgatttttgc tattgtacca
     3301 agatagtaat tcccctcagc tttgtttttt cggattttgc taaccattgg agtaggtaca
     3361 atctcttcat ctctcaattc actgaaagat tttttaccta gatattttcc aataacagat
     3421 tttaaaaaac gttcaaaatc tttctcgtaa gttgttttct tgccttttag ttcatggttc
     3481 atattgtcaa cgacttttgc ggtttcaata attgttttag ttctcagctt aataatttca
     3541 tctggaattt tcaatcctcg taatttcatc gttgtactaa ctggtaaccc atgctttact
     3601 gcatcagcaa tactaaaact ttttagatca tctagtaagc tctccatata gttaacaaat
     3661 tcatgccaat taatagcatt cattgtctca ccccttcatt tttttaaaat gaggttcggg
     3721 cgtaacacat ttatgttatt ataatatcat aataacataa ataaatcaat cagcaaattt
     3781 agatatccct taaagtatca gcaattattc aacaagatat ttattttagc tatttcagtc
     3841 atcataaaag actaattagc aataaaataa ctaatattat ccatttatca gacgaaaaga
     3901 aaaaacttat ctaattactc tatattaact tttaacaaat agttgacttg tccaaaaatg
     3961 ttaccttcaa tctcacaaag atattaagat gtttcctacc aaaactgata tcaccggact
     4021 ttttattctg tgttgtccaa aagtgttacc tttagtcgat caacaaatta aaaaaaattg
     4081 tttttttctt gacttgtcca aaagtgttac ccatagttgt taaaatttgt atagattagc
     4141 cattctttcg aatattattt agactgaaac ttgcttacaa aaaaacttct tgcatttatt
     4201 gtccaaaagt gttacccata cttttttaaa gaagcaggtt gtattaaaat tacaacctgc
     4261 ttcttctcaa tcttcttcta accaattatg cattgaaatg gttggcaagt ttgattctgg
     4321 ctcactaaaa gtgaatctga gccgatcaac tcttcttcct ttctttgcat agattttttc
     4381 ataatcaaaa gtttcaaata cctgtattcc gttttcatca ggagctaaaa attcttcttt
     4441 cgcctgctct agtattcttt tgtcaatatt tgtcattcta taacttgaag gtatacttaa
     4501 tgctcttcta aaatcttcaa ctttgactac ccaataacca tttcgccact ctttatttct
     4561 gtattgcatg agatgacgat acaattcttt tgtgtagcta cttcttagtt ttgtaaaatt
     4621 atcaagttca aaacgagtaa agtttttgcc aattgagttt aagataaatt caaattcctc
     4681 atttgcctta attgtaactg tttccttctg tttatctatc cggaattttt gaaaaagcca
     4741 cagctgttca tagacgtctg aatcttcaaa acggtaggtt aaagtaccta gtttgtcact
     4801 caaagctttt aacacagaat aaaaactgtc cttttctctt ctgtcgtatt tgaccaaata
     4861 acgaaagagg ttgaaatcaa aagttacttc tgctgttcct tgacgtttca tttttgataa
     4921 aatagcccaa aacacatcca tttcaacagg cttaaatcct cgcaaagctg ttgtattcag
     4981 accattgtta taacgcacaa ttgcatttgt ttgttctgat actaaatcta catcactatc
     5041 aataatcttt tcggtaactt cttgagacat tgattactct ccaatcttct ttttgcgctc
     5101 ctttaatcat gttaatctgg tactaattaa aggaaacaat tttttctgct tgttacctaa
     5161 attaaatcac aactaaaggt aacagtcaac aaagatttgt tactaatcta attttctatt
     5221 ttttcgcctt ccaaatcaag aaaaccgaag gtaacgtctt atcctagtgt gatacctcaa
     5281 atgtccactt ttgttacctt tgctgtccaa aagtgttacc cttactgtcc acttttgtta
     5341 cctttactgt ccaaaagtgt tacctttata cccctttaat acttactctc ccaagagatt
     5401 aagcctcccc taacagatat attaacagat atttaacaga tatattaaca gatatttaat
     5461 aaaggaggat tactcctctc ttatttcaaa atcttaaaaa taaaaaatct taaaaaaaac
     5521 taacactatc taggagttta ttttgcttgt gtaactttga ataattgaaa aaaaggaaaa
     5581 ggaggaagct tcagcttccc cttttattta tattt
//
LOCUS       DARBFO010000128         5532 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000128 DARBFO010000000
VERSION     DARBFO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.133"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..985)
                     /locus_tag="SCK51_002699"
     CDS             complement(<1..985)
                     /locus_tag="SCK51_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HEG1253262.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR"
                     /locus_tag="SCK51_002700"
     CDS             1137..1832
                     /gene="vanR"
                     /locus_tag="SCK51_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEG1253263.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="SCK51_002701"
     CDS             1810..2964
                     /locus_tag="SCK51_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEG1253264.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="SCK51_002702"
     CDS             3179..4147
                     /locus_tag="SCK51_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEG1253265.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="SCK51_002703"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="SCK51_002703"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEG1253266.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..>5532
                     /locus_tag="SCK51_002704"
     CDS             5177..>5532
                     /locus_tag="SCK51_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HEG1253267.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRG"
BASE COUNT         1669 a         1078 c         1252 g         1533 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc gg
//
LOCUS       DARBFO010000129         5220 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000129 DARBFO010000000
VERSION     DARBFO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.134"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1256)
                     /locus_tag="SCK51_002705"
     CDS             complement(<1..1256)
                     /locus_tag="SCK51_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG1253268.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="SCK51_002706"
     CDS             complement(1270..2487)
                     /locus_tag="SCK51_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG1253269.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..4072)
                     /locus_tag="SCK51_002707"
     CDS             complement(2477..4072)
                     /locus_tag="SCK51_002707"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEG1253270.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            4197..4982
                     /locus_tag="SCK51_002708"
     CDS             4197..4982
                     /locus_tag="SCK51_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HEG1253271.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT         1574 a         1051 c          862 g         1733 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     3661 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     3721 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     3781 aaagatactc tggtgagatc gaatacccca aaatatcaac gattgtggca atcaggtctt
     3841 cttttgattc ttcatctgaa agtaattcac gatacaacat tgtttgtttg cttactgtgt
     3901 catattcctc taaggattca tctgctaaaa gaaccacttg ttccaacaaa cggtcggata
     3961 aatatttata gaaaatcagt cccaataaat aatttttata ttctgacgca tccattttac
     4021 tacggagatt atctgccgca ctaaataatt tttgatttaa ttctgcaccc aaaaagatac
     4081 actcctatct cttatttaaa gttgctatat accctcttat tttagcatac tcccttttag
     4141 aaaaagcaaa aaaaaaagaa aaaatcttat tcttgataac aagctacgtg acgtatatgc
     4201 ctaacaaaaa tagaactttt gaatcaatct taaatgaagg tttaaccaca gaaccggcat
     4261 ccattatttt tgatgaagtt atcgatgacg ttgaagatag taagtatcat aaaaatttaa
     4321 aagaactttt ttattttgca ctcaacaatg gaccaagttt gttcactggt atatcagtac
     4381 ctgatagtga taatccatta attatggcaa aaatttactt aacaaaatgg tgtaataaat
     4441 acatcaggga taggaataac ccagcattaa aaaaacctct taaaacattt ggcgaaaaag
     4501 atgctgcttt aactactaga gtagcagcaa atgctaatat tgatgatcaa aaagtattga
     4561 atgactactt gcgtggacat tttttatata tgagcgctga gaacatgaac ggaagtattt
     4621 tagaggaata tttagccgaa gtgttagaac ctgaaggttg gatttggtgc gccggctctg
     4681 tatatcgagc tgttgatttc tgttatttag gaacctcacc aattctttta caagtgaaaa
     4741 ataaatacaa tacagaaagc agttcgagtt cagctataag agtaggaaca acaattagaa
     4801 aatggaatcg tttaaataag tcgacaaaaa tttctggttt ggatagccct atccccaatt
     4861 ggaaagcatt aatagaaatg actgaagcta gtaaggaact ggcagctaag ttaactgaaa
     4921 atagttattt agcttatatt aatgaaaaat ccacacgaga attatggaca ctcgatgact
     4981 aacaatatcc ttattagtat ctatccacga tacatggaaa aaattataaa caaacaaaaa
     5041 aactttgaat ttagaaattt ctatcttacc tcagatcctt ataatatctt ttggatttac
     5101 gaaacaatcc ccaccaaagc tttaaagtat aaattgattg taaaaaatcc aattactaaa
     5161 cttaagaaaa atcaacacta ctttttagga gacgatgagt ttgaacaatt agtttaaaag
//
LOCUS       DARBFO010000130         5039 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000130 DARBFO010000000
VERSION     DARBFO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.135"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            413..619
                     /locus_tag="SCK51_002709"
     CDS             413..619
                     /locus_tag="SCK51_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEG1253272.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(821..1822)
                     /locus_tag="SCK51_002710"
     CDS             complement(821..1822)
                     /locus_tag="SCK51_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1253273.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1827..3740)
                     /locus_tag="SCK51_002711"
     CDS             complement(1827..3740)
                     /locus_tag="SCK51_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEG1253274.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3908..4414)
                     /locus_tag="SCK51_002712"
     CDS             complement(3908..4414)
                     /locus_tag="SCK51_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1253275.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4574..5014
                     /locus_tag="SCK51_002713"
     CDS             4574..5014
                     /locus_tag="SCK51_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEG1253276.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
BASE COUNT         1391 a         1008 c          769 g         1871 t
ORIGIN      
        1 tatttttgtt tcttattttc taaaaaatta aaaaaaattt tggtgaatta agtatttcct
       61 tgataacact ggcttttggc ctcattttag gttatcattt gtaattttat ttcacagttg
      121 atatgattgt tatgtaaaat aaaaaagttt aggatacatc gaatttttgg ggaaattctt
      181 ttgggggtat cctaaatctt ttctattata ttcccatatt ttctattatt ggtctagacg
      241 tatgatggta aactttgtac ctatctgatt gctgaatcat taattgctta tcattagcca
      301 tctttgtttc aagttcgcta gatgatcatt ttattcacat cctaacaaac ttgtcacatt
      361 tatagattct taatctattt atcatacaat tataaaaaag gaggtgctgc ttatgaaagc
      421 tatccatatt tctttgcttt ttttcgctag tgcattgatt ttatttgcta ttcatctttg
      481 ttcccagcct tatatcaata acaacggaat attagtggaa ccagcattct tttgcttacc
      541 actcggcttt ttgtcccttc ttgcctccgc aggcttcgga tttgttgctt tttttaaacg
      601 atcaaaacaa caaatctgac ttataaaatt tacgtaaaag agatcgtgac aataatcgtg
      661 tgatattccc aaatttcgaa taaaaggtgc cccttgccat ctctaactcc tcagtcacaa
      721 gttacaatat tcataaacca tgaaaagctt gtttattgaa attgctcctt gtgacttgga
      781 gctaagtgac agattacaac ctcttttgca tcataagact ttactccttg aaaaacatta
      841 ctaacttttg atatgtttct tgcgcttgtt tattgctgaa atcaaactga tattcatggt
      901 tcaaaacagc atttttctcc gcataatcta cttttgtgac tttcaccccc gctttctcaa
      961 atacagaagt tacttcttcc atttgtttcg taaacgtatt tgtatttcca tccgttaaga
     1021 atgtgttttg cgtccaacga tccgcatgtc tgaccaaggc taaacttttg accttctctg
     1081 attgttcaaa gtccttatta tcaaagtaag cccatccaca tttttcataa agaaagctag
     1141 agattttatc acttacttga gtaagtgcag aaggatccaa cagaccagac aaagaaataa
     1201 acttcgtaat ctgcttgttc tccaaagtcg gatcaagatg atcagctttt ttaatttctt
     1261 cattttgaag tgctaacaaa tattctccac taatttgtgc acctgccgaa tttccaccaa
     1321 tcgaaatatt cgaccaatct gcttgatctt ttatctcttt tttagctgct tgaatggctt
     1381 cgttcaattg gataactggt gtaggatgtt ttgcttccgg tgatcgttca taatctatat
     1441 ttacgatgat ttgctgcgtg tctgaacata gatattccaa ataatcacga caatcatttt
     1501 tatcccctcc tacatatgcg cctccatgca tccagaaaag aatcggtaat tttttctttt
     1561 ccactttcgg tgtgtagata tccatattat tatttttgta ctttgaagga taggatattt
     1621 ctttttttat atcgatttgt tcgatttttt gctggaaatc atctggtcgg gcatatactt
     1681 gttctttttc acttgtgtca aattgttttc taacaagcaa gctaatcggc tttggtgtga
     1741 tttgattaat caaaaataca gtgactaaaa taactacgat gataactggt actgcaatca
     1801 ctattcgctt gaaccatttc attcatttac cctctctttc ttcgccaaat ccatgtgata
     1861 atcgtgctaa cagataaaaa tccacctgaa taaatgagaa gcgaatgttt cttttctcca
     1921 gttttcggca acattctttt ttttgcatct gtcttttttt gtactttcac tgtcatctga
     1981 atcggttgcg tatcatcact tgattgatcc tctcgaggtt cattctttcc cttattgtct
     2041 gcattctctg tcggatcttg cttttttggt ggagctactg ggattttttt gtaatagaaa
     2101 ataatagttg gtatcgtctc attaaaaatc ccttcagtcg gtccttcaac atttgctaat
     2161 tcgtatcctt cgaatgtctt ttgctctgtc atataatttt ctccataagc tccgtaaagc
     2221 tggatttcgt cctggtcttt gagtagactt ttattgtttt catccacata ctttacgttt
     2281 atctgatcat ggaacactac tttcaaaaag tccgttacat gtttcgtgta ggcagcatgg
     2341 tcaacttcct gtgcctttcc atgccctgct ccttctacta ataaaagatc tttataccct
     2401 cctgataact catataattt ttgcgcgtta gacacaggta ctacatcatc tgctgttcca
     2461 tggataatca atttaggcaa tgaatctgac attccaatcg taggtagagc agcttttgta
     2521 tccaaatgca tctcctgatc aaaatagtat tgtttcaaaa ggcccataaa taaatcttca
     2581 tgctcttgat ccccaatgat gtctaactga ttttttatgg catccggtag tgcgggggct
     2641 actgtctggc tgtacgtaaa acgtagttgt tcatcaattg atgcaaatcc gcaattttca
     2701 ataattcctt tgacattgac aggaatatct ttcgctaata cagacatcat cgtagctgct
     2761 cccatcgaac caccataaag taaaatcttt tgagatggcc gctctcgaac ttcttgattt
     2821 atccagtaaa ggacgtcatc tgactcatat tgtccatagg tcacataatc gcctccgcta
     2881 ttaccagtcg ctctggaatc aacaaataat aaattatacc cggccttata aaaatcattg
     2941 tattcttctg taacgatccc gttattccaa ttccctcgaa atccgccatg gatcatgact
     3001 gttttatctg aaccttgatc gacataatat gcatatacat ccatcatttt gccatcttgt
     3061 ttgtttagta cagtttgatg aagttgtttt gtatttggat agaagcctgt gttttctgta
     3121 ggcgttgtcc catcatctgt acctccaagc atcgacatca acacactgtt ttttgcaaaa
     3181 gcgatatcgt acaatagata agtagcaatc agatgtttga taatctcttt atctacacca
     3241 taatcattaa cagcaagctc agtgactaat aactctatta attttgactt gatgtcttct
     3301 gttggtccat taagtatttc tgtcatctgt ttaccgaagt aattcaaata ttctttacca
     3361 ttgaaatttt cacctttacc gatatcatca ttttctgtcc cattctcttt gtcacttact
     3421 ttagcagaag aggattcgtc gtttgacatt tttgtagctg ttgcttcttc tgtttgttgg
     3481 ttatctgtgg tagcggtcgt ttcttgatct gtgctagtaa cagatgtctc aaccgttgtt
     3541 tcagtatttg tttctgtttg tgaagtactc gatttcgttt catttccagc atcagtagtt
     3601 tctgtggttt ctacctcccg agattccgat tcggaagaaa tgctctctcc atatactgtc
     3661 cattgcggga cgcttgtact tacagcaaat aagatgacca acgctccctt taatacttgt
     3721 tttgttctca agctattcac tcctttttat gtttctagga gaattatatg taaacgcaaa
     3781 cattttttat acaaaaaata aatggtttat cccataaaaa tagaacagga ttattttctc
     3841 attttacgta ataaagcaga atggataaac cagtatccat tctgcttttt ttaaacgaag
     3901 aaacaactta ttgctccaaa aatgtcagaa atcgtttatc ccaaattttc tcgtaaaaag
     3961 cgattgtttc ctttgctttc ataaaaggat tatcataagt tgtcgttgct cctctacaca
     4021 tcataagctg gtatcctaat ccatgagcaa acttcacagt cgtatctata caatactctg
     4081 cttgtgcccc acataattcg atcgaatgac agtccaattc agttaataaa acactcaaat
     4141 tagtcttata aaatgaattc gcatgggttt ttctcacaaa ataactgtgc tcaggaatag
     4201 ataattccga caatagctcc catggttttg tttgtggcac taattgagca tcttcgtgtt
     4261 gaacgaaaat aactggtttc ccaatcgttt cgtatgtttt gattctttta ttcactaatg
     4321 ctgtcactgt ttctaattcg tgtatcggcg aagaatcaaa acatacccct ttttgtaaat
     4381 cgatcactac tattgcgtcg gctgtgtttg acatacttgt gccttctttc ttttttcttg
     4441 atttaatagt atttcttatc tagctagttc cattatacgt gacaacattt tcttatttta
     4501 cacctctaaa tggcactttt cgactatttc ttcatgttat tatttcataa atacttagct
     4561 attggaggaa tgtatgatga ttaaattgta tacaggttct ggctgctcgt cttcaagacg
     4621 cgcaaaacgt tggcttatcg tccatgatat accgtttatc gaacagaact taattcgtca
     4681 accactaacc aaaaatgaat ttatccactt actaaaattg acaaacaacg gcgtgaccga
     4741 aatgctctct actcattcta ttagttataa aaatcttgtt cacagaatag atgaactcag
     4801 tttgagtgaa ctatatattg agattcgtac atccccttct ttattgaaaa aaccgattat
     4861 ctacgaccaa tctgcaaaac ttttgactgg atttagcgaa gataaaatca ggatgttcct
     4921 ttctaaaact aaaagaaagc aagagctcaa cgaactcttg ctttctggtc atcttgtcga
     4981 ttttaaagaa aataatcttt ttgcaatgtc ttaattttgg cataaacaat ctgcttttc
//
LOCUS       DARBFO010000131         5034 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000131 DARBFO010000000
VERSION     DARBFO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.136"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="SCK51_002714"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="SCK51_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEG1253277.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="SCK51_002715"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="SCK51_002715"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEG1253278.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="SCK51_002716"
     CDS             complement(2169..2906)
                     /locus_tag="SCK51_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEG1253279.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="SCK51_002717"
     CDS             complement(2997..3524)
                     /locus_tag="SCK51_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253280.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="SCK51_002718"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="SCK51_002718"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEG1253281.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="SCK51_002719"
     CDS             complement(4401..4886)
                     /locus_tag="SCK51_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEG1253282.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
BASE COUNT         1337 a         1125 c          862 g         1710 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acct
//
LOCUS       DARBFO010000132         4921 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000132 DARBFO010000000
VERSION     DARBFO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.137"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(99..1298)
                     /locus_tag="SCK51_002720"
     CDS             complement(99..1298)
                     /locus_tag="SCK51_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1253283.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1428..2804)
                     /locus_tag="SCK51_002721"
     CDS             complement(1428..2804)
                     /locus_tag="SCK51_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1253284.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2804..3460)
                     /locus_tag="SCK51_002722"
     CDS             complement(2804..3460)
                     /locus_tag="SCK51_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1253285.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3470..4747)
                     /locus_tag="SCK51_002723"
     CDS             complement(3470..4747)
                     /locus_tag="SCK51_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1253286.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4766..>4921)
                     /locus_tag="SCK51_002724"
     CDS             complement(4766..>4921)
                     /locus_tag="SCK51_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEG1253287.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1542 a          899 c          597 g         1883 t
ORIGIN      
        1 aatcaagcgc ggcatcaatc tttcgaacga gatgatcttg aggaactaga tcatcaatcg
       61 taagcatcgc aaattgattt ctaccattcg tattgttttt agacatcata gctatcactc
      121 cttgcattcg ttttcataaa tctattataa aagaaaagac tgtagacatc atccataaaa
      181 tggactttgt ctacagtctg cgcattctca ttagagaaca ctcccttttt tgtgactttt
      241 ctgaagctag agtgaatact ttcaactgca ttagtagtgt acatgatttt tctaatggcg
      301 ctgccataat caaagagttg ttcaacatga gaaaagtttc gtttgcatac atctactgca
      361 ccagaataat gagaccaccg attttgaaaa ctgtcgaagg cagcgtgtgc agcgtttaaa
      421 gaagaagcac tgtagaattt tttcatgtct cgacagactt ccttataatc cttacttggg
      481 aggtatcgta acgcatttcg aacaagatga acaatacagc gctgcacaat gacagaagga
      541 aaaattgctt ttgcgccttc tttgagaccg gaaacaccgt ccatcgaaat gaaaaaaaca
      601 tcttctacac cacctacttt cagttcatca aatacttgca tccagcgatt tttagattct
      661 gtttgattga gccataggcc taaaatctcc ttgtttcctt taagatcata gccaagaatg
      721 gtgtacacag catattcttt agcttcatag ttttcgcgta aagtgacgta catacaatca
      781 acgaatagaa atgcataaca cttggctaat ggccgagctt gccattcttc caattcagga
      841 aggacggcat ccgtgatatc agaaatcatt tcgtgggaaa tatcaaagcc gtaaatatct
      901 tcaacggttg cggcgatatc gcattgactc attcctcgtg catacatgga aagaacctta
      961 ccttcgatgt cggagacatc tcgttttcta ttaggaatca actcaggttc aaaagaagcc
     1021 tcccggtctc tgggaacatc aatgtttact ttaccaaagc tcgttttaag cgtcttattg
     1081 ccataaccgt ttcgtcgatt gtcatgtttc tttggctctt tagaatgggc gtcataccct
     1141 aaatggttat tcaattctcc ttgaagcatt ttttcaaata agggaccaaa cacatctttt
     1201 aaaacatctt gcatatcctc tacagattcg ggttgataag tattcaaaat ggattcagct
     1261 aacttctcag catcaggatt tcttttcttt ctagccatcg tgtaatcacc tcttgatctt
     1321 attttagaaa aataaaaacc gtgaagcaac cacccgccta ggattgatgg ttacttacac
     1381 ggtttatatt acactctcat ttttggtgct aaaaagcacc gaaaatttta attattgtca
     1441 attaataatt cttttggatt ccttcttaat atattgaatg aagaaataat caatgataca
     1501 agaagtacta aactcataaa tataactaca taaatcatga atttaggtaa tacattgata
     1561 tctaaacttg ataatgtctt gttaaatcct tcagcttctg ccccaccacc taattggcta
     1621 gatgcagatt gtttagctat ttgtttagct atatcaccag taaccttatt caatatatta
     1681 ttcccaagct tgtcagctgt atattgagct aagaaatagg aaccaacgaa tgcggggatt
     1741 gatataaata ccatctcaat taggaattga ccaaaaattt ctaattttga tatacctaat
     1801 gatagtaata ctgctatttc tttctttcta gcattcatcc ataagaataa taataatgaa
     1861 actacaactc ctgcaaatat taatgagcca aaaaataatt tatttgaaat tgagtaaata
     1921 cctgatatag attgttgtaa tgcagggtaa tttgatgaac ttttaatcaa attatattct
     1981 ctccaattta tatctaattt cccaagatct tttattacac tatcaagatt cttatcacct
     2041 tttacaaaga atgttgcatc ttggtataca gctgtatctt cggtattacc ataaacttta
     2101 gcagctgtat gaatatcagt gattaatgta ttttcgtata gttcttgtgc tgcacttact
     2161 ccaccgctat tatgcccatc gaatagacct ttaatttcta cttctactgt ttcatctgca
     2221 cctttctcat tatcagcatc aaataaatta gattttattt ttatcttatc tccaacttta
     2281 aggttatttt tttcagctaa atctttatgc attaagattt tatttttatc ttcattttct
     2341 aaatgctttc cttctactaa tttatatgct tctgatacaa attttgtttc ttttgttgag
     2401 tcgttaaccc cagttaacat aactgttctt ttaaaattct ttgctctttc aggtgattga
     2461 ttcgcaagag tctcttgagt ttcaataata tcataatcaa ctaaatctgc aacactgttt
     2521 attcttttta cataggagtc tatactatct gtttgagata tctttttaat atcttcacct
     2581 tttacatttc ccccacctct aggtgttcct ggatttactt gtctatttat ctccatagaa
     2641 aaactatttg ttatattagc aaatgtttct tttgaagctc tgtccgtagc atctttaata
     2701 gataaactaa tgagacttaa agttgacata gataaaataa ctaatatgat aattaatgat
     2761 tttaaacttt ttctagttac ataagcaaat gcatttttta tcattattca acctccaaat
     2821 tcattttatt tacttttttc aactttttac cacttagttc taaaatgata tccgcagaat
     2881 ctgctacttc cttactatgt gttacaacta ttacacattt atttctatct ttagctaatg
     2941 tctttaatat attaattatt tctccagcag taacactgtc tagattacca gtaggctcat
     3001 cagctagtat tattggagca tctgatacca atgctctagc aatagctact ctttgttgct
     3061 gtccaccaga taatttcata acatttcttt ttatttgttt tttatctaaa cctagttcaa
     3121 acaagatact ttcatctgct gatttattta ctaatctaat attttcaatt ggtgataaat
     3181 aatctattaa attatagttt tgaaatacta aagatatatt attttttcta tgattactat
     3241 atcctttatt ttgtatatct tcattcttaa acaatatttt tcctgtttga ggtttatcaa
     3301 gtccagcaag taaggaaaga agtgtagatt ttcctgttcc tgactttcct actatcgcat
     3361 aaaacttccc aagttcaaat ttttgattta ctcctgacaa aactttttct ttagaatttg
     3421 cataactata tgctacattc tttatttcta atatatccat tattttctcc taacttattt
     3481 ttgataagat ttctttaggc ttcttgatta acattaatga agaagcaaat acaactgata
     3541 aaacaataat acttattaat attaaataac tttgtccaag tgttgttata tttgacataa
     3601 aactactatt atttattaaa cttccaccgg aaatcattga atcctctgag ttaatgaatc
     3661 catctacaat tacttttagt aatacatttc ctaaaaataa ggaagatatt atacttggta
     3721 tcgatatgaa tattaactcg aatataaatt gcattataat ttgtatctta gatgttccaa
     3781 tagataagaa tatacctatt tcataaattc tttcccttaa ccatagaatc aagattaatg
     3841 aaagaacaac catcccacct aacataatag aataagtcat tacttttatt atatatttta
     3901 ttccactcac tgactctaaa gactcttcaa acgcattaga atctttttca acaaaatact
     3961 ttgactcatc aattttaagc tctttcaatt tgtttaaggc taagtctgta gattctgcac
     4021 taccagaata cattaaaatt ttatttgcaa tttcattatt ttctgaatta tttaatatct
     4081 cttggcttgt tgaataatct acaaacacca tattttcact aaaatcagaa gataatcctg
     4141 tatatgtttc ctgtttttta ccagaaaaga tccctataat tttaaattta tgacttttta
     4201 tttttccact tttttcaata tctagtaatt caagatcaac ttcatcacct aattttagat
     4261 tgttttgttt agcaaattct tcatgaacaa taattgaatt cttatcattt tcttctatat
     4321 ttttaccttc tttaattgta aatactccac tactaaataa aatatttctt ttagtattat
     4381 ttgtagcttc gagtgaaaca acattcttaa attcgtcaga taaatcttct ctatttattc
     4441 tttgttctcc gctaactact ttagcatctt ttagttttgc taatccatca tattgaatta
     4501 ttttttcttc aatttctttt aatttttcaa tatctttaaa ttgattaaca ttaaaatatt
     4561 taccatcttt ttttgttatt gatattgaag aattagaact ttcatataaa gccttttcta
     4621 tttcattgct tgatttcatt attgttaaac aagaatacaa gcaagaaaga actatagtta
     4681 atatgataaa aattattaaa gttctatttt tctttcttgt aatatatgct atagcatttt
     4741 ttatcacttt cctaactact cctctctatt ttcttactcc ataactaatt gcatccactg
     4801 gacaagcatt tttacatcgt ccacatctga tacactccag atgattgcaa ttctcaactg
     4861 aatctttatt catttggcaa acttttttac attttccaca attaatacac ttatccttat
     4921 c
//
LOCUS       DARBFO010000133         4800 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000133 DARBFO010000000
VERSION     DARBFO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.138"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..724
                     /locus_tag="SCK51_002725"
     CDS             <1..724
                     /locus_tag="SCK51_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEG1253288.1"
                     /translation="DFLSTPFDEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGL
                     PVLLSTGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPV
                     GYSDHSLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTE
                     IFLGDGVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQ
                     YIGKKVKRPISADSVLLEEDVE"
     gene            721..1869
                     /gene="neuC"
                     /locus_tag="SCK51_002726"
     CDS             721..1869
                     /gene="neuC"
                     /locus_tag="SCK51_002726"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEG1253289.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            1842..2519
                     /locus_tag="SCK51_002727"
     CDS             1842..2519
                     /locus_tag="SCK51_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEG1253290.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            2509..3651
                     /locus_tag="SCK51_002728"
     CDS             2509..3651
                     /locus_tag="SCK51_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEG1253291.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            3664..4641
                     /locus_tag="SCK51_002729"
     CDS             3664..4641
                     /locus_tag="SCK51_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1253292.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT         1759 a          605 c          938 g         1498 t
ORIGIN      
        1 tgattttttg tctactcctt ttgatgaaga aagtgcagac tttctaaaac aaattggtgt
       61 acaaggattt aaaattggct caggagattt aacaaattta ccttttctta gaaaaataga
      121 tatgtatggg ttgccagttt tattatctac aggtatggct gatattcatg aggtcagtga
      181 ggctgtagat tgtttcatag atagtccagt tactatttta cattgtactt caaattatcc
      241 ggctagttct gaagatatta atttattggc gattaacaca ttaaaagaaa tgttcgggat
      301 acccgttggg tactccgatc attcattggg atatgatgtt ggtatttgtg ctgttgcgat
      361 gggtgctaag gtaattgaga aacattttac cttggataag gaactacctg gtccagatca
      421 taaagcttct ttagatcctc aggaatttgc agattttgta aagcatatta gaaacactga
      481 aatattttta ggtgatggtg ttaagcgagc aatgccttct gaacttagta caaaagaagt
      541 atcaagaaaa agcctagtgg tcactgagaa tttaaatgtg ggagatattc ttacagagaa
      601 atctcttaca ataaaacgtc ctggaaatgg aatagctcct aagtttttat atcaatatat
      661 tggcaaaaaa gttaaaagac caataagtgc agatagtgtt ctgcttgagg aagatgttga
      721 atgaaaaaaa tacttatagt aagtggaaca cgagctgatt atggaatata taaacctgtt
      781 gctgagaatt tactacaaga taattgggat gtatcatttg ttgtttctgg aatgcattta
      841 tcccataaat atggatttac taaagaattg atcttagctg acggatttag aattagtgga
      901 gaagttcaaa gtttgttttt agagaacaat aagggaaaca tggctcggtc tgtggcatta
      961 gagataaatg gtttcacaaa tatatttgaa caaactagcc ctgattttgt attattacta
     1021 ggggatagag gagaaatgtt ggcagctgcg atagcttgtt tgtatcttgg aatcaaaaca
     1081 gcacatattc acggaggcga agtgtctggt actgtagatg aatcaataag acatgccata
     1141 acgaaaatag catctgttca ttttgctgct acggaaaaaa gcaaagagcg tttaataaaa
     1201 atgggggaag attcttggag agtttttccc gtcggagctc ctagaataga tactatttta
     1261 aataatagat tacctagttt tagagatata aaagaaaaat atcatctaaa atttgaaaag
     1321 aaaaaatacg ttttgcaagt atttcatcca gttgtaaccg aactagaaaa tatagaaaaa
     1381 caaattttag ctttgatcaa ttcaatggaa caacaatctc aaccaattat ttgcatttta
     1441 cctaatagtg atgctggtag cgaaattatc agaaaaatgt accagaaata tttaaaaacg
     1501 gatattattt atattacgaa tttagagccg gaggaatacc tgactatatt aaaagagtgc
     1561 aaatttatga tcggaaattc ttcttctggg ataattgaag ctgcatcatt caaaatacca
     1621 gtaataaatt taggaaccag acaaaatgga cgagaacaat ctaaaaatat tataaatgca
     1681 aatctaaatg aaaatgaaat tgaaactgct ttgaataaaa tacaagatcc tcagtttata
     1741 aaaagcttga aaaatgttaa gaacatctac ggtgacggca acagttctag aagaataaca
     1801 aaaacattga atcaaatatt gaatgatggg gaagatatta agtggattca aaaacaaata
     1861 gcttattaaa atatactagt gatccataca ttattattgg ggctggcgga cacgcaaaag
     1921 tcattattga aaccttaaga ttagaaaata agttaatata tggatttttt gatgataagc
     1981 aaaataaaat gcaagatctg aaatatcttg ggaaaataaa cgaattgaat agctgggcgg
     2041 atagcaaaaa aaaaatctat aaatttattg tagctattgg tgactgtgta gtaagaaaaa
     2101 agattataaa aaaattgaat atgccaagtg agcaatatgg aatagctgtt catccaagtg
     2161 ctatagtggc aaaagaaatt tcaattggtt atggaactgt tatttttgcg aatgctgtaa
     2221 ttaacccaga tgctgtaatt ggagagcatg ctataataaa cacaggtagc attgttgagc
     2281 atgattgtag aattaataac tatgttcatt tatctccagg tgtatgctta gcgggtggag
     2341 tacatgtagg tgtaggaact caaataggaa ttggttctca atgtatccag atgaagaaaa
     2401 ttggatcttg gtgtatgata ggcgcaggga gcactattgt taaggatatt ccctcacatt
     2461 ccttagctta tggcaatcca gctaaaatta agaaagaagg aattaatttt gaattctaga
     2521 attttattat caagtcctca tatgggagga acagaagaaa aatatgttgc taaggctttt
     2581 aaagataatt ggatagcgcc attaggtgaa aatgtggata aatttgaaga aagtattaca
     2641 aaatatactg gttctaaggg cggagcttct gcgttaagtt caggtacagc tgccatacat
     2701 cttgccttaa gactgcttaa tgttggaaaa aacgatattg tctttgctcc ttcatttact
     2761 tttatagcta cagtaaatcc tattttatat caaggggctg taccagtgtt cattgactca
     2821 gaacctaata catggaatat gagccctgtt gcactagaga gcgcattaga agaagctgaa
     2881 agaaacaacc aattacctaa ggcaatcatc gtagtgaatt tatatggtca aatggctgat
     2941 tatgaacgaa ttttagctat tgcaaaccaa tttaatgttc cagtgataga agatgcagcg
     3001 gaatcgttag gttctactta taagggaatt tcgtcaggct tatttgggac aattgggatc
     3061 tattcattta acggtaataa gatcatcaca acctcaggtg gaggagctct agtgtctagt
     3121 gataaaaaat atgttgagca ttcaagattt ttagcaacac aagcaagaga tcaagcaccg
     3181 tattatcaac atagcgaaat aggttataat taccgtctaa gcaatatttc tgccggtatc
     3241 ggacgtggac aaatggaagt attgaatgat agagtagcta aacgaagaga aattttcaat
     3301 cgatataagg aagctttttc tggaattgat agaattgaaa tgatgccaga agtggatggc
     3361 atggtgccaa atcgttggtt gtctacttta acgatcaatg attataattc tattgatcaa
     3421 gtagttaacg taataaataa actaaatgaa aaaaacattg aaacaagagc tttgtggaaa
     3481 ccactacatt tgcaaccagt atttcaaggg gtaaaaatgt attaccatga taataatctt
     3541 agtgtgtcag aaaagttatt taatagcggc ttatgcttac cttctggaag taatatgaca
     3601 gatagtgaac aagaatttgt tattgatgag tttaaaaaaa tattcaagta ggtgtgatat
     3661 tttatgaaca taggaattat tggtttaggt tccatgggga aaagaagact tagactcatt
     3721 caagaacact ttaatgatat tttactatgt ggtgttgata ctaatgaggc gagacgaaag
     3781 gaagttgaaa aggaatttgg aataccaact tataatagtc taacagaggc aaaaaatttg
     3841 tttgaaataa attcagtttt tgtttgtaca tcccctataa cccatgaatt tattataaaa
     3901 gaggctttaa atttagattg taatatcttt acggaaatca atttattaaa tgaatattat
     3961 gatgaaataa tcgaagtagc taaaaagaaa gatagattac tttatctatc ttctacattt
     4021 ttgaagagaa aagaaattca atatattaag tctaaagtga cgcataataa aaaaatttct
     4081 tatcaatacc atgtaggaca gtatttaccc gactggcatc cttgggaaga ttatcgcaat
     4141 ttttttgttt caaacaaaaa aactaatggg tgtagagaaa tattcgctat tgaattacct
     4201 tggattatag atacatttgg gaaagttaaa tcgtttactg taatgaccca gaaattatcc
     4261 aatcttgata ttgattattc ggacagtttc actcttatta ttgagcatga aactggtata
     4321 gttggaactt taagtgtaga tgttgtttca agagtagcta aaagaaattt aactataatt
     4381 ggtgaaaaga ttttaataga atgggaaggt aacccggatt ctctattttc atggaatgaa
     4441 gaaacttcta gtatgacaga tattcaattg tacgatgagg ttatgcatga taataattat
     4501 tcacatacta ttattgaaga tgcatattta gaggagatta aagaattttt cacgcttttg
     4561 gaaaagaaaa tggaacccca atatactttt gaaaaagata agtatattat taacttaatt
     4621 aacgacattg aggtaaatta atgatgaaag taattttttt tgggttaggg tcaattggaa
     4681 agagacatct aaaaaatcta ataagctatg gaaaaaagaa ttctattaat tttgatataa
     4741 cagcttatag tagcagtgga aaaaaagagt ttgaagaaat aacgtatatt caaaatagag
//
LOCUS       DARBFO010000134         4794 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000134 DARBFO010000000
VERSION     DARBFO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.139"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..290)
                     /locus_tag="SCK51_002730"
     CDS             complement(<1..290)
                     /locus_tag="SCK51_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEG1253293.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
     gene            complement(283..624)
                     /locus_tag="SCK51_002731"
     CDS             complement(283..624)
                     /locus_tag="SCK51_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253294.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(798..1523)
                     /locus_tag="SCK51_002732"
     CDS             complement(798..1523)
                     /locus_tag="SCK51_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEG1253295.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            1576..2052
                     /locus_tag="SCK51_002733"
     CDS             1576..2052
                     /locus_tag="SCK51_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253296.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            complement(2058..2240)
                     /locus_tag="SCK51_002734"
     CDS             complement(2058..2240)
                     /locus_tag="SCK51_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253297.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(2240..2539)
                     /locus_tag="SCK51_002735"
     CDS             complement(2240..2539)
                     /locus_tag="SCK51_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253298.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(2644..2847)
                     /locus_tag="SCK51_002736"
     CDS             complement(2644..2847)
                     /locus_tag="SCK51_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1253299.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(2862..3608)
                     /locus_tag="SCK51_002737"
     CDS             complement(2862..3608)
                     /locus_tag="SCK51_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEG1253300.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            3686..3994
                     /locus_tag="SCK51_002738"
     CDS             3686..3994
                     /locus_tag="SCK51_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253301.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(3965..4222)
                     /locus_tag="SCK51_002739"
     CDS             complement(3965..4222)
                     /locus_tag="SCK51_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HEG1253302.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(4520..4660)
                     /locus_tag="SCK51_002740"
     CDS             complement(4520..4660)
                     /locus_tag="SCK51_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253303.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
BASE COUNT         1321 a          933 c          749 g         1791 t
ORIGIN      
        1 tctgtttccg tatgtccttt aaccgttact gagacagata cataagtccc tgtttcatcc
       61 ataagaaaag gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg
      121 tagtgtttat actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc
      181 cacgcgtgag cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac
      241 ttacgactaa aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc
      301 ctccatttca gcaatgtatt tcttacctga tccgtaataa gagatatcaa tcaagttatc
      361 tctgttgtac tcttctagcg catcaatcaa gccatcttcg atgacataga tgtattcagg
      421 tttatttgaa tgttttgata gatggataag gtaaacatga tcccaaatac tcacaaaatt
      481 tcccaagtca tcttgatcac atgatagttc ttcgttcgtc aaaagatttc gtctgatttt
      541 tcgattattt gtttccttga tattcgactt gccccattca gaatcagtca aatattgatc
      601 taaagtggaa agttctttat tcatatgcta aaattctcct tagatatgtt ttgtgactct
      661 atgcttgccg gctgagtcac ttttttattt gttgccatgc tttttgcttt tcgatatgtt
      721 gttggcttag gatgtttggt ttattgtgtc tccaccagcg attagcaatt accgtcccta
      781 ttcttagcgc ttcagctcta ttcattttca tcaccgaaaa gtctttgttg tctgttcagt
      841 tgatcgattt ccatgcggat cgcagtttct ggtaaccaca tttcaataaa tgaaacagca
      901 tcatcgaatc tcttacgagg taactcgcca tatcttggga ttgaaaaggt acgtttaaat
      961 tcagaccaaa attttgagaa tacttttttg ctgatttctt cataagctcg gctttctttt
     1021 ccccctagaa cttccataac tttcatattt cctttttgct taatttcaaa ctcttgttgt
     1081 ccgctaattc gcatagtatc tttaagcatg gaaacatctt ttttaacatc tttcatttct
     1141 tctaattgat agatcatcat gtcttcaatt gtttgaggaa cagtattctt ccgaataaca
     1201 tcttccattt cgttgaaagc ttcaataaat tttagtttga aatttattgc tttacttcca
     1261 gtgaatccca tagctagcaa ggaaaatccg tctctattca tgaaataaac tctccgactt
     1321 cttccgtatg agtctggctc gttcccttcc acaaacatct gtccaaaatt ggacccatct
     1381 tcaacattgg ccgaattttc gaccgatctt tttattgatt caattgcttc tagtacatgc
     1441 ttatgtttct ttttgaaact ttctgccact tgtaagctcg tagttacagc ttctttattt
     1501 ttcaaaatta ctaattcttg cattatttct tctctccttt ttggtataat tttcgttaga
     1561 aagtgaggtg attctatgag gaaaaagctc aattacattc ttactgcgac tttaaccata
     1621 tatataattc tgtttattac cttatctttc ctgtcttcaa ttaaaaaaat tccttttatt
     1681 acattgaata aaaatagtat tttgaaggag ttcaataaca ctgactacat cgcatttcta
     1741 aaactttttt cagtctgtgt gctaatatca tgtgtaatta tttttattgt tgctaaaaca
     1801 tgttggacaa tgttaaataa aaaccaacgc gatgttgaaa atattgctat atttttaatt
     1861 aagattactt cctcaatatc aattatcatt ccgatgactg taactttttc aaaagaacag
     1921 tttaatgtag cggctactta tctttcattt atagcgctat tttcttttgt tcttcctaaa
     1981 agaaataaga gcaactttga caacaaggag ctggatgaag tacgcaatag cagcccaaag
     2041 gaacaaaaat aaaaaaatca tattttagtc agtccctccc gactggcttt tttcgctctg
     2101 tgctcagctt catcaagccc cataaaaatc caaaccatgt aaacgattgt ccctatcaac
     2161 gcttgtctgc ttccccagag gcctaaagcg tagatgatta gcggtgcgct gaatactagt
     2221 gctctgttga atttgcccat taacttgcct ccttcaaaac atctgaaggc gataaacttt
     2281 ttacacgata tttgtttgcg tctttccact ttaaaaacca aataaaagta tggtaatgaa
     2341 taaatgtggt tgagtgacct ggacgaagta ttccttctgc aaattcagga attgtttcca
     2401 tttccttgca gtattcaact aaagtagttt ttgacatgcc gtggaacttc tccaagatta
     2461 aactttgtcg gtaccagtcg tctggtttta ctgccttctc agcagcttct atcagctctg
     2521 aaagtgttgg ttttttcact tattatccct cctaaaaatt atcgttccat actcgatgtt
     2581 taacatttct tgaagctctg attttgtaag aatgatttta tcttccaacg actttacctc
     2641 cctctacaat tcgtacatag tgataatcga atctataatt ctgtttgctt ctgcagaagt
     2701 ctttttaccg tttaaaatta aagataagta gcttttgcta attccaaatc tttcagcaag
     2761 catggtgtaa gttaagaact ttgaactttc gacatattct ttgatttttt ctctatctcg
     2821 ttgagtgatt tctgcaatgt cagtcatact aaaactcctt tctaaccaat ttcctctaaa
     2881 tccatttgag ggtaatagcc ctctgcgatt aataagttgt aaataaacac tcgccctttc
     2941 tgtgtccatt tggtattcat tacaacttta gtgccgccat cggctttcgg tatctcactt
     3001 gtgtgcgatt ttgtgtatcc ttggttcata tgttttcggt aaagtatcca ttgtccgcta
     3061 actttatgct gaacacctaa ctcgtttagc aatttgttta gagcaattgc cgacattccg
     3121 taatcagctg caatttgaga agtagctacc gtatctgtcg atgaaagaat cgtatctaag
     3181 tagctaatct ttggttcgta ctcggcaatt tgttgttcga gtaattggtt cttttcttct
     3241 aaatccgctg ctaaacgcaa tgcttgtgca aaactttgag gtacgtttga atagctacct
     3301 gtttttctaa ttgttggaag gacttcactc gttacccaac gtttaaattt tttggcagag
     3361 ggaagttttg attttaagat taaactgtaa aggcctgact cgttaatcaa aacaactgct
     3421 gttccattga cggtgaacga atcgttctgc gtcttatcct ccaagtccac atgatctcga
     3481 atagcttttt gcggattcga atatcctaga acgtctgcta catcttttcc tacaaaatat
     3541 ggttcatcat ttactaaaat agttcgaact tcgttttgct cgaaattaaa aatttgtggt
     3601 gtattcatat ttctcattcc tttctttggt ataatttcct tatcagtgtg acaggctgaa
     3661 ataattgtta aggaggtgaa aatttatgaa accagaattt gtaattacta tgcttgatgg
     3721 agaaaaaatc aaaatatttg ccgatacttt ggtcgttgga tacgatgatg cgtcaaatat
     3781 cgaagaaaaa gatggaatgt tctattcttc tcaaatttac gtgaatacct tgcaaggtga
     3841 tattggggta tctactcttg caacatcaag accgcttgta ggtcttatcg gctttttgtc
     3901 aaaagcaaca ttcttttctc tcggcgatga tatagattcg gatgaaactg tcatctataa
     3961 aacatcagct gttaaaagta tcggtttcaa atagtaattc ttgctcgttg atagcggaag
     4021 ccaacttttc ttccaacaca tcggcgagct ttctttttgt tggattcatt tcaaagttga
     4081 tagtcttgag aagcattttt gcatccttaa ctttttgtcc ttgcaattcc ttgatgattt
     4141 tatcggcttg gctatcaatt ccatgtgtta gatcatctat cattttttgt accttgattg
     4201 tttggctcat ttccttgttc attgtttagc ctcctttaat aaatgatttt ttgtattttt
     4261 taatttccat aaattcctca aattgctctt tggtattatt accacgacca tacatattgt
     4321 gaaattcttt atggcaactg acacaaagtg taacgccgtt tgaaatcgct gcggatttag
     4381 ttgttgcatg gctaaatcac gaatcacaag tatctactaa aggcgaatca atttcgccaa
     4441 aagaaattgt tcaagcttat ttagatattc attatgctgt tatctatggt cagcttccag
     4501 aagaccgtaa aaacgatgac taaacgtgag ctaaaatctc tgctatggct gcaaccatgg
     4561 cagagtcttc tttatctacc gcgtgttcca tcgccacttt tgcttgtttc agtatgtcta
     4621 atttcaaatc ttcgattttt gccgagactg ttttttccat attcttctct cctttctttt
     4681 atattcgtaa acaaatttaa caacttttta aaaaactgcg ttgactttat tagattaata
     4741 atctatacta aatgcatagt taaataagac atataaactt tgatttaaaa agct
//
LOCUS       DARBFO010000135         4788 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000135 DARBFO010000000
VERSION     DARBFO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.140"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            564..989
                     /locus_tag="SCK51_002741"
     CDS             564..989
                     /locus_tag="SCK51_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253304.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="SCK51_002742"
     CDS             1019..1183
                     /locus_tag="SCK51_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253305.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="SCK51_002743"
     CDS             2141..3118
                     /locus_tag="SCK51_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1253306.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="SCK51_002744"
     CDS             3128..3625
                     /locus_tag="SCK51_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1253307.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="SCK51_002745"
     CDS             4001..4282
                     /locus_tag="SCK51_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253308.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="SCK51_002746"
     CDS             4284..4700
                     /locus_tag="SCK51_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253309.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1758 a          817 c          785 g         1428 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtacacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DARBFO010000136         4775 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000136 DARBFO010000000
VERSION     DARBFO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.141"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(20..613)
                     /locus_tag="SCK51_002747"
     CDS             complement(20..613)
                     /locus_tag="SCK51_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1253310.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(645..1724)
                     /locus_tag="SCK51_002748"
     CDS             complement(645..1724)
                     /locus_tag="SCK51_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG1253311.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            1841..2765
                     /locus_tag="SCK51_002749"
                     /pseudo
     CDS             1841..2765
                     /locus_tag="SCK51_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2779..3480
                     /locus_tag="SCK51_002750"
                     /pseudo
     CDS             2779..3480
                     /locus_tag="SCK51_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(3677..4756)
                     /locus_tag="SCK51_002751"
     CDS             complement(3677..4756)
                     /locus_tag="SCK51_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1253312.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT         1350 a          936 c         1065 g         1424 t
ORIGIN      
        1 tttttgtgtt agataagact tattgcaaat gctgataaaa atagatagcc aattgcgtgc
       61 gatcccgtaa atctagcttt tctagtaatt ggctgatgta attgcgaaca gtgccgtcac
      121 tcaagtacaa ggcatctgca atttctttat tgtttttccc ggcagctact tctttgatga
      181 ttgcaagctc acgttcacta aaagaagcat aagcatgact atcaatcgtc ggtttattct
      241 tcatcatttg agtgaaagtc tcgatgattt cattaccaaa tacggcctgt ccattataag
      301 cggatttgac agaaggaata atcgcttgca agttctgttt gattaaatac cctttgacac
      361 cgatggaaaa tgcttcgcgg atgtattctt catctttgaa tgtcgtcaaa agcaaaagga
      421 tggctttggg aaatttagcc aaaatttctt tggcagcatc gatgcccgtt tgtttaggca
      481 tgcggatgtc cgtcagcaaa acatcgggac gatgggtttc gtataagact agcgcatcct
      541 tggcacaatg accaatgcct aacacacgga tctctttcgt gctttctaaa atggtttgta
      601 acgatttcgt cacaaatgga tcatctcaat aatgactact gttatcattg gtgtccctcc
      661 cttggtaaaa tgatcgttaa ctgaaattgc tggttttctt ggaaaatatg gacttgtccc
      721 ttttgttctt gaacacgttg acgcatcgtt ttcaaaccaa taccgggagc agaatttttt
      781 tgtgggctgg tgccatcatt cgtgatttgc aagcgataaa aggccggcaa cgttcgaaaa
      841 tgtaaaacaa cggttttggc atgactatgt ttgatactgt tggttaaggc ttctttgatg
      901 accattaaga agcagttttg ctgcgtttca ttcaatcctt caaacaagtt tccttcaatc
      961 gtaacagaag aaggaaaatc agcgatcaga aactctaagg agtgttgaaa agagatcgat
     1021 gtctcatgga ggtcatggac actttgacga atccgatcca tcccagtgtg aacggtggca
     1081 cttaaatttt ctaatggttc ttttagtttt ggttcttgat tgatcatttc aagcgcacca
     1141 atttgtaaaa tcgcacttga cagcagatga ccgacattgt catggatgtc tcgggcaatt
     1201 cggttccgtt cttccacaat ttgcaaggct aattgtgttt cttgtgacag ggccaatgct
     1261 ttattttgtt ccgataactc ttgttgtttt tcccaatgag tatctttgga gacaatgtac
     1321 tttttctcaa gatcggtcgt tcgttgatct ttttttcgta agtaaaagga aaaaaacagc
     1381 aaaattacga caaaggcgac cttgatgggg ggatgattac caaccgtcga aatcaacaaa
     1441 aaacctaaca aaaacaagta atcaaggtgg ctttcttcag gggcgatcac tcgcatcgtc
     1501 gctggcagaa acaggacaag atcaggtaaa aaggcacata aaagaaatag aataacttgg
     1561 aaaatccaat ttggctttgt aggcagtaaa taactgacac cagtgaataa aacggcgatc
     1621 aataaataga taacttgcat agcaacgaat ccttgactta ccagcaaaag caatgagata
     1681 accagtagaa agctagtttc gttgagtagc cgagatttat gcatagcaga ctccttcatt
     1741 gtgacaaatg tcatgtgtgg tttagccaat cgtcactagt ttcttttctt gcactttatt
     1801 atagtcagat caacaagaaa tttggaagga gtttttcatg atgattatcg aaattgataa
     1861 attagtcaaa cgctatggag attttgtagc attgaatcat tttgatctgc aagtacaaga
     1921 aggagaagtc ttaggtctgt taggaccaaa cggcagtggg aaatcgactg ccatcaactg
     1981 tatgctttct ttgttaaatt atgacaaagg aaccattaaa ttgttcggga caacaatgac
     2041 tcctgaaaat tatgctgtca aggcaatgat tggggtcgtt cctcaagacg ttgcggtctt
     2101 tgacgaacta agtgtgcaag aaaatatcga ttatttttgc ggtttatatg tttccgaaaa
     2161 agagaaaagg aaagccttga ttgaagaggt gattcaatta gtgggattag aaaaatatgt
     2221 ttcgtttcgt ccaggagaac ttagtggtgg cttgaaacgt cggttgaaca tcgcatgtgg
     2281 gattgcccac aagccgcggt taattttttt agatgagccg acagtagctg tagaccccca
     2341 aagtcggaat aaaattttag aaagtatcaa ggagctgaat cgacaaggag caaccattgt
     2401 ctatacaaca cattacatgg aagaagtaga aattctttgc gaccagatcg tgatcatgga
     2461 tcaaggacag gtgattgcta aaggaaccaa agaatctcta aaagatatgg tacggacggc
     2521 ggatcgtttg acgatcgaca tcccattact cccagaaaac gtgttgtcag cgaacaatag
     2581 cttctgatgg aatcgcttat aaaaaaggtc agctccaagt aacaactact gaactaaata
     2641 cagtattgtt gccgattctt acaatcttac aagaagaaaa gatcagttac ggaaaaatag
     2701 caaccaaaga agcgtccttg aatgatgtct ttttagagat tactggcaag gatttgagag
     2761 actagaggag gggaaataat gttttggcat ttttataaat atcgcacaaa aatactgctg
     2821 cgaaataaat ccatgttgtt ttggacattg gcctttccaa ttcttttagg attgatgttt
     2881 atggcggcct ttggcgagat tgatcaaggt ggcaatctcg aaacgattaa tactggaatt
     2941 gtaatcaaag cggatcaaac agcgctttcg gaaaatttta ccacagtctt agaacaagtc
     3001 gaagtcaatg ataaaccatt atttgaagta caggaactat cggaagtaga agcgaatgaa
     3061 aagctaaatg cagatgattt ggctggtttt tatctcattg atggttcatc tgtttcgctc
     3121 catgtgggtc aagcaggaat gagtcagacg ttgatgaaaa acgtcatgga tcaattttta
     3181 cagcgtattg gtataatttc gagtaaagtg gcagaagtgg agaacgttga tctcgcgccc
     3241 atcatgactg tttttgaaca agaagtatct tttcaagaag ttcatgcagg tcgtaatatg
     3301 agtttcaaaa gtttttattt cttcactctg attggaatgg cgattctcta cgggacgatg
     3361 tggggggtcc gcaatgtaca ggatcagcaa gcaaatcaat cgtcaaatgg tattcgcttg
     3421 tccttgattc ctagaaagca atcgttagtt tcaatcgcta atttggcagc aagctttacg
     3481 tccgtaaatt tcaagtttaa gttagccact acaggtaact catttgtttg ataccgtaac
     3541 tgcctcgtta ttctaaatca gtgaggcctt gataatgaac tgaggtatga tagtcttcag
     3601 acgattctgg ccccaggcca gctttccaga catgaagcca tgtcccgagt gaggatcgtt
     3661 atcaaggaat aatgggtcaa gccaccaacc gttcttgctt gaatgcctgg acgaatgtat
     3721 catgtggtgt cgcataaccc aaaattttcc gcgggtaatc gttcatccaa cgctggatcc
     3781 gcaaaatctg gttctcgctc acctcactaa tcgggcttcc cttggggacg aaacggcgaa
     3841 ttaatttgtg ctggttttcg ctcgtcccac gctcccaaga agcatagggg tggctgaaat
     3901 agacgtcagc tacatcttgc agagcatcgt gtagcccaga aaactccgag ccattgtcgg
     3961 atgtaacggt cttaaataat tgatcgaagt tagcgccagc ccgttctctt agctgttgaa
     4021 ccgcttggtc caccgattcg gtgtccttcc cgttcaactt taggatgact tcaaagcgtg
     4081 tctgccgttc agccagcgtc agcagcacgg catccgactt ggtcttgttt cccactaccg
     4141 tgtcgatttc ccaatgaccg aacgtttccc gtgtttcaac ttctttaggg cgttcttcaa
     4201 ttgacgtccc gagaacacgc ttgttgggac ggtgtcctgg gcggaccgct ttgattttac
     4261 gggacagttt ttcaagcaag tccatgttct ttgttcgcat gattccgcgg tcaatccatt
     4321 gataaagagc ggttgtgcca ggaatgatgg cacaatcgaa cagctcgtgt tttctggcaa
     4381 atccgaccac gacgtccggc gaccattttt catttaacat tttgtcatct gcccaatcaa
     4441 tgaaggcatt tgtgttcgcc catttggggc gtctgccaca gttcagtcgt tgcttttcat
     4501 agaaggcctg tcctgcatca gcgtcataaa cagaatagta ggtgtcatac acttttccat
     4561 tttgcgtttg gcgttttaac tgggtgatgg ttccgcgatc aatctcattg tttatggttt
     4621 gcggaacacg tcccagcact ttggcaacct gacggttgga atagccttcc tttttcagga
     4681 tagcgatttg gcaacgttct gagtaggaaa gatgttttcc cttgcgtgaa actgtgttat
     4741 catgggtttg cgtcatgtga attcatcctt gtcta
//
LOCUS       DARBFO010000137         4712 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000137 DARBFO010000000
VERSION     DARBFO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.142"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            106..357
                     /locus_tag="SCK51_002752"
                     /pseudo
     CDS             106..357
                     /locus_tag="SCK51_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="SCK51_002753"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="SCK51_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEG1253313.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="SCK51_002754"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="SCK51_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="SCK51_002755"
     CDS             1786..1869
                     /locus_tag="SCK51_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HEG1253314.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="SCK51_002756"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="SCK51_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEG1253315.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="SCK51_002757"
     CDS             complement(2994..3857)
                     /locus_tag="SCK51_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HEG1253316.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="SCK51_002758"
     CDS             complement(3859..4131)
                     /locus_tag="SCK51_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG1253317.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4357)
                     /locus_tag="SCK51_002759"
     CDS             complement(4148..4357)
                     /locus_tag="SCK51_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEG1253318.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4712)
                     /locus_tag="SCK51_002760"
     CDS             complement(4455..>4712)
                     /locus_tag="SCK51_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HEG1253319.1"
                     /translation="MIGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKV
                     STWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1446 a          796 c          865 g         1605 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc at
//
LOCUS       DARBFO010000138         4647 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000138 DARBFO010000000
VERSION     DARBFO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.143"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(133..717)
                     /locus_tag="SCK51_002761"
     CDS             complement(133..717)
                     /locus_tag="SCK51_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1253320.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(928..1131)
                     /locus_tag="SCK51_002762"
     CDS             complement(928..1131)
                     /locus_tag="SCK51_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253321.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1141..1563)
                     /locus_tag="SCK51_002763"
     CDS             complement(1141..1563)
                     /locus_tag="SCK51_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253322.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1801..2415
                     /locus_tag="SCK51_002764"
     CDS             1801..2415
                     /locus_tag="SCK51_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1253323.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2580..3404)
                     /locus_tag="SCK51_002765"
     CDS             complement(2580..3404)
                     /locus_tag="SCK51_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253324.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(3428..3583)
                     /locus_tag="SCK51_002766"
     CDS             complement(3428..3583)
                     /locus_tag="SCK51_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253325.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
     gene            complement(4042..4299)
                     /locus_tag="SCK51_002767"
     CDS             complement(4042..4299)
                     /locus_tag="SCK51_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG1253326.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4292..4561)
                     /locus_tag="SCK51_002768"
     CDS             complement(4292..4561)
                     /locus_tag="SCK51_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1253327.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         1534 a          816 c          626 g         1671 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      781 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa taaagcaaaa
      841 aaagaaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa aaaagaatgt
      901 cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt tttggtctct
      961 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
     1021 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
     1081 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
     1141 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
     1201 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
     1261 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
     1321 tatcgtttta gaagctgcta gagcaagtaa tggagaaact attaaataat tttcagtaac
     1381 agttaagtcg ttagttgggt taccaatggc tattccgtcc ctatctaaag gagttaattc
     1441 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
     1501 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
     1561 cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga
     1621 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
     1681 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
     1741 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
     1801 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
     1861 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
     1921 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
     1981 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
     2041 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
     2101 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
     2161 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
     2221 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
     2281 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
     2341 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
     2401 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     2461 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     2521 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     2581 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     2641 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     2701 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     2761 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     2821 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     2881 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     2941 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     3001 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     3061 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     3121 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     3181 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     3241 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     3301 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     3361 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     3421 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     3481 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     3541 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
     3601 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
     3661 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
     3721 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
     3781 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
     3841 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
     3901 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
     3961 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
     4021 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
     4081 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
     4141 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
     4201 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
     4261 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
     4321 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
     4381 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
     4441 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
     4501 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
     4561 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
     4621 aaatgattaa tattctaact tcttata
//
LOCUS       DARBFO010000139         4635 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000139 DARBFO010000000
VERSION     DARBFO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.144"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            175..519
                     /locus_tag="SCK51_002769"
     CDS             175..519
                     /locus_tag="SCK51_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEG1253328.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="SCK51_002770"
     CDS             520..939
                     /locus_tag="SCK51_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEG1253329.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1031..1381)
                     /locus_tag="SCK51_002771"
     CDS             complement(1031..1381)
                     /locus_tag="SCK51_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253330.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1347..2567)
                     /locus_tag="SCK51_002772"
     CDS             complement(1347..2567)
                     /locus_tag="SCK51_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEG1253331.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2933..3499
                     /locus_tag="SCK51_002773"
     CDS             2933..3499
                     /locus_tag="SCK51_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1253332.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3644..3731
                     /locus_tag="SCK51_002774"
     tRNA            3644..3731
                     /locus_tag="SCK51_002774"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Ser,seq:cga)
     gene            4065..>4635
                     /locus_tag="SCK51_002775"
     CDS             4065..>4635
                     /locus_tag="SCK51_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEG1253333.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1685 a          778 c          891 g         1281 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gatagtagat ttccatacta gggtcccgag
     1201 gattgttcag caaatcagca agtacatcta aatcttctgg ctgagtttct gccgtaccct
     1261 gtatctctat gaaaagccct tcctcagcaa attctactcc aatacgtatt tcagttattt
     1321 catgggaata taaataagta agaagctcat cgatgattct ttttgataat tctttttcca
     1381 agtgtctttt tttcctttct gacagcggta aagttatcta gtatcggtac aaggatacct
     1441 gcaacgaaac cagttgaaaa accactgtta tatagattta gtcctccgtg caaagtgctg
     1501 acgttatgga caagagcaat atgtagtaca ccagctatta caccgtaaaa actgccgtaa
     1561 aaaccgctta tgggcgcgag acctgtccca aacacagctg caacaagcat gccagtactg
     1621 gtgatctcat gaaaagttcc aaacaaagaa gcaataaata ttccgactag tatcggaaaa
     1681 ctgtttttta aatgacaacc aaaagcactg aaacccaccg cagataaaat agcaccgatg
     1741 acaggtccat ttaactgacc gccaaccagc aaaacataag aagacagcat cagaccaact
     1801 aagcctatgt tgatcaaagt agcaccaata ccagaattag caatgaaatc agttgtcaat
     1861 tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc gaaaggaatt ataatagaaa
     1921 ccaattccga ctgtcagcga aaaaaagcca aaaataatcc aaatgagagg aaagtgatat
     1981 tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga ataacctcaa aacagcagta
     2041 aaagtcatcg caatcaaccc agaggtgaat ccaatattat aaagtgtgaa tccacgatgg
     2101 aaaactaaag tttgagcagc taagggcgga agaagtagac caattgctat gccagctaaa
     2161 ttccccaata aaatacctat cagtaaagga aaacgcatac caaaagtgat ataactaacg
     2221 accggtgata atgcgctacc aaacagtccg atcatgatgt actgagaaaa aggtttattg
     2281 acaaatacag cgtatagata cacacctaaa atgattgaga tagaattata aaggtttttg
     2341 ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa ataagggacc gtttaaaatc
     2401 accttgtgcc gataagctaa aagcacagaa agtaaggtca aaatccctac atttatgaat
     2461 gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga
     2521 atagttagca atccactcca attatcagta ggagtattag aaagcaagcc aataaaaaga
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa
     2881 atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt taatgagaag
     2941 aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg caaaggaagg
     3001 gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga cacagccgat
     3061 ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt ctatctttga
     3121 acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca ttgatctcgg
     3181 tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat tgtacattga
     3241 tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc gtgaaatagt
     3301 taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc tgcatactgg
     3361 tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca tgcatccaac
     3421 agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag cattagataa
     3481 tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt ccgttggctg
     3541 caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat ctattagaaa
     3601 taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt tggcagagtg
     3661 gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc
     3721 tgtaccttcc ttatatcaac gattagaggt tgtgaagaag ctgtcctgca tcaagtaata
     3781 agaacggcca aacaaaaata gcttttccta ttttttcgtt tgattgggct tattaccgca
     3841 gatgcaagct tttgaacacc gtttgttcgg aataaggggc tgtgacaaga cttttgtcac
     3901 agccccttat tgttgtactt ttaacctaca atattttttg attccccctg agtactattt
     3961 tttataaatg ctagtaaatc aatcttcttt ggcctttttt tgataaaaaa aatactagat
     4021 gtaagataaa agtgtaaaaa cgatttgttt taggagggga aacgatgtgt accgcaatca
     4081 cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg tcttataatg
     4141 aagtggtcac tattactcca agaaattatc gtttcgattt tagaaaggta aaaaatttag
     4201 ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca ctctattatg
     4261 aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc aatgctgatt
     4321 ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt ccatggatac
     4381 tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc aatctagtca
     4441 atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta gctgataaag
     4501 aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat aatcctgtcg
     4561 gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac aattatcgct
     4621 cgctatcaaa tggaa
//
LOCUS       DARBFO010000140         4611 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000140 DARBFO010000000
VERSION     DARBFO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.145"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(86..340)
                     /locus_tag="SCK51_002776"
     CDS             complement(86..340)
                     /locus_tag="SCK51_002776"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1253334.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(451..705)
                     /locus_tag="SCK51_002777"
     CDS             complement(451..705)
                     /locus_tag="SCK51_002777"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1253335.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(898..1173)
                     /locus_tag="SCK51_002778"
     CDS             complement(898..1173)
                     /locus_tag="SCK51_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HEG1253336.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1145..2098)
                     /locus_tag="SCK51_002779"
     CDS             complement(1145..2098)
                     /locus_tag="SCK51_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG1253337.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2710..4203
                     /locus_tag="SCK51_002780"
     CDS             2710..4203
                     /locus_tag="SCK51_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEG1253338.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4338..>4611
                     /locus_tag="SCK51_002781"
     CDS             4338..>4611
                     /locus_tag="SCK51_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEG1253339.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1543 t
ORIGIN      
        1 ttgcttgaat tcttgtgaaa aagttcttcg tgttctctta gacataaaaa ttcctcctgg
       61 tatgttttct tctagtctac acaccttaat ttttctgtct agttaattgt agcctatcca
      121 tgatcggttt attagacctt tgaggtctat attttttaac tacaatcgac cttaaattta
      181 attgtttcat tagcttttgg acatgtttaa gagatactga aatcccttct tttaataaaa
      241 cttgatggat ctttgtggca ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct
      301 tctttttcaa cttattattt ctgatttctg tagctgtcaa cggtttattc ttataaaaat
      361 aatagacact tctagggatt tctaataacc gacacaacaa agaaacagtg taatccttac
      421 accatttttc aataaattga acaagcgaat ttaggttttc cgagagaata tggctgcggc
      481 tttttttaag atttcaagtt cctcatttaa acgagcattt tcttttctca gttcagctac
      541 ttcttttcca gtcagtcctg tggactgatc aggtaaatat aaattcttcc atttgtaaat
      601 cgttgcttca gataagccat attctttcgc taaccctcga acagaacgtc cagtttgatt
      661 cagttcaaca atcatttgtt taaaattttc ttcatatcgg gtcataaaaa ttcatcctct
      721 cgtagataag tttaatttat tcttgtctct acgaaaagct gtctaagatt tgatactaac
      781 accaaatgtt gtataccccc aaacaataca catattttga aggattagga ttaataaagc
      841 taaaaatata gtaagtatac taaatatatt tactatatct agtatactta ctatatttta
      901 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
      961 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
     1021 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
     1081 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
     1141 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
     1201 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
     1261 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
     1321 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
     1381 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
     1441 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
     1501 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
     1561 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
     1621 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
     1681 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
     1741 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
     1801 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
     1861 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1921 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1981 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     2041 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     2101 ttccctccaa tttcctatac taaatatatt tagtatactt catatactta atatattcga
     2161 tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat
     2221 actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg
     2281 tgcgttttgc gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca
     2341 gataattcac tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca
     2401 taaaaaaaag agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg
     2461 ttaggacatg tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt
     2521 aaaagttgat cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact
     2581 caacagaaga acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga
     2641 acacatacta tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg
     2701 ttacaatcga tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac
     2761 aaatatcgaa acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct
     2821 atctttgcgt accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg
     2881 gaagaagcta ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc
     2941 tttggtactt atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta
     3001 agtcaaatca acacatttgt agttgatatc gattcaaaag agaaccatca aggcgaaatt
     3061 attttagcct gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat
     3121 ggttatcaag tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc
     3181 aaggtgatcg atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa
     3241 ttgtccggag tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat
     3301 aatattgttt ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg
     3361 atgaagcttg aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt
     3421 cctgagaaaa aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa
     3481 gcaaacatcg tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct
     3541 ttagcttatt actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc
     3601 aacgaacggt tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct
     3661 tattctggta attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg
     3721 gtttcaccaa ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt
     3781 aaaaagccac gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg
     3841 gcttatctaa gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa
     3901 ttggcttcac aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga
     3961 gaggagaata aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct
     4021 tcagtcaaaa cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat
     4081 tcaacaagga tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga
     4141 caattatcaa cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt
     4201 tgaaaaataa aaggtgcaaa ttgccttata caatatatct atgatacttc tgtaggtgtt
     4261 gctccctttg cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt
     4321 atcatggagg aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt
     4381 ttatcattgg tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc
     4441 aggggacttt gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt
     4501 ttcttcatgg gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt
     4561 gtgtgttaga gcaggagtat attgagtata aaatagctaa cgagttaaga a
//
LOCUS       DARBFO010000141         4470 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000141 DARBFO010000000
VERSION     DARBFO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.146"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            318..1049
                     /locus_tag="SCK51_002782"
     CDS             318..1049
                     /locus_tag="SCK51_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG1253340.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1194..3221
                     /locus_tag="SCK51_002783"
     CDS             1194..3221
                     /locus_tag="SCK51_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEG1253341.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3329..4426
                     /locus_tag="SCK51_002784"
     CDS             3329..4426
                     /locus_tag="SCK51_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEG1253342.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1569 a          717 c          923 g         1261 t
ORIGIN      
        1 atataatata cattttttat ttaattataa ttaatattga ttgaagtcta ggcagtcaag
       61 tcacattttg atagatagga gggggactta cgaattattt taaaggctat gacagctgta
      121 tttctagatt gtgtttgcat caagttgcaa taaaaactag aaatcaaatc gggctgataa
      181 tcatttataa ctaatcgata cataggacca aatataggtc tatgttcttt tatttcataa
      241 gtatcgtaaa acagaatttt gctatggatt aatccaatcg actgatatgt ttagtaaata
      301 aagaaagagg aatgtcaatg atgaaaaaag aaatcgtatt aacagcagca atgatgttat
      361 tcagcatggt agcaagtacg acttttgttt ctgcaactga agtataccca aaggaatata
      421 acacagaagg aacgattaca tttgaagcag gagatgaggg tgtaactcct cctgttgatc
      481 cagagaatcc tgatcccaat aaacctgtcg atccatcaga tcctccaagc cctggaacag
      541 gaggggcatt atcgattgat tatggatcaa aattcaaatt tgggactcaa aaaatttcta
      601 ctgcggataa aacgtattat gcagctgctg atgtgatgaa cgatgggtct agaaaaccga
      661 catatgtcca agtgacagat aggagaagta ccttatctgg gtggaaatta agtgtctctc
      721 aaccagaaca gtttaaaact gccagtgggg atgaactagt tggagcacag ctgaaattta
      781 cgaaagggca ggctgtttct ttggttgatc caacatatac gccgcaaacg gtgaattctg
      841 aattaacgtt gacaccggga ggaaacaata cgttagcaat taatgctaaa tcaggaacag
      901 gtgtggggac ttgggtctat cgatttggtg caaatgaaaa tgaaaatcaa gatgcggtac
      961 agctttcggt acccggtaaa tctgtcaagt tagcgcaaca atattccact aaattagttt
     1021 ggacattaga agatacacct aacaactagt ttactgaaaa gaactgttaa aaataaatac
     1081 gtagtcatat tgtcttgcga ggtaatcgag caaaaattgt caaaggctag gctaaatttg
     1141 tttggatacg aatagttcat ctaggaattt aacgatcagg aggaaaagat atattgaaaa
     1201 agaggaggat cgtgctcatt ttaagcagtc tcttagtgac taatatcttt ccgatcatag
     1261 ggtactcttt ggaattgaat cagttggagc atggaaatag aagagacgaa gaaagcaaac
     1321 tcacttcgac attcgacagt ttaatagaat ccaaccagac acaagaaagc agtgaggatt
     1381 taagtatttc gagtagtgga gagaccactg acaacgtaga agctgagggt tcggacaaac
     1441 aaggactaga agatgcacca atttctgcgg ttgagctgcc tgacgatccc aatattattt
     1501 ctatcgataa aatttttcaa acacctatcg gtactagcac gtccatattg gaaaatggga
     1561 agttactgca gttgaaccca gcagaaaggt ctcaaagagg agctatttgg tcaaataaga
     1621 agattagttt actctcggat tttacattta aatcttattt atacttagga aatcaacgag
     1681 atagagcggg agatggcatg acctttacat tgactggcga tcctagaatg gctagtaatc
     1741 ccagtcaagt tattggatcg ccaggtatgg gaattggctc atattctact cgttctggcc
     1801 aaccttacat tgcgaatgcg ctctcgattg agtttgatac gtataaaaat agtggttctt
     1861 ctgatcgaat ggatcgagag ataactgctg ataatagagg gcatggacat gtcgcttttg
     1921 tcacgcctaa accaaataat aataattatt ctggagagca tactggcgtg acggtagctc
     1981 ctacatttct ttctaatggc acatggcgct tgttaataat taaatgggac gcggagcaaa
     2041 gaagtcttac ttatgatttg gaaggtgtcg gtcactcttc tcggattata cctgatctca
     2101 ttaggcagtt tggttctaca gaagtaaatt gggggtttac ttcttcaaca ggtacttttt
     2161 ttcaagaaaa tgctgtcgct gtcacgcgac ttccatcgaa tgtcacatca acagctgatg
     2221 taaaagtcaa cgatagtgat tatggggcca tcgctgaagc tggaggaaac gataagataa
     2281 ctatcagaaa tacattgaag gtcgaggggg attttcttga atataagaaa atgcagacta
     2341 gtatcgacct cccacaagaa ttaactattc ccgagcatat cttgatcaat ggaaatcaaa
     2401 ttgatgccaa agatatcttg ataaccggaa atcgagtcac gctaaatctg acagattatt
     2461 ttattgctgg agatacgtta ataatcgaat tggagacaac tttgcaggaa gtaattcctg
     2521 aaaaagtact cactatgcat tttgaatacc ttgaaaacgg gaatttgatt gagcaatcga
     2581 atgaaataaa cctaaagatc gcaaagctga aggagataga tatccatgtt tattataaag
     2641 ataaagacag tcagattgat attgctgaaa tgaaaacaat cactgggaaa ataggagaga
     2701 agtataaaga aactcctatt gatattgaag ggtatgtttt tgactcggat tcagcgaata
     2761 cagaaggcat cttctccgaa aatacagagg atatctattt ttattacaga aaaggcaaac
     2821 tttatctctc aactgctcct caatatgtca attttggaaa acataaaata tccagtagac
     2881 cattaagtga attcggacag ttttcaggtg ggttgaaaat tatagatgaa cgggcttttg
     2941 gaagttggaa attacaattg aaacaagaga aagttcttac aaatggagat tttgagcttc
     3001 cagaagctct ttcctttgtt acctctgaaa atacaacggt tatagggaca tccgctgtta
     3061 cgattttcga aagtgatcaa aagggtgaaa gcgatttgtc ctctctttta gattctacag
     3121 ctcatcgtgg aatcagaata gatattcctg tagaaaacca aagaatcgga acctttgagg
     3181 ggaaactatc ttggatactt gcagatgtcc caggaaatta acaggaagag aagaatataa
     3241 caaaattatt gagtttcaag aagggcacag gtaccatagg ctcttgtgtc ccacagagac
     3301 aagattttgt tatagtcttc tcttacatat gattaaagga gcgattttct tgagaccaaa
     3361 acaacaatta tttataacac tcctagttat tttaggggca ctttttaaca atatatcagt
     3421 gtcagctagt gcatttaatt tttcagtgac gccgataact tcagagaatc aaatcgacaa
     3481 gagaaaaact tacttcgatc ttcaacttga tccggatcag gaagtagaag taaaagcgga
     3541 attgagaaat gatacagaaa aagaagtgaa aatcgatatt tctgtgaata gcgcaaccac
     3601 caattcaaat gtgatggttg aatacggaaa aaacgagatt gaaaaagaca tgagcctgat
     3661 ctttgatctg gtagattatg tgagctatcc acagatagtg acgctaaaac ctaaaagtgt
     3721 ccaaaccgtt gcttttcatg tcaggatgcc gaatgagcga tttgatggtg tattggctgg
     3781 tgggatcaca ttccaagaaa tccagacgga aaaggaccaa acagaaacta aggatcaaag
     3841 cttatcgatt gagaatgaat acacctatat tattgctttg ttgatgcaac agacactgaa
     3901 cgaagttgct ccgaacctta ctcttcatga agttaaacca gatcaaatca atgcaagaaa
     3961 tgtcatctta gcaaacgttc aaaatgatca aaaaacctat atcaatcaag tagttattga
     4021 aacaaaaatt acaaaaaaag gacattctga agttttatat caagaggaaa aagaaggatt
     4081 gcagattgcg ccgaatacaa atttttcttt tccaactgct ttaaatggtc aaccgctaac
     4141 cccaggtgaa tatcacctta caatgactgt tttaggaaat gaaaatgaaa atggaaaatt
     4201 ttcgagaaaa aaaggaaata ctacgatcaa ctataccaat caatgggtat ttgaaaaaga
     4261 ttttacaata gacggaaaag tagccaagga attgaacacg acggatgtta cgattaaaaa
     4321 agacaataat tggatttata tcttagtagc ttcacttttc ttattgttta ttttattatg
     4381 catacttttg tttgttttga gaaaaaaaga agagaaagaa aagtaaggtt aagataacaa
     4441 atagtaaatt tttattattg taggatggtt
//
LOCUS       DARBFO010000142         4461 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000142 DARBFO010000000
VERSION     DARBFO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.147"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            574..1008
                     /locus_tag="SCK51_002785"
     CDS             574..1008
                     /locus_tag="SCK51_002785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253343.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="SCK51_002786"
     CDS             2298..3221
                     /locus_tag="SCK51_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1253344.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="SCK51_002787"
     CDS             3237..3776
                     /locus_tag="SCK51_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG1253345.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          667 c          758 g         1372 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DARBFO010000143         4425 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000143 DARBFO010000000
VERSION     DARBFO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.148"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            690..1658
                     /locus_tag="SCK51_002788"
     CDS             690..1658
                     /locus_tag="SCK51_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1253346.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="SCK51_002789"
     CDS             1669..2484
                     /locus_tag="SCK51_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HEG1253347.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="SCK51_002790"
     CDS             2552..3262
                     /locus_tag="SCK51_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1253348.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="SCK51_002791"
     CDS             3298..>4425
                     /locus_tag="SCK51_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEG1253349.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DARBFO010000144         4404 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000144 DARBFO010000000
VERSION     DARBFO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.149"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..580
                     /locus_tag="SCK51_002792"
     CDS             <1..580
                     /locus_tag="SCK51_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEG1253350.1"
                     /translation="HKEFYYKHIDVYQKHDYPRINRNNTTAFKIFRAIINRCLRMIKL
                     PPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLF
                     FNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKES
                     EKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            573..2000
                     /locus_tag="SCK51_002793"
     CDS             573..2000
                     /locus_tag="SCK51_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEG1253351.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            2002..3072
                     /locus_tag="SCK51_002794"
     CDS             2002..3072
                     /locus_tag="SCK51_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253352.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            3059..>4404
                     /locus_tag="SCK51_002795"
     CDS             3059..>4404
                     /locus_tag="SCK51_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEG1253353.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLT"
BASE COUNT         1516 a          536 c          685 g         1667 t
ORIGIN      
        1 acataaagaa ttttattata aacatataga tgtatatcaa aaacatgatt atcctagaat
       61 taataggaat aacacaactg catttaaaat ttttagagct ataattaatc gttgtctgag
      121 gatgattaaa cttcccccaa aaattagaca ccataaattt gatttgtatc atggttctca
      181 atggtgggct ttgtcgaaag aatgcctaac agaattaata caaatgtatg aacaaaatca
      241 acaagactac ctgaatttta aaataggtat gtttgcccca gatgaaaaat tttttcatac
      301 actttttttt aattcctcgt tcaaaaataa aaatgttatt ggaggaccag atttaccact
      361 tgaattgaaa aatattgaag aaactacttt acaaacttcg aaattagcaa atattcatat
      421 tattgatcct tccatgaaca aatggtttga tgaaagggat atagatcaaa taaaagaaag
      481 tgaaaaatta tttgtaagaa aagttaaatc ggattactct gataaactat taaatagaat
      541 tgataaagaa attttgaaag ttggaaatat agatgaatag aatagtacct gttttagaga
      601 agagtttatt aagtcgagat gcgtttcgag taataaattt aatcatttgt ctgctttatt
      661 ttatgcctag tacttttaat gtaactaata tcccgatgaa aatagctttt gcttggggtg
      721 gaattatagt tttgtatgag gtttttataa agaaaaattt ttttaaaatg cgtttttctt
      781 ggctactgat tggagcaatg attagtttta tcttaactat aatcattaac agagaaaatt
      841 ttttaattcc atctatttat aattttggat atttgctgat tactttgata gtaatttttc
      901 ctgcagactt ttcacaaagt gaagaggaaa aaaagaaaaa aatggtgtat tttaataatg
      961 ttttcattat cataattttt ttagctgcgc taatatctat ctatcaattt atttttttga
     1021 tttcttatca tgtcccaaca ggaacgccag gattacttgc aagacaaggt tttattgaac
     1081 atcggttatt tggtgtctat acaagtccaa atgttggagc tatgtttgga tatacttcta
     1141 ttattttaag tttaattagt gtattaattt caaaaataac aaaaacgatt cgtgtttttt
     1201 atatgatgaa ttttatcgta caaatatttt atattacact ttcttcgtct agaggtgccc
     1261 aggtagtaat ttgttctttt ttagtattat ttcttttgtt gtttgccaat tcaaaattta
     1321 gaaagagcat tagagaagta ctacctgtaa aaatcaaatg gatggtaagc atattattag
     1381 ttctattggt atatttttct tttggtacta attatgctaa ggatttttta gcaacaattc
     1441 ctgtatcgat tgatagaaaa gtagaagtta tagagtcaaa tgaatcagat aaaactccac
     1501 ttccagaaaa agaagtaata ttacagcatt ctagtgataa ctccgaaatt tcagctggtc
     1561 gttttacgat ttggaaagct gcgcttaaag ttataagacc aactttgtta tttggttatg
     1621 gagagaccga tttctatggg aatagcgatg caaaattttt gaaaaaagta gatcttgata
     1681 ctacagatgt gaatgaacta aaaagagcac atggaaacat gcataatgga tttcttcaag
     1741 tacttgtatc aagtggtata attgctttta tatgtatcta tgctttctat gtgttaaata
     1801 tctttagttt actcaaaaca ttttttagaa gtaaagtgaa ttatgcgttt cttggtattg
     1861 ttttaatttt tatactatct atttttatag atgaaatggt ggaggctcat gtacttttca
     1921 ataagcgcga tgtcatttca attgttttct ggtattattt gggaagcatt tcttacttgt
     1981 atttaaaaaa ggagaattaa tatgaagaca gctttcattg cttggactcc tttacatttg
     2041 attaatatat taaatgttta tcaaaatttt taccgagaat caaaaggaga tttatttatt
     2101 tattctgaat ttgaaaacgc tagaagatat agtgagcgta taaaagaatt agggctattt
     2161 aatgatgttg tttttgtgaa tcaccggaaa atcggttctt cctttaaacg taaaattaac
     2221 ttgttaacta accataatca atttttctcg gatgatttat ccgtttatga tgaaattttt
     2281 atacaggggg aaaattattt cacaaaaata cttttctcca atgtttacaa aaaaaatcca
     2341 gatgtaaaac taaattatat agaagatggt ttgggtactt atttgaagaa gagtatattt
     2401 tcaacaagta aaaacgttca aagagttttt aaaatattaa ataaaaatag catcttcaaa
     2461 aaaaaaattg acgattattt tgtctattgt ccggaattgt tactttatga tgtaaaaaat
     2521 ataaaaaaaa ttcccaaaat agatgaaaac agtaaattga tgaaaaacat acaatcaatc
     2581 tttgggatag aagaagtttt tgtcggagat aaatgtatct tttttgatca acctttatta
     2641 tctgatggtt atggcataga tgaaacagat attttcgcta aagtaaatag tatattttct
     2701 ggaaaaaaag aaactttaat taagcttcat cctagagcaa ggacaaatcg ttatggtgaa
     2761 aaaaacatat tgaattccag tttaccgttt gaagtcttgc tacttgaaaa tgacttctct
     2821 tcaacacttt tgattagtcc tatatctaca atttcttttt caccatctat gatgtttgaa
     2881 aagacgatca actctattat gttggctaaa ttaattttaa atgaatatac agcattatct
     2941 gaagataaaa aagaaacatt gacaaatatt gttcttttct gtgaacatta taaccgtcta
     3001 tcagaagaaa aaattttact cccagaaaac tgggcagaat taagaaagtt ggtagagtat
     3061 gggattattt aacacacttt ccaagtcaaa attagttaag tccagtttct ggtatactat
     3121 agggaatttt tttattaagg gaatttcatt cataactatt cctatttttg taaatattac
     3181 tactcaagca gaatatggtt tgattaataa tttcacttca tttgtatcaa ttttttcgat
     3241 tcttgttggg ctaagcctaa attcggcaat taataatgca agttttgact ataaagatga
     3301 cataaatgaa tttatgtcat cggtactttc tctagctaca ctttcgttaa ttgtgttctt
     3361 attaataggg aacatatatt ttttgttttc aaatgctttt tttgatatga accaacttgt
     3421 atttaatttg atgttattac aaagttttgc tagttttgta attagttata tccagtctta
     3481 ttttacgatt aactttaacc attttaaata tttgatttta tcttttatat ctactttttt
     3541 gaatattgca atctctgtag ctttgatgca gacattatat tttgatgata aatatgttgg
     3601 cagagtagtc ggcggaacac tgtcatttgt gttgataggt ttagtaattt atttaagaat
     3661 aatgataaag ggtgctcatt tttttgataa aaaacagtgg agatacgctt taaaaatctc
     3721 tttgccgcta atcccacatt ccttagcaaa tatagtttta gcacaattcg atagaattat
     3781 gattaattct tatttaggtc ctgctgcggg gggaatatat tcctacatta gtaacatagg
     3841 tattattcta agcgtaatat ggatgtctac taataacgcc tgggttccat ggttctatgg
     3901 tgagatgagt aaaaataatg aaaaaaaagt gaagaaagtt agtaactatt atttagtagt
     3961 ctttactttg attacgatga ttgtaatggt agtttcgatt gatttagcaa aatttatggc
     4021 tccgaaagac tattattctg ggttatcttt agtggttccg ataaccttag gctatttctt
     4081 tcagttttta tatagtttac cagttaatgc tgaattttat gagaaaaaaa catcgtttat
     4141 tgcgattggt acagtagcaa gtgctatagt taatatacta ttaaacatga ttttcatacc
     4201 taggttagga ataattgctg ctggatacac aaccgtattt acgtattttt tactttttat
     4261 ttttcattat aatatcgcta aaaaaattac gaataaacaa ttatttgaca caaaaaaaat
     4321 atgtatcata ataatttttg tatcaatttt ttctttgata cttcatattt taatcaatca
     4381 aatgttgatt cgttacgttt tgac
//
LOCUS       DARBFO010000145         4387 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000145 DARBFO010000000
VERSION     DARBFO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.150"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="SCK51_002796"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="SCK51_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HEG1253354.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3378)
                     /locus_tag="SCK51_002797"
     CDS             complement(2944..3378)
                     /locus_tag="SCK51_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253355.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3395..>4387)
                     /locus_tag="SCK51_002798"
     CDS             complement(3395..>4387)
                     /locus_tag="SCK51_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEG1253356.1"
                     /translation="LYMFKTRYYLFGPFRANNIDKVFFKLKMNNLNVAMADRERLYNS
                     LQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQN
                     YDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFE
                     KLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEH
                     LSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKIS
                     TAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT         1351 a          817 c          606 g         1613 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2641 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3361 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3421 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3481 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3541 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3601 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3661 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3721 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3781 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3841 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3901 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     3961 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4021 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4081 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4141 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4201 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4261 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4321 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4381 tgtatag
//
LOCUS       DARBFO010000146         4345 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000146 DARBFO010000000
VERSION     DARBFO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.151"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(387..1853)
                     /locus_tag="SCK51_002799"
     CDS             complement(387..1853)
                     /locus_tag="SCK51_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1253357.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="SCK51_002800"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="SCK51_002800"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEG1253358.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1412 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaa
//
LOCUS       DARBFO010000147         4324 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000147 DARBFO010000000
VERSION     DARBFO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.152"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..281)
                     /locus_tag="SCK51_002801"
     CDS             complement(<1..281)
                     /locus_tag="SCK51_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEG1253359.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..2138)
                     /locus_tag="SCK51_002802"
                     /pseudo
     CDS             complement(730..2138)
                     /locus_tag="SCK51_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2359..2721)
                     /locus_tag="SCK51_002803"
     CDS             complement(2359..2721)
                     /locus_tag="SCK51_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253360.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3063)
                     /locus_tag="SCK51_002804"
     CDS             complement(2725..3063)
                     /locus_tag="SCK51_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253361.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3042..3461)
                     /locus_tag="SCK51_002805"
     CDS             complement(3042..3461)
                     /locus_tag="SCK51_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253362.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="SCK51_002806"
     CDS             complement(3454..3687)
                     /locus_tag="SCK51_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253363.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4324)
                     /locus_tag="SCK51_002807"
     CDS             complement(3684..>4324)
                     /locus_tag="SCK51_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG1253364.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1223 a          961 c          620 g         1520 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aatcttattc
     4321 ggat
//
LOCUS       DARBFO010000148         4282 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000148 DARBFO010000000
VERSION     DARBFO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.153"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(37..1257)
                     /locus_tag="SCK51_002808"
     CDS             complement(37..1257)
                     /locus_tag="SCK51_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEG1253365.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="SCK51_002809"
     CDS             complement(1277..1888)
                     /locus_tag="SCK51_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG1253366.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="SCK51_002810"
     CDS             complement(1931..2863)
                     /locus_tag="SCK51_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEG1253367.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="SCK51_002811"
     CDS             complement(3284..4144)
                     /locus_tag="SCK51_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1253368.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DARBFO010000149         4084 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000149 DARBFO010000000
VERSION     DARBFO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.154"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(194..1699)
                     /locus_tag="SCK51_002812"
     CDS             complement(194..1699)
                     /locus_tag="SCK51_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1253369.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1696..2382)
                     /locus_tag="SCK51_002813"
     CDS             complement(1696..2382)
                     /locus_tag="SCK51_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1253370.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2575..3996)
                     /gene="gndA"
                     /locus_tag="SCK51_002814"
     CDS             complement(2575..3996)
                     /gene="gndA"
                     /locus_tag="SCK51_002814"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEG1253371.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1126 a          861 c          695 g         1402 t
ORIGIN      
        1 ttttaaacac gaatattaca gcattttatt gttttttcgt tcatctaaaa gatacttaca
       61 tttaattaac ggatgttatt ctatttaatt gttcgctagt ttttttataa tgacatccct
      121 gaatttctca cttgcttgta ttttgtacct tattctttgt tggatatata aaaaaacaag
      181 taaagcacat gctttacttg tcttctgcct ttgcgatagg aataaagatt ctaaaaattg
      241 tcccttgccc tactacactt tctgctaaga tacgtccttt atagttttca accagttgtt
      301 tagcgataga caaccccaga ccatttccac ccttcgttct agctcttgct ttatccactc
      361 gataaaaacg attgaagatc ttattaaggt cttctttggg tattccttcg ccaaagtcct
      421 gaatcgcgat ctcaaattca ttcagagtgg aagaaataga gatatggact tcttttcgtg
      481 tagttgaata ctttactgcg ttatccaata tgataatgat taattgttca aagtgattac
      541 gataaatttg cagggtcact tcttttgtca aatcatcatc taaagtgatc acatagtctg
      601 gatataatat cttgaaatta ttataaactt gataggtgac ttcttttcct ttagttgttt
      661 cattcgcata atgaacatct acttgttctg ctcttgataa gtctagcatc tcttgaacta
      721 agcttttcat acggctgatt tcttgcatgc tggcgcttaa tgattcttct aatatctcag
      781 gatcatcttt cccccaacga tttagcagac taagatgccc ttcaataatt gcaacaggtg
      841 tccttaactc atgagatacg tcttccacga attgctcttg ttgttcaata tacaaacgca
      901 tacggtcaag catctcattg aatatttcag ctaaatctgc caattcatca tttgtgtcga
      961 tttcaggcat gtggatatct gattgcggat ctttacgtat agtatccatt gtatctctga
     1021 gtaccttgag tggttttaag aaataagaag ataaaaagaa acctaaaata ctacttaaga
     1081 tcaaagaaac aacttctaaa acaataagag tcaataatag cttatttcga atatcataga
     1141 aagaagataa ttcatagaag ctttgcgcat agccgatctt ttctcttgta cctttagaat
     1201 atataggctg gatagataaa aagccagttt ttccttctag tgtaacgatc gttggaactt
     1261 tacgtgtagt ctgaatcagg tttcgactgt tttcatgggt tttaaaaatc aagttctcgt
     1321 ccaaatcata aacataaaga tccattgatg gctgccctag ctcagctata aaggaatcga
     1381 tcttcattaa gtttccttcg accagtgaag tatgatctac gacctcatct ccgtcatttg
     1441 tcgctttcgt cagattacga tacgtattta ctaatgtcag ttcttcatcc gagttagcca
     1501 aacgagaagc tacttctgaa atcgtacgct ctacattgtt tctttctttt gccacaatca
     1561 gattgataga tgatttatag gtaatgacag cgaaaattgt aaacacaacg aatatgaaga
     1621 aagaactcgc aaaggcccac ttgattgtca aggagggccc tttcagttct cttttttccg
     1681 attttttcgg ctttatcacg agcgcatcac ataacctgtt ccgcgtacag tttggatata
     1741 gctttcctcc ccgggaacgt caattttgtt gcgaagataa cgaatatata cgtccactac
     1801 gtttgtttca acttctgttt cgtatcccca cactttattt agtaaaacgt cacgtgctaa
     1861 taccacgttc acgttttcca ttaaagtaag cagcaactcg tattctcttt ttgttagttc
     1921 aattacttca tttccgcgac gtacgacacg gttttctttt tcgatagtca aatcgcgata
     1981 agtaatagta gtttgtttag ccacattttt atcgccttca atatcgattc gacgcaacaa
     2041 tgcacgaaga cgtgctaata attcttcgat agcaaatggt ttgacgatgt agtcatctgc
     2101 tccgtggtca agtccagata cacgatcgat cacagaatct cttgcagtca tcataatgat
     2161 tggtgtattt ttgacttggc gtacacgtct gcagacttct aaaccgttca attctgggag
     2221 catcagatcc aacaatatgg catcccactc attatttaat gccgcatcta aaccagttcg
     2281 gccgttgtag tggacttctg cgttataccc ttcatgcttc aactctagct cgacaaatcg
     2341 tgccaagttc ttttcatctt caatgattag aatattgctc atttattatt ttatcctttc
     2401 tattctactg gctttttaaa aactctatca ttataccgat aatatgctta aaaatctata
     2461 gtcattttat catggatagc aattttaaaa aagagctgaa gtctactcat aacaatcgac
     2521 ttcagctctt tcgtcctctt tttattttct catttcctaa cagccgaaag actattattc
     2581 ttcatggtac catgagtaat ggaagattcc ttctttgtcg acacgctcat aggtatgtgc
     2641 gccaaagtaa tcgcgttggg cttggattat atttgcaggc agacgttctg ctctgtatga
     2701 atcgaagtaa gcgatggctg atgagaaagt cggtacaggt acgccagctt ggacagccaa
     2761 ggcaacgacg tctctgactg attgctgata ttttttcgtg atttcaacga agtaatcatc
     2821 tagcaataaa ttttcgatct caggattttt gtcataggca tccgtaattt tttgcaagaa
     2881 tcgagctcgg atgatacatc cagcgcgcca gatttttgcg atttcaccaa atggcaagtc
     2941 ccaatcaaat tcttttgaag cgacacgtaa ttgagcaaat ccttgggcat aactcatgat
     3001 tttactgaaa tacaacgctt ctcggatttt ttcgaccaat tcttttttgt ctcccgtgaa
     3061 ctcaaaatcg ttcgtacgag acaaaatctt gcttgcttgt acgcgttctt ctttataagc
     3121 agaaatatag cgggcaaata cagattcagt gatcaatgga agtggcacac ctaagtcaag
     3181 cgcgctttgg cttgtccatt tacctgttcc tttattccca gctgcatcaa ggatcacatc
     3241 tactactggt ttacctgttc cttcatcgtc tttacgtgtc agaatgtcag cagtgatttc
     3301 tatcaaatag ctgtctaatt cgccttgatt ccattcttta aagatatcag ccatttcttc
     3361 tactgaaagg tttaaaatat tcttcattaa gtcataagat tcagcaatca gttgcatatc
     3421 tccatactca ataccgttgt gtaccatttt cacataatgt ccggctccat ttgggccaat
     3481 atatgtgaca catggtgctc cgtcttctgc ttttgcagaa atttgctcta agattggtgc
     3541 aactagcgca taggcttctt tttgtccacc aggcatgatt gaaggtcctt ttaaagctcc
     3601 ttcttcacca ccggaaacgc ctgtaccgat aaagttgatg ccagagttgg ctaactcttc
     3661 attacgacgc atcgtatctt taaagaatgt atttcctccg tcaatcaaaa tatcgccttt
     3721 atcaaggtga ggaagtaact cttggattgt tgcatctgtt gccggtcctg ctttgaccat
     3781 tagcaagatc cgacgtggtt tctcgataga ttctacaaat tcttcgattg tatatgttgc
     3841 tttgaaattt ttatctgggt gttcttctac tacagcggtt gttttagccc ctgtacgatt
     3901 gaataaagcc actgaatagc cgcggctttc tatgttcaag gctagatttt tacccataac
     3961 ggccataccg acaacgccga tttgttgttt tgacatatga actcctccta catacgggat
     4021 ttctccctaa tctcttacat tatagcttaa cacggctaca ttgcaaacta aagaacaaca
     4081 tatt
//
LOCUS       DARBFO010000150         4036 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000150 DARBFO010000000
VERSION     DARBFO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.155"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(242..391)
                     /locus_tag="SCK51_002815"
     CDS             complement(242..391)
                     /locus_tag="SCK51_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253372.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            853..1065
                     /locus_tag="SCK51_002816"
     CDS             853..1065
                     /locus_tag="SCK51_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253373.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1343..3142)
                     /locus_tag="SCK51_002817"
     CDS             complement(1343..3142)
                     /locus_tag="SCK51_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEG1253374.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3266..3862)
                     /locus_tag="SCK51_002818"
     CDS             complement(3266..3862)
                     /locus_tag="SCK51_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG1253375.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          689 g         1187 t
ORIGIN      
        1 atattaaata ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt
       61 cataaaaaac gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag
      121 agagacaatt tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt
      181 acaaacgtct tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc
      241 cttatctaga ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt
      301 tattttcgtt ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc
      361 ggtattgcat atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta
      421 gttcttgaaa gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt
      481 tgatgcaatt aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct
      541 attttctttt aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat
      601 ataaatcata tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga
      661 acaacatagg aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata
      721 gttatctaaa aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt
      781 tcaaatacaa ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa
      841 agagggaatg aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga
      901 gtatctcatg aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat
      961 gttgaaagtt atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc
     1021 gtaaaattct gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga
     1081 attcgcaaga actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa
     1141 aaagaaaaag ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta
     1201 ttttagctat tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg
     1261 tcaaacaaaa aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac
     1321 ttttttcatt aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg
     1381 aataagtgat ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg
     1441 ttatgatacc atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg
     1501 tccatacata tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg
     1561 tactatattc cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag
     1621 gtaagatgta gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt
     1681 acgtcaaact ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa
     1741 atcgttcgac aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt
     1801 ggtttagctt atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc
     1861 cgttttcagt aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat
     1921 ttacccctgt taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg
     1981 tttcctgaac atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg
     2041 cttctaaagt attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg
     2101 gttttgtctg aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga
     2161 aaaaaactgc aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg
     2221 cttgtgggat aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa
     2281 caatgatata aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta
     2341 aaagcgtcat agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca
     2401 tactttgtgc caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc
     2461 caatcacaat aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt
     2521 cccgaatggc tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca
     2581 gtcgaatccc aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc
     2641 aacgattagc aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat
     2701 cagaattaaa acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca
     2761 aaaaagtaat gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca
     2821 aagagattgg ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga
     2881 aataaactaa cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat
     2941 gttgagaaaa aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta
     3001 aaataaaacg ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca
     3061 tcagcaagac atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta
     3121 agctacgttt caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat
     3181 gaaaaaggaa agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc
     3241 aatgactaat tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat
     3301 aaatcaaggc catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg
     3361 tatatcgttt cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag
     3421 caactggaag aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc
     3481 agccaatata tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat
     3541 aacgacgccc tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga
     3601 taaatccagc ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa
     3661 tgattggaat aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga
     3721 tggaatcacc tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa
     3781 aataaatcgt cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt
     3841 ttcgagaggc agcaatattc attattcatc aactccttag aggttctttg ttaactatac
     3901 tttactagaa aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg
     3961 taagttcatc ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt
     4021 aataacttgt tttttt
//
LOCUS       DARBFO010000151         3923 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000151 DARBFO010000000
VERSION     DARBFO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.156"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(573..1130)
                     /locus_tag="SCK51_002819"
     CDS             complement(573..1130)
                     /locus_tag="SCK51_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253376.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="SCK51_002820"
     CDS             complement(1309..1920)
                     /locus_tag="SCK51_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253377.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="SCK51_002821"
     CDS             complement(2033..2290)
                     /locus_tag="SCK51_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253378.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="SCK51_002822"
     CDS             complement(2887..3165)
                     /locus_tag="SCK51_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253379.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="SCK51_002823"
     CDS             complement(3199..3450)
                     /locus_tag="SCK51_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253380.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1194 a          799 c          520 g         1410 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgt
//
LOCUS       DARBFO010000152         3876 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000152 DARBFO010000000
VERSION     DARBFO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.157"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            158..2257
                     /locus_tag="SCK51_002824"
     CDS             158..2257
                     /locus_tag="SCK51_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEG1253381.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="SCK51_002825"
     CDS             2453..3316
                     /locus_tag="SCK51_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEG1253382.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3876
                     /locus_tag="SCK51_002826"
     CDS             3421..>3876
                     /locus_tag="SCK51_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG1253383.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         1544 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa gaaaaa
//
LOCUS       DARBFO010000153         3870 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000153 DARBFO010000000
VERSION     DARBFO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.158"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            619..1659
                     /locus_tag="SCK51_002827"
     CDS             619..1659
                     /locus_tag="SCK51_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG1253384.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2483..2815
                     /locus_tag="SCK51_002828"
     CDS             2483..2815
                     /locus_tag="SCK51_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEG1253385.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3434..3775
                     /locus_tag="SCK51_002829"
     CDS             3434..3775
                     /locus_tag="SCK51_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HEG1253386.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1429 a          497 c          706 g         1238 t
ORIGIN      
        1 agaacggtag tacttaatat aaatataggt agtatctaat ttaagtatta atggtagtat
       61 ctaaattaac aggtagtatg taactacttt aaagtaattt ttttcagatt aacacagtag
      121 tatcttatta aataagttaa ttttaatggt agtacataat tattatatat atctgacatt
      181 ggtagtactt attgtatttt ttagtagtat ataatctatt gttttctagc ctaaagcctt
      241 gtcttataag aataatataa tatatactac tgaaataagg tagtatatat tactagtaat
      301 ggtagtatgt tttatatgca taaggtagtt cttaatttat tttttgtttt gacactggta
      361 gtacttaatt atctttaatt ttaaaactaa attttattca tacgtttttc tggtagtatt
      421 taattataaa taatatttat ttaaagtagt tcttaatgac tttaaaggta gtacataatt
      481 cattttattt ctgattttag gtagtattta attaaaaaac agttttaatg cgtttgcatt
      541 ttttatttat ctttgctatt ttcgaaaaga gtaggtaatt ttacataaat aaaacaacgt
      601 gaaagtagag gatctacaat gactgatttt aaatttttta aggcggatag agtttacaac
      661 gaattatttt atcaatttcc aaaagtcttt attgtttctg acgaatacaa aaaaatgaaa
      721 gattcaacta agattgccta tatgctttta aaagcaagat tagagatcgc aatcagcaaa
      781 cggcaaatcg atgaagaagg taatgtttat tttacttata cgacaaatga actatgtaga
      841 gtattaaact gccaaaaaca aaaagcgata gcaatcaaaa aagagttgga atcctttggt
      901 ttattattac aaaagcagat gggatttaac aaacagttag ggaaaaataa tcctaataga
      961 ctatatctag cagaattaaa agtctcagaa aatgatatct acttactcga aaaatttgat
     1021 agagagaata gggaaaacgt tgataaatca gagggtatga aaatcatacc caccctcgac
     1081 gaaaaatcag acgctgaatc ccttggggct caagagggta tgaaaatcat accgtgccaa
     1141 aacgttgata aatcagaggg tatgaaaatc ataccagaac ttaataataa tatattagac
     1201 actaatagac acaatataga cactgaaaaa gaccgcctac aagatcaatt gttgttagac
     1261 aattttgaga caattatgac aaacgacagc attgctacgt ttgtccctga acgagtatta
     1321 aatttgataa aaacattttc ttcaagttac agtgaagctc aaaaaaccgt ccagactatt
     1381 cataatgcaa agaaaaaagc tgaaatagaa agtggtattt cgatagtttt tgaagaactc
     1441 gatagttatt atgtcaatgc agaacaagaa ttatacacga cactgttaaa agcctatcaa
     1501 aaattaaaaa ccgaaaaagt cgaaaatatc cagaacctga tttttgtcta tgtaaaaaat
     1561 tggtttatcg aaaaaccaat agctgctaaa gtatcaagtg aaaaacgttt gaattatgaa
     1621 agctccccaa gcactattac gaaagactgg ttagagtgaa actttactga caaagtgatt
     1681 gttgcttcat ggagtaacat taaaattaaa aattttttat tattctaatt atattacgaa
     1741 tattacaaaa taatagctat tttaatagaa tttataagtt aaaatattat gataaaaatc
     1801 acaaacgtag ccttttacgt cataagtgtt aaaagaaata attaatttca aatatagaaa
     1861 agggaacgta tgtttgcata ctcctttttt ttgagttata ataatacgcg aagagagata
     1921 atttcattat ttaaacaagc taaaaatctt gatattactt cttttcaggc aatacgaata
     1981 ttattcgctt acattttttg tataaattta attgtctgct atgcttgtga tgaattgatt
     2041 ttcgttctgc acttttggat gataaatttg gacaattgaa aatacaaaaa aaagaaaggc
     2101 ggtatgaact cggaaggctg gtaaccttcg tcagcgttca tacacggcat taagatttgg
     2161 cccttaatgc gttaaccttt cagtgttacg gtattacccg caatggattt agtataacag
     2221 atattcaaaa gtgataccac tttttttggc tgtgttaagg ttaaaaatac gtgatattac
     2281 gatgaatcta tgcctaagca caatgttata aaaatgtccg tgtggctctt tgccgtatga
     2341 cgaacgctga aattagaggg aacgcatgtt tggatgcgtt ccctcttttt tgtttgtcat
     2401 gaacgaaaat caattctcta tttttgaaaa ataacaagaa aaacttgtta aaaatattta
     2461 aagaaattgg gtggtttatt ttatgtcata tacaggaatt ttgagtctca aagatatttg
     2521 tcactacggt aaacggtgta ccgctacaga gaaaatcacg aagaaactat caactggcca
     2581 aagtaaaacc gttgtgcagt gtaagaaata tataatccag aaagacaaag tctcagagga
     2641 aatgatttat tacattggga aacaaaagca aataatttta aaagatccta tccccttaaa
     2701 agagctatat ccaacaatca aacatgttta tgaccaaaac ggtgtactga ttggtcgaag
     2761 aaaaaatgga gtcttgcgtt gtaccgcaaa agggatgggg cgtttgatcg gctgaattga
     2821 tcattctcat aacttgtatt ttatcgtttt tcgcttgctt gaaaatattg tcgagacttc
     2881 taaaggaact tagagtcact agaaaaataa ggagtagact tgatctatca acaattccct
     2941 tcggggagtg acggggtagg ttttaacgat tggtaacgta aaaaattggt caatcgagta
     3001 aatgcgcttg tttggatagt ggtgaaatga atttgtgtaa ataataagcg cagagtaaac
     3061 aagagggaat ttagaagtta gacaagttag aatagcgtct agattaatca tgagcagcta
     3121 ctaagggatc tagactttga cgcaaactca tggaataagg agtaggtctt ctcgtaatgg
     3181 ggaggacgca caagaaattg tgtcgtgtgc tgcagtacat gtagatactt gcgaggacgt
     3241 tgattattgg atcaatcgag catttaccgc tgtctactag ggtttctcgt ttcggacgcc
     3301 ataaacgagt cgaaattttt tatgtatttt taagtaaacg aaaaacgata aaatatgaac
     3361 aagtcctgaa taaagtgaaa aaattgatat aaaacataga gaaaatgaaa tttacgtaat
     3421 ggaggaatga caaatgaaaa attatgatcc gaatatacga ttgggcacac atactattaa
     3481 ggtttctttc caacgttggg attacaaagg atttgtgacg tttagaagag gcggaaattg
     3541 taaaggatta gatgttttag cattagacga ggatgattta tacgatcaaa cattgactga
     3601 taatccaatt ggattcggct tgctacctga agatgatgaa ggtaatgaat ggtttaaaat
     3661 gacgcttatg aatgataatg gagacgaatt atctgttgaa gatacttggt catatttgag
     3721 tgattatatt gtcagctttg aaatcattga atttgtagcg gataaagaag aataaaaact
     3781 ggaggaataa attttacaga taaaggctta gaaggagtca cgatgattaa taaaaaggta
     3841 gtattacctt aggcattgag taaggaattt
//
LOCUS       DARBFO010000154         3713 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000154 DARBFO010000000
VERSION     DARBFO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.159"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="SCK51_002830"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="SCK51_002830"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEG1253387.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="SCK51_002831"
     CDS             complement(2261..2512)
                     /locus_tag="SCK51_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEG1253388.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="SCK51_002832"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="SCK51_002832"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEG1253389.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="SCK51_002833"
     CDS             3462..3680
                     /locus_tag="SCK51_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253390.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          797 c          688 g         1203 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aag
//
LOCUS       DARBFO010000155         3493 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000155 DARBFO010000000
VERSION     DARBFO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.160"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(82..378)
                     /locus_tag="SCK51_002834"
     CDS             complement(82..378)
                     /locus_tag="SCK51_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HEG1253391.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            733..1089
                     /locus_tag="SCK51_002835"
     CDS             733..1089
                     /locus_tag="SCK51_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1253392.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1099..1998
                     /locus_tag="SCK51_002836"
     CDS             1099..1998
                     /locus_tag="SCK51_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEG1253393.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(2395..3474)
                     /locus_tag="SCK51_002837"
     CDS             complement(2395..3474)
                     /locus_tag="SCK51_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1253394.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          959 a          737 c          766 g         1031 t
ORIGIN      
        1 agttgtcaca atctcttcta aacaatgttc aatcagctga ccttcggtat tagctcaaag
       61 cactgatttc acaacctcta tttaggcatg tgtctcagca agcggttgat agttgatgat
      121 ctgcattttt cgataaaaat cggcgggaag cttactccac cgttttaata attgatagaa
      181 ataactctct tctgctttga ttttatactc gcttaatatc aatccaaaac tattcgtttt
      241 ttttgaagct aattttttat aaatatccaa tgctttggtt ttatcttcat tgacaaagag
      301 tgtttttact tcaatataag aaccctcgat attttgtgtt tcttcgataa aatagatgtt
      361 ttttatatat tccttcatag tgattcctcc aaagaagtct tttatttgat aagtttactc
      421 taccgaagag ttgttaataa tccgtgaaca aaaagtaatt ctttttccaa tagttgttaa
      481 aggtttttta ttttttatag tattttttgt tatttaagaa aggaataagg ttaaacaaat
      541 aacaggttgc tttttgggtc tttttccgtt caagaaactg aacgaagaaa agggaaagtt
      601 gtctcttttg tgataagaga aatcataata aacgaatgac ttactttcca ataaattgag
      661 aacagtcaga accagacaaa ttagttgcat ctgatgaata aagtgattat tctatgacat
      721 gaggtgatac agatgaaatt agaagtgaca aaagaagcac aagaagtatt gaagaataaa
      781 ttagaagcag acgatcaact tttattagat atcgaaaacg gtgacggtcc atttgcagaa
      841 agccaagtca gttgccaatt ggatacggca tttcgattga tcattgtcaa aaaagataca
      901 caaacagatc taagtcttta ttctgtagtg gctgatacac cagttggacc aatcaaaatc
      961 aaagacagtg caatacttta catggatgat ccatccacac ttgctttaga accaacttat
     1021 aatagtttac aattaaaagg accaagcggc ttgagaaaag gcaacttgca agttgttcga
     1081 aaagactaga gggataaaat gggaaaattg gcgatcatca gcgacctaca tgtggacatc
     1141 aataaattcg gcgaatcaga gctgatgcag ctttggcaag ttttgcaaaa gcacaagatc
     1201 actcggcttc atctagccgg agatacggct aataaagtag accgttgcct agctgttgtc
     1261 gaatttttcg tcaataaagg attatctacg acatttcatt tcggcaatca cgagttggca
     1321 gatgtgccca atgaacagat gatggaccat tttccagacc cacatttttt aaatgaacgg
     1381 tatattgctt taaatgagca gaccgttctt ttagggatga atggctggta cgactatcag
     1441 ttttccgaat tgaaagatta cgaggcaatc gtacgaatga agcgtttgta ttggtatgat
     1501 cggttcatcc agcgtgggaa aacagatctc gaggtcaatg cagaaatgct tgcagctgca
     1561 aaaaatttgc tagaccagct ggaagcaaaa aaacttgagg tgattttatc cactcatttc
     1621 gttcctaaac gggcatttat catgtatcaa tctgctcctt atgagcgttg gaacaaatta
     1681 aatgcatttt taggatcaga atcattcgga gaattacttg accgttattc aaatatcaaa
     1741 caagtagttt ttggccatac gcatcgtcgc tttgaagata aatccatcgg tcacacgatt
     1801 tacagctgca gaccttttgg ttattattat gaatggcagc tgacccgaga ttttgtcttg
     1861 aaagaacagt tgctagacag ctatgatcct atgaaattac gtgtcctctt gcgtgcccat
     1921 cacccagctt ttcaggccta tcaaacacaa catttgcaag aggaatttga acaagcgatg
     1981 acaatcgttc cgtattgaga cagcattagt aatgtgtaaa agaaaatacc ttgtaaaaca
     2041 taaggataag taagagcagt agatccattt atcgtggata tgctgctttt tttgcggaaa
     2101 ataatcagcg aatgtttatt tgatcggatg atttgtaact cgcagagtac tagatgcaag
     2161 cgtgtattct ttttttgaac gataatcaca aaaaaaaata taatcgtaaa tttcaagttt
     2221 aagttagcca cccatggtga cccggttgct cgttaccacc aaccgtctaa tttttctaat
     2281 cgagtgggcc cttgacaatg agctgaggca tggtagtctc ccgactggct ggccccggcc
     2341 agctttccag acacaagcca tgccgaggga ggctcgttgt caagggccca cgtgtcaagc
     2401 cgtcaaccgt tcctgcctaa atgcctggac gaacgtgtca tgcggcgttg catagcccaa
     2461 aatctttcgc gggtaatcgt tcatccaacg ctgaatccgc aagatttggt tttcgctcac
     2521 gttgccgatt gggctgccct tggggatgaa acggcgaatc agcttgtgtt ggttttcgct
     2581 cgtcccgcgc tcccaggaag cataggggtg gctgaagtag atgtttgcca catcctgcaa
     2641 ggcatcgtgc agtcccgcga actccgagcc gttgtccgag gtaaccgtct tgaacagctg
     2701 gccgaagtac gcaccagccc gttctctcag ctgtcggacc gcttggttga ccgattcggc
     2761 atccttcccg gccagcttca gaatgacctc gaagcgtgtc tgccgttcca ccaacgtcag
     2821 cagcacggca tccgacttgg tcttgtttcc tacgaccgtg tcgacttccc aatggccgaa
     2881 ggttttccgg gtaccaaccg cctccgggcg ctcttcaatg gatttcccta gcacccgttt
     2941 attcgggcgg ttgccatgac gggacgattt cggtttgcgg gacagttttt ctagcaggtc
     3001 catattcttc gttttcatga tgccacggtc gatccactga tagagggtgg tggtcccagg
     3061 gataatggcc gggtcgaaca attcgtgttt tctggcaaag cccaccacca cgtccgggga
     3121 ccatttttcc aacagcatca tgtcgtccgc ccattcaatg aaggcgtccg tgtcggccca
     3181 taagggccgt ctgccgcagt tcaagcggtt cttctcgtag gccgcctgcc cggcatcggc
     3241 gtcgtagacg tcatgataat agtcgtagac cttcccgttc tgattctggc ggttaagctg
     3301 agtaacggtc ccgcgtttga tttcgttgtt tatggtctgc ggcacgcagc ccaggacatc
     3361 cgcaacctga cggttggaat agttttcctt tttcaggatg gcgatttggc aacgttctga
     3421 gtaggttagg tgttttccct tgcgtgaaac tgtgttatca tggttttgcg tcatgtgaat
     3481 tcatccttgt cta
//
LOCUS       DARBFO010000156         3487 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000156 DARBFO010000000
VERSION     DARBFO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.161"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="SCK51_002838"
     CDS             complement(<1..2232)
                     /locus_tag="SCK51_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253395.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="SCK51_002839"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="SCK51_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          982 a          772 c          548 g         1185 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg aatccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacact tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaat
//
LOCUS       DARBFO010000157         3444 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000157 DARBFO010000000
VERSION     DARBFO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.162"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            577..909
                     /locus_tag="SCK51_002840"
                     /pseudo
     CDS             577..909
                     /locus_tag="SCK51_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
     gene            923..1072
                     /locus_tag="SCK51_002841"
     CDS             923..1072
                     /locus_tag="SCK51_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253396.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1069..1515
                     /locus_tag="SCK51_002842"
     CDS             1069..1515
                     /locus_tag="SCK51_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253397.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1517..1654
                     /locus_tag="SCK51_002843"
     CDS             1517..1654
                     /locus_tag="SCK51_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEG1253398.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            1691..1984
                     /locus_tag="SCK51_002844"
     CDS             1691..1984
                     /locus_tag="SCK51_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253399.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1981..2205
                     /locus_tag="SCK51_002845"
     CDS             1981..2205
                     /locus_tag="SCK51_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEG1253400.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2202..3221
                     /locus_tag="SCK51_002846"
     CDS             2202..3221
                     /locus_tag="SCK51_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEG1253401.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT         1194 a          615 c          717 g          918 t
ORIGIN      
        1 ttactagatg acccttactg ggtaggtaaa acatcgttgg gtgagaatat tgcaaataaa
       61 ggtacgacgt tcccaattaa atctagcgcg tacgaaatat ataaaggtaa cacggaagaa
      121 gagcttataa taggtcaaac gtatactatc acgcttaaag gaacaaaacc cgcaagtcaa
      181 acctttgtag catataatta ttggaacgtt aattttggag aactaaaacc agttgaggga
      241 ttgacagacg tatggtctct aacattcaca ccaacgaaac ttgaaccggg tttgcctaaa
      301 gaccttcgca tttttcagtt gcctaaagaa acggccggcg catgccaaat tgactggctc
      361 aaaattgaaa aaggcgacac ccgaaccccg aatattagtc agtttaaata ctttggtgaa
      421 ggattgaaag atagcaacaa tccgaacgac tacagctggg acatcacgcc tgaatatact
      481 gaaaaaggct tgaatgatac ggttagtttg actgaaccgc aatccatcga aggattgaaa
      541 aatttcgaag atggtattca atcaaaagga aaatctgtat tgacatcaga cgacaacaaa
      601 tatgaagtcg taaccttaac agttacaaac gggaataccg gatcagcaaa gctttatcgt
      661 gaaggaaaaa ccgtcactat ttattttttt gcgttgaatg ggaaaagcag tggtggaaat
      721 gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg
      781 gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga
      841 gccattaaat ggcgaagaaa ctcaagttat ggatcagctt attcatttgt tatcacttat
      901 tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc cttacggatt
      961 cgagcaaatc attgtgaatg acgaagaaca tttaccggaa gaatgcacag aagtcgaacc
     1021 accgattcca aattggaaac cgaaattcaa ttactgggag ggaaataaat gaaaaacatt
     1081 tggaaatatg gccgtactgg cggagagtat gcaggaaaag tattggacga catgcttgta
     1141 tccgttcctt acacagatca gccaccgctc gaaggggttc gttcagatgg cgaaccgcta
     1201 acgattgctg atcagatgtt tgatcctaaa ttgaaccaat ggattatttt agcgaacgcg
     1261 ttagatcaca acgatttaaa caatctcaaa gcgatgtacg aggctcttga acatgaaaac
     1321 ggcaacctaa aacagctaaa tgccaaactc atgctaagcg atgtagcgat taaacaggaa
     1381 aatactgcat tgaaagaaaa agctgacagt ttagcacaaa tcaattcaaa aatgatgctt
     1441 gcttcgttac aaaatagcaa agacatttca gaaattaaag agcaactaaa tccagcttca
     1501 aaaggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg
     1561 ttttactgac gatcaagttc gactattcgt tccactatgc attacagacg aagaagcaga
     1621 taaaatcatt aataaagata agagcgcatc ttaattgatg cgtttttatt taaggtaaag
     1681 gagttgtcac atgattaatt taggggaatg gggaatgata gcaggatcaa taaccgctat
     1741 cgtttctttg attttattag taattagacc gattgctgca tctttctcga aaattactga
     1801 gactctttca aaagtaagcc acaatttaga tttgctgact aaagatttag aatcgagcaa
     1861 atcagatcga ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca
     1921 tacagaaaaa ttggtggaac acacgcaaca aatcaaaact ttgtttaggg agagaagaaa
     1981 atgaataata aaacgttcga agtactaaaa tggttcgcac tggtaattat tcccgcacta
     2041 gctactttcg tggggttagt tggtaaagcg ctcaattggc agtacacaga tatctgtgtt
     2101 gtcatcatta ctggttttgg cgcgttttta gggagtgtgt tgggtgtatc aaatcgaacc
     2161 tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca aatgaaaaag aaaattacta
     2221 ttactgcgat gagcctgtta acggctcttt ttttattgcc aattaacgga tttgcctata
     2281 ctatcaacaa tgaatttaat ttgggcgcaa atgaaggtag ctcacaagta gcaaataatc
     2341 agtatatttt actgcatgaa acggctaatg aaacagcaac aggacgcaat gaagcgcagt
     2401 atatgcaacg ttcatggact agcgcttata ctgcttatat tgtgggagac ggcggaattg
     2461 tttaccaagt cggacaacct ggttatgtac agtacggtgc tggttcatat gctaatgcca
     2521 atagtcctgt gcagattgag ttacaacaca cacatgataa agcaactttt gaaaaaaact
     2581 ataaagcata cgttgaattg gctagagatt cagcaatgaa atatggtatt ccattaacat
     2641 tagacacgcc ttataaccaa ccaggaatca aatcacattt atgggtaact caaaacattt
     2701 ggggcgatca cactgatccg tatggatact tatcagaaat gggtgtaagt aaggaaaaac
     2761 tggcttacga tttagctcat ggatttacag acgaaaaccc aacgacttct gagaacaagc
     2821 ctgtcattga tccaacccga gcaggtgcag caaatccaac tttatcagat ggaacgaacc
     2881 attctcacat tgatcagttt ggagaaatcg aaaatgcgaa cttacacgtc gctggatggc
     2941 acatcgctaa ctataagtac gagtatattt tcattatgga ctacaatact gggaaagaat
     3001 tagctcgagt aagagctgat ggaatttata gaccggatgt aaatcaagct tataatactt
     3061 ctggaaatgt tggctatcat gtatctttta acatgcgtaa cttccctaat aagaaagtct
     3121 atgtcatgat gcgggcaacg aatgatccag agggaaacac taaaggcggt gcgcaagatt
     3181 tccatgacaa acgttggtat ttaaatattc ctaaacgata aaaatagctc ctcgttgagg
     3241 agcattacat aactatattg acaactataa aaatcattcg ataaaatagt gatgttatcg
     3301 catatcttca ctatcaccca taatagtcac actccaagct atgcgataac aggtttgttg
     3361 ccacacattc tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc
     3421 tcagttggtc agagcagacg gctc
//
LOCUS       DARBFO010000158         3422 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000158 DARBFO010000000
VERSION     DARBFO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.163"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..788
                     /locus_tag="SCK51_002847"
     CDS             <1..788
                     /locus_tag="SCK51_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HEG1253402.1"
                     /translation="FYQKSYWGYSLPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIF
                     EMMDEEKKVSYDKQYIEDLYYKYMETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDE
                     VERIKEFVTKEEVVIISVNFEYSHIDIDYVFLSNLRRFHELPKESRTKCIVTSNIQAS
                     DVYLRTSYKKLLSEREVVRDNAGLMAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQS
                     MEIITKFALLDAMNVQMRDELSELAKKVKVTFLTDPRWVRIDK"
     gene            923..1501
                     /locus_tag="SCK51_002848"
     CDS             923..1501
                     /locus_tag="SCK51_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253403.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            1447..1965
                     /locus_tag="SCK51_002849"
     CDS             1447..1965
                     /locus_tag="SCK51_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253404.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            1973..3013
                     /locus_tag="SCK51_002850"
     CDS             1973..3013
                     /locus_tag="SCK51_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEG1253405.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            3262..>3422
                     /locus_tag="SCK51_002851"
     CDS             3262..>3422
                     /locus_tag="SCK51_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1253406.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         1234 a          383 c          678 g         1127 t
ORIGIN      
        1 gtttctatca gaagagttat tggggatatt cattaccaaa ctatatttca gcagttcata
       61 atgctcatcc taattatgca agctatctgt catataagaa tactttaaca attgaagcaa
      121 tgaatgaaat attcgaaatg atggatgaag aaaaaaaggt atcttatgat aaacaatata
      181 tagaagattt atactacaaa tatatggaaa ctgggaaaat acaggaggaa tatatggaag
      241 aactgagaaa atttctctca gacaaaaaaa ttctactgat tgcaccagga aaaagttctg
      301 tagatgaggt agaaagaata aaagagtttg ttaccaaaga agaagtcgta ataattagtg
      361 taaattttga atattctcat atagatatag attacgtatt tttaagtaat ttaagacgtt
      421 ttcatgaatt accgaaagaa agcagaacta aatgtattgt tacatcaaat atacaagcat
      481 cggatgtata tttacggacg agttataaaa agttgttatc tgaaagggaa gttgtaagag
      541 acaatgcagg attaatggca attagatttt tagccgatat gaatgtggat gaaatattct
      601 tagctggatt tgatggatat tcacataatg aagatgagaa ttatggtgag caatccatgg
      661 aaattataac aaaatttgca ttattagatg caatgaacgt gcaaatgaga gatgaattgt
      721 cagaattagc taaaaaagta aaggtgacat ttttaacgga tccacgttgg gttcgaattg
      781 ataagtagta gtaataatgt ttttaaaatt ttgataaaga gatagtgaga gaataaaaat
      841 gggtaaagtc ttaaataaaa aagaagaaaa atatttaagg gacgatgggg cgagatgaaa
      901 ctctaatttt gaacttttaa gaatcatttc tatattgtgt attgttgctt accatgctaa
      961 tttgcgttta aattataatg cagacggaat tatagtacat ggttcattaa ctgctaacca
     1021 gattttcttg aattatattg gtatttgggg tcagctagga gttaattgtt ttatatttat
     1081 ttcggcatat tttttaagtg agaaaagaaa cattcacttt gaaaatgttt tgggaattgt
     1141 attagaatgt attttttata gtgtattagt ttatggaatt ctttgtgcat gtggaataga
     1201 aacattttca ttgaacagct ttgtaaaaga aatattatca tttgctctag gtagatattg
     1261 gtttattaca tcctatttag cattgtatat tctaagtccg tatttaaata tcatgcttaa
     1321 caatctggaa aaacttcagt tgcaaaaaat aagtatgtta ttgtttatag gtgtatgctg
     1381 ttatcgtatg ttatttaaaa catctttgtt tggggattgt gctgttttaa ttgcactata
     1441 tgtcatatgt tatacactga aaaaaacgat aataatattt ttgagagaca cagaatcata
     1501 ggaacaattg tatgtataac agttatacca cttctcatat ccataggaat acttttcaaa
     1561 tttataccaa atgcaatggt taaccagatg gttcatattg gttctccatt gatggttata
     1621 gcaagtatat ttttatttta tgtatttaaa aatttttcat tttacagtaa gtatataaat
     1681 aaacttgcga gaaccagttt aggggtatat attttacatg aaaatcatta tttcattgat
     1741 ggtatgaagt ctagaggctt aatatgggat gggatatttg gattaggcaa aaggttttta
     1801 gatagggatt tttggatttg gtttataatt gcagttgtag ttgtgtgcat aatttgcatg
     1861 gctatcgatg ttgtaagaga aaaatttgaa agacagattt ttaaaattaa gagtgtaaag
     1921 aacctgtgta aacttatgaa tcatgttata aatatatacc cataattaat aaatgaggag
     1981 aaaagaagtg aaagttatat cattcggaga attattattg agacttgcag cacccggtta
     2041 ttcaaagtta tttcaaagag atagtttgga gactacattc tgcggagggg aagctaatgt
     2101 agcggtatcc ttagcaatat ttggatatca atcagaattt ataacaaagc ttccggattc
     2161 agatgttgga tatgcggcag ctaatgcttt gcgttatttt ggagtaaatg ttgataatat
     2221 tatatttgga gagggacgca tgggactgta ttatttagaa aaaggagcag ctcagagacc
     2281 atctaaagta atttatgaca gaaaattttc tgcaatttcg ttagctgata gtgatgaatt
     2341 taagtggaag gagatcctaa acgatgcaaa ttggtttcat tggacaggga taaatccagc
     2401 attgtctgag aatctctttg agacgtcctt agatgcatgt caggctgcca aaaaaagagg
     2461 tataacaatt tcttgtgatt taaattatcg atccaagctt tggacatcag aggaagcaaa
     2521 agaaaagatg gaacagatac taccattggt tgatatttgt atttgtaatg aagaagatgc
     2581 agaaaaagtg ctaggaatta aagctgagca tacaaatatt aatagtggag aattaaataa
     2641 agatggctat gtgcaggtgg ctagggagat tcacaagaag tttggatgta ggtatgtagc
     2701 tacaactctg cgtagaagtt attcggcaag tagaaatggt tggagtgcaa tgttatacga
     2761 agcagaaaaa gataaagcgt atttttccac agaatacaac atacaaattg ttgatcgcgt
     2821 tggtggtgga gacagctttt ctgctggtat aatatatgcg ttatgtgaaa atatgtccgg
     2881 acaaaaggct attgaatttg cgactgcatc tagttgttta aaacatacta tagaaggtga
     2941 ttttaatcga actacaattg aggatgtata taatttaatg aatagcggtg gaaatggtcg
     3001 cgtgttgcgt tagtcaacat ataaagataa atcacagtaa tttttatggt gattatctgt
     3061 cttagtagtg attagaataa cgtgaaaata aattgttgag cgtttatact gttttgaaaa
     3121 ataaatttaa catattgaaa aatacaaata tatacgtata ttataaagcg ccgattgtaa
     3181 aattaagcta gacaactaaa acagtcattt gtgctacact caaaatagtt ccgaccaaag
     3241 aattataagg agtgagtgca aataacctat acctatctta caacggatga acttgtaata
     3301 atagagtctt atttgaaaat taaccaatct gttgctaaaa ctgcgcattg cttgaatcgt
     3361 tcaagtcaaa cgatccataa agtatatctg ttcttcaagc aaagaaaatc agccttagaa
     3421 ta
//
LOCUS       DARBFO010000159         3263 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000159 DARBFO010000000
VERSION     DARBFO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.164"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            63..434
                     /locus_tag="SCK51_002852"
     CDS             63..434
                     /locus_tag="SCK51_002852"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG1253407.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
     gene            614..1036
                     /locus_tag="SCK51_002853"
     CDS             614..1036
                     /locus_tag="SCK51_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253408.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            1181..1426
                     /locus_tag="SCK51_002854"
                     /pseudo
     CDS             1181..1426
                     /locus_tag="SCK51_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1535..2491
                     /locus_tag="SCK51_002855"
     CDS             1535..2491
                     /locus_tag="SCK51_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253409.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
BASE COUNT         1029 a          536 c          708 g          990 t
ORIGIN      
        1 tctttttttt gctgtattca tttagattat gatagagaaa atacgtgaga gggggacctg
       61 ggatgtggct atctgatgaa aaaattcagt ggcgtagcta tcagaagcgt atattgacta
      121 actttgctac ttatttaaat aacaattcag tccatctagt ggctccacca ggggctggca
      181 agaccttttt aggaattgag atgatccgac gtttggattg taaaacctta attttggttc
      241 cttccctaat attgaagaaa cagtggttaa caacaatcga ggaatatttc ttagtaaatc
      301 cagcagatag ttgctatctt tctggggcga ttgaacatcc tagtcatatt acagtggaaa
      361 cgtatcagac catttacaac cgactgaagg aggagccaac ttatggtgat cagctggagc
      421 tgttagtttt ttgatgaagc tcatcattta aaaaaaagct ggggtgaatt gttgattgac
      481 ttgaagaaaa aacaaccgaa tctgttgacg atttccttga cagccacgcc tccgattgat
      541 agctctcaga cggagtggaa gcaatatatt gagttaaaat ggcctgattg acgaagaaat
      601 tagcattccc gagttggtaa aggaggaggt tctatgtcct catcaggatt ttctatattt
      661 gattccggct aatcaggaaa ctactcagca ttattttacc tttttagaga aacaaaatga
      721 gctggtggaa gcgttaatgg aaagccaaga gacggtggat tatttgcttt ctttagaatt
      781 tgttcagcat ccgcttgagc attcgaagga aatttatgaa aattttgagc tgttcactag
      841 tgcattaatt tacttgaatc atcataacta tgaattgata cccgaacatt ggcagctatt
      901 aggcagttct aagcagcaag gtaaaaaagc ttcagttcca tctttaggta aaaaagagct
      961 gatcactatt tatcagcaga tgtttgaaga tcaaccggaa ttgcctattt tttatcaatt
     1021 gaaaaaagct ggatgattgt ttgatgagtc gctgcatttg tttccagagt atccagacac
     1081 tgttggtagt caggcgattc ctgagaaaaa gcaagcagtt tgtcaaattt tgttaaaaga
     1141 agaagttcag ctgaagcaag aactgacagc ggtcattttg atggatcgta tttgcaagga
     1201 ggtattagca ggcagtgatg gtaccttagc ttatggcgtg gttcctttgt ttgatgaagt
     1261 tgttcgtcta ttaggtaaca ctcagcaaat cggtgccatc tgtggagagt tcttactgtt
     1321 accgaaaatg ctagtaactg ttgagctggc agaactagcg acgggtatga atgaacatcc
     1381 ttatatgtcg ggttattttt atttgacggt ggatgacaaa aaccgccaag cattgatgca
     1441 agcagttact cgcctattaa ccgaaaagaa gttgaatatt ttggtaggaa ccgttagttt
     1501 gctaggagaa ggctgggatt gtcctgcaat taatctgttg atcttggcta atcaagcttc
     1561 cacttttgtg caaacacaac aactacgagg gcggggctta cgcctaggtg cacaaaataa
     1621 agtaacgaac atttggcatt tagcgattgg attaccggga gttcctctgt ctgaacagcc
     1681 tgatttgaaa agaatgctac ggcgtttggg caatatttca ggtctgacct ttgaatcttc
     1741 accaaaaatt gagagtggtg gcgatcgctt tgctttacca ctggaatata cgaccaccac
     1801 gatggaaact tatactcagt cgatgttcta tttttcttgt gaacgaagtc ggttgaaaga
     1861 acaatggagt actgctttag ctgatggctg gcacctatct atgccaatcg tcgttaggaa
     1921 agtcgatcag gtaaagcaaa aacaagaaac ttcattgccg atagtagtaa aaaatataga
     1981 gcctctaagc ttctggtcag ctatatttaa aggtaacttg ctggaatatt tccaaattcg
     2041 taggcgacaa aagctatggg aaaaggaatg tgcccgcaaa aaaagcttgg ccatatgcgt
     2101 tcaaacgacg ttgcagcgcc ttggtgaagt tagtgataga aaagtaatcg tagcggctga
     2161 tcagcaggaa tttagatgct atcttgagaa tgctggctat cgagaaggac agctttttac
     2221 tgaagctatg agggagcttt tagcaccgct ggagaatccg cggtatttaa tcgaagggaa
     2281 aaagatagtt ctgacggtac cagaacgctt tggccgaaat aagcaaatcg ctaacctttt
     2341 tctaaacgaa ttaaatcaat cgtcgctgcc caaatgtcag ttggtgtata ctagaaatcc
     2401 tgaaggacgc caaaagctag tgacttatcg aatgaggcaa gtcttccaga cccgcgatga
     2461 agtaattatt caaaaaaaca tttggacgta agttgatgaa aataaatttt gctagagtag
     2521 aacaagtccg agaattaagt tgctacttaa ctgataatgc caaggacagg agaatctttt
     2581 cttgacgact gaaagaaact atttgactta tataacggtg tgtgatgaat atttgcaagg
     2641 cattctctta aaaagtactc gtaatagttt ttttgaaaac aaaacagtac tttattaata
     2701 ggactgacag agcgatataa gaacatctgc agacagaggt gaatgctttt ctcatgatcg
     2761 atgaaacaat ggtacttcgg atattcttgc ttttagtttg aaccgttgtg attttctttg
     2821 attcctaaaa gtagttttat agaatgaaaa tcaagcatac ggaatgctcc tttgattgtg
     2881 ttgtggtgat tcaatctaat ggagcttcta tatgtttttt tatttgcaaa aagaaccgat
     2941 agaatatttt tatcagtctt agaaattata gagccaagaa atttcaagca cacataaaaa
     3001 cgcgtgtttt atgcatttta tgagcaaaaa taactaaaga ccattacgta aaacataggt
     3061 aaatcagcat tcaatctcaa attatgtgat caaatgttga tattatttga ttataagttt
     3121 tcttttgcta ttatcagtct gcaaataaac agattataga gtatgaatgt agatagttca
     3181 atctttgttt acgacctcac atgttttttt actcaagaga aagtaatata aagtgactat
     3241 atttgacaga actttacata aaa
//
LOCUS       DARBFO010000160         3225 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000160 DARBFO010000000
VERSION     DARBFO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.165"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(445..1059)
                     /locus_tag="SCK51_002856"
     CDS             complement(445..1059)
                     /locus_tag="SCK51_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1253410.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1665..2042)
                     /locus_tag="SCK51_002857"
     CDS             complement(1665..2042)
                     /locus_tag="SCK51_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253411.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2342..3223)
                     /gene="galU"
                     /locus_tag="SCK51_002858"
     CDS             complement(2342..3223)
                     /gene="galU"
                     /locus_tag="SCK51_002858"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEG1253412.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          980 a          660 c          498 g         1087 t
ORIGIN      
        1 tcaatttttg tgtattttat ttctattaga aaaaggtatc ctgttggaca attttgttat
       61 atcaggtaaa cttcattgca ataatgataa caaatgtact ataattactt tgtaaagagt
      121 tttggggaag actctttcat caacgtgtat taagagacaa gaaagaatcc atatggattc
      181 tttcttgtct ctttttccac tactttttat ttttctgaca ctacatattc ttataactca
      241 tttctcataa ctgtgaaaca acttataaaa ataacaaact ttaaacaaaa caagcttaaa
      301 atcaaagatg atccatctta aaataactaa aacaaaaaaa gtagtcgttg ttaatcctca
      361 ttaataatcg ttttcttttt ttcaaacgct ccatttttat gtggaacatt atttttttaa
      421 ggaactttgt caacatgtgg ataactattt tccacttgtg tcctggacta gctgttttgc
      481 tgtaaaatac aaaacttgct gcagcaaaaa gatcgttgca agagaggctg ttccgcagat
      541 cgctggatct agatttggca aagtcaagac tgcatcatgt aaattggcgt ggttgtccaa
      601 aaccaaatca gcaaattcaa ataatcgcaa accactgcta tgttgcgatt tgatacttcg
      661 tgacaaatca aatccagtaa caatgattaa tggatgacca ttttctttga tccactgagc
      721 tagttcgatg attgccgggt ttcttccttc attcgaaaga agaagaaaaa tgtcttctga
      781 tttgacagaa agctgatcaa taattgcctg tcctgcacct tctagcgttt catagacacc
      841 tccggctgga ttttcaatca atatacttgg tacaagatcc ggggtttgat cccaaaattc
      901 aaatgctaca ccacttagtg tgcggctagc atatatctgc aatctaccac cttgattaat
      961 ttgtttcgcg atccaagtgc cagcttgaag catcagcggc tcttctatcc ttaatttctc
     1021 ttccaattgg tgataatagt caaaataaca taaactcatt tatctctccc gcctagctat
     1081 acttgacatg aatagaaaca gctgcctaat ctaaggaagt acataaaaaa acagtagtta
     1141 gacagtaaca catgtggtct ttgctgtttc aaacacaaaa aaggcattga acgaatacgc
     1201 ccaatacctt gacatagcaa ataaaacaac gtgcagacca tggaaaatat ctacataggg
     1261 gtagaatttt caccctagat taggtacaat aaattaggtc gatgcactgc attgactatg
     1321 gcaaaatgat cggtcagggt gtatgtgtgt gactggtcat tccctgattc ttagcgccaa
     1381 ctaagggtca gggagccttt ttttattcag ctgtttctat ctgaatttat tgtaagtaat
     1441 ctcttatcca agtgcaagca agaaaataca aaaaaacatt ggtatgattt atatatatat
     1501 aaataacata aaaaaaggaa tggcttatcc cgatacaaca agcattcctt taaaaaagat
     1561 tcttttatat aagtgaaaaa agcttttttc caccaattaa ataaatctct catctcaata
     1621 gtttatccat tattttatct ttttttgcgt atacctacta aatattaccg tcttcttctc
     1681 gttcttctac tttgttcgag agaatctgta ctagaaaaag tagttgtatg tctacctttt
     1741 ctgcctatcc gagactttct tttcatatat gggatataaa tgattagata cagtacagca
     1801 aaaaataaag caaatacgca agcttcccaa ctacctaacg ccaattcaac catcaaggta
     1861 gcaaccccaa cggatgctgc ataccagata aaaatcagaa acgtttcttt ttttctgacc
     1921 atgcgaaaca tgataaatag actaataagc aatagtggca acacaaatat ccaattgctt
     1981 ccttccatca gccacttgag tatatcaagc agcgactgtg caacagcgac taattttccc
     2041 atagtgaaca cacacctttt tttataatta aaaaattcgc ctttttattc tactatgaat
     2101 cttctggctt ggctttcatt tctcttatgt aatcattttc ttttatacaa cttaaaaaaa
     2161 aattaaacca ttagcataat attttctgaa tctattcacg aaagtctttt tctatctttc
     2221 gttttccacg actcaactga tctagagtca aaaaactaaa acgacagttg cgcaaatgac
     2281 gcacaagctg ccgctttaat aacaaatagc ctttcattct cagcaggcca cttatttcca
     2341 gttaactttc tgttcttagc tttttactca actggataat atatgcactt aatgaagtcc
     2401 caatttccga atgttgaagt ccatattcaa tatttgtctt catatatcct aacttatcgc
     2461 ctacgtcgta tctttttcct ttaaactctc tcgcatagac cgcctctgtc tggttaagag
     2521 tctgaagtgc atccgttagc tgtatctcgt ttcctgctcc tggttcttga gtttctaata
     2581 tctcaaatat tttaggagtc agtagataac gtccgataat ggctaaatta cttggtgctt
     2641 ttcccatttc aggtttttct acaaaccctt ttactctgca acgacccttt tcgctatctg
     2701 ttttaggatc aataattccg tatttagatg tttcattttc tggtacacgc atgacggcaa
     2761 cagcggaagc ttgtgtttct tcataatcca tcatcaattg ttgagtcaat gggatatcat
     2821 ctttcatcaa atcatccccc agcatgacga caaacggttc atttcctata aatgcttttg
     2881 cttgaagcac ggcatccccc agtcctcttg gatgagattg acggatgaag tgaagattga
     2941 cttgagtcgt ttcttcaact aaagcgagta attctgtttt ttccttttcc cgcaaattgt
     3001 tttccaattc gatatttgca tcaaagtgat cttcaatggg tcttttttct tttcccgtta
     3061 cgatcaagat gtcttcgatt cccgaatcca atgcttcttc cacaataaac tggatcgttg
     3121 gtttatccac gattggaagc atttcttttg ccatggcttt tgttgccggc aaaaaacgtg
     3181 ttcctagccc agcggcagga atgactgctt ttttgacctt cactt
//
LOCUS       DARBFO010000161         3221 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000161 DARBFO010000000
VERSION     DARBFO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.166"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            301..585
                     /locus_tag="SCK51_002859"
     CDS             301..585
                     /locus_tag="SCK51_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253413.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="SCK51_002860"
     CDS             588..1004
                     /locus_tag="SCK51_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253414.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="SCK51_002861"
     CDS             complement(1141..1860)
                     /locus_tag="SCK51_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1253415.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2535..2630
                     /locus_tag="SCK51_002862"
     CDS             2535..2630
                     /locus_tag="SCK51_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG1253416.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..3177)
                     /locus_tag="SCK51_002863"
     CDS             complement(2932..3177)
                     /locus_tag="SCK51_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253417.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DARBFO010000162         3128 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000162 DARBFO010000000
VERSION     DARBFO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.167"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            103..1347
                     /locus_tag="SCK51_002864"
                     /pseudo
     CDS             103..1347
                     /locus_tag="SCK51_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="SCK51_002865"
     CDS             1360..1992
                     /locus_tag="SCK51_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1253418.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="SCK51_002866"
     CDS             2197..2424
                     /locus_tag="SCK51_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEG1253419.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="SCK51_002867"
     CDS             2493..2759
                     /locus_tag="SCK51_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG1253420.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="SCK51_002868"
     CDS             2749..3018
                     /locus_tag="SCK51_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG1253421.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DARBFO010000163         3054 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000163 DARBFO010000000
VERSION     DARBFO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.168"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..354)
                     /locus_tag="SCK51_002869"
                     /pseudo
     CDS             complement(1..354)
                     /locus_tag="SCK51_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(614..1162)
                     /locus_tag="SCK51_002870"
     CDS             complement(614..1162)
                     /locus_tag="SCK51_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253422.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1163..2020)
                     /locus_tag="SCK51_002871"
     CDS             complement(1163..2020)
                     /locus_tag="SCK51_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG1253423.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2306..2592)
                     /locus_tag="SCK51_002872"
                     /pseudo
     CDS             complement(2306..2592)
                     /locus_tag="SCK51_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2582..2911)
                     /locus_tag="SCK51_002873"
     CDS             complement(2582..2911)
                     /locus_tag="SCK51_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG1253424.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          893 a          620 c          384 g         1157 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat acttatttga gagtcatcta attggctcat
      241 aatcctttct ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa
      301 catactcacc ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat
      361 ctcctcctaa tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca
      421 gattatcata cacttgttaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat
      481 aaccgttcct atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc
      541 tctatttcac gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt
      601 tcttctttga ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt
      661 cggtatcttg ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg
      721 gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat
      781 cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca
      841 attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta
      901 acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg
      961 gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta
     1021 tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct
     1081 ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat
     1141 caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg tgttcttcca
     1201 attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac
     1261 ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt
     1321 tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt
     1381 cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat
     1441 ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca
     1501 tcgattttac ttgggtctgt aagacgacga ttgcccaatc cgcaatgtac atcgcggatt
     1561 tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata
     1621 atggttctag cttttctttc atatatttga attgggataa tgaatcatta tattggtctt
     1681 tgatccaatc atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga
     1741 ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat
     1801 tttcgtaaac aatcggttca ccttctccgc ctccttcttc gttagtgaag ttcatatgcg
     1861 ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt
     1921 ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc
     1981 cacctttaaa attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt
     2041 agcaatattc atttgatact attataacat atttattttt agttactacc aaaaaaaaga
     2101 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
     2161 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
     2221 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt
     2281 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
     2341 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
     2401 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
     2461 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
     2521 ctttcaatgg cttctctttt agctttttaa atacttaatg acatgttttg aaggaacgat
     2581 ttcataagcc aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg
     2641 tatcttcagc aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta
     2701 caagaatctc atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa
     2761 aatcataaaa ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact
     2821 ttgcttcaac ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg
     2881 aattgccttg ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa
     2941 ttacttagta aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg
     3001 taatgcgtcc ttgctgaata aatttcaacc acgtgttcat cttcatctac ctta
//
LOCUS       DARBFO010000164         3010 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000164 DARBFO010000000
VERSION     DARBFO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.169"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            174..356
                     /locus_tag="SCK51_002874"
     CDS             174..356
                     /locus_tag="SCK51_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEG1253425.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            459..743
                     /locus_tag="SCK51_002875"
     CDS             459..743
                     /locus_tag="SCK51_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253426.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            759..995
                     /locus_tag="SCK51_002876"
     CDS             759..995
                     /locus_tag="SCK51_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253427.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1017..1271
                     /locus_tag="SCK51_002877"
     CDS             1017..1271
                     /locus_tag="SCK51_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253428.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1353..1538
                     /locus_tag="SCK51_002878"
     CDS             1353..1538
                     /locus_tag="SCK51_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEG1253429.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1605..2000
                     /locus_tag="SCK51_002879"
     CDS             1605..2000
                     /locus_tag="SCK51_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEG1253430.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1990..2367
                     /locus_tag="SCK51_002880"
     CDS             1990..2367
                     /locus_tag="SCK51_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253431.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2389..2763
                     /locus_tag="SCK51_002881"
     CDS             2389..2763
                     /locus_tag="SCK51_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253432.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          922 t
ORIGIN      
        1 atgtttatgt atatgtatat gaatataaac gtattaggta cgaacacttt taaattcttt
       61 tctgggcttt gtttttaggg tagggctaaa aaaaacatca ccatctgatc caaaacagct
      121 cagaaacgca ttttaacgta tctgatacca atattacaga aggagtgtat cgtatgatta
      181 acctatttct tttagtgaca gctagtacat tattctttac tggaatatca ctgataattt
      241 cctatttgat ctataagaaa gccgaaaatc acacaagcaa agcaatcgga ctagccgttt
      301 catccttttt aactttgctg actttatttg ttactttatc gttttctttt gtttagaaca
      361 gctgatccat tagcaccttt ttctctttac attatacaca ctgttgtgta taatgtattt
      421 gtaaacaagc taattaactt ttaggaagga gtgacaagat gctaacagct actttacagc
      481 aacctactca cgttgctatt gcagaaagtt atacaattaa tgcagggatc gttaaacagc
      541 tgatcaagga tcgagaattc aaaaagttta acagtcggca agcgtataac agacagctaa
      601 agaaagctat tctaaagcac aaagattcct tcatcgggaa cttggaaagt gtagatgtta
      661 aaactgtttc aacggcattt tcagttgttg aagtgactat tgacggtcgc ttgatttctc
      721 tatttgcttt tggaattaat tgatagggag tgtgaacttt ggaaggtaag acttttgcag
      781 aaatttggac tgaatttatt gaggtaccaa ctcgcattga tccgataact tctgaaaagg
      841 tattggatgc agacttctac aatctcaaag caggtactag ccttgaatgc gtttggatgt
      901 atttagaaga gcatttcaaa ggcgtaacgc tcggtcacgt cttataccac aatggtgccg
      961 agtgggatgc aattggaaat atccattttt cttaatataa gaaaggagaa cacaatatga
     1021 attataccga tgaagaacaa atcgctttgg aatacgtttt cacaaatgaa gaaggggagg
     1081 aaattgaaaa agtcttgatc atttcatcaa aagacttgga gaaggctttg gcttatgaac
     1141 catacgaatc aaccgtaact gtcattgaat tcttagatac ctatacttct aatgatactt
     1201 gggaaattat gaatatttgc aacgctaata acattcaatt tgctgaaaaa accaataaat
     1261 cattttatta gttttgcaat aaaacatcct ctttttctat tgattccata cacaacaatg
     1321 tgtataatag attttgtaaa gggagggtag ctatgcctaa gacagcaaga gaaattgtaa
     1381 aaattctcaa atctaacggc ttcaaaaaga ctggacaaac tggcagtcat gcaaaatttt
     1441 ataattccaa gactaaaaga acggtcgttg ttccgatcca caatggtgat gtaccactag
     1501 gaacagaagc taatatctgg aagcaagcag ggctttaaca gccttgcctt cctatttggt
     1561 attaggcata ctatcctctc ttacactaga aaaggagcga ggaaatgaag acaaaaaata
     1621 ttttttatcc ggcaatcttc gagaaagaag gagaagctta taacatcgtt ttccctgact
     1681 taccggaaat atctaccttt ggaaatagtc ttgaagaagc atatttaaac gctaaggatg
     1741 ctttaggatt agcgctatat agtgtaccag atgcagaatt tcctaaacca tcagcagtgg
     1801 aagaattaac tttgaaagcc aaccaagtgg tagtaatcat ccaactaaac attaaacttt
     1861 tccgaagaag cttaaataca aaaacaatta gaaaaaatgt tagtgttccg gaatggttag
     1921 ttcttttagg gaaggagaaa aacataaact tctctcaact acttcaaaaa gcactagaag
     1981 aagagttgct tgaaaagtaa aaaggagacc gaacctatga aagctacaca cttaaactat
     2041 ttctctcgaa cgttaatcaa taatctatta aaagatacaa ctggcattag cgttagtacc
     2101 aaacactttg ccaatgatct aattattttt gaggataata acttgcgaat tgagatgttt
     2161 attgaacagg caacccctaa ctcaatacct gaattctgct atacgcacac tgaaaaaatg
     2221 aaacatgcaa aatcagttaa agatgttgaa gcattaagtg aatcactaac gaacgcttta
     2281 aacctcgtta agaaggcaaa attgttattt gaatttcaaa ccgactacca caatgagttg
     2341 cttgctcaat ctgaaatttt agattaattg aaacaggaga agagaaagat gaaacaaaca
     2401 caaacagaaa ccgttaacga tacgcttgct gatcaattag caggttatta taaattattt
     2461 gagatgggta aagcacagcg cttgatccac tatctttgga aatacaaaat tttgtttctt
     2521 ttttgggaat caacactaac attctcagtt cttttttata agtcagattt ttataatcaa
     2581 cacttagttt tagggagttt tttgcttatc tacaccattt tgatgcttaa tttatatacc
     2641 tacatcacac ttgaagaaca cttagtaatt agcgatgcag ggatcgagag aaacatcaaa
     2701 agggtatcct tcaagatcac gttaaacaag cctaccgata aaaagctctc agaagcgttc
     2761 tgagagcttt tgacattttc ttgtataatt ttatcctaac acattacaaa gcacctaatt
     2821 cgagcttata tcaagcaaat ttgctattta ttactcaata catatctaaa aattacttga
     2881 tggaaaatta gatacatggt aagcatgaaa agaaaatcaa tttgagcaga tgcagtacaa
     2941 tacatagcaa tttaaaagct ctcagaaggc ttctgagagc ttttaatata ttctttgata
     3001 atttgtcttt
//
LOCUS       DARBFO010000165         2927 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000165 DARBFO010000000
VERSION     DARBFO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.170"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(45..1364)
                     /locus_tag="SCK51_002882"
     CDS             complement(45..1364)
                     /locus_tag="SCK51_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1253433.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="SCK51_002883"
     CDS             complement(1361..2014)
                     /locus_tag="SCK51_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1253434.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..>2927
                     /locus_tag="SCK51_002884"
     CDS             2302..>2927
                     /locus_tag="SCK51_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1253435.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          959 a          477 c          323 g         1168 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgaca ccttctgttg
     2101 ccaatacttt ttcttttttc taataaatgc aaacagacac cttgaaatcc tttagaataa
     2161 agggttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tattgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaaactaa tcgctaagaa gtacttcgtc
     2701 tcggatggga cggtgacacg gatcttgcgt gaagcaacga agcattacca gccacgtatg
     2761 aacttccttc cgacggtgtt gtgtatggat gagttcaaat caatgaagtc tgtttctggt
     2821 tccatgagtt ttatttgtgt tgatgggaca acccatcagc tatttacgat ccttgaagat
     2881 cgcagacttt acaaactaac tcaatacttc ctgcgctttc ctagaaa
//
LOCUS       DARBFO010000166         2902 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000166 DARBFO010000000
VERSION     DARBFO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.171"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1304
                     /locus_tag="SCK51_002885"
     CDS             <1..1304
                     /locus_tag="SCK51_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1253436.1"
                     /translation="PGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADK
                     YEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVT
                     YPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTY
                     AWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLN
                     KEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVV
                     DKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLE
                     AGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQ
                     SSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1438..2781
                     /locus_tag="SCK51_002886"
     CDS             1438..2781
                     /locus_tag="SCK51_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1253437.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         1153 a          388 c          552 g          809 t
ORIGIN      
        1 ctcccggcgg tacaattgat accaacacac caggtaatta cgaaggtgtc attgaagtaa
       61 cgtatccaga cggtacgaaa gatacggtga aagttccagt agaagtgaca gacaatcgtt
      121 ctgacgccga taaatatgaa ccaacagtag aaggcgaaaa agtagaaatt ggaggcacag
      181 tagatttaac agataatgtt actaacttgc cagcgttacc agaaggaaca acagtaacag
      241 atgttactcc cggcggtaca attgatacca acacaccagg caattatgaa ggtgtcattg
      301 aagtaacgta tccagacggt acgaaagata cggtgaaagt tccagtagaa gtgacagaca
      361 atcgttctaa tgcagataaa tacaatcctg aaggacaaaa agtgactaca gacttaaata
      421 aagagccaga tgcatctgag ggaattaaga acaaagaaga tttaccaaaa ggaactactt
      481 atgcttggaa agagaaagta gatgttagca cagctggaaa taaaaaaggt actattgtag
      541 tgacatatcc agatggatca tctgatgaag ttgaagtaga cgttacagtg acagatactc
      601 gttccgatgc agataaatat aatcctgaag gacaaaaagt gactacagac ttaaataaag
      661 agccagatgc atctgaggga attaagaaca aagaagatct accaaaagga actacttata
      721 cttggaaaga aaaagtagat gttagtacag ctggaaacaa aaaaggtact gtagtagtaa
      781 catatccaga tggatctaaa gaagaagtag aagttactat ttctgtagta gataaaaaag
      841 caccgaataa acctcaagtt gatcctatta cagagggtga ccaaattgtt actggtaaaa
      901 ctgaaccaaa tgcagaggtg acagttacat taccagacgg aagtcaacac catgggacag
      961 ctgataaaaa tggtaatttt acagttaaag ttcctaaatt agaggcaggg acaaaagtga
     1021 tagtaactgc aactgatgaa tctggtaata ctagtgaacc tactaatgta gttgtttctt
     1081 ccaacaaaaa agacagtgga aaaaacggta gtaaaggtag taagactgac aatcaaggta
     1141 gcaacagcaa tcaagacaaa aatagaggca aaagccagtc ctcaaaagtt tttcctaaga
     1201 cgggagagag tgacagtaat atctttacta ttagcggtgg tttaatactg ttaggaacgt
     1261 taggattatt agggtataaa aatcgaaaaa aagagaatga atagaaaatc aatttcttaa
     1321 gtcttctctg agaagaggaa gaatcttttt cttcctcttt ttgctttttt atgatgggaa
     1381 ataagtgtta gttaaagatg aatatacttt ttatagaaag gaaaagaatg taattaagtg
     1441 gaaaaaatag taattattgg ttcaaaccat tcagggctag ccgcagctcg atttctaaac
     1501 ttaagcgatt caaattatga agttattcta atcgataaaa aatcagaaat tagttacttg
     1561 gaatgtggga catcgttatt tataaaagga gaagtaagta atttagaaga tcttttttat
     1621 caaaatagaa tgacactaaa ttctgagata aaaaagttat ttctagagac agaagttgac
     1681 aaaattgata ttcaaaataa aaagattagt tgtgtaaaaa aagatggtac aagattctct
     1741 caagaatatg acaaagttat attagctaca ggttcaaagc aaattatgct agatataccg
     1801 gggaataatt tagctaatat tttttcgata aagaattttc caaatgcaaa agagattttt
     1861 aaagctttgg atgatagtac tattaaaaaa gtaggaatta ttggagcagg atatattgga
     1921 gttgaaattg ctgaagccat aagaaaaaga gggaaagaag tatatctttt cgatgttgca
     1981 gatagagtgt tgtcgactta ttacgaccgt agtttttcag ataaagttga agaaatacta
     2041 tcaaaaaatg gaatacatct ttgtctatca gaaagtccta aaaaatattt aggaagaaaa
     2101 aagattgaaa aaatagtcac tacaagtggt acccaatact ctatggatat ggtcgtacaa
     2161 gctataggat ttctatctaa tagtccaatt ggggaaaagg aactcttgag agatgttagt
     2221 ggagcatatc taactaatga ataccagcaa acaaatataa aagatatata tgctattggg
     2281 gattgtgcta caactttttt tacgtccatt aatagaagta gtgttgattt ttctatttct
     2341 aatgctctaa gaacagctta tattgcaact caacatatta acaatcaaga ttttacacct
     2401 tctggttccc aatttacaaa tggcttttct atatttaatt acaattttta cgcagcaggt
     2461 ttgaatttaa aaacagcacg attattaagt gagtctatag attatataga aatagaagat
     2521 ttcattagac ctgctttttt gagaaaaaat agtcgtgtaa aattaagaat tagctttgaa
     2581 aaaaatacaa agagaatagt aggtgtacaa ctttgtagtc aagaagattt atctggacta
     2641 ttatcaatgt tttctcttgc tatagaagaa caaattacat tagacaaatt gaaaatgtta
     2701 gattatttat ttcatcctag ttttagccaa ccctataatt ttattttaaa ggcagtaaca
     2761 ggattaaaca ttgaaacata atcgttacta aatgaaatca agaaatatac tgtattactc
     2821 aattcattga aaatatttat cataattttt aatagaaaac actattaaag agaaaaaaga
     2881 gaggtaaatc aaatgaaaaa at
//
LOCUS       DARBFO010000167         2894 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000167 DARBFO010000000
VERSION     DARBFO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.172"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..124)
                     /locus_tag="SCK51_002887"
     CDS             complement(<1..124)
                     /locus_tag="SCK51_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HEG1253438.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNV"
     gene            194..544
                     /locus_tag="SCK51_002888"
     CDS             194..544
                     /locus_tag="SCK51_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1253439.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            958..1776
                     /locus_tag="SCK51_002889"
     CDS             958..1776
                     /locus_tag="SCK51_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1253440.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1849..2208)
                     /locus_tag="SCK51_002890"
     CDS             complement(1849..2208)
                     /locus_tag="SCK51_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1253441.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          981 a          409 c          517 g          987 t
ORIGIN      
        1 agacattgta aattgtttgg cgtccacgtt taagctgctt cgcaacgatg gcaaccggag
       61 tttcttgatg aaaatatgct tctatcatta caagctcgtt tggtgtaaga tgggtataag
      121 tcatttatgt tcactctcct tgtatgcttt agcgggtatt acaatttgag tgtaacataa
      181 atggcttttt tatttgtctc gcttaattat acaaacggcg ttttataaag caatcattcc
      241 tgagaactta gatgaggtta aactttattt atgggaagat aacggagaaa ttgtcggttt
      301 tagtggtgta agtgcagaag aattagttat gctatttctt gatccagatt ttattggtaa
      361 gcgatatggt agtcaaatat taattgagct gattgaaact gaagatataa aaagaattga
      421 tgttaatagt caaaataaat atgcaaaaca tttttattta agccatggat ttgaaattga
      481 atctgaagaa gacttagatg gttttggtaa accataccct ataacgcatt tagtaaaaaa
      541 ataattcatg aatggtttat tacctgattg aacatgaata aaagtaatat ttttattttg
      601 atggcctatt tctaggttat gaattatcca gatcaaattt gtgtattaca ttcagtaata
      661 aggtgcttat cttaggatca acttatgata ctagctgacc aaggatgtac cagttataga
      721 aaatcagtta aagtaaaaat gataaaaatc ttatgtcaac actgatttag gctgacctgt
      781 gggttaggag aaagacaggt cggtagcttt taatacaaaa aatggcttgt atgtatctag
      841 atagttaact gaaattagaa atgttcttat ttttaacgga gtaagaaaaa taggctgctt
      901 taggtctgaa tactttttca atataatgaa gatatcaaag gcgacaggag tgtttttatg
      961 aataagaatc ctcataatct aaagttggta gcagtagata tggatggaac atttgctcgt
     1021 actgattaca catacgatat cccaaggttc aaggctgttt tatcgcgaat gaaagctgtt
     1081 gattgtcaat ttgttgttgc aagtggaaat caatactacc aattgagatc gttatttcca
     1141 aattattatg atgaattaag ttttgttgct gaaaatggtg cttttgttaa agataaaaca
     1201 gaggtactat tttcagccga tatgcctaga aaagcgatcg aggtggtcct tgatttatgt
     1261 aaagactatc ctgaagtaaa aaatgtgatg tgtggtctga atagtgccta ctgtgaaaga
     1321 ggaaaggtga gtcaagcatt ttttgatcta accaatatct attaccatag attagaatgg
     1381 gtagctgatt ttgcagaagt tcaagataaa attttaaaat ttgctccgac tatgcctgtt
     1441 gaaaaaaccg aattttacgt tgaattgttc cgtgaaaaat tagccgggct attagtacct
     1501 accagtagtg gtcatggttc tgtagacttg attctttctg agtgccataa aactttcggc
     1561 ttgaaaatgt tagttgagcg cttggggatt aatcctgacc agtgcgttgc ttttggagat
     1621 gggggcaatg acattgagat gttggagtat tgtggtctga gttatgaaat ggataatgca
     1681 acggaagctg ttaagcaagt tgctaagcat caatgtcctt caaatgacga agacggtgtt
     1741 ttagtgacgc tcgatcgatt gttccctaac acgtaaacac taggtaattc atcaaaaaac
     1801 ctcgatataa gaaatattcg aggttttaaa actatattta gaacagattt acgaagcgtc
     1861 tgatgagtca aaatagactt ggacattgct atctaaaata ccactttcat tctttaaacg
     1921 aattgtttga tttgatggag ttacctctaa tactaaatgc ccattaataa aggtagtatc
     1981 gtattcagat gttttaactg ttgcttcttc gcaaggtatt gttaaatcat atgctggaat
     2041 ataatgttgt gtggaaacta ataaaaaatg atcaccaatc atacagcatc cctctgctga
     2101 agaaaattga ttagagccat catttaaagt taacaagaat ccatccttta aacgaggtac
     2161 ttgcttaaat acttctggtt gtacttctaa aaataaatta tcgctcattt tcacgaccac
     2221 catcgacttc cacagttgaa attgacattt tatttaacac acctattccc ccttaactat
     2281 aactcaattc tatataaaaa atattctatt ctcaaataat tgaacttttc aaatcgccaa
     2341 tttacttcgg ctttatcaga gctcattcat atcaagagcg attaacgctg taaaaattaa
     2401 accgaagatc tgataaattt tctctatttt ctacaatatt taaaacgctt ccttagtcta
     2461 ttatatatct atttctctta ggagtatgtt tagatgtttg aataatagtt gcccaacttt
     2521 aaaggttatt cttataatat tttacttcat tttccaatag aaaattcaaa ggattactgg
     2581 tttttctaaa atagtttagt ttaaatacat aagtatcctc cgacactgta ttagttcata
     2641 atcatctaaa tacaaaattt tcaaataaaa atatttcaag ctctatcgtt gacgaatgta
     2701 aacgatactt ataaagtaat agaaattgat tacatttgta aacttaaaat ggttaattta
     2761 tctcacataa aactaagaat aaaataatga ttaaaagtgg ttagttttta tacgatttag
     2821 cctatcggta aaaatgaatt agagagtgta aaatattttg tgtaaatgaa aaaatccata
     2881 caaaaaagga agtc
//
LOCUS       DARBFO010000168         2887 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000168 DARBFO010000000
VERSION     DARBFO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.173"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            799..1053
                     /locus_tag="SCK51_002891"
                     /pseudo
     CDS             799..1053
                     /locus_tag="SCK51_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1123..1635
                     /locus_tag="SCK51_002892"
     CDS             1123..1635
                     /locus_tag="SCK51_002892"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1253442.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1632..2669
                     /locus_tag="SCK51_002893"
     CDS             1632..2669
                     /locus_tag="SCK51_002893"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1253443.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1042 a          513 c          574 g          758 t
ORIGIN      
        1 attgatgacc acgaaagtgc atggcgatat ttgatgttga tactttatgc agtaccctat
       61 gtattcttcg ctatcattct gtggctcata gcattgtttt ttagagtatt taatgacctt
      121 taggtagatt tatatataaa aagggctaaa tacgcaaaat acccccttaa aatgcaaaat
      181 aaatgcaaat aagggggtaa ctccttatga aaaaacaact aatagtatta taacacgttt
      241 ttatgccgag aaaacttatt ggttggaatg ggctatgtgt tagctaactt gttagcgagt
      301 tggttggact tgaattggga ttaatcccaa gaaagtacca actcaacaac acataaagcc
      361 ctgtaggttc cgaccaataa ggaaattgga ataaagcaac gaaaggagtt gagtaaaatg
      421 agtgagaaaa cgaagaaaat cattcgagta tcattagctt tttcacaagt agtagtgcaa
      481 tcattgatag tttattatac ttaccagcaa tataaattat ctaaagagaa ttaagttttg
      541 attatgtaat aagttttggt actaaaaaag acttgatcta attagaccaa gtcttttgtt
      601 agtgttatat taataacaaa ataaaaataa gaagtcgctc actccccgac caaagtttgt
      661 gagcaaaata aacagtcgat gtttgtttct taattgtata tcttgatatt aaacgattta
      721 agattattct tcaagatata tattcgcaaa tgtaattgga aaagcgactt cttaaacaaa
      781 attaaggagt cgctttttat ggcaaaaata acgaaaaatg agcaagaaaa tttggtggaa
      841 aattacacaa aaaaaccggc gtactctaat agccggttag acgcacattt cgtgtgcact
      901 tctaatccga aattgagctt tgacgctatg acgatagtcg gaaaccttaa taaaaacagc
      961 gctaaaaagc tatccgattt tatgagttta gatccacaaa ttagactttg ggatatactt
     1021 caaacaaaat ttaaagctaa agcactacaa gaccccctat gttaggctag ttgtcgtata
     1081 ggataataag ttataataaa gactatacgg aggatgatta ccatgacaaa taaaaacaat
     1141 gaacgttact ctaccgaact gaaagcgaca gccatcaaga aaatgatgcc acccgaaaat
     1201 agggggattc gccaattaag tcaaactttg ggcattccag aagcgactct ttattcttgg
     1261 cgtaaaaaag ctagaattca agggaatact acacccggaa cgaaaaaaaa tgtcgaaaaa
     1321 tggtcaagtg aagataaatt tttaattgtg atggaatcat tctcaatgaa ccaagtagaa
     1381 cttgcggaat attgccgaaa gaagggctta tttaaagaac aaattgaggc ttggaagaaa
     1441 acgtgcttat ctgcgaatga acaggaagaa aatcgaacga gagaacttgc aactgaacta
     1501 aaagaagaga aaaaacaagc gcgtcaatta gagaaagaat tacgtcataa ggaaaaagca
     1561 ttagcggaag ccgctgctct cttattacta agaaaaaaag cacacgcgat ttggggggac
     1621 caagaggaag aatgatttca tcttcagatc gcaaacaagc tgttgaactt attcaagaag
     1681 ccaatcgcaa tggggcccgt ctcacctatg cctgtaatga gctgaatatt agcgttcgaa
     1741 cctacgaacg atggacgcgt gaaggtacga tagcgcatga tcaacgccct ttggcgaaac
     1801 gtcctgtacc caaaaataaa ctgacggatc aagaacgcga aaaaataata gaaaccgttt
     1861 caaaaaaaga atttatgaat ttgccaccta gccaaattgt accaaaactt gcggattgct
     1921 cgatttatat tgcatcagag tctagcttct atcgtgtatt gcgagaaaaa aatatgcaac
     1981 atcaccgtgg ccgaagtcaa gtttcacaaa aacgcatacc gccaagtcac cttgcgacga
     2041 agccaaatga agtttggact tgggatatta cttggctaaa aggtccaata aaaggactct
     2101 tttaccggtt atatctcatc attgatttat ttagccgtaa aatggtaggc tgggagattt
     2161 gggaatcaga agaagctcgt catgccgaaa aacttattaa acagacggtg cttaaggaac
     2221 gtatccaagg acagccgttg gttttacact ccgataatgg cagccctatg aaagccgcga
     2281 cttttcaagt cttactcgaa aaattaggaa ttcaaagttc ttattcaaga ccacgagtga
     2341 gtaatgacaa tccttattcg gaagcggctt tccgaacgtt aaaatatcga ccagaatatc
     2401 ccaataacgg ctttaaaaca atagatgatg ctcgaaaatg gacgaaagag tttgtcgatt
     2461 ggtataacga ttctcatttg catagtggga ttcaatttgt gtctcctgca gcgtgtcatg
     2521 aaggacgaca tctcgctatt ttagagaaac gaaagcaggt ttacgaggac gctaaaaagc
     2581 gccatcctga acgctggtca aaaggtgttc gaaattggac tccacatcaa tcggtggcgt
     2641 tgaatccttt aaaagaaaca gaaagttaac aggagcataa tctttcaaga ttatcaaata
     2701 accctcgtca cccaataggg gaaaaagtta ttcttcccct attggataac gaggccaacc
     2761 gaccataggg aggttctgct ttttgatagg tggaaaataa aaatacgaca actatattga
     2821 caaacaccga ccatcgtcca gtaaagagac gcaataaatt ctatcgaagt ttacgcactg
     2881 cctctac
//
LOCUS       DARBFO010000169         2848 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000169 DARBFO010000000
VERSION     DARBFO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.174"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(551..1303)
                     /locus_tag="SCK51_002894"
     CDS             complement(551..1303)
                     /locus_tag="SCK51_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1253444.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(1318..2565)
                     /locus_tag="SCK51_002895"
     CDS             complement(1318..2565)
                     /locus_tag="SCK51_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG1253445.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(2641..2787)
                     /locus_tag="SCK51_002896"
     CDS             complement(2641..2787)
                     /locus_tag="SCK51_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HEG1253446.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT          987 a          470 c          351 g         1040 t
ORIGIN      
        1 taaagtcaga taaaaattct ttggaaatgt aatggtagag atatttttac aaaaggactt
       61 gcagcgctgt agttggtgct gcaggaggac ctatttcggc cgttacagga ggtattggta
      121 atgtacttgt cggatgtgca ttaaagcctg ttcctgcaag ttaaatttat aaaaacagga
      181 aagtaattat ctcaaatcct atcaagtaca atcataggat tgcacttgac aggacaaatt
      241 ttttaactaa aaaatgaaaa acactttcta aaaattagta gaatttgcca aatctttcgc
      301 atcttccacc atacatttaa ttttaattcc cctttatggt acttaataaa aacaagaaaa
      361 gaactattca tttggccctt ttttggttcc agaccataga aatattcctc cagtaactaa
      421 tattgctgtt atgcacgtaa ctaataacag ctataaaaat tggtacaact ttaataggat
      481 tcagaaaaat ttgcactagt gaaggaatct attttattag aaaaatgcaa tctccctaaa
      541 gaaaaaacca ctaaacagtg ctttctttca acttctcctt aattttttgt gctttcccta
      601 aagtaaagtt tctggccaac tctgaatcaa aatatagtga acgtttttta aaatctattt
      661 ctttaacgtt aaagatattt actaaacacg ctcgactaat tcttgttaac ataggatatt
      721 tcttttcaag ctcactaatt cttccataaa attcgtactg cccatcttta gtacacaatg
      781 ataatctatg tggtaatttt gaagcttcta caaaataaat gtcatttata tcaaaagtaa
      841 atgtttgaga acctatggaa aaaacaaatg cttgattcgt aacctgtttt gttgcatcta
      901 tcctttcttg cgctaataac aataactcaa caatctcgtt tcgatactca tctagtgttt
      961 gatcttttgt tacaaatcct aacgtttcaa ctcttctttt tattgttacg ggtaacatct
     1021 catcatgagt agttgtaaaa atgatttttg cttgaacatc atattttctg attacttctg
     1081 ctaattctat accgttaact tcatgattta aatcaatatc taagaaatat attccttgct
     1141 ttggtcggaa ttgtttgagg tattttttaa cttctaacgg actttgtgtt ttcaatacta
     1201 tcttaaatac tttatcgtga aaaagaatga aattatcaat aattcgttct aactgattta
     1261 attgtataat ttgatcttca cataatatta ttggataact cataaatcct ccttccctca
     1321 aaacaacaaa ataacttgag tagtaaattg atacggatcg ttttgatagt tcacaaacat
     1381 attgggattc tttttattga tttcctctat actagataaa cctaaccctt gatgtccctt
     1441 ttttgttgtt attccttttt tcattagagt atctatagag agtagtatct ctctacaact
     1501 attcgatatc gaaatttcta tctgtttatc atctcggtaa atagctagag acagttctct
     1561 atcttgactc tctatagatg cttcaattgc attatcgaga ataattccaa gcaccctaat
     1621 acagtcaaat attggaacag gaacttcaat aatagtactt ggacattcga atttacaaca
     1681 aatcttattg ttatttgctt ggtatagctt cgctatcaac aaacttttca aatattcatt
     1741 ttttatattt tttaaataac gatattcaaa cttattggtt agataagaat tagaatagat
     1801 ctctaattct tttatttctt tttccaatat cgaatcatgg ttttgattag cgatttcttt
     1861 taaactcagc aataaatttt tatagtcatg tctaaatctg gcaaattttt cttgatcttt
     1921 ttccagtgaa tcagtatatt tttttagata aaccaattct tttcttttca attgctgttc
     1981 atattgttct cgttgcctaa tagttattct aataaataaa aatagaacaa caaacatttg
     2041 aatgattaaa aatattaaga caccaaacac aaaatgatca aatacttgat attgatgagc
     2101 aacataagaa acaaataaac caattgtaag taaataaatc atgaatatag cagtaagttt
     2161 agaactggac tgttcaacaa tcctatttat agctaatttt ctatataacc aaataaacat
     2221 cagcaaaaac aaagaatcaa gcattatctg gaatactaca taaccatacc cttttatccc
     2281 ttcaaatgaa aacatatacg tcataaccat ggaagtaata atagatctga aatcatcaat
     2341 aagcatacat aatagtaatt tatttaaaag cactatatta gttttcttct tttcctttaa
     2401 tataaaataa cttccgatta taggtatagc tgcggaaaca tcagtaaata attcaacaaa
     2461 aaaagtgagt ggtatcaata aacatacaaa aagatatttt cttttgttaa gatcagtatt
     2521 agtcatataa aataagagaa aaaagaaaaa gtttcccacg aacattctta atagataagc
     2581 ttttgtatcg atagtaatca agagatcccc tcctgatttt aggatatcat aaaaaaaatt
     2641 ttagttacac tttttaaata atgaaaaaac acattccact aaatttgatg ccggttttcc
     2701 ttgaggtttt gttccagcac ctcctttaat ttttttaagc tcttgatctg aacattgttt
     2761 agcatttagt ctattttttt cttccattac gattccccct acaaactata aatactttta
     2821 tgtcactgat tcaatatacc tatttttc
//
LOCUS       DARBFO010000170         2784 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000170 DARBFO010000000
VERSION     DARBFO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.175"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            30..215
                     /locus_tag="SCK51_002897"
     CDS             30..215
                     /locus_tag="SCK51_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1253447.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            227..475
                     /locus_tag="SCK51_002898"
     CDS             227..475
                     /locus_tag="SCK51_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253448.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            506..685
                     /locus_tag="SCK51_002899"
     CDS             506..685
                     /locus_tag="SCK51_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253449.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(728..1198)
                     /locus_tag="SCK51_002900"
     CDS             complement(728..1198)
                     /locus_tag="SCK51_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253450.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            1285..1983
                     /locus_tag="SCK51_002901"
     CDS             1285..1983
                     /locus_tag="SCK51_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEG1253451.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            2161..2502
                     /locus_tag="SCK51_002902"
     CDS             2161..2502
                     /locus_tag="SCK51_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253452.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            2495..>2784
                     /locus_tag="SCK51_002903"
     CDS             2495..>2784
                     /locus_tag="SCK51_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEG1253453.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         1024 a          401 c          563 g          796 t
ORIGIN      
        1 gattattttt ttagaaagga gctattttta tgtctgagaa tttagactta aaaattcgag
       61 cggagatgag aaaaagaaga atgactttca aagaactagc tgcgcttatc ggtatttcag
      121 gagcttattt atcagatatt ctaaacggca atcgtgatgg aaagaaagca caacagcata
      181 tcgaaacagt gaaaaaaata ttggacatcc gatagggggg taggcgatga caaaactaaa
      241 aaaacaagat tttgtaaaaa aatacaatta ttctccatct acttatcaac gtcgaatgtc
      301 ggaactaaaa aatacagcaa ttttctcagc ggcgtatgaa cgggtcacag gacaagaagt
      361 ttggatcaat acagaattat acgataaatt tttgtctttc aaatcctata acaggttacg
      421 cacaagaaag gtaacaccta aagaatttat cgagaagcat ttagttgatt tataaaaaac
      481 agaacatttt gagaggtgaa ggttaatggg taaattcaac agagcattgg tattcagcgc
      541 accgctaatc atctacgctt taggactttg gggaagcagg caagcgttga taggaacgat
      601 tgtttacatg gtttggattt ttatggggct tgatgaagct gaagctgagt acagagcgaa
      661 aaagccaacc gagggggctg actaaaaatg aatagaaaag agaaactaga atgtatatta
      721 ctattactca gtttaattct ttcactaatt tctctattgg gaagtttgta tttttgatat
      781 caacaatttt gtggatttgg gttctattta gagtaaatat catttttgtt tggtgattaa
      841 ggagtttatt aaaattaatt ttccctactt caaatgcaag gtaacaagat atagcacttt
      901 taggaggaat atttagcggt acagcttcag agtatattct attatcatcg actgatcctc
      961 ctttagcaat taatttctta aatggagtag ctgaccatct gcggtttaag ttagcggatg
     1021 aaagttctaa attaactaac atagctggtt ctgttgaata gttagaaatg attacttttg
     1081 tataaatcat atctgcagcg aagtaagaag cattaagttc aacttctagt tgagggcgca
     1141 ttccttttat ttttatccct gttaaaaacg tactcaatat aaaacttatt attgacatcc
     1201 attgaaaaat tgttaagtga aaaaaattca aaataaacac caccagtttt taactaaatt
     1261 ataccaaaaa ggagagaaga aataatgcaa gaattagtaa ttttgaaaaa taaagaagct
     1321 gtgactacga gcttacaagt ggcagaaagc ttcgagaaaa aacatcaaca tgttttaaga
     1381 gatattgatg cactaaaaaa agatgtgtcc aattttggac agatgtttgt agaaggtaat
     1441 gaaccagatt catatggcag aaatcgacga gttttcttca ttagtagaga tggatttttc
     1501 ttgctggcta tggggtttac aggaaagaaa gctatctact tcaaacaaaa atacattgaa
     1561 gcattcaacg aaatggaaga tgttattcgc aagaatactg ttcctcaaac aattgaagat
     1621 atgatgatct atcaattaga agaaatgaaa gatgttaaaa aagatgtttc catgcttaaa
     1681 gatactatgc gaattagcgg acaacaagag tttgaaatta agcaaaaagg aaatatgaaa
     1741 gttatgggag ttctaggggg aaaagaaagc cgagcttatg aagaaatcag caaaaaagta
     1801 ttctcaaaat tttggtctga atttaaacgt accttttcaa tcccaagata tggcgagtta
     1861 cctcgtaaga gattcgatga tgctgtttca tttattgaaa tgtggttgcc agaaactgcg
     1921 atccgtatgg aaattgatca actgaacaga caacagagac tttttggtga tgacaatgaa
     1981 tagagctgaa gcgctaagaa tagggacggc aattgctaat tgctggtgga aatactataa
     2041 accaatcatc ctaagccaac aacatattga caagcaaaaa gcatggcaac aaataaaaaa
     2101 gtgactccgc cggcaagcaa agagtcacaa agaaaacaca tcataaggag attttagcat
     2161 atggaaaaag aactttccac tctagatcaa tatttgattg atcctgattg gggcaagccg
     2221 aaaattgagg aaacaagtgg tcgaaaaatc agacgaaatc ttttgacgaa tgaagaacta
     2281 gcttgggatc aagatgattt aggcaaccat gtaactattt gggatcatgt ttatcttatc
     2341 catctatcga agcattcgaa taaacctgaa tatatttacg tcatcgaaga tggcttgatt
     2401 gatgcgctag aagagtacga cagagataac ttgattgata tctcttatta cggaccaggt
     2461 aagaaataca ttgctgaaat ggaggcagaa tttgatgagt gaaatcaaag ggacaacgaa
     2521 ctttgaaaaa ctttttagtc gtaagttaaa taaaattctc aagaaaaaag gaaattttga
     2581 ttatttatct tgggctcacg cgtgggagat tatgaaaaag aatgatccac aggcaacggt
     2641 aactattaat gagtacaaac actacagggt tgtttctgga actcatcaag actttcttgt
     2701 tgaggaatat aaaccttttc ttatggatga aactgggact tatgtatctg tctcagtaac
     2761 ggttaaagga cacacggaaa ccga
//
LOCUS       DARBFO010000171         2643 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000171 DARBFO010000000
VERSION     DARBFO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.176"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..2157
                     /locus_tag="SCK51_002904"
     CDS             <1..2157
                     /locus_tag="SCK51_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1253454.1"
                     /translation="RNYESEIFRFDNETNTIIGENDSGKTNALTALRILLDDSYYYSS
                     KTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNSESQTILEELIS
                     NADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDYEFYYTSKSNLN
                     FCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVYVDALRDVLREM
                     KNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIGENINRQLNSII
                     GSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIYLALKIVEFEAC
                     RSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVHLAEVSEISRMN
                     VLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNGLLFSKGVILVE
                     GDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRIQRKCAIVTDLD
                     EQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEIEFLDVNKENIL
                     TYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGWFAIELGEKIDF
                     LIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSASLKTSDTADLN
                     IAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2154..>2643
                     /locus_tag="SCK51_002905"
     CDS             2154..>2643
                     /locus_tag="SCK51_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEG1253455.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQV"
BASE COUNT         1001 a          356 c          442 g          844 t
ORIGIN      
        1 agaaattatg aatccgaaat ctttcgattc gataatgaga caaatactat tataggtgag
       61 aatgattcag ggaaaaccaa tgctcttact gcattgagaa tcttattaga tgatagttat
      121 tactatagta gtaaaacact aaaagaatca gatttttttc atggaattca aaatgggtgg
      181 caagggcatt ggattattat ctctgcgaca tttgaaggaa tcagcgaaga agaatttgac
      241 aatgagattt gtgcttccat ttctttaaat tctgaaagtc aaacaatctt agaagaattg
      301 atttcaaatg cagataaagg tgtaggtagt atttctctat ttattagacc taacaaagct
      361 attagaaagc aattatttga tatttccact gaattagact ctcatcaatt taacgaattt
      421 agaaattcta ttcgtctatc cgactatgaa ttttactaca catctaaatc aaatctaaat
      481 ttctgtatca atgaaaatta tgataaattg gtaggaagac tcaatgacaa tatagctagt
      541 aatcctgaag aagacgatga agctctgttg ggctcaagaa ttgacatgtc agatattttc
      601 aagcatattt cggttgttta tgttgacgct cttcgagatg ttcttagaga aatgaaaaac
      661 aatcgaaacc cagtcaaaag aatcatggaa acaattgaat caaaaatatc atcagataat
      721 gttgagtcat taaaaacaat tatacagcaa ttaaatgaga ctattaccag tgttcctgaa
      781 attaggaaaa ttggcgaaaa tattaatcgg caactaaata gcataattgg ttcagtgtat
      841 tctcctaatc ttcttctttc ctcaactatg tcagatgata tgggttcttt agcaaaattt
      901 ctttcaatga agccagaaca aaatatcgac ttagacttac tagggttagg acatctaaat
      961 atgatttatt tagctttgaa aatagtcgaa tttgaagcgt gtcgttcaag agaactactt
     1021 aatattatgt tgattgaaga accagaagct catattcatc atcatattca aaaaactctg
     1081 ttcgaaggat taaatcttca aaaaaactat actcagattc tcatgaccac acattctgta
     1141 catttagctg aagtgtctga aatatctcgg atgaatgtat taaaatcgtt taatggcaaa
     1201 tctatcgtta tgaatccagc aaataatctg aactcatttg ctaaagaaaa gttaaaaaag
     1261 aacaatctaa atctaataaa cgcagtagag agatatttag atgttaaaag aaatggattg
     1321 cttttttcta aaggtgttat cttagttgaa ggagatgctg aagaaatact tattcctcaa
     1381 atagtaaaaa aattatttgg tataaactta gacgaacttg gaataggttt gattaatgtt
     1441 ggtagtactt cgtttgaata tatcgctagc ttgtttgacg atgatagaat acagagaaag
     1501 tgtgctattg taactgattt agatgaacag gcaatcccat cagaacatcg attgtataaa
     1561 agcactgcac aagagaaagg tcaagcaaga aaagaaaaat tatcctccct ttttaatgaa
     1621 aacaattggg ttaattgttt ttatgctaat acaacatttg aaattgagtt tttagatgtg
     1681 aataaagaaa atattctaac ttatgtcagt ccaattatag atgacgcatt caaagataaa
     1741 aaaacaataa ctgcttataa agaagaaata caaagtgaac aatatttaga aagaaattta
     1801 gcgatgttaa aactagccaa tcatgttggt aaggggtggt ttgcaattga gttaggtgaa
     1861 aaaattgact ttttaatcaa aattcctaag tatattctta atgctattgc ttttgcatgt
     1921 caggaaatag tagatctctc aatttataaa aaaattctac tccatacaat cgacttgtac
     1981 gaggaaacta cagaattgtc agctttatca gctagtttaa aaacaagtga tacagctgat
     2041 ttgaatatag caataatttc tttattagag tactttggta aagatgagga tgttgtgtat
     2101 ctaatcaatc agatagaaaa ttacacaaaa atactttatg ctgaggtttc aaaatgacaa
     2161 atatcttcga aaaaatcgta aatgatttaa atttagaaca aaaagatgtt gtggaaagtg
     2221 aagataacct ttatgtaaat gcatgtcctg ggagtgggaa aacaagggta cttacaagga
     2281 aaattgctta ccaagcaatt aaacacgatg gatcactaaa aagaataatt gctatcacat
     2341 acactaatag agctgctgaa gaaattaagg aacgcctaag tttacttagt atagatgatg
     2401 atattaatat ttgggtgggg acaatacatc agttttgtct tgaatttatt atttatcctt
     2461 ttaaaatgaa tctctcaaga gtatcaaaag gatttacaat tattgatgat tatactcaaa
     2521 gagcttacgt cagccaaatt aataatatgc tgaatttaaa tctcaaaacg tatgaactga
     2581 acaaaattga tttgtcactt tctcatgatt ttaaaattaa tgaagttcag tatactcaag
     2641 ttt
//
LOCUS       DARBFO010000172         2566 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000172 DARBFO010000000
VERSION     DARBFO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.177"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..379)
                     /locus_tag="SCK51_002906"
     CDS             complement(<1..379)
                     /locus_tag="SCK51_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEG1253456.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPS"
     gene            598..1968
                     /locus_tag="SCK51_002907"
     CDS             598..1968
                     /locus_tag="SCK51_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HEG1253457.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1989..2273
                     /locus_tag="SCK51_002908"
     CDS             1989..2273
                     /locus_tag="SCK51_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253458.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          873 a          384 c          526 g          783 t
ORIGIN      
        1 gactaggaaa actatctata attccgacta gttcaccttt ggtgagcttc ttgatgaaaa
       61 agtatcgaat aatcttgata gtgtaagcta agtttgaact taagcgagtg aatcgactcc
      121 tactaggaaa gtcaccatta agaaataaga cggaacaaac cgctctatac gtggctcttt
      181 ggctagtata gccattaatt atgccccata taacacaagc aataatcact gtatcgcgtt
      241 gcttcagttg atcagtattt cttcggtttt taatcttatc tggtacacaa ttgtggtata
      301 tgttagaaac agttctcata atttccttga atgttataaa agtatctgta taatgttcag
      361 tatattttga tgaacgcata tgtagtcctc atttcttggg agttttgaga ctactatatg
      421 cgttttttgt tgatttacca acatgtttag acattttcct ctaggaaaaa attaactagc
      481 accacgcgta ttgtttatat taaattagat gaccgtgata atgcacagca aatttttgaa
      541 agtatcaatt ctaccggtga aagattgact gcctcggatt tgattcgaaa cttcatcatg
      601 atggataaat ctaatgaaga gcagactacg ctttatacta aatattggcg taggttggaa
      661 gaagtctttg ataattctaa aggaatggaa gactttttca gatactacct tgctgcaata
      721 actggggagt attcagcaaa gcatattctt tatcaagcat tcaaagacta ttggcataaa
      781 gagcgcgatg tatcaagtga tgccgaattg ctccaaaaat tagtgcgata tgctggttat
      841 tttgcgagac tttactataa caaacctgaa ggaaaatact cagaagtatt gagcgatttc
      901 caaagtatgg aaagtatgat gcctgcacct tttgttttgg gattatctga atggtactat
      961 catgaccaat ttatcactga agaccaatat attgatgcga ttaaggtagt gaatgattat
     1021 cagcttagga gatattttaa tggggatgat acaagcagag tgtcaaaagc atttccttca
     1081 tatttgaaaa gtgttaggaa gtacgctaaa gctaatggat atgaaaatat cgttgatatt
     1141 gtaatttatg ttcttgttac tagaaatcaa tcaaataata tggcacttcc atctgacaag
     1201 gcattgaaat ctaatctatt gaatgccaat gcttatgcga tgagattggc ccgttggtta
     1261 cttgaaaaaa tagagaatcg agaaaattca gcaacacttg atatgagtaa cttgtccatt
     1321 gaacacatca tgccacagac tagtacttca tattgggaag agaaggctgg tacatcgggg
     1381 gaagaataca caggattagt aaataccatt ggtaatttaa cactcgttac aaatcctgat
     1441 aattcggccg cgggaaataa agactttgag accaaaaaga aaatctttga agataccctt
     1501 catattcgta tgaataaaga tttgtacgaa ttgactgagt ggacatcatc ggatatttct
     1561 gccagaagtg aagctctaat caatgaactg attactatgt atccctattt gagatcgtcg
     1621 ggcgactatg aacacgatgg gaatagggaa attttcttag aggcgcaagg aataaaagca
     1681 actgggtatt tgaacgaaga cgagaccgtg attatacatt caggttcaga aatttattct
     1741 aagattaaag atattgcatc tgattcgcta gatgaaactc gccaagagct gttggataat
     1801 ggaatcattg aagaaacaat tgggggacta caatttgttc aagattacac tgcctcatct
     1861 gtttcgaatg cagcagctct gctattaggg ggtagccgga atggttggga ctactggaaa
     1921 tatgataacg gaataagtat taatgattcg ctaagaaata agaaatagag catagaggtg
     1981 gtgttaggtt ggataagaac aaactaagat atgcaattct taaattgcgt gatgaaaaaa
     2041 agaatccttt tattgagctt aaagggcaag ttccagaaga agatatttta gaacaaacta
     2101 aatttctttc gcgcaatggc ttactagaat ccatggtttg ggcggataac actgttcata
     2161 tctgggggag tgtttctctg gaaggggaaa aatatttagt tgaaaactca ggtcttgcta
     2221 aggcgtatgc attggcaaaa gaagttaaaa attggattcc attttggggc taagataagt
     2281 ttttaaacgg agaaggtagc aaatcataat tggtttgttg ccttttttga ttgcccaaaa
     2341 agaaaaatga caaagtatag agataaatga catagtacac gggaaaaatg acattgtatc
     2401 accgacaacg tctaaataga tgttgatata tatacgttcc gtacaaataa ttataaaaat
     2461 ttaaatgatt tgagtagaag tatcagtgaa tatctcaagg ggaatagcta tctcaagatt
     2521 cgattttcac atattattat ttgtcaaatt aagcgagaca aaactt
//
LOCUS       DARBFO010000173         2532 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000173 DARBFO010000000
VERSION     DARBFO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.178"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1016
                     /locus_tag="SCK51_002909"
     CDS             <1..1016
                     /locus_tag="SCK51_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEG1253459.1"
                     /translation="LQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFMDNELT
                     DDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTNGSLVT
                     QDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEEVDVAF
                     CPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLVSDINY
                     IKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGNIKCHS
                     LSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILDEYKHD
                     ILI"
     gene            1072..1290
                     /locus_tag="SCK51_002910"
     CDS             1072..1290
                     /locus_tag="SCK51_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253460.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1361..2176
                     /locus_tag="SCK51_002911"
     CDS             1361..2176
                     /locus_tag="SCK51_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253461.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
     gene            2240..>2532
                     /locus_tag="SCK51_002912"
     CDS             2240..>2532
                     /locus_tag="SCK51_002912"
                     /inference="COORDINATES: protein motif:HMM:NF025015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEG1253462.1"
                     /translation="MILLILSLVLLVVSNLMKNNPPTKINSFYGFRTKKSMKNEKSWH
                     LAQKLLGDFSKKKVIPVFLMGIVFFSIEIYSLAILKNENFFVSVLMGESLILIL"
BASE COUNT          958 a          284 c          444 g          846 t
ORIGIN      
        1 aacttcagat gggtgaaatt gttagaggtc ctagtcaaat atctattgat gttacatata
       61 aatgtaattt tagatgtaaa cattgttaca atgcgagtgg tggagacaat tgttttatgg
      121 ataatgagtt gactgatgat gaactgttag aacttacaaa agatatatgt aatttaaagc
      181 catataatat ttgtttctgt ggaggagaaa ctttactgag aaaaaaaata gttttatctt
      241 gtgccacttt attaaaagag gcaggtattc ctaatattgc tattgttact aatggatcat
      301 tagtaactca ggatgttgca aaagaattat tcaaagcagg tattaatcga tttcaagtaa
      361 gtttagatgg aaaaaatgct agtagccatg aagcacttag attatatcca aacagttttg
      421 aaaaggcaat taatgctatc aaattattta gagaattgtc cgaagaagtg gatgttgctt
      481 tttgtcctac tagttttaat tacaatcaat tacgagaagt atatcaacta gtaaatactc
      541 ttaaagtgaa gtctttaaga attcaaccat ttatgcttag tggtagggga atgcaaaata
      601 gggaaatagt gccaactgtt tctcaatata gaaaactagt ttctgatatt aactatatta
      661 aaagaaatga agattatacc aagatagatt ggggagatcc aattgatcat atcatccgac
      721 acagaacttt attgaaagat ttttcgaatt ttgtttctat taggtcagac ggaggattaa
      781 tggcatcacc ttacttgcca attcaagttg gaaatattaa atgtcacagt ctttctaaat
      841 attggcgagc aggactttat aaagtttttt ctttagattt agttcaagat atagctactc
      901 aagtttcttc aatacaagat atgagtaaag aattaaaaaa aggacatatt aaatggctta
      961 atgaagatat agaaattgat attctcgatg aatacaaaca tgatatttta atttaggttt
     1021 atcattggaa agtttagata aacaaagtac ttttagaaag gagggaaaaa aatgatttct
     1081 gaacctcaaa tgaataatat gtatgaaagc ccaactataa tgggagctgg tgaagctgcg
     1141 ccaagagctt gttcagcaat tatggtgtgt gtgttattag catctgcagg agtaggtatc
     1201 caagcaggag cggtgtatac ttatgctgta gttgctacag tagctgtagc tgcagccgca
     1261 gtttggaaaa cagtaggatt agttggttag gaattaatag gagactaact gtctcctatt
     1321 gattccaatg attaagctaa ggaggaattt taagaaaata atgaaataca ataatttaaa
     1381 accgaaatta cttaatgaga acatagaata tataagagaa gaaaattatg gatatttaac
     1441 catagttccc aaaaatggaa ctgaattgaa agataacgtt ttcaatccaa cagtaagata
     1501 tatattggct aactgtactg gaaaaaacag tgtaaaaaaa atagcgaatg gtttaaaaaa
     1561 gaagtttcct agtttagaat atgaaaaatt ctatcaagat gttgtaggaa ctattaaatt
     1621 tttcaaacac tataatatta ttgatatagg agattatagt atggaagaaa agaaaatata
     1681 taaaattgaa caaaatttag aattagaatt actaaatgaa tcacattatg aagaaattta
     1741 taattttatc acagatcatc ctgaaagtat gtattttcat gaagatattt atcatcaagg
     1801 aaatagtaaa gaattagaag ttgtccttag atataattta ttttcgttta cgagtgaatt
     1861 ttacttttta agacataaag agagaattgt aggaatggtt tctataattg ttaatccatt
     1921 tcaaaactta tcctacagta tagtaaatta cctcatatgt gagcctgcat atactacgac
     1981 tattttgaca acagtattag aaaatattaa cgaagtttca attttagaag taaagaagct
     2041 agtgattcat ttgctaaatt caacagaaga tgaatcacta atcccctatt taaaagattc
     2101 acattttttt aaagaagcgg tactatctaa agaatataga gagcatgatg ttttgagata
     2161 cgcttacatt tattaaataa aatttctgat agtattttaa taattcggat catatcataa
     2221 gatataggag ggaaaagaag tgattttatt gattttgtct ctcgtattac tagttgtatc
     2281 taatctaatg aaaaataatc ctcctacaaa aattaattct ttttatggct ttagaactaa
     2341 aaagtcaatg aaaaatgaaa aaagttggca tttagcacaa aaattgttgg gggatttttc
     2401 aaaaaaaaaa gttatcccag tatttttgat gggtattgtt tttttcagta tagagattta
     2461 tagtttagct attttaaaaa atgaaaattt tttcgttagt gtattaatgg gtgaaagttt
     2521 aatcctaata ct
//
LOCUS       DARBFO010000174         2526 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000174 DARBFO010000000
VERSION     DARBFO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.179"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            128..1363
                     /locus_tag="SCK51_002913"
     CDS             128..1363
                     /locus_tag="SCK51_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253463.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
     gene            1504..>2526
                     /locus_tag="SCK51_002914"
     CDS             1504..>2526
                     /locus_tag="SCK51_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253464.1"
                     /translation="MKNTIFSDEYVEIIISDHSKNTKEIAKKIEEKKVFDGCHYLESK
                     SFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFDEFICFSMGEVWLEGIYACL
                     YRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLKKRTPLYNKSYNFYCFFPNI
                     YTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKYIFFTSVYDFEGGEPVGEYE
                     LVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLSGDFNDKIFMT
                     INSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAYDIERLLKQKTMKNILKNVK
                     IAESLDD"
BASE COUNT          907 a          269 c          442 g          908 t
ORIGIN      
        1 cattaaagaa aacttgtatg gaaaaatcag tacaatacca ttatttaatg ttcttcactt
       61 aaagcgcaaa aaatatcagt ttattaaact cgtatataga acaaatatat gtagacgggg
      121 cgaaacgatg agtgtaaaaa aaatgctatt tatggtaagt acaataattg tattaacatc
      181 ccgattttgg ggaattaata ctagtttatc tatgcgatat tttattatgg caatatggga
      241 tttatatttt atgttaagct atttttctta tcgtgagaaa acatataaaa aatacgttat
      301 caagaataac ttttggttga ctataatgcc gtttgtgata ttttctatct atactctaat
      361 aatatgggga attggttcaa atgcagaatt tgaaaatttt ataaagttat gttccacgtt
      421 gctttattta attcttgcat atggttacgc atgtacagca ttttacttat ttaaggcaga
      481 aggaattgat tttatttttt ggtcaggtgt tatttcatat atgagtggtt cagtttttta
      541 tttaattgta tcttatggtt taaatggttt aatttcttat ataaaaagtc ttataacagg
      601 tgaaacaaat actgcaaatt ttgctatgga ggtacatgat ttaacttttg cgatgggggt
      661 ttttttcctt tattacgtat tttttgaaaa taaaaataca aaaaaacata agataaaaat
      721 tatcgtatct ttaattttaa ttttcatggg attgaagaga attgaactgt tagctttaag
      781 tggtgcaatt ctagtgtatt atgttttatt aaagtgggga aaaaagattc aaatccgtgc
      841 attagtgtgt actatttgtg caactgttat ttcgttagtg tttatttaca ttatagatac
      901 aggaattttg gcattgctta tgtcaaattt gggcattaca gatgacggtg cgaggctttc
      961 attttatcta tattcatcta aattttatga acttggttta ggatatgtag gaacaggatg
     1021 gacatggttt tcaaaatact tttttaatct ttatttatcg cactttaggt taaatggtgg
     1081 aagaattgca gcaagcctac acagtgatat attaactatt tatatagaag ttggatgtat
     1141 agtttttatt atatggatgt tttggttatt ttttgtgaaa ccgataagac taactaaaaa
     1201 atttggattg aaaacagggg agtgtgtatt attattaact atatatatgt ttatattata
     1261 tttgacggat aatacgctca cttatccaga cactcaaatg atttttctct tagtacctat
     1321 gatagtttcg tgggttgata atcaacaacg aatatcggaa tagatagcta aagtatattt
     1381 taattatgaa tttataatta gttgtaaaag ttaatattta acggacagga agtagggtga
     1441 aaaaatttga aaaaaatttt agtgttagca aatacatatt atcaaataat agtagcaata
     1501 caaatgaaaa atacaatttt ttccgatgag tatgttgaaa ttattatttc tgatcattct
     1561 aaaaacacta aagaaattgc gaaaaaaata gaagaaaaaa aagtgtttga tggctgtcat
     1621 tatttagaaa gcaaatcttt tgatgataat attgggtatt ttcaaaagat taaggactgt
     1681 cttcagattt cttgtggtaa atcaaatcga tatgcctatt atttgaaaaa ctgtaaaagt
     1741 gaatattttg atgaatttat ttgtttttca atgggagagg tttggttgga aggaatatat
     1801 gcttgcttat atcgaaataa taaaaatgta caggtatcaa ttttagaaga aggtatttta
     1861 agctatggag ttgacattat tccaacagca agaacaaaag ctatcgcagt actcaattac
     1921 ttaaaaaaaa ggactccact atataacaaa agctataatt tttattgttt ttttcctaat
     1981 atatatacag gaagagagaa tacaatacaa attccattag ttacagctgg gtcaagaacc
     2041 gcattgcaac tttcggatat ttttaaccct atgatagata tttataaaga aaaaaaatat
     2101 atatttttta cgtctgttta tgattttgag ggcggagaac ctgtcggaga atatgaatta
     2161 gtatgcaaaa tagcagactt agtaggaaaa gataatctaa ttgttaagac gcatcctaga
     2221 gatgtaagaa caatttataa agataatgga tttatcgagg ataaaaattc atcaatacct
     2281 tgggaggcga ttcaattgtc aggcgatttt aacgataaaa tatttatgac aattaattca
     2341 ggaagtgtta tttcaggtag tacaatgtct gcaaaacctg tacgcacgta ttatatgtat
     2401 aaattgtgta acatagaagg aaatccgtct tgcaaaaaga atgcgtatga tattgagcga
     2461 ttgcttaagc aaaaaacgat gaaaaacatt ttaaagaatg tgaaaatagc agaaagctta
     2521 gatgat
//
LOCUS       DARBFO010000175         2427 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000175 DARBFO010000000
VERSION     DARBFO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.180"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            123..2276
                     /locus_tag="SCK51_002915"
     CDS             123..2276
                     /locus_tag="SCK51_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEG1253465.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          829 a          426 c          537 g          635 t
ORIGIN      
        1 caaaaagttg attttatctt gaaattaggt aaagaatttc ttgctccgcc cattttttct
       61 tttttattaa gaagtttttg ggtacaatga agcatgaatg taaataggcg gaaggatagg
      121 aaatgatgaa agattttatg aaaaaaatat taaatacttc ttggttagaa aaaataagaa
      181 aaggtgcaca agaacctaag aacaaaacgt ttcgccggtc ccatgttcct tttcgcttaa
      241 attttctttt tttcattata tttacgttat ttgttgcgtt gattgctaga ttgggttatt
      301 tgcagatcgt gaacggcgaa gagatggagg cgcgagtgaa gtctacttct acgattacgg
      361 tccaagaaag tactcctcgc ggaatgatct acgactccac aggaaaagct ttagttacga
      421 ataaagcgaa tcaggccatc acatatacga gaggaacaca agttacggct aaagatttgc
      481 ttgaaatcgc aacgaagctg aactcgctga tcgatgttcc ggtagatgaa aatctgacag
      541 atagagataa aaaagatttt tggctggctg atcccaaaca tttgaaagaa gccacaaaaa
      601 gactttcggc aaaagaaaag caattaggga acagcgaaca atatagtgcg atggtcaata
      661 aagtcacagc tgatgagatt aatttaaacg aggagcaatt aaaaattgct accatcttta
      721 aacggatgaa tagtgcatat gaattaaaca ctgtctatat caaaaactct ggtgttactg
      781 atcaagagtt agctgttgtg gcagaacgcg cttctgaact tccaggagtt tcgactggca
      841 cagactggac acgagaatat aatgcagcaa gttcactgaa aagtattctt ggcagtgtga
      901 cgactgaaaa gcaaggactt ccagcagatg aagcagagaa atacctagca aaaggttatt
      961 ctcggaatga ccgtgtagga caaagttatt tggaaaaaca atatgaagat gtcctgcaag
     1021 gaaccaaaac ccaatatgaa gtttcccttg ataatgaagg aaatgtttcg aatcaaaaag
     1081 aaatcttctc tggggaaaaa ggttctaatt taatgttatc tatgaatgca gaattccaat
     1141 caaaagtaga agaaattttg aaaaggaatt atcaaacatt gatcaacaac ggcaaagcac
     1201 agtattcgcc tggtgcttat gcagtggcta tgaatcctca gacaggagaa gtgctagcaa
     1261 tgactggttt cagtcatgaa caaggttcca aagaaatcac cgagaatgct ttaggcacga
     1321 ttactagcgc ctttgatcca gggtctgtag tcaaggctgg tactttgaca gcaggctggg
     1381 caagtaacgc gatcagcgga aatcaagttt tgattgatga gccgattcgt ctgcaaggtg
     1441 cttcagaaaa aagctctgtc tttaatcgaa gcggacaagt tgctcttgat gcagtcaaag
     1501 catcggaatt gtcttctaat acgtatatga tcaaagtggc gttaaagatg ttaggactag
     1561 actatacacc tggaatggga ctacctagtt tagatgaaga agctaaagct tatcagcaat
     1621 tgagagactc tttcaaagag tttggtcttg gtacaacgac tgggatcgat ttgccgaatg
     1681 aatctcctgg gatctcacgt tctgttgatt atatgaagaa gttcaatgcg gataatggca
     1741 aagaatggta tgctccaggt aactttaccg atttggcatt tggacagttt gacacatata
     1801 caccaatcca attagctcaa tatgcttcaa cagtcgctaa tggcgggaaa cgagtacagc
     1861 cgcacctagt caaagcgatc tatggcaatg acgaaaatgg aaacttgggt gaagtgaaga
     1921 aagaaattgg cacaacagta gaaaatacgg tcaatatttc tgcagaaaat atggcgatcc
     1981 tccgtgaagg attccaccaa gttgtccatg gtaccgatcc ttacacaaca gccaaaccat
     2041 tagccagtgc gaaaatggac ctttccgcaa aaacagggac tgcagaaacg gttgcggaag
     2101 gtcatccaga tattgcgaca gtcaatagta atatcgtggc atacggacca acagacaatc
     2161 cggaaattgc catcagtgtt gtcttgccga acttattaga cgaacaagac cacataaact
     2221 tgacgattgc taaagaaata atggacgcgt attatgatat gttcatggca aaataaaaaa
     2281 gaggtcgtga aaaagccgct tagtttcgag tatgaagaaa aattttgtga aaatagcttc
     2341 tcacattttt gcttaaattt gacttcatac cgagaaactg gcttttgaac accgtttact
     2401 cggcaaaaaa ggttgtgaca aaaattg
//
LOCUS       DARBFO010000176         2408 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000176 DARBFO010000000
VERSION     DARBFO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.181"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(243..464)
                     /locus_tag="SCK51_002916"
     CDS             complement(243..464)
                     /locus_tag="SCK51_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253466.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="SCK51_002917"
     CDS             complement(562..807)
                     /locus_tag="SCK51_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253467.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1047..1661
                     /locus_tag="SCK51_002918"
                     /pseudo
     CDS             1047..1661
                     /locus_tag="SCK51_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2031..>2408)
                     /locus_tag="SCK51_002919"
     CDS             complement(2031..>2408)
                     /locus_tag="SCK51_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEG1253468.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          816 a          419 c          332 g          841 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
      901 ataaactatt taacttttag aattcaaaaa ccgtttgtct atttagagtt taccctaaat
      961 agacaaaaat ataacattga aaaatttaga gttctaacgt atgattaggg tgtattataa
     1021 acgttataga aaagtaggga ataaatttga aaattgctta tattagagtt tcatccatcg
     1081 atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa aaaattttta
     1141 tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa gctttagatt
     1201 ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga cgcaattatg
     1261 acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt attatcacca
     1321 gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc atcaaagact
     1381 taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg aaacgacgcc
     1441 aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt cctaagttat
     1501 acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc gttcaggatc
     1561 ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact aggcaaacaa
     1621 tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt tgaatcacgt
     1681 aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc tagctaagtt
     1741 gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc ataaatcgtc
     1801 cttatttaaa ggcatttaac attgacaact tagtataatg tatcttatgt aaactaataa
     1861 gatttgaatt ttattttata cacactcgat aatagatcaa ttttatggtc ccccgcattt
     1921 acgattgcac aagagaaaca taaagtcgac ctattagcat ttcttttcaa ttcaaacatt
     1981 aaaaaagttt atacctctgc tactaaaaaa tgaaattatc tatttaaaat ttaaaacggt
     2041 aaatctgatt catccaaatt ggaaaatgga tcagcctgta ttggagcttt gtatttctcc
     2101 ataataacag atatcttttt ttcatttttt aaaaaatacc ttttaaattt cttatagtcc
     2161 tctgaaatca aaatatagcg tgatggatac tcatcaaaag aagaatcgta ttcttccttt
     2221 aaaattcccg atttacttaa tttttctagt acatcaaata ctctatcact caagtaactc
     2281 cttaaattgt tatcactttc ccattgaact acttttctat aagttgaatt atcagttgaa
     2341 aagatagaat tttcatccat aaacgcatat attagcagca atctttcatc attgtcaaat
     2401 tctattgg
//
LOCUS       DARBFO010000177         2407 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000177 DARBFO010000000
VERSION     DARBFO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.182"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            5..1150
                     /locus_tag="SCK51_002920"
     CDS             5..1150
                     /locus_tag="SCK51_002920"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEG1253469.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="SCK51_002921"
     CDS             1321..1779
                     /locus_tag="SCK51_002921"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEG1253470.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="SCK51_002922"
     CDS             1938..>2407
                     /locus_tag="SCK51_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEG1253471.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DARBFO010000178         2398 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000178 DARBFO010000000
VERSION     DARBFO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.183"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            207..2069
                     /locus_tag="SCK51_002923"
     CDS             207..2069
                     /locus_tag="SCK51_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1253472.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="SCK51_002924"
     CDS             2069..>2398
                     /locus_tag="SCK51_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1253473.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DARBFO010000179         2381 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000179 DARBFO010000000
VERSION     DARBFO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.184"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..339)
                     /locus_tag="SCK51_002925"
     CDS             complement(<1..339)
                     /locus_tag="SCK51_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1253474.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
     gene            complement(356..760)
                     /gene="tnpA"
                     /locus_tag="SCK51_002926"
     CDS             complement(356..760)
                     /gene="tnpA"
                     /locus_tag="SCK51_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HEG1253475.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            1119..1373
                     /locus_tag="SCK51_002927"
     CDS             1119..1373
                     /locus_tag="SCK51_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG1253476.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            1374..1691
                     /locus_tag="SCK51_002928"
     CDS             1374..1691
                     /locus_tag="SCK51_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG1253477.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          794 a          374 c          434 g          779 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgccaccag
      421 ttgtcagaag acaaaagctt ttagaccaga acatttcttt ccaaagaaat tgtttgactc
      481 ttggaaagtc acgttttatc aatcttgagc tggcactttt ataggcattg atgaattttg
      541 tcaattctgt tttaggttga gctttgaaca taatatgaac gtggtcttta tcatgattcc
      601 actccactaa agtaatgtga tacgattctg aaattctttc aaaagtagtt ttggcataat
      661 cagatatttc atcatcaatc acttgtctac gatatttcgt tactaaaaca aggtggtaat
      721 aaagaagaaa tactgaatga ttatttgtat ctaattccat gttatatcaa ctcatttctt
      781 gtatagactg attgtaacat gggacaaaat aaaaaggcaa tacgccttag ttgtcggtct
      841 tcgagttacc aacggtaacc ctcataccga acggcattca tcacccacct atagaggatg
      901 ggcgacttct gcctaattct gttaaatgga tgttaacgga agtgtatggt gagtagttca
      961 ctcgtatttt gatatcgttt gtatatgttt tcagcgttct aagtggctta ttttgtccaa
     1021 taataatatg gagtggaatc ccttaagaaa caccgttggc aattgttgtt ataacatgtt
     1081 ataatttatt catcaaaaac taaaaggagt gtgataacat gggaaaagaa ctaaaggtta
     1141 gaaagatcgg caatagcgtg ggagtgatcc ttccaagctc tcttggatta aaaagcggag
     1201 acattatcca agccaaacaa gaagacaact tattcatatt agatactact caaatagcaa
     1261 aagaacacga tcgcaaattg attgaagaaa gtttccaaga ctttgagaaa ggtttaacag
     1321 tttcagaaat tgaaatggta aaagctttcg gaaaatacgg ctggagtgaa tagatgaaag
     1381 agtacacgct agtttattct aaaagttttc agaccagcct tgaaacgata atagaagaat
     1441 gggaaaatga gcttgctctt tctccagcca gcataaaaag atttgtaggt gcgatccaga
     1501 aggctttaga actaaccaaa aattttccaa ctatgcatga ggaagtttcc gaaatctatg
     1561 gaatggatat cccaacatat cggatcgtga tcggcaaaag ctatgcctta ttttatcgga
     1621 tcgaccatga aaaaaaagag atacttgtag gacgtatttt ccaatctaaa caaatgaaac
     1681 taggctttta acaataatta ggaaaaagtc gctcatcctc tacaggtggg tgattaatgc
     1741 cgctcgatat ggttgttaac cgtcggttac tcgaagattg atatataaga cttagtacct
     1801 ttttattttg ctccatgtta tggccgaagg tctaaagata caatcattag tctagagaaa
     1861 aattaagaac cttaggaact acggggatcg cttggtgaat aagagacact gctattagtg
     1921 aaaaaataag atatcaagta cgctctattc ccaaaaattt cgtgacttta ctcatgagag
     1981 gttcaagtag tatattttta atcaaaattg acgtaacttt taataaatac tacctaaata
     2041 aggtagtatt tatttttgtg ttttaaaaga ggaagtccaa tatatctatg ggaaacttct
     2101 taaatattat gttggtacat gaaaatatac ttccctgcta gactatttac aacattttac
     2161 tttgtacgta ttcttgtttt aaaaggaatg ggataaaata taggtacaat aactatttga
     2221 tgctaaatat aattaatgga ggagagatag tgaacatact taatgaaaaa agatatgtat
     2281 ggtttgctgg taaaaaatat attaacccgt ggtgctagtt aattttaata tcctaaagaa
     2341 tatctcaaag ttccttcaaa acgtcgttgt gctcgactta a
//
LOCUS       DARBFO010000180         2373 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000180 DARBFO010000000
VERSION     DARBFO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.185"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            149..2314
                     /gene="acm"
                     /locus_tag="SCK51_002929"
     CDS             149..2314
                     /gene="acm"
                     /locus_tag="SCK51_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEG1253478.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
BASE COUNT          935 a          355 c          456 g          627 t
ORIGIN      
        1 catatttatt ttttataaat atttgtaaaa taactatcaa ttttttagtt aaaagattgg
       61 aaatgagtgg gggaggaatt gaagaaagga tttttgagag atgatatagt aggttttatt
      121 tttagttaaa ataggggaga tagaaaaaat gaaaaagtgt agaaatatta ttttatcaat
      181 gttatttatt attactaata taacatcact gattcctgtt catgtctatg cggatgcagg
      241 cagagatatc agcagtaatg tcacttcgtt gaccgtggac ccaacgaata ttacagatgg
      301 aggaaacata aaagtaaaat tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga
      361 ttatctttgg atcaattggc caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga
      421 aatcccatta atgattgaaa ataaaaatgt tggaacttta acggtcagaa aagattctgc
      481 gcaggttgtg tttaatgaaa atattaaaaa cctagactca gtagaaggtt gggggcaatt
      541 tgagatacag gccagaaacg taaccgatac cggtgaagaa aatacgggac cattcaccgt
      601 gacgagcggt gataaaacag ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc
      661 tagcgttttt tattataaaa caggagacat gctacctgaa gatactaagc acattcgatg
      721 gtttttgaat attaacaata acggcactta tgttgaacaa ccagttaaaa tcagtgatga
      781 aatccaaagt ggacaaagat tagatccaag tacgtttgaa attaatcaaa tacacttagg
      841 tgaacagaag gtttatagag gagaagaggg aatccaacaa tttttacaag atttccccag
      901 cgctacattt aatttttcag taactgataa ttacattgaa ataactatac caaaaaactt
      961 tgtcaatctt aggaaaataa tggtgagtta taagacgatc atagaaaacc cagaacaaat
     1021 aaattttgag aatcattcgg aagcttggtt taaagaattt aataaacctg ctgttgatgg
     1081 agaaagtttc aatcatacag ttaaaaatat ttcagcttct ggtggagtca acggtacagt
     1141 tagaggcgaa ttgaagattt tcaaatatat caatgacaca gagattggta tcccaaatgt
     1201 gactttcgaa ttaagacgag cagatgaaca gcctattcaa ggacagtcta gtatcctgtt
     1261 gacttcgaat gaacagggag aagctagtat caaaggcctg caagtaggag actatgttgt
     1321 aaaagaaaaa gaagcaccca attggattga ttttgatcca ctaagttcaa atgaactcaa
     1381 attctccata aacgaaaatg atacagaggg agtcagttta cctatttata acaagaaaaa
     1441 ggttacgaac atcacagcaa ctaaaatttg gaacggtgga accactccaa gaccgagtat
     1501 ttattttaaa ttattcagag caagtacaaa caattgggaa ccagttcctg atgcagaaac
     1561 aaaacgatta gacaatggaa taacttcagt gacttgggaa gatatccaac agtatgatga
     1621 tagtggaaac gaatatacgt ttaaagtgca agaagtagat caaaacggaa atgattatgt
     1681 cccttcaggt tatcaaaaaa ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt
     1741 catttcagtc tcaggacaaa aaacatggga agacaatgag aaccaagacg gcaaacgacc
     1801 gacgacgatc acagtaaatc tattagccga cggtcaaccg atccaacaca aagaagtaag
     1861 tgaaaaagat gattggcgct atcgtttcac aaatctgcca aaatacagag acgggcaaga
     1921 aatcatctat actgtaacgg aagacaacgt gcccgaatac agtacgacga ttgacgggta
     1981 taacatcaaa aacagttata caccggaaaa aacaaatata tctataccag gtagccatat
     2041 aacaccgtgg aaaaattttc cagagaagac agagagagag aaaattagaa atctattctc
     2101 tcaaaaattg caaatatttg ctcatacagg agaatccagc gaacaaagaa gcaattatat
     2161 tagtaagaga gctgtaccta aacagataac aaataaatca aaaagcattc ttcctaaaac
     2221 aagtagtaaa aaatctagtt ttgctgtaat cattggttta ttgatagtaa ctatctgtgc
     2281 tgggatattc ttacaaaaat ataaaaaagt ttagttagaa tataatattt tttgttaaat
     2341 atgactgaaa tcaaaagaat aaaattccga ttt
//
LOCUS       DARBFO010000181         2306 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000181 DARBFO010000000
VERSION     DARBFO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.186"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..492)
                     /locus_tag="SCK51_002930"
     CDS             complement(<1..492)
                     /locus_tag="SCK51_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HEG1253479.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLE"
     gene            1054..>2306
                     /locus_tag="SCK51_002931"
     rRNA            1054..>2306
                     /locus_tag="SCK51_002931"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          666 a          467 c          561 g          612 t
ORIGIN      
        1 ttcaaggtga ccaaactcag aattgaaatc aggataatta tcaggtcgat catggattga
       61 gcgctgatac tgttgttttc cccgtttttc ctgatggcca ttgggttttc ttttaccttt
      121 catcggtagt gtgtcaatat caaatattcc tttagaaaat aaacgataaa gtgttctcat
      181 accgcatgaa acaggccttt cttttcgccc gataatgacg tcaggcgtcc aaccaagagt
      241 gactttctct ttaatgtaat ctacttcatg agcaggtaat tgaatgactt ttctaccaca
      301 ttttttctta ttttctttat actggtgcca ataatcaaga gcagtcttgc cttgcttgaa
      361 cttattgact acattataaa tagtttgtat agcacgtccc attcggttag cgatttcaac
      421 cggcttatta tgttgaagat aatatgattc tatcattgtc agttcgtcta tggtaagatg
      481 tttgtaggtc atttatggtt acactccttt gttttctttc gtcggaaata caatttgagt
      541 gtaccataaa tgccttttta tttttctaac ttaattttac aattcgcgat atattaaaca
      601 acgttgtcgc gcttcattga aacattttat gaaaattcca aaaaagagtt gacacatgtt
      661 tttagacgtg ataagataac taagtcgtta aaaagaacca cttgcgaagc ttgattctat
      721 cgaaaaaaaa cttcaaaaaa gttcttgaca aataacaaac gataagttat gataaatgag
      781 ttgctgaaag atttcgaaaa acgaaatcga aaaaaacttt taaaaaagag ttgacaatcg
      841 cttgtctctc tgatatgata taaaagttgc tacggcagcc aaaaacgaat cgggaaacac
      901 tcccgatgaa gtagaccttt gaaaactgaa caaagtaaga caaaccaaat gtgtagggcg
      961 tcttgattca attcaagaca ataaacattt ttaacaagca agcaatatgc tagcaaacaa
     1021 attgagctta acaatcgcaa gattgttcga actttttatg agagtttgat cctggctcag
     1081 gacgaacgct ggcggcgtgc ctaatacatg caagtcgaac gcttcttttt ccaccggagc
     1141 ttgctccacc ggaaaaagag gagtggcgaa cgggtgagta acacgtgggt aacctgccca
     1201 tcagaagggg ataacacttg gaaacaggtg ctaataccgt ataacaatcg aaaccgcatg
     1261 gttttgattt gaaaggcgct ttcgggtgtc actgatggat ggacccgcgg tgcattagct
     1321 agttggtgag gtaacggctc accaaggcca cgatgcatag ccgacctgag agggtgatcg
     1381 gccacattgg gactgagaca cggcccaaac tcctacggga ggcagcagta gggaatcttc
     1441 ggcaatggac gaaagtctga ccgagcaacg ccgcgtgagt gaagaaggtt ttcggatcgt
     1501 aaaactctgt tgttagagaa gaacaaggat gagagtaact gttcatccct tgacggtatc
     1561 taaccagaaa gccacggcta actacgtgcc agcagccgcg gtaatacgta ggtggcaagc
     1621 gttgtccgga tttattgggc gtaaagcgag cgcaggcggt ttcttaagtc tgatgtgaaa
     1681 gcccccggct caaccgggga gggtcattgg aaactgggag acttgagtgc agaagaggag
     1741 agtggaattc catgtgtagc ggtgaaatgc gtagatatat ggaggaacac cagtggcgaa
     1801 ggcggctctc tggtctgtaa ctgacgctga ggctcgaaag cgtggggagc aaacaggatt
     1861 agataccctg gtagtccacg ccgtaaacga tgagtgctaa gtgttggagg gtttccgccc
     1921 ttcagtgctg cagctaacgc attaagcact ccgcctgggg agtacgaccg caaggttgaa
     1981 actcaaagga attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgaagca
     2041 acgcgaagaa ccttaccagg tcttgacatc ctttgaccac tctagagata gagcttcccc
     2101 ttcgggggca aagtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg
     2161 gttaagtccc gcaacgagcg caacccttat tgttagttgc catcattcag ttgggcactc
     2221 tagcaagact gccggtgaca aaccggagga aggtggggat gacgtcaaat catcatgccc
     2281 cttatgacct gggctacaca cgtgct
//
LOCUS       DARBFO010000182         2233 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000182 DARBFO010000000
VERSION     DARBFO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.187"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..544
                     /locus_tag="SCK51_002932"
     CDS             <1..544
                     /locus_tag="SCK51_002932"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1253480.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="SCK51_002933"
     CDS             complement(900..1472)
                     /locus_tag="SCK51_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1253481.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="SCK51_002934"
     CDS             complement(1488..2093)
                     /locus_tag="SCK51_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HEG1253482.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          701 a          454 c          338 g          740 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt cta
//
LOCUS       DARBFO010000183         2217 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000183 DARBFO010000000
VERSION     DARBFO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.188"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1293
                     /locus_tag="SCK51_002935"
     CDS             <1..1293
                     /locus_tag="SCK51_002935"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEG1253483.1"
                     /translation="ADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGT
                     DEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVS
                     DMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLA
                     KMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSR
                     FNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGS
                     IYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQK
                     ILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIR
                     QEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1283..>2217
                     /locus_tag="SCK51_002936"
     CDS             1283..>2217
                     /locus_tag="SCK51_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG1253484.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLI"
BASE COUNT          765 a          381 c          467 g          604 t
ORIGIN      
        1 gcggatcaag caaaacaagc aacgttccaa ttgaacgatc taaacaaagc ctttgtgcaa
       61 ttggcatcta cctacaatca atttaatggg ttatttgatg atgtggatct ccaatcaaaa
      121 aaactaggaa cagatgagca acaacgaaat gtgacgatta ccgaagtcat caagaaattg
      181 aatgatgtag atgttttaga acacgatggg gacgtgatcg gggatgcgta tgagtttctg
      241 atcagtcaat ttgcttcaga agcagggaag aaagccggag aattttacac acctcatatg
      301 gtttctgata tgatggcgca aatcgtaacg ctcgatcaaa aggaacgacg attctttagt
      361 gtttttgatc caacgatggg ttcaggttca ttgatgttaa acgtacgtaa ctacttaaca
      421 cacccagata acgttaaata tcatgggcaa gaactaaata caacgacata caatctagca
      481 aaaatgaact tgattttaca tggtgtcgac gccgaagaaa tgaatctccg caatggggat
      541 acgttgaata aggactggcc aacagatgaa ccttacacat ttgatgcagt agtaatgaac
      601 ccaccttatt cggctaattg gtcagcagat acgactttct tagatgattc tcggtttaat
      661 cgttatggga aattagcgcc aaaatccaaa gcagattttg cgtttcttct tcatgggttc
      721 tatcatttga aggaaacagg taccatggcc atcgtcttac cacacggagt tttattccgt
      781 ggcgctgcag aaggggtcat ccgtcaaaaa ttactagaag atggcagtat ttatgctgtg
      841 atcggtatgc ctgcgaactt gttctttggg acatcgattc caacgacggt gattgtgttg
      901 aagaagaatc gtcaaactcg agatgtatta ttcattgatg ctagtcgtga atttgttaaa
      961 gggaaaaatc aaaataaact atcggaagaa aacattcaaa aaatcctcga aacctacgct
     1021 gaaagaaaag atgtcgagaa atacgctcat ttggcaacct ttgatgaaat caaagaaaat
     1081 gactataact taaatatccc acgctatgta gatactttcg aagaagaaga accaattgat
     1141 atggttcatg tcggaaatga catcaagaag atacgacaag aacagcaagt gctagaaaaa
     1201 aaacttttag aagctatctc ttccctgcaa acaacacctg aaaatgaagc gtggttacaa
     1261 ggtgcgctgg aggtgttcaa acatgagcaa tgatacacaa ccagaaatcc gtttcccagg
     1321 atttacagat gattgggaac agaggaagtt gggggacttc gttgtagatt atgttgaaaa
     1381 gacaagtgta caaaatcaat ttcctatgct aacttcatct caacaaaaag ggatagtttt
     1441 gcaagaagac tattttgcaa atcggcaagt aactacagaa aataatattg gttactttgt
     1501 actacctaga ggatatttca cgttccgtag tcgaagtgat aatgatgtgt ttgtgtttaa
     1561 ccgtaatgac attattgata gagggattat aagctatttt tatcctgtat ttactttaaa
     1621 atctgctgat tccgacttct tcttaagacg tattaacaat ggaatccaac gacaattatc
     1681 tattcaagca gagggaacag gtcaacatgt tctgtctcta aaaaagttta aaaatatagt
     1741 agcaatgttt cctagtgagg aagaacaaca aaaaatcggc accttcttca aacaacttga
     1801 cgacacgatc gctcttcatc agcgtaagtt agatttgctg aaggaaacga aaaaaggatt
     1861 cttacaaaaa atgttccctg aaaacggagc aaaagtgcca gaaatccggt ttcctggttt
     1921 tacggaagat tgggaagagc gtaagttggg agaattaggt aaaactcaat cgggaattgg
     1981 atttcctgat gccgagcaag gtggttctga aggtactcca ttttttaaag tttccgacat
     2041 gaataacatt ggtaatgagt atgaaatgag aaatgcaaat cattatgtat ctaatgagca
     2101 aattgagcgt aaaaaatgga agccaataaa agatgtgcca gcgattatat ttgctaaagt
     2161 tggcgcagca ataatgctta atagaaaacg tttggtcaca agccctttct taataga
//
LOCUS       DARBFO010000184         2157 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000184 DARBFO010000000
VERSION     DARBFO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.189"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..914)
                     /locus_tag="SCK51_002937"
     CDS             complement(<1..914)
                     /locus_tag="SCK51_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEG1253485.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="SCK51_002938"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="SCK51_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEG1253486.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..>2157
                     /locus_tag="SCK51_002939"
     CDS             2006..>2157
                     /locus_tag="SCK51_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253487.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEA"
BASE COUNT          731 a          381 c          417 g          628 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaa
//
LOCUS       DARBFO010000185         2058 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000185 DARBFO010000000
VERSION     DARBFO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.190"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..337)
                     /locus_tag="SCK51_002940"
     CDS             complement(<1..337)
                     /locus_tag="SCK51_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEG1253488.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="SCK51_002941"
     CDS             complement(404..559)
                     /locus_tag="SCK51_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1253489.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1176..1397
                     /locus_tag="SCK51_002942"
     CDS             1176..1397
                     /locus_tag="SCK51_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253490.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1398..1655
                     /locus_tag="SCK51_002943"
     CDS             1398..1655
                     /locus_tag="SCK51_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253491.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1791..>2058)
                     /locus_tag="SCK51_002944"
     CDS             complement(1791..>2058)
                     /locus_tag="SCK51_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEG1253492.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          678 a          462 c          314 g          604 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcca cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aacgctgaaa cccttgggag agtaaggcta
      721 aacagctatc gaccaatgac caatgaccaa taaggggggt gtcgtagtcc ccccctcctc
      781 cgcccaattc ataaacacca aaaccaaaaa acaaacctca gggctccgcc ccgaaccccg
      841 acctccttct accgaaatat atgcaggcgc cacgcttact atttcgttca gctatcggct
      901 tttgcaatct aacacgaccg tttcgctacc agtcgcctta caggctccat tctcgcttct
      961 cttacgtgtt ttaacgattg cgccgataac taaacgaaat agacgctaaa tcgcttaaaa
     1021 cgcaaaatag acccttttta gagcattgat tttttctact agtcatttcg ataatataaa
     1081 attgaccaca acacagaaaa cttatattat aagcttatgt cgtaagtccg acgtactcac
     1141 gtaatataga cggacgttaa atggaaggag cataaatgag agtgataaaa tcagaaaaat
     1201 tcactttggc cgaaattatg acgaccagcg aacttgcaca agaacttgat tacaaccaag
     1261 cttacgtttt gcgcttagcg aaagaacatc tgactgaagg aaaagaatat cgctctgccg
     1321 gtagaagaaa ctatcttttc agcccagaag cccttaaaaa actagaaaaa gtcataacca
     1381 ccaaagaagg tgagtaaatg cctaaagaaa caactatcaa cgttgaacaa ctagaaaaat
     1441 tgctagaatc atattccaat gattttatcg aattgaccgg cggaaaagtc gaagaaacca
     1501 ccgacctctt tgacctactt ctcacaaata ttctggaatt aaactctgaa gaacaaaaag
     1561 agcataaggg aaaactgata gcaccttcct acaggaaggt ggctaagtcg gctgtgccaa
     1621 ctggttttct tacaaaacaa ccttatattt tttgatatca caaaaaaatc cattttggac
     1681 ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt agttttggtc
     1741 atagtgacta agactttcac gcacaaaaaa agagctgaaa ttaacagctc ttatccttta
     1801 tttatttttg aatttacact tccaccatga cgattcgatt ttatatattg cccgataata
     1861 taagctgaat tatcacaaag aagcggaaac caatcatcaa atctttctga cattgcatag
     1921 tcaattaaat ctttgatttc agtgatattc ttagaatcaa caaacgacat catctctcta
     1981 atcaactcat atcgttctgc actcgttact gacaaataac tagcaatttc tgcaccacca
     2041 tgagcgataa tatcactt
//
LOCUS       DARBFO010000186         2028 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000186 DARBFO010000000
VERSION     DARBFO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.191"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1008
                     /locus_tag="SCK51_002945"
     CDS             <1..1008
                     /locus_tag="SCK51_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253493.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="SCK51_002946"
     CDS             1025..1174
                     /locus_tag="SCK51_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253494.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="SCK51_002947"
     CDS             1171..1617
                     /locus_tag="SCK51_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253495.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="SCK51_002948"
     CDS             1619..1756
                     /locus_tag="SCK51_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEG1253496.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2028
                     /locus_tag="SCK51_002949"
     CDS             1794..>2028
                     /locus_tag="SCK51_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253497.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHA"
BASE COUNT          734 a          352 c          421 g          521 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcag
//
LOCUS       DARBFO010000187         2003 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000187 DARBFO010000000
VERSION     DARBFO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.192"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..378
                     /locus_tag="SCK51_002950"
     CDS             <1..378
                     /locus_tag="SCK51_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEG1253498.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="SCK51_002951"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="SCK51_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEG1253499.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="SCK51_002952"
     CDS             1859..>2003
                     /locus_tag="SCK51_002952"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253500.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DARBFO010000188         1992 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000188 DARBFO010000000
VERSION     DARBFO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.193"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="SCK51_002953"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="SCK51_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEG1253501.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="SCK51_002954"
     CDS             852..1040
                     /locus_tag="SCK51_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEG1253502.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="SCK51_002955"
     CDS             1053..1610
                     /locus_tag="SCK51_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1253503.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="SCK51_002956"
     CDS             1642..1884
                     /locus_tag="SCK51_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1253504.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          554 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc ct
//
LOCUS       DARBFO010000189         1903 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000189 DARBFO010000000
VERSION     DARBFO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.195"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..111
                     /locus_tag="SCK51_002957"
     CDS             <1..111
                     /locus_tag="SCK51_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEG1253505.1"
                     /translation="MYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            112..864
                     /locus_tag="SCK51_002958"
     CDS             112..864
                     /locus_tag="SCK51_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEG1253506.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            869..1816
                     /locus_tag="SCK51_002959"
     CDS             869..1816
                     /locus_tag="SCK51_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEG1253507.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          661 a          258 c          381 g          603 t
ORIGIN      
        1 atgtatgaga aggagttgaa atatttcttt gaaaacgtag taacaggcaa agaaaactgg
       61 aatgatttat atcatgcaaa cgaagtattg aaaatagctg aggagaaata aatggaaatt
      121 ttatttacta tttgtggtag agctgggtct aaggggttta aaaataaaaa tttgaaatat
      181 ttggcaggaa agcccctagt ccatttcgct ttagcaatga tagatatcta tcaacaaaaa
      241 catcctgaac ataattgcac gacttgtttg aatacggaca gtaatgagtt agtagaattg
      301 acgactaagt taaatccgtc tgtgaaaatt gtagaacgta aacctgaatt agctacagat
      361 actgtaggga aaattgaagt gatacgtgat tcattcttac aactagaaag agaagataat
      421 atatttgatt taattataga tttagatttg acttcacctt tacgtgaagt ttctaatctt
      481 gaagaattaa tagatgaaca tctaaaaaat ttagataaag acgtctactt ttcagttgtt
      541 tctgcaagac gtaatcccta ttttaatatg gtaaaaagaa ttgaaaatga agttactata
      601 gttaataaat caagatacac atctaggcaa cagacacctg aagtttttga aatgaatgcc
      661 tcaatgtatt cttttaaacc tgaatttcta aaaaaaagta attatatttt tgatgggcaa
      721 tgtgggataa taaaaatgag agattatcta attttagata ttgatagtga agaagatttt
      781 aaatggttag aatttttgtt tcctaaattt ttagaagatt cttcggagct tcaagatatc
      841 tataattatt tatatcggag gtaaaaggat gagctgttat cttatcaccg gtggggctgg
      901 cttcattggt tctaccttag ccaatcattt aggagaagaa catgtggtaa tcgtcgtcga
      961 tgacttatca atgggaaaag tagaaaatct tgatatgacg aaaaatatca cttttattga
     1021 gggagatgtc gctgagaaat ccttgatgga agagatcatg aaagctaata aatttgatta
     1081 tatctttcat ttagcagcag ttgctagtgt ggcagattca gttgcccgac cagtagaaac
     1141 acatcgtgtg aattttgaaa gtgtattaat gattttagaa ttgatccgca agtatcaacc
     1201 tgatttaaaa cggatcgtgt ttagttcttc agcggctgtt tatggggatg aaccgacttt
     1261 accaaaaaaa gaagagtccg tgattcgccc attgacgcct tatgcggttg ataaattcgc
     1321 tgcagaacaa tatgtgctag attattgtca tttatatgat gtacccggta gcgcagttcg
     1381 ttttttcaat gtctatggtc caaatcaaaa tccgaattct ccatacagtg gtgtgatttc
     1441 tattctcgta gatcgctata agaaacaaat cgtaggagaa gcaacttcat ttacattatt
     1501 tggtgatgga agccagtcta gagactttgt ttatatagat gatgtaatcc aagcattact
     1561 tttagttgcg aatgaggaag ctgcactggg gaaacaattc aatgttggaa cgggtaaagc
     1621 aaccacactt ttggaattga ttcatgcaat caatgaaatt ctcggtgtag aattgacact
     1681 tgaatacaaa gaagagcgtt cgggtgatat ccatgactct ctagcagata ttactaagat
     1741 ccagtcaatt ggctatcaac cgaaatatgg tattttaagt ggaatgacta attatttgaa
     1801 aactgaaatc aattaaaaca tactaagata agattagagg gtaaattcag gctctttgtc
     1861 aataaggact gatgagtttt gtaaaatcaa atctgggata gaa
//
LOCUS       DARBFO010000190         1848 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000190 DARBFO010000000
VERSION     DARBFO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.196"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            286..693
                     /locus_tag="SCK51_002960"
     CDS             286..693
                     /locus_tag="SCK51_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253508.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            707..1108
                     /locus_tag="SCK51_002961"
     CDS             707..1108
                     /locus_tag="SCK51_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253509.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1110..1481
                     /locus_tag="SCK51_002962"
     CDS             1110..1481
                     /locus_tag="SCK51_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253510.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1517..1816)
                     /locus_tag="SCK51_002963"
     CDS             complement(1517..1816)
                     /locus_tag="SCK51_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253511.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          691 a          247 c          308 g          602 t
ORIGIN      
        1 gaggggaagt acatatagtt gttgatatag ttgctaatat tgttagctaa tttgttagta
       61 aaactgtata cgaatttaga tagtaaaaaa acagtttgtt ccttatatag aggcactttt
      121 actactgatt aaatgtcaga tatgcttaca aatatactta taaatatgct tacaaaatta
      181 gctaatattt ccgttgacaa agctttgtta ctattgttaa actaaattga ctagcaaaag
      241 tgaaacataa atttattaaa taaatgtaaa aattaggaga aatcaatgtc ttatagagta
      301 caattaataa tttcagaaga tgtagaaagt cagcaatttg gcactaatgt tattaataaa
      361 gtaattaatc cagctctttc tattaatgcg cctttaattc ctactgcttt atcattttct
      421 gtaacagctg tggtgtctga gatagaggat acagaaaaga taaaaatagt agaaattgaa
      481 gttttaaaca agaatgaaaa acaaattttt tctacgggtg aagtatcggt aagcttgcct
      541 cctcaagtta acgatataaa ctttaatata aacgccagaa atgtcttggt tgaagaagca
      601 ggagagcatt atgctgttgt taaatttaat ggaactgaga ttggaaggca aatctttgat
      661 atcaaggtca acaaaccagt ggaaaaaaat taaggagacg atacggatgc ttgatatagt
      721 accatcaaat acacatcgga gtggtagctt aataaatttt gctgcgattt ttgcttgcgc
      781 atcggtaatt ctagcatctc cagttgttgt ggatgcaagt tctactccga aaactaaaaa
      841 tgataatcaa cttgtaatta ctacaaaaaa tgagattaac acaatttcaa gtaatgatgg
      901 caaaattttt gatgtgataa atacggtttt aaaaagaaaa gctgaaatca atataaaata
      961 tgatgaagat ttaaacttgt atttctttgt tatcaaaaca acatctgaat tgttttcctc
     1021 agattacgat gttttggata ctctcgataa tgtattatct gactataaat atatggggaa
     1081 atctgtagta gcaactttgg gggaataaaa tgtttgactt taatgaatat ttatctattt
     1141 ccaaacaaat tgaatcagat gataaatata attctaaaga atcttataga agaactgctg
     1201 tttcaagagc atactacagt gcttttaaaa aatctgatga gtatttgaaa gaaaattatg
     1261 atattattta taatggaagt agcggaaaag gaagccacca aaccgtttgg aatttgtttt
     1321 caacagttaa ggaattgaat accttaggaa tacataatag tgggtataga atgttagaga
     1381 aaagaaaatg tgctgactat attcctaatg aaaccattac gaaaacagat atggcgctga
     1441 tgaatcgaga agcagaaaaa ataataaaca aactcaccta atgaatttca agcgagtttg
     1501 tttgttattt ttttatttaa tctttgtatc tttctggatc aacgaaagta tactttatat
     1561 agtcataacg ccgatgatcg ctacgtgcgt ccggcacatc agtcacgata tcaaacaaaa
     1621 aatatacgtc tttcttcatt ctagttttcg cagcagggat tttaaagtag ttcttattag
     1681 aatagtagag attaattaat aagctatctt cgattgctaa aaagaaaact tctgaatccc
     1741 acaccttata aaaatctttg acaaatctat tcgaaggatc aaatttaaac cataattgcg
     1801 tctcattaaa aagcataacc attactccga tctattttta aacttagt
//
LOCUS       DARBFO010000191         1847 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000191 DARBFO010000000
VERSION     DARBFO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.197"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            565..>1847
                     /locus_tag="SCK51_002964"
     CDS             565..>1847
                     /locus_tag="SCK51_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1253512.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTK"
BASE COUNT          605 a          353 c          344 g          545 t
ORIGIN      
        1 tttcagcttt tttattcttg gaataacgag gtattgtttt cgataaaaaa agatggtgca
       61 gacaaattat gataattaag catactatca gataactcaa aatgaagcct tctttcaaaa
      121 aattattcaa aaccatatat tgtatttttt atttgataat aacaatggga ttattaagaa
      181 gtggcactaa ataaaagact agtgtgtatt tagccgattt aattataaac aagtaaatgg
      241 ataaagtata tgtaatgata aaagaaaaat ggattttcta atttttaagc taaatgttag
      301 atcacacatg tatatgctgg atagtcttat aaaaaatcat gtaatacagc ttggtacatg
      361 atttttattt gtagctgttt tttacataga cacaaaaaag aaaccacgct tgtagacgtg
      421 gtttcttttt taggatctat aatatctagt gttggtgacg ccttctgttg ccaatacttt
      481 ttcttttttc taataaacgc aaacagacac cttgaaatcc tttagaataa agtcgacgaa
      541 aaccattcaa aggagtgttt caagatgtcc tattcagagt ttaccaaaga gttactagat
      601 attctagacc taaatcttac ctttcatgaa gatgcctttc gcaaagagcg cattaatgac
      661 gaaacatgtt ttgtttttga tggcacattg acctatcaac cagaggaatg cttccattgc
      721 cactatcaaa ataagcaaac gatcattaaa tggggctgga aaaaagtatc catcttattg
      781 aatgatgtca gtaactacaa gaccattgtg cggattaaca aacagcgctt caaatgtaaa
      841 cactgtggaa agactttttt agcagaagat tcagttagcg atcgtcgctg ctccattgcc
      901 cgcagagtca aacaagccat tcttgagcta ttaagtgagc ctatttctat gtctttgatt
      961 gcacgtatga agcacatttc tccgaccact gtcattcgaa tacttcgcag ccttcggcct
     1021 aaaaccgttt ctttgaacca gccgttacca gaagtggtgt gtttcgatga attcaaatcg
     1081 gtgaaaaacg tctcgggagc catgagcttt gtcatgatgg acggcaagac ccatcaactg
     1141 attgatattg tggaaaatcg gcaactcaat cctttacgcg actatttctt gcgctaccct
     1201 agaaaagtcc gtgaacaagt acgtctagtg gtttcagatt tctatacacc ctatcgcaca
     1261 ttggtgaaag agtgttttcc gaatgcccga atcgtcgccg atcgctttca tattagccaa
     1321 cacatcggtc gggcctttac caaccaccga attcaagtaa tgaagacctt taaaaaaggg
     1381 gatcgtcggt cgaagcactt aaagaaatac tggagactac ttcaaaagaa tgcgtgggaa
     1441 ttgaatggtc agcatcgcta ctggcgtccg tcatttcgag atcatctgac agaagcggag
     1501 atagtcgatc ggctattata ttatgatgac tccttaaagc gaggctacga agtctaccag
     1561 gtttttcttt ccgctatcag aagacaagat gttcctgaat tcgtcgcgct cctaaaagaa
     1621 gactacaagg agttgccaga gcactatcaa ccagtgttca cgaccttcaa gaaatatcga
     1681 acagaaatca aacgagcgtt gcgagttccc tattccaacg gccctataga atgtttgaac
     1741 aaccatatca aagtactaaa acgaattgcc tatggcttta gaaactttca aaactaccga
     1801 gaacgaatct ttttatatcg aggaaaatat ttcaagaaaa ctaagaa
//
LOCUS       DARBFO010000192         1843 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000192 DARBFO010000000
VERSION     DARBFO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.198"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1085
                     /locus_tag="SCK51_002965"
     CDS             <1..1085
                     /locus_tag="SCK51_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEG1253513.1"
                     /translation="GTILGWQFYNNEHSQIVAHAQSAQKKLILTTNEQQLVEKIDQLL
                     RKNDYIGSIYVKKNNRIIMEKGYGYANKLTQQPNDPSLYYQIGSVQKALTTLLILKQV
                     ERGHLSLDTKLAQFYPQIAGSQQITIRDLLYMRSGLHRTASPTIPMTDEEVVQFAIQH
                     LKFTDYQTSHYDSLNFTILTGILIKLTNQPYEKLLTDDIIEPLGLQHTKFYDQVKNSS
                     NHALSYQMSKEDDYFKPLTESETEIHNELGTGNISMSVYDLNTLFTQVLSGKLVSKDL
                     LFSLWRENANGHPYSGGVYSGDNYILSQGNIDSFDTSAIFSKDSQDAIIMESNIKADK
                     TIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(1352..>1843)
                     /locus_tag="SCK51_002966"
     CDS             complement(1352..>1843)
                     /locus_tag="SCK51_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253514.1"
                     /translation="SILNYSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYK
                     KDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSK
                     NATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRT
                     SHE"
BASE COUNT          647 a          320 c          280 g          596 t
ORIGIN      
        1 caggaactat tcttggatgg caattttaca ataatgaaca ctctcagata gttgcccatg
       61 ctcaatcagc tcaaaaaaag ttgatactga ctaccaatga acaacagctc gttgaaaaaa
      121 tcgatcaact attaagaaaa aatgattata ttggctcaat ttacgtaaag aaaaataatc
      181 gcatcatcat ggaaaaaggc tatggctatg cgaataaact cacgcaacaa ccaaatgatc
      241 cttctcttta ttatcagatt ggttcggttc aaaaagcatt gactactctt ttgattttaa
      301 aacaagtcga gcgtgggcac ctttctttag atactaagct ggctcaattc tatccacaaa
      361 ttgctggcag ccagcaaatt actatccgtg acttgctgta tatgcgctca ggcttgcacc
      421 gaactgcttc tccaaccata ccgatgactg atgaagaagt cgttcaattt gcgattcagc
      481 acttaaaatt tacagattat cagacctctc attatgattc attgaacttt accatattga
      541 ctggaatttt gatcaaatta acaaatcaac cgtatgaaaa attattgacc gatgatatca
      601 tcgaaccttt aggattacag catacaaaat tttatgacca agtcaaaaat tcatccaatc
      661 atgcactatc ctatcaaatg tctaaagagg atgattactt taaaccactg acagaatctg
      721 aaacagagat ccataacgaa ttagggactg gtaatatcag tatgtccgtc tatgatttaa
      781 atacgctgtt tacacaagtt ttatctggaa aactagtttc caaagactta ctcttctcat
      841 tatggcgtga aaatgcgaat ggacatccat atagtggtgg ggtatatagc ggagataatt
      901 atatcctgtc gcaaggaaat atcgactctt ttgatacatc tgctatattt agcaaagatt
      961 cacaggatgc tatcatcatg gaaagcaata taaaagctga caaaactatc aaactgccta
     1021 ctgctgatct aagaaaccaa atctatgacc tgatggaagg aactactaag ttggcaaata
     1081 gctgatatga tgaaaagata gcaaatacta cttctttgaa tagcatttgc ttaaaaaaag
     1141 tactcgacta aacgtaggaa agagtgtggg acaaatctaa aaatcaattt ttgctacaga
     1201 ctttaattcc aaataaacag aacagaaaga ggtgttcttc tttctgttct gtccaaatta
     1261 ttctaagatt tctctttacc aacattattt gacaaagggc ctattaaatc taaaatagta
     1321 tctttagatt gctaaaatat gtgctagcaa atcattcatg gcttgttctt atttttaatt
     1381 ttatgcaata aaaaagaaga acaattagcg tgattataga aataaataat gagattttag
     1441 aaaaaaatgt gtatgaatac ctgattgtta ctttatttat tccactttca ctttttactg
     1501 taacaaaagg acctccttgt tttagtactg tagcattttt tgaattaaca gtaaccgtta
     1561 atcctttata ccggaataca ggtaaaacta ttgttttgcc ttttgaagat ttaaacgtaa
     1621 atgtaatttc atctttattt ggagaaaagc tactataaat aacttgacca tcttctgtaa
     1681 cttgatggtt tttcaaaagc aattgatctt ttttatagcc tataggtaca taatcaaaat
     1741 tctcaaattc ttcaagttta ttttccattt gttcattaga ataagttcct tgaaaattca
     1801 tttctttcac catgttaact actgatgaat aatttaaaat tga
//
LOCUS       DARBFO010000193         1836 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000193 DARBFO010000000
VERSION     DARBFO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.199"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..972
                     /locus_tag="SCK51_002967"
     CDS             <1..972
                     /locus_tag="SCK51_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG1253515.1"
                     /translation="LPINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARN
                     VAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDK
                     ALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGY
                     LSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFG
                     EIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGY
                     HVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1162..1235
                     /locus_tag="SCK51_002968"
     tRNA            1162..1235
                     /locus_tag="SCK51_002968"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1196..1198,aa:Ile,seq:cat)
BASE COUNT          606 a          317 c          346 g          567 t
ORIGIN      
        1 ttacccatta atacgtttgc ttatactatt aatgacgagt ataatttagc gccgaatcaa
       61 ggagactcca gattagcaat tcctaacaaa attattttgc acgaaactgg aatagatgca
      121 ccagcaagaa acgtagccgc caacatgaaa aataattata acggaagcaa ttcttatact
      181 acagatgtta ttggtgacgg tgggattgtt taccgtgtgg gtgagcaagg atatgtttcg
      241 tggggagctg gtaacgctaa tccttatgcg cctgtacaga ttgaattaca gcgcacatat
      301 gataaagcat tgtttgaaaa aaactatcga gcttatattg aatatacaag agatagtgca
      361 aaaaaatatg gaattccatt gactcttgat caaggaactt ctttatttac aaaaggaatc
      421 atttctcatt tgtgggtgac aaattatgtt tgggggaacc acacagatcc atatggttac
      481 ttatcgcaaa tgggagttag caaagaaaag cttgcttatg atttagctca tggatttacc
      541 gatgaaaatc caacaacttc tgaaaacaag cctgtcattg atccaacacg agctggtgca
      601 gctaatccta cgctgacaga tggaacaaat cacgcccaca ttgatcagtt tggggaaatc
      661 gaaaacgcaa acttgcatgt ggctggatgg cacattgcta actataaata cgagtatatt
      721 ttcattatgg actacaatac tgggaaagaa ttagctcgag taagagctga tggaatttat
      781 agaccagatg taaaccaagc ttataatact tctggaaatg ttggttatca tgtatctttc
      841 aatatgcgta attttcctag taagaaagtc tatgtaatga tgcgggcaac gaatgatcca
      901 gagggaaaca ctaaaggcgg tgcgcaagat ttccatgaca aacgttggta tttaaatatt
      961 cctaaacgat aaaaatagct cctcgttgag gagcagtaca taactatatt gacaactata
     1021 aaaattattc gataaaatag tgatgttatc gcatatcttc actatcaccc ataaatagtc
     1081 acactccaag ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat
     1141 ggctttatgt ggcaacaacc agtaccctta gctcagttgg tcagagcaga cggctcataa
     1201 ccgtccggtc gtaggttcga gtcctacagg gtacattaac gtagccattt gaatcgttct
     1261 gtgttagaat ttttttgaag agtattatac aagctaaagc ttttcttcat tgccactcaa
     1321 atgagtggct tttttatgta tccttttatg gattaatgaa aggatgtttc acatagttat
     1381 acttctgtat atttgaaaag ttttactttg atttttaaat agaaagacat ttgggttaaa
     1441 ttgtgagata ataataaaga agagtttaaa gcgcacccca aaccacttcc ccataagtgt
     1501 gttacgcttt aaactctttt atatttgaag ccattaaaaa gcataccata tttttgaaaa
     1561 aaagtgagaa aaaaggctta caattggagt ggtagttaat tagtgactta tttttgattt
     1621 tatagcactg atactataaa atatagatat catcatatta cacaatctta atactaactt
     1681 aaaaaatatc tcctttcaca agtatggtga taaaattcgt tccgggctac ctttttaggt
     1741 agcctacttt aatctttata cctttctgga tcaacgaaag tatactttat atagtcataa
     1801 cgccgatgat cgctacgtgc gtccggcacg tcagtc
//
LOCUS       DARBFO010000194         1796 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000194 DARBFO010000000
VERSION     DARBFO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.200"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="SCK51_002969"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="SCK51_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEG1253516.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          503 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DARBFO010000195         1570 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000195 DARBFO010000000
VERSION     DARBFO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.201"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            86..802
                     /locus_tag="SCK51_002970"
     CDS             86..802
                     /locus_tag="SCK51_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEG1253517.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            821..>1570
                     /locus_tag="SCK51_002971"
     CDS             821..>1570
                     /locus_tag="SCK51_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HEG1253518.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKK"
BASE COUNT          607 a          188 c          306 g          469 t
ORIGIN      
        1 gaggaagaaa cattatttca atgattaaga gtcgagagca caaacggtag acgatgttat
       61 ttttataaaa agatataatt tattaatggt cgataattat gaaaggaaaa atattcttaa
      121 aaaaaagagt ataggatgtc gtatgaaaat agtcgctata gtaccaatga agttaaataa
      181 caaaagattg ccacaaaaaa acacgaaagc ttttaccaat ggaaaaccat tgtgttatta
      241 tatactttca acacttttaa aagtaaaaca gattgataaa gtgtatgtct attgtagtaa
      301 cccagatatt aaagaattta taccagaagg tgtaacattt ttaaagagat ctagttcctt
      361 agatcaggat acaacgagta ttacacaggt attagccggt ttcacaaaag aagtagaggc
      421 tgatgtatat gtaatgacac acacaacagc accatttatt agcgaaaaat ctattgaaaa
      481 aggactagat gccgtgctga caggagaata tgattctgca tttgctgcaa aaaaattgca
      541 agatttctta tggaaagacg gtgaaccatt taattatgaa ttggacaata ttcctagaac
      601 acaggatttg ccgcctttat acgaagaaac tagtggattt tatatttata atcgcaatgt
      661 gatgaccgag ttgcatagaa gaattgggaa aaaaccttgc atagttgaag taggtgagat
      721 tgagtcagtt gatattgatg aagccgagga ttttatgatt gctgatgcga tttataatca
      781 tcttatttta caaggtagtt aagacagggg gaaggttagg atgaacaatg tacagatttt
      841 agattgcaca cttagagatg ggggctattg taacaattgg gaatttggat ttgataatac
      901 aaaaaaaatt atttctggac ttatagaatc aggaattgag attatagaat gtgggtttct
      961 cacaaataaa acagtataca ataaaaatgt tacaaaatat acgaccttag aagaattagc
     1021 acagataatt cctgaaaaaa gagaaggaaa attatttgta gcaatgatga actatggcga
     1081 atataatata gaggatcttc cagattatga tggttcatct attgatggaa ttaggattgc
     1141 gtttcataaa aaaaattgca atgaagcgtt gaaattatgt cgtgagataa agaaaaaagg
     1201 ctacttagtt tttgttcaag ctatggtatc tctcaattat acagatgaag agtttttagc
     1261 agtaataaaa aatgtgaatc agtttaaacc atacgctttt tatattgttg atagttttgg
     1321 aatgatgaaa ggaaaagatt taactcgtct tttttacatg attgagcata atctagaaaa
     1381 agatatacag ataggttttc actcacataa taacatgcag cttgcatatt ctaatgcaca
     1441 aaaacttata accgtacaaa caaatcgaag cctgattatt gattcttcgg tgtacggaat
     1501 gggaagagga gcaggtaatc ttaacacaga actatttgta gaatatttga atgataatgc
     1561 aggaaaaaaa
//
LOCUS       DARBFO010000196         1562 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000196 DARBFO010000000
VERSION     DARBFO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.202"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            131..505
                     /locus_tag="SCK51_002972"
     CDS             131..505
                     /locus_tag="SCK51_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1253519.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="SCK51_002973"
     CDS             complement(619..1248)
                     /locus_tag="SCK51_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1253520.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="SCK51_002974"
     tRNA            complement(1382..1454)
                     /locus_tag="SCK51_002974"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          536 a          232 c          271 g          523 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 ta
//
LOCUS       DARBFO010000197         1562 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000197 DARBFO010000000
VERSION     DARBFO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.203"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..294
                     /locus_tag="SCK51_002975"
     CDS             <1..294
                     /locus_tag="SCK51_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG1253521.1"
                     /translation="FLIDNNTMAYLFNNTWDIYFGKILFETINLPRYSQVGALPSYNS
                     SDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKSFLQKMFV"
     gene            307..>1562
                     /locus_tag="SCK51_002976"
     CDS             307..>1562
                     /locus_tag="SCK51_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG1253522.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT          542 a          267 c          322 g          431 t
ORIGIN      
        1 ttcttaatag ataacaatac catggcttat ttatttaata atacttggga tatctatttt
       61 ggtaaaatat tgtttgaaac tataaatttg ccaagatact cccaagtagg ggcattgcct
      121 tcttataaca gttctgatat tgagaatatc agtgttaagg ttcctgtaaa ggatgaacaa
      181 caaaaaatcg gtactttctt caaacagcta gatgatacga tcgctcttca tcaacgtaag
      241 ttagatttac taaaagaaac gaaaaaaagc ttcttacaaa agatgtttgt ctaggaggga
      301 agaagaatga aaattagtca ccgtgaggaa gcagaagtcg aagaacagtt gattcgtgta
      361 cttggagaag ggcataatca gtggacgtat cgtccggatc taaaatcaga agaagacctt
      421 tgggtcaatt tacgccaaaa aattatctca aataatcaag cagaactgaa tgattttcct
      481 ttgaccgata aagaatttga aacgatcaaa acggaattac tgctgcgaac gaaaacccca
      541 tttgatgctg cgaaatggtt aaaaggtgaa aatgggatgg ctcggatcac gattgaacgg
      601 gaagattcgc aattgggttc tgtctcactt atcctttatt ccaatcaaga cattggtgga
      661 ggaatctcta cttatgaagt ggtccatcaa attgcaaaac gtggaagtaa catagaagca
      721 cgggatcgac gttttgacgt caccttatta atcaatggat taccgattgt ccaaatcgag
      781 ctgaaacaag taactgcaaa agatggtgtt tatcaggcat ttaaccaaat caaaaaatat
      841 gcagaagaag gtatgtttcg gaacaatatc ttttcgacct tacagttgtt tgtggtttcc
      901 aatgaacaaa ccactcggta ttttgcaaat gccttaccga aagacttgca ccctaagttt
      961 ttatttagtt ggcgaacgaa agacaatgaa aaagtagaaa acttatatga gttttgtaaa
     1021 caagtgttaa atatcccgga tgctcatcgt ttgattgcgg attatacaat cgtcagtgaa
     1081 gatcaagaca ataaaaccct catggtgtta catccttatc aagtacatgc gattcaagcc
     1141 ttgtttattg ccgcaaataa acatcaatca gggtatgtgt ggcatgcgac tggttcagga
     1201 aagacgttga ctagttttgt atctactaag cttttagcac gaaaatcggg gatcgatcga
     1261 acgatcatgt tagtggaccg caaagattta gataatcaaa ccaccacaga gtttacaaaa
     1321 tttgcttcgg aatttaatac tgggatttct tccgggaacg ccaaagcgaa tagcttgatc
     1381 gttggaacgg gaagtgccaa agaattaagt gaaaccctgt tggcagatgc caatgcaaat
     1441 gttgtaatca ttacaacccg tcaaaaacta gatgcggcgt tgaaatatgc aaagaaacaa
     1501 gaagagaaaa aaggaaccaa tcgcttccaa aaattaatgg gccaacatat cgtgtttgta
     1561 gt
//
LOCUS       DARBFO010000198         1551 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000198 DARBFO010000000
VERSION     DARBFO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.204"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            132..344
                     /locus_tag="SCK51_002977"
     CDS             132..344
                     /locus_tag="SCK51_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253523.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="SCK51_002978"
     CDS             506..1024
                     /locus_tag="SCK51_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HEG1253524.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="SCK51_002979"
     CDS             1031..1543
                     /locus_tag="SCK51_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253525.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          611 a          181 c          241 g          518 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt a
//
LOCUS       DARBFO010000199         1551 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000199 DARBFO010000000
VERSION     DARBFO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.205"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1019)
                     /locus_tag="SCK51_002980"
     CDS             complement(<1..1019)
                     /locus_tag="SCK51_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1253526.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRV"
BASE COUNT          471 a          245 c          265 g          570 t
ORIGIN      
        1 acccgaacga atgccgctaa ttcgtgataa gaaaaatcaa ccatcttttc ccagatatct
       61 tgaaaagaat gggatgagag gtatcgatta ccacataaat aatttaccgc gtcagcatca
      121 tctaaatata gttctgcata ataacgatat gtttttctgc atagttctgg cgatacgttt
      181 ttatatactg taattttgct caaaatttgg cgaatagtcc gaggagaaat ttgctgatca
      241 tgtgaggaac gaaaaacaaa agaattttcg tccgtatttt gtgctgttaa cttaaaatag
      301 ttctccagaa atggcatcgc ttcttttggt attggtactt tattcagtct gttttctttt
      361 ctattcagat taagaatctg cttggtcaaa gatacatctt tacgtttaag ttctactact
      421 tcatggatct ttaatccgca atacagaaga atggcagtaa ttgcaaggtt cctcggtcta
      481 ttcttttctt caaagctagc ttctctgctt gtttgaaagc cttcaatcgg gataaacaaa
      541 tgctcaaaat ctttgaaaag ttctgcatcg atatatcgcg gagctgcttt ggttgtttcc
      601 ggtaaacttg tacccaaaag agaaatctct tcttcaatca gtcctttttg atacaaaaat
      661 cgtaaaaaat gatttaatga atattgcttc cttgaaatcc cgtaatagct attttcaaac
      721 aattgctccg tttcttggtt cgctgtagtt tggaacgaac gagtatgtcg atagatttta
      781 gctaagaaat tctgataatc ttgtttttct aaatggataa tcagctgatc atccacttct
      841 tcagaaaata cttttctcaa ataatcagta aacagatgaa aatcttttgc gtattcatag
      901 cgactgccta atgaaaggga agaacgattt tcttctaaaa actgttgaaa gacggaaggg
      961 agcaagtgtg tacgttgctt taacttttct tgcagttgtt ttctagtttt gctgtccatt
     1021 acaaattccc tccaatagtg ttcattttct atattttacc gcatttcatc aaatgaagat
     1081 agacagtttg tcttgatctt aggaagcaaa tttttaacta agtactagct aaaagatatg
     1141 aagtaagaag atacattttt agttatttta ttagtgcagt cattcttttg atcttttaat
     1201 tgatttatcc tctttttagc tatgtggtta cttcatgagc aatgaaatgg atttggttcg
     1261 ttctaatggc aggaggctct ttcttttatt tccgaaaaat tagaaaacat tttttcagaa
     1321 tcgattggct ctccatttat tcaataatgg tttgtctaac gatattgttg ggataattct
     1381 taaagaagta ttagaaatag ttggtacttc attactttac atgaagattt attctatggt
     1441 aggagcaatt atattgagaa ttaatccttt tttatataag ttgtttgctt attgatggat
     1501 tggataaatt aaaccatcac aacgagtgga aattatatga aatttttcat a
//
LOCUS       DARBFO010000200         1514 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000200 DARBFO010000000
VERSION     DARBFO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.206"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          577 a          172 c          309 g          456 t
ORIGIN      
        1 ttaatttgac aattaagaaa gtattaactt gttttgtgaa aaagagaatt tgaaaaacga
       61 ttggcgaaca aatgctcggt aggtatggta taaatacaga gctaattggt gtgatgaaat
      121 cagatattcg cgtgagattc agtaaagtag gagttcgaaa acagctaggt gacaaacagt
      181 attgctttgg tacttcggaa tggtttatca aattcttatc aacagttcac ttctctttat
      241 gtttgagtga tgattcgtta ggttattgct tggaaagacc ggatgagagt agaagagttt
      301 aattttataa agtgaaaaga ggtaatgtac gatatacgta gggctgaacc aatagaggat
      361 aagcaaacga tgttacaggc gcaaaattca tagcttactg ttttggagat aacagaagag
      421 gaaataatgc agaagtaagg cgaaaaaagg acggaagcct aacagcatat gaaataaaga
      481 aaaatattat aactactgat aaatcggtat tgagcaaagt actaatagga gtcaaaaaca
      541 ttcataagct atgagtgttt cttggctact tttttatttt tatgtatcta caaaagtttc
      601 caggggcaga acctgtgatt attaatggaa gaacctcaag tattgtctta aatcattgtt
      661 atgaaaatgg attaaaagag ttcatcaagg tgtgattaac aagttacttt tcattacatc
      721 gaatttttca gaagttgctt atattggtga tgatattaat gatttatcat gtatgacatc
      781 tgtgaagaag cgggaggaat agtagaatgt ccaaaaaatg ccacaagaaa tgagattgaa
      841 ttatctgatt ttattgtaac tcacagtggt ggtgacgaat ctgtgataga gtttattgag
      901 tggatattgg aatcgatata gtgtagtagt acagaaaaat tatcatacaa ttaatactag
      961 gcataagaat attggcatat tctagagtta aaagtaacta ttcgaaaagg ataaggttag
     1021 aattttttgt tttgccagtg gaagaatata aaaagggcat tggaaattaa tatattagaa
     1081 tgctaagcgg agaaaaaaat atatgaaaag tacgtccttg tgatgggatg gaaaaataca
     1141 cttatttcaa acaacgataa tataattatg tgaaccgaag tatactcgct tctaattcaa
     1201 tcctcaaatc ccgacagtcg cgacttcaaa aaagcgtata gttttaatgt atccccatat
     1261 gatccgaaaa atggtaattt acaattaaca gtttgatgat gttgaagatg tggaaatgct
     1321 gaaattacca tgatgagaaa cacattaatt aaagggaatt aattaactag aatataatct
     1381 gattcaattt gaattaagca aaaaaaggct gagttttaaa taatcgcgaa ttgtaaaatt
     1441 aagttagaaa aataaaaagg catttatggt acactcaaat tgtatttccg acgaaagaaa
     1501 acaaaggagt gtaa
//
LOCUS       DARBFO010000201         1509 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000201 DARBFO010000000
VERSION     DARBFO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.207"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            101..868
                     /locus_tag="SCK51_002981"
     CDS             101..868
                     /locus_tag="SCK51_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1253527.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="SCK51_002982"
     CDS             1060..1458
                     /locus_tag="SCK51_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEG1253528.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          561 a          189 c          282 g          477 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatg
//
LOCUS       DARBFO010000202         1503 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000202 DARBFO010000000
VERSION     DARBFO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.209"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            114..1169
                     /locus_tag="SCK51_002983"
     CDS             114..1169
                     /locus_tag="SCK51_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1253529.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
BASE COUNT          609 a          139 c          282 g          473 t
ORIGIN      
        1 tgtttaagtt gacatggaaa caatctttta aaaagcaaaa agatgataaa gttacttttt
       61 atgggatgtt ggtagaaaac aaattattat aaatattaga ggtataaaaa gttatgaaac
      121 acaaggtaag tgtgatagta ccaatttaca atgttgaaaa atatttaaga aaatgtgtgg
      181 atagtttatt gagtcagaca ttaaaagaga tagaaatcgt attagttgat gatggttcgc
      241 cagatgcttc gggggaaata gcagatgagt atcaaaaaaa atattctaat gtaaaaacaa
      301 tacatagaga aaatgggggg ttaggaccgg caagaaatac aggaattgag aatgcgacag
      361 gagaatatgt ggcttttctt gattcagatg attgggtaga aagtgatatg tatgaaaaat
      421 tatacttagt tgcgtccaaa gaaaatgctg atatagtagt tagtggacat tgtgattttg
      481 cgaatgacaa agctatggtg aaaaagcaac atcctttgag tgggaatgta tatgatacgc
      541 ctaatagtat taaaaatgta cgaaaaaatt tatttggaca tagtccaaaa gatcaagaag
      601 tagaggcctt cccgatgtca gtatgtatgt ctttgtataa taaaaatctg ctagataaat
      661 atagactaag atttgaaaaa attttatctg aagatacaat ttttaacttg aatgcatatg
      721 atgtcgcaaa taaaattgct tttacagaat atacagatta ttgttataga aaagaagaac
      781 aagagtctat tacaaaaagt ttatctccaa ataagcagac ggaatttaaa gaatttttaa
      841 taactctaaa agctatagca tcgaaagaag atttagaatg tcaaatcaga gcaaaacgta
      901 tggcaattga ttattgtagg ctttatgtgg gcttagttga tgatgcaaat ttgggaatta
      961 aagagaaaag aaagtatata aaaaaattta ttgaagatga gggattaatt caatgttgga
     1021 agggatatcc tacaaatacg ctaccatttc aacaaaaatt ttttcataaa atgcttgaat
     1081 gtaagtgtta tacaatattg ttgttgttaa gtagaattag aaagataatt aaaaatatga
     1141 ccagaaaaaa attgtttata atattataaa ctaaaagtat atcaaataat agaaattaac
     1201 aagttacaaa aaaatattat aaagaataat attttctaaa aagtaaacta tagtaatgtg
     1261 gattatcacc ttccaacagt atataaaatg agcgtaaaaa cggctatttt tgccttaaca
     1321 acgatttttt tgtatacaaa tacgatatgc tcattaatta ttggaatttg tagtgctgtt
     1381 taaattaatg aaggtgtaat tatatgagtg atcattttga aaaagtttgt tgagaagatg
     1441 tgcttctagt catgtgatgg gagaaaataa tttaactgtt agagtgaata gttaaattat
     1501 tct
//
LOCUS       DARBFO010000203         1451 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000203 DARBFO010000000
VERSION     DARBFO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.210"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..625)
                     /locus_tag="SCK51_002984"
     CDS             complement(<1..625)
                     /locus_tag="SCK51_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEG1253530.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="SCK51_002985"
     CDS             complement(784..1050)
                     /locus_tag="SCK51_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1253531.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="SCK51_002986"
     CDS             complement(1040..1396)
                     /locus_tag="SCK51_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG1253532.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          405 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg a
//
LOCUS       DARBFO010000204         1440 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000204 DARBFO010000000
VERSION     DARBFO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.211"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            105..1406
                     /locus_tag="SCK51_002987"
     CDS             105..1406
                     /locus_tag="SCK51_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1253533.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DARBFO010000205         1417 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000205 DARBFO010000000
VERSION     DARBFO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.213"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(150..1337)
                     /locus_tag="SCK51_002988"
     CDS             complement(150..1337)
                     /locus_tag="SCK51_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEG1253534.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DARBFO010000206         1379 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000206 DARBFO010000000
VERSION     DARBFO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.214"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(61..279)
                     /locus_tag="SCK51_002989"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="SCK51_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="SCK51_002990"
     CDS             complement(545..739)
                     /locus_tag="SCK51_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253535.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="SCK51_002991"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="SCK51_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          490 a          223 c          183 g          483 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctctta
//
LOCUS       DARBFO010000207         1372 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000207 DARBFO010000000
VERSION     DARBFO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.215"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            122..>1372
                     /locus_tag="SCK51_002992"
     CDS             122..>1372
                     /locus_tag="SCK51_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1253536.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          428 a          271 c          261 g          412 t
ORIGIN      
        1 gctctatact ttttgatgtt ggtgaacttc accaacatct tttttgtata aaaaaatata
       61 gaaaagacac cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa
      121 gatgtctcat actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt
      181 cgaggaaaat tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg
      241 aactcttctg tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat
      301 cattcgttgg gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac
      361 ttatctggag ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc
      421 ggatacctct gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat
      481 tgctcgctta aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac
      541 ttcttcggtc tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac
      601 tttgccaaag gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat
      661 gagttttatc atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg
      721 cttgccttat ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca
      781 attgattgtt attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa
      841 tgctcagttg atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa
      901 tcatcgagtg aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt
      961 agggaaaaaa ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta
     1021 tgaaaaacgg gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga
     1081 tcgactgtta gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct
     1141 ttatgcggta aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag
     1201 cggtctgcca gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat
     1261 ccaaaatgcg cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat
     1321 caaagtatta aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DARBFO010000208         1355 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000208 DARBFO010000000
VERSION     DARBFO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.216"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1355
                     /locus_tag="SCK51_002993"
     rRNA            <1..1355
                     /locus_tag="SCK51_002993"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DARBFO010000209         1339 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000209 DARBFO010000000
VERSION     DARBFO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.218"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1309
                     /locus_tag="SCK51_002994"
     CDS             <1..1309
                     /locus_tag="SCK51_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HEG1253537.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCN
                     HLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEK
                     AKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          569 a          102 c          226 g          442 t
ORIGIN      
        1 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
       61 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
      121 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
      181 gccaaattat tggagtaaag gaattggtac aagatatatt aaattgattt ttgaattttt
      241 gaaaaaagaa agaaatgcta atgcagttat tttagaccct cataaaaata atccaagagc
      301 aataagggca taccaaaaat ctggttttag aattattgaa gatttgccag aacatgaatt
      361 acacgagggc aaaaaagaag attgttattt aatggaatat agatatgatg ataatgccac
      421 aaatgttaag gcaatgaaat atttaattga gcattacttt gataatttca aagtagatag
      481 tattgaaata atcggtagtg gttatgatag tgtggcatat ttagttaata atgaatacat
      541 ttttaaaaca aaatttagta ctaataagaa aaaaggttat gcaaaagaaa aagcaatata
      601 taatttttta aatacaaatt tagaaactaa tgtaaaaatt cctaatattg aatattcgta
      661 tattagtgat gaattatcta tactaggtta taaagaaatt aaaggaactt ttttaacacc
      721 agaaatttat tctactatgt cagaagaaga acaaaatttg ttaaaacgag atattgccag
      781 ttttttaaga caaatgcacg gtttagatta tacagatatt agtgaatgta ctattgataa
      841 taaacaaaat gtattagaag agtatatatt gttgcgtgaa actatttata atgatttaac
      901 tgatatagaa aaagattata tagaaagttt tatggaaaga ctaaatgcaa caacagtttt
      961 tgagggtaaa aagtgtttat gccataatga ttttagttgt aatcatctat tgttagatgg
     1021 caataataga ttaactggaa taattgattt tggagattct ggaattatag atgaatattg
     1081 tgattttata tacttacttg aagatagtga agaagaaata ggaacaaatt ttggagaaga
     1141 tatattaaga atgtatggaa atatagatat tgagaaagca aaagaatatc aagatatagt
     1201 tgaagaatat tatcctattg aaactattgt ttatggaatt aaaaatatta aacaggaatt
     1261 tatcgaaaat ggtagaaaag aaatttataa aaggacttat aaagattgat tatataatat
     1321 atgaaaagct attataaaa
//
LOCUS       DARBFO010000210         1294 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000210 DARBFO010000000
VERSION     DARBFO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.219"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1244
                     /locus_tag="SCK51_002995"
     CDS             <1..1244
                     /locus_tag="SCK51_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEG1253538.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DARBFO010000211         1287 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000211 DARBFO010000000
VERSION     DARBFO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.220"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..848
                     /locus_tag="SCK51_002996"
     CDS             <1..848
                     /locus_tag="SCK51_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1253539.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          418 a          235 c          252 g          382 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttt
//
LOCUS       DARBFO010000212         1246 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000212 DARBFO010000000
VERSION     DARBFO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.221"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            200..742
                     /locus_tag="SCK51_002997"
     CDS             200..742
                     /locus_tag="SCK51_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253540.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="SCK51_002998"
     CDS             898..>1246
                     /locus_tag="SCK51_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEG1253541.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DARBFO010000213         1235 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000213 DARBFO010000000
VERSION     DARBFO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.222"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            3..1010
                     /locus_tag="SCK51_002999"
     CDS             3..1010
                     /locus_tag="SCK51_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1253542.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1022..>1235
                     /locus_tag="SCK51_003000"
     CDS             1022..>1235
                     /locus_tag="SCK51_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEG1253543.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEM"
BASE COUNT          486 a          161 c          235 g          353 t
ORIGIN      
        1 aagtgtttaa aggaaaaaca atattagtaa ctggcggaac tggttccatt ggaagtgaaa
       61 ttgttcgtca gcttttaaaa tatgaaccaa aagctatcag aatttatagt agaggtgaag
      121 aaaaacaatt ttatatgcaa caagagctct caaatcacac taatttgaga tttttaattg
      181 gtgatatcag agactctcaa aggattgatt atgcatgtcg tgatgtggat attatttttc
      241 atgctgccgc aatgaaacac gttccagcaa gtgaatataa tcctatggaa gctgtaaaaa
      301 caaatgtgat gggaactcaa aatgtaatag atgcagcgat caaaaataat gtaaaacatt
      361 ttgtaggtat cagtacagat aaagtagtga gtcctactaa caccatgggt gcgacaaagt
      421 tattgagtga aaaattaata atagctgcca atcagtataa aggaaaaaca gaaacaatat
      481 tttcttgtgt aaggtttgga aatgtcatgg ggtcaagtgg atctgttatt cctcttttta
      541 gtaaacaaat aaaaaacaat aaagatatta ctttgacaga tgttgatatg acacgattta
      601 tgatgtcaat accggatgct gcaagattag ttctgtctgc tgctatgaat tcaaaatttg
      661 gagaaacata tgtcttaaaa atgccggcaa tcaatatttt aactttagct caatcattaa
      721 taaaaatata taatgataca catgatgaaa aatatagagg tgaaataaaa gttgtaggta
      781 tcaggccagg agaaaaatta tatgaagagc taatgactac agacgaaatt gaacgctcat
      841 atgaaaatga taaaatgtat atcattccac cacactatca tgctactgac gaactgtaca
      901 aagattatga aaaaattaaa atcactgcat actcttcaga tgcaacagaa ttaatgagtg
      961 gacaggaatt tattagaatc gcaaaagaga ataatgtcgt gacttggtaa ggagttttat
     1021 gatgaaatca gaagtatatg taattgcaga aattggtgta aaccataatg gaagtctaga
     1081 tttagcttta gaaagtattg ataaagctaa agaatgtgga gctaacgcgg ttaaatttca
     1141 aacatttaaa acgaataagc tagtttccaa aaaggctcca atggcaaaat atcagaagga
     1201 taatcttaaa gaggacagtt ctcaatttga aatgc
//
LOCUS       DARBFO010000214         1224 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000214 DARBFO010000000
VERSION     DARBFO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.224"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>1224
                     /locus_tag="SCK51_003001"
     CDS             <1..>1224
                     /locus_tag="SCK51_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEG1253544.1"
                     /translation="SIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRACRS
                     CGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYF
                     VQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKIL
                     PRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEF
                     LVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKSQAE
                     KLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFF
                     HELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNGKIE
                     AKNTHIKTMKRVSYGF"
BASE COUNT          406 a          244 c          238 g          336 t
ORIGIN      
        1 tctatcaaaa aaatgctgag aataacagag aaagatttga tgattacaga ggtctcttac
       61 gagacccttc agaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc tactcctcgt
      121 gcttgtagaa gttgtggttc tactgtggta gatggaaacg ggaaagcaat tatagtgaaa
      181 aatggaaaaa aggaaacgat tgtccgtttt gaacaataca atcatatgcc tttggttatg
      241 cgcctaaaaa agcaacgcta tacctgtaaa aactgtcgca cccattggac ggctcaaagt
      301 tattttgtcc aacccagaca ttcaatcgca aatcatgtta gatataaaat tgcttcttta
      361 ctgactgaaa aagtatcttt atcttttatt gcgaaaagct gtcaggtatc tttgaccacc
      421 gttattcgta cattgaaaga gtttaaaagc tatttaccaa agcaatctaa gaagatcctc
      481 cccagagtat tgatggttga cgaatttcgt tcgcatgctt ccatagaaga taagatgagc
      541 tttatttgcg cggatggcga aacaggaaaa ttaatagatg ttttgcctac gcgtaaatta
      601 cctcgattaa caagctattt cttaggttgt accaatccag aagaagtaga attcttggtg
      661 acagacatga acgccgccta cttccagctc accaaacgtg ttctgccaaa tgcgaaagtc
      721 gtgattgatc ggtttcatat tgtcaaacac atgaatcaag cgttcaatga gttgcgtatc
      781 cgtgaaatga atgaacttcg taaagccgga cagaaaagcc aggcagaaaa actgaaaaag
      841 aactggcgct ttttgctaaa aaatcgtgca aacatcaacc attatgaata caaaacatgg
      901 aaaagtttcc gagcaccaaa atacccattt cttactgaag caatgatgat tgatcgattg
      961 cttgagtttt ctccgcccct aaaggaggcg tatccctttt ttcatgaatt agttgaagcc
     1021 tttcgagaca aagaccctga cttatttttc tccttattgg cagaacttcc cgaaacgtta
     1081 gatgacagct ttcgggaaaa gcttcaaaac cttctgacct atgaagaagg catcaccaac
     1141 gcaatgatct atccttattc caatggaaaa atagaagcga agaataccca cataaagaca
     1201 atgaaacgag tatcctacgg attt
//
LOCUS       DARBFO010000215         1195 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000215 DARBFO010000000
VERSION     DARBFO010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.225"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1076
                     /locus_tag="SCK51_003002"
     CDS             <1..1076
                     /locus_tag="SCK51_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEG1253545.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DARBFO010000216         1183 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000216 DARBFO010000000
VERSION     DARBFO010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.227"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>1183
                     /locus_tag="SCK51_003003"
     CDS             <1..>1183
                     /locus_tag="SCK51_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEG1253546.1"
                     /translation="LVFLLVPLYTSVLSTTEYGTYDLTISTATLLFPILTLNIVDAVM
                     RFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLLNITKLWQDVYGLEKYIFLYYISY
                     VWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANIFFLLILKWGLIGFFIANILSQII
                     SATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPLIATVVGWWINSTADRYVVSFIVG
                     VSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKEYGDNETAKFYGNTFVMINLLMCI
                     TCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIANVLNCASGLLGPILSAKKDSKSMM
                     WSAIIGALVNVIMSVVLVYKIGVQGATIATVICSYVIYVVRKRAVGKDIFIKHYHIVV
                     ES"
BASE COUNT          384 a          147 c          213 g          439 t
ORIGIN      
        1 tttagttttt ttgttagtcc ctttatatac aagtgtatta tccacaacag aatatggcac
       61 atatgatttg acaatttcaa cagccacatt attgtttcca attttaacct taaacattgt
      121 agatgctgtg atgcgctttt taatggataa agataaaaat aaaaaaaata tagcatctat
      181 agcgattaaa gttatatttt taagcgtgat agtctttgga tccgtaatgt atttgttaaa
      241 tattacaaaa ttatggcaag atgtttatgg attagaaaaa tatatttttt tgtattatat
      301 atcctatgta tggaatcaat ttctaataca atttgctaaa gggttagaaa aagttaaagc
      361 tatgggcata gcaggtatta tcagcacaat tgtaacaata attgccaata ttttcttctt
      421 gcttattctg aaatggggat taattggttt tttcattgca aatattttat cacagattat
      481 ttcagctact tatctgtcag tattagtgag aatatgggaa tatatcgatt ttgaggctaa
      541 agatcctcag ctaaagagag aaatgctaaa ttactcgatt ccattaattg caactgttgt
      601 tggatggtgg attaatagta cagctgatag gtatgtggtt tcctttatag taggtgtctc
      661 ggcgaatggc ttattgtctg tggcatataa aatacctcaa ataattaata ctttgcaggg
      721 aatatttata caggcctggc aaatttctgc aattaaagaa tatggagata atgagactgc
      781 aaaattttat ggaaatacat ttgttatgat taatttgtta atgtgcataa cctgttcaag
      841 tcttatcata ttaactagac cgttttcaca tattctttat gcgaaagact tttatgaagc
      901 gtggaaatat gttccatttt tattaatagc taatgtttta aattgtgcat caggtctgtt
      961 aggacctatc ttatcagcaa aaaaagattc taaatccatg atgtggtctg caatcatagg
     1021 ggcattagtt aatgtgatta tgagtgtagt ccttgtttat aagatagggg ttcaaggtgc
     1081 cacaatagcc actgttatat gttcatatgt aatatatgtt gttcgtaaac gagctgtggg
     1141 aaaagatatt tttataaagc attatcatat tgttgtggaa tct
//
LOCUS       DARBFO010000217         1160 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000217 DARBFO010000000
VERSION     DARBFO010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.228"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            14..1141
                     /locus_tag="SCK51_003004"
     CDS             14..1141
                     /locus_tag="SCK51_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1253547.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          419 a          200 c          224 g          317 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa
//
LOCUS       DARBFO010000218         1157 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000218 DARBFO010000000
VERSION     DARBFO010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.229"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>1157
                     /locus_tag="SCK51_003005"
     CDS             <1..>1157
                     /locus_tag="SCK51_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HEG1253548.1"
                     /translation="QKKKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVV
                     IDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGF"
BASE COUNT          379 a          229 c          226 g          323 t
ORIGIN      
        1 ttcagaagaa aaagacgttg atcgtcgatg ctgttttctc acctgctccc catacctgta
       61 gaaattgtgg ttctactgtg gtagatggaa acgggaaagt aattgtagtg aaaaatggga
      121 agaaggaaac gattgtccgc tttgaacaat acaatcacat gcctttggtt atgcgcttaa
      181 aaaagcagcg ctatacctgt aaaaactgtc gaacccattg gacggctcaa agttattttg
      241 ttcaacccag acattcaatc gcgaatcatg tcagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa attgtcaggt atctttgacc accgttattc
      361 gtacgttgaa agagtttaaa agctacttac caaagcaatc taagaggatt ctccccagag
      421 tattgatggt tgatgaattt cgttcgcacg cttccataga agataaaatg agctttattt
      481 gcgcagatgg cgaaacagga caattaattg atgtcttgcc tacgcgtaaa ttgcctcgat
      541 taacaagcta tttcttaggc tgcgctaatc ctgaagaagt aaaattttta gtcacagaca
      601 tgaacgccgc ctacttccag cttaccaaac gtattctgcc aaatgcgaaa gtagtgattg
      661 atcggtttca tattatcaaa cacatgaatc aagcctttaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagta ggacagaaaa gtcaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 ttcgagcacc aaaataccca tttcttacgg aagcgatgat gattgatcga ttgcttggct
      901 tttctacgtc cctgaaggag gcgtatccct attttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgacg
     1021 gctttcggga aaagctccaa aaccttctga cctatgaaga aggcatcacc aacgcgatga
     1081 tctatcctta caccaatgga aaaatagaag cgaagaatac ccatataaag acaatgaaac
     1141 gagtatccta cggattt
//
LOCUS       DARBFO010000219         1143 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000219 DARBFO010000000
VERSION     DARBFO010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.230"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="SCK51_003006"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="SCK51_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEG1253549.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DARBFO010000220         1136 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000220 DARBFO010000000
VERSION     DARBFO010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.231"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            143..400
                     /locus_tag="SCK51_003007"
     CDS             143..400
                     /locus_tag="SCK51_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253550.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(632..1063)
                     /locus_tag="SCK51_003008"
     CDS             complement(632..1063)
                     /locus_tag="SCK51_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEG1253551.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          435 a          144 c          144 g          413 t
ORIGIN      
        1 ttttctgtcc acgttcattt tactccaaag ttttcctcat ttcaaaagaa aatcacagaa
       61 tacacgaaga agcgtttcta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgataaagaa tttaaagaaa tcaatgactt attactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaatag
      481 attttaaatt tattgtccat aatgttatta tgaatttata gcattaagaa aagagataag
      541 actttacatt tttttaggaa tgtaaagtct tatctctttt cttaattgta caattagtat
      601 tatctgcact actttctaat taaataaatt tttagttatt aacttgatta aaatcagaga
      661 taatatcatt taattcagtt tgaagggttg taacgattaa atggaaattt tcttcatctg
      721 ttttattata tggggatgtt ttcgttatat ataacaactt ttctttttca attttcatta
      781 ataactctgt cgaagtctgt actttatctt gataattaaa tgcagacata ccactttgta
      841 taattgtaat tattgctgaa gcaattgaaa caatcaatag taaagacctg tgttcggaag
      901 cctgatcaat taatattggg atactagctg ataagactat ttttataata tttccgatac
      961 caattactct attataaaat cgaatttctt tttttaatct atcgattgta gaatcaatag
     1021 attcgataaa cgctttttga tccatcattt tctctccaat cattttatta aaatcattaa
     1081 caaaaatatt ataataggat ttaagtaagt tctaactaat cacatataaa aataaa
//
LOCUS       DARBFO010000221         1116 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000221 DARBFO010000000
VERSION     DARBFO010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.232"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..899)
                     /gene="vanY"
                     /locus_tag="SCK51_003009"
     CDS             complement(<1..899)
                     /gene="vanY"
                     /locus_tag="SCK51_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HEG1253552.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          401 t
ORIGIN      
        1 ttagtatgtg ttgatccggg aaacactgtc acaattacac cgtctatatt gtttcctgat
       61 atctcataac gaagattagt cggcacatga atggtggtat ttttagtaac aggataataa
      121 aagatctcat atttttcccc atttacacta acagaaatgg ttttttcttc ttttaggtaa
      181 tccatatatt cctcgagaac gaaattcttt tctttcataa tcgcactatg tggtaaacca
      241 acatagcgaa tatgccatgg ttcatattga attcctgtta actctgtttt gtcctctgga
      301 taacgtaaaa tgaacccgta tttccaagca ttttcttcta tccactttcc ttcaggggct
      361 cgttccattt tcgtcaagct tgatcctaca tctagtgata aacctgaatt atgctcacta
      421 taacctgctg gtaaggcata ctcagccccc atttcttggt aaagcacact ttgctcatca
      481 aagtctcgat agccactatt aataataaaa tgactaacgc caccctttac agcatcattg
      541 accatctctg aaaatttttg tgctatttct tttgacatat aaatattact atcaagcaac
      601 ccgtatccat ttattaattc gtcatgttta gataaattca cgatatctga cttcacactt
      661 tcttggcgaa caggatattt actattgatt aatagcagat ttccttgata aacctgttct
      721 tctgtaattg ttttctcttg ggtattttca gaagtcccac tattttctaa atgttctttg
      781 ggattttgat cataattttg aaattcgact ttttcctgag aaaacagtgc ttcattaacg
      841 tagtcataac ctaagtatat taagaataac aataacaata aaaaaaacaa cttcttcatt
      901 ttcagtctcc tccttaacta tatagttgga agtatgaaat atttctagaa tcttcccctt
      961 aaacggcaca atctcgtttg agattgtgcc gttttgcccc gtaaattaca gtcaattatt
     1021 tcgccctact ggatttacta ttatcaccaa tgtagatgaa ttttcataag tgatacccat
     1081 gctgaattta acgaggctga actgcctgtt gacaat
//
LOCUS       DARBFO010000222         1110 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000222 DARBFO010000000
VERSION     DARBFO010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.233"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(85..1041)
                     /locus_tag="SCK51_003010"
     CDS             complement(85..1041)
                     /locus_tag="SCK51_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253553.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          362 a          182 c          167 g          399 t
ORIGIN      
        1 ttacgtaaat ggaaagtttt gtctcgctta atttgacaaa taatattttt agaaaaagca
       61 gtcggacttt ctaaaagaag ttcactattc gatcctgtcg atgatttctt caggaaaatc
      121 gtctatttgc ttgtcaattt tgctgtcttt gtatttctct tcttggatag tcaacaaaat
      181 ctttgtaaga ttcccccagt ctactgctct tggcggatcg agccaaacca ctacttgaga
      241 tgcactttct tcatatagat tattcctatt cgtgattata acatccggga actcaggctt
      301 tcctgcctct atcacttccg tgttcagatt aatgtacaag cttaaatttt tcttgataaa
      361 ttgattatag gcagcaccat aagaaaaatc gatgatgatc tttactggtt cagaaatcag
      421 ttgaatgggt atgtgattga tcaataataa taaataactg aaaaaaagaa aaaatttatc
      481 ttgaagcaac tctttgcgtt ttgacgttag atcatttata tactctatca aatagcaata
      541 aaatgctgga tagttttctc taaaaaaaga aacatcaagt ggaaattcat cagctttatt
      601 aatagtgtaa tgcaaaagtt taaaatgtat tttggttaac tctcttcgga tcaattggct
      661 cgtctcttct cgcaacagat taaactgatt ttcaaaactt tcaatgaatc gttcattcaa
      721 ccattcgatt gtttcatctt cattttttct caagtatttt tgatccaatt tatcatgaag
      781 ataaagccaa gagagtgtct tattgataaa aataacggat ttttctccta atactccttt
      841 taagtggtaa aatcctatat ccgaaaaaaa tagcgactga ttgctagcaa tatctaaaga
      901 atcagcttgt tcagccaaca ggttgattgc taaaaagtgg aataatttct ttttttcata
      961 agcagacaga ggataatact gttttaaatc ttttaacatt ttttctgttt ttataattat
     1021 aggtagcgta tacaattcca caaaacttat ctctgttgag ttaaaaagat tatataaaaa
     1081 aagccgaatg tttttaggat tccctgtcaa
//
LOCUS       DARBFO010000223         1097 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000223 DARBFO010000000
VERSION     DARBFO010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.234"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..153
                     /locus_tag="SCK51_003011"
     CDS             <1..153
                     /locus_tag="SCK51_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEG1253554.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="SCK51_003012"
     CDS             159..575
                     /locus_tag="SCK51_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253555.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="SCK51_003013"
     CDS             681..944
                     /locus_tag="SCK51_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1253556.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1097
                     /locus_tag="SCK51_003014"
     CDS             944..>1097
                     /locus_tag="SCK51_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG1253557.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLGSV
                     AKFENQT"
BASE COUNT          416 a          139 c          223 g          319 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcggtt ctgttgcgaa
     1081 gtttgaaaat caaacgc
//
LOCUS       DARBFO010000224         1095 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000224 DARBFO010000000
VERSION     DARBFO010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.235"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            89..781
                     /locus_tag="SCK51_003015"
     CDS             89..781
                     /locus_tag="SCK51_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG1253558.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          486 a          123 c          164 g          322 t
ORIGIN      
        1 aaaaacagaa agtgtctaaa gaaagcttga cgaagaaaat aatttttaat aacttattta
       61 aggaaatttt ttgaaggaga agataaaagt gagaaaaaag attattatta atgctggagt
      121 aattatagga ttgatgttag taattttctc cggaaagcaa tatgctgata atattttatt
      181 atggggaggg gaagataata ttgaaaagat caacaacaat ttaactatat tagacacagc
      241 tctagaaaat aaagagcaaa aaattagtat tttaaattca caattgtctt caaacaacca
      301 aactcttgaa caactaagaa ctaatattga agtttccaaa caaaaagtgg catctttaga
      361 aaacgacaag aatcaattag tatcagagaa aactaattta gaaaatcaat tgtcagcaaa
      421 agactctgaa ttgcaaggaa aacaaaatga aattaatgcg aaaatagacg aaattaacca
      481 aaaagtagct gaaataaatc gattaaatag cgattggaat aatcgtttac aaaatgaaaa
      541 gaacaaattt aatgaagaac aatctactat taatagttta aaccagcaaa ttaatcaaat
      601 gttgagtgaa aaaaataaca ttcaaaatca actgcaagct actcagcaaa agctagaaga
      661 agaaaagtca aaaaatagta gtttgaataa ttacattgaa aagcttcaaa aagctaaaaa
      721 tgatgtagag gataccgcaa ataaagcaca acaaattgta aatgaacata caatccaata
      781 gatatagttt taatttaaca aatggtatgt ttggagataa tgagatagaa tataaatgtc
      841 ataatattat acaatctaaa caccgtttcc ttttctattt ttgatgacat cagtatatag
      901 ctactcgaaa gagtagtctt tttattattc tcgcttatta ttgatatgaa aatttcgatt
      961 tgacaaaagg taccgaataa ttttgtaata tgaattatta tcaagaagta attatgatga
     1021 taacatatgg aaactagtta gtaataataa caggtgatta aacttttgaa cgacttgtaa
     1081 ttctatattt tttct
//
LOCUS       DARBFO010000225         1069 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000225 DARBFO010000000
VERSION     DARBFO010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.236"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="SCK51_003016"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="SCK51_003016"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="SCK51_003017"
     tRNA            203..276
                     /locus_tag="SCK51_003017"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="SCK51_003018"
     tRNA            316..405
                     /locus_tag="SCK51_003018"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="SCK51_003019"
     tRNA            420..491
                     /locus_tag="SCK51_003019"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="SCK51_003020"
     tRNA            507..579
                     /locus_tag="SCK51_003020"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="SCK51_003021"
     tRNA            589..661
                     /locus_tag="SCK51_003021"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="SCK51_003022"
     tRNA            697..772
                     /locus_tag="SCK51_003022"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="SCK51_003023"
     tRNA            774..854
                     /locus_tag="SCK51_003023"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
     gene            861..934
                     /locus_tag="SCK51_003024"
     tRNA            861..934
                     /locus_tag="SCK51_003024"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Trp,seq:cca)
     gene            937..1009
                     /locus_tag="SCK51_003025"
     tRNA            937..1009
                     /locus_tag="SCK51_003025"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:970..972,aa:His,seq:gtg)
BASE COUNT          252 a          240 c          288 g          289 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg
      961 caccggattg tggctccggc attcgtgggt tcgattccca tcattcgccc tttttattct
     1021 tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcgt
//
LOCUS       DARBFO010000226         1051 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000226 DARBFO010000000
VERSION     DARBFO010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.237"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="SCK51_003026"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="SCK51_003026"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEG1253559.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DARBFO010000227          996 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000227 DARBFO010000000
VERSION     DARBFO010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.238"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(25..912)
                     /locus_tag="SCK51_003027"
     CDS             complement(25..912)
                     /locus_tag="SCK51_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEG1253560.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          322 t
ORIGIN      
        1 aatttctttg cctttttatc taaatcaaac gtttagttct tgaccaacta ataaagaata
       61 actggacata gacatgccat ttttcgccat tagatcatca acagacatgc caaatttttg
      121 tgcaatatcc catagcgtat cacctgacac gactgtatat gtctgaccat tatttgtatt
      181 tacatctgtt gttgaagatg atgacgttga agtcgtcgta gtagtcgttg ttgttgtact
      241 tgtggttgtt gtagccgaac ctgttgatgg cgtatcaaaa cgggttaaat tataagtatt
      301 aatcaatgag ttcaatttgg ccccataatt tggatcggta gcataacgac ctgtcaagta
      361 cgctgttgca tcataaaaac ttgttgtatt gcttttccac acgccagcat aatgataatc
      421 gcccgtggaa aaactggttg aacgaagaac attcgcatgg tcttggaagg attcccaata
      481 agaagaatat tttcggaatg gctcattcat cgttacccat tgtccgttta catattcttt
      541 ggttgccata taaacgacct gtccgttata gcttcctttc acaccgaata gattataatt
      601 tggcgcacta gctaatccgg aagtgccata tgcactttcc aacaacgctt gagcgatcat
      661 gactgaagca tacaagtcat tacttgcagc aacttctgac gcagaccagc cgatttgatc
      721 gataaacgct tgaggatcag cgactgtttg agatccttgt tcatctgcat gaacatgttg
      781 cggtaccgct gtaaccatca tcggcccaac taaaagactc gttccaatta aaggagcaat
      841 cgctttttta ttgatcacca tttttctttt aggtctatgg tgccgggcac tgcgggtctt
      901 cagctcttgc attcaaattc ctcctcaaaa taaaaaaact taataaaaag ttctcttctt
      961 aaaacaaact taattaatta aattatactt ttaatt
//
LOCUS       DARBFO010000228          986 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000228 DARBFO010000000
VERSION     DARBFO010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.239"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..704
                     /locus_tag="SCK51_003028"
     CDS             <1..704
                     /locus_tag="SCK51_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1253561.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          333 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataat
//
LOCUS       DARBFO010000229          982 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000229 DARBFO010000000
VERSION     DARBFO010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.240"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            349..982
                     /locus_tag="SCK51_003029"
                     /pseudo
     CDS             349..982
                     /locus_tag="SCK51_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          333 a          181 c          179 g          289 t
ORIGIN      
        1 atgtagttat atatttaaat ttttaccaga aagaaatgaa caccttcaat tatctaaatc
       61 tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt aaacgaatta ctattcatgg
      121 tcttagacac acacatgcta gcttgctttt agcaagctag tgcaaatatt aaagatgtgc
      181 aagagcgttt aggccactct tctattcaaa tcattatgga tctatatatt catattacag
      241 acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata taggctctat aatttatggg
      301 actgatgaat caaaattgat tcttcagtcc cattgttatt ttaggtatat gattcgtaca
      361 ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa ggaccctgcc aaaagtattg
      421 atggtggatg aatttcgttc gcacgcttca attgtggata aaatgagctt tatttgtgcg
      481 gatggcgaga ctggaaaact cattgatatt ttgcctacac gaaaattgtc tcgattaaca
      541 cactatttta gtcgctgtgc aaatcctgaa gcggtggaat atttagtgac agatatgaac
      601 gccgcctact tccaactgac aaaacgtgtc ttaccaaatg cgaaagtcgt cattgattgt
      661 tttcatatca tcaaacattt gaatcaggcg ttcaatgatt ttcgtgtaca agaaatgaaa
      721 agattacacc agttagggca acgacgggaa gccgagaaag tgaaaaagaa ctggcgcttt
      781 cttctaaaaa accgtcaaaa aatcaatcac tatgattaca aaacatggcg cagttttcgt
      841 gcacctaaat acccgctact cactgaagca atgatgattg atcgattgct ggcattttca
      901 acgcctttaa aggacgccta tacttttttc catgagctga ccgaagcctt tagagacaag
      961 gaatcagact tattttttac ct
//
LOCUS       DARBFO010000230          959 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000230 DARBFO010000000
VERSION     DARBFO010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.241"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(107..511)
                     /locus_tag="SCK51_003030"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="SCK51_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="SCK51_003031"
     CDS             complement(570..>959)
                     /locus_tag="SCK51_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1253562.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DARBFO010000231          948 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000231 DARBFO010000000
VERSION     DARBFO010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.242"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(109..>948)
                     /locus_tag="SCK51_003032"
     CDS             complement(109..>948)
                     /locus_tag="SCK51_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HEG1253563.1"
                     /translation="AMCKILNISRQTYYYQAKPIENESDLEEIVQEEFIRNRKAYGTR
                     KLKKCLAKRGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQ
                     EQPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYS
                     LTDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVH
                     QYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSG
                     EAA"
BASE COUNT          256 a          218 c          183 g          291 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgattcggc gccgactgag
      781 ttgaagccca cgctttgcta aacacttctt caattttcgg gtaccgtaag cctttcggtt
      841 tcgaataaac tcttcctgaa cgatctcttc taagtcggac tcattttcga tcggtttcgc
      901 ttgataatag taggtctgac gagaaatatt tagaattttg cacatcgc
//
LOCUS       DARBFO010000232          904 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000232 DARBFO010000000
VERSION     DARBFO010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.243"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            265..888
                     /locus_tag="SCK51_003033"
     CDS             265..888
                     /locus_tag="SCK51_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1253564.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          333 a          129 c          167 g          275 t
ORIGIN      
        1 tagttttaaa ataaactaat gtccttattt ttgtgttcac atttctgtta cctaagaaat
       61 acttaaagga ggtgatgtag ccagcaacga ctacaacttg aacccgctat ctacagttta
      121 tttgttgttt cttttgggta tactctaatg gtacaaatcc atatttgctt aaagcgttga
      181 tttttcagcg gtttttcttt attgcaaagt gtaccctaag acctgtatac taatagtaaa
      241 tagattggat aggaatgatg gcaaatgtat ttagcttatt taagagtttc ttccgacgat
      301 caaagtttag ctcggcaaca taagctaatt gaaaattggt cagtcaaaaa taatattgct
      361 gaagacgatt taaaaatttt cgaggaaaaa gtctctggaa aaaatattga aaacagacca
      421 gagctgcaag agcttatttc tttcatacgt gaacgagata cgattattat tcctagtctt
      481 gatcgattag caagaaatag taaagataca aaagacttac ttcaattgtt tcgtagtaaa
      541 ggtgcttcta ttgaaatatt agacctaccg tcgtttaatg gggtaactga ccctgcatta
      601 agagacttgc taacaaattt agtcatagaa gtgtttagct atgttgctga aaacgaacgg
      661 aaaaaaatca aagagcgtca aaaacaaggc attgaaattg caaagaaaaa tggtcgttat
      721 actggtaaaa agattcaata ccatgccaat gcaacaggaa aaaacaaatt gatttacgat
      781 gaaattgttg atggtctgaa gaaaaaagaa actatttcta aaatagcaaa gaacgcagga
      841 gttacaagaa aaaccgttta tcgagtaaaa agagatttag gaatgtaata tgttccagtt
      901 agaa
//
LOCUS       DARBFO010000233          885 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000233 DARBFO010000000
VERSION     DARBFO010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.244"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            56..>885
                     /locus_tag="SCK51_003034"
     CDS             56..>885
                     /locus_tag="SCK51_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HEG1253565.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          305 a          134 c          160 g          286 t
ORIGIN      
        1 tcccttctgt agaatagagt taccacaaca cattcacaga aaagaggact tccatatgaa
       61 cgattttact acagaaatta tggaaatact aatcaataaa ggtgatttgg atgatttgtt
      121 tcgtcgtcac ctagaattgg ctattaacgc tttattacaa gctgaattaa cggtatttct
      181 tgactatgaa aagtatgata gagctggatt taattcaggt aattcccgta atgggaatta
      241 ttcgcgctct tttaaaacag agtacggtga attgaattta gtaataccta gagataggaa
      301 tggagacttt tctcaacaaa ctttacctgc ttataaaaga accaatgact ctttagaaac
      361 cactattatt cagctagttc aaaaaggaat tactatgtcc gaaatctctg atctaatcga
      421 aaaaatgtat ggtcattatt acaaaccaca aactatttca aatatgagta aaattgtgtc
      481 tgaagatgtt tcggcattta aagaaagaac tttagaagct aaatactcag tcatttttat
      541 ggatgctact catattccct taaaaagaca aaccgtatca aaagaagctg tctatatagt
      601 aattggtatc aggttggatg gaactaaaga ggttctagga tttactatag ctccaactga
      661 atctgcttat gtttggaaag agattcttca agatctaaaa gaccgtggtt tagaagaggt
      721 tttgttagtc gtaacagatg gtttaagtgg tattgacgat agtattcata gtgtttatcc
      781 aaatgctcaa tttcaacaat gttgtgtcca tatatctaga aatatcgctc ataaggttcg
      841 tgttagtgat cgacaagaaa tctgtagcga tttcaaatta gttta
//
LOCUS       DARBFO010000234          871 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000234 DARBFO010000000
VERSION     DARBFO010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.245"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(211..609)
                     /locus_tag="SCK51_003035"
     CDS             complement(211..609)
                     /locus_tag="SCK51_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEG1253566.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(606..842)
                     /locus_tag="SCK51_003036"
     CDS             complement(606..842)
                     /locus_tag="SCK51_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG1253567.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          275 a          157 c          120 g          319 t
ORIGIN      
        1 ctaaaatata taaaaaatca gcccactagt tctaaaccac tatttcctac tattcactct
       61 tttgtattcc ttttaaatca attatttcaa aattaatcca tattttccat atttttatga
      121 aaaaactatt ttgtatgatt aaacataaaa aaatcagcat attccttaat gacatgctga
      181 tctaattgag aaataaagtt tttaattcgt tcattttatt gcggtttgtc gtaaataact
      241 ggttatcttg gactttagtg aatcgaaatc caatgaacta gtggtaatca ttatgataaa
      301 atcaacagct tcttgacgag aaaaagcaac tggacaatta ttcaattgaa gaaacaacaa
      361 catggcaaca agagcagttc gtttattgcc attatgaaaa ggatgttttt tagctaaatt
      421 aattgctaaa atcgccgctt tttcatagat ttcaggatag agttcttgat tgaatactgc
      481 ttgtgaaggc tgtttgacgg ccatatctaa tgcatttgca tcttttacac cgatcatttc
      541 gcctggcgag tgacgtaaaa taactttcgc gttgattttg ataatttctt tagtcgaaag
      601 atacctcata aatcaaccaa atcttttaac gcgtcttcat attgattcat gatcgaatca
      661 actaatgaat caatatcagg ttcttcttgt gctttgatca ttcgtacaga accatcagat
      721 tgaaaaacat aactgattgc gtcacctgtc tgcacaccca tgaattcttt tacttctggt
      781 gggatagttg ttacatcgct attgccagat tttctaattt tatacttttt catttttttc
      841 atatttcgcc accctctata ccattctcat t
//
LOCUS       DARBFO010000235          848 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000235 DARBFO010000000
VERSION     DARBFO010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.246"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..>848
                     /locus_tag="SCK51_003037"
     CDS             1..>848
                     /locus_tag="SCK51_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253568.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLT"
BASE COUNT          268 a          113 c          123 g          344 t
ORIGIN      
        1 atgaatttga aaaaaatttt ttctgtaaaa atatttatag tttatgcatt tgttagttta
       61 ttatatttat ttccagcatt taaaaacatg gaaatatttg gcttttatga tacggcattt
      121 cacattaatc gtgcgttaag tttagaatct atttttagct cccctattaa ttttgaaacg
      181 tttagatcat atggcatgca agtaaataat ttttacccat ggttaactct atatccttta
      241 tttttattga tcaagtttac taatttagca ataggctata acctttttct ttatattgtt
      301 acgctcatta ctctatttat atgtcattat gtaatgtatg aaattaccaa aaaacatgtt
      361 actagctcct ttttcgctat tatatataca acatcttctt ttaggagtgt agaaatattt
      421 cttcgagggg caatggggga attactcgca atgtcaatac ttcctcttat tttactcggg
      481 tttataaagc tttacgactc aaaaaaagaa tcttgggtta tgttggcgat tagcatgact
      541 ttattaattt acactcatgt tctttctgta gctatgacta tggtaatgat tataattgct
      601 ttaattattc aattttatcg gaaaaagaaa atagaaatct tcttaattat taaattaata
      661 aaagctgcta tagttacttt acttctttca ctagcgttta taggtcctat gattgagcag
      721 acattgtatc aagatctgaa tagaccttat gtggatatat tacaaaaaag agcaatttat
      781 ctgggtaaag agtttttgat tggaagtata gattcagaat tactatcatt tggtattggc
      841 ttaacttt
//
LOCUS       DARBFO010000236          823 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000236 DARBFO010000000
VERSION     DARBFO010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.247"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..509)
                     /locus_tag="SCK51_003038"
     CDS             complement(<1..509)
                     /locus_tag="SCK51_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253569.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRE"
BASE COUNT          263 a          136 c          123 g          301 t
ORIGIN      
        1 ttttcacgta cacgagcgac atctgcactt gtcacctttt ctttgatgat gacatcgtct
       61 acagcttgct gccagtttga tacattttta gtaaataaat cgttgacagc tgtctggatc
      121 ttcagttttg attctgcact atctaactgt gtcacgattg ctttcttctc atcttggatt
      181 gcaacagctt ctttaggcaa ttctgattct ttgatgtcgt aatcatctgc tgaaagtttg
      241 acagaggata gcttatttga caaacttgtg attgtttctt ctgttatttc agggtttaga
      301 aaaatttttt cttttgtact atacagcttg tctacttctt ttgataaaga agtcaaggtg
      361 tctttttgat tggtgatggc agattctgca taattgatcc gttgctgatg ttgccgctga
      421 ttattgatat ggtatccaaa caaacagatc gcaatagcac ctgcacctac cgccacagca
      481 gtcaatgact tgacgaatgg cttttccata gattcactcc taagtattga tgatagataa
      541 attaagatat aattcactta cataaaaatg acagcttttt atttaaaaaa acataattaa
      601 actaatatat tcacagaaat atttgtaaac aagttttccc tatataatca taccacaaac
      661 tgttttcttg actagggcaa tttacgattt aaaagtaaat aaaacattaa taagataaaa
      721 attaatcgat ttcattttta tttccataat tttcataggt gtttttaaaa gttacacaaa
      781 aaatagaata acaaaaaaat taacattgaa ttaataatag tta
//
LOCUS       DARBFO010000237          816 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000237 DARBFO010000000
VERSION     DARBFO010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.248"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            136..309
                     /locus_tag="SCK51_003039"
     CDS             136..309
                     /locus_tag="SCK51_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253570.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          274 a          131 c          103 g          308 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaa
//
LOCUS       DARBFO010000238          811 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000238 DARBFO010000000
VERSION     DARBFO010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.249"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..672
                     /locus_tag="SCK51_003040"
     CDS             <1..672
                     /locus_tag="SCK51_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1253571.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DARBFO010000239          756 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000239 DARBFO010000000
VERSION     DARBFO010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.250"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..734
                     /locus_tag="SCK51_003041"
     CDS             <1..734
                     /locus_tag="SCK51_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HEG1253572.1"
                     /translation="DKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRK
                     LMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKI
                     YLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQ
                     YQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIR
                     WYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          321 a           98 c          127 g          210 t
ORIGIN      
        1 cagataaata tagcaagata aagcaagaaa ttacagcgat tgtaaaagaa tcaagaaact
       61 cttatggtta tcgtagagtc actttagcgt taaagatgaa aggatataca atcaatcata
      121 aaacagttag aaaattaatg tcccaaatgg gacttacctg tcaaatccga ataaagcgat
      181 ataaatctta taaaggaaca gtaggaaaaa ttgccaagaa tgtgttaaaa cgaaattttt
      241 cagtagatac acccaataaa aaatgggtga cagacgtcac tgaattcaag ataaaaggaa
      301 gaaaaatcta tctatctcct atacttgatt tatttaacgg agaaataatt agttatagta
      361 tatcaactag tccaacatat aaactgattg aagaaatgct tcaacaagca attaaaaaaa
      421 agggaactga gggttcactg attctacatt cagatcaagg atggcagtat cagatgccac
      481 aatatcaaaa aaaattaaaa gagaataaca ttattcaaag catgtctaga aaaggaaatt
      541 gcttagataa ttctgtaata gaaaattttt ttggtgtgct taaatcagag tttttttatc
      601 gagaaaaatt ccgatcaatt gagatatttc aaagtaaatt aaatgagtat attaggtggt
      661 ataataacaa aaggataaaa ctaaagttaa atggactatc tccagtagaa tatcggaaac
      721 agtccattaa atagttcaac ttttggggtt cagttc
//
LOCUS       DARBFO010000240          749 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000240 DARBFO010000000
VERSION     DARBFO010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.251"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(75..434)
                     /locus_tag="SCK51_003042"
                     /pseudo
     CDS             complement(75..434)
                     /locus_tag="SCK51_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          215 a          138 c          152 g          244 t
ORIGIN      
        1 tagtgacccc caaaagttag acttttttgg agtggagaaa tccactcttt ttttcatgct
       61 ctaaaatgat atttttacac aaccgtaacg gttgttttac gaaattggac gggactttgc
      121 cagtttaatt tctgttttat ccgttcatta ttgtagtaat agatatagct atcgatggct
      181 gattttaatt cctcgaaaca gttataaact ttcccgtgaa aaatctcttg ttttaataag
      241 ccaaaaaaat tctccataag tgaattatct aagcagttcc ctttccgaga catgctttga
      301 aagattttgt actccttcaa tttgtttcta taagctttca tttgatacga ccaaccttga
      361 tctgagtgaa acgtacgacg atagggacaa tcgttagtgg tctggatagc ttcctccaaa
      421 ccttccatta ccgcacgtgt ttctgaatgg tggtagcgac cgatttgaac ggctagggcg
      481 gaaatacggt gcaaatgcac ggtggtttca tgcaatgctt acagggcgta ttttgtaagg
      541 gtatgtaacg gagataatcc cgacggtaat ggatgtgaga ggccaagccg acgctgacgg
      601 tagggcaaac aaacatagtt gcactctagc gatcgttatt tatttttaac ggtcaaacta
      661 gggtacaact atgcttaaaa cacttcgcct caacttctgg tagcaaaagt gattttgaaa
      721 gtcaaaatat tttttatgag ttatttata
//
LOCUS       DARBFO010000241          733 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000241 DARBFO010000000
VERSION     DARBFO010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.252"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>733
                     /locus_tag="SCK51_003043"
     rRNA            <1..>733
                     /locus_tag="SCK51_003043"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DARBFO010000242          712 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000242 DARBFO010000000
VERSION     DARBFO010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.253"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..446)
                     /locus_tag="SCK51_003044"
     CDS             complement(<1..446)
                     /locus_tag="SCK51_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1253573.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DARBFO010000243          703 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000243 DARBFO010000000
VERSION     DARBFO010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.254"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          297 a          106 c          122 g          178 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaaaag ttattgacat gttactaacg acatgataag ataactgagt cgttaaaaaa
      301 gaaccacttg cgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa
      361 caaacgataa gttatgataa atgagttgcc gaaagatttc gaaaaacgaa atcgaaaaaa
      421 acttttaaaa aagagttgac aatcgcttgt ctctctgata tgatataaaa gttgctacgg
      481 cagccaaaaa tgaatcggga aacactctcg atgaagtaga cctttgaaaa ctgaacaaag
      541 taagacaaac caaatgtgta gggcgtcttg attcaattca agacaacaaa catttttaac
      601 aagcaagcaa tatgctagca aacaaattga gcttaacaat cgcaagattg ttcgaacttt
      661 ttatgagagt ttgatcctgg ctcaggacga acgctggcgg cgt
//
LOCUS       DARBFO010000244          702 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000244 DARBFO010000000
VERSION     DARBFO010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.255"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>702
                     /locus_tag="SCK51_003045"
     CDS             <1..>702
                     /locus_tag="SCK51_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEG1253574.1"
                     /translation="IDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTRELQY
                     NVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCYRS
                     NQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIESGI
                     HEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFTES
                     GEKVRRRKLIANDILI"
BASE COUNT          260 a           94 c          122 g          226 t
ORIGIN      
        1 attgattttg atatgatttt actagtagcg tataaaattt taactgtaaa cagtaacgtt
       61 gcacgtaata ttgctagtaa tataagggcg atttatgtgg atgaatttca agatactaga
      121 gaattacaat acaatgtaat agctaaatta cttcaaaaca accctcaaat tcagtcaatg
      181 tttgttggag atattgacca agcaatttat ggatcattag atggtatagc taaatcagtt
      241 gaagaactag aaatgctaac tagtcacact tttcaatcaa agacgctaca tggctgttac
      301 aggtctaatc agagattagt agacttctac tctaattttc agagttgtcc ttacgagatt
      361 gaaagcagag ggaataataa aaacgaccgt ggttttataa gttatgattg caaagttact
      421 aaggaggatt tacccaacat aataaaaaat ataataaagg aaaagattga aagcggcatt
      481 catgaaaaag atatttgtgt acttgctcct caacactatc ctctgttttc actgggtgag
      541 tatttaaaga aagaattacc gtattgcaat tttgatgcta taaatatatc accaattaag
      601 gtaaataatc atagtttatt tttcaagtta gcaattttgt actttactga atcaggtgaa
      661 aaggttcgaa gaagaaagct aattgcaaac gatattttaa tt
//
LOCUS       DARBFO010000245          687 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000245 DARBFO010000000
VERSION     DARBFO010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.256"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(288..587)
                     /locus_tag="SCK51_003046"
     CDS             complement(288..587)
                     /locus_tag="SCK51_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG1253575.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          225 a          119 c           73 g          270 t
ORIGIN      
        1 ttttatttta cttattgctt tcattttttc aatctatatt tccaattttt aacttacaaa
       61 aatgcaacat taataaataa cgatagtttc ataaaataca cttttaatta acattattag
      121 tttatactag ctgtttttgt gttaattaaa ggataaaata caatataaag aaacatctgt
      181 ttctttattt agaataaaaa aatacagact ctgttcaaat aaatgaacct agcctgcatt
      241 atgagattga ctatactttt taattcattg agatgaataa ctcgttttta taaatgccca
      301 aaagtaatcg gtgccgaagc cattcctcga cctaattctt tttcaaattt tcctattgtt
      361 gatatctcca tataaaacgg tgatactggt ttagacaatt ctcccctgat tgctaacgga
      421 cgtaaactac gttccaaatt atttatgact ttaggataat actgaccttt ttctaagcct
      481 gtctttgcgt ctatcaaaag tatacgttcc ttttctttga tctctggatt taaaacgaga
      541 tcatataatt ttagtaccat gttttctatt tcttcacttt tagacatcac gatttcctcc
      601 caaaaatata tttcttccca ctacattcta taaacaaagt atacgctaca tctagatttt
      661 tttaaatcat tactggattg ttaaaac
//
LOCUS       DARBFO010000246          642 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000246 DARBFO010000000
VERSION     DARBFO010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.257"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            78..281
                     /locus_tag="SCK51_003047"
     CDS             78..281
                     /locus_tag="SCK51_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HEG1253576.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            281..631
                     /locus_tag="SCK51_003048"
     CDS             281..631
                     /locus_tag="SCK51_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG1253577.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          265 a           66 c          113 g          198 t
ORIGIN      
        1 gatatttaat attacaagca atcttaagaa attcgaaaag gaaaaaatga ttatgaaaaa
       61 gaaagaaaaa gaaagtattg aaacattgtg ttattctagg aatattagga acttgtctag
      121 ctggcattgg tacaggaata gacgttgacg cagctactta ctatggaaat ggtctttatt
      181 gtaacaaaga aaaatgttgg gtaaattggg gacaatcatg gtcagaaggg ttaaaacgtt
      241 ggggagataa tctttttggt agttttagtg gaggacgata atgacttcaa taaacattga
      301 tcacacaaaa aaagtcatac atgaattata caatagttta atgacacgac ctaatcagac
      361 gaaagcatta ttagatatta ctgatgtttt atttcaggta tataaaaaaa ttgatacagc
      421 aaaatatcct gaaattcttg taaataaatt agttaattat atttacatcg tgggttttga
      481 taataaaata cgctttattg ataatgatga aaaattatta atagaattag gggatatttc
      541 taaaaaagct ggaattaata gtaaatacaa agcaaacttt acagataaat cgcaatttta
      601 tagttattca gaaacaatgc ctcaacgata aaatatagta ta
//
LOCUS       DARBFO010000247          636 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000247 DARBFO010000000
VERSION     DARBFO010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.258"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          186 a          122 c          118 g          210 t
ORIGIN      
        1 cccactctct cgcaagtttc tgttctggac agccccaaga acactggggc gttggccgtg
       61 gggttgattc tccccctctt tcaaaagagg ctgaaagagg gggttagggg ggagttagtt
      121 ttgagacccc aagactgggg taggggttga ttttttccct tttaaatttt atgctactac
      181 tatttttagt agtgtattct ttatctgttg gctgtcggcg cttggtatgt tattcggaga
      241 cttcccgctt aggtttttga ccattttctc tcataagtgg cggtttttcc acgttgagag
      301 aaaatggtca aaaacctgga agtcggagaa tgcctttcat accaagcgcc gacagccctc
      361 cccccactta ttacatataa cctttaaata atctctaaat aaccctcaaa taatccttac
      421 taatactgaa acgtaaaaaa aataaacgga tttcaaacaa aatacgttta ttttttctat
      481 tgattttagc tcaaaatcga ttcaattcga gtgaaatttg cttcaatttt caattggttt
      541 ttgttcaaat tttgaaacat ttttgctcaa aaaatgagtg aatttgtttc aaaagaaaaa
      601 caaatacgat cgaaaaaaat ttgaactcat aacaat
//
LOCUS       DARBFO010000248          635 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000248 DARBFO010000000
VERSION     DARBFO010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.259"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..270)
                     /locus_tag="SCK51_003049"
     CDS             complement(<1..270)
                     /locus_tag="SCK51_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253578.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="SCK51_003050"
     CDS             complement(276..>635)
                     /locus_tag="SCK51_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEG1253579.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DARBFO010000249          620 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000249 DARBFO010000000
VERSION     DARBFO010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.260"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            378..>620
                     /locus_tag="SCK51_003051"
     CDS             378..>620
                     /locus_tag="SCK51_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253580.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIP"
BASE COUNT          243 a           76 c          116 g          185 t
ORIGIN      
        1 tagaaggtga tcaaaatggg agtaaacgaa atgatgaaag tcagattgag tttttaaatg
       61 ataccttcct cagaacttca aaacgaattc aaagattcgt tgacaaatct tgagcaagat
      121 catttaaaaa aatattttct cttcatttga cgaaaaatgt ttagaaatct tatataagtg
      181 aagataaagt aattggtgta attaattgta gagaatagcc tttgagcaat tgaagcgata
      241 caattattta gaaaatgaac ttcctccagc atactacatg attccttacg ggacgatgtt
      301 aaagaagaaa gcaagaaatt ttagaagtat tagtagataa actaatgggc ttttctcaat
      361 ttaagggagt agcccaaatg tttatcaaat atggtgcaga tacagtagca accatgttta
      421 cagaagaata tcaagtagac tattatgaag taatagataa atgtgtacat tttgtttatg
      481 aaaaagtgtg gaattttatg aattttgcga taagtgaaga aatcttacga gtacaagaaa
      541 acagaacagg aatatttacc agagacagaa agcttaattt gattgcgcat tatatacgga
      601 atcgctggtc atcaatacca
//
LOCUS       DARBFO010000250          613 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000250 DARBFO010000000
VERSION     DARBFO010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.261"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            151..549
                     /gene="spxA"
                     /locus_tag="SCK51_003052"
     CDS             151..549
                     /gene="spxA"
                     /locus_tag="SCK51_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEG1253581.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          214 a           95 c          120 g          184 t
ORIGIN      
        1 taaccttaga taactttcat ttttttaaaa aaattatgaa aaaaaggtag ctatttaaaa
       61 gttgattatg tataatagaa acagatggga gattattatg atcttctgtt acaaaaaagt
      121 gattcttatc acgaaaagga gtggtgcacg ttgttaacat tatatacttc cccaagttgt
      181 acttcctgca gaaaggctcg tgcgtggttg caagaacatc agattccatt tgttgaacgt
      241 aatatttttt cagaaccatt gaacagttct gaattaaaag cgattttaca aatgacagaa
      301 gacggaacag aagaaatcat ctcgacacgt tccaaagtat tccaaaaact gaacatggat
      361 ttagatgatc tgccgttaca agaattattg gaacttgtgc aaaacaatcc tggattgctt
      421 cgccgtccaa tcatgattga tgataaacgt ctgcaagttg gttttaatga agatgaaatt
      481 cgtcggttcc taccgcgcga tgtacgacaa ttagaattac gtcaagcaca attgatggct
      541 ggattataga aggataatac gaaacttgga aaagactggc tctttgtcaa atgatgttgg
      601 taaagagaaa tct
//
LOCUS       DARBFO010000251          588 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000251 DARBFO010000000
VERSION     DARBFO010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.262"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          193 a          117 c           84 g          194 t
ORIGIN      
        1 aaccgggggc tttattcctg cagtccggct acgccttcct gcaatagaca aaactccaga
       61 tcgggtaatt cgcttgtggc gaaacccgac ctggagtttg tctctgtatt ttgaacccta
      121 aaaattaatc aaacagactg atattctcat aatcagcgcg aaataagcca ttaagtacag
      181 ttttcctacc tatttttgat aacccgctgg ttagttaata ttccttttcc gagagagata
      241 aatgattggt taaatctata tacatctcaa cctttaaaaa tgttttcgaa atacttcatc
      301 agtacatgtc atacaaatta ccagtgcatt ctcaaattct agttctttaa ttgatttcag
      361 ttgaggagca gttacttttg gcggaaattt ccatttttgt ccaaacctct tcccccgatg
      421 aaatttcttt gttgcaatta ttgcaatgaa aaatttttgt ttcaaaaata tacataatta
      481 cttcctccaa taaaaactat ctacctctca aattattttt ctctaataag aaaaacagaa
      541 aaggtaataa gatacaacag tgatgattcc taataaagaa aagactac
//
LOCUS       DARBFO010000252          526 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000252 DARBFO010000000
VERSION     DARBFO010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.264"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="SCK51_003053"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="SCK51_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEG1253582.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DARBFO010000253          514 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000253 DARBFO010000000
VERSION     DARBFO010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.266"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..132)
                     /locus_tag="SCK51_003054"
     CDS             complement(<1..132)
                     /locus_tag="SCK51_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEG1253583.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DARBFO010000254          500 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000254 DARBFO010000000
VERSION     DARBFO010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.267"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          168 a           96 c           58 g          178 t
ORIGIN      
        1 ttttaaaaat aagaatgcta aatcactcta caataacggc gaaagcacaa aaaataaaaa
       61 aattattacg ttattaaaaa aacagatgaa agatcaggtt ttattagatc tcattctttc
      121 atcacttgaa gggatttaat gaactacttt ttcctctttg tcgtttttat atattcgtat
      181 tccttcaaac agtaggatag cctctttcaa tgcttgatat ttgaactgtc cgttacttaa
      241 aaatttagat gtgccgccct cttcctagaa catattcttt tttacttttt cattaattga
      301 cagaccaatc gaacgaaact gtaaatttcg ataggataat gattggtcaa actcattcat
      361 tttcaagaca tctcgattca tattcaagat aggaatcttt ttatcaacat cactttattc
      421 gtattttcct ctaccataaa agcttcacgc tccacaaaat tgttttatac actactattc
      481 agcccattca acgaccaatg
//
LOCUS       DARBFO010000255          485 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000255 DARBFO010000000
VERSION     DARBFO010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.268"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..259)
                     /locus_tag="SCK51_003055"
     CDS             complement(62..259)
                     /locus_tag="SCK51_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1253584.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DARBFO010000256          462 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000256 DARBFO010000000
VERSION     DARBFO010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.269"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..430)
                     /locus_tag="SCK51_003056"
     CDS             complement(<1..430)
                     /locus_tag="SCK51_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1253585.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DARBFO010000257          452 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000257 DARBFO010000000
VERSION     DARBFO010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.270"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>452
                     /locus_tag="SCK51_003057"
     CDS             <1..>452
                     /locus_tag="SCK51_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253586.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DARBFO010000258          341 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000258 DARBFO010000000
VERSION     DARBFO010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.271"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>341
                     /locus_tag="SCK51_003058"
     CDS             <1..>341
                     /locus_tag="SCK51_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253587.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DARBFO010000259          338 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000259 DARBFO010000000
VERSION     DARBFO010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.272"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..338
                     /locus_tag="SCK51_003059"
                     /pseudo
     CDS             1..338
                     /locus_tag="SCK51_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          142 a           59 c           64 g           73 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcc
//
LOCUS       DARBFO010000260          331 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000260 DARBFO010000000
VERSION     DARBFO010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.273"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>331
                     /locus_tag="SCK51_003060"
     CDS             <1..>331
                     /locus_tag="SCK51_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HEG1253588.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQ"
BASE COUNT          114 a           60 c           68 g           89 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag a
//
LOCUS       DARBFO010000261          321 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000261 DARBFO010000000
VERSION     DARBFO010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.denovo.274"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(69..321)
                     /locus_tag="SCK51_003061"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="SCK51_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DARBFO010000262        38791 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.guided.015, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000262 DARBFO010000000
VERSION     DARBFO010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.guided.015"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            335..649
                     /locus_tag="SCK51_003062"
     CDS             335..649
                     /locus_tag="SCK51_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1253589.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            670..1047
                     /locus_tag="SCK51_003063"
     CDS             670..1047
                     /locus_tag="SCK51_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1253590.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1057..1830
                     /locus_tag="SCK51_003064"
     CDS             1057..1830
                     /locus_tag="SCK51_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253591.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            1852..3255
                     /locus_tag="SCK51_003065"
     CDS             1852..3255
                     /locus_tag="SCK51_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG1253592.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3437..4621
                     /locus_tag="SCK51_003066"
     CDS             3437..4621
                     /locus_tag="SCK51_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG1253593.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4618..4908
                     /locus_tag="SCK51_003067"
     CDS             4618..4908
                     /locus_tag="SCK51_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253594.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            4905..5126
                     /locus_tag="SCK51_003068"
     CDS             4905..5126
                     /locus_tag="SCK51_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253595.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5168..5947
                     /locus_tag="SCK51_003069"
     CDS             5168..5947
                     /locus_tag="SCK51_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HEG1253596.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6014..6508
                     /locus_tag="SCK51_003070"
     CDS             6014..6508
                     /locus_tag="SCK51_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG1253597.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6564..7202
                     /locus_tag="SCK51_003071"
     CDS             6564..7202
                     /locus_tag="SCK51_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253598.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7274..7669
                     /locus_tag="SCK51_003072"
     CDS             7274..7669
                     /locus_tag="SCK51_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1253599.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            7653..10106
                     /locus_tag="SCK51_003073"
     CDS             7653..10106
                     /locus_tag="SCK51_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1253600.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10103..12130
                     /locus_tag="SCK51_003074"
     CDS             10103..12130
                     /locus_tag="SCK51_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253601.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12127..13149
                     /locus_tag="SCK51_003075"
     CDS             12127..13149
                     /locus_tag="SCK51_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEG1253602.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13166..14095
                     /locus_tag="SCK51_003076"
     CDS             13166..14095
                     /locus_tag="SCK51_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1253603.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14340..14456
                     /locus_tag="SCK51_003077"
     CDS             14340..14456
                     /locus_tag="SCK51_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEG1253604.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14472..15275
                     /locus_tag="SCK51_003078"
     CDS             14472..15275
                     /locus_tag="SCK51_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG1253605.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            15510..15746
                     /locus_tag="SCK51_003079"
     CDS             15510..15746
                     /locus_tag="SCK51_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253606.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            15753..17705
                     /locus_tag="SCK51_003080"
     CDS             15753..17705
                     /locus_tag="SCK51_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEG1253607.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(17777..18274)
                     /gene="dfrG"
                     /locus_tag="SCK51_003081"
     CDS             complement(17777..18274)
                     /gene="dfrG"
                     /locus_tag="SCK51_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEG1253608.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            18636..19676
                     /locus_tag="SCK51_003082"
     CDS             18636..19676
                     /locus_tag="SCK51_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG1253609.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            19774..19962
                     /locus_tag="SCK51_003083"
     CDS             19774..19962
                     /locus_tag="SCK51_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEG1253610.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(20020..20373)
                     /locus_tag="SCK51_003084"
     CDS             complement(20020..20373)
                     /locus_tag="SCK51_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1253611.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            20904..21326
                     /locus_tag="SCK51_003085"
     CDS             20904..21326
                     /locus_tag="SCK51_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1253612.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            21323..21553
                     /locus_tag="SCK51_003086"
     CDS             21323..21553
                     /locus_tag="SCK51_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1253613.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            22051..22251
                     /locus_tag="SCK51_003087"
     CDS             22051..22251
                     /locus_tag="SCK51_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG1253614.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            22278..23471
                     /locus_tag="SCK51_003088"
     CDS             22278..23471
                     /locus_tag="SCK51_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1253615.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(23539..25104)
                     /gene="guaA"
                     /locus_tag="SCK51_003089"
     CDS             complement(23539..25104)
                     /gene="guaA"
                     /locus_tag="SCK51_003089"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEG1253616.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25163..26086)
                     /locus_tag="SCK51_003090"
     CDS             complement(25163..26086)
                     /locus_tag="SCK51_003090"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEG1253617.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26282..26887
                     /locus_tag="SCK51_003091"
     CDS             26282..26887
                     /locus_tag="SCK51_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1253618.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27195..28211
                     /locus_tag="SCK51_003092"
     CDS             27195..28211
                     /locus_tag="SCK51_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG1253619.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            28352..29653
                     /locus_tag="SCK51_003093"
     CDS             28352..29653
                     /locus_tag="SCK51_003093"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEG1253620.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            29699..30361
                     /gene="deoC"
                     /locus_tag="SCK51_003094"
     CDS             29699..30361
                     /gene="deoC"
                     /locus_tag="SCK51_003094"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEG1253621.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            30382..30780
                     /locus_tag="SCK51_003095"
     CDS             30382..30780
                     /locus_tag="SCK51_003095"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEG1253622.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            30846..31928
                     /locus_tag="SCK51_003096"
     CDS             30846..31928
                     /locus_tag="SCK51_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1253623.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            32140..33702
                     /locus_tag="SCK51_003097"
     CDS             32140..33702
                     /locus_tag="SCK51_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1253624.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            33695..34828
                     /locus_tag="SCK51_003098"
     CDS             33695..34828
                     /locus_tag="SCK51_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1253625.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            34828..35784
                     /locus_tag="SCK51_003099"
     CDS             34828..35784
                     /locus_tag="SCK51_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1253626.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            35883..37049
                     /gene="deoB"
                     /locus_tag="SCK51_003100"
     CDS             35883..37049
                     /gene="deoB"
                     /locus_tag="SCK51_003100"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEG1253627.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            37073..37891
                     /locus_tag="SCK51_003101"
     CDS             37073..37891
                     /locus_tag="SCK51_003101"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEG1253628.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            37908..38615
                     /gene="deoD"
                     /locus_tag="SCK51_003102"
     CDS             37908..38615
                     /gene="deoD"
                     /locus_tag="SCK51_003102"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEG1253629.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        12830 a         6726 c         7949 g        11286 t
ORIGIN      
        1 ctactatacg taatttgttc aagagcaaaa aataatttat ttcttttctc agaggtgggt
       61 tattttacga gtaaaggaga cttgctttat cccactcaat tattagataa aatagaattt
      121 gattataata tttaaaatac ctaggtagtc gttgttttaa atatagtagg aatcggacgc
      181 tcagtacgga gcgtctattt ttttacccaa ttttgaaagg aagtgaactt atgaaaataa
      241 aaaatcaaga atcaaaaggt cgctctcccc cctttaaaac catcacacaa ataagattca
      301 ttcaaacata aaaacagaaa ggataggtaa aaatatggaa cttaaatatg tcattcccaa
      361 tatggaaaaa acatttggac aattagaata tgctggtgaa gggaacattg aacaacgtcg
      421 aataaatggc agaccaacca ttctctctcg tagttacaat ttatactcaa cgattcaacg
      481 agcagatgat attgttgtca ttcttccctc cgaagctggc gagaaacatt ttgaatccga
      541 cgaaaaagtc aaactcatca acccacatat taccgcagaa ggttataaaa ttggcaatcg
      601 aggatttacc aattacatta tgcacgcaga cgatatggtg aaagcataag aaaggaaagg
      661 tgaaaaaata tgagattagc agaaggtatt gtgattgaaa aagaccagac atttggcaca
      721 ttgaaatttt ccgccttgcg tcgtgaggtt cgtatccaga atgaagatgg tacggtttcc
      781 gaagaaatta aagaacgtac ctatgactta aaatcaaaag gacaaggacg catgatacaa
      841 gtcagtatcc ccgcttctgt tcctttgaaa gaatttgact acaacgcaga agtggaatta
      901 atcaatcctg tggcagatac cgtagcgacg gctacttttc gaggggcaga cgttgattgg
      961 tacgtgaaag cagacgatct tgtattaaaa ggacagacaa aacctcatca gaaatataaa
     1021 aaagaagcgt ccgaaagtga aaaataggtg ttgcttatga caaacacacc attgattttg
     1081 aaagaaattc cgaaggatga agccatttct tttattcggc aataccatta ttcaaagata
     1141 ctccctagat tgtgtaaata ctttctaggg attttttcag aagaaaaatt attaggcgtt
     1201 gtggaattag gctggggtac gcaaccccta caaaccatac gcaaactctt tcccgacagt
     1261 tcccttcaaa ccacagatta tttagaaatt ggaaagatgt gttttcttcc agaaatgaat
     1321 caaaccaatt attttggcag tcaagctctt tcagctctaa ttaagtggtt gaaagaacat
     1381 acggactgtc attttttata tacactggca gatggaattg agggaaaatg tggctatgtc
     1441 tatcaagcgt ccaacttctt ttactgcggg tatttcaaga caagtgtcta tcgtgataag
     1501 cagtcatggg aaaagataca ccctcgaagt gctcgccttt tattagagga aaatgctcgc
     1561 tttgaacagg tagagaaaaa gcattggctt tcccaggcat tttgcgaata caaagggatt
     1621 gagaaaatca atggacgcat gtttcgctat ctctatccgt tgacaaaaga agcgaaaaaa
     1681 ctgttaggac acactctcta tcgacgtcat tattatccga aagaaaaaaa tttacgcttt
     1741 gaaaagcgaa tcgcttatca gaaatatgaa gccatttctc aaccaacttt tgataaacag
     1801 gctcgtatct acaatacaca attattttga taggtggaaa ggaggatttt tatgccattg
     1861 cttcatgaca gaggagagcg aatcaaagcc aacgataagc acttggtcta tcattttgta
     1921 ctaatttcat tgctggtagt ttttctagtc gtcctactcc cttttcattg gagagcgtta
     1981 caatcggtga actggcaagc agttcagtta gatacatttt tctatcaact tcatacgcca
     2041 tacttttgga gcagtattct cactgctctt tttgtatgcg gacttatcag ctggctttat
     2101 tatcactatc gtatagattc tataaaacag ttagagcacc gtcaaaagtt agctcgtatg
     2161 attttagaaa atcattggta tgaagccgaa gaaactcaat cagagggatt ctttaaagat
     2221 ttacccagca agaaaaccaa acaacgaatc cgccattttc ctaaaatata ttatcgaatg
     2281 aaagatggac taatccatat tcatgtagaa atcaccttag ggaaatatca agatcagctt
     2341 ttgcacctag aaaacaaact agaaacagga ttgtattgtg aattagtgga aaaaacgttg
     2401 aaagattctt atgtagagta tgtcctactt tatgatacta ttggcaatcg aattggcatt
     2461 gaagacgtgg tagctcaaga tggacgtgtc aaattgatgg aatcggtcta ttgggagttc
     2521 gattctctcc ctcacatgtt aattgctggg ggcactggta gtggaaaatc ctattttatt
     2581 ctcacattga ttgaatctct cctgcataca aatgcaaagc tttatattct tgaccctaaa
     2641 aatgctgatt tggcagactt aggggcggtc atgaataatg tctattaccg taaagaagat
     2701 atgttggcat gtatcaatca attttacgaa gatatgattg atcgcagtga aaccatgaaa
     2761 caacacccta actataaaac aggagaaaat tatgcttatc ttggtttgtc tgccaatttt
     2821 cttatttttg atgaatatgt ggcaatgatg gatatgctag gtcgtgaaag taccaccgtt
     2881 attcataaac tcaaacaaat tgtaatgtta ggacgacaag caggcttctt tttaattctt
     2941 gcctgtcaac gtccagacgc aaaatatttt agtgatggga ttcgggacaa ctttaacttc
     3001 cgtgtggctt tgggtcgtat gtctgaaatg ggctttggta tgatgttcgg aagcgataca
     3061 caaaaacaat ttttcttaaa acccatcaag ggaagaggtt acgtggatac aggaaaaagt
     3121 gtgattagtg agttttatac accccttgta ccaaaaaggt acgatttctt aggagaaata
     3181 ggaaaagtca ttcaaaaaaa gcaatctgag cctgtaccgc acgaagtgaa aggtacaggt
     3241 tcagacttgc tttgattggt gtggcgttag ccacgccaat cctttagccc cctctatcta
     3301 acaggggggc acaaatacaa ctgaaaacag gcaattctca tcaaccaatg ttgatggtac
     3361 aaaggattgc ggaaaatatg aaaggtgtaa actcaagggt catagtttac atctttttta
     3421 gattggaggt ttgcgaatgg aaaacgaaaa aatttggatt aaggaattga aagaaaagcg
     3481 actggcttat ggcgtttctc aaaataaact ggcggttgct tctcatatta ccagacctta
     3541 cctatcggat attgaaacag gcaaggcagt ccctactcaa caggtgaaag aagatttact
     3601 gaacgctttg gaacgcttca atcccgataa cccattggag atgttattcg actatgtacg
     3661 gattcgattc cctaccaatg atgtggctca aattatcggt gaggttttac gtttgaatat
     3721 ggattatatg ctacacgaag actttggcta ttattcttat cccgaacact atcgtttcgg
     3781 tgatattgtt gtcctagtct ctcatgatgt aagtaaaggt gttttattag aactgaaagg
     3841 aaaaggctgt cggcaatttg aaaactttct attggctcaa catcggagct ggtatgattt
     3901 ctttcaagat tgcatggaac ataaaggtat tttcaaacgc ttggatttag ctatcaacga
     3961 taaaacaggg attttgaata ttccagattt ggcaaagaaa tgtaaacagg aagaatgcat
     4021 ttccgtcttt cgaagcttta aaaattatcg cagtggtgaa ttggttcacc gtgatgaaaa
     4081 accatacatg ggaaacacac tttatattgg ctcattaaaa agtgaagtct atttctgtat
     4141 atatgagaaa gattatgagc aatacgtgaa aaatgatgtt cccttagaag acgcagaagt
     4201 aaagaatcgg tttgaaattc ggttaaaaaa tgaccgagcc acccaagcca tgaaagattt
     4261 attggcacat caacaggcgg aaaaaacagc ctttgaaatt atcaatcgct atatccgctt
     4321 tgcagataaa gacgacacta aacgtcggag tgattggaaa actaatgaac gttgggaatg
     4381 gtttatcggt aaaaatcgtg gagcattacg cctaaccaca cagccagagc cttattcttt
     4441 tgaacgaaca ttgaactggt tacatcatca agtcgcccct acattaaaaa tcgcaagcat
     4501 tttagatgtt ctcaacggta caaccattat ctccaccatg attcaggaag ccaaactgac
     4561 agaaaaacat gagaaattaa ttgaacaaca acatctagcc atggaggatt tgattacatg
     4621 agccttttaa caaaagaatt aaagaagtta gggtttcagt gtggcattga gtttcaagcg
     4681 tatatacaaa atacagggaa atatacgtct ctcattattg agggaaaacg gcaagctggt
     4741 gataccatct atacctatga tttttacaag gtgagatttt ataataatta taccaatcga
     4801 gtaaccgtct atggagagca tttaacccct tttcaattat taaggcgtgt aaaaagctat
     4861 atctattatc gagaaaaata tcttaaggaa aggaggacaa tcacatgaat tttgggcaga
     4921 atttgtacaa ctggttcttg gataatgcac agtcattggt attgttagcg attgttgtca
     4981 ttggactgtt tttaggtttt aaacgagaat tttcaaaact cattggcttc ttgattattg
     5041 ctctaatcgc cgtcggactt gtgttcaatg cagctggcgt gaaagatgtg ttattaaact
     5101 tatttaaccg aatcattggt gcttaacaga tatttttcat gataaaatag gaagtatata
     5161 ttttgttatg aaaggattta tgaatatgtt tacaaatgat caatttaaat ctcttttaat
     5221 taaaacatta gaaagaaaga gctcttcaaa ttattatgaa ggaggtaacg agattgtttc
     5281 taaaatgaaa atatcagagg taactttaga tgagactgac gattttactt attataaagg
     5341 atataaaaag ggctggaata cgctttgcac atatcttaaa ataagagttc cttttgatga
     5401 tttagacttt tttgaaagtc ataaagattt gattacccag acagcaagct ccatatatga
     5461 taaacaaggt gataatgtat tagtggatac tattttagta ccacttccag agaattacga
     5521 agtaattaac tttagtcagt tgaaaattag tgatgtggtt tcacaagcta ttgaagacgc
     5581 tgagtctttt atgagtaatg gtgaatatca acgtgccttt gacagagttc acactgcttt
     5641 tcatggatat ttaattgaaa tactaaaaaa atatgaaatt acagttccta gagacgaaaa
     5701 tctatctaaa ttatatagta ggatacaaca attaattgaa aaagaaattc aacctactga
     5761 acttgctgat atagtgaaga caacgataag gtcatcaaat ggaatgatta gctctcttaa
     5821 tgaagcaaga aataggcatt cacttgcaca ccctaataca aatattattg gaaagagaga
     5881 agcaaaacta ataataggta tttcaagtac agttacagat tatattagcg gataccttga
     5941 taagtagcgt cattaactga ttgctacttt ttttatgact atttttataa aaaggatgtg
     6001 ataagaattg gaaatgaaag tctatgtggc aaatttagga cggtataatg aaggtgaatt
     6061 agtcggagca tggtttactc caccaattga tgaggaagaa atggcagaaa gaatcggttt
     6121 aaatgaggac tacgaagaat atgcaataca tgattttgag cttccttttg atgtggacga
     6181 atacacgccg attagtgaaa tcaaccgtct atgtgaagcc attcaagaaa tcgaaggtac
     6241 acctatttat aatgaactca aagaaattca aggcatgtgg tttagtagct tggaagaatt
     6301 actggaaaac aaagaggata ttcattgtta tagtgattgt gattcgatgg aagatgtcgc
     6361 ccgttattac gtggaagaaa caggacagct tggtgaagtc ccttccaatt tacaaaatta
     6421 tattgactac caatctttgg gacgtgatat ggaaatagag ggtaattacc tagttacctc
     6481 tcatggggta tttgaatatt gtcagtaagt ttaacaatcc gtgttataat taaattaata
     6541 aatagcacgg agaggtataa attatggtaa atgtatttga aaatcttaaa ctattaaaac
     6601 tagacatgaa agataaaggc tgggtgatag attcctttta ctttcgttac aaacagcaaa
     6661 attatattgt acttgttaag ctatttgaaa aagaggaaaa agttccagaa tatgctctgt
     6721 taaaactaga atttttaaag gaaaacgatt tttcagatat gctagctgtc tatgctaatt
     6781 cggtaaaatt atatacagac acgaaaacaa taagagaata ttttggtatc gaatacagta
     6841 gtaacttggg agatgtttta tttcaattta gccaaactct tgctcgattt attccgacag
     6901 aagttagtga gaagaaaaat gaagaccaaa aagaagccat gtgtttctca ttaagtcaaa
     6961 gtgattcaga agatcccaga aaaaaatatt gtttttcagt tagaagaaat cctttaaagg
     7021 gaaatggtga gctgggaaaa cgaagtcctt ttaatgacaa taaaacgaga ttgtatagac
     7081 ccagcttata tgaacgatta gggtcagata ccaatttaag tttcagatat tcaatgaatc
     7141 cagatgatga agaaactgat gaaggtatta ttgcaaaatg gacaaaaaat aaaattgaat
     7201 agttaaagca ttgaaagagc aatcacctat tttgattgtt ctttttctat ttttacggaa
     7261 aggacgtgaa gacatgaaaa aaataagaag ttacaccagt atttggtcag tagaaaaagt
     7321 gatttatgcg attaatgatt tacaactccc tttcccaatt acctttacgc aaatgacatg
     7381 gtttatcgtt tctttgtttg tcgtcatact ctttggcagt ttaccaccac tttctctgat
     7441 tgacggagca ctactcaaat acttagggat tcccattgtt ctcactgggt tcatgtgcca
     7501 aaagaccttt gacggaaaga aaccctatgg atttctaaag tctatcatta cctatgctct
     7561 acgccctaaa gtaacctacg ctggcaaaca aatacgatta aaaaaagaaa caatgaatga
     7621 atcgattacg attgtaagga gtgaacaact agatggcata tccgattaaa tatatggaag
     7681 ataatctcgt atttaatcat gatggcgaag tattcgctta ttatgaatta attccctata
     7741 attacagttt tttaagttcc gatgaaaaaa ttcaagttca tgataatttc tgtcaattga
     7801 ttgcccaaaa tcgtgatggc aagattcatg ctctgcaact agcaaccgaa tcaagtattc
     7861 gagccacaca ggaacaatcg aaaaaagaaa tcactggacg attaaaagag atagcttacg
     7921 aaaaaataga tgaacagacc gaagcgttaa tttctatgat tggtgacaat caagtagact
     7981 atcgcttttt tattggtttt aaactctacc tcaatgaaca ggaagtatcc gtaaaaaatg
     8041 ttgggaaaga aattcaaaaa gcatttgcgg aattctttca tggtgtgaat cacaaattaa
     8101 tgggcgattt tgtttctatg cctatggcgg aaatcgaacg cttttctaag atggaacagt
     8161 tgttagaaag taaattatcc agacgctttc agattcgtaa attggagaaa gatgattttg
     8221 gttatattat tgaacatctt tacgggcaaa caggtgtccc ttttgatgac tacgactacc
     8281 atttaccgaa acgatacgaa gataaaaaag tgttggtaaa gaaatacgat attctcaaac
     8341 ccactcgctg tttaatcgaa gaaaatcaac gctatttacg gattgaacag gaagatacca
     8401 ctacttatgt ggcgtatttt acaattaata gtattgttgg tgaattggat ttccctaata
     8461 gtgaaatttt ttattaccag caacagcagt ttactttccc cgttgatacc agtatgaatg
     8521 ttgaaattgt cgccaataaa aaagccttat ctaccgttcg caataaaaag aaagaactga
     8581 aagatttaga tagccacgca tgggaaagcg acaacgaaac cagtactaat gtggtggacg
     8641 ctttagaatc tgtcgatgaa ttagaaagta atttagacca aaccaaagaa tctatgtaca
     8701 aactaagcta tgtggtacgg gtatcagcaa agaatttgga cgagctgaaa caacgttgtg
     8761 atgaagtaaa agatttttat gatgatctgt ctatcaaatt agtacgacct tttggcgata
     8821 tgctaggctt gcataatgaa tttattccct caagcaagcg atatatcaat gactatattc
     8881 aatatgtcac gtcggatttt ttggctggtc ttggtttcgg tgctacacaa atgattggtg
     8941 aaaattctgg tatttacgtc ggttacaatc tcgatacagg cagaaatatt tatctccaac
     9001 ctagccttgc aagtcaagga attaaaggct ctgtaacaaa tgcattagcg tccgctttta
     9061 ttggttcatt aggtggcgga aaatcattct ctaacaatat gctggtctat tattcagtac
     9121 tctttggcgg acaggcaacc attgttgacc caaaagctga acgtggtgaa tggaaagaaa
     9181 ccttacctga tattgcccat gaaatcaata ttgttaatct cacgagtgag aacgacaata
     9241 aagggttact tgacccttat gtgattttaa aactcccgaa agattcagaa agccttgcca
     9301 ttgatattct cacgttctta acaggtattt ccagccgaga tagcgagaag tttcctgtcc
     9361 tgcgtaaagc tattcggagt gtaactcaaa gcaaacaacg agggttgtta ttggtgattg
     9421 atgaattgcg aaaagaagat acgcccatca gcaatagtat tgctgaccat atcgaaagct
     9481 ttgtcgatta tgattttgcc catctgttat tcagtgatgg cagtgtcacc caatccatta
     9541 gcttggaaaa acaactcaat attattcaag tggcagattt ggtcttaccc gattccaata
     9601 gctcttttga agaatacact accatggaat tattaagtgt ggcaatgctg attgtcatta
     9661 gtacctttgc tttagacttt atccactctg accgttccat ttttaaaatt gttgaccttg
     9721 atgaggcttg gagtttttta caagtggcac aaggaaaagc tctatctatg cgattggttc
     9781 gagcaggacg tgccatgaat gcaggggtct atttcgttac tcaaaacgcg gacgatttac
     9841 tagatgaaaa gatgaagaat aatattggct tgaaatttgc ctttcgttcc actgacctag
     9901 tggaaatcaa aaagacctta gaattttttg gtgtggacaa ggaagatgaa aacaatcaga
     9961 aacgcttacg tgaattagaa aatggacaat gtttagtttc agacatctat ggtcgtgtag
    10021 gggttatgca gtttcacccg atttttgaag agttgcttca tgcctttgat accagaccgc
    10081 ctgtgcgaaa tgaggtggaa gaatgacaca aaaacaaaag aaatttcttc actatagttg
    10141 gataacgctc ctttgtgtgg gcgttatttt acttttatta ggcacgctgg gcaatgcagt
    10201 acaagcgaca ggcttagttg atgaaaccat tgatacaagc aatgaatact ccaagtatgg
    10261 tttgaatcaa tatcagcttg attattatgt ggacaatagc tggggctggc ttccatggaa
    10321 ttggagtgat ggcataggac aatcggtgat gtatggattg tatgccatta ctaattttat
    10381 ttggacaatt tctctttatc tatcgaacgc cacaggttat ttggtgaaag aagcctatgc
    10441 cttagatttc attagtcaaa catcccaagc aattggcgaa aacatgcaga cgattgcagg
    10501 cattacaccg aatggctttt ccagttcagg gttttatgtc ggtttcctcc ttctttttat
    10561 tctcattgtt ggaatctatg ttgcctatac aggattggtt aagcgagaaa cgacgaaagt
    10621 gatacgagct gttttaaact ttgtcgtggt gtttattcta tcggcttcct ttattgcgta
    10681 tgcacccgat tatattgcga aaatcaatga tttttccaaa gatgtcagta ccgccagtct
    10741 gaatattggg acaaaaattg ttcttcctaa cacggatagt caagggaaag acagtgtgga
    10801 catgatacgt gacagcttat tctctgttca agtaaagcaa ccatggctat tgcttcaata
    10861 tggctccact gatgtagaag cgttagggga agaacgtgtg gaaagtttat tgtctaccag
    10921 tccagataca aacaatggtg aagatagaga aaatgtagtg attgaagaaa ttgaagatca
    10981 tgacaatacc tatttaaccc taccgaagac gatttccaga ttaggaacag tcttcttttt
    11041 gtttgtattc aatattggta tctcgatttt tgttttcctt ctaacaggaa tcatgatttt
    11101 ttcgcaagtg ctatttatta ttttcgctat gtttttgcca atcagtttcc tattaagtat
    11161 gttgccgagt tttgatggca tgggcaggca tgccattacc aagttattta atgtgattat
    11221 gacaagggct ggtattacct tgattattac cgttgccttt agtatttcca ctatgcttta
    11281 ttcgctgtca gcaagttctc cctttttcat gattatgttt ttacaaattg tgacctttgc
    11341 agggatttat tttaaactgg gcgatttaat gagcctattc actttacaaa gcaacgactc
    11401 tcaaaacatg ggcagacaag tgtttcgcag acctcgtcaa atgatgaatc gacaaaccag
    11461 aagatttcaa cgcagtatga gtaagatttt tcgtggaaaa gcagggtcaa cgaagcaaaa
    11521 gacaatgaca acaaagcaga atcaaaccaa taaagctaac catactcgtc aaaatgaacg
    11581 gactacacca aagaaagaaa cagcactttc caatgtgaaa aagcaacaag gcggtatcgg
    11641 gcaacgatta ggtgaaaaga cagcccatgt attggacacg aaaaatcgtc tagtcaataa
    11701 aagaaagcag gcaaaacaac agatacaggc gacaccaacc catgtcaaat atgcccttca
    11761 tcaaggaaaa gagaatgtca ctcataatgt aacagacttt aaacaatcta tggcagatac
    11821 caaagctcaa cgcaaacaac agcgtatgaa ccagcaggag caaaaacgaa agaacattgc
    11881 tcaaaagcgt ttggagctgg ataaagcaaa agaaaaaaga cgaaaagaaa acccatcatc
    11941 tcctgttcag aataaccagc agcatgttcg caagcgtcag caaaccgtca cgcctgtttt
    12001 gtcacaaaac actaaacgtg aaaatcatca gcgaccattg aaaaaagagc aaaaacaaaa
    12061 gacaccaccg aagcaattca atcagaggaa gctaaataaa agaacggata agcgaggaca
    12121 acaaacatga agctaaaaat aacagtgatt tgtagtgtgg tcttctctct cttctttttt
    12181 ctcttattat tcctagtcat tttctttgca gatgatacgg acagtggcga aaacaacaag
    12241 gattcttcta ttccacaagg cggtgtgacc gtttcaccag aagtgttggc acaccgtccg
    12301 ttgattgaaa aatatggtaa ggaatatggg attgaagact atgtttctta tatcttagcc
    12361 attatgcaag tggaatcggg cggtacagca gaagatgtta tgcaaagttc cgaatctttg
    12421 ggtttaccgc ccaacagttt gagtaccgaa gagtccatca aacaaggggt taaatatttc
    12481 agtgaattat taaccagtgc agaacaacaa ggagtggata tagattctgt tatccaatct
    12541 tataattatg gcggtggctt tttaaattat gtgaggagtc atgggaaaaa atatacctat
    12601 gaattagccg aacagttttc taaagagaag tcgggtggtc aaaaagcaga ctatcccaat
    12661 cctatagcca tacctgtgaa tggtggttgg cgatataact atggaaatca gttttatgtt
    12721 cagttggttt cacaatacct aaccgacact tccccaacag aatttgatga tgaaaccgtg
    12781 caagtcatta tggacgaagc cttgaaatac gaagggtttc cctatgtctt tggaggggcg
    12841 tcgcctacca cctcttttga ttgtagtggc ttaatacaat gggtgtatga caaagctggc
    12901 atttctctcc ctcgtgtcgc ccaagatcag tatgacgcta cgcaagaaat ctctatggaa
    12961 gaagcccaag caggcgacct tatcttcttt cattcaacct ataatgcagg cacatatgtg
    13021 actcatgtgg cgatatattt agagggaaat cgcttttatc atgcaggcga cccaatcggt
    13081 tatggcgact taagcagtcg ttattggcaa gaccatctga ttggggctag acgtgttatt
    13141 cataactaag aaaggaatga atcaaatgat tataaaaatc gagcgaaaag aaaagaagga
    13201 gaaaccaaag aaatctaaaa aaccgagcaa gcaaagaaaa gttccgatga ttaaagttgg
    13261 cacgcataag aaattaacct ttgttttatg ggttttattg attggtagtg tcggatttgg
    13321 aatctataaa aattttactg ccattgacac tcatactgtc cgtgaaacag aaatcattaa
    13381 acagcagatt gtcgatacca accaagtaga aagttttgtg aaatcctttg cgaaagacta
    13441 cttttcgtgg caacaatccc aagaagccat tgataaacga aatgaaaaat tgacccatta
    13501 tttgacggaa gaattgcagg tattaaatga agaaatgatt cgtaaggata ttcctactag
    13561 ttctagtgtg aacgatatac aggtgtggca ggtttctcaa gtgaatgaga acacctttga
    13621 ggtactgttt tctgttgaac aagttattac cgaagacaaa gacaaggaaa ctatatcgtc
    13681 tagctttcat gtggttgtcc atatagatga atcagataat atggttatta tcaaaaatcc
    13741 gacaatgagt aagaaacctc aaaaatcgga ctaccaaccc aaacagctag aaagtgacca
    13801 tacagtagat acagaaacga tggacgaaat cactagcttt ttagaaacgt tcttccagct
    13861 ctatcctacc gccacagaaa aagaattgac ttactatgta agtaatcatg tgttacctat
    13921 gattaacaag gaatatgttt tcgaggaatt ggtgaatcct atctttacca gaaaagataa
    13981 ccaagtaatc gtgaatgtcg ctgtgaaata tttagatcaa gaaacaaagg caacccaaat
    14041 ctcgcaattt gagttgatat tagaaaaaca agataattgg aagattgtaa aataacaaat
    14101 attggtacat gattacagat actttgtaat catgtactct ttttgataaa aaattggaga
    14161 ttcctttaca aatatgctct tacgtgctat tatttaagtg actatttaaa aggagttaat
    14221 aaatatgcgg caaggtattc ttaaataaac tgtcaatttg atagcgggaa caaataatta
    14281 gatgtccttt tttaggaggg cttagttttt tgtacccagt ttaagaatac ctttatcatg
    14341 tgattctaaa gtatccagag aatatctgta tgctttgtat acctatggtt atgcataaaa
    14401 atcccagtga taaaagtatt tatcactggg atttttatgc ccttttgggt ttttgaatgg
    14461 aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat gcaggaaaaa
    14521 ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta ggaagcgtgg
    14581 acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga attacaattc
    14641 agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata gacacgccag
    14701 gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat ggggcaattc
    14761 tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt catgcactta
    14821 ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat ggaattgatt
    14881 tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta atcaaacaga
    14941 aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa caatgggata
    15001 cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa tcattagaag
    15061 cattggaact cgaacaagag gaaagcataa gatttcagaa ttgttctctg ttccctcttt
    15121 atcatggaag tgcaaaaagt aatataggga ttgataacct tatagaagtt attactaata
    15181 aattttattc atcaacacat cgaggtccgt ctgaactttg cggaaatgtt ttcaaaattg
    15241 aatatacaaa aaaaagacaa cgtcttgcat attaagtagt tcagttttgg agtacaaaaa
    15301 gtgaaaatgt aaacctaaat gagtatcaaa ttgcaactga atttgatact cgttttttat
    15361 tgttaaatca acgtttgttg ttaatttaca ccacaaaatg taagttaaaa tgtatataaa
    15421 aatgcgaacg tgaaataatt attgtaggaa tgcaaattgg tgtgttaaaa tggttacaat
    15481 ttactaagca aaaaggggtg cattttccta tgcaatttct tgagccaaaa aataagaacg
    15541 ctaagtctgt ggattgggaa atatcagagc aagtaagggt gatagttaag caatacgctg
    15601 aatatgcaga gcgtactgaa agcgaagcag tggatgaatt cttattgaac attttagacg
    15661 ataagaagtt tattgaatgg attgccaaca agcgaagtaa taagcggatt gttgaaaaaa
    15721 tgggtatcaa agatagagtg ggttgataga caatgaccaa gcttaaaaga ttagccacga
    15781 aggaagatga aattgtagat gttaaaattc cgatttcaaa cgaagaatta aaagataggg
    15841 ctaaacaata tgatttatta actccaaaac gatttgcaac aaggtataac aaaatgttgt
    15901 tcttgccaac gtcttttaaa tggaacggtt ctgaatatcc cattcaatat aattactgca
    15961 tcaatccgtt ttgttgtaat tttgggaaag aacagcacaa attcaaagat gtaaaaggga
    16021 aaccaagtcg atataaaatg actggtagtt ccaaagataa aggtcataaa ggtatgtact
    16081 gcaatgacaa tcctattggc agaggtgtat cgcaaaactg tacagtaacc cctttatcta
    16141 attggtctgt tgttgaagaa attaaacgcc ttatagaaat caatagcatc caagatgttg
    16201 agccagatta tcaatttcat aaagaaggct gctcggaaga agaatcaacc ccattcaatg
    16261 agccgaagca attttacaaa agggggaaaa gtaggggcaa atctcaacgc tatcaatgta
    16321 aggcgtgtaa gaagttcact aatgttctac caaaaagaga agaaaccact acttatcacc
    16381 aacaaaagaa caccattctt ccaatgttcg ctaagatggt tgttggaagg gtttcagtca
    16441 gtcgtacttg tgatatttta ggaattggag ttggaaccta ctatcataag ctggagtggc
    16501 tgtataggcg ttgtttggag tttttagaac gatatgagac acaacccctt caaacaaaga
    16561 aatttaacga gatgtggctt aatacggaca agatgcacta ctacctaaat aacgtccgta
    16621 agaaaggaca aggttcaaag aaatatacgg ggtttgaaga ccttaatatg caaacgtata
    16681 tagttgtatc tgctgaggtc ttatcaagat atgtgttccg ttctgatgtt gcctatgatt
    16741 ggaatatttc aatggatgaa ttaaatgaag atacaagaaa attcaaagaa gaccatctca
    16801 atacttttag tcgtaaaaat gataggttag attggtctta ttatccacaa gaaccatccg
    16861 ctaacgatag cgaaaatagg aatgcttatc ttcacgaatt agggaaaatt actaatagga
    16921 gtagatttgt cgatggttta aatgttgatg ctccttatac tacaactgct cactattggt
    16981 taattaaaca aatggttaat gccgacgaat ggcgaatgat tagtgatgat gacttctcta
    17041 ttagaaacgc cttttataga gtattcacga aagaattaag gttgagcgat gcacatcatt
    17101 ttatttgcca agttaacaag acgaaaagca gaaaacaatg cttaaaagaa ttcggacaag
    17161 caaaagcaga actgctcgat tggggagata tcagaggatt caaaacaaag tttctacgga
    17221 ctttggcatc tcattatcta actgaattac taacaagtca tcaattccac gaagaggcta
    17281 ttagtaaaga tggagaaaga tatcgcaaat atgccgataa ccctatcaaa catccattag
    17341 ccactaagga taaaggattt tattcagttg attgtagaac ggatttatct gctctggaac
    17401 caaacgaaat cgcaaagatg ctattaaatg ttaatgacca ttcgaccaat agctttattc
    17461 aacagataag acgttatata tcatctcttg aaagaccatt aacaacagct cgtggtgata
    17521 agaagagcta tatttacgct aacttcaatc cgaagtatgc tcaatttgcg attacaattc
    17581 taagaactta ctacaacttt tgtcgccctt ttaaatcagc ggataaaaag gttttgacac
    17641 ctgctcaaag attagggatt acagataaac aatttgattg gaaagatata atttatttca
    17701 aatgacaaaa agagcttgga gtaatcccaa gctcttgtgc tttatcttta ctgttcttct
    17761 tcaattaacg cacccgttaa ctcaataagt tttttctttc atatacatga aaatagtagt
    17821 tatacggatt tttatcattc tttatccctt tttgggcaaa tacctcattc cattcctcat
    17881 aattcacttc tggaaaaaaa gtatctcctt cgaattcatg atgtattttt gtgatgtaca
    17941 ttttctcaac ataagggaaa aacaaattat aaatctgttc tcctccgaaa ataaaaattt
    18001 cttcttcgtt tttacataac tcaaaaacat cttctattga atgaacaatt tcacaaccat
    18061 taaaggtaaa ccccttatct ctcgtcagaa taatatttct tctgtcaggt aaggctcttc
    18121 cgattgattc aaggttcttc ctacctaata ttatcggatg tccctttgta gtatttttaa
    18181 catattccca gtccttggga atcctccaag gaatgtcatt ctctttgcca atcactctat
    18241 tcttatccat cgcagcaatc aaagaaactt tcatttataa cacagccttt ttaactaatt
    18301 taccatctaa tttcttattt ctatcaaaaa accttttctc attatcctgc ccgttagttg
    18361 aataagcaat tttttctata agttatatta tcataatacc acaaaatcac aaattttcat
    18421 tcaattatca ttttcaattg cattttataa atcattttgt atgtaatttg gtatgtaagt
    18481 taaaatgaaa aaagtagtcc attttgaagt acattttgtc attttgtact ccaaaactga
    18541 actacttact tgcatatata cgcctttata gtggagtact acatttacga gattcggtta
    18601 gagtatcaga aaaagaaaaa ataaaagtta cagaaatgta tacttcaata aatggtgaat
    18661 tatgtaagat tgatagagct tattctggag aaattgttat tttgcaaaat gagtttttga
    18721 agttaaatag tgttcttgga gatacaaaac tattgccaca gagaaaaaag attgaaaatc
    18781 cgcaccctct actacaaaca actgttgaac cgagtaaacc tgaacagaga gaaatgttgc
    18841 ttgatgccct tttggaaatc tcagatagtg atccgcttct acgatattac gtggattcta
    18901 cgacacatga aattatactt tctttcttag ggaaagtaca aatggaagtg attagtgcac
    18961 tgttgcaaga aaagtatcat gtggagatag aactaaaaga gcctacagtc atttatatgg
    19021 agagaccgtt aaaaaatgca gaatatacca ttcacatcga agtgccgcca aatcctttct
    19081 gggcttccat tggtttatct gtatcaccgc ttccgttggg aagtggaatg cagtatgaga
    19141 gctcggtttc tcttggatac ttaaatcaat catttcaaaa tgcagttatg gaagggatac
    19201 gctatggttg cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa atctgtttta
    19261 agtacggttt atactatagc cctgttagta ctccagcaga ttttcggatg cttactccta
    19321 ttgtactgga gcaagccttt agaaaagctg gaacagaatt gttagagcca tatcttagtt
    19381 ttaaagttta tgcaccacag gaatatcttt cacgggcata taacgatgct cccaaatatt
    19441 gtgcaaatat cgtaaatact caactgaaaa ataatgaggt cattattatt ggagaaattc
    19501 ctgctcgatg tattcaagat tatcgcaatg atttaacttt ttttacaaat gggcttagtg
    19561 tttgtttagc agagctaaaa ggatatcagg ttaccactgg cgaacctgtt tgccagaccc
    19621 gtcgtctaaa tagtcggata gataaagtaa gatatatgtt caataaaata acttagtgcg
    19681 ttttatgttg ttatataaat atggtttctt attaaataag atgaaatatt ctttaatata
    19741 gatttgaatt aaagtggaaa ggaggagatt gttattataa actacaagtg gatattgtgt
    19801 cctatttgtg gaaataaaac aagactacga atacgagtgg atactatact taaaaatttc
    19861 cctttatact gccccaaatg taagaacgaa actttaatta atgttcaaaa aatgaatata
    19921 ataacaatca aagagccaga cgccaagacg cagagccgat aatttgagaa atgaaactct
    19981 catcttatcg gctctttttg tttatctgaa ttttactgac tagccttcaa tatttcttgc
    20041 ttcattgata ccttttgcta gtgcttccat taaggatagt tctttgtctg taaagctatc
    20101 catgtatttt tctacctgta accttcgagt gcttttaatc aaattatctg taggtaagaa
    20161 aaattcatca atagatacat ggagtaatga tacaagatca tacagcactt gtgtacttgg
    20221 gtgttgtcct ttgttttcta tatttgttaa ataacgtggg tcaatttcaa tcattgttcc
    20281 cacttgttca cgagtgagac cccgtttcat tcgagcctct ttaatcgcta aaccaacggc
    20341 tctaaaatcg tatttatctt cttttttacg catactaaat cacctctata cattttactg
    20401 ttcctatata accataaaca ggtatagaaa aacatgttat atagtttatg agttcctata
    20461 gaacactgtg gaatttatta atgcgatgaa tccaatttca aatagaaagg tcgctgattt
    20521 tatttaccta tacaacttta taaaatttag tcatactcct agaaagtaga taaaattggc
    20581 gacttaatgg agggaaaagt cgccatgaga aagataatct gtcatctagt tctcttaatt
    20641 aagttcttat caaaaaaact tatccacctg tactggatag atatgaccat caatgtggtt
    20701 ttgcagatta catagataga aaaatcaaag catatgaaca gagaaaacaa tctgtttatg
    20761 tgcttttttg gtcacttaat aaatatcttt taggatttct cttcatttat gcaacaaatc
    20821 ccccgttcgt gtcggggttt agtatgaagg gagataaaga agcacatgcc actccctttc
    20881 acgacagaaa gggggtgaaa agcatgaaac catcttcttt tcaagaaacc atagaaaatc
    20941 agtttgacta tatctgcaaa caagttattg aagatgaacg aaaagattac ttcaaacatc
    21001 tagacagact atcagcaaaa gaagtctcgt tttctgatat agataactac cttgtcaact
    21061 ctttttctac tgttgatacg tacgtcactg atttccaact atttaaatta tttggattaa
    21121 gaatcaatgt tgaaagtgat ttattaagtg aagccctgcg tcatctttca gagaaaaaac
    21181 ggaacattat tttacttcat tactttatgg atatgagtga tgtggagatt gcggagttac
    21241 taaatttgaa tcgttcgact gtttatcgtc atcgaataag tggactagcc atgattaaag
    21301 aattcatgaa ggagtgtaaa gaatgaaaac agcttatcca agggttccct tccccctcat
    21361 tgtaaaggcg acagatggcg acgtggaagc aattaatcag attgttaaac attatcgagg
    21421 gtatacctca aaacgttcat tacgtcgcat gacagacgaa tatggaaatt ctcatatggt
    21481 tattgatgaa accctacgtg gcagaatgga aacgaaactc atctctaaga ttttatcgtt
    21541 cgaaattaaa tagtcccatt ttttcgtgga agcgtgtcga atcgtatcca cgcttcccga
    21601 tttgggaggt ttgttattcc atcaaagcat attgtgtgtt cagtaggttt tgataggcta
    21661 acaagccaaa cgttctttga caaccaaata aaggcaatcg aatacgtttc ggtaagaaaa
    21721 gagctgatga actaacacgc catgacctac aaaattagtg agcgattcat gttagtaatt
    21781 cgaaagtaat ctttagcagg attgctgcca tgactttctt atcgtgataa tgatactccc
    21841 gtatagccat agtccgagcg ttagaagcgt cacaggcaat gggtacggct acatgagaac
    21901 catgcagaag tgaaattcct gtgagctttg ctagagctgt tcgattgcta agatattctt
    21961 ctcttgtcag tgtattatta taaaactatt tgatataatt ataggtgaat attggagttt
    22021 tcctagccta taattgggaa aggagtaata atgataacgg tcacaaaaaa ggatttagtt
    22081 gagctaggat atggaacttc tttttcagca gacatcataa aaaaagcaaa agaattgatg
    22141 attacaaaag ggcatatcta ctatcaatct cgaaagctag atagagtccc tcgtgaagct
    22201 gttgaagaaa ttctaggaat tcggttttca aatgaaaaca ggtctgagta gtttttgcac
    22261 agtaaggagt aaaaactatg gttaaagacc caataaaaaa agcaaagaat ggaacctatt
    22321 attttcgtgc aaatctaggt tatgattcta aagggaaaaa aattcaaaaa tatcgtagtg
    22381 gattcactac taaaaaagaa gcaagagaag aatattcgaa actgctattg atgaaaccag
    22441 aagaattaag tgagaataag gataaaatga cgtttaaaca ttatatcttt gaaattttcc
    22501 ttccatggta taagacacag gtaaagctca gaacatatga aaatcgctta ccaactatta
    22561 agaagcattt ctcttatttt gataaaatgg ctgtttccga tattgagcct attgatgtgc
    22621 aaaattggca actaaaatta tcaaagaagt gtaagtcttc ttatgtaaga gctgttcaag
    22681 gactattttc agttgctatg gatcgagcaa ttgtccttgg gataaccacc acaaatccat
    22741 ctaagattat tggcaatgtg aaaaagcaaa agtctaaaat tgaattttgg acaaaagaag
    22801 aatttgaaaa agtaatctct ctaatttaca aagaagatta ctatcaacac tttttattta
    22861 tctctctgtg gtttttattt atgactggaa tgcgaattgg tgaagctact gccattcaat
    22921 gggaagatat tgattttgac tcaggagtac taaccatcaa caaaacactc tattacaaga
    22981 atcaatcgaa ttatagattt gttgaaccaa aaacaaaggc gagcgttcgt catattgtat
    23041 tagataaatg cacattaact tatttatccg aatggaagga agtacaacaa aatctaattc
    23101 agacagactt tgtaatgagc tataacggta ttcctaccca aaaacataca ctatcatatg
    23161 ctattgaacg ctatgctaag ctggctggta tccatcgaat cagaatccat gggttacggc
    23221 attctcatgc ttccttgtta atcagtatgg gagaaaatcc acttatcatt aaagatcgtc
    23281 taggacacga agatattgag acgactttag gaacgtatgg acatctttat cctaacagta
    23341 atttcgaggt agctaacaaa ttaaatggag ccatttcctt taaagaagca aaacaaaatt
    23401 atgatacttc accaaagaat caatttactg tagactactt gcgaaacaaa gaaatgaaaa
    23461 aagtgcaata aaagtgcagt aaaaagataa gaagagccag aaacccttgg tataagagga
    23521 tttctagctc tcctacaact attcccactc cactgttgct ggtggtttag aagtgatgtc
    23581 atacacgata cggttgacgt gggctacttc gtttacgata cgtacagaga ttttttgcaa
    23641 gacttcccat gggatacggg caaagtcagc ggtcattccg tcgattgaag ttacagcacg
    23701 gataccgact gtgtagtcgt aagtacgtcc gtcacccata acaccgacag agcggatccc
    23761 tggaagaaca gtgaaatatt gccagatatc acggtcaagt ccagcagctg cgatttcttc
    23821 acgcaagata gcatcagatt cgcgtacgat ttctagcttg tcttcagtga tttcgccaag
    23881 aacacgaata ccaagaccag gaccagggaa tggctgacgc caaacgattg aatcaggcat
    23941 gcctaactga gtccctaatt cacggacttc gtctttgaat aatgtgttta atggttcgat
    24001 caattcaaat tgcatatctt ctggcagtcc acctacatta tggtgagatt tgatcgtctg
    24061 tgctgtttct gtaccagatt cgataacgtc agtatacaac gttccttgtg ctaggaaaga
    24121 gacgccttct tcaccagcaa gttttgttgc ttcgtcatca aaaacatata cgaattcatt
    24181 cccaatgatt ttacgttttt gttctgggtc agatacacct ttcaacttgt ctaagaagcg
    24241 atctcttgca tcgactttga tgatgttcaa gccgaatttt ccgcctagac tttccattac
    24301 ttgttccgct tcgcctttgc gtagcagacc gtggtcaaca aagatagatg tcaattggtc
    24361 accgatcgct ttttgtaaaa gaaccccaac gacactagaa tcaacgccac ctgaaagacc
    24421 aagcaagact tttttgtcac caacttgttc acggattttt tctacttgca tatcgataaa
    24481 gttatccatt gtccagttac cttcacagtg acaaacatca aaggcaaagt ggcgcagcaa
    24541 ttcgattccg tattcggaat gacgtacttc tgcatggaat tggataccat aaaaattacg
    24601 tgttgtgtct tggatagaag caatcggaca atctttgctt gttccgactg tttcaaagcc
    24661 ttcaggaact ttagttacca agtcaccatg gctcatccat actgtttgtt gttttggtgt
    24721 ttctttaaac aagacagctt gatcgcttgt tacttctaac aaggctttcc catattcgcg
    24781 gtttttagca ggctctacgg aaccgccaag tttgtacgtg atcaattgca taccatagca
    24841 aatccctaat acaggaatac ctaaattgaa gatctcagga tcgatcccaa atgcattctc
    24901 atcatataca ctgtttggtc cgccagaaag aatgattccc tttggattca tcgcttttac
    24961 ttcttctgca gtaatgcggt ggctcaataa ttcggaaaag actccaagat cacgaatacg
    25021 tcttgtgatc aattggttat actgactacc gtagtctaat acgataatct tttcaacgtt
    25081 cgtcatatcg gcaacgtttg tcaaatttat tacccctatc cctttaaatt ttatctcaaa
    25141 atagtcaaac tattttaaaa tgctaatatt ttcgcatgaa aatcgaatcg atgatatgat
    25201 tttcagtttt atggagaatt atatcggctc ttcctcgtgt aggcaagata tattcctgta
    25261 aatttttcaa attcacattt ttccatacat cttttgccat tttaaaggct tcttcacgat
    25321 ctccgatagc aaaacgataa taataattct ctggattttg gaaagccgta tccagtaacg
    25381 caccaaaacg atccaaatac catttttcga tcagcacggg atcggcatcg acatagacag
    25441 aaaaatcaaa aaagtcgctg acgtagattt gctgattcgc aggtaattga agcgtattga
    25501 tcccctcaac gatcaagata tcaggctgct ggatcaattc gtacttgtct ggaatgatat
    25561 catacacatc atgtgagtaa acaggtactt ttatctcttc ctgaccagat tttacacggt
    25621 tcaagaaatc gatcagcatc tccatatcat aactttcagg aaatccttta cggtccatga
    25681 ttccacgctc ttttaatata cgatttggat aaagaaatcc gtcagtcgtg atcagctgaa
    25741 cgtttcttcg tttgaaggta cgagacaaaa tcgtctgcag cagtctggct gttgtacttt
    25801 ttccaacggc tacacttccg gcgatcccga taataaaagg cggtacttct acgtatttat
    25861 gaagaaataa acctttactc aagaacaaag attcaaattc tttcatataa agataaatca
    25921 gatgagcaag aggtacatag atatctcgaa catcctgcat ggaaatgcgg tcattcaaac
    25981 ttttgatatt atctagttca tcttgtgtca aaggtgcttg cccattacga taaaattctc
    26041 gccattcttc tcttgccaca cggtagtagt tcattggatc attcatatta gcctcctgcg
    26101 ttctcccatc aatggaacta ttttaacaca aaataaacgc aaaccccacc acttaagaga
    26161 gattgccgat ctttttgtat aatctttata atagaaagaa acggagtctt attcttgact
    26221 tgcgcacgga tacataaaag gatagactgt tgtcatgaaa gaaatgagaa ggatggaaga
    26281 catgacaaat cattactatt ctgaacgccc agatacggcg cacgattttg aacaatgggc
    26341 ttttgagtta aaaggcagaa cctttcaatt tgtaacagat tctggtgtat tttcacgtga
    26401 aacagtggat tatggttcac gtgtcctgat tgatgctttc gattgggaag agttacctga
    26461 tgaaggaaag atcttagatg tgggatgcgg gtatggaccg attgggttag ctttggcatt
    26521 tgcgacacag cggtttgttg aaatggtcga cattaatgaa cgagcggtaa gtctagcgca
    26581 aggaaacgca aagcgtaacc aaatcgaaca tgttgatatc catcaatcga atatctatga
    26641 agcagttcat gaagaaacct atgcagctat cgtaagcaat ccgccgatcc gtgctggaaa
    26701 aaaagttgta catgaaattt tgacaggcgc atatcctcgg ttgaagaagg gcggtacact
    26761 gacaattgtg atccagaaga aacaaggagc gccgagtgcg caaaagaaaa tggaagaaac
    26821 atttggtaat gcagaaatcg tcacaaaaga taaaggatat tatatcatca aaagtgtgaa
    26881 agaataacta gctgaaaaat agtggagcga cgaaagagtg gaactgggcg tgcatgtacg
    26941 ggtacgtatc agttcttttt ttgttgagaa gtttttaaaa acaaagaact attttttctt
    27001 caaaaagaaa tagtaggaac agagtcgttg ttttttacga taaaataaat aagatacaga
    27061 acttgtatag aaggctgcca agtcggtaga taaaggaggt ttttatcaaa aaacatactt
    27121 tccttttttc tctattcttt tcttgggggt tttggtacac tgatagggaa tttagataga
    27181 agggagccaa taggatgaag ctgaccatca gagatatcgc agaaatggca ggcgtatcag
    27241 taacaactgt ctcacagatt ttgaataaca aaggaagcag atttagtgat aagacaagaa
    27301 aaaaagtgtt gtcgatcgtg aatgaacatc attataagcc cgattatttc gcttcaaatg
    27361 tgatcaatcg acattcaaag accatcggaa tgatcgtccc tgatgtcact gatttcttct
    27421 tctccaaact gatcgaaggt gtagagagct atttgaatcc tttgggctat gtgatcatgt
    27481 tatgcaattc tcgtcatagc caagaaaatg agatcaagta tttacaagag ctttcccacc
    27541 gctcagtgga cggaatcttg ctggccacac caaatatttt acctgagaaa tatgcattag
    27601 acagcggttt ttatcaaaaa atgccaatca ttttaatcga tcgcggattg aatcgtcggg
    27661 ataacggacg gcttatcgta aaggaatatg agggcgccta tcaagctgtt tcgctgttta
    27721 ttgaaaatgg ccatacgaag atcggcatgc tgaaggaaac cactggatac tatcaattag
    27781 aagaacgttt caacggatat cgtcatgcgc tgaaagatca tggcctgcct tttaatggga
    27841 aatttgttga acatggagag ttgactgtcc aaggtggata tactgcatca aaaaaattac
    27901 tcaaacataa agatatcacg gctatttttt gcgggaatga tgccatggcg atcggatgct
    27961 atcaggcgat cgatgaattg ggcaaaaaaa tcccagaaga tatctcagtc ataggattcg
    28021 atggattaaa gctgtcagaa tacatgattc cgcaattgac gacagtgcag cagccaagtt
    28081 ttgatattgg attttatgct gcacgtttcc tgatcgatac gatcgaattt cctcaaagaa
    28141 aagttccgaa caaagtgttc gaaacaaaat taattattcg tgagagcgta aaagtattga
    28201 caaagggcta actcggcggt atattgctta tgtaagcatt tacaaaggct agttagtgct
    28261 ttttttattt ttagtaaacc agtttactaa agtggtttac ctttcctaaa aataatgtat
    28321 aataccgaat gaagacaagg aggatcaggc aatgcgaatg gtggatctga ttgaaaaaaa
    28381 gagggatgga aacgagctgt cgaaagagga aatcgagtac atcgtcacga actatacgaa
    28441 tggaaagatt ccagattatc aagtcagtgc attattgatg gcaatttttt atcaagacat
    28501 gacaaatgaa gaaatcacga acttaacatt ggcgattgct aattctggtg atgtcatcga
    28561 tttgagttct cttgaaggaa taaaggtaga taagcattcg acaggtggcg ttggtgacac
    28621 gaccacattg attttagccc ccctagtagc tagtgtcgga gtgacagtgg ctaaaatgtc
    28681 tggacgcggt ctaggttaca caggtggtac actagacaag ctagaagcaa ttcccggatt
    28741 tcagatcgag ctttcggatg aagaatttgt acgcattgtt aatgaaagta aagtagcggt
    28801 aattggtcaa tcaggaaact tggcaccggc ggacaaaaaa ttgtatgcct tgcgtgacgt
    28861 cacagcgacc gttgattctc ttccgctgat tgccagttcg attatgagta aaaaaatcgc
    28921 ggcaggagca gatgcaatcg ttttggatgt gaccactgga gacggtgctt tcatgaagaa
    28981 catcgaagat gcaagacgtc tggcaaagac catgacgagc attgggaaac tggctaaccg
    29041 agaaacagtg gcggttatct cagatatgtc agaacctctt ggcgaagcta tcggaaatag
    29101 tctagaagtt gtcgaggcaa tcgaaacatt acaaggaaat ggtccagaag atctagtaga
    29161 aatgtgctac gccttaggaa gccaaatggt tgttcttgct ggcaaagcta agacaaccga
    29221 tgaagcaaga acgttgcttc aagaagctct ggaatcaggt aaagcgttag caaaattcaa
    29281 agaaatgatc caaaatcaag ggggagaccc agcgatcgtg gaacatccag aacgaatttt
    29341 gactgcacgc tacacaatgg agcttcctgc taaacaaagc ggagttgtgt caaaaatcgt
    29401 ggcaaatgaa ttaggaattg ctgcaatgat gctaggtgct gggcgtaaaa ccaaagaaga
    29461 agatattgat cacgctgttg gattgaaact gcataaaaaa attggtgaca ctgtgacaaa
    29521 aggtgagtca ctattgacga tctatagtaa cgacgaagaa atcacttctg tcattgaatt
    29581 attatataaa aatattgaaa ttggtgaatc ggcaatgaag ccaacgttga ttcatgacat
    29641 catcacagaa taaaggaaaa aagcggactt taccgtttta aagaaaaggg gaaaagaaat
    29701 ggaactaaac cgaatgatcg accatactat tttgaaagca gacgcatcta aagaagctgt
    29761 gatgcaaatt atcgaagaag caaaaaaata ccatttttat tctgtctgca tcaatccagc
    29821 atgggttgcg ctagcgaaag aacaattaca aggtgagcca gtagccgtat gtacagtaat
    29881 cgggtttcct ctaggagcaa acacacccga gacaaaagca tttgaaacaa cagatgccat
    29941 caataacggt gcagatgaag tcgatatggt catcaatatc ggtgctttga aatcaaagga
    30001 tgatcaacaa gtccaaaaag atatcgaagc agttgttgaa gcagcaaaag atcgtgcgct
    30061 tgtcaaagtt attattgaaa catcgttatt agatagagaa gaaatcatcc gagcttgcga
    30121 aatagcaaaa gctgcaggcg ctgattttgt caaaacatca actggtttct caacaggcgg
    30181 agcaaaggta gaggatgtcc gtttgatgcg tgaaactgta ggaccagaaa tgggtgtcaa
    30241 agcttccggc ggtatccata acgaagaaga agcaatggca atgatcgaag ctggtgctac
    30301 aagaatcgga acaagcgcag gagtagcaat cgtatctggt tcaactggtg aaggctatta
    30361 attttttcaa aggagaagct catggcagaa ctcaaacaag aatggatcaa cacagctatt
    30421 gaagcgttag ataaagccta tgttccttat tcccattttc cggtaggcgc ttgtctcgta
    30481 acggaatcag gcaagattta tcaaggcatc aacattgaga atgcatcatt tggattaacg
    30541 aattgtgccg agcgcacagc ttttttcaaa gcagtatctg aaggagaaaa aacatttacg
    30601 catctagtcg tagcggggca cacccccgat cctatctctc cttgtggtgc atgtcgacaa
    30661 gtaatggcag aattctgttc ccctgatatg ccggttacgc tagtaggaga caatggtgtg
    30721 acgaaggcaa cgaccgttcg tgcactgctg ccttatgcat ttactgaaaa agatctataa
    30781 ctcatgttca ttcagtcagc tgactgttga aaaataaaac aattatttta ggaggcttta
    30841 cagaaatgaa aaaagcaaaa ttatttggtt taggtgctgt cgcactagca gcagggttat
    30901 ttttaggtgc atgcggcaac aacggctcta ctgattcatc cggtggcaaa agctcatcag
    30961 acacaacaac tgcagcattg atcacagaca caggtggtgt agacgaccgt tcattcaacc
    31021 agtcagcttg ggaaggacta gaaaaatggg ggaaagatca aggactttca cgaggaaatg
    31081 atggcttcca atatttccaa tcttcaaatg aatcagatta catcccaaat attgaccaag
    31141 cattgaatgc tggattcaaa acgatttttg gtattggtta taaactaaaa ccagcgatcc
    31201 aagaacaagc aacaaacaat acaggtacga actttgttat tatcgatgat gtcatcgatg
    31261 gattagataa cgttgtatct gcaactttca aagacaacga agcttcttat cttgcaggtg
    31321 ttgcagcagc ttatacgact gaaacaaacg tagtcggctt tatcggcggt gtaaaaggtg
    31381 aagtaatcga tcgttttgat gctggattca aagccggtgt tgatgcaggt gcgaaagaat
    31441 taggaaaaga aatcaaagta ttgaatcaat atgctggtga cttctctgct ccagataaag
    31501 gacgttcaat tgctcaaggt atgtatgcac aaaacgcaga tatcatcttc catgcttcag
    31561 gcggtacagg taacggtgta ttccaagaag caaaatcatt gaacgaatca ggcgacaaaa
    31621 aagtttgggt tatcggtgtt gaccgcgacc aatcagacga aggcgaatac acattaaacg
    31681 gcgaaaagaa aaacttcaca ttgacatcta cactgaaagc agttggaaca gtagtagaag
    31741 atttggcaca aaaatcagcg gacggtaaat tccctggcgg cgaacacact gtttatggat
    31801 tgaaagaaga cggtgttggt ctaacagaag gacaactttc tgacgaagcg aaaaaagcag
    31861 ttgatgaagc aaaagaaaaa atcatttcag gtgacgtaaa agttccagaa actcctgaag
    31921 aaaactaaaa ccatttctat agccttgtta gacacatgtt tgacaaggct atggtttgat
    31981 tagaacaaga attgttcatt tatccaagct gccttgtgac agtttggata agggaacaac
    32041 tccctgaagt aatgtttgtt tcaaaagaag aacgctcact aagcgtgaat cttagtcaat
    32101 cgttgcccta gatagaaatc gttaggatgt gaaaaaaagg tggttcaaga aaatattgtc
    32161 atagaaatgc agaatattac caaagaattt ggaacattca aggcaaatga caacatcaat
    32221 ttacaagtaa aggcaggaga aattcatgcg ttgcttggag aaaacggtgc tggcaaatct
    32281 acattgatga acgtgctttc cggattatta gagccgacat cagggaaaat tttgatgcgt
    32341 gggaaagaag taaagatcac aagtccgacc aaagccaacc aattagggat tgggatggtc
    32401 catcagcact ttatgcttgt tgatgccttt actgtaacag aaaacatcgt gttgggaagc
    32461 gagcctagtc gcgcagggat gcttgaccat aaaaaagcac gaaaagagat ccaaaaagtt
    32521 tctgaacagt atggattgtc agtcaaccca gatgcttacg ttcgtgatat ttcagttgga
    32581 atggaacaac gggtagaaat tttaaaaaca ctttaccgag gagcagatgt actgattttt
    32641 gatgagccga cagctgtatt gacccctcaa gaaattgatg aattaatcgt gatcatgaag
    32701 gaattagtca aagaaggcaa gtcgatcatt ttgattacgc ataagttaga tgaaatcaaa
    32761 gcagtagctg accgctgtac agttatccgc cgtggaaaag gaatcggtac agtcaacgtt
    32821 aaagacgtaa cctcacaaca attagccgat atgatggttg gaagaacggt ttcattcaaa
    32881 acgatgaaaa aagaagcgaa gcctcaagaa gtcgttttgt ctattgaaaa tctagtggtg
    32941 aaagaaaatc gtggattaga agccgtgaaa aacttgaact tagaggttcg tgctggcgaa
    33001 gtacttggta tcgctggaat cgatggaaac gggcagtcgg agttgatcca agctttgact
    33061 ggtttgcgaa aggcagaaag cggacatatc aagctaaaag gggaagacat cactaataaa
    33121 aaaccccgaa aaatcactga acatggtgta ggacacgtgc cagaagaccg tcataaatac
    33181 gggttagtcc tagatatgac attatctgaa aatatcgcac tacaaaccta tcatcaaaaa
    33241 ccttacagta aaaacggcat gctgaattat tcagtaatga atgaacatgc cagagaattg
    33301 atcgaagaat atgatgttcg aacaacgaat gaacttgttc ccgcaaaagc tttatcaggc
    33361 ggaaatcagc aaaaagcaat tatcgctcgt gaagtcgacc gagatcctga tctgttgatt
    33421 gttgcaaatc caactcgtgg actggatgta ggtgcgatcg aatttattca taaacgtctg
    33481 atcgaacaaa gggacaaata caaagcagtg ttactgatca gtttcgaatt agaagaaatt
    33541 ttaaatgttt cggatcgtat tgctgttatc catgaaggag aaattgtcgg tatcgttgat
    33601 ccgaaagaaa catctgaaaa tgaattaggt ctattgatgg cgggctatac cttagaagaa
    33661 gcacgaaaag agttagaaaa gggggctggc gcacatgaat gatcgcagcg aaaaattccg
    33721 taatttatta gtgcctgtac tttcagttgt tttaggattg atcttaggcg cgatattgat
    33781 ggctgctttt ggttttcagc ccatccaagg ttattcttct atgctagatg cagcccttgg
    33841 ttcgcaaaga gggataggtg aaacgttaag gcaagcaacg ccgttgattt ttacagcatt
    33901 aggtttttct gtagctaact cagctgggtt tttcaatatt gggctttctg gacaagcact
    33961 ttgcgggtgg atcgtcagtg tctgggtggc acttgcgttt ccagatctgc caaaagttgt
    34021 tttgcttccg ttatgcgtga tcttaggtgc cttagctggt gcgatggcag cggctgttcc
    34081 aggtttactt cgagcatttt ttggaacaag tgaagtgatc gtaacaatca tgatgaacta
    34141 tatcttatta tacgtaagca catttgcttt acatgaagtg atgcctgaaa gctatcgctc
    34201 aagtttggac tcttccaata tgatcagtca aaatgcctca ctgcggttac catggttaac
    34261 acaagctttt ggcggctctc gcgtaaactc aggattattt ttagcaatca tcgcgcttat
    34321 tttcgtttgg ctgctgatga agaaaacgac tttaggattt gaaatccgtt ctgttggatt
    34381 gaatcctttt gcttctgagt atgcaggaat gagtagtaaa cgtacgatcg ttttgtcgat
    34441 ggtcatttct ggcgctttgg cagggcttgg cggtgtggtt gaaggattag gaacgtatca
    34501 aaacttcttt gttcaaacga cttcgctatc tattggattt gacggaatgg ctgtttcctt
    34561 gcttggttct ggatcagcaa tcggtattct tttatcagcg atcttgttca gtattttgaa
    34621 gatcggtgga cttggcatgc agacaggagc aggtgttccg tttgagatcg tcaatgtatc
    34681 gattgctttg attatcttct ttgtcgggat caacttcctg attcgtttcg tcatggctaa
    34741 agtcttccaa gggaaaaaac aagaagagat cgttgcgaca atagaatcga aaccaacgaa
    34801 tcaaaaccat gaagggggcg aactataatg tcgactatcg aattaattgc cttgatcctt
    34861 acttctacac tggtttattc aacaccgcta attctaacgt cactaggcgg aactttttct
    34921 gaacgaagcg ggatcgtaaa cgtagggcta gagggaatca tggtcatggg tgcttttagt
    34981 tccatcgttt tcaacttgac gatgagtcca tcacttggtt ctatgacacc atggatcgct
    35041 attttagtgg gtggcgtcgt aggtatccta ttttctctga ttcacgcagt agcaacagtc
    35101 agtttgcgtg ctgaccatat tatcagcggg acagtaatta atctgatggc acctgcttta
    35161 ggtgttttct tgatcaaagc atggtatggt aaaggtcaaa ctgataacat cacgcagaat
    35221 ctggggtatt tttctttccc aggattagca gatattccgg tcattggacc aattttcttt
    35281 aaaaatacat tcttacctgc ttattgtgct attttgattg ctgtcctagc ttgggtgatc
    35341 atcttcaaga cgaaattcgg cttgcgcctc cgttcagttg gtgaaaatcc tcaagctgcc
    35401 gacacattag gtatcaatgt atatgctatg cgttattcag gcgtactgat ttctggcttg
    35461 ttaggcggta tgggcggtgc tgtttttgct cagtcgattt ccggtaactt ttcagctggt
    35521 acgatcgttg gacaagggtt catctccatg gctgctatga tctttggtaa gtggaatcct
    35581 ttaggggcaa tgggtgcctc attgttcttt ggttttgctc aaagcttgag tatcattggc
    35641 gcacaattac cagttatttc ttcgattccg ccagtattgc tgcaaattgc tccatatttg
    35701 ctgacaatca ttgtgctggt tgttttctta gggaaagctt ctggtccaaa agcaaacggg
    35761 aagaactata tcaaatcaaa ataatctttt agcgaataaa aagggtgggt tacgggaagt
    35821 gtttttcgtc tcgccctttt tgttttaaag gcaataaaaa taaaaagaaa gaaggaataa
    35881 tcatgtttaa acgcgtacat ctgatcgtca tggattcagt aggaattgga gaagcaccag
    35941 atgccgataa gttcggtgat gttggttctg atacattagg acacattgcg aaagaagcag
    36001 gattgaacgt tccacattta gagcagcttg gtttaggaac gatccgtcct cttgaaggtg
    36061 tggaatcagt gaaagatcat caaggctatg cgacgaaatt agaagaagtt tctgtaggga
    36121 aagatacgat gacaggccac tgggaaatca tgggattgaa tatcaaaact ccatttcgtg
    36181 tgtttcctaa tggattccct gatgatctga tcaaacaaat cgaagaattt tctggccgta
    36241 aagtcgtatg taacaaacct tatagcggaa cagctgttat tgacgattac ggtgaacacc
    36301 aaatgaaaac aggcgattta attgtatata cttctgccga tcctgtcctt cagatcgcgg
    36361 cacatgaaga aatcattcct ttagaagaat tatatcggat ttgccaattc gttcgtgata
    36421 taacaaaaga tgatccgtat atgattggcc gaatcattgc acgtccgtac gtgggtgaac
    36481 caggagactt cacacgaaca agcaatcgcc atgattatgc gttgaatcca ttcggacaca
    36541 cagtccttga ttctttgaaa gaagcaggaa aggacgtgat tgctgttgga aagatcaacg
    36601 atattttcaa cggacaagga atcactgaat ctgtccgcac gaaaagcaat atggatggcg
    36661 ttgaccagct gctgaaagtt atgaaaaacg atttttcagg aatcagcttt accaatttag
    36721 tagattttga tgcattatac ggtcatcgtc gggatgtagt cggctatgcg catgcgattg
    36781 aagaatttga cttacgaatt ccagaattgc ttgaagcgat ggaagaagac gacttgctaa
    36841 tgattacagc cgatcacgga aacgatccga cgtttactgg taccgatcat acgcgagaat
    36901 atgtaccgtt attggtttac agtaaaaaaa tgaaagaaca aggggcattg cctcaaggat
    36961 attattcaga tatttcagca acgattgctg agaactttag tgtttcttcg actgaaaatg
    37021 gcgaaagctt cttgaaatta ttaaaataat ctttttaaat aggagaaagc aaatgaccaa
    37081 gttaagcgag atgttacaag aaacgaccca gtttatcctt gataaaggag taaaagaaat
    37141 tgattttggt ttgattctag gttcaggatt aggtgaatta gcagatgaaa tcgaagatgc
    37201 tgtaatgatt tcttatgatg agatcccgtt ctttcctgtt tcgacagtag ttgggcatgc
    37261 aggaaagcta gtttatgggt ctctggcagg caagaaagtg ttagccatgc aagggagatt
    37321 ccactactat gaaggtcatt ccatgcaaac agtgacctat ccagtacgtg tgatggccgg
    37381 actaggggcc cattcagtca ttgtgactaa tgcatgtggt ggtgtcaacg aatcatttgt
    37441 accaggagat ttgatgctga ttaccgatca tatcaatttt actggtgaaa atcctttgat
    37501 cggtccgaat gaagatgcaa tgggccctag atttccagat atgagtcaag cttatacgcc
    37561 tgactatcaa gcaacagcaa aagcagcagc agaaaaacta ggtttggatc ttaaagaagg
    37621 cgtatatatg ggctattctg gaccaactta tgaaacgcct gcggagatcc gaatgtctcg
    37681 gacaatagga gccgatgcag tagggatgtc gactgttcca gaagtcatcg tagcagctca
    37741 cagcggatta aaagtgttag gaatctcttg tattaccaac ttagcagcag gtatgcaagc
    37801 gaatctgagc catgaagaag ttgtagaaac aactcagaga gtcaaacaat cttttaaagc
    37861 attggttaaa gaaacattga cattattgta ataggaggaa caataaaatg agtgtgcata
    37921 ttgaagcgaa agaaggcgag atcgccgata agattttact tccgggagat cctttgcgag
    37981 ctaaatatat tgcggagact tttttggaag atccagtttg ttacaatcag gtacgcggta
    38041 tgttaggctt taccggcaaa tataaaggag aacgtatttc tgttcaagga acaggaatgg
    38101 gaatgccatc agccaccatc tatgcccatg aattgatcca gtcgtatggt gtaaagaaat
    38161 tgattcgagt aggtacatgc ggtgcattgt caaaagacgt ccatgttcgt gatttagtac
    38221 tagcacaagg tgcagcgacc agttcttcga tgatcgaaaa aaatttccaa gcctttcatt
    38281 ttcctccaat cagtgatttc aatctattac tgaaagcata tgagatagct aaagaaaagg
    38341 gctatacggt tcatgtagga aatgttcttt cggaagattc tttctacaaa gatgatttga
    38401 cagagacgtt ccaattagct gaacttggcg ttttaggagt ggaaatggaa gcagcggctc
    38461 tttattactt aggcgcgaaa taccatgtgc agacattaag tttgatgacc gttagcgacc
    38521 atttgatcac cggtgaagaa acaacagctg cagaacgtca atctacgttc aatgaaatga
    38581 tcgaagtagg attggaaaca gcgatagcag aataagaagg aaatgaaaaa gctgccctgc
    38641 attaagtaat aagaacatcc aagcaaaaat agctcctcat gtttttgact tggctggact
    38701 tattattgca aatgtaagct tttgaacacc gtttaccaag aggttgtgaa tctgccgttt
    38761 aactctgagt cacaaggaac aatttcaaaa a
//
LOCUS       DARBFO010000263         9983 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.guided.093, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000263 DARBFO010000000
VERSION     DARBFO010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.guided.093"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..579
                     /locus_tag="SCK51_003103"
     CDS             <1..579
                     /locus_tag="SCK51_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEG1253630.1"
                     /translation="FLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERA
                     ESYKQEFIKRDVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTL
                     RTFESMYFQKKLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLE
                     FYDAENWAKRFITNDSTIYEQYEYDLSSVENK"
     gene            complement(729..1670)
                     /locus_tag="SCK51_003104"
     CDS             complement(729..1670)
                     /locus_tag="SCK51_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG1253631.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1796..2917
                     /locus_tag="SCK51_003105"
     CDS             1796..2917
                     /locus_tag="SCK51_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG1253632.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3175..4677
                     /gene="eat(A)"
                     /locus_tag="SCK51_003106"
     CDS             3175..4677
                     /gene="eat(A)"
                     /locus_tag="SCK51_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEG1253633.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4705..5271
                     /locus_tag="SCK51_003107"
     CDS             4705..5271
                     /locus_tag="SCK51_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1253634.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            5482..6543
                     /locus_tag="SCK51_003108"
     CDS             5482..6543
                     /locus_tag="SCK51_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEG1253635.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6546..7442
                     /gene="murQ"
                     /locus_tag="SCK51_003109"
     CDS             6546..7442
                     /gene="murQ"
                     /locus_tag="SCK51_003109"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEG1253636.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7456..9438
                     /locus_tag="SCK51_003110"
     CDS             7456..9438
                     /locus_tag="SCK51_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1253637.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9503..9949)
                     /gene="nrdI"
                     /locus_tag="SCK51_003111"
     CDS             complement(9503..9949)
                     /gene="nrdI"
                     /locus_tag="SCK51_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEG1253638.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3258 a         1777 c         2168 g         2780 t
ORIGIN      
        1 tttttggaag caaaagaact tgggctgaaa cataatagtt cattttattc gaaacatatg
       61 aatttttctt ctagcgaacc aaagacggat ctattttttg gtgcaacgaa taatgggaga
      121 aaagaacgag cagaaagcta taagcaggaa tttataaaaa gagatgttac gaccaactat
      181 ttcattcttc cacatcgagg gaatgaacaa cctggatatc ttacttatga agagtacctg
      241 aaaaaaacag gggaatcgcg cgggattctg gaactgttgc gggagggtca gcaaggagtg
      301 actttgcgga catttgaatc catgtatttt caaaagaaac tagtgacgga taatcaagcg
      361 attgctcatt atcattttta tcgtcctgaa aatatttttt tgcttcgaga acgtgattta
      421 gatgaattac cggaatttct gcatacacca tttaagccaa tcctatcaga gctattggaa
      481 ttttatgatg cagaaaattg ggcaaaacga tttatcacta acgattccac tatttatgag
      541 caatacgaat atgacctctc ctctgttgaa aacaaataaa aaacctacat tttgaagaga
      601 ttatgtactg aaacaaacaa gcacaattct tatggcatca tgagagttgt gttttctttt
      661 tgatctttta ttgggcttaa aaaaagagaa ccagctgctg attcagctga ttctcatcag
      721 agagcctttt attctgcgcc aaggattcct tcttttgcat ggactaattg ggtcaaaaag
      781 gcacaatatg gtgtagcaat atcatatttt tgtccttttc gccaaatggc accattgatg
      841 tagtcgattt cagtcaaacg atggtttttg atcaggtctt ggtacataga tgggtaatgc
      901 aacccgatgt tgtcaggatc ataagtagaa gcaatatgtt ggtacacttc ttcttgatct
      961 agtatgattc cttcttttgc tgcgatagaa gcaaattcag atacgattgt tcgaaccatg
     1021 ctctctgccg ctttttgcgc gcctagttca gcaatattac aatccaaaat cgtgcataaa
     1081 ccattcaatg ttccatttac acaagctttt cgccaaatgg aataacgaac gttatggcta
     1141 tagatcggat ttaaatttgc ttcttgaaag acttctacaa ctttttttgt aaatgcttcg
     1201 ccttctggtt ccaagttctc tagttcgatt tctccatcac ctaacaatgt gacttttccg
     1261 ggaccagcta gaccagctgt ccacatcgtg atcccaaata agatattttt ctttggcaca
     1321 tatttttcca gtacatcttc atgacctaat ccatttagta aacacaaaac ataagtattg
     1381 tcagtaataa tggattggat cgaacaaaac atatcatcta attgattggc ttttgttaat
     1441 gctacgatca agtccacgtg ttcgttgctt tcgatgattt cttctggtga atagattggc
     1501 agttttgcga ccacttcttt accgttaaag tcagcaatca acccatttgt acgaatggct
     1561 tcaatgtgag caggccaacg atcgatcaag aggacctcat ttccgctttg atgtagcatc
     1621 aatccgattc ggctgcccat tgctccggcc ccagctattg cgattttcat gttttttctc
     1681 tcctttattc aatgagttcc cttttagtat atagaaaaat ggaagaaaat agcattgttt
     1741 tgtttgaaaa aaatgaaaaa aagtgtctta cttaagaaga aagtgaggga tacagatgga
     1801 tattttggcg gcaattgatt catttaaagg aagtgcttct tcatttgaac taaatcaggc
     1861 agttctaaat ggctgggaag atcgtaaagg aagaaaaatc aatgtgccga ttgcagatgg
     1921 cggagaaaat actgcagaag caatctatta cgcgctcagg ggaacatggc gtaccattga
     1981 tgggatggat ctcttattcc gtcagcgaaa aatcagctat ttattgacaa cttggcaagg
     2041 aaaaaacacc gcagtcatcg aatctgcaca agcattgggg ttagatctac ttgataaacc
     2101 aacggatcaa actatccgac ttgcttccag ttatgcctta ggagaactta ttaaagatgc
     2161 actgcttcaa gaagtagaac aaattattgt cactttagga ggaagcgggt gtagcgatgg
     2221 tggtctagga ttactccaat ccttgggtgc ttctttagaa gggtacacag aaggaaatcc
     2281 tttattatct actaaaaaac ttcattttga tcgaagaaac gttcttgttt ctaaagtaaa
     2341 attgattatc gctgcagatg ttaccaatcc atatacaggt caacaaggag ctgcttggca
     2401 atttggaaga caaaaaggag gaatagatac gacgctagct ttcttagata aacaagcagg
     2461 aaagattgct cagcaaataa aagatttctg ggaggtggat ttagatgata ttccaggaag
     2521 tggagcagca ggcggtattg gtggagccct ttttttgttg ggagcgaaga tggtttctgg
     2581 tttcgattta gtcagccaaa tcactggatt ggaaaaaaag atcgaacaag cagatgtcat
     2641 cattacagga gaagggcgga tcgatcagca aacgatccac ggaaaagttc catacggtgt
     2701 agcagtgctt gcaaaaaaat atcgaaaacc aataatcgct atttgcggaa gtagagacca
     2761 agagctagaa ggactttctg attttctccc aattgtctta agtattcatt tgggtccaat
     2821 aggtttagaa gaagcaatgg aacacaggca aacattagaa aaaacaaaaa tcttaggaca
     2881 gacacttagc agatttttca ccatcctccc gaagtagttg acaaaatcat tttctttatg
     2941 ctatcgtaat ttcaaatata ggacaaagtg agggatccgt gcattgaaaa attaagtcga
     3001 ttgatgaaag ctgcattcag actgtacctt gtggagacaa ggaagcagtg tgcagatttt
     3061 cgggattggc ttggtttttt cgatgtatgg gtctttttgt gtgtatccaa cccttgcatc
     3121 ctctttagcc agttctgaaa ataggactgg tcttttttta cgaggtgatg gaaaatgtct
     3181 aaaatcgaaa taaaaaatct gacattcggc tacgacagcc aaggcacatt attatttgaa
     3241 caagcaaatc taaattttga cacacaatgg aaactaggac ttatcggacg aaacggtcga
     3301 ggaaagacaa ctttactgaa tattctacaa aacaaactac cttatcaagg gcaagtaatc
     3361 catcagcaag aatttgccta tttcccgcaa cagacaaaag ataaagaacg tttaacctat
     3421 tacgtgttaa atgatattac ggattttgag atatgggaaa tcgaaagaga gctccaattg
     3481 atgcaaacag atcctgaaat cttatggaga gaattcagca cactatcggg gggagagaag
     3541 acaaaagtcc tactggcact tttatttgtg gatgacactc atttcccgtt aatcgatgaa
     3601 ccaacgaatc atttggatat ctctggtaga aaacaagtag cggcttattt gaaaaagaaa
     3661 aaacaaggct tcatcgtggt cagccatgac cggggattta tcgatgaagt agtggaccat
     3721 gttttagcaa tcgaaaaaag tcaactggaa ctttatcaag ggaatttctc tatctatgaa
     3781 gaacagaaaa aacttcgtga tgaatttgaa atggctcaaa atgaaaaatt gaaaaaagaa
     3841 gtcagtaggc taaagaaaac agcagctgaa aaagccgaat ggtctcgttc ccgagaagga
     3901 gataaaacaa agaaacaagt cggattcatc gatactgaat ctagacgagt gaataaagga
     3961 gcagtgggtg ctgatgctgc acggacgatg aaacgatcca aagcaatcgt gaatcggatg
     4021 gagacccaga tcagcgagaa agaaaaacta ttaaaagata tcgaatatat cgattctttg
     4081 acgatgaata gccaagcgtc tcaccataag cgacttttaa gcgtagaaga tcttcaatta
     4141 gggtatgaaa atctgttatt cgagccaatt cattttacaa tcgagcctca tcagcgggtg
     4201 gcgatttcag gtcctaacgg tgcaggaaag tcatccatta tccattatct tctgggggca
     4261 ttcaacggca aggttatagg agaaaaatac cagccaaaac atctgagcat tagttatgca
     4321 agccaaaatt atgaagacaa tcgaggaacg ttggcggaat ttgcagagaa aaaccaagta
     4381 gactaccaag catttttgaa caacctccga aagcttggga tggaaagaga tgtttttcat
     4441 aacaagatcg agcagatgag tatgggccaa cggaaaaaag tggaattggc taaatcttta
     4501 tcacagccag ctgaactata tacatgggat gaaccattga attatttgga tgtcttcaat
     4561 caagaacaat tagaacaact gatcttgaac gtgaaacctg ccatgttact agtggaacat
     4621 gatcaaacct tcctggataa agtatctact gagattattt ctcttgagag aatctaaccc
     4681 ttgtaatctc aagaacaaca aggtatggtt aaggggaact ttgccaacga aggagagaat
     4741 cacatgcttt atgtaacgag acacggagaa acaacttgga acgcacaagg attagtttgc
     4801 ggacgggcag atgtgccgct gactgaaaaa ggacagatgc aggcacagaa actagcagaa
     4861 aaggtagtag atttgcctgt tccaatcacg aaaatcatcc attcccctct tcaacgagca
     4921 agagatacag cacaagcagt tgctgatcgt ctcagcttgc cgctgaccgt tgatgaacga
     4981 ttggtagaaa tggattttgg ggattatgat ggactgccaa gcaaagatga gaactttcaa
     5041 aaagcacgtt tagcgttcgc tgtacgattc ccaaatgggg agtcagtctt agatgtgtat
     5101 gcacggatcg ttcctttatt gaaagaatgc atcgaagacg aagaaaatgt ctatctgtta
     5161 gtttgccata atgccttgat aagagtgatc aatgcttatt ttcatccgat gcctaatgag
     5221 gggttcttta cattcatggt agataataca gaattggttt cttatgagta attatgtttt
     5281 tgtgttattt aaataaaata tttcaaggca ctgcttttgc ttagcagtgc cttttttagg
     5341 ttatgacaat tcttttgata atataaactc aacctttaga aatgaaattt aattatttta
     5401 ataaaagtat tgaaattttg tttctttaga tatatactct agtcatagaa atgaagacgg
     5461 atacagaaaa agagggataa tatgtttggc ttttcaatat ttatgaacga ttctttgtca
     5521 gaagagaaga aacagtatat ccgccaaatg gcatccaatg gctttgtcgg tattttcact
     5581 tccatgcata taccagaaga tgatgtttcg gcatatcaaa agcgattgat cgaactagga
     5641 agcgaagcga aaaagaataa actagaattg atggtcgata tttcaggaga ggcattggaa
     5701 cgagcgggtt tttcattaga agatctaacg cctttgaaag caattggtgt aacaggttta
     5761 agaatggatt atcatatttc caatcagcag attgccgact ggtcacatca attgaaaatc
     5821 agtttgaatg ctagcacgat cacgcctaaa gatatcgatg aattgaaaga agcagaagct
     5881 gatttctcac agattgaagc ttggcataat tattatccac gtcccgaaac agggctggac
     5941 aaagaatggt atcagaaaaa aaatcaatgg ctcaaaattc aaggtctcct tgtccaaggt
     6001 tttgtaccag gagatacaga gttgagaggc ccactatatc aaggattgcc aactttggaa
     6061 gaacatcgag ggatccatcc tcttgcaggc gctctagatc tattagcaag taacacggat
     6121 attgtttaca tcggagatgc aggtctgtca gaaaacgttc aggagcaatt tgcttctttc
     6181 caaaaagaac agacagtctt gctccatacg gaacctgtag atgaagaatt ttacgaatat
     6241 atattaggga agcatacgaa tcggcaagac gatgcaagag atgtcatacg aagcgtagat
     6301 gcacgttttc gagagattcc gcctatccca gcaagaaata cagctacgcg tatgaaaggg
     6361 tctattactt tagataacga aaagtatttg cgctatatgg gtgagatcca attaaccaaa
     6421 tatgatttgc cagcggatga aaaagtgaat gtagtagcta aagttatcaa agaagagcta
     6481 ccattgatca accaaatcaa agctggtatg aactatcaat ttataagaaa agaaggaaga
     6541 taaaaatgag caaaatcaat ttagagaacc taacaacgga acgcagaaac caagaaactt
     6601 ttggattaga tgagatgagt atttctcaag ccgttcaact aatgaacaaa gaagatcaga
     6661 aagtggccgc tgcggttgaa aaagaactga caagtatcga accagtgatt gctaacacga
     6721 ttgagtcctt taaaaaaggt ggacgtctga tttatatggg agccggaaca agtggaagac
     6781 taggtgtgtt agatgctgct gaatgcgtac cgacattcgg tgtagaaccg gaaatggtca
     6841 taggtctaat tgctggtgga gaagaagcaa tgactgtagc tgtcgaaggg gccgaagatt
     6901 cttatgagct agggcaagaa gatctaatca agctgaattt gacaaaaaat gatatagtta
     6961 ttggtatcgc agctagcggt cgtacaccat atgtgatcgg tggattagat tacgcaaaat
     7021 cgatcggtgc tgtcacagga acgatttctt gcaataaaca agccgaaatc agtaaacacg
     7081 cggatttccc aatcgaagtc gatgcaggtc cagaattttt gacaggctct actcgattga
     7141 agtcaggaac agcacaaaaa cttatcttga atatgatttc tactatcagt atgattggta
     7201 taggtaaagt attcaataac ttgatggtcg atgtcaaacc aacaaatgaa aaactaatcg
     7261 aacgctcaaa acgaatcatc atgcaagcga cagaagctga ttacgaaaca gctgaaaagt
     7321 actttgaaga agcagatcaa aatgttaagc tggctatcgt catgattttg acaaatagtg
     7381 ataaagagga agctcaagca aaattagctg aagcaaacgg atttgtaaaa gggaccttat
     7441 gagggggaaa agaccatggc aaaaaacgaa ctgactttgg aagaactggc aaaacaaatt
     7501 tataaagggg caggcggcat gggcaatgta gaaagcattg tccactgcat gactcgtgtg
     7561 cgtatgaaga tcatcaatga tgatcttgta aatgtctctg aattaaaagc gattccagga
     7621 gtgcttggtg tagtagaaga tgaacagctt caagtcatca tcggacctgg gaaagtgaac
     7681 aaagtagcac agcaaatggt tgatttggca ggcgtgaaat taggagaaaa actttcttct
     7741 gtaagtacag ctacagcaaa ttcatcagca tctggaaaag accgtgtaaa cgaacgggca
     7801 caagaaatga aagcggcaca aaaatcgaaa caaaaaccat ctaaaatcaa atcggtatta
     7861 aaagacattt ctaatatctt tgtgccaatg attcctgcat ttgtcggtac tgggatcgtg
     7921 gcagggattg ccgcagtttt agcaaactta gtcacagccg gaacacttga tgcagcaaca
     7981 tggcaacaat atatcgatgt gatgaacatc ttgaaaaacg ggatgtttag ctacctagtt
     8041 atctatacag gtatcaatgc tgcacaagtt tttggtgcaa caccaactct tggtggagtt
     8101 atcggggccg ttgtgatgct gacagggatg aatccggaag caccactgaa aaacttattt
     8161 actggtgaag ctcttgcagc tggacaaggt gggatcctag gtgtcatctt cgctgtttgg
     8221 ctattgtcaa tcgttgagaa aaaactgcat aaagtagtac ctgatgcggt agatatcatc
     8281 gttacaccaa ctctttcatt gattgccatc ggtctgattg aaattttctt catcatgcct
     8341 ttagcaggtt tcatttctga tggcatggtc ggtgggatca actgggtact tggcgttggt
     8401 ggagcatttg ccggcttcgt tctaggcaca ttattcttgc cgatggttat gttcggactg
     8461 catcaaattt tgactccaat ccatgtgcaa atgattgatg aaactggtcg tacgctattg
     8521 cttccaattc ttgcgatggc tggtgcagga caagtgggtg cagcattggc attatggatt
     8581 cgctgcaaaa aagataaaga attaacagaa atgatcaaag gagctttacc agtcgggatc
     8641 ctaggaatcg gcgaaccatt gatttacgga gtaacattac ctctaggacg cccatttatt
     8701 actgcttgta tcggtggagg tatcggcggg gcagtgatcg gaatgatcgg aaatgtcggg
     8761 gctatcgcta ttggaccttc aggagccgct ttgatcccat tgatctctga cggtaaatgg
     8821 ttaggctaca tccttggatt attggcagct tatgctggtg gcttcgttgc tacattcttc
     8881 ttcggtattc ctaaagaccg tttagaaaaa gaagaattag cgactgaaac agccacagta
     8941 aatgaagtgc ctgaaaaagt agtacctcaa acttcagctg aaagtgtgac tatcctctca
     9001 tcagttgctg acggaacagt tgtttcttta gataaagcaa gtgatccagt ttttgcacaa
     9061 aagatgatgg gtgaaggcta ctttgtagaa ccagaaaacg gacatatcta ttctccagtt
     9121 actggggtag tcagctctgt cttcccaacg aaacacgcta ttgggatcac aacgccaaat
     9181 ggtttagaaa tcttgcttca tatgggaatc aacacagttg acttaggcgg tacgccattt
     9241 gatgtgaaag taacagaagg gcagcaagtg acacctgata cgcttgtagc agaagctgac
     9301 ttagaagcga tcaaagcagc aggcaaagaa acatccatga tcgtcttggt aaccaatatg
     9361 gatcgtgtaa aaaactttgt cttggaaaaa acaggtaaag caaaagcaaa agcacctgtg
     9421 atggatgtca aagcttaata tgatagaaac aatagaaacg aaagaagaaa ctgaaatcta
     9481 tttttcagct tcttcttttt tattatttat tgttttctgc taaaatctga taaattcgtg
     9541 tcacatcaga tggtgttccc cgtaattcat aatctgccaa gaaaggaaag ccaaatgttt
     9601 gtgcatattg tttggctgtt agacaatact gattattgaa gtttttattt ccgctaccga
     9661 ctacaccaag acaaagttta tgattgtctt catgttccag atattcacgc atcgtttcgg
     9721 tcaagatttc tgtatcaccg ttatctaaac catttccgcc gtctaaataa gtaggaacga
     9781 aagtaaaaaa tggttctgtt tctttcgtaa aatcactatt ttcatggatt tcttttaaag
     9841 taacttcagg attgacagga tcttctgcat gcattttttc agcatattct gctagatgtt
     9901 tggcaaaggc acgcgtattt cctgaaatcg atatatataa aatgttcatg gatcatcctc
     9961 caattctttc cttttagtat agc
//
LOCUS       DARBFO010000264         8894 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.guided.103, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000264 DARBFO010000000
VERSION     DARBFO010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.guided.103"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            54..623
                     /locus_tag="SCK51_003112"
     CDS             54..623
                     /locus_tag="SCK51_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEG1253639.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            690..1427
                     /locus_tag="SCK51_003113"
     CDS             690..1427
                     /locus_tag="SCK51_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1253640.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            1658..2926
                     /locus_tag="SCK51_003114"
     CDS             1658..2926
                     /locus_tag="SCK51_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG1253641.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            3413..3877
                     /locus_tag="SCK51_003115"
     CDS             3413..3877
                     /locus_tag="SCK51_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1253642.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            3861..4490
                     /locus_tag="SCK51_003116"
     CDS             3861..4490
                     /locus_tag="SCK51_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG1253643.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            4844..6322
                     /gene="msr(C)"
                     /locus_tag="SCK51_003117"
     CDS             4844..6322
                     /gene="msr(C)"
                     /locus_tag="SCK51_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEG1253644.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            6369..6815
                     /locus_tag="SCK51_003118"
     CDS             6369..6815
                     /locus_tag="SCK51_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEG1253645.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(7014..7529)
                     /locus_tag="SCK51_003119"
     CDS             complement(7014..7529)
                     /locus_tag="SCK51_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1253646.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            7646..8848
                     /locus_tag="SCK51_003120"
     CDS             7646..8848
                     /locus_tag="SCK51_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1253647.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         2862 a         1553 c         1860 g         2619 t
ORIGIN      
        1 attagacatc ataagccgtc cgttataaag taaggataaa aggagggagc aaaatgacaa
       61 gcctcatcga aaaacaaatc gtcaataaag agattcgagt aacggatccg ctctttgaag
      121 tgatccatca gatccaagcg gaaaacgaag agcctttagc agcgctgaat actgggtacc
      181 atgaaccagc agatatccgc aaacggctag aaaatatcat tagtgacaaa gtagatgata
      241 cggtgactgt tctgctgcct ttttatacag atttcggcaa acatattagt atagggaaaa
      301 atgtctttat caatcgtcaa gtgatgttcg ttgacttagg tggcatctgt ttagaagatt
      361 cagtattgat tggtcctcgt gtcaacctca tcacggtgaa ccatctaacc gatccaaaag
      421 aacgaagagg cttgtcagta aagccaatcc acatcaagaa aaatgcttgg atcggagcag
      481 gagcaactat tctgccaggc gtaacgattg gcgagaatgc cattgttgca gcgaatgcaa
      541 ctgttacgaa agatgtccca gacaatacca tcgttgcagg aatccctgcc aaaatcgtca
      601 aacctgtcga acggattatt tgaacagtaa gatgaaataa gagatagtga gtataagcaa
      661 gagatttttc aaaacaggag gagaatagaa tgaatgattt atcagaaaaa gtgattgtca
      721 tcatgggcgc ttcaagcgga atcggtgaag caacaacgaa gctattagca gaaaaagggg
      781 ctaaactagt tatagctgca cgccgagaag atcgcctaaa agcaataaaa gaatctttac
      841 cagaagctga gctatatatt caaacagcag atgtaagaga ttttgcacaa gtccaagcag
      901 ttatcgattt agcgatggaa aagttcggtc gaatcgatgt attgtacaat aatgcaggaa
      961 tcatgccaac cgctccatta gttgaaggtc accgtgatga atggcaaaat atgcttgata
     1021 tcaacatcat gggtgtgtta aatggaattt ccgccgtact tccaatcatg gaaaaacaaa
     1081 aatctgggca tatcatttca actgattctg tggcgggaca tgttgtttat cctgattccg
     1141 ccgtttattg cggaacaaaa tttgctgtcc gtgcaatcat ggaaggatta cgtcaagagc
     1201 aacggcaaaa taacatcaaa tctacgatca tctctccagg agctgttcaa actgaattat
     1261 atcaaaccat cagcgacaaa aaggctgctc ttgaacttca tgaagcgcaa aaagaatggg
     1321 gattaacatc tgaagatata gcttctgctg tagcatttgc gatagaaacg ccagaccgga
     1381 tgtctgtcag caacatgatt atccgtccga cgacacagga agtataatcc ataacggata
     1441 atatccagag agaattactt gttaaatagt tattcaaacg accatcaatt taaggcgtga
     1501 atggagaaga atattcttca tttacgtctt tttatgcaga aaaagcaaat ttattaaaaa
     1561 gaaacaaatg ttatttaaat atggacattg acttgctttt acttttttag ttacactgtt
     1621 atttaggaaa agtttcataa ttcaaaggag aaaaaagatg aaagcaaaga atttattatt
     1681 tagcgttatt cttttaggaa atctagcagt tgccaacaca acagtgctag ctgaagaatc
     1741 gacagaaact gtcgcaactc agacatcgag ttcttcagaa acaggaattg aaaacgatac
     1801 agaaagtact ttgcagtcag ctactgtctc agaaaataca atttcaaccg aagaaccaac
     1861 aactgaaaca acagaacaaa cacaaaccga agacagtaca tcagcagaag ctcagaacaa
     1921 tccagagaat gcagaagaaa tcacgaacca attgacagat gggcaaacat tgacacatac
     1981 ccatcaagca gatttgtctg aattcgaaaa ctatactggc tatgcttttt atgctcgctc
     2041 atctgctaat tctcaacaag cgttcattga tagtattgcc tcaactgcac aaagtttggc
     2101 aagtgccaat gacttatatg catcagtcat gattgctcaa gccatcgttg aaagtggctg
     2161 ggggaacagt actttggctt ctgcccctaa ttacaatcta tttgggatta aaggaagcta
     2221 taatggccaa tctgttacga tgcctacttc ggaatatgtg aatgggcaat ggattacggt
     2281 gaatgccgct tttagaaaat atccttcgta caaagaatca ttgcaagata acgtaacggt
     2341 attgaagacg acttcttttc agccaggcgt ttactactat tccggtgcat ggaaaagcaa
     2401 tacgaactct tacaaagatg ctacggcttg gctaacaggt cgttatgcca ctgcgcctaa
     2461 ttatggttct acattgaaca atgtaatcga aacctataat ctgacacagt atgatacagc
     2521 accaacttca acttctccga tgtatcgtct gtataaccgt catacaggag aacatttata
     2581 tacattgaat gccggagaaa aaaattatct gccaactgta ggctgggaat atgaagggat
     2641 tgcttggcaa gcaccaaaca gcggacaacc tgtctaccgt ttatacaatc cttacagtgg
     2701 agaccatcat tacacaatgg cacaatctga gatcaactct ttaacaaaaa tcggttggcg
     2761 ttatgaaggt ctaagttttt attctggtgg ttccaagcca atttatcgac tatttaatcc
     2821 aaatgaaaaa actggtaccc atcactatac cttgagtgct aaggaaagag attggctaac
     2881 accgatgggc tggagatatg aaggaatagg attttatggt tattaaaaag aggtagtgaa
     2941 aaagctgccc tgcatcaagt agtaagaaca gccaaacaaa aacagatttt catgtttttt
     3001 gtttggttga acttattacc gcagatgcaa gtttttgaac accgtttact caaaaaaggt
     3061 tgtgacaaca tcttgtcata acttttttat tactgtttaa gcatactgat ccaatttcta
     3121 atattcgacg ggattcttgc aaaaggcgat ttcggaaatt tttggcggtg taaaattatc
     3181 tactgcagaa gagctatctt ctatagtttg ataagaatca ataatcttgg ttttagcgtt
     3241 gtgctttctt tatcattttt tccgttcatt ctacaaaaaa tttcttttct ctatactttt
     3301 ctattgagag cgtttttttt attacacagc tgattctttg acttttttct ttttttattc
     3361 tattattgac aagtcaatcg ttgacgagtc aattattaga aaggaggaaa ttatgtcttt
     3421 agaagagatt agtcaccttt tatatcaaat caaattagcc gatcaaaaaa tcgtccagat
     3481 ctttgaagaa aatatcggta taagtcttac tagatacgag atcatgatga gactgaaaga
     3541 agaaaatcca ttgctacaaa gcagtttgca agaaaagttg cagattgatc aaggagcagt
     3601 tactcgtcat ctgaaggtat tggaagcaaa tggctatgtt tcgcgtcaga gaaatcctca
     3661 gaacaaccgg gaagtgtatg tagcgctaac agaaaaagcc cagaagaaaa tcaagcattg
     3721 tgaaatggac cataaagata caggttccgt gctatccccg gctttcagcg atgtagaaat
     3781 gaaacaattt ctaacacttt tagataaatt gaatacccaa ctttgcaata taacaacaga
     3841 aacgaaagag gttaataata atggaacaaa aaaatgattt ttcaacaatt atgactggta
     3901 gaagatccgt tcgcgtatat gatgaaacat acaaaatccc acacgaagaa atgctggaaa
     3961 tgatcaaaga agcgacgact gcaccttctt ctgtcaatat gaaaccatgg cgctttgtgg
     4021 tcgctgaaag cgaggaagca aaagaaatct tgcgtccttt gatccgtttc aatacgagac
     4081 aaaatgatac ttcttctgca atggtcatga tttttggcga tatgcaatgt tacgaatatg
     4141 gcgaagaaat ctacaaccaa gcttacgaat cagggaagat gccaaaagaa gtcagagatc
     4201 aacaattagc tgcgatcatt ccttattaca agagtttttc aagagaagaa atgaacgatg
     4261 tagtcaaagt cgattctagc ttagcggcta tgcaattcat gttagttgcg cgtgatcatg
     4321 gctatgaaac aaatccaatt ggtggattcg aagcagatca gttggcagag gcatttggtt
     4381 tggataaaga ccgttatgtt ccagttatta ttttatcaat aggaaaagcg gtagaagaag
     4441 gttacgagtc tgttcgttta gcaccagaaa agatcactat gttcgaataa aaggttttca
     4501 gttactattc agtagctatt gctttttttg aatcgtgtgt ggtaacctaa taaaaaagga
     4561 ggtcggaaaa atgactgcat cgatgaaatt acgtttcgaa cttttgaata acaactaaat
     4621 aaattcaaaa gacatgtata aacaacaatt tcatggatgg agtatgtcca ttttttcgaa
     4681 cgacagtaga ataggtgaaa gctatgacag atttggtgat ttagtcatag ttatttcgtt
     4741 cagagtagga aaagatgtga tgcagttttt ttgcaaacag atttttctgc tcttttttag
     4801 tatcgataat tgttgtttta catgaaatag gagtgtaaaa catatggaaa atttagcagt
     4861 aaatataaca aatctacaag tcagctttgg caaccagcta gaactatcta ttgattctct
     4921 tcgtgtctat cagcaagatc gtatagggat cataggagaa aatggtgtcg gtaaatcgac
     4981 tttgctcaaa ctaatagccg gtgaactttt tcccgatcat gggaaaatcc aaacagagat
     5041 caccttcaac tacctgcctc aattaaccta tctcgctgaa gcaaaggacc taaatttgga
     5101 attagccagt catttccagt taagactgga agaaacttca gagcggaaat ggagcggagg
     5161 ggaagaacga aagatcgagt tgatacgtct tctttcttct tatgaacaag ggatgcttct
     5221 agacgagcca acaacccatc tagatagaaa aagtattgat cgactgattg aagagcttcg
     5281 ttattattat ggtacgctgg tttttgttag tcatgatcgc tattttctag atgagctagc
     5341 atcgaaaatc tgggaagtaa aagacggaga aatccgagag ttttcgggga attatagtgc
     5401 ctatctcact caaaaggaat tggagaaaaa gactcagcta cgagaagcag agtcgatcat
     5461 gaaagagaaa aaacgattgg aaaaatcgat ccaagaaaag aaaaaacaag cggaaaagtt
     5521 ggaaaaagtg tccagtaaaa agaaaaagca acaaatcaga ctggatcggt tgtcttcctc
     5581 taaacaaaaa gacagtgtac aaaaagccat ccaaaagaat gcgaaaacat tagagagaag
     5641 actccaaaaa ataggagaaa caaccaagcc gcagcagatg aaacaaatcc gttttccagt
     5701 accaaaatct cttgaactcc acagccgcta tcccatcatg ggacaaaatg tccaattgga
     5761 aagaagtggg agaacattac tggtaaatgg tgattttcag ttttctttag gtaaaaaaat
     5821 cgcgattgtc ggtgaaaatg gttcaggtaa gacaacctta ttagaacata tccgcaaaca
     5881 aggagaagga atccttctct ctccgaaagt aagctttcaa gtatatcagc aaaagggtta
     5941 tcaaatgaca tctgaagaat ccatcattcg ttttgtcatg agacaaacag agttttcaga
     6001 atcgcttgtc cgtagtttgc tgaatcactt agggtttgct caggaaactt tgacgaaacc
     6061 gttatgtaca ttaagtgggg gagaagcgac ccgtctgacg attgctttgc tttttactaa
     6121 gccaagtaat gtgttgctgt tagatgaacc gactaatttt attgatatgg caacgatcga
     6181 agctttagag aagctaatgc aaatatatcc gggaacgatt ttgtttactt ctcatgattc
     6241 ttactttgtc gagcgtacgg ctgatgaagt ttatgaaata aaagggcaga aaataaaaaa
     6301 agtacttacg agaaattttt aaatgtgcta cgatgatttt cgtgacaaat ttaaaggagt
     6361 ggtacagatt gcctacaaat aaaaaagaag gaataatttt tacaacaatg atgtgttttt
     6421 tgatggtctt ggggatgagc gcgtataatt tatggctgca tcaagatctt catttatcaa
     6481 gtttgttgat tggtctagtg cctggattcg tggtggcttt cattttggat gtcttcatca
     6541 tcggtgtgct ggcgaaaaaa attgccttta aactaccagt aaatccagaa agcaaggtcc
     6601 aaatgatctt aactatttca tgcttgatgg ttttaggaat ggtgacatgt atgtctttat
     6661 tcggtgtgat catggaacaa ggaatacctg atcaattatt ctctgcttat atgaatgctt
     6721 ggaaaatgaa tgtgattgcc gctttaccgt tgcagttgat catcgttggc ccgctttcaa
     6781 gaagtgtgct aaaagcaatt caaagtaatg aatgatggaa cactgttatt tgaaaaaagg
     6841 ccttggcatg ctttttgtca tagtctcttg aatgcagttc tagaaaaata gtgatccgaa
     6901 cgataggttt tggcctgtcg ttcggatcgc tttttctttt ccaactgtat tgctctttta
     6961 ttgaagataa tttgctcttc aatagatatt tctagaaagg ttctgtttat cccttactca
     7021 aaaaactgct gcatttgttc tcttacttgc tgacaccatt ggagttcctg ctgacaaagc
     7081 ataattctgc gctggatcac aacataacgt ccaaattctt gtttcttgtc tagcgagagg
     7141 tcattcaatt ttttttgatt tttttgcatg attttgttca gctgttgttc tctagtgaat
     7201 agcagtccag tagctgttgt tttatcgagc gtcgaaaaag catagatttt tgctagaaat
     7261 tcatcttttt gcgtaggagc gggagtttcc tctttgatcc attcctctac aagagcacgt
     7321 ccttcatcag tcaagtggta gatctttttt tgagagtgct cgtctttgtc caaaacggtc
     7381 acataaccct gctggtctaa cttggcaagc gttgtataga tctgactgtg atgggcttcc
     7441 caaaaaattg tgatcagctg cttcaattca tacccagaac gtggtttgga taaaagcacg
     7501 cataataata tataggctaa agtattcata aaaatcctac ctctcgtctt attttcttta
     7561 ttatatcaca gtaaagtttc cacgttttac atgtgcatat tgacatgtaa aataaatcat
     7621 gtatgatgct aatagagaga aagggatgga aatattggag aatcaaatag atatacaaga
     7681 acaaacaaat gaagaaacaa aggggaaaag ttcagcgcta gttgtagcag ctacctgtat
     7741 gatcgctttt atgggagtgg gtctagtaga cccaatctta aaaacgattg ctgcacagtt
     7801 aaacgctacg ccagctgaaa cgaccctgtt atttactagt tacatgttag tcactggtgt
     7861 ggtgatgcta tttacaggtt tcttatctag tcggatcggt ttgaaaaaaa cattgatgac
     7921 aggattgttt atcatcgttg tattcgctgg tcttggcgga ttatctggga atatcggaat
     7981 gctgattggg ttacgtgccg gctggggtgt cgggaatgct ttatttgtct ctactgcttt
     8041 agcaacgatc gtcagtattt tagttggtaa cacagaaaaa gcaatcatga tgtatgaagc
     8101 agcacttggc tgcggaatgg ctgttggccc tctagtcggt ggggtcttgg gcagtggttc
     8161 atggcgttat ccattttttg gggtagcatt tttgatgttt tgcggtttta tagccctagc
     8221 ggtactttta cctgaaactg agaaaccaaa gagaaaagta ggcctttggg aaggtgtcaa
     8281 agcgttaagc aatcataaac ttcgttcagt tggactaatc gcattgttat ataatttcgg
     8341 attctttact ttacttgcgt atgcgccatt tttattgatt ggctattctg aattagaagt
     8401 cggttttgta ttttttggct ggggtgtgct tctggctatt gcttcgattt tcgttgcacc
     8461 ggctcttgag cggaagttta cgacttatcg gacaatttta gcagcatttg tcttattcat
     8521 tatttgtttg ctgttattag gagctggaac ttctatgcca gtccttgtac cgatcagcat
     8581 tgtgttagca ggatttttcc aaggaattat caatacactg ttgacgacga ttgctatgga
     8641 aataccaggt ctgcaacgaa acgttgcttc gtccagctat agttttgtcc gctttttcgg
     8701 tggagcttta gcaccattta ttgcaggtgt gattggtgaa catattggtg aaagctatgc
     8761 gttttatttt gcaggtgcca ttgttcttat cagtgtatgt tgtatcattt atcaccgtaa
     8821 ttattttgct acaaatgaag aagaataaga aatcagaggt tgtgaaaaag ctgtcctgca
     8881 tcaagtcata agaa
//
LOCUS       DARBFO010000265         5961 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.guided.128, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000265 DARBFO010000000
VERSION     DARBFO010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.guided.128"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1641
                     /locus_tag="SCK51_003121"
     CDS             <1..1641
                     /locus_tag="SCK51_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1253648.1"
                     /translation="IWNAKTVWIRKGTVINKMLDFLDLKYPSITSITEVPIEKAMTEY
                     RTYLTKRGVRITTTNYKITANQEKTPVKANSYYVTNLKQFMEFYENFYFDGEEWDKDV
                     WDRRNLPLPDDKVNPTQYEYTINFKGFRNTYFKQLVKRYCKLRLNMDSFSYVSDIAQR
                     LKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSELNMMGIKPSTITGRISILEGLFSTLL
                     RLEWDDVPSKILIYSEDYPKIPRAKPRFIDEFVLEQLNSHLDKLPEYIATMTMIVQEC
                     GMRISELCTLKKGCLLEDKDGDFFLKYYQWKMKKEHIVPISKEVALLIKVREDKVSEE
                     FPDSEYLFPRKDGSPLKQETFRGELNKLAYEQNIVDKSGEIYRFHAHAFRHTVGTRMI
                     NNGMPQHIVQKFLGHESPEMTSRYAHIFDETLKNEFTKFQEKLVTNNGDVLDLDEDNE
                     VDDVELQWFKKNINAQVLPNGYCRLPVVAGGCPHANACLDCTHFCTSKQFLPQHEEQL
                     ERTEELLAIAKDKQWQRQVETNSRVKERLEQIIGSLTG"
     gene            1648..2025
                     /locus_tag="SCK51_003122"
     CDS             1648..2025
                     /locus_tag="SCK51_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1253649.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            2179..2961
                     /locus_tag="SCK51_003123"
     CDS             2179..2961
                     /locus_tag="SCK51_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HEG1253650.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(3087..3818)
                     /gene="erm(A)"
                     /locus_tag="SCK51_003124"
     CDS             complement(3087..3818)
                     /gene="erm(A)"
                     /locus_tag="SCK51_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEG1253651.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(3876..3935)
                     /locus_tag="SCK51_003125"
     CDS             complement(3876..3935)
                     /locus_tag="SCK51_003125"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HEG1253652.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            4327..4989
                     /locus_tag="SCK51_003126"
     CDS             4327..4989
                     /locus_tag="SCK51_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1253653.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            5224..5367
                     /locus_tag="SCK51_003127"
                     /pseudo
     CDS             5224..5367
                     /locus_tag="SCK51_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            5517..5717
                     /locus_tag="SCK51_003128"
     CDS             5517..5717
                     /locus_tag="SCK51_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1253654.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2109 a          853 c         1138 g         1861 t
ORIGIN      
        1 atatggaatg caaaaactgt ctggattaga aaaggaacag taattaataa aatgttagat
       61 ttcttagatt taaagtatcc tagcattact tcaattactg aagttcctat tgaaaaagca
      121 atgacggagt atagaactta tttgacaaaa cggggtgtta gaattaccac tactaattat
      181 aagattactg ctaatcaaga aaaaacacct gtaaaagcta attcctacta tgttactaat
      241 ctaaaacaat ttatggagtt ttatgagaac ttttattttg atggagagga gtgggataaa
      301 gacgtttggg atagacgtaa cttacctttg ccagatgata aggttaaccc aacacaatat
      361 gaatatacaa ttaactttaa agggtttcgg aatacatatt ttaaacaact tgtaaaaaga
      421 tattgtaagt tgagattgaa catggatagc ttttcctatg taagtgatat tgcccaaaga
      481 cttaaagagt tctttaattt tctggacatg aaatttaaac aagttcagag agtacaccaa
      541 ttaacgagag tggaaattga agcatattta agtgaactaa acatgatggg aataaaacct
      601 agtacaataa ctgggaggat ctctatattg gaaggactat ttagtaccct tcttaggcta
      661 gaatgggatg atgttccttc caaaatatta atttattctg aggactatcc gaaaatacca
      721 agagcaaaac cacgctttat agatgaattc gtcctagagc aattgaacag tcatcttgat
      781 aaattacccg aatatatagc tacgatgact atgattgttc aagaatgtgg aatgaggata
      841 agtgaattgt gcaccttgaa aaaaggctgt ctattagagg acaaagatgg agatttcttt
      901 ttaaagtatt atcaatggaa aatgaaaaag gagcatatag ttccaatatc taaagaggta
      961 gctttactta ttaaagttcg ggaagataaa gtttcagagg aatttccaga tagtgaatac
     1021 ctctttccaa gaaaagatgg atcgccatta aaacaagaaa catttagagg tgagttaaat
     1081 aaattagctt atgagcaaaa tatagtggat aaatcaggtg agatttatag attccatgcc
     1141 catgcctttc gccatacagt aggaacaaga atgattaaca acgggatgcc ccagcatatt
     1201 gtgcagaaat ttttggggca tgaaagccca gaaatgacaa gcagatacgc tcatatcttt
     1261 gatgaaactc taaaaaatga atttactaaa tttcaggaaa aactggttac caataatgga
     1321 gatgtgcttg atctagatga agataatgaa gtcgatgatg tagagcttca atggttcaag
     1381 aaaaatataa atgcacaagt gcttccaaat ggttattgta gattgccagt agtagcaggt
     1441 ggttgtccac atgcgaatgc atgcttagat tgcactcact tctgtaccag taagcaattc
     1501 ttaccacagc acgaagaaca gttagagcgt acagaagagt tattagccat agctaaggat
     1561 aaacaatggc aaagacaagt agagactaat agccgtgtta aagagcgttt agaacaaatc
     1621 attggaagtt tgacggggta attatcaatg gataaacaag ttagaaatac aacagaaatt
     1681 gtacgtttgg cgaagcagaa atcaaaaaag acaagggaaa aagtagacaa agcgatttct
     1741 aaattttcga ttgaaggtaa agttattaat tttaattcaa tagcaaagga agctaatgtt
     1801 tctaaatcat ggctttataa ggaacacgat attaggcaaa gaatcgaatc ccttcgtgag
     1861 cgtcaaataa cagcaaatgt agtctcaaaa cccaagaaaa gttctcgttc ggaggaaatc
     1921 cttattaaaa ccttaaaaag aagagtaatg gaattagaaa aagaaaataa aaaattacag
     1981 aaccaaattc aaaaattata tggagatctg tataataaag aataattatt aatctgtaga
     2041 caaattgtga aaggatgtac ttaaacgcta acggtcagct ttattgaaca gtcatttagg
     2101 tatatgtcca atctagggta agtaaattga gtatcaatat aaactttata tgaacataat
     2161 cagttacgag gtgaaatcat gagcaatttg attaacggaa aaataccaaa tcaagcgatt
     2221 caaacattaa aaatcgtaaa agatttattt ggaagttcaa tagttggagt atatctattt
     2281 ggttcagcag taaatggtgg tttacgcatt aacagcgatg tagatgttct agtcgtcgtg
     2341 aatcatagtt tacctcaatt aactcgaaaa aaactaacag aaagactaat gactatatca
     2401 ggaaagattg gaaatacgga ttctgttaga ccacttgaag ttacggttat aaataggagt
     2461 gaagttgtcc cttggcaata tcctccaaaa agagaattta tatacggtga gtggctcagg
     2521 ggtgaatttg agaatggaca aattcaggaa ccaagctatg atcctgattt ggctattgtt
     2581 ttagcacaag caagaaagaa tagtatttct ctatttggtc ctgattcttc aagtatactt
     2641 gtctccgtac ctttgacaga tattcgaaga gcaattaagg attctttgcc agaactaatt
     2701 gaggggataa aaggtgatga gcgtaatgta attttaaccc tagctcgaat gtggcaaaca
     2761 gtgactactg gtgaaattac ctcgaaagat gtcgctgcag aatgggctat acctctttta
     2821 cctaaagagc atgtaacttt actggatata gctagaaaag gctatcgggg agagtgtgat
     2881 gataagtggg aaggactata ttcaaaggtg aaagcactcg ttaagtatat gaaaaattct
     2941 atagaaactt ctctcaatta ggctaatttt attgcaataa caggtgctta cttttaaaac
     3001 tactgattta ttgataaata ttgaacaatt tttgggaaga ataaagcgtc ctcttgtgaa
     3061 attagagaac gctttattac tttaatttag tgaaacaatt tgtaactatt gaaaatagaa
     3121 agaaattgtt ccttcgatag tttattaata ttagtgacat ttgcatgctt caaagcctgt
     3181 cggaattggt ttttagtgaa aagaacacga tattcacggt ttacccactt ataaacaaaa
     3241 gatcgatact ttttgtagtc cttctttgaa atcaatggtt gatgtcgttc aagaacaatc
     3301 aatacagagt ctacacttgg cttaggatga aaatatagtg gtggtacttt tttgagcatt
     3361 tttatatcca tctccaccat taatagtaaa cccaaagctc gttgcagatt ttgcaatctt
     3421 ttcgcaaatc ccttctcaac gataagatag ctatatttag cctgactttc aaaggtaatt
     3481 cttttgacaa tatccgtact gatgttataa ggaatattac catatatctt atagtttata
     3541 tgttttggga aggaaaattt tagaatatcc gtttgaatca cttttatatt ctcagagggg
     3601 tttaccgctt ctttagtcac ttgacataag cctccatcaa tttctatagc agtaactgat
     3661 cgactcattt tgactagctc tttggtaaaa tgtccttttc ctgatccgat ttctattacg
     3721 ttgtcttgtt tactgatatt cgtgtgattc aatatttctt ttacatgctt tttagaagta
     3781 ataaaatttt gcgtgtcttt agggtttttc tggttcatta taaccttctc cttactggtt
     3841 ataatgaact gacattaagc gttcattata accaattaat tttgatttgg ttgataacga
     3901 actttattaa taacaaatat agaaaaagta cccatacttt tatctccttt ccattttttt
     3961 cattatgaat gagataaagt aatttctact actgcgatac tggtgcacat aattgaaacc
     4021 tccttatgta actgaatatg attgtatcat cttttcattt tagaataaac ctttatatgt
     4081 aattttgcac ttgacacttc agatggacgt tttcttcaaa ataatatgtt ttgaaacttg
     4141 ataagagaag ccactgtctt tactaaaaag taaaggctgc gataaaggac agtgacttta
     4201 ataaagttgc actgttgtat tgtctttgga taaaattgtc taaagggaag ttttatcccc
     4261 aacgaaaaaa catctaaagt ggtaagtaat gaaagaatgt cgataaagta ggaatggggg
     4321 atctttttga atacaataga atcacagttt gataaggttg cagaagatta cgatttcgtg
     4381 aatgagcttt tgaatgatta ttcattcttt gtgtctaata tgtctccaaa gaaaggcaga
     4441 gcattagata tcggatgtgg ctcgggtttg ttagtggaga aattagcaag ttattatgat
     4501 gaagtggtag ggattgatat ttctaatcaa atgctcgatc ttgccaaatc taaacgtcaa
     4561 ctaacaaata cggtctatct gaatatgaat gcagaacaac ttaattttaa tgagaagttt
     4621 gattttattg taagccgaac aacctttcat catttggatg atatagccag cgtaatacag
     4681 caaatgaagg aactgttgaa tgaggaagga agaatagtta ttcttgataa tgtatctgaa
     4741 gtcgagacac cgcctaccta tgtttataaa ttaggggcaa tccaagagtt tttaccgcat
     4801 tgtttcaaat ttggaataaa aaatgcgatt agaatttata atcacaacac atctaaatca
     4861 tggcttgagc atttagcttc agataagtac ctatctgaac aaaattatta cgatttatat
     4921 gaaaagttgt tgcctggatg ccaattccat aaaatgggtt gggcaatggg ggttgtctgg
     4981 acaaaataac ttaaagctgt ggctttgaaa agttgaattt gtgattgttt tcagagctat
     5041 cgggtgcttt agtataaata acaatgaata aaaaatcagc ctgtatctta tgttactatt
     5101 aagtaattta agatacaggt tttactattt ttgaaataag taccttccac ttcaaataca
     5161 tgtattcaaa gtgaaagtat tgatttaaat gattcttttg taatttactc ttgataatat
     5221 gaaattccat cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg
     5281 atggggatca ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga
     5341 gaagaaggaa tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt
     5401 acaattatat tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg
     5461 tacacgagca atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg
     5521 aaaacggaac agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag
     5581 atggaagcga cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag
     5641 aagaaggtca agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa
     5701 acgttgtaaa agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg
     5761 agagaacaaa gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt
     5821 atttttttgt atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca
     5881 aggaataaga acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca
     5941 ggacaaggtg ctgatttcat a
//
LOCUS       DARBFO010000266         5613 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.guided.132, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000266 DARBFO010000000
VERSION     DARBFO010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.guided.132"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(40..1026)
                     /locus_tag="SCK51_003129"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="SCK51_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="SCK51_003130"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="SCK51_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEG1253655.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="SCK51_003131"
     CDS             1851..3005
                     /locus_tag="SCK51_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEG1253656.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="SCK51_003132"
     CDS             3220..4188
                     /locus_tag="SCK51_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEG1253657.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="SCK51_003133"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="SCK51_003133"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEG1253658.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5583
                     /locus_tag="SCK51_003134"
                     /pseudo
     CDS             5218..5583
                     /locus_tag="SCK51_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
BASE COUNT         1697 a         1091 c         1262 g         1563 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     5461 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     5521 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcggttct
     5581 gttgcaaagt tttaaataaa gaataaaatc cct
//
LOCUS       DARBFO010000267         1916 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.guided.194, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000267 DARBFO010000000
VERSION     DARBFO010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.guided.194"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..859)
                     /locus_tag="SCK51_003135"
     CDS             complement(<1..859)
                     /locus_tag="SCK51_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253659.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQN"
     gene            complement(1573..1863)
                     /locus_tag="SCK51_003136"
     CDS             complement(1573..1863)
                     /locus_tag="SCK51_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1253660.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          653 a          354 c          259 g          650 t
ORIGIN      
        1 agttttgaca tttcctatat gttagaagga aaattaagaa aaatagcaat aaaaataaga
       61 tattcatgtc taaaaaaaac ggctttccaa atgatgaaat acctaaatct aacttattaa
      121 gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca gcacgtaatg
      181 cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt gaaaatactg
      241 ataacttata attatatcgt ccattgacaa tcaaatataa actaactata cttgttagaa
      301 caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa ggaatactta
      361 cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac ggataaaaag
      421 ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc caaccggttt
      481 cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat aaatttttaa
      541 catttatttt ctcaaaaaca tacttgcaaa gtaatactac aattcctaac ataatcgtag
      601 aatttaataa tttccatagc ataaagtgtt tagaaaagaa cattaagaat agttctataa
      661 taattcttga actccatgtc ttataatgtt ctatcgaagc acccaaatag ccaagttcat
      721 ctaatctact tgcataaact acgtcatccg ctctcatagt cggcatatac atattaaaga
      781 gtaaaaagat accccaaatc cctattagta acaagatcag ttctttgtta ttcgttattt
      841 ttctattatg tttgttcatt tttatactcc ttcttatatt tcctaataga gtataacata
      901 gctttaagca ttacttaaag ataacttaaa agttatcttt taaatactta attgttaaat
      961 accttatttg atgcaaaaga aatctacgat agtcaacaaa aaaattttca ttatatccag
     1021 aaaaattttt cttagttaag gacctatgga ttgcagtaat gcagaattcg tcgaataaac
     1081 tgccaaagac atacaatcat attgctttta aaattgacga acaagatata gattcgtttg
     1141 tctcaaaaat ccaaatgtta ggtttaactg tcgaaccagg acgttctcgt gtgaaaggcg
     1201 aggcttcttc aatttacttt tacgactatg ataatcacct cttcgagtta cataccggta
     1261 ctctccagga acgtttaaaa tcctacaatt ccactacctg agactagtaa tgactagtct
     1321 ctttttatgg cctctgatcc ccccattgag cgaatactaa tagggcttcg aattgatctg
     1381 ttcaatcttt aagcaatatc cctcaaatga gtttggaaag tttagtgatt catcacaaat
     1441 acttaaatac atgtagttat atatagattg aggttcttgt gagcgtatgc tcgtgtgggt
     1501 tcaaatccca tcgtctgcat ccatctttcc aaaacaagcg gctaggacat ttttgtctca
     1561 gccgcttgtt ttctagttta ttctttcatc ttgtcggacc ttttttcttg ggtatagact
     1621 tttctcatat tctaccacaa ttttgggttt ccctaacaca tgataggatc tcacaacaaa
     1681 ttgctttcta ttattataat aacctgtaac gactaacttc atcccttcgt ctacttcagc
     1741 taggaagttc aaactatggg aagcaatcaa acaattgacc ccatttaaac tgaaacggat
     1801 caacggtctg gcactaactt gcaaagtacg gatcttatct actgttccat attttttctc
     1861 cataaccttc acctcaatac cattatacga acatacattc cctttgtaaa gaagaa
//
LOCUS       DARBFO010000268         1504 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.guided.208, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000268 DARBFO010000000
VERSION     DARBFO010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.guided.208"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            262..345
                     /locus_tag="SCK51_003137"
                     /pseudo
     CDS             262..345
                     /locus_tag="SCK51_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="SCK51_003138"
     CDS             394..477
                     /locus_tag="SCK51_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HEG1253661.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="SCK51_003139"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="SCK51_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEG1253662.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DARBFO010000269         1421 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.guided.212, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000269 DARBFO010000000
VERSION     DARBFO010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.guided.212"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1350
                     /locus_tag="SCK51_003140"
     CDS             <1..1350
                     /locus_tag="SCK51_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HEG1253663.1"
                     /translation="RDFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQ
                     IYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANA
                     VILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMK
                     YLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLN
                     TNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFL
                     RQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVF
                     EGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFG
                     EDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          597 a          114 c          239 g          471 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg aatcattaaa aaaacattat
       61 acagagcctt gggaagatga agtttttaga gtaattattg aatataacaa tgttcctatt
      121 ggatatggac aaatatataa aatgtatgat gagttatata ctgattatca ttatccaaaa
      181 actgatgaga tagtctatgg tatggatcaa tttataggag agccaaatta ttggagtaaa
      241 ggaattggta caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct
      301 aatgcagtta ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa
      361 tctggtttta gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa
      421 gattgttatt taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa
      481 tatttaattg agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt
      541 ggttatgata gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt
      601 actaataaga aaaaaggtta tgcaaaagaa aaagcaatat ataatttttt aaatacaaat
      661 ttagaaacta atgtaaaaat tcctaatatt gaatattcgt atattagtga tgaattatct
      721 atactaggtt ataaagaaat taaaggaact tttttaacac cagaaattta ttctactatg
      781 tcagaagaag aacaaaattt gttaaaacga gatattgcca gttttttaag acaaatgcac
      841 ggtttagatt atacagatat tagtgaatgt actattgata ataaacaaaa tgtattagaa
      901 gagtatatat tgttgcgtga aactatttat aatgatttaa ctgatataga aaaagattat
      961 atagaaagtt ttatggaaag actaaatgca acaacagttt ttgagggtaa aaagtgttta
     1021 tgccataatg attttagttg taatcatcta ttgttagatg gcaataatag attaactgga
     1081 ataattgatt ttggagattc tggaattata gatgaatatt gtgattttat atacttactt
     1141 gaagatagtg aagaagaaat aggaacaaat tttggagaag atatattaag aatgtatgga
     1201 aatatagata ttgagaaagc aaaagaatat caagatatag ttgaagaata ttatcctatt
     1261 gaaactattg tttatggaat taaaaatatt aaacaggaat ttatcgaaaa tggtagaaaa
     1321 gaaatttata aaaggactta taaagattga ttatataata tatgaaaagc tattataaaa
     1381 gacggttctg ttgcaaagtt ttaaatctac tatcaaataa g
//
LOCUS       DARBFO010000270         1350 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.guided.217, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000270 DARBFO010000000
VERSION     DARBFO010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.guided.217"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            47..298
                     /locus_tag="SCK51_003141"
                     /pseudo
     CDS             47..298
                     /locus_tag="SCK51_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="SCK51_003142"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="SCK51_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEG1253664.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DARBFO010000271         1225 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.guided.223, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000271 DARBFO010000000
VERSION     DARBFO010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.guided.223"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            510..1058
                     /gene="aac(6')"
                     /locus_tag="SCK51_003143"
     CDS             510..1058
                     /gene="aac(6')"
                     /locus_tag="SCK51_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEG1253665.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          435 a          179 c          249 g          362 t
ORIGIN      
        1 agagtgatcg atctgactgc ggctgtagat ggagtagagg tgaacctaag acaggataag
       61 tgatgaataa ggtggcatga acaccttgaa atcatcaata agtatcctaa aaacgaacaa
      121 gtacaaaaac attgaaaacg acaataatga atttttgttc ctcttagtat ttctaccgat
      181 tccctttgct tttttgtaat tatctaaaaa ttctaaaaat taagattatt atatgaaaac
      241 aggttttatt tgtcaattca taattggttt tttttaaagc aaaagataat ctatatcatg
      301 atgaacaaac agagtttttt cctccaattg atggctttcc tgatctttcc tattttgaat
      361 aaagtgtttc tgtataattt ttttatttta taagtaagat aaataatgaa agaatagaga
      421 tactgattgg tagctaaaga tttttgagcg agaacatttc gttttttcaa aaggactgtt
      481 agaataagca agaacaaggg ggaagacgta tgataatcag tgaatttgac cgtaataatc
      541 cagtattgaa agatcagctt tctgatttac tgagactgac ttggccggaa gaatatggag
      601 acagctcggc agaagaagta gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg
      661 tagaccaaga tgagttagta ggatttattg gtgcaatccc tcaatacggt atcacaggtt
      721 gggaattgca tccattagtt gtagaaagct cccgacgaaa gaaccaaata ggtactcgat
      781 tagtcaatta cttagaaaaa gaagtagctt ccagaggagg aatcacgatt tatttaggta
      841 cggatgattt agaccatgga acaacgttaa gtcaaacgga cctgtatgag catacatttg
      901 ataaagtggc ttccatccag aaccttcgtg aacatccgta tgaattctat gaaaaattag
      961 gttataaaat cgtaggtgtc ttaccaaatg caaatggctg ggacaaaccg gatatttgga
     1021 tggcaaaaac gattattcct cgaccaaatt ctcaataata aaaagaggtt gtgacagaag
     1081 taatcagctt caagaaataa gaaggaattt acgaaaatta cttcttgaat ttttgtgaaa
     1141 ttccaactta tttccgaaga agctactttt ggaacaccgt gtaccagagg ttgtgaaaaa
     1201 gtcgcttagt ttcgagtagt aagaa
//
LOCUS       DARBFO010000272         1194 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3743
            SAMEA114569755-rid20111973.guided.226, whole genome shotgun
            sequence.
ACCESSION   DARBFO010000272 DARBFO010000000
VERSION     DARBFO010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569755
            Sequence Read Archive: ERR12203579
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972594.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972594.1
            Genome Coverage       :: 119x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:24:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,144
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,005
            CDSs (with protein)               :: 3,005
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 25 of 75
            Pseudo Genes (incomplete)         :: 37 of 75
            Pseudo Genes (internal stop)      :: 20 of 75
            Pseudo Genes (multiple problems)  :: 6 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569755-rid20111973.guided.226"
                     /isolate="VRE3743"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            259..>1194
                     /gene="vanY"
                     /locus_tag="SCK51_003144"
     CDS             259..>1194
                     /gene="vanY"
                     /locus_tag="SCK51_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HEG1253666.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          426 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaat
//
